Association of EGFR gene fragments with nuclear matrix proteins in high and low metastatic melanoma cell lines.
Two melanoma cell lines with different metastatic potential were used to study the association of EGFR gene fragments with the nuclear matrix and its role in cancer metastasis by polymerase chain reaction. A 940 bp positive amplification by PCR using primers I-II was demonstrated in a high metastatic cell line, WM451. A 110 bp positive amplification was shown using primers III-IV in both high and low metastatic cell lines. This finding demonstrates that EGFR gene fragments are tightly bound to the nuclear matrix and suggests that binding ability of this EGFR gene fragment to nuclear matrix seems to be closely related to metastatic potential in melanoma cell lines WM45 1 and WM35.
Wang
Z H
ZH
Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
Ding
M X
MX
Jin
M L
ML
Cheng-Chew
S B
SB
Yun
J P
JP
Wu
B Q
BQ
Chew
E C
EC
D034961:Antigens, Nuclear; D015153:Blotting, Western; D017931:DNA Primers / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics*; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D034961:Antigens, Nuclear; D017931:DNA Primers; D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D066246:ErbB Receptors