title: Overexpression of the RNA polymerase alpha subunit reduces transcription of Bvg-activated virulence genes in Bordetella pertussis.
abstract: Overexpression of the RNA polymerase alpha subunit in Bordetella pertussis reduces expression of the virulence factor pertussis toxin. Here we show that this reduction is at the level of transcription, is reversed by overexpression of the transcriptional activator BvgA, and is dependent on the C-terminal domain of alpha.
authors.affiliation: Department of Microbiology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA. ncarbone@umaryland.edu
authors.forename: N H
authors.initials: NH
authors.lastname: Carbonetti
authors.forename: A
authors.initials: A
authors.lastname: Romashko
authors.forename: T J
authors.initials: TJ
authors.lastname: Irish
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001886:Bordetella pertussis / Q000235:genetics* / Q000472:pathogenicity*; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D029968:Escherichia coli Proteins; C489314:EvgA protein, E coli; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit