Molecular cloning and characterization of the Arabidopsis thaliana alpha-subunit of elongation factor 1B.
Using a PCR-based approach, we have isolated two Arabidopsis thaliana cDNA clones (alpha1 and alpha2) encoding the alpha-subunit of translation elongation factor 1B (eEF1Balpha). They encode open reading frames of 228 and 224 amino acids respectively, with extensive homology to eEF1Balpha subunits from different organisms, particularly in the C-terminal half of the protein. They both lack a conserved phosphorylation site that has been implicated in regulating nucleotide exchange activity. Using a plasmid shuffling experiment, we demonstrated that both alpha1 and alpha2 clones are able to complement a mutant yeast strain deficient for the eEF1Balpha subunit. This provides evidence that Arabidopsis encodes at least two functional isoforms of this subunit, termed eEF1Balpha1 and eEF1Balpha2. A third cDNA clone was isolated that appeared to result from an alternative splicing event of the eEF1Balpha1 gene.
Héricourt
F
F
Laboratoire de Virologie Moléculaire, Institut Jacques Monod, UMR 7592, CNRS-Universités Paris 6 & Paris 7, 2 place Jussieu, 75251, Paris, France.
Jupin
I
I
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D020648:Peptide Elongation Factor 1