title: Thermodynamic features of the chemical and thermal denaturations of human serum albumin.
abstract: The unfolding process of human serum albumin between pH 5.4 and 9.9 was studied by chemical and thermal denaturations. The experimental results showed that there is no correlation between the stability of albumin at different pH values determined by both methods. The free energy change of unfolding versus concentration of guanidine showed a close dependence on the pH, suggesting that the variation of the electrical charge of albumin influences the final state of the unfolded form of the protein. Spectroscopic techniques, such as native fluorescence of the protein and circular dichroism, demonstrated that the unfolded state of the protein obtained from both methods possesses a different helical content. The solvophobic effect and the entropy of the chains have no influence on the final unfolding state when the protein is unfolded by thermal treatment, while, when the protein is unfolded by chemical denaturants, both effects depend on the medium pH. The results indicate that guanidine and urea interact with albumin by electrostatic forces, yielding a randomly coiled conformation in its unfolded state, while thermal denaturation produces a molten globule state and the aggregation of the protein; therefore, both methods yield different structurally unfolded states of the albumin.
authors.affiliation: Departamento de Química-Fisica, Facultad de Ciencias Bioquímicas y Farmacéuticas, CIUNR and CONICET, Universidad Nacional de Rosario, Argentina.
authors.forename: B
authors.initials: B
authors.lastname: Farruggia
authors.forename: G A
authors.initials: GA
authors.lastname: Picó
mesh_terms: D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D019791:Guanidine; D006358:Hot Temperature; D006801:Humans; D011489:Protein Denaturation; D017510:Protein Folding; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014508:Urea
chemical_list: D012709:Serum Albumin; D014508:Urea; D019791:Guanidine