title: An infective aetiology for vulval lichen sclerosus re-addressed.
abstract: Although there is evidence to support an autoimmune basis for lichen sclerosus, there have also been some studies which suggest an infective aetiology. These include reports of the presence of spirochaetal forms with Steiner silver stains and purplish coccoid forms with Fite stains. We have repeated these studies on vulval biopsies obtained from 16 patients with vulval lichen sclerosus. Using the Steiner silver method we found no evidence of spirochaetal forms in any of the specimens. With the Fite stain we observed purple-staining coccoid forms within the dermis of 13 of the 16 lichen sclerosus specimens. However, these coccoid forms also stained strongly positive with toluidine blue, suggesting they were mast cell granules rather than micro-organisms. We were therefore unable to demonstrate evidence for an infective aetiology in vulval lichen sclerosus, although this cannot yet be excluded. Further work is also needed to understand the significance of mast cells in lichen sclerosus.
authors.affiliation: Department of Dermatology, Oxford Radcliffe Hospitals, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Farrell
authors.forename: P R
authors.initials: PR
authors.lastname: Millard
authors.forename: K H
authors.initials: KH
authors.lastname: Schomberg
authors.forename: F
authors.initials: F
authors.lastname: Wojnarowska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D018459:Lichen Sclerosus et Atrophicus / Q000382:microbiology* / Q000473:pathology; D008875:Middle Aged; D014845:Vulvar Diseases / Q000382:microbiology* / Q000473:pathology