Lucigenin luminescence elicited by microsomes and its modulation by nitroazole compounds.
The lucigenin luminescence elicited by rat liver microsomes and its modulation by the nitroazole compounds metronidazole and sanazole (drug AK-2123), as well as the rates of lucigenin-dependent NADPH consumption and cytochrome c reduction, were studied. The obtained data suggest that the luminescence can be the result of univalent lucigenin reduction by microsomal NAD(P)H-reductases, generation of superoxide anion radical in the redox cycle of lucigenin radicals, dioxetane formation by (di)oxygenases, and catalytic action of cytochrome P450 heme on dioxetane decomposition, followed by light emission.
Schepetkin
I A
IA
Institute of Oncology, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia. oncology@info.tsu.ru
D000166:Acridines / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D006736:Horses; D008163:Luminescent Measurements; D008297:Male; D008795:Metronidazole / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism
D000166:Acridines; D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D008795:Metronidazole; C033472:10,10'-dimethyl-9,9'-biacridinium; D009249:NADP; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D009247:NADH, NADPH Oxidoreductases