title: Disruption of aldA influences the developmental process in Myxococcus xanthus.
abstract: Previously, we identified a gene (aldA) from Myxococcus xanthus, which we suggested encoded the enzyme alanine dehydrogenase on the basis of similarity to known Ald protein sequences (M. J. Ward, H. Lew, A. Treuner-Lange, and D. R. Zusman, J. Bacteriol. 180:5668-5675, 1998). In this study, we have confirmed that aldA does encode a functional alanine dehydrogenase, since it catalyzes the reversible conversion of alanine to pyruvate and ammonia. Whereas an aldA gene disruption mutation did not significantly influence the rate of growth or spreading on a rich medium, AldA was required for growth on a minimal medium containing L-alanine as the major source of carbon. Under developmental conditions, the aldA mutation caused delayed aggregation in both wild-type (DZ2) and FB (DZF1) strains. Poorly formed aggregates and reduced levels of spores were apparent in the DZ2 aldA mutant, even after prolonged development.
authors.affiliation: Department of Molecular Biology, University of California at Berkeley, Berkeley, California 94720, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ward
authors.forename: H
authors.initials: H
authors.lastname: Lew
authors.forename: D R
authors.initials: DR
authors.lastname: Zusman
mesh_terms: D050788:Alanine Dehydrogenase; D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D002384:Catalysis; D002874:Chromosome Mapping; D009154:Mutation; D016941:Myxococcus xanthus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*
chemical_list: D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase