Prognostic significance of DNA image cytophotometry for osteosarcoma.
OBJECTIVE
To investigate the prognostic significance of DNA image cytophotometric data.


METHODS
Twenty-six osteosarcomas in patients without lung metastases were investigated for several cytophotometric data. In 24 cases, these data were correlated with the clinical course of the patients to assess the prognostic value of nuclear DNA content in osteosarcomas.


RESULTS
Of all osteosarcomas, 96% showed aneuploid DNA content. Patients with tumors having a 2c deviation index (2cDI) of 12.00, DNA malignancy grade (DNA-MG) of 2.0, a mean DNA content (MDC) of 4.95 c, DNA index (DI) of 1.75 or mean nuclear area (MNA) of 130 microns 2 had a significantly lower overall survival rate as compared to those with lower values (P < .05).


CONCLUSIONS
Image cytophotometric features, such as 2cDI, DNA-MG, MDC, DI and MNA, are of prognostic value in patients with osteosarcoma and free of lung metastases.
Neuburger
M
M
Reference Center of Bone Diseases, University of Freiburg, Germany.
Xia
Y
Y
Herget
G W
GW
Huang
Y
Y
Adler
C P
CP
D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D008175:Lung Neoplasms / Q000235:genetics / Q000401:mortality / Q000556:secondary; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality; D011237:Predictive Value of Tests; D011379:Prognosis; D016019:Survival Analysis
D004273:DNA, Neoplasm