title: Expression of factor X in both the rat brain and cells of the central nervous system.
abstract: Prothrombin is expressed in the central and peripheral nervous systems. However, the mechanism responsible for the activation of prothrombin to thrombin by the activated form of factor X in the central and peripheral nervous systems remains to be explored. Here, we investigated the expression of factor X mRNA in the brain and some cell lines derived from the central nervous system. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated the expression of mRNA encoding factor X in the rat brain, A172 (human glioblastoma) and GOTO (human neuroblastoma) cells. The sequences of PCR-derived fragments were identical to those reported for rat and human factor X. These results indicated the synthesis of factor X in the cells of the central nervous system.
authors.affiliation: Department of Biochemistry, Meiji Pharmaceutical University, Noshio, Kiyose, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shikamoto
authors.forename: T
authors.initials: T
authors.lastname: Morita
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D016543:Central Nervous System Neoplasms; D005170:Factor X / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D005909:Glioblastoma; D006801:Humans; D008099:Liver / Q000378:metabolism; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D005170:Factor X