Employing target modifications for the investigation of liquid infrared matrix-assisted laser desorption/ionization mass spectrometry.
We present a new target modification for commercial ion sources to facilitate the use of liquid matrices such as glycerol and lactic acid in infrared matrix-assisted laser desorption/ionization (IR-MALDI). Benefits of this modification include increased sensitivity compared with the unmodified commercial target, mass resolution comparable to the best achievable with conventional IR-MALDI or UV-MALDI, and decreased sample volatility leading to an analysis time extended up to fivefold compared with using conventional target designs (up to 1 hour using volumes of only 250 nL).
Cramer
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Ludwig Institute for Cancer Research, 91 Riding House Street, London W1P 8BT, UK. rainer@ludwig.ucl.ac.uk
Burlingame
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D003574:Cytochrome c Group / Q000737:chemistry; D005990:Glycerol; D007328:Insulin / Q000737:chemistry; D019344:Lactic Acid; D010446:Peptide Fragments / Q000737:chemistry; D012680:Sensitivity and Specificity; D012996:Solutions; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014835:Volatilization; D000509:alpha-Fetoproteins / Q000737:chemistry
D003574:Cytochrome c Group; D007328:Insulin; D010446:Peptide Fragments; D012996:Solutions; D000509:alpha-Fetoproteins; D019344:Lactic Acid; D005990:Glycerol