Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.
We describe the characterization of siglec-8, a novel sialic acid-binding immunoglobulin-like lectin that is expressed specifically by eosinophils. A full-length cDNA encoding siglec-8 was isolated from a human eosinophil cDNA library. Siglec-8 is predicted to contain three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail of 47 amino acids. The siglec-8 gene mapped on chromosome 19q13.33-41, closely linked to genes encoding CD33 (siglec-3), siglec-5, siglec-6, and siglec-7. When siglec-8 was expressed on COS cells or as a recombinant protein fused to the Fc region of human IgG(1), it was able to mediate sialic acid-dependent binding to human erythrocytes and to soluble sialoglycoconjugates. Using specific monoclonal antibodies, siglec-8 could be detected only on eosinophils and hence appears to be the first example of an eosinophil-specific transmembrane receptor.
Floyd
H
H
The Wellcome Trust Biocentre at Dundee, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom.
Ni
J
J
Cornish
A L
AL
Zeng
Z
Z
Liu
D
D
Carter
K C
KC
Steel
J
J
Crocker
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D000944:Antigens, Differentiation, B-Lymphocyte / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000737:chemistry / Q000235:genetics; D019556:COS Cells; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D004804:Eosinophils / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D015723:Gene Library; D008040:Genetic Linkage; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics; D007074:Immunoglobulin G / Q000235:genetics; D037102:Lectins; D008214:Lymphocytes / Q000276:immunology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D063268:Sialic Acid Binding Ig-like Lectin 3; D012794:Sialic Acids / Q000097:blood; D014162:Transfection
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D037102:Lectins; D011993:Recombinant Fusion Proteins; C402206:SIGLEC7 protein, human; C403967:SIGLEC8 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3; D012794:Sialic Acids