title: Effect of membrane lipid composition on the conformational equilibria of the nicotinic acetylcholine receptor.
abstract: The effects of cholesterol (Chol) and an anionic lipid, dioleoylphosphatidic acid (DOPA) on the conformational equilibria of the nicotinic acetylcholine receptor (nAChR) have been investigated using Fourier transform infrared difference spectroscopy. The difference between spectra recorded in the presence and absence of agonist from the nAChR reconstituted into 3:1:1 egg phosphatidylcholine (EPC)/DOPA/Chol membranes exhibits positive and negative bands that serve as markers of the structural changes associated with the resting to desensitized conformational change. These markers are absent in similar difference spectra recorded from the nAChR reconstituted into EPC membranes lacking both Chol and DOPA, indicating that the nAChR cannot undergo conformational change in response to agonist binding. When low levels of either Chol or DOPA up to 25 mol % of the total lipid are included in the EPC membranes, the markers suggest the predominant stabilization of a conformation that is a structural intermediate between the resting and desensitized states. At higher levels of either Chol or DOPA, the nAChR is stabilized in a conformation that is capable of undergoing agonist-induced desensitization, although DOPA appears to be required for the nAChR to adopt a conformation fully equivalent to that found in native and 3:1:1 EPC/DOPA/Chol membranes. The ability of these two structurally diverse lipids, as well as others (Ryan, S. E., Demers, C. N., Chew, J. P., Baenziger, J. E. (1996) J. Biol. Chem. 271, 24590-24597), to modulate the functional state of the nAChR suggests that lipids act on the nAChR via an indirect effect on some physical property of the lipid bilayer. The data also suggest that anionic lipids are essential to stabilize a fully functional nAChR. We propose that membrane fluidity modulates the relative populations of nAChRs in the resting and desensitized states but that subtle structural changes in the presence of anionic lipids are essential for full activity.
authors.affiliation: Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada. jebaenz@uottawa.ca
authors.forename: J E
authors.initials: JE
authors.lastname: Baenziger
authors.forename: M L
authors.initials: ML
authors.lastname: Morris
authors.forename: T E
authors.initials: TE
authors.lastname: Darsaut
authors.forename: S E
authors.initials: SE
authors.lastname: Ryan
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000737:chemistry / Q000494:pharmacology*; D004557:Electric Organ / Q000378:metabolism; D007700:Kinetics; D008563:Membrane Lipids / Q000737:chemistry / Q000494:pharmacology*; D008567:Membranes, Artificial; D008958:Models, Molecular; D010712:Phosphatidic Acids / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014101:Torpedo
chemical_list: D008563:Membrane Lipids; D008567:Membranes, Artificial; D010712:Phosphatidic Acids; D011978:Receptors, Nicotinic; C037657:dioleoylphosphatidic acid; D002784:Cholesterol