title: Secondary ischaemia in experimental free flaps--treatment by long acting prostacyclin analogues.
abstract: Secondary postoperative ischaemia due to venous occlusion is the most detrimental insult to free microvascular flaps. In an experimental rat free flap model the efficacy of long acting prostacyclin analogues iloprost (Ilomedin) and cicaprost in venous occlusion induced postoperative ischaemia was studied. Free, microvascular groin flaps were transplanted to the neck and the draining veins were temporarily occluded on the first postoperative day for a total of 20 min. In the untreated control group, haemorrhagic flap necrosis occurred. Intravital microscopy after secondary ischaemia revealed flap areas without reperfusion. The functional vessel density was significantly reduced. Reperfused capillaries were tortuous and significantly dilated. After reperfusion the interstitial leakage of macromolecular dextran increased, indicating loss of microvascular endothelial integrity. Intraarterial and intravenous applications of iloprost were able to diminish the ischaemic effects, giving a flap survival rate of 83%. Similar results were obtained by intravenous and enteral administration of cicaprost. Transcutaneous oxygen partial pressure measurements confirmed the viability of the surviving flaps. We conclude that both iloprost and cicaprost are effective in preventing venous occlusion induced failure of free microvascular groin flaps.
authors.affiliation: Department of Surgery, Klinikum Grosshadern, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Frick
authors.forename: R G
authors.initials: RG
authors.lastname: Baumeister
authors.forename: M D
authors.initials: MD
authors.lastname: Menger
authors.forename: B
authors.initials: B
authors.lastname: Vollmar
authors.forename: U
authors.initials: U
authors.lastname: Wohllaib
authors.forename: B
authors.initials: B
authors.lastname: Wiebecke
mesh_terms: D000818:Animals; D011464:Epoprostenol / Q000031:analogs & derivatives / Q000627:therapeutic use; D006084:Graft Rejection / Q000517:prevention & control; D016285:Iloprost / Q000627:therapeutic use; D007511:Ischemia / Q000188:drug therapy*; D008297:Male; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D011183:Postoperative Complications / Q000188:drug therapy*; D011465:Prostaglandins, Synthetic / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013524:Surgical Flaps / Q000098:blood supply*; D014665:Vasodilator Agents / Q000627:therapeutic use*
chemical_list: D011465:Prostaglandins, Synthetic; D014665:Vasodilator Agents; D011464:Epoprostenol; D016285:Iloprost; C043867:cicaprost; D010100:Oxygen