title: The myristoylated protein rapsyn is cotargeted with the nicotinic acetylcholine receptor to the postsynaptic membrane via the exocytic pathway.
abstract: Rapsyn, a 43 kDa protein required to cluster nicotinic acetylcholine receptors (AChRs) at the neuromuscular junction, is tightly associated with the postsynaptic membrane via an N-terminal myristoylated site. Recent studies have shown that some acylated proteins associate with the exocytic pathway to become targeted to their correct destination. In this work, we used Torpedo electrocyte to investigate the intracellular routing of rapsyn compared to those of AChR and Na,K-ATPase, the respective components of the innervated and noninnervated membranes. We previously demonstrated that these latter two proteins are sorted and targeted to plasma membrane via distinct populations of post-Golgi vesicles (). Biochemical and immunoelectron microscopy analyses of various populations of post-Golgi vesicles immunopurified with magnetic beads led us to identify post-Golgi transport vesicles containing both rapsyn and AChR. These data suggest that rapsyn, as for AChR, specifically follows the exocytic pathway. Furthermore, immunogold-labeling experiments provided in situ evidence that AChR and rapsyn are cotransported in the same post-Golgi vesicles. Taken together, our observations suggest that rapsyn and AChR are cotargeted to the postsynaptic membrane.
authors.affiliation: Biologie Cellulaire des Membranes, Département de Biologie Supramoléculaire et Cellulaire, Institut Jacques Monod, Unité Mixte Recherche 7592, Centre National de la Recherche Scientifique, Universités Paris 6 et Paris 7, 75251, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Marchand
authors.forename: F
authors.initials: F
authors.lastname: Bignami
authors.forename: F
authors.initials: F
authors.lastname: Stetzkowski-Marden
authors.forename: J
authors.initials: J
authors.lastname: Cartaud
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D004557:Electric Organ / Q000502:physiology*; D005089:Exocytosis; D016253:Microscopy, Immunoelectron; D009124:Muscle Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D019814:Myristic Acid / Q000378:metabolism; D015388:Organelles / Q000502:physiology / Q000648:ultrastructure; D011978:Receptors, Nicotinic / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure; D013570:Synaptic Membranes / Q000502:physiology; D014101:Torpedo
chemical_list: D009124:Muscle Proteins; D011978:Receptors, Nicotinic; C051609:peripheral membrane protein 43K; D019814:Myristic Acid