Transposon associated markers for the parasitic nematode Haemonchus contortus.
We have previously characterized a Tcl-like transposable element Hctcl, from the parasitic nematode Haemonchus contortus. Here we describe the genetic variation of Hctcl insertion sites in H. contortus populations differing in geographical origin, resistance to chemotherapeutics and level of inbreeding. Clear differences between populations were observed on Southern blots with a Hctcl-specific probe. Sequencing the 5'- or 3'-flanks of individual Hctcl integration sites allowed the design of PCR reactions between a Hctcl-specific primer and the flanking regions. This revealed a considerable variation of integration sites of Hctcl both within and between populations, although several integrations were shared by populations of different geographical origin. For four of the eight markers allele frequencies were shifted during selection for resistance to chemotherapeutics and/or inbreeding. For two positions both the 5' and 3' regions flanking Hctcl were isolated and PCR showed that for these two positions the variation of transposon associated markers between populations were indeed caused by variation in integration of Hctcl. For these two positions co-dominant markers were developed. These results indicate that Hctcl insertions may serve as genetic markers for H. contortus.
Hoekstra
R
R
Department of Molecular Recognition, Institute for Animal Science and Health (ID-Lelystad), The Netherlands. r.hoekstra@id.dlo.nl
Otsen
M
M
Tibben
J
J
Lenstra
J A
JA
Roos
M H
MH
D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology; D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D006189:Haemonchus / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D000969:Antinematodal Agents; D004251:DNA Transposable Elements; D018509:DNA, Helminth; D005819:Genetic Markers