Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments.
In experiments with proteins of molecular weights around 100 kDa the implementation of [15N,1H]-TROSY-elements in [15N]-constant-time triple resonance experiments yields sensitivity enhancements of one to two orders of magnitude. An additional gain of 10 to 20% may be obtained with the use of 'sensitivity enhancement elements'. This paper describes a novel sensitivity enhancement scheme which is based on concatenation of the 13C alpha-->15N magnetization transfer with the ST2-PT element, and which enables proper TROSY selection of the 15N multiplet components.
Salzmann
M
M
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, Zürich, Switzerland.
Wider
G
G
Pervushin
K
K
Wüthrich
K
K
D000446:Aldehyde-Lyases / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D008280:Magnetics; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000201:enzymology
D001426:Bacterial Proteins; D000446:Aldehyde-Lyases; C020044:dihydroneopterin aldolase