Method for intracellular magnetic labeling of human mononuclear cells using approved iron contrast agents.
A method for intracellular iron labeling of human mononuclear cells (lymphocytes and monocytes) for magnetic resonance imaging (MRI) using simple incubation of cells with approved MRI iron contrast agents is presented. Labeled cells can be detected by MRI in vitro, and this suggests the possibility that the technique could become a marker for in vivo lymphocyte and monocyte trafficking studies in acute inflammatory lesions such as those in Multiple Sclerosis.
Sipe
J C
JC
Neuroimaging Research Unit, San Raffaele Hospital, Milan, Italy. sipe.jack@hsr.it
Filippi
M
M
Martino
G
G
Furlan
R
R
Rocca
M A
MA
Rovaris
M
M
Bergami
A
A
Zyroff
J
J
Scotti
G
G
Comi
G
G
D002470:Cell Survival; D002478:Cells, Cultured; D003287:Contrast Media; D003911:Dextrans; D052203:Ferrosoferric Oxide; D006801:Humans; D007424:Intracellular Fluid; D007501:Iron; D008214:Lymphocytes / Q000473:pathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D009000:Monocytes / Q000473:pathology*; D009103:Multiple Sclerosis / Q000175:diagnosis*; D010087:Oxides; D015203:Reproducibility of Results; D013535:Suspensions
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D013535:Suspensions; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide