Rapid diagnosis of primary dengue fever by the immunochromatographic test and by electron microscopy--a case report.
A 21-year-old woman presented with an acute febrile illness after a two-week holiday in Jamaica. Her symptoms started two days after return, with sudden onset of continuous high fever (> 39 degrees C), dizziness and nausea. Three days later she developed a generalized macular rash, which led to the tentative diagnosis "acute dengue fever." Laboratory confirmation was achieved by demonstrating anti-dengue IgM and IgG antibodies in paired sera; in addition, flavivirus particles were directly visualized by electron microscopy.
Allwinn
R
R
Institute for Medical Virology, J.W. Goethe University Frankfurt/Main, Germany. Allwinn@em.uni-frankfurt.de
Schieferstein
C
C
Glauke
S
S
Doerr
H W
HW
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D002845:Chromatography; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008854:Microscopy, Electron
D000914:Antibodies, Viral