Efficient method for (lyso)phospholipid class separation by high-performance liquid chromatography using an evaporative light-scattering detector.
A simple high-performance liquid chromatography method with evaporative light-scattering detection has been devised in order to separate and quantify the major phospholipid and lysophospholipid classes. HPLC analyses were performed with a diol-silica column and ternary gradient elution. Standard curves were drawn up for each of the (lyso)phospholipids involved.
Sas
B
B
R&D Department, Kemin Europa N.V., Herentals, Belgium.
Peys
E
E
Helsen
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D008246:Lysophospholipids / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification*; D012542:Scattering, Radiation
D008246:Lysophospholipids; D010743:Phospholipids