Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel mscL.
Upon osmotic downshock, a few cytoplasmic proteins, including thioredoxin, elongation factor Tu (EF-Tu), and DnaK, are released from Tris-EDTA-treated Escherichia coli cells by an unknown mechanism. We have shown previously that deletion of mscL, the gene coding for the mechanosensitive channel of the plasma membrane with the highest conductance, prevents the release of thioredoxin. We confirm and extend the implication of MscL in this process by showing that the release of EF-Tu and DnaK is severely impaired in MscL-deficient strains. Release of these proteins is not observed in the absence of a Tris-EDTA treatment which disrupts the outer membrane, indicating that, in intact cells, they are transferred to the periplasm upon shock, presumably through the MscL channel.
Berrier
C
C
Laboratoire des Biomembranes, UMR CNRS 8619, Université Paris-Sud, 91405 Orsay Cedex, France.
Garrigues
A
A
Richarme
G
G
Ghazi
A
A
D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism* / Q000502:physiology; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism*; D009997:Osmotic Pressure; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D019897:Periplasm / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D007473:Ion Channels; C086596:MscL protein, E coli; D010444:Peptide Elongation Factor Tu; C063842:dnaK protein, E coli