Metabolite transport in isolated yeast mitochondria: fumarate/malate and succinate/malate antiports.
In this study, we investigated the metabolite permeability of isolated coupled Saccharomyces cerevisiae mitochondria. The occurrence of a fumarate/malate antiporter activity was shown. The activity differs from that of the dicarboxylate carrier (which catalyses the succinate/malate antiport) in (a) kinetics (Km and Vmax values are about 27 microM and 22 nmol min(-1) mg protein(-1) and 70 microM and 4 nmol min(-1) mg protein(-1), respectively), (b) sensitivity to inhibitors, (c) Ki for the competitive inhibitor phenylsuccinate and (d) pH profiles.
Pallotta
M L
ML
Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, Università del Molise, Campobasso, Italy.
Fratianni
A
A
Passarella
S
S
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D017920:Antiporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001693:Biological Transport, Active; D005650:Fumarates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008293:Malates / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure; D013386:Succinates / Q000378:metabolism*
D017920:Antiporters; D005650:Fumarates; D008293:Malates; D013386:Succinates; D000244:Adenosine Diphosphate; C017625:2-phenylsuccinate; C030298:malic acid; C032005:fumaric acid; D000255:Adenosine Triphosphate