Development of positive selectable markers for the fungal pathogen Cryptococcus neoformans.
Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningitis in approximately 10% of patients with AIDS. New selectable markers which confer resistance to G418 or phleomycin when transformed into C. neoformans were made. A hygromycin-selectable marker was modified to allow selection with a single copy of the marker.
Hua
J
J
Edward A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, St. Louis, Missouri 63104, USA.
Meyer
J D
JD
Lodge
J K
JK
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000737:chemistry*; D003455:Cryptococcus neoformans / Q000276:immunology* / Q000302:isolation & purification*; D004352:Drug Resistance, Microbial / Q000502:physiology; D005839:Gentamicins / Q000494:pharmacology; D010692:Phleomycins / Q000494:pharmacology; D012703:Serotyping
D000900:Anti-Bacterial Agents; D015415:Biomarkers; D005839:Gentamicins; D010692:Phleomycins; C010680:antibiotic G 418