title: Differential expression of nm23 H-1 protein in conjunctival melanoma and potential precursor lesions.
abstract: Loss of nm23 gene expression is believed to enhance metastatic spread in diverse human tumors, including skin melanoma. The purpose of this work was to determine the pattern and prognostic relevance of nm23 protein immunoexpression in conjunctival melanoma and potential precursor lesion. Formaldehyde-fixed, paraffin-embedded conjunctival specimens comprising 85 melanocytic lesions (nevi, primary aquired melanosis with and without atypia and primary and locally recurrent malignant melanomas) from 73 patients were used. Sections from all specimens were examined by light microscopy to assess diverse prognostic parameters. Additional sections were then immunostained for nm23 H-1 protein and the immunoreactivity was assessed semi-quantitatively. Survival data for all patients were retrieved from the National Causes of Death Registry of Sweden.Nm23 H-1 protein was differentially expressed in conjunctival melanocytic lesions, however loss of immunoexpression was not more common in melanocytic lesions asociated with a high risk of malignant transformation. Also, primary and recurrent conjunctival melanomas showed an essentially similar nm23 expression pattern and we could not associate the pattern of nm23 immunoexpression with an increased risk for malignant transformation or locally recurrent disease. While there was a tentative separation between cause-specific survival curves after excision for low and high nm23 expression conjunctival melanoma, there was no statistically significant association with metastatic death of patients. However, loss of nm23 protein immunoexpression may still be of some importance as a marker for prognosis in conjunctival melanoma because the present study could only detect large differences in survival. Our results suggest that any potential prognostic value of nm23 immunoexpression would be independent of other markers, underlining the importance of further studies.
authors.affiliation: Ophthalmic Pathology and Oncology Service, St Eriks Eye Hospital, Polhemsgatan 50, Stockholm, SE-112 82, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Seregard
authors.forename: M
authors.initials: M
authors.lastname: Oskarsson
authors.forename: B
authors.initials: B
authors.lastname: Spângberg
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D016009:Chi-Square Distribution; D003230:Conjunctival Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality; D008548:Melanosis / Q000378:metabolism / Q000401:mortality; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D054778:NM23 Nucleoside Diphosphate Kinases; D009364:Neoplasm Recurrence, Local / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality; D009508:Nevus, Pigmented / Q000737:chemistry / Q000378:metabolism / Q000401:mortality; D009701:Nucleoside-Diphosphate Kinase; D011230:Precancerous Conditions / Q000737:chemistry / Q000378:metabolism* / Q000401:mortality; D011379:Prognosis; D013194:Staining and Labeling; D016019:Survival Analysis; D014157:Transcription Factors / Q000032:analysis / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins