title: Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia.
abstract: Neurochemical and functional abnormalities of the striatum have been reported in schizophrenic brains, but the cellular substrates of these changes are not known. We hypothesized that schizophrenia may involve an abnormality in one of the key modulators of striatal output, the cholinergic interneuron. We measured the densities of cholinergic neurons in the striatum in schizophrenic and control brains in a blind analysis, using as a marker of this cell population immunoreactivity for choline acetyltransferase, the synthetic enzyme of acetylcholine. As an independent marker, we used immunoreactivity for calretinin, a protein which is co-localized with choline acetyltransferase in virtually all of the cholinergic interneurons of the striatum. A significant decrease in choline acetyltransferase-positive and calretinin-positive cell densities was found in the schizophrenic cases compared with controls in the striatum as a whole [for the choline acetyltransferase-positive cells: controls: 3.21 +/- 0.48 cells/mm2 (mean +/- S.D.), schizophrenics: 2.43 +/- 0.68 cells(mm2; P < 0.02]. The decrease was patchy in nature and most prominent in the ventral striatum (for the choline acetyltransferase-positive cells: controls: 3.47 +/- 0.59 cells/mm2, schizophrenics: 2.52 +/- 0.64 cells/ mm2; P < 0.005) which included the ventral caudate nucleus and nucleus accumbens region. Three of the schizophrenic cases with the lowest densities of cholinergic neurons had not been treated with neuroleptics for periods from more than a month to more than 20 years. A decrease in the number or function of the cholinergic interneurons of the striatum may disrupt activity in the ventral striatal-pallidal-thalamic-prefrontal cortex pathway and thereby contribute to abnormalities in function of the prefrontal cortex in schizophrenia.
authors.affiliation: Department of Neurology, Beth Israel Deaconess Medical Center and the Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Holt
authors.forename: M M
authors.initials: MM
authors.lastname: Herman
authors.forename: T M
authors.initials: TM
authors.lastname: Hyde
authors.forename: J E
authors.initials: JE
authors.lastname: Kleinman
authors.forename: C M
authors.initials: CM
authors.lastname: Sinton
authors.forename: D C
authors.initials: DC
authors.lastname: German
authors.forename: L B
authors.initials: LB
authors.lastname: Hersh
authors.forename: A M
authors.initials: AM
authors.lastname: Graybiel
authors.forename: C B
authors.initials: CB
authors.lastname: Saper
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001923:Brain Chemistry; D064032:Calbindin 2; D002452:Cell Count; D002795:Choline O-Acetyltransferase / Q000032:analysis; D002799:Cholinergic Fibers / Q000737:chemistry / Q000201:enzymology / Q000473:pathology*; D003342:Corpus Striatum / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007395:Interneurons / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D008875:Middle Aged; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D012559:Schizophrenia / Q000378:metabolism* / Q000473:pathology*
chemical_list: C576764:CALB2 protein, human; D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G; D002795:Choline O-Acetyltransferase