Changes in afferent impulse activity of small intestine mesenteric nerves in response to antigen challenge.
Sprague-Dawley rats (weight 130-150 g) were sensitized by an intraperitoneal injection of 1 mg chicken egg albumin with 0.25 ml Freund's adjuvant to stimulate immunoglobulin E antibody production. Leukocyte migration inhibitory factor was used as an indicator of animal sensitization. In acute electrophysiological experiments on sensitized animals, an intra-arterial or intraluminal chicken egg albumin (100 microg) challenge evoked a 10% enhancement of the activity of mesenteric nerves of the small intestine, regardless of the injection site chosen. Afferent nerve activity in control animals was not changed during the chicken egg albumin challenge. Morphometry at the light microscope level showed activation of mast cell degranulation after the antigen challenge to presensitized rats. Intraluminal injections of a stimulator of mast cell degranulation, compound 48/80 (20-30 mg), were found to increase afferent discharges in intact rats. An antagonist of H1 histamine receptors, clemastine, reduced the effect of compound 48/80. The results obtained provide direct evidence for the stimulation of sensory nerve endings by mast cell mediators released during mast cell degranulation.
Nozdrachev
A D
AD
Laboratory of Physiology of Sensory Receptors, Pavlov Institute of Physiology, Russian Academy of Sciences, St Petersburg.
Akoev
G N
GN
Filippova
L V
LV
Sherman
N O
NO
Lioudyno
M I
MI
Makarov
F N
FN
D000344:Afferent Pathways / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D000818:Animals; D000941:Antigens / Q000276:immunology / Q000494:pharmacology; D002452:Cell Count; D015550:Cell Degranulation; D002974:Clemastine / Q000494:pharmacology; D005260:Female; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007421:Intestine, Small / Q000294:innervation*; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008643:Mesentery / Q000294:innervation*; D009424:Nervous System Physiological Phenomena / Q000187:drug effects*; D010047:Ovalbumin / Q000276:immunology* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D003189:p-Methoxy-N-methylphenethylamine / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000941:Antigens; D006634:Histamine H1 Antagonists; D003189:p-Methoxy-N-methylphenethylamine; D010047:Ovalbumin; D002974:Clemastine