title: Prognostic significance of autoantibodies against tropomyosin in patients with colorectal adenocarcinoma.
abstract: We examined, with an enzyme-linked immunoadsorbent assay (ELISA) method, the serum of 55 patients with Colon Adenocarcinoma (CA) for the presence of autoantibodies against tropomyosin (TMS), of IgM and IgG isotypes, before and 1 month after surgery. Twenty-six (26) patients with benign surgical diseases (BSD) (hernia or cholelithiasis) and 40 healthy volunteers were used as controls. Preoperatively, 20/55 (36.3%) of CA patients and 2/26 (7.7%) of BSD patients were positive for anti-TMS antibodies, while postoperatively, the positive samples were 22/55 (40%) and 2/26 (7.7%), respectively. The difference between the group of CA patients and the two control groups was statistically significant (p < 0.001). The presence of anti-TMS antibodies has been associated with better outcome of CA patients: 30 CA patients (30/55, 54.5%) had detectable anti-TMS antibodies either preoperatively or postoperatively and 25 CA patients (25/55, 45%) were completely negative in both occasions. In the first group of patients, four (4) recurrences were detected (4/30, 13.3%) while in the second group nine (9) recurrences were found (9/25, 36%). The difference between the two groups was statistically significant (p < 0.01). Anti-tropomyosin antibodies could be used as biological markers of prognosis in colon cancer patients.
authors.affiliation: 3rd Department of Medicine, Athens Medical School, Sotiria General Hospital, Greece. knsyrigos@usa.net
authors.forename: K N
authors.initials: KN
authors.lastname: Syrigos
authors.forename: A
authors.initials: A
authors.lastname: Charalampopoulos
authors.forename: K
authors.initials: K
authors.lastname: Pliarchopoulou
authors.forename: E I
authors.initials: EI
authors.lastname: Syrigou
authors.forename: M
authors.initials: M
authors.lastname: Avuzuklidou
authors.forename: A
authors.initials: A
authors.lastname: Manouras
authors.forename: M
authors.initials: M
authors.lastname: Leandros
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000276:immunology / Q000601:surgery; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000601:surgery; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D014335:Tropomyosin / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D014335:Tropomyosin