title: Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments.
abstract: We have established a transgenic zebrafish line carrying a shuttle vector plasmid (pML4) for detecting mutagens in aquatic environments. The plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the chromosomal DNA. To evaluate the system, we treated embryos of the transgenic fish with N-ethyl-N-nitrosourea (ENU), which induces a dose-dependent increase in the mutation frequency of the target gene. The mutation spectrum was consistent with the proposed mechanism of ENU mutagenesis. Similarly, treating the embryos with benzo[a]pyrene or 2-amino-3, 8-dimethylimidazo[4,5- f]quinoxaline, which are found in naturally polluted water, significantly increased the frequency of mutations in the target gene.
authors.affiliation: Environmental Health Science Division, National Institute for Environmental Studies, Onogawa, Tsukuba 305-0053, Japan. amanuma@nies.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Amanuma
authors.forename: H
authors.initials: H
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Amanuma
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001564:Benzo(a)pyrene / Q000633:toxicity; D004252:DNA Mutational Analysis; D004305:Dose-Response Relationship, Drug; D029968:Escherichia coli Proteins; D005038:Ethylnitrosourea / Q000633:toxicity; D005260:Female; D018628:Gene Dosage; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D016296:Mutagenesis / Q000187:drug effects / Q000235:genetics; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000633:toxicity*; D009154:Mutation; D011810:Quinoxalines / Q000633:toxicity; D000091243:Ribosomal Protein S9; D019076:Transgenes / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D009153:Mutagens; D011810:Quinoxalines; D000091243:Ribosomal Protein S9; C000717190:RpsI protein, E coli; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; C036990:2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline; D005038:Ethylnitrosourea