title: Sequence variations within PrfA DNA binding sites and effects on Listeria monocytogenes virulence gene expression.
abstract: Reporter gene fusions were used to investigate the contributions of PrfA DNA binding sites to Listeria monocytogenes virulence gene expression. Our results suggest that the DNA sequence of PrfA binding sites determines the levels of expression of certain virulence genes, such as hly and mpl. Other virulence genes, such as actA and plcB, may depend upon additional factors for full regulation of gene expression.
authors.affiliation: Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan.
authors.forename: J R
authors.initials: JR
authors.lastname: Williams
authors.forename: C
authors.initials: C
authors.lastname: Thayyullathil
authors.forename: N E
authors.initials: NE
authors.lastname: Freitag
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008089:Listeria monocytogenes / Q000378:metabolism / Q000472:pathogenicity*; D008565:Membrane Proteins / Q000235:genetics; D008666:Metalloendopeptidases / Q000235:genetics; D009154:Mutation; D010454:Peptide Termination Factors; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D010454:Peptide Termination Factors; D015534:Trans-Activators; C072980:actA protein, Listeria monocytogenes; D008666:Metalloendopeptidases; C080367:mpl protein, Listeria monocytogenes