Shiga-toxin producing Escherichia coli from healthy cattle in a semi-urban community in Calcutta, India.
From October to December 1998, single faecal samples from 67 healthy cattle in a semi-urban community near Calcutta were examined for Shiga toxin producing Esch. coli (STEC) using a multiplex PCR primary screen followed by plating on sorbitol MacConkey agar. STEC was isolated from the faeces of 7 (10.5%) animals. The eight strains isolated belonged to eight serotypes viz, O146:H1, O149:HNT, ONT:H34, ONT:H19, O88:HN, ONT:H2, O82:H8 and O28ac:H21. Bead enzyme linked immunosorbent assay showed that three strains produced Shiga toxin 1, one produced Shiga toxin 2 and four produced both.
Pal
A
A
National Institute of Cholera & Enteric Diseases, Calcutta.
Ghosh
S
S
Ramamurthy
T
T
Yamasaki
S
S
Tsukamoto
T
T
Bhattacharya
S K
SK
Nair
G B
GB
Takeda
Y
Y
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle / Q000382:microbiology*; D004926:Escherichia coli / Q000378:metabolism*; D007194:India; D022603:Shiga Toxins
D001427:Bacterial Toxins; D022603:Shiga Toxins