Genotype profiles of rotavirus strains from children in a suburban community in Guinea-Bissau, Western Africa.
The P (VP4) and G (VP7) genotypes of 167 group A rotavirus strains obtained during the period 1996 to 1998 from 149 children living in a suburban community in Guinea-Bissau, western Africa, were determined by the reverse transcription-PCR technique. A total of nine combinations including five different P types and five different G types were identified. The globally common genotype pairs P[8], G1; P[4], G2; P[8], G3 and P[8], G4 were underrepresented in this study area. We found a substantial year-to-year variation in the occurrence of the genotype combinations. In 1996 and 1997, P[6], G2 was the most frequent, whereas P[8], G1 was more common in 1998. The unusual type P[9], G3 and a few mixed infections were detected. Sixteen percent of the rotavirus-positive samples were nontypeable.
Fischer
T K
TK
Centre for International Health, University of Bergen, 5021 Bergen, Norway. thea@dadlnet.dk
Steinsland
H
H
Molbak
K
K
Ca
R
R
Gentsch
J R
JR
Valentiner-Branth
P
P
Aaby
P
P
Sommerfelt
H
H
D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005838:Genotype; D006169:Guinea-Bissau; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus