Changes of splenocyte IFN-gamma mRNA synthesis in rats infected with Paragonimus westermani.
Changes in the expression level of splenocyte IFN-gamma mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of IFN-gamma had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with P. westermani, the levels of IFN-gamma mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.
Cho
J K
JK
Department of Parasitology, College of Medicine, Korea University, Seoul, Korea.
Kwon
H S
HS
Joo
K H
KH
Lee
J S
JS
Cho
S W
SW
D000818:Animals; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D010237:Paragonimiasis / Q000276:immunology*; D010238:Paragonimus; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D012333:RNA, Messenger; D007371:Interferon-gamma