title: Evidence for antigen presentation to sensitized T cells by thyroid peroxidase (TPO)-specific B cells in mice injected with fibroblasts co-expressing TPO and MHC class II.
abstract: Injection of AKR/N mice with fibroblasts co-expressing MHC class II and TPO in the absence of adjuvant induces IgG-class TPO antibodies that resemble spontaneously arising human thyroid autoantibodies. We have used this model to examine the effect of iodide on TPO antibody induction as well as to analyse the interaction between T and B cells. Despite its importance as a major environmental factor in thyroid autoimmunity, variable iodide intake had no detectable effects on TPO antibody levels, lymphocytic infiltration of the thyroid or thyroid hormone levels. In terms of T cell responsiveness, splenocytes from TPO fibroblast-injected mice, but not from control mice, proliferated in response to TPO. Intriguingly, B cell-depleted splenocytes (mainly T cells without reduction of macrophages) proliferated in response to TPO only when co-cultured with irradiated autologous splenocytes from TPO fibroblast-injected mice but not from control mice. These data suggest that TPO-specific B cells are involved in antigen presentation to sensitized T cells and are supported by the ability of spleen cells from TPO cell-injected (but not control) mice to secrete TPO antibodies spontaneously in culture. In conclusion, we provide the first evidence for the presence of thyroid autoantigen-specific B cells and their ability to present their autoantigen to sensitized T cells in mice induced to develop TPO antibodies resembling autoantibodies in humans.
authors.affiliation: Autoimmune Disease Unit, Cedars-Sinai Research Institute and UCLA School of Medicine, Los Angeles, CA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Rapoport
authors.forename: S M
authors.initials: SM
authors.lastname: McLachlan
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis; D015551:Autoimmunity; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000637:transplantation; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007453:Iodide Peroxidase / Q000276:immunology*; D007455:Iodine / Q000008:administration & dosage; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013961:Thyroid Gland / Q000201:enzymology / Q000276:immunology; D014182:Transplantation, Autologous
chemical_list: D001323:Autoantibodies; D000949:Histocompatibility Antigens Class II; D007455:Iodine; D007453:Iodide Peroxidase