HeT-A telomere-specific retrotransposons in the centric heterochromatin of Drosophila melanogaster chromosome 3.
We have isolated two yeast artificial chromosome (YAC) clones from Drosophila melanogaster that contain a small amount of dodeca satellite (a satellite DNA located in the centromeric region of chromosome 3) and sequences homologous to the telomeric retro-transposon HeT-A. Using these YACs as probes for fluorescence in situ hybridization to mitotic chromosomes, we have localized these HeT-A elements to the centric heterochromatin of chromosome 3, at region h55. The possible origin of these telomeric elements in a centromeric position is discussed.
Losada
A
A
Centro de Biología Molecular Severo Ochoa (CSIC_UAM), Madrid, Spain.
Agudo
M
M
Abad
J P
JP
Villasante
A
A
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes; D018244:Chromosomes, Artificial, Yeast; D017931:DNA Primers; D004331:Drosophila melanogaster / Q000235:genetics*; D006570:Heterochromatin / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D018626:Retroelements; D016615:Telomere
D017931:DNA Primers; D006570:Heterochromatin; D018626:Retroelements