Cross-linked dimers with nucleating activity in actin prepared from muscle acetone powder.
A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin on Western blots and migrates during reduced polyacrylamide gel electrophoresis like actin cross-linked with N, N'-p-phenylenebismaleimide. The origin of the cross-linked dimer appears to be related to the presence of carbonyl groups in purified actin. A large number of carbonyls (approximately 0.3/actin) are introduced into actin during the prolonged treatment with acetone in the preparation of the muscle acetone powder from which actin is extracted. Actin extracted from acetone powder prepared by a single acetone wash and actin prepared from bovine spleen, which is not washed with acetone, both contain fewer carbonyl groups (approximately 0.05 carbonyl/actin). ANF forms spontaneously in solutions of polymer actin containing 0.3 carbonyl/actin. We speculate that a reaction between a carbonyl on one actin polymer subunit and a lysine on a neighboring subunit is responsible for ANF formation. The presence of cross-linked actin dimers in commonly used skeletal muscle actin preparations could certainly affect studies of actin polymerization and, particularly, studies of the nucleation reaction. The physiological relevance of ANF is not clear, but given the large cellular concentration of actin, similar reactions yielding ANF could occur in vivo when increased levels of reactive oxygen species are present.
Selden
L A
LA
Research Service, Stratton VA Medical Center, Albany, New York 12208, USA.
Kinosian
H J
HJ
Estes
J E
JE
Gershman
L C
LC
D000096:Acetone; D000199:Actins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D011208:Powders; D011817:Rabbits; D013154:Spleen
D000199:Actins; D003432:Cross-Linking Reagents; D011108:Polymers; D011208:Powders; D000096:Acetone; D002244:Carbon