Comparison of animal infectivity and nucleic acid staining for assessment of Cryptosporidium parvum viability in water.
Cryptosporidium parvum oocysts were stained with the fluorogenic dyes SYTO-9 and SYTO-59 and sorted by flow cytometry in order to determine whether the fluorescent staining intensity correlated with the ability of oocysts to infect neonatal CD-1 mice. Oocysts that did not fluoresce or that displayed weak fluorescent intensity when stained with SYTO-9 or SYTO-59 readily established infections in mice, whereas those oocysts that fluoresced brightly did not. Although fluorescent staining profiles varied among different batches of oocysts, a relative cutoff in fluorescent staining intensity that correlated with animal infectivity was observed for all batches.
Neumann
N F
NF
Department of Biological Sciences, University of Alberta, Edmonton, Canada.
Gyürek
L L
LL
Gammie
L
L
Finch
G R
GR
Belosevic
M
M
D000818:Animals; D004396:Coloring Agents / Q000378:metabolism; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D051379:Mice; D018613:Microscopy, Confocal; D009696:Nucleic Acids / Q000032:analysis*; D009930:Organic Chemicals; D013194:Staining and Labeling; D014881:Water Supply
D004396:Coloring Agents; D009696:Nucleic Acids; D009930:Organic Chemicals; C103389:SYTO 9