title: Spatial and temporal regulation of protein expression by bldA within a Streptomyces lividans colony.
abstract: The bldA gene encodes the only tRNA for the UUA codon that, although dispensable in genes important for primary vegetative growth of Streptomyces spp., is important in genes that serve a regulatory purpose in the differentiation. To investigate this role further, the spatial and temporal expression profiles of the bldA-regulated and unregulated genes within a Streptomyces colony were examined using modified genes for the green fluorescent protein (gfp) as an expression-tag. A comparative study, based on computer-assisted quantitative analysis of the GFP fluorescence, revealed that the presence of TTA codons in gfp results in a temporal delay of translation and, consequently, changed the spatial pattern of the GFP expression within a colony, especially during early differentiation. The delay of GFP expression was undetectable at 60 h post-inoculation. These results provide the first extensive evidence that the bldA does indeed play a significant regulatory role during colony differentiation.
authors.affiliation: Project Research Center, Mitsubishikasei Institute of Life Sciences, Machida, Tokyo, Japan. mars@libra.ls.m-kagaku.co.jp
authors.forename: M
authors.initials: M
authors.lastname: Kataoka
authors.forename: S
authors.initials: S
authors.lastname: Kosono
authors.forename: G
authors.initials: G
authors.lastname: Tsujimoto
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010957:Plasmids / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D012356:RNA, Transfer, Leu / Q000096:biosynthesis*; D013302:Streptomyces / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; C107344:BldA tRNA, Streptomyces coelicolor; D008164:Luminescent Proteins; D012329:RNA, Bacterial; D012356:RNA, Transfer, Leu; D049452:Green Fluorescent Proteins