title: Effect of certain chemicals on the reproduction of medaka (Oryzias latipes).
abstract: In order to understand the effects of estrogenic chemicals on fish reproduction, we exposed male medaka (Oryzias latipes) to a natural estrogen [17 beta-estradiol (17 beta-E2)] and three estrogenic chemicals [bisphenol-A, nonylphenol (NP) and di(2-ethylhexyl)phthalate (DEHP)]. After two weeks' exposure, one male medaka was kept together with two female medaka for spawning, and the number of eggs and hatchings were compared to those of a negative control group. The results indicated that exposure to 17 beta-E2 caused a significant decrease in the number of eggs and hatchings as compared to the negative control group at and above 3 nmol/l. Also, the highest concentrations of bisphenol-A and NP caused a decrease in the number of hatchings, but no decrease in hatchings was observed in DEHP treatments. In the treatment using these chemicals the decrease in egg numbers was not so much as in hatching numbers. When compared to other in vitro studies, concentrations observed to have adverse effects on reproduction in this study are generally lower. In addition, it was suggested that physical alterations, such as an induction of plasma vitellogenin, were caused at much lower concentrations than those at which a decline in reproductivity was actually induced.
authors.affiliation: Tokyo Metropolitan Research Institute for Environmental Protection 1-7-5, Japan. w_meiko@tokyo-eiken.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Shioda
authors.forename: M
authors.initials: M
authors.lastname: Wakabayashi
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D004051:Diethylhexyl Phthalate / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology*; D005260:Female; D008297:Male; D009990:Oryzias / Q000502:physiology*; D010063:Ovum / Q000187:drug effects; D010636:Phenols / Q000494:pharmacology; D012098:Reproduction / Q000187:drug effects*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004051:Diethylhexyl Phthalate; D004958:Estradiol; D004967:Estrogens; D004968:Estrogens, Non-Steroidal; D010636:Phenols; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds