A spectroscopic and thermodynamic study of Taxol nucleic acid complexes.
The interactions of natural and synthetic polynucleotide double strands with the antitumor agent paclitaxel and the oncological product "Taxol for Injection Concentrate" (abbreviated as taxol) were examined in diluted aqueous solutions by thermal denaturation profiles (Tm), CD spectra and UV-absorption measurements. Furthermore, DNA-paclitaxel and -taxol complexes in condensed nucleic acid solutions were studied by differential scanning calorimetry. As polynucleotides alternating and homologous poly[d(AT)] and poly[d(GC)] and calf thymus DNA were used. The results point to stabilizing interactions of paclitaxel to AT nucleotides, whereas in the presence of GC base pairings no interaction took place. Thereby the interaction to homologous (dA).(dT)-tracts seems to be preferred.
Bischoff
G
G
Department of Biochemistry, Martin Luther University Halle-Wittenberg, Saale, Germany. bischoff@fu-berlin.de
Gromann
U
U
Lindau
S
S
Meister
W V
WV
Hoffmann
S
S
D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D006361:Heating; D009691:Nucleic Acid Denaturation; D017239:Paclitaxel / Q000737:chemistry*; D011067:Poly dA-dT / Q000737:chemistry*; D011089:Polydeoxyribonucleotides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; C019530:poly(dC-dG); D017239:Paclitaxel