Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
The higher plant Arabidopsis thaliana (Arabidopsis) is an important model for identifying plant genes and determining their function. To assist biological investigations and to define chromosome structure, a coordinated effort to sequence the Arabidopsis genome was initiated in late 1996. Here we report one of the first milestones of this project, the sequence of chromosome 4. Analysis of 17.38 megabases of unique sequence, representing about 17% of the genome, reveals 3,744 protein coding genes, 81 transfer RNAs and numerous repeat elements. Heterochromatic regions surrounding the putative centromere, which has not yet been completely sequenced, are characterized by an increased frequency of a variety of repeats, new repeats, reduced recombination, lowered gene density and lowered gene expression. Roughly 60% of the predicted protein-coding genes have been functionally characterized on the basis of their homology to known genes. Many genes encode predicted proteins that are homologous to human and Caenorhabditis elegans proteins.
Mayer
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GSF-Forschungszentrum f. Umwelt u. Gesundheit, Munich Information Center for Protein Sequences am Max-Planck-Institut f. Biochemie, Germany.
Schüller
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Wambutt
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Murphy
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Volckaert
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Pohl
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Düsterhöft
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Stiekema
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Entian
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Terryn
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Harris
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Ansorge
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Brandt
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Grivell
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Rieger
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Weichselgartner
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de Simone
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Obermaier
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Mache
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Müller
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Kreis
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Delseny
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Puigdomenech
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Watson
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Schmidtheini
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Reichert
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Portatelle
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Perez-Alonso
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Boutry
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Bancroft
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Vos
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Hoheisel
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Zimmermann
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Wedler
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Ridley
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Langham
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McCullagh
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Bilham
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Robben
J
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Van der Schueren
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Grymonprez
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Chuang
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Vandenbussche
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Braeken
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Weltjens
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Voet
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Bastiaens
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Aert
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Defoor
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Weitzenegger
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Bothe
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Ramsperger
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Hilbert
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Braun
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Holzer
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Brandt
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Peters
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van Staveren
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Dirske
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Mooijman
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Klein Lankhorst
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Rose
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Hauf
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Kötter
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Berneiser
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Hempel
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Feldpausch
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Lamberth
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Van den Daele
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De Keyser
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Buysshaert
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Gielen
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Villarroel
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De Clercq
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Van Montagu
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Rogers
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Cronin
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Quail
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Bray-Allen
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Clark
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Doggett
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Hall
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Kay
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Lennard
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McLay
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Mayes
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Pettett
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Benes
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Rechmann
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Borkova
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Blöcker
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Scharfe
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Grimm
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Löhnert
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Dose
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de Haan
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Maarse
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Schäfer
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Müller-Auer
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Gabel
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Fuchs
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Fartmann
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Granderath
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Dauner
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Herzl
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Neumann
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Argiriou
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Vitale
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Liguori
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Piravandi
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Massenet
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Quigley
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Clabauld
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Mündlein
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Felber
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Schnabl
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Hiller
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Schmidt
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Lecharny
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Aubourg
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Chefdor
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Cooke
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Berger
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Montfort
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Casacuberta
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Gibbons
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Weber
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Vandenbol
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M
Bargues
M
M
Terol
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Torres
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Perez-Perez
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Purnelle
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Bent
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Johnson
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Tacon
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Jesse
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Heijnen
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Schwarz
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Scholler
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P
Heber
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Francs
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P
Bielke
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Frishman
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Haase
D
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Lemcke
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K
Mewes
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Stocker
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Zaccaria
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P
Bevan
M
M
Wilson
R K
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de la Bastide
M
M
Habermann
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K
Parnell
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Dedhia
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Gnoj
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Schutz
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Huang
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Spiegel
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Sehkon
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Murray
J
J
Sheet
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P
Cordes
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Abu-Threideh
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Stoneking
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Kalicki
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Graves
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Harmon
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Edwards
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Latreille
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Courtney
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Cloud
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Abbott
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Scott
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Johnson
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Minx
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Bentley
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Fulton
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Miller
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Greco
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Kemp
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Kramer
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Fulton
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Mardis
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Dante
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Pepin
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Hillier
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Nelson
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Spieth
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Ryan
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Andrews
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Geisel
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Layman
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Du
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Ali
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Berghoff
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Jones
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Drone
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Cotton
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Joshu
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Antonoiu
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Zidanic
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Strong
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Sun
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Lamar
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Yordan
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Zhong
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Preston
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Vil
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Shekher
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Matero
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Shah
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R
Swaby
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O'Shaughnessy
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Rodriguez
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Hoffmann
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Till
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Granat
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Shohdy
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Hasegawa
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Hameed
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Lodhi
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M
Johnson
A
A
Chen
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E
Marra
M
M
Martienssen
R
R
McCombie
W R
WR
D000818:Animals; D017360:Arabidopsis / Q000235:genetics*; D002875:Chromosomes; D002894:Chromosomes, Human, Pair 4; D018744:DNA, Plant; D017343:Genes, Plant / Q000502:physiology; D006570:Heterochromatin; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D011487:Protein Conformation; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018744:DNA, Plant; D006570:Heterochromatin; D010940:Plant Proteins