Effects of amino acid substitutions at conserved and acidic residues within region 1.1 of Escherichia coli sigma(70).
Amino acid substitutions in Escherichia coli sigma(70) were generated and characterized in an analysis of the role of region 1.1 in transcription initiation. Several acidic and conserved residues are tolerant of substitution. However, replacement of aspartic acid 61 with alanine results in inactivity caused by structural and functional thermolability.
Bowers
C W
CW
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Houston, Texas 77030, USA.
McCracken
A
A
Dombroski
A J
AJ
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D012329:RNA, Bacterial / Q000096:biosynthesis; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic; D014357:Trypsin / Q000378:metabolism
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D014357:Trypsin