Virulent strains of Helicobacter pylori demonstrate delayed phagocytosis and stimulate homotypic phagosome fusion in macrophages.
Helicobacter pylori colonizes the gastric epithelium of approximately 50% of the world's population and plays a causative role in the development of gastric and duodenal ulcers. H. pylori is phagocytosed by mononuclear phagocytes, but the internalized bacteria are not killed and the reasons for this host defense defect are unclear. We now show using immunofluorescence and electron microscopy that H. pylori employs an unusual mechanism to avoid phagocytic killing: delayed entry followed by homotypic phagosome fusion. Unopsonized type I H. pylori bound readily to macrophages and were internalized into actin-rich phagosomes after a lag of approximately 4 min. Although early (10 min) phagosomes contained single bacilli, H. pylori phagosomes coalesced over the next approximately 2 h. The resulting "megasomes" contained multiple viable organisms and were stable for 24 h. Phagosome-phagosome fusion required bacterial protein synthesis and intact host microtubules, and both chloramphenicol and nocodazole increased killing of intracellular H. pylori. Type II strains of H. pylori are less virulent and lack the cag pathogenicity island. In contrast to type I strains, type II H. pylori were rapidly ingested and killed by macrophages and did not stimulate megasome formation. Collectively, our data suggest that megasome formation is an important feature of H. pylori pathogenesis.
Allen
L A
LA
Department of Medicine, University of Iowa, Veterans Affairs Medical Center, Iowa City, Iowa 52242, USA. lee-ann-allen@uiowa.edu
Schlesinger
L S
LS
Kang
B
B
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000502:physiology; D002459:Cell Fusion; D005260:Female; D016480:Helicobacter pylori / Q000276:immunology / Q000472:pathogenicity*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D010587:Phagocytosis; D010588:Phagosomes / Q000276:immunology*; D014774:Virulence
D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori