title: [Inhibition of mitogen-activated protein kinase cascade by baicalein, a flavonoid of natural origin].
abstract: Mitogen-activated protein kinase (MAPK) is a family of serine/threonine kinase that appears to be a component common to signalling pathway initiated by a wide range of factors including hormones, differentiation factors and mitogens. Baicalein is a flavonoid derived from the root of Scutellaria baicaleins. From searching the inhibitors of prostaglandin synthesis in C6 rat glioma cells, we found that baicalein had a potent inhibitory activity of prostaglandin synthesis induced by either histamine or A23187, a Ca2+ ionophore. Baicalein inhibited histamine- and A23187-induced phosphorylation of MAPK in the cells, which was known to cause the phosphorylation of cytosolic phospholipase A2. Baicalein also inhibited the phosphorylation of MAPK kinase (MEK) induced by histamine or A23187 in the cells. In vitro kinase assay, baicalein inhibited the activities of raf but not MEK. These results imply that baicalein is a specific inhibitor of MAPK cascade, acting on phosphorylation of MEK by raf.
authors.affiliation: Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nakahata
authors.forename: R
authors.initials: R
authors.lastname: Kyo
authors.forename: M
authors.initials: M
authors.lastname: Kutsuwa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohizumi
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005910:Glioma / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D010766:Phosphorylation; D011448:Prostaglandin Antagonists / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D007476:Ionophores; D011448:Prostaglandin Antagonists; D000001:Calcimycin; C006680:baicalein; D006632:Histamine; D020928:Mitogen-Activated Protein Kinases