title: Correction of the DNA sequence of the regB gene of Rhodobacter capsulatus with implications for the membrane topology of the sensor kinase regB.
abstract: We corrected the previously published sequence for the regB gene, which encodes a histidine sensor kinase in Rhodobacter capsulatus. The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains.
authors.affiliation: Institut für Mikrobiologie und Molekularbiologie, D-35392 Giessen, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Jäger
authors.forename: G
authors.initials: G
authors.lastname: Klug
mesh_terms: D000469:Alkaline Phosphatase; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002462:Cell Membrane / Q000201:enzymology; D018844:Cyclin-Dependent Kinases / Q000235:genetics; D007763:Lac Operon; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D011494:Protein Kinases; D017433:Protein Structure, Secondary; D011993:Recombinant Fusion Proteins / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D011993:Recombinant Fusion Proteins; C091081:RegB protein, Rhodobacter; D011494:Protein Kinases; D018844:Cyclin-Dependent Kinases; D000469:Alkaline Phosphatase