Antiherpetic activities of various protein bound polysaccharides isolated from Ganoderma lucidum.
To investigate antiherpetic substances from Ganoderma lucidum, various protein bound polysaccharides, GLhw, GLhw-01, GLhw-02, GLhw-03, were isolated by activity-guided isolation from water soluble substances of the carpophores. These substances were examined for their antiviral activities against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) by plaque reduction assay in vitro. Among them, the acidic protein bound polysaccharide, GLhw-02 of a brownish substance, exhibited the most potent antherpetic activity with 50% effective concentrations (EC50) of 300 approximately 520 microg/ml in Vero and HEp-2 cells, and its selectivity indices (SI) were more than 20. GLhw-02 was identified to consist mainly of polysaccharide (approximately 40.6%) and protein (approximately 7.80%) by anthrone test and Lowry-Folin test, and showed the usual molar ratio (C:H:O = 1:2:1) of carbohydrates by elemental analysis. These results suggest that GLhw-02 possesses the possibility of being developed from a new antiherpetic agent.
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College of Pharmacy, Chungbuk National University, Cheongju, South Korea.
Kim
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Lee
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Han
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D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D011485:Protein Binding; D020940:Reishi / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012995:Solubility; D014709:Vero Cells; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D011134:Polysaccharides; D000212:Acyclovir