Do quiescent human peripheral lymphocytes repair radiation-induced chromosome damage?
Peripheral blood samples from seven healthy donors were exposed to 2 Gy of (60)Co gamma rays. Lymphocyte cultures were prepared from the samples and stimulated to proliferate either immediately after the exposure or after 1, 2 or 4 h. No significant differences were found between the frequencies of chromosome aberrations (breaks or exchange) observed in lymphocytes stimulated after different postirradiation periods. We conclude that unstimulated lymphocytes do not undergo significant levels of recovery from potentially lethal damage at the chromosomal level.
Kondrashova
T V
TV
Medical Radiological Research Center, Russian Academy of Medical Sciences, Obninsk, 249020, Russia.
Ivanova
T I
TI
D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000235:genetics / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D013997:Time Factors