title: Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid.
abstract: We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line AC1-1. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, AC1-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and its underlying genetic mechanisms.
authors.affiliation: Department of Anatomy, Technical University of Aachen, Germany.
authors.forename: H G
authors.initials: HG
authors.lastname: Frank
authors.forename: B
authors.initials: B
authors.lastname: Gunawan
authors.forename: I
authors.initials: I
authors.lastname: Ebeling-Stark
authors.forename: H J
authors.initials: HJ
authors.lastname: Schulten
authors.forename: H
authors.initials: H
authors.lastname: Funayama
authors.forename: U
authors.initials: U
authors.lastname: Cremer
authors.forename: B
authors.initials: B
authors.lastname: Huppertz
authors.forename: G
authors.initials: G
authors.lastname: Gaus
authors.forename: P
authors.initials: P
authors.lastname: Kaufmann
authors.forename: L
authors.initials: L
authors.lastname: Füzesi
mesh_terms: D002459:Cell Fusion; D002822:Choriocarcinoma / Q000235:genetics*; D002871:Chromosome Banding; D016172:DNA Fingerprinting; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D011247:Pregnancy; D014327:Trophoblasts; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000235:genetics*