Toremifene increases the expression of intercellular adhesion molecule-1 (ICAM-1) on MCF-7 breast cancer cells and Jurkat cells.
The present study was conducted to reveal the effect of the nonsteroidal anti-oestrogen toremifene on the expression of cell-surface molecules involved in the immunogenicity of tumours or the sensitivity of tumour cells to apoptotic cell death. We studied the effect of toremifene on the expression of HLA-DR, ICAM-1, costimulatory molecules CD80 and CD86, and the tumour necrosis factor receptor (TNF-R) family molecules CD27, CD30, CD40, TNF-R1, TNF-R2 and Fas (CD95) on MCF-7 breast cancer and Jurkat T cells. In addition, the effect of toremifene on Fas-mediated apoptosis was studied. Toremifene did not affect Fas expression or Fas-mediated apoptosis in Fas-resistant MCF-7 or Fas-sensitive Jurkat cells, but was found to increase the expression of ICAM-1 in both cell lines. In addition, toremifene increased the expression of CD40 and CD80 on MCF-7 cells. The expression of ICAM-1 in tumours plays an important role in the interaction of tumour cells and effector cells of the immune system. Therefore, we suggest that toremifene may modulate the immunogenicity of tumour cells by increasing the expression of ICAM-1.
Komi
J
J
Turku Immunology Centre, Department of Medical Microbiology, Turku University, Turku, Finland.
Lassila
O
O
D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D018122:B7-1 Antigen / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D019013:CD40 Antigens / Q000378:metabolism; D003630:Daunorubicin / Q000494:pharmacology; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D019169:Jurkat Cells; D011994:Recombinant Proteins; D020845:Selective Estrogen Receptor Modulators / Q000494:pharmacology*; D017312:Toremifene / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000378:metabolism
D018931:Antineoplastic Agents, Hormonal; D018122:B7-1 Antigen; D019013:CD40 Antigens; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D020845:Selective Estrogen Receptor Modulators; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1; D017312:Toremifene; D007371:Interferon-gamma; D003630:Daunorubicin