title: Evaluation of neutrophil structure and function by electron microscopy: cytochemical studies.
abstract: Methods for studying human neutrophils at the ultrastructural level by enzyme cytochemistry and immunocytochemistry are presented. The focus of these methods is on the analysis of the alkaline phosphatase-positive secretory organelle of these cells. These methods provide unique information which, when coupled with biochemical studies, provide for the most complete analysis of neutrophil structure and function.
authors.affiliation: Department of Physiology and Cell Biology, Ohio State University, 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210, USA. robinson.21@osu.edu
authors.forename: J M
authors.initials: JM
authors.lastname: Robinson
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Seguchi
authors.forename: T
authors.initials: T
authors.lastname: Takizawa
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007399:Interphase / Q000276:immunology; D008854:Microscopy, Electron / Q000379:methods*; D016253:Microscopy, Immunoelectron; D018375:Neutrophil Activation / Q000276:immunology; D009504:Neutrophils / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D010100:Oxygen / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D000469:Alkaline Phosphatase; D010100:Oxygen