Histamine increases [Ca(2+)](in) and activates Ca-K and nonselective cation currents in cultured human capillary endothelial cells.
We characterized the effects of histamine on intracellular Ca(2+) and activation of ionic currents in human capillary endothelial cells. Histamine produced both a transient and sustained increase in intracellular Ca(2+). The transient response was mediated largely through intracellular Ca(2+) release and the sustained response was due to extracellular Ca(2+) entry. The increase in intracellular Ca(2+) by histamine was not affected by the H2 blocker cimetidine. But was entirely blocked by the H1 antagonist diphenhydramine showing that the histamine response in these cells is mediated through the H1 receptor. A transient ionic current is coactivated with the histamine-induced increase in intracellular Ca(2+) and this current has several properties of a nonselective, Ca(2+) permeable, cation channel (NSC). The magnitude of the NSC current does not strictly correlate with intracellular Ca(2+) levels. A Ca(2+)-activated K(+) current (BKCA) is activated by the increase in intracellular Ca(2+) and this current is blocked by the selective BKCA blocker iberiotoxin.
Jow
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Cardiovascular Diseases, Wyeth-Ayerst Research, CN-8000, Princeton, NJ 08543, USA.
Numann
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R
D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D002196:Capillaries / Q000187:drug effects; D002478:Cells, Cultured; D004155:Diphenhydramine / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects*; D016257:Fura-2; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006801:Humans; D018408:Patch-Clamp Techniques; D010455:Peptides / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects*
D006634:Histamine H1 Antagonists; D010455:Peptides; D015221:Potassium Channels; C064719:iberiotoxin; D006632:Histamine; D004155:Diphenhydramine; D011188:Potassium; D002118:Calcium; D016257:Fura-2