Electroactive hemoglobin-surfactant-polymer biomembrane-like films.
Polyions complex 2C12N+ PSS- was prepared by reacting poly(sodium styrenesulfonate) (Na+ PSS-) with didodecyldimethylammonium bromide (2Cl2N+ Br-). Stable thin films made from 2C12N+ PSS- with incorporated redox protein hemoglobin (Hb) on pyrolytic graphite (PG) electrodes were then characterized by electrochemistry and other techniques. Cyclic voltammetry (CV) of Hb-2C12N+ PSS- films showed a pair of well-defined and nearly reversible peaks for HbFe(III)/Fe(II) couple at about -0.17 V vs. saturated calomel electrode (SCE) in pH 5.5 buffers. The electron transfer rate between Hb and PG electrode was greatly facilitated in microenvironment of 2C12N+ PSS- films. Positions of Soret absorption band suggest that Hb keeps its secondary structure similar to its native state in 2C12N+ PSS- films at the medium pH. The results of X-ray diffraction and differential scanning calorimetry (DSC) suggest synthesized lipid 2C12N+ PSS- films have an ordered bilayer structure intercalated between PSS- polyion layers, and the incorporated Hb expands the layer spacing of the films. HbFe(I), a highly reduced form of Hb, might also be produced in these films at about -1.09 V, and could be used to catalytically reduce organohalide pollutants.
Sun
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H
Department of Chemistry, Beijing Normal University, China.
Ma
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H
Hu
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N
D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D004566:Electrodes; D006454:Hemoglobins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013501:Surface-Active Agents / Q000737:chemistry*; D014961:X-Ray Diffraction
D006454:Hemoglobins; D008567:Membranes, Artificial; D011108:Polymers; D013501:Surface-Active Agents