Elimination of the interferences by keto-opiates in the GC-MS analysis of 6-monoacetylmorphine.
A simple procedure to eliminate the interference from keto-opiates in the analysis of 6-monoacetylmorphine (6-MAM) by gas chromatography-mass spectrometry is described. The pretreatment of urine samples with sodium bisulfite followed by solid-phase extraction results in the elimination of the bisulfite addition products formed from the reaction of the bisulfite ions with the carbonyl carbon of the keto-opiates. This simple procedure results in the accurate quantitation of 6-MAM at a concentration of 4 ng/mL in the presence of keto-opiates up to 10,000 ng/mL.
Singh
J
J
Veterans Affairs Medical Center, Laboratory Service, Minneapolis, Minnesota 55417, USA.
Burke
R E
RE
Mertens
L E
LE
D000975:Antioxidants / Q000494:pharmacology; D003429:Cross Reactions; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D009022:Morphine Derivatives / Q000652:urine*; D009294:Narcotics / Q000737:chemistry*; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods; D013447:Sulfites / Q000494:pharmacology
D000975:Antioxidants; D009022:Morphine Derivatives; D009294:Narcotics; D013447:Sulfites; C026979:6-O-monoacetylmorphine; C009279:sodium bisulfite