title: Three-dimensional protein NMR TROSY-type (15)N-resolved (1)H(N)-(1)H(N) NOESY spectra with diagonal peak suppression.
abstract: An earlier two-dimensional NOESY experiment with diagonal peak suppression in the (1)H(N)-(1)H(N) region is extended to three dimensions by including (15)N evolution while maintaining the TROSY approach throughout. The technique suppresses all anti-TROSY resonances by appropriate pulse sequence elements and for large molecules at high fields possible semi- and anti-TROSY artifacts are further suppressed by virtue of much shorter transverse relaxation times for these components. The new technique is demonstrated using an (15)N-labeled protein sample, RAP 17-97 (N-terminal domain of alpha2-macroglobulin Receptor Associated Protein), in H(2)O at 500 MHz.
authors.affiliation: Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, Valby, DK-2500, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Meissner
authors.forename: O W
authors.initials: OW
authors.lastname: SŁrensen
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009587:Nitrogen Isotopes; D011971:Receptors, Immunologic; D011973:Receptors, LDL
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D009587:Nitrogen Isotopes; D011971:Receptors, Immunologic; D011973:Receptors, LDL