[Interaction of 3'-fluoro-3'-deoxyanalogs of a trimer of (2'-5')oligoadenylic acid with (2'-5')A3-antibodies: stereochemical aspect].
Mouse antibodies to (2'-5')oligoadenylates were obtained by the immunization of animals with the (2'-5')oligoadenylic acid trimer conjugated with bovine serum albumin through a 2',3'-levulinic acid residue. Using radioimmunoassay, the reactivity of mouse polyclonal antibodies to the (2'-5')oligoadenylic acid trimer was studied for the trimer analogues containing 9-(3-deoxy-3-fluro-beta-D- xylofuranosyl)adenine and 3'-deoxy-3'-fluoro-adenosine in various positions of the chain. It was found that (a) the three-dimensional structure of short oligonucleotides is an important factor in the antibody recognition; (b) antibodies are more sensitive to modifications of the 5'-terminal and central ribose fragments of the (2'-5')oligoadenylic acid trimer; (c) the 3'-hydroxyl group plays a secondary role in the formation of the antigen determinant.
Kalinichenko
E N
EN
Institute of Bioorganic Chemistry, Belarussian Academy of Sciences, Minsk, Belarus.
Podkopaeva
T L
TL
Mikhaĭlopulo
I A
IA
Kelve
M
M
Saarma
M
M
van den Boogaart
J
J
Altona
C
C
D000227:Adenine Nucleotides / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D001704:Biopolymers; D002942:Circular Dichroism; D051379:Mice; D009843:Oligoribonucleotides / Q000737:chemistry / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013237:Stereoisomerism
D000227:Adenine Nucleotides; D000906:Antibodies; D001704:Biopolymers; D009843:Oligoribonucleotides; D012710:Serum Albumin, Bovine; C023505:2',5'-oligoadenylate