Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods.
A fast and quantitative method was developed to detect transgenic "Maximizer" maize "event 176" (Novartis) and "Roundup Ready" soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of "Maximizer" maize and "Roundup Ready" soybean in some representative food products were quantified.
Vaïtilingom
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Tepral, Beverage Division Research Center of Danone Group, 68 Route d'Oberhausbergen, 67037 Strasbourg Cedex, France.
Pijnenburg
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H
Gendre
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Brignon
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D004247:DNA / Q000302:isolation & purification; D017931:DNA Primers; D005818:Genetic Engineering; D006801:Humans; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D013025:Glycine max / Q000737:chemistry* / Q000235:genetics; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics
D017931:DNA Primers; D004247:DNA