Epithelial proliferation and expression of the aromatic amine activation enzyme N-acetyltransferase in the prostate of postnatal rat.
Male ACI/seg rats of different postnatal ages were injected, i.p., with 150 mg/kg bromodeoxyuridine one hour before being sacrificed. The incorporation of this compound into the DNA of prostate epithelium was detected immunohistochemically. The labeling indices in prostate epithelium were 16.8, 26.5 and 13.7%, in 3, 11 and 17 days old rats, respectively. It decreased gradually thereafter to 1.8% in 60 days old rats. The mRNA of N-acetyltransferase 1 was detected by a non-isotopic in situ hybridization method using a mixture of specific digoxigenin-11-dUTP-labeled oligodeoxynucleotide probes. The mRNA of N-acetyltransferase 1 was detected during the entire postnatal period. It was not detected in the stromal or endothelial cells. The coincidence of N-acetyltransferase expression in actively proliferating prostate epithelial cells during the postnatal period suggest that the prostate tissue may be particularly sensitive during this period to the carcinogenesis by aromatic and heterocyclic amines.
Archer
C
C
Department of Urology, State University of New York Health Science Center, Syracuse 13210, USA.
Debiec-Rychter
M
M
Morse
P
P
Haas
G P
GP
Wang
C Y
CY
D000367:Age Factors; D000588:Amines / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D000964:Antimetabolites, Antineoplastic / Q000378:metabolism; D001191:Arylamine N-Acetyltransferase / Q000096:biosynthesis*; D002455:Cell Division / Q000187:drug effects; D004848:Epithelium / Q000201:enzymology; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D011467:Prostate / Q000033:anatomy & histology / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011912:Rats, Inbred ACI
D000588:Amines; D000964:Antimetabolites, Antineoplastic; D007527:Isoenzymes; D012333:RNA, Messenger; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1