Physicochemical aspects of the movement of the rieske iron sulfur protein during quinol oxidation by the bc(1) complex from mitochondria and photosynthetic bacteria.
Crystallographic structures for the mitochondrial ubihydroquinone:cytochrome c oxidoreductase (bc(1) complex) from different sources, and with different inhibitors in cocrystals, have revealed that the extrinsic domain of the iron sulfur subunit is not fixed [Zhang, Z., Huang, L., Shulmeister, V. M., Chi, Y.-I., Kim, K. K., Hung, L.-W., Crofts, A. R., Berry, E. A., and Kim, S.-H. (1998) Nature (London), 392, 677-684], but moves between reaction domains on cytochrome c(1) and cytochrome b subunits. We have suggested that the movement is necessary for quinol oxidation at the Q(o) site of the complex. In this paper, we show that the electron-transfer reactions of the high-potential chain of the complex, including oxidation of the iron sulfur protein by cytochrome c(1) and the reactions by which oxidizing equivalents become available at the Q(o) site, are rapid compared to the rate-determining step. Activation energies of partial reactions that contribute to movement of the iron sulfur protein have been measured and shown to be lower than the high activation barrier associated with quinol oxidation. We conclude that the movement is not the source of the activation barrier. We estimate the occupancies of different positions for the iron sulfur protein from the crystallographic electron densities and discuss the parameters determining the binding of the iron sulfur protein in different configurations. The low activation barrier is consistent with a movement between these locations through a constrained diffusion. Apart from ligation in enzyme-substrate or inhibitor complexes, the binding forces in the native structure are likely to be < = RT, suggesting that the mobile head can explore the reaction interfaces through stochastic processes within the time scale indicated by kinetic measurements.
Crofts
A R
AR
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Hong
S
S
Zhang
Z
Z
Berry
E A
EA
D000818:Animals; D001665:Binding Sites; D003461:Crystallography; D003573:Cytochrome b Group / Q000737:chemistry; D003575:Cytochromes c1 / Q000737:chemistry; D014450:Electron Transport Complex III / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D007700:Kinetics; D010084:Oxidation-Reduction; D011487:Protein Conformation; D013696:Temperature; D013816:Thermodynamics; D013844:Thiazoles; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry
C025993:5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole; D003573:Cytochrome b Group; D007506:Iron-Sulfur Proteins; C037989:Rieske iron-sulfur protein; D013844:Thiazoles; D014451:Ubiquinone; D003575:Cytochromes c1; D014450:Electron Transport Complex III; C003741:ubiquinol