Increased sensitivity to oxidative challenges associated with topA deletion in Escherichia coli.
Deletion of topA in Escherichia coli was found to result in a higher level of killing after treatment with either hydrogen peroxide or N-ethylmaleimide. This effect on oxidative challenge response represents a new role for E. coli DNA topoisomerase I in addition to prevention of excessive negative supercoiling of DNA.
Tse-Dinh
Y C
YC
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA. yuk-ching_tse-dinh@nymc.edu
D004264:DNA Topoisomerases, Type I / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005033:Ethylmaleimide / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010084:Oxidation-Reduction; D017384:Sequence Deletion
D006861:Hydrogen Peroxide; D004264:DNA Topoisomerases, Type I; D005033:Ethylmaleimide