title: Application of the cell block method, utilizing mount quick mounting medium.
abstract: Our objective was to determine the applicability of cell transfer and cell block methods using Mount Quick (Daido Sangyo, Saitama, Japan) mounting medium (MQ) for hematoxylin-eosin (H&E) and immunohistochemical staining of several limited amounts of biological materials in slide preparations. The materials investigated were histopathologically confirmed malignant mesotheliomas (pleural effusions) and malignant lymphomas, a malignant melanoma, and an amelanotic melanoma in sealed slides. Monoclonal antibodies against carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), cancer antigen 125 (CA-125), vimentin, thrombomodulin (TM), cytokeratin, UCHL-1, L-26, melanoma-specific antigen (HMB45), and S-100 protein (S-100) were applied in the investigation. The malignant mesotheliomas were found to be positive for EMA, cytokeratin, vimentin, TM, and CA-125, and negative for CEA, with no differences being observed in findings from direct contact preparations. Using T-cell-type malignant lymphomas for immunohistochemistry, UCHL-1 positivity and L-26 negativity were clearly demonstrated. The malignant melanoma and amelanotic melanoma materials stained strongly for HMB45 and S-100. Cell transfer employing MQ is a suitable approach for immunohistochemical investigations of limited materials. In addition, cell blocks derived from MQ-embedded smears can be used for both H&E and immunohistochemical staining. Diagn. Cytopathol. 2000;22:117-119.
authors.affiliation: Department of Cytopathology, Fujita Health University School of Health Sciences, Toyoake, Aichi, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Kaneko
authors.forename: M
authors.initials: M
authors.lastname: Funahashi
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: T K
authors.initials: TK
authors.lastname: Kobayashi
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D008223:Lymphoma / Q000175:diagnosis / Q000378:metabolism; D008297:Male; D018328:Melanoma, Amelanotic / Q000175:diagnosis / Q000378:metabolism; D008654:Mesothelioma / Q000175:diagnosis / Q000378:metabolism; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D010996:Pleural Effusion / Q000175:diagnosis / Q000378:metabolism; D010997:Pleural Neoplasms / Q000175:diagnosis / Q000378:metabolism; D012878:Skin Neoplasms / Q000175:diagnosis / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor