Interaction of central subgerminal ooplasm with the elementary tissues (endophyll, Rauber's sickle and upper layer) of unincubated avian blastoderms in culture.
By placing a central subgerminal ooplasmic mass over isolated parts (alone or in association) of unincubated avian blastoderms and culture, we obtained an improvement in, or in some cases restoration of normal development. The evolution of small rectangular fragments (isolates) excised from different regions of the unincubated blastoderm was observed in association or not with subgerminal ooplasm. The only type of differentiation that was clearly distinguished in these isolates (taken from the caudocentral area centralis region) was a so-called 'primary neurula' formed by the endophyll and an associated thickened upper layer. In the present study, we also demonstrate that after removal of the area centralis from an unincubated caudal blastoderm quadrant, the upper layer (UL) and endophyll can no longer be restored from the associated subgerminal ooplasm (and form a miniature embryo), as is the case after removal of the endophyll alone. A deep layer (containing the endophyll) reformed during the migration of Rauber's sickle-derived cells into the neighbouring central subgerminal ooplasm only in the presence of the upper layer. This suggests that the upper layer has an early influence on the cells containing the original central deep ooplasm (delta ooplasm) to form the endophyll. The present study offers supplementary arguments in favour of the hypothesis that the endophyll is an inductor of preneurulation.
Callebaut
M
M
Laboratory of Human Anatomy and Embryology, RUCA, UA, Antwerp, Belgium. macal@ruca.ua.ac.be
Van Nueten
E
E
Bortier
H
H
Harrisson
F
F
D000818:Animals; D001756:Blastoderm / Q000502:physiology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002642:Chick Embryo; D003370:Coturnix / Q000196:embryology; D046508:Culture Techniques; D003593:Cytoplasm / Q000502:physiology*