Comet assay in genetic ecotoxicology: a review.
The Comet assay, also called the single cell gel electrophoresis (SCGE) assay or microgel electrophoresis (MGE) assay, primarily measures DNA strand breakage in single cells. Since the protocol was published by Singh et al. [1988], its use has increased in different topic areas: clinical applications, human monitoring, radiation biology, genetic toxicology, and genetic ecotoxicology. This study is a review of the investigations that have involved the alkaline version of the Comet assay in genetic ecotoxicology. It focuses mainly on the type of organisms (plants, worms, molluscs, fish, amphibians, and mammalians) but also on the type of cells that have been used for ecotoxicological studies. In the 23 papers published since 1993 and presented here, the original test procedure may have been slightly modified according to the cell type. In vitro and in vivo experiments as well as in situ studies have been carried out in various environments (water, soil, and air). Although the Comet assay is able to detect genotoxic effects of chemical and physical agents, only chemical substances and environmental complex mixtures will be considered in this review.
Cotelle
S
S
Centre des Sciences de l'Environnement, Université de Metz, Metz, France. cotelle@sciences.univ-metz.fr
Férard
J F
JF
D000663:Amphibians / Q000235:genetics; D000818:Animals; D020552:Comet Assay / Q000379:methods*; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes / Q000235:genetics; D006801:Humans; D008322:Mammals / Q000235:genetics; D008974:Mollusca / Q000187:drug effects / Q000235:genetics; D009835:Oligochaeta / Q000187:drug effects / Q000235:genetics; D010944:Plants / Q000187:drug effects / Q000235:genetics