title: Effect of cycling temperatures on the production of deoxynivalenol and zearalenone by Fusarium graminearum NRRL 5883.
abstract: The effects of three regimens of cycling incubation temperatures and incubation at constant 25 degrees C on the growth of Fusarium graminearum NRRL 5883 and production of deoxynivalenol (DON) and zearalenone (ZEN) on rice were compared. The effects of low-temperature stress were also studied by incubating rice cultures at a constant 15 degrees C for 4 weeks following incubation at constant 25 degrees C for 2 weeks. Both incubation temperature and time significantly (P < or = 0.05) affected growth of F. graminearum NRRL 5883 and production of DON and ZEN. The highest amount of free ergosterol (640 microg/g culture material) that was used as a measure of fungal growth was found in cultures incubated at temperatures cycling between 15 and 30 degrees C during a 6-week period. The highest amounts of DON (1,679 microg/g culture material) and ZEN (603 microg/g culture material) were produced in cultures incubated at a constant 25 degrees C for 2 weeks prior to incubation at a constant 15 degrees C for an additional 4 weeks. Under cycling incubation temperatures, maximum amounts of DON (850 microg/g culture material) and ZEN (98 microg/g culture material) were produced in cultures incubated at temperatures cycling between 15 and 30 degrees C for 6 weeks. Overall, there was no correlation between mold growth and production of either DON or ZEN. However, DON production and ZEN production were correlated.
authors.affiliation: Department of Food Science and Technology, University of Nebraska-Lincoln, 68583, USA.
authors.forename: D
authors.initials: D
authors.lastname: Ryu
authors.forename: L B
authors.initials: LB
authors.lastname: Bullerman
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005670:Fusarium / Q000254:growth & development* / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology; D013696:Temperature; D014255:Trichothecenes / Q000096:biosynthesis*; D015025:Zearalenone / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D014255:Trichothecenes; D015025:Zearalenone; C007262:deoxynivalenol