title: Lifetime persistence and clonality of chromosome aberrations in the peripheral blood of mice acutely exposed to ionizing radiation.
abstract: As the measurement of chromosomal translocations increases in popularity for quantifying prior radiation exposure, information on the possible decline of these "stable" aberrations over time is urgently needed. We report here information about the persistence of radiation-induced chromosome aberrations in vivo over the life span of a rodent. Female C57BL/6 mice were given a single whole-body acute exposure of 0, 1, 2, 3 or 4 Gy (137)Cs gamma rays at 8 weeks of age. Chromosome aberrations were analyzed from peripheral blood samples at various intervals between 1 day and 21 months after exposure. Aberrations were detected by painting chromosomes 2 and 8. Translocations decreased dramatically during the first 3 months after irradiation, beyond which time the frequencies remained relatively constant out to 1 year, when the effects of aging and clonal expansion became significant. Both reciprocal and nonreciprocal translocations increased with age in the unexposed control animals and were involved in clones. As expected of unstable aberrations, dicentrics decreased rapidly after exposure and reached baseline levels within 3 months. These results indicate that the persistence of translocations induced by ionizing radiation is complicated by aging and clonal expansion and that these factors must be considered when quantifying translocations at long times after exposure. These results have implications for biological dosimetry in human populations.
authors.affiliation: Toxicology and Molecular Biology Branch, MS 3014, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, West Virginia 26505, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Spruill
authors.forename: D O
authors.initials: DO
authors.lastname: Nelson
authors.forename: M J
authors.initials: MJ
authors.lastname: Ramsey
authors.forename: J
authors.initials: J
authors.lastname: Nath
authors.forename: J D
authors.initials: JD
authors.lastname: Tucker
mesh_terms: D000375:Aging / Q000235:genetics; D000818:Animals; D002588:Cesium Radioisotopes; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D002875:Chromosomes / Q000528:radiation effects*; D002999:Clone Cells / Q000528:radiation effects; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D005720:Gamma Rays; D008214:Lymphocytes / Q000473:pathology / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D011829:Radiation Dosage; D014178:Translocation, Genetic; D014916:Whole-Body Irradiation
chemical_list: D002588:Cesium Radioisotopes