Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae.
To gain better knowledge of the variety of digestive enzymes in phytophagous coleopteran pests, a sequencing screen of 76 random cDNAs from a gut library from Phaedon cochleariae larvae was performed. The screen yielded 21 cDNAs encoding amino-acid sequences homologous to known digestive enzymes, most of them were cell wall-hydrolysing enzymes. The deduced protein sequences of 7 cDNAs encoding putative alpha-amylase, cysteine proteinase, trypsin, chymotrypsin, cellulase, pectinase and xylanase display all the structural features that characterize these enzymes in other eukaryotic organisms. Except the alpha-amylase and chymotrypsin cDNAs, the other cDNAs probably derive from multigene families. The distribution of the corresponding enzymatic activities at various developmental stages of P. cochleariae was examined. alpha-amylase activity is present in guts of larvae and adults, proteinases are abundant in guts of larvae and adults, but scarce in eggs and larval carcasses, xylanases are present in the guts of larvae and adults, as well as in carcasses of larvae, whereas cellulase and pectinase activities are distributed in larval and adult guts, larval carcasses, and eggs. Only a minor fraction of the cellulases is secreted by microorganisms, suggesting that P. cochleariae synthesizes most of its own cell-wall hydrolysing enzymes. The physiological role of the enzymes is discussed, as well as the significance of these results for pest management strategies involving transgenic plants expressing enzyme inhibitors.
Girard
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C
Laboratoire de Biologie Cellulaire, INRA de Versailles, Versailles, France.
Jouanin
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L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D056668:Cathepsin L; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism; D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D002918:Chymotrypsin / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D001517:Coleoptera / Q000201:enzymology* / Q000235:genetics; D003546:Cysteine Endopeptidases; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011096:Polygalacturonase / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012967:Sodium Dodecyl Sulfate; D014357:Trypsin / Q000235:genetics / Q000378:metabolism; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D004798:Enzymes; D012967:Sodium Dodecyl Sulfate; D014995:Xylosidases; D000516:alpha-Amylases; D011096:Polygalacturonase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase; D002403:Cathepsins; D010450:Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; C534280:Ctsl protein, rat