Impact of sample type on rapid detection of influenza virus A by cytospin-enhanced immunofluorescence and membrane enzyme-linked immunosorbent assay.
Cytospin-enhanced direct fluorescent-antibody assay (DFA) detected 49 (92.5%) and rapid membrane enzyme-linked immunosorbent assay (ELISA) detected 40 (75.5%) of 53 influenza virus A-positive samples. All 15 positive nasopharyngeal aspirates from children were detected by both tests. In contrast, 34 of 38 (89.5%) positive swabs from adults were detected by DFA, but only 25 (66%) were detected by ELISA.
Landry
M L
ML
Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 06520, USA. marie.landry@yale.edu
Cohen
S
S
Ferguson
D
D
D000328:Adult; D000367:Age Factors; D002498:Centrifugation; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis*; D008168:Lung / Q000821:virology; D009305:Nasopharynx / Q000821:virology; D013048:Specimen Handling