title: Broadening of coreceptor usage by human immunodeficiency virus type 2 does not correlate with increased pathogenicity in an in vivo model.
abstract: The pathogenic properties of four primary human immunodeficiency virus type 2 (HIV-2) isolates and two primary HIV-2 biological clones were studied in an in vivo human-to-mouse chimeric model. The cell-associated viral load and the ability to reduce the severity of the induced graft-versus-host disease symptoms, the CD4/CD8 ratio and the level of repopulation of the mouse tissues by the graft, were determined. All HIV-2 strains, irrespective of their in vitro biological phenotype, replicated to high titres and significantly reduced graft-versus-host disease symptoms as well as the CD4/CD8 ratios. Reduction of graft repopulation caused by infection with the respective HIV-2 strains showed that the in vitro replication rate, syncytium-inducing capacity and ability to infect human macrophages did influence the in vivo pathogenic potential whereas broadening of coreceptor usage did not.
authors.affiliation: Department of Virology, Erasmus Medical Centre Rotterdam, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.
authors.forename: M E
authors.initials: ME
authors.lastname: van Der Ende
authors.forename: C
authors.initials: C
authors.lastname: Guillon
authors.forename: P H
authors.initials: PH
authors.lastname: Boers
authors.forename: R A
authors.initials: RA
authors.lastname: Gruters
authors.forename: P
authors.initials: P
authors.lastname: Racz
authors.forename: K
authors.initials: K
authors.lastname: Tenner-Racz
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: M
authors.initials: M
authors.lastname: Schutten
mesh_terms: D000208:Acute Disease; D000818:Animals; D016516:CD4-CD8 Ratio; D002678:Chimera; D004195:Disease Models, Animal; D006086:Graft vs Host Disease / Q000276:immunology; D015658:HIV Infections / Q000209:etiology* / Q000276:immunology / Q000821:virology; D015498:HIV-2 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D051379:Mice; D015728:Receptors, HIV / Q000502:physiology*; D014183:Transplantation, Heterologous; D014779:Virus Replication
chemical_list: D015728:Receptors, HIV