title: Properties of the Macrophomina phaseolina endoglucanase (EGL 1) gene product in bacterial and yeast expression systems.
abstract: Functional expression of a beta-D-1,4 glucanase-encoding gene (egl1) from a filamentous fungus was achieved in both Escherichia coli and Saccharomyces cerevisiae using a modified version of pRS413. Optimal activity of the E. coli-expressed enzyme was found at incubation temperatures of 60 degrees C, whereas the enzyme activity was optimal at 40 degrees C when expressed by S. cerevisiae. Enzyme activity at different pH levels was similar for both bacteria and yeast, being highest at 5.0. Yeast expression resulted in a highly glycosylated protein of approx 60 kDa, compared to bacterial expression, which resulted in a protein of 30 kDa. The hyperglycosylated protein had reduced enzyme activity, indicating that E. coli is a preferred vehicle for production scale-up.
authors.affiliation: University of California, Berkeley 94720, USA.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: R W
authors.initials: RW
authors.lastname: Jones
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D015153:Blotting, Western; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D002480:Cellulase; C090774:endoglucanase EGL1