Improving effects of the extracts from Eugenia uniflora on hyperglycemia and hypertriglyceridemia in mice.
EtOH (70%) extracts from the leaves of Eugenia uniflora were separated into six fractions with different polarity and molecular size, i.e. NP-1-NP-6. In an oral glucose tolerance test, NP-1 and 4 inhibited the increase in plasma glucose level. However, in an intraperitoneal glucose tolerance test, such an inhibitory effect was not seen. Thus, the effects of NP-1 and 4 were apparently due to the inhibition of glucose absorption from the intestine. In a sucrose tolerance test, all fractions inhibited the increase in plasma glucose level. In an oral corn oil tolerance test, NP-3 and 4 showed an inhibitory effect on the increase in plasma triglycerides level. On the other hand, NP-3, 4, 5 and 6 inhibited maltase and sucrase activities and all fractions except for NP-1 showed an inhibitory effect on lipase activity dose-dependently. The inhibition of the increase in plasma glucose level by NP-3, 4, 5 and 6 in the oral sucrose tolerance test and the inhibition of the increase in plasma triglycerides by NP-3 and 4 in the oral corn oil tolerance test were apparently due to the inhibition of the decomposition of carbohydrates and fats in the intestine, respectively.
Arai
I
I
Central Research Laboratories, Tsumura & Company, Ibaraki, Japan. arai_ichirou@mail.tsumura.co.jp
Amagaya
S
S
Komatsu
Y
Y
Okada
M
M
Hayashi
T
T
Kasai
M
M
Arisawa
M
M
Momose
Y
Y
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D005959:Glucosidases / Q000378:metabolism; D006943:Hyperglycemia / Q000188:drug therapy*; D015228:Hypertriglyceridemia / Q000188:drug therapy*; D007408:Intestinal Absorption / Q000187:drug effects; D008049:Lipase / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000008:administration & dosage* / Q000627:therapeutic use; D018515:Plant Leaves / Q000737:chemistry*; D012995:Solubility; D013393:Sucrase / Q000378:metabolism; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D000520:alpha-Glucosidases / Q000378:metabolism
D001786:Blood Glucose; D010936:Plant Extracts; D014280:Triglycerides; D000431:Ethanol; D008049:Lipase; D005959:Glucosidases; D000520:alpha-Glucosidases; D013393:Sucrase; D005947:Glucose