Distribution of JC virus DNA in peripheral blood lymphocytes of hematological disease cases.
OBJECTIVE
The distribution of JC virus DNA in peripheral blood was surveyed by the polymerase chain reaction using the late genes as markers.


RESULTS
Six out of 52 cases of hematological diseases and one systemic lupus erythematosus case out of 17 cases were positive for JCV DNA. After separation into B and T lymphocytes by a cell sorter, JCV DNA was found in both cell types prepared from adult T cell leukemia and PML patients.


CONCLUSIONS
Only 1 or 2 copies of JCV genome were calculated to exist in a cell based on the time course analysis of PCR. Only B lymphocytes and glial brain cells are known to produce nuclear factors which support the growth of the virus. The result that B lymphocytes contained a copy number of JCV genome similar to T lymphocytes suggests that there is some barrier to viral growth in susceptible B lymphocytes, and that the growth of JCV is different from that of other virulent viruses.
Shimizu
N
N
Second Department of Internal Medicine, Aichi Medical University, Nagakute, Aichi-gun.
Imamura
A
A
Daimaru
O
O
Mihara
H
H
Kato
Y
Y
Kato
R
R
Oguri
T
T
Fukada
M
M
Yokochi
T
T
Yoshikawa
K
K
Komatsu
H
H
Ueda
R
R
Nitta
M
M
D000368:Aged; D000369:Aged, 80 and over; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006402:Hematologic Diseases / Q000097:blood* / Q000821:virology*; D006801:Humans; D007577:JC Virus / Q000235:genetics*; D008214:Lymphocytes / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D004279:DNA, Viral