Molecular cloning, expression and immunological characterisation of Lol p 5C, a novel allergen isoform of rye grass pollen demonstrating high IgE reactivity.
A novel isoform of a major rye grass pollen allergen Lol p 5 was isolated from a cDNA expression library. The new isoform, Lol p 5C, shares 95% amino acid sequence identity with Lol p 5A. Both isoforms demonstrated shared antigenic activity but different allergenic activities. Recombinant Lol p 5C demonstrated 100% IgE reactivity in 22 rye grass pollen sensitive patients. In comparison, recombinant Lol p 5A showed IgE reactivity in less than 64% of the patients. Therefore, Lol p 5C represents a novel and highly IgE-reactive isoform allergen of rye grass pollen.
Suphioglu
C
C
Department of Allergy, Asthma and Clinical Immunology, Monash University Medical School, Alfred Hospital, Prahran, Vic., Australia. cenk.suphioglu@med.monash.edu.au
Mawdsley
D
D
Schäppi
G
G
Gruehn
S
S
de Leon
M
M
Rolland
J M
JM
O'Hehir
R E
RE
D000485:Allergens; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism; D052179:Antigens, Plant; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D015723:Gene Library; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000378:metabolism*; D008129:Lolium / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D018076:DNA, Complementary; C084941:Lol p V protein, Lolium perenne; D010940:Plant Proteins; D020033:Protein Isoforms; D007073:Immunoglobulin E