Characterization and purification of porcine small intestinal mucus receptor for Escherichia coli K88ac fimbrial adhesin.
The objectives of this study were to investigate the nature of, and to purify K88ac fimbrial adhesin-specific receptors in the mucus from the small intestine of piglet. Adhesion was studied by incubating (3)H-labeled Escherichia coli with mucus that were treated with or without pronase, proteinase, trypsin or sodium metaperiodate. The results indicated that treatment with either proteolytic enzymes or sodium metaperiodate (to oxidize sugars) significantly reduced E. coli K88ac or K88+MB adhesion to the mucus, suggesting that the K88ac and K88+MB specific receptors in this preparation were, at least in part, glycoprotein in nature. The K88+MB fimbriae specific receptor was purified using affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified K88+MB specific receptor together with the above data suggested that the receptor from the mucus of the small intestine of the pig was a 80-kDa glycoprotein.
Jin
L Z
LZ
Department of Animal Science, University of Manitoba, Winnipeg, Canada.
Marquardt
R R
RR
Baidoo
S K
SK
Frohlich
A A
AA
D018830:Adhesins, Escherichia coli / Q000378:metabolism*; D000818:Animals; D000942:Antigens, Bacterial; D000954:Antigens, Surface / Q000378:metabolism*; D001422:Bacterial Adhesion; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D007421:Intestine, Small / Q000382:microbiology; D009093:Mucus / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D011971:Receptors, Immunologic / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D011971:Receptors, Immunologic; C047105:bacterial adhesin receptor; D036781:Fimbriae Proteins