title: Immunogenicity studies with a genetically engineered hexavalent PorA and a wild-type meningococcal group B outer membrane vesicle vaccine in infant cynomolgus monkeys.
abstract: The immunogenicity of two meningococcal outer membrane vesicle (OMV) vaccines, namely the Norwegian wild-type OMV vaccine and the Dutch hexavalent PorA OMV vaccine, were examined in infant cynomolgus monkeys. For the first time, a wild-type- and a recombinant OMV vaccine were compared. Furthermore, the induction of memory and the persistence of circulating antibodies were measured. The Norwegian vaccine contained all four classes of major outer membrane proteins (OMP) and wild-type L3/L8 lipopolysaccharide (LPS). The Dutch vaccine consisted for 90% of class 1 OMPs, had low expression of class 4 and 5 OMP, and GalE LPS. Three infant monkeys were immunised with a human dose at the age of 1.5, 2.5 and 4.5 months. Two monkeys of each group received a fourth dose at the age of 11 months. In ELISA, both OMV vaccines were immunogenic and induced booster responses, particularly after the fourth immunisation. The Norwegian vaccine mostly induced sero-subtype P1.7,16 specific serum bactericidal antibodies (SBA), although some other SBA were induced as well. The antibody responses against P1.7,16, induced by the Norwegian vaccine, were generally higher than for the Dutch vaccine. However, the Dutch vaccine induced PorA specific SBA against all six sero-subtypes included in the vaccine showing differences in the magnitude of SBA responses to the various PorAs.
authors.affiliation: Laboratory for Vaccine Research, National Institute of Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, Bilthoven, Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Rouppe van der Voort
authors.forename: M
authors.initials: M
authors.lastname: Schuller
authors.forename: J
authors.initials: J
authors.lastname: Holst
authors.forename: P
authors.initials: P
authors.lastname: de Vries
authors.forename: P
authors.initials: P
authors.lastname: van der Ley
authors.forename: G
authors.initials: G
authors.lastname: van den Dobbelsteen
authors.forename: J
authors.initials: J
authors.lastname: Poolman
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D016667:Bacterial Capsules; D001428:Bacterial Vaccines / Q000276:immunology*; D001790:Blood Physiological Phenomena; D004797:Enzyme-Linked Immunosorbent Assay; D005818:Genetic Engineering; D007109:Immunity; D008252:Macaca fascicularis; D022401:Meningococcal Vaccines; D009345:Neisseria meningitidis; D011135:Polysaccharides, Bacterial / Q000235:genetics / Q000276:immunology*; D018272:Porins / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D022401:Meningococcal Vaccines; D011135:Polysaccharides, Bacterial; D018272:Porins; C039455:capsular polysaccharide, meningococcal group B; C057699:porin protein, Neisseria