Summary report of the TD-3 workshop: characterization of 83 antibodies against prostate-specific antigen.
Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.
Stenman
U H
UH
Department of Clinical Chemistry, Helsinki University Central Hospital, Finland.
Paus
E
E
Allard
W J
WJ
Andersson
I
I
Andrès
C
C
Barnett
T R
TR
Becker
C
C
Belenky
A
A
Bellanger
L
L
Pellegrino
C M
CM
Børmer
O P
OP
Davis
G
G
Dowell
B
B
Grauer
L S
LS
Jette
D C
DC
Karlsson
B
B
Kreutz
F T
FT
van der Kwast
T M
TM
Lauren
L
L
Leinimaa
M
M
Leinonen
J
J
Lilja
H
H
Linton
H J
HJ
Nap
M
M
Hilgers
J
J
D000911:Antibodies, Monoclonal / Q000737:chemistry; D003429:Cross Reactions; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008958:Models, Molecular; D017430:Prostate-Specific Antigen / Q000276:immunology*; D017434:Protein Structure, Tertiary; D009626:Terminology as Topic
D000911:Antibodies, Monoclonal; D000939:Epitopes; D017430:Prostate-Specific Antigen