title: Transcription factor-dependent loading of the E1 initiator reveals modular assembly of the papillomavirus origin melting complex.
abstract: Replication of bovine papillomavirus type 1 DNA absolutely requires the viral transcription factor E2 as well as the initiator E1, although E1 alone has all the activities expected of an initiator protein. E1 assembles on the DNA in a stepwise fashion and undergoes a transition in activities from site-specific DNA-binding protein to mobile helicase. Complex assembly is assisted by the viral transcription factor E2 at two levels. E2 acts generally as a specificity factor, which through cooperative binding with E1 generates an initial E1 complex containing three E1 dimers bound to ori on one face of the DNA, E1-ori. Furthermore, E2 can promote the transition to an ori melting complex by recruiting additional E1 molecules to ori, effectively reducing the E1 concentration required for ori melting. This reaction is dependent on an E2-binding site positioned distal to the precursor E1-ori complex. The final origin melting complex has two subunits that each encircle the DNA and function independently to melt ori. The assembly pathway we describe has implication for understanding DNA melting and unwinding reactions, which are generally poorly understood.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Sanders
authors.forename: A
authors.initials: A
authors.lastname: Stenlund
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D052688:Bovine papillomavirus 1 / Q000502:physiology*; D002417:Cattle; D004265:DNA Helicases / Q000235:genetics; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C069814:E1 protein, Bovine papillomavirus; D014764:Viral Proteins; D004265:DNA Helicases