title: Electrogenicity of the Na+-ATPase from the marine microalga Tetraselmis (Platymonas) viridis and associated H+ countertransport.
abstract: Sodium accumulation by the Na+-ATPase in the plasma membrane (PM) vesicles isolated from the marine alga Tetraselmis (Platymonas) viridis was shown to be accompanied by deltapsi generation across the vesicle membrane (positive inside) and H+ efflux from the vesicle lumen. Na+ accumulation was assayed with 22Na+; deltapsi generation was detected by recording absorption changes of oxonol VI; H+ efflux was monitored as an increase in fluorescence intensity of the pH indicator pyranine loaded into the vesicles. Both ATP-dependent Na+ uptake and H+ ejection were increased by the H+ ionophore carbonyl cyanide m-chlorophenylhydrazone (CICCP) while deltapsi was collapsed. The lipophilic anion tetraphenylboron ion (TPB-) inhibited H+ ejection from the vesicles and abolished deltapsi. Based on the effects of CICCP and TPB- on H+ ejection and deltapsi generation, the conclusion was drawn that H+ countertransport observed during Na+-ATPase operation is a secondary event energized by the electric potential which is generated in the course of Na+ translocation across the vesicle membrane. Increasing Na+ concentrations stimulated H+ efflux and caused the decrease in the deltapsi observed, thus indicating that Na+ is likely a factor controlling H+ permeability of the vesicle membrane.
authors.affiliation: Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow.
authors.forename: Y V
authors.initials: YV
authors.lastname: Balnokin
authors.forename: L G
authors.initials: LG
authors.lastname: Popova
authors.forename: I M
authors.initials: IM
authors.lastname: Andreev
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000201:enzymology; D004594:Electrophysiology; D056890:Eukaryota / Q000201:enzymology* / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D012964:Sodium / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers / Q000502:physiology*; D013775:Tetraphenylborate / Q000378:metabolism; D013997:Time Factors; D014638:Vanadates / Q000378:metabolism
chemical_list: D027682:Cation Transport Proteins; D017923:Sodium-Hydrogen Exchangers; D014638:Vanadates; D013775:Tetraphenylborate; D000255:Adenosine Triphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase