The endothelial monocyte-activating polypeptide II (EMAP II) is a substrate for caspase-7.
Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which the ASTD cleavage site was changed to the sequence ASTA, was not processed by caspase-7. The caspase-7-mediated generation and release of mature EMAP II may provide a mechanism for leukocyte recruitment to sites of programmed cell death, and thus may link apoptosis to inflammation.
Behrensdorf
H A
HA
Department of Molecular Cell Biology, Max-Planck-Institute for Physiological and Clinical Research, Parkstr. 1, 61231, Bad Nauheim, Germany.
van de Craen
M
M
Knies
U E
UE
Vandenabeele
P
P
Clauss
M
M
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines; D017931:DNA Primers / Q000235:genetics; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D016297:Mutagenesis, Site-Directed; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D009363:Neoplasm Proteins; D011498:Protein Precursors; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C077219:small inducible cytokine subfamily E, member 1; C505409:Casp3 protein, mouse; C505524:Casp7 protein, mouse; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases