title: Contortrostatin, a homodimeric disintegrin, binds to integrin alphavbeta5.
abstract: Contortrostatin is a homodimeric disintegrin from snake venom. We have shown that contortrostatin binds to integrins alphaIIbbeta3, alpha5beta1, and alphavbeta3. We now use several criteria to demonstrate the binding of contortrostatin to alphavbeta5. First, incubation of T24 cells, which express alphavbeta3 and alphavbeta5, with antibody against alphavbeta3 failed to completely inhibit adhesion of cells to vitronectin. However, pretreatment of the cells with contortrostatin or the combination of antibodies against alphavbeta3 and alphavbeta5 completely blocked adhesion to vitronectin. By contrast, either anti-alphavbeta5 alone or contortrostatin blocked adhesion of an alphavbeta3-negative T24 subline. Second, contortrostatin as well as anti-alphavbeta5 inhibits invasion of OVCAR-5, which express only alphavbeta5. Third, contortrostatin binds to purified alphavbeta5 in a saturable manner. Finally, radioligand binding assays yielded a K(d) value of 24 nM for [(125)I]contortrostatin binding to alphavbeta5. This investigation identifies alphavbeta5 as a binding site for contortrostatin. Blockage of alphavbeta5 by contortrostatin inhibits alphavbeta5-mediated adhesion and invasion.
authors.affiliation: Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California, 90033, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhou
authors.forename: M T
authors.initials: MT
authors.lastname: Nakada
authors.forename: P C
authors.initials: PC
authors.lastname: Brooks
authors.forename: S D
authors.initials: SD
authors.lastname: Swenson
authors.forename: M R
authors.initials: MR
authors.lastname: Ritter
authors.forename: S
authors.initials: S
authors.lastname: Argounova
authors.forename: C
authors.initials: C
authors.lastname: Arnold
authors.forename: F S
authors.initials: FS
authors.lastname: Markland
mesh_terms: D017836:Agkistrodon; D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects; D019281:Dimerization; D019483:Disintegrins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D009361:Neoplasm Invasiveness; D011869:Radioligand Assay; D019030:Receptors, Vitronectin / Q000737:chemistry / Q000378:metabolism; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms; D014757:Viper Venoms
chemical_list: D019483:Disintegrins; D016023:Integrins; D019030:Receptors, Vitronectin; D014757:Viper Venoms; C403494:contortrostatin; C064660:integrin alphaVbeta5