Influence of osmotic and nutritional stress on physiology of Penicillium fellutanum in removal of phosphocholine and modification of phospho-1-O-[N-peptidyl-(2-aminoethanol)] phosphodiesters of peptidophosphogalactomannan species.
Addition of 3 M NaCl to 72-h cultures of Penicillium fellutanum in 2 mM phosphate resulted in an increase in percentage of extracellular peptidophosphogalactomannan III (pP(x)GM(iii)) and a decrease in that of pP(x)GM(ii). The magnitude of (31)P nuclear magnetic resonance signals at 1.47 and 1.33 ppm of phospho-1-O-[N-peptidyl-(2-aminoethanol)] phosphodiesters pP(x)GM(ii) and pP(x)GM(iii) decreased compared with controls. The data suggest that serine, glycine, and threonine residues from the 3-kDa peptide and from galactofuranosyl-6-O-phospho-1'-O-[N-peptidyl-(2-aminoethanol)] residues were the precursors of the needed choline-derived osmolytes.
Park
Y I
YI
Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611-0700, USA.
Bonetti
S J
SJ
Gander
J E
JE
D000596:Amino Acids / Q000032:analysis; D002240:Carbohydrate Sequence; D003470:Culture Media; D005690:Galactose / Q000031:analogs & derivatives; D006025:Glycosaminoglycans / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans; D008969:Molecular Sequence Data; D010755:Organophosphates / Q000737:chemistry / Q000378:metabolism*; D009997:Osmotic Pressure; D010407:Penicillium / Q000502:physiology*; D010761:Phosphorus Radioisotopes; D010767:Phosphorylcholine / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D006025:Glycosaminoglycans; D008351:Mannans; D010755:Organophosphates; D010761:Phosphorus Radioisotopes; D010767:Phosphorylcholine; C012990:galactomannan; D012965:Sodium Chloride; D005690:Galactose