title: Influence of arginine dietary supplementation on healing colonic anastomosis in the rat.
abstract: BACKGROUND
  This study sought to determine whether dietary arginine influences colonic anastomotic healing in the rat model.
  
  
  METHODS
  Three groups of 42 Sprague-Dawley rats were fed 0, 1, and 3 percent arginine diets for three preoperative and three postoperative days. Animals underwent transection of the transverse colon with hand-sewn anastomosis. Subgroups of 14 animals in each dietary group were killed on postoperative Days 6, 10, or 14, and bursting pressures, histologic inflammation, and collagen content were compared.
  
  
  RESULTS
  Mean anastomotic bursting pressures on postoperative Day 6 were lower for the 0 percent arginine group than the 1 and 3 percent arginine groups (mean +/- standard error of the mean = 134+/-6 mm Hg, 164+/-7 mm Hg, and 166+/-7 mm Hg, respectively; P<0.0005). On Days 10 and 14, no significant differences in bursting pressures were noted between arginine diets. Mean bursting pressures on postoperative Day 6 (155+/-4 mm Hg) were significantly lower than on Days 10 (204+/-5 mm Hg) and 14 (217+/-6 mm Hg; P<0.001) for all arginine diets. Microscopic evaluation of the anastomoses did not show significant differences in inflammation or collagen content between arginine diets. Collagen content in all dietary groups peaked at Day 10.
  
  
  CONCLUSIONS
  Perioperative arginine deficiency in the rat model is associated with impaired anastomotic healing during the first week, as reflected by lower bursting pressures. Arginine supplementation to 3 percent does not improve bursting pressures above those found in the usual 1 percent arginine diet at 6, 10, or 14 days. Bursting pressures plateau by Day 10 regardless of perioperative dietary arginine, whereas collagen content peaks at Day 10 after six-day perioperative arginine diet manipulation.
authors.affiliation: Section of Colon and Rectal Surgery, Creighton University School of Medicine, Omaha, Nebraska, USA.
authors.forename: M
authors.initials: M
authors.lastname: Shashidharan
authors.forename: K M
authors.initials: KM
authors.lastname: Lin
authors.forename: C A
authors.initials: CA
authors.lastname: Ternent
authors.forename: T C
authors.initials: TC
authors.lastname: Smyrk
authors.forename: A G
authors.initials: AG
authors.lastname: Thorson
authors.forename: G J
authors.initials: GJ
authors.lastname: Blatchford
authors.forename: M A
authors.initials: MA
authors.lastname: Christensen
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000627:therapeutic use*; D003092:Colitis / Q000473:pathology; D003094:Collagen / Q000032:analysis; D003106:Colon / Q000737:chemistry / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D008297:Male; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012421:Rupture; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D003094:Collagen; D001120:Arginine