Substrate and donor specificity of glycosyl transferases.
It has been shown that all selectins recognize the carbohydrate epitopes sialyl Lewis(x) and sialyl Lewis(a). For the establishment of the structure-activity relationship, the efficient synthesis of these tetrasaccharides and derivatives is therefore of vital interest. The glycosyl transferase-mediated approach is summarized with emphasis on the use of modified acceptors and modified sugar-nucleotide donors. A survey of the involved enzymes: beta(1-3) and beta(1-4)galactosyl transferases, alpha(2-3)sialyl transferase, FucT III and FucT VI reveals that the enzymatic synthesis is highly efficient for the rapid preparation of sialyl Lewis(x)- and sialyl Lewis(a)-derivatives.
Ernst
B
B
University of Basel, Institute of Molecular Pharmacy, Switzerland. ERNSTB@ubaclu.unibas.ch
Oehrlein
R
R
D000818:Animals; D018395:CA-19-9 Antigen; D002240:Carbohydrate Sequence; D000939:Epitopes; D016695:Glycosyltransferases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis / Q000138:chemical synthesis*; D000080506:Sialyl Lewis X Antigen; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D018395:CA-19-9 Antigen; D000939:Epitopes; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D016695:Glycosyltransferases