title: Voluntary ethanol drinking during the first three postnatal weeks in lines of rats selectively bred for divergent ethanol preference.
abstract: BACKGROUND
  Using a procedure first developed by Hall (1979), we examined ethanol self-administration in preweanling pups from Wistar rats and in lines of rats selectively bred for divergent ethanol preference (alcohol-preferring P, alcohol-nonpreferring NP, high-alcohol-drinking HAD-1 and -2, and low-alcohol-drinking LAD-2) to determine if factors contributing to high and low alcohol intakes are present early in development.
  
  
  METHODS
  From postnatal days 5 to 20, nondeprived male and female rat pups received 30 min daily access to either water or a 15% (v/v) ethanol solution. In each daily session, pups were placed in a heated chamber containing Kimwipes soaked with a water or ethanol solution. Pups were weighed before and after each session, and intake levels were calculated as a percentage of body weight change.
  
  
  RESULTS
  Similar to previous reports, Wistar pups exhibited over a 2-fold higher level of ethanol ingestion than water on postnatal days 9 through 14, with ethanol intakes approaching 3 g/kg body weight. When the drinking patterns of the selected lines were examined, only the HAD replicate lines showed a comparable preference for ethanol versus water during the preweanling period. The ethanol consumption of P, NP, and LAD lines was not consistently distinguishable from water intake levels. To reveal whether early ethanol exposure would influence later ethanol drinking behavior, a subset of HAD and LAD rat pups received free-choice ethanol access upon weaning. Although the divergent ethanol preference between high- and low-alcohol-drinking lines was evident within the first 4 days of access (>4 g/kg/day for HAD; <2 g/kg/day for LAD), preweanling ethanol exposure did not alter the acquisition or maintenance of ethanol drinking in either line.
  
  
  CONCLUSIONS
  Overall, these results suggest that (a) the enhanced ethanol ingestion observed during the middle portion of the preweanling period is a robust phenomenon and generalizes across nonselected strains of rats, (b) selective breeding for divergent alcohol preference has arrested this age-specific effect in all but the HAD lines of rats, and (c) early ethanol exposure does not alter genetic dispositions for later high or low alcohol preference.
authors.affiliation: Department of Psychiatry, Indiana University School of Medicine, Indianapolis 46202-4887, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: McKinzie
authors.forename: R
authors.initials: R
authors.lastname: Cox
authors.forename: J M
authors.initials: JM
authors.lastname: Murphy
authors.forename: T K
authors.initials: TK
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Lumeng
authors.forename: W J
authors.initials: WJ
authors.lastname: McBride
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics* / Q000523:psychology; D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight / Q000187:drug effects / Q000235:genetics*; D002492:Central Nervous System Depressants / Q000494:pharmacology; D004326:Drinking; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012646:Self Administration / Q000523:psychology
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol