Effect of anticancer drugs, metals and antioxidants on cytotoxic activity of epigallocatechin gallate.
Various modulation factors for the cytotoxic action of epigallocatechin gallate (EGCG) against two human oral tumor cell lines (HSC-2, HSG) were investigated. Three anticancer drugs (tamoxifen, sulindac, doxorubicin), two metals (CuCl2, FeCl3) and two antioxidants (sodium ascorbate, tiopronin) did not significantly affect the cytotoxic activity of EGCG, Catalase and N-acetyl-L-cysteine only marginally reduced the cytotoxic activity of EGCG. On the other hand, CoCl2 significantly protected the cell injury induced by EGCG. This suggests that the site of EGCG action might be intracellular rather than extracellular. Possible involvement of the expression of transcription factor (s) for EGCG-induced cytotoxicity is discussed.
Ishino
A
A
Department of Dental Pharmacology, Meikai University School of Dentistry, Saitama, Japan.
Mita
S
S
Watanabe
S
S
Sakagami
H
H
D000111:Acetylcysteine / Q000494:pharmacology; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002712:Chlorides; D003035:Cobalt / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D004347:Drug Interactions; D005290:Ferric Compounds / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D009062:Mouth Neoplasms / Q000473:pathology*; D012468:Salivary Gland Neoplasms / Q000473:pathology; D013467:Sulindac / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology; D008625:Tiopronin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D016588:Anticarcinogenic Agents; D000970:Antineoplastic Agents; D002712:Chlorides; D016861:Cyclooxygenase Inhibitors; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D013629:Tamoxifen; D013467:Sulindac; D003035:Cobalt; D003300:Copper; D004317:Doxorubicin; D002392:Catechin; C045651:epigallocatechin gallate; D008625:Tiopronin; C018021:cobaltous chloride; D001205:Ascorbic Acid; C029892:cupric chloride; C024555:ferric chloride; D000111:Acetylcysteine