title: Phenotypic correction of feline lipoprotein lipase deficiency by adenoviral gene transfer.
abstract: Previous studies have revealed that adenovirus-mediated ectopic liver expression of human LPL (huLPL) can efficiently mediate plasma triacylglycerol (TG) catabolism in mice despite its native expression in adipose and muscle tissue. We aimed to explore the feasibility of liver-directed gene transfer and enzyme replacement for human LPL deficiency in a larger, naturally occurring feline animal model of complete LPL deficiency that is remarkably similar in phenotype to the human disorder. A cohort of LPL-deficient (LPL -/-) cats was given an intravenous injection of 8 x 10(9) PFU/kg of a CMV promoter/enhancer-driven, E1/E3-deleted adenoviral (Ad) vector containing a 1.36-kb huLPL cDNA (Ad-LPL) or reporter alkaline phosphatase gene (Ad-AP). After Ad-LPL administration, active, heparin-releasable huLPL was readily detected along with a 10-fold reduction in plasma TGs, disappearance of plasma TG-rich lipoproteins up to day 14, and enhanced clearance of an excess intravenous fat load on day 9. However, antibody against the huLPL protein was detected on day 14 in cats receiving Ad-LPL and adenovirus-specific neutralizing antibody was present 7 days after gene transfer in both cat cohorts. Tissue-specific expression of the huLPL transgene relative to controls was confirmed by RT-PCR. While huLPL expression was evident in the liver, other tissues including spleen and lung expressed huLPL message, in direct correlation with histological evidence of increased Oil red O (ORO)-positive neutral lipid influx. In contrast, intravenous LPL enzyme replacement therapy (ERT) led to rapid disappearance of 9000 mU/kg of active bovine LPL enzyme from the circulation, with t1/2 occurring at <10 min in two LPL-/- cats. Heparin injection 1 hr later released <10% of the original bovine LPL, further indicating its rapid systemic clearance, inactivation, or degradation as well as its ineffectiveness as a viable therapeutic alternative for complete LPL deficiency. Although LPL gene transfer and expression via this first-generation Ad vector was limited by the immune response against both the human LPL protein and adenovirus our results clearly provide a key advance supporting further development of LPL gene therapy as a viable therapeutic option for clinical LPL deficiency.
authors.affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: K J
authors.initials: KJ
authors.lastname: Ashbourne Excoffon
authors.forename: J E
authors.initials: JE
authors.lastname: Wilson
authors.forename: B M
authors.initials: BM
authors.lastname: McManus
authors.forename: Q R
authors.initials: QR
authors.lastname: Rogers
authors.forename: L
authors.initials: L
authors.lastname: Miao
authors.forename: J J
authors.initials: JJ
authors.lastname: Kastelein
authors.forename: M E
authors.initials: ME
authors.lastname: Lewis
authors.forename: M R
authors.initials: MR
authors.lastname: Hayden
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D002417:Cattle; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006720:Homozygote; D006801:Humans; D008071:Lipoprotein Lipase / Q000008:administration & dosage / Q000172:deficiency* / Q000235:genetics*; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008071:Lipoprotein Lipase