title: Chromosomal beta-lactamase is packaged into membrane vesicles and secreted from Pseudomonas aeruginosa.
abstract: Membrane vesicles were isolated from one beta-lactam-sensitive and three beta-lactam-resistant Pseudomonas aeruginosa clinical isolates from patients with cystic fibrosis. The presence of the chromosomally encoded beta-lactamase in the membrane vesicles was shown by electron microscopy and enzymatic studies. This is the first report of extracellular secretion of beta-lactamase in P. aeruginosa and it seems that the enzyme is packaged into membrane vesicles.
authors.affiliation: Institute for Medical Microbiology and Immunology, Panum Inst. 24.1, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark. O.Ciofu@immi.ku.dk
authors.forename: O
authors.initials: O
authors.lastname: Ciofu
authors.forename: T J
authors.initials: TJ
authors.lastname: Beveridge
authors.forename: J
authors.initials: J
authors.lastname: Kadurugamuwa
authors.forename: J
authors.initials: J
authors.lastname: Walther-Rasmussen
authors.forename: N
authors.initials: N
authors.lastname: Høiby
mesh_terms: D002462:Cell Membrane / Q000201:enzymology; D002876:Chromosomes, Bacterial / Q000235:genetics; D003550:Cystic Fibrosis / Q000382:microbiology; D006801:Humans; D008168:Lung / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases