High-performance liquid chromatographic analysis of the D4 receptor antagonist SCH 66712 in rat plasma.
SCH 66712 is a potent and selective dopamine D4 receptor antagonist. An HPLC method was developed for the analysis of SCH 66712 in the plasma of rats, a species used for safety evaluation of this compound. The method involved solid-phase extraction on an ethyl cartridge and HPLC separation on a reversed-phase C8 column with quantitation using a fluorescence detector. The calibration curve was linear over a concentration range of 5-100 ng/ml. The limit of quantitation was 5 ng/ml, where the coefficient of variation (C.V.) was 2.9% and the bias was 6%. The precision of the method was satisfactory as indicated by an intra-day C.V. of < or = 4% and an inter-day C.V. of < or = 6%. The accuracy was also satisfactory as shown by an intra-day bias of < or = 8% and an inter-day bias of < or = 9%. The assay was shown to be sensitive, specific, accurate, precise, and reliable for use in pharmacokinetic or toxicokinetic studies.
Kim
H
H
Department of Drug Metabolism and Pharmacokinetics, Schering-Plough Research Institute, Kenilworth, NJ, USA.
Schuessler
D G
DG
Bach
C A
CA
Lin
C C
CC
Nomeir
A A
AA
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D065127:Dopamine D2 Receptor Antagonists; D005260:Female; D007093:Imidazoles / Q000097:blood*; D008297:Male; D011743:Pyrimidines / Q000097:blood*; D011786:Quality Control; D051381:Rats; D050638:Receptors, Dopamine D4; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D065127:Dopamine D2 Receptor Antagonists; C493601:Drd4 protein, rat; D007093:Imidazoles; D011743:Pyrimidines; C403229:SCH 66712; D050638:Receptors, Dopamine D4