Phenotyping of porcine apolipoprotein E using isoelectric focusing and localization of the APOE gene within the halothane-susceptibility linkage group.
A method has been developed for the phenotyping of pig apolipoprotein E. It was based on isoelectric focusing of sialidase-treated and delipidated blood plasma followed by immunoblotting. As the first antibody the anti-human apolipoprotein E antibody was used. The genetic polymorphism of pig apolipoprotein E appeared to be controlled by three alleles at one locus. A two-point linkage analysis was performed between certain loci belonging to the halothane linkage group and the APOE locus on material comprising 9 Zlotnicka Spotted boars, 30 Polish Large White sows, and their 160 offspring. A tight linkage between the A1BG and the APOE loci was documented (theta = 0.038).
Kurył
J
J
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Mroków, Poland.
D000818:Animals; D001057:Apolipoproteins E / Q000097:blood / Q000187:drug effects / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage / Q000187:drug effects; D014644:Genetic Variation; D006221:Halothane / Q000494:pharmacology; D007525:Isoelectric Focusing; D008297:Male; D010375:Pedigree; D010597:Pharmacogenetics; D010641:Phenotype; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics*
D001057:Apolipoproteins E; D006221:Halothane