Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid.
We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line AC1-1. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, AC1-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and its underlying genetic mechanisms.
Frank
H G
HG
Department of Anatomy, Technical University of Aachen, Germany.
Gunawan
B
B
Ebeling-Stark
I
I
Schulten
H J
HJ
Funayama
H
H
Cremer
U
U
Huppertz
B
B
Gaus
G
G
Kaufmann
P
P
Füzesi
L
L
D002459:Cell Fusion; D002822:Choriocarcinoma / Q000235:genetics*; D002871:Chromosome Banding; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D011247:Pregnancy; D014327:Trophoblasts; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics*