title: Age-related changes in the kinetics of water transport in normal human lenses.
abstract: Magnetic resonance microscopy (MRM) has been used to study the kinetics of water transport in human eye lenses. Fresh lenses obtained from the Queensland Eye Bank were incubated at 34.5 degrees C in artificial aqueous humour (AAH) containing nutrients and metabolites similar to those that are present in vivo. MR images were acquired over approximately a 20 hr period following replacement of H(2)O based AAH with deuterium oxide (D(2)O) based AAH. NMR signal intensity from the lenses decreased with time corresponding to a decrease in concentration of H(2)O within the lenses. A statistically significant correlation (P<0.001) was found between the rate of NMR signal loss from the lens nuclei and increasing age of the lenses. The results show that as lenses age, there is a reduction in the rate at which water and presumably also water soluble low molecular weight metabolites, can enter the cells of the lens nucleus via the epithelium and cortex. A decrease in the rate of transport of water, nutrients and anti-oxidants (e.g. glutathione) would be expected to lead to progressive oxidative damage to lenses with age, and may ultimately contribute to presbyopia and senile nuclear cataract.
authors.affiliation: Centre for Medical and Health Physics, School of Physical Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland, 4001, Australia.
authors.forename: B A
authors.initials: BA
authors.lastname: Moffat
authors.forename: K A
authors.initials: KA
authors.lastname: Landman
authors.forename: R J
authors.initials: RJ
authors.lastname: Truscott
authors.forename: M H
authors.initials: MH
authors.lastname: Sweeney
authors.forename: J M
authors.initials: JM
authors.lastname: Pope
mesh_terms: D000375:Aging / Q000502:physiology*; D001834:Body Water / Q000378:metabolism*; D002386:Cataract / Q000209:etiology; D004058:Diffusion; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D009924:Organ Culture Techniques; D011305:Presbyopia / Q000209:etiology