title: Mucosal mast cell responses are not required for protection against infection with the murine nematode parasite Trichuris muris.
abstract: The involvement of mucosal mast cells (MMC) in protection against infection with the murine nematode parasite Trichuris muris was studied in genetically mast cell-deficient WBB6F1-W/Wv mice and their normal littermates WBB6F1-+/+ mice. Expulsion of T. muris worms occurred in infected +/+ mice, whereas no worm expulsion was observed in infected W/Wv mice where the infection persisted until at least day 46 postinfection. No MMC responses were induced in either infected W/Wv or +/+ mice. Specific IgG1and IgG2a antibodies to T. muris excretory/secretory antigens were observed in infected W/Wv and +/+ mice, and antibody production showed similar kinetics. Interleukin 4 production by concanavalin A (Con A)-stimulated mesenteric lymph node cells (MLNC) was induced preferentially in infected +/+ mice. T. muris infection increased the levels of IFN-gamma produced by Con A-stimulated MLNC of infected W/Wv and +/+ mice, with the levels of IFN-gamma in infected W/Wv mice being higher than those in infected +/+ mice. Taken together, these results indicate that W/Wv and +/+ mice are susceptible and resistant to T. muris infection, respectively, and that MMC responses are not required for protective immunity.
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1, Kitasato, Sagamihara, Kanagawa 228-8555, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Koyama
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D015704:CD4 Antigens / Q000302:isolation & purification; D016827:CD8 Antigens / Q000302:isolation & purification; D002432:Cecum / Q000166:cytology / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000302:isolation & purification; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D008643:Mesentery / Q000166:cytology / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D011947:Receptors, Antigen, B-Cell / Q000302:isolation & purification; D014257:Trichuriasis / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015704:CD4 Antigens; D016827:CD8 Antigens; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell; D015847:Interleukin-4; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens