The convergent point of the endocytic and autophagic pathways in leydig cells.
Endocytic tracers and marker enzyme of lysosomes were used in the present study to analyze the processes of autophagocytosis and endocytosis, and the convergent point of these two pathways in Leydig cells. The endocytic and autophagic compartments can be easily identified in Leydig cells, which makes easier to define the stages of two pathways than was possible before. The evidences indicated that the late endosomes (dense MVBs) deliver their endocytosed gold tracers together with lysosomal enzymes to the early autophagosomes and they are the convergent point of the two pathways. During this convergent process, the early autophagosomes transform into late autophagosomes and the late endosomes transform into mature lysosomes.
Yi
J
J
Department of Biophysics and Cell Biology, Shanghai Second Medical University, China.
Tang
X M
XM
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006801:Humans; D007985:Leydig Cells / Q000502:physiology* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008928:Mitochondria / Q000648:ultrastructure; D009708:Nucleotidases / Q000378:metabolism; D010588:Phagosomes / Q000201:enzymology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
D009708:Nucleotidases; C025221:cytidine-5'-monophosphatase