title: An assessment of the strength of NG108-15 cell adhesion to chemically modified surfaces.
abstract: The strength of adhesion of NG108-15 cells to glass substrates modified with adsorbed proteins (laminin and poly-ornithine) or modified with covalently bound peptides (tri-ornithine and Tyr-Ile-Gly-Ser-Arg) was quantitatively assessed, by determining the shear stresses necessary to denude the cells from substrates using a spinning disk device. The shear stresses required to detach NG108-15 cells from glass modified with either adsorbed poly-ornithine or with both poly-ornithine and laminin were significantly (P < 0.05) higher than the shear stresses required to detach the cells from plain glass substrates. Covalent surface modifications resulted in higher strengths of NG108-15 adhesion than were exhibited on surfaces modified with adsorbed proteins. NG108-15 cell adhesion strength was maximal on surfaces covalently modified with only amine groups (without any peptides or proteins). These results indicate that general (i.e., not necessarily receptor-specific) surface modification strategies, which increase the net surface charge of a substrate, will elicit strong adhesion of NG108-15 cells.
authors.affiliation: School of Mechanical Engineering, Georgia Institute of Technology, Atlanta 30332-0405, USA. robert.cargill@me.gatech.edu
authors.forename: R S
authors.initials: RS
authors.lastname: Cargill
authors.forename: K C
authors.initials: KC
authors.lastname: Dee
authors.forename: S
authors.initials: S
authors.lastname: Malcolm
mesh_terms: D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology*; D018929:Cell Culture Techniques; D006801:Humans; D007797:Laminin / Q000502:physiology; D008858:Microscopy, Phase-Contrast; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010455:Peptides / Q000502:physiology; D013314:Stress, Mechanical; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D015815:Cell Adhesion Molecules; D007797:Laminin; D010455:Peptides