title: Warthin's tumour is not an Epstein-Barr virus related disease.
abstract: BACKGROUND
  The histogenesis of Warthin's tumour (WT) is controversial. A possible role for Epstein-Barr virus (EBV) has been suggested.
  
  
  METHODS
  Twenty formaldehyde-fixed, paraffin-embedded blocks of WT from the parotid gland were examined for the presence of EBV. In situ hybridisation was performed using EBV encoded small nuclear RNAs (EBER1/2) probes labelled with fluorescein isothiocyanate. An EBV-positive P3HR-1 cell line processed to paraffin wax was used as a positive control and a brain section as negative control.
  
  
  RESULTS
  EBER1/2 could not be found in the neoplastic epithelial cells in any of the tumours nor in the adjacent normal parotid tissues. Individual positive lymphocytes were present in 7 tumours.
  
  
  CONCLUSIONS
  These results indicated that EBV is not involved in the pathogenesis of WT.
authors.affiliation: Department of Oral Pathology and Oral Biology, University of Pretoria, South Africa.
authors.forename: W F
authors.initials: WF
authors.lastname: van Heerden
authors.forename: K
authors.initials: K
authors.lastname: Kraft
authors.forename: J
authors.initials: J
authors.lastname: Hemmer
authors.forename: T J
authors.initials: TJ
authors.lastname: Swart
authors.forename: M B
authors.initials: MB
authors.lastname: van Heerden
authors.forename: E J
authors.initials: EJ
authors.lastname: van Rensburg
mesh_terms: D000235:Adenolymphoma / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral
chemical_list: D012367:RNA, Viral