title: Epstein-Barr virus status and the histopathological changes of parotid gland lymphoid infiltrates in HIV-positive children.
abstract: This study examined the EBV status and the morphology in parotid glands of a large cohort of HIV-positive pediatric patients. Nineteen children with vertically acquired HIV infection, ranging in age from three months to seven years and two months, were analyzed. Seventeen patients were assessed for serological evidence of EBV infection; nine showed evidence of past infection, one each re-activation and current infection and six did not have serological evidence of EBV. Immunohistochemistry and in situ hybridization for EBER 1 and 2 were performed on formalin-fixed, paraffin-embedded tissue. Fourteen of the 19 cases were classified as severe or established myoepithelial sialadenitis (MESA) and five were regarded as having mild MESA. The majority of intraepithelial lymphocytes were of B-cell lineage, while the pericystic infiltrate contained CD8-positive T-lymphocytes. p24 immunohistochemistry for HIV showed positive follicular dendritic cells, lymphoid cells and macrophages. Ten of 14 cases were positive for EBER 1 and 2. These included cases that were serologically negative for EBV. This study confirms that the morphology and immunophenotype of pediatric HIV-associated parotid lesions are similar to those seen in adults. Ten of 14 cases with evidence of EBV within the lymphoid infiltrate showed the same morphology and immunophenotype as cases in which EBV was not detected either by serology or by in situ hybridization. These findings indicate that EBV is not uniformly found in either the tissue or serum of these patients, and may not have a pathogenetic role in HIV-associated lymphoepithelial lesions in the pediatric age group.
authors.affiliation: Department of Pathology, University of Natal School of Medicine, Durban, South Africa. chettyr@med.und.ac.za
authors.forename: R
authors.initials: R
authors.lastname: Chetty
authors.forename: M
authors.initials: M
authors.lastname: Vaithilingum
authors.forename: R
authors.initials: R
authors.lastname: Thejpal
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D006679:HIV Seropositivity / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007223:Infant; D008297:Male; D010306:Parotid Gland / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D010309:Parotitis / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D012367:RNA, Viral / Q000378:metabolism; D012793:Sialadenitis / Q000378:metabolism / Q000473:pathology / Q000821:virology
chemical_list: C099537:Epstein-Barr virus encoded RNA 1; C106732:Epstein-Barr virus encoded RNA 2; D012367:RNA, Viral