Actin-binding cellular proteins inside human immunodeficiency virus type 1.
Host proteins are incorporated both on and inside human immunodeficiency virus type 1 (HIV-1) virions. To identify cellular proteins inside HIV-1, virion preparations were treated by a protease-digestion technique that removes external host proteins, allowing for the study of the proteins inside the virus. Treated HIV-1 preparations were analyzed by immunoblot, high-pressure liquid chromatography, and protein sequence analyses. These analyses identified several cellular proteins inside HIV-1: elongation factor 1alpha, glyceraldehyde-3-phosphate dehydrogenase, HS-1, phosphatidylethanolamine-binding protein, Pin1, Lck, Nm23-H1, and the C-terminal tail of CD43. Several of these proteins were found as fragments of their full-sized proteins that appear to be generated by our protease treatment of the virions, the HIV-1 protease, or a cellular protease. Recent advances in cell biology and biochemistry have identified some of these proteins as actin-binding proteins. These results support the hypothesis that actin filaments are incorporated into the virion and may provide additional clues for the understanding of the interaction between viral and cellular proteins during assembly and budding.
Ott
D E
DE
SAIC Frederick, National Cancer Institute, Frederick, Maryland, 21702-1201, USA. ott@avpvx1.ncifcrf.gov
Coren
L V
LV
Johnson
D G
DG
Kane
B P
BP
Sowder
R C
RC
Kim
Y D
YD
Fisher
R J
RJ
Zhou
X Z
XZ
Lu
K P
KP
Henderson
L E
LE
D000199:Actins / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D018834:Chaperonin 60 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D016333:HIV Protease / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008840:Microfilament Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D020648:Peptide Elongation Factor 1 / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D049488:Phosphatidylethanolamine Binding Protein; D049294:Phospholipid Transfer Proteins; D020539:Sequence Analysis, Protein; D020860:Subtilisin / Q000378:metabolism; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D014771:Virion / Q000737:chemistry / Q000378:metabolism
D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D000727:Androgen-Binding Protein; D001798:Blood Proteins; D002352:Carrier Proteins; D018834:Chaperonin 60; C061909:HCLS1 protein, human; D008840:Microfilament Proteins; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D054778:NM23 Nucleoside Diphosphate Kinases; C511482:PEBP1 protein, human; D020648:Peptide Elongation Factor 1; D049488:Phosphatidylethanolamine Binding Protein; D049294:Phospholipid Transfer Proteins; D014157:Transcription Factors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020860:Subtilisin; D016333:HIV Protease; D020559:Monomeric GTP-Binding Proteins; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase