Differential expression of novel abundant and highly regionalized mRNAs of the canine epididymis.
Three novel gene products have been cloned by differential screening of a dog epididymis cDNA library as part of a global appraisal of specific gene expression in the epididymis. The predicted proteins were provisionally named CE8-CE10 (for canine epididymal gene products 8-10). Northern blot analyses and in situ transcript hybridization confirmed that the cDNAs were all derived from tissue-specific, moderately to highly abundant mRNAs of the epididymal epithelium, showing a distinct regionalized expression pattern within the epididymal duct. Their sequences predict (i) a novel 19 kDa member of the Ly-6-domain protein superfamily (CE8), (ii) an approximately 30 kDa protein with multiple membrane-spanning regions (CE9), and (iii) a novel approximately 13 kDa single whey acidic protein domain protein (CE10). Closely related, cross-hybridizing gene products were abundant in the epididymis of stallions and bulls, but not in rodents or men. Changes in mRNA frequency were observed that specifically correlated with a cryptorchid situation and with the age of the dogs. Gene products restricted to the caput epididymidis were affected by both conditions, while those with a wider regional distribution were not.
Gebhardt
K
K
IHF Institute for Hormone and Fertility Research, University of Hamburg, FRG.
Ellerbrock
K
K
Pera
I
I
Ivell
R
R
Kirchhoff
C
C
D000375:Aging / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface; D001483:Base Sequence; D051926:Basigin; D001798:Blood Proteins / Q000235:genetics; D015152:Blotting, Northern; D003456:Cryptorchidism / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004285:Dogs / Q000378:metabolism*; D004822:Epididymis / Q000378:metabolism*; D015723:Gene Library; D017403:In Situ Hybridization; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D008894:Milk Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D017422:Sequence Analysis, DNA
D000954:Antigens, Surface; D001798:Blood Proteins; C079432:Bsg protein, rat; C402684:CE10 protein, Canis familiaris; C402683:CE8 protein, Canis familiaris; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008894:Milk Proteins; D012333:RNA, Messenger; D051926:Basigin; D004247:DNA