Chemical modification of silk fibroin with 2-methacryloyloxyethyl phosphorylcholine. II. Graft-polymerization onto fabric through 2-methacryloyloxyethyl isocyanate and interaction between fabric and platelets.
2-Methacryloyloxyethyl phosphorylcholine (MPC) was grafted onto silk fabric in a two-step heterogeneous system through the vinyl bonds of 2-methacryloyloxyethyl isocyanate (MOI) modified on the fabric. First, habutae silk fabric was modified with the MOI monomer in anhydrous dimethyl sulfoxide using di-n-butyltin (IV) dilaurate and hydroquinone at 35 degrees C. The saturated weight gain of modified MOI monomer on the fabric was 7.3 wt% versus the original silk. Second, graft polymerization with MPC onto the MOI modified silk was conducted using 2,2'-azo bis[2-(2-imidazolin-2-yl)propane dihydrochloride] (VA-044) as an azo polymerization initiator. The weight of the grafted MPC eventually gained was about 26.0 wt%. The MOI-modified and MPC-grafted silk fabrics were analyzed by Fourier transform infrared (FT-IR) spectroscopy. To confirm the improved biocompatibility of the silk fabric, platelet adhesion was preliminarily tested measuring lactate dehydrogenase. The number of platelets adhering to polyMPC-grafted silk fabric decreased by about one tenth compared to original and MOI-modified silk after 60 min of contact with human platelet-rich plasma (1.0 x 10(6) platelets cm(-2)).
Furuzono
T
T
National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan.
Ishihara
K
K
Nakabayashi
N
N
Tamada
Y
Y
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D012831:Bombyx; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry* / Q000494:pharmacology; D017953:Isocyanates / Q000737:chemistry* / Q000494:pharmacology; D008689:Methacrylates / Q000737:chemistry* / Q000494:pharmacology; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011109:Polymethacrylic Acids; D047011:Silk; D017550:Spectroscopy, Fourier Transform Infrared; D013784:Textiles
D001672:Biocompatible Materials; D019476:Insect Proteins; D017953:Isocyanates; D008689:Methacrylates; D011109:Polymethacrylic Acids; D047011:Silk; C115766:poly(2-methacryloyloxyethyl-phosphorylcholine); D010767:Phosphorylcholine; C029845:2-isocyanatoethyl methacrylate; D005349:Fibroins