Generating MHC class I ligands from viral gene products.
MHC class I molecules function to present peptides comprised of eight to 11 residues to CD8+ T lymphocytes. Here we review the efforts of our laboratory to understand how cells generate such peptides from viral gene products. We particularly focus on the nature of substrates acted on by cytosolic proteases, the contribution of proteasomes and non-proteasomal proteases to peptide generation, the involvement of ubiquitination in peptide generation, the intracellular localization of proteasome generation of antigenic peptides, and the trimming of peptides in the endoplasmic reticulum.
Yewdell
J
J
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892-0440, USA. jyewdell@nih.gov
Antón
L C
LC
Bacik
I
I
Schubert
U
U
Snyder
H L
HL
Bennink
J R
JR
D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000235:genetics* / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003546:Cysteine Endopeptidases / Q000378:metabolism; D003600:Cytosol / Q000276:immunology / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011499:Protein Processing, Post-Translational; D014452:Ubiquitins / Q000378:metabolism
D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D008024:Ligands; D009097:Multienzyme Complexes; D010455:Peptides; D014452:Ubiquitins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex