Evaluation of sandwich ELISA galactomannan test in samples of positive LA test and positive aspergillus antibody.
OBJECTIVE
The detection of circulating Aspergillus galactomannan antigen is a useful tool for serodiagnosis of aspergillosis. However, the latex agglutination test for the detection of galactomannan is not completely reliable due to it's low sensitivity. The sandwich ELISA was developed to achieve high sensitivity.


METHODS
The sandwich immunocapture ELISA was evaluated by testing 56 sero-positive and 56 sero-negative samples of circulating galactomannan detected by LA test retrospectively.


RESULTS
Sixty of the samples were positive for galactomannan as measured by sandwich ELISA. Fifteen samples out of 56 samples negative by LA test were positive by ELISA and 4 samples out of 56 samples positive by LA test were negative by ELISA. Among 47 serum samples positive for anti-Aspergillus antibody, 14 samples were positive by ELISA.


CONCLUSIONS
In conclusion, galactomannan may be detected in more samples of by the new sandwich ELISA than by LA test.
Maesaki
S
S
Second Department of Internal Medicine, Nagasaki University School of Medicine.
Kawamura
S
S
Hashiguchi
K
K
Hossain
M A
MA
Sasaki
E
E
Miyazaki
Y
Y
Tomono
K
K
Tashiro
T
T
Kohno
S
S
D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000097:blood*; D001228:Aspergillosis / Q000097:blood* / Q000175:diagnosis*; D001230:Aspergillus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D007841:Latex Fixation Tests; D008351:Mannans / Q000276:immunology*; D011237:Predictive Value of Tests
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D008351:Mannans; C012990:galactomannan; D005690:Galactose