title: Effect of culture conditions on morphological changes of Helicobacter pylori.
abstract: The morphological conversion of Helicobacter pylori from the spiral form to the coccoid form may be the expression of a transitory adaptation to an unsuitable environment. The mechanism(s) of this conversion are not clear. In this study, we examined whether the morphological conversion of H. pylori is affected by various culture conditions, such as oxygen concentration, pH, temperature, or the presence of beta-cyclodextrin. H. pylori (NTCC11916) was cultured on Brucella agar, followed by culture in Brucella broth containing 1% agar under several conditions. Morphological conversion of individual H. pylori on the agar plate was investigated with time after incubation under phase contrast microscopy. When H. pylori was inoculated in Brucella broth containing beta-cyclodextrin, the spiral form of the organism was observed even after 6 days of incubation under standard culture conditions: 37 degrees C, pH 7, and microaerobic atmosphere (5% O2/10% CO2/85% N2) (control). The morphological conversion of H. pylori was completed on day 3 in an aerobic atmosphere (20% O2 supply) and on day 2 in an undermicroaerobic atmosphere (<0.1% O2). Its complete morphological conversion was observed at pH 8 on day 5 and at pH 4 on day 6. All of the H. pylori (100%) incubated at 20 degrees or 42 degrees C had converted from the bacillary to the coccoid form on day 4. Conditioned medium without beta-cyclodextrin caused complete conversion on day 5. These results suggest that oxygen concentration, pH, temperature, and beta-cyclodextrin may be related to the H. pylori morphological conversion from the bacillary to the coccoid form.
authors.affiliation: Third Department of Internal Medicine, Osaka City University Medical School, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tominaga
authors.forename: N
authors.initials: N
authors.lastname: Hamasaki
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: Y
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: O
authors.initials: O
authors.lastname: Takaishi
authors.forename: K
authors.initials: K
authors.lastname: Higuchi
authors.forename: T
authors.initials: T
authors.lastname: Arakawa
authors.forename: E
authors.initials: E
authors.lastname: Ishii
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: I
authors.initials: I
authors.lastname: Yano
authors.forename: T
authors.initials: T
authors.lastname: Kuroki
mesh_terms: D003470:Culture Media; D003505:Cyclodextrins; D016480:Helicobacter pylori / Q000254:growth & development*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen; D013696:Temperature; D013997:Time Factors; D047392:beta-Cyclodextrins
chemical_list: D003470:Culture Media; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; C031215:betadex; D010100:Oxygen