The common cDNA and amino acid sequences of the CD14 (myeloid cell-specific leucine-rich glycoprotein) receptor.
The CD14 receptor is a myeloid cell specific receptor, which plays a role in the recognition of lipopolysaccharides (endotoxins of gram-negative bacteria) and cell stimulation. To date, several sequences of the cDNA of the CD14 receptor have been described. We sought to establish whether the substitutions C(230)-->G, and G(560)-->A are polymorphic or if they result from a PCR or sequencing error. Using two mismatched PCRs, we confirmed (on 75 unrelated probands) that the substitutions are not due to common polymorphisms. The common cDNA sequence has the C in position 230 and G in position 560. This corresponds to the amino acids Ala and Cys in positions 77 and 187, respectively.
Hubácek
J A
JA
Institute for Clinical and Experimental Medicine, Prague, Czech Republic. jaroslav.hubacek@medicon.cz
Poledne
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R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000737:chemistry* / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011817:Rabbits; D051381:Rats; D015183:Restriction Mapping
D018076:DNA, Complementary; D018950:Lipopolysaccharide Receptors