Cellular response to exocyclic DNA adducts.
The mutagenic potential of three exocyclic DNA adducts was studied in Escherichia coli and simian kidney cells by incorporating them into single-stranded DNA. Differences in the mutagenic potency of the adducts were observed between hosts: 1,N6-ethenodeoxyadenosine and 3,N4-ethenodeoxycytidine were more mutagenic in simian cells, whereas 1,N2-(1,3-propan-1,3-diyl)-2'-deoxyguanosine was more mutagenic in E. coli. To investigate the cellular response to DNA adducts, a double-stranded DNA vector system was developed. Use of this system showed that 1,N6-ethenodeoxyadenosine blocks DNA synthesis strongly, and DNA synthesis past this adduct was highly accurate in E. coli. The blockage of DNA synthesis was overcome in an error-free manner by the recombination repair mechanism (daughter-strand gap repair).
Moriya
M
M
Department of Pharmacological Sciences, State University of New York at Stony Brook 11794-8651, USA.
Pandya
G A
GA
Johnson
F
F
Grollman
A P
AP
D000818:Animals; D019556:COS Cells; D018736:DNA Adducts; D004260:DNA Repair; D003839:Deoxyadenosines / Q000737:chemistry / Q000378:metabolism*; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008040:Genetic Linkage; D008957:Models, Genetic; D016297:Mutagenesis, Site-Directed
C090855:1,N(2)-(1,3-propano)-2'-deoxyguanosine; D018736:DNA Adducts; D003839:Deoxyadenosines; D003841:Deoxycytidine; C032221:1,N(6)-ethenodeoxyadenosine; C032222:3,N(4)-ethenodeoxycytidine; D003849:Deoxyguanosine