title: Helicobacter pylori type I strains among Austrian and Portuguese patients with gastritis, peptic ulcer or gastric cancer.
abstract: The frequency of occurrence of Helicobacter pylori type I strains in isolates from Austria and Portugal and different polymerase chain reaction-based approaches to detect the cag pathogenicity island were assessed. Of the 41 Austrian strains, eight of 14 (57.2%) isolated from patients with gastritis, 14 of 19 (73.7%) from patients with peptic ulcer and eight of eight (100%) from patients with gastric cancer were type I strains. Among the Portuguese strains, eight of 14 (57.2%) isolated from patients with gastritis, ten of 12 (83.3%) from patients with peptic ulcer and five of 13 (38.5%) from patients with gastric cancer were classified as type I. Thus, Helicobacter pylori type I strains occur frequently in both populations but show no significant correlation with peptic ulcer disease. The prevalence of the type Igenotype in Austrian cancer patients, however, was significantly higher (P=0.007). The cagE-specific polymerase chain reaction was found to be a reliable and efficient method for detection of the cag pathogenicity island.
authors.affiliation: Department of Clinical Microbiology, Hygiene Institute, University of Vienna, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Bach
authors.forename: A
authors.initials: A
authors.lastname: Makristathis
authors.forename: A
authors.initials: A
authors.lastname: Pinto
authors.forename: M
authors.initials: M
authors.lastname: Quina
authors.forename: M
authors.initials: M
authors.lastname: Rotter
authors.forename: A M
authors.initials: AM
authors.lastname: Hirschl
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial; D001317:Austria; D001426:Bacterial Proteins / Q000235:genetics; D005756:Gastritis / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D010437:Peptic Ulcer / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011174:Portugal; D013274:Stomach Neoplasms / Q000382:microbiology*; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori