Evaluation of neutrophil structure and function by electron microscopy: cytochemical studies.
Methods for studying human neutrophils at the ultrastructural level by enzyme cytochemistry and immunocytochemistry are presented. The focus of these methods is on the analysis of the alkaline phosphatase-positive secretory organelle of these cells. These methods provide unique information which, when coupled with biochemical studies, provide for the most complete analysis of neutrophil structure and function.
Robinson
J M
JM
Department of Physiology and Cell Biology, Ohio State University, 302 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210, USA. robinson.21@osu.edu
Kobayashi
T
T
Seguchi
H
H
Takizawa
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007399:Interphase / Q000276:immunology; D008854:Microscopy, Electron / Q000379:methods*; D016253:Microscopy, Immunoelectron; D018375:Neutrophil Activation / Q000276:immunology; D009504:Neutrophils / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D010100:Oxygen / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D013481:Superoxides; D000469:Alkaline Phosphatase; D010100:Oxygen