title: Prediction of human cDNA from its homologous mouse full-length cDNA and human shotgun database.
abstract: We propose a prediction method for human full-length cDNA by comparing sequence data between human genome shotgun sequence and mouse full-length cDNA. The human genome which is homologous to the mouse full-length cDNA is selected by a homology search program, and the predicted exons are connected at the exon-intron junction which gives the best homology score to the mouse full-length cDNA. The accuracy of the predicted human full-length coding region is 83.3%, and the false positive rate is 16.7%. Five human full-length proteins out of 20 proteins are correctly predicted.
authors.affiliation: Genome Science Laboratory, Tsukuba Lifescience Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki, Japan. fukunisi@rtc.riken.go.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Fukunishi
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Yoshino
authors.forename: H
authors.initials: H
authors.lastname: Konno
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashizaki
mesh_terms: D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D016208:Databases, Factual; D005091:Exons; D006801:Humans; D051379:Mice; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018076:DNA, Complementary