title: Comparison of intraocular treatment of DMTU and SOD following retinal ischemia in rats.
abstract: The effect of intravitreal injections of DMTU (dimethylthiourea) and SOD (superoxide dismutase), two free radical scavengers, was evaluated in a rat model of retinal ischemia induced by elevated intraocular pressure. The drugs were administered just before or just after a 60 min ischemia. At days 2 and 7 after reperfusion, retinal recovery was evaluated by electroretinography. At day 7, layer thicknesses and cell rows were measured from histologic sections of paraffin-embedded retinas. In the vehicle-treated control group, we observed a decrease in the inner retinal layers and b-wave amplitude impairment. SOD injection (6 units/eye) protected the retina from ischemia/reperfusion injury. At day 2 after reperfusion, electroretinographic recovery was more efficient when SOD was administered just after ischemia (99%) than after pretreatment with SOD (81%) (p<0.03). In the DMTU-treated group (75 microg/eye), only the pretreatment induced significant electrophysiologic (40%) (p<0.001) and morphologic recovery.
authors.affiliation: Laboratoire de Biophysique Sensorielle, Faculté de Pharmacie, Université d'Auvergne, Clermont-Ferrand, France.
authors.forename: L
authors.initials: L
authors.lastname: Rios
authors.forename: J
authors.initials: J
authors.lastname: Cluzel
authors.forename: J C
authors.initials: JC
authors.lastname: Vennat
authors.forename: J M
authors.initials: JM
authors.lastname: Menerath
authors.forename: M
authors.initials: M
authors.lastname: Doly
mesh_terms: D000287:Administration, Topical; D000818:Animals; D004596:Electroretinography; D016166:Free Radical Scavengers / Q000627:therapeutic use*; D007511:Ischemia / Q000188:drug therapy*; D008297:Male; D009798:Ocular Hypertension / Q000209:etiology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012160:Retina / Q000187:drug effects*; D012164:Retinal Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D013482:Superoxide Dismutase / Q000627:therapeutic use*; D013890:Thiourea / Q000031:analogs & derivatives* / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D016166:Free Radical Scavengers; C038983:1,3-dimethylthiourea; D013482:Superoxide Dismutase; D013890:Thiourea