High-performance liquid chromatography separation methods for the analysis of peptide nucleic acids.
An analytical analysis of peptide nucleic acids (PNAs) was carried out by reversed-phase HPLC using a solvent system comprised of aqueous trifluoroacetic acid and acetonitrile. A regression equation was obtained which represents the relationship of the molecular mass, sequence composition and retention time. This equation can be used to estimate the retention time of a known PNA under certain HPLC conditions. In addition to this equation, new HPLC conditions were also optimized which can be used for separation of pure PNAs.
Wei
Y
Y
Department of Chemistry, American University, Washington, DC 20016, USA.
Marino
M
M
Thompson
B
B
Girard
J E
JE
D000097:Acetonitriles; D001482:Base Composition; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D008970:Molecular Weight; D020135:Peptide Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D012997:Solvents; D014269:Trifluoroacetic Acid
D000097:Acetonitriles; D020135:Peptide Nucleic Acids; D012997:Solvents; D014269:Trifluoroacetic Acid; C032159:acetonitrile