Antiapoptotic activity of herpes simplex virus type 2: the role of US3 protein kinase gene.
In order to determine the ability of herpes simplex virus type 2 (HSV-2) to suppress apoptosis, we examined the effect of HSV-2 infection on apoptosis induced in HEp-2 cells by treatment with 1 M sorbitol. Although a wild-type strain of HSV-2 induced apoptosis in a significant fraction of the infected cells, HSV-2 could suppress sorbitol-induced apoptosis in a manner similar to that of herpes simplex virus type 1 (HSV-1), indicating that HSV-2, like HSV-1, has an antiapoptosis gene. Characterization of the cells infected with a US3-deletion mutant of HSV-2 revealed the necessity of a US3 gene in the antiapoptotic activity of this virus.
Hata
S
S
Department of Virology, School of Medicine, The University of Tokushima, Tokushima, 770-8503, Japan.
Koyama
A H
AH
Shiota
H
H
Adachi
A
A
Goshima
F
F
Nishiyama
Y
Y
D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005814:Genes, Viral / Q000235:genetics / Q000502:physiology*; D018258:Herpesvirus 2, Human / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion / Q000235:genetics; D013012:Sorbitol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014764:Viral Proteins
D014764:Viral Proteins; D013012:Sorbitol; D017346:Protein Serine-Threonine Kinases; C084395:US3 protein, Human herpesvirus 2