Importance of nitric oxide in the regulation of burn oedema, proteinuria and urine output.
Burn injuries trigger a pronounced inflammatory response in the burned skin, resulting in oedema formation and impaired circulation. This response involves activation of the nitric oxide (NO) synthetic pathway, which could play a key role in the complex hemodynamic and hemostatic changes occurring as a result of a burn trauma. The results presented in full-thickness skin burns of rats show that the NO-precursor, L-arginine (n = 10), inhibit burn-induced plasma extravasation as compared to saline-treated burned controls (n = 10) (p<0.001) to a level not significantly different from nonburned animals. Administration of the NO-synthase inhibitor. NG-nitro-L-arginine (L-NNA) (n = 10), did not significantly influence burn extravasation compared to burned controls. Accumulated urine volume 90 min post-burn increased ten-fold in burned animals treated with L-arginine compared to saline-treated burned controls (p<0.001) and nonburned animals (p<0.001), while L-NNA had no significant effect on diuresis. A significantly increased proteinuria occurred in L-arginine treated burned animals as compared to burned controls and nonburned controls (p<0.001), whereas L-NNA did not significantly influence the leakage of protein in the urine. Activation of NO synthesis significantly suppresses burn edema and strongly increases diuresis along with increased proteinuria.
Lindblom
L
L
Department of Physiology and Pharmacology. Göteborg University, Sweden.
Cassuto
J
J
Yregård
L
L
Mattsson
U
U
Tarnow
P
P
Sinclair
R
R
D000418:Albumins; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002056:Burns / Q000150:complications*; D004231:Diuresis / Q000187:drug effects; D004487:Edema / Q000209:etiology* / Q000517:prevention & control; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005070:Evans Blue; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D011507:Proteinuria / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry; D014556:Urine / Q000502:physiology*
D000418:Albumins; D004791:Enzyme Inhibitors; D019335:Nitroarginine; D009569:Nitric Oxide; D005070:Evans Blue; D001120:Arginine; D019001:Nitric Oxide Synthase