Correction of the DNA sequence of the regB gene of Rhodobacter capsulatus with implications for the membrane topology of the sensor kinase regB.
We corrected the previously published sequence for the regB gene, which encodes a histidine sensor kinase in Rhodobacter capsulatus. The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains.
Chen
W
W
Institut für Mikrobiologie und Molekularbiologie, D-35392 Giessen, Germany.
Jäger
A
A
Klug
G
G
D000469:Alkaline Phosphatase; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002462:Cell Membrane / Q000201:enzymology; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D011494:Protein Kinases; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000235:genetics*
D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D011993:Recombinant Fusion Proteins; C091081:RegB protein, Rhodobacter; D011494:Protein Kinases; D018844:Cyclin-Dependent Kinases; D000469:Alkaline Phosphatase