title: Recruitment and activation of natural killer (NK) cells in vivo determined by the target cell phenotype. An adaptive component of NK cell-mediated responses.
abstract: Natural killer (NK) cells can spontaneously lyse certain virally infected and transformed cells. However, early in immune responses NK cells are further activated and recruited to tissue sites where they perform effector functions. This process is dependent on cytokines, but it is unclear if it is regulated by NK cell recognition of susceptible target cells. We show here that infiltration of activated NK cells into the peritoneal cavity in response to tumor cells is controlled by the tumor major histocompatibility complex (MHC) class I phenotype. Tumor cells lacking appropriate MHC class I expression induced NK cell infiltration, cytotoxic activation, and induction of transcription of interferon gamma in NK cells. The induction of these responses was inhibited by restoration of tumor cell MHC class I expression. The NK cells responding to MHC class I-deficient tumor cells were approximately 10 times as active as endogenous NK cells on a per cell basis. Although these effector cells showed a typical NK specificity in that they preferentially killed MHC class I-deficient cells, this specificity was even more distinct during induction of the intraperitoneal response. Observations are discussed in relation to a possible adaptive component of the NK response, i.e., recruitment/activation in response to challenges that only NK cells are able to neutralize.
authors.affiliation: Microbiology Center, Karolinska Institute, S-171 77 Stockholm, Sweden. rickard.glas@mtc.ki.se
authors.forename: R
authors.initials: R
authors.lastname: Glas
authors.forename: L
authors.initials: L
authors.lastname: Franksson
authors.forename: C
authors.initials: C
authors.lastname: Une
authors.forename: M L
authors.initials: ML
authors.lastname: Eloranta
authors.forename: C
authors.initials: C
authors.lastname: Ohlén
authors.forename: A
authors.initials: A
authors.lastname: Orn
authors.forename: K
authors.initials: K
authors.lastname: Kärre
mesh_terms: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000502:physiology; D000818:Animals; D000941:Antigens / Q000032:analysis; D000954:Antigens, Surface; D002460:Cell Line; D015395:Histocompatibility Antigens Class I / Q000502:physiology*; D007371:Interferon-gamma / Q000235:genetics; D018664:Interleukin-12 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010641:Phenotype; D011506:Proteins / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D000941:Antigens; D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; C489516:Tap2 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma