Constitutive expression of GlyCAM-1 core protein in the rat cochlea.
Glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1) is a mucin-like glycoprotein previously identified on high endothelial venules (HEV) of lymph nodes and also in lactating mammary glands. A specifically glycosilated form of GlyCAM-1 on HEV has been shown to be a ligand for a leukocyte L-selectin, which plays an important role in leukocyte rolling along the inflamed endothelium. Here we report that GlyCAM-1 is also expressed in the cochlea. Immunohistochemistry revealed the lateral wall of the cochlea, tectorial membrane, modiolus, organ of corti, and spiral modiolar vein (SMV) to be strongly stained with polyclonal anti-GlyCAM-1 antibody. Moreover, RT-PCR of the cochlear tissue by the use of specific oligonucleotide primers for rat GlyCAM-1 generated a 378 bp product which was then verified by nucleotide sequencing to represent GlyCAM-1. Electron microscopic investigation revealed the presence of GlyCAM-1 over the entire lumenal surface of the vessels, and the basolateral infoldings in stria vascularis. However, soluble L-selectin or mAb MECA-79 which recognizes a carbohydrate epitope on functional L-selectin ligands bound only to the spiral ligament, tectorial membrane and modiolus. These observations suggest that GlyCAM-1 expressed in the cochlear region is heterogenous in terms of its glycosylation.
Kanoh
N
N
Department of Otolaryngology, Hyogo College of Medicine, Nishinomiya, Japan. nkanoh@hyo-med.ac.jp
Dai
C F
CF
Tanaka
T
T
Izawa
D
D
Li
Y F
YF
Kawashima
H
H
Miyasaka
M
M
D000818:Animals; D001483:Base Sequence; D003051:Cochlea / Q000378:metabolism* / Q000648:ultrastructure; D006031:Glycosylation; D007074:Immunoglobulin G / Q000378:metabolism; D007150:Immunohistochemistry; D019041:L-Selectin / Q000378:metabolism; D008024:Ligands; D008297:Male; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009077:Mucins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D009925:Organ of Corti / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013316:Stria Vascularis / Q000378:metabolism
D007074:Immunoglobulin G; D008024:Ligands; D009077:Mucins; D019041:L-Selectin; C075185:sulfated glycoprotein p50