Serum proteins by capillary zone electrophoresis: approaches to the definition of reference values.
The Paragon CZE 2000 (Beckman Analytical, Milan, Italy) is an automatic dedicated capillary zone electrophoresis (CZE) system, producing a five-zone serum protein pattern with quantitative estimation of the zones. With the view of substituting this instrument for two previously used serum protein electrophoresis techniques, we planned to produce reference values for the "new" systems leading to compatible interpretation of the results. High resolution cellulose acetate electrophoresis with visual inspection and descriptive reporting (HR-CAE) and five-zone cellulose acetate electrophoresis with densitometry (CAE-D) were the previously used techniques. Serum samples (n = 167) giving "normal pattern" with HR-CAE were assayed with the CZE system, and the results were statistically assessed to yield 0.95 reference intervals. One thousand normal and pathological serum samples were then assayed with the CAE-D and the CZE techniques, and the regression equations of the CAE-D values over the CZE values for the five zones were used to transform the CAE-D reference limits into the CZE reference limits. The two sets of reference values thereby produced were in good agreement with each other and also with reference values previously reported for the CZE system. Thus, reference values for the CZE techniques permit interpretation of results coherent with the previously used techniques and reporting modes.
Petrini
C
C
Laboratorio di Biochimica, Ospedale San Carlo, Milano, Italy.
Alessio
M G
MG
Scapellato
L
L
Brambilla
S
S
Franzini
C
C
D001798:Blood Proteins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006801:Humans; D012015:Reference Standards
D001798:Blood Proteins