title: Multiple lethal effects induced by a benzimidazole anthelmintic in the anterior intestine of the nematode Haemonchus contortus.
abstract: A mechanism of benzimidazole efficacy against parasitic nematodes is postulated to involve inhibition of intestinal secretory vesicle transport via depolymerization of microtubules. We show that fenbendazole (FBZ) treatment of lambs causes pathology localized to the anterior intestine in the parasitic nematode Haemonchus contortus. The pathology included gross disintegration of the anterior intestine, DNA fragmentation in anterior intestinal nuclei with characteristics of an apoptosis-like process, and inhibition of host erythrocyte digestion. These lethal effects were associated with inhibited transport of apical secretory vesicles in the anterior intestine, and then generalized dispersal of these vesicles contents throughout the intestinal cytoplasm and worm body. Cytoplasmic accumulation of secretory vesicles and undigested erythrocytes preceded DNA fragmentation and vesicle content dispersal. Both DNA fragmentation and vesicle content dispersal were detected in disintegrated intestine and intestine that had not yet undergone disintegration. These pathologic effects in the anterior intestine appear sufficient to explain the efficacy of FBZ against adult H. contortus. The results implicate mechanisms in the anterior intestine that govern dispersal of apical secretory vesicle contents, DNA fragmentation and tissue disintegration as effectors of this pathology.
authors.affiliation: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA. djasmer@vetmed.wsu.edu
authors.forename: D P
authors.initials: DP
authors.lastname: Jasmer
authors.forename: C
authors.initials: C
authors.lastname: Yao
authors.forename: A
authors.initials: A
authors.lastname: Rehman
authors.forename: S
authors.initials: S
authors.lastname: Johnson
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D017209:Apoptosis; D002467:Cell Nucleus / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004912:Erythrocytes / Q000378:metabolism; D005273:Fenbendazole / Q000494:pharmacology*; D006188:Haemonchiasis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000473:pathology; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D000969:Antinematodal Agents; D014404:Tubulin; D005273:Fenbendazole