Reconstitution of protein transport across the vacuolar membrane in Plasmodium falciparum-infected permeabilized erythrocytes.
The parasite Plasmodium falciparum induces morphological and biochemical alterations of its host erythrocyte. Some of these changes are mediated by parasite proteins that are transported to specific destinations within the erythrocyte or to the erythrocyte plasma membrane. The pathways underlying this transport are still unknown. We anticipate that at least some aspects of these pathways may be biologically unique and therefore potential targets for chemotherapeutic intervention. We have utilized bacterial pore-forming proteins to establish an experimental system that allows selective permeabilization of the erythrocyte plasma membrane, without affecting the integrity of the vacuolar membrane and the parasite plasma membrane, in order to study protein transport from the parasite into the host erythrocyte. Physiological properties of the parasite within permeabilized erythrocytes, such as the ability to synthesize proteins, will be described. The permeabilization of infected erythrocytes has allowed the dissection of individual steps in protein transport from the parasite surface across the vacuolar membrane. Possible pathways involved in the trafficking of parasite proteins within the erythrocyte cytosol, i.e. in a cell that normally has no need to transport proteins, will be discussed.
Baumeister
S
S
FB Biologie/Zoologie, Philipps Universität Marburg, Germany.
Burgwedel
A
A
Maier
U G
UG
Lingelbach
K
K
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004912:Erythrocytes / Q000469:parasitology*; D006790:Host-Parasite Interactions; D010963:Plasmodium falciparum; D014617:Vacuoles / Q000378:metabolism / Q000469:parasitology
D001798:Blood Proteins