title: Cardiochiles nigriceps polydnavirus: molecular characterization and gene expression in parasitized Heliothis virescens larvae.
abstract: Cardiochiles nigriceps Viereck is an endophagous parasitoid of larval stages of the tobacco budworm, Heliothis virescens (F.). This hymenopteran parasitoid, belonging to the family Braconidae, is associated with a polydnavirus (CnPDV), injected at ovi-position along with the egg. The infection of various tissues by CnPDV determines the suppression of the host immune system and the developmental arrest of mature host larvae. In this study, CnPDV has been characterized at the structural and molecular level. The negatively stained nucleocapsids show evident 'end structures' and a tail-like appendage. The CnPDV genome is typically segmented, with circular dsDNA molecules, ranging in size from 2.5 kb to more than 23 kb. The early expression pattern of CnPDV in parasitized hosts has been analysed and viral clones, genomic and cDNAs, identifying genes expressed within 48 h after parasitization have been isolated. The molecular organization of one of these genes, named CnPDV1, and its putative protein product have been determined. Significant sequence homologies with other known proteins were not detected. In situ hybridization experiments indicated that this gene is expressed in the prothoracic glands of parasitized host mature larvae. A functional analysis of CnPDV1 gene product is required to assess its possible role in the regulation of parasitoid-induced alterations of host larvae.
authors.affiliation: Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Università della Basilicata, Potenza, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Varricchio
authors.forename: P
authors.initials: P
authors.lastname: Falabella
authors.forename: R
authors.initials: R
authors.lastname: Sordetti
authors.forename: F
authors.initials: F
authors.lastname: Graziani
authors.forename: C
authors.initials: C
authors.lastname: Malva
authors.forename: F
authors.initials: F
authors.lastname: Pennacchio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D015870:Gene Expression; D005814:Genes, Viral; D016679:Genome, Viral; D007814:Larva / Q000469:parasitology; D008969:Molecular Sequence Data; D009036:Moths / Q000469:parasitology*; D018071:Polydnaviridae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D014863:Wasps / Q000821:virology*
chemical_list: D018076:DNA, Complementary; D014764:Viral Proteins; C403230:protein 1, Cardiochiles nigriceps polydnavirus