title: Distribution of Helicobacter pylori in a Mongolian gerbil gastric ulcer model.
abstract: OBJECTIVE
  To the authors' knowledge, histopathologic changes associated with early H. pylori infection and ulceration have not been established. We examined presence of H. pylori infection in an acetic acid-induced gastric ulcer (AAU) model in Mongolian gerbils.
  
  
  METHODS
  Sixty Mongolian gerbils were used as an AAU model, and another 60 gerbils were studied as a control (non-AAU) group. All animals were orally administered H. pylori, then were evaluated by use of histologic and bacteriologic examinations.
  
  
  RESULTS
  Helicobacter pylori were scattered on the surface mucous gel layer and in the pyloric gland gastric were pits; inflammation seen at the early stages later extended to the mucosa of the fundic gland area. The organisms were predominantly observed in the AAU model, but findings were comparable to those in controls at 1, 3, 7, 14, 28, or 56 days. Evaluation with regard to viable bacterial numbers reflected the histologic aspects, that the pyloric gland area had more viable counts than did the fundic gland area. Carbohydrate composition of mucin differed between pyloric and fundic gland areas. These findings shed light on L-fucose related to the H. pylori adhesive factor abundant in mucin of the pyloric gland area.
  
  
  CONCLUSIONS
  Findings for this ulcer model of Helicobacter pylori infection make it useful for the study of onset of infection and screening of anti-ulcer agents.
authors.affiliation: Research Center, Taisho Pharmaceutical Company, Ltd., Ohmiya-shi, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Miyata
authors.forename: K
authors.initials: K
authors.lastname: Yagi
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Kijima
authors.forename: Y
authors.initials: Y
authors.lastname: Isobe
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneda
authors.forename: T
authors.initials: T
authors.lastname: Akashi
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D005849:Gerbillinae / Q000382:microbiology*; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification / Q000472:pathogenicity*; D008297:Male; D013276:Stomach Ulcer / Q000139:chemically induced / Q000382:microbiology* / Q000473:pathology
chemical_list: D019342:Acetic Acid