Cloning and characterization of a Drosophila melanogaster cDNA encoding a glutamate transporter.
A Drosophila cDNA encoding a glutamate transporter was cloned and examined. The predicted protein (479 amino acid residues) shows significant sequence identity with mammalian counterparts. The protein expressed in Xenopus oocytes had a glutamate transport activity. Northern blot analysis showed that the transcript increased in amount developmentally. This expression pattern is different from those of Drosophila glutamate receptors.
Kawano
T
T
Department of Biological and Environmental Chemistry, Faculty of Agriculture, Tottori University, Japan. kawano@muses.tottori-u.ac.jp
Takuwa
K
K
Kuniyoshi
H
H
Juni
N
N
Nakajima
T
T
Yamamoto
D
D
Kimura
Y
Y
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D027322:Amino Acid Transport System X-AG; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary; D011994:Recombinant Proteins; D018698:Glutamic Acid