title: High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections.
abstract: How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-specific CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-specific CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths.
authors.affiliation: Aaron Diamond AIDS Research Center, The Rockefeller University, New York, New York, USA.
authors.forename: X
authors.initials: X
authors.lastname: Jin
authors.forename: M A
authors.initials: MA
authors.lastname: Demoitie
authors.forename: S M
authors.initials: SM
authors.lastname: Donahoe
authors.forename: G S
authors.initials: GS
authors.lastname: Ogg
authors.forename: S
authors.initials: S
authors.lastname: Bonhoeffer
authors.forename: W M
authors.initials: WM
authors.lastname: Kakimoto
authors.forename: G
authors.initials: G
authors.lastname: Gillespie
authors.forename: P A
authors.initials: PA
authors.lastname: Moss
authors.forename: W
authors.initials: W
authors.lastname: Dyer
authors.forename: M G
authors.initials: MG
authors.lastname: Kurilla
authors.forename: S R
authors.initials: SR
authors.lastname: Riddell
authors.forename: J
authors.initials: J
authors.lastname: Downie
authors.forename: J S
authors.initials: JS
authors.lastname: Sullivan
authors.forename: A J
authors.initials: AJ
authors.lastname: McMichael
authors.forename: C
authors.initials: C
authors.lastname: Workman
authors.forename: D F
authors.initials: DF
authors.lastname: Nixon
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D015234:HLA-A Antigens / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015234:HLA-A Antigens