[Search for Ki-ras codon 61 mutations in lung adenomas induced in neonatal BALB/c mice with 5-bromodesoxyuridine injection followed by urethane treatment].
BALD/c mice were injected, s.c., 1 mg 5-bromodeoxyuridine (BDU) on days 1.3 and 7 after birth and/or 0.1 ml 5% solution of urethane, 5 times a day every fourth day, i.p., at the age of 3 months. Lung tumors, mostly adenomas, developed in 67% of males and 56% of females, treated with urethane alone. Tumor frequency in response to BDU + urethane rose to 91% in males and 56% in females; multiple neoplasia increased too. In BDU-treated animals, lung tumors appeared in 13% of males and 25% of females whereas in intact controls-3 and 11%, respectively. DNA isolated from paraffin mounts of tumor tissue was used to identify mutations in codon 61 of Ki-ras oncogene. Polymorphism studies of restriction fragment lengths in PCR products failed to detect CAA CTA and CAA CGA sequence changes in 7 samples of DNA. Our findings do not rule out other mutations of RAS oncogenes in our material. They also suggest that further research focus on Ki-ras codon 12 as well as Ha-ras "hot" triplets.
Imianitov
E N
EN
N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.
Togo
A V
AV
Anisimov
V N
VN
D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine; D003062:Codon / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014520:Urethane
D003062:Codon; D004273:DNA, Neoplasm; D014520:Urethane; D001973:Bromodeoxyuridine