title: Evaluation of a commercial ELISA for detecting serum antibody to Neospora caninum in cattle.
abstract: A commercially available serum antibody detection enzyme-linked immunosorbent assay for Neospora caninum in cattle was evaluated against an immunofluorescent antibody test (IFAT) by applying it to 397 sera from normal adult cattle, 352 sera from cattle which had recently aborted, and 422 sera from two herds which had a history of N caninum-associated abortions. It was evaluated in two laboratories and showed high reproducibility, repeatability and almost perfect or substantial agreement with the IFAT.
authors.affiliation: Veterinary Parasitology, Liverpool School of Tropical Medicine/Faculty of Veterinary Science, University of Liverpool.
authors.forename: D J
authors.initials: DJ
authors.lastname: Williams
authors.forename: H C
authors.initials: HC
authors.lastname: Davison
authors.forename: B
authors.initials: B
authors.lastname: Helmick
authors.forename: J
authors.initials: J
authors.lastname: McGarry
authors.forename: F
authors.initials: F
authors.lastname: Guy
authors.forename: A
authors.initials: A
authors.lastname: Otter
authors.forename: A J
authors.initials: AJ
authors.lastname: Trees
mesh_terms: D000034:Abortion, Veterinary / Q000469:parasitology*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D003048:Coccidiosis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D005260:Female; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006801:Humans; D018688:Neospora / Q000276:immunology*; D011247:Pregnancy; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan