title: Expression of bcl-2 and Bax in EGFR-antisense transfected and untransfected glioblastoma cells.
abstract: Glioblastomas are the most frequent and most malignant astrocytic gliomas. The epidermal growth factor receptor (EGFR) gene is the most frequently amplified and overexpressed in glioblastomas. The expression of bcl-2 and Bax in EGFR-antisense transfected and un-transfected glioblastoma cell line, U87E and U87V was studied by immunohistochemistry and western blotting. Our results show that the expression of Bax was stronger and bcl-2 was weaker in EGFR-antisense transfected cell line than the untransfected control. Bcl-2 and Bax genes are probably involved in the reduction of malignancy of glioblastoma cell caused by the introduction of EGFR-antisense into these tumor cells.
authors.affiliation: Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T. Hong Kong.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wang
authors.forename: M X
authors.initials: MX
authors.lastname: Ding
authors.forename: J P
authors.initials: JP
authors.lastname: Yuan
authors.forename: M L
authors.initials: ML
authors.lastname: Jin
authors.forename: C F
authors.initials: CF
authors.lastname: Hao
authors.forename: S B
authors.initials: SB
authors.lastname: Chew-Cheng
authors.forename: H K
authors.initials: HK
authors.lastname: Ng
authors.forename: E C
authors.initials: EC
authors.lastname: Chew
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D066246:ErbB Receptors / Q000235:genetics*; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D009369:Neoplasms / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D014162:Transfection; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
chemical_list: C494826:BAX protein, human; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D066246:ErbB Receptors