The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization.
Positioning of the Z ring at the midcell site in Escherichia coli is assured by the min system, which masks polar sites through topological regulation of MinC, an inhibitor of division. To study how MinC inhibits division, we have generated a MalE-MinC fusion that retains full biological activity. We find that MalE-MinC interacts with FtsZ and prevents polymerization without inhibiting FtsZ's GTPase activity. MalE-MinC19 has reduced ability to inhibit division, reduced affinity for FtsZ, and reduced ability to inhibit FtsZ polymerization. These results, along with MinC localization, suggest that MinC rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.
Hu
Z
Z
Department of Microbiology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Mukherjee
A
A
Pichoff
S
S
Lutkenhaus
J
J
D018528:ATP-Binding Cassette Transporters; D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002451:Cell Compartmentation; D002455:Cell Division; D003598:Cytoskeletal Proteins; D004926:Escherichia coli / Q000166:cytology*; D029968:Escherichia coli Proteins; D020558:GTP Phosphohydrolases / Q000378:metabolism; D058619:Maltose-Binding Proteins; D008954:Models, Biological; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D033902:Periplasmic Binding Proteins; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D029968:Escherichia coli Proteins; C071525:FtsZ protein, Bacteria; C461619:MalE protein, E coli; D058619:Maltose-Binding Proteins; C080466:MinC protein, Bacteria; D009004:Monosaccharide Transport Proteins; D033902:Periplasmic Binding Proteins; D011993:Recombinant Fusion Proteins; C461599:maltose transport system, E coli; C053139:sulA protein, E coli; D000251:Adenosine Triphosphatases; D020558:GTP Phosphohydrolases; C072069:MinD protein, E coli