title: Occurrence of phages infecting Escherichia coli O157:H7 carrying the Stx 2 gene in sewage from different countries.
abstract: Shiga toxin-converting bacteriophages are involved in the pathogenicity of some enteric bacteria, such as Escherichia coli O157:H7. Recent studies have demonstrated a relatively high presence of Shiga toxin 2 phages in sewage from Spain, but no data on sewage from other areas were available. In order to evaluate the presence of such phages in sewage from diverse geographical origins, 33 sewage samples, including samples from eight different European countries as well as from New Zealand and South Africa were analysed. Using an experimental approach based on the detection of Stx 2 gene by a phage enrichment culture followed by PCR, bacteriophages infecting E. coli O157:H7 carrying the Shiga toxin 2 gene were detected in 15 of the samples studied. Results presented here show that the presence of phages carrying the Stx 2 gene is common in sewage from developed countries.
authors.affiliation: Department of Microbiology, University of Barcelona, Diagonal 645, 08028, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Muniesa
authors.forename: J
authors.initials: J
authors.lastname: Jofre
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D003090:Coliphages / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D019049:Developed Countries; D019453:Escherichia coli O157 / Q000235:genetics / Q000821:virology*; D005060:Europe; D009520:New Zealand; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology / Q000821:virology*; D022603:Shiga Toxins; D013019:South Africa
chemical_list: D001427:Bacterial Toxins; D004279:DNA, Viral; D012722:Sewage; D022603:Shiga Toxins