Chemiluminescence of adzuki bean and soybean seedlings.
The chemiluminescence of extracts from leguminous seedlings (adzuki bean and soybean) was investigated. In an H(2)O(2)/gallic acid/water extract system, the photon intensities of adzuki bean seedlings were increased after germination and in the hypocotyls it reached a maximum level during the first 4 days of germination. On day 4 after germination, chemiluminescence in the primary leaf part exhibited the strongest intensity. Emission spectra showed a main peak at 510 nm, with shoulders at 660 nm. Mechanical injuries to the stems and cotyledons resulted in about a 1.5- and 6.8-fold increase of chemiluminescence, respectively. In an H(2)O(2)/70% EtOH extract/HRP system, photon intensities increased after germination and reached a maximum level during the first 2 days of germination. On day 4 after germination, chemiluminescence in the root and leaf area was stronger than in any other area. Emission spectra showed a main peak at around 570 nm, with shoulders at around 660 nm. The photon intensities of stems and cotyledons after mechanical injuries resulted in about an 0.72-fold decrease and an 8.8-fold increase in the presence of H(2)O(2) and acetaldehyde (MeCHO), respectively.
Iida
T
T
Department of Environmental Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori, Amamiya machi, Aoba-ku, Sendai, Miyagi 981-8555, Japan.
Kawane
M
M
Ashikaga
K
K
Yoshiki
Y
Y
Okubo
K
K
D007887:Fabaceae / Q000737:chemistry* / Q000254:growth & development; D018546:Hypocotyl / Q000737:chemistry; D008163:Luminescent Measurements; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal; D013025:Glycine max / Q000737:chemistry* / Q000254:growth & development
D010936:Plant Extracts