title: Efficacy of Ki-67 antigen staining in Papanicolaou (Pap) smears in post-menopausal women with atypia--an audit.
abstract: In cervical smears from post-menopausal women with mucosal atrophy it can be difficult to distinguish atrophic epithelial cell groups from neoplastic cell groups on cytomorphological criteria only. The consequence of post-menopausal atypia is that the woman is referred for a repeat smear after local oestrogen treatment or for colposcopy. We investigated whether immunocytochemical expression of Ki-67 (MIB-1) on the primary Papanicolaou-stained smear could be of any diagnostic help. Our data showed that negative Ki-67 expression is a very reliable indicator of a normal atrophic cell pattern, and by using this method on the original smear we were able to reduce the false-positive cytologic diagnoses by 86%.
authors.affiliation: Department of Pathology, Odense University Hospital, Denmark.
authors.forename: D
authors.initials: D
authors.lastname: Ejersbo
authors.forename: H A
authors.initials: HA
authors.lastname: Jensen
authors.forename: B
authors.initials: B
authors.lastname: Hølund
mesh_terms: D000328:Adult; D000368:Aged; D001284:Atrophy; D002278:Carcinoma in Situ / Q000473:pathology*; D002584:Cervix Uteri / Q000166:cytology / Q000473:pathology*; D003218:Condylomata Acuminata / Q000473:pathology; D003718:Denmark; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008875:Middle Aged; D009092:Mucous Membrane / Q000473:pathology; D065006:Papanicolaou Test; D017698:Postmenopause; D011785:Quality Assurance, Health Care; D015203:Reproducibility of Results; D012189:Retrospective Studies; D002577:Uterine Cervical Diseases / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D014626:Vaginal Smears / Q000592:standards*
chemical_list: D019394:Ki-67 Antigen