[Dependence of properties of polyclonal antibodies to (2'-5')-oligoadenylates on the method of hapten binding to an immunogen].
To elucidate the antibody-(2'-5')oligoadenylate relation to the mode of the hapten-immunogen conjugation, a new (2'-5')oligoadenylic acid trimer derivative containing a 2'-terminal N6-(5-carboxypentyl)adenosine and its 125I-labeled immunogenic conjugate were synthesized. The immunization with this conjugate and with a conjugate based on the 2',3'-O-[1-(2-carboxyethyl)]ethylidene derivative of the (2'-5')triadenylic acid gave antisera with different affinities toward modified (2'-5')oligonucleotides. Epitopes involved in the (2'-5')oligomer-binding to different antisera were found.
Kulak
T I
TI
Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus.
Kvasiuk
E I
EI
Sentiureva
S L
SL
Shuliakovskaia
S M
SM
Matiushevskaia
V P
VP
Makarenko
M V
MV
Mikhaĭlopulo
I A
IA
D000227:Adenine Nucleotides / Q000276:immunology*; D000906:Antibodies / Q000276:immunology*; D003429:Cross Reactions; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D009843:Oligoribonucleotides / Q000276:immunology*; D011863:Radioimmunoassay; D014614:Vaccines, Synthetic / Q000276:immunology*
D000227:Adenine Nucleotides; D000906:Antibodies; D006241:Haptens; D009843:Oligoribonucleotides; D014614:Vaccines, Synthetic; C023505:2',5'-oligoadenylate