Tick-borne encephalitis virus NS3 gene expression does not protect mice from homologous viral challenge.
Tick-borne encephalitis virus (TBEV) NS3 gene has been subcloned into the expression vector pcDNAI and expressed in eukaryotic cells. Immunization of mice with the recombinant plasmid pcDNAI-NS3 induced antibodies against NS3 protein but did not protect from viral challenge.
Morozova
O V
OV
Novosibirsk Institute of Bioorganic Chemistry, Novosibirsk, Russia. MOV@niboch.nsc.ru
Maksimova
T G
TG
Bakhvalova
V N
VN
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D002460:Cell Line; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D004675:Encephalitis, Tick-Borne / Q000517:prevention & control* / Q000821:virology; D007668:Kidney / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D020365:RNA Helicases; D012697:Serine Endopeptidases; D013552:Swine; D014162:Transfection; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000914:Antibodies, Viral; D020365:RNA Helicases; D012697:Serine Endopeptidases; D019444:Vaccines, DNA; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases