title: Phenochalasins, inhibitors of lipid droplet formation in mouse macrophages, produced by Phomopsis sp. FT-0211.
abstract: Phomopsis sp. FT-0211, a soil isolate, was found to produce inhibitors of lipid droplet formation in mouse peritoneal macrophages. Structurally related new compounds designated phenochalasins A and B were isolated from the fermentation broth of the producing strain by solvent extraction, ODS column chromatography and preparative HPLC. Phenochalasin A caused a dose-dependent reduction in the number and size of lipid droplets in macrophages without any cytotoxic effect at least up to 20 microm. On the other hand, phenochalasin B showed inhibition of lipid droplet formation with a severe cytotoxic effect on macrophages.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kitasato University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tomoda
authors.forename: I
authors.initials: I
authors.lastname: Namatame
authors.forename: S
authors.initials: S
authors.lastname: Si
authors.forename: K
authors.initials: K
authors.lastname: Kawaguchi
authors.forename: R
authors.initials: R
authors.lastname: Masuma
authors.forename: M
authors.initials: M
authors.lastname: Namikoshi
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D007211:Indoles / Q000302:isolation & purification* / Q000494:pharmacology*; D007783:Lactones / Q000302:isolation & purification* / Q000494:pharmacology*; D050356:Lipid Metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D007211:Indoles; D007783:Lactones; C402691:phenochalasin A; C402692:phenochalasin B