A method for bacteriocin quantification.
Different aspects of the most commonly used assay methods in the study of bacteriocins were examined. The conditions under which extraction and incubation (including exposure time) take place were analysed, and several different formal models that are usually employed to calculate ID50 were compared. As an alternative designed to overcome the problems which characterize the response of micro-organisms that are sensitive to bacteriocins, an operative procedure in a liquid medium and a modified re-parameterized logistic equation is proposed. When applied to the inhibition of Leuconostoc mesenteroides by nisin, the model allows an optimal experimental procedure to be defined.
Cabo
M L
ML
Instituto de Investigacións Mariñas, Vigo, Pontevedra, Spain. marta@iim.csic.es
Murado
M A
MA
González
M P
MP
Pastoriza
L
L
D000818:Animals; D001430:Bacteriocins / Q000302:isolation & purification* / Q000494:pharmacology; D003470:Culture Media; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D013294:Lactococcus lactis / Q000737:chemistry; D007934:Leuconostoc / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D012474:Salmon / Q000382:microbiology
D001430:Bacteriocins; D003470:Culture Media