Evaluation of the quantiplex human immunodeficiency virus type 1 RNA 3.0 assay in a tertiary-care center.
The Quantiplex human immunodeficiency virus type 1 RNA 3.0 Assay (bDNA) (Bayer Diagnostics, Walpole, Mass.) produced linear and reproducible (intra-assay and interassay) results over its quantification range of 50 to 500,000 copies/ml of plasma with 96% specificity. A threefold or 0.5-log(10) change or greater was clinically significant for serial patient samples.
Gale
H
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Infectious Diseases Section, Medical Service, Veterans Affairs Medical Center, Washington, D.C 20422, USA. gale.howard@washington.va.gov
D000554:Ambulatory Care Facilities; D005898:Glass / Q000737:chemistry; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D010969:Plastics / Q000737:chemistry; D012367:RNA, Viral / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*
D010969:Plastics; D012367:RNA, Viral