APOBEC-1 dependent cytidine to uridine editing of apolipoprotein B RNA in yeast.
Cytidine to uridine editing of apolipoprotein B (apoB) mRNA requires the cytidine deaminase APOBEC-1 as well as a tripartite sequence motif flanking a target cytidine in apoB mRNA and an undefined number of auxiliary proteins that mediate RNA recognition and determine site-specific editing. Yeast engineered to express APOBEC-1 and apoB mRNA supported editing under conditions of late log phase growth and stationary phase. The cis -acting sequence requirements and the intracellular distribution of APOBEC-1 in yeast were similar to those described in mammalian cells. These findings suggest that auxiliary protein functions necessary for the assembly of editing complexes, or 'editosomes', are expressed in yeast and that the distribution of editing activity is to the cell nucleus.
Dance
G S
GS
Department of Biochemistry, University of Rochester, NY 14642, USA.
Sowden
M P
MP
Yang
Y
Y
Smith
H C
HC
D000071479:APOBEC-1 Deaminase; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D001483:Base Sequence; D003562:Cytidine / Q000235:genetics*; D003564:Cytidine Deaminase / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D017393:RNA Editing; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism; D014529:Uridine / Q000235:genetics*; D000098830:AICDA (Activation-Induced Cytidine Deaminase)
D001055:Apolipoproteins B; D017931:DNA Primers; D003562:Cytidine; D000098830:AICDA (Activation-Induced Cytidine Deaminase); D000071479:APOBEC-1 Deaminase; C000605091:Apobec1 protein, rat; D003564:Cytidine Deaminase; D014529:Uridine