Investigation of a tetracycline-regulated phage display system.
A new vector (pGZ1) was developed for bacterial phage display of antibody fragments using a transcriptional regulation element with tight control. The tet(p/) degrees -based phasmid exhibits fully suppressed scFv background synthesis in the absence of inducer and is independent of glucose as a catabolite repressor. The vector is shown to be a useful alternative to commonly used lac(p/) degrees -regulated systems.
Zahn
G
G
Institut für Biochemie (Charité), Humboldt-Universität zu Berlin, Monbijoustr.2, D-10117 Berlin, Germany.
Skerra
A
A
Höhne
W
W
D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005822:Genetic Vectors; D005947:Glucose / Q000494:pharmacology; D007128:Immunoglobulin Fragments / Q000235:genetics; D007763:Lac Operon; D019151:Peptide Library; D013752:Tetracycline / Q000494:pharmacology*; D014764:Viral Proteins / Q000235:genetics
D007128:Immunoglobulin Fragments; D019151:Peptide Library; D014764:Viral Proteins; D013752:Tetracycline; D005947:Glucose