title: Comparative cytogenetic study of spindle cell and pleomorphic leiomyosarcomas of soft tissues: a report from the CHAMP Study Group.
abstract: Leiomyosarcomas (LMS) of soft tissues frequently show complex karyotypic changes, and no specific aberration has been identified. The aim of this study was to search for recurrent chromosome aberrations in soft tissue LMSs and to correlate these, if present, with morphological and clinical parameters. From a series of soft tissue sarcomas thoroughly reexamined cytogenetically and histopathologically, 45 LMSs were retrieved; 35 were classified microscopically as spindle cell, 3 as epithelioid, and 7 as pleomorphic. Clonal chromosome changes were present in 14, 3, and 3 cases, respectively. This series was combined with 11 previously published, karyotypically abnormal pleomorphic LMSs for cytogenetic-clinico-histopathological correlations. The breakpoints were widely scattered, with no predilection of any of the recurrent breakpoints and losses to any of the morphologic subtypes. Combining numerical and unbalanced structural changes, the most frequently lost segments were 3p21-p23 (11 cases), 8p21-pter, 13q12-q13, 13q32-qter (10 cases each), 1q42-qter, 2p15-pter, 18p11 (9 cases each), 1p36, 11q23-qter (8 cases each), and 10q23-qter (7 cases). The most frequent gain was 1q12-q31 (6 cases). There was a greater frequency of losses in 1p and 8p and a lower frequency of losses in 10q and 13q in tumors that had metastasized than in localized tumors. We conclude that LMSs with clonal abnormalities display highly complex karyotypic changes and extensive heterogeneity. No significant correlation exists between these changes and age and sex of the patients, or with depth of tumor, topography, microscopic subtype, or tumor grade. Losses in 1p36 and 8p21-pter may be associated with increased risk of metastases. Comparison of our findings in soft tissue LMS with those previously reported in LMS in other locations suggest that the karyotypic profile is more dependent on site of origin than on microscopic features.
authors.affiliation: Department of Clinical Genetics, University Hospital, Lund, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Mandahl
authors.forename: C D
authors.initials: CD
authors.lastname: Fletcher
authors.forename: P
authors.initials: P
authors.lastname: Dal Cin
authors.forename: I
authors.initials: I
authors.lastname: De Wever
authors.forename: F
authors.initials: F
authors.lastname: Mertens
authors.forename: F
authors.initials: F
authors.lastname: Mitelman
authors.forename: J
authors.initials: J
authors.lastname: Rosai
authors.forename: A
authors.initials: A
authors.lastname: Rydholm
authors.forename: R
authors.initials: R
authors.lastname: Sciot
authors.forename: G
authors.initials: G
authors.lastname: Tallini
authors.forename: H
authors.initials: H
authors.lastname: Van Den Berghe
authors.forename: R
authors.initials: R
authors.lastname: Vanni
authors.forename: H
authors.initials: H
authors.lastname: Willén
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D019457:Chromosome Breakage; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007621:Karyotyping; D007890:Leiomyosarcoma / Q000145:classification / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D011003:Ploidies