title: The expression of Ca2+/calmodulin-dependent protein kinase I in rat retina is regulated by light stimulation.
abstract: Ca2+/calmodulin-dependent protein kinase I (CaM-kinase I) in rat retina was analyzed by immunohistochemical analysis, Western blot analysis and kinase activity assay. Western blot analysis revealed two immunoreactive bands similar to those detected in the brain. Developmental studies revealed that CaM-kinase I expression increased in accordance with postnatal development. Expression of CaM-kinase I in the retinas of rats raised in the complete darkness markedly decreased. CaM-kinase I activity assay supported these findings. Synapsin I was shown to be a possible intrinsic substrate of CaM-kinase I in rat retina. These results elucidated that CaM-kinase I is expressed in the retina and may play an important role in the retinal functions and that the expression of CaM-kinase I is regulated by light stimulation.
authors.affiliation: Department of Ophthalmology, Kagawa Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsumura
authors.forename: A
authors.initials: A
authors.lastname: Murata
authors.forename: F
authors.initials: F
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Sugimoto
authors.forename: E
authors.initials: E
authors.lastname: Hasegawa
authors.forename: O
authors.initials: O
authors.lastname: Hatase
authors.forename: A C
authors.initials: AC
authors.lastname: Nairn
authors.forename: M
authors.initials: M
authors.lastname: Tokuda
mesh_terms: D000818:Animals; D015153:Blotting, Western; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000032:analysis / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010775:Photic Stimulation; D011233:Precipitin Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000201:enzymology* / Q000254:growth & development; D016704:Synapsins / Q000378:metabolism
chemical_list: D016704:Synapsins; D054729:Calcium-Calmodulin-Dependent Protein Kinase Type 1; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517599:Camk1 protein, rat; C517682:Pnck protein, rat