Characterization of P8 and J8/7 elements in the conserved core of the tetrahymena group I intron ribozyme.
The universally conserved core region in the group I intron ribozymes is responsible for its catalytic activity. The structural elements in this region have been known to organize the active site of this class of ribozymes. However, it has been unclear whether all elements are requisite or some elements are dispensable for conducting the catalysis. To investigate the necessity of these elements in the catalysis, we prepared and examined a series of mutants having a nick or deletion in these elements. In this report, we show that two elements, P8 and 5' portion of J8/7, are nonessential for activity.
Ikawa
Y
Y
Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan.
Shiraishi
H
H
Inoue
T
T
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D017124:Conserved Sequence; D007438:Introns; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013768:Tetrahymena / Q000235:genetics*
D016337:RNA, Catalytic