Impaired feedback of gastric functions in carboxypeptidase E-deficient mice.
Carboxypeptidase E deficiency as seen in the fat/fat mice is associated with reduced antral somatostatin content but tripling of the progastrin product. Thus, fat/fat mice are able to maintain normal tissue concentrations of bioactive alpha-amidated gastrin in spite of grossly attenuated progastrin processing. After induction of achlorhydria, however, neither the amount of alpha-amidated gastrin nor the total progastrin product increased in the fat/fat mice. This is contrary to what is seen in wild-type mice. Furthermore, the synthesis of antral somatostatin and fundic chromogranin A is also abnormal. Hence the results suggest a breakdown in the feedback loop that regulates gastric acid secretion.
Friis-Hansen
L
L
Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, DK-2100, Denmark.
Rehfeld
J F
JF
D000126:Achlorhydria / Q000139:chemically induced / Q000503:physiopathology; D000818:Animals; D001483:Base Sequence; D043423:Carboxypeptidase H; D002268:Carboxypeptidases / Q000172:deficiency* / Q000235:genetics; D053379:Chromogranin A; D002864:Chromogranins / Q000096:biosynthesis; D017931:DNA Primers / Q000235:genetics; D005246:Feedback; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009853:Omeprazole / Q000494:pharmacology; D011498:Protein Precursors / Q000378:metabolism; D054328:Proton Pump Inhibitors; D011706:Pyloric Antrum / Q000502:physiology; D013004:Somatostatin / Q000378:metabolism
D053379:Chromogranin A; D002864:Chromogranins; D017931:DNA Primers; D005755:Gastrins; D011498:Protein Precursors; D054328:Proton Pump Inhibitors; C013706:big gastrin; D013004:Somatostatin; D002268:Carboxypeptidases; D043423:Carboxypeptidase H; D009853:Omeprazole