Lack of a chromosomal copy of the circular rDNA plasmid of Entamoeba histolytica.
A number of small circular DNAs constitute a part of the genome of Entamoeba histolytica. Among them, the 24.5 kb circular DNA encoding rRNA (EhR1) is the most abundant. Pulsed field gel electrophoresis was used to determine if a chromosomal copy of EhR1 exists and what fraction of the total genome is circular. The results show that the chromosomes of E. histolytica are linear, and that no copy of EhR1 could be detected in any of the linear chromosomes.
Bagchi
A
A
School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.
Bhattacharya
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A
Bhattacharya
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S
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004270:DNA, Circular / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004748:Entamoeba histolytica / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*
D004270:DNA, Circular; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31