Simultaneous measurement of cefuroxime in rat blood and brain by microdialysis and microbore liquid chromatography. Application to pharmacokinetics.
To characterize the pharmacokinetics of cefuroxime in rat blood and brain, microdialysis probes were inserted into the jugular vein and brain striatum, respectively. Cefuroxime (20 mg/kg, i.v.) was administered via the femoral vein. Blood microdialysates were automatic injected onto microbore liquid chromatography via an on-line injectors. The mobile phase consisted of methanol-100 mM monosodium phosphoric acid (25:75, v/v, pH 5.0) with a flow-rate of 0.05 ml/min. Ultraviolet detector was set at a wavelength of 280 nm for cefuroxime. The present assay enhanced the detection sensitivity and enabled the determination of cefuroxime down to 5 ng/ml. The pharmacokinetic data demonstrated that the area under the concentration curve (AUC) ratio of unbound cefuroxime in rat brain and blood was about 4.2% after cefuroxime (20 mg/kg, i.v.) administration. These results provided further evidence that cefuroxime could penetrate the blood-brain barrier.
Tsai
T H
TH
Department of Pharmacology, National Research Institute of Chinese Medicine, Taipei, Taiwan. thtsai@cma23.nricm.edu.tw
Cheng
F C
FC
Chen
K C
KC
Chen
Y F
YF
Chen
C F
CF
D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002444:Cefuroxime / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D002511:Cephalosporins / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008297:Male; D000432:Methanol; D017551:Microdialysis; D010756:Phosphoric Acids; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D002511:Cephalosporins; D010756:Phosphoric Acids; C030242:phosphoric acid; D002444:Cefuroxime; D000432:Methanol