Lineage specificity of CBFA2 fusion transcripts.
The CBFA2 gene on chromosome band 21q22 is one of the most commonly translocated genes in leukemia. As with other translocations, those involving CBFA2 are associated with specific disease phenotypes. Only one of the different translocations involving CBFA2, the t(12;21), has been associated with a non-myeloid lineage. Several different CBFA2 fusion transcripts were expressed in the myeloid 32Dcl3 cell line, and show that unlike the myeloid specific fusion transcripts, the lymphoid specific ETV6/CBFA2 transcript is not compatible with myeloid cell differentiation. It is shown that myeloid cells expressing the ETV6/CBFA2 transcript undergo apoptosis in response to a G-CSF differentiation signal. The molecular differences in the cells we studied are characterized using Western blot analysis to show that t(12;21) expressing cells fail to express the G-CSF receptor.
Dann
E J
EJ
Rambam Medical Center, Haifa, Israel.
Fears
S
S
Arád-Dann
H
H
Nucifora
G
G
Rowley
J D
JD
D000208:Acute Disease; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics* / Q000648:ultrastructure; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics* / Q000648:ultrastructure; D050676:Core Binding Factor Alpha 2 Subunit; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D009195:Peroxidase / Q000032:analysis; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000172:deficiency* / Q000235:genetics; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D000097764:ETS Translocation Variant 6 Protein
D050676:Core Binding Factor Alpha 2 Subunit; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016179:Granulocyte Colony-Stimulating Factor; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D009195:Peroxidase; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D014157:Transcription Factors; C107844:AML1-ETO fusion protein, human; C403143:CBFA2-EAP fusion protein, human; C403142:CBFA2-MDS1 protein, human; C493728:RUNX1 protein, human; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein