Simultaneous genotyping of human platelet antigens (HPA) 1 through 6 using new sequence-specific primers for HPA-5.
BACKGROUND
Polymerase chain reaction using sequence-specific primers is widely used for genotyping human platelet antigens (HPA). However, the results of HPA-5 genotyping are still problematic.


METHODS
New sequence-specific primers were designed for HPA-5 that, together with already published primers, allow simultaneous genotyping of HPA-1, -2, -3, -4, -5, and -6. The reliability of the described protocol was determined by using reference DNA samples as well as samples from healthy blood donors.


RESULTS
All primers produced specific amplification products. The genotype and the previously ascertained phenotype of the tested specimens were in concordance in all cases.


CONCLUSIONS
The described polymerase chain reaction protocol allows rapid, reliable, simultaneous genotyping of HPA-1, -2, -3, -4, -5, and -6.
Meyer
O
O
Blood Bank, Charité, Campus Virchow-Klinikum, Medical Faculty of Humboldt University of Berlin, Germany.
Hildebrandt
M
M
Schulz
B
B
Blasczyk
R
R
Salama
A
A
D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001782:Blood Donors; D017931:DNA Primers / Q000097:blood*; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational
D016824:Antigens, Human Platelet; D017931:DNA Primers