[Use of high-density filters ("DNA arrays") for gene expression analysis in human tissues].
Most technical strategies for the analysis of gene expression in tissues are able to study only one protein or RNA product at the same time. A new recent method referred to as <<DNA array>> or <<high density filter>> is able to analyze simultaneously several hundreds of different genes. The DNA array is a nylon membrane on which are spotted equal amounts of cDNAs corresponding to different genes. This filter is hybridized with a <<complex>> probe synthesized with mRNA derived from the tissue analyzed. The result gives a global profile of gene expression within the tissue and allows quantitative and comparative analysis between different tissues or cell types.
Oddou
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S
U119 de l'INSERM, 27 Bd Lei Roure, 13009 Marseille.
Devilard
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E
Dales
J P
JP
Hassoun
J
J
Birg
F
F
Xerri
L
L
D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D010336:Pathology / Q000295:instrumentation / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*
D018076:DNA, Complementary; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D012333:RNA, Messenger