Distribution of 2-chloro-2'-deoxyadenosine, 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine, fludarabine and cytarabine in mice: a whole-body autoradiography study.
The distribution characteristics of tritiated nucleoside analogs, 2-chloro-2'-deoxyadeonosine (CdA), 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine (CAFdA), 2-fluoroarabinosyladenine (F-ara-A) and cytosine arabinoside (ara-C) were compared in mice using whole-body autoradiography. CdA, CAFdA and F-ara-A have quite similar molecular structures, but they differ substantially in clinical activity as well as the side effects. Eight mice were injected intravenously in couples. One mouse from each pair was killed 20 min postinjection and the other mouse from each pair 4 h after the injection. The distribution of the label was then analyzed by whole-body autoradiography. The distribution of the nucleoside analogs was rapid and uniform. High concentrations were found in highly perfused organs. After 4 h the overall concentration had decreased but relatively high activities were found in the skin for CdA and CAFdA, in the thymus for ara-C and the bone marrow for CdA. Both CdA and CAFdA were found in the brain, but the concentration was surprisingly lower after 4 h for CAFdA, a lipophilic and more stable analog as compared to CdA. There was an uptake of CdA, F-ara-A and CAFdA in the skin. There were signs of retention of ara-C in parts of the thymus. The present investigations indicate that the nucleoside analog transport to the brain in mice is not primarily dependent upon passive diffusion over a lipophilic barrier, but suggestive of a specific transport mechanism.
Lindemalm
S
S
Department of Clinical Pharmacology,Karolinska Hospital, Stockholm, Sweden.
Liliemark
J
J
Larsson
B S
BS
Albertioni
F
F
D000227:Adenine Nucleotides; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000493:pharmacokinetics; D001087:Arabinonucleosides / Q000493:pharmacokinetics*; D001345:Autoradiography; D001921:Brain / Q000378:metabolism; D017338:Cladribine / Q000493:pharmacokinetics*; D000077866:Clofarabine; D003561:Cytarabine / Q000493:pharmacokinetics*; D050356:Lipid Metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D013950:Thymus Gland / Q000378:metabolism; D014018:Tissue Distribution; D014316:Tritium; D014740:Vidarabine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D000227:Adenine Nucleotides; D000964:Antimetabolites, Antineoplastic; D001087:Arabinonucleosides; D003561:Cytarabine; D014316:Tritium; D017338:Cladribine; D000077866:Clofarabine; D014740:Vidarabine; C024352:fludarabine