A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes.
A preparation technique has been developed for plants with small chromosomes, which produces large numbers of good-quality mitotic preparations. The technique employs a hydrochloric acid treatment to hydrolyse the cytoplasm, facilitating the subsequent removal of cytoplasmic debris. The evaporative force of a methanol-based fixative is exploited to disperse the cytoplasm and to deposit the chromosomes in a single optical plane. This technique permits detailed observations of chromosome morphology and karyotyping. The mitotic preparations are also suitable for the complex analysis associated with in-situ hybridization, as in studies of genome interaction in plant hybrids.
Andras
S C
SC
The Ecology-Genetics Department, Faculty of Biology-Geology, University Babes-Bolyai, Cluj-Napoca Romania.
Hartman
T P
TP
Marshall
J A
JA
Marchant
R
R
Power
J B
JB
Cocking
E C
EC
Davey
M R
MR
D002875:Chromosomes; D003593:Cytoplasm; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008938:Mitosis; D059828:Plant Cells; D010944:Plants / Q000235:genetics*