Paraflagellar rod proteins administered with alum and IL-12 or recombinant adenovirus expressing IL-12 generates antigen-specific responses and protective immunity in mice against Trypanosoma cruzi.
Successful vaccination of mice against an otherwise lethal challenge with the Peru strain of Trypanosoma cruzi necessitates the induction of a strong cell mediated immune response. Previously, immunization of mice with the paraflagellar rod proteins from Trypanosoma cruzi90% reduction in parasitemia in immunized mice challenged with the bloodstream stage of Trypanosoma cruzi.
Wrightsman
R A
RA
Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92696-3900, USA.
Manning
J E
JE
D000256:Adenoviridae / Q000235:genetics; D000534:Alum Compounds / Q000008:administration & dosage; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D014355:Chagas Disease / Q000517:prevention & control*; D007109:Immunity; D018664:Interleukin-12 / Q000008:administration & dosage / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000469:parasitology; D051379:Mice; D008810:Mice, Inbred C57BL; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology*
D000534:Alum Compounds; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; C045519:paraflagellar rod proteins, Trypanosoma brucei; D018664:Interleukin-12; C041524:aluminum sulfate