Anti-feline immunodeficiency virus (FIV) soluble factor(s) produced from antigen-stimulated feline CD8(+) T lymphocytes suppresses FIV replication.
Feline immunodeficiency virus (FIV) causes AIDS-like symptoms in infected cats. Concanavalin A (ConA)-stimulated peripheral blood mononuclear cells (PBMC) from chronically FIV strain PPR-infected cats readily expressed FIV. In contrast, when PBMC from these animals were stimulated with irradiated, autologous antigen-presenting cells (APC), at least a 10-fold drop in viral production was observed. In addition to FIV-specific cytotoxic T lymphocytes, anti-FIV activity was demonstrated in the cell-free supernatants of effector T lymphocytes stimulated with APC. The FIV-suppressive activity was induced from APC-stimulated PBMC of either FIV-infected or uninfected cats but not from ConA-stimulated PBMC. Suppression of FIV strain PPR replication was observed for both autologous and heterologous feline PBMC, was dose dependent, and demonstrated cross-reactivity and cell specificity. It was also demonstrated that the anti-FIV activity originated from CD8(+) T lymphocytes and was mediated by a noncytolytic mechanism.
Choi
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IS
Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77843-4467, USA.
Hokanson
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R
Collisson
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D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D000998:Antiviral Agents / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002415:Cats; D002478:Cells, Cultured; D054407:Chemokine CCL4; D003208:Concanavalin A / Q000494:pharmacology; D003429:Cross Reactions; D003470:Culture Media; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000276:immunology* / Q000502:physiology; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000821:virology; D019402:Macrophage Inflammatory Proteins / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000821:virology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000998:Antiviral Agents; D054407:Chemokine CCL4; D003470:Culture Media; D019402:Macrophage Inflammatory Proteins; D008934:Mitogens; D003208:Concanavalin A