Effect of a herbal protein, CI-1, isolated from Cajanus indicus on immune response of control and stressed mice.
The effect of a herbal protein, CI-1, purified from the leaves of Cajanus indicus was evaluated as a probable immunomodulator on immune response in control and stressed mice. In mice, sensitized with sheep red blood cells (SRBC), CI-1 enhanced IgG level by 28% over control (P < 0.05). Primary and secondary antibody response to SRBC and bovine serum albumin (BSA) were significantly increased in CI-1 treated group. Furthermore CI-1 facilitated the delayed type of hypersensitivity (DTH) response to SRBC in sensitized mice and also enhanced leucocyte and macrophage migration inhibition response in immunized mice. A fewer mice displayed symptoms of anaphylactic shock after CI-1 administration at a dose of 6.0 mg/kg body wt. in BSA sensitized mice. The immediate effect of CI-1 on anaphylactic shock was not seen when 150 microg of CI-1 was injected in combination with BSA in the shocking injection. These results suggest that CI-1 influences both humoral and cell-mediated immune response.
Datta
S
S
Department of Chemistry, Bose Institute, Calcutta, India.
Sinha
S
S
Bhattacharyya
P
P
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000707:Anaphylaxis / Q000517:prevention & control; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D010940:Plant Proteins / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D010940:Plant Proteins