Gene expression of double-stranded RNA-dependent protein kinase in the human endometrium and decidua.
To clarify the biological significance of double-stranded RNA-dependent protein kinase (PKR), an interferon (INF)-inducible substance, we investigated (1) PKR gene expression and the (2) effect of IFN-gamma on PKR gene expression in human endometrium. By Northern blot analysis, PKR mRNA was detected as a 2.5 kb band in human endometrium throughout the menstrual cycle and decidua in early pregnancy. The addition of IFN-gamma to culture medium increased the PKR mRNA level in a dose-dependent manner in cultured endometrial stromal cells. These results suggest that IFN-gamma, which is reported to have an inhibitory effect on cell proliferation, plays an important role in human endometrial function by mediating PKR gene expression.
Taga
M
M
Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Yokohama, Japan.
Ikeda
M
M
Shigeta
H
H
Hiraga
M
M
D015152:Blotting, Northern; D002478:Cells, Cultured; D003656:Decidua / Q000166:cytology / Q000201:enzymology*; D004717:Endometrium / Q000166:cytology / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019892:eIF-2 Kinase / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D007371:Interferon-gamma; D019892:eIF-2 Kinase