[Functional heterogeneity of polymorphonuclear leukocytes population in peripheral blood of patients with acute dysentery].
OBJECTIVE
To study chemiluminescence (CL) of polymorphonuclear neutrophils (PMNL) in peripheral blood of patients with acute mild and moderate dysentery caused by Shig. Flexneri in response to Re- and S-chemotype lipopolysaccharide (LPS) Sh. Flexneri and agonists fMLP, PMA and OZ.


RESULTS
PMNL population proved functionally heterogeneic, correlating with acute dysentery severity. Subgroups of PMNL varied by CL amplitude, reserve ability to additional stimulation. LPS rather modulated CL of PMNL than stimulated it. CL intensity depended on LPS structure and concentration. Mild acute dysentery was characterized by pool of primed PMNL which had higher functional reserves.


CONCLUSIONS
CL analysis can be used for raising accuracy of dysentery prognosis and severity, assessment of implications of functional state of neutrophil granulocytes for support of antiendotoxin immunity in acute dysentery.
Gorobchenko
A N
AN
Malov
V A
VA
Grachev
S V
SV
Pak
S G
SG
Roslyĭ
I M
IM
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D004405:Dysentery, Bacillary / Q000097:blood* / Q000175:diagnosis; D006801:Humans; D008070:Lipopolysaccharides / Q000819:agonists / Q000494:pharmacology; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D011379:Prognosis; D012720:Severity of Illness Index; D012763:Shigella flexneri / Q000378:metabolism / Q000472:pathogenicity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D008070:Lipopolysaccharides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate