title: Inverse alpha(1A) and alpha(1D) adrenoceptor mRNA expression during isolation of hepatocytes.
abstract: It is now well documented that changes in gene expression take place during cell isolation and culture. Here, we report the change in the expression of the mRNAs for alpha(1)-adrenoceptor subtypes, during dissociation of guinea pig liver cells with collagenase. Using Reverse Transcription-Polymerase Chain Reaction (RT-PCR) assays, it was observed that during the isolation procedure, the mRNA for the alpha(1A)-adrenoceptor, normally expressed in whole liver, was degraded and the mRNA for alpha(1D) subtype, barely expressed in whole liver, increased in an actinomycin D-sensitive manner. When the isolation procedure was performed in the presence of cycloheximide, the mRNA for the alpha(1A)-adrenoceptor did not diminish and the induction of the alpha(1D)-adrenoceptor mRNA was even more evident. Our data indicate that cell isolation alters alpha(1)-adrenoceptor mRNA expression.
authors.affiliation: Instituto de Fisiología Celular, Universidad Nacional Autónoma de México Apartado Postal 70-248, Mexico, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: González-Espinosa
authors.forename: D
authors.initials: D
authors.lastname: González-Espinosa
authors.forename: M T
authors.initials: MT
authors.lastname: Romero-Avila
authors.forename: J A
authors.initials: JA
authors.lastname: García-Sáinz
mesh_terms: D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005786:Gene Expression Regulation; D006168:Guinea Pigs; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012313:RNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1; D003609:Dactinomycin; D012313:RNA; D003513:Cycloheximide