Ox40-ligand has a critical costimulatory role in dendritic cell:T cell interactions.
The tumor necrosis factor family molecule Ox40-ligand (Ox40L) has been identified as a potential costimulatory molecule and also has been implicated in T cell homing and B cell activation. To ascertain the essential functions of Ox40L, we generated and characterized Ox40L-deficient mice. Mice lacking Ox40L exhibit an impaired contact hypersensitivity response, a dendritic cell-dependent T cell-mediated response, due to defects in T cell priming and cytokine production. In contrast, Ox40L-deficient mice do not have defects in T cell homing or humoral immune responses. In vitro, Ox40L-deficient dendritic cells are defective in costimulating T cell cytokine production. Thus, Ox40L has a critical costimulatory function in vitro and in vivo for dendritic cell:T cell interactions.
Chen
A I
AI
Immunology Research Division, Department of Pathology, Brigham and Women's Hospital and the Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
McAdam
A J
AJ
Buhlmann
J E
JE
Scott
S
S
Lupher
M L
ML
Greenfield
E A
EA
Baum
P R
PR
Fanslow
W C
WC
Calderhead
D M
DM
Freeman
G J
GJ
Sharpe
A H
AH
D016475:3T3 Cells; D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D053278:OX40 Ligand; D010047:Ovalbumin / Q000276:immunology; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D048069:Tumor Necrosis Factors
D000955:Antigens, T-Independent; D006241:Haptens; D008562:Membrane Glycoproteins; D053278:OX40 Ligand; D018124:Receptors, Tumor Necrosis Factor; C504586:Tnfsf4 protein, mouse; D048069:Tumor Necrosis Factors; C054401:trinitrophenyl keyhole limpet hemocyanin; C038600:trinitrophenyl-ovalbumin; D010047:Ovalbumin; D006433:Hemocyanins