title: Acute follicular conjunctivitis caused by adenovirus type 34.
abstract: OBJECTIVE
  Adenovirus type 34 belongs to adenovirus subgenus B. The prototype virus of adenovirus 34 was isolated from a renal transplant recipient. However, no case of acute conjunctivitis caused by adenovirus 34 has been reported. Recently, we encountered two cases of acute follicular conjunctivitis in which adenovirus 34 was isolated.
  
  
  METHODS
  The clinical isolates were identified by the standard neutralization test. The sequences of seven hypervariable regions in the hexon protein of these cases were compared with those of several prototype strains of adenovirus subgenus B.
  
  
  RESULTS
  The cases were middle-aged, 34 and 41 years old, and male, and they exhibited moderate conjunctivitis with upper respiratory tract symptoms. Isolates from cell culture were identified as adenovirus 34 by NT. The mean homology rate (percentage of total number of coincident amino acids in the total length of amino acids in seven hypervariable regions) between clinical isolates and the adenovirus 34 prototype was 96.5%; in contrast, those between clinical isolates and the prototypes of adenovirus 11, adenovirus 14, and adenovirus 35 were 55.6%, 66.7%, and 57.9%, respectively. The results of conventional serotyping by neutralization test were confirmed by these values.
  
  
  CONCLUSIONS
  These results indicate that adenovirus 34 may induce acute conjunctivitis in immunocompetent subjects and that special attention should be paid to adenovirus 34 as a causative agent for adenoviral conjunctivitis.
authors.affiliation: Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan. euchio@urahp.yokohama-cu.ac.jp
authors.forename: E
authors.initials: E
authors.lastname: Uchio
authors.forename: N
authors.initials: N
authors.lastname: Matsuura
authors.forename: S
authors.initials: S
authors.lastname: Takeuchi
authors.forename: N
authors.initials: N
authors.lastname: Itoh
authors.forename: H
authors.initials: H
authors.lastname: Ishiko
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: S
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000208:Acute Disease; D017456:Adenovirus Early Proteins / Q000235:genetics; D000258:Adenovirus Infections, Human / Q000150:complications* / Q000188:drug therapy / Q000473:pathology / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D003228:Conjunctiva / Q000473:pathology / Q000821:virology; D003236:Conjunctivitis, Viral / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology / Q000821:virology; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction
chemical_list: D017456:Adenovirus Early Proteins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D017931:DNA Primers; D004279:DNA, Viral