title: Purification and characterization of glutathione transferases from the sea bass (Dicentrarchus labrax) liver.
abstract: Two forms of glutathione transferase were purified from liver cytosol of the sea bass (Dicentrarchus labrax) by GSH-Sepharose affinity chromatography followed by chromatofocusing. The major enzyme (DL-GST-6.7; 75% of total activity bound to the column) has a pI value of 6.7 and is composed of two subunits of apparent molecular mass 26.5 kDa. The minor enzyme (DL-GST-8.2; 25% of total activity bound to the column) has a pI value of 8.2 and is composed of two subunits of molecular mass 23.5 kDa. Both isoenzymes appear to have blocked N-terminal. The purified proteins were characterized with respect to substrate specificity, CD spectra, TNS binding properties (with 2-toluidinylnaphthalene 6-sulfonate), and immunological reactivity. Partial internal amino acid sequence was also determined for each isoenzyme. The results obtained suggest that DL-GST-6.7 and DL-GST8.2 are novel GSTs belonging, respectively, to theta and alpha classes.
authors.affiliation: Dipartimento di Scienze Biomediche, Università "G. D'Annunzio,", Chieti, 66100, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Angelucci
authors.forename: P
authors.initials: P
authors.lastname: Sacchetta
authors.forename: P
authors.initials: P
authors.lastname: Moio
authors.forename: S
authors.initials: S
authors.lastname: Melino
authors.forename: R
authors.initials: R
authors.lastname: Petruzzelli
authors.forename: P
authors.initials: P
authors.lastname: Gervasi
authors.forename: C
authors.initials: C
authors.lastname: Di Ilio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001492:Bass / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000737:chemistry* / Q000145:classification; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000737:chemistry; D008099:Liver / Q000201:enzymology*; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D009282:Naphthalenesulfonates / Q000378:metabolism; D011485:Protein Binding; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D009282:Naphthalenesulfonates; C009497:2-(4-toluidino)-6-naphthalenesulfonic acid; D005982:Glutathione Transferase