Characterization of antibodies to prostate-specific antigen.
Antibodies submitted to the ISOBM TD-3 PSA Workshop were tested for reactivity with free prostate-specific antigen (PSA) and the PSA alpha1-antichymotrypsin (ACT) complex, inhibition of proteolytic activity, recognition of continuous epitopes, and epitope specificity based on complete cross-inhibition studies. The antibodies could be separated into two categories: those recognizing hidden epitopes specific for free PSA, and those recognizing epitopes in both free PSA and the PSA-ACT complex. A large number of distinct antigenic domains were identified both in free PSA and the PSA-ACT complex.
Nilsson
O
O
CanAg Diagnostics AB, Gothenburg, Sweden. olle.nilsson@canag.se
Andersson
I
I
Peter
A
A
Karlsson
B
B
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D007118:Immunoassay; D017430:Prostate-Specific Antigen / Q000037:antagonists & inhibitors / Q000276:immunology*; D012661:Semen / Q000378:metabolism; D013997:Time Factors; D000514:alpha 1-Antichymotrypsin / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000514:alpha 1-Antichymotrypsin; D017430:Prostate-Specific Antigen