Recent advances in the molecular genetics of Paramecium.
Paramecium continues to be used to study motility, behavior, exocytosis, and the relationship between the germ and the somatic nuclei. Recent progress in molecular genetics is described. Toward cloning genes that correspond to mutant phenotypes, a method combining complementation with microinjected DNA and library sorting has been used successfully in cloning several novel genes crucial in membrane excitation and in trichocyst discharge. Paramecium transformation en masse has now been shown by using electroporation or bioballistics. Gene silencing has also been discovered in Paramecium, recently. Some 200 Paramecium genes, full length or partial, have already been cloned largely by homology. Generalizing the use of gene silencing and related reverse-genetic techniques would allow us to correlate these genes with their function in vivo.
Kung
C
C
Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA. ckung@facstaff.wisc.edu
Saimi
Y
Y
Haynes
W J
WJ
Ling
K Y
KY
Kissmehl
R
R
D000818:Animals; D003001:Cloning, Molecular; D020868:Gene Silencing; D017125:Genes, Protozoan; D008967:Molecular Biology; D010247:Paramecium / Q000235:genetics*; D010641:Phenotype; D014170:Transformation, Genetic