Sequence analysis of the gene encoding the major outer membrane protein rOmp B of Rickettsia japonica.
The complete nucleotide sequence of the gene encoding rOmp B (ompB), one of the two major outer membrane antigen proteins, of Rickettsia japonica was determined and compared to those of the other spotted fever group and typhus group rickettsiae. Open reading frame of the ompB gene of R. japonica consisted of 4,968 nucleotides coding for a putative precursor protein with 1,656 amino acids (aa) in which the N-terminal 1,363 aa and the C-terminal 293 aa encode 135-kilodalton rOmp B and 32-kilodalton beta-peptide, respectively. Putative promoter and terminator sequences for transcription were present in the upstream region of the ATG start codon and downstream of the TAA stop codon. Overall sequences of the ompB genes were well conserved beyond the group, especially in the beta-peptide regions.
Uchiyama
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Department of Virology, School of Medicine, The University of Tokushima, Japan. uchiyama@basic.med.tokushima-.u.ac.jp
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012281:Rickettsia / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; C073150:rOmp B protein, Rickettsia