Site-directed insertion and insertion-deletion mutations in the Escherichia coli chromosome simplified.
A procedure to produce an exact chromosomal replica of an insertion or insertion-deletion mutation produced in vitro in a plasmid with a ColE 1 origin of replication is presented. This procedure uses a previously described property of recD mutations (Biek DP, Cohen SN. J. Bacteriol. 1986;167:594-603) and is limited by (1) the compatibility of the new mutation with recD; and (2) the presence of some Escherichia coli DNA flanking the mutation.
Smith-White
B
B
Biochemistry Department, Michigan State University, East Lansing 48824-1319, USA. smithwhi@pilot.msu.edu
D002876:Chromosomes, Bacterial / Q000235:genetics*; D003087:Colicins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D018741:Replication Origin; D017384:Sequence Deletion
D003087:Colicins; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli