Sensitivity of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine nucleotides.
The response of bacteriophage RB69 DNA polymerase to N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGTP), related nucleotides and non-nucleoside inhibitors was measured and compared to values obtained for the closely related DNA polymerase from bacteriophage T4. Both enzymes showed similar responses to inhibitors in terms of Ki values and the ability to utilize BuPdGTP as a substrate. These results provide the relevance of using the recent crystal structure of RB69 DNA polymerase for analysis of BuPdGTP/B family DNA polymerase interactions.
Stattel
J M
JM
Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester 01655, USA.
Wright
G E
GE
D017122:Bacteriophage T4 / Q000737:chemistry; D001435:Bacteriophages / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D003848:Deoxyguanine Nucleotides / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry*; D019384:Nucleic Acid Synthesis Inhibitors
D003848:Deoxyguanine Nucleotides; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; C040364:N(2)-(4-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate; D004259:DNA-Directed DNA Polymerase