title: Mitochondrial distribution and function in herpes simplex virus-infected cells.
abstract: In this study, mitochondria migrated to a perinuclear region in the cytoplasm in herpes simplex virus (HSV)-infected cells. HSV infection did not promote the expression of cytochrome c oxidase subunit 2 but did promote that of stress-responsive HSP60, both of which are known to be components of mitochondria. The levels of cellular ATP and lactate and mitochondrial membrane potential were maintained for at least 6 h but decreased at the late stage of infection. It was also found that the UL41 and UL46 gene products, both of which are known to be tegument proteins, accumulated in the perinuclear region. The clustering of mitochondria and the accumulation of tegument proteins were completely blocked by the addition of nocodazole and vinblastine. These results suggest that mitochondria respond to the stimulation of HSV infection, migrating with tegument proteins along microtubules to a site around the nucleus, and maintain function until at least the middle stage of infection.
authors.affiliation: Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: F
authors.initials: F
authors.lastname: Goshima
authors.forename: T
authors.initials: T
authors.lastname: Daikoku
authors.forename: K
authors.initials: K
authors.lastname: Inagaki-Ohara
authors.forename: H
authors.initials: H
authors.lastname: Takakuwa
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D002460:Cell Line; D018834:Chaperonin 60 / Q000378:metabolism; D002522:Chlorocebus aethiops; D003576:Electron Transport Complex IV / Q000378:metabolism; D006561:Herpes Simplex / Q000473:pathology / Q000503:physiopathology; D018259:Herpesvirus 1, Human / Q000472:pathogenicity*; D018258:Herpesvirus 2, Human / Q000472:pathogenicity*; D006801:Humans; D018613:Microscopy, Confocal; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*; D009068:Movement / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D014709:Vero Cells; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D018834:Chaperonin 60; D014747:Vinblastine; D003576:Electron Transport Complex IV; D015739:Nocodazole