Viability of Lactobacillus gasseri and its cholesterol-binding and antimutagenic activities during subsequent refrigerated storage in nonfermented milk.
The effect storage at 4 degrees C on the viability of Lactobacillus gasseri and its sodium taurocholate-deconjugating and cholesterol-binding abilities as well as desmutagenic activity was investigated. Unfermented milks containing L. gasseri strains SBT0274 and SBT0270 at 10(9) cfu/ml were prepared using 10% skim milk. Total and bile-tolerant lactobacilli for strains SBT0274 and SBT0270 generally decreased after 14 d of storage at 4 degrees C; however, viable cells of these strains were still at 10(8) cfu/ml after 28 d of storage. The amounts of cholic acid released and of cholesterol bound by strains SBT0274 and SBT0270 declined over time, especially at 21 d of storage. Antimutagenic activity of unfermented milk made from both strains was attributed to the bacterial cells, and the activity was stable during storage at 4 degrees C for 28 d.
Usman
Faculty of Agriculture, Shinshu University, Nagano-ken, Japan.
Hosono
A
A
D000818:Animals; D016587:Antimutagenic Agents; D002784:Cholesterol / Q000378:metabolism*; D003080:Cold Temperature; D015169:Colony Count, Microbial; D005285:Fermentation; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism; D008892:Milk / Q000382:microbiology*; D009152:Mutagenicity Tests; D013656:Taurocholic Acid / Q000378:metabolism; D013997:Time Factors
D016587:Antimutagenic Agents; D013656:Taurocholic Acid; D002784:Cholesterol