In vivo survival rate of rabbit morulae after vitrification in a medium without serum protein.
The in vivo survival rate of rabbit morulae after vitrification in a mixture of dimethyl sulphoxide and ethylene glycol solution without protein supplement (WPS) was compared with two types of protein supplements: rabbit serum (RS) and bovine serum albumin (BSA). Significant differences were observed in the percentage of transferable embryos (undamaged embryos after devitrification, 80.4% versus 93.2 and 92.1%, WPS, BSA and RS, respectively, P < 0.05) and live born rate (40.9% versus 56.1%, WPS and BSA, respectively, P < 0.05). Non-significant differences were, however, observed in the percentages of implanted embryos at 12 days post-ovulation induction (56.7, 69.7 and 68.6%), post-implantation survival rate (82.3, 74.2 and 77.2%) and live born rate in pregnant does (54.6, 56.1 and 50.5%) with different vitrification media (RS, BSA and WPS). We conclude that rabbit embryos can be vitrified and stored using protein-free vitrification medium with moderate losses of viability.
Vicente
J S
JS
Biotechnology Reproduction Laboratory, Politechnical University of Valencia, Spain. JVicent@dca.upv.es
Viudes-de-Castro
M P
MP
García
M L
ML
D000818:Animals; D001769:Blood; D001798:Blood Proteins; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D010064:Embryo Implantation; D004624:Embryo Transfer; D019855:Ethylene Glycol; D005260:Female; D005313:Fetal Death; D009028:Morula / Q000502:physiology*; D011247:Pregnancy; D011817:Rabbits; D012710:Serum Albumin, Bovine
D001798:Blood Proteins; D003451:Cryoprotective Agents; D012710:Serum Albumin, Bovine; D019855:Ethylene Glycol; D004121:Dimethyl Sulfoxide