Gene transfer into hypothalamic organotypic cultures using an adeno-associated virus vector.
Organotypic cultures of rat hypothalamic slice cultures were successfully transduced using adeno-associated viral vectors. Using nuclear-targeted Lac-Z as the reporter gene, transduction was found to be very effective, occurring in as high as 89% of a specific cell type, the oxytocin neurons, present in the cultured explants. These transduction levels were not accompanied by any deleterious effects in the cultured cells 7 days after transduction. Such an in vitro approach should be valuable for the study of cell-specific gene expression in neurons in the central nervous system for which there are no homologous (surrogate) cell lines.
Keir
S D
SD
Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
House
S B
SB
Li
J
J
Xiao
X
X
Gainer
H
H
D000818:Animals; D000831:Animals, Newborn; D003587:Cytomegalovirus / Q000235:genetics; D000229:Dependovirus; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D007031:Hypothalamus; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009924:Organ Culture Techniques; D010121:Oxytocin / Q000032:analysis; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D010121:Oxytocin; D001616:beta-Galactosidase