title: Inhibition of experimental autoimmune uveoretinitis with anti-macrophage migration inhibitory factor antibodies.
abstract: OBJECTIVE
  The role of macrophage migration inhibitory factor (MIF) in the regulation of ocular autoimmune disease was studied in experimental autoimmune uveoretinitis (EAU) in rats following immunization with a retinal antigen (Ag), interphotoreceptor retinoid-binding protein (IRBP).
  
  
  METHODS
  LEW rats were immunized with a single injection of IRBP derived peptide, R16(ADGSSWEGVGVVPDV). A neutralizing monoclonal antibody (mAb, IgM) to MIF was injected intraperitoneally every second day from day 0 to day 6 (group A), or from day 8 to day 14 (group B). Control rats were treated with unrelated mouse IgM or PBS. T cell proliferative responses were measured 12 days after immunization. The occurrence and severity of EAU were observed and compared among experimental and control groups.
  
  
  RESULTS
  T cell proliferative responses against R16 were inhibited in rats treated with anti-MIF mAb compared with the control rats. The development of EAU was delayed in the rats of group A in comparison with those of group B and the control group. The mean histological EAU score on day 18 in group A was 1.11 +/- 0. 11 and significantly lower than those of the group B (1.29 +/- 0.19) and the control (1.67 +/- 0.19).
  
  
  CONCLUSIONS
  The present result suggests that MIF plays an important role in induction of EAU.
authors.affiliation: Department of Ophthalmology, School of Medicine, Institute of Immunological Science, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kitaichi
authors.forename: A
authors.initials: A
authors.lastname: Matsuda
authors.forename: S
authors.initials: S
authors.lastname: Kotake
authors.forename: K
authors.initials: K
authors.lastname: Namba
authors.forename: Y
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Sasamoto
authors.forename: K
authors.initials: K
authors.lastname: Ogasawara
authors.forename: K
authors.initials: K
authors.lastname: Iwabuchi
authors.forename: K
authors.initials: K
authors.lastname: Onoé
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
authors.forename: J
authors.initials: J
authors.lastname: Nishihira
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D001327:Autoimmune Diseases / Q000139:chemically induced / Q000517:prevention & control*; D005123:Eye / Q000187:drug effects / Q000473:pathology; D005136:Eye Proteins; D007274:Injections, Intraperitoneal; D008263:Macrophage Migration-Inhibitory Factors / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D011917:Rats, Inbred Lew; D012173:Retinitis / Q000139:chemically induced / Q000517:prevention & control*; D012177:Retinol-Binding Proteins / Q000737:chemistry; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014605:Uveitis / Q000139:chemically induced / Q000517:prevention & control*; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D000911:Antibodies, Monoclonal; D005136:Eye Proteins; D008263:Macrophage Migration-Inhibitory Factors; D010446:Peptide Fragments; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial