Selective photocleavage of DNA and RNA by anthraquinone derivatives: targeting the single-strand region of hairpin structures.
A tetracationic anthraquinone derivative (27AQS2) binds to hairpin DNA and RNA. Ultraviolet irradiation of the bound quinone causes cleavage in the loop region of both oligonucleotides and at guanines in the stem region of the DNA hairpin. The absence of observable strand cleavage at guanines in the RNA hairpin suggests that either aniline treatment does not cause cleavage at damaged guanines in RNA or that radical cation migration does not occur readily in RNA duplexes. The ability to target the single-stranded regions of DNA and RNA structures is an important property of this photonuclease.
Fernandez-Saiz
M
M
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
Henderson
P T
PT
Wilson
W D
WD
Schuster
G B
GB
D000880:Anthraquinones / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010782:Photolysis; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D000880:Anthraquinones; D004277:DNA, Single-Stranded; D007364:Intercalating Agents; C111123:N,N,N',N'-tetrakis(3-aminopropyl)-2,7-anthraquinonesulfonamide tetrahydrochloride; D017319:Photosensitizing Agents; D012313:RNA