title: Preparation and in vivo testing of porous alumina ceramics for cell carrier applications.
abstract: Microporous alumina was used to develop implantable cell carriers shaped as a hollow-sphere with a central opening to allow ingrowth of vascularised tissues. The carriers were produced by suspending the ceramic raw materials in water, homogenising and dropping the resulting slurry onto a heated plate (hot plate moulding, HPM). Morphological characteristics of the cell carriers were investigated by SEM and optical microscopy. Produced carriers had an average diameter of 4.9 mm. The material was highly porous (56 +/- 8%). For in vivo testing the cell carriers were implanted into abdominal wall of Zur: SIV rats for up to 50 weeks and investigated by light microscopy, SEM and TEM. The surface of the hollow carriers was in close contact with unirritated muscle tissue; no inflammation or capsule formation was observed. Loose connective tissue had grown into the hollow cell carrier, and after prolonged implantation >20 weeks adipocytes were observed. The absence of scar tissue formation around the implant and the vitality within the cavity of the hollow carriers indicate that porous alumina may be used for cell transplantation devices.
authors.affiliation: Department of Materials, ETH Zurich, Schlieren, Switzerland. eckert@biocomp.mat.ethz.ch
authors.forename: K L
authors.initials: KL
authors.lastname: Eckert
authors.forename: M
authors.initials: M
authors.lastname: Mathey
authors.forename: J
authors.initials: J
authors.lastname: Mayer
authors.forename: F R
authors.initials: FR
authors.lastname: Homberger
authors.forename: P E
authors.initials: PE
authors.lastname: Thomann
authors.forename: P
authors.initials: P
authors.lastname: Groscurth
authors.forename: E
authors.initials: E
authors.lastname: Wintermantel
mesh_terms: D000537:Aluminum Oxide; D000818:Animals; D001672:Biocompatible Materials; D017690:Cell Transplantation / Q000379:methods*; D002516:Ceramics; D003239:Connective Tissue Cells / Q000637:transplantation / Q000648:ultrastructure; D005260:Female; D005347:Fibroblasts / Q000637:transplantation / Q000648:ultrastructure; D020007:Implants, Experimental; D007963:Leukocytes, Mononuclear / Q000637:transplantation / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D000537:Aluminum Oxide