Changes in mRNA levels of fibrinogen subunit polypeptides in rats defibrinogenated with batroxobin.
Batroxobin is a snake venom that is a thrombinlike enzyme used for clinical treatment. We analyzed hepatic mRNA levels for fibrinogen subunit polypeptides and prothrombin by reverse transcription-polymerase chain reaction as well as coagulation and fibrinolysis factors in plasma 1, 3, 5 and 24 hours after Batroxobin treatment (3 BU/100 g) in rats. The mRNA levels of alpha- and beta-chains of fibrinogen were significantly increased with decreases in plasma fibrinogen, alpha2-plasmin inhibitor, and plasminogen levels, while the mRNA levels for prothrombin remained unchanged. These results suggest that fibrinogen mRNA synthesis is regulated by plasma fibrinogen levels in Batroxobin-induced defibrinogenated rats.
Iwai
S
S
Department of Pharmacology, Showa University School of Medicine, Tokyo, Japan. shinichi@med.showa-u.ac.jp
Okazaki
M
M
Kiuchi
Y
Y
Oguchi
K
K
D000818:Animals; D012103:Batroxobin / Q000494:pharmacology*; D001780:Blood Coagulation Tests; D005340:Fibrinogen / Q000235:genetics* / Q000378:metabolism*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010958:Plasminogen / Q000378:metabolism; D011516:Prothrombin / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D000979:alpha-2-Antiplasmin / Q000378:metabolism
D005343:Fibrinolytic Agents; D012333:RNA, Messenger; D000979:alpha-2-Antiplasmin; D011516:Prothrombin; D005340:Fibrinogen; D010958:Plasminogen; D012103:Batroxobin