Biologically active components against Drosophila melanogaster from Podophyllum hexandrum.
In the course of screening for novel naturally occurring insecticides from Chinese crude drugs, a dichloromethane extract of Podophyllum hexandrum was found to give an insecticidal activity against larvae of Drosophila melanogaster Meigen. From the extract, an insecticidal compound was isolated by bioassay-guided fractionation. The compound was identified as podophyllotoxin (1) by comparison of its spectroscopic characteristics with literature data. In bioassays for insecticidal activity, 1 showed a LC(50) value of 0.24 micromol/mL diet against larvae of D. melanogaster and a LD(50) value of 22 microg/adult against adults. Acetylpodophyllotoxin (1A), however showed slight insecticidal activity in both assays, indicating that the 4-hydroxyl group was an important function for enhanced activity of 1.
Miyazawa
M
M
Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University, Kowakae, Higashiosaka-shi, Osaka 577-8502, Japan.
Fukuyama
M
M
Yoshio
K
K
Kato
T
T
Ishikawa
Y
Y
D000818:Animals; D004330:Drosophila; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides / Q000302:isolation & purification*; D007814:Larva; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011034:Podophyllotoxin / Q000302:isolation & purification*; D011035:Podophyllum / Q000737:chemistry*; D013329:Structure-Activity Relationship
D007306:Insecticides; D010936:Plant Extracts; D011034:Podophyllotoxin