Enhanced production of penicillin V acylase from Streptomyces lavendulae.
A 28 degrees C, Streptomyces lavendulae produced high levels of penicillin V acylase (178 IU/l of culture) when grown on skim milk as the sole nutrient source for 275 h. The enzyme showed catabolite repression by glucose and was produced in the stationary phase of growth. Penicillin V was a good inducer of penicillin V acylase formation, while phenoxyacetic acid, the side-chain moiety of penicillin V, did not alter enzyme production significantly. The enzyme was stable between pH 6 and 11 and at temperatures from 20 degrees C to 55 degrees C. This extracellular enzyme was able to hydrolyse natural penicillins and unable to hydrolyse penicillin G.
Torres
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R
Departamento de Bioquímica y Biología Molecular I, Facultad de Biología, Universidad Complutense, Madrid, Spain.
Ramón
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F
de la Mata
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I
Acebal
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C
Castillón
M P
MP
D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000737:chemistry; D013302:Streptomyces / Q000201:enzymology*
D010399:Penicillin Amidase