Comparison of intraocular treatment of DMTU and SOD following retinal ischemia in rats.
The effect of intravitreal injections of DMTU (dimethylthiourea) and SOD (superoxide dismutase), two free radical scavengers, was evaluated in a rat model of retinal ischemia induced by elevated intraocular pressure. The drugs were administered just before or just after a 60 min ischemia. At days 2 and 7 after reperfusion, retinal recovery was evaluated by electroretinography. At day 7, layer thicknesses and cell rows were measured from histologic sections of paraffin-embedded retinas. In the vehicle-treated control group, we observed a decrease in the inner retinal layers and b-wave amplitude impairment. SOD injection (6 units/eye) protected the retina from ischemia/reperfusion injury. At day 2 after reperfusion, electroretinographic recovery was more efficient when SOD was administered just after ischemia (99%) than after pretreatment with SOD (81%) (p<0.03). In the DMTU-treated group (75 microg/eye), only the pretreatment induced significant electrophysiologic (40%) (p<0.001) and morphologic recovery.
Rios
L
L
Laboratoire de Biophysique Sensorielle, Faculté de Pharmacie, Université d'Auvergne, Clermont-Ferrand, France.
Cluzel
J
J
Vennat
J C
JC
Menerath
J M
JM
Doly
M
M
D000287:Administration, Topical; D000818:Animals; D004596:Electroretinography; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007511:Ischemia / Q000188:drug therapy*; D008297:Male; D009798:Ocular Hypertension / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012160:Retina / Q000187:drug effects*; D012164:Retinal Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000627:therapeutic use; D013997:Time Factors
D016166:Free Radical Scavengers; C038983:1,3-dimethylthiourea; D013482:Superoxide Dismutase; D013890:Thiourea