title: A light and electron microscopic study of GAT-1-positive cells in the cerebral cortex of man and monkey.
abstract: Specimens of human cerebral cortex were obtained during neurosurgical operations and studied by immunocytochemistry and electron microscopy, using antibodies to the GABA transporter GAT-1. Cortical material from macaque monkeys was prepared similarly. Large numbers of GAT-1-positive non-pyramidal neurons were observed in layers I, II, V, and VI of the cortex. Electron microscopy also showed that the GAT-1-positive axon terminals formed symmetrical and not asymmetrical synapses, suggesting that they were the terminals of non-pyramidal neurons. Processes of cells in the walls of blood vessels were also labelled. We conclude that GAT-1 is present in cell bodies and axon terminals of non-pyramidal neurons, and a population of mural cells in blood vessels, in the primate cerebral cortex.
authors.affiliation: Department of Anatomy, National University of Singapore, Singapore.
authors.forename: W Y
authors.initials: WY
authors.lastname: Ong
authors.forename: T T
authors.initials: TT
authors.lastname: Yeo
authors.forename: V J
authors.initials: VJ
authors.lastname: Balcar
authors.forename: L J
authors.initials: LJ
authors.lastname: Garey
mesh_terms: D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D005625:Frontal Lobe / Q000166:cytology*; D050485:GABA Plasma Membrane Transport Proteins; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D026901:Membrane Transport Proteins; D008854:Microscopy, Electron; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D027361:Organic Anion Transporters; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D013569:Synapses / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; C493413:SLC6A1 protein, human; C493415:Slc6a1 protein, rat