Human erythrocyte polyamine levels after portal vein embolization.
OBJECTIVE
Polyamine levels in erythrocytes are related to liver regeneration and could be used as an index of liver regeneration after partial hepatectomy. We investigated liver regeneration after portal vein embolization according to the changes of erythrocyte polyamine levels.


METHODS
Levels of polyamines (putrescine, spermidine, and spermine) in erythrocytes were assayed by high-pressure liquid chromatography for 13 patients with hepatocellular carcinoma after portal vein embolization and 16 patients (8 from group reported earlier) after right bisegmentectomy of the liver for hepatocellular carcinoma. In the first group, embolization preceded surgery by 3 weeks.


RESULTS
The mean total polyamine level in erythrocytes and the levels of spermidine and spermine were significantly higher at day 7 after embolization, decreasing later. Spermidine and spermine increased by day 7 after partial hepatectomy, decreasing later. Their mean increase was smaller and more gradual when embolization was done before resection than without embolization.


CONCLUSIONS
Embolization causes regeneration of the non-embolized portion of the liver, and embolization before liver resection allows regenerative activities of the liver remaining after resection to be lower than without the embolization.
Tsukamoto
T
T
Second Department of Surgery, Osaka City University Medical School, Japan.
Kinoshita
H
H
Hirohashi
K
K
Kubo
S
S
Tanaka
H
H
Otani
S
S
D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000628:therapy*; D002851:Chromatography, High Pressure Liquid; D004621:Embolization, Therapeutic; D004912:Erythrocytes / Q000378:metabolism*; D006498:Hepatectomy; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000628:therapy*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011073:Polyamines / Q000378:metabolism*; D011169:Portal Vein; D011379:Prognosis; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine