title: Beryllofluoride mimics phosphorylation of NtrC and other bacterial response regulators.
abstract: Two-component systems, sensor kinase-response regulator pairs, dominate bacterial signal transduction. Regulation is exerted by phosphorylation of an Asp in receiver domains of response regulators. Lability of the acyl phosphate linkage has limited structure determination for the active, phosphorylated forms of receiver domains. As assessed by both functional and structural criteria, beryllofluoride yields an excellent analogue of aspartyl phosphate in response regulator NtrC, a bacterial enhancer-binding protein. Beryllofluoride also appears to activate the chemotaxis, sporulation, osmosensing, and nitrate/nitrite response regulators CheY, Spo0F, OmpR, and NarL, respectively. NMR spectroscopic studies indicate that beryllofluoride will facilitate both biochemical and structural characterization of the active forms of receiver domains.
authors.affiliation: Department of Plant Biology, University of California, Berkeley, CA 94720, USA. dyan@nature.berkeley.edu
authors.forename: D
authors.initials: D
authors.lastname: Yan
authors.forename: H S
authors.initials: HS
authors.lastname: Cho
authors.forename: C A
authors.initials: CA
authors.lastname: Hastings
authors.forename: M M
authors.initials: MM
authors.lastname: Igo
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: J G
authors.initials: JG
authors.lastname: Pelton
authors.forename: V
authors.initials: V
authors.lastname: Stewart
authors.forename: D E
authors.initials: DE
authors.lastname: Wemmer
authors.forename: S
authors.initials: S
authors.lastname: Kustu
mesh_terms: D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D001426:Bacterial Proteins / Q000378:metabolism*; D001608:Beryllium / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005459:Fluorides / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins; D019906:Nuclear Magnetic Resonance, Biomolecular; D051922:PII Nitrogen Regulatory Proteins; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D015398:Signal Transduction; D015534:Trans-Activators; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D051922:PII Nitrogen Regulatory Proteins; D010750:Phosphoproteins; D015534:Trans-Activators; D014157:Transcription Factors; C066821:FliM protein, Bacteria; C032197:beta-aspartyl phosphate; D001224:Aspartic Acid; C035982:beryllium fluoride; D001608:Beryllium; D005459:Fluorides