title: The induction of skeletal muscle hypertrophy by a ski transgene is promoter-dependent.
abstract: The chicken c-ski gene expresses at least three alternatively spliced messages. Transgenic mice expressing proteins from cDNA corresponding to two of these messages (FB27 and FB29) under the control of a murine sarcoma virus (MSV) long terminal repeat (LTR) express the transgene in skeletal muscle and develop a muscular phenotype. Both a biologically active form of c-ski and the MSV LTR are required for the development of the muscular phenotype. The normal c-ski gene linked to two other tissue-specific promoters failed to induce muscle growth in transgenic mice, as did an inactive mutant of c-ski expressed under the control of the MSV LTR.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: P
authors.initials: P
authors.lastname: Sutrave
authors.forename: J M
authors.initials: JM
authors.lastname: Leferovich
authors.forename: A M
authors.initials: AM
authors.lastname: Kelly
authors.forename: S H
authors.initials: SH
authors.lastname: Hughes
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D006984:Hypertrophy / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D018995:Myosin Heavy Chains / Q000235:genetics; D010641:Phenotype; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000502:physiology*; D012260:Ribonucleases / Q000378:metabolism; D009053:Sarcoma Viruses, Murine / Q000235:genetics; D020079:Terminal Repeat Sequences / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; C512653:Ski protein, mouse; D012260:Ribonucleases; D018995:Myosin Heavy Chains