Pharmacokinetics of radioiodinated growth hormones in the turtle Chrysemys dorbigni.
Growth hormone binding proteins (GHBP) have been identified in the blood of many species. The aim of the present work is to study the physiological role of the GHBP in the turtle serum which we recently described. Binding studies were carried out using in vivo pharmacokinetic and chromatographic techniques as well as in vitro methods. When (125)I-GH was injected in physiological concentration into Chrysemys dorbigni turtles, the first step of pharmacokinetics was the binding of a significant fraction of the labeled GH by the GHBPs present in serum. The decay curve followed a three compartments model and gave the equation: Ae(-alphat) + Be(-betat) + Ce(-gammat). The fast compartment with t(1/2) of 14.4 min or 25.2 min, for hGH and bGH represents 30.3% and 18.9% of total radioactivity, respectively, at hypothetical time zero (not experi mental). Chromatographic studies reveal that this rapid compartment represents free GH. The second and third compartments represent complex forms between GH and GHBPs present in the turtle serum, and represent 70% and 80% of total radioactivity for hGH and bGH, respectively. In vitro chromatographic studies showed direct evidence of the presence of GHBPs in the turtle serum. The presence of these GHBPs changed the pharmacokinetics of labeled GH in plasma and the subsequent liver uptake of GH. The labeled hGH or bGH binds to turtle serum in similar proportion, but maximal liver uptake of these hormones are completely different (L/B ratio of 9.2 +/- 0.6 (n = 5) for ( 125)I-hGH and 4.8 +/- 0.3 (n = 7) for (125)I-bGH). The reasons for these differences could be that human GH binds to lactogenic and somatotropic receptors and bovine GH binds only to somatotropic receptors.
Turyn
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Instituto de Quimica Biológica (UBA-CONICET), Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.
Da Silva
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RS
Marques
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D000818:Animals; D002352:Carrier Proteins / Q000097:blood*; D002417:Cattle; D002850:Chromatography, Gel; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000493:pharmacokinetics*; D019382:Human Growth Hormone / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans; D007457:Iodine Radioisotopes / Q000097:blood / Q000493:pharmacokinetics; D008099:Liver / Q000378:metabolism; D008657:Metabolic Clearance Rate; D011485:Protein Binding; D011986:Receptors, Somatotropin / Q000378:metabolism; D013045:Species Specificity; D014426:Turtles / Q000097:blood / Q000502:physiology*
D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D011986:Receptors, Somatotropin; D019382:Human Growth Hormone; D013006:Growth Hormone; C051815:somatotropin-binding protein