title: CD4 + /CD7- T cell frequency and polymerase chain reaction-based clonality assay correlate with stage in cutaneous T cell lymphomas.
abstract: In cutaneous T cell lymphomas, tumor cells can be found in skin and in other compartments. A precise definition of extracutaneous spread including blood involvement is necessary for staging and treatment design. We investigated peripheral blood in 51 patients with various types of cutaneous T cell lymphomas by the analysis of blood smears for Sézary cells, the CD4 + /CD7- T helper cell frequency in the peripheral blood by fluorescence activated cell sorter analysis and by polymerase chain reaction for the T cell receptor gamma-chain followed by denaturing gradient gel electrophoresis. Eleven polymerase chain reaction products were sequenced. Thirty-five per cent of patients with stage Ia-IIb cutaneous T cell lymphomas presented a peripheral blood T cell clone. In patients with stage III-IVb cutaneous T cell lymphomas 75% were positive for clonality in the peripheral blood by polymerase chain reaction. Interestingly, three of 13 Sézary patients showed a TCR-gamma joining region pseudogene (JgammaP1/JgammaP2) usage. CD4 + /CD7- cell counts were significantly higher in patients with advanced cutaneous T cell lymphomas than in patients with early cutaneous T cell lymphomas. There was a correlation between increased percentage of circulating CD4 + /CD7- cells and detection of clonality by polymerase chain reaction (p = 0.001). There was no significant correlation between the polymerase chain reaction data and the percentage of Sézary cells on blood smears. A significant correlation between CD4 + /CD7- cells and Sézary cells was found, however. Stepwise logistic regression analysis showed that the CD4 + /CD7- cell count and clonal T cell detection in peripheral blood are independently correlated with stage. The combination of both parameters gives more information than each one separately. In conclusion, our data indicate that fluorescence activated cell sorter analysis of peripheral blood and polymerase chain reaction-based clonality assays can improve the accuracy of staging investigations in cutaneous T cell lymphomas patients.
authors.affiliation: Department of Dermatology, University Hospital, Zurich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Laetsch
authors.forename: A C
authors.initials: AC
authors.lastname: Häffner
authors.forename: U
authors.initials: U
authors.lastname: Döbbeling
authors.forename: B
authors.initials: B
authors.lastname: Seifert
authors.forename: E
authors.initials: E
authors.lastname: Ludwig
authors.forename: G
authors.initials: G
authors.lastname: Burg
authors.forename: R
authors.initials: R
authors.lastname: Dummer
mesh_terms: D019011:Antigens, CD7 / Q000032:analysis*; D001773:Blood Cells / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology*; D002452:Cell Count; D002469:Cell Separation; D002999:Clone Cells; D004586:Electrophoresis; D005434:Flow Cytometry; D006801:Humans; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000473:pathology*; D009000:Monocytes / Q000473:pathology; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction / Q000379:methods; D012044:Regression Analysis; D012867:Skin / Q000473:pathology; D012878:Skin Neoplasms / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology*
chemical_list: D019011:Antigens, CD7