title: The bulk of the phosphorylation of human respiratory syncytial virus phosphoprotein is not essential but modulates viral RNA transcription and replication.
abstract: The ability of variants of the human respiratory syncytial virus (HRSV) phosphoprotein (P protein) to support RNA transcription and replication has been studied by using HRSV-based subgenomic replicons. The serine residues normally phosphorylated in P during HRSV infection have been replaced by other residues. The results indicate that the bulk of phosphorylation of P (98%) is not essential for viral RNA transcription or replication but that phosphorylation can modulate these processes.
authors.affiliation: Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km2, Majadahonda, Madrid 28220, Spain. nvilla@isciii.es
authors.forename: N
authors.initials: N
authors.lastname: Villanueva
authors.forename: R
authors.initials: R
authors.lastname: Hardy
authors.forename: A
authors.initials: A
authors.lastname: Asenjo
authors.forename: Q
authors.initials: Q
authors.lastname: Yu
authors.forename: G
authors.initials: G
authors.lastname: Wertz
mesh_terms: D015707:HN Protein; D006801:Humans; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D012093:Replicon; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D015707:HN Protein; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G