Homophilic NCAM interactions interfere with L1 stimulated neurite outgrowth.
The cell adhesion molecules NCAM and L1 are considered to play key roles in neuronal development and plasticity. L1 has been shown to interact with NCAM, possibly through NCAM binding to oligomannosidic glycans present in L1. We investigated the effect of recombinant immunoglobulin (Ig) modules of NCAM involved in homophilic NCAM binding, on L1 induced neurite outgrowth from PC12-E2 cells and found a complete inhibition of L1 induced neurite outgrowth after addition of Ig-modules 1, 2 and 3 of NCAM, suggesting that the ligation state of NCAM is crucial for normal L1 signaling.
Kristiansen
L V
LV
Protein Laboratory, Institute of Molecular Pathology, University of Copenhagen, Panum Institute, Blegdamsvej 3C, Bld. 6.2, DK-2200, Copenhagen N, Denmark.
Marques
F A
FA
Soroka
V
V
Ronn
L C
LC
Kiselyov
V
V
Pedersen
N
N
Berezin
V
V
Bock
E
E
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D018920:Coculture Techniques; D007136:Immunoglobulins / Q000378:metabolism; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism* / Q000502:physiology; D016501:Neurites / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D007136:Immunoglobulins; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D011994:Recombinant Proteins