Cloning and analysis of cDNAs encoding the hypusine-containing protein eIF5A of two lepidopteran insect species.
Eukaryotic initiation factor eIF5A is essential for cell viability and contains a characteristic post-translational modification of a specific lysine residue into a hypusine. cDNAs with similarity to eIF5A sequences were derived from Spodoptera exigua and S. frugiperda cDNA libraries. The deduced amino acid sequences are identical for both species and predict a protein with a molecular mass of 17.5 kDa. The Drosophila melanogaster eIF5A cDNA sequence was retrieved from the Drosophila EST Project. The predicted protein is 80% similar to Spodoptera eIF5A. A single eIF5A gene copy is present in the S. frugiperda genome, which is transcribed into four different transcripts. Infection of S. frugiperda cells with a baculovirus resulted in a strong decline of all four transcripts already at 12 h after infection. In contrast, the eIF5A protein was fairly stable up to 48 h post infection.
van Oers
M M
MM
Department of Molecular Cell Biology, University of Utrecht, The Netherlands. monique.vanoers@medew.viro.wau.nl
van Marwijk
M
M
Kwa
M S
MS
Vlak
J M
JM
Thomas
A A
AA
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D015870:Gene Expression; D008239:Lysine / Q000031:analogs & derivatives*; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D016601:RNA-Binding Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D018411:Spodoptera / Q000235:genetics* / Q000821:virology; D000097574:Eukaryotic Translation Initiation Factor 5A
D018076:DNA, Complementary; D008239:Lysine; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C100028:hypusine