Different induction of gulonolactone oxidase in aromatic hydrocarbon-responsive or -unresponsive mouse strains.
The role of aromatic hydrocarbon receptor (AhR)-mediated signal transduction pathways was investigated in the regulation of ascorbate synthesis by using Ah-responsive and Ah-unresponsive mouse strains. In vivo 3-methylcholanthrene treatment increased hepatic and plasma ascorbate concentrations only in the Ah-responsive strain. The mRNA level of gulonolactone oxidase and the microsomal ascorbate production from p-nitrophenyl glucuronide, D-glucuronic acid or gulonolactone in the liver of Ah-responsive and Ah-unresponsive mice were compared. In Ah-responsive mice, these parameters were higher originally, and they further increased upon in vivo addition of 3-methylcholanthrene, while in Ah-unresponsive mice the treatment was not effective. These results suggest that the transcription of gulonolactone oxidase gene is regulated by an Ah receptor-dependent signal transduction pathway.
Braun
L
L
Department of Medical Chemistry, Semmelweis University of Medicine, H-1444, Budapest, Hungary.
Kardon
T
T
El Koulali
K
K
Csala
M
M
Mandl
J
J
Bánhegyi
G
G
D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis; D005937:Glucaric Acid / Q000031:analogs & derivatives; D005965:Glucuronates; D020723:Glucuronic Acid / Q000378:metabolism; D020719:Glucuronides / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000096:biosynthesis; D050544:L-Gulonolactone Oxidase; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008862:Microsomes, Liver / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D018336:Receptors, Aryl Hydrocarbon / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction; D013401:Sugar Alcohol Dehydrogenases / Q000096:biosynthesis*; D013997:Time Factors
D005965:Glucuronates; D020719:Glucuronides; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; C002730:4-nitrophenylglucuronide; D008748:Methylcholanthrene; C009194:saccharolactone; D020723:Glucuronic Acid; D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D019363:Cytochrome P-450 CYP1A1; C110655:UDP-glucuronosyltransferase, UGT1A6; D014453:Glucuronosyltransferase; D001205:Ascorbic Acid; D005937:Glucaric Acid