title: Use of mitomycin C for maintaining myringotomy patency.
abstract: The purpose of this study was to determine whether topical mitomycin C could be used to prolong the patency of a myringotomy site in the absence of a ventilation tube. Mitomycin C is an antineoplastic agent used to maintain a patent trabeculectomy site in patients with glaucoma. The KTP laser was used to create bilateral myringotomies 0. 4 mm in diameter in 28 chicks (Gallus domesticus). Mitomycin C was applied to the right myringotomy site, and sterile water, used as a control, was applied to the left myringotomy site. The animals were divided into 2 groups with group A treated with freshly prepared mitomycin C and group B treated with 2-week-old mitomycin C. The patency rates of the experimental ears of groups A and B were 80% and 70%, respectively, 15 days after surgery and 33% and 7.7%, respectively, at 30 days. All myringotomy sites in the control ears were healed within 5 days. Using a logistic regression model for repeated measures, we determined the odds ratio between the experimental and control myringotomies of the combined groups to be 149.1 (P = 0.0001). We conclude that mitomycin C had a significant effect in prolonging the patency of myringotomies in chick tympanic membranes.
authors.affiliation: Division of Otolaryngology, Department of Surgery, University of Missouri School of Medicine, Columbia, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Estrem
authors.forename: R N
authors.initials: RN
authors.lastname: Vanleeuwen
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D002645:Chickens; D053685:Laser Therapy; D016015:Logistic Models; D008876:Middle Ear Ventilation / Q000379:methods; D016685:Mitomycin / Q000008:administration & dosage*; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage*; D014432:Tympanic Membrane / Q000601:surgery*; D014945:Wound Healing
chemical_list: D000903:Antibiotics, Antineoplastic; D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin