title: Detection of genomic variability in different populations of the cattle tick Boophilus microplus in southern Brazil.
abstract: DNA from seven isolates of the cattle tick Boophilus microplus was analyzed by restriction fragment length polymorphism (RFLP). Three different cDNA clones, named P-9, P-25 and CP-12, isolated from a B. microplus cDNA library, were used as DNA probes. DNA sequences of P-9 have high similarity to ribosomal genes, whereas P-25 does not show significant homology with known sequences within databases. CP-12 is a cDNA clone encoding a cysteine endopeptidase gene. A limited degree of polymorphism was detected with P-9 and P-25, while CP-12 showed a different pattern of bands for each tick isolate. These findings suggest the existence of a complex genotypic diversity of the tick B. microplus population in endemic regions.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: D T
authors.initials: DT
authors.lastname: Passos
authors.forename: C A
authors.initials: CA
authors.lastname: Ferreira
authors.forename: S S
authors.initials: SS
authors.lastname: da Silva
authors.forename: M F
authors.initials: MF
authors.lastname: Richter
authors.forename: L S
authors.initials: LS
authors.lastname: Ozaki
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D004247:DNA / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D015246:Deoxyribonuclease EcoRI / Q000737:chemistry; D005260:Female; D015723:Gene Library; D014644:Genetic Variation / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification; D013987:Ticks / Q000737:chemistry / Q000235:genetics*
chemical_list: D015342:DNA Probes; D012313:RNA; D004247:DNA; D015246:Deoxyribonuclease EcoRI