title: Nerve growth factor stimulates MAPK via the low affinity receptor p75(LNTR).
abstract: Apart from its high affinity receptor TrkA, nerve growth factor (NGF) can also stimulate the low affinity receptor p75(LNTR) and induce a Trk-independent signaling cascade. We examined the possible involvement of mitogen-activated protein kinase (MAPK) in this signaling pathway in neuronal cultures of the cerebellum of P2-aged rats and PCNA cells; both cell types express p75(LNTR) but not TrkA. We found a fast and transient phosphorylation of p42- and p44-MAPK after stimulation with NGF or C(2)-ceramide which proved to be sensitive to inhibition of MAPK kinase and protein kinase A (PKA). As stimulation with NGF also activated p21Ras it can be concluded that at least part of the observed MAPK activation was effected via p21Ras and via PKA.
authors.affiliation: Molekulare Neurobiochemie, Fakultät Chemie, Ruhr-Universität Bochum, 44780, Bochum, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Susen
authors.forename: R
authors.initials: R
authors.lastname: Heumann
authors.forename: A
authors.initials: A
authors.lastname: Blöchl
mesh_terms: D000367:Age Factors; D000818:Animals; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D015689:Oncogene Protein p21(ras) / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D020800:Receptor, Nerve Growth Factor / Q000187:drug effects* / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; C064769:N-acetylsphingosine; D020800:Receptor, Nerve Growth Factor; D020932:Nerve Growth Factor; D017868:Cyclic AMP-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D015689:Oncogene Protein p21(ras); D013110:Sphingosine