A screening ELISA for brucellosis in reindeer.
An enzyme-linked immunosorbent assay (ELISA) for the screening of brucellosis in reindeer was developed. The assay, which utilizes s-LPS from Brucella abortus as antigen and biotin-labelled rabbit antibody to reindeer immunoglobulin as detecting antibody, has a high specificity and sensitivity, as indicated in a validation with sera from reindeer cultured positive for Brucella suis biovar 4 and sera from reindeer free of brucellosis.
Asbakk
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K
Norwegian School of Veterinary Science, Department of Arctic Veterinary Medicine, Tromsø, Norway.
Gall
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Stuen
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D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood / Q000276:immunology*; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D002852:Chromatography, Ion Exchange / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D011817:Rabbits; D012053:Reindeer; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial