[The transfer of foreign DNA by rabbit spermatozoa into hamster eggs].
Capacitated rabbit sperm was incubated with pCMVlacZ plasmid and then used to fertilize hamster oocytes liberated from zona pellucida. After treatment with DNase I, these oocytes were examined for the presence of exogenous DNA by PCR. DNA of pCMVlacZ was not found in oocytes after simple incubation with male gametes. The presence of DNA was recorded in the experiments where the sperms were treated with DMSO and subjected to heat shock prior to fertilization.
Kuznetsov
A V
AV
State Scientific Center of Applied Microbiology, Obolensk, Moscow Oblast, Russia.
Shchit
I Iu
IIu
Kuznetsova
I V
IV
Dvorianchikov
G A
GA
D000818:Animals; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics; D004247:DNA / Q000187:drug effects / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D003850:Deoxyribonuclease I / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D018014:Gene Transfer Techniques; D008297:Male; D010063:Ovum / Q000187:drug effects / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics; D013075:Sperm Capacitation / Q000187:drug effects / Q000235:genetics; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D014162:Transfection / Q000187:drug effects / Q000235:genetics / Q000379:methods
D004274:DNA, Recombinant; D004247:DNA; D003850:Deoxyribonuclease I; D004121:Dimethyl Sulfoxide