Macrosialin may not be involved with the regulation of nitric oxide production from mouse peritoneal macrophages stimulated with LPS.
We reported previously that nitric oxide (NO) production from mouse peritoneal macrophages stimulated with LPS was inhibited by negatively charged liposomes composed of phosphatidylserine (PS-liposomes) or oxidized LDL (Ox-LDL) which have an affinity for scavenger receptors (SRs). In this study, to clarify whether macrosialin, an SR, participates in the inhibitory effects of PS-liposomes or Ox-LDL on NO production, the effects of anti-macrosialin monoclonal antibody (FA/11) on inhibition of NO production by PS-liposomes or Ox-LDL were investigated. FA/11 itself showed no effect on NO production induced by LPS. Binding of FA/11 to macrophages was inhibited by the addition of PS-liposomes or Ox-LDL, indicating that PS-liposomes or Ox-LDL have an affinity for macrosialin. However, the inhibitory effects of these ligands on NO production were not reduced in the presence of FA/11. These findings suggest that macrosialin does not contribute to the inhibitory effects of PS-liposomes or Ox-LDL on NO production from mouse peritoneal macrophages stimulated with LPS.
Aramaki
Y
Y
School of Pharmacy, Tokyo University of Pharmacy and Life Science Hachioji.
Matsuno
R
R
Arima
H
H
Tsuchiya
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008081:Liposomes; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009569:Nitric Oxide / Q000096:biosynthesis*; D010718:Phosphatidylserines / Q000494:pharmacology; D000097382:CD68 Molecule
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D008081:Liposomes; D009569:Nitric Oxide; D010718:Phosphatidylserines; C081026:CD68 protein, mouse; D000097382:CD68 Molecule; C099252:oxidized low density lipoprotein