title: Cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene (narB) from Rhodococcus sp. NCIMB12038.
abstract: Rhodococcus sp. NCIMB112038 can utilize naphthalene as its sole carbon and energy source. The gene encoding cis-naphthalene dihydrodiol dehydrogenase (narB) of this strain has been cloned and sequenced. Expression of NCIMB12038 cis-naphthalene dihydrodiol dehydrogenase was demonstrated in Escherichia coli cells. narB encodes a putative protein of 271 amino acids and shares 39% amino acid identity with the cis-naphthalene dihydrodiol dehydrogenase from Pseudomonas putida G7. Comparison of NarB with some putative cis-dihydrodiol dehydrogenases from Rhodococcus species revealed significant differences between these proteins. NarB together with two other proteins forms a new group of cis-dihydrodiol dehydrogenases.
authors.affiliation: The Questor Centre, David Keir Building, The Queen's University of Belfast, Stranmillis Road, Belfast, UK. l.kulakov@queens-belfast.ac.uk
authors.forename: L A
authors.initials: LA
authors.lastname: Kulakov
authors.forename: C C
authors.initials: CC
authors.lastname: Allen
authors.forename: D A
authors.initials: DA
authors.lastname: Lipscomb
authors.forename: M J
authors.initials: MJ
authors.lastname: Larkin
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001673:Biodegradation, Environmental; D002458:Cell Fractionation; D003001:Cloning, Molecular; D003470:Culture Media; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism*; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010802:Phylogeny; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; D009281:Naphthalenes; C031721:naphthalene; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C023125:dihydrodiol dehydrogenases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C116788:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase