A 1-Mb PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors.
We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21. 3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.
Yang
Y
Y
Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, Stockholm, Sweden.
Kiss
H
H
Kost-Alimova
M
M
Kedra
D
D
Fransson
I
I
Seroussi
E
E
Li
J
J
Szeles
A
A
Kholodnyuk
I
I
Imreh
M P
MP
Fodor
K
K
Hadlaczky
G
G
Klein
G
G
Dumanski
J P
JP
Imreh
S
S
D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D020451:Contig Mapping / Q000379:methods; D005354:Fibrosarcoma / Q000235:genetics*; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016513:Mice, SCID / Q000235:genetics*; D008969:Molecular Sequence Data
D005819:Genetic Markers