Characterization of alpha(1)-adrenoceptor subtypes in the eye.
The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and alpha(1B)-adrenoceptors were detected and the proportion of sites was 70% alpha(1A)- and 30% alpha(1B)-adrenoceptors, respectively. In the pig iris, ciliary body and choroid, which are melanin-rich tissues, the non-specific binding of [(3)H]-prazosin was too high to detect any of the alpha(1)-adrenoceptor subtypes. However, in the albino rabbit iris, ciliary body and retina both alpha(1A)- and alpha(1B)-adrenoceptors were detected. The proportion of sites in the iris was 60 % alpha(1A)- and 40% alpha(1B)-adrenoceptors, respectively. In the ciliary body and rabbit retina the proportion of sites were 70% alpha(1A)- and 30% alpha(1B)-adrenoceptors. Only the alpha(1A)-adrenoceptor subtype was detected in the rabbit choroid.
Wikberg-Matsson
A
A
Department of Ophthalmology, University Hospital, Uppsala, Sweden.
Uhlén
S
S
Wikberg
J E
JE
D000818:Animals; D002829:Choroid / Q000737:chemistry; D002924:Ciliary Body / Q000737:chemistry; D005123:Eye / Q000737:chemistry*; D007498:Iris / Q000737:chemistry; D011224:Prazosin; D011817:Rabbits; D011869:Radioligand Assay; D018340:Receptors, Adrenergic, alpha-1 / Q000032:analysis*; D012160:Retina / Q000737:chemistry; D013552:Swine
D018340:Receptors, Adrenergic, alpha-1; D011224:Prazosin