Large-scale monitoring of host cell gene expression during HIV-1 infection using cDNA microarrays.
Human immunodeficiency virus type 1 (HIV-1) infection alters the expression of host cell genes at both the mRNA and protein levels. To obtain a more comprehensive view of the global effects of HIV infection of CD4-positive T-cells at the mRNA level, we performed cDNA microarray analysis on approximately 1500 cellular cDNAs at 2 and 3 days postinfection (p.i.) with HIV-1. Host cell gene expression changed little at 2 days p.i., but at 3 days p.i. 20 cellular genes were identified as differentially expressed. Genes involved in T-cell signaling, subcellular trafficking, and transcriptional regulation, as well as several uncharacterized genes, were among those whose mRNAs were differentially regulated. These results support the hypothesis that HIV-1 infection alters expression of a broad array of cellular genes and provides a framework for future functional studies on the differentially expressed mRNA products.
Geiss
G K
GK
Department of Microbiology, School of Medicine, Seattle, Washington, 98195, USA.
Bumgarner
R E
RE
An
M C
MC
Agy
M B
MB
van 't Wout
A B
AB
Hammersmark
E
E
Carter
V S
VS
Upchurch
D
D
Mullins
J I
JI
Katze
M G
MG
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D002460:Cell Line; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012333:RNA, Messenger