Effects of a feedback-resistant aspartokinase III gene on L-isoleucine production in Escherichia coli K-12.
An L-isoleucine-overproducing recombinant strain of E. coli, TVD5, was also found to overproduce L-valine. The L-isoleucine productivity of TVD5 was markedly decreased by addition of L-lysine to the medium. Introduction of a gene encoding feedback-resistant aspartokinase III increased L-isoleucine productivity and decreased L-valine by-production. The resulting strain accumulated 12 g/l L-isoleucine from 40 g/l glucose, and suppression of L-isoleucine productivity by L-lysine was relieved.
Hashiguchi
K
K
Fermentation & Biotechnology Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan.
Matsui
H
H
Kurahashi
O
O
D001222:Aspartate Kinase / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D005246:Feedback; D007532:Isoleucine / Q000096:biosynthesis*; D007700:Kinetics; D010957:Plasmids; D011995:Recombination, Genetic; D014633:Valine / Q000096:biosynthesis
D007532:Isoleucine; D001222:Aspartate Kinase; D014633:Valine