Extensive conservation of sex chromosome organization between cat and human revealed by parallel radiation hybrid mapping.
A radiation hybrid (RH)-derived physical map of 25 markers on the feline X chromosome (including 19 Type I coding loci and 6 Type II microsatellite markers) was compared to homologous marker order on the human and mouse X chromosome maps. Complete conservation of synteny and marker order was observed between feline and human X chromosomes, whereas the same markers identified a minimum of seven rearranged syntenic segments between mouse and cat/human X chromosome marker order. Within the blocks, the feline, human, and mouse marker order was strongly conserved. Similarly, Y chromosome locus order was remarkably conserved between cat and human Y chromosomes, with only one marker (SMCY) position rearranged between the species. Tight linkage and a conserved gene order for a segment encoding three genes, DFFRY-DBY-UTY in human, mouse, and cat Y chromosomes, coupled with demonstrated deletion effects of these genes on reproductive impairment in both human and mouse, implicates the region as critical for Y-mediated sperm production.
Murphy
W J
WJ
Laboratory of Genomic Diversity, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201,USA. murphywi@mail.ncifcrf.gov
Sun
S
S
Chen
Z Q
ZQ
Pecon-Slattery
J
J
O'Brien
S J
SJ
D000818:Animals; D001483:Base Sequence; D002415:Cats; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D005819:Genetic Markers