Regional pulmonary blood flow during partial liquid ventilation in normal and acute oleic acid-induced lung-injured piglets.
OBJECTIVE
To determine the spatial distribution of pulmonary blood flow in three groups of piglets: partial liquid ventilation in normal piglets, partial liquid ventilation during acute lung injury, and conventional gas ventilation during acute lung injury.


METHODS
Prospective randomized study.


METHODS
A university medical school laboratory approved for animal research.


METHODS
Neonatal piglets.


METHODS
Regional pulmonary blood flow was studied in 21 piglets in the supine position randomized to three different groups: a normal group that received partial liquid ventilation (Normal-PLV) and two acute lung injury groups that received an oleic acid-induced lung injury: partial liquid ventilation during acute lung injury (OA-PLV) and conventional gas ventilation during acute lung injury (OA-Control). Acute lung injury was induced by infusing oleic acid (0.15 mL/kg iv) over 30 mins. Partial liquid ventilation was instituted with perflubron (LiquiVent, 30 mL/kg) after 30 mins in the Normal-PLV and OA-PLV groups.


RESULTS
Arterial and venous blood gases, hemodynamics, and pulmonary mechanics were measured every 15 mins throughout the hour-long study. Pulmonary blood flow was assessed by fluorescent microsphere technique at baseline and after 30, 45, and 60 mins. In the Normal-PLV piglets, pulmonary blood flow decreased from baseline (before injury or partial liquid ventilation) in the most dependent areas of the lung (F ratio = 3.227; p < .001). In the OA-PLV piglets, pulmonary blood flow was preserved over time throughout the lung (F ratio = 1.079; p = .38). In the OA-Control piglets, pulmonary blood flow decreased in the most dependent areas of the lung and increased from baseline in less dependent slices over time (F ratio = 2.48; p = .003).


CONCLUSIONS
The spatial distribution of regional pulmonary blood flow is preserved during partial liquid ventilation compared with gas ventilation in oleic acid-induced lung injury.
Enrione
M A
MA
Division of Pediatric Critical Care Medicine, University of California at Davis Medical Center, Sacramento, USA.
Papo
M C
MC
Leach
C L
CL
Holm
B A
BA
Hernan
L J
LJ
Fuhrman
B P
BP
Dowhy
M S
MS
Rath
M G
MG
Frisicaro
P E
PE
D000818:Animals; D000831:Animals, Newborn; D006439:Hemodynamics; D008171:Lung Diseases / Q000139:chemically induced / Q000503:physiopathology* / Q000628:therapy; D008863:Microspheres; D019301:Oleic Acid; D011652:Pulmonary Circulation; D011659:Pulmonary Gas Exchange; D012121:Respiration, Artificial / Q000379:methods*; D013552:Swine
D019301:Oleic Acid