Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis.
A nonculture PCR-based method to characterize Neisseria meningitidis was used to test 225 clinical specimens. PCR correctly identified and predicted the serogroups of N. meningitidis of culture-proven meningococcal diseases and confirmed this diagnosis in 35% of suspected samples. This approach could be useful when culture fails to isolate N. meningitidis.
Taha
M K
MK
Unité des Neisseria and Centre National de Référence des Meningocoques, Institut Pasteur, 75724 Paris cedex 15, France. mktaha@pasteur.fr
D001769:Blood / Q000382:microbiology; D002555:Cerebrospinal Fluid / Q000382:microbiology; D003470:Culture Media; D006801:Humans; D008589:Meningococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping
D003470:Culture Media