Changes of free fatty acids and acyl-CoAs in rat brain hippocampal slice with tetraethylammonium-induced long-term potentiation.
We investigated the role of acyl-CoAs during induction and maintenance of long-term potentiation in rat brain hippocampus. Changes of acyl-CoA and free fatty acids (FFA) in hippocampus were measured during tetraethylammonium (TEA)-induced LTP. Results indicated that concentrations of acyl-CoAs and FFAs in slices were changed during TEA-induced LTP and 16:0-CoA and 18:0-CoA were increased in the early phase of stimulation, whereas free fatty acids in this phase were rather decreased. The increase of 20:4-CoA was delayed more than saturated acyl-CoAs. To examine the role of acyl-CoA in LTP of evoked transmitter release, we measured the glutamate release from hippocampal slice with the addition of acyl-CoA using glutamate electrode. Acyl-CoA (16:0-, 18:1-, and 20:4-CoA) could enhance glutamate release in hippocampal slice. It is suggested that saturated acyl-CoAs may play a functional role in the early phase of LTP.
Zhang
Q
Q
Research Laboratory Center, Oita Medical University, Hasama-cho, Oita, 879-5593, Japan.
Yoshida
S
S
Sakai
K
K
Liu
J
J
Fukunaga
K
K
D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D004558:Electric Stimulation; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D019789:Tetraethylammonium / Q000494:pharmacology*
D000214:Acyl Coenzyme A; D005230:Fatty Acids, Nonesterified; D018698:Glutamic Acid; D019789:Tetraethylammonium