Hypoosmolarity influences the activity of transcription factor NF-kappaB in rat H4IIE hepatoma cells.
The influence of anisoosmolarity on NF-kappaB binding activity was studied in H4IIE rat hepatoma cells. Hypoosmolarity induced a sustained NF-kappaB binding activity whereas the hyperosmotic NF-kappaB response was only minor. Hypoosmotic NF-kappaB activation was accompanied by degradation of the inhibitory IkappaB-alpha. Protein kinase C, PI(3)-kinase, reactive oxygen intermediates and the proteasome apparently participate in mediating the hypoosmotic effect on NF-kappaB. Hypoosmolarity plus PMA induced, amplified and prolonged IkappaB-alpha degradation and NF-kappaB binding activity. Transforming growth factor beta-induced apoptosis was diminished by hypoosmolarity. However, this anti-apoptotic effect was probably not related to NF-kappaB activation.
Michalke
M
M
Department of Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University, Moorenstr. 5, D-40225, D]usseldorf, Germany.
Cariers
A
A
Schliess
F
F
Häussinger
D
D
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008113:Liver Neoplasms; D016328:NF-kappa B / Q000737:chemistry / Q000378:metabolism*; D009994:Osmolar Concentration; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011065:Poly(ADP-ribose) Polymerases; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D013755:Tetradecanoylphorbol Acetate; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D004791:Enzyme Inhibitors; D016328:NF-kappa B; D000081082:Phosphoinositide-3 Kinase Inhibitors; D016212:Transforming Growth Factor beta; D011065:Poly(ADP-ribose) Polymerases; D011493:Protein Kinase C; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases; D013755:Tetradecanoylphorbol Acetate