Species identification and strain typing of Malassezia species stock strains and clinical isolates based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions.
This study demonstrated the application of internal transcribed spacer 1 (ITS1) ribosomal DNA sequences to the species identification and strain typing of 28 standard strains and 46 clinical isolates of the genus Malassezia. The size of ITS1 regions ranged from 162 to 266 bp. Members of the genus Malassezia (M. pachydermatis, M. furfur, M. sympodialis, M. globosa, M. obtusa, M. restricta and M. slooffiae) were classified into seven ITS1-homologous groups and 22 ITS1-identical, individual groups. The 46 clinical isolates of lipophilic Malassezia spp. were identified as belonging to just three ITS1-homologous groups, i.e., M. furfur (19 strains: 11 from pityriasis versicolor, 4 from seborrhoeic dermatitis and 4 from atopic dermatitis). M. sympodialis (22 strains: 7 from pityriasis versicolor, 3 from seborrhoeic dermatitis, 1 from atopic dermatitis and 11 from healthy controls) and M. slooffiae (five strains: three from chronic otitis media and two from healthy controls).
Makimura
Koichi
K
Teikyo University, Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.
Tamura
Yoshiko
Y
Teikyo University, Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.
Kudo
Michinari
M
Teikyo University, Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.
Uchida
Katsuhisa
K
Teikyo University, Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.
Saito
Hiuga
H
Teikyo University, Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.
Yamaguchi
Hideyo
H
Teikyo University, Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan.
D001483:Base Sequence; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry*; D003881:Dermatomycoses / Q000382:microbiology*; D006801:Humans; D008290:Malassezia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D004275:DNA, Ribosomal