Gradients in cytoplasmic calcium concentration ([Ca2+]i) in migrating human umbilical vein endothelial cells (HUVECs) stimulated by shear-stress.
Using a parallel-plate flow-chamber and confocal laser scanning microscopy (CLSM), we studied the distribution and temporal changes in intracellular Ca2+ concentration ([Ca2+]i) in migrating HUVECs stimulated by shear-stress. In the presence or absence of ATP, shear-stress (10 dyne/cm2) caused morphological change and migration of individual HUVECs in the random direction. After 120 minute exposure to shear-stress, 70% of the cells migrated in the direction of flow, whereas, as many as 30% of the cells migrated to the upstream against flow. A nonspecific plasma membrane Ca2+ channel blocker, Ni2+, abolished such responses markedly, suggesting that Ca2+ influx may be essential for shear-stress dependent morphological change and migration of HUVECs. Analysis of [Ca2+]i distribution revealed the appearance of localized [Ca2+]i elevation inside lamellipodium formed in the direction of cell migration. The localized rise in [Ca2+]i might be closely related with morphological change to regulate the direction of cell migration induced by shear-stress.
Yoshikawa
N
N
Dept. of Surgery II. Osaka University Medical School, Suita, Japan.
Ariyoshi
H
H
Aono
Y
Y
Sakon
M
M
Kawasaki
T
T
Monden
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000814:Aniline Compounds; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism*; D005456:Fluorescent Dyes; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D018613:Microscopy, Confocal; D009532:Nickel / Q000494:pharmacology; D013314:Stress, Mechanical; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism; D014966:Xanthenes
D000814:Aniline Compounds; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D014966:Xanthenes; C059715:Fluo-3; D009532:Nickel; D000255:Adenosine Triphosphate; D002118:Calcium