Interaction between GADD34 and kinesin superfamily, KIF3A.
GADD34 is one of a subset of proteins induced after DNA damage or cell growth arrest. To examine the function of GADD34, we used the yeast two-hybrid system to clone the protein that interacts with the murine GADD34. One cDNA clone was the C-terminal part of KIF3A gene including the tail domain. The interaction between GADD34 and KIF3A was confirmed in the NIH3T3 cells by in vivo two-hybrid analysis. We could detect that GADD34 was induced with methyl methanesulfonate; however, the mRNA induction of KIF3A was not detected.
Hasegawa
T
T
Department of Basic Gerontology, National Institute for Longevity Sciences, 36-3, Gengo Morioka-cho, Obu, Aichi, 474-8522, Japan.
Yagi
A
A
Isobe
K
K
D016475:3T3 Cells; D000818:Animals; D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; D002455:Cell Division; D003001:Cloning, Molecular; D004249:DNA Damage; D018076:DNA, Complementary / Q000235:genetics; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D054645:Protein Phosphatase 1; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C490202:Kif3a protein, mouse; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C513256:Ppp1r15a protein, mouse; D054645:Protein Phosphatase 1; D016547:Kinesins