The complete cDNA sequence of a type II Trichomonas vaginalis virus.
Trichomonas vaginalis viruses (TVV), which may regulate P270 gene expression in the protozoan pathogen T. vaginalis, are a group of divergent double-stranded (ds) RNA viruses. In the present study, the complete 4674-bp cDNA sequence of a 4.6-kb ds RNA from a newly identified TVV2-1 isolate was determined. The sequence of the plus-strand mRNA contains four open reading frames, which encode overlapping cap and pol genes in the reading frame 2 and reading frame 1, respectively, and two putative serine-threonine-rich basic proteins VP3 and VP4 in the third reading frame. An 85-kDa capsid protein and a 160-kDa CAP-POL fusion protein were identified in crude viruses by Western blotting experiments using antisera raised against gene-specific oligopeptides. In conjunction with the presence of a potential ribosomal slippery heptanucleotide G GGC CCC within the overlap of the cap and pol genes, these observations suggest that the pol gene of TVV2-1 is translated via a -1 ribosomal frameshifting event during translation of the cap gene. Our results also provide insight into the conservation among divergent dsRNA species from TVV and suggest that the genome of TVV2-1 may encode two extra genes in addition to the cap and pol genes.
Bessarab
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Division of Infectious Diseases, Academia Sinica, Taiwan, Taipei, Republic of China.
Liu
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Ip
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Tai
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JH
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D016679:Genome, Viral; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014246:Trichomonas vaginalis / Q000821:virology*
D018076:DNA, Complementary; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases