title: Neonatal screening for congenital toxoplasmosis in the Poznań region of Poland by analysis of Toxoplasma gondii-specific IgM antibodies eluted from filter paper blood spots.
abstract: OBJECTIVE
  The aims of the study were to determine the prevalence of congenital toxoplasmosis at birth in the Poznań region of Poland, the value of the serologic examination of filter paper blood specimens collected from newborns for the diagnosis of congenital Toxoplasma infection and the duration of anti-Toxoplasma-specific IgM antibodies in infants' sera.
  
  
  METHODS
  All neonates born in the maternity wards of the University Hospital of Gynaecology and Obstetrics in Poznań and in 10 selected obstetrics wards in the district hospitals were included. Blood samples were collected on filter paper cards, between the first and sixth day of life, screened for anti-Toxoplasma-specific IgM antibodies by an immunocapture enzyme-linked immunosorbent assay and if positive further analyzed for specific IgG and IgA antibodies.
  
  
  RESULTS
  Between June, 1996, and October, 1998, filter paper samples from 27,516 liveborn infants were tested, which constituted approximately 75% of all births and 83% of liveborn neonates from the Poznań region. Anti-T. gondii-specific IgM antibodies were found in 13 newborns, equivalent to a prevalence of Toxoplasma-specific IgM in newborns of 1 per 2,117 liveborn children (0.47 per 1,000) or 1 per 870 children (1.15 per 1,000) born to seronegative women at risk of primary T. gondii infection during pregnancy. We identified two congenitally infected infants who were IgM-negative at birth, had a classic triad of clinical symptoms during the first year of life and had high levels of specific IgG. The birth prevalence of congenital toxoplasmosis in the Poznań region was at least 1 per 1,834 live births (0.55 per 1,000) or 1 per 754 live neonates born to seronegative women (1.33 per 1,000). The sensitivity of the IgM assay on eluate from filter paper was not more than 86.7%, and the mean duration of IgM detectable by enzyme-linked immunosorbent assay in serum samples was the first 4.8 weeks of life.
  
  
  CONCLUSIONS
  In Poland the screening for congenital toxoplasmosis detecting one case per each 2,000 live births could be considered for inclusion in existing national neonatal screening programs for phenylketonuria and congenital hypothyroidism.
authors.affiliation: Department of Medical Microbiology, Institute of Microbiology and Infectious Diseases, Karol Marcinkowski University of Medical Sciences, Poznań, Poland. mpaul@eucalyptus.usoms.poznan.pl
authors.forename: M
authors.initials: M
authors.lastname: Paul
authors.forename: E
authors.initials: E
authors.lastname: Petersen
authors.forename: Z S
authors.initials: ZS
authors.lastname: Pawlowski
authors.forename: J
authors.initials: J
authors.lastname: Szczapa
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001800:Blood Specimen Collection / Q000379:methods; D016001:Confidence Intervals; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D015994:Incidence; D007231:Infant, Newborn; D008297:Male; D015997:Neonatal Screening / Q000379:methods*; D016012:Poisson Distribution; D011044:Poland / Q000453:epidemiology; D011247:Pregnancy; D014122:Toxoplasma / Q000276:immunology*; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology*; D014505:Urban Population
chemical_list: D000913:Antibodies, Protozoan; D007075:Immunoglobulin M