Analysis of pigment accumulation heterogeneity in plant cell population by image-processing system.
Plant-cultured cells are often highly heterogeneous in secondary metabolite productivity. The industrial application for large-scale metabolite production requires establishment of a stable high-producing cell line. In this study, image analysis of the individual cell is investigated as a method for evaluation of a heterogeneous cell population, and compared with the conventional method of estimation, which is based on average-cell productivity. Among strawberry cells producing anthocyanins, cells with a wide-range of pigment concentration were observed and maximum anthocyanin content was 10 times higher than the average value. In addition, a change of the frequency distribution was revealed in batch cultivation.
Miyanaga
K
K
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. mseki@chembio.t.u-tokyo.ac.jp
Seki
M
M
Furusaki
S
S
D000872:Anthocyanins / Q000032:analysis* / Q000378:metabolism; D002455:Cell Division; D007091:Image Processing, Computer-Assisted; D008853:Microscopy; D010860:Pigments, Biological / Q000032:analysis* / Q000378:metabolism; D010945:Plants, Edible / Q000166:cytology* / Q000378:metabolism*
D000872:Anthocyanins; D010860:Pigments, Biological