title: Immune dysfunction following trauma-haemorrhage: influence of gender and age.
abstract: Recent studies indicate that young female proestrus mice show an enhanced immune response following trauma-haemorrhage, as opposed to the immunodepression observed in males of comparable age. Testosterone is suggested as the cause of immunodepression in males, whereas oestradiol seems to be responsible for the enhanced immune response in females, however, sex hormone levels decrease with age. To determine if the sexual dimorphism in immune responses observed in young mice following trauma-haemorrhage changes with age, young (2-3 months) and aged (18-19 months) male and female CBA/J NIA mice were subjected to soft-tissue trauma (laparatomy) and haemorrhage (35+5 mmHg for 90 min and fluid resuscitation) or sham operation. Mice were killed 24 h later, and whole blood, as well as splenic and peritoneal macrophages (Mstraight phi) obtained. Plasma 17beta-oestradiol and free testosterone decreased in aged females and males, respectively. Mstraight phi from young females had enhanced IL-1beta and suppressed IL-10 production following trauma-haemorrhage, while aged females had unchanged production IL-1beta and IL-6 production and enhanced IL-10 release. In contrast, IL-1beta and IL-6 production by Mbeta from young males was suppressed and IL-10 production enhanced following trauma-haemorrhage, whereas Mstraight phi from aged males produced elevated levels of IL-1beta and IL-6 and suppressed levels of IL-10 following trauma-haemorrhage. Thus, the gender-related changes in the immune response to trauma-haemorrhage were reversed in aged mice.
authors.affiliation: Center for Surgical Research and Department of Surgery, Brown University School of Medicine and Rhode Island Hospital, Providence, RI, 02903, USA.
authors.forename: V
authors.initials: V
authors.lastname: Kahlke
authors.forename: M K
authors.initials: MK
authors.lastname: Angele
authors.forename: A
authors.initials: A
authors.lastname: Ayala
authors.forename: M G
authors.initials: MG
authors.lastname: Schwacha
authors.forename: W G
authors.initials: WG
authors.lastname: Cioffi
authors.forename: K I
authors.initials: KI
authors.lastname: Bland
authors.forename: I H
authors.initials: IH
authors.lastname: Chaudry
mesh_terms: D000367:Age Factors; D000375:Aging / Q000097:blood / Q000276:immunology*; D000818:Animals; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood*; D006470:Hemorrhage / Q000097:blood / Q000209:etiology / Q000276:immunology*; D007375:Interleukin-1 / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011863:Radioimmunoassay; D012737:Sex Factors; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014947:Wounds and Injuries / Q000097:blood / Q000150:complications / Q000276:immunology*
chemical_list: D012739:Gonadal Steroid Hormones; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D016753:Interleukin-10