title: Distribution of the intermedilysin gene among the anginosus group streptococci and correlation between intermedilysin production and deep-seated infection with Streptococcus intermedius.
abstract: The distribution of intermedilysin, a human-specific cytolysin, among the anginosus group streptococci and the correlation of toxin production and infection by Streptococcus intermedius were investigated. PCR and Southern hybridization specific for the intermedilysin gene revealed that the toxin gene exists only in S. intermedius and no homologue to the toxin gene is distributed in S. anginosus and S. constellatus. Thus, the intermedilysin gene is useful as a marker gene of S. intermedius. Moreover, a human-specific hemolysis assay and Western blotting with intermedilysin-specific antibodies clearly demonstrated that the intermedilysin production level in isolates from deep-seated infections, such as brain and liver abscesses, is higher (6.2- to 10.2-fold, respectively) than in strains from normal habitats, such as dental plaque, or from peripheral infection sites. However, other candidate virulence factors of S. intermedius, such as chondroitin sulfate depolymerase, hyaluronidase, and sialidase activities, did not show such a clear correlation between enzymatic activity and isolation sites or disease severity. From these results, intermedilysin is likely to be the pathogenic or triggering factor of significance in inducing deep-seated infections with S. intermedius.
authors.affiliation: Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Tokushima 770-8506, Japan. nagammune@bio.tokushima-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Nagamune
authors.forename: R A
authors.initials: RA
authors.lastname: Whiley
authors.forename: T
authors.initials: T
authors.lastname: Goto
authors.forename: Y
authors.initials: Y
authors.lastname: Inai
authors.forename: T
authors.initials: T
authors.lastname: Maeda
authors.forename: J M
authors.initials: JM
authors.lastname: Hardie
authors.forename: H
authors.initials: H
authors.lastname: Kourai
mesh_terms: D015870:Gene Expression; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000032:analysis; D006801:Humans; D009055:Mouth / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D006026:Glycoside Hydrolases