Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database.
We propose a prediction method for human full-length cDNA by comparing sequence data between human genome shotgun sequence and mouse full-length cDNA. The human genome which is homologous to the mouse full-length cDNA is selected by a homology search program, and the predicted exons are connected at the exon-intron junction which gives the best homology score to the mouse full-length cDNA. The accuracy of the predicted human full-length coding region is 83.3%, and the false positive rate is 16.7%. Five human full-length proteins out of 20 proteins are correctly predicted.
Fukunishi
Y
Y
Genome Science Laboratory, Tsukuba Lifescience Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki, Japan. fukunisi@rtc.riken.go.jp
Suzuki
H
H
Yoshino
M
M
Konno
H
H
Hayashizaki
Y
Y
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D016208:Databases, Factual; D005091:Exons; D006801:Humans; D051379:Mice; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary