title: [Dependence of properties of polyclonal antibodies to (2'-5')-oligoadenylates on the method of hapten binding to an immunogen].
abstract: To elucidate the antibody-(2'-5')oligoadenylate relation to the mode of the hapten-immunogen conjugation, a new (2'-5')oligoadenylic acid trimer derivative containing a 2'-terminal N6-(5-carboxypentyl)adenosine and its 125I-labeled immunogenic conjugate were synthesized. The immunization with this conjugate and with a conjugate based on the 2',3'-O-[1-(2-carboxyethyl)]ethylidene derivative of the (2'-5')triadenylic acid gave antisera with different affinities toward modified (2'-5')oligonucleotides. Epitopes involved in the (2'-5')oligomer-binding to different antisera were found.
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, Belarus.
authors.forename: T I
authors.initials: TI
authors.lastname: Kulak
authors.forename: E I
authors.initials: EI
authors.lastname: Kvasiuk
authors.forename: S L
authors.initials: SL
authors.lastname: Sentiureva
authors.forename: S M
authors.initials: SM
authors.lastname: Shuliakovskaia
authors.forename: V P
authors.initials: VP
authors.lastname: Matiushevskaia
authors.forename: M V
authors.initials: MV
authors.lastname: Makarenko
authors.forename: I A
authors.initials: IA
authors.lastname: Mikhaĭlopulo
mesh_terms: D000227:Adenine Nucleotides / Q000276:immunology*; D000906:Antibodies / Q000276:immunology*; D003429:Cross Reactions; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D009843:Oligoribonucleotides / Q000276:immunology*; D011863:Radioimmunoassay; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000227:Adenine Nucleotides; D000906:Antibodies; D006241:Haptens; D009843:Oligoribonucleotides; D014614:Vaccines, Synthetic; C023505:2',5'-oligoadenylate