title: Acidic amino acid clearance from CSF in the neonatal versus adult rat using ventriculo-cisternal perfusion.
abstract: The acidic amino acids aspartate and glutamate are excitatory neurotransmitters in the CNS. The clearance of this group of amino acids from CSF of adult and neonatal (7-day-old) rats was investigated. Ventriculo-cisternal perfusions with 14C-amino acids and 3H-dextran were carried out for up to 90 min. Uptake of the amino acids by the whole brain was measured, and the loss to blood was calculated. 3H-Dextran was included in the perfusate for measurement of CSF secretion rate. After 90-min perfusion, both aspartate and glutamate showed a similar uptake into the whole brain, and this did not change with age (p>0.05). However, clearance from CSF was greater in the adult, as was entry into blood from CSF. Addition of 5 mM excess unlabelled amino acid resulted in reduction in the brain uptake of both 14C-amino acids in the adult rat. In the neonate, addition of aspartate also reduced brain aspartate uptake, whereas addition of glutamate increased brain neonatal [14C]glutamate uptake. The rate of CSF secretion was significantly greater in the adult, 1.26+/-0.18 microl x min(-1) x g(-1), than in the neonate, 0.62+/-0.08 microl x min(-1) x g(-1), and the turnover of CSF was greater in adults (p<0.01). In summary, both aspartate and glutamate showed greater clearances from CSF in the adult than the neonate. This clearance was found to be by carrier-mediated mechanisms.
authors.affiliation: Division of Physiology, St. Thomas' Hospital Campus, London, England. hameed@hsc.kuniv.edu.kw
authors.forename: H
authors.initials: H
authors.lastname: Al-Sarraf
authors.forename: J E
authors.initials: JE
authors.lastname: Preston
authors.forename: M B
authors.initials: MB
authors.lastname: Segal
mesh_terms: D000375:Aging / Q000134:cerebrospinal fluid*; D000596:Amino Acids / Q000097:blood / Q000134:cerebrospinal fluid* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000097:blood / Q000134:cerebrospinal fluid* / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002552:Cerebral Ventricles; D002555:Cerebrospinal Fluid / Q000502:physiology; D002946:Cisterna Magna; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids