Quantitative Assessment of Protein Adsorption by Combination of the Enzyme-Linked Immunosorbent Assay with Radioisotope-Based Studies.
Protein adsorption at polymer surfaces has been investigated by means of both ELISA and radiolabeling techniques. Most of the data obtained are linearly related to each other for protein concentrations between 0.01 and 1 µg/ml, i.e., the concentration range in which the maximum amount of adsorbed active protein (ELISA) is achieved. The correlation of ELISA data with radioisotope-based measurements allows quantification of the former. Specific correlation factors are described. Adsorption is shown to be strongly dependent on the polymer/protein system. Copyright 1999 Academic Press.
Balcells
M
Department of Textile Chemistry and Macromolecular Chemistry, RWTH Aachen, Veltmanplatz 8, Aachen, D-52062, Germany
Klee
D
Fabry
M
Höcker
H