Uptake of 2-oxoglutarate in Synechococcus strains transformed with the Escherichia coli kgtP gene.
A number of cyanobacteria from different taxonomic groups exhibited very low levels of uptake of 2-[U-(14)C]oxoglutarate. Synechococcus sp. strain PCC 7942 was transformed with DNA constructs carrying the Escherichia coli kgtP gene encoding a 2-oxoglutarate permease and a kanamycin resistance gene cassette. The Synechococcus sp. strains bearing the kgtP gene incorporated 2-oxoglutarate into the cells through an active transport process. About 75% of the radioactivity from the 2-[U-(14)C]oxoglutarate taken up that was recovered in soluble metabolites was found as glutamate and glutamine. 2-Oxoglutarate was, however, detrimental to the growth of a Synechococcus sp. strain bearing the kgtP gene.
Vázquez-Bermúdez
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MF
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, E-41092 Seville, Spain.
Herrero
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D001692:Biological Transport; D002250:Carbon Radioisotopes; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D029382:Dicarboxylic Acid Transporters; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D007656:Ketoglutaric Acids / Q000378:metabolism / Q000493:pharmacokinetics*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D014169:Transformation, Bacterial / Q000235:genetics
D002250:Carbon Radioisotopes; D002352:Carrier Proteins; D029382:Dicarboxylic Acid Transporters; D029968:Escherichia coli Proteins; D007656:Ketoglutaric Acids; D026901:Membrane Transport Proteins; C068628:KgtP protein, E coli