title: Polymerase chain reaction assay to identify all immature stages of two species of the Anopheles quadrimaculatus sibling species complex (Diptera: Culicidae).
abstract: A polymerase chain reaction assay that detects differences in the 2nd internal transcribed spacer of ribosomal DNA was tested as a means to identify all immature stages of 2 species in the Anopheles quadrimaculatus sibling species complex. The technique was successful in amplifying DNA from eggs, 1st-4th instars, and pupae of An. quadrilmaculatus Say and An. inundatus Reinert.
authors.affiliation: Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge 70893, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Rutledge
authors.forename: D M
authors.initials: DM
authors.lastname: Wesson
authors.forename: C L
authors.initials: CL
authors.lastname: Meek
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D004275:DNA, Ribosomal / Q000032:analysis*; D005260:Female; D007303:Insect Vectors; D007814:Larva / Q000235:genetics; D010063:Ovum; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004275:DNA, Ribosomal