Murine response to DNA encoding herpes simplex virus type-1 glycoprotein D targeted to the liver.
Plasmid DNA encoding herpes simplex virus type-1 glycoprotein D (gD-1) was complexed with asialoorosomucoid conjugated to poly-L-lysine. Following its intravenous injection into BALB/c mice, this complex was targeted to the liver. Liver cells expressing gD-1 were detected immunohistochemically through day 6 post-immunization, while gD-1 DNA was detectable through 14 days post-immunization. Decline of gD-1 expression and detectable gD-1 DNA in the liver correlated with influx of T cells, predominantly CD4(+). The ASOR-poly-L-lysine DNA carrier system promotes hepatic expression of gD-1 and may be useful in vaccination against herpes simplex virus type-1.
Rogers
J V
JV
Biomedical Sciences Ph.D. Program, Wright State University, Dayton, OH 45435, USA.
Hull
B E
BE
Fink
P S
PS
Chiou
H C
HC
Bigley
N J
NJ
D000818:Animals; D001212:Asialoglycoproteins / Q000008:administration & dosage; D005260:Female; D018259:Herpesvirus 1, Human / Q000276:immunology*; D007114:Immunization; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009961:Orosomucoid / Q000008:administration & dosage / Q000031:analogs & derivatives; D011107:Polylysine / Q000008:administration & dosage; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D001212:Asialoglycoproteins; D009961:Orosomucoid; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C017030:asialoorosomucoid; C039447:glycoprotein D, Human herpesvirus 1; D011107:Polylysine