title: Ethanol-induced apoptosis in vitro.
abstract: OBJECTIVE
  The aim is to study the apoptotic process in a human hepatocyte model for ethanol (EtOH)-induced apoptosis.
  
  
  METHODS
  Normal human primary hepatocytes (HPH) and Hep G2 cells were exposed to increasing EtOH. 6000 cells/ sample were analyzed by transmission electron microscopy.
  
  
  RESULTS
  Apoptotic cells were observed (mmol/L EtOH): 40: 6 +/-0.5%, 60:13 +/- 2% (p < 0.05), 80: 26 +/- 1% (p < 0.001) (vs. control). Two consecutive doses of 80 mmol/L for 24 h each additionally increased apoptosis 55 +/- 3% (p < 0.0001 vs. control and p < 0.001 vs. single dose). In response to this exposure, there is a stronger apoptotic activity in HPH when compared to Hep G2 (p < 0.05).
  
  
  CONCLUSIONS
  In vitro, EtOH-induced apoptosis is regulated by dose level and the frequency of exposure.
authors.affiliation: Division of Clinical Pharmacology, Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario, Canada. manuela@srcl.sunnybrook.utoronto.ca
authors.forename: M G
authors.initials: MG
authors.lastname: Neuman
authors.forename: N H
authors.initials: NH
authors.lastname: Shear
authors.forename: R G
authors.initials: RG
authors.lastname: Cameron
authors.forename: G
authors.initials: G
authors.lastname: Katz
authors.forename: C
authors.initials: C
authors.lastname: Tiribelli
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron
chemical_list: D000431:Ethanol