Reactivity of singlet oxygen with tryptophan residues and with melittin in liposome systems.
The reactivity of singlet oxygen, 1O2, with amino acids, polypeptides and proteins has been studied extensively in solution, in micelles and also in vesicles. Here we attempt to examine its reactivity with N-acetyltryptophan amide (NATA), with a tryptophan residue with a long aliphatic chain attached, Trp(CH2)16, and with melittin--a small membrane protein--in a solution containing liposomes. In such a heterogeneous system the sensitizer and/or the tryptophan residue can be located in the ambient D2O, in the liposome membrane or at the membrane-solution interface. The sensitizer meso-tetra(N-methyl-4 pyridyl)porphine tetratosylate (mTPTT) is located in the aqueous phase while hematoporphyrin (HP) is embedded in the membrane. The quenching of 1O2 by the tryptophan residues and by melittin in solution, when using either of the sensitizers, was compared with the data in the liposome-containing system. It was found that the location of the sensitizer and of the quencher in the liposome membrane or in the surrounding solution greatly affects the quenching rate constants of 1O2.
Vilensky
A
A
Department of Physical Chemistry, Hebrew University, Jerusalem, Israel.
Feitelson
J
J
D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008555:Melitten / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry
D008081:Liposomes; D017319:Photosensitizing Agents; D011166:Porphyrins; D008555:Melitten; C016952:N-acetyltryptophanamide; C021096:tetra(4-N-methylpyridyl)porphine; D014364:Tryptophan; D010100:Oxygen