Site-specific formation of abasic lesions in DNA.
A method for the introduction of depurinated lesions in DNA is described, and is based on the formation of a covalent cross-link between an antisense oligonucleotide probe and the target DNA sequence followed by an unexpectedly mild thermal depurination.
Coleman
R S
RS
Department of Chemistry, Ohio State University, Columbus 43210-1185, USA.
Pires
R M
RM
D000081:Acetamides / Q000737:chemistry; D016375:Antisense Elements (Genetics); D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000737:chemistry; D006361:Heating; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D013891:Thiouridine / Q000031:analogs & derivatives / Q000737:chemistry
D000081:Acetamides; D016375:Antisense Elements (Genetics); D003432:Cross-Linking Reagents; D016376:Oligonucleotides, Antisense; C016272:N-bromoacetamide; D013891:Thiouridine; C004274:4-thio-2'-deoxyuridine; D004247:DNA; D003849:Deoxyguanosine