GABA transporters (GAT-1) in Alzheimer's disease.
The presynaptically located gamma-aminobutyric acid (GABA) transporter (GAT-1) was studied in a group of patients with Alzheimer's disease (AD) and in a control group using the GAT-1 selective radioligand [3H]tiagabine. Post mortem brain tissue from frontal cortex, temporal cortex, and caudate nucleus from 18 AD patients and 23 age-matched controls were studied. The binding was saturable (Kd 26 nM) and region specific. There were no significant differences between the groups with respect to the binding capacity (Bmax) and binding affinity (Kd). The unaltered [3H]tiagabine binding to GAT-1 protein indicates that intrinsic GABA neurons are spared in Alzheimer's disease.
Nägga
K
K
Department of Geriatric Medicine, University Hospital of Linköping, Sweden. Katarina.Nagga@lio.se
Bogdanovic
N
N
Marcusson
J
J
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism*; D001923:Brain Chemistry; D002352:Carrier Proteins / Q000032:analysis*; D002421:Caudate Nucleus / Q000737:chemistry; D005260:Female; D005625:Frontal Lobe / Q000737:chemistry; D018755:GABA Agonists / Q000378:metabolism / Q000494:pharmacology; D050485:GABA Plasma Membrane Transport Proteins; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D026901:Membrane Transport Proteins; D016501:Neurites / Q000737:chemistry; D009557:Nipecotic Acids / Q000378:metabolism / Q000494:pharmacology; D027361:Organic Anion Transporters; D013702:Temporal Lobe / Q000737:chemistry; D000078308:Tiagabine; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002352:Carrier Proteins; D018755:GABA Agonists; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009557:Nipecotic Acids; D027361:Organic Anion Transporters; C493413:SLC6A1 protein, human; D014316:Tritium; D005680:gamma-Aminobutyric Acid; D000078308:Tiagabine