6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase expression in rat brain during development.
This study reports the expression of the ubiquitous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene (PFKFB3) (PFK-2/FBPase-2) in different stages of rat brain development. Northern blot and RT-PCR analysis demonstrated that ubiquitous PFK-2/FBPase-2 is expressed in rat brain from embryonic to adult life and shows a transient increase 1 day before birth, coincident with the maximum concentration of Fru-2,6-P(2) and PFK-2 activity. The levels of brain PFK-2/FBPase-2 gene expression as well as the enzymatic activity and the concentration of Fru-2,6-P(2) appear to be remarkably constant during adult life, without significant differences in the brain hippocampus, cortex, cerebellum or striatum areas.
Goren
N
N
Cátedra de Farmacología, Facultad de Odontología, Universidad de Buenos Aires, CEFYBO-CONICET, Buenos Aires, Argentina.
Manzano
A
A
Riera
L
L
Ambrosio
S
S
Ventura
F
F
Bartrons
R
R
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D005865:Gestational Age; D008297:Male; D009097:Multienzyme Complexes / Q000235:genetics*; D010732:Phosphofructokinase-1 / Q000235:genetics*; D025481:Phosphofructokinase-2; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009097:Multienzyme Complexes; D025481:Phosphofructokinase-2; D010732:Phosphofructokinase-1; D006597:Fructose-Bisphosphatase