title: Morphological and physiological effects of long duration infusion of strychnine into the organ of Corti.
abstract: Acute strychnine administration has long been used as a method to eliminate the effects of efferent activity. It has been shown that long after termination of chronic strychnine infusion into the cochlea, the ear becomes more susceptible to acoustic trauma suggesting that chronic strychnine infusion results in long lasting or permanent disruption of efferent function. Much research has been directed towards the functional significance of the olivocochlear system. However, there is little information concerning the effect of long duration inactivation of the medial olivocochlear system in an awake behaving animal. This study was designed to determine the structural and functional consequences of inactivation of the efferents by chronic infusion of strychnine into the cochlear perilymph of guinea pigs for two weeks via an osmotic pump. Physiological evaluations showed that the strychnine infusion eliminated the efferent induced reduction of the cochlear whole-nerve action potential three weeks after cessation of strychnine infusion. Contralateral efferent function remained unaltered. Histological evaluation at the light and electron microscopic levels revealed disoriented efferent synapses under the outer hair cells.
authors.affiliation: Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: D F
authors.initials: DF
authors.lastname: Dolan
authors.forename: T
authors.initials: T
authors.lastname: Yamasoba
authors.forename: E
authors.initials: E
authors.lastname: Leonova
authors.forename: L A
authors.initials: LA
authors.lastname: Beyer
authors.forename: Y
authors.initials: Y
authors.lastname: Raphael
mesh_terms: D000161:Acoustic Stimulation; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001306:Auditory Pathways / Q000187:drug effects; D001309:Auditory Threshold / Q000187:drug effects; D004558:Electric Stimulation; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000648:ultrastructure; D015918:Infusion Pumps, Implantable; D007263:Infusions, Parenteral; D008854:Microscopy, Electron; D009925:Organ of Corti / Q000187:drug effects*; D010498:Perilymph; D013331:Strychnine / Q000008:administration & dosage / Q000633:toxicity*; D013569:Synapses / Q000187:drug effects; D014851:Wakefulness
chemical_list: D013331:Strychnine