title: MHC-restricted protection of cats against FIV infection by adoptive transfer of immune cells from FIV-vaccinated donors.
abstract: The role of cellular immunity in vaccine protection against FIV infection was evaluated using adoptive cell transfer studies. Specific-pathogen-free cats received two adoptive transfers of washed blood cells from either vaccinated or unvaccinated donors with varying MHC compatibility at 1-week intervals, and a homologous FIV(Pet) challenge 1 day after the first adoptive transfer. FIV-specific CTL, IFN-gamma production, and proliferation responses were detected in the PBMC from the vaccinated donors. Seven of eleven (64%) recipients of cells from half-matched/vaccinated donors remained negative for FIV-antibodies after FIV challenge and four of those were completely protected. Two of two recipients of cells from MHC-identical/vaccinated donors were completely protected. All recipients of cells from unrelated/vaccinated, half-matched/unvaccinated, or unrelated/unvaccinated donors were unprotected. Thus, protection mediated by adoptive transfer of immunocytes from vaccinated cats was MHC-restricted, occurred in the absence of antiviral humoral immunity, and correlated with the transfer of cells with FIV-specific CTL and T-helper activities.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32611, USA.
authors.forename: R
authors.initials: R
authors.lastname: Pu
authors.forename: M
authors.initials: M
authors.lastname: Omori
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.forename: S L
authors.initials: SL
authors.lastname: Rine
authors.forename: B A
authors.initials: BA
authors.lastname: Lewis
authors.forename: E
authors.initials: E
authors.lastname: Lipton
authors.forename: J K
authors.initials: JK
authors.lastname: Yamamoto
mesh_terms: D019264:Adoptive Transfer / Q000379:methods* / Q000706:statistics & numerical data; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000379:methods; D002415:Cats; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D007111:Immunity, Cellular / Q000276:immunology; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000637:transplantation*; D008213:Lymphocyte Activation / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014019:Tissue Donors; D019562:Viral Load; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines; D007371:Interferon-gamma