A transient decrease of electrochemical gradient stabilizes DNA structural change in single mitochondria of living cells.
The effect of controlled and reversible perturbation of the electrochemical gradient on the structural changes of mitochondrial DNA has been studied in living cells by fluorescence microscopy. Electrochemical gradient perturbations were induced by the protonophore carbonyl cyanide p-trifluoromethoxyphenylhydrazone and quantified by measuring the mitochondrial membrane potential using tetramethyl rhodamine methyl ester. Under our experimental conditions, we have shown that ethidium fluorescence was mainly due to ethidium molecules intercalated in mtDNA. Ethidium fluorescence variations have been used to probe DNA structural changes. This showed that: i) electrochemical gradient perturbations induced mtDNA structural change; ii) this change was readily reversible following a total but short collapse of the electrochemical gradient; iii) in contrast, a short and weak perturbation of the electrochemical gradient stabilized the mtDNA structural change; and iv) the degree of weak depolarization varied from cell to cell, showing the necessity of studying the effect of energetic perturbations at the level of an individual cell.
Durieux
C
C
Département de Recherche, Institut Curie, Paris, France.
Brunet
A C
AC
Geeraert
V
V
Coppey
J
J
Coppey-Moisan
M
M
D000818:Animals; D001278:Atractyloside / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002522:Chlorocebus aethiops; D004272:DNA, Mitochondrial / Q000737:chemistry* / Q000502:physiology*; D004563:Electrochemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004996:Ethidium; D005456:Fluorescent Dyes; D007476:Ionophores / Q000494:pharmacology; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000502:physiology*; D009690:Nucleic Acid Conformation; D009840:Oligomycins / Q000494:pharmacology; D012235:Rhodamines; D014709:Vero Cells
D004272:DNA, Mitochondrial; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007476:Ionophores; D009840:Oligomycins; D012235:Rhodamines; C401833:tetramethylrhodamine methyl ester; D001278:Atractyloside; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D004996:Ethidium