Aptamers containing thymidine 3'-O-phosphorodithioates: synthesis and binding to nuclear factor-kappaB.
Aptamers targeting NF-kappaB containing thymidine 3'-O-phosphorodithioates in selected positions of an oligonucleotide duplex were synthesized. Binding affinities to NF-kappaB varied with the number and positions of the dithioate backbone substitutions. One of the aptamers showed specific binding to a single NF-kappaB dimer in cell culture extracts.
Yang
X
X
Sealy Center for Structural Biology, Department of Human Biological Chemistry & Genetics, The University of Texas Medical Branch at Galveston, 77555-1157, USA.
Fennewald
S
S
Luxon
B A
BA
Aronson
J
J
Herzog
N K
NK
Gorenstein
D G
DG
D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D016328:NF-kappa B / Q000378:metabolism*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000032:analysis*
D016328:NF-kappa B; D009841:Oligonucleotides; D009946:Organothiophosphorus Compounds