Non-quantal acetylcholine release is increased after nitric oxide synthase inhibition.
After anticholinesterase treatment, depolarization of the postsynaptic muscle membrane by about 5 mV develops due to non-quantally released acetylcholine from the motor nerve terminal and can be revealed as hyperpolarization by the addition of curare (H-effect). The H-effect increases significantly to 8.7 mV after inhibition of NO-synthase by L-nitroarginine methylester (L-NAME) whilst no changes in the amplitude and frequency of quantal miniature endplate potentials are observed.
Mukhtarov
M R
MR
Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Russia.
Vyskocil
F
F
Urazaev
A K
AK
Nikolsky
E E
EE
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D003964:Diaphragm / Q000294:innervation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D008564:Membrane Potentials / Q000187:drug effects; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009411:Nerve Endings / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000187:drug effects / Q000378:metabolism; D003473:Neuromuscular Nondepolarizing Agents / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D017981:Receptors, Neurotransmitter / Q000037:antagonists & inhibitors / Q000502:physiology; D014403:Tubocurarine / Q000494:pharmacology
D002800:Cholinesterase Inhibitors; D004791:Enzyme Inhibitors; D003473:Neuromuscular Nondepolarizing Agents; D017981:Receptors, Neurotransmitter; D019001:Nitric Oxide Synthase; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester; D014403:Tubocurarine