title: Cytoplasmic inclusions in leukocytes. An unusual manifestation of cryoglobulinemia.
abstract: Cryoglobulins are circulating immunoglobulins characterized by reversible, cold-induced precipitation. A variety of laboratory abnormalities, including hypocomplementemia, elevated erythrocyte sedimentation rate, rheumatoid factor activity, pseudoleukocytosis, and pseudothrombocytosis, are associated with cryoglobulinemia. Extracellular, faintly basophilic, amorphous deposits of cryoglobulins occasionally have been described in blood smears. In the present study, smears prepared from blood collected at room temperature from 6 patients with cryoglobulinemia exhibited neutrophil and, occasionally, monocyte inclusions containing clear, light pink, or faintly basophilic amorphous material. The inclusions were absent in smears from blood collected and maintained at 37 degrees C. Ultrastructural examination revealed that the material within the leukocyte inclusions was consistent with phagocytosed immunoglobulins. The identification of characteristic cytoplasmic inclusions in leukocytes may be an important clue in the early recognition of cryoglobulinemia.
authors.affiliation: Department of Pathology, University of Texas Southwestern Medical Center at Dallas 75235, USA.
authors.forename: A
authors.initials: A
authors.lastname: Maitra
authors.forename: P C
authors.initials: PC
authors.lastname: Ward
authors.forename: S H
authors.initials: SH
authors.lastname: Kroft
authors.forename: B S
authors.initials: BS
authors.lastname: Levinson
authors.forename: S
authors.initials: S
authors.lastname: Jamal
authors.forename: A J
authors.initials: AJ
authors.lastname: Fishleder
authors.forename: K M
authors.initials: KM
authors.lastname: Sendelbach
authors.forename: R W
authors.initials: RW
authors.lastname: McKenna
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016477:Artifacts; D003080:Cold Temperature; D003449:Cryoglobulinemia / Q000097:blood / Q000473:pathology*; D003450:Cryoglobulins / Q000648:ultrastructure; D005260:Female; D006801:Humans; D002479:Inclusion Bodies / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology* / Q000648:ultrastructure; D009504:Neutrophils / Q000473:pathology* / Q000648:ultrastructure; D013048:Specimen Handling
chemical_list: D003450:Cryoglobulins