Nerve growth factor promoter driven neurotrophin-3 overexpression in the mouse and the protective effect of transgene on age-related behavioral deficits.
To clarify the biological function of neurotrophin-3 (NT-3) at the postnatal stage, we created a line of transgenic mice overexpressing NT-3 under the control of the mouse nerve growth factor gene promoter. Transgenic mice showed high-level NT-3 expression in the hippocampus and several tissues. We performed behavioral tests in young-adult (7-months-old) and aged (25-months-old) mice. Although aged non-transgenic mice exhibited spatial learning impairments in the Morris water maze, overexpression of NT-3 protected against these age-dependent spatial learning impairments in mice.
Kaisho
Y
Y
Discovery Research Division, Takeda Chemical Industries Ltd., Yodogawa, Osaka, Japan. kaisho_yoshihiko@takeda.co.jp
Ohta
H
H
Miyamoto
M
M
Igarashi
K
K
D000375:Aging / Q000523:psychology*; D000818:Animals; D006624:Hippocampus / Q000378:metabolism; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D020932:Nerve Growth Factor / Q000235:genetics*; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000502:physiology*; D019076:Transgenes / Q000502:physiology*
D020933:Neurotrophin 3; D020932:Nerve Growth Factor