Role of polyamines in mediating malignant transformation and oncogene expression.
Previous studies have demonstrated that polyamines accumulate in cancer cells and that overproduction of ornithine decarboxylase (ODC), which catalyzes polyamine synthesis, elicits the acquisition of the transformed phenotype. However, it was not clear whether the expression of ODC and the accumulation of polyamines are only innocent by-products of the transformation process. In this study we confirm previous findings what polyamines can trigger the transformation of immortalized cultured cells. In addition to NIH 3T3 fibroblasts, studied previously, rat kidney epithelial cells or fibroblasts also grew in soft agar in the presence of polyamines. It has also been demonstrated that spermidine, preferentially stimulated the transcription and the expression of c-myc while those of c-fos were preferentially stimulated by putrescine. These findings suggest that the effect of polyamines on cellular transformation, could be explained, at least partially, by stimulation of proto-oncogene expression.
Tabib
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Department of Molecular Biology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Bachrach
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U
D016475:3T3 Cells; D000818:Animals; D015317:Biogenic Polyamines / Q000494:pharmacology* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos; D016259:Genes, myc; D051379:Mice; D011519:Proto-Oncogenes; D011700:Putrescine / Q000494:pharmacology / Q000502:physiology; D051381:Rats; D013095:Spermidine / Q000494:pharmacology / Q000502:physiology
D015317:Biogenic Polyamines; D013095:Spermidine; D011700:Putrescine