title: The efficacy of laboratory diagnosis of Helicobacter pylori infections in gastric biopsy specimens is related to bacterial density and vacA, cagA, and iceA genotypes.
abstract: A total of 500 consecutive patients undergoing upper endoscopy were biopsied and tested for H. pylori infection by the Campylobacter-like organism (CLO) test, culture, histology, and PCR. Serum samples were tested by two different serological assays. Patients were considered H. pylori positive if at least two of the four biopsy specimen-based methods yielded positive results. PCR had the highest diagnostic sensitivity (99.4%), followed by histology (92.2%), culture (89.5%), and the CLO test (89.0%). The specificities of all methods were higher than 98%. Of the organisms from the 181 PCR-positive patients, the vacA (s and m regions), cagA, and iceA genotypes were determined by reverse hybridization (line probe assay) or an allele-specific PCR. Organisms that were detected by PCR but that remained undetected by the CLO test were significantly more often vacA s1 (P = 0.006), m1 (P = 0.028), and cagA positive (P = 0.029) than vacA s2, m2, and cagA negative, respectively. Organisms that were detected by PCR but that remained undetected by culture or histology more often contained iceA1 (P = 0. 034 and P = 0.029, respectively) than iceA2. Higher H. pylori density was associated with vacA s2 (P = 0.024), vacA m2 (P = 0.050), and cagA-negative (P = 0.035) genotypes. Also, the diagnostic results of the CLO test (P = 0.001) and culture (P = 0.031) but not those of the PCR (P = 0.130) were significantly associated with the H. pylori density. The rate of detection by the four biopsy specimen-based tests was lower for patients who used proton pump inhibitors, but this was independent of the H. pylori genotypes. These observations may be explained by different bacterial densities, as established by the distinct genotypes of H. pylori, and confirm that the biologies of strains with such genotypes are considerably different.
authors.affiliation: Delft Diagnostic Laboratory, Diagnostic Center SSDZ, Delft, The Netherlands. L.J.van.Doorn@ddl.nl
authors.forename: L J
authors.initials: LJ
authors.lastname: van Doorn
authors.forename: Y
authors.initials: Y
authors.lastname: Henskens
authors.forename: N
authors.initials: N
authors.lastname: Nouhan
authors.forename: A
authors.initials: A
authors.lastname: Verschuuren
authors.forename: R
authors.initials: R
authors.lastname: Vreede
authors.forename: P
authors.initials: P
authors.lastname: Herbink
authors.forename: G
authors.initials: G
authors.lastname: Ponjee
authors.forename: K
authors.initials: K
authors.lastname: van Krimpen
authors.forename: R
authors.initials: R
authors.lastname: Blankenburg
authors.forename: J
authors.initials: J
authors.lastname: Scherpenisse
authors.forename: W
authors.initials: W
authors.lastname: Quint
mesh_terms: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001431:Bacteriological Techniques; D001706:Biopsy; D002167:Campylobacter; D004724:Endoscopy; D005188:False Negative Reactions; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D016133:Polymerase Chain Reaction; D011706:Pyloric Antrum / Q000382:microbiology / Q000473:pathology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C063113:ice nucleation protein