title: Unusual case of Acanthamoeba polyphaga and Pseudomonas aeruginosa keratitis in a contact lens wearer from Gauteng, South Africa.
abstract: Acanthamoeba species can cause a chronic, progressive ulcerative keratitis of the eye which is not responsive to the usual antimicrobial therapy and is frequently mistaken for stromal herpes keratitis. An unusual case of coinfection with Acanthamoeba polyphaga and Pseudomonas aeruginosa as causes of corneal keratitis in a contact lens wearer from Gauteng, South Africa, is reported. These two pathogens have previously been assumed to be selectively exclusive. Cysts of the isolated acanthameba tolerated an incubation temperature of 40 degrees C, indicating a pathogenic species. This case highlights the importance of culture methods in the diagnosis of corneal infection and the choice of treatment regimen. The patient's history of careless contact lens-disinfecting habits emphasizes the need to adhere strictly to recommended methods of contact lens care.
authors.affiliation: Department of Clinical Microbiology and Infectious Diseases, South African Institute for Medical Research and University of the Witwatersrand, University of the Witwatersrand, Johannesburg, South Africa. leigh_dini@hotmail.com
authors.forename: L A
authors.initials: LA
authors.lastname: Dini
authors.forename: C
authors.initials: C
authors.lastname: Cockinos
authors.forename: J A
authors.initials: JA
authors.lastname: Frean
authors.forename: I A
authors.initials: IA
authors.lastname: Niszl
authors.forename: M B
authors.initials: MB
authors.lastname: Markus
mesh_terms: D015823:Acanthamoeba Keratitis / Q000150:complications* / Q000175:diagnosis; D000328:Adult; D000818:Animals; D003263:Contact Lenses, Hydrophilic; D003320:Corneal Ulcer / Q000150:complications* / Q000175:diagnosis; D006801:Humans; D011552:Pseudomonas Infections / Q000150:complications* / Q000175:diagnosis; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification