title: Pegylation of magnetically oriented lipid bilayers.
abstract: We report NMR data for magnetically oriented phospholipid bilayers which have been doped with a lipid derivatized with a polyethylene glycol polymer headgroup to stabilize samples against aggregation. (13)C, (31)P, and (2)H NMR data indicate that the incorporation of PEG2000-PE (1% molar to DMPC) does not interfere with the orientation properties of bicelles prepared at 25% w/v with or without the presence of lanthanide. Bicelles prepared at 10% w/v are also shown to orient when PEG2000-PE is added. The addition of PEG2000-PE to cholesterol-containing, lanthanide-flipped bicelles is shown to inhibit sample phase separation and improve spectral quality. Furthermore, the addition of PEG2000-PE to high w/v bicelles (40% w/v) is demonstrated to lead to an increase in overall sample order.
authors.affiliation: Department of Chemistry, Swarthmore College, Swarthmore, Pennsylvania 19081, USA.
authors.forename: V
authors.initials: V
authors.lastname: King
authors.forename: M
authors.initials: M
authors.lastname: Parker
authors.forename: K P
authors.initials: KP
authors.lastname: Howard
mesh_terms: D002784:Cholesterol; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008674:Metals, Rare Earth; D010714:Phosphatidylethanolamines; D010742:Phospholipid Ethers; D011092:Polyethylene Glycols
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D008051:Lipid Bilayers; D008674:Metals, Rare Earth; D010714:Phosphatidylethanolamines; D010742:Phospholipid Ethers; C067972:dioleoyl-N-(monomethoxypolyethylene glycol succinyl)phosphatidylethanolamine; D011092:Polyethylene Glycols; D002784:Cholesterol