Neurotrophic role of interleukin-6 and soluble interleukin-6 receptors in N1E-115 neuroblastoma cells.
Interleukin 6 (IL-6) plays a role in physiological and pathophysiological processes in neuronal cells. We studied whether IL-6 plays a role in neuroblastoma cells in culture. These studies demonstrate that N1E-115 cells constitutively express IL-6 but not IL-6R. Exogenous IL-6 stimulated neuronal proliferation in a dose-dependent manner. Under serum-free conditions soluble IL-6 receptors (sIL-6R) alone or in combination with IL-6 exerted significant proliferative effects, while IL-6 alone failed to promote cell proliferation. Neutralizing anti-IL-6 antibody caused a 30-40% reduction in IL-6 mediated proliferation. Our results suggest the importance of IL-6/sIL-6R for proliferation and survival of N1E-115 adrenergic neuroblastoma cells.
Knezevic-Cuca
J
J
The Strelitz Diabetes Institutes, Department of Internal Medicine, Eastern Virginia Medical School, Norfolk, USA.
Stansberry
K B
KB
Johnston
G
G
Zhang
J
J
Keller
E T
ET
Vinik
A I
AI
Pittenger
G L
GL
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001769:Blood / Q000378:metabolism; D001790:Blood Physiological Phenomena; D002417:Cattle / Q000097:blood; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D016895:Culture Media, Serum-Free; D004305:Dose-Response Relationship, Drug; D005312:Fetal Blood / Q000502:physiology; D015850:Interleukin-6 / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D009414:Nerve Growth Factors / Q000502:physiology*; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D009994:Osmolar Concentration; D019947:Receptors, Interleukin-6 / Q000502:physiology*; D012995:Solubility; D014407:Tumor Cells, Cultured
D000906:Antibodies; D016895:Culture Media, Serum-Free; D015850:Interleukin-6; D009414:Nerve Growth Factors; D019947:Receptors, Interleukin-6