[Properties of stored platelet concentrates: effect of the suspension media and type of pouch used].
OBJECTIVE
We have evaluated in vitro the effects of using plasma (group A) or PAS-2 (group B) in storing platelets, regarding as well the type of container (conventional polyolefin--PL732--or new oxygen permeable platelet containers--PL2410, Compoflex--), on the metabolism of platelet concentrates--PC--from pools of five buffy coats.


METHODS
87, A and B pools of PCs collected in two types of bags were studied. The samples were taken on days 2, 5 and 7, and cell counts, pH, glucose, platelet activation (% CD62) and aggregation response were measured.


RESULTS
In group A, when we compared the two types of bags, we observed a difference (p < 0.01) in values of pH and glucose on day 5. These values were more advantageous in new oxygen permeable platelet containers. The rest of the parameters analysed didn't show significant differences. When we compared the PL-2410 containers from groups A and B, we found a lower level of glucose (p < 0.01) in group B, although the levels of glucose in this group on days 5 and 7 of storage were sufficient to support the platelet metabolism.


CONCLUSIONS
The use of new oxygen permeable polyolefin containers and additive solutions, PAS-2, allows us to obtain pools of PC with suitable metabolic parameters during storage.
Castrillo
A
A
Centro de Transfusión de Galicia, Santiago de Compostela, A Coruña.
Castro
A
A
Cid
J
J
Adelantado
M
M
Eiras
A
A
Flores
J
J
Solla
E
E
Varela
J
J
García-Villaescusa
R
R
D000328:Adult; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D001793:Blood Preservation / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D010100:Oxygen; D010949:Plasma; D010969:Plastics; D011090:Polyenes; D012996:Solutions / Q000494:pharmacology; D013696:Temperature
D010969:Plastics; D011090:Polyenes; D012996:Solutions; C035051:PL 732; D010100:Oxygen