title: Isolation and characterization of Escherichia coli O157 from retail beef and bovine feces in Thailand.
abstract: Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine feces samples collected in Southern Thailand by enrichment culture followed by immunomagnetic bead separation. Four of the five strains carried the stx(2) gene alone or in combination with the stx(1) gene. The strains were shown to be genetically distinct by an arbitrarily primed PCR method.
authors.affiliation: Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat-yai, Thailand.
authors.forename: V
authors.initials: V
authors.lastname: Vuddhakul
authors.forename: N
authors.initials: N
authors.lastname: Patararungrong
authors.forename: P
authors.initials: P
authors.lastname: Pungrasamee
authors.forename: S
authors.initials: S
authors.lastname: Jitsurong
authors.forename: T
authors.initials: T
authors.lastname: Morigaki
authors.forename: N
authors.initials: N
authors.lastname: Asai
authors.forename: M
authors.initials: M
authors.lastname: Nishibuchi
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001427:Bacterial Toxins / Q000235:genetics; D002417:Cattle; D003470:Culture Media; D016172:DNA Fingerprinting; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D005511:Food Handling; D006801:Humans; D018189:Immunomagnetic Separation; D008460:Meat / Q000382:microbiology*; D019081:O Antigens / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D022603:Shiga Toxins; D013785:Thailand
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D003470:Culture Media; D019081:O Antigens; D022603:Shiga Toxins