[Effect of short-term culture of blood lymphocytes in vitro on the ability of Fcgamma-receptors to bind IgG1 and IgG2 and mediation of antibody-dependent cytotoxicity].
The comparative study incubation influence at 37 degrees C on the lymphocyte Fc gamma-receptor (Fc gamma R) binding ability to IgG1 and IgG2 and on the antibody-dependent cell mediated cytotoxicity (ADCC) was performed. By means of the fluorometric binding assay it was determined that during 30 min of incubation the binding ability of Fc gamma R decreased while after 180 min of incubation it increased. Upon comparing both IgG1 and IgG2 binding abilities to lymphocytes no statistically important changes were noticed (p < 0.05). The changes in the binding ability of Fc gamma R for IgG1 and IgG2 had a similar pattern to the changes.
Miliukene
V V
VV
Biochemical Institute of Lithuanian Republic, Vilnius.
Dedinene
Ia L
IaL
D000920:Antibody-Dependent Cell Cytotoxicity; D018929:Cell Culture Techniques; D005470:Fluorometry; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D011485:Protein Binding; D017452:Receptors, IgG / Q000378:metabolism*
D007074:Immunoglobulin G; D017452:Receptors, IgG