title: Indigenous plasmids in a production line of strains for penicillin G acylase derived from Escherichia coli W.
abstract: Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the strain RE3 without plasmid pRK1 and the plasmid-less strain cERE3 were isolated. Indigenous plasmids did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.
authors.affiliation: Laboratory of Enzyme Technology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Sobotková
authors.forename: J
authors.initials: J
authors.lastname: Grafková
authors.forename: V
authors.initials: V
authors.lastname: Stĕpánek
authors.forename: T
authors.initials: T
authors.lastname: Vacík
authors.forename: H
authors.initials: H
authors.lastname: Maresová
authors.forename: P
authors.initials: P
authors.lastname: Kyslík
mesh_terms: D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D007218:Industrial Microbiology; D008826:Microbial Sensitivity Tests; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000235:genetics; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; D010399:Penicillin Amidase