The spatial localization of T-DNA insertions in petunia interphase nuclei: consequences for chromosome organization and transgene insertion sites.
In an earlier fluorescent in-situ hybridization (FISH) study on petunia (ten Hoopen et al. 1996), we found a considerable discrepancy between the genetic map and the physical map with respect to T-DNA insertions on metaphase chromosomes. For some transgenes we found a preference to integrate near the telomeres. Here, we studied the spatial position of transgenes in interphase nuclei by FISH and 3D-confocal microscopy to elucidate a possible structural preference for the nuclear localization of transgenes. Three transgenes located near telomeres on three different metaphase chromosomes showed a much more internal distribution in interphase root meristem than the telomeres, whereas a proximal transgene appeared to be distributed in a random fashion. The results point to local differences in chromatin compacting along a chromosome. These differences might explain a preference for T-DNA insertion in distal regions of the chromosome.
ten Hoopen
R
R
Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, The Netherlands.
Montijn
B M
BM
Veuskens
J T
JT
Oud
O J
OJ
Nanninga
N
N
D002467:Cell Nucleus / Q000378:metabolism*; D002875:Chromosomes; D004269:DNA, Bacterial / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D019657:Solanaceae / Q000235:genetics* / Q000648:ultrastructure; D019076:Transgenes
D004269:DNA, Bacterial; C036483:T-DNA