Simultaneous determination of fumonisin B(1) and hydrolyzed fumonisin B(1) in corn products by liquid chromatography/electrospray ionization mass spectrometry.
A method for the simultaneous determination of fumonisin B(1) (FB(1)) and its major hydrolysis product (HFB(1)), which is known to be formed during alkaline treatment of fumonisin-containing corn meal, was devised to analyze the levels of these mycotoxins in corn products available on the German market. Liquid chromatography/electrospray mass spectrometry in combination with selected ion monitoring (SIM) was used for unambiguous detection of FB(1) and HFB(1) after extraction of samples with acetonitrile/methanol/water (25:25:50) and solid-phase C18 cleanup. Quantitation was carried out using labeled fumonisin FB(1)-D(6) as an internal standard. The detection limits achieved with this method were 8 ng/g for HFB(1) (signal-noise ratio = 5:1) and 5 ng/g for FB(1) (s/n = 5:1) using the protonated molecule signals m/z 406 and 722 in the SIM mode. A screening of several corn-containing foodstuffs, among them extrusion products and alkali-processed corn food such as tortilla chips, showed HFB(1) and FB(1) contamination with levels of 8-80 and 5-450 ng/g, respectively.
Hartl
M
M
Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.
Humpf
H U
HU
D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D002264:Carboxylic Acids / Q000032:analysis*; D005516:Food Microbiology; D037341:Fumonisins; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D013605:T-2 Toxin / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology*
D002264:Carboxylic Acids; D037341:Fumonisins; C056933:fumonisin B1; D013605:T-2 Toxin