title: A 1-Mb PAC contig spanning the common eliminated region 1 (CER1) in microcell hybrid-derived SCID tumors.
abstract: We have developed an elimination test to identify chromosomal regions that contain tumor inhibitory genes. Monochromosomal human/mouse microcell hybrids are generated and passaged through SCID mice. Derived tumors are then analyzed for deletions on the transgenomic chromosome. Using this strategy, we have previously identified a 1.6-cM common eliminated region 1 (CER1) on human 3p21. 3. We now report that CER1 contains 14 markers that are deleted in 19 SCID-derived tumors. A 1-Mb PAC contig that spans CER1 was assembled. Five chemokine receptor genes (CCR1, CCR3, CCR2, CCR5, and CCR6) were localized in CER1 in a 225-kb cluster. The lactotransferrin gene (LTF, or lactoferrin, LF), which reportedly has tumor inhibitory activity, also maps to CER1. Our results create a basis for characterization and further functional testing of genes within CER1.
authors.affiliation: Microbiology and Tumor Biology Center (MTC), Karolinska Institutet, Stockholm, Sweden.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Kiss
authors.forename: M
authors.initials: M
authors.lastname: Kost-Alimova
authors.forename: D
authors.initials: D
authors.lastname: Kedra
authors.forename: I
authors.initials: I
authors.lastname: Fransson
authors.forename: E
authors.initials: E
authors.lastname: Seroussi
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Szeles
authors.forename: I
authors.initials: I
authors.lastname: Kholodnyuk
authors.forename: M P
authors.initials: MP
authors.lastname: Imreh
authors.forename: K
authors.initials: K
authors.lastname: Fodor
authors.forename: G
authors.initials: G
authors.lastname: Hadlaczky
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: J P
authors.initials: JP
authors.lastname: Dumanski
authors.forename: S
authors.initials: S
authors.lastname: Imreh
mesh_terms: D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D020451:Contig Mapping / Q000379:methods; D005354:Fibrosarcoma / Q000235:genetics*; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D016513:Mice, SCID / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D005819:Genetic Markers