title: Biomolecular-chemical screening: a novel screening approach for the discovery of biologically active secondary metabolites. II. Application studies with pure metabolites.
abstract: The novel screening strategy called "biomolecular-chemical screening" combines the advantages of the chemical screening approach--the analysis of the chromatographic and chemical behaviour of secondary metabolites on TLC plates--with binding studies of these molecules with bio-macromolecules like DNA. This approach was advantageously used to detect the interaction of pure compounds with DNA. In order to prove the reliability of the biomolecular-chemical screening and to examine DNA-binding properties, 470 pure secondary metabolites were analysed by this method. Besides the confirmation of already known binders with the TLC-based method, for a number of natural products DNA-binding properties were discovered for the first time. In consequence, binding of pure compounds can be measured by 1D TLC in a reliable and easy manner, in which DNA is applied together with the test compound at the starting spot. Analysis is performed via differences in Rf-values in comparison to a reference chromatogram without DNA.
authors.affiliation: Hans-Knöll-Institut für Naturstoff-Forschung e.V., Jena, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Maier
authors.forename: C
authors.initials: C
authors.lastname: Maul
authors.forename: M
authors.initials: M
authors.lastname: Zerlin
authors.forename: S
authors.initials: S
authors.lastname: Grabley
authors.forename: R
authors.initials: R
authors.lastname: Thiericke
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004247:DNA / Q000378:metabolism*
chemical_list: D004247:DNA