Changes in nicotinic acetylcholine receptor subunit mRNAs and nicotinic binding in spontaneously hypertensive stroke prone rats.
We have studied nicotinic acetylcholine receptors in spontaneously hypertensive 'stroke prone' (SHsp) rats. We found a significant decrease in 125I-alpha-bungarotoxin binding and alpha7 subunit mRNA levels in cortical areas of the SHsp rats with respect to Wistar Kyoto (WKy) normotensive rats. Antihypertensive drug treatment counteracted these changes in cerebral cortex but not in hippocampus. No significant change was instead found in [3H]-epibatidine binding and alpha4 and beta2 subunit mRNA levels. SHsp rats showed decreased latency at the active avoidance test and transiently increased threshold at both hot-plate and tail-flick tests in comparison with WKy rats. None of these behavioral parameters was correlated with 125I-alpha-bungarotoxin binding in cortical areas. In conclusion, present data show a preferential impairment of alpha-bungarotoxin sensitive nAChRs in SHsp rats.
Ferrari
R
R
Department of Biomedical Sciences, University of Modena and Reggio Emilia, Italy.
Frasoldati
A
A
Leo
G
G
Torri
C
C
Zini
I
I
Agnati
L F
LF
Zoli
M
M
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001362:Avoidance Learning / Q000502:physiology; D002038:Bungarotoxins / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D009538:Nicotine / Q000378:metabolism*; D017288:Pain Threshold; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics / Q000378:metabolism*; D011921:Rats, Inbred WKY; D011930:Reaction Time; D011978:Receptors, Nicotinic / Q000235:genetics* / Q000378:metabolism; D012016:Reference Values; D020521:Stroke / Q000235:genetics
D000959:Antihypertensive Agents; D002038:Bungarotoxins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D009538:Nicotine