title: Quantitative analysis of low-resistance junctions between cultured cells and correlation with gap-junctional areas.
abstract: Electrophysiological studies of low-resistance junctions between Novikoff hepatoma cells grown in suspension cultures were carried out and correlated with gap-junctional areas per inferface determined by freeze-fracture. The mean coupling coefficient between isolated cell pairs was 0.773 +/- 0.025 (SEM) in 67G medium and 0.653 +/- 0.028 in M67 medium; the respective means for the central pairs of four-cell chains were 0.714 +/- 0.034 and 0.595 +/- 0.026. Mean estimates of nonjunctional resistances for cell pairs were 3.0 +/- 0.32 x 10(7) ohm (67G) and 2.01 +/- 0.01 x 10(7) ohm (M67), and the respective estimates for specific nonjunctional resistances were 158.6 +/- 8.1 ohm-cm2 (67G) and 133.0 +/- 812 ohm-cm2 (M67). Mean estimates of junctional conductances were 0.409 +/- 0.058 x 10(-6) mho (67G) and 0.211 +/- 0.018 x 10(-6) mho (M67) for pairs and 0.291 +/- 0.063 x 10(-6) mho (67G) and 0.212 +/- 0.04 mho (M67) for four-cell chains. The mean area of gap junction per interface for separate cell populations was 0.187 +/- 0.049 micron 2 and 0.269 +/- 0.054 micron 2 for cells fixed in loose pellets and in suspension, respectively. When compared with the mean junctional conductance, these values gave specific junctional conductance estimates of 1.13 x 10(2) mho/cm2 and 0.78 x 10(2) mho/cm2, respectively. These values are higher than most previous estimates, but are consistent with the hypothesis that gap-junctional particles contain central hydrophilic channels, about 2 nm in diameter, which have cytoplasmic resistivity.
authors.affiliation: Department of Genetics and Cell Biology, University of Minnesota, Minneapolis 55455, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Sheridan
authors.forename: M
authors.initials: M
authors.lastname: Hammer-Wilson
authors.forename: D
authors.initials: D
authors.lastname: Preus
authors.forename: R G
authors.initials: RG
authors.lastname: Johnson
mesh_terms: D000818:Animals; D004553:Electric Conductivity; D005614:Freeze Fracturing; D017629:Gap Junctions / Q000502:physiology* / Q000648:ultrastructure*; D008114:Liver Neoplasms, Experimental; D008854:Microscopy, Electron; D051381:Rats; D014407:Tumor Cells, Cultured