Methods for quantitation of human neutrophil proteins, a survey.
Neutrophils contain a variety of proteins that endow the cell with its capacity to migrate towards and eliminate microbial pathogens. Many of these proteins are largely or exclusively localized to neutrophils. It is therefore of interest to quantitate these proteins in a variety of clinical settings as well as in basic research. The aim of this survey is to give an introduction to some of the more commonly used methods for quantitation of neutrophil proteins and to discuss advantages and problems of the different methods and the relevance of quantitating neutrophil proteins in different biological settings.
Sørensen
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O
The Granulocyte Research Laboratory, Department of Hematology L-9322, The Finsen Centre, The National University Hospital, Rigshospitalet, 9 Blegdamsvej, DK-2100, Copenhagen, Denmark. olesoeren@rh.dk
Borregaard
N
N
D006801:Humans; D007118:Immunoassay; D007150:Immunohistochemistry; D009504:Neutrophils / Q000737:chemistry* / Q000201:enzymology* / Q000276:immunology; D011506:Proteins / Q000737:chemistry*
D011506:Proteins