title: Genetic Engineering of Streptomyces coelicolor A3(2) for the Enantioselective Reduction of Unnatural beta-Keto-Ester Substrates.
abstract: Potential "reagents" for the enantioselective reduction, and other biotransformations, of beta-keto-esters result from the genetic engineering of Streptomyces coelicolor A3(2). For example, incubation of the N-acetylcysteamine thioester 1 with the recombinant strain CH999/pIJ5675 followed by treatment with MeOH/HCl gave the lactone 2 as essentially a single enantiomer.
authors.affiliation: School of Chemical Sciences University of East Anglia Norwich NR4 7TJ (UK).
authors.initials: CE
authors.lastname: Anson
authors.initials: MJ
authors.lastname: Bibb
authors.initials: KI
authors.lastname: Booker-Milburn
authors.initials: C
authors.lastname: Clissold
authors.initials: PJ
authors.lastname: Haley
authors.initials: DA
authors.lastname: Hopwood
authors.initials: K
authors.lastname: Ichinose
authors.initials: WP
authors.lastname: Revill
authors.initials: GR
authors.lastname: Stephenson
authors.initials: CM
authors.lastname: Surti