Primary biodegradation of a series of alkyl sulfosuccinates by mixed bacterial culture.
A mixed bacterial culture capable of primary biodegradation of sodium alkyl sulfosuccinates R1-OOC-CH(SO3Na)-CH2-COO-R2 was obtained from soil microorganisms by enrichment cultivation and adaptation in the presence of mono-n-dodecyl sulfosuccinate. Gram-negative psychrophilic bacteria with proteolytic, lipolytic and ammonifying activities were prevalent in the culture. The process of primary biodegradation of alkyl sulfosuccinates can be described by first-order reaction kinetics. The rate constants for linear esters were ascending in the order C4 < C5 < C6 (45 mumol/min per g cell protein) and further descending with increasing length of the carbon chain C6 > C8 >> C13. Substitution of cyclohexyl for n-hexyl group resulted in fourfold decrease in biodegradation rate. Terminal branching of alkyl chain does not affect the rate of primary biodegradation.
Gregorová
D
D
Department of Biochemistry and Microbiology, Faculty of Chemical Technology, Slovak University of Technology, Bratislava, Slovakia.
Augustín
J
J
Vrbanová
A
A
Sládeková
D
D
Cserháti
T
T
D000220:Adaptation, Biological; D000473:Alkanes / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003902:Detergents / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism*; D013463:Sulfuric Acid Esters / Q000378:metabolism
D000473:Alkanes; D003902:Detergents; D013386:Succinates; D013463:Sulfuric Acid Esters; C110836:di-n-hexyl sulfosuccinate