Forces acting on particle-enhanced immunoassays.
The aim of the present work is to study the role of the different forces involved in the agglutination of immuno gamma-globulin (IgG) covered latex particles due to antigen-antibody reaction. An experimental investigation on the adsorption of IgG molecules on three latexes with different surface charge densities is described. Photon correlation spectroscopy was used to determine the hydrodynamic layer thickness of the IgG molecules adsorbed on the latexes. In order to get an insight into the forces acting between two antibody-covered particles approaching each other, the colloidal stability and immunoreactivity of these biocomplexes were studied. They can be stabilized by electrostatic or hydration forces. The immunological agglutination of IgG-immobilized latex particles due to the addition of the antigen was quantified through scattered light intensity measurements. The immunoresponse increases with ionic strength of the medium until a maximum value is achieved. Above this maximum, the immunoreactivity decreases.
Molina-Bolívar
J A
JA
Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Spain.
Galisteo-González
F
F
Ortega-Vinuesa
J L
JL
Schmitt
A
A
Hidalgo-Alvarez
R
R
D000327:Adsorption; D000818:Animals; D000937:Antigen-Antibody Reactions; D002021:Buffers; D002097:C-Reactive Protein / Q000276:immunology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003102:Colloids; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D007840:Latex / Q000138:chemical synthesis / Q000737:chemistry*; D007841:Latex Fixation Tests; D008863:Microspheres; D009391:Nephelometry and Turbidimetry; D009994:Osmolar Concentration; D011817:Rabbits; D012680:Sensitivity and Specificity; D055672:Static Electricity; D013499:Surface Properties
D002021:Buffers; D003102:Colloids; D007074:Immunoglobulin G; D007840:Latex; D002097:C-Reactive Protein