Changes in the free amino acid composition with maturity of the noble cultivar of Vitis rotundifolia Michx. grape.
The changes in amino acid composition that occur with maturity of the Noble cultivar of the Vitis rotundifolia Michx. (muscadine) grape were determined by HPLC. Eighteen amino acids were identified. Histidine was the most prominent amino acid followed by alanine. The concentrations of most of the major amino acids (alanine, glycine, histidine, valine, isoleucine, aspartic acid, and serine) were highest at verasion. Glutamine and threonine contents dropped sharply after fruit set, while those of arginine and proline increased gradually with maturity and ripening. Tyrosine content increased gradually with maturity and ripening following a slight drop after fruit set. In ripe grapes, seeds contained most of the amino acids in mature grapes (50%) followed by the pulp (23%), the juice (15%), and the skin (11%). Alanine, histidine, and arginine were the principal amino acids identified in the juice. Alanine, histidine, arginine, valine, glutamine, aspartic acid, proline, serine, and threonine accounted for about 90% of the amino acids in the pulp. In seeds, alanine, proline, asparagine, and histidine accounted for over 55% of the amino acids, while alanine and histidine were found to be the predominant free amino acids in the skin. The profile indicates some differences in the changes in amino acid composition with berry maturity and relative amounts of amino acids present in muscadine compared to those in nonmuscadine grape species.
Lamikanra
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Center for Viticultural Science and Small Farm Development, Florida A&M University, Tallahassee, Florida 32307, USA. sola@nola.srrc.usda.gov
Kassa
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D000596:Amino Acids / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D019685:Rosales / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D000596:Amino Acids