Expression profile and chromosomal location of cDNA clones, identified from an enriched adult retina library.
OBJECTIVE
To delineate the profile of genes expressed in the adult human retina and assign chromosomal location of cDNA clones.


METHODS
The end-sequence of random clones from an enriched human retinal cDNA library was analyzed by NCBI database search. Expression profile was established by northern blot analysis, database search, or both. Selected cDNA clones were localized to human chromosomes by somatic cell hybrid analysis, in situ hybridization to metaphase chromosomes, or both. Chromosomal location was also obtained by searching the databases.


RESULTS
One hundred and thirty-seven clones were isolated from the subtracted retinal library. Fifty-one clones were identical with 35 known human genes in GenBank, and 24 clones corresponded to 23 uncharacterized human expressed sequenced tags (ESTs), novel genes, or both. The remaining 59 clones were not pursued further because they contained bacterial sequences or repetitive elements. Several clones indicated a restricted pattern of expression with high levels of transcripts in the retina. Chromosomal location of novel retinal ESTs is also reported.


CONCLUSIONS
This study provides a profile of genes expressed in the adult human retina. One round of subtraction eliminated most constitutively expressed genes and permitted partial normalization of the retinal library. Twenty-three novel genes were identified. The combined information obtained from expression analysis and chromosomal localization of retinal cDNAs should be valuable in identifying candidate genes for diseases involving retinal dysfunction.
Sinha
S
S
Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.
Sharma
A
A
Agarwal
N
N
Swaroop
A
A
Yang-Feng
T L
TL
D015152:Blotting, Northern; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis*; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D012160:Retina / Q000378:metabolism*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D005136:Eye Proteins