Relative affinity and specificity of monoclonal antibodies against prostate-specific antigen.
The relative affinities of a panel of antibodies submitted to the ISOBM TD-3 PSA Workshop were determined by direct-binding ELISA. The Workshop antibodies were also tested for reactivity with the prostate-specific antigen alpha1-antichymotrypsin complex (PSA-ACT) and cross-reactivity with porcine pancreatic kallikrein. There was a wide range of affinities observed for the panel of antibodies. Twelve antibodies failed to react with the PSA-ACT complex, and 1 antibody was found to recognize an epitope on ACT but not on PSA. Only 2 antibodies were found to react with porcine pancreatic kallikrein, a protein with 64% sequence homology with human glandular kallikrein-2 (hK2).
Jette
D C
DC
Faculty of Pharmacy and Pharmaceutical Studies, University of Alberta, Edmonton, Canada.
Kreutz
F T
FT
Suresh
M R
MR
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D007118:Immunoassay; D017430:Prostate-Specific Antigen / Q000276:immunology*; D014407:Tumor Cells, Cultured; D000514:alpha 1-Antichymotrypsin / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000514:alpha 1-Antichymotrypsin; D017430:Prostate-Specific Antigen