title: Macrosialin may not be involved with the regulation of nitric oxide production from mouse peritoneal macrophages stimulated with LPS.
abstract: We reported previously that nitric oxide (NO) production from mouse peritoneal macrophages stimulated with LPS was inhibited by negatively charged liposomes composed of phosphatidylserine (PS-liposomes) or oxidized LDL (Ox-LDL) which have an affinity for scavenger receptors (SRs). In this study, to clarify whether macrosialin, an SR, participates in the inhibitory effects of PS-liposomes or Ox-LDL on NO production, the effects of anti-macrosialin monoclonal antibody (FA/11) on inhibition of NO production by PS-liposomes or Ox-LDL were investigated. FA/11 itself showed no effect on NO production induced by LPS. Binding of FA/11 to macrophages was inhibited by the addition of PS-liposomes or Ox-LDL, indicating that PS-liposomes or Ox-LDL have an affinity for macrosialin. However, the inhibitory effects of these ligands on NO production were not reduced in the presence of FA/11. These findings suggest that macrosialin does not contribute to the inhibitory effects of PS-liposomes or Ox-LDL on NO production from mouse peritoneal macrophages stimulated with LPS.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Science Hachioji.
authors.forename: Y
authors.initials: Y
authors.lastname: Aramaki
authors.forename: R
authors.initials: R
authors.lastname: Matsuno
authors.forename: H
authors.initials: H
authors.lastname: Arima
authors.forename: S
authors.initials: S
authors.lastname: Tsuchiya
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology / Q000502:physiology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008081:Liposomes; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009569:Nitric Oxide / Q000096:biosynthesis*; D010718:Phosphatidylserines / Q000494:pharmacology; D000097382:CD68 Molecule
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D008070:Lipopolysaccharides; D008077:Lipoproteins, LDL; D008081:Liposomes; D009569:Nitric Oxide; D010718:Phosphatidylserines; C081026:CD68 protein, mouse; D000097382:CD68 Molecule; C099252:oxidized low density lipoprotein