Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA.
In this study, the gene encoding Babesia equi merozoite antigen-2 was inserted into a baculovirus transfer vector, and a recombinant virus expressing B. equi merozoite antigen-2 was isolated. Two B. equi merozoite antigen-2-related recombinant baculovirus-expressed peptides of 25 and 30 kDa were detected with a murine anti serum against B. equi merozoite antigen-2; these corresponded to the native B. equi merozoite antigen-2 and were secreted into the supernatants of insect cell cultures. Recombinant B. equi merozoite antigen-2 was not effected by tunicamycin treatment, indicating that B. equi merozoite antigen-2 was not glycosylated. The potential of recombinant B. equi merozoite antigen-2 for use in the ELISA was evaluated by measuring the antibody response to B. equi merozoite antigen-2 in horses experimentally infected with B. equi.
Tanaka
T
T
The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Xuan
X
X
Ikadai
H
H
Igarashi
I
I
Nagasawa
H
H
Fujisaki
K
K
Mikami
T
T
Suzuki
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D001403:Babesia / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001404:Babesiosis / Q000175:diagnosis; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006734:Horse Diseases / Q000175:diagnosis; D006736:Horses; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D017422:Sequence Analysis, DNA; D018411:Spodoptera
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C111468:EMA-2 protein, Babesia equi; D015800:Protozoan Proteins; D011994:Recombinant Proteins