title: [Use of high-density filters ("DNA arrays") for gene expression analysis in human tissues].
abstract: Most technical strategies for the analysis of gene expression in tissues are able to study only one protein or RNA product at the same time. A new recent method referred to as <<DNA array>> or <<high density filter>> is able to analyze simultaneously several hundreds of different genes. The DNA array is a nylon membrane on which are spotted equal amounts of cDNAs corresponding to different genes. This filter is hybridized with a <<complex>> probe synthesized with mRNA derived from the tissue analyzed. The result gives a global profile of gene expression within the tissue and allows quantitative and comparative analysis between different tissues or cell types.
authors.affiliation: U119 de l'INSERM, 27 Bd Lei Roure, 13009 Marseille.
authors.forename: S
authors.initials: S
authors.lastname: Oddou
authors.forename: E
authors.initials: E
authors.lastname: Devilard
authors.forename: J P
authors.initials: JP
authors.lastname: Dales
authors.forename: J
authors.initials: J
authors.lastname: Hassoun
authors.forename: F
authors.initials: F
authors.lastname: Birg
authors.forename: L
authors.initials: L
authors.lastname: Xerri
mesh_terms: D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D010336:Pathology / Q000295:instrumentation / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D012333:RNA, Messenger