Quantitative changes of metabolic and bioenergetic parameters in experimental tumors during fractionated irradiation.
OBJECTIVE
Previous studies with rat rhabdomyosarcomas indicate that during fractionated irradiation profound alterations of the tumor microvasculature and the oxygenation status occur when the total dose exceeds 45 Gy. At this dose a destruction which included all structures of the vessels and a significant worsening in tumor oxygenation were found. The aim of the present study was to analyze whether these effects of fractionated irradiation on the microvasculature and on tumor oxygenation also induce changes in the bioenergetic and metabolic status in the tumors during radiation treatment.


METHODS
R1H rhabdomyosarcomas of the rat implanted into the flank were irradiated with 60Co-gamma-rays using 5 fractions of 3 Gy per week over 5 weeks. During this irradiation schedule, tumors were investigated each week for the microregional distributions of glucose, lactate, and ATP concentrations. For this, tumors were rapidly excised, shock-frozen and quantitative bioluminescence measurements were performed on tumor tissue sections.


RESULTS
ATP concentrations remained unchanged during fractionated irradiation up to a total dose of 45 Gy. Above this dose, a significant decrease in ATP levels was observed. Lactate concentrations changed only slightly during irradiation whereas glucose levels increased continuously over the whole irradiation period.


CONCLUSIONS
During fractionated irradiation of R1H tumors with a total dose of 75 Gy, the bioenergetic and metabolic status of the tumors changed considerably. This became most obvious once a dose of 45 Gy had been achieved. The severe energy depletion and worsening of tumor oxygenation might be the result of destruction of tumor blood vessels as has been described previously in the same tumor model. The modification of the tumor micromilieu appears to be an important parameter in the responsiveness of tumor cells to radiation and for local tumor control.
Thews
O
O
Institute of Physiology and Pathophysiology, University of Mainz, Germany. OLTHEWS@mail.uni-mainz.de
Zywietz
F
F
Lecher
B
B
Vaupel
P
P
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D003037:Cobalt Radioisotopes; D019583:Dose Fractionation, Radiation; D005947:Glucose / Q000378:metabolism / Q000528:radiation effects*; D019344:Lactic Acid / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D009368:Neoplasm Transplantation; D011853:Radiobiology; D051381:Rats; D012208:Rhabdomyosarcoma / Q000378:metabolism* / Q000532:radiotherapy; D013997:Time Factors
D003037:Cobalt Radioisotopes; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D005947:Glucose