The visually-induced jump response of Drosophila melanogaster is sensitive to volatile anesthetics.
Fruit flies jump when startled by a sudden decrease in light intensity. We show that this visually-induced jump response in Drosophila melanogaster is sensitive to three general anesthetic agents: halothane, enflurane, and methoxyflurane. The concentration of anesthetic required to inhibit this response is similar to that needed for the inhibition of other assays in flies and other organisms. We believe the simplicity of this assay coupled with the insight gained from the more complex electrophysiological monitoring of the same neural pathway will be of value in the genetic identification of the molecular target of volatile anesthetics.
Campbell
J L
JL
Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892-4034, USA. campbell@codon.nih.gov
Nash
H A
HA
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D004737:Enflurane / Q000494:pharmacology; D005260:Female; D006221:Halothane / Q000494:pharmacology; D016015:Logistic Models; D008297:Male; D008733:Methoxyflurane / Q000494:pharmacology; D013216:Reflex, Startle / Q000187:drug effects / Q000235:genetics*
D018685:Anesthetics, Inhalation; D008733:Methoxyflurane; D004737:Enflurane; D006221:Halothane