Transfer of some carboxylic acids in the olfactory system following intranasal administration.
The uptake of [14C]benzoic acid, 4-chloro[14C]benzoic acid, [3H]phthalic acid and [14C]salicylic acid in the nasal passages and brain was determined following a unilateral intranasal instillation in mice. An uptake of radioactivity from the nasal mucosa to the ipsilateral olfactory bulb was observed up to 4 h after administration following intranasal instillation of these carboxylic acids whereas the level was low in the contralateral olfactory bulb. Autoradiography of mice given [14C]benzoic acid and [14C]salicylic acid by intranasal instillation showed a preferential localization of radioactivity in the axonal and glomerular layer of the olfactory bulb 1 h after the administration. Four hours after administration the radioactivity was present as a gradient from the axonal layer towards the center of the olfactory bulb. Pretreatment of mice with a compound known to damage the olfactory neuroepithelium resulted in a decreased uptake of [14C]benzoic acid in the olfactory bulb. Thin layer chromatography of supernatants from the ipsilateral olfactory bulbs of mice given [14C]benzoic acid by nasal instillation indicated that the radioactivity in the bulbs represented unchanged compound. These results suggest that there is a transfer of some aromatic carboxylic acids in the olfactory pathways.
Eriksson
C
C
Department of Pharmacology and Toxicology, Swedish University of Agricultural Sciences, Uppsala.
Bergman
U
U
Franzén
A
A
Sjöblom
M
M
Brittebo
E B
EB
D000281:Administration, Intranasal; D000818:Animals; D001345:Autoradiography; D019817:Benzoic Acid / Q000493:pharmacokinetics; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002264:Carboxylic Acids / Q000493:pharmacokinetics*; D002723:Chlorobenzoates / Q000493:pharmacokinetics; D002855:Chromatography, Thin Layer; D005260:Female; D051379:Mice; D009297:Nasal Mucosa / Q000378:metabolism* / Q000473:pathology; D009830:Olfactory Bulb / Q000378:metabolism* / Q000473:pathology; D010795:Phthalic Acids / Q000493:pharmacokinetics; D020156:Salicylic Acid / Q000493:pharmacokinetics; D013997:Time Factors
D002264:Carboxylic Acids; D002723:Chlorobenzoates; D010795:Phthalic Acids; C032279:phthalic acid; D019817:Benzoic Acid; D020156:Salicylic Acid