title: The partial substitution of digestible protein with gelatinized starch as an energy source reduces susceptibility to lipid oxidation in rainbow trout (Oncorhynchus mykiss) and sea bass (Dicentrarchus labrax) muscle.
abstract: We evaluated the influence of dietary gelatinized starch and protein on the fatty acid composition of muscle in rainbow trout and European sea bass and on the susceptibility of flesh to lipid peroxidation. The possibility that flesh peroxidation could be accounted for by lipogenesis and the deposition of fat was also explored. The inclusion of gelatinized starch in the diet of rainbow trout improved growth with respect to that observed in fish fed crude starch (P<.001). This was especially noticeable at the lowest concentration of dietary protein tested (P = .037); suggesting that gelatinized starch may partially replace protein in the production of energy without inducing a negative effect on growth. However, in European sea bass, the gelatinization of starch and dietary protein concentration showed no significant effect on final body weight. The intramuscular neutral lipid concentration of the sea bass was reduced by the gelatinization of dietary starch (P = .034). The highest dietary protein concentration increased the proportion of saturated fatty acids in the neutral (P = .0742) and polar (P = .0033) lipid fractions. The dietary inclusion of high levels of protein in rainbow trout led to a lower concentration of total (n-3) (P = .0457) and (n-6) (P = .0522) fatty acids and a higher concentration of total monounsaturated fatty acids (P = .0006). The inclusion of gelatinized starch led to a lower concentration of (n-3) fatty acids (P = .0034) and a higher concentration of saturated fatty acids (P = .0007). The polar fraction was hardly affected by the same treatment. A significantly lower susceptibility of the dorsal muscle to oxidation was observed in groups of European sea bass fed gelatinized starch (P<.01). A similar trend was observed in rainbow trout, although differences were not significant. The findings suggest that the digestible protein concentration of nutrient-dense diets for rainbow trout and European sea bass can be reduced with a beneficial effect on tissue lipid oxidation and no negative effects on growth and muscle composition.
authors.affiliation: Departamento de Bioquímica y Biología Molecular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Madrid, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Alvarez
authors.forename: C J
authors.initials: CJ
authors.lastname: López-Bote
authors.forename: A
authors.initials: A
authors.lastname: Diez
authors.forename: G
authors.initials: G
authors.lastname: Corraze
authors.forename: J
authors.initials: J
authors.lastname: Arzel
authors.forename: J
authors.initials: J
authors.lastname: Dias
authors.forename: S J
authors.initials: SJ
authors.lastname: Kaushik
authors.forename: J M
authors.initials: JM
authors.lastname: Bautista
mesh_terms: D000818:Animals; D001492:Bass / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion; D004734:Energy Metabolism; D005227:Fatty Acids / Q000032:analysis; D005780:Gelatin; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D009132:Muscles / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010084:Oxidation-Reduction; D013213:Starch
chemical_list: D004040:Dietary Carbohydrates; D004044:Dietary Proteins; D005227:Fatty Acids; D005780:Gelatin; D013213:Starch