Increased serum levels of soluble L-selectin (CD62L) in patients with active systemic lupus erythematosus (SLE).
The adhesion molecule L-selectin (CD62L) mediates lymphocyte recirculation and leucocyte rolling on vascular endothelium at sites of inflammation. Serum levels of soluble L-selectin (sL-selectin) were measured in patients with SLE in order to relate these levels to clinical activity and immunological parameters. An ELISA was used to detect the soluble form of human L-selectin (CD62L) in 42 patients with SLE and in 33 healthy individuals. The mean +/- s.e.m. values of sL-selectin were 1285 +/- 121 ng/ml for patients with SLE and 986 +/- 180 ng/ml for healthy blood donors, but there was no significant difference. When patients with active SLE were analysed, higher levels of circulating sL-selectin were found when compared with patients without activity (1497 +/- 167 ng/ml versus 941 +/- 150 ng/ml; P = 0.028). We found a significant correlation between the levels of sL-selectin and of dsDNA antibodies (r = 0.36, P = 0. 044) and between levels of sL-selectin and SLE Disease Activity Index (SLEDAI) score (r = 0.42, P = 0.003). Patients with active SLE studied cross-sectionally showed significant elevations of sL-selectin (CD62L) compared with controls. Thus, the levels of this soluble adhesion molecule correlated with active disease and levels of anti-dsDNA antibodies.
Font
J
J
Systemic Autoimmune Diseases Unit, Department of Cellular Biology, University of Barcelona, Spain. font@medicina.ub.es
Pizcueta
P
P
Ramos-Casals
M
M
Cervera
R
R
García-Carrasco
M
M
Navarro
M
M
Ingelmo
M
M
Engel
P
P
D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D003430:Cross-Sectional Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D019041:L-Selectin / Q000097:blood*; D008180:Lupus Erythematosus, Systemic / Q000209:etiology / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012995:Solubility; D013997:Time Factors
D000974:Antibodies, Antinuclear; D019041:L-Selectin