TH-, NPY-, SP-, and CGRP-immunoreactive nerves in interscapular brown adipose tissue of adult rats acclimated at different temperatures: an immunohistochemical study.
Interscapular brown adipose tissue (IBAT), a site of nonshivering thermogenesis in mammals, is neurally controlled. The co-existence of sympathetic and peptidergic innervation has been demonstrated in different brown adipose depots. We studied the morphological profile of IBAT innervation and tested by immunohistochemical methods whether cold and warm stimulation are accompanied by modifications in the density of parenchymal noradrenergic nerve fibers. We also studied the immunoreactivity of afferent fibers--which contain calcitonin gene-related peptide (CGRP) and substance P (SP)--in different functional conditions. IBAT was obtained from adult rats (6 weeks old) acclimated at different temperatures (4 degrees, 20 degrees, and 28 degrees C). Tissue activity was evaluated by studying the immunolocalization of uncoupling protein (UCP-1), a specific marker of brown adipose tissue. Noradrenergic and peptidergic innervation were seen to arise from morphologically different nerves. Fibers staining for tyrosine hydroxylase (TH) were thin, unmyelinated hilar nerves, and CGRP- and SP-positive fibers were in thick nerves containing both myelinated and unmyelinated fibers. Under cold stimulation, noradrenergic neurons produce greater amounts of TH, and their axons branch, resulting in increased parenchymal nerve fibers density. Neuropeptide Y (NPY) probably co-localizes with TH in noradrenergic neurons, but only in the perivascular nerve fiber network. The parenchymal distribution of NPY to interlobular arterioles and capillaries suggests that this peptide must have other functions besides that of innervating arteriovenous anastomoses, as hypothesized by other researchers. The different distribution of CGRP and SP suggests the existence of different sensory neuronal populations. The detection of CGRP at the parenchymal level is in line with the hypothesis of a trophic action of this peptide.
De Matteis
R
R
Institute of Normal Human Morphology-Anatomy, University of Ancona, Italy.
Ricquier
D
D
Cinti
S
S
D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000294:innervation*; D000818:Animals; D001833:Body Temperature Regulation; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis; D003238:Connective Tissue / Q000294:innervation; D003239:Connective Tissue Cells / Q000166:cytology; D007150:Immunohistochemistry; D007473:Ion Channels; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D024101:Mitochondrial Proteins; D009478:Neuropeptide Y / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000032:analysis*; D013696:Temperature; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009478:Neuropeptide Y; C000604371:UCP1 protein, human; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D013373:Substance P; D014446:Tyrosine 3-Monooxygenase; D015740:Calcitonin Gene-Related Peptide