title: Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA.
abstract: In this study, the gene encoding Babesia equi merozoite antigen-2 was inserted into a baculovirus transfer vector, and a recombinant virus expressing B. equi merozoite antigen-2 was isolated. Two B. equi merozoite antigen-2-related recombinant baculovirus-expressed peptides of 25 and 30 kDa were detected with a murine anti serum against B. equi merozoite antigen-2; these corresponded to the native B. equi merozoite antigen-2 and were secreted into the supernatants of insect cell cultures. Recombinant B. equi merozoite antigen-2 was not effected by tunicamycin treatment, indicating that B. equi merozoite antigen-2 was not glycosylated. The potential of recombinant B. equi merozoite antigen-2 for use in the ELISA was evaluated by measuring the antibody response to B. equi merozoite antigen-2 in horses experimentally infected with B. equi.
authors.affiliation: The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: X
authors.initials: X
authors.lastname: Xuan
authors.forename: H
authors.initials: H
authors.lastname: Ikadai
authors.forename: I
authors.initials: I
authors.lastname: Igarashi
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: K
authors.initials: K
authors.lastname: Fujisaki
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001403:Babesia / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001404:Babesiosis / Q000175:diagnosis; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006734:Horse Diseases / Q000175:diagnosis; D006736:Horses; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D017422:Sequence Analysis, DNA; D018411:Spodoptera
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C111468:EMA-2 protein, Babesia equi; D015800:Protozoan Proteins; D011994:Recombinant Proteins