The effects of extracellular matrix on cell attachment, proliferation and migration in a human lens epithelial cell line.
Lens capsule consists of several kinds of extracellular matrix (ECM) which may play an important role in cell attachment, migration and proliferation of lens epithelial cells as a basement membrane. We have investigated the effects of ECM on cell attachment, proliferation and migration in a human lens epithelial (HLE) cell line. The HLE cell line, SRA 01/04, which was transfected with large T-antigen of SV40 was cultured in the absence of serum. Culture plates were coated with human type IV collagen, laminin or fibronectin. The number of cells were counted at 30-180 min and 3, 5 and 7 days of culture. The rate of BrdU incorporation was measured to study the cell proliferation. The cell migration was measured 1, 3, 5 and 7 days after seeding cells. Integrins, the receptors of ECM, were also detected using antibodies for the cell membrane antigens (CD49b, CD49c, CD49e) by an immunohistochemical method. Although less than 10% of cells attached to the non-coated plate and 50-60% of cells attached to the ECM-coated plates, there was no difference of cell attachment among each ECM used. The cell attachment was almost complete during the first 30 min of culture. Cell proliferation was not enhanced, but cell survival was aided by culture on the ECM components for up to 7 days. The area of cell attachment enlarged on the ECM-coated plates, whereas no migration was observed on the non-coated plate. These data indicate that ECM is the essential factor for cell attachment and increases migration of HLE cells.
Oharazawa
H
H
Department of Ophthalmology, Nippon Medical School, Tokyo, Japan.
Ibaraki
N
N
Lin
L R
LR
Reddy
V N
VN
D015703:Antigens, CD / Q000032:analysis; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D004847:Epithelial Cells; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D039421:Integrin alpha2; D039422:Integrin alpha3; D039482:Integrin alpha5; D016023:Integrins / Q000032:analysis; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D008858:Microscopy, Phase-Contrast
D015703:Antigens, CD; D039421:Integrin alpha2; D039422:Integrin alpha3; D039482:Integrin alpha5; D016023:Integrins