The effect of epigallocatechin galleate and sarcophytol A on DNA strand breakage induced by tobacco-specific nitrosamines and stimulated human phagocytes.
The tobacco-specific nitrosamines (TSNAs) are metabolites of nicotine and are major carcinogens in cigarette smoke. Chronic inflammation may promote the carcinogenic effect of these nitrosamines through the generation of oxygen radicals as evidenced by an increase in DNA strand breakage in cultured human lung cells treated with stimulated human phagocytes and TSNAs. Sarcophytol A (SaA), a simple monohydroxycembratetraene isolated from marine soft coral, and (-)-epigallocatechin galleate (EGCG), one of the main constituents of green tea, both inhibit tumor promotion. To evaluate their effect on TSNA-induced genetic damage, cultured human lung cells were pretreated with SaA or EGCG and then exposed to the TSNA 4-(N-methyl-N-n-trosamino)-1-(3-pyridyl)-1-butanone (NNK) and stimulated human phagocytes and then assayed for single-strand DNA breaks. Both SaA and EGCG provided significant protection against the induction of genetic damage in these cells and may prove useful in the chemoprevention of tobacco-induced carcinogenesis.
Weitberg
A B
AB
Roger Williams Cancer Center and The Dept. of Medicine, Brown University, School of Medicine and Boston University Medical Center, Providence RI, USA.
Corvese
D
D
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004224:Diterpenes / Q000494:pharmacology*; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000196:embryology; D009602:Nitrosamines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D018384:Oxidative Stress; D010586:Phagocytes / Q000187:drug effects / Q000502:physiology*; D010947:Plants, Toxic; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014026:Nicotiana / Q000737:chemistry*
D016588:Anticarcinogenic Agents; D004224:Diterpenes; D005609:Free Radicals; D009602:Nitrosamines; D017382:Reactive Oxygen Species; D013662:Tea; D013481:Superoxides; C058761:sarcophytol A; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; D002392:Catechin; D004247:DNA; D006861:Hydrogen Peroxide; C045651:epigallocatechin gallate; D013755:Tetradecanoylphorbol Acetate