Förster excitation energy transfer in peridinin-chlorophyll-a-protein.
Time-resolved fluorescence anisotropy spectroscopy has been used to study the chlorophyll a (Chl a) to Chl a excitation energy transfer in the water-soluble peridinin-chlorophyll a-protein (PCP) of the dinoflagellate Amphidinium carterae. Monomeric PCP binds eight peridinins and two Chl a. The trimeric structure of PCP, resolved at 2 A (, Science. 272:1788-1791), allows accurate calculations of energy transfer times by use of the Förster equation. The anisotropy decay time constants of 6.8 +/- 0.8 ps (tau(1)) and 350 +/- 15 ps (tau(2)) are respectively assigned to intra- and intermonomeric excitation equilibration times. Using the ratio tau(1)/tau(2) and the amplitude of the anisotropy, the best fit of the experimental data is achieved when the Q(y) transition dipole moment is rotated by 2-7 degrees with respect to the y axis in the plane of the Chl a molecule. In contrast to the conclusion of, Biochemistry. 23:1564-1571) that the refractive index (n) in the Förster equation should be equal to that of the solvent, n can be estimated to be 1.6 +/- 0.1, which is larger than that of the solvent (water). Based on our observations we predict that the relatively slow intermonomeric energy transfer in vivo is overruled by faster energy transfer from a PCP monomer to, e.g., the light-harvesting a/c complex.
Kleima
F J
FJ
Faculty of Sciences, Division of Physics, Vrije Universiteit, 1081 HV Amsterdam, the Netherlands. foske@nat.vu.nl
Hofmann
E
E
Gobets
B
B
van Stokkum
I H
IH
van Grondelle
R
R
Diederichs
K
K
van Amerongen
H
H
D002338:Carotenoids / Q000737:chemistry* / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D004735:Energy Transfer; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes; D007700:Kinetics; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D015800:Protozoan Proteins / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors
D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D015800:Protozoan Proteins; C073227:peridinin chlorophyll-a protein, Dinophyceae; D002734:Chlorophyll; D002338:Carotenoids