Characterization of cDNA and expression of mRNA encoding an Achatina cardioexcitatory RFamide peptide.
Achatina cardioexcitatory peptide-1 (ACEP-1) is an RFamide family peptide isolated from the atria of the African giant snail, Achatina fulica. In this report, we describe an identification of the ACEP-1 cDNA sequence and localizations of the ACEP-1 mRNA. Southern blot analysis revealed that the ACEP-1 mRNA was present in the atrium as well as in the central nervous system. Furthermore, in situ hybridization revealed the localizations of the ACEP-1 mRNA in small neurons of the cerebral and pedal ganglia and a few large neurons of the right parietal and visceral ganglia.
Satake
H
H
Suntory Institute for Bioorganic Research, Mishima-gun, Osaka, Japan.
Takuwa
K
K
Minakata
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics*; D006325:Heart Atria / Q000737:chemistry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012908:Snails / Q000235:genetics*
D018076:DNA, Complementary; D009479:Neuropeptides; D011498:Protein Precursors; D012333:RNA, Messenger; C058047:arginylphenylalaninamide