Application of Combinatorial Procedures in the Search for Serine-Protease-Like Activity with Focus on the Acyl Transfer Step.
Recursive deconvolution of a 729-membered peptide library has identified three active sequences, in which both Ser and His are present in one of the two tripeptidic chains generated on a steroidal scaffold (see structural formula), for the cleavage of an activated p-nitrophenyl ester. This combinatorial approach aims at searching for serine-protease-like activity.
De Muynck H
Laboratory for Organic Synthesis Department of Organic Chemistry, University of Gent Krijgslaan 281, 9000 Gent (Belgium).
Madder
A
Farcy
N
De Clercq PJ
Pérez-Payán
MN
Öhberg
LM
Davis
AP