A mammalian H+ channel generated through alternative splicing of the NADPH oxidase homolog NOH-1.
Voltage-gated proton (H+) channels are found in many human and animal tissues and play an important role in cellular defense against acidic stress. However, a molecular identification of these unique ion conductances has so far not been achieved. A 191-amino acid protein is described that, upon heterologous expression, has properties indistinguishable from those of native H+ channels. This protein is generated through alternative splicing of messenger RNA derived from the gene NOH-1 (NADPH oxidase homolog 1, where NADPH is the reduced form of nicotinamide adenine dinucleotide phosphate).
Bánfi
B
B
Biology of Aging Laboratory, Department of Geriatrics, Geneva University Hospitals, Geneva Medical School, CH-1211 Geneva 4, Switzerland.
Maturana
A
A
Jaconi
S
S
Arnaudeau
S
S
Laforge
T
T
Sinha
B
B
Ligeti
E
E
Demaurex
N
N
Krause
K H
KH
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D004553:Electric Conductivity; D004579:Electron Transport; D020224:Expressed Sequence Tags; D006801:Humans; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating; D007473:Ion Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D000074624:NADPH Oxidase 1; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000737:chemistry / Q000235:genetics*; D018408:Patch-Clamp Techniques; D011522:Protons; D014162:Transfection; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000494:pharmacology
D007473:Ion Channels; D008562:Membrane Glycoproteins; D011522:Protons; D006859:Hydrogen; C056558:CYBB protein, human; D000074624:NADPH Oxidase 1; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C402799:NOX1 protein, human; D015032:Zinc