The HTR1 gene is a dominant negative mutant allele of MTH1 and blocks Snf3- and Rgt2-dependent glucose signaling in yeast.
Saccharomyces cerevisiae HTR1 mutants are severely impaired in the uptake of glucose. We have cloned dominant HTR1 mutant alleles and show that they encode mutant forms of the Mth1 protein. Mth1 is shown to be involved in carbon source-dependent regulation of its own, invertase and hexose transporter gene expression. The mutant forms block the transduction of the Snf3- and Rgt2-mediated glucose signals upstream of the Rgt1 transcriptional regulator.
Schulte
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F
Institut für Mikrobiologie, Heinrich-Heine-Universität, D-40225 Düsseldorf, Germany.
Wieczorke
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R
Hollenberg
C P
CP
Boles
E
E
D048868:Adaptor Proteins, Signal Transducing; D000483:Alleles; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000502:physiology*; D006026:Glycoside Hydrolases / Q000378:metabolism; D008565:Membrane Proteins / Q000502:physiology*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction / Q000235:genetics*; D043324:beta-Fructofuranosidase
D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; C449502:MPT5 protein, S cerevisiae; C118806:MTH1 protein, S cerevisiae; D008565:Membrane Proteins; D009004:Monosaccharide Transport Proteins; C112512:RGT2 protein, S cerevisiae; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; C104463:SNF3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005947:Glucose