Involvement of carbon source and acetyl phosphate in the external-pH-dependent expression of porin genes in Escherichia coli.
The porin composition of the Escherichia coli cell envelope was analyzed during growth at different external pHs (pHo) as a function of the acetyl phosphate (AcP) level (DeltaackA pta or ackA mutant, pyruvate or glucose as the carbon source) in the presence or absence of EnvZ. Our results indicate that the AcP level is influenced by the pHo, leading to modulation of the amount of OmpR-P and subsequent pHo-dependent expression of ompF and ompC. We also propose the existence of a specific signal, independent of EnvZ and AcP, leading to OmpR phosphorylation in response to pyruvate.
Heyde
M
M
Unité de Microbiologie et Génétique, UMR CNRS 5577, Université Lyon I, F-69622 Villeurbanne Cedex, France.
Laloi
P
P
Portalier
R
R
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002455:Cell Division / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins; D009097:Multienzyme Complexes; D009154:Mutation; D010755:Organophosphates / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D018272:Porins / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D015398:Signal Transduction
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D009097:Multienzyme Complexes; C113724:OmpC protein; C109237:OmpF protein; D010755:Organophosphates; D018272:Porins; C061279:PhoR protein, Bacteria; C011632:acetyl phosphate; D002244:Carbon; D019289:Pyruvic Acid; D011494:Protein Kinases; C066232:CreC protein, E coli; C057843:envZ protein, E coli; D010749:Phosphoprotein Phosphatases; D005947:Glucose