Substitution of amino acid residue in influenza A virus hemagglutinin affects recognition of sialyl-oligosaccharides containing N-glycolylneuraminic acid.
Sialic acids are essential components of cell surface receptors used by influenza viruses. To determine the molecular mechanisms of viral recognition of two major species of sialic acids, N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), we tested the binding reactivity of nine human H3 influenza A viruses to sialylglycolipids containing type II sugar chain and different molecular species of terminal sialic acids. All human H3 viruses tested except A/Memphis/1/71 bound both Neu5Ac and Neu5Gc. Nucleotide sequence analysis suggests that amino acids at 143, 155, and 158 are linked to the viral recognition of Neu5Gc.
Masuda
H
H
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka, Japan.
Suzuki
T
T
Sugiyama
Y
Y
Horiike
G
G
Murakami
K
K
Miyamoto
D
D
Jwa Hidari
K I
KI
Ito
T
T
Kida
H
H
Kiso
M
M
Fukunaga
K
K
Ohuchi
M
M
Toyoda
T
T
Ishihama
A
A
Kawaoka
Y
Y
Suzuki
Y
Y
D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D006001:Glycoconjugates / Q000378:metabolism; D006388:Hemagglutinins / Q000737:chemistry*; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012795:Sialoglycoproteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
D006001:Glycoconjugates; D006388:Hemagglutinins; D009438:Neuraminic Acids; D012795:Sialoglycoproteins; D014764:Viral Proteins; C032592:N-glycolylneuraminic acid