title: Modulation of bovine serum amine oxidase activity by hydrogen peroxide.
abstract: Bovine serum amine oxidase (BSAO), reduced by excess amine under limited turnover conditions, was over 80% inactivated by H(2)O(2) upon oxygen exhaustion. The UV-Vis spectrum and the reduced reactivity with carbonyl reagents showed that the cofactor topaquinone (TPQ) was stabilized in reduced form. The protein large M(r) (170 kDa) prevented the identification of modified residues by amino acid analyses. Minor changes of the Cu(2+) EPR signal and the formation of a radical at g = 2.001, with intensity a few percent of that of the Cu(2+) signal, unaffected by a temperature increase, suggest that Cu(2+)-bound histidines were not oxidized and the radical was not the Cu(+)-semiquinolamine in equilibrium with Cu(2+)-aminoquinol. It may derive from the modification of a conserved residue in proximity of the active site, possibly the tyrosine at hydrogen-bonding distance of TPQ C-4 ionized hydroxyl. The inactivation reaction appears to be a general feature of copper-containing amine oxidases. It may be part of an autoregulatory process in vivo, possibly relevant to cell adhesion and redox signaling.
authors.affiliation: Centro di Biologia Molecolare del CNR, Università "La Sapienza,", P. le A. Moro 5, Rome, 00185, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Pietrangeli
authors.forename: S
authors.initials: S
authors.lastname: Nocera
authors.forename: P
authors.initials: P
authors.lastname: Fattibene
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Mondovì
authors.forename: L
authors.initials: L
authors.lastname: Morpurgo
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D008970:Molecular Weight; D013096:Spermine / Q000494:pharmacology
chemical_list: D001798:Blood Proteins; D013096:Spermine; D006861:Hydrogen Peroxide; D006631:Amine Oxidase (Copper-Containing)