TCF is the nuclear effector of the beta-catenin signal that patterns the sea urchin animal-vegetal axis.
The mechanism of animal-vegetal (AV) axis formation in the sea urchin embryo is incompletely understood. Specification of the axis is thought to involve a combination of cell-cell signals and as yet unidentified maternal determinants. In Xenopus the Wnt pathway plays a crucial role in defining the embryonic axes. Recent experiments in sea urchins have shown that at least two components of the Wnt signaling pathway, GSK3beta and beta-catenin, are involved in embryonic AV axis patterning. These results support the notion that the developmental network that regulates axial patterning in deuterostomes is evolutionarily conserved. To further test this hypothesis, we have examined the role of beta-catenin nuclear binding partners, members of the TCF family of transcriptional regulators, in sea urchin AV axis patterning. To test the role of TCFs in mediating beta-catenin signals in sea urchin AV axis development we examined the consequences of microinjecting RNAs encoding altered forms of TCF on sea urchin development. We show that expression of a dominant negative TCF results in a classic "animalized" embryo. In contrast, microinjected RNA encoding an activated TCF produces a highly "vegetalized" embryo. We show that the transactivational activity of endogenous sea urchin TCF is potentiated by LiCl treatment, which vegetalizes embryos by inhibiting GSK3, consistent with an in vivo interaction between endogenous beta-catenin and TCF. We also provide evidence indicating that all of beta-catenin's activity in patterning the sea urchin AV axis is mediated by TCF. Using a glucocorticoid-responsive TCF, we show that TCF transcriptional activity affects specification along the AV axis between fertilization and the 60-cell stage.
Vonica
A
A
Department of Anatomy, College of Physicians and Surgeons, New York, New York, 10032, USA.
Weng
W
W
Gumbiner
B M
BM
Venuti
J M
JM
D000818:Animals; D000943:Antigens, Differentiation; D019521:Body Patterning; D015820:Cadherins / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002450:Cell Communication; D002454:Cell Differentiation; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005938:Glucocorticoids / Q000494:pharmacology; D038362:Glycogen Synthase Kinase 3; D024002:HMGB Proteins; D018021:Lithium Chloride / Q000494:pharmacology; D008845:Microinjections; D011485:Protein Binding; D012617:Sea Urchins / Q000196:embryology*; D051820:TCF Transcription Factors; D013997:Time Factors; D015534:Trans-Activators; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors / Q000378:metabolism*; D051176:beta Catenin
D000943:Antigens, Differentiation; D015820:Cadherins; D003598:Cytoskeletal Proteins; D005938:Glucocorticoids; D024002:HMGB Proteins; D051820:TCF Transcription Factors; D015534:Trans-Activators; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors; D051176:beta Catenin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3; D018021:Lithium Chloride