Effects of intraocular or systemic administration of neutralizing antibody against vascular endothelial growth factor on the murine experimental model of retinopathy.
Vascular endothelial growth factor (VEGF), the strongest known angiogenic cytokine and also a potent enhancer of vascular permeability, is closely associated with diabetic ocular complications and other intraocular neovascular diseases. The therapeutic effect of VEGF-neutralizing antibody on oxygen-induced retinopathy in an experimental murine model of proliferative retinopathy was investigated. Intraocular and systemic injection of the antibody resulted in 46% and 18% reductions in the number of nuclei of newly formed vessels of this model, respectively. The results demonstrated that a neutralizing antibody against VEGF was highly effective in the treatment of intraocular neovascularization and suggested possible modes of therapy in human intraocular neovascular diseases, including diabetic proliferative retinopathy.
Sone
H
H
Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Japan. hsone@md.tsukuba.ac.jp
Kawakami
Y
Y
Segawa
T
T
Okuda
Y
Y
Sekine
Y
Y
Honmura
S
S
Segawa
T
T
Suzuki
H
H
Yamashita
K
K
Yamada
N
N
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D003930:Diabetic Retinopathy / Q000628:therapy; D004195:Disease Models, Animal; D016228:Endothelial Growth Factors / Q000037:antagonists & inhibitors* / Q000276:immunology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007279:Injections, Subcutaneous; D008222:Lymphokines / Q000037:antagonists & inhibitors* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000633:toxicity; D011247:Pregnancy; D011817:Rabbits; D015861:Retinal Neovascularization / Q000628:therapy; D012178:Retinopathy of Prematurity / Q000628:therapy*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D000906:Antibodies; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D010100:Oxygen