Detection of adenovirus hexon sequence in a cat by polymerase chain reaction (short communication).
Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in pharyngeal and rectal swab samples of a cat seropositive for adenovirus and suffering from transient hepatic failure. The samples were taken at a one-year interval, and both faecal samples as well as the second pharyngeal sample were positive in PCR performed with general adenovirus primers. The size of the amplified products corresponded to that of the positive control. The identity of the amplicons was also confirmed by DNA sequencing. The 301 bp long hexon gene fragment was very similar to but distinguishable from the corresponding hexon sequence of human adenovirus type 2. This result suggests the possibility of persistent carrier status and shedding of adenovirus in cats.
Lakatos
B
B
University of Veterinary Sciences, Budapest, Hungary. lakbel@net.sote.hu
Farkas
J
J
Egberink
H F
HF
Vennema
H
H
Horzinek
M C
MC
Benkó
M
M
D000256:Adenoviridae / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D002371:Cat Diseases / Q000821:virology*; D002415:Cats; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004279:DNA, Viral