title: Optimisation of ion trap parameters for the quantification of chlormequat by liquid chromatography/mass spectrometry and the application in the analysis of pear extracts.
abstract: Optimisation of the activation parameters for ion trap mass spectrometric analysis of the chlormequat cation using simplex optimisation enabled the product ion (m/z 58) response to be improved 1000-fold. A comparison of the sensitivity of the optimised ion trap mass spectrometer with that of a triple quadrupole mass spectrometer for liquid chromatography/tandem mass spectrometry (LC/MS/MS) showed that similar limits of detection (LODs) could be achieved. For the MS/MS transition of the (35)Cl precursor to the most abundant product, LODs were 0.8 ng cation mL(-1) (0.004 mg cation kg(-1) pear equivalent) and 1.0 ng cation mL(-1) (0.005 mg cation kg(-1) pear equivalent) on the triple quadrupole and ion trap instrument, respectively.
authors.affiliation: Chemistry Department, University of York, Heslington, York YO10 5DD, UK.
authors.forename: C S
authors.initials: CS
authors.lastname: Evans
authors.forename: J R
authors.initials: JR
authors.lastname: Startin
authors.forename: D M
authors.initials: DM
authors.lastname: Goodall
authors.forename: B J
authors.initials: BJ
authors.lastname: Keely
mesh_terms: D002716:Chlormequat / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D010936:Plant Extracts; D002716:Chlormequat