[Loss of imprinting of insulin-like growth factor II gene in acute myeloid leukemia].
OBJECTIVE
To investigate the status of insulin- like growth factor-2(IGF-2) gene imprinting in acute myeloid leukemia(AML).


METHODS
Selection of heterozygous cases for Apa I polymorphism within exon 9 of IGF-2 gene and further analysis of loss of imprinting(LOI) were done in 26 AML patients by polymerase chain reaction(PCR), reverse transcription PCR (RT-PCR) and restriction fragment length polymorphism(RFLP) technique.


RESULTS
Eleven cases of AML patients were identified as heterozygous for IGF-2(11/26); 8 heterozygous patients showed biallelic expression of IGF-2, indicating loss of IGF-2 imprinting.


CONCLUSIONS
The frequent LOI of IGF-2 may play a role in the development of acute myeloid leukemia.
Liu
Y
Y
Institute of Clinical Medical Sciences, Affiliated Hospital, Wannan Medical College, Wuhu, Anhui, 241001 P.R. China. Chenwork@163.net
Yang
B
B
Zhu
H
H
Wang
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D018392:Genomic Imprinting; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged
D007335:Insulin-Like Growth Factor II