Induction of gp130 and LIF by differentiation inducers in human myeloid leukemia K562 cells.
It has been previously shown that phorbol 12-myristate 13-acetate (PMA), a potent differentiation inducer, induced the expression of both interleukin-6 (IL-6) and IL-6 receptor alpha component (IL-6Ralpha) in K562 leukemia cells. In the present study, we examined the ability of several differentiation inducers to regulate the expression of the signal-transducing receptor component for IL-6, gp130, and cytokine leukemia inhibitory factor (LIF) in K562 cells. We found that the expression of gp130 was dramatically induced at both the mRNA and protein levels by the two megakaryocytic inducers sodium butyrate (NaBut) and PMA. In contrast, the mRNA expression of LIF was induced by the two erythroid inducers 1-beta-D-arabinofuranosyl cytosine (Ara-C) and hemin. Furthermore, activation of the PMA-induced gp130 receptor by exogenous IL-6 potentiated the differentiating effects of PMA. Our findings suggest that IL-6/gp130 signaling may be involved in the regulation of the megakaryocytic differentiation of K562 cells.
Xie
P
P
Department of Biology, Biotechnology Research Institute, The Hong Kong University of Science and Technology, People's Republic of China.
Chan
F S
FS
Ip
N Y
NY
Leung
M F
MF
D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis / Q000235:genetics; D015415:Biomarkers; D002087:Butyrates / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003561:Cytarabine / Q000494:pharmacology; D050822:Cytokine Receptor gp130; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D004920:Erythropoiesis / Q000187:drug effects*; D006021:Glycophorins / Q000096:biosynthesis / Q000235:genetics; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D020014:K562 Cells / Q000187:drug effects*; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D009283:Naphthol AS D Esterase / Q000096:biosynthesis / Q000235:genetics; D011990:Receptors, Transferrin; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D015415:Biomarkers; D002087:Butyrates; C068126:CD71 antigen; D006021:Glycophorins; D006131:Growth Inhibitors; C494199:IL6ST protein, human; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D008562:Membrane Glycoproteins; D011990:Receptors, Transferrin; D003561:Cytarabine; D050822:Cytokine Receptor gp130; D014212:Tretinoin; D006427:Hemin; D009283:Naphthol AS D Esterase; D013755:Tetradecanoylphorbol Acetate