Evaluation of the BACTEC MGIT 960 and the MB/BacT systems for recovery of mycobacteria from clinical specimens and for species identification by DNA AccuProbe.
A total of 120 mycobacterial isolates were recovered from 1,068 clinical specimens. Of these, 82.5% were in MGIT 960, 83.3% were in MB/BacT, 80% were in BACTEC 460, and 70% were on Löwenstein-Jensen medium. Mean times to detection of Mycobacterium tuberculosis (n = 96) were significantly shorter with MGIT 960 (12.6 days, P = 0.003) and BACTEC 460 (11.8 days, P < 0.001) than with MB/BacT (15.9 days). Although, MGIT 960 showed the lowest rate of recovery of M. kansasii genotype I (64.3%), the earliest growth was detected with this system (8.9 days). Low and similar rates of contamination were obtained with MGIT 960 (3.3%) and MB/BacT (3%). The AccuProbe test for identification showed excellent sensitivities with MGIT 960 (96. 8%) and MB/BacT (100%) cultures. In addition to being nonradiometric, both MGIT 960 and MB/BacT are accurate, rapid, and labor-saving detection systems which could replace the radiometric method.
Alcaide
F
F
Servei de Microbiologia, Hospital "Princeps d'Espanya," Ciutat Sanitària i Universitària de Bellvitge, 08907 L'Hospitalet de Llobregat, Barcelona, Spain. falcaide@csub.scs.es
Benítez
M A
MA
Escribà
J M
JM
Martín
R
R
D015373:Bacterial Typing Techniques; D003470:Culture Media; D015342:DNA Probes; D006801:Humans; D009161:Mycobacterium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic
D003470:Culture Media; D015342:DNA Probes; D011933:Reagent Kits, Diagnostic