title: Reduction in susceptibility to Brugia malayi of F2 progeny of Aedes togoi treated with ethyl methanesulfonate.
abstract: The susceptibility to Brugia malayi infection was tested in F2 female progeny derived from male and female Aedes togoi treated with ethyl methanesulfonate (EMS). Three-day-old males and females were treated with 0.025, 0.050, and 0.075, 0.10, 0.15, or 0.20% EMS by allowing them to feed for 5 days on sugar cubes containing EMS and then mated at random. Percentage of susceptibility and mean number of infective larvae (L3) in F2 females were analyzed over a 2-wk period. Reductions in susceptibility were significant in the F2 populations arising from the 3 highest EMS concentrations. F2 infections were reduced by 80%, indicating that EMS-induced mutations affect loci associated with filarial nematode susceptibility.
authors.affiliation: Division of Life Sciences, University of Texas at San Antonio, 78249, USA.
authors.forename: P H
authors.initials: PH
authors.lastname: Rodriguez
authors.forename: R
authors.initials: R
authors.lastname: Castillon
authors.forename: P
authors.initials: P
authors.lastname: Wilson
authors.forename: D
authors.initials: D
authors.lastname: Benoit
authors.forename: D
authors.initials: D
authors.lastname: Nasr-Schirf
mesh_terms: D000330:Aedes / Q000235:genetics / Q000469:parasitology*; D000818:Animals; D017178:Brugia malayi / Q000472:pathogenicity*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D005260:Female; D007303:Insect Vectors; D007814:Larva / Q000469:parasitology; D008297:Male; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology*
chemical_list: D009153:Mutagens; D005020:Ethyl Methanesulfonate