Biological activities of lipopolysaccharides extracted from porcine vaccine strains.
Lipopolysaccharides (LPSs) were purified from Actinobacillus pleuropneumoniae serotype 2, Bordetella bronchiseptica and Haemophilus parasuis serotype 5, which were used for vaccine production in Japan, by the phenol-water procedure. In SDS-PAGE analysis, A. pleuropneumoniae LPS, as well as Escherichia coli LPS, demonstrated a typical ladder profile of a smooth-type LPS. On the other hand, B. bronchiseptica and H. parasuis LPSs lacked the ladder profiles. It was found that the biological activity of these LPSs was comparable to those of E. coli LPS in terms of activation of the clotting enzyme of Limulus amoebocyte lysate, mitogenic activity of mouse spleen cells, stimulation of TNF-alpha and nitric oxide production, but IL-6 production could hardly be observed in any LPS.
Ogikubo
Y
Y
National Veterinary Assay Laboratory, Tokyo, Japan.
Norimatsu
M
M
Kojima
A
A
Sasaki
Y
Y
Tamura
Y
Y
D000189:Actinobacillus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D016977:Actinobacillus pleuropneumoniae / Q000276:immunology; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology* / Q000592:standards; D001885:Bordetella Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D016950:Bordetella bronchiseptica / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005562:Formazans / Q000737:chemistry; D006190:Haemophilus / Q000276:immunology; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D018073:Haemophilus Vaccines / Q000276:immunology / Q000592:standards; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis; D007928:Lethal Dose 50; D008033:Limulus Test / Q000662:veterinary; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology* / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D013778:Tetrazolium Salts / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis; D014611:Vaccination / Q000662:veterinary*
D001428:Bacterial Vaccines; D005562:Formazans; D018073:Haemophilus Vaccines; D015850:Interleukin-6; D008070:Lipopolysaccharides; D013778:Tetrazolium Salts; D014409:Tumor Necrosis Factor-alpha; C079782:MTT formazan; D009569:Nitric Oxide