Metal regulation of metallothionein participation in redox reactions.
Like glutathione or dithiothreitol, metallothionein effects the formation of pancreatic ribonuclease A from its S-sulfonated derivative catalyzed by protein disulfide isomerase. EDTA increases the yield of ribonuclease A activity recovery with metallothionein but does not affect the reaction with glutathione or dithiothreitol. EDTA also increases the reactivity of thiol groups in metallothionein with 5,5'-dithiobis-(2-nitrobenzoic acid) by chelation of zinc ions. It is suggested that the thiol groups in metallothionein form a part of the pool of cellular thiols in the regulation of cellular redox reactions and their availability is modulated by zinc chelation.
Zhang
S
S
National Laboratory of Biomacromolecules, Institute of Biophysics, Beijing, China.
Li
J
J
Wang
C C
CC
Tsou
C L
CL
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology; D004229:Dithiothreitol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D011817:Rabbits; D012259:Ribonuclease, Pancreatic / Q000096:biosynthesis*; D013997:Time Factors; D015032:Zinc / Q000378:metabolism*
D002614:Chelating Agents; D008668:Metallothionein; D004492:Edetic Acid; D012259:Ribonuclease, Pancreatic; D019704:Protein Disulfide-Isomerases; D005978:Glutathione; D015032:Zinc; D004229:Dithiothreitol