title: [Quantitative gas chromatography-mass spectrometry determination of pentaerythrityl tetranitrate metabolites pentaerythrityl trinitrate, pentaerythrityl dinitrate and pentaerythrityl in human plasma].
abstract: Assay methods based on gas chromatography/mass spectroscopy (GC-MS) may be used to measure PE1N (pentaerithrityl mononitrate, CAS 1607-00-7), PE2N (pentaerithrityl dinitrate, CAS 1607-01-8) and intermediate pentaerithrityltrinitrate (PE3N, CAS 1607-17-6) in human plasma. Based on this method a simplified method to quantify the metabolites of PETN (pentaerithrityl tetranitrate, CAS 78-11-5, Pentalong) is described. In the present study a consistent method to extract the metabolites of human plasma and following derivatisation is described. Since PE1N can be quantified up to 150 ng/ml, PE2N and PE3N up to 30 ng/ml in human plasma, a dilution of the plasma samples can be avoided. The mean recovery rate is not so high as in other described methods, and inaccuracy is about 10%. Therefore a calibration range between 0.2-30 ng/ml of PE2N and 1-150 ng/ml of PE1N has to be considered. The described method offers an alternative and applicable option to quantify the PETN-metabolites and elucidate their part as NO-donors.
authors.affiliation: ISIS Pharma GmbH, Zwickau/Monheim.
authors.forename: A
authors.initials: A
authors.lastname: Schütz
authors.forename: J
authors.initials: J
authors.lastname: Kötting
authors.forename: F
authors.initials: F
authors.lastname: Epple
authors.forename: R
authors.initials: R
authors.lastname: Ziegler
authors.forename: H
authors.initials: H
authors.lastname: Maier-Lenz
authors.forename: D
authors.initials: D
authors.lastname: Stalleicken
mesh_terms: D001711:Biotransformation; D002138:Calibration; D004896:Erythritol / Q000031:analogs & derivatives* / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010417:Pentaerythritol Tetranitrate / Q000031:analogs & derivatives / Q000097:blood* / Q000493:pharmacokinetics; D014665:Vasodilator Agents / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D014665:Vasodilator Agents; D010417:Pentaerythritol Tetranitrate; C403269:pentaerythritol dinitrate; C100210:pentrinitrol; C035013:pentaerythritol mononitrate; D004896:Erythritol