Collection of peripheral blood progenitor cells with an automated leukapheresis system.
BACKGROUND
Apheresis devices designed for the collection of mature blood elements are being used for the collection of peripheral blood progenitor cells (PBPCs). The collection of PBPCs differs from that of other cells in the rarity of the target cell and in the fact that donors may undergo several days of collection. A consequence of this process may be a depletion of blood cells such as platelets from the blood. The disposable set and operating software for an apheresis device (Spectra, COBE BCT) was modified by the manufacturer to automate the collection of PBPCs and reduce the collection of unwanted blood cells.


METHODS
A study was initiated to compare the collection of PBPCs with the new device, the AutoPBSC (version [V]6.0 with AutoPBSC tubing set), and that with the MNC (mononuclear cell) procedure (V4.7 with white cell tubing set), for patients and healthy donors.


RESULTS
Patients whose blood was processed by either theV6.0 orV4.7 procedure achieved the target dose of 5 x 10(6) CD34+ cells per kg of patient weight in similar numbers of procedures, even though the calculated collection efficiency for CD34+ cells using the automated V6.0 procedure was significantly less than that with the V4.7 procedure for both allogeneic donors and patients donating PBPCs. The collection efficiency for platelets was lower with the V6.0 procedure, and components collected in this manner contained fewer platelets. Apheresis by the V6.0 procedure required 30 to 60 more minutes per procedure than apheresis by the V4.7 procedure. Review of engraftment kinetics after transplantation did not reveal any effect of the collection procedure on recipients of either allogeneic or autologous transplants.


CONCLUSIONS
The collection efficiencies of the V6.0 procedure for both CD34+ cells and mature blood cells are lower than those of the V4.7 procedure. The lower collection efficiency for platelets results in a smaller drop in peripheral blood platelet count after the procedure. The automated features of the V6.0 procedure may simplify PBPC collection, but this procedure requires a longer apheresis.
Rowley
S D
SD
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA. srowley@fhcrc.org
Prather
K
K
Bui
K T
KT
Appel
M
M
Felt
T
T
Bensinger
W I
WI
D000328:Adult; D018952:Antigens, CD34 / Q000097:blood; D001792:Blood Platelets / Q000166:cytology; D001804:Blood Transfusion, Autologous; D005260:Female; D006801:Humans; D007937:Leukapheresis / Q000295:instrumentation*; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000166:cytology*; D013997:Time Factors; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous
D018952:Antigens, CD34