[Comparative study of the reactability of phosphoric acid residues in tRNA(Ser) and tRNA(Leu) from Thermus thermophilus].
The reactivity of phosphates in the Thermus thermophilus tRNA(Ser) (GCU) and tRNA(Leu) (CAG) was studied using the ethylnitrosourea modification. It was shown that phosphates of nucleotides 58-60 (T loop), 20-22 (D loop), and 48 (at the junction of the variable and T stems) were poorly modified in both tRNAs. The most pronounced differences in the reactivity were observed for phosphates at the junctions of the variable stem with T-stem (47q, 49) and anticodon stem (45). This indicates differences in orientations of the long variable arm relative to the backbone in the tRNAs studied.
Kovalenko
O P
OP
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Petrushenko
Z M
ZM
Kriklivyĭ
I A
IA
Iaremchuk
A D
AD
Tukalo
M A
MA
D000926:Anticodon; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010756:Phosphoric Acids / Q000737:chemistry*; D012356:RNA, Transfer, Leu / Q000737:chemistry* / Q000235:genetics; D012362:RNA, Transfer, Ser / Q000737:chemistry* / Q000235:genetics; D016963:Thermus thermophilus / Q000235:genetics*
D000926:Anticodon; D010756:Phosphoric Acids; D012356:RNA, Transfer, Leu; D012362:RNA, Transfer, Ser; C030242:phosphoric acid