The role of RXR-alpha in retinoic acid-induced cleft palate as assessed with the RXR-alpha knockout mouse.
Treatment of pregnant mice with retinoic acid (RA) in mid-gestation produces cleft palate and limb defects in the fetuses. RXR-alpha has been previously shown to mediate the teratogenic effects of RA in the limb. In this study, we show that RXR-alpha is also involved in retinoid-induced palatal clefting. Treatment of RXR-alpha knockout mice with a teratogenic dose of RA on gestation day 11 or 12 induces cleft palate at a lower frequency than that seen in wild-type animals.
Nugent
P
P
Department of Molecular, Cellular and Craniofacial Biology, University of Louisville School of Dentistry and University of Louisville Birth Defects Center, KY 40292, USA. pfnuge01@gwise.louisville.edu
Sucov
H M
HM
Pisano
M M
MM
Greene
R M
RM
D000818:Animals; D002972:Cleft Palate / Q000139:chemically induced* / Q000378:metabolism; D005333:Fetus / Q000002:abnormalities / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D018168:Receptors, Retinoic Acid / Q000378:metabolism*; D047488:Retinoid X Receptors; D013723:Teratogens / Q000633:toxicity*; D014157:Transcription Factors / Q000378:metabolism*; D014212:Tretinoin / Q000633:toxicity*
D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D013723:Teratogens; D014157:Transcription Factors; D014212:Tretinoin