Development of the myoglobin sensor based on the surface plasmon resonance.
Bioaffinic sensor based on the surface plasmon resonance (SPR) with golden layer as a transducer was developed. This sensor was applied for control of antigen-antibodies (Ag-Ab) interactions. For modification of transducer surface the spontaneously organized molecular assemblies of two types of mercaptan were formed. It was shown that the created bioaffinic sensor allowed to detect minimal concentration of antibodies, about 1 ng/ml, to the human heart myoglobin, which preliminary was immobilized on the sensitive surface. The particularities of the dynamics of protein interaction were observed. Duration of incubation with the biospecific component was no more than 5 minutes. After rupturing of specific binding it was possible to obtain initial signal again during 7-9 cycles.
Starodub
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A.V. Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv.
Dibrova
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TL
Shirshov YuM
Kostyukevych
K V
KV
D015374:Biosensing Techniques; D006801:Humans; D009206:Myocardium / Q000737:chemistry; D009211:Myoglobin / Q000032:analysis*; D020349:Surface Plasmon Resonance
D009211:Myoglobin