title: Disruption of clh-1, a chloride channel gene, results in a wider body of Caenorhabditis elegans.
abstract: We cloned the clh-1 gene coding for a putative ClC chloride channel in Caenorhabditis elegans. The gene product exhibited a high degree of homology with human ClC-1 and ClC-2. The clh-1 gene was predominantly expressed in the hypodermis, including seam cells. Null mutations of clh-1 caused a significantly wider body and an abnormal alae structure. High osmolarity in the culture medium restored the normal body width of the clh-1 mutants. These results suggest that the clh-1 gene contributes to maintenance of the body width through regulation of osmolarity.
authors.affiliation: Division of Biological Sciences Institute of Scientific and Industrial Research, Osaka University, CREST of the Japan Science and Technology Corporation, Osaka, 567-0047, Japan.
authors.forename: M I
authors.initials: MI
authors.lastname: Petalcorin
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: M
authors.initials: M
authors.lastname: Koga
authors.forename: K
authors.initials: K
authors.lastname: Ogura
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
authors.forename: Y
authors.initials: Y
authors.lastname: Ohshima
authors.forename: M
authors.initials: M
authors.lastname: Futai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001824:Body Constitution / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000648:ultrastructure; D029742:Caenorhabditis elegans Proteins; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009994:Osmolar Concentration; D016415:Sequence Alignment; D012867:Skin / Q000166:cytology
chemical_list: C086321:CLC-1 channel; D029742:Caenorhabditis elegans Proteins; D018118:Chloride Channels; C402825:clh-1 protein, C elegans