Effects of gender and functional overload on plantaris muscle morphology in the dwarf (HsdOla:dw-4) Lewis rat.
To investigate relationships between pituitary function and gender on skeletal muscle growth and hypertrophy, fiber cross sectional area (CSA) and type were assessed in the plantaris muscle of normal and dwarf (Dw) male and female Lewis rats after 6 weeks of functional overload (FO). Serum growth hormone levels were 70-80% less in Dw rats of both genders, and body mass was 62% greater in normal rats when compared to their Dw counterparts. Muscle weight was affected by gender, dwarfism, and FO as well as a significant gender*Dw*FO interaction. FO increased Type I, IIA, and IIX/B fiber CSA 120%, 102%, and 75%, respectively. Only type 1H fibers exhibited a reduction in CSA as a function of gender or dwarfism. Both type IIA and IIX/B fibers were affected by a significant gender*Dw*FO interaction. Our results suggest that the growth of type II fibers is sensitive to gender and pituitary function, while hypertrophy of type II muscle fibers is a function of the interaction between mechanical load, gender, and pituitary function.
Mulroy
S
S
Department of Physical Activity and Educational Services, The Ohio State University, Columbus 43210-1284, USA.
Blough
E R
ER
Mehta
E K
EK
Myhal
M
M
Linderman
J K
JK
D000818:Animals; D004392:Dwarfism / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000502:physiology; D006984:Hypertrophy; D007334:Insulin-Like Growth Factor I / Q000378:metabolism / Q000502:physiology; D008297:Male; D024510:Muscle Development; D018656:Muscle Fibers, Fast-Twitch / Q000473:pathology; D018482:Muscle, Skeletal / Q000254:growth & development / Q000473:pathology* / Q000503:physiopathology*; D009929:Organ Size / Q000502:physiology; D010902:Pituitary Gland / Q000503:physiopathology; D051381:Rats; D011917:Rats, Inbred Lew; D011922:Rats, Mutant Strains; D012727:Sex Characteristics; D016474:Weight-Bearing
D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone