Cloning and characterization of a regulatory gene of the SARP family and its flanking region from Streptomyces ambofaciens.
In a search for activators of secondary metabolism we isolated a 12.6-kb DNA fragment from a genomic library of Streptomyces ambofaciens NRRL 2240 (the spiramycin producer ). Sequencing of 6 kb of the cloned fragment revealed a cluster of four ORFs (ORF1-4) whose deduced products showed similarities to those of other genes involved in polyketide biosynthesis, including a pathway-specific regulatory gene of the SARP family. The results of insertional inactivation of some of the cloned genes clearly indicate that the isolated cluster does not code for spiramycin production, suggesting that some other polyketide compound might well be produced by this strain.
Culebras
E
E
Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autónoma de Madrid, Spain.
Martínez
E
E
Carnero
A
A
Malpartida
F
F
D000595:Amino Acid Sequence; D000880:Anthraquinones / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D004271:DNA, Fungal; C013390:actinorhodin; D000095622:Benzoisochromanequinones