Use of sublimation to prepare solid microbial media with water-insoluble substrates.
A method was developed to deposit a visible layer of water-insoluble compounds via sublimation onto the surface of solid media. The compound is sublimed from a heated aluminum dish containing the compound onto the surface of an inverted, ice-cooled, inoculated agar petri dish. The method results in the deposition of a thin, even layer on the agar surface without the use of solvent. After incubation, clearing zones around colonies indicate the presence of compound-degrading microorganisms.
Alley
J F
JF
Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi 39762-5759, USA. jfa2@ra.msstate.edu
Brown
L R
LR
D000873:Anthracenes / Q000378:metabolism; D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism; D001431:Bacteriological Techniques; D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry*; D009281:Naphthalenes / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism; D010616:Phenanthrenes / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D014835:Volatilization
D000873:Anthracenes; D003470:Culture Media; D009281:Naphthalenes; D010578:Petroleum; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C031721:naphthalene; C031181:phenanthrene; C034020:anthracene