title: Sp1 and AP2 transcription factors are required for the human fragile mental retardation promoter activity in SK-N-SH neuronal cells.
abstract: The promoter of the human fragile mental retardation gene (FMR1) was functionally analyzed in order to identify elements responsible for its regulation. Plasmids carrying the wild type or different deleted-promoter sequences driving the chloramphenicol acetyl transferase gene (CAT) were transiently transfected into the SK-N-SH cells and the CAT activity was assessed. Deletion studies suggested that major regulatory elements are present in a DNA region between positions -123 and -51. Gel mobility shift and footprinting assays using a DNA fragment encompassing that promoter region showed that SP1 and AP2 transcription factors could be involved in the functioning of the FMR1 promoter.
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., Mexico City, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Carrillo
authors.forename: B
authors.initials: B
authors.lastname: Cisneros
authors.forename: C
authors.initials: C
authors.lastname: Montañez
mesh_terms: D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004247:DNA / Q000235:genetics; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000502:physiology*; D051860:Fragile X Messenger Ribonucleoprotein 1; D017353:Gene Deletion; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009474:Neurons / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D016601:RNA-Binding Proteins; D016329:Sp1 Transcription Factor / Q000502:physiology*; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000502:physiology*; D014162:Transfection
chemical_list: D015500:Chloramphenicol O-Acetyltransferase; D004247:DNA; D004268:DNA-Binding Proteins; D051860:Fragile X Messenger Ribonucleoprotein 1; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; D016329:Sp1 Transcription Factor; D050656:Transcription Factor AP-2; D014157:Transcription Factors; C497683:FMR1 protein, human