title: cDNA cloning and expression of a C-terminus motor kinesin-like protein KLP-17, involved in chromosomal movement in Caenorhabditis elegans.
abstract: Members of the kinesin protein family transport intracellular cargo to their correct cellular destination. Previously we have characterized the klp-3 gene from Caenorhabditis elegans, which encodes an ortholog of the retrograde C-terminus kinesin motors, such as Drosophila NCD, and yeast KAR3, involved in the chromosomal movement. Here we report the cloning of a full-length klp-17 cDNA in C. elegans, encoding a C-terminus kinesin of 605 amino residues. KLP-17 sequence defines a novel phylogenetic group, distinct from the NCD/KAR3 family. Interestingly, the klp-17 gene transcript is restricted to the nuclear compartment, as deduced by the RNA in situ hybridization in embryos. The klp-17::gfp-expressing transgenic animals do not display any GFP fluorescence signal, but expression of the extra chromosomal arrays cause production of abnormal males, and embryos with morphological defects and lethality in the progeny. Similarly, the klp-17 RNA interference assay results in embryonic death, arrested embryos, and polyploid cells. Thus, KLP-17 represents a new motor protein that mediates chromosome movement, essential for cell divisions during metazoan development.
authors.affiliation: Laboratory of Molecular Biology, Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, 441-8580, Japan.
authors.forename: M Y
authors.initials: MY
authors.lastname: Ali
authors.forename: S S
authors.initials: SS
authors.lastname: Siddiqui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics* / Q000502:physiology; D002455:Cell Division; D002874:Chromosome Mapping; D002875:Chromosomes / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D015870:Gene Expression; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics* / Q000502:physiology; D017403:In Situ Hybridization; D016547:Kinesins / Q000235:genetics* / Q000502:physiology; D008297:Male; D020409:Molecular Motor Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009068:Movement; D010802:Phylogeny
chemical_list: D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D020409:Molecular Motor Proteins; C404222:kinesin-like protein 17; D016547:Kinesins