[Effect of urea on proteolysis of histone H2A in calf thymus nuclei].
The 1 hour incubation of calf thymus nuclei at 37 degrees C leads to a proteolysis of the histones H1, H3 and H2B. Urea does not influence the histone degradation while 1.5 and 2.0 M NaCl lead to the proteolysis of the H2A histone. On this background, 2 M urea restrains the degradation of the H2A histone. It is assumed that hydrogen bonds are very important for the activity of the proteinases and its interaction with the H2A histone.
Tiulenev
V I
VI
Taras Shevchenko Kyiv University.
Konoplich
L A
LA
Rudenko
O A
OA
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D004789:Enzyme Activation; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D006868:Hydrolysis; D012965:Sodium Chloride / Q000737:chemistry; D013379:Substrate Specificity; D013950:Thymus Gland / Q000378:metabolism*; D014508:Urea / Q000737:chemistry*
D006657:Histones; D012965:Sodium Chloride; D014508:Urea; D010450:Endopeptidases