Interaction between sodium ascorbate and polyphenols.
The interaction between sodium ascorbate and polyphenols was investigated by two different parameters: radical intensity and cytotoxicity induction. ESR spectroscopy shows that sodium ascorbate, lignins (alkali-lignin, lignin sulfonate, pine cone-derived lignin) and tannins (gallic acid, epigallocatechin gallate (EGCg), tannic acid) all produced radicals with different intensities under alkaline conditions. All lignin samples significantly enhanced the radical intensity and cytotoxic activity of sodium ascorbate, whereas all tannin samples slightly reduced the radical intensity of sodium ascorbate. Sodium ascorbate efficiently scavenged the radical and cytotoxic activity of gallic acid, as expected by the ionization potential and heat of reaction by the PM3 semiempirical method. EGCg and tannic acid slightly reduced the cytotoxic activity of sodium ascorbate. The present study demonstrates that lignins and tannins modify the radical intensity and cytotoxic activity of sodium ascorbate in different manners, and suggests the applicability of ESR spectroscopy for the screening of drug to drug interaction.
Satoh
K
K
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Ida
Y
Y
Ishihara
M
M
Sakagami
H
H
D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D005419:Flavonoids; D005707:Gallic Acid / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D047348:Hydrolyzable Tannins / Q000378:metabolism; D008956:Models, Chemical; D009062:Mouth Neoplasms / Q000378:metabolism; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D059808:Polyphenols; D013997:Time Factors; D014407:Tumor Cells, Cultured
D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D005707:Gallic Acid; D001205:Ascorbic Acid