Kinetics of prion protein accumulation in the CNS of mice with experimental scrapie.
The kinetics of PrP(Sc) and insoluble PrP accumulation in the spleens and brains of CD-1 mice were studied. The mice were inoculated intracerebrally with RML prions and euthanized at various times between inoculation and the onset of illness at approximately 130 days. Protease-resistant PrP(Sc), PrP 27-30, was first detected in brain by histoblotting 49 days after inoculation and by Western immunoblotting at 70 days. In spleen, PrP 27-30 was first detected by Western immunoblotting at 28 days after inoculation. Like PrP 27-30, substantial increases in detergent-insoluble PrP were first detected at 70 days after inoculation in brain and 28 days in spleen. In addition, a progressive increase in detergent-soluble PrP was detected beginning 70 days after inoculation. Further characterization of detergent soluble and insoluble PrP with respect to protease-sensitive PrP(Sc) and prion infectivity will be of considerable interest.
Tatzelt
J
J
Department of Neurology, University of California, San Francisco 94143-0518, USA.
Groth
D F
DF
Torchia
M
M
Prusiner
S B
SB
DeArmond
S J
SJ
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D018622:PrP 27-30 Protein / Q000378:metabolism; D018620:PrPSc Proteins / Q000378:metabolism; D011328:Prions / Q000378:metabolism*; D012608:Scrapie / Q000378:metabolism*; D012995:Solubility; D013154:Spleen / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution
D003902:Detergents; D018620:PrPSc Proteins; D011328:Prions; D018622:PrP 27-30 Protein