[The effect of serum starvation on the efficacy of the nuclear reprogramming of rabbit embryonic fibroblasts].
Successful transplantation of mammalian nuclei from differentiated cells has become possible after the application of original methods directed at the synchronization of cell cycles of the donor cell and recipient cytoplasm. We obtained a line of rabbit fetal fibroblasts which was used to study factors affecting the success of reprogramming. The nuclei of fetal fibroblasts (up to the 10th passage inclusive) proved to be capable of reprogramming and ensuring development of the cloned embryos until the preimplantation stages. The influence of synchronization of the cell cycles of the nucleus donor and recipient on the efficiency of reprogramming was studied. The rate of development of the cloned rabbit embryos to the morula-blastocyst stage reached 67% when the nuclei used were from stationary culture cells (G0-phase).
Galat
V V
VV
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Oblast, Russia.
Lagutina
I S
IS
Mezina
M N
MN
Prokof'ev
M I
MI
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002453:Cell Cycle; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D011817:Rabbits
D016895:Culture Media, Serum-Free