Characterization of the gtfB and gtfC promoters from Streptococcus mutans GS-5.
We have characterized the promoter regions of the gtfB and gtfC genes from Streptococcus mutans GS-5. Using a plasmid-based reporter system, we discovered that the gtfC promoter is an order of magnitude stronger than the gtfB promoter. In addition, we found that a variety of growth conditions failed to affect or discriminate between the expression of the two promoters. Only during the various phases of growth could we demonstrate significant changes in expression. We conclude that gtfB and gtfC promoters are coordinately expressed.
Goodman
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Department of Basic Sciences, University of Southern California School of Dentistry, 925 West 34th Street, Los Angeles, California, 90089-0641, USA. sgoodman@hsc.usc.edu
Gao
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D001483:Base Sequence; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005798:Genes, Bacterial; D017930:Genes, Reporter; D005964:Glucosyltransferases / Q000235:genetics*; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D013295:Streptococcus mutans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
D002241:Carbohydrates; D017931:DNA Primers; D004269:DNA, Bacterial; C036655:1,3-alpha-D-glucan synthase; D005964:Glucosyltransferases; C110463:glucosyltransferase C; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System