BOLD--a biological O-linked glycan database.
Glycans can be O-linked to proteins via the hydroxyl group of serine, threonine, tyrosine, hydroxylysine or hydroxyproline. Sometimes the glycan is O-linked to the hydroxyl group via a phosphodiester bond. The core monosaccharide residue may be N-acetylgalactosamine, N-acetylglucosamine, galactose, glucose, fucose, mannose, xylose or arabinose. These O-linked glycans can remain as a monosaccharide, but often a complex structure is built up by stepwise addition of monosaccharides. Monosaccharides known to be added include galactose, N-acetylglucosamine, fucose, N-acetylneuraminic acid, N-glycolylneuraminic acid and 2-keto-3-deoxynonulosonic acid. O-linked glycans can also contain sulfate and phosphate residues. This leads to the possibility of the existence of numerous O-glycan structures. The biological O-linked database (BOLD) is a relational database that contains information on O-linked glycan structures, their biological sources (with a link to the SWISS-PROT protein database), the references in which the glycan was described (with a link to MEDLINE), and the methods used to determine the glycan structure. The database provides a valuable resource for glycobiology researchers interested in O-linked oligosaccharide structures that have been previously described on proteins from different species and tissues.
Cooper
C A
CA
Department of Chemistry, Macquarie University, North Ryde, Australia. catherine.cooper@proteomesystems.com
Wilkins
M R
MR
Williams
K L
KL
Packer
N H
NH
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D016208:Databases, Factual; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*
D011134:Polysaccharides