A prime-boost immunization strategy with DNA and recombinant baculovirus-expressed protein enhances protective immunogenicity of glycoprotein D of equine herpesvirus 1 in naïve and infection-primed mice.
The immunogenicity and protective efficacy afforded by intramuscular inoculation of plasmid DNA encoding equine herpesvirus 1 (EHV-1) glycoprotein D (gD) followed by EHV-1 gD expressed by a recombinant baculovirus was assessed in a murine model of EHV-1 respiratory infection. Compared with mice inoculated with DNA or protein only, mice inoculated with the combination of gD DNA and protein had enhanced ELISA and neutralizing antibody titres to EHV-1 and had accelerated clearance of virus from lungs following challenge infection. The enhanced protective effects of this consecutive immunization were also evident in mice which had a previous infection with EHV-1 and had pre-existing antibodies. The T-helper 1 (Th1) type of immune response induced by EHV-1 gD DNA was maintained after the protein boost, despite the gD protein alone appearing to direct a Th2 response.
Ruitenberg
K M
KM
Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia.
Walker
C
C
Love
D N
DN
Wellington
J E
JE
Whalley
J M
JM
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D005260:Female; D006566:Herpesviridae Infections / Q000517:prevention & control*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D007117:Immunization, Secondary; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D017778:Vaccines, Combined / Q000276:immunology / Q000627:therapeutic use; D019444:Vaccines, DNA / Q000276:immunology* / Q000627:therapeutic use; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
D017778:Vaccines, Combined; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1