The influence of isatin on guanylyl cyclase of rat heart membranes.
The influence of indole-2,3 dione (isatin) on particulate guanylyl cyclase (GC) from rat heart membranes was investigated in the presence of adenylylimidodiphosphate (AMP-PNP). The latter activated GC in a concentration-dependent manner and 100 microM isatin abolished this effect. The IC(50) value, 2 microM, for the inhibition of stimulation of GC induced by 50 microM AMP-PNP, was close to the upper physiological level of isatin. These results indicate that isatin may interact with GC independently of its regulation by natriuretic peptides.
Medvedev
A
A
Institute of Biomedical Chemistry, Academy of Medical Sciences, 10 Pogodinskaya Str., 119832, Moscow, Russia. medvedev@ibmh.msk.su
Sandler
M
M
Glover
V
V
D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006162:Guanylate Cyclase / Q000378:metabolism*; D007510:Isatin / Q000494:pharmacology*; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D051381:Rats
D000266:Adenylyl Imidodiphosphate; D007510:Isatin; D006162:Guanylate Cyclase