Correlation of astroglial cell function on micro-patterned surfaces with specific geometric parameters.
Microcontact printing techniques were used to modify silicon substrates with arrays of hexagonal features of N1[3-(trimethoxysilyl) propyl]diethylenetriamine (DETA) surrounded by octadecyltrichlorosilane (OTS), which are hydrophilic, cell-adhesive and hydrophobic, non-adhesive organosilanes, respectively. In the presence of serum proteins, LRM55 cell adhesion and morphology on these modified surfaces were best correlated to the width of the cell-adhesive features. On surfaces modified with small (5 microm in width) cell-adhesive features, LRM55 cells elaborated only thin processes. As feature width was increased, cells on these surfaces exhibited increased cell spreading and elaborated wide processes. On surfaces modified with large (>35 microm in width) features, single cells adhered to and spread upon individual DETA features. In a similar fashion, LRM55 cell adhesion density increased with increasing feature width; this correlation could be represented by a simple, second-order relation, and was independent of all other measures of pattern geometry. The results of this study provide evidence that micro-patterning may be effective in controlling astrocyte interaction with implant materials.
Kam
L
L
Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA.
Shain
W
W
Turner
J N
JN
Bizios
R
R
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002448:Cell Adhesion; D002455:Cell Division; D048429:Cell Size; D007700:Kinetics; D051381:Rats; D013499:Surface Properties; D013997:Time Factors; D014407:Tumor Cells, Cultured