title: Echoviruses and coxsackie B viruses that use human decay-accelerating factor (DAF) as a receptor do not bind the rodent analogues of DAF.
abstract: Many serotypes of echovirus (EV) and Coxsackie B virus (CBV) bind human decay-accelerating factor (DAF) and use it as a receptor for infection. Analogues for DAF have been isolated from mice and rats and characterized; these analogues have amino acid identities to human DAF of approximately 60%. EV serotypes 3, 6', 7, 11-13, and 29 and CBV serotypes 1, 3, and 5 caused hemagglutination of human erythrocytes but not rat or mouse erythrocytes, suggesting failure to bind rodent DAF. To confirm this evidence, radiolabeled viruses were incubated with transfected Chinese hamster ovary (CHO) cells that were abundantly expressing each type of DAF. Only cells that expressed human DAF bound virus. Although binding of EV and CBV was specific for human DAF, complement inhibition by DAF expressed in CHO cells was similar for each analogue.
authors.affiliation: Complement Biology Group, Dept. of Medical Biochemistry, University of Wales College of Medicine, Heath Park, Cardiff, CF4 4XX, United Kingdom. SpillerB@cardiff.ac.uk
authors.forename: O B
authors.initials: OB
authors.lastname: Spiller
authors.forename: I G
authors.initials: IG
authors.lastname: Goodfellow
authors.forename: D J
authors.initials: DJ
authors.lastname: Evans
authors.forename: J W
authors.initials: JW
authors.lastname: Almond
authors.forename: B P
authors.initials: BP
authors.lastname: Morgan
mesh_terms: D000818:Animals; D018958:CD55 Antigens / Q000378:metabolism*; D003167:Complement Activation; D029822:Enterovirus B, Human / Q000378:metabolism*; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice; D051381:Rats; D011991:Receptors, Virus / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D018958:CD55 Antigens; D011991:Receptors, Virus