title: Gene transfer into hypothalamic organotypic cultures using an adeno-associated virus vector.
abstract: Organotypic cultures of rat hypothalamic slice cultures were successfully transduced using adeno-associated viral vectors. Using nuclear-targeted Lac-Z as the reporter gene, transduction was found to be very effective, occurring in as high as 89% of a specific cell type, the oxytocin neurons, present in the cultured explants. These transduction levels were not accompanied by any deleterious effects in the cultured cells 7 days after transduction. Such an in vitro approach should be valuable for the study of cell-specific gene expression in neurons in the central nervous system for which there are no homologous (surrogate) cell lines.
authors.affiliation: Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Keir
authors.forename: S B
authors.initials: SB
authors.lastname: House
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Xiao
authors.forename: H
authors.initials: H
authors.lastname: Gainer
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003587:Cytomegalovirus / Q000235:genetics; D000229:Dependovirus; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D007031:Hypothalamus; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009924:Organ Culture Techniques; D010121:Oxytocin / Q000032:analysis; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D010121:Oxytocin; D001616:beta-Galactosidase