title: Structural changes in the promoter region mediate transvection at the sn-glycerol-3-phosphate dehydrogenase gene of Drosophila melanogaster.
abstract: Trans effects at the sn-glycerol-3-phosphate dehydrogenase locus (Gpdh) of Drosophila melanogaster give rise to an increase in GPDH activity and mRNA from the wild-type allele in heterozygotes with some low-activity alleles. Either the low-activity alleles that induce the effect have a defective P-element inserted between the promoter and a downstream intronic enhancer element or the promoter region is deleted. The trans effect is pairing dependent, characteristic of transvection at some other loci. The defective P-elements that mediate transvection are located between the promoter and at least up to 6 bp downstream of the transcription start site. Transvection at Gpdh appears similar to the "enhancer action in trans" mode at the yellow locus.
authors.affiliation: Molecular Genetics and Evolution Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT, Australia.
authors.forename: J B
authors.initials: JB
authors.lastname: Gibson
authors.forename: D S
authors.initials: DS
authors.lastname: Reed
authors.forename: S
authors.initials: S
authors.lastname: Bartoszewski
authors.forename: A V
authors.initials: AV
authors.lastname: Wilks
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D006579:Heterozygote; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D014158:Transcription, Genetic
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; C060849:z protein, Drosophila; D005993:Glycerolphosphate Dehydrogenase