title: Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments.
abstract: In experiments with proteins of molecular weights around 100 kDa the implementation of [15N,1H]-TROSY-elements in [15N]-constant-time triple resonance experiments yields sensitivity enhancements of one to two orders of magnitude. An additional gain of 10 to 20% may be obtained with the use of 'sensitivity enhancement elements'. This paper describes a novel sensitivity enhancement scheme which is based on concatenation of the 13C alpha-->15N magnetization transfer with the ST2-PT element, and which enables proper TROSY selection of the 15N multiplet components.
authors.affiliation: Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, Zürich, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Salzmann
authors.forename: G
authors.initials: G
authors.lastname: Wider
authors.forename: K
authors.initials: K
authors.lastname: Pervushin
authors.forename: K
authors.initials: K
authors.lastname: Wüthrich
mesh_terms: D000446:Aldehyde-Lyases / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D008280:Magnetics; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D000446:Aldehyde-Lyases; C020044:dihydroneopterin aldolase