Metabolic profiling of oxylipins upon salicylate treatment in barley leaves--preferential induction of the reductase pathway by salicylate(1).
In barley leaves, 13-lipoxygenases (13-LOXs) are induced by salicylate (SA) and jasmonate. Here, we show by metabolic profiling that upon SA treatment, free linolenic acid and linoleic acid accumulate in a 10:1 ratio reflecting their relative occurrence in leaf tissues. Furthermore, 13-LOX-derived products are formed and specifically directed into the reductase branch of the LOX pathway leading mainly to the accumulation of (13S,9Z,11E,15Z)-13-hydroxy-9, 11,15-octadecatrienoic acid (13-HOT). Under these conditions, no accumulation of other products of the LOX pathway has been found. Moreover, exogenously applied 13-HOT led to PR1b expression suggesting for the time a role of hydroxy polyenoic fatty acid derivatives in plant defense reactions.
Weichert
H
H
Institute of Plant Biochemistry, Weinberg 3, D-06120, Halle/Saale, Germany.
Stenzel
I
I
Berndt
E
E
Wasternack
C
C
Feussner
I
I
D004790:Enzyme Induction; D001467:Hordeum / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008041:Linoleic Acids / Q000378:metabolism*; D008084:Lipoxygenase / Q000096:biosynthesis* / Q000378:metabolism; D010544:Peroxidases / Q000096:biosynthesis*; D054464:Peroxiredoxins; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D012459:Salicylates / Q000494:pharmacology*
D008041:Linoleic Acids; D012459:Salicylates; D010544:Peroxidases; D054464:Peroxiredoxins; C117433:13-lipoxygenase; D008084:Lipoxygenase