Characterization of CD4+ T cell responses during natural infection with Salmonella typhimurium.
CD4+ T cells are important for resistance to infection with Salmonella typhimurium. However, the Ag specificity of this T cell response is unknown. Here, we demonstrate that a significant fraction of Salmonella-specific CD4+ T cells respond to the flagellar filament protein, FliC, and that this Ag has the capacity to protect naive mice from lethal Salmonella infection. To characterize this Ag-specific response further, we generated FliC-specific CD4+ T cell clones from mice that had resolved infection with an attenuated strain of Salmonella. These clones were found to respond to an epitope from a constant region of FliC, enabling them to cross-react with flagellar proteins expressed by a number of distinct Salmonella serovars.
McSorley
S J
SJ
Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USA. mcsor00@tc.umn.edu
Cookson
B T
BT
Jenkins
M K
MK
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000096:biosynthesis; D000954:Antigens, Surface; D015704:CD4 Antigens / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D002460:Cell Line; D002999:Clone Cells; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis; D005260:Female; D005408:Flagellin / Q000276:immunology; D016056:Immunodominant Epitopes / Q000276:immunology; D007127:Immunoglobulin Constant Regions / Q000276:immunology; D037181:Lectins, C-Type; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010446:Peptide Fragments / Q000276:immunology; D014176:Protein Biosynthesis; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis; D012481:Salmonella Infections, Animal / Q000276:immunology* / Q000382:microbiology* / Q000401:mortality / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000276:immunology* / Q000472:pathogenicity; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000382:microbiology
D000941:Antigens; D000954:Antigens, Surface; D015704:CD4 Antigens; D018984:Epitopes, T-Lymphocyte; D016056:Immunodominant Epitopes; D007127:Immunoglobulin Constant Regions; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010446:Peptide Fragments; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D005408:Flagellin