ADAM-TS8, a novel metalloprotease of the ADAM-TS family located on mouse chromosome 9 and human chromosome 11.
A disintegrin-like and metalloprotease domain with thrombospondin type I modules (ADAM-TS) describes a novel family of zinc metalloendopeptidases. Its members have a common domain organization, which includes, typically, a pre-pro-metalloprotease domain, a disintegrin-like domain, and one or more thrombospondin-like (TS) modules. We describe here the complete primary structure of mouse ADAM-TS8, through cloning of Adamts8 cDNA. This novel member of the family contains two TS modules and is highly similar in sequence and domain organization to three other recently described gene products, ADAM-TS5, ADAM-TS6, and ADAM-TS7. Adamts8 is expressed at low levels throughout development and in adult mouse lung and heart. Through analysis of an interspecific backcross panel, we place the Adamts8 locus on mouse chromosome 9 at a consensus position of 11 cM and its human ortholog, recently reported as the METH2 gene, on human chromosome 11q25.
Georgiadis
K E
KE
Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Hirohata
S
S
Seldin
M F
MF
Apte
S S
SS
D051722:ADAM Proteins; D000071096:ADAMTS Proteins; D000071116:ADAMTS5 Protein; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D003545:Cysteine / Q000737:chemistry; D006801:Humans; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D019699:Thrombospondins / Q000737:chemistry; D015032:Zinc / Q000378:metabolism
D019699:Thrombospondins; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human; C120807:ADAMTS8 protein, human; C498489:Adamts5 protein, mouse; C498472:Adamts8 protein, mouse; D008666:Metalloendopeptidases; D015032:Zinc; D003545:Cysteine