Growth and survival of various strains of enterohemorrhagic Escherichia coli in hydrochloric and acetic acid.
Nineteen strains of enterohemorrhagic Escherichia coli isolated from humans and foods were examined for their ability to grow and survive at low pH in organic (acetic) and mineral (HCl) acids. Strains were subcultured in tryptic soy broth adjusted to various pH values (3.75 to 4.75 for HCl and 4.75 to 5.75 for acetic acid) and incubated for 72 h at 37 degrees C to determine the minimum growth pH value. Minimum pH values for growth of 4.25 and 5.5 were found for HCl and acetic acid, respectively. Strains were also exposed to pH 2.0 (HCl) and pH 4.0 (acetic acid) for up to 24 h at 37 degrees C to assess their ability to survive. HCl was a more effective inhibitor after 6 h of exposure, whereas acetic acid was more effective after 24 h. Outbreak strains survived acid treatment significantly (P < or = 0.05) better than strains isolated from fermented or high-pH foods or animal or human isolates. Significant (P < or = 0.05) differences among serotypes and between O157:H7 and other serotypes were apparent after 3 or 6 h of exposure to acids.
McKellar
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Southern Crop Protection and Food Research Centre, Food Research Program, Research Branch, Agriculture and Agri-Food Canada, Guelph, Ontario, Canada. mckellarr@em.agr.ca
Knight
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D019342:Acetic Acid / Q000494:pharmacology*; D000818:Animals; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000145:classification / Q000254:growth & development* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D005516:Food Microbiology; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D012703:Serotyping
D019342:Acetic Acid; D006851:Hydrochloric Acid