title: Translocation of group 1 capsular polysaccharide to the surface of Escherichia coli requires a multimeric complex in the outer membrane.
abstract: Surface expression of the group 1 K30 capsular polysaccharide of Escherichia coli strain E69 (O9a:K30) requires Wza(K30), a member of the outer membrane auxiliary (OMA) protein family. A mutation in wza(K30) severely restricts the formation of the K30 capsular structure on the cell surface, but does not interfere with the biosynthesis or polymerization of the K30 repeat unit. Here we show that Wza(K30) is a surface-exposed outer membrane lipoprotein. Wza(K30) multimers form ring-like structures in the outer membrane that are reminiscent of the secretins of type II and III protein translocation systems. We propose that Wza(K30) forms an outer membrane pore through which the K30-capsular antigen is translocated. This is the first evidence of a potential mechanism for translocation of high molecular weight polysaccharide across the outer membrane. The broad distribution of the OMA protein family suggests a similar process for polysaccharide export in diverse Gram-negative bacteria.
authors.affiliation: Department of Microbiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
authors.forename: J
authors.initials: J
authors.lastname: Drummelsmith
authors.forename: C
authors.initials: C
authors.lastname: Whitfield
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008854:Microscopy, Electron; D011817:Rabbits; D013499:Surface Properties
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C030659:K antigens; C404158:Wza protein, E coli