title: Light and electron microscopic analysis of controlled injury to follicular unit grafts.
abstract: BACKGROUND
  Careful manipulation of hair units is essential for a good yield of transplanted hair.
  
  
  OBJECTIVE
  To analyze the morphology of dissected follicular units submitted to crushing, stretching, bending, and drying.
  
  
  METHODS
  Follicular units were either crushed, bent, stretched with forceps, or left drying on surgical gloves for 3 minutes. The specimens were fixed and prepared for observation with light microscopy or transmission and scanning electron microscopy.
  
  
  RESULTS
  No alterations were detected in follicular units that had been crushed, bent, or stretched. Major damage occurred in samples that were left to dry on gloves.
  
  
  CONCLUSIONS
  Letting the follicular unit dry appears to be the worst mishandling to which the follicular units may be subjected during routine hair transplantation.
authors.affiliation: Department of Histology, Institute of Biomedical Sciences, University of São Paulo, Brazil. marcelo@gandelman.com
authors.forename: M
authors.initials: M
authors.lastname: Gandelman
authors.forename: A L
authors.initials: AL
authors.lastname: Mota
authors.forename: P A
authors.initials: PA
authors.lastname: Abrahamsohn
authors.forename: S F
authors.initials: SF
authors.lastname: De Oliveira
mesh_terms: D000328:Adult; D006197:Hair / Q000637:transplantation*; D018859:Hair Follicle / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged