Application of high-density optical microwell arrays in a live-cell biosensing system.
In this paper, we use optical imaging fibers to fabricate a chemical and biochemical sensor that utilizes the ability of living cells to respond to biologically significant compounds. The sensor is created by randomly dispersing single NIH 3T3 mouse fibroblast cells into an optically addressable fiber-optic microwell array such that each microwell accommodates a single cell. The cells are encoded to identify their location within the array and to correlate changes or manipulations in the local environment to responses of specific cell types. The entire array can be simultaneously measured, yielding a rapid, repetitive, and high-density analysis method.
Taylor
L C
LC
Max Tishler Laboratory for Organic Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Walt
D R
DR
D016475:3T3 Cells; D000818:Animals; D001681:Biological Assay / Q000379:methods; D015374:Biosensing Techniques / Q000379:methods*; D051379:Mice