DNA analysis of a transplanted cryopreserved meniscal allograft.
Meniscal transplantation is frequently performed in young patients with a single meniscal-deficient compartment as a result of previous total meniscectomy. Indications, operative techniques, and preservation of meniscal allografts have been studied extensively. In this study we compared the DNA profile of a meniscal allograft with that of the human recipient 1 year after transplantation. Applying techniques routinely used in forensic analysis, we were able to show that the DNA profile of the meniscal allograft was 95% identical to that of the human recipient. These findings indicate that 1 year after transplantation the meniscal allograft is nearly completely repopulated by host cells.
Debeer
P
P
Department of Orthopaedic Surgery, University Hospital Pellenberg, Leuven, Belgium.
Decorte
R
R
Delvaux
S
S
Bellemans
J
J
D000328:Adult; D001182:Arthroscopy; D002357:Cartilage Diseases / Q000209:etiology / Q000601:surgery*; D003094:Collagen / Q000235:genetics*; D015925:Cryopreservation; D004247:DNA / Q000032:analysis*; D006801:Humans; D007718:Knee Injuries / Q000150:complications / Q000601:surgery; D008297:Male; D008592:Menisci, Tibial / Q000378:metabolism / Q000637:transplantation; D016133:Polymerase Chain Reaction; D014184:Transplantation, Homologous
D003094:Collagen; D004247:DNA