title: Sheep pulmonary adenomatosis: characterization of two pathological forms associated with jaagsiekte retrovirus.
abstract: Pathological and immunohistochemical studies were performed on the lungs of 10 sheep with lesions of "classical" sheep pulmonary adenomatosis (SPA) and six sheep with "atypical" lung tumours. Lung tumour samples and other tissues from the same 16 animals were tested for the presence of jaagsiekte retrovirus (JSRV) by a polymerase chain reaction (PCR) that amplified a portion of the U3 long terminal repeat. The differences in the gross appearance of the classical and atypical forms paralleled the histopathological differences. The latter mainly concerned the stroma of the tumours which in the atypical cases was more heavily infiltrated by inflammatory cells and connective tissue fibres. JSRV major capsid protein was detected immunohistochemically in the epithelial transformed cells of both classical and atypical tumours, but the immune reactivity was slightly milder in atypical SPA. Proviral U3 sequences of JSRV were detected by specific PCR in all the tumour samples. Furthermore, the sequences of amplimers obtained from the two different pathological forms of the tumour were very similar. However, the dissemination of JSRV to other organs was greater in sheep with classical SPA than in those with atypical SPA. The pathological and virological features of these two forms of tumour are compared in an attempt to clarify whether classical and atypical SPA are two separate diseases or different expressions of a single disease spectrum.
authors.affiliation: Departamento de Patología Animal, NEIKER A. B, Derio, 48160, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Goti
authors.forename: L
authors.initials: L
authors.lastname: González
authors.forename: C
authors.initials: C
authors.lastname: Cousens
authors.forename: N
authors.initials: N
authors.lastname: Cortabarría
authors.forename: A B
authors.initials: AB
authors.lastname: Extramiana
authors.forename: E
authors.initials: E
authors.lastname: Minguijón
authors.forename: A
authors.initials: A
authors.lastname: Ortín
authors.forename: M
authors.initials: M
authors.lastname: De las Heras
authors.forename: J M
authors.initials: JM
authors.lastname: Sharp
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D030121:Betaretrovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002213:Capsid / Q000235:genetics / Q000276:immunology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008175:Lung Neoplasms / Q000276:immunology / Q000473:pathology / Q000662:veterinary / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011648:Pulmonary Adenomatosis, Ovine / Q000276:immunology / Q000473:pathology* / Q000821:virology; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000473:pathology / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004273:DNA, Neoplasm; D012191:Retroviridae Proteins