Cleavage of porcine high molecular weight kininogen by the action of porcine pancreatic kallikrein.
Pancreatic kallikrein (KK), converts the single chain high molecular weight kininogen (HK) into a two-chain molecule accompanied by kinin release. Further degradation was not observed. Molecular weights of the heavy (H)-chain and light (L)-chain were estimated to be 61 kDa and 56 kDa, respectively, by SDS-PAGE. The amino (N)-terminal sequences of intact HK, reduced and carboxymethylated (RCM)-H-chain, and RCM-L-chain were determined. These results indicate that porcine pancreatic KK initially cleaved the Arg-Ser bond of HK, to form a two chain molecule. Then pancreatic KK cleaved the Met-Lys bond yielding kallidin and a kinin-free protein, which was apparently equal in size to the nicked kininogen.
Mashiko
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H
Division of Chemistry of Hygiene, Meiji College of Pharmacy, Kiyose, Tokyo, Japan.
Miyamoto
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K
Takahashi
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H
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D019679:Kininogen, High-Molecular-Weight / Q000378:metabolism*; D007705:Kinins / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D010446:Peptide Fragments / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013552:Swine; D020840:Tissue Kallikreins / Q000378:metabolism*
D019679:Kininogen, High-Molecular-Weight; D007705:Kinins; D010446:Peptide Fragments; D012967:Sodium Dodecyl Sulfate; D020840:Tissue Kallikreins