Secretion granules of the rabbit parotid. Selective removal of secretory contaminants from granule membranes.
A membrane subfraction obtained from secretion granules isolated from rabbit parotid has been shown to be contaminated by residual secretory proteins to an estimated level of 25-30% of its total protein. In the present study an additional contaminant has been identified by improved mixing experiments and by comparative peptide mapping of specific polypeptides recovered from gels of membrane and content subfractions. This contaminant coelectrophoresis with (and probably comprises the bulk of) the majority component of the membrane subfraction (mol wt approximately 40,000). The contaminating polypeptides can be removed to a large extent by treating the membranes with low concentrations of saponin in the presence of 0.3 M Na2SO4. Although this treatment disrupts the typical bilayer structure of the granule membrane, it does not appear to cause dissociation of its phospholipids or bona fide membrane proteins.
Castle
J D
JD
Section of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Palade
G E
GE
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002501:Centrifugation, Zonal; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008970:Molecular Weight; D010306:Parotid Gland / Q000648:ultrastructure*; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011817:Rabbits
D011506:Proteins