title: Comparison of PCR detection of mecA with methicillin and oxacillin disc susceptibility testing in coagulase-negative staphylococci.
abstract: The provisional BSAC method for the detection of methicillin sensitivity in coagulase-negative staphylococci (CNS) requires incubation of isolates for 48 h and raises the problem of timely reporting of susceptibility data. The forthcoming withdrawal of methicillin raises another difficulty. We evaluated 42 clinically significant CNS blood culture isolates by PCR, methicillin and oxacillin disc testing and by using methicillin Etests. Our results suggest that, although oxacillin disc susceptibility testing is a reasonable first line step, optimal and timely detection of resistance or susceptibility may require a combination of phenotypic and genotypic methods.
authors.affiliation: Public Health and Clinical Microbiology Laboratory, Royal Victoria Infirmary, Queen Victoria Road, Newcastle upon Tyne NE1 4LP, UK. J.C.Graham@ncl.ac.uk
authors.forename: J C
authors.initials: JC
authors.lastname: Graham
authors.forename: O M
authors.initials: OM
authors.lastname: Murphy
authors.forename: D
authors.initials: D
authors.lastname: Stewart
authors.forename: A M
authors.initials: AM
authors.lastname: Kearns
authors.forename: A
authors.initials: A
authors.lastname: Galloway
authors.forename: R
authors.initials: R
authors.lastname: Freeman
mesh_terms: D003030:Coagulase / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010068:Oxacillin / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
chemical_list: D003030:Coagulase; D010406:Penicillins; D008712:Methicillin; D010068:Oxacillin