Normal levels of serum glycoproteins maintained in beta-1, 4-galactosyltransferase I-knockout mice.
The galactose-mediated clearance of serum glycoproteins from the circulation was evaluated using beta-1,4-galactosyltransferase (beta-1,4-GalT) I-knockout mice. Partial structural study of the oligosaccharides released from mouse serum glycoproteins revealed that 77.4% of the oligosaccharides from beta-1,4-GalT I(+/+) mouse contain galactose, while 7.7% of those from beta-1,4-GalT I(-/-) mouse were galactosylated. Under the conditions, no significant change in serum protein concentrations was observed between the normal and mutant mice. The results indicate that the hepatic asialoglycoprotein receptor-mediated system is not functioning in the clearance of endogenous serum glycoproteins.
Kido
M
M
Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, Japan.
Asano
M
M
Iwakura
Y
Y
Ichinose
M
M
Miki
K
K
Furukawa
K
K
D000367:Age Factors; D000818:Animals; D015153:Blotting, Western; D005700:Galactosyltransferases / Q000235:genetics* / Q000502:physiology; D005838:Genotype; D006023:Glycoproteins / Q000097:blood*; D037102:Lectins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout
D006023:Glycoproteins; D037102:Lectins; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I