title: Coculture system to assess biocompatibility of candidate orthopaedic materials.
abstract: When examining the biocompatibility of materials for orthopaedic use, it is important to examine the in vitro response of osteoblasts and macrophages to the material. The purpose of the current study was to develop an in vitro system that more accurately models the dynamic macrophage and osteoblast interactive response to biomaterials. A filter coculture system was designed that enables two cell types to be separated, while permitting cellular mediators to diffuse through its porous membrane. Using the filter coculture system, macrophage and osteoblast interaction in response to polymethylmethacrylate particle exposure was examined. As a marker of bone resorption potential, the level of prostaglandin E2 released from the macrophage and osteoblast coculture was compared with traditionally used in vitro culture systems. Cells interacting in the filter coculture system were found to produce prostaglandin E2 levels significantly greater than those of traditional conditioned media transfer systems. This filter coculture system offers an in vitro model that accounts for the continual cell to cell mediator interaction of two cell types simultaneously exposed to an implant material. This system may be useful in examining the biocompatibility of candidate materials at the bone interface, and thus elucidating the mechanisms of material induced bone resorption.
authors.affiliation: Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Attawia
authors.forename: J J
authors.initials: JJ
authors.lastname: Nicholson
authors.forename: C T
authors.initials: CT
authors.lastname: Laurencin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D001862:Bone Resorption / Q000378:metabolism; D002450:Cell Communication; D018929:Cell Culture Techniques; D002460:Cell Line; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D015232:Dinoprostone / Q000032:analysis; D005374:Filtration / Q000295:instrumentation; D008264:Macrophages / Q000166:cytology*; D008422:Materials Testing / Q000379:methods*; D008567:Membranes, Artificial; D051379:Mice; D009983:Orthopedic Equipment; D010006:Osteoblasts / Q000166:cytology*; D010316:Particle Size; D019904:Polymethyl Methacrylate / Q000737:chemistry; D016062:Porosity
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D017077:Culture Media, Conditioned; D008567:Membranes, Artificial; D019904:Polymethyl Methacrylate; D015232:Dinoprostone