Identification of a DNA region from lactococcal phage sk1 protecting phage 712 from the abortive infection mechanism AbiF.
Bacteriophage 712 is a small isometric-headed phage which is sensitive to the lactococcal abortive infection mechanism AbiF. Its 29.6-kb DNA genome was characterized by restriction mapping and transcriptional analysis. Construction of a gene bank of lactococcal phage sk1, which is insensitive to the action of AbiF, in Lactococcus lactis containing AbiF resulted in the identification of a 324-bp DNA fragment which reduced the effect of the abortive infection mechanism on phage 712. Analysis of this region provided evidence that the action of AbiF is related to the cos ends of small isometric-headed phages. Sequence analysis of a 3.2-kb segment containing the middle operon and the cos ends of phage 712 genome allowed comparison of this part of the phage 712 genome with the equivalent sequences of four other small isometric-headed phages.
Rincé
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National Food Biotechnology Centre, University College, Cork, Ireland.
Tangney
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Fitzgerald
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GF
D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D005816:Genetic Complementation Test; D013294:Lactococcus lactis / Q000821:virology*; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017906:Siphoviridae / Q000235:genetics* / Q000502:physiology*; D014158:Transcription, Genetic
D004279:DNA, Viral