An in vitro chemosensitivity test for colorectal cancer using collagen-gel droplet embedded cultures.
This study evaluated in vitro assay for chemosensitivity test using a collagen-gel droplet embedded culture drug sensitivity test (CD-DST) for colorectal cancer. CD-DST was performed in 24 patients with Dukes B, C colorectal cancer. Primary cultures of tumor cell samples from 87.5% (21/24) patients with colorectal cancer were successful. The efficacy rates assessed by CD-DST of five anticancer drugs evaluated were: 60.4% for adriamycin, 58.9% for etoposide, 56.7% for cisplatin, 53.4% for 5-fluorouracil, for 31.1% for mitomycin C, and 9.5% for vindesine. The present study demonstrated the clinical usefulness of CD-DST in evaluating the response to chemotherapy in colorectal cancer.
Araki
Y
Y
Department of Surgery, Kurume University Medical Center, Japan. kmc1@mocha.ocn.ne.jp
Isomoto
H
H
Matsumoto
A
A
Kaibara
A
A
Yasunaga
M
M
Hayashi
K
K
Yatsugi
H
H
Yamauchi
K
K
D000970:Antineoplastic Agents / Q000494:pharmacology*; D003094:Collagen; D015179:Colorectal Neoplasms / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D003094:Collagen