Screening for coeliac disease in healthy blood donors at two immuno-transfusion centres in north-east Italy.
OBJECTIVE
In the past, the reported prevalence of coeliac disease ranged from 1:1000 to 1:4000, whereas recent studies using serological screening methods have found a significantly higher prevalence. The aim of this study was to investigate the prevalence of coeliac disease in healthy blood donors in a North-eastern region of Italy.


METHODS
A total of 4000 healthy blood donors were studied from two immunotransfusion centres.


METHODS
Serum IgA-antiendomysium antibodies were detected by indirect immunofluorescence using human umbilical cord vein sections, and positive sera were tested also on monkey oesophagus tissue. Intestinal biopsy was performed in all antiendomysium-positive subjects.


RESULTS
Ten out of 4000 sera screened were found to be antiendomysium positive on human umbilical cord vein. All positive patients had flat mucosa on intestinal biopsy. Five subjects had coeliac disease-related clinical features (2 had a history of gastrointestinal symptoms, 1 a family history of IDDM, 1 sideropenic anaemia, and 1 IgA deficiency). One of the ten serum, antiendomysium positive on human umbilical cord vein, was found to be negative when tested on monkey oesophagus.


CONCLUSIONS
These data confirm the high prevalence of undiagnosed silent coeliac disease in the healthy adult population. This is the first study where umbilical cord was used for screening coeliac disease in a large population. The human umbilical cord vein indirect immunofluorescence test is more specific for villous atrophy than conventional indirect immunofluorescence test on monkey oesophagus and is a reliable screening test for coeliac disease in an apparently healthy population.
Trevisiol
C
C
Department of Paediatrics, IRCCS Burlo Garofolo, Trieste, Italy. chtrevi@tin.it
Not
T
T
Berti
I
I
Buratti
E
E
Città
A
A
Neri
E
E
Torre
G
G
Martelossi
S
S
Tommasini
A
A
Alù
A
A
Barillari
G
G
Facchini
S
S
Ventura
A
A
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001771:Blood Banks; D001782:Blood Donors / Q000706:statistics & numerical data*; D002446:Celiac Disease / Q000175:diagnosis / Q000453:epidemiology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007558:Italy / Q000453:epidemiology; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D015995:Prevalence; D017678:Sex Distribution
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A