title: An agonist anti-human CD40 monoclonal antibody that induces dendritic cell formation and maturation and inhibits proliferation of a myeloma cell line.
abstract: CD40, a 48-50 KD cell membrane molecule, member of the nerve growth factor receptor and tumor necrosis factor receptor superfamily, is an important costimulatory molecule during the immune response. Anti-CD40 monoclonal antibody (MAb) has been shown earlier to costimulate with IgM or phorbol esters resting B cells to proliferate, differentiate, secrete immunoglobulins, and switch isotype. Here we report on an agonistic mouse anti-human CD40 MAb 5C11. The specificity of this MAb was verified by flow cytometry, Western blotting, and competition with anti-CD40 MAb 89. We studied the effects of MAb 5C11 on a multimyeloma cell line, XG2, that expresses the CD40 antigen strongly and found that this MAb caused the homotypic aggregation of XG2, strongly suppressed XG2 proliferation, and led to its apoptosis after 24 hr of treatment. Interestingly, MAb 5C11 also triggered the generation, proliferation, and maturation of dendritic cells from peripheral blood monocytes, either by itself or in combination with GM-CSF and IL-4.
authors.affiliation: Department of Immunology, Suzhou Medical College, PR China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhou
authors.forename: J F
authors.initials: JF
authors.lastname: Wang
authors.forename: Y D
authors.initials: YD
authors.lastname: Wang
authors.forename: Y H
authors.initials: YH
authors.lastname: Qiu
authors.forename: J Z
authors.initials: JZ
authors.lastname: Pan
authors.forename: W
authors.initials: W
authors.lastname: Xie
authors.forename: L Y
authors.initials: LY
authors.lastname: Jiang
authors.forename: B
authors.initials: B
authors.lastname: Klein
authors.forename: X G
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology*; D017209:Apoptosis; D015153:Blotting, Western; D019013:CD40 Antigens / Q000276:immunology*; D002455:Cell Division; D003713:Dendritic Cells / Q000166:cytology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D019013:CD40 Antigens