title: Subpopulations of rat B2(+) neuroblasts exhibit differential neurotrophin responsiveness during sympathetic development.
abstract: Sympathetic neurons comprise a population of postmitotic, tyrosine hydroxylase expressing cells whose survival is dependent upon nerve growth factor (NGF) both in vivo and in vitro. However, during development precursors to rat sympathetic neurons in the thoracolumbar region are not responsive to NGF because they lack the signal transducing NGF receptor, trkA. We have previously shown that acquisition of trkA expression is sufficient to confer a functional response to NGF. Here we describe four subpopulations of thoracolumbar sympathetic neuroblasts which are mitotically active and unresponsive to NGF at E13.5 of rat gestation, but differ based upon their neurotrophic responsiveness in vitro. The survival in culture of the largest sympathetic subpopulation is mediated by neurotrophin-3 (NT-3) or glial-derived neurotrophic factor (GDNF), whereas the cell survival of two smaller subpopulations of neuroblasts are mediated by either solely GDNF or solely NT-3. Finally, we identify a subpopulation of sympathetic neuroblasts in the thoracolumbar region whose survival, exit from the cell cycle, induction of trkA expression, and consequent acquisition of NGF responsiveness in culture appear to be neurotrophin independent and cell autonomous. These subpopulations reflect the diversity of neurotrophic actions that occur in the proper development of sympathetic neurons.
authors.affiliation: Neurodegeneration Research Group, John P. Robarts Research Institute, London, Ontario, N6A 5K8, Canada.
authors.forename: D E
authors.initials: DE
authors.lastname: Goldhawk
authors.forename: S O
authors.initials: SO
authors.lastname: Meakin
authors.forename: J M
authors.initials: JM
authors.lastname: Verdi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development; D009414:Nerve Growth Factors / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D013564:Sympathetic Nervous System / Q000166:cytology / Q000196:embryology*
chemical_list: D009414:Nerve Growth Factors