title: DNA sequencing in a monolithic microchannel device.
abstract: We present 50 cm long microchannels in a monolithic device for high resolution, long read-length DNA sequencing. These devices were fabricated and bonded in borofloat glass using unconventional photolithography techniques with 48-188 independent, straight microchannels. The microchannel DNA separation was tested with POP-6 polymer and a DNA sequencing ladder separated at room temperature and 200 V/cm. Single-base resolution greater than 600 bases was achieved and the sequence base called to 640 bases with 98% accuracy. Under the same experimental conditions, the performance of the microchip was identical to a fused-silica capillary with similar cross-sectional area.
authors.affiliation: PE Biosystems, Foster City, CA 94404, USA.
authors.forename: C
authors.initials: C
authors.lastname: Backhouse
authors.forename: M
authors.initials: M
authors.lastname: Caamano
authors.forename: F
authors.initials: F
authors.lastname: Oaks
authors.forename: E
authors.initials: E
authors.lastname: Nordman
authors.forename: A
authors.initials: A
authors.lastname: Carrillo
authors.forename: B
authors.initials: B
authors.lastname: Johnson
authors.forename: S
authors.initials: S
authors.lastname: Bay
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods*; D004864:Equipment and Supplies; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA