title: Flow cytometric immunophenotypic characterization of pediatric and adult minimally differentiated acute myeloid leukemia (AML-M0).
abstract: We reviewed the clinicopathologic and immunophenotypic profiles of 7 pediatric and 11 adult minimally differentiated acute myelogenous leukemias (AML-M0). We also compared and evaluated myeloperoxidase in leukemic blasts using standard cytochemical and polyclonal antibody immunohistochemical stains. No distinctive clinical findings were noted in either patient group; however, thrombocytopenia typically was more prominent in adults. Adult AML-M0 also was associated with an immature myeloid profile (CD34+, terminal deoxynucleotidyl transferase positive, CD13+, and CD33+), in contrast with pediatric AML-M0, which usually lacked terminal deoxynucleotidyl transferase or CD34 but expressed bright CD33 with weak or negative CD13. Coexpression of the T-cell-associated antigen CD7 was observed in both groups. Antibody immunohistochemical stains were more sensitive than cytochemical stains for detection of myeloperoxidase activity and a useful adjunct for establishing a diagnosis of myeloid leukemia in paraffin-embedded marrow tissues.
authors.affiliation: Indiana University School of Medicine, Indianapolis, USA.
authors.forename: P K
authors.initials: PK
authors.lastname: Kotylo
authors.forename: I S
authors.initials: IS
authors.lastname: Seo
authors.forename: F O
authors.initials: FO
authors.lastname: Smith
authors.forename: N A
authors.initials: NA
authors.lastname: Heerema
authors.forename: N S
authors.initials: NS
authors.lastname: Fineberg
authors.forename: K
authors.initials: K
authors.lastname: Miller
authors.forename: M E
authors.initials: ME
authors.lastname: Greene
authors.forename: P
authors.initials: P
authors.lastname: Chou
authors.forename: A
authors.initials: A
authors.lastname: Orazi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D019011:Antigens, CD7 / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D018826:CD13 Antigens / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000032:analysis; D063268:Sialic Acid Binding Ig-like Lectin 3
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D019011:Antigens, CD7; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3; D009195:Peroxidase; D018826:CD13 Antigens