title: Etiology of genital ulcer disease in Dakar, Senegal, and comparison of PCR and serologic assays for detection of Haemophilus ducreyi.
abstract: We used PCR assays to determine the etiology of genital ulcers in patients presenting to a sexually transmitted disease clinic in Dakar, Senegal, and evaluated the ability of two PCR tests (groEL and recD) and two serological tests (adsorption enzyme immunoassay [EIA] and lipooligosaccharide [LOS] EIA) to detect current Haemophilus ducreyi infection. We found that in this population, H. ducreyi, T. pallidum, and herpes simplex virus HSV DNA were detected in 56, 15, and 13% of 39 genital ulcer specimens, respectively, and H. ducreyi DNA was detected in 60% (3 of 5) of samples from ulcerated bubos. Among 40 consecutive patients with genital ulcer disease and with sufficient sample for both PCR assays, the recD and groEL H. ducreyi PCR assays were 83% concordant, with the recD PCR assay detecting six (15%) additional positive specimens and the groEL assay detecting one (3%) additional positive specimen. Compared to PCR, the adsorption EIA and LOS EIA tests had sensitivities of 71 and 59% and specificities of 57 and 90%, respectively, for the diagnosis of current H. ducreyi infection. While these differences in specificity could be due either to previous infection with H. ducreyi or to the detection of cross-reacting antibodies, only 6% of patients from a nearby family planning clinic gave a positive reaction in both the adsorption EIA and LOS EIA assays, indicating that cross-reacting antibodies are not prevalent among clinic attendees in this city. Our studies indicate that the adsorption EIA detects both current and past infection, while the LOS EIA assay is more specific for current infection with H. ducreyi in this population.
authors.affiliation: Department of Medicine, University of Washington, Seattle, Washington, USA. patotten@u.washington.edu
authors.forename: P A
authors.initials: PA
authors.lastname: Totten
authors.forename: J M
authors.initials: JM
authors.lastname: Kuypers
authors.forename: C Y
authors.initials: CY
authors.lastname: Chen
authors.forename: M J
authors.initials: MJ
authors.lastname: Alfa
authors.forename: L M
authors.initials: LM
authors.lastname: Parsons
authors.forename: S M
authors.initials: SM
authors.lastname: Dutro
authors.forename: S A
authors.initials: SA
authors.lastname: Morse
authors.forename: N B
authors.initials: NB
authors.lastname: Kiviat
mesh_terms: D000328:Adult; D001483:Base Sequence; D002602:Chancroid / Q000209:etiology*; D018834:Chaperonin 60 / Q000235:genetics; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics; D005260:Female; D052776:Female Urogenital Diseases / Q000209:etiology*; D006191:Haemophilus ducreyi / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D052801:Male Urogenital Diseases; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012675:Senegal; D012883:Skin Ulcer / Q000209:etiology*
chemical_list: D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V; C468322:exodeoxyribonuclease V, E coli