DFFRY codes for a new human male-specific minor transplantation antigen involved in bone marrow graft rejection.
Graft rejection after histocompatibility locus antigen (HLA)-identical stem cell transplantation results from the recognition of minor histocompatibility antigens on donor stem cells by immunocompetent T lymphocytes of recipient origin. T-lymphocyte clones that specifically recognize H-Y epitopes on male target cells have been generated during graft rejection after sex-mismatched transplantation. Previously, 2 human H-Y epitopes derived from the same SMCY gene have been identified that were involved in bone marrow graft rejection. We report the identification of a new male-specific transplantation antigen encoded by the Y-chromosome-specific gene DFFRY. The DFFRY-derived peptide was recognized by an HLA-A1 restricted CTL clone, generated during graft rejection from a female patient with acute myeloid leukemia who rejected HLA-phenotypically identical bone marrow from her father. The identification of this gene demonstrates that at least 2 genes present on the human Y-chromosome code for male-specific transplantation antigens.
Vogt
M H
MH
Laboratory of Experimental Hematology, Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands.
de Paus
R A
RA
Voogt
P J
PJ
Willemze
R
R
Falkenburg
J H
JH
D000595:Amino Acid Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology*; D006182:H-Y Antigen / Q000235:genetics / Q000276:immunology*; D015788:HLA-A1 Antigen / Q000276:immunology; D006367:HeLa Cells; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics / Q000276:immunology / Q000628:therapy; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
D000939:Epitopes; D006182:H-Y Antigen; D015788:HLA-A1 Antigen; D006649:Histocompatibility Antigens; D010446:Peptide Fragments