title: Characterization of antitumor immunization to a defined melanoma antigen using genetically engineered murine dendritic cells.
abstract: A murine model of dendritic cell (DC)-based genetic immunization to a defined human melanoma antigen (Ag), MART-1/Melan-A (MART-1), was developed. The MART-1 gene was stably transfected into the nonimmunogenic mouse fibrosarcoma cell line NFSA that is syngeneic in C3Hf/Sem/Kam (C3H, H-2k) mice to generate the NFSA(MART1) cell line. In vivo protection from a lethal NFSA(MART1) tumor challenge could be generated by DCs transduced with a recombinant adenovirus (AdV) vector expressing MART-1 (AdVMART1). This model has the following characteristics: (a) immunological specificity and memory, (b) comparable protection for varying transduction multiplicities of infection, cell doses, and sites of DC inoculation but, interestingly, worse protection with increasing numbers of vaccinations, (c) the ability to treat small established tumors, (d) an absolute requirement for CD8 and CD4 T cells, (e) generation of MART-1-specific splenic cytotoxic T lymphocytes, and (f) up-regulation of both T helper type 1 and T helper type 2 cytokines. Genetically engineered DCs presenting defined tumor Ags represent an attractive method to generate effective immune responses.
authors.affiliation: Division of Surgical Oncology, University of California, Los Angeles 90095, USA.
authors.forename: A
authors.initials: A
authors.lastname: Ribas
authors.forename: L H
authors.initials: LH
authors.lastname: Butterfield
authors.forename: W H
authors.initials: WH
authors.lastname: McBride
authors.forename: V B
authors.initials: VB
authors.lastname: Dissette
authors.forename: A
authors.initials: A
authors.lastname: Koh
authors.forename: C M
authors.initials: CM
authors.lastname: Vollmer
authors.forename: B
authors.initials: B
authors.lastname: Hu
authors.forename: A Y
authors.initials: AY
authors.lastname: Chen
authors.forename: J A
authors.initials: JA
authors.lastname: Glaspy
authors.forename: J S
authors.initials: JS
authors.lastname: Economou
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D008175:Lung Neoplasms / Q000556:secondary / Q000628:therapy; D058965:MART-1 Antigen; D008546:Melanoma, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008809:Mice, Inbred C3H; D009363:Neoplasm Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000951:Antigens, Neoplasm; D058965:MART-1 Antigen; C548667:MLANA protein, human; C548668:Mlana protein, mouse; D009363:Neoplasm Proteins