title: Characterization of native interaction of hsp110 with hsp25 and hsc70.
abstract: The 110 kDa heat shock protein (HSP) (hsp110) has been shown to be a diverged subgroup of the hsp70 family and is one of the major HSPs in mammalian cells [1,2]. In examining the native interactions of hsp110, we observed that it is found to reside in a large molecular complex. Immunoblot analysis and co-immunoprecipitation studies identified two other HSPs as components of this complex, hsc70 and hsp25. When examined in vitro, purified hsp25, hsp70 and hsp110 were observed to spontaneously form a large complex and to directly interact with one another. When luciferase was added to this in vitro system, it was observed to migrate into this chaperone complex following heat shock. Examination of two deletion mutants of hsp110 demonstrated that its peptide-binding domain is required for interaction with hsp25, but not with hsc70. The potential function of the hsp110-hsc70-hsp25 complex is discussed.
authors.affiliation: Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: H J
authors.initials: HJ
authors.lastname: Oh
authors.forename: E
authors.initials: E
authors.lastname: Repasky
authors.forename: L
authors.initials: L
authors.lastname: Kazim
authors.forename: J
authors.initials: J
authors.lastname: Subjeck
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D017931:DNA Primers; D050883:HSC70 Heat-Shock Proteins; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D008322:Mammals; D009363:Neoplasm Proteins / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D014407:Tumor Cells, Cultured
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D050883:HSC70 Heat-Shock Proteins; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D009363:Neoplasm Proteins; D008156:Luciferases