PRK and LASIK--their potential risk of cataractogenesis: lipid peroxidation changes in the aqueous humor and crystalline lens of rabbits.
OBJECTIVE
There are insufficient data on the possible cataractogenic side effects of excimer laser corneal surgery. Higher malondialdehyde (MDA) levels could indicate oxidative events related to the cataractogenic process. We therefore examined MDA levels after refractive laser surgery.


METHODS
Six white Russian rabbits received laser in situ keratomileusis (LASIK) (Schwind keratome) in the right eye and a 250-microm-deep microkeratome cut (Schwind microkeratome) in the left eye. Six others underwent photorefractive keratectomy (PRK) in the right eye; the left eye remained untreated. The 180 mJ/cm2 fluence applied at a rate of 10 Hz with an optical zone diameter of 5 mm in all rabbits (438 pulses) resulted in an estimated central photoablation depth of 116 microm. Two weeks later, lenses and aqueous were taken immediately after death. MDA was detected in aqueous and homogenate of lenses after reacting with thiobarbituric acid (TBA). MDA bound to TBA (MDA-TBA) was specifically analyzed by high-performance liquid chromatography (HPLC) (excitation, 525 nm; emission, 551 nm) using phosphate-buffered methanol as eluent.


RESULTS
No significant laser-induced MDA alteration was found in either the aqueous or the lens. The microkeratome group, however, had two to three times higher MDA levels in the lenses than the control group (p = 0.12) or the PRK (p = 0.03) group.


CONCLUSIONS
Elevation of MDA in the lens of the microkeratome group indicates that LASIK, but not PRK, may be a risk factor in cataractogenesis. The increased MDA levels in the LASIK group are probably caused by the microkeratome incision rather than the secondary radiation of the excimer laser. Postoperative inflammation may explain the surprising results.
Wachtlin
J
J
Department of Ophthalmology, Free University of Berlin, Germany.
Blasig
I E
IE
Schründer
S
S
Langenbeck
K
K
Hoffmann
F
F
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D015415:Biomarkers; D002386:Cataract / Q000209:etiology* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D020731:Keratomileusis, Laser In Situ / Q000009:adverse effects*; D054018:Lasers, Excimer; D007908:Lens, Crystalline / Q000378:metabolism*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D018903:Photorefractive Keratectomy / Q000009:adverse effects*; D011817:Rabbits; D012307:Risk Factors
D015415:Biomarkers; D008315:Malondialdehyde