Incorporation of simian virus 5 fusion protein into murine leukemia virus particles and its effect on the co-incorporation of retroviral envelope glycoproteins.
We describe the generation of murine leukemia virus (MLV) virus particles carrying the paramyxovirus fusion protein F from simian virus 5 (SV5-F). This glycoprotein was expressed in cells providing Moloney MLV (MoMLV) Gag and Pol proteins and a lacZ retroviral vector. SV5-F was correctly expressed, processed, and efficiently incorporated into retroviral particles. SV5-F-bearing retroviruses were not infectious although a weak binding to primate and rodent cells could be detected and SV5-F could mediate cell to cell fusion. We then co-expressed the SV5-F glycoprotein in retroviral particles with chimeric and wild-type MoMLV envelope glycoproteins. Our results show that F strongly inhibited infection via the retroviral envelopes although the mechanism of inhibition was different depending on the retroviral envelope used.
Hatziioannou
T
T
Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, Lyon Cedex 07, 69364, France.
Russell
S J
SJ
Cosset
F L
FL
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D015870:Gene Expression; D015686:Gene Products, env / Q000235:genetics; D005814:Genes, Viral; D008979:Moloney murine leukemia virus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D019213:Rubulavirus / Q000235:genetics*; D014162:Transfection; D014760:Viral Fusion Proteins / Q000235:genetics*
D015686:Gene Products, env; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins