Homozygous deletions define a region of 8p23.2 containing a putative tumor suppressor gene.
Loss of heterozygosity at microsatellite loci in chromosomal band 8p23.2 is a frequent event in squamous cell carcinomas of the head and neck, suggesting that this region contains a putative tumor suppressor. Allelic loss studies on laryngeal and oral/oropharyngeal tumors have restricted the size of this region to approximately 1 cM. A similar pattern of deletions is also observed in prostatic and ovarian adenocarcinomas. As part of an effort to identify this gene by positional cloning, we developed a physical contig consisting of 12 overlapping bacterial artificial chromosome (BAC) clones spanning this interval. We developed sequence-tagged sites from the ends of these BACs and used them, along with seven microsatellite loci, to detect and map homozygous deletions in four head and neck squamous cancer cell lines. Our mapping analysis further restricted the consensus minimal region of deletion to a <191-kb interval.
Sun
P C
PC
Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Schmidt
A P
AP
Pashia
M E
ME
Sunwoo
J B
JB
Scholnick
S B
SB
D002876:Chromosomes, Bacterial / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D020451:Contig Mapping; D015723:Gene Library; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping / Q000379:methods; D017384:Sequence Deletion / Q000235:genetics*; D016324:Sequence Tagged Sites; D014178:Translocation, Genetic / Q000235:genetics