Estrogen receptor-alpha mRNA variants in murine and human tissues.
A side-by-side comparison of several normal mouse and human tissues was undertaken in order to determine if exon-deleted variant ER-alpha mRNAs are expressed in the mouse. The data showed that the complex pattern of ER-alpha alternative splicing that is detected in multiple human tissues was not apparent in murine tissues. Only low levels of an exon-4 deleted ER-alpha transcript were detected in murine tissues, although multiple relatively abundant exon-deleted ER-alpha transcripts were detected in human tissues. The data support a species-specific difference in the expression of ER-alpha variant mRNAs between mouse and human.
Lu
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Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
Dotzlaw
H
H
Leygue
E
E
Murphy
L J
LJ
Watson
P H
PH
Murphy
L C
LC
D017398:Alternative Splicing; D000818:Animals; D047628:Estrogen Receptor alpha; D005091:Exons; D005260:Female; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity
D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D011960:Receptors, Estrogen