Hepatic outer-ring deiodinase in a Mexican endemic lizard (Sceloporus grammicus).
The kinetic characterization of the outer-ring deiodination pathway using rT(3) (rT(3)-ORD) in male, female, and pregnant female livers of an endemic lizard, Sceloporus grammicus, is reported. The ORD pathway does not have the characteristics of deiodinase type II; it is exclusively carried out by deiodinase type I (DI). DI enzymatic activity in lizard liver contains one of the highest activities reported in vertebrates. This activity is sexually dimorphic, with males presenting the highest activity during the reproductive season. The properties of this enzyme correspond to those described in mammals, such as specificity for rT(3), susceptibility to inhibition by 6-n-propyl-2-thiouracil and gold-thioglucose, cofactor requirement, and kinetic pattern. Unlike other vertebrates, the lizard DI exhibits conspicuous stability in the thermal range of 15 to 42 degrees C and in the pH range of 5.0 to 9.0. Male true kinetic constants exhibit a direct correlation with temperature. This is in agreement with short-term adaptation to microenvironmental changes and the feasible expression of enzymatic forms/variants which, together, endow this lizard species with a greater adaptation to natural daily ambient thermal fluctuations.
Fenton
B
B
Centro de Neurobiología, Campus UNAM-UAQ Juriquilla, Querétaro, Qro., 76001, México.
Valverde-R
C
C
D000818:Animals; D006051:Aurothioglucose / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006863:Hydrogen-Ion Concentration; D007453:Iodide Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008116:Lizards / Q000378:metabolism*; D008297:Male; D011247:Pregnancy; D011441:Propylthiouracil / Q000494:pharmacology; D012621:Seasons; D012727:Sex Characteristics; D013696:Temperature; D014285:Triiodothyronine, Reverse / Q000378:metabolism
D004791:Enzyme Inhibitors; D006051:Aurothioglucose; D014285:Triiodothyronine, Reverse; D011441:Propylthiouracil; D007453:Iodide Peroxidase; D004229:Dithiothreitol