title: Antagonistic signals between BMP4 and FGF8 define the expression of Pitx1 and Pitx2 in mouse tooth-forming anlage.
abstract: Members of the Pitx/RIEG family of homeodomain-containing transcription factors have been implicated in vertebrate organogenesis. In this study, we examined the expression and regulation of Pitx1 and Pitx2 during mouse tooth development. Pitx1 expression is detected in early development in a widespread pattern, in both epithelium and mesenchyme, covering the tooth-forming region in the mandible, and is then maintained in the dental epithelium from the bud stage to the late bell stage. Pitx2 expression, on the other hand, is restricted to the dental epithelium throughout odontogenesis. Interestingly, from E9.5 to E10.5, the expression domains of Pitx1 and Pitx2, in the developing mandible, overlap with that of Fgf8 but are exclusive to the zone of Bmp4 expression. Bead implantation experiments demonstrate that ectopic expression of Fgf8 can induce/maintain the expression of both Pitx1 and Pitx2 at E9.5. In contrast, Bmp4-expressing tissues and BMP4-soaked beads were able to repress Pitx1 expression in mandibular mesenchyme and Pitx2 expression in the presumptive dental epithelium, respectively. However, the effects of FGF8 and BMP4 are transient. It thus appears that the early expression patterns of Pitx1 and Pitx2 in the developing mandible are regulated by the antagonistic effects of FGF8 and BMP4 such that the Pitx1 and Pitx2 expression patterns are defined. These results indicate that the epithelial-derived signaling molecules are responsible not only for restricting specific gene expression in the dental mesenchyme, but also for defining gene expression in the dental epithelium.
authors.affiliation: Department of Cell Biology, Tulane University, New Orleans, Louisiana, 70118, USA.
authors.forename: T R
authors.initials: TR
authors.lastname: St Amand
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: E V
authors.initials: EV
authors.lastname: Semina
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: L
authors.initials: L
authors.lastname: Nguyen
authors.forename: J C
authors.initials: JC
authors.lastname: Murray
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D051957:MSX1 Transcription Factor; D008334:Mandible / Q000196:embryology; D051379:Mice; D009154:Mutation; D009687:Nuclear Proteins; D009805:Odontogenesis; D051761:Paired Box Transcription Factors; D015398:Signal Transduction; D014018:Tissue Distribution; D014070:Tooth / Q000196:embryology; D014083:Tooth Germ / Q000196:embryology; D014157:Transcription Factors / Q000096:biosynthesis*; D000097577:Homeobox Protein PITX2
chemical_list: D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D004268:DNA-Binding Proteins; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors; D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; D009687:Nuclear Proteins; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; D000097577:Homeobox Protein PITX2; C525810:Bmp4 protein, mouse; C496457:Fgf8 protein, mouse; C084119:MSX2 protein; C101485:homeobox protein PITX1; C433629:homeobox protein PITX3