title: Effects of total parenteral nutrition on rat bile canaliculi: a stereologic analysis.
abstract: OBJECTIVE
  To quantitate, in a stereologic manner, changes in bile canalicular morphology before and after choleretic infusion of total parenteral nutrition (TPN) and to determine whether TPN produces changes in localized regions within the hepatic lobule.
  
  
  METHODS
  Livers were obtained from sham-operated on normal adult male rats (control) and from rats that received intravenous TPN solution containing 20% glucose and 3.5% Molipron F. The tissues, obtained by a rigorous sampling procedure, were systematically subjected to stereologic analysis. Measurements were made on electron micrographs at two levels of magnification by point, intersection and profile counts, and then volume, surface area and length were estimated per unit parenchymal volume.
  
  
  RESULTS
  The surface area of the canalicular wall per parenchymal volume increased significantly (from 5.33 x 10(-2) to 6.73 x 10(-2) microns 2/micron 3) after TPN treatment, as did the length of microvilli (from 0.241 to 0.267 microns/micron 3). However, the volume of bile canalicular lumina per parenchymal volume (0.306% and 0.320%), total length of bile canaliculi (1.05 x 10(-2) and 1.06 x 10(-2) microns/micron 3) and diameter of microvilli (8.73 x 10(-2) and 8.94 x 10(-2) microns) remained constant.
  
  
  CONCLUSIONS
  These results indicate that changes in canalicular shape and microvillus hypertrophy may cause lowering efficiency of the bile flow rate.
authors.affiliation: Institute for Medical Science, Dokkyo University School of Medicine, Tochigi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Kosaku
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D001648:Bile Canaliculi / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D007262:Infusions, Intravenous; D008297:Male; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D010289:Parenteral Nutrition, Total / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012996:Solutions
chemical_list: D012996:Solutions