Anti-IL-12 antibody prevents the development and progression of multiple sclerosis-like relapsing--remitting demyelinating disease in NOD mice induced with myelin oligodendrocyte glycoprotein peptide.
Treatment with monoclonal anti-IL-12 antibody injected on day 0, 7 and 10 after immunization with myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 in NOD mice resulted in significant suppression of the development and the severity of the chronic relapsing-remitting experimental autoimmune encephalomyelitis (EAE) both clinically and histologically. The spleen cells from anti-IL-12 antibody treated mice displayed markedly inhibited MOG35-55 specific proliferation and IFN-gamma production. MOG35-55 specific antibody production was enhanced by anti-IL-12 antibody treatment. These results suggest that IL-12 is critically involved in the pathogenesis of MOG-induced EAE and that antibody to IL-12 could be an effective therapeutic agent in the clinical treatment of autoimmune demyelinating diseases such as multiple sclerosis (MS).
Ichikawa
M
M
Department of Pediatrics, Shinshu University School of Medicine, Asahi, Matsumoto, Japan.
Koh
C S
CS
Inoue
A
A
Tsuyusaki
J
J
Yamazaki
M
M
Inaba
Y
Y
Sekiguchi
Y
Y
Itoh
M
M
Yagita
H
H
Komiyama
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D003711:Demyelinating Diseases / Q000276:immunology / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control*; D018450:Disease Progression; D005260:Female; D007114:Immunization; D018664:Interleukin-12 / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control*; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000276:immunology; D013601:T-Lymphocytes / Q000473:pathology
D000911:Antibodies, Monoclonal; D016207:Cytokines; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D018664:Interleukin-12