title: Aptamers containing thymidine 3'-O-phosphorodithioates: synthesis and binding to nuclear factor-kappaB.
abstract: Aptamers targeting NF-kappaB containing thymidine 3'-O-phosphorodithioates in selected positions of an oligonucleotide duplex were synthesized. Binding affinities to NF-kappaB varied with the number and positions of the dithioate backbone substitutions. One of the aptamers showed specific binding to a single NF-kappaB dimer in cell culture extracts.
authors.affiliation: Sealy Center for Structural Biology, Department of Human Biological Chemistry & Genetics, The University of Texas Medical Branch at Galveston, 77555-1157, USA.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: S
authors.initials: S
authors.lastname: Fennewald
authors.forename: B A
authors.initials: BA
authors.lastname: Luxon
authors.forename: J
authors.initials: J
authors.lastname: Aronson
authors.forename: N K
authors.initials: NK
authors.lastname: Herzog
authors.forename: D G
authors.initials: DG
authors.lastname: Gorenstein
mesh_terms: D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D016328:NF-kappa B / Q000378:metabolism*; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D009946:Organothiophosphorus Compounds / Q000032:analysis*
chemical_list: D016328:NF-kappa B; D009841:Oligonucleotides; D009946:Organothiophosphorus Compounds