title: MRI of thermally denatured blood: methemoglobin formation and relaxation effects.
abstract: Focal regions of T1-shortening have been observed in magnetic resonance imaging (MRI)-monitored thermal ablations of perfused tissues. The aims of this study were two-fold: to find evidence for heat-induced conversion of hemoglobin (Hb) to methemoglobin (mHb), and to investigate the effects of heat treatment of in-vitro blood components upon their MR relaxation times. Spectrophotometric studies were performed to confirm the heat-induced formation of methemoglobin. Preparations of whole and fractionated blood, previously submitted to elevated temperatures of 40 degrees C to 80 degrees C, were imaged and the relaxation times were calculated. Optical absorption spectra of samples containing free Hb, heated to 60 degrees C, showed increased light absorption at 630 nm, evident of mHb presence. Short T1 values in whole blood (1.13 s) and packed red blood cell (0.65 s) compartments, heated at 60 degrees C, compared to their baseline values (1.62 s and 0.83 s, respectively), were attributed to mHb formation. In relation to MRI-guided thermal interventions, these results suggest a possible explanation for observation of hyperintense regions on T1-weighted images.
authors.affiliation: Department of Radiological Sciences, UCLA School of Medicine, Los Angeles, CA 90024-1721, USA. kfarahan@ucla.edu
authors.forename: K
authors.initials: K
authors.lastname: Farahani
authors.forename: R E
authors.initials: RE
authors.lastname: Saxton
authors.forename: H C
authors.initials: HC
authors.lastname: Yoon
authors.forename: A A
authors.initials: AA
authors.lastname: De Salles
authors.forename: K L
authors.initials: KL
authors.lastname: Black
authors.forename: R B
authors.initials: RB
authors.lastname: Lufkin
mesh_terms: D000818:Animals; D001769:Blood / Q000378:metabolism*; D004912:Erythrocytes / Q000737:chemistry; D006358:Hot Temperature; D066298:In Vitro Techniques; D008279:Magnetic Resonance Imaging; D008297:Male; D008706:Methemoglobin / Q000096:biosynthesis*; D010949:Plasma / Q000737:chemistry; D011489:Protein Denaturation; D013053:Spectrophotometry; D013552:Swine
chemical_list: D008706:Methemoglobin