title: Red blood cell phenotyping is a sensitive technique for monitoring chronic myeloid leukaemia patients after T-cell-depleted bone marrow transplantation and after donor leucocyte infusion.
abstract: Fifteen consecutive patients with Philadelphia chromosome (Ph)-positive chronic myeloid leukaemia (CML) who relapsed from T-cell-depleted bone marrow transplantation (BMT) were successfully treated with donor leucocyte infusions (DLIs). Chimaerism was analysed using red blood cell phenotyping (RCP), and the results were compared with cytogenetic analysis and outcome of qualitative and quantitative polymerase chain reaction (PCR) for breakpoint molecules. In all patients, an increase in autologous erythrocytes and/or a decrease in donor red cells indicated relapse. Donor erythrocytes started to increase from 4 to 20 (median 12) weeks after DLI. At 6 and 12 months after DLI, complete donor chimaerism was found in 11 and 15 patients, respectively, and all patients were in cytogenic remission. A high percentage of autologous red cells at the time of DLI predicted pancytopenia. During relapse and after DLI, the percentage of autologous red cells was strongly correlated with the reappearance and disappearance of Ph-positive metaphases (r = 0.90; P < 0.001 and r = 0.96; P < 0.001 respectively). The same was true for the correlation between the percentage of autologous red cells and positivity/negativity in PCR for Bcr-Abl breakpoint molecules (r = 0.94; P < 0.001). A normalized Bcr-Abl dose of greater than 10-3 in real-time quantitative PCR correlated well with relapse and the presence of autologous red blood cells (r = 0.77; P < 0.001). We conclude that RCP is a sensitive, easy to perform and fast technique for the prediction of pending relapse after BMT for CML. RCP also predicts the response to DLI and the occurrence of bone marrow aplasia after DLI.
authors.affiliation: Department of Haematology, University Hospital Nijmegen, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: Schaap
authors.forename: A
authors.initials: A
authors.lastname: Schattenberg
authors.forename: B
authors.initials: B
authors.lastname: Bär
authors.forename: E
authors.initials: E
authors.lastname: Mensink
authors.forename: A
authors.initials: A
authors.lastname: de Man
authors.forename: A
authors.initials: A
authors.lastname: Geurts van Kessel
authors.forename: T
authors.initials: T
authors.lastname: de Witte
mesh_terms: D000328:Adult; D000368:Aged; D016026:Bone Marrow Transplantation / Q000379:methods*; D004912:Erythrocytes / Q000502:physiology*; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D006086:Graft vs Host Disease / Q000209:etiology; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics / Q000628:therapy*; D017708:Leukocyte Transfusion / Q000379:methods; D008297:Male; D010198:Pancytopenia / Q000209:etiology; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012008:Recurrence; D018183:Transplantation Chimera / Q000235:genetics
chemical_list: D016044:Fusion Proteins, bcr-abl