title: Flow cytometric DNA ploidy analysis of oral cancer comparison with histologic grading.
abstract: It has been reported that DNA content analysis provides prognostic information eliminating the subjective component involved in routine microscopic decision making. In an attempt to establish a relationship between pathological findings and flow cytometric analysis, 36 formalin-fixed, paraffin-embedded tumour tissue samples were prepared according to Hedley's method and analysed by means of an Epics Profile II flow cytometer. DNA aneuploidy was observed in 15 tumours (41%). A statistically significant correlation was identified between DNA index and mitoses, cellular response and degree of differentiation, but not the ploidy status. It was not possible to identify a significant association of sex, age, and site of the tumour to DNA index. We found a strong correlation between histologic malignancy and DNA index; an increase in DNA index as malignancy score increases was noted. It was concluded that DNA index shows a good correlation with the histologic features of oral cancer, being a complement of differentiation and histologic grading analysis. The use of DNA analysis as a complement to pathological studies would help to diminish the subjective component of assessment of head and neck cancers. Ploidy status was not statistically associated with the differentiation of tumours.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Medicine and Dentistry, University of Santiago de Compostela, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Seoane
authors.forename: J A
authors.initials: JA
authors.lastname: Asenjo
authors.forename: A
authors.initials: A
authors.lastname: Bascones
authors.forename: P I
authors.initials: PI
authors.lastname: Varela-Centelles
authors.forename: M A
authors.initials: MA
authors.lastname: Romero
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D011003:Ploidies
chemical_list: D004273:DNA, Neoplasm