title: Isolation and characterization of vancomycin-tolerant Streptococcus pneumoniae from the cerebrospinal fluid of a patient who developed recrudescent meningitis.
abstract: The emergence of tolerance to vancomycin has recently been reported in Streptococcus pneumoniae, the most common cause of bacterial meningitis. A vancomycin- and cephalosporin-tolerant strain of S. pneumoniae, the Tupelo strain, was isolated from the cerebrospinal fluid of a patient who then developed recrudescence of meningitis despite treatment with vancomycin and a third-generation cephalosporin. The Tupelo strain evidenced no lysis in the exponential or stationary phase of growth when exposed to vancomycin and only minimal loss of viability. Further characterization revealed normal autolysin expression, localization, and triggering by detergents, indicating that the defect leading to tolerance in the Tupelo strain is in the control pathway for triggering of autolysis. Because tolerance is a precursor phenotype to resistance and may lead to clinical failure of antibiotic therapy, these observations may have important implications for vancomycin use in infections caused by S. pneumoniae.
authors.affiliation: Department of Infectious Diseases, St. Jude Children's Research Hospital; Division of Infectious Diseases, Center, Memphis, Tennessee, USA. jon.mccullers@stjude.org
authors.forename: J A
authors.initials: JA
authors.lastname: McCullers
authors.forename: B K
authors.initials: BK
authors.lastname: English
authors.forename: R
authors.initials: R
authors.lastname: Novak
mesh_terms: D001433:Bacteriolysis; D002511:Cephalosporins / Q000627:therapeutic use; D005260:Female; D006257:Head / Q000473:pathology; D006801:Humans; D007223:Infant; D008279:Magnetic Resonance Imaging; D008586:Meningitis, Pneumococcal / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000188:drug therapy*; D012008:Recurrence; D013131:Spine / Q000473:pathology; D014054:Tomography; D014640:Vancomycin / Q000627:therapeutic use*; D020713:Vancomycin Resistance
chemical_list: D002511:Cephalosporins; D014640:Vancomycin