title: Simultaneous measurement of cefuroxime in rat blood and brain by microdialysis and microbore liquid chromatography. Application to pharmacokinetics.
abstract: To characterize the pharmacokinetics of cefuroxime in rat blood and brain, microdialysis probes were inserted into the jugular vein and brain striatum, respectively. Cefuroxime (20 mg/kg, i.v.) was administered via the femoral vein. Blood microdialysates were automatic injected onto microbore liquid chromatography via an on-line injectors. The mobile phase consisted of methanol-100 mM monosodium phosphoric acid (25:75, v/v, pH 5.0) with a flow-rate of 0.05 ml/min. Ultraviolet detector was set at a wavelength of 280 nm for cefuroxime. The present assay enhanced the detection sensitivity and enabled the determination of cefuroxime down to 5 ng/ml. The pharmacokinetic data demonstrated that the area under the concentration curve (AUC) ratio of unbound cefuroxime in rat brain and blood was about 4.2% after cefuroxime (20 mg/kg, i.v.) administration. These results provided further evidence that cefuroxime could penetrate the blood-brain barrier.
authors.affiliation: Department of Pharmacology, National Research Institute of Chinese Medicine, Taipei, Taiwan. thtsai@cma23.nricm.edu.tw
authors.forename: T H
authors.initials: TH
authors.lastname: Tsai
authors.forename: F C
authors.initials: FC
authors.lastname: Cheng
authors.forename: K C
authors.initials: KC
authors.lastname: Chen
authors.forename: Y F
authors.initials: YF
authors.lastname: Chen
authors.forename: C F
authors.initials: CF
authors.lastname: Chen
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002444:Cefuroxime / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D002511:Cephalosporins / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008297:Male; D000432:Methanol; D017551:Microdialysis; D010756:Phosphoric Acids; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
chemical_list: D002511:Cephalosporins; D010756:Phosphoric Acids; C030242:phosphoric acid; D002444:Cefuroxime; D000432:Methanol