IL-10 reduces grain dust-induced airway inflammation and airway hyperreactivity.
To determine whether interleukin-10 (IL-10) could alter the development of grain dust-induced airway disease, we pretreated mice with either saline or IL-10 intravenously, exposed the mice to an inhalation challenge with corn dust extract (CDE), and measured inflammation and the development of airway hyperreactivity. Pretreatment with IL-10, in comparison to saline, reduced the concentration and percentage of polymorphonuclear cells in the lavage fluid 30 min after the inhalation challenge with CDE (P < 0. 05). In comparison to saline-treated mice, IL-10 did not significantly alter the degree of airway hyperreactivity 30 min after the exposure to CDE. IL-10-treated mice lavaged 18 h after challenge with CDE also exhibited a lower percentage of polymorphonuclear cells in the lavage fluid (P < 0.05) and had significantly less airway hyperreactivity than did mice pretreated with the saline placebo (P < 0.05). These findings indicate that exogenous IL-10 is effective in reducing airway inflammation and airway hyperreactivity due to the inhalation of CDE.
Quinn
T J
TJ
Pulmonary, Critical Care, and Occupational Medicine Division, Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA.
Taylor
S
S
Wohlford-Lenane
C L
CL
Schwartz
D A
DA
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D016535:Bronchial Hyperreactivity / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D004391:Dust / Q000009:adverse effects*; D007249:Inflammation / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D016753:Interleukin-10 / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008173:Lung Diseases, Obstructive / Q000209:etiology / Q000276:immunology / Q000517:prevention & control; D008297:Male; D016210:Methacholine Chloride; D051379:Mice; D008810:Mice, Inbred C57BL; D015846:Monokines / Q000032:analysis / Q000235:genetics; D020556:Neutrophil Infiltration; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012140:Respiratory Tract Diseases / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D003313:Zea mays
D000893:Anti-Inflammatory Agents; D004391:Dust; D008070:Lipopolysaccharides; D015846:Monokines; D012333:RNA, Messenger; D016210:Methacholine Chloride; D016753:Interleukin-10