High frequency recombination between homologous baculoviruses in cell culture.
The frequency of recombination between homologous baculoviruses was investigated in cell culture upon coinfection with two Autographa californica nucleopolyhedrovirus (AcMNPV) recombinants. These parental recombinants differed at two loci, separated by 20 kb, each carrying a different marker. The progeny recombinants generated by homologous recombination were easily distinguishable by the presence or absence of these markers. The mean percentage of the newly generated recombinants relative to a single locus varied between 23 and 41%, depending on the multiplicity of infection used for coinfection. These results provide further evidence that homologous recombination occurring during baculovirus replication is a highly frequent event.
Hajós
J P
JP
Department of Virology, Agricultural University Wageningen, Wageningen, The Netherlands.
Pijnenburg
J
J
Usmany
M
M
Zuidema
D
D
Závodszky
P
P
Vlak
J M
JM
D000818:Animals; D002460:Cell Line; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology*; D011995:Recombination, Genetic; D018411:Spodoptera; D010948:Viral Plaque Assay; D014776:Virus Cultivation