Protein recovery from several paper types used to collect gingival crevicular fluid.
The purpose of this study was to compare the efficiency of protein elution from several types of gingival crevicular fluid (GCF) collection papers when the volume of the inoculated protein and the elution methods were constant. Various concentrations of bovine serum albumin (BSA) and 14C-BSA were placed onto strips of Whatman #1 [W1], Whatman 3 MM chromatographic [W3], Periopaper (ProFlow) [P] and Periopaper (Harco) [H], and recovered proteins measured following a non-optimized centrifugal elution technique. There were significant differences in % recovery of BSA and 14C-BSA from the papers, which was dependent on both the type of paper and the concentration of the inoculated protein; that is, proteins at the lowest concentrations were less efficiently eluted from GCF collection papers than those at higher concentrations. Equations for regression lines of elution efficiency were quadratic. Thus, our data suggest significant differences in the efficiency for elution of BSA from absorbent papers when the volume of the inoculated fluid and the elution technique were constant. Previous variable or conflicting experimental data between research groups may have resulted from incomplete elution of proteins from GCF collection papers, possibly due to entrapment within, or binding of GCF proteins to the paper.
Johnson
R B
RB
Department of Periodontics, University of Mississippi Medical Center, Jackson 39216-4505, USA.
Streckfus
C F
CF
Dai
X
X
Tucci
M A
MA
D000042:Absorption; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D005374:Filtration / Q000295:instrumentation; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D010209:Paper; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data
D011506:Proteins; D012710:Serum Albumin, Bovine