Prediction of rodent carcinogenicity utilizing a battery of in vitro and in vivo genotoxicity tests.
The primary purpose of this study is to investigate the degree to which we can improve the prediction of rodent carcinogenicity (CA) by combining results from an in vitro and two in vivo genotoxicity tests. We used the Ames Salmonella assay (SAL) for the in vitro test and the micronucleus assay (MNC) and chromosome aberration assay (ABS) in mouse bone marrow cells for the two in vivo tests. We collected complete assay data for 82 chemicals (55 carcinogens and 27 noncarcinogens) from the NTP data base and the IARC monograph series. Our results indicate that: (1) only SAL affects the predictivity of CA, (2) MNC has a strong association with ABS, and (3) SAL predicts ABS. It has been known for some time that once the SAL assay result is available for prediction, other in vitro mutation tests provide little additional information for predicting CA. Our study indicates that the same conclusion holds for CA, SAL, MNC, and ABS.
Kim
B S
BS
Department of Applied Statistics, Yonsei University, Seoul, South Korea. bskim@yonsei.ac.kr
Margolin
B H
BH
D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D002273:Carcinogens / Q000633:toxicity; D002869:Chromosome Aberrations; D016208:Databases, Factual; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests / Q000379:methods*; D011237:Predictive Value of Tests; D012377:Rodentia; D012475:Salmonella / Q000187:drug effects / Q000235:genetics
D002273:Carcinogens