[Preparation of 32P-labelled 2-methylthioadenosine di- and triphosphates].
2-Methylthioadenosine 5'-mono- and diphosphates were prepared by chemical synthesis, and enzymatically phosphorylated to 5'-[beta-32P]-diphosphate and 5'-[gamma-32P]-triphosphate, respectively. They were isolated by HPLC and had activity exceeding 1000 Ci/mmol and radiochemical purity higher than 95%.
Skoblov
M Iu
MIu
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Ias'ko
M V
MV
Skoblov
Iu S
IuS
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011854:Radiochemistry; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification
D010761:Phosphorus Radioisotopes; D013873:Thionucleotides; C002480:methylthio-ADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C008501:2-methylthio-ATP