title: Rapid turnover of FlhD and FlhC, the flagellar regulon transcriptional activator proteins, during Proteus swarming.
abstract: The enterobacterial flhDC master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. During Proteus mirabilis differentiation into elongated hyperflagellated swarm cells, flhDC transcription is strongly but transiently increased. We show that concentration of the FlhD and FlhC proteins is also tightly controlled at the posttranslational level. This is achieved by protein degradation, which is most severe after differentiation when the half-life of both proteins is ca. 2 min. Degradation is energy dependent and putatively involves the Lon protease.
authors.affiliation: Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom.
authors.forename: L
authors.initials: L
authors.lastname: Claret
authors.forename: C
authors.initials: C
authors.lastname: Hughes
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005407:Flagella / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D011513:Proteus mirabilis / Q000502:physiology*; D018085:Regulon; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C090552:flhC protein, E coli; C090553:flhD protein, E coli