Strain difference in amiloride-sensitivity of salt-induced responses in mouse non-dissociated taste cells.
The chorda tympani nerve responses to NaCl in a mouse strain, C57BL/6 are known to be much more sensitive than those in BALB/c. We compared the NaCl-induced responses obtained from taste cells of the fungiform papillae in these two strains of mice. Amiloride inhibited, in the same degree, the responses induced by a bath-application of normal extracellular solution (NES) containing 140 mM NaCl in either taste cells of C57BL/6 and BALB/c mice. In contrast, amiloride inhibited 62% of responses induced by an apically applied 0.5 M NaCl in the C57BL/6 strain, but only 33% of responses in the BALB/c strain. These results suggest that the difference in amiloride-sensitivity between taste cells in both strains mainly derives from the difference in density of functional amiloride sensitive Na+ channels at the apical receptive membrane but not at the basolateral membrane.
Miyamoto
T
T
Department of Physiology, Nagasaki University School of Dentistry, Japan. miyamoto@net.nagasaki-u.ac.jp
Fujiyama
R
R
Okada
Y
Y
Sato
T
T
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D015222:Sodium Channels / Q000187:drug effects / Q000502:physiology; D012965:Sodium Chloride / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D013650:Taste Buds / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*
D015222:Sodium Channels; D012965:Sodium Chloride; D000584:Amiloride