title: Hereditary melanoblastoma in miniature pigs and its successful therapy by devitalization technique.
abstract: Applying selective breeding for 10 years we established the MeLiM (Melanoblastoma-bearing Libechov Minipigs) strain. Melanoblastoma (MB) in this strain shows a hereditary occurrence. Cutaneous tumours are usually nodular, multiple and distributed on various parts of body. They appear in darkly pigmented animals already at the birth or during two months thereafter (57% of all animals). Numerous organ metastases mainly into the spleen, lymph nodes and lungs are regularly ascertained in animals with cutaneous MB. Tumour cells were surprisingly found also in the inner organs of phenotypically healthy minipigs in which no cutaneous MBs were observed visually (27% of all animals). About 34% of all affected piglets die during the first 2 months of age. These features document a malignancy of this tumour in the MeLiM strain. Original surgical technique was applied in more than 40 affected minipigs at 1-2 months of age. It consists in a devitalization (ischemization) of one of cutaneous tumours by the mattress sutures conducted around the tumour base without any excision of tumour tissue. This simple procedure causes a total destruction of MB cells in all cutaneous tumours as well as in all organ metastases during 4-6 months. Animals treated by this technique were fully healed of tumour cells and no relapses were observed. This technique could bring similar positive results also in therapy of human MB.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov. horakv@iapg.cas.cz
authors.forename: V
authors.initials: V
authors.lastname: Horák
authors.forename: K
authors.initials: K
authors.lastname: Fortýn
authors.forename: V
authors.initials: V
authors.lastname: Hruban
authors.forename: J
authors.initials: J
authors.lastname: Klaudy
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D001947:Breeding; D005260:Female; D005799:Genes, Dominant; D005808:Genes, Recessive; D006200:Hair Color / Q000235:genetics; D008026:Ligation; D008175:Lung Neoplasms / Q000098:blood supply / Q000151:congenital / Q000235:genetics / Q000473:pathology / Q000601:surgery; D008285:Major Histocompatibility Complex / Q000235:genetics; D008297:Male; D008544:Melanocytes / Q000276:immunology; D008546:Melanoma, Experimental / Q000098:blood supply / Q000151:congenital / Q000235:genetics* / Q000276:immunology / Q000473:pathology / Q000601:surgery; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells / Q000473:pathology; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D009508:Nevus, Pigmented / Q000151:congenital / Q000235:genetics* / Q000473:pathology; D010641:Phenotype; D012075:Remission, Spontaneous; D012878:Skin Neoplasms / Q000098:blood supply / Q000151:congenital / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D012880:Skin Pigmentation / Q000235:genetics; D013160:Splenic Neoplasms / Q000098:blood supply / Q000151:congenital / Q000235:genetics / Q000473:pathology / Q000601:surgery; D013536:Suture Techniques; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics*; D014781:Viscera / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm