Reactivity of 77 antibodies to prostate-specific antigen with isoenzymes and complexes of prostate-specific antigen.
Seventy-seven antibodies submitted to the ISOBM TD-3 PSA Workshop (TD-3.1 and TD-3.2) were characterized by measuring their reactivity with isoenzymes of free prostate-specific antigen (PSA), PSA complexed to alpha1-antichymotrypsin (PSA-ACT) and alpha1-proteinase inhibitor (PSA-API). Antibodies were classified into 15 distinct groups according to their reaction profiles with the various isoenzymes. Some antibodies recognizing both free and complexed PSA were inaccurate in measuring total PSA. Eight of the 9 free PSA-specific antibodies cross-reacted more with PSA-API than with PSA-ACT, while 1 antibody reacted less with PSA-API than PSA-ACT. From the panel of antibodies 39 reacted with both free and complexed PSA and were classified as total PSA antibodies.
Leinonen
J
J
Department of Clinical Chemistry, Helsinki University Central Hospital, Finland. jari.leinonen@huch.fi
Zhang
W M
WM
Paus
E
E
Stenman
U H
UH
D000911:Antibodies, Monoclonal / Q000145:classification / Q000378:metabolism*; D003429:Cross Reactions; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes; D017430:Prostate-Specific Antigen / Q000276:immunology*; D012661:Semen / Q000378:metabolism; D000514:alpha 1-Antichymotrypsin / Q000276:immunology; D000515:alpha 1-Antitrypsin / Q000276:immunology
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D017430:Prostate-Specific Antigen