title: Reduced flux through the purine biosynthetic pathway results in an increased requirement for coenzyme A in thiamine synthesis in Salmonella enterica serovar typhimurium.
abstract: Work presented here establishes a connection between cellular coenzyme A (CoA) levels and thiamine biosynthesis in Salmonella enterica serovar Typhimurium. Prior work showed that panE mutants (panE encodes ketopantoate reductase) had a conditional requirement for thiamine or pantothenate. Data presented herein show that the nutritional requirement of panE mutants for either thiamine or pantothenate is manifest only when flux through the purine biosynthetic pathway is reduced. Further, the data show that under the above conditions it is the lack of thiamine pyrophosphate, and not decreased CoA levels, that directly prevents growth.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, Madison 53706, USA.
authors.forename: M
authors.initials: M
authors.lastname: Frodyma
authors.forename: A
authors.initials: A
authors.lastname: Rubio
authors.forename: D M
authors.initials: DM
authors.lastname: Downs
mesh_terms: D000225:Adenine / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000235:genetics; D003065:Coenzyme A / Q000378:metabolism*; D003470:Culture Media; D009154:Mutation; D010205:Pantothenic Acid / Q000378:metabolism; D011687:Purines / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis*; D013835:Thiamine Pyrophosphate / Q000378:metabolism
chemical_list: D003470:Culture Media; D011687:Purines; D010205:Pantothenic Acid; D000429:Alcohol Oxidoreductases; C021934:2-dehydropantoate 2-reductase; D000225:Adenine; D013835:Thiamine Pyrophosphate; D003065:Coenzyme A; D013831:Thiamine