title: Autoantibodies to DEK oncoprotein in human inflammatory disease.
abstract: OBJECTIVE
  To evaluate the specificity of anti-DEK antibodies for juvenile rheumatoid arthritis (JRA).
  
  
  METHODS
  Anti-DEK autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) using affinity-purified his6-DEK fusion protein. Sera from 639 subjects (417 patients with systemic autoimmune disease, 13 with sarcoidosis, 44 with pulmonary tuberculosis, 125 with uveitis, and 6 with scleritis, and 34 healthy control subjects) were screened. Reactivity was verified by immunoblotting and immunoprecipitation studies using baculovirus-expressed human DEK.
  
  
  RESULTS
  Anti-DEK activity was found at the following frequencies: JRA 39.4% (n = 71), systemic lupus erythematosus (SLE) 25.1% (n = 216), sarcoidosis 46.2% (n = 13), rheumatoid arthritis 15.5% (n = 71), systemic sclerosis 36.0% (n = 22), polymyositis 6.2% (n = 16), and adult Still's disease 0% (n = 21). Autoantibodies also were detected in 9.1% of tuberculosis sera (n = 44), but were undetectable in sera from the 34 healthy controls. Western blot and immunoprecipitation assay results correlated well with the ELISA findings. In general, levels of anti-DEK autoantibodies were higher in SLE than in other patient subsets, including JRA.
  
  
  CONCLUSIONS
  Anti-DEK autoantibodies are less specific for JRA than previously believed. They are produced in association with a variety of inflammatory conditions, many of which are associated with granuloma formation and/or predominant Thl cytokine production. Anti-DEK antibodies may be a marker for a subset of autoimmunity associated with interferon-gamma production rather than a particular disease subset.
authors.affiliation: Thurston Arthritis Research Center and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA.
authors.forename: X
authors.initials: X
authors.lastname: Dong
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: F N
authors.initials: FN
authors.lastname: Kabir
authors.forename: M
authors.initials: M
authors.lastname: Shaw
authors.forename: A M
authors.initials: AM
authors.lastname: Reed
authors.forename: L
authors.initials: L
authors.lastname: Stein
authors.forename: L E
authors.initials: LE
authors.lastname: Andrade
authors.forename: V F
authors.initials: VF
authors.lastname: Trevisani
authors.forename: M L
authors.initials: ML
authors.lastname: Miller
authors.forename: T
authors.initials: T
authors.lastname: Fujii
authors.forename: M
authors.initials: M
authors.lastname: Akizuki
authors.forename: L M
authors.initials: LM
authors.lastname: Pachman
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: W H
authors.initials: WH
authors.lastname: Reeves
mesh_terms: D000293:Adolescent; D000328:Adult; D001171:Arthritis, Juvenile / Q000208:ethnology / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology; D002648:Child; D002868:Chromosomal Proteins, Non-Histone; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000208:ethnology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D015513:Oncogene Proteins / Q000276:immunology*; D000075223:Poly-ADP-Ribose Binding Proteins; D011994:Recombinant Proteins / Q000276:immunology; D012507:Sarcoidosis / Q000208:ethnology / Q000276:immunology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D017678:Sex Distribution; D016706:Still's Disease, Adult-Onset / Q000208:ethnology / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000208:ethnology / Q000276:immunology; D014605:Uveitis / Q000453:epidemiology / Q000276:immunology
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D011994:Recombinant Proteins