DNA sequencing by capillary array electrophoresis with an electric field strength gradient.
We examined the effect of an electric field strength gradient on DNA sequencing efficiency using capillary array electrophoresis. Several types of gradients were applied to DNA sequencing and tested in terms of read length and accuracy. Our original method improved the accuracy of DNA sequencing for longer fragments at high temperature.
Endo
Y
Y
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi, Japan.
Yoshida
C
C
Baba
Y
Y
D004574:Electromagnetic Fields; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*