High frequency of cytomegalovirus-specific cytotoxic T-effector cells in HLA-A*0201-positive subjects during multiple viral coinfections.
How the cellular immune response copes with diverse antigenic competition is poorly understood. Responses of virus-specific cytotoxic T lymphocytes (CTL) were examined longitudinally in an individual coinfected with human immunodeficiency virus type 1 (HIV-1), Epstein-Barr virus (EBV), and cytomegalovirus (CMV). CTL responses to all 3 viruses were quantified by limiting dilution analysis and staining with HLA-A*0201 tetrameric complexes folded with HIV-1, EBV, and CMV peptides. A predominance of CMV-pp65-specific CTL was found, with a much lower frequency of CTL to HIV-1 Gag and Pol and to EBV-BMLF1 and LMP2. The high frequency of CMV-specific CTL, compared with HIV-1- and EBV-specific CTL, was confirmed in an additional 16 HLA-A*0201-positive virus-coinfected subjects. Therefore, the human immune system can mount CTL responses to multiple viral antigens simultaneously, albeit with different strengths.
Jin
X
X
Aaron Diamond AIDS Research Center, The Rockefeller University, New York, New York, USA.
Demoitie
M A
MA
Donahoe
S M
SM
Ogg
G S
GS
Bonhoeffer
S
S
Kakimoto
W M
WM
Gillespie
G
G
Moss
P A
PA
Dyer
W
W
Kurilla
M G
MG
Riddell
S R
SR
Downie
J
J
Sullivan
J S
JS
McMichael
A J
AJ
Workman
C
C
Nixon
D F
DF
D000328:Adult; D003430:Cross-Sectional Studies; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D015234:HLA-A Antigens / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology*
D015234:HLA-A Antigens