Heterogeneity of the internal structure of PDR1, a family of Ty1/copia-like retrotransposons in pea.
We characterised the extent of heterogeneity among PDR1 elements, a Ty1/copia-like retrotransposon family in pea, by restriction mapping and PCR with primers designed to amplify four functional domains. The data suggest that two main subfamilies of PDR1 differ in the size of their 5'-region. There are also sequence variants and rearranged copies which include a wide range of deletions of different sizes and deletions combined with insertions of host DNA, or inversions of various regions of the retrotransposon. A deletion hotspot has been found at nucleotide position 394, where buffer sequences of 26 bp and 38 bp containing microsatellite motifs have been generated. There is more heterogeneity in the gag domain of PDR1 than in other functional domains, and the extent and pattern of this diversity was assessed among 56 Pisum accessions. We found a higher rate of rearrangement and sequence variation within the gag domain of PDR1 in P. fulvum and P. abyssinicum accessions than would be expected from the degree of insertion site polymorphism. A neighbour-joining phylogenetic tree constructed for gag sequences has a similar branching pattern to the equivalent insertion site tree, implying that the PDR1 family and its gag domain have coevolved with the pea genome. Combining both trees revealed clear and distinct subgroups among the Pisum ssp.
Vershinin
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Department of Applied Genetics, John Innes Centre, Norwich, UK. alexander.vershinin@bbsrc.ac.uk
Ellis
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D001483:Base Sequence; D018744:DNA, Plant; D015321:Gene Rearrangement; D015750:Genes, gag; D018740:Genetic Heterogeneity; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D018626:Retroelements; D012689:Sequence Homology, Nucleic Acid
D018744:DNA, Plant; D018626:Retroelements