High-speed chiral separations on microchip electrophoresis devices.
High-speed electrophoretic chiral separations have been successfully performed in a microfabricated device by employing cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC). Utilizing short separation channels and relatively high field strengths in combination with small volume-defined injection plugs, and operating in counter-electroosmotic flow conditions, fast and efficient separations of fluorescein insothiocyanate (FITC)-labeled amino acid enantiomers were obtained. Analysis time ranged from 75 s for the most basic amino acids to 160 s for the most acidic ones with associated efficiencies from 7000 up to 28 000 effective plates (100 000 to 395 000 plates/m). Buffer parameters were varied in order to study the effect on chiral resolution. A buffer system consisting of 100 mM borate (pH 9.4), 30 mM of SDS, and 10 mM gamma-CD as chiral selector provided adequate resolution of the majority of FITC-amino acid enantiomers tested.
Rodríguez
I
I
Department of Chemistry, National University of Singapore, Kent Ridge, Republic of Singapore.
Jin
L J
LJ
Li
S F
SF
D000818:Animals; D002845:Chromatography / Q000379:methods; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000032:analysis*
D011506:Proteins