mRNA localization: motile RNA, asymmetric anchors.
Techniques to label mRNA with green fluorescent protein (GFP) have provided the first real-time images of RNA motility in live yeast cells. Genetic screens for factors responsible for mRNA asymmetry (e. g. SHE genes) in yeast identified type V myosin among other proteins. Analysis of mRNA movement in various she mutants revealed the role of motor proteins in long-range transport, factors for particle formation, and cortical anchors for docking the mRNA.
Bloom
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K
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3280, USA. Kerry_Bloom@unc.edu
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D003599:Cytoskeleton / Q000502:physiology; D005656:Fungal Proteins / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism*
D005656:Fungal Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins