title: Modular regulation of cGATA-5 gene expression in the developing heart and gut.
abstract: The evolutionarily conserved GATA-5 transcription factor is an early and persistent marker of heart and gut development in diverse vertebrate species. To search for control regions that might regulate the chicken GATA-5 (cGATA-5) gene, we assayed a set of cGATA-5/lacZ constructs in transgenic mice and found evidence for two functionally conserved control regions that regulate different facets of cGATA-5 gene expression. The more distal control region is activated in embryonic endoderm at the head-fold stage, whereas the other control region contains a regulatory module that is activated in a restricted region of endoderm following closure of the gut tube. Remarkably, the latter control region also contains a complex regulatory module that is activated in the cardiac crescent at the head-fold stage and subsequently functions in several mesodermal components of the developing heart, including the outer (epicardial) layer. We discuss these results in terms of possible contributions of epicardial-derived cells to the formation of heart valves, conduction tissue, and compact myocardium. These transgenes thus reveal, and provide a means to further analyze, transcriptional programs for several facets of heart morphogenesis and gut development.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
authors.forename: C
authors.initials: C
authors.lastname: MacNeill
authors.forename: R
authors.initials: R
authors.lastname: French
authors.forename: T
authors.initials: T
authors.lastname: Evans
authors.forename: A
authors.initials: A
authors.lastname: Wessels
authors.forename: J B
authors.initials: JB
authors.lastname: Burch
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D050992:GATA5 Transcription Factor; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D017403:In Situ Hybridization; D007422:Intestines / Q000196:embryology*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011784:Quail / Q000196:embryology; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D050992:GATA5 Transcription Factor; C494756:Gata5 protein, mouse; D014157:Transcription Factors; D004247:DNA