Nonaqueous capillary electrophoresis: application possibilities and suitability of various solvents for the separation of basic analytes.
Eleven organic solvents were tested for their suitability in the use of capillary electrophoresis (CE). In all cases, 25 mM ammonium acetate and 1 M acetic acid were used as electrolytes. Three basic therapeutical agents, propranolol, carteolol and imipramine, were used as analytes. Four solvents (2-propanol, ethylene glycol, propylene glycol, dichloromethane) were not suitable for use with CE under our conditions. Depending on the other solvents used, the analytes showed very different behavior. We observed that the epsilon/eta quotient alone is not sufficient for a characterization of the solvents. Further investigations with all the solvents as 1:1 mixtures with methanol were carried out. As a result some principal changes occurred compared to the pure solvents. Working with mixtures of different solvents proved to be advantageous because of the possibility to influence properties like high viscosity or low ionizing abilities by the addition of a suitable second solvent.
Karbaum
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Institute of Pharmacy, Pharmaceutical/Medicinal Chemistry, Ernst-Moritz-Arndt-University Greifswald, Germany.
Jira
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T
D002354:Carteolol / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007099:Imipramine / Q000302:isolation & purification*; D011433:Propranolol / Q000302:isolation & purification*; D012997:Solvents
D012997:Solvents; D002354:Carteolol; D011433:Propranolol; D007099:Imipramine