title: Surface plasmon resonance (SPR) analysis of coagulation in whole blood with application in prothrombin time assay.
abstract: It is previously shown that surface plasmon resonance (SPR) can be used to study blood plasma coagulation. This work explores the use of this technique for the analysis of tissue factor induced coagulation, i.e. prothrombin time (PT) analysis, of whole blood and plasma. The reference method was nephelometry. The prothrombin time analysis by SPR was performed by mixing two volumes of blood/plasma, one volume of thromboplastin, and one volume of CaCl2 solution directly on a sensor surface. The measurements show good agreement between nephelometry and SPR plasma analysis and also between SPR plasma and whole blood analysis. The effect of anticoagulant treatment on the clotting times was significant both quantitatively and qualitatively. The impact on the SPR signal of different physiological events in the coagulation process is discussed, and tentative interpretations of the sensorgram features are given. The major advantage of the SPR method compared to nephelometry is the possibility to perform analysis on whole blood instead of plasma. In conclusion, SPR is a promising method for whole blood coagulation analysis.
authors.affiliation: Department of Biomedicine and Surgery, University Hospital, Linköping, Sweden. kenha@ifm.liu.se
authors.forename: K M
authors.initials: KM
authors.lastname: Hansson
authors.forename: T P
authors.initials: TP
authors.lastname: Vikinge
authors.forename: M
authors.initials: M
authors.lastname: Rånby
authors.forename: P
authors.initials: P
authors.lastname: Tengvall
authors.forename: I
authors.initials: I
authors.lastname: Lundström
authors.forename: K
authors.initials: K
authors.lastname: Johansen
authors.forename: T L
authors.initials: TL
authors.lastname: Lindahl
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D005069:Evaluation Studies as Topic; D006801:Humans; D066298:In Vitro Techniques; D009391:Nephelometry and Turbidimetry; D010949:Plasma; D011517:Prothrombin Time; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000925:Anticoagulants