[The effect of in-situ nerve growth factor from different biological sources on the reinitiation of mouse oocyte meiotic maturation in culture and on parthenogenetic activation].
We studied the capacity of mouse oocytes to complete meiotic maturation in vitro and form the female pronucleus upon parthenogenetic activation by cycloheximide, in response to a single injection into the mouse ovaries in situ of a purified fraction of 2.5 S NGF from mouse submaxillary glands and beta-NGF from bovine sperm. Injection of NGF from both sources at 10 ng/ml with subsequent incubation of the ovaries for 1 h increased the capacity of matured oocytes for parthenogenetic formation of the pronucleus. The frequency of pronucleus formation in both "naked oocyte" and oocytes surrounded by the cumulus cells was four times that in the control.
Fedorushchenko
A N
AN
Institute of Biochemistry, Academy of Sciences of Uzbekistan, Tashkent.
Koval'
T Iu
TIu
Khamidov
D Kh
DKh
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D008297:Male; D008540:Meiosis / Q000187:drug effects*; D051379:Mice; D008808:Mice, Inbred CBA; D020932:Nerve Growth Factor / Q000302:isolation & purification / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D010312:Parthenogenesis / Q000187:drug effects*; D013094:Spermatozoa; D013363:Submandibular Gland
D020932:Nerve Growth Factor