[Human herpesvirus 6 A enhances HIV-1 replication in vitro by soluble mediators, this effect is diminished by endotoxin].
Simultaneous HHV-6A infection can activate HIV-1 latency and promote AIDS progression, but in this process the effects of HHV-6A induced soluble mediators on HIV-1 have not been studied yet. Recently, supernatants of HSB-2 cultures infected with HHV-6A and/or treated with endotoxin have been filtered virus free at time intervals until the cytopathic effect developed. Biological activity of some cytokines which might participate in HIV-1 activation was quantitated. Filtered supernatants were mixed into CEM-ss cultures, which had been HIV-1 infected at 1:1 cell:virus ratio, subsequently HIV-1 replication was quantitated and compared to controls. Supernatants filtered during the first 96 hours of HHV-6A replication without visible cytopathic effect augmented HIV-1 syncytium formation by tenfold, reverse transcriptase activity by threefold, p24 antigen production by 6-fold. Filtered supernatants obtained at onset of HHV-6A cytopathic effect did not modify HIV-1 replication. HSB-2 cultures produced no IL-2, and IFN-gamma induced by endotoxin diminished HIV-1 replication. HHV-6A delayed IFN-gamma release. An increase in the tumour necrosis factor activity upon the effect of HHV-6A and endotoxin was not parallel to HIV-1 activation. The putative mediator, different from those above which characterisation is in progress, might transmit similar transactivating effects between immune cells of lymph nodes and circulation.
Ongrádi
J
J
Pisai Egyetem, Orvosbiológiai Tanszék, Olaszország.
Ceccherini-Nelli
L
L
Matteucci
D
D
Bertók
L
L
Bendinelli
M
M
D004731:Endotoxins / Q000494:pharmacology*; D015497:HIV-1; D015654:Herpesvirus 6, Human; D006801:Humans; D066298:In Vitro Techniques; D012995:Solubility
D004731:Endotoxins