title: Amelioration of radiation-induced liver damage in partially hepatectomized rats by hepatocyte transplantation.
abstract: Hepatic tumors often recur in the liver after surgical resection. Postoperative radiotherapy (RT) could improve survival, but curative RT may induce delayed life-threatening radiation-induced liver damage. Because RT inhibits liver regeneration, we hypothesized that unirradiated, transplanted hepatocytes would proliferate preferentially in a partially resected and irradiated liver, providing metabolic support. We subjected F344 rats to hepatic RT and partial hepatectomy with/without a single intrasplenic, syngeneic hepatocyte transplantation. Hepatocyte transplantation ameliorated radiation-induced liver damage and improved survival of rats receiving RT after partial hepatectomy. We further demonstrated that transplanted hepatocytes extensively repopulate and function in a heavily irradiated rat liver.
authors.affiliation: Department of Radiation Oncology, Albert Einstein College of Medicine, Bronx, New York 10461, USA. cguhamd@pol.net
authors.forename: C
authors.initials: C
authors.lastname: Guha
authors.forename: A
authors.initials: A
authors.lastname: Sharma
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.forename: A
authors.initials: A
authors.lastname: Alfieri
authors.forename: G R
authors.initials: GR
authors.lastname: Gorla
authors.forename: S
authors.initials: S
authors.lastname: Gagandeep
authors.forename: R
authors.initials: R
authors.lastname: Sokhi
authors.forename: N
authors.initials: N
authors.lastname: Roy-Chowdhury
authors.forename: K E
authors.initials: KE
authors.lastname: Tanaka
authors.forename: B
authors.initials: B
authors.lastname: Vikram
authors.forename: J
authors.initials: J
authors.lastname: Roy-Chowdhury
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D006498:Hepatectomy; D008099:Liver / Q000166:cytology* / Q000473:pathology / Q000528:radiation effects*; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen; D014183:Transplantation, Heterologous; D014185:Transplantation, Isogeneic