title: Synthesis and pharmacological activity of 4-amino-5-chloro-2-methoxy-N-[(2S,4S)-1-ethyl-2-hydroxymethyl-4- pyrrolidinyl]benzamide (TKS159) and its optical isomers.
abstract: Of 4-amino-5-chloro-2-methoxy-N-(1-ethyl-2-hydroxymethyl-4- pyrrolidinyl)benzamide, four optical isomers, (2S,4S)-1 (TKS159), (2S,4R)-25, (2R,4S)-26 and (2R,4R)-27, were prepared from optically active 4-amino-1-ethyl-2-hydroxymethylpyrrolidine di-p-toluenesulfonate [(2S,4S)-14, (2S,4R)-17, (2R,4S)-20 and (2R,4R)-23, respectively]. The requisites, (2S,4S)-14, (2S,4R)-17, (2R,4S)-20 and (2R,4R)-23, were prepared from a commercially available trans-4-hydroxy-L-proline. The absolute configurations of (2S,4S)-1 (TKS159), (2S,4R)-25, (2R,4S)-26 and (2R,4R)-27 were spectroscopically determined. Of the benzamide derivatives, four optical isomers, (2S,4S)-1, (2S,4R)-25, (2R,4S)-26 and (2R,4R)-27, showed a relatively potent affinity for 5-hydroxytryptamine 4 (5-HT4) receptors in a radioligand binding assay ([3H]GR113808). The activities of 25-27 were less effective than that of 1 for the gastric emptying of a phenol red semisolid meal in rats. All this suggests that the most potent of the isomers was 4-amino-5-chloro-2-methoxy-N-[(2S,4S)-1-ethyl-2- hydroxymethyl-4-pyrrolidinyl]benzamide (1).
authors.affiliation: Research and Development Department, Teikoku Chemical Industries Co., Ltd., Hyogo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yanagi
authors.forename: A
authors.initials: A
authors.lastname: Kitajima
authors.forename: K
authors.initials: K
authors.lastname: Anzai
authors.forename: K
authors.initials: K
authors.lastname: Kodama
authors.forename: J
authors.initials: J
authors.lastname: Mizoguchi
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
authors.forename: H
authors.initials: H
authors.lastname: Sakiyama
authors.forename: O
authors.initials: O
authors.lastname: Kamoda
authors.forename: C
authors.initials: C
authors.lastname: Kamei
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D011759:Pyrrolidines / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology*; D011869:Radioligand Assay; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism; D044422:Receptors, Serotonin, 5-HT4; D013057:Spectrum Analysis; D013237:Stereoisomerism
chemical_list: D011759:Pyrrolidines; D011985:Receptors, Serotonin; C098494:TKS 159; D044422:Receptors, Serotonin, 5-HT4