Determination of edible bird's nest and its products by gas chromatography.
A specific gas chromatographic (GC) detection method for edible bird's nest (EBN) based on identifying the composition of the oligosaccharide chain combined with glycoprotein in EBN is developed. Five monoses (D-mannitose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, and N-acetyl neuraminate) that constitute the oligosaccharide chain are detected using GC and GC-mass spectrometry techniques; their characteristic GC spectrum can reliably be regarded as EBN's fingerprint. The peak-area ratios in GC spectrum of those five monoses are found to be fixed; therefore, the GC technique developed in this work can conveniently be used to determine various raw EBNs and their products both qualitatively and quantitatively, distinguishing between fake and genuine EBN rapidly.
Yu-Qin
Y
Y
Shanghai Food Industry Research Institute, China.
Liang
X
X
Hua
W
W
Hui-Xing
Z
Z
Xin-Fang
Z
Z
Bu-Sen
L
L
D000818:Animals; D001717:Birds; D002849:Chromatography, Gas / Q000379:methods*; D008519:Medicine, Traditional; D009844:Oligosaccharides / Q000032:analysis; D010945:Plants, Edible / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*
D009844:Oligosaccharides