Induction of protective immunity in chickens immunised with plasmid DNA encoding infectious bursal disease virus antigens.
Direct DNA inoculations were used to determine the efficacy of gene immunisation of chickens to elicit protective immune responses against infectious bursal disease virus (IBDV). The vp2 gene of IBDV strains GP40 and D78, and the vp2-vp4-vp3 encoding segment of strain D78 were cloned in an expression vector which consisted of human cytomegalovirus (HCMV) immediate early enhancer and promoter, adenovirus tripartite leader sequences and SV40 polyadenylation signal. For purification of vaccine-quality plasmid DNA from E. coli, an effective method was developed. Chickens were vaccinated by inoculation of DNA by two routes (intramuscular and intraperitoneal). Two weeks later, chickens were boosted with DNA, and at 2 weeks post-boost, they were challenged with virulent IBDV strain. Low to undetectable levels of IBDV-specific antibodies and no protection were observed with DNA encoding VP2. However, plasmids encoding VP2-VP4-VP3 induced IBDV-specific antibodies and protection in the chickens. DNA immunisation opens a new approach to the development of gene vaccines for chickens against infectious diseases.
Fodor
I
I
Agricultural Biotechnology Centre, Gödölló, Hungary. ifodor@som.llu.edu
Horváth
E
E
Fodor
N
N
Nagy
E
E
Rencendorsh
A
A
Vakharia
V N
VN
Dube
S K
SK
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control*; D002645:Chickens / Q000276:immunology; D004279:DNA, Viral / Q000737:chemistry; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular / Q000662:veterinary; D007274:Injections, Intraperitoneal / Q000662:veterinary; D010957:Plasmids / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology / Q000592:standards; D014765:Viral Vaccines / Q000276:immunology / Q000592:standards
D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines