Improvement of pulmonary preservation with Celsior and Perfadex: impact of storage time on early post-ischemic lung function.
BACKGROUND
Optimal preservation of post-ischemic organ function is a continuing challenge in clinical lung transplantation.


METHODS
Using an established extracorporeal rat lung screening model, the results after preservation of 8 lungs, each with the extracellular-type preservation solutions Celsior and Perfadex using ischemic periods of 2 and 4 hours were compared to the results obtained after 2 hours of preservation with low-potassium Euro-Collins with prostacyclin.


RESULTS
Oxygenation capacity of all Celsior-preserved organs was significantly higher as compared to LPEC lungs (p < 0.01), and after 4 hours of ischemia, lung preservation in terms of post-ischemic oxygenation ability was significantly higher in the Celsior group compared with Perfadex-protected organs (p < 0.01).


CONCLUSIONS
Especially at extended ischemic times Celsior can provide significantly better pulmonary preservation in terms of oxygenation capacity compared to Perfadex solution, which is associated with a post-ischemic lung function only comparable to preservation with modified Euro-Collins solution.
Wittwer
T
T
Department of Cardiothoracic and Vascular Surgery, Hannover Medical School, Germany. Th.Wittwer-MD@t-online.de
Wahlers
T
T
Fehrenbach
A
A
Elki
S
S
Haverich
A
A
D000818:Animals; D001794:Blood Pressure; D002951:Citrates / Q000494:pharmacology*; D004187:Disaccharides / Q000494:pharmacology; D004573:Electrolytes / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D006982:Hypertonic Solutions / Q000494:pharmacology; D008168:Lung / Q000502:physiology*; D016040:Lung Transplantation; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D011651:Pulmonary Artery / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism; D012996:Solutions; D013997:Time Factors
C088453:Celsior; D002951:Citrates; D004187:Disaccharides; D004573:Electrolytes; C037096:Euro-Collins' solution; D005971:Glutamates; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions; C070005:Perfadex; D012996:Solutions; D008353:Mannitol; D006639:Histidine; D005978:Glutathione; D010100:Oxygen