Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence In situ hybridization probes.
A blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81. 5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with either probe and were identified as Mycobacterium xenopi, Mycobacterium fortuitum, or Mycobacterium flavescens.
Drobniewski
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PHLS Mycobacterium Reference Unit, Department of Microbiology, Dulwich Public Health Laboratory, Guy's King's and St Thomas' Medical School, Dulwich Hospital, London SE22 8QF, United Kingdom. francis.drobniewski@kcl.ac.uk
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Harris
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D001431:Bacteriological Techniques; D003470:Culture Media; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009161:Mycobacterium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000175:diagnosis*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D020135:Peptide Nucleic Acids; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D003470:Culture Media; D020135:Peptide Nucleic Acids; D011933:Reagent Kits, Diagnostic