title: Up- and down-regulation of BDNF mRNA in distinct subgroups of rat sensory neurons after axotomy.
abstract: Anterograde transport of BDNF is enhanced by axotomy in the rat sciatic nerve. However, the changes in BDNF gene expression in dorsal root ganglion (DRG) neurons after axotomy are not known. We examined this issue using in situ hybridization histochemistry. BDNF mRNA was detected in 35-40% of DRG neurons (L5) of control rats. Most of these neurons are small. BDNF gene expression in these neurons was down-regulated after application of capsaicin to the sciatic nerve. Transection of the sciatic nerve induced the up-regulation of BDNF mRNA. The intensely labeled neurons were mainly large and immunoreactive for neuropeptide Y. These results suggest that up- and down-regulation of BDNF gene expression in distinct subgroups of rat DRG neurons are caused by damage to the peripheral nerve.
authors.affiliation: Department of Physiology, Kansai College of Oriental Medicine, Sennan, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kashiba
authors.forename: E
authors.initials: E
authors.lastname: Senba
mesh_terms: D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000818:Animals; D019771:Axotomy; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D002211:Capsaicin / Q000494:pharmacology; D015536:Down-Regulation / Q000235:genetics; D005727:Ganglia, Spinal / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009475:Neurons, Afferent / Q000737:chemistry / Q000378:metabolism*; D009478:Neuropeptide Y / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology* / Q000601:surgery; D015854:Up-Regulation / Q000235:genetics
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009478:Neuropeptide Y; D012333:RNA, Messenger; D002211:Capsaicin