[Antioxidant effect of 20-hydroxyecdysone in a model system].
Changes in the level of lipid free-radical oxidation in mitochondrial fraction at the presence of 20-hydroxyecdysone in 1, 2, 4, 6, 8 microM concentration with the help of a chemiluminescence (ChL) method were investigated in vitro. Statistically authentic reduction of ChL kinetic parameters--I(s) and tg alpha, at 20-hydroxyecdysone presence in concentration of 2 microM was found. 20-hydroxyecdysone administration in concentration 4 microM and more leads to the statistically authentic change of all four ChL parameters. The antioxidizing effect of 20-hydroxyecdysone was compared with action of such antioxidant as a hydroquinone. The higher activity of 20-hydroxyecdysone as an antioxidant in comparison with the hydroquinone was shown. For changes of all four measured kinetic parameters of ChL, concentration of the hydroquinone as much 2-fold than for 20-hydroxyecdysone is necessary. On the basis of our researches in the model system a conclusion was made that 20-hydroxyecdysone has an antioxidizing action on lipid free radical oxidation in mitochondrial fraction in dependents on concentration. 20-Hydroxyecdysone has antioxidizing properties directly, in these conditions in vitro, when its metabolites formation does not occur yet.
Kuz'menko
A I
AI
A.V. Palladin Institute of Biochemistry of National Academy of Sci, Kyiv.
Morozova
R P
RP
Nikolenko
I A
IA
Donchenko
G V
GV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004441:Ecdysterone / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D004441:Ecdysterone