title: Morphological changes related to reconstituted acetylcholine release in a release-deficient cell line.
abstract: The membrane changes accompanying Ca(2+)-dependent acetylcholine release were investigated by comparing release-competent and release-incompetent clones of mouse neuroblastoma N18TG-2 cells. No release could be elicited in native N18 cells or in a N18-choline acetyltransferase clone in which acetylcholine synthesis was induced by transfection with the gene for rat choline acetyltransferase. However, acetylcholine release was operative in a To/9 clone which was co-transfected with complementary DNAs from rat choline acetyltransferase and Torpedo mediatophore 16,000 mol. wt subunit. In thin sections, the aspect of resting N18 and To/9 cells was identical: a very dense cytoplasm with practically no vesicle-like organelles. Cells were chemically fixed at different times during a stimulation using A-23187 and Ca2+, and examined following both freeze-fracture and thin section. Stimulation of To/9 cells induced a marked change affecting the intramembrane particles. The number of medium-sized particles (9.9-12.38 nm) increased, while that of the small particles decreased. This change was not observed in control, release-incompetent cell lines. In the To/9 clone (but not in control clones), this was followed by occurrence of a large new population of pits which initially had a large diameter, but subsequently became smaller as their number decreased. Coated depressions and invaginations became abundant after stimulation, suggesting an endocytosis process. By considering the succession of events and by comparison with data from experiments performed on synapses in situ, it is proposed that a particle alteration was the counterpart of acetylcholine release in co-transfected To/9 cells; this was followed by a massive endocytosis.
authors.affiliation: Département de Pharmacologie/APSIC, Centre Médical Universitaire, Genève 4, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Bugnard
authors.forename: P
authors.initials: P
authors.lastname: Sors
authors.forename: E
authors.initials: E
authors.lastname: Roulet
authors.forename: A
authors.initials: A
authors.lastname: Bloc
authors.forename: F
authors.initials: F
authors.lastname: Loctin
authors.forename: Y
authors.initials: Y
authors.lastname: Dunant
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D048429:Cell Size / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary; D004705:Endocytosis / Q000502:physiology; D005614:Freeze Fracturing; D051379:Mice; D008854:Microscopy, Electron; D009447:Neuroblastoma; D051381:Rats; D009435:Synaptic Transmission / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism / Q000648:ultrastructure; D014101:Torpedo; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D018076:DNA, Complementary; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine