Chiral separation of beta-blockers after derivatization with (-)-menthyl chloroformate by reversed-phase high performance liquid chromatography.
Optimum conditions of chiral derivatization reaction of beta-blockers acebutolol, arotinolol, beta-xolol, bisoprolol, celiprolol, metoprolol and pindolol) with (-)-menthyl chloroformate were investigated for the resolution by HPLC. With more than 30 times molar excess of (-)-menthyl chloroformate chiral derivatization reactions were completed within one hour at room temperature except arotinolol and celiprolol. Diastereomeric derivatives of beta-blockers were well resolved on the ODS column using acetonitrile-methanol-water as a mobile phase.
Kim
K H
KH
College of Pharmacy, Kangwon National University, Chunchon, Korea. kyeong@cc.kangwon.ac.kr
Choi
P W
PW
Hong
S P
SP
Kim
H J
HJ
D000097:Acetonitriles / Q000737:chemistry; D000319:Adrenergic beta-Antagonists / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005561:Formates / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D013237:Stereoisomerism; D013997:Time Factors; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D000319:Adrenergic beta-Antagonists; D005561:Formates; D014867:Water; C059663:menthyl chloroformate; D000432:Methanol; C032159:acetonitrile