Reduced mobilization of CD34+ stem cells in advanced human immunodeficiency virus type 1 disease.
Granulocyte colony-stimulating factor (r-met Hu G-CSF; filgrastim; 10 microgram/kg/day for 7 days) was used to mobilize CD34+stem cells into the peripheral blood of human immunodeficiency virus type 1 (HIV-1)-infected individuals and a group of HIV-1-uninfected donors as a measure of immunologic reserve in HIV-1-infected people. G-CSF mobilized CD34+ cells of HIV-1-infected individuals with cell counts >500 CD4+ cells/mm3, as well as in HIV-1-uninfected donors. In contrast, CD34 cell mobilization was significantly blunted in HIV-1-infected individuals with cell counts <500 CD4+ cells/mm3 (<200 cell days vs. >650 cell days, P<.0005, compared with the >500 CD4+ cell cohort). At least 1.75x10(7) CD34 cells were harvested by leukapheresis from patients in each study cohort. CD34+ cell viability and the ability to differentiate precursor cells into myeloid and erythroid progenitor cells were not affected by HIV-1 infection.
Schooley
R T
RT
University of Colorado Health Sciences Center, Division of Infectious Diseases, Denver, CO 80262, USA. Robert.Schooley@uchsc.edu
Mladenovic
J
J
Sevin
A
A
Chiu
S
S
Miles
S A
SA
Pomerantz
R J
RJ
Campbell
T B
TB
Bell
D
D
Ambruso
D
D
Wong
R
R
Landay
A
A
Coombs
R W
RW
Fox
L
L
Kamoun
M
M
Jacovini
J
J
D000328:Adult; D018952:Antigens, CD34; D018791:CD4 Lymphocyte Count; D015331:Cohort Studies; D004279:DNA, Viral / Q000097:blood; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000097:blood; D011994:Recombinant Proteins; D016176:T-Lymphocyte Subsets
D018952:Antigens, CD34; D004279:DNA, Viral; D012367:RNA, Viral; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim