Three dimensional (3D) analysis of the morphological changes induced by 50 Hz magnetic field exposure on human lymphoblastoid cells (Raji).
Human Raji B lymphoid cells after exposure for 64 h to a 1 mT (rms) 50 Hz sinusoidal magnetic field showed a reorganization of membrane and cytoskeletal components. Atomic force microscopy in air revealed several modifications in 80% of the exposed cells, such as loss of microvilli-like structures followed by progressive appearance of membrane introflections. This change in plasma membrane morphology was also accompanied by a different actin distribution, as detected by phalloidin fluorescence. These observations support our previous hypothesis that electric and magnetic fields may modify the plasma membrane structure.
Lisi
A
A
Istituto di Medicina Sperimentale - CNR, Via Fosso del Cavaliere, Roma, Italy.
Pozzi
D
D
Pasquali
E
E
Rieti
S
S
Girasole
M
M
Cricenti
A
A
Generosi
R
R
Serafino
A L
AL
Congiu-Castellano
A
A
Ravagnan
G
G
Giuliani
L
L
Grimaldi
S
S
D001402:B-Lymphocytes / Q000473:pathology / Q000528:radiation effects* / Q000648:ultrastructure; D002051:Burkitt Lymphoma; D002462:Cell Membrane / Q000528:radiation effects* / Q000648:ultrastructure; D003599:Cytoskeleton / Q000528:radiation effects* / Q000648:ultrastructure; D004574:Electromagnetic Fields; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D014407:Tumor Cells, Cultured