Mechanism of ubiquinol oxidation by the bc(1) complex: role of the iron sulfur protein and its mobility.
Native structures of ubihydroquinone:cytochrome c oxidoreductase (bc(1) complex) from different sources, and structures with inhibitors in place, show a 16-22 A displacement of the [2Fe-2S] cluster and the position of the C-terminal extrinsic domain of the iron sulfur protein. None of the structures shows a static configuration that would allow catalysis of all partial reactions of quinol oxidation. We have suggested that the different conformations reflect a movement of the subunit necessary for catalysis. The displacement from an interface with cytochrome c(1) in native crystals to an interface with cytochrome b is induced by stigmatellin or 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and involves ligand formation between His-161 of the [2Fe-2S] binding cluster and the inhibitor. The movement is a rotational displacement, so that the same conserved docking surface on the iron sulfur protein interacts with cytochrome c(1) and with cytochrome b. The mobile extrinsic domain retains essentially the same tertiary structure, and the anchoring N-terminal tail remains in the same position. The movement occurs through an extension of a helical segment in the short linking span. We report details of the protein structure for the two main configurations in the chicken heart mitochondrial complex and discuss insights into mechanism provided by the structures and by mutant strains in which the docking at the cytochrome b interface is impaired. The movement of the iron sulfur protein represents a novel mechanism of electron transfer, in which a tethered mobile head allows electron transfer through a distance without the entropic loss from free diffusion.
Crofts
A R
AR
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Guergova-Kuras
M
M
Huang
L
L
Kuras
R
R
Zhang
Z
Z
Berry
E A
EA
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D001665:Binding Sites; D002645:Chickens; D003198:Computer Simulation; D003461:Crystallography; D003573:Cytochrome b Group / Q000737:chemistry; D014450:Electron Transport Complex III / Q000737:chemistry* / Q000235:genetics; D004791:Enzyme Inhibitors / Q000737:chemistry; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics; D008024:Ligands; D008929:Mitochondria, Heart / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010084:Oxidation-Reduction; D011090:Polyenes / Q000737:chemistry; D015202:Protein Engineering; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013267:Stilbenes / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry
C025993:5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole; D000900:Anti-Bacterial Agents; D003573:Cytochrome b Group; D004791:Enzyme Inhibitors; D007506:Iron-Sulfur Proteins; D008024:Ligands; D011090:Polyenes; D013267:Stilbenes; D013844:Thiazoles; C056108:3-methoxy-2-(2-styrylphenyl)propenic acid methyl ester; D014451:Ubiquinone; C041573:stigmatellin; D014450:Electron Transport Complex III; C003741:ubiquinol