title: Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development.
abstract: Endoglin (CD105) is expressed on the surface of endothelial and haematopoietic cells in mammals and binds TGFbeta isoforms 1 and 3 in combination with the signaling complex of TGFbeta receptors types I and II. Endoglin expression increases during angiogenesis, wound healing, and inflammation, all of which are associated with TGFbeta signaling and alterations in vascular structure. The importance of endoglin for normal vascular architecture is further indicated by the association of mutations in the endoglin gene with the inherited disorder Hereditary Haemorrhagic Telangiectasia Type 1 (HHT1), a disease characterised by bleeding from vascular malformations. In order to study the role of endoglin in vivo in more detail and to work toward developing an animal model of HHT1, we have derived mice that carry a targeted nonsense mutation in the endoglin gene. Studies on these mice have revealed that endoglin is essential for early development. Embryos homozygous for the endoglin mutation fail to progress beyond 10.5 days postcoitum and fail to form mature blood vessels in the yolk sac. This phenotype is remarkably similar to that of the TGFbeta1 and the TGFbeta receptor II knockout mice, indicating that endoglin is needed in vivo for TGFbeta1 signaling during extraembryonic vascular development. In addition, we have observed cardiac defects in homozygous endoglin-deficient embryos, suggesting endoglin also plays a role in cardiogenesis. We anticipate that heterozygous mice will ultimately serve as a useful disease model for HHT1, as some individuals have dilated and fragile blood vessels similar to vascular malformations seen in HHT patients.
authors.affiliation: SMIVS, School of Biochemistry and Genetics, United Kingdom. helen.arthur@ncl.ac.uk
authors.forename: H M
authors.initials: HM
authors.lastname: Arthur
authors.forename: J
authors.initials: J
authors.lastname: Ure
authors.forename: A J
authors.initials: AJ
authors.lastname: Smith
authors.forename: G
authors.initials: G
authors.lastname: Renforth
authors.forename: D I
authors.initials: DI
authors.lastname: Wilson
authors.forename: E
authors.initials: E
authors.lastname: Torsney
authors.forename: R
authors.initials: R
authors.lastname: Charlton
authors.forename: D V
authors.initials: DV
authors.lastname: Parums
authors.forename: T
authors.initials: T
authors.lastname: Jowett
authors.forename: D A
authors.initials: DA
authors.lastname: Marchuk
authors.forename: J
authors.initials: J
authors.lastname: Burn
authors.forename: A G
authors.initials: AG
authors.lastname: Diamond
mesh_terms: D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D018388:Codon, Terminator; D017931:DNA Primers; D000071063:Endoglin; D004730:Endothelium, Vascular / Q000378:metabolism; D005804:Genes, Lethal; D006321:Heart / Q000196:embryology*; D006410:Hematopoiesis / Q000235:genetics; D006579:Heterozygote; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011956:Receptors, Cell Surface; D013683:Telangiectasia, Hereditary Hemorrhagic / Q000235:genetics / Q000503:physiopathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D018388:Codon, Terminator; D017931:DNA Primers; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1