title: NMR assignments, secondary structure, and global fold of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea.
abstract: Calerythrin is a 20 kDa calcium-binding protein isolated from gram-positive bacterium Saccharopolyspora erythraea. Based on amino acid sequence homology, it has been suggested that calerythrin belongs to the family of invertebrate sarcoplasmic EF-hand calcium-binding proteins (SCPs), and therefore it is expected to function as a calcium buffer. NMR spectroscopy was used to obtain structural information on the protein in solution. Backbone and side chain 1H, 13C, and 15N assignments were obtained from triple resonance experiments HNCACB, HN(CO)CACB, HNCO, CC(CO)NH, and [15N]-edited TOCSY, and HCCH-TOCSY. Secondary structure was determined by using secondary chemical shifts and characteristic NOEs. In addition, backbone N-H residual dipolar couplings were measured from a spin-state selective [1H, 15N] correlation spectrum acquired from a sample dissolved in a dilute liquid crystal. Four EF-hand motifs with characteristic helix-loop-helix patterns were observed. Three of these are typical calcium-binding EF-hands, whereas site 2 is an atypical nonbinding site. The global fold of calerythrin was assessed by dipolar couplings. Measured dipolar couplings were compared with values calculated from four crystal structures of proteins with sequence homology to calerythrin. These data allowed us to recognize an overall similarity between the folds of calerythrin and sarcoplasmic calcium-binding proteins from the sandworm Nereis diversicolor and the amphioxus Branchiostoma lanceolatum.
authors.affiliation: Institute of Biotechnology/NMR Laboratory, University of Helsinki, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Aitio
authors.forename: A
authors.initials: A
authors.lastname: Annila
authors.forename: S
authors.initials: S
authors.lastname: Heikkinen
authors.forename: E
authors.initials: E
authors.lastname: Thulin
authors.forename: T
authors.initials: T
authors.lastname: Drakenberg
authors.forename: I
authors.initials: I
authors.lastname: Kilpeläinen
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D020832:EF Hand Motifs; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016930:Saccharopolyspora / Q000737:chemistry*; D012996:Solutions
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D012996:Solutions; C090958:calerythrin protein, Saccharopolyspora erythraea