Adjuvant effect of a 14-member macrolide antibiotic on DNA vaccine.
Macrolide antibiotics have unique immunomodulatory actions apart from their antimicrobial properties. We examined the effect of erythromycin (EM), a 14-member macrolide, on the immune response to a DNA vaccine that induces a T-helper-1 (Th1)-biased immune response through a Th1-promoting adjuvant effect of unmethylated CpG motifs within plasmid DNA. EM enhanced Th1 responses in plasmid DNA-immunized mice as measured by antigen-specific IgG2a antibody production, interferon-gamma production by antigen-specific CD4(+) T cells, and cytotoxic T lymphocyte responses. EM augmented the accessory cell activity of unmethylated CpG DNA-stimulated antigen-presenting cells (APCs), suggesting that EM enhances Th1 responses to a DNA vaccine, possibly through augmentation of accessory cell activity of APCs stimulated with CpG motifs within plasmid DNA.
Sato
Y
Y
Department of Internal Medicine II, Fukushima Medical University School of Medicine, Fukushima, 960-1295, Japan. med2fmc@po.sphere.ne.jp
Shishido
H
H
Kobayashi
H
H
Takeda
J
J
Irisawa
A
A
Miyata
M
M
Nishimaki
T
T
Fujita
T
T
Kasukawa
R
R
D000276:Adjuvants, Immunologic; D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology* / Q000494:pharmacology; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology; D018899:CpG Islands; D004917:Erythromycin / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D015570:Josamycin / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D001616:beta-Galactosidase / Q000235:genetics
D000276:Adjuvants, Immunologic; D000900:Anti-Bacterial Agents; D019444:Vaccines, DNA; D004917:Erythromycin; D001616:beta-Galactosidase; D015570:Josamycin