title: Purification and characterization of an iron superoxide dismutase and a catalase from the sulfate-reducing bacterium Desulfovibrio gigas.
abstract: The iron-containing superoxide dismutase (FeSOD; EC 1.15.1.1) and catalase (EC 1.11.1.6) enzymes constitutively expressed by the strictly anaerobic bacterium Desulfovibrio gigas were purified and characterized. The FeSOD, isolated as a homodimer of 22-kDa subunits, has a specific activity of 1,900 U/mg and exhibits an electron paramagnetic resonance (EPR) spectrum characteristic of high-spin ferric iron in a rhombically distorted ligand field. Like other FeSODs from different organisms, D. gigas FeSOD is sensitive to H(2)O(2) and azide but not to cyanide. The N-terminal amino acid sequence shows a high degree of homology with other SODs from different sources. On the other hand, D. gigas catalase has an estimated molecular mass of 186 +/- 8 kDa, consisting of three subunits of 61 kDa, and shows no peroxidase activity. This enzyme is very sensitive to H(2)O(2) and cyanide and only slightly sensitive to sulfide. The native enzyme contains one heme per molecule and exhibits a characteristic high-spin ferric-heme EPR spectrum (g(y,x) = 6.4, 5.4); it has a specific activity of 4,200 U/mg, which is unusually low for this class of enzyme. The importance of these two enzymes in the context of oxygen utilization by this anaerobic organism is discussed.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780 Oeiras, Portugal. Wagner@itqb.unl.pt
authors.forename: W G
authors.initials: WG
authors.lastname: Dos Santos
authors.forename: I
authors.initials: I
authors.lastname: Pacheco
authors.forename: M Y
authors.initials: MY
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Teixeira
authors.forename: A V
authors.initials: AV
authors.lastname: Xavier
authors.forename: J
authors.initials: J
authors.lastname: LeGall
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000693:Anaerobiosis; D002374:Catalase / Q000302:isolation & purification*; D003901:Desulfovibrio / Q000201:enzymology* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D008280:Magnetics; D008969:Molecular Sequence Data; D013482:Superoxide Dismutase / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D002374:Catalase; D013482:Superoxide Dismutase