DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA.
Transcriptional regulation of Escherichia coli ATCC11105 penicillin amidase gene (pac) by cAMP receptor protein (CRP) and phenylacetic acid (PAA) was studied by using operon fusions with divergent reporter gene (lacZ, and phoA) constructs. A 150 bp DNA segment essential for the regulation of pac gene transcription by CRP and PAA was defined.
Radoja
S
S
Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia. radojs01@popmail.med.nyu.edu
Francetić
O
O
Stojićević
N
N
Morić
I
I
Glisin
V
V
Konstantinović
M
M
D001483:Base Sequence; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D010399:Penicillin Amidase / Q000235:genetics*; D010648:Phenylacetates / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D010648:Phenylacetates; D011993:Recombinant Fusion Proteins; D010399:Penicillin Amidase; C025136:phenylacetic acid