title: A positive selection vector combining tetracycline resistance that eliminates the need for bacterial plating.
abstract: We describe here the construction of a plasmid that combines positive selection with tetracycline resistance. The vector comprises a modified version of the gene encoding the cytosine-specific DNA methyltransferase MspI and a modified form of the pBR322 tetA(C) gene. The combination of these two genes facilitates the selection of recombinant plasmids in broth cultures, thereby eliminating the need for bacterial plating.
authors.affiliation: The Transgenomic Research Laboratory, University of Sheffield, Western Bank, S10 2TN, United Kingdom.
authors.forename: M M
authors.initials: MM
authors.lastname: Matin
authors.forename: D P
authors.initials: DP
authors.lastname: Hornby
mesh_terms: D001431:Bacteriological Techniques; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D012641:Selection, Genetic; D013753:Tetracycline Resistance