title: Interleukin-8 stimulates placental prostacyclin production in preeclampsia.
abstract: OBJECTIVE
  Our purpose was to determine placental interleukin (IL)-8 production and its correlation with the prostacyclin production in normal and preeclamptic pregnancies and to evaluate the beneficial effect of IL-8 on prostacyclin production.
  
  
  METHODS
  We determined 1) the in vitro production of IL-8 and prostacyclin by placental villous tissues from normal and preeclamptic pregnancies and 2) the production of prostacyclin by villous tissues from preeclampsia treated with recombinant human IL-8 (rhIL-8). IL-8 levels were measured by enzyme-linked immunosorbent assay and prostacyclin by radioimmunoassay of 6-keto PGF1alpha, the stable metabolite of prostacyclin.
  
  
  RESULTS
  1) Placental production of IL-8 and 6-keto PGF1alpha were significantly less in preeclampsia than in normal pregnancies, P<0.05. 2) Placental production of 6-keto PGF1alpha and IL-8 was significantly correlated in preeclampsia, P<0.01. 3) Placental tissues treated with IL-8 exhibited a concentration-dependent increase in 6-keto PGF1alpha production.
  
  
  CONCLUSIONS
  Placental tissues from preeclampsia produce significantly less IL-8 than tissues from normal pregnancies, which correlates with decreased prostacyclin production. IL-8 improves placental prostacyclin production in preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Louisiana State University Medical Center, Shreveport 71130, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Baier
authors.forename: C D
authors.initials: CD
authors.lastname: Adair
authors.forename: D F
authors.initials: DF
authors.lastname: Lewis
authors.forename: S
authors.initials: S
authors.lastname: Krueger
authors.forename: T
authors.initials: T
authors.lastname: Kruger
authors.forename: M
authors.initials: M
authors.lastname: Gurski
authors.forename: E
authors.initials: E
authors.lastname: Brown
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000328:Adult; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D016209:Interleukin-8; D015121:6-Ketoprostaglandin F1 alpha