Development of membranes for the cultivation of kidney epithelial cells.
The development of biohybrid organs (BHO) will benefit from improved membranes regarding transport and cell contacting properties. Here we describe in a first study the development and testing of membranes made of polyacrylonitrile (PAN) and polysulfone (PSU) for the immobilisation of kidney epithelial cells. Comparative investigations on overall polymer toxicity tested with 3T3 fibroblasts, and morphology and proliferation of Madin-Darby canine kidney (MDCK) cells cultured on the membranes could show that these materials have comparable cell contacting properties like Millicell membranes. Since PAN and PSU have superior membrane forming properties with regard to membrane geometry, i.e. for the preparation of hollow fibres, and porosity, i.e. for immuno isolation, both materials or modifications thereof seem to be suitable for the application in BHO such as biohybrid kidney.
Fey-Lamprecht
F
F
Institut für Pathologie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Germany. frederique.fey@gkss.de
Groth
T
T
Albrecht
W
W
Paul
D
D
Gross
U
U
D016475:3T3 Cells / Q000187:drug effects; D000180:Acrylic Resins; D000818:Animals; D001672:Biocompatible Materials; D002455:Cell Division; D002460:Cell Line; D004285:Dogs; D004847:Epithelial Cells / Q000502:physiology / Q000648:ultrastructure*; D007668:Kidney / Q000502:physiology / Q000648:ultrastructure*; D007688:Kidneys, Artificial; D008567:Membranes, Artificial; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D011108:Polymers; D016062:Porosity; D013450:Sulfones
D000180:Acrylic Resins; D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C010504:polyacrylonitrile; C017662:polysulfone P 1700