Identification of bdm-1, a gene involved in G protein beta-subunit function and alpha-subunit accumulation.
Targeted disruption of Galpha and Gbeta genes has established the requirement of an intact G protein signaling pathway for optimal execution of several important physiological processes, including pathogenesis, in the chestnut blight fungus Cryphonectria parasitica. We now report the identification of a G protein signal transduction component, beta disruption mimic factor-1, BDM-1. Disruption of the corresponding gene, bdm-1, resulted in a phenotype indistinguishable from that previously observed after disruption of the Gbeta subunit gene, cpgb-1. The BDM-1 deduced amino acid sequence contained several significant clusters of identity with mammalian phosducin, including a domain corresponding to a highly conserved 11-amino acid stretch that has been implicated in binding to the Gbetagamma dimer and two regions of defined Gbeta/phosducin contact points. Unlike the negative regulatory function proposed for mammalian phosducin, the genetic data presented in this report suggest that BDM-1 is required for or facilitates Gbeta function. Moreover, disruption of either bdm-1 or cpgb-1 resulted in a significant, posttranscriptional reduction in the accumulation of CPG-1, a key Galpha subunit required for a range of vital physiological processes.
Kasahara
S
S
Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, College Park, MD 20742-4450, USA.
Wang
P
P
Nuss
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DL
D000595:Amino Acid Sequence; D001203:Ascomycota; D003001:Cloning, Molecular; D005136:Eye Proteins / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020555:GTP-Binding Protein Regulators; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019204:GTP-Binding Proteins / Q000235:genetics*; D005816:Genetic Complementation Test; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010750:Phosphoproteins / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment; D015398:Signal Transduction / Q000235:genetics*; D014774:Virulence
C403526:BDM-1 protein, Cryphonectria parasitica; C091426:CPG-1 protein, Cryphonectria parasitica; D005136:Eye Proteins; D005656:Fungal Proteins; D020555:GTP-Binding Protein Regulators; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; C065631:phosducin; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D020962:Heterotrimeric GTP-Binding Proteins