Stimulation of the GTPase activity of translation elongation factor G by ribosomal protein L7/12.
Elongation factors (EFs) Tu and G are GTPases that have important functions in protein synthesis. The low intrinsic GTPase activity of both factors is strongly stimulated on the ribosome by unknown mechanisms. Here we report that isolated ribosomal protein L7/12 strongly stimulates GTP hydrolysis by EF-G, but not by EF-Tu, indicating a major contribution of L7/12 to GTPase activation of EF-G on the ribosome. The effect is due to the acceleration of the catalytic step because the rate of GDP-GTP exchange on EF-G, as measured by rapid kinetics, is much faster than the steady-state GTPase rate. The unique, highly conserved arginine residue in the C-terminal domain of L7/12 is not essential for the activation, excluding an "arginine finger"-type mechanism. L7/12 appears to function by stabilizing the GTPase transition state of EF-G.
Savelsbergh
A
A
Institute of Molecular Biology, University of Witten/Herdecke, 58448 Witten, Germany.
Mohr
D
D
Wilden
B
B
Wintermeyer
W
W
Rodnina
M V
MV
D019943:Amino Acid Substitution; D001120:Arginine; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D008715:Methionine; D016297:Mutagenesis, Site-Directed; D020653:Peptide Elongation Factor G / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*
D020653:Peptide Elongation Factor G; D012360:RNA, Transfer, Phe; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12; D006160:Guanosine Triphosphate; D001120:Arginine; D008715:Methionine; D020558:GTP Phosphohydrolases