title: Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen.
abstract: Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.
authors.affiliation: Department of Clinical Chemistry, Helsinki University Central Hospital, Finland.
authors.forename: U H
authors.initials: UH
authors.lastname: Stenman
authors.forename: E
authors.initials: E
authors.lastname: Paus
authors.forename: W J
authors.initials: WJ
authors.lastname: Allard
authors.forename: I
authors.initials: I
authors.lastname: Andersson
authors.forename: C
authors.initials: C
authors.lastname: Andrès
authors.forename: T R
authors.initials: TR
authors.lastname: Barnett
authors.forename: C
authors.initials: C
authors.lastname: Becker
authors.forename: A
authors.initials: A
authors.lastname: Belenky
authors.forename: L
authors.initials: L
authors.lastname: Bellanger
authors.forename: C M
authors.initials: CM
authors.lastname: Pellegrino
authors.forename: O P
authors.initials: OP
authors.lastname: Børmer
authors.forename: G
authors.initials: G
authors.lastname: Davis
authors.forename: B
authors.initials: B
authors.lastname: Dowell
authors.forename: L S
authors.initials: LS
authors.lastname: Grauer
authors.forename: D C
authors.initials: DC
authors.lastname: Jette
authors.forename: B
authors.initials: B
authors.lastname: Karlsson
authors.forename: F T
authors.initials: FT
authors.lastname: Kreutz
authors.forename: T M
authors.initials: TM
authors.lastname: van der Kwast
authors.forename: L
authors.initials: L
authors.lastname: Lauren
authors.forename: M
authors.initials: M
authors.lastname: Leinimaa
authors.forename: J
authors.initials: J
authors.lastname: Leinonen
authors.forename: H
authors.initials: H
authors.lastname: Lilja
authors.forename: H J
authors.initials: HJ
authors.lastname: Linton
authors.forename: M
authors.initials: M
authors.lastname: Nap
authors.forename: J
authors.initials: J
authors.lastname: Hilgers
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D003429:Cross Reactions; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008958:Models, Molecular; D017430:Prostate-Specific Antigen / Q000276:immunology*; D017434:Protein Structure, Tertiary; D009626:Terminology as Topic
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D017430:Prostate-Specific Antigen