Gene expression of peroxisomal beta-oxidation enzymes in rat brain.
Despite increasing insight into peroxisomal beta-oxidation, it is still not clear which enzymes catalyze very-long-chain fatty acid degradation. Using the northern blot and RT-PCR techniques, a brain-specific expression is demonstrated for acyl-CoA oxidase 3II mRNA, thiolase-A and trans2,3enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase multifunctional enzyme type 2.
Knoll
A
A
Laboratoire de Biogenèse Membranaire, CNRS-UMR 5544, Université Victor Segalen Bordeaux 2, 146, Rue Léo Saignat Case 92, 33076, Bordeaux, France. anja.knoll@biomemb.u-bordeaux2.fr
Sargueil
F
F
Salles
J
J
Cassagne
C
C
Garbay
B
B
D015067:17-Hydroxysteroid Dehydrogenases; D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics; D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics; D042962:Acyl-CoA Oxidase; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D002352:Carrier Proteins / Q000235:genetics; D004746:Enoyl-CoA Hydratase; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006836:Hydro-Lyases / Q000235:genetics; D009097:Multienzyme Complexes / Q000235:genetics; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics; D064029:Peroxisomal Multifunctional Protein-2; D020675:Peroxisomes / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
D002352:Carrier Proteins; D004798:Enzymes; D009097:Multienzyme Complexes; D012333:RNA, Messenger; C072087:sterol carrier proteins; D010088:Oxidoreductases; D015067:17-Hydroxysteroid Dehydrogenases; D015094:3-Hydroxyacyl CoA Dehydrogenases; C491286:Hsd17b4 protein, rat; D042962:Acyl-CoA Oxidase; D000101:Acetyl-CoA C-Acetyltransferase; D006836:Hydro-Lyases; D064029:Peroxisomal Multifunctional Protein-2; D004746:Enoyl-CoA Hydratase