title: Differential transport of DNA by a rectified Brownian motion device.
abstract: An interdigitated electrode array (IDEA) device has been designed and used to transport DNA based on a Brownian ratchet mechanism. This migration is produced by the periodic formation of an asymmetric sawtooth electric field in the device. Oligonucleotides of 25, 50, and 100 bases in length were tested using two different array geometries. DNA transport as a function of DNA size, electric field frequency, and array geometry is shown to be in qualitative agreement with theory. Such a device could provide for DNA separations over a broad size range, and can be readily scaled as a component in a microfabricated DNA analysis system.
authors.affiliation: CuraGen, New Haven, CT 06511, USA. rhammond@curagen.com
authors.forename: R W
authors.initials: RW
authors.lastname: Hammond
authors.forename: J S
authors.initials: JS
authors.lastname: Bader
authors.forename: S A
authors.initials: SA
authors.lastname: Henck
authors.forename: M W
authors.initials: MW
authors.lastname: Deem
authors.forename: G A
authors.initials: GA
authors.lastname: McDermott
authors.forename: J M
authors.initials: JM
authors.lastname: Bustillo
authors.forename: J M
authors.initials: JM
authors.lastname: Rothberg
mesh_terms: D000818:Animals; D002627:Chemistry, Physical / Q000295:instrumentation / Q000379:methods; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004058:Diffusion; D004586:Electrophoresis / Q000379:methods; D004864:Equipment and Supplies; D006801:Humans
chemical_list: D004247:DNA