Fast separation of oligonucleotide and triplet repeat DNA on a microfabricated capillary electrophoresis device and capillary electrophoresis.
A laser-induced fluorescence detection system coupled with a highly sensitive silicon-intensified target (SIT) camera is successfully applied to the imaging of a band for DNA fragment labeling by fluorescence dye in a microchannel, and to the visualizing of the separation process on a microfabricated chip. We demonstrated that an only 6 mm separation channel is sufficient for the separation of triplet repeat DNA fragment and DNA molecular marker within only 12 s. The separation using the microfabricated capillary electrophoresis device is confirmed to be at least 18 times faster than the same separation carried out by conventional capillary electrophoresis with 24.5 cm effective length. The use of a short capillary with 8.5 cm effective length is also efficient for fast separation of DNA; however, the microchip technology is even faster than capillary electrophoresis using a short capillary.
Ueda
M
M
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan. uedam@fc.ph.tokushima-u.ac.jp
Kiba
Y
Y
Abe
H
H
Arai
A
A
Nakanishi
H
H
Baba
Y
Y
D004247:DNA / Q000032:analysis* / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes; D009841:Oligonucleotides / Q000032:analysis*; D017422:Sequence Analysis, DNA / Q000379:methods
D005456:Fluorescent Dyes; D009841:Oligonucleotides; D004247:DNA