Interleukin-8 stimulates placental prostacyclin production in preeclampsia.
OBJECTIVE
Our purpose was to determine placental interleukin (IL)-8 production and its correlation with the prostacyclin production in normal and preeclamptic pregnancies and to evaluate the beneficial effect of IL-8 on prostacyclin production.


METHODS
We determined 1) the in vitro production of IL-8 and prostacyclin by placental villous tissues from normal and preeclamptic pregnancies and 2) the production of prostacyclin by villous tissues from preeclampsia treated with recombinant human IL-8 (rhIL-8). IL-8 levels were measured by enzyme-linked immunosorbent assay and prostacyclin by radioimmunoassay of 6-keto PGF1alpha, the stable metabolite of prostacyclin.


RESULTS
1) Placental production of IL-8 and 6-keto PGF1alpha were significantly less in preeclampsia than in normal pregnancies, P<0.05. 2) Placental production of 6-keto PGF1alpha and IL-8 was significantly correlated in preeclampsia, P<0.01. 3) Placental tissues treated with IL-8 exhibited a concentration-dependent increase in 6-keto PGF1alpha production.


CONCLUSIONS
Placental tissues from preeclampsia produce significantly less IL-8 than tissues from normal pregnancies, which correlates with decreased prostacyclin production. IL-8 improves placental prostacyclin production in preeclampsia.
Wang
Y
Y
Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130, USA.
Baier
J
J
Adair
C D
CD
Lewis
D F
DF
Krueger
S
S
Kruger
T
T
Gurski
M
M
Brown
E
E
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000328:Adult; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
D016209:Interleukin-8; D015121:6-Ketoprostaglandin F1 alpha