Cloning and characterization of a gene (arpA) from Aspergillus oryzae encoding an actin-related protein required for normal nuclear distribution and morphology of conidiophores.
We have isolated the arpA gene from Aspergillus oryzae as a homologue of the Neurospora crassa ro-4 gene. In N. crassa, mutations in the ro-4 gene, which encodes a major component of the dynactin complex Arp1, causes curling of hyphae and abnormalities in nuclear distribution. The arpA gene contains two introns and encodes a polypeptide of 381 amino acids, with a 78% sequence identity to the N. crassa Arp1. Overexpression of the arpA gene causes a defect in nuclear migration into elongating hyphae of germlings in A. oryzae. We constructed arpA disruptant strains of A. oryzae. The arpA null mutants showed poor growth and hyper-branched mycelia, as well as a nuclear distribution defect. Scanning electron microscopy revealed that the arpA null mutant has an aberrant conidiophore morphology with irregular phialides.
Hirozumi
K
K
Department of Biotechnology, The University of Tokyo, Japan.
Nakajima
H
H
Machida
M
M
Yamaguchi
M
M
Takeo
K
K
Kitamoto
K
K
D000199:Actins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005656:Fungal Proteins; D005800:Genes, Fungal; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010957:Plasmids
D000199:Actins; D004271:DNA, Fungal; D005656:Fungal Proteins