title: Evaluation of the role of mitotic instability in karyotypically normal men with oligozoospermia.
abstract: OBJECTIVE
  To estimate the incidence of sex chromosome aneuploidies in the somatic cells of karyotypically normal infertile men and fertile donors.
  
  
  METHODS
  A prospective, two-phase, controlled study.
  
  
  METHODS
  Reproductive medicine and medical genetics units of a teaching hospital.
  
  
  METHODS
  Ten patients with oligozoospermia and 10 sperm donors with proved fertility, all with a normal karyotype 46 XY.
  
  
  METHODS
  Multicolor fluorescence in situ hybridization (FISH) of peripheral blood lymphocytes using a probe cocktail containing the alpha satellites DXZ1 for the X centromere and DYZ1 for the heterochromatic region of the long arm of the Y and the radiolabeled alpha satellite D18Z1 for chromosome 18.
  
  
  METHODS
  The incidence of sex chromosome aneuploidies in both groups.
  
  
  RESULTS
  A 13-fold increase in sex chromosome aneuploidies was observed in the somatic cells of infertile men with "unexplained" oligozoospermia compared to donors (P=.008).
  
  
  CONCLUSIONS
  These findings provide suggestive evidence for the existence of an inherent mitotic instability in men with unexplained oligozoospermia.
authors.affiliation: Department of Obstetrics and Gynaecology, Liverpool Women's Hospital, United Kingdom.
authors.forename: M R
authors.initials: MR
authors.lastname: Gazvani
authors.forename: E D
authors.initials: ED
authors.lastname: Wilson
authors.forename: D H
authors.initials: DH
authors.lastname: Richmond
authors.forename: P J
authors.initials: PJ
authors.lastname: Howard
authors.forename: C R
authors.initials: CR
authors.lastname: Kingsland
authors.forename: D I
authors.initials: DI
authors.lastname: Lewis-Jones
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002887:Chromosomes, Human, Pair 18; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008938:Mitosis; D009845:Oligospermia / Q000235:genetics*; D011446:Prospective Studies; D012730:Sex Chromosomes; D014019:Tissue Donors