The mechanism of joint capsule thermal modification in an in-vitro sheep model.
The purpose of this study was to understand the mechanism responsible for joint capsule shrinkage after nonablative laser application in an in-vitro sheep model. Femoropatellar joint capsular tissue specimens harvested from 20 adult sheep were treated with one of three power settings of a holmium:yttrium-aluminum-garnet laser or served as a control. Laser treatment significantly shortened the tissue and decreased tissue stiffness in all three laser groups, whereas failure strength was not altered significantly by laser treatment. Transmission electron microscopic examination showed swollen collagen fibrils and loss of membrane integrity of fibroblasts. A thermometric study revealed nonablative laser energy caused tissue temperature to rise in the range of 64 degrees C to 100 degrees C. Electrophoresis after trypsin digestion of the tissue revealed significant loss of distinct alpha bands of Type I collagen in laser treated samples, whereas alpha bands were present in laser treated tissue without trypsin digestion. The results of this study support the concept that the primary mechanism responsible for the effect of nonablative laser energy is thermal denaturation of collagen in joint capsular tissue associated with unwinding of the triple helical structure of the collagen molecule.
Hayashi
K
K
Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, Univeristy of Wisconsin, Madison 53706, USA.
Peters
D M
DM
Thabit
G
G
Hecht
P
P
Vanderby
R
R
Fanton
G S
GS
Markel
M D
MD
D000704:Analysis of Variance; D000818:Animals; D001494:Baths / Q000379:methods; D001696:Biomechanical Phenomena; D005260:Female; D005269:Femur / Q000378:metabolism / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure; D006358:Hot Temperature; D066298:In Vitro Techniques; D017746:Joint Capsule / Q000378:metabolism / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure; D053685:Laser Therapy; D016018:Least-Squares Analysis; D008854:Microscopy, Electron; D010329:Patella / Q000378:metabolism / Q000503:physiopathology* / Q000601:surgery / Q000648:ultrastructure; D011897:Random Allocation; D012756:Sheep; D013696:Temperature; D013821:Thermometers