The structure of the nucleosome core particle of chromatin in chicken erythrocytes visualized by using atomic force microscopy.
The structure of the nucleosome core particle of chromatin in chicken erythrocytes has been examined by using AFM. The 146 bp of DNA wrapped twice around the core histone octamer are clearly visualized. Both the ends of entry/exit of linker DNA are also demonstrated. The dimension of the nucleosome core particles is approximately 1-4 nm in height and approximately 13-22 nm in width. In addition, superbeads (width of approximately 48-57 nm, height of approximately 2-3 nm) are occasionally revealed, two turns of DNA around the core particles are also detected.
Zhao
H
H
Shanghai Institute of Cell Biology, Chinese Academy of Sciences.
Zhang
Y
Y
Zhang
S B
SB
Jiang
C
C
He
Q Y
QY
Li
M Q
MQ
Qian
R L
RL
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000648:ultrastructure; D004912:Erythrocytes / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D009707:Nucleosomes / Q000235:genetics / Q000648:ultrastructure*
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA