title: Determination of substances of abuse in biological fluids by GC/MS using chemical ionization with acetonitrile.
abstract: GC/ms is an important method to determine drugs in biological fluids. For each of the most commonly used drugs a methanolic solution (100 micrograms/ml) was prepared; 30 microliters of each solution was evaporated using nitrogen flow and than diluted in 100 microliters of a derivatizing mixture constituted by MSTFA and toluene (30/70). After a short reaction time at room temperature 1 ml of each solution was injected into a SE-52 column (15 m x 0.25 mm) using chemical ionization with acetonitrile. This method has a good signal/noise ratio and is very useful in screening techniques.
authors.affiliation: Centro antidoping-Jockey Club Italiano, Milano.
authors.forename: C
authors.initials: C
authors.lastname: Mariani
authors.forename: G
authors.initials: G
authors.lastname: Crespi
authors.forename: M
authors.initials: M
authors.lastname: Montana
authors.forename: E
authors.initials: E
authors.lastname: Pini
authors.forename: R
authors.initials: R
authors.lastname: Stradi
mesh_terms: D000097:Acetonitriles; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D015813:Substance Abuse Detection / Q000295:instrumentation / Q000379:methods*
chemical_list: D000097:Acetonitriles; D007202:Indicators and Reagents; C032159:acetonitrile