title: Increased interleukin-1beta concentration in gingival crevicular fluid as a characteristic of periodontitis.
abstract: BACKGROUND
  The levels of interleukin-1beta (IL-1beta) have been reported to be higher in sites with periodontitis than in healthy controls. This may be the result of a more severe inflammation and/or constitutional differences in IL-1beta production. Our aim was to test the hypothesis that the level of IL-1beta in gingival crevicular fluid (GCF) is a characteristic trait of periodontitis, regardless of the degree of tissue destruction. As a secondary aim, we investigated the correlation between IL-1beta and neutrophil elastase. An untreated population was used.
  
  
  METHODS
  GCF samples from inflamed sites in 13 patients with gingivitis (GG), and from inflamed sites with shallow (GP) and deep (PP) pockets in 18 patients with periodontitis were collected. Interleukin-1beta, elastase-alpha-1-antitrypsin complex (E-A1AT), alpha-1-antitrypsin (A1AT), and alpha-2-macroglobulin (A2MG) were measured with ELISA. Elastase activity was measured with a specific substrate.
  
  
  RESULTS
  The concentration of IL-1beta was significantly higher in PP than in GG, but no significant differences were detected between PP and GP. A weak positive correlation between the elastase activity and IL-1beta was also observed. The elastase activity had a tendency to be higher in PP, although no significant difference was found among the 3 groups. The concentrations of E-A1AT, A1AT, and A2MG were similar in the 3 types of sites.
  
  
  CONCLUSIONS
  The levels of IL-1beta in GCF were increased in samples from periodontitis patients, regardless of the severity of disease at the sampled site, suggesting that the levels of IL-1beta are typical of a given patient.
authors.affiliation: Department of Medical Laboratory Sciences, Karolinska Institute, Huddinge, Sweden. Carlos.da.silva.figueredo@ofa.ki.se
authors.forename: C M
authors.initials: CM
authors.lastname: Figueredo
authors.forename: M S
authors.initials: MS
authors.lastname: Ribeiro
authors.forename: R G
authors.initials: RG
authors.lastname: Fischer
authors.forename: A
authors.initials: A
authors.lastname: Gustafsson
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology; D005891:Gingivitis / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D019272:Leukocyte Elastase / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D010518:Periodontitis / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
chemical_list: D007375:Interleukin-1; D000515:alpha 1-Antitrypsin; C037741:alpha 1-antitrypsin-leukocyte elastase complex; D000511:alpha-Macroglobulins; D019272:Leukocyte Elastase