Molecular grouping of Listeria monocytogenes based on the sequence of the inIB gene.
The major part of the gene inlB was sequenced in 24 strains of Listeria monocytogenes belonging to serovars 1/2a, 1/2b, 1/2c, 3b and 4b. A phylogenetic analysis based on the inlB nucleotide sequences showed that strains of serovars 1/2a and 1/2c were closely related, as well as those of serovars 1/2b and 3b. Strains sharing serovar 4b could be divided into two distinct groups. There were differences in amino-acid sequence between all serovars except between serovars 1/2b and 3b. Differences in amino-acid sequence were also seen within each of the serovars 1/2a and 4b. The data presented indicate that the inlB gene may be useful for typing purposes as an alternative or complement to serotyping.
Ericsson
Henrik
H
Unnerstad
Helle
H
Mattsson
Jens G
JG
Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7009, 750 07 Uppsala and *Department of Parasitology, National Veterinary Institute, PO Box 7073, 750 07 Uppsala, Sweden.
Danielsson-Tham
Marie-Louise
ML
Tham
Wilhelm
W
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping
D001426:Bacterial Proteins; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes