title: Tissue expression and immunolocalization of a novel human cathepsin P.
abstract: The mRNA of a novel human cathepsin P is expressed at high levels in lung, liver and heart. Using antibodies raised against recombinant cathepsin P produced in Escherichia coli, a single protein band of 33 kDa was detected by immunoblotting an extract of human liver. By immunofluorescence, positive signals were observed in hepatocytes and Kupffer cells of liver, and the distal tubule cells of kidney showing mainly perimembranous distribution, indicating a role, as yet unknown, for this novel putative protease that is distinct from other cathepsins of the papain family.
authors.affiliation: Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Ljubljana, Slovenia. joze.pungercar@ijs.si
authors.forename: J
authors.initials: J
authors.lastname: Pungercar
authors.forename: A
authors.initials: A
authors.lastname: Viyjak
authors.forename: G
authors.initials: G
authors.lastname: Ivanovski
authors.forename: I
authors.initials: I
authors.lastname: Krizaj
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D056657:Cathepsin K; D002403:Cathepsins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D002403:Cathepsins; C534130:CTSK protein, human; D056657:Cathepsin K