Information transfer between large and small two-dimensional polyacrylamide gel electrophoresis.
To determine the feasibility of data transfer, an interlaboratory comparison was conducted on colon carcinoma cell line (DLD-1) proteins resolved by two-dimensional polyacrylamide gel electrophoresis either on small (6 x 7 cm) or large (16x18 cm) gels. The gels were silver-stained and scanned by laser densitometry, and the image obtained was analyzed using Melanie software. The number of spots detected was 1337+/-161 vs. 2382+/-176 for small vs. large format gels, respectively. After gel calibration using landmarks determined using pl and Mr markers, large- and small-format gels were matched and 712+/-36 proteins were found on both types of gels. Having performed accurate gel matching it was possible to acquire additional information after accessing a 2-D PAGE reference database (http://www.expasy.ch/ cgibin/map2/def?DLD1_HUMAN). Thus, the difference in gel size is not an obstacle for data transfer. This will facilitate exchanges between laboratories or consultation concerning existing databases.
Felley-Bosco
E
E
Institut de Pharmacologie et de Toxicologie, Lausanne, Switzerland.
Demalte
I
I
Barcelo
S
S
Sanchez
J C
JC
Hochstrasser
D F
DF
Schlegel
W
W
Reymond
M A
MA
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D014407:Tumor Cells, Cultured