title: Post-translational regulation of Adr1 activity is mediated by its DNA binding domain.
abstract: ADR1 encodes a transcriptional activator that regulates genes involved in carbon source utilization in Saccharomyces cerevisiae. ADR1 is itself repressed by glucose, but the significance of this repression for regulating target genes is not known. To test if the reduction in Adr1 levels contributes to glucose repression of ADH2 expression, we generated yeast strains in which the level of Adr1 produced during growth in glucose-containing medium is similar to that present in wild-type cells grown in the absence of glucose. In these Adr1-overproducing strains, ADH2 expression remained tightly repressed, and UAS1, the element in the ADH2 promoter that binds Adr1, was sufficient to maintain glucose repression. Post-translational modification of Adr1 activity is implicated in repression, since ADH2 derepression occurred in the absence of de novo protein synthesis. The N-terminal 172 amino acids of Adr1, containing the DNA binding and nuclear localization domains, fused to the Herpesvirus VP16-encoded transcription activation domain, conferred regulated expression at UAS1. Nuclear localization of an Adr1-GFP fusion protein was not glucose-regulated, suggesting that the DNA binding domain of Adr1 is sufficient to confer regulated expression on target genes. A Gal4-Adr1 fusion protein was unable to confer glucose repression at GAL4-dependent promoters, suggesting that regulation mediated by ADR1 is specific to UAS1.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, Washington 98195-7350, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Sloan
authors.forename: K M
authors.initials: KM
authors.lastname: Dombek
authors.forename: E T
authors.initials: ET
authors.lastname: Young
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D049452:Green Fluorescent Proteins; D007527:Isoenzymes / Q000235:genetics; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D016335:Zinc Fingers; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: C074682:ADR1 protein, S cerevisiae; D017931:DNA Primers; D004268:DNA-Binding Proteins; D007527:Isoenzymes; D008164:Luminescent Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D004247:DNA; D000426:Alcohol Dehydrogenase; D001616:beta-Galactosidase; D005947:Glucose