Mucosal competitive exclusion to reduce Salmonella in swine.
A mucosal competitive exclusion culture has been shown to reduce or eliminate Salmonella spp. in poultry. Using similar techniques, a mucosal competitive exclusion culture from swine (MCES) was produced from the cecum of a 6-week-old pig. Suckling pigs were inoculated with 5 ml of MCES by oral gavage within 6 h postfarrowing (PF) and again at 24 h PE All pigs were challenged with 10(3) CFU of Salmonella Choleraesuis at 48 h PF by intranasal instillation, including pigs from two sows that had not been given MCES. Clinical signs and rectal swabs were monitored daily, and pigs were allowed to suckle throughout the experiment. All pigs underwent necropsy on day 7 PF, and presence of Salmonella was determined in both qualitative (10 tissues) and quantitative (two tissues) samples. Clinical signs were inapparent in all pigs throughout the experiment. Recovery of Salmonella from rectal swabs was variable. However, 28% of the gut tissues were positive from the MCES-treated pigs versus 79% from the control pigs. A 2- to 5-log10 reduction of Salmonella in the cecal contents or ileocolic junction was observed in the MCES-treated pigs when compared with the controls. These data indicate that use of MCES may be a useful approach for control of Salmonella.
Fedorka-Cray
P J
PJ
U.S. Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010, USA. pcray@ars.usda.gov
Bailey
J S
JS
Stern
N J
NJ
Cox
N A
NA
Ladely
S R
SR
Musgrove
M
M
D000818:Animals; D000833:Animals, Suckling; D000898:Antibiosis; D002432:Cecum / Q000382:microbiology*; D015169:Colony Count, Microbial; D004064:Digestive System / Q000382:microbiology; D005260:Female; D007413:Intestinal Mucosa / Q000382:microbiology*; D012475:Salmonella / Q000254:growth & development* / Q000302:isolation & purification; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000517:prevention & control*