Replication of Aleutian mink disease parvovirus in mink lymph node histocultures.
Aleutian mink disease parvovirus (ADV), causes an immune disorder with a persistent infection of lymphoid organs in adult mink. We studied replication of ADV in gel-supported histocultures prepared from adult mink mesenteric lymph node (MLN). Evidence of virus replication in the histocultures was first observed by indirect immunofluorescence 72 h after incubation with virus. Cells resembling lymphocytes and macrophages contained both ADV capsid (VP2) and nonstructural (NS1 and NS2) proteins, and were present in a distribution suggestive of infected cells within germinal centres. ADV replicative form and encapsidated virion DNA were also detected in infected histocultures at time-points after 72 h. In addition, we were able to passage ADV-Utah to a new round of histocultures. These results suggested that the infected cells were actual target cells for ADV replication and that productive ADV-Utah replication, complete with the generation of virus, was occurring in the histocultures. The mink MLN histocultures provide a system to study the replication and molecular pathogenesis of ADV in target tissues.
Jensen
K T
KT
Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT 59840, USA.
Wolfinbarger
J B
JB
Aasted
B
B
Bloom
M E
ME
D000453:Aleutian Mink Disease / Q000209:etiology / Q000821:virology; D000454:Aleutian Mink Disease Virus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D046508:Culture Techniques; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D019084:Fluorescent Antibody Technique, Indirect; D008198:Lymph Nodes / Q000821:virology; D008907:Mink; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral