Isolation of adipophilin and butyrophilin from bovine milk and characterization of a cDNA encoding adipophilin.
The milk fat globule membrane-associated proteins adipophilin (alias adipocyte differentiation-related protein) and butyrophilin were purified from bovine milk by reverse-phase chromatography. The nucleotide sequence of bovine adipophilin was obtained via peptide mapping and sequencing of a mammary gland cDNA clone, which comprises 1841 nucleotides and has an open reading frame of 450 amino acids. By peptide mapping, 19% of the amino acid sequence was confirmed. The obtained amino acid sequence has 87 and 80% identical residues with human and mouse adipophilin, respectively. Alignment with the proteins perilipin and TIP47 revealed two highly conserved segments, which may assemble into amphipathic alpha-helices.
Nielsen
R L
RL
Protein Chemistry Laboratory, University of Aarhus, Denmark.
Andersen
M H
MH
Mabhout
P
P
Berglund
L
L
Petersen
T E
TE
Rasmussen
J T
JT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000071797:Butyrophilins; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000032:analysis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D008565:Membrane Proteins; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification*; D000070780:Perilipin-2; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D000071797:Butyrophilins; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008894:Milk Proteins; C000602982:PLIN2 protein, human; D010455:Peptides; D000070780:Perilipin-2; C000602983:Plin2 protein, mouse