title: Biological dosimetry for astronauts: a real challenge.
abstract: Manned space missions recently increased in number and duration, thus it became important to estimate the biological risks encountered by astronauts. They are exposed to cosmic and galactic rays, a complex mixture of different radiations. In addition to the measurements realized by physical dosimeters, it becomes essential to estimate real biologically effective doses and compare them to physical doses. Biological dosimetry of radiation exposures has been widely performed using cytogenetic analysis of chromosomes. This approach has been used for many years in order to estimate absorbed doses in accidental or chronic overexposures of humans. In addition to conventional techniques (Giemsa or FPG staining, R- or G-banding), faster and accurate means of analysis have been developed (fluorescence in situ hybridization [FISH] painting). As results accumulate, it appears that strong interindividual variability exists in the basal level of aberrations. Moreover, some aberrations such as translocations exhibit a high background level. Radiation exposures seem to induce variability between individual responses. Its extent strongly differs with the mode of exposure, the doses delivered, the kind of radiation, and the cytogenetic method used. This paper aims to review the factors that may influence the reliability of cytogenetic dosimetry. The emphasis is on the exposure to high linear energy transfer (LET) particles in space as recent studies demonstrated interindividual variations in doses estimated from aberration analysis after long-term space missions. In addition to the problem of dose estimates, the heterogeneity of cosmic radiation raises questions relating to the real numbers of damaged cells in an individual, and potential long-term risks. Actually, densely ionizing particles are extremely potent to induce late chromosomal instability, and again, interindividual variability exists in the expression of damage.
authors.affiliation: CEA, Commissariat a l'Energie Atomique, DSV/DRR, Laboratoire de Radiobiologie et Oncologie, BP6, Fontenay-aux-Roses, France.
authors.forename: I
authors.initials: I
authors.lastname: Testard
authors.forename: L
authors.initials: L
authors.lastname: Sabatier
mesh_terms: D018480:Astronauts; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects*; D003359:Cosmic Radiation / Q000009:adverse effects; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D006801:Humans; D018499:Linear Energy Transfer; D016273:Occupational Exposure; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000235:genetics; D011839:Radiation, Ionizing; D011874:Radiometry / Q000379:methods*; D012062:Relative Biological Effectiveness; D013026:Space Flight; D018531:Spacecraft / Q000295:instrumentation; D013997:Time Factors
chemical_list: D004247:DNA