Crystallinity of lyophilised carrot cell wall components.
The aim of this work was to investigate the effect of removal of cell wall components on the crystallinity of cell walls using X-ray diffraction. Various insoluble cell wall residues were prepared following a sequential extraction of carrot cell wall material. X-ray diffraction patterns were typical of cellulose although there was a possible contribution of pectic polysaccharides to the crystallinity. As more amorphous material was removed to produce a cellulose rich residue, the crystallinity index increased from 12 to 16%, larger than that estimated from cellulose alone. For the last residue treated with 4M KOH, a lower value of crystallinity was found (14%) which resulted from the change of some crystalline domains of cellulose into amorphous regions. Pressing conditions (temperature, water content) have been investigated and did not alter the crystallinity index significantly.
Georget
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DM
Norwich Laboratory, Institute of Food Research, Norwich Research Park, Colney, UK.
Cairns
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Smith
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Waldron
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KW
D001894:Borohydrides; D002021:Buffers; D002254:Carbonates; D002473:Cell Wall / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D018552:Daucus carota / Q000737:chemistry* / Q000648:ultrastructure; D004492:Edetic Acid / Q000031:analogs & derivatives; D006878:Hydroxides; D019800:Phenol; D011134:Polysaccharides / Q000737:chemistry; D017680:Potassium Compounds; D012967:Sodium Dodecyl Sulfate; D014961:X-Ray Diffraction
D001894:Borohydrides; D002021:Buffers; D002254:Carbonates; D006878:Hydroxides; D011134:Polysaccharides; D017680:Potassium Compounds; D019800:Phenol; D012967:Sodium Dodecyl Sulfate; C005686:sodium carbonate; C005390:CDTA; C025364:sodium borohydride; D002482:Cellulose; D004492:Edetic Acid; C029943:potassium hydroxide