Effect of bone morphogenetic protein-12 gene transfer on mesenchymal progenitor cells.
Recombinant adenovirus mediated human bone morphogenetic protein-12 gene transfer induced tendon and cartilage-like tissue formation in vivo. The recombinant adenovirus with the human bone morphogenetic protein-12 gene was constructed, and mature human bone morphogenetic protein-12 expression mediated by adenovirus gene transfer was detected by specific antibody. Unlike bone morphogenetic protein-2 gene transfer, bone morphogenetic protein-12 gene transferred mesenchymal progenitor cell line C3H 10T1/2 showed no change of alkaline phosphatase activity, which is the mark of cell differentiation into osteoblastic phenotype. Injection of bone morphogenetic protein-12 gene transferred C3H 10T1/2 cells into nude mice thigh muscles induced tendon and cartilage-like tissue formation. The results indicate bone morphogenetic protein-12 has different effects on mesenchymal progenitor cell differentiation, and it may influence the cell differentiation into a nonosteoblast lineage.
Lou
J
J
Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, MO 63110, USA.
Tu
Y
Y
Ludwig
F J
FJ
Zhang
J
J
Manske
P R
PR
D000256:Adenoviridae / Q000235:genetics; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000494:pharmacology; D002356:Cartilage / Q000187:drug effects; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000235:genetics; D018014:Gene Transfer Techniques; D006614:Hindlimb; D006801:Humans; D008648:Mesoderm / Q000166:cytology* / Q000201:enzymology; D051379:Mice; D008809:Mice, Inbred C3H; D011995:Recombination, Genetic; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology; D013710:Tendons / Q000187:drug effects
D019485:Bone Morphogenetic Proteins; D000469:Alkaline Phosphatase