Interspersed repetitive sequence (IRS)-PCR for typing of whole genome radiation hybrid panels.
The typing of a radiation hybrid (RH) panel is generally achieved using a unique primer pair for each marker. We here describe a complementing approach utilizing IRS-PCR. Advantages of this technology include the use of a single universal primer to specify any locus, the rapid typing of RH lines by hybridization, and the conservative use of hybrid DNA. The technology allows the mapping of a clone without the requirement for STS generation. To test the technique, we have mapped 48 BAC clones derived from mouse chromosome 12 which we mostly identified using complex probes. As mammalian genomes are repeat-rich, the technology can easily be adapted to species other than mouse.
Himmelbauer
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Max-Planck-Institute of Molecular Genetics, Ihnestrasse 73, D-14195 Berlin-Dahlem, Germany. himmelbauer@molgen.mpg.de
Schalkwyk
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Lehrach
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D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D015723:Gene Library; D016678:Genome; D006801:Humans; D006822:Hybrid Cells; D020071:Interspersed Repetitive Sequences; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*