Impairment of neurogenic inflammatory and anti-inflammatory responses in diabetic rats.
The effect was studied of a primary (preconditioning) neurogenic inflammatory challenge induced by electrical stimulation of the peripheral stump of the sciatic nerve (20 V, 0.5 ms, 5 Hz, for 5 min) on neurogenic oedema (5 min later) induced by stimulation of the contralateral sciatic nerve. Plasma extravasation due to the second stimulation was decreased by 52.7+/-3.1% (P<0.01) in normal animals and by 29.7+/-2.2 and 18.1+/-1.5% with 50 mg/kg streptozotocin pretreatment i.v. 4 and 8 weeks previously, respectively. Subsequently, bilateral sciatic nerve stimulation increased baseline plasma somatostatin levels from 6.4+/-0.3, 11. 7+/-1.4, and 16.8+/-3.8 to 28.3+/-2.9 (P<0.01), 17.9+/-3.7, and 25. 1+/-1.7 pmol/l in normal, and 4- and 8-week diabetic animals, respectively. We conclude that experimental diabetes impairs the capability of a preconditioning neurogenic inflammatory episode to elicit a systemic anti-inflammatory effect. This is accompanied by a deficiency in elevation of the plasma somatostatin level in response to nerve stimulation, although the baseline plasma somatostatin level increases proportionally to the duration of experimental diabetes.
Németh
J
J
Neuropharmacology Research Group of the Hungarian Academy of Sciences, H-7601 P.O. Box 99, Pécs, Hungary.
Thán
M
M
Sári
R
R
Peitl
B
B
Oroszi
G
G
Farkas
B
B
Szolcsányi
J
J
Szilvássy
Z
Z
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003929:Diabetic Neuropathies / Q000503:physiopathology*; D004558:Electric Stimulation; D005070:Evans Blue; D005215:Fasting / Q000502:physiology; D007249:Inflammation / Q000503:physiopathology*; D007328:Insulin / Q000097:blood; D008297:Male; D009431:Neural Conduction / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000503:physiopathology*; D013004:Somatostatin / Q000097:blood
D001786:Blood Glucose; D007328:Insulin; D005070:Evans Blue; D013004:Somatostatin