Acute inhibition of oestrogen biosynthesis does not affect serum leptin levels in young men.
OBJECTIVE
Leptin plays an important role in the regulation of reproduction. To explore the contribution of oestradiol to serum leptin levels in men, we measured the concentrations of serum leptin and insulin after inhibition of oestrogen biosynthesis by selective blockade of the aromatase enzyme.


METHODS
The study had a double-blind parallel group design.


METHODS
The aromatase inhibitor, MPV 2213ad, was given to eight healthy male volunteers as a single dose of 100mg. Eight men received placebo. Serum leptin and insulin were determined from blood samples collected at 0800h, 1600h and 2000h both on the actual test day (day 0) and on the previous day (day -1), and from single blood samples taken in the morning of days 1, 2, 4 and 7. Changes in serum leptin were correlated with those seen in serum oestradiol, testosterone, LH, FSH, cortisol and aldosterone, which were determined earlier.


RESULTS
After the aromatase inhibitor administration, mean serum oestradiol concentration was reduced by 74% from the baseline compared with a 19% reduction in the placebo group (P for difference <0.001), and returned to pre-treatment levels within four days. Despite marked changes in serum oestradiol and sustained elevations in serum testosterone, LH and FSH concentrations, serum leptin concentrations were similar in the group receiving the aromatase inhibitor and in the placebo group. We found a weak correlation between serum oestradiol and leptin, which could not be reproduced when the percentage changes in these variables were analysed.


CONCLUSIONS
Marked short-term reduction in serum oestradiol concentration has no effect on serum leptin levels in young men.
Luukkaa
V
V
Department of Pharmacology, University of Turku, and Clinical Pharmacology Unit, Turku University Central Hospital, FIN-20520 Turku, Finland. virve.luukkaa@utu.fi
Rouru
J
J
Ahokoski
O
O
Scheinin
H
H
Irjala
K
K
Huupponen
R
R
D000328:Adult; D047072:Aromatase Inhibitors; D004311:Double-Blind Method; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D004967:Estrogens / Q000096:biosynthesis*; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D020738:Leptin / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009570:Nitriles / Q000494:pharmacology; D009994:Osmolar Concentration; D013739:Testosterone / Q000097:blood; D013997:Time Factors; D014230:Triazoles / Q000494:pharmacology
D047072:Aromatase Inhibitors; D004791:Enzyme Inhibitors; D004967:Estrogens; D020738:Leptin; D009570:Nitriles; D014230:Triazoles; D013739:Testosterone; C111409:finrozole; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone