Medium-chain triglycerides modulate ileitis induced by trinitrobenzene sulfonic acid.
BACKGROUND
It is important to develop an appropriate animal model for further investigation into inflammatory bowel disease (IBD). We therefore investigated a trinitrobenzene sulfonic acid (TNBS) ileitis model. Dietary fat in Crohn's disease is still a controversial risk factor for IBD. We therefore also studied the effects of medium-chain triglycerides (MCT) and long-chain triglycerides (LCT) on TNBS ileitis.


RESULTS
An intraileal injection of TNBS induced ulceration and inflammation with thickening of the intestinal wall, which were characterized histologically by infiltration of polymorphic nuclear leucocytes and by granuloma formation. The mucosal damage score and serum sialic acid levels reached their highest 7 days after the TNBS injection and then gradually decreased. The mucosal damage series in the MCT group was significantly lower than in the LCT group, and levels of tumour necrosis factor-alpha (TNF-alpha) and leukotriene B4 (LTB4) tended to be lower in the MCT group.


CONCLUSIONS
These results suggested that TNBS enteritis might be useful as an IBD animal model and that MCT modulates intestinal inflammation and is less damaging than LCT.
Tsujikawa
T
T
The Second Department of Internal Medicine, Shiga University of Medical Science, Otsu, Japan. tujikawa@belle.shiga-med.ac.jp
Ohta
N
N
Nakamura
T
T
Satoh
J
J
Uda
K
K
Ihara
T
T
Okamoto
T
T
Araki
Y
Y
Andoh
A
A
Sasaki
M
M
Fujiyama
Y
Y
Bamba
T
T
D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D007079:Ileitis / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007975:Leukotriene B4 / Q000378:metabolism; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D014280:Triglycerides / Q000737:chemistry / Q000627:therapeutic use*; D014302:Trinitrobenzenesulfonic Acid; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D011458:Prostaglandins E; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D007975:Leukotriene B4; D014302:Trinitrobenzenesulfonic Acid; D009955:Ornithine Decarboxylase