14-3-3zeta interacts with the alpha-chain of human interleukin 9 receptor.
Interleukin 9 (IL-9) exerts its pleiotropic effects through the IL-9 receptor (IL-9R) complex, which consists of the IL-9R alpha-chain, which determines the cytokine specificity, and the IL-2 receptor gamma-chain. In the present study we used a modified yeast two-hybrid system to isolate cDNA species encoding proteins that interacted with the intracellular domain of the human IL-9R alpha-chain (hIL-9Ralpha). We have identified 14-3-3zeta as an hIL-9Ralpha-interacting protein. We also mapped residues 518-522 (Arg-Ser(519)-Trp-Thr(521)-Phe) in hIL-9Ralpha and helix I of 14-3-3zeta as being important for interaction. Moreover, peptide competition experi-ments suggested that interaction between hIL-9Ralpha and 14-3-3zeta requires the phosphorylation of Ser(519) or Thr(521). This is the first demonstration that 14-3-3 can interact with a non-tyrosine kinase receptor. The interaction between 14-3-3 and IL-9Ralpha but not IL-4Ralpha also suggests a potential role for 14-3-3 in determining cytokine specificity.
Sliva
D
D
Department of Medicine (Hematology/Oncology), Indiana University School of Medicine, and Indiana Cancer Research Institute, 1044 W. Walnut Street, R4-272, Indianapolis, IN 46202, USA.
Gu
M
M
Zhu
Y X
YX
Chen
J
J
Tsai
S
S
Du
X
X
Yang
Y C
YC
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001665:Binding Sites; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011233:Precipitin Tests; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000235:genetics / Q000378:metabolism*; D053651:Receptors, Interleukin-9; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; C508045:IL9R protein, human; D020033:Protein Isoforms; D011506:Proteins; D018123:Receptors, Interleukin; D053651:Receptors, Interleukin-9; D013912:Threonine; D012694:Serine; D014446:Tyrosine 3-Monooxygenase