Oligonucleotide-conjugated beads for transdominant genetic experiments.
Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligo-nucleotide-conjugated beads and flow cytometry were used to test a strategy that potentially expands the range of such genetic studies. The experimental approach involved creation of tagged expression libraries, introduction of these libraries into cells, growth of the cultured cells for several generations and recovery on oligonucleotide-conjugated beads of sequences that encode growth-modulatory proteins or peptides. Experiments in Saccharomyces cerevisiae demonstrating the feasibility of the strategy are presented.
Feldhaus
M J
MJ
Arcaris Inc., 615 Arapeen Drive, Salt Lake City, UT 84108, USA. feldhaus@arcaris.com
Lualhati
M
M
Cardon
K
K
Roth
B
B
Kamb
A
A
D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D005799:Genes, Dominant; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity
D009841:Oligonucleotides