Evaluation of miconazole activity contained in human serum to hypha of Aspergillus fumigatus.
Aspergillus fumigatus is an opportunistic pathogen, especially in an immunocompromised host. This fungus grows in a hyphal form in infected tissues; therefore, new tests to examine hyphal susceptibility are needed. In this study, we measured the mycotic activity of miconazole (MCZ) contained in human serum against A. fumigatus using the BioCell-Tracer method. Three serum samples were obtained from the same patient who was injected with 600 mg b.i.d. MCZ daily for 2 days. The concentrations of MCZ in the serum sample were 8.8, 3.5, and 1.6 micro g/ml, respectively. The serum containing 8.8 micro g/ml of MCZ inhibited hyphal growth 90 minutes after administration, and the hypha stopped growing. The serum containing 3.5 micro g/ml MCZ stopped hypha growth 100 minutes after administration, but re-growth of the hypha was observed at this concentration of MCZ. Serum containing 1.6 micro g/ml did not inhibite hyphal growth, nor did control serum have any inhibitory activity foward hyphae. Based on these results, we conclude that the BioCell-Tracer is a useful method for determining the effects on filamentous fungi of antifungal agents in the serum.
Taguchi
H
H
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University. 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
Miyaji
M
M
Yoshida
T
T
D000935:Antifungal Agents / Q000097:blood* / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D066298:In Vitro Techniques; D008825:Miconazole / Q000097:blood* / Q000494:pharmacology*
D000935:Antifungal Agents; D008825:Miconazole