[Role of staphylococcus aureus hemolytic toxin-alpha in pathogenesis of infectious endocarditis: studies in vitro].
OBJECTIVE
To study effects of alpha-toxin (AT) Staphylococcus aureus on human platelets and endothelial cells.


METHODS
Concentrations of intracellular calcium in human platelets and endothelial cells were estimated by fluorescence, phosphoinositide metabolism in the endothelial cells was studied using 3H-myoinositole.


RESULTS
AT induced a dose-dependent increase of intracellular calcium in blood and vascular cells, stimulates dose-dependent formation of inositol phosphates in endothelial cells.


CONCLUSIONS
AT action on the platelets and endothelial cells results in a significant receptor-independent rise in concentration of intracellular calcium, activation of phosphoinositide metabolism, death of cells. These data support the hypothesis that the platelet and endothelial cell damage is mostly due to the passive Ca2+ influxvia pores formed by AT in cellular membrane.
Baliakina
E V
EV
Gerasimovskaia
E V
EV
Romanov
Iu A
IuA
Atakhanov
Sh E
ShE
D001427:Bacterial Toxins / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004697:Endocarditis, Bacterial / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006460:Hemolysin Proteins / Q000494:pharmacology*; D006801:Humans; D007295:Inositol Phosphates / Q000096:biosynthesis; D013203:Staphylococcal Infections / Q000097:blood / Q000382:microbiology* / Q000473:pathology; D013211:Staphylococcus aureus / Q000472:pathogenicity
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007295:Inositol Phosphates; C021091:staphylococcal alpha-toxin; D002118:Calcium