title: Molecular methods for the epidemiological typing of Salmonella enterica serotype Typhi from Hong Kong and Vietnam.
abstract: A total of 217 and 73 strains of Salmonella enterica serotype Typhi isolated from 1985 to 1997 in Hong Kong and in 2 months of 1989 and 1990 in Vietnam, respectively, were studied. These isolates were typed by plasmid profile analysis, plasmid fingerprinting, ribotyping with PstI, and total DNA fingerprinting with NarI. There appeared to be no major outbreak of typhoid fever in Hong Kong during the study period since there was considerable heterogeneity among the isolates. Isolates from Hong Kong were different from those from Vietnam. Thirty-seven percent of Vietnamese isolates belonged to two predominant clones, with the rest being heterogeneous in nature. Total DNA fingerprinting supplemented with ribotyping could be a reliable and rapid method for epidemiological typing of S. enterica serotype Typhi.
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, The Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. bo12765@mailserv.cuhk.edu.hk
authors.forename: J M
authors.initials: JM
authors.lastname: Ling
authors.forename: N W
authors.initials: NW
authors.lastname: Lo
authors.forename: Y M
authors.initials: YM
authors.lastname: Ho
authors.forename: K M
authors.initials: KM
authors.lastname: Kam
authors.forename: N T
authors.initials: NT
authors.lastname: Hoa
authors.forename: L T
authors.initials: LT
authors.lastname: Phi
authors.forename: A F
authors.initials: AF
authors.lastname: Cheng
mesh_terms: D015373:Bacterial Typing Techniques / Q000191:economics; D001434:Bacteriophage Typing; D016172:DNA Fingerprinting; D004275:DNA, Ribosomal / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004352:Drug Resistance, Microbial / Q000235:genetics; D006723:Hong Kong / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology / Q000191:economics / Q000379:methods; D010957:Plasmids; D015203:Reproducibility of Results; D012485:Salmonella typhi / Q000145:classification*; D012703:Serotyping; D013997:Time Factors; D014435:Typhoid Fever / Q000453:epidemiology / Q000382:microbiology*; D014744:Vietnam / Q000453:epidemiology
chemical_list: D004275:DNA, Ribosomal; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific