title: Phosphate inhibits acetotrophic methanogenesis on rice roots.
abstract: The contribution of acetate- and H(2)/CO(2)-dependent methanogenesis to total CH(4) production was determined in excised washed rice roots by radiolabeling, methyl fluoride inhibition, and stable carbon isotope fractionation. Addition of > or = 20 mM phosphate inhibited methanogenesis, which then was exclusively from H(2)/CO(2). Otherwise, acetate contributed about 50 to 60% of the total methanogenesis, demonstrating that phosphate specifically inhibited acetotrophic methanogens on rice roots.
authors.affiliation: Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany. conrad@mailer.uni-marburg.de
authors.forename: R
authors.initials: R
authors.lastname: Conrad
authors.forename: M
authors.initials: M
authors.lastname: Klose
authors.forename: P
authors.initials: P
authors.lastname: Claus
mesh_terms: D000085:Acetates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D019605:Euryarchaeota / Q000187:drug effects / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology*; D010710:Phosphates / Q000494:pharmacology*; D018517:Plant Roots / Q000382:microbiology*
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D010710:Phosphates; D002245:Carbon Dioxide; D006859:Hydrogen; D008697:Methane