Characterization of a novel porcine endothelin(B) receptor splice variant.
Screening of porcine cerebellum cDNA library with porcine endothelin(B) (ET(B)) receptor cDNA revealed a novel ET(B) receptor cDNA that is distinctly different from the wild-type ET(B) receptor in length and the amino acid sequence at the C-terminal end. This sequence appears to represent alternate splicing of the carboxy terminal end of ET(B) receptor, resulting in a polypeptide of 429 amino acids in length, which is 14 amino acids shorter than the wild-type porcine ET(B) receptor. Characterization of the wild-type and alternately spliced ET(B) receptors expressed in COS cells revealed that both receptors displayed very similar binding [apparent dissociation constant (K(d)) and maximum binding (B(max)) for (125)I-ET-1 were 71 pM and 1.6 pmol/mg protein for wild-type and 81 pM and 1.2 pmol/mg protein for splice variant ET(B) receptors] as well as functional properties. These data suggest that the differences in the amino acids at the C-terminal end had no effect on binding or functional coupling of these alternately spliced ET(B) receptors.
Nambi
P
P
Department of Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA, USA.
Wu
H L
HL
Ye
D
D
Gagnon
A
A
Elshourbagy
N
N
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D019556:COS Cells / Q000378:metabolism*; D002531:Cerebellum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D015723:Gene Library; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D011869:Radioligand Assay; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000737:chemistry* / Q000145:classification; D013552:Swine
D010716:Phosphatidylinositols; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; D015240:Phorbol 12,13-Dibutyrate