Flow injection monitoring and analysis of mixtures of hydrazine compounds using filter-supported bilayer lipid membranes with incorporated DNA.
This work describes a technique for the rapid and sensitive electrochemical flow injection monitoring and analysis of mixtures of hydrazine compounds using stabilized systems of filter-supported bilayer lipid membranes (BLMs) composed of egg phosphatidylcholine (egg PC) with incorporated DNA. Injections of hydrazines were made into flowing streams of a carrier electrolyte solution, and a transient current signal with a duration of seconds reproducibly appeared in less than one min after exposure of the DNA-modified lipid membranes to the hydrazines. The magnitude of this signal was linearly related to the concentration of hydrazines, which could be determined at sub-micromolar levels. Repetitive cycles of injection of hydrazines have shown no signal degradation during each cycle (30 sequential injections). The time of appearance of the transient response was different for each hydrazine and increased in the order of hydrazine, methylhydrazine or dimethylhydrazine, and phenylhydrazine. The difference in time of response has allowed selective detection and analysis of these hydrazines in mixtures.
Siontorou
C G
CG
Department of Chemistry, University of Athens, Greece.
Nikolelis
D P
DP
Krull
U J
UJ
D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D005374:Filtration / Q000295:instrumentation; D017022:Flow Injection Analysis / Q000379:methods; D006834:Hydrazines / Q000032:analysis*; D008051:Lipid Bilayers / Q000737:chemistry*
D006834:Hydrazines; D008051:Lipid Bilayers; D004247:DNA