Human polymorphonuclear neutrophils express a B7-1-like molecule.
Polymorphonuclear neutrophils (PMN) are part of the innate immune system and are first-line effector cells in acute inflammatory responses. On activation PMNs secrete cytokines and oxygen metabolites that might be involved in the regulation of the acquired immune response. We show here that peripheral blood PMNs constitutively express a B7-1-like molecule as detected by immunostaining with several B7-1 antibodies. Reverse transcriptase-polymerase chain reaction using three sets of primers spanning different regions of B7-1 indicate dissimilarities at the mRNA level. B7-1 mRNA is expressed in bone marrow cells and lipopolysaccharide (LPS)-stimulated but not in unstimulated PMNs. The B7-1-like molecule is localized to the cytoplasmic granules and translocated to the cell surface after stimulation with LPS or interleukin-12 in some donors. Binding of CTLA4-Ig suggests that the B7-1-like molecule can interact with functional B7 ligand and might be important in the immunobiology of PMNs.
Windhagen
A
A
Department of Neurology, Medical School Hannover, Germany. Windhagen.Anja@MHHannover.de
Maniak
S
S
Gebert
A
A
Ferger
I
I
Wurster
U
U
Heidenreich
F
F
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics; D000954:Antigens, Surface / Q000096:biosynthesis; D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics; D051940:B7-2 Antigen; D002478:Cells, Cultured; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics; D016920:Meningitis, Bacterial / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology / Q000378:metabolism; D018375:Neutrophil Activation / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D008562:Membrane Glycoproteins; D012333:RNA, Messenger