title: Rare occurrence of classical Hodgkin's disease as a T cell lymphoma.
abstract: Recent work identified Hodgkin and Reed-Sternberg (H/RS) cells in classical Hodgkin's disease (cHD) as clonal progeny of mature B cells. Therefore, it is generally assumed that cHD homogenously represents a B cell lymphoma. In a subset of cHD, however, H/RS cells expressing T cell-associated proteins may be candidates for alternative lineage derivation. Single H/RS cells with cytotoxic T cell phenotype were micromanipulated from three cases of cHD and analyzed by single cell polymerase chain reaction for immunoglobulin heavy (IgH) and light chain (IgL) gene rearrangements, T cell receptor (TCR)-beta gene rearrangements, and germline configuration of the IgH and TCR-beta loci. H/RS cells from two cases of cHD harbored clonal, somatically mutated Ig gene rearrangements, whereas TCR-beta loci were in germline configuration. In contrast, H/RS cells from an additional case harbored clonal TCR-beta variable/diversity/joining (VDJ) and DJ gene rearrangements, whereas the IgH locus was in germline configuration on both alleles. Thus, in two cases of cHD with H/RS cells expressing cytotoxic T cell molecules, the tumor cells are derived from mature B cells that aberrantly express T cell markers. In a third case, however, H/RS cells were derived from a T cell, demonstrating that cHD can also occur as a T cell lymphoma.
authors.affiliation: Institute for Genetics, Department of Immunology, Universität zu Köln, Germany. markus.mueschen@uni-koeln.de
authors.forename: M
authors.initials: M
authors.lastname: Müschen
authors.forename: K
authors.initials: K
authors.lastname: Rajewsky
authors.forename: A
authors.initials: A
authors.lastname: Bräuninger
authors.forename: A S
authors.initials: AS
authors.lastname: Baur
authors.forename: J J
authors.initials: JJ
authors.lastname: Oudejans
authors.forename: A
authors.initials: A
authors.lastname: Roers
authors.forename: M L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: R
authors.initials: R
authors.lastname: Küppers
mesh_terms: D000328:Adult; D001483:Base Sequence; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D016399:Lymphoma, T-Cell / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016539:Reed-Sternberg Cells / Q000276:immunology*