Integrating microfabricated fluidic systems and NMR spectroscopy.
The philosophy of miniature total analysis systems (mu-TAS) hinges on the integration of multiple chemical processing steps and the means of analyzing their results on the same miniaturized system. We have constructed chip-based capillary electrophoresis (CE) devices equipped with an integrated planar radio-frequency detector coil used for nuclear magnetic resonance spectroscopy (NMR). Separations were accomplished in the devices, but satisfactory NMR spectra could only be obtained from samples of high concentration. The relative sensitivity is explained and the scaling law dichotomy of CE and NMR explored.
Trumbull
J D
JD
Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign 61801, USA.
Glasgow
I K
IK
Beebe
D J
DJ
Magin
R L
RL
D019075:Electrophoresis, Capillary; D004867:Equipment Design; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation*; D012680:Sensitivity and Specificity