title: [Metabolism of adenosine and thymidine in healthy females of different ages and females with mastopathies].
abstract: The activity of thymidine kinase, thymidine phosphorylase, adenosine deaminase and 5'-nucleotidase of AMP was studied in tissues of 39 healthy females, as well as blood serum and lymphocytes of 60 healthy females, as well as in 50 patients with fibrocavernous mastopathy aged as 23-70. Comparative determination of adenosine metabolism enzymes activity in lymphocytes was carried out simultaneously with studying some immunological indexes in the organism of the same-aged healthy females and ones with mastopathy. It was revealed that age-related changes in the activity of thymidine kinase in blood serum reflected the analogous changes in enzyme activity in tissues of the healthy women. A direct correlation was established between thymidine kinase activity and age both in the healthy females and those with mastopathy. A significant decrease in activity of thymidine phosphorylase was demonstrated in blood serum of the patients with mastopathy in the age 46-60. Determined 4-fold increase in the activity of adenosine deaminase in serum was accompanied by decreased enzyme activity in lymphocytes and decreased Lymphocyte Blast Transformation Index in the same age range. Changes of immunological status are more expressed in T-system of immunity. The revealed metabolic changes in DNA-precursors metabolism in the patients with mastopathy aged as 46-60 might be one of the reasons of increased risk of oncological disease in this age group.
authors.affiliation: Donetsk State Medical University.
authors.forename: B G
authors.initials: BG
authors.lastname: Borzenko
authors.forename: O O
authors.initials: OO
authors.lastname: Verkhova
authors.forename: V O
authors.initials: VO
authors.lastname: Pomazan
authors.forename: A A
authors.initials: AA
authors.lastname: Gorbachev
authors.forename: A O
authors.initials: AO
authors.lastname: Okhten'
authors.forename: E M
authors.initials: EM
authors.lastname: Leonenko
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000328:Adult; D000367:Age Factors; D000368:Aged; D001941:Breast Diseases / Q000201:enzymology / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D008875:Middle Aged; D013936:Thymidine / Q000378:metabolism*; D013937:Thymidine Kinase / Q000378:metabolism; D013939:Thymidine Phosphorylase / Q000378:metabolism
chemical_list: D013939:Thymidine Phosphorylase; D013937:Thymidine Kinase; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D000241:Adenosine; D013936:Thymidine