Boldine action against the stannous chloride effect.
Peumus boldus extract has been used in popular medicine in the treatment of biliar litiase, hepatic insufficiency and liver congestion. Its effects are associated to the substance boldine that is present in its extract. In the present work, we evaluated the influence of boldine both in: (i) the structural conformation of a plasmid pUC 9.1 through gel electrophoresis analysis; and in (ii) the survival of the strain of Escherichia coli AB1157 submitted to reactive oxygen species (ROS), generated by a Fenton like reaction, induced by stannous chloride. Our results show a reduction of the lethal effect induced by stannous chloride on the survival of the E. coli culture in the presence of boldine. The supercoiled form of the plasmid is not modified by stannous chloride in the presence of boldine. We suggest that the protection induced by boldine could be explained by its anti-oxidant mechanism. In this way, the boldine could be reacting with stannous ions, protecting them against the oxidation and, consequently, avoiding the generation of ROS.
Reiniger
I W
IW
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofísica e Biometria, Brazil.
Ribeiro da Silva
C
C
Felzenszwalb
I
I
de Mattos
J C
JC
de Oliveira
J F
JF
da Silva Dantas
F J
FJ
Bezerra
R J
RJ
Caldeira-de-Araújo
A
A
Bernardo-Filho
M
M
D000975:Antioxidants / Q000494:pharmacology*; D001060:Aporphines / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000187:drug effects*; D008519:Medicine, Traditional; D018515:Plant Leaves / Q000737:chemistry; D010957:Plasmids / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013020:South America; D013997:Time Factors; D017971:Tin Compounds / Q000633:toxicity
D000975:Antioxidants; D001060:Aporphines; D017382:Reactive Oxygen Species; D017971:Tin Compounds; C023599:stannous chloride; C011686:boldine