title: Pharmacokinetic study of paeoniflorin in mice after oral administration of Paeoniae radix extract.
abstract: Quantification of paeoniflorin, the principal bioactive component of Paeoniae radix, in mice plasma following oral administration of Paeoniae radix extract was achieved by using a simple and rapid high-performance liquid chromatographic method. The calibration curve for paeoniflorin was linear (r2 = 0.998) over the concentration range 10-200 ng/ml. The coefficients of variation of intra- and inter-day assays were 15.04, 7.31, 6.14, 6.55, 6.63% and 12.71, 6.07, 3.61, 5.51, 4.52% at concentrations of 10, 60, 100, 160, 200 ng/ml, respectively. The recoveries of paeoniflorin from mice plasma were found to be 74.49, 76.83, 80.38 and 80.56% for concentrations of 30, 80, 120 and 160 ng/ml, respectively. The plasma concentration-time curves were fitted with mean terminal half-lives (t(1/2)) of 94.16 min.
authors.affiliation: Graduate Institute of Pharmacognosy Science, Taipei Medical College, Taiwan, Republic of China. 0943@ms12.url.com.tw
authors.forename: L C
authors.initials: LC
authors.lastname: Chen
authors.forename: M H
authors.initials: MH
authors.lastname: Lee
authors.forename: M H
authors.initials: MH
authors.lastname: Chou
authors.forename: M F
authors.initials: MF
authors.lastname: Lin
authors.forename: L L
authors.initials: LL
authors.lastname: Yang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics*; D001565:Benzoates; D001952:Bridged-Ring Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D007700:Kinetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D039821:Monoterpenes; D010936:Plant Extracts / Q000008:administration & dosage*; D010946:Plants, Medicinal; D011786:Quality Control; D015203:Reproducibility of Results; D006027:Glycosides
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D010936:Plant Extracts; C015423:peoniflorin; D006027:Glycosides