title: Isolation and characterization of a calendic acid producing (8,11)-linoleoyl desaturase.
abstract: For the biosynthesis of calendic acid a (8,11)-linoleoyl desaturase activity has been proposed. To isolate this desaturase, PCR-based cloning was used. The open reading frame of the isolated full-length cDNA is a 1131 bp sequence encoding a protein of 377 amino acids. For functional identification the cDNA was expressed in Saccharomyces cerevisiae, and formation of calendic acid was analyzed by RP-HPLC. The expression of the heterologous enzyme resulted in a significant amount of calendic acid presumably esterified within phospholipids. The results presented here identify a gene encoding a new type of (1,4)-acyl lipid desaturase.
authors.affiliation: Leibniz Institut für Pflanzenbiochemie, Weinberg, Halle, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Fritsche
authors.forename: E
authors.initials: E
authors.lastname: Hornung
authors.forename: N
authors.initials: N
authors.lastname: Peitzsch
authors.forename: A
authors.initials: A
authors.lastname: Renz
authors.forename: I
authors.initials: I
authors.lastname: Feussner
mesh_terms: D000595:Amino Acid Sequence; D020838:Calendula / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D005227:Fatty Acids / Q000032:analysis*; D050602:Linoleoyl-CoA Desaturase; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D005227:Fatty Acids; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase