title: The N-terminal region of the Escherichia coli WecA (Rfe) protein, containing three predicted transmembrane helices, is required for function but not for membrane insertion.
abstract: The correct site for translation initiation for Escherichia coli WecA (Rfe), presumably involved in catalyzing the transfer of N-acetylglucosamine 1-phosphate to undecaprenylphosphate, was determined by using its FLAG-tagged derivatives. The N-terminal region containing three predicted transmembrane helices was found to be necessary for function but not for membrane localization of this protein.
authors.affiliation: Department of Microbiology, University of Western Ontario, London, Ontario N6A 5C1, Canada.
authors.forename: A O
authors.initials: AO
authors.lastname: Amer
authors.forename: M A
authors.initials: MA
authors.lastname: Valvano
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli; D006602:Hexosyltransferases; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010455:Peptides / Q000378:metabolism; D014176:Protein Biosynthesis; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D009842:Oligopeptides; D010455:Peptides; C026146:N-acetylglucosamine-1-phosphate; C085706:FLAG peptide; D006602:Hexosyltransferases; C122139:RgpG protein, Streptococcus mutans; D000117:Acetylglucosamine