Activation of monoamine oxidase type-B by aluminum in rat brain homogenate.
Monoamine oxidase type B (MAO-B) activity is elevated in certain neurological diseases such as Alzheimer's and Parkinson's disease with respect to age-matched controls; the cause of this elevation is unknown. The documented accumulation of aluminum in certain neurodegenerative diseases prompted us to test the effect of Al3+ on the activity of MAO-B in rat brain homogenate. Results showed that the metal ion significantly increased MAO-B enzymatic activity in a dose-dependent manner, yielding a K(M) of 5.69 microM compared with 34.45 microM in the absence of the metal ion. The Vmax of 45.34 micromol/min was unchanged in the presence of the metal ion.
Zatta
P
P
CNR-Center on Metalloproteins, University of Padova, Italy.
Zambenedetti
P
P
Milanese
M
M
D000535:Aluminum / Q000494:pharmacology*; D017607:Aluminum Compounds / Q000494:pharmacology; D000544:Alzheimer Disease / Q000201:enzymology / Q000209:etiology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002457:Cell Extracts; D003641:Deamination / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D007773:Lactates / Q000494:pharmacology; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D010300:Parkinson Disease / Q000201:enzymology / Q000209:etiology; D010627:Phenethylamines / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019354:Sodium Lactate / Q000494:pharmacology; D013816:Thermodynamics
D017607:Aluminum Compounds; D002457:Cell Extracts; D007773:Lactates; D010627:Phenethylamines; D000535:Aluminum; D008995:Monoamine Oxidase; D019354:Sodium Lactate; C104223:aluminum lactate