BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice.
The activation of the mitogen-activated protein (MAP) kinases by different environmental stresses has been previously observed in several dicot plant species. Here, we report the isolation of a novel MAP kinase in rice that is induced during infection by the blast fungus Magnaporthe grisea or upon mechanical wounding. The gene is designated as BWMK1 for blast- and wound-induced MAP kinase. The cDNA of BWMK1 was isolated from rice leaves challenged by the blast pathogen. Transcripts of the corresponding gene accumulated in rice leaves 4 h after blast inoculation and 30 min after mechanical wounding. This gene encodes a 506 amino acid protein that contains a new dual-phosphorylation activation motif TDY and about 150 unique amino acids on its C terminus. In-gel kinase activity and immunoprecipitation assays confirmed that BWMK1 is a functional MAP kinase. These results show that BWMK1 is a new member of the plant MAP kinase family and may mediate both defense and wound signaling in rice.
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Institute of Molecular Agrobiology, National University of Singapore, Singapore.
Fong
S H
SH
Yang
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Wang
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GL
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018745:Genome, Plant; D020082:Magnaporthe / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000276:immunology; D010940:Plant Proteins; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D010940:Plant Proteins; D019000:Phosphotyrosine; C403286:MPK12 protein, Oryza sativa; D020928:Mitogen-Activated Protein Kinases