Detection of genomic variability in different populations of the cattle tick Boophilus microplus in southern Brazil.
DNA from seven isolates of the cattle tick Boophilus microplus was analyzed by restriction fragment length polymorphism (RFLP). Three different cDNA clones, named P-9, P-25 and CP-12, isolated from a B. microplus cDNA library, were used as DNA probes. DNA sequences of P-9 have high similarity to ribosomal genes, whereas P-25 does not show significant homology with known sequences within databases. CP-12 is a cDNA clone encoding a cysteine endopeptidase gene. A limited degree of polymorphism was detected with P-9 and P-25, while CP-12 showed a different pattern of bands for each tick isolate. These findings suggest the existence of a complex genotypic diversity of the tick B. microplus population in endemic regions.
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Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
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da Silva
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Richter
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Ozaki
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D000818:Animals; D015139:Blotting, Southern; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D004247:DNA / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry; D005260:Female; D015723:Gene Library; D014644:Genetic Variation / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D013987:Ticks / Q000737:chemistry / Q000235:genetics*
D015342:DNA Probes; D012313:RNA; D004247:DNA; D015246:Deoxyribonuclease EcoRI