Characterization of antigenic property of Toxocara canis and Toxascaris leonina adults and larvae through immunodiagnostic electrophoresis (SDS-PAGE) and western blot technique.
Differential molecular studies were performed by sodium dodecyle sulphate polyacrylamide gel electrophoresis--SDS-PAGE--between somatic antigen of Toxocara canis and Toxascaris leonina, adults and larvae, recovered from dogs. SDS-PAGE of both adults somatic antigen showed two closely similar bands (90.00, 91.95 KDa and 69.25-70.56 KDa). Each parasite had characteristic bands clustered in different molecular weights. While for their larval antigen, T. canis showed a very different antigenic profile when analysed in comparison to T. leonina antigen except at one band (66.85-66.89 KDa). The Western blot analysis showed four prominent bands represented immunoreaction between the separated somatic antigen of T. canis adults and experimentally immunized rabbit with the corresponding parasite (125.37, 117.73, 90.00 and 69.25 KDa). While separated antigen of T. leonina adults immune reacted with the corresponding hyperimmune rabbit sera at 119.04, 91.95 and 70.56 KDa. The Western blot showed cross reactive immune bands between T. canis and T. leonina adults somatic antigen at two bands (90.00, 91.95 KDa and 69.25-70.56 KDa). The polypeptide bands reacted at 125.37 KDa and 117.73 KDa can be used as specific finger print for T. canis adults while that at 119.04 KDa was specific for T. leonina adult worm.
el-Massry
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Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Egypt.
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D011817:Rabbits; D017227:Toxascariasis / Q000469:parasitology / Q000662:veterinary; D014114:Toxascaris / Q000254:growth & development / Q000276:immunology*; D017166:Toxocara canis / Q000254:growth & development / Q000276:immunology*; D014120:Toxocariasis / Q000469:parasitology
D000947:Antigens, Helminth