title: Effects on skin blood flow by provocation during local analgesia.
abstract: Although topical analgesia cream has been used for several years, little is known about its effects on the microcirculation. Previous studies have shown a vasoconstrictive effect after short application times and a vasodilatation after longer application. It has also been shown that vasomotion does not occur in the analgesized skin. The present study was undertaken to investigate the alterations in skin blood perfusion following local cooling, local heating and pin-pricking after the establishment of analgesia. In 11 healthy volunteers, skin analgesia was attained by use of a eutectic mixture of lidocaine and prilocaine (EMLA, Astra Pain Control AB, Sweden) applied to the skin three hours prior to provocation. The changes in skin blood perfusion, after applying three different provocation methods, were studied using the laser Doppler technique. Local cooling and heating to temperatures of +10 and +45 degrees C, respectively, were applied for 9 s by use of a copper probe (O12 mm). In the pin-prick provocation method, a combined effect of deflection and penetration of the skin to in total 3 mm was attained. Identical provocation methods were applied to placebo treated and untreated skin areas. After heat provocation, significant differences in the perfusion response between the treatments were seen (P < 0.0001). Skin areas treated with analgesia cream responded with a slow increase in perfusion that persisted beyond the four minute measurement period. Placebo and untreated areas decreased their perfusion over time. After cooling a significant reduction in skin perfusion was seen, irrespective of the treatment. Similarly, after pin-pricking a perfusion increase was seen for all treatments. The findings indicate that topical analgesia influences the myogenic control of the blood flow in those vascular plexa measured by laser Doppler following heat provocation. No differences could be seen in the response to pin-pricking and cooling for the different treatments.
authors.affiliation: Department of Biomedical Engineering, Linköping University, University Hospital, Linköping, SE-581 85, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Arildsson
authors.forename: G E
authors.initials: GE
authors.lastname: Nilsson
authors.forename: T
authors.initials: T
authors.lastname: Strömberg
mesh_terms: D000293:Adolescent; D000328:Adult; D000772:Anesthesia, Local; D000779:Anesthetics, Local / Q000494:pharmacology*; D003080:Cold Temperature; D005260:Female; D005542:Forearm; D006358:Hot Temperature; D006801:Humans; D017078:Laser-Doppler Flowmetry; D008012:Lidocaine / Q000494:pharmacology*; D000077442:Lidocaine, Prilocaine Drug Combination; D008297:Male; D008833:Microcirculation / Q000187:drug effects* / Q000502:physiology; D008954:Models, Biological; D010812:Physical Stimulation; D011318:Prilocaine / Q000494:pharmacology*; D012867:Skin / Q000098:blood supply* / Q000187:drug effects*; D012879:Skin Physiological Phenomena / Q000187:drug effects; D012881:Skin Temperature
chemical_list: D000779:Anesthetics, Local; D000077442:Lidocaine, Prilocaine Drug Combination; D011318:Prilocaine; D008012:Lidocaine