Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins.
We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.
Lyman
S K
SK
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
Gerace
L
L
Baserga
S J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D012261:Ribonucleoproteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D020718:Ribonucleoproteins, Small Nucleolar / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C438199:NOP58 protein, S cerevisiae; C499062:NOP58 protein, human; C499060:Nop58 protein, rat; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D020718:Ribonucleoproteins, Small Nucleolar; D029701:Saccharomyces cerevisiae Proteins