title: Engineering of osteochondral tissue with bone marrow mesenchymal progenitor cells in a derivatized hyaluronan-gelatin composite sponge.
abstract: The aim of this study was to investigate the potential of a composite matrix, containing esterified hyaluronic acid and gelatin, to facilitate the osteochondral differentiation of culture-expanded, bone marrow-derived mesenchymal progenitor cells. The cell loading characteristics and the effects of the matrix on cell differentiation were examined in vitro and in vivo. Empty and cell-loaded composites were cultivated for up to 28 days in a chemically defined medium with or without transforming growth factor-beta1 (TGF-beta1). A type II collagen-rich extracellular matrix was produced by cells loaded in the matrix and cultured in the presence of TGF-beta1. Empty and cell-loaded matrices were also implanted subcutaneously in immunodeficient mice. Three types of implant were used: empty (group I), cell-loaded matrices (Group II), and cell-loaded matrices cultured for 14 days in vitro in defined medium with TGF-beta1 (group III). No osteochondral differentiation was found in implanted empty matrices; however, the matrix supported osteochondrogenic cell differentiation in the cell-loaded implants. Preculture in vitro in a chondrogenic medium increased the percentage of osteochondral tissue found in the constructs after 3 weeks. These results indicate the potential use of this composite matrix for delivery of bone marrow-derived mesenchymal progenitor cells for the repair of chondral and osseous defects. The results also indicate that this composite matrix is useful for in vitro tissue engineering.
authors.affiliation: Department of Orthopaedics, Case Western Reserve University, Cleveland, OH 44106-5000, USA.
authors.forename: P
authors.initials: P
authors.lastname: Angele
authors.forename: R
authors.initials: R
authors.lastname: Kujat
authors.forename: M
authors.initials: M
authors.lastname: Nerlich
authors.forename: J
authors.initials: J
authors.lastname: Yoo
authors.forename: V
authors.initials: V
authors.lastname: Goldberg
authors.forename: B
authors.initials: B
authors.lastname: Johnstone
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D016026:Bone Marrow Transplantation; D002356:Cartilage / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D017690:Cell Transplantation / Q000295:instrumentation*; D002478:Cells, Cultured / Q000187:drug effects / Q000637:transplantation; D016895:Culture Media, Serum-Free; D004952:Esters; D005109:Extracellular Matrix; D005780:Gelatin / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry; D020007:Implants, Experimental; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D011817:Rabbits; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D015503:Surgical Sponges; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D016895:Culture Media, Serum-Free; D004952:Esters; D016212:Transforming Growth Factor beta; D005780:Gelatin; D006820:Hyaluronic Acid