Routine and sensitive method for determination of nicorandil in human plasma developed for liquid chromatography with ultraviolet and mass spectrometric detection.
A rapid and sensitive method using HPLC has been developed for the quantification of nicorandil (SG-75) in human plasma samples for routine bioequivalence studies. The sample preparation needs two liquid-liquid extractions, first with CH3Cl and HClO4 as denaturation reagent and second with addition of ethyl acetate and Na2CO3(aq). Detection wavelength was 256 nm. The obtained correlation coefficient for weighted linear curve in the range from 5.0 to 300 ng/ml was higher than 0.9950. The limit of quantitation (LOQ) was established at 5.0 ng/ml. The HPLC separation was accomplished on Nucleosil Phenyl (5 microm) stainless steel column within 7 min. The mixture of 0.01 M ammonium acetate buffer (pH 6.2) and acetonitrile 10:3 (v/v) was used as the mobile phase. The same separation method was examined on HPLC-MS system. Using this system, the LOQ was established at 1.0 ng/ml and the linearity was obtained in the range from 1.0 to 150 ng/ml.
Andrensek
S
S
National Institute of Chemistry, Laboratory for Food Chemistry, Ljubljana, Slovenia. samo.adrensek@ki.si
Smidovnik
A
A
Pecavar
A
A
Prosek
M
M
D000085:Acetates; D000097:Acetonitriles; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D020108:Nicorandil / Q000097:blood* / Q000493:pharmacokinetics; D011786:Quality Control; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014665:Vasodilator Agents / Q000097:blood* / Q000493:pharmacokinetics
D000085:Acetates; D000097:Acetonitriles; D002021:Buffers; D007202:Indicators and Reagents; D014665:Vasodilator Agents; D020108:Nicorandil; C018824:ammonium acetate; C032159:acetonitrile