Effects of San'o-shashin-to on the antioxidative mechanism in spontaneous familial hypercholesterolaemic rabbits.
We investigated the effects of the Kampo medicine San'o-shashin-to which showed strong antioxidative effects in vitro on antioxidative mechanism in Kurosawa and Kusanagi- hypercholesterolaemic (KHC) rabbits, which are a good model of spontaneous familial hypercholesterolaemia. A 1-g kg(-1)dose of San'o-shashin-to was administered orally for 24 weeks and changes in plasma lipids, low-density lipoprotein (LDL) oxidation and redox dynamics of alpha-tocopherol in plasma and erythrocyte membrane were measured in a control group and San'o-shashin-to-treated group. A significant prolongation of the lag time was found in the 12th and 24th week of the experiment in San'o-shashin-to-treated group, indicating antioxidative effects on LDL. At the end of the administration period, the alpha-tocopherolquinone/alpha-tocopherol ratio in erythrocyte membrane, which reflects the utilization rate of alpha-tocopherol was significantly higher in San'o-shashin-to-treated group. There were no significant differences in the two groups with regards to plasma lipids. These results indicate that San'o-shashin-to had antioxidative effects on LDL in vivo and beneficial effects on redox dynamics of alpha-tocopherol, which is the strongest endogenous antioxidant.
Iijima
O T
OT
Department of Pharmacology and Intractable Diseases Research Center (Division of Drug Research and Development), Tokyo Medical University, Shinjuku-ku, Tokyo, 160-8402, Japan.
Takeda
H
H
Matsumiya
T
T
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001599:Berberine; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006937:Hypercholesterolemia / Q000235:genetics* / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D010936:Plant Extracts; D011817:Rabbits; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D008055:Lipids; D008077:Lipoproteins, LDL; D010936:Plant Extracts; C062798:sannoshashinto; D001599:Berberine; D014810:Vitamin E