title: Can hTNF-alpha be successfully produced and secreted in filamentous fungus Aspergillus niger?
abstract: A gene-fusion expression strategy was applied for the heterologous expression of hTNF-alpha in A. niger AB1.13. The TNF-alpha gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not present in the culture medium. From the expression of two hTNF-alpha analogues, LK 811 (Cys95/148) and LK 802 (Cys95/148, His107/108) respectively, we concluded that oligomerisation was not the critical point for secretion of hTNF-alpha in A. niger, but more probably improper folding already at the stage of monomer formation, or even incorrect processing of the molecule during the secretion pathway.
authors.affiliation: National Institute of Chemistry, Ljubljana, Slovenia.
authors.forename: N
authors.initials: N
authors.lastname: Krasevec
authors.forename: C A
authors.initials: CA
authors.lastname: van den Hondel
authors.forename: R
authors.initials: R
authors.lastname: Komel
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D019677:Artificial Gene Fusion; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics; D006801:Humans; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D005087:Glucan 1,4-alpha-Glucosidase