title: Impaired tolerance to repetitive hypoxia in hippocampal slices of Cu,Zn superoxide dismutase transgenic mice.
abstract: Energy metabolism is impaired in the Cu,Zn superoxide dismutase transgenic mouse model of amyotrophic lateral sclerosis. The goal was to investigate tolerance against single and repetitive hypoxia in C57B6SJL-TgN(SOD1-G93A)1GUR mice (G93A mice). Posthypoxic recovery (15 min hypoxia, 45 min recovery) of population spike amplitude in hippocampal region CA1 was 38 +/- 29% (mean +/- SD) in controls and 67 +/- 41% (ns) in G93A mice at day 40. Upon in vivo pretreatment with 20 mg/kg 3-nitropropionate posthypoxic recovery increased to 82 +/- 32% (P < 0.01) in controls and decreased to 35 +/- 33% in G93A mice (P < 0.05 to pretreated controls). Results at day 80 and 110 were similar. We conclude that G93A mice show a long-lasting impairment to sustain repetitive hypoxic episodes whereas tolerance to a single hypoxic episode is comparable to controls.
authors.affiliation: Department of Neurology, University of Ulm, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Büchner
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: M
authors.initials: M
authors.lastname: Timmler
authors.forename: I
authors.initials: I
authors.lastname: Sehrsam
authors.forename: K
authors.initials: K
authors.lastname: Kasischke
authors.forename: H
authors.initials: H
authors.lastname: Völkel
authors.forename: A C
authors.initials: AC
authors.lastname: Ludolph
authors.forename: M W
authors.initials: MW
authors.lastname: Riepe
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism; D000818:Animals; D015687:Cell Hypoxia / Q000235:genetics*; D006624:Hippocampus / Q000502:physiology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013482:Superoxide Dismutase / Q000235:genetics*; D009435:Synaptic Transmission / Q000235:genetics*
chemical_list: D013482:Superoxide Dismutase