Effects of toluene on regulation of adenylyl cyclase by stimulation of G-protein-coupled receptors expressed in CHO cells.
We determined the effects of toluene exposure on activation or inhibition of adenylyl cyclase by stimulating human beta2-adrenergic receptors (beta2-AR) and muscarinic acetylcholine receptor (mAChR) m2 subtypes, respectively, expressed in CHO cells. The formation of cAMP via beta2-AR stimulation was slightly but not significantly facilitated in the presence of 3.7 microM toluene. On the other hand, the inhibition of adenylyl cyclase by 10 microM of carbamylcholine stimulation of mAChR m2 subtypes was attenuated in the presence of toluene. These results strongly suggest that toluene affects activation of Gi rather than Gs.
Tsuga
H
H
Division of Health Effects Research, National Institute of Industrial Health, Kawasaki, Kanagawa, Japan.
Wang
R S
RS
Honma
T
T
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002217:Carbachol / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D000242:Cyclic AMP / Q000096:biosynthesis / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D043585:Receptor, Muscarinic M2; D018343:Receptors, Adrenergic, beta-2 / Q000187:drug effects* / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000378:metabolism; D014050:Toluene / Q000494:pharmacology*
D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D043585:Receptor, Muscarinic M2; D018343:Receptors, Adrenergic, beta-2; D011976:Receptors, Muscarinic; D005576:Colforsin; D014050:Toluene; D002217:Carbachol; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases