title: Kinetic analysis of a molecular model of the budding yeast cell cycle.
abstract: The molecular machinery of cell cycle control is known in more detail for budding yeast, Saccharomyces cerevisiae, than for any other eukaryotic organism. In recent years, many elegant experiments on budding yeast have dissected the roles of cyclin molecules (Cln1-3 and Clb1-6) in coordinating the events of DNA synthesis, bud emergence, spindle formation, nuclear division, and cell separation. These experimental clues suggest a mechanism for the principal molecular interactions controlling cyclin synthesis and degradation. Using standard techniques of biochemical kinetics, we convert the mechanism into a set of differential equations, which describe the time courses of three major classes of cyclin-dependent kinase activities. Model in hand, we examine the molecular events controlling "Start" (the commitment step to a new round of chromosome replication, bud formation, and mitosis) and "Finish" (the transition from metaphase to anaphase, when sister chromatids are pulled apart and the bud separates from the mother cell) in wild-type cells and 50 mutants. The model accounts for many details of the physiology, biochemistry, and genetics of cell cycle control in budding yeast.
authors.affiliation: Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg Virginia 24061, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Csikasz-Nagy
authors.forename: B
authors.initials: B
authors.lastname: Gyorffy
authors.forename: J
authors.initials: J
authors.lastname: Val
authors.forename: B
authors.initials: B
authors.lastname: Novak
authors.forename: J J
authors.initials: JJ
authors.lastname: Tyson
mesh_terms: D002453:Cell Cycle; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins / Q000235:genetics; D004271:DNA, Fungal / Q000096:biosynthesis; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D018628:Gene Dosage; D047908:Intracellular Signaling Peptides and Proteins; D000072235:Mating Factor; D008958:Models, Molecular; D009154:Mutation; D010455:Peptides / Q000378:metabolism; D010675:Pheromones / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013997:Time Factors
chemical_list: C082733:BCK2 protein, S cerevisiae; C093749:CLB2 protein, S cerevisiae; C082721:CLN2 protein, S cerevisiae; C079895:CLN3 protein, S cerevisiae; D019926:Cyclin B; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins; D004271:DNA, Fungal; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010455:Peptides; D010675:Pheromones; D010750:Phosphoproteins; C086926:SIC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000072235:Mating Factor