Rat brain proteins: two-dimensional protein database and variations in the expression level.
A two-dimensional database of rat brain proteins was constructed. Brain samples from newborn animals were analyzed by two-dimensional electrophoresis and the proteins were identified by matrix-assisted laser desorption/ionization mass spectrometry. The database comprises 210 different proteins, the majority of which are structural components, heat shock proteins and enzymes with various catalytic activities. Several minor differences in the expression level were detected, mainly of quantitative nature, which most likely represent allelic differences. The map may be useful in studies of neurological disorders in animal models of human diseases.
Fountoulakis
M
M
F. Hoffmann-La Roche, Pharmaceutical Research, Genomics Technologies, Basel, Switzerland. michael.fountoulakis@roche.com
Schuller
E
E
Hardmeier
R
R
Berndt
P
P
Lubec
G
G
D000818:Animals; D001923:Brain Chemistry; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009419:Nerve Tissue Proteins