Reactivation of human herpesvirus 6 by infection of human herpesvirus 7.
We have attempted to reactivate human herpesvirus 6 (HHV-6) by infection with HHV-7 using childhood exanthem subitum patients in vitro. Peripheral blood mononuclear cells (PBMCs) were collected from children who had a history of exanthem subitum(ES) by HHV-6 and were infected by human herpesvirus 7 (HHV-7) in vitro. The antigen positive rate to HHV-6 started to increase 7 days after the infection and reached a maximum by Day 15 using an immunofluorescence antibody test. The copy number of HHV-6 DNA also increased in the samples in 10 days after infection in vitro. No antigen or increase in DNA was detected in PBMCs, that were mock-infected or infected with supernatant of stock virus after ultracentrifugation, suggesting that an infection by HHV-7 is necessary to reactivate HHV-6. In the paired sera samples during the acute and the convalescent phases of ES, seven to ten bands, that were specific for HHV-6, were recognized in samples from the acute phase, and at least 5 dominant polypeptides were found more intensively after HHV-7 infection.
Tanaka-Taya
K
K
Department of Developmental Medicine (Pediatrics) D-5, Osaka University Graduate School of Medicine, Suita City, Osaka, Japan.
Kondo
T
T
Nakagawa
N
N
Inagi
R
R
Miyoshi
H
H
Sunagawa
T
T
Okada
S
S
Yamanishi
K
K
D015139:Blotting, Southern; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000097:blood / Q000821:virology*; D015654:Herpesvirus 6, Human / Q000254:growth & development / Q000502:physiology*; D016199:Herpesvirus 7, Human / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000821:virology; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D014775:Virus Activation
D004279:DNA, Viral