title: X-ray microanalytical and morphological observations of aqueous humor from cataract human eyes with and without diabetes mellitus.
abstract: This study was conducted to determine the elemental composition of the aqueous humor in cataract human eyes, with and without diabetes mellitus. The aqueous humor was obtained during cataract surgery from 11 patients with diabetes mellitus and 11 controls. Droplets of aqueous humor, after air-drying on carbon plates, were analyzed by X-ray microanalysis. Before surgery, the protein concentration in the anterior chamber was measured with a laser flare meter. Flare intensity was significantly higher in eyes with diabetes mellitus than in controls (p < 0.05). The X-ray microanalytical results showed that the relative sulfur content in the aqueous humor was significantly higher in the diabetic eyes than in the controls (p < 0.05). The content of elements representing the mobile ions in the aqueous humor, i.e., Na, Cl, K, and Ca was similar in both studied groups. We conclude from these data that the high sulfur content in aqueous humor of diabetic eyes may be related to a higher level of proteins found in the humor in these eyes.
authors.affiliation: Department of Medical Cell Biology, University of Uppsala, Sweden. Romuald.Wroblewski@medcellbiol.uu.se
authors.forename: R
authors.initials: R
authors.lastname: Wróblewski
authors.forename: A
authors.initials: A
authors.lastname: Zaczek
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000737:chemistry* / Q000166:cytology*; D002386:Cataract / Q000150:complications / Q000473:pathology*; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged