title: Substitution of amino acid residue in influenza A virus hemagglutinin affects recognition of sialyl-oligosaccharides containing N-glycolylneuraminic acid.
abstract: Sialic acids are essential components of cell surface receptors used by influenza viruses. To determine the molecular mechanisms of viral recognition of two major species of sialic acids, N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc), we tested the binding reactivity of nine human H3 influenza A viruses to sialylglycolipids containing type II sugar chain and different molecular species of terminal sialic acids. All human H3 viruses tested except A/Memphis/1/71 bound both Neu5Ac and Neu5Gc. Nucleotide sequence analysis suggests that amino acids at 143, 155, and 158 are linked to the viral recognition of Neu5Gc.
authors.affiliation: Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Sciences, Shizuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Masuda
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: G
authors.initials: G
authors.lastname: Horiike
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: D
authors.initials: D
authors.lastname: Miyamoto
authors.forename: K I
authors.initials: KI
authors.lastname: Jwa Hidari
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Kida
authors.forename: M
authors.initials: M
authors.lastname: Kiso
authors.forename: K
authors.initials: K
authors.lastname: Fukunaga
authors.forename: M
authors.initials: M
authors.lastname: Ohuchi
authors.forename: T
authors.initials: T
authors.lastname: Toyoda
authors.forename: A
authors.initials: A
authors.lastname: Ishihama
authors.forename: Y
authors.initials: Y
authors.lastname: Kawaoka
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D006001:Glycoconjugates / Q000378:metabolism; D006388:Hemagglutinins / Q000737:chemistry*; D006801:Humans; D009980:Influenza A virus / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012795:Sialoglycoproteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D006001:Glycoconjugates; D006388:Hemagglutinins; D009438:Neuraminic Acids; D012795:Sialoglycoproteins; D014764:Viral Proteins; C032592:N-glycolylneuraminic acid