Mycoplasma gallisepticum -induced release of macrophage inflammatory protein-1 beta from chicken monocytes-macrophages.
Chicken monocytes and a macrophage-like cell line were used to determine the presence of macrophage inflammatory protein-1 beta (MIP-1 beta). RNA was extracted from these cells and subjected to reverse transcription with an anti-sense primer specific for the whole length of the MIP-1 beta cDNA. After a polymerase chain reaction to amplify the cDNA, a 200 bp gene product was detected, which corresponded to the molecular weight of the MIP-1 beta. The culture supernate of these cells did not have the ability to attract heterophils. However, after infection with Mycoplasma gallisepticum, a potent chemotactic substance in the culture supernate was detected. The chemotactic activity could be neutralized by antibodies prepared against two 10-amino-acid peptides of MIP-1 beta, indicating that the substance was MIP-1 beta.
Lam
K M
KM
Department of Population Health and Reproduction, School of Veterinary Medicine, Davis, CA 95616, USA.
DaMassa
A J
AJ
D000818:Animals; D002461:Cell Line, Transformed; D054407:Chemokine CCL4; D002630:Chemotactic Factors / Q000378:metabolism; D002645:Chickens / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000032:analysis; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D009000:Monocytes / Q000378:metabolism* / Q000382:microbiology; D009174:Mycoplasma / Q000502:physiology*; D009500:Neutralization Tests; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054407:Chemokine CCL4; D002630:Chemotactic Factors; D017931:DNA Primers; D018076:DNA, Complementary; D019402:Macrophage Inflammatory Proteins; D012313:RNA