[In vitro drug susceptibility test of Plasmodium falciparum using a portable thermostat and CO2 gas generator].
We have recently developed a method of in vitro cultivation of P. falciparum using a portable incubator and AnaeroPack.CO2 (Onda et al.), we applied semi-microtechnique drug susceptibility tests to the culture method to evaluate the system using several P. falciparum strains or isolates of different susceptibilities to chloroquine (SGE-1, FCR-3, K-1, Patient 1 and 2). The new method gave comparable results to those shown by the standard test employing a modular incubator chamber with standard gas composition of 5% O2, 5% CO2 and 90% N2. Many useful data on the epidemiology of drug resistant malaria such as the emergence of multi-drug resistant isolates could be collected by applying this new method to the field survey.
Lin
Q
Q
Department of Parasitology, Gunma University School of Medicine.
Onda
T
T
Kano
S
S
Masuda
G
G
Suzuki
M
M
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002245:Carbon Dioxide; D002738:Chloroquine / Q000494:pharmacology*; D003470:Culture Media; D004351:Drug Resistance; D007185:Incubators; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development
D000962:Antimalarials; D003470:Culture Media; D002245:Carbon Dioxide; D002738:Chloroquine