Antithrombotic activity of avian crown dermatan sulfate.
The antithrombotic activity of dermatan sulfate from avian crown with the mean molecular weight of 38000 was compared to those from bovine intestine with the mean molecular weight of 16000 in vivo and in vitro. In an in vitro test, bovine intestine dermatan sulfate exhibited stronger effects on stimulation of heparin cofactor II and activation of Glu-plasminogen by tissue plasminogen activator. In vivo, avian crown dermatan sulfate more effectively prevented the development of thrombus in a rat deep vein thrombosis model. The measurement of plasma levels of these two kinds of dermatan sulfate revealed that avian crown dermatan sulfate circulated in higher concentration and longer duration than bovine intestine dermatan sulfate after intravenous administration to rats.
Kyogashima
M
M
Seikagaku Corporation, Tokyo Research Institute, Japan. kyogashimamo@ma4.justnet.ne.jp
Onaya
J
J
Miyauchi
S
S
Arai
M
M
Shibata
Y
Y
Suda
A
A
Sakai
T
T
Takada
Y
Y
Takada
A
A
D000818:Animals; D000925:Anticoagulants / Q000097:blood / Q000737:chemistry* / Q000627:therapeutic use*; D002417:Cattle; D002645:Chickens; D019765:Chondroitin ABC Lyase / Q000378:metabolism; D002850:Chromatography, Gel; D003871:Dermatan Sulfate / Q000097:blood / Q000737:chemistry* / Q000627:therapeutic use*; D004187:Disaccharides / Q000032:analysis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004789:Enzyme Activation / Q000187:drug effects; D006207:Half-Life; D015844:Heparin Cofactor II / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D010958:Plasminogen / Q000378:metabolism; D051381:Rats; D013045:Species Specificity; D013429:Sulfatases / Q000378:metabolism; D013917:Thrombin / Q000037:antagonists & inhibitors; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D020246:Venous Thrombosis / Q000188:drug therapy* / Q000517:prevention & control
D000925:Anticoagulants; D004187:Disaccharides; D003871:Dermatan Sulfate; D015844:Heparin Cofactor II; D010958:Plasminogen; D013429:Sulfatases; C046341:chondro-6-sulfatase; D013917:Thrombin; D010959:Tissue Plasminogen Activator; D019765:Chondroitin ABC Lyase