title: New (15)N NMR exchange experiments for the unambiguous assignment of (1)H(N)/(15)N resonances of proteins in complexes in slow chemical exchange with free form.
abstract: The potentialities of a 2D proton-detected heteronuclear exchange experiment to assign the nitrogen and amide proton resonances in a uniformly (15)N-enriched macromolecule involved in a complex, starting from the free form assignments, are demonstrated on a protein-DNA complex. This 2D experiment is further extended to a 3D experiment in the case of severe superpositions.
authors.affiliation: Laboratoire de RMN, ICSN-CNRS, 1 Avenue de la Terrasse, Gif-sur-Yvette, F-91198, France.
authors.forename: C
authors.initials: C
authors.lastname: Vialle-Printems
authors.forename: C
authors.initials: C
authors.lastname: van Heijenoort
authors.forename: E
authors.initials: E
authors.lastname: Guittet
mesh_terms: D004247:DNA / Q000737:chemistry; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D011506:Proteins; D004247:DNA