Identification of the mitochondrial carnitine carrier in Saccharomyces cerevisiae.
The mitochondrial carrier protein for carnitine has been identified in Saccharomyces cerevisiae. It is encoded by the gene CRC1 and is a member of the family of mitochondrial transport proteins. The protein has been over-expressed with a C-terminal His-tag in S. cerevisiae and isolated from mitochondria by nickel affinity chromatography. The purified protein has been reconstituted into proteoliposomes and its transport characteristics established. It transports carnitine, acetylcarnitine, propionylcarnitine and to a much lower extent medium- and long-chain acylcarnitines.
Palmieri
L
L
Department of Pharmaco-Biology, University of Bari, Italy.
Lasorsa
F M
FM
Iacobazzi
V
V
Runswick
M J
MJ
Palmieri
F
F
Walker
J E
JE
D000108:Acetylcarnitine / Q000378:metabolism; D026905:Amino Acid Transport Systems; D002331:Carnitine / Q000031:analogs & derivatives / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity; D013997:Time Factors
D026905:Amino Acid Transport Systems; C403382:CRC1 protein, S cerevisiae; D002352:Carrier Proteins; D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C116917:acylcarnitine; C003223:propionylcarnitine; D000108:Acetylcarnitine; D002331:Carnitine