Hemolytic and elastolytic activities influenced by iron in Plesiomonas shigelloides.
The aim of this study was to determine the presence of hemolytic and elastolytic enzymes in several strains of Plesiomonas shigelloides in relation to the availability of iron in culture media. Hemolytic activity and elastolytic activity were detected in strains of P. shigelloides and were enhanced when the strains were grown in an iron-depleted medium and lost after thermal treatment at 100 degrees C for 10 min. Also, elastolytic activity was inactivated by phenylmethyl sulfonyl fluoride, an inhibitor of serine proteases. Hemolytic activity was detected extracellularly in cell-free supernatants, whereas elastin degradation activity was cell associated. Both activities may be related to the virulence of P. shigelloides.
Santos
J A
JA
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de León, Spain. dhtjsb@unileon.es
González
C J
CJ
López
T M
TM
Otero
A
A
García-López
M L
ML
D003470:Culture Media; D006460:Hemolysin Proteins / Q000378:metabolism*; D006358:Hot Temperature; D007501:Iron / Q000494:pharmacology*; D010196:Pancreatic Elastase / Q000378:metabolism*; D016981:Plesiomonas / Q000201:enzymology* / Q000254:growth & development
D003470:Culture Media; D006460:Hemolysin Proteins; D007501:Iron; D010196:Pancreatic Elastase