Comparative blood coagulation studies in the ostrich.
Blood coagulation of the ostrich was compared to that of mammalian (man and sheep), avian (chicken) and reptilian (puff adder) systems. The international normalised ratio (INR), partial thromboplastin time (PTT), thrombin time and fibrin degradation were determined, as well as the various coagulation factors in venous ostrich plasma, using human physiological substrates. Thromboplastin was isolated from fresh brain tissue with the exception of the reptile for which lung tissue was used. The levels of markers of the coagulation [antithrombin III (AT), factor X (FX) and prothrombin], the fibrinolytic (alpha2-antiplasmin) and the kallikrein system were determined using chromogenic substrates. Elevated values for INR, PTT and thrombin time were obtained as compared to known human standards. It was found that factors VII, IX, X, XI and XII were absent from ostrich plasma. A study of the homologous and heterologous thromboplastin activities indicated that ostrich plasma exhibited a lower thromboplastic activity when compared to human standards, but was comparable to avian and reptilian values. Ostrich plasma revealed 42.2% FX, 72.9% AT, 35.3% prothrombin, 115.6% alpha2-antiplasmin and 19.8% plasma kallikrein, relative to human plasma. All the results suggest that the ostrich coagulatory system has not evolved to include all the complex myriad of reactions found in the human system.
Frost
C L
CL
Department of Biochemistry and Microbiology, University of Port Elizabeth, South Africa.
Naudé
R J
RJ
Oelofsen
W
W
Jacobson
B
B
D000818:Animals; D015415:Biomarkers; D001779:Blood Coagulation Factors / Q000502:physiology*; D002645:Chickens; D005342:Fibrinolysis / Q000502:physiology; D006801:Humans; D016234:Kallikrein-Kinin System / Q000502:physiology; D012756:Sheep; D013045:Species Specificity; D020310:Struthioniformes / Q000097:blood* / Q000502:physiology; D013925:Thromboplastin / Q000502:physiology; D017819:Viperidae
D015415:Biomarkers; D001779:Blood Coagulation Factors; D013925:Thromboplastin