title: PpdD type IV pilin of Escherichia coli K-12 can Be assembled into pili in Pseudomonas aeruginosa.
abstract: Escherichia coli K-12 possesses at least 16 chromosomal genes related to genes involved in the formation of type IV pili in other gram-negative bacteria. However, E. coli K-12 does not produce type IV pili when grown under standard laboratory conditions. The results of reverse transcription-PCR, operon fusion analysis, and immunoblotting demonstrated that several of the putative E. coli piliation genes are expressed at very low levels. Increasing the level of expression of the major pilin gene (ppdD) and the linked assembly genes hofB and hofC (homologues of the Pseudomonas aeruginosa type IV pilus assembly genes pilB and pilC) did not lead to pilus production. However, expression of the ppdD gene in P. aeruginosa led to assembly of PpdD into pili that were recognized by antibodies directed against the PpdD protein. Assembly of PpdD into pili in P. aeruginosa was dependent on the expression of the pilB and pilC genes and independent of expression of the P. aeruginosa pilin structural gene pilA.
authors.affiliation: Unité de Génétique Moléculaire (CNRS URA 1773), Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: N
authors.initials: N
authors.lastname: Sauvonnet
authors.forename: P
authors.initials: P
authors.lastname: Gounon
authors.forename: A P
authors.initials: AP
authors.lastname: Pugsley
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000737:chemistry*; D008854:Microscopy, Electron; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; C000592919:PpdD protein, E coli; D036781:Fimbriae Proteins