title: Simultaneous genotyping of human platelet antigens (HPA) 1 through 6 using new sequence-specific primers for HPA-5.
abstract: BACKGROUND
  Polymerase chain reaction using sequence-specific primers is widely used for genotyping human platelet antigens (HPA). However, the results of HPA-5 genotyping are still problematic.
  
  
  METHODS
  New sequence-specific primers were designed for HPA-5 that, together with already published primers, allow simultaneous genotyping of HPA-1, -2, -3, -4, -5, and -6. The reliability of the described protocol was determined by using reference DNA samples as well as samples from healthy blood donors.
  
  
  RESULTS
  All primers produced specific amplification products. The genotype and the previously ascertained phenotype of the tested specimens were in concordance in all cases.
  
  
  CONCLUSIONS
  The described polymerase chain reaction protocol allows rapid, reliable, simultaneous genotyping of HPA-1, -2, -3, -4, -5, and -6.
authors.affiliation: Blood Bank, Charité, Campus Virchow-Klinikum, Medical Faculty of Humboldt University of Berlin, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Meyer
authors.forename: M
authors.initials: M
authors.lastname: Hildebrandt
authors.forename: B
authors.initials: B
authors.lastname: Schulz
authors.forename: R
authors.initials: R
authors.lastname: Blasczyk
authors.forename: A
authors.initials: A
authors.lastname: Salama
mesh_terms: D000483:Alleles; D016824:Antigens, Human Platelet / Q000235:genetics*; D001782:Blood Donors; D017931:DNA Primers / Q000097:blood*; D005838:Genotype; D006801:Humans; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D016824:Antigens, Human Platelet; D017931:DNA Primers