title: Mitochondria-derived and extra-mitochondrial human type-1 porin are identical as revealed by amino acid sequencing and electrophysiological characterisation.
abstract: In mammalian cells porin channels are localised in both mitochondrial outer membranes and extra-mitochondrial membranes. We isolated mitochondria-derived porin of a human lymphoblastoid B cell line, determined its amino acid sequence and characterised its channel properties. Interestingly, the amino acid sequence of this porin preparation and, correspondingly, its electrophysiological characteristics in a reconstituted system were identical to those of 'Porin 31HL', the human type-1 porin purified from a crude membrane preparation of the same cell line using a different purification protocol. The results raise questions about targeting, insertion and orientation of human type-1 porin in different membranes.
authors.affiliation: Max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, Göttingen, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Stadtmüller
authors.forename: J
authors.initials: J
authors.lastname: Eben-Brunnen
authors.forename: A
authors.initials: A
authors.lastname: Schmid
authors.forename: D
authors.initials: D
authors.lastname: Hesse
authors.forename: S
authors.initials: S
authors.lastname: Klebert
authors.forename: H D
authors.initials: HD
authors.lastname: Kratzin
authors.forename: J
authors.initials: J
authors.lastname: Hesse
authors.forename: B
authors.initials: B
authors.lastname: Zimmermann
authors.forename: S
authors.initials: S
authors.lastname: Reymann
authors.forename: F P
authors.initials: FP
authors.lastname: Thinnes
authors.forename: R
authors.initials: R
authors.lastname: Benz
authors.forename: H
authors.initials: H
authors.lastname: Götz
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D018272:Porins / Q000737:chemistry* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D050995:Voltage-Dependent Anion Channel 1; D050994:Voltage-Dependent Anion Channels
chemical_list: D018272:Porins; C494762:VDAC1 protein, human; D050994:Voltage-Dependent Anion Channels; D050995:Voltage-Dependent Anion Channel 1