A new optical immunoassay for detection of S-100B protein in whole blood.
BACKGROUND
S-100B is a protein mainly found in astroglial cells and only detected to a low level in blood. Serum levels of S-100B increase in patients with acute brain injuries. The aim of this study was to establish feasibility of a new Optical ImmunoAssay ([OIA], Bio-Star, Inc, Boulder, CO) test for determination of S-100B in blood.


METHODS
We have developed a new, rapid, and sensitive OIA test to identify elevated levels of S-100B in whole blood. The OIA test for S-100B combines monoclonal antibodies specific for the B-subunit of S-100 with OIA thin film technology. Each sample was tested for S-100B by the OIA method and a commercially available immunoluminometric assay. Blood samples were drawn serially from 9 patients undergoing coronary artery bypass graft surgery and during the early postoperative period.


RESULTS
The OIA test determination of S-100B protein correlated with immunoluminometric assay data (r = 0.8) with a detection limit of 0.25 ng/mL.


CONCLUSIONS
The sensitivity and feasibility of this rapid assay may be suitable for rapid evaluation of S-100B in urgent care settings (surgery, intensive care units, or emergency room).
Ettinger
A
A
BioStar, Inc, Boulder, Colorado 80301, USA. a_ettinger@biostar.com
Laumark
A B
AB
Ostroff
R M
RM
Brundell
J
J
Baumgartner
W A
WA
Razumovsky
A Y
AY
D000368:Aged; D002135:Calcium-Binding Proteins / Q000097:blood*; D002561:Cerebrovascular Disorders / Q000175:diagnosis / Q000209:etiology; D001026:Coronary Artery Bypass / Q000009:adverse effects; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D008297:Male; D009414:Nerve Growth Factors / Q000097:blood*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D012680:Sensitivity and Specificity
D002135:Calcium-Binding Proteins; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins