Capillary affinity gel electrophoresis: new technique for specific recognition of DNA sequence and the mutation detection on DNA.
The present state of studies on capillary affinity gel electrophoresis, which is a new technique for the specific recognition of a target DNA sequence, is reviewed. This article includes the principle, theory, methods, and applications of this technology. The great potential of capillary affinity gel electrophoresis for the sequence-specific recognition of DNA and the detection of mutations in specific genes is illustrated.
Baba
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Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Shomachi, Japan. ymttbaba@ph.tokushima-u.ac.jp
D000225:Adenine / Q000031:analogs & derivatives / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods*; D011905:Genes, ras / Q000235:genetics; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D011145:Polyvinyls / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods
D009838:Oligodeoxyribonucleotides; D011145:Polyvinyls; C012002:poly(9-vinyladenine); D004247:DNA; D000225:Adenine