title: Import of precursor proteins into mitochondria from soybean tissues during development.
abstract: Characterisation of the amount of protein import of the alternative oxidase (AOX) and the F(A)d precursor proteins (previously shown to use different import pathways) into mitochondria from developing soybean tissues indicated that they displayed different patterns. Import of the AOX declined in both cotyledon and root mitochondria with increasing age, whereas the import of the F(A)d into cotyledon mitochondria remained high throughout the same period. Using primary leaf mitochondria, it was evident that import of AOX remained high while it declined in cotyledon and root mitochondria. The amount of import of the AOX into mitochondria from different tissues closely matched the amount of the Tom 20 receptor.
authors.affiliation: Department of Biochemistry, University of Western Australia, Nedlands 6907, Perth, W.A., Australia.
authors.forename: M W
authors.initials: MW
authors.lastname: Murcha
authors.forename: T
authors.initials: T
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Whelan
mesh_terms: D001693:Biological Transport, Active; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000378:metabolism; D018548:Cotyledon / Q000201:enzymology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology; D011498:Protein Precursors / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology*; D013997:Time Factors; D000099188:Alternative Oxidase
chemical_list: D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D011498:Protein Precursors; D006180:Proton-Translocating ATPases; D000099188:Alternative Oxidase