title: The nitric oxide-producing properties of Solanum lyratum.
abstract: We examined the effect of Solanum lyratum Thunb. (Solanaceae) (SL) on the production of nitric oxide (NO). Stimulation of mouse peritoneal macrophages with SL after the treatment of recombinant interferon-gamma (rIFN-gamma) resulted in increased NO synthesis. SL had no effect on NO synthesis by itself. When SL was used in combination with rIFN-gamma, there was a marked cooperative induction of NO synthesis in a dose-dependent manner. The optimal effect of SL on NO synthesis was shown 6 h after treatment with rIFN-gamma. The increased production of NO from rIFN-gamma plus SL-stimulated cells was decreased by the treatment with staurosporin. In addition, synergy between rIFN-gamma and SL was mainly dependent on SL-induced tumor necrosis factor-alpha (TNF-alpha) secretion. All the preparations of SL were endotoxin free. The present results indicate that the capacity of SL to increase NO production from rIFN-gamma-primed mouse peritoneal macrophages is the result of SL-induced TNF-alpha secretion via the signal transduction pathway of PKC activation.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea. hmkim@med.wonkwang.ac.kr
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: E
authors.initials: E
authors.lastname: Li
authors.forename: Y S
authors.initials: YS
authors.lastname: Lyu
authors.forename: C Y
authors.initials: CY
authors.lastname: Hwang
authors.forename: N H
authors.initials: NH
authors.lastname: An
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004347:Drug Interactions; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D016166:Free Radical Scavengers / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D011994:Recombinant Proteins; D015398:Signal Transduction / Q000187:drug effects; D019311:Staurosporine / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma; D019311:Staurosporine