title: Oxidative DNA damage by vitamin A and its derivative via superoxide generation.
abstract: Recent intervention studies revealed that beta-carotene supplement to smokers resulted in a higher incidence of lung cancer. However, the causal mechanisms remain to be clarified. We reported here that vitamin A (retinol) and its derivative (retinal) caused cellular DNA cleavage detected by pulsed field gel electrophoresis. Retinol and retinal significantly induced 8-oxo-7,8-dihydro-2'-deoxyguanosine formation in HL-60 cells but not in H(2)O(2)-resistant HP100 cells, suggesting the involvement of H(2)O(2) in cellular DNA damage. Experiments using (32)P-labeled isolated DNA demonstrated that retinol and retinal caused Cu(II)-mediated DNA damage, which was inhibited by catalase. UV-visible spectroscopic and electron spin resonance-trapping studies revealed the generation of superoxide and carbon-centered radicals, respectively. The superoxide generation during autoxidation of retinoids was significantly correlated with the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine, although the yield of carbon-centered radicals was not necessarily related to the intensity of DNA damage. These findings suggest that superoxide generated by autoxidation of retinoids was dismutated to H(2)O(2), which was responsible for DNA damage in the presence of endogenous metals. Retinol and retinal have prooxidant abilities, which might lead to carcinogenesis of the supplements of beta-carotene.
authors.affiliation: Department of Hygiene, Mie University School of Medicine, Tsu, Mie, 514-8507, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Kawanishi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002417:Cattle; D002460:Cell Line; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D016521:Electrophoresis, Gel, Pulsed-Field; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D016158:Genes, p53 / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D010618:Phenanthrolines / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012172:Retinaldehyde / Q000633:toxicity*; D013379:Substrate Specificity; D013481:Superoxides / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism; D013997:Time Factors; D014801:Vitamin A / Q000633:toxicity*
chemical_list: D016166:Free Radical Scavengers; D005609:Free Radicals; D010618:Phenanthrolines; D017382:Reactive Oxygen Species; D013481:Superoxides; D014801:Vitamin A; D000080242:8-Hydroxy-2'-Deoxyguanosine; C002478:bathocuproine; D006861:Hydrogen Peroxide; D004262:DNA Restriction Enzymes; D003849:Deoxyguanosine; D012172:Retinaldehyde