Cell-dependent replication potentials of HIV-1 gag mutants.
An infectious molecular clone of human immunodeficiency virus type 1 (HIV-1), designated pNLaiKH, which is tropic for both lymphocytic and monocytic cells, was constructed. To study the early function of HIV-1 Gag proteins in two types of cells, the mutations known to give host cell-dependent early defects were introduced into pNLaiKH, and the replication potentials and defective replication sites in the cells of the resultant mutants were monitored. All mutants grew in some lymphocytic cells, but not at all in monocytic cells. A nucleocapsid mutant was found to be defective at an early replication phase in all the cell lines to various extent, as expected. In contrast, a matrix mutant and a capsid mutant displayed a replication defect in a producer-cell-dependent manner. These results demonstrated that complex interactions of cell factors and Gag proteins are involved in an early process of HIV-1 replication.
Adachi
A
A
Department of Virology, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan.
Tamaki
M
M
Shimano
R
R
Inubushi
R
R
Naito
T
T
Yoshida
K
K
Oshima
Y
Y
Kawamura
M
M
Koyama
A H
AH
D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000821:virology*; D009000:Monocytes / Q000821:virology; D009154:Mutation; D019251:Nucleocapsid / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D020298:U937 Cells; D014779:Virus Replication
D015683:Gene Products, gag; D015500:Chloramphenicol O-Acetyltransferase; D012194:RNA-Directed DNA Polymerase