Complete amino acid sequence determination of the major allergen of peach (Prunus persica) Pru p 1.
The major protein allergen of peach (Prunus persica), Pru p 1, has recently been identified as a lipid transfer protein (LTP). The complete primary structure of Pru p 1, obtained by direct amino acid sequence and liquid chromatography-mass spectrometry (LC-MS) analyses with the purified protein, is described here. The protein consists of 91 amino acids with a calculated molecular mass of 9178 Da. The amino acid sequence contains eight strictly conserved cysteines, as do all known LTPs, but secondary structure predictions failed to classify the peach 9 kDa protein as an 'all-alpha type', due to the high frequency of amino acids (nine prolines) disrupting alpha helices. Although the sequence similarity with maize LTP is only 63%, out of the 25 amino acids forming the inner surface of the tunnel-like hydrophobic cavity in maize ns-LTP 16 are identical and 7 similar in the peach homolog, supporting the hypothesis of a similar function.
Pastorello
E A
EA
Allergy Center, Department of Internal Medicine, Ospedale Maggiore IRCCS, Milan, Italy.
Ortolani
C
C
Baroglio
C
C
Pravettoni
V
V
Ispano
M
M
Giuffrida
M G
MG
Fortunato
D
D
Farioli
L
L
Monza
M
M
Napolitano
L
L
Sacco
M
M
Scibola
E
E
Conti
A
A
D000485:Allergens / Q000032:analysis / Q000737:chemistry*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D052179:Antigens, Plant; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry*; D019685:Rosales / Q000737:chemistry*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D000596:Amino Acids; D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; C403252:Pru p 1 allergen; C511920:lipid transfer proteins, plant; D010450:Endopeptidases; D008666:Metalloendopeptidases; C058921:endoproteinase Asp-N