Plasma diamine oxidase activity is greater in copper-adequate than copper-marginal or copper-deficient rats.
The object of this study was to determine whether serum diamine oxidase activity could distinguish among adequate, marginal and deficient copper status in rats. Male weanling Sprague-Dawley rats (n = 21) were randomly assigned to one of three dietary regimens, with copper concentrations of 0.52, 1.73 and 6.7 mg/kg diet. On completion of the study, body weights were significantly different among dietary groups, with copper-marginal rats displaying the highest mean weight and copper-deficient rats the lowest. Copper-deficient rats ate significantly less food than the other two groups. Rats fed the three diets had significantly different liver copper concentrations. Liver and heart superoxide dismutase and cytochrome c oxidase activities, and plasma ceruloplasmin and erythrocyte superoxide dismutase activities were significantly lower in the copper-deficient rats than in the other two groups. Plasma diamine oxidase activity was lower in both copper-deficient (0.18 +/- 0.11 U/L) and marginal (0.21 +/- 0.11 U/L) rats compared with copper-adequate rats (3.35 +/- 0.28 U/L). Of the biochemical indices measured, only liver copper concentration (-20%) and plasma diamine oxidase activity (-94%) differed between rats fed copper-marginal and copper-adequate diets. Plasma diamine oxidase activity, therefore, may be a sensitive functional biomarker of suboptimal copper status.
Kehoe
C A
CA
Northern Ireland Centre for Diet and Health, University of Ulster at Coleraine, BT52 ISA, Northern Ireland.
Faughnan
M S
MS
Gilmore
W S
WS
Coulter
J S
JS
Howard
A N
AN
Strain
J J
JJ
D006631:Amine Oxidase (Copper-Containing) / Q000097:blood* / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002570:Ceruloplasmin / Q000378:metabolism*; D003300:Copper / Q000008:administration & dosage / Q000172:deficiency / Q000494:pharmacology*; D004032:Diet; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley
D003300:Copper; D002570:Ceruloplasmin; D006631:Amine Oxidase (Copper-Containing)