title: Ethidium bromide staining reveals rapid cell dispersion in the rat dentate gyrus following ouabain-induced injury.
abstract: The dentate gyrus of the hippocampal formation undergoes extensive network remodelling including progenitor cell proliferation, migration as well as apoptotic cell death in response to prolonged excitotoxic insults. Previous studies have shown that such a proliferative cell population may undergo aberrant migration and later persist in ectopically located positions within the molecular cell layer. In this study we have developed an experimental model to characterize the spatiotemporal patterns of such an injury-induced network remodelling. Ouabain (1 microl, 1 mM), a Na+, K+-ATPase blocker, was stereotactically co-injected into the rat dentate gyrus with ethidium bromide (1 microl, 40 microM). The latter is a fluorescent nucleic acid intercalating dye, which was used for labeling cells undergoing early phases of apoptosis or proliferation. Our results revealed that within an hour after the injection, a subpopulation of cells characterized by spindle- or ovoid-shaped somata and bipolar morphology, were intensely labeled with ethidium bromide. These cells were found initially clustered both inside and outside the dentate granule cell layer and later on markedly increased in number as well as spread radially in the next few hours into the dentate molecular layer. The unusual pattern of cell dispersion encountered in our study may represent aberrantly migrating progenitor cells consistent with earlier observations of ectopically-located granule cells in human temporal lobe epilepsy specimens and epilepsy animal models. Alternatively, the described phenomenon may represent dispersion of Cajal-Retzius cells that may be involved in post-lesion network remodelling.
authors.affiliation: Loeb Health Research Institute, Ottawa Hospital-Civic Campus, University of Ottawa, Ontario, Canada.
authors.forename: A I
authors.initials: AI
authors.lastname: Omar
authors.forename: V V
authors.initials: VV
authors.lastname: Senatorov
authors.forename: B
authors.initials: B
authors.lastname: Hu
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D018891:Dentate Gyrus / Q000187:drug effects* / Q000473:pathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004996:Ethidium; D005456:Fluorescent Dyes; D008297:Male; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats; D020318:Rats, Long-Evans; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D010042:Ouabain; D004996:Ethidium