title: [Molecular genetic analysis of hobo mobile genetic element polymorphism in the genome of Drosophila melanogaster line subjected to long-term selection].
abstract: The distribution of mobile genetic element hobo was examined in Drosophila melanogaster lines HA (high male mating activity) and LA (low male mating activity) before and after their isogenization using Southern blot hybridization. The probe containing a full-size hobo copy was shown to produce polymorphic multilocus hybridization with chromosomal DNA. The polymorphism was line-specific. A comparison of hybridization patterns in isogenic and original lines showed that isogenization in dysgenic crosses resulted in the appearance of additional hobo localization sites in LA but not in HA. The hobo destabilization in the LA genome correlated with genetic instability and the ability to induce H-E hybrid dysgenesis. The results obtained are discussed in relation to the possible role of hobo in inducing genetic variability in lines with low male mating activity, which may counteract deleterious consequences of inbreeding and selection in the negative direction.
authors.affiliation: Department of Genetics and Breeding, St. Petersburg State University, Russia.
authors.forename: E Sh
authors.initials: ESh
authors.lastname: Kuligina
authors.forename: E N
authors.initials: EN
authors.lastname: Imianitov
authors.forename: A F
authors.initials: AF
authors.lastname: Smirnov
authors.forename: L Z
authors.initials: LZ
authors.lastname: Kaĭdanov
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology; D008297:Male; D011110:Polymorphism, Genetic; D012641:Selection, Genetic
chemical_list: D004251:DNA Transposable Elements