Human DNA adducts of 1,3-butadiene, an important environmental carcinogen.
The N-1-(2,3,4-trihydroxybutyl)adenine (N-1-THB-Ade) adducts induced by 1,3-butadiene (BD) were analysed from lymphocytes of 15 workers occupationally exposed to BD and 11 controls by (32)P-post-labelling using HPLC with radioactivity detection. The difference in the adduct levels between the BD-exposed workers (4.5 +/- 7.7 adducts/10(9) nucleotides) and the controls (0.8 +/- 1.2 adducts/10(9) nucleotides) was statistically significant (Wilcoxon rank sum test, P = 0.038). This study shows for the first time BD-induced DNA adducts in humans and suggests that N-1-THB-Ade adducts may be used to biomonitor human exposure to BD.
Zhao
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Center for Nutrition and Toxicology, Department of Biosciences, Karolinska Institute, NOVUM, 141 57 Huddinge, Sweden. chunyan.zhao@cnt.ki.se
Vodicka
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Srám1
R J
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Hemminki
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D000328:Adult; D000368:Aged; D002070:Butadienes / Q000378:metabolism*; D002274:Carcinogens, Environmental / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts / Q000032:analysis*; D004784:Environmental Monitoring; D006801:Humans; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000009:adverse effects
D002070:Butadienes; D002274:Carcinogens, Environmental; D018736:DNA Adducts; D004247:DNA; C031763:1,3-butadiene