Comparative FISH mapping on Z chromosomes of chicken and Japanese quail.
Using direct R-banding fluorescence in situ hybridization, we assigned five functional genes-growth hormone receptor (GHR), prolactin receptor (PRLR), spleen tyrosine kinase (SYK), aldolase B (ALDOB), and muscle skeletal receptor tyrosine kinase (MUSK)-to the chicken Z chromosome. SYK and MUSK were newly localized to the chicken Z chromosome in this study. GHR and PRLR were situated close to each other on the short arm of the chicken Z chromosome, as are their counterparts on human chromosome 5. SYK, MUSK, and ALDOB, which have been mapped to human chromosome 9, were localized to the long arm of the chicken Z chromosome. Thus, the present results indicate the presence of conserved synteny between the chicken Z chromosome and human chromosomes 5 and 9. Using the same method, four of the genes (GHR, PRLR, ALDOB, and MUSK) were assigned to the Japanese quail Z chromosome. The locations of these four Z-linked genes were conserved between chicken and Japanese quail. The results support the notion that the avian Z chromosome and the mammalian X chromosome did not evolve from a common ancestral linkage group.
Suzuki
T
T
Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Kansaku
N
N
Kurosaki
T
T
Shimada
K
K
Zadworny
D
D
Koide
M
M
Mano
T
T
Namikawa
T
T
Matsuda
Y
Y
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D003370:Coturnix / Q000235:genetics*; D019143:Evolution, Molecular; D005796:Genes / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D020161:Physical Chromosome Mapping; D017386:Sequence Homology, Amino Acid; D012730:Sex Chromosomes / Q000235:genetics*