Ribose 1,5-bisphosphate regulates rat kidney cortex phosphofructokinase.
Phosphofructokinase (EC 2.7.1.11) is a major enzyme of the glycolytic pathway, catalyzing the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate. In this study, we demonstrated the effect of ribose 1,5-bisphosphate on phosphofructokinase purified from rat kidney cortex. Ribose 1,5-bisphosphate relieved the phosphofructokinase from ATP inhibition and increased the affinity for fructose 6-phosphate at nanomolar concentrations. These activating effects of ribose 1,5-bisphosphate were enhanced in the presence of AMP. Ribose 1,5-bisphosphate reduced the inhibition of the phosphofructokinase induced by citrate. These results suggest that ribose 1,5-bisphosphate is an activator of rat kidney cortex phosphofructokinase and synergistically regulates the enzyme activity with AMP.
Ozeki
T
T
Department of Physiology, Nihon University School of Dentistry, Chiba, Japan.
Mitsui
Y
Y
Sugiya
H
H
Furuyama
S
S
D000249:Adenosine Monophosphate / Q000378:metabolism / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D019343:Citric Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005635:Fructosediphosphates / Q000378:metabolism / Q000494:pharmacology; D005636:Fructosephosphates / Q000378:metabolism; D007672:Kidney Cortex / Q000201:enzymology*; D010428:Pentosephosphates / Q000378:metabolism* / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats
D005635:Fructosediphosphates; D005636:Fructosephosphates; D010428:Pentosephosphates; C050562:ribose-1,5-bisphosphate; D019343:Citric Acid; D000249:Adenosine Monophosphate; C027618:fructose-6-phosphate; C027652:fructose 2,6-diphosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1