Wide-field optically sectioning fluorescence microscopy with laser illumination.
We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one-dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.
Neil
M A
MA
Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, U.K.
Squire
A
A
Juskaitis
R
R
Bastiaens
P I
PI
Wilson
T
T
D000818:Animals; D019556:COS Cells / Q000166:cytology / Q000378:metabolism; D007089:Image Enhancement; D007527:Isoenzymes / Q000378:metabolism; D007834:Lasers; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D011493:Protein Kinase C / Q000378:metabolism; D051571:Protein Kinase C-alpha
D007527:Isoenzymes; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha