Detection of BCR/ABL gene rearrangement and the elimination of rearranged clone in chronic myelocytic leukemia patients.
Cytogenetic and molecular studies were performed on 20 interferon-alpha receiving Turkish chronic myelocytic leukemia patients. Four different restriction endonucleases and bcr-G probe were used for southern blot analysis to detect rearrangements of the bcr gene. The RT-PCR method was also applied to detect chimeric bcr/abl mRNA. Seventeen patients showed a chromosomal break within the 5.8 kb M-bcr region by southern blot analysis while three cases out of 20 have not shown any rearrangement. These three cases were further analysed by RT-PCR and they were also found to be carrying the Philadelphia translocation (Ph). However, in four years of follow-up this RT-PCR positivity has disappeared, which suggests an elimination of Ph clone with prolonged interferon-alpha treatment.
Eren
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E
Department of Biology, METU, Ankara, Turkey. emel_tetik@hotmail.com
Aytac
U
U
Tetik
E
E
Akman
O
O
Kansu
E
E
Gunduz
U
U
D000328:Adult; D015139:Blotting, Southern; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D002999:Clone Cells; D004334:Drug Administration Schedule; D005260:Female; D005500:Follow-Up Studies; D015321:Gene Rearrangement; D016313:Genes, abl / Q000235:genetics*; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D008297:Male; D015201:Meta-Analysis as Topic; D008875:Middle Aged; D010677:Philadelphia Chromosome; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013995:Time; D014178:Translocation, Genetic / Q000235:genetics*
D007370:Interferon Type I; D012333:RNA, Messenger; D011994:Recombinant Proteins