Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with nic96p.
A fraction of the yeast nucleoporin Nic96p is localized at the terminal ring of the nuclear basket. When Nic96p was affinity purified from glutaraldehyde-treated spheroplasts, it was found to be associated with Mlp2p. Mlp2p, together with Mlp1p, are the yeast Tpr homologues, which form the nuclear pore-attached intranuclear filaments (Strambio-de-Castillia, C., Blobel, G., and Rout, M. P. (1999) J. Cell Biol. 144, 839-855). Double disruption mutants of MLP1 and MLP2 are viable and apparently not impaired in nucleocytoplasmic transport. However, overproduction of MLP1 causes nuclear accumulation of poly(A)(+) RNA in a chromatin-free area of the nucleus.
Kosova
B
B
BZH, Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
Panté
N
N
Rollenhagen
C
C
Podtelejnikov
A
A
Mann
M
M
Aebi
U
U
Hurt
E
E
D000595:Amino Acid Sequence; D001692:Biological Transport; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005976:Glutaral; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D009685:Nuclear Envelope / Q000737:chemistry / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013104:Spheroplasts; D016707:Tissue Fixation; D014466:Ultraviolet Rays / Q000009:adverse effects; D015003:Yeasts / Q000378:metabolism / Q000528:radiation effects; D028901:alpha Karyopherins; D028961:beta Karyopherins
D005656:Fungal Proteins; C080988:MLP1 protein, S cerevisiae; C119200:MLP2 protein, S cerevisiae; D008565:Membrane Proteins; C082533:NIC96 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D028901:alpha Karyopherins; D028961:beta Karyopherins; D005976:Glutaral