title: Differentiation of phagocytic monocytes into lymph node dendritic cells in vivo.
abstract: We investigated the differentiation and trafficking of inflammatory monocytes that phagocytosed subcutaneously injected fluorescent microspheres. As expected, most of the monocytes became microsphere+ macrophages, which remained in subcutaneous tissue. However, about 25% of latex+ cells migrated to the T cell area of draining lymph nodes, where they expressed dendritic cell (DC)-restricted markers and high levels of costimulatory molecules. Microsphere-transporting cells were distinct from resident skin DCs, and this transport was reduced by more than 85% in monocyte-deficient osteopetrotic mice. Thus, a substantial minority of inflammatory monocytes carry phagocytosed particles to lymph nodes and differentiate into DCs.
authors.affiliation: Department of Pathology, Weill Medical College of Cornell University, New York, USA. gjrandol@mail.med.cornell.edu
authors.forename: G J
authors.initials: GJ
authors.lastname: Randolph
authors.forename: K
authors.initials: K
authors.lastname: Inaba
authors.forename: D F
authors.initials: DF
authors.lastname: Robbiani
authors.forename: R M
authors.initials: RM
authors.lastname: Steinman
authors.forename: W A
authors.initials: WA
authors.lastname: Muller
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology*; D005260:Female; D016130:Immunophenotyping; D007801:Langerhans Cells / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D009000:Monocytes / Q000145:classification / Q000166:cytology*; D010587:Phagocytosis; D012867:Skin / Q000166:cytology