title: Age-associated changes in mitochondrial parameters on peripheral human lymphocytes.
abstract: Among theories of aging, mitochondria are believed to be involved in senescence. Alterations of respiratory chain function and accumulation of various mitochondrial DNA mutations have been reported in mammalian postmitotic tissues. Because mitochondria have a central role in apoptosis and in adenosine triphosphate production, alteration of mitochondria function could contribute to immune senescence. We searched for alterations of mitochondrial parameters in peripheral lymphocytes with aging. Comparisons of respiratory chain activities of complex II+III, III, and IV were carried out in two populations of healthy volunteers with average ages of 35.3 +/- 6.7 years and 80.8 +/- 8.7 years. No difference was observed in complex IV activity between each group, whereas a significant decrease of complex II+III and a nonsignificant decrease of complex III activity were observed with aging. Alterations in mitochondrial functions can result from mutations in mitochondrial DNA (mtDNA), the most common being the 4977-bp deletion (mtDNA(-4977)). In either group we observed many deletions of mtDNA on peripheral blood lymphocytes by large-fragment polymerase chain reaction. This result suggests that alterations of respiratory chain activities observed with aging in lymphocytes could be the result of nuclear DNA dysfunction, with consequences on immune function (reduced responsiveness to antigen). Its possible implication on the recent observation of increased apoptosis of CD45RA+ RO- T cells with aging is discussed.
authors.affiliation: Equipe Postulante Centre National de la Recherche Scientifique 118, Faculté de Médecine, Limoges, France. drouet@unilim.fr
authors.forename: M
authors.initials: M
authors.lastname: Drouet
authors.forename: F
authors.initials: F
authors.lastname: Lauthier
authors.forename: J P
authors.initials: JP
authors.lastname: Charmes
authors.forename: P
authors.initials: P
authors.lastname: Sauvage
authors.forename: M H
authors.initials: MH
authors.lastname: Ratinaud
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics / Q000378:metabolism*; D042963:Electron Transport Complex II; D014450:Electron Transport Complex III / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006801:Humans; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010088:Oxidoreductases / Q000378:metabolism; D012016:Reference Values; D013385:Succinate Dehydrogenase / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology
chemical_list: D009097:Multienzyme Complexes; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III