title: Complete amino acid sequence of Mytilus anterior byssus retractor paramyosin and its putative phosphorylation site.
abstract: A cDNA encoding the full-length paramyosin molecule was cloned from the mussel Mytilus galloprovincialis, a species closely related to Mytilus edulis. It contained 3,497 nucleotides (nt), with 79 and 826 nt for the 5' and 3' non-coding regions, respectively. The coding region was composed of 2,592 nt for 864 amino acid residues, a size typical of paramyosin. While genomic DNA digests with either HindIII or PstI exhibited a single band when hybridized with a SacI fragment of paramyosin cDNA, the digests with either EcoRV or EcoRI showed two bands, suggesting that the mussel has at least two genes encoding paramyosin. The mRNAs encoding paramyosin were most abundant in muscle tissues from byssus retractor and adductor muscles. Only traces of paramyosin transcripts were found in the tissue of foot, gill, inner mantle, and outer mantle. The same phosphorylatable peptide previously reported for paramyosin from the bivalve Mercenaria mercenaria, Ser-Arg-Ser-Met-Ser(P)-Val-Ser-Arg (Watabe et al. 1989. Comp Biochem Physiol 94B:813-821) was found in the C-terminal non-helical part of this Mytilus paramyosin. We predict that this particular paramyosin has a coiled-coil structure composed of two alpha-helices that show the heptad repeats (a-b-c-d-e-f-g) with further 28-amino acid repeat zones, where a and d tend to be occupied by nonpolar residues.
authors.affiliation: Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan. awatanbe@mail.ecc.u-tokyo.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Watabe
authors.forename: K
authors.initials: K
authors.lastname: Iwasaki
authors.forename: D
authors.initials: D
authors.lastname: Funabara
authors.forename: Y
authors.initials: Y
authors.lastname: Hirayama
authors.forename: M
authors.initials: M
authors.lastname: Nakaya
authors.forename: K
authors.initials: K
authors.lastname: Kikuchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D049872:Bivalvia / Q000378:metabolism*; D015152:Blotting, Northern / Q000662:veterinary; D015139:Blotting, Southern / Q000662:veterinary; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009119:Muscle Contraction; D009126:Muscle Relaxation; D010766:Phosphorylation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014335:Tropomyosin / Q000737:chemistry* / Q000235:genetics
chemical_list: D014335:Tropomyosin