DNA sequencing in a monolithic microchannel device.
We present 50 cm long microchannels in a monolithic device for high resolution, long read-length DNA sequencing. These devices were fabricated and bonded in borofloat glass using unconventional photolithography techniques with 48-188 independent, straight microchannels. The microchannel DNA separation was tested with POP-6 polymer and a DNA sequencing ladder separated at room temperature and 200 V/cm. Single-base resolution greater than 600 bases was achieved and the sequence base called to 640 bases with 98% accuracy. Under the same experimental conditions, the performance of the microchip was identical to a fused-silica capillary with similar cross-sectional area.
Backhouse
C
C
PE Biosystems, Foster City, CA 94404, USA.
Caamano
M
M
Oaks
F
F
Nordman
E
E
Carrillo
A
A
Johnson
B
B
Bay
S
S
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods*; D004864:Equipment and Supplies; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA