Primary structure of guinea pig plasma prekallikrein.
A full length guinea pig plasma prekallikrein (PK) cDNA was cloned from a liver cDNA library. The nucleotide sequence with 2242 bp was analyzed and the amino acid sequence with 618 residues was deduced. Kallikrein was purified from guinea pig plasma and cleavage site in the activation was determined. The amino acid sequence around the cleavage site -368Ile-Asp-Ala-Arg-Ile-Val-Gly-375Gly- differed from that of the human PK -368Thr-Ser-Thr-Arg-Ile-Val-Gly-375Gly-. Protease substrates containing penta-peptides which mimicked the sequence of the cleavage sites from P3 to P2' of guinea pig Hageman factor (HF) and PK were synthesized, and kinetic analyses of the hydrolysis by guinea pig activated HF (HFa) and kallikrein were carried out. The combination between HFa and the PK mimicking peptide provided the best kinetics. These results in part explain why the cascade activation of PK by HFa is predominant in the guinea pig system.
Shibuya
Y
Y
Department of Laboratory Medicine, School of Medicine, Kumamoto University, Japan.
Semba
U
U
Nishino
N
N
Khan
M M
MM
Tanase
S
S
Okabe
H
H
Yamamoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D006168:Guinea Pigs; D006801:Humans; D006868:Hydrolysis; D007610:Kallikreins / Q000097:blood / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis; D011288:Prekallikrein / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D018076:DNA, Complementary; D009842:Oligopeptides; D011288:Prekallikrein; D007610:Kallikreins