title: An aerotaxis transducer gene from Pseudomonas putida.
abstract: An aerotaxis gene, aer, was cloned from Pseudomonas putida PRS2000. A P. putida aer mutant displayed an altered aerotactic response in a capillary assay. Wild-type P. putida clustered at the air/liquid interface. In contrast, the aer mutant did not cluster at the interface, but instead formed a diffuse band at a distance from the meniscus. Wild-type aer, provided in trans, complemented the aer mutant to an aerotactic response that was stronger than wild-type. The P. putida Aer sequence is similar over its entire length to the aerotaxis (energy taxis) signal transducer protein, Aer, of Escherichia coli. The amino-terminus is similar to redox-sensing regulatory proteins, and the carboxy-terminus contains the highly conserved domain present in chemotactic transducers.
authors.affiliation: Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA.
authors.forename: N N
authors.initials: NN
authors.lastname: Nichols
authors.forename: C S
authors.initials: CS
authors.lastname: Harwood
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002633:Chemotaxis / Q000235:genetics; D003001:Cloning, Molecular; D003470:Culture Media; D004734:Energy Metabolism / Q000502:physiology; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D036341:Intercellular Signaling Peptides and Proteins; D007613:Kanamycin Resistance / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016958:Pseudomonas putida / Q000235:genetics* / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics; D019802:Succinic Acid / Q000378:metabolism
chemical_list: C108679:Aer protein, E coli; D002352:Carrier Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D036341:Intercellular Signaling Peptides and Proteins; D019802:Succinic Acid