The specific endoribonuclease activity of small nuclear and cytoplasmic alpha-RNPs.
For the first time small nuclear ribonucleoprotein particles (alpha-RNP) tightly bound to chromatin as well as cytoplasmic alpha-RNP are shown to possess strong and regulated endonuclease activity specific for mRNAs and hnRNAs. The enzymatic nature of this activity is confirmed, and the optimal conditions detected. This RNase activity is controlled by the action of a differentiating stimulus, dimethylsulfoxide, in human K562 cells. Small alpha-RNP involvement in the coordinated control of stability of pre-messenger RNA and messenger RNA molecules is suggested.
Konstantinova
I M
IM
Institute of Cytology, Russian Academy of Sciences, St.-Petersburg. ikonst@link.cytspb.rssi.ru
Kulichkova
V A
VA
Evteeva
I N
IN
Mittenberg
A G
AG
Volkova
I V
IV
Ermolaeva
J B
JB
Gause
L N
LN
D020087:Alu Elements; D000818:Animals; D015152:Blotting, Northern; D004722:Endoribonucleases / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells; D008099:Liver / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D012332:RNA, Heterogeneous Nuclear / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020736:Ribonucleoproteins, Small Cytoplasmic / Q000235:genetics / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics / Q000378:metabolism*
D012332:RNA, Heterogeneous Nuclear; D012333:RNA, Messenger; D020736:Ribonucleoproteins, Small Cytoplasmic; D017411:Ribonucleoproteins, Small Nuclear; D004722:Endoribonucleases