title: Evidence for an inactive transposable mariner-like element in Anopheles albimanus.
abstract: Transposable elements may eventually be used as transformation vectors of anti-Plasmodium genes in natural populations of Anopheles mosquitoes. The mariner transposable element is widespread in most animal phyla and we wished to test for its presence in the important Latin American malaria vector, Anopheles albimanus. Degenerate primers were designed from the consensus of insect mariner elements and used in a polymerase chain reaction to amplify a fragment of the predicted size from An. albimanus. The fragment was cloned, sequenced, and determined to be a mariner-like element through multiple alignment with known insect mariner elements. Dot blot and Southern blot analyses showed only one or a few elements per haploid genome. The cloned fragment was used as a probe to isolate similar sequences from an An. albimanus genomic DNA library. A 659-base-pair clone was 57% similar at the DNA level with mariner elements from Anopheles gambiae, Chrysoperla ptorapunda, and Haematobia irritans. This low sequence similarity is comparable to that found among mariner-like elements in all insects. However, conserved amino acid motifs were not discovered nor was a single open reading frame found. These aspects suggest that the An. albimanus mariner-like element may represent an ancient transposition event but that the element is no longer active, the typical open reading frame having been disrupted through nucleotide insertions and deletions.
authors.affiliation: Institute of Biotechnology, National Autonomous University of Mexico, Morelos.
authors.forename: K
authors.initials: K
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Lizardi
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: M H
authors.initials: MH
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004251:DNA Transposable Elements / Q000235:genetics*; D007303:Insect Vectors / Q000235:genetics; D008288:Malaria / Q000635:transmission; D010802:Phylogeny
chemical_list: D004251:DNA Transposable Elements; D004247:DNA