title: Toxicokinetics of DDE and 2-chlorobiphenyl in Chironomus tentans.
abstract: Uptake, biotransformation, and elimination rates for DDE and 2-chlorobiphenyl (2-CB) were examined using fourth instar midge larvae (Chironomus tentans) under a water-only exposure. A two-compartment model that included biotransformation described the kinetics for 2-CB and a one-compartment model was used for DDE. The uptake clearance coefficient (k(u)) was 65.96 +/- 2.09 ml x g(-1) midge x h(-1) for 2-CB and 84.1 +/- 2.7 ml x g(-1) midge x h(-1) for DDE. DDE demonstrated no measurable elimination, while 2-CB showed elimination with faster elimination of parent compound (k(ep) = 0.100 +/- 0.008 h(-1)) compared to the polar metabolites (k(em) = 0.073 +/- 0.016 h(-1)). The biotransformation rate for 2-CB (k(m) = 0.031 +/- 0.005 h(-1)) appeared to be time-dependent with faster rates at the beginning of the accumulation process slowing to a constant once the midge was loaded with chemical. Experimental results indicate a difference in the ability of C. tentans to eliminate 2-CB and its metabolites more quickly than DDE, and these differences were related to the bioconcentration potential of the compounds.
authors.affiliation: Department of Biological Sciences, 1845 Fairmount, Wichita State University, Wichita, Kansas 67260-0026, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lydy
authors.forename: J L
authors.initials: JL
authors.lastname: Lasater
authors.forename: P F
authors.initials: PF
authors.lastname: Landrum
mesh_terms: D000818:Animals; D001711:Biotransformation; D001713:Biphenyl Compounds / Q000493:pharmacokinetics / Q000633:toxicity; D002683:Chironomidae / Q000187:drug effects*; D003633:Dichlorodiphenyl Dichloroethylene / Q000493:pharmacokinetics / Q000633:toxicity*; D007306:Insecticides / Q000493:pharmacokinetics / Q000633:toxicity*; D007814:Larva / Q000187:drug effects
chemical_list: D001713:Biphenyl Compounds; D007306:Insecticides; C055142:2-chlorobiphenyl; D003633:Dichlorodiphenyl Dichloroethylene