title: A solitary cutaneous tumor with distinct areas of verruca and seborrheic keratosis-like lesion.
abstract: A single, exophytic, cutaneous tumor on the thigh of a 52-year-old man was examined by light microscopy, in situ hybridization and immunohistochemistry. It demonstrated distinct areas of verruca and of seborrheic keratosis-like morphology simultaneously. Focally, architectural abnormalities were noted in some deeper parts of the tumor, but there was no morphological evidence of malignancy. The patient has remained disease-free for two and a half years after surgery. Biotinylated full genomicDNA probes of HPV confirmed the presence of types 6/11 exclusively in the verrucous portion of the neoplasm. In the verrucous component p53 protein was overexpressed and, additionally, increased Ki-67 immunopositive signals were detected, being localized below the HPV-DNA-expressing spinous cells.
authors.affiliation: The Medical School of the Athens National University, Department of Anatomic Pathology 75 Mikras Asias Str., Goudi, Athens, GR-115 27, Greece. alazaris@cc.uoa.gr.
authors.forename: A C
authors.initials: AC
authors.lastname: Lazaris
authors.forename: H
authors.initials: H
authors.lastname: Paraskevakou
authors.forename: P S
authors.initials: PS
authors.lastname: Davaris
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000473:pathology / Q000821:virology; D017492:Keratosis, Seborrheic / Q000150:complications / Q000473:pathology* / Q000821:virology; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification; D012871:Skin Diseases / Q000150:complications / Q000473:pathology* / Q000821:virology; D012878:Skin Neoplasms / Q000150:complications / Q000473:pathology* / Q000601:surgery / Q000821:virology; D016896:Treatment Outcome; D016159:Tumor Suppressor Protein p53 / Q000032:analysis; D014860:Warts / Q000150:complications / Q000473:pathology* / Q000821:virology
chemical_list: D004279:DNA, Viral; D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53