title: On the domain structure and the polymerization state of the sendai virus P protein.
abstract: The phosphoproteins (P) of paramyxoviruses and rhabdoviruses are cofactors of the viral polymerase (L) and chaperones of soluble nucleoprotein preventing its polymerization and nonspecific binding to cellular RNA. The primary sequences of six paramyxovirus P proteins were compared, and although there was virtually no sequence similarity, there were two regions with similar secondary structure predictions in the C-terminal part of P: the predicted multimerization domain and the X-protein, the sequence that binds to N in the N:RNA template. The C-terminal part of the Sendai virus P protein, the multimerization domain including the binding site for the polymerase, and the X-protein were expressed in Escherichia coli. All three polypeptides folded with secondary structures similar to those predicted. The C-terminal part of P is a very elongated molecule with most of its length encompassing the multimerization domain. Both the multimerization domain and the C-terminal part of P were found to form tetramers, whereas the X-protein was monomeric.
authors.affiliation: ESRF, Grenoble cedex 9, 38043, France.
authors.forename: N
authors.initials: N
authors.lastname: Tarbouriech
authors.forename: J
authors.initials: J
authors.lastname: Curran
authors.forename: C
authors.initials: C
authors.lastname: Ebel
authors.forename: R W
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: W P
authors.initials: WP
authors.lastname: Burmeister
mesh_terms: D000595:Amino Acid Sequence; D001704:Biopolymers / Q000737:chemistry; D002942:Circular Dichroism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D014461:Ultracentrifugation; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D001704:Biopolymers; C055859:P protein, Sendai virus; D010750:Phosphoproteins; D014764:Viral Proteins