title: Isolation and characterization of human and mouse ZIRTL, a member of the IRT1 family of transporters, mapping within the epidermal differentiation complex.
abstract: We report the precise mapping and characterization of ZIRTL (zinc-iron regulated transporter-like) gene, the first mammalian member of an extensive family of divalent metal ion transporters, comprising IRT1 and ZIP1, ZIP2, ZIP3, and ZIP4 in plants and ZRT1 and ZRT2 in yeast. The human gene maps at the telomeric end of the epidermal differentiation complex (EDC), within chromosomal band 1q21, while the mouse gene maps within the mouse EDC, on mouse chromosome 3, between S100A9 and S100A13. The structure of the human gene has been determined, and message was detected in most adult and fetal tissues including the epidermis. The mouse gene is developmentally regulated and found expressed in fetal and adult suprabasal epidermis, osteoblasts, small intestine, and salivary gland.
authors.affiliation: Division of Medical and Molecular Genetics, Department of Craniofacial Development, The Guy's, King's College and St. Thomas' Hospitals' Medical and Dental School, London, United Kingdom. m.lioumi@umds.ac.uk
authors.forename: M
authors.initials: M
authors.lastname: Lioumi
authors.forename: C A
authors.initials: CA
authors.lastname: Ferguson
authors.forename: P T
authors.initials: PT
authors.lastname: Sharpe
authors.forename: T
authors.initials: T
authors.lastname: Freeman
authors.forename: I
authors.initials: I
authors.lastname: Marenholz
authors.forename: D
authors.initials: D
authors.lastname: Mischke
authors.forename: C
authors.initials: C
authors.lastname: Heizmann
authors.forename: J
authors.initials: J
authors.lastname: Ragoussis
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D027682:Cation Transport Proteins; D002454:Cell Differentiation / Q000235:genetics; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns; D007501:Iron / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015032:Zinc / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D018076:DNA, Complementary; C403517:SLC39A1 protein, human; C485744:Slc39a1 protein, mouse; D007501:Iron; D015032:Zinc