title: Reactivation of human herpesvirus 6 by infection of human herpesvirus 7.
abstract: We have attempted to reactivate human herpesvirus 6 (HHV-6) by infection with HHV-7 using childhood exanthem subitum patients in vitro. Peripheral blood mononuclear cells (PBMCs) were collected from children who had a history of exanthem subitum(ES) by HHV-6 and were infected by human herpesvirus 7 (HHV-7) in vitro. The antigen positive rate to HHV-6 started to increase 7 days after the infection and reached a maximum by Day 15 using an immunofluorescence antibody test. The copy number of HHV-6 DNA also increased in the samples in 10 days after infection in vitro. No antigen or increase in DNA was detected in PBMCs, that were mock-infected or infected with supernatant of stock virus after ultracentrifugation, suggesting that an infection by HHV-7 is necessary to reactivate HHV-6. In the paired sera samples during the acute and the convalescent phases of ES, seven to ten bands, that were specific for HHV-6, were recognized in samples from the acute phase, and at least 5 dominant polypeptides were found more intensively after HHV-7 infection.
authors.affiliation: Department of Developmental Medicine (Pediatrics) D-5, Osaka University Graduate School of Medicine, Suita City, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tanaka-Taya
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: N
authors.initials: N
authors.lastname: Nakagawa
authors.forename: R
authors.initials: R
authors.lastname: Inagi
authors.forename: H
authors.initials: H
authors.lastname: Miyoshi
authors.forename: T
authors.initials: T
authors.lastname: Sunagawa
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.forename: K
authors.initials: K
authors.lastname: Yamanishi
mesh_terms: D015139:Blotting, Southern; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000097:blood / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000254:growth & development / Q000502:physiology*; D016199:Herpesvirus 7, Human / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000821:virology; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D014775:Virus Activation
chemical_list: D004279:DNA, Viral