A newly designed tensile tester for cells and its application to fibroblasts.
A tensile test system for cells has been designed and applied to fibroblasts from the rabbit patellar tendon. It consists of a thermostatic test chamber, an inverted fluorescence microscope, micromanipulators, a direct drive linear actuator, a cantilever-type load cell, and a video dimension analyzer (VDA). The test chamber and the microscope are mounted on a vibration isolator. A cell floated in Hanks' balanced salt solution of 37 degrees C is gripped with a pair of micropipettes which have very fine tips (outer diameter = 20 approximately 30 microm, inner diameter = 3 approximately 5 microm) and are coated with a cell adhesive, Cell-Tak, at their ends. One of the micropipettes is fixed to the load cell; the other one is attached to the linear actuator which is used to stretch the cell. Load applied to the cell is measured with the load cell, while elongation of the cell is determined with the VDA using the images of the ends of the micropipettes as markers. The measurement accuracy of the load cell was +/-0.05 microN. All the fibroblasts tested were firmly attached to the micropipettes during tensile testing, and showed local non-uniform deformation. The maximum load and elongation to failure of the cells were 0.9+/-0.2 microN and 86+/-24 microm, respectively.
Miyazaki
H
H
Department of Systems and Human Science, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan.
Hasegawa
Y
Y
Hayashi
K
K
D000818:Animals; D001703:Biophysics / Q000295:instrumentation; D005347:Fibroblasts / Q000502:physiology*; D066298:In Vitro Techniques; D011817:Rabbits; D013314:Stress, Mechanical; D013710:Tendons / Q000166:cytology / Q000502:physiology; D013718:Tensile Strength