Characterization of the bile acid profile in developing male and female hamsters in response to dietary cholesterol challenge.
The Syrian golden hamster is a frequently used model to study cholesterol and bile acid metabolism as well as cholesterol-induced cholelithiasis. However, diet-induced gallstones seem limited to young male hamsters of certain strains that develop depressed cholate/chenodeoxycholate bile acid ratios. To further elucidate gender and age specific aspects of cholesterol and bile acid metabolism, i.e. a possible age-related bile acid/gallstone relationship, plasma and biliary lipids and bile acid composition were analyzed in male and female hamsters under various physiological conditions of age and diet, the latter formulated with and without dietary cholesterol. During normal development (no cholesterol challenge) the percentage of cholic acid decreased while chenodeoxycholate increased, the shift being more pronounced in males. Furthermore, female hamsters had higher total plasma cholesterol than in males, while hepatic and biliary lipids did not differ. When challenged with excessive dietary cholesterol, female hamsters again developed significantly higher total plasma and hepatic cholesterol concentrations. Biliary lipids and cholesterol gallstone incidence revealed a significant gender effect with male hamsters developing a higher lithogenic index and more gallstones (cholesterol and pigment stones) than females. Female hamsters revealed a lower percentage of chenodeoxycholate and a higher percentage of cholate resulting in a more protective, higher cholate/cheno ratio (1.5 +/- 1.0) than in males (1.0 +/- 0.2). In summary, the bile acid pattern in developing and cholesterol-fed hamsters renders females less susceptible to gallstones, in part because they maintain more favorable biliary lipid and bile acid profiles, characterized by lower molar percentages of biliary cholesterol and chenodeoxycholate.
Trautwein
E A
EA
Foster Biomedical Research Laboratory, Brandeis University, Waltham, MA 02254, USA.
Siddiqui
A
A
Hayes
K C
KC
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001835:Body Weight; D002769:Cholelithiasis / Q000209:etiology; D002791:Cholesterol, Dietary / Q000008:administration & dosage* / Q000009:adverse effects; D006224:Cricetinae; D005260:Female; D008297:Male; D008647:Mesocricetus; D008954:Models, Biological; D009929:Organ Size
D001647:Bile Acids and Salts; D002791:Cholesterol, Dietary