Asymmetric growth and development of the Xenopus laevis retina during metamorphosis is controlled by type III deiodinase.
During the metamorphosis of the Xenopus laevis retina, thyroid hormone (TH) preferentially induces ventral ciliary marginal zone (CMZ) cells to both increase their proliferation and give rise to ipsilaterally projecting ganglion cells. Here we show that dorsal CMZ cells express type III deiodinase (D3), an enzyme that inactivates TH. The dorsal CMZ cells can be induced to proliferate if deiodinase activity is inhibited. D3 or dominant-negative thyroid hormone receptor transgenes inhibit both TH-induced proliferation of the ventral CMZ cells and the formation of the ipsilateral projection. Thus, the localized expression of D3 in the dorsal CMZ cells accounts for the asymmetric growth of the frog retina.
Marsh-Armstrong
N
N
Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA. marsh-armstrong@mail1.ciwemb.edu
Huang
H
H
Remo
B F
BF
Liu
T T
TT
Brown
D D
DD
D000818:Animals; D000963:Antimetabolites; D001973:Bromodeoxyuridine; D003287:Contrast Media / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017403:In Situ Hybridization; D007453:Iodide Peroxidase / Q000037:antagonists & inhibitors / Q000502:physiology*; D007480:Iopanoic Acid / Q000494:pharmacology; D008675:Metamorphosis, Biological / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D012160:Retina / Q000201:enzymology* / Q000254:growth & development*; D013788:Thalamus / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D014795:Visual Pathways / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D014982:Xenopus laevis
D000963:Antimetabolites; D003287:Contrast Media; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; C117274:iodothyronine deiodinase type III; D007453:Iodide Peroxidase; D007480:Iopanoic Acid; D001973:Bromodeoxyuridine