A new and rapid method for the determination of nicarbazin residues in poultry feed, eggs and muscle tissue using supercritical fluid extraction and high performance liquid chromatography.
A simple and rapid method, using supercritical fluid extraction (SFE) with off-line high-performance liquid chromatography for the isolation and determination of nicarbazin, a popular broad-spectrum coccidiostatic drug used principally in poultry, is described. Results show good repeatability with a minimum quantification level of 0.4 microgram g-1 and mean 'spiked' recoveries of 98%, 100% and 99% using poultry feeds (n = 18), eggs (n = 28) and chicken tissue (n = 20), respectively. SFE using carbon dioxide is proposed as an alternative isolation method to the current Association of Official Analytical Chemists (AOAC) procedure which involves the use of large volumes of a harmful solvent (dimethylformamide) and requires a long tedious separation and clean-up regime (6 h) prior to its determination.
Matabudul
D K
DK
Food Research Centre, School of Applied Science, South Bank University, London, UK.
Crosby
N T
NT
Sumar
S
S
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination; D008460:Meat / Q000032:analysis*; D009528:Nicarbazin / Q000032:analysis*
D003049:Coccidiostats; D009528:Nicarbazin