Silver staining of selected mitoses by physical development.
A silver staining method using light-insensitive physical developer for electron microscopic study of selected mitoses is described. The G-banded mitoses are stained with silver by a physical development process that preserves the original G-banding pattern. Transferring the light microscopically selected mitoses to grids provides an examination by optional high resolution in an electron microscope. The physical developer makes the staining standardizable and reproducible, and the intensity of the staining can be controlled by varying the time of development.
Trixler
M
M
Department of Psychiatry, University Medical School, Pécs, Hungary.
D001399:Azure Stains; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods*; D002878:Chromosomes, Human, Pair 1 / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods; D008938:Mitosis; D016622:Silver Staining / Q000379:methods*
D001399:Azure Stains