Inhibition of Listeria monocytogenes by in situ produced and semipurified bacteriocins of Carnobacterium spp. on vacuum-packed, refrigerated cold-smoked salmon.
Listeria monocytogenes inhibition by Carnobacterium strains and crude bacteriocins on sterile and commercial vacuum-packed cold-smoked salmon stored at 4 degrees C and 8 degrees C was investigated. Carnobacterium piscicola V1 was bactericidal against L. monocytogenes at the two temperatures, whereas Carnobacterium divergens V41 presented a bacteriostatic effect. C. piscicola SF668 delayed L. monocytogenes growth at 8 degrees C and had a bacteriostatic effect at 4 degrees C. Listeria growth was not affected by a non-bacteriocin-producing C. piscicola. Crude extracts of piscicocins were bactericidal at 4 degrees C and 8 degrees C. Listeria growth was delayed by divercin V41 at 8 degrees C and was inhibited at 4 degrees C. Nisin delayed Listeria growth at 8 degrees C and was bacteriostatic at 4 degrees C. The present study demonstrates that L. monocytogenes growth could be prevented on vacuum-packed cold-smoked salmon by Carnobacterium and associated bacteriocins at chilled temperatures. Moreover, no product spoilage could be observed with the use of such bacteriocin-producing strains as demonstrated by good sensorial analyses and low biogenic amine production.
Duffes
F
F
ENITIAA, Laboratoire de Microbiologie, Nantes, France. duffes@labtechno.roazhon.inra.fr
Corre
C
C
Leroi
F
F
Dousset
X
X
Boyaval
P
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001430:Bacteriocins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology*; D015169:Colony Count, Microbial; D005511:Food Handling; D005516:Food Microbiology; D018857:Food Packaging; D005519:Food Preservation; D007777:Lactobacillaceae / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology; D012034:Refrigeration; D012474:Salmon / Q000382:microbiology*; D014618:Vacuum
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D009561:Nisin