Mitotic regulators govern progress through steps in the centrosome duplication cycle.
Centrosome duplication is marked by discrete changes in centriole structure that occur in lockstep with cell cycle transitions. We show that mitotic regulators govern steps in centriole replication in Drosophila embryos. Cdc25(string), the expression of which initiates mitosis, is required for completion of daughter centriole assembly. Cdc20(fizzy), which is required for the metaphase-anaphase transition, is required for timely disengagement of mother and daughter centrioles. Stabilization of mitotic cyclins, which prevents exit from mitosis, blocks assembly of new daughter centrioles. Common regulation of the nuclear and centrosome cycles by mitotic regulators may ensure precise duplication of the centrosome.
Vidwans
S J
SJ
Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California-San Francisco, San Francisco, California 94143-0448, USA.
Wong
M L
ML
O'Farrell
P H
PH
D000595:Amino Acid Sequence; D000818:Animals; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D002502:Centrioles / Q000235:genetics / Q000502:physiology; D018385:Centrosome / Q000502:physiology*; D016213:Cyclins / Q000502:physiology; D004330:Drosophila; D029721:Drosophila Proteins; D008938:Mitosis / Q000502:physiology*; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000502:physiology; D017027:Protein Tyrosine Phosphatases; D029701:Saccharomyces cerevisiae Proteins; D020687:cdc25 Phosphatases / Q000502:physiology
C577495:CDC20 protein, S cerevisiae; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D016213:Cyclins; D029721:Drosophila Proteins; D029701:Saccharomyces cerevisiae Proteins; C577502:fzy protein, Drosophila; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; D020687:cdc25 Phosphatases; C059217:stg protein, Drosophila