title: Mutatect: a mouse tumour model for detecting radiation-induced mutations in vivo.
abstract: A new mouse model (Mutatect) that permits detection of mutations at the hprt (hypoxanthine phosphoribosyltransferase) locus is described. It is highly sensitive to detection of mutants induced by clastogenic agents such as ionizing radiation. MN-11 cells are grown as a subcutaneous tumour in C57BL/6 mice for a period of 2 weeks, during which time they can be exposed to mutagenic treatments. Cells taken from the animal are cultured ex vivo and 6-thioguanine (6-TG)-resistant mutant clones can be readily identified and scored. This model system may have special utility for detecting multi-locus deletion events (chromosomal mutations) induced by high LET forms of radiation that might be encountered in space.
authors.affiliation: Ottawa Regional Cancer Centre, Ottawa, Canada. birnboim@uottawa.ca
authors.forename: H C
authors.initials: HC
authors.lastname: Birnboim
authors.forename: D
authors.initials: D
authors.lastname: Wilkinson
authors.forename: J K
authors.initials: JK
authors.lastname: Sandhu
authors.forename: J R
authors.initials: JR
authors.lastname: McLean
authors.forename: W
authors.initials: W
authors.lastname: Ross
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004303:Dosage Compensation, Genetic; D004351:Drug Resistance / Q000528:radiation effects; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics*; D005796:Genes / Q000528:radiation effects; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D018499:Linear Energy Transfer; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008810:Mice, Inbred C57BL; D009152:Mutagenicity Tests; D009363:Neoplasm Proteins / Q000235:genetics*; D009368:Neoplasm Transplantation; D009630:Nondisjunction, Genetic; D013026:Space Flight; D013866:Thioguanine / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014960:X Chromosome / Q000528:radiation effects*
chemical_list: D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D008748:Methylcholanthrene; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine