High-molecular-weight kininogen preadsorbed to glass surface markedly reduces neutrophil adhesion.
Adsorbed proteins on biomaterial surfaces determine whether cells adhere, but rheological variables are also critical. Neutrophil adhesion under well-defined radial flow conditions was studied on glass preadsorbed with plasma proteins or plasma protein domain fragments. Fibrinogen, low-molecular-weight kininogen (LK), high-molecular-weight kininogen (HK), cleaved HK (HKa), and recombinant HK domains 3 and 5 (D3 and D5H) were used. The number of adherent cells on the HK and HKa surfaces was less than 10% that found on the fibrinogen absorbed surface. The degree of spreading was minimal and detachment of adherent neutrophils was observed. HK and HKa contain binding sites for both anionic surfaces and neutrophils in the same domain (D5H). When adsorbed to surfaces, HK and HKa did not have the neutrophil binding sites available and therefore exhibited an anti-adhesive effect. Although D5H contains anionic surface binding sites, its small molecular size required a higher number of adsorbed molecules to cover the surface before a significant decrease in cell adhesion was observed. Since LK and D3 do not possess specific anionic surface binding sites, the adsorption of these proteins on glass was very low compared to HK and HKa. Thus, extensive cell adhesion and spreading were observed on the surfaces partially covered with preadsorbed LK and D3.
Yung
L Y
LY
Department of Chemical Engineering, University of Delaware, Newark 19716, USA. yung@che.udel.edu
Lim
F
F
Khan
M M
MM
Kunapuli
S P
SP
Rick
L
L
Colman
R W
RW
Cooper
S L
SL
D000327:Adsorption; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005898:Glass; D006801:Humans; D019679:Kininogen, High-Molecular-Weight / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*; D019728:Kininogen, Low-Molecular-Weight / Q000097:blood / Q000737:chemistry / Q000494:pharmacology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000097:blood / Q000737:chemistry / Q000494:pharmacology; D012212:Rheology; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D019679:Kininogen, High-Molecular-Weight; D019728:Kininogen, Low-Molecular-Weight; D011994:Recombinant Proteins; D005340:Fibrinogen