title: Autoradiographic localization of tachykinin and calcitonin gene-related peptide receptors in adult urinary bladder.
abstract: OBJECTIVE
  In bladder, sensory afferent nerve fibers contain the "sensory neuropeptides" substance P (SP), neurokinin A (NKA) and calcitonin gene-related peptide (CGRP), which interact with tachykinin NK-1 and NK-2 receptors and CGRP receptors, respectively. The purpose of this study was to examine the autoradiographic distribution of these three receptor types in the human bladder, to determine whether the anatomic location of the receptors was consistent with their known functional roles.
  
  
  METHODS
  Specimens of urinary bladder from 9 patients (58-74 years) were obtained at cystectomy. Frozen sections of dome were labeled with [125I]-Bolton-Hunter [Sar9,Met(O2)11]-SP (NK-1 receptors), [125I]-[Lys5,Tyr(I2)7,MeLeu9,Nle10]-NKA(4-10) (NK-2 receptors) and [125I]-rat CGRP-I. Binding sites were visualized using emulsion autoradiography.
  
  
  RESULTS
  NK-1 receptors were found over the endothelium of arterial blood vessels within the detrusor muscle and lamina propria, and over small vessels in the subepithelium. NK-2 receptors were seen over the detrusor muscle and very sparsely over blood vessels, whereas CGRP receptors were expressed densely over the smooth muscle layer of arteries and arterioles, and weakly over collecting venules. NK-1 and CGRP receptors were not observed over the detrusor muscle.
  
  
  CONCLUSIONS
  Although the afferent nerves contain all three peptides, not all cell types express receptors for each peptide. The general distribution of receptors is in good agreement with the location of nerves, and with the known actions of SP and CGRP as vasodilator agents, and of NKA (but not SP or CGRP) in contracting the detrusor muscle.
authors.affiliation: Department of Urology, Prince Henry and Prince of Wales Hospitals, Australia.
authors.forename: E
authors.initials: E
authors.lastname: Burcher
authors.forename: X P
authors.initials: XP
authors.lastname: Zeng
authors.forename: J
authors.initials: J
authors.lastname: Strigas
authors.forename: F
authors.initials: F
authors.lastname: Shang
authors.forename: R J
authors.initials: RJ
authors.lastname: Millard
authors.forename: K H
authors.initials: KH
authors.lastname: Moore
mesh_terms: D000368:Aged; D001345:Autoradiography; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000032:analysis*; D018040:Receptors, Neurokinin-1 / Q000032:analysis*; D018041:Receptors, Neurokinin-2 / Q000032:analysis*; D001743:Urinary Bladder / Q000737:chemistry*
chemical_list: D018015:Receptors, Calcitonin Gene-Related Peptide; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2