Effect of cyclosporine on human sperm motility in vitro.
Cyclosporine affects motility and viability of human sperm when incubated together in vitro. Sperm motility was almost reduced to nil following 10 min of incubation with cyclosporine at a concentration of 1 mg/mL. However, 200 micrograms/mL of the drug has no effect on motility and viability when tested for up to 60 min under standard laboratory conditions. Cyclosporine effect on sperm was both dose and time dependent. Sperm sensitivity and susceptibility to cyclosporine even to lower doses increased significantly following withdrawal of bovine serum albumin from the incubating medium. Compared to untreated controls, lactate dehydrogenase was estimated higher by more than 2 to 4 times in the sperm-free incubating media, suggesting an altered membrane porosity in the affected spermatozoa.
Misro
M M
MM
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, New Delhi, India.
Chaki
S P
SP
Srinivas
M
M
Chaube
S K
SK
D016572:Cyclosporine / Q000494:pharmacology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*
D007166:Immunosuppressive Agents; D016572:Cyclosporine