title: Regional pulmonary blood flow during partial liquid ventilation in normal and acute oleic acid-induced lung-injured piglets.
abstract: OBJECTIVE
  To determine the spatial distribution of pulmonary blood flow in three groups of piglets: partial liquid ventilation in normal piglets, partial liquid ventilation during acute lung injury, and conventional gas ventilation during acute lung injury.
  
  
  METHODS
  Prospective randomized study.
  
  
  METHODS
  A university medical school laboratory approved for animal research.
  
  
  METHODS
  Neonatal piglets.
  
  
  METHODS
  Regional pulmonary blood flow was studied in 21 piglets in the supine position randomized to three different groups: a normal group that received partial liquid ventilation (Normal-PLV) and two acute lung injury groups that received an oleic acid-induced lung injury: partial liquid ventilation during acute lung injury (OA-PLV) and conventional gas ventilation during acute lung injury (OA-Control). Acute lung injury was induced by infusing oleic acid (0.15 mL/kg iv) over 30 mins. Partial liquid ventilation was instituted with perflubron (LiquiVent, 30 mL/kg) after 30 mins in the Normal-PLV and OA-PLV groups.
  
  
  RESULTS
  Arterial and venous blood gases, hemodynamics, and pulmonary mechanics were measured every 15 mins throughout the hour-long study. Pulmonary blood flow was assessed by fluorescent microsphere technique at baseline and after 30, 45, and 60 mins. In the Normal-PLV piglets, pulmonary blood flow decreased from baseline (before injury or partial liquid ventilation) in the most dependent areas of the lung (F ratio = 3.227; p < .001). In the OA-PLV piglets, pulmonary blood flow was preserved over time throughout the lung (F ratio = 1.079; p = .38). In the OA-Control piglets, pulmonary blood flow decreased in the most dependent areas of the lung and increased from baseline in less dependent slices over time (F ratio = 2.48; p = .003).
  
  
  CONCLUSIONS
  The spatial distribution of regional pulmonary blood flow is preserved during partial liquid ventilation compared with gas ventilation in oleic acid-induced lung injury.
authors.affiliation: Division of Pediatric Critical Care Medicine, University of California at Davis Medical Center, Sacramento, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Enrione
authors.forename: M C
authors.initials: MC
authors.lastname: Papo
authors.forename: C L
authors.initials: CL
authors.lastname: Leach
authors.forename: B A
authors.initials: BA
authors.lastname: Holm
authors.forename: L J
authors.initials: LJ
authors.lastname: Hernan
authors.forename: B P
authors.initials: BP
authors.lastname: Fuhrman
authors.forename: M S
authors.initials: MS
authors.lastname: Dowhy
authors.forename: M G
authors.initials: MG
authors.lastname: Rath
authors.forename: P E
authors.initials: PE
authors.lastname: Frisicaro
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006439:Hemodynamics; D008171:Lung Diseases / Q000139:chemically induced / Q000503:physiopathology* / Q000628:therapy; D008863:Microspheres; D019301:Oleic Acid; D011652:Pulmonary Circulation; D011659:Pulmonary Gas Exchange; D012121:Respiration, Artificial / Q000379:methods*; D013552:Swine
chemical_list: D019301:Oleic Acid