Polymerase chain reaction assay to identify all immature stages of two species of the Anopheles quadrimaculatus sibling species complex (Diptera: Culicidae).
A polymerase chain reaction assay that detects differences in the 2nd internal transcribed spacer of ribosomal DNA was tested as a means to identify all immature stages of 2 species in the Anopheles quadrimaculatus sibling species complex. The technique was successful in amplifying DNA from eggs, 1st-4th instars, and pupae of An. quadrilmaculatus Say and An. inundatus Reinert.
Rutledge
C R
CR
Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge 70893, USA.
Wesson
D M
DM
Meek
C L
CL
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis*; D005260:Female; D007303:Insect Vectors; D007814:Larva / Q000235:genetics; D010063:Ovum; D016133:Polymerase Chain Reaction / Q000379:methods*
D004275:DNA, Ribosomal