Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355.
The gene encoding alternansucrase (ASR) from Leuconostoc mesenteroides NRRL B-1355, an original sucrose glucosyltransferase (GTF) specific to alternating alpha-1,3 and alpha-1,6 glucosidic bond synthesis, was cloned, sequenced and expressed into Escherichia coli. Recombinant enzyme catalyzed oligoalternan synthesis from sucrose and maltose acceptor. From sequence comparison, it appears that ASR possesses the same domains as those described for GTFs specific to either contiguous alpha-1,3 osidic bond or contiguous alpha-1,6 osidic bond synthesis. However, the variable region and the glucan binding domain are longer than in other GTFs (by 100 and 200 amino acids respectively). The N-catalytic domain which presents 49% identity with the other GTFs from L. mesenteroides possesses the three determinants potentially involved in the glucosyl enzyme formation.
Argüello-Morales
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Centre de Bioingénierie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, INSA, 135 Avenue de Rangueil, 310077, Toulouse, France.
Remaud-Simeon
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Pizzut
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Sarçabal
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Willemot
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Monsan
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D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D016695:Glycosyltransferases; D007934:Leuconostoc / Q000201:enzymology / Q000235:genetics*; D008320:Maltose / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013395:Sucrose / Q000378:metabolism
D009844:Oligosaccharides; D013395:Sucrose; D008320:Maltose; D016695:Glycosyltransferases; C033837:alternansucrase; D006026:Glycoside Hydrolases