N-acetylglucosaminyltransferase V activity in metastatic models of human hepatocellular carcinoma in nude mice.
N-linked beta 1-6 branched oligosaccharides may contribute directly to the malignant phenotype including metastatic potential of tumour cells. Increased beta 1-6 branching was associated with an increased level of N-acetylglucosaminyltransferase V (GnT V). In this report, the tissues from two metastatic models of human hepatocellular carcinoma (HCC) in nude mice were obtained. GnT V activity and mRNA level were determined. Results showed that GnT V activity in highly metastatic LCI-D20 models (Liver Cancer Institute, passage time: 20 days) (413.1+/-86.4U) was much higher than that in low metastatic LCI-D35 model (passage time 35 days) (155.3+/-31.9U). Northern blot showed that the mRNA level of GnT V in two models had no change. During the selection of a highly metastatic LCI-D20 model, GnT V activity increased from 301.6+/-57.3U to 413.1+/-86.4U while the highly metastatic LCI-D20 model acquired higher metastatic ability after selection. When highly metastatic LCI-D20 model tissues were implanted subcutaneously (s.c.), the GnT V activity decreased dramatically from 413.1+/-86.4U to 94.9U. This is the first report that GnT V activity increased in HCC during metastasis in vivo.
Shao
D M
DM
Gene Research Center and Dept. of Biochemistry, Shanghai Medical University and Key Laboratory of Glycoconjugate, Ministry of Public Health, P.R. China.
Wang
Q H
QH
Chen
C
C
Shen
Z H
ZH
Yao
M
M
Zhou
X D
XD
Tang
Z Y
ZY
Gu
J X
JX
D000818:Animals; D015152:Blotting, Northern; D002240:Carbohydrate Sequence; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000473:pathology*; D004790:Enzyme Induction; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000473:pathology*; D008175:Lung Neoplasms / Q000201:enzymology / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000096:biosynthesis; D012641:Selection, Genetic; D014183:Transplantation, Heterologous
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase