title: Relish, a central factor in the control of humoral but not cellular immunity in Drosophila.
abstract: The NF-kappa B-like Relish gene is complex, with four transcripts that are all located within an intron of the Nmdmc gene. Using deletion mutants, we show that Relish is specifically required for the induction of the humoral immune response, including both antibacterial and antifungal peptides. As a result, the Relish mutants are very sensitive to infection. A single cell of E. cloacae is sufficient to kill a mutant fly, and the mutants show increased susceptibility to fungal infection. In contrast, the blood cell population, the hematopoietic organs, and the phagocytic, encapsulation, and melanization responses are normal. Our results illustrate the importance of the humoral response in Drosophila immunity and demonstrate that Relish plays a key role in this response.
authors.affiliation: Umeå Center for Molecular Pathogenesis, Umeå University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Hedengren
authors.forename: B
authors.initials: B
authors.lastname: Asling
authors.forename: M S
authors.initials: MS
authors.lastname: Dushay
authors.forename: I
authors.initials: I
authors.lastname: Ando
authors.forename: S
authors.initials: S
authors.lastname: Ekengren
authors.forename: M
authors.initials: M
authors.lastname: Wihlborg
authors.forename: D
authors.initials: D
authors.lastname: Hultmark
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000378:metabolism; D000917:Antibody Formation / Q000235:genetics / Q000276:immunology*; D000935:Antifungal Agents / Q000378:metabolism; D001419:Bacteria / Q000276:immunology; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D005091:Exons / Q000235:genetics; D005658:Fungi / Q000276:immunology; D017344:Genes, Insect / Q000235:genetics / Q000502:physiology; D006434:Hemocytes / Q000276:immunology; D007111:Immunity, Cellular / Q000235:genetics / Q000276:immunology*; D007438:Introns / Q000235:genetics; D007814:Larva / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D018448:Models, Immunological; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010587:Phagocytosis / Q000276:immunology; D017384:Sequence Deletion / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000935:Antifungal Agents; D029721:Drosophila Proteins; D010455:Peptides; C101626:Rel protein, Drosophila; D014157:Transcription Factors