Effective expression and purification of recombinant onconase, an antitumor protein.
Several members of the RNase A superfamily are endowed with antitumor activity, showing selective cytotoxicity toward several tumor cell lines. One of these is onconase, the smallest member of the RNase A superfamily, which is at present undergoing phase III clinical trials. We report here the expression of recombinant onconase in Escherichia coli inclusion bodies, the correct processing of the protein, followed by its purification in high yields. The recombinant protein has biological and catalytic properties identical to those of the natural enzyme.
Notomista
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E
Dipartimentó di Chimica Organica e Biologica, Università di Napoli Federico II, Via Mezzocannone 16, 80134, Naples, Italy.
Cafaro
V
V
Fusiello
R
R
Bracale
A
A
D'Alessio
G
G
Di Donato
A
A
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D009154:Mutation; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D012331:RNA, Fungal / Q000378:metabolism; D011894:Rana pipiens / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D012260:Ribonucleases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D012965:Sodium Chloride; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D004527:Egg Proteins; D011500:Protein Synthesis Inhibitors; D012331:RNA, Fungal; D011994:Recombinant Proteins; D012965:Sodium Chloride; D012260:Ribonucleases; C058703:ranpirnase