Effect of carbon-coated polyethylene terephthalate on prostacyclin release by endothelial cells stimulated with arachidonic acid in vitro.
The production of 6-keto prostaglandin F1alpha (6-keto-PGF1alpha), stable metabolyte of prostacyclin, by cultured human endothelial cells in contact with carbon- and collagen-coated polyethylene terephthalate (PC), was assessed by enzyme immunoassay. As control material, tissue culture-treated polystyrene was used. The cultures were put in contact with the materials for 48 h and then were stimulated with 0.1 mM arachidonic acid for 3 h. The stimulation induced a highly significant increase of 6-keto-PGF1alpha in the cultures in contact with the control material. PC induced only insignificant variations in stimulated cultures compared to unstimulated ones. In conclusion, PC determined a decrease in the endothelial cell response to stimulation with arachidonic acid.
Cenni
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E
Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
Corradini
A
A
Di Leo
A
A
Montanaro
L
L
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D016718:Arachidonic Acid / Q000494:pharmacology*; D015415:Biomarkers; D001807:Blood Vessel Prosthesis; D002244:Carbon / Q000737:chemistry*; D002478:Cells, Cultured / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D008422:Materials Testing; D011093:Polyethylene Terephthalates / Q000494:pharmacology*; D014471:Umbilical Veins
D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D011093:Polyethylene Terephthalates; D016718:Arachidonic Acid; D015121:6-Ketoprostaglandin F1 alpha; D002244:Carbon; D003094:Collagen; D011464:Epoprostenol