title: Additional human papillomavirus types detected by the hybrid capture tube test among samples from women with cytological and colposcopical atypia.
abstract: The type specificity of the human papillomavirus (HPV) Hybrid Capture Tube (HCT) test was evaluated by using typing with PCR (MY09-MY11)-restriction fragment length polymorphism (RFLP) and sequencing. All samples HCT test positive for only low-risk HPV (n = 15) or only high-risk HPV (n = 102) were confirmed, whereas 9 of 12 HCT test double-positive samples contained only high-risk HPV types as determined by PCR-RFLP. Several high-risk HPV types (HPV-53, -58, -62, -66, -CP8304, and -MM4) not included in the HCT test were indeed detected, indicating a broader detection range with retained distinction between low-risk and high-risk HPV types.
authors.affiliation: Department of Microbiology, University Medical School of Debrecen, Debrecen, Hungary. konya@mibio.dote.hu
authors.forename: J
authors.initials: J
authors.lastname: Kónya
authors.forename: G
authors.initials: G
authors.lastname: Veress
authors.forename: A
authors.initials: A
authors.lastname: Juhász
authors.forename: K
authors.initials: K
authors.lastname: Szarka
authors.forename: T
authors.initials: T
authors.lastname: Sápy
authors.forename: Z
authors.initials: Z
authors.lastname: Hernádi
authors.forename: L
authors.initials: L
authors.lastname: Gergely
mesh_terms: D002584:Cervix Uteri / Q000473:pathology / Q000821:virology*; D003127:Colposcopy; D005260:Female; D006801:Humans; D008403:Mass Screening; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015347:RNA Probes; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis*
chemical_list: D015347:RNA Probes; D011933:Reagent Kits, Diagnostic