Tanshinone IIA isolated from Salvia miltiorrhiza BUNGE induced apoptosis in HL60 human premyelocytic leukemia cell line.
Apoptosis is a new therapeutic target of cancer research. Tanshinone IIA isolated from Salvia miltiorrhiza BUNGE, a traditional oriental medical herb, was observed to induce apoptosis in HL60 human premyelocytic leukemia cell line. Tanshinone IIA induced DNA fragmentation into the multiples of 180 bp and increased the percentage of hypodiploid cells in flow cytometry after propidium iodide (PI) staining. Tanshinone IIA-induced apoptosis is accompanied by the specific proteolytic cleavage of poly(ADP-ribose) polymerase (PARP) and the activation of caspase-3, a major component in apoptotic cell death mechanism.
Yoon
Y
Y
Laboratory of Cancer Research, Korea Institute of Oriental Medicine, Seoul, South Korea. ysyoon66@hanmail.net
Kim
Y O
YO
Jeon
W K
WK
Park
H J
HJ
Sung
H J
HJ
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry; D053938:DNA Fragmentation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000188:drug therapy* / Q000473:pathology*; D010616:Phenanthrenes / Q000627:therapeutic use*; D010936:Plant Extracts / Q000627:therapeutic use*; D011065:Poly(ADP-ribose) Polymerases / Q000187:drug effects; D011419:Propidium / Q000737:chemistry; D013997:Time Factors; D014407:Tumor Cells, Cultured
D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D010936:Plant Extracts; C021751:tanshinone; D011419:Propidium; D011065:Poly(ADP-ribose) Polymerases; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases