Microglia exhibit clonal variability in eliciting cytotoxic T lymphocyte responses independent of class I expression.
Microglia are important immunoregulatory cells within the central nervous system (CNS). Viral infection of primary microglia and splenic macrophage clones revealed that both exhibited a heterogeneous, but relatively low, sensitivity to cytolysis mediated by CD8(+) cytotoxic T lymphocytes (CTL). The majority of clones were poor in processing and presenting epitopes, despite triggering lysis when coated with peptide. These characteristics were retained by stable microglia lines. Reduced lysis did not correlate with class I expression and IFN-gamma treatment only partially enhanced recognition. In contrast, targeting the epitope into the endoplasmic reticulum restored cytolysis to levels achieved with exogenous peptide. An inherent resistance to cytolysis was revealed by efficient engagement of T cells in competition assays and the inability of saturating peptide to enhance cytolysis. These data suggest that microglia heterogeneity in antigen processing, in addition to low sensitivity to CTL lysis, contributes to limited CD8(+) T cell responses and viral CNS persistence.
Bergmann
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CC
Department of Neurology, University of Southern California Keck School of Medicine, Los Angeles 90033, USA.
Yao
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Q
Stohlman
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SA
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D017628:Microglia / Q000276:immunology* / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism*
D006183:H-2 Antigens