[Heme oxgenase-1 gene induction and the mechanism in the rat vasospasm model].
Using fluorescent differential display and quantitative reverse-PCR, we found in the rat vasospasm model that heme oxygenase-1 messenger RNA was induced in basilar artery. Intracistemal injection of antisense OH-1 oligodeoxynucleotide significantly reduced HO-1 mRNA and HO-1 protein levels and enhanced angiographic vasospasm. Thus, we demonstrate that HO-1 induction may play a important role in the resolution of delayed vasospasm after subarachnoid hemorrhage.
Tsunoda
H
H
Department of Molecular and Cellar Pharmacology, Mie University, School of Medicine, Japan.
Suzuki
H
H
Kanamaru
K
K
Tanaka
T
T
D000818:Animals; D016373:DNA, Antisense; D004195:Disease Models, Animal; D015870:Gene Expression; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis / Q000235:genetics*; D051547:Heme Oxygenase-1; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013345:Subarachnoid Hemorrhage / Q000503:physiopathology; D020301:Vasospasm, Intracranial / Q000503:physiopathology*
D016373:DNA, Antisense; D012333:RNA, Messenger; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1