Identification and characterization of a cystathionine beta/gamma-lyase from Lactococcus lactis ssp. cremoris MG1363.
This paper describes the nucleotide sequence of a gene encoding cystathionine beta/gamma-lyase from Lactococcus lactis ssp. cremoris MG1363, its overexpression in Escherichia coli and some functional characteristics of the purified recombinant protein.
Dobric
N
N
Department of Biochemistry and Molecular Biology, The University of Melbourne, Parkville, Vic., Australia.
Limsowtin
G K
GK
Hillier
A J
AJ
Dudman
N P
NP
Davidson
B E
BE
D000595:Amino Acid Sequence; D002611:Cheese / Q000382:microbiology; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003542:Cystathionine gamma-Lyase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D005798:Genes, Bacterial; D006710:Homocysteine / Q000378:metabolism; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity
D011994:Recombinant Proteins; D006710:Homocysteine; D003542:Cystathionine gamma-Lyase; D003545:Cysteine