title: Protective effect of erythropoietin on the oxidative damage of erythrocyte membrane by hydroxyl radical.
abstract: Treatment of red blood cells with the copper (II) ascorbate system causes increased lipid peroxidation, increased membrane microviscosity, and phospholipid translocation with a concurrent decrease in cytosolic catalase and glutathione peroxidase activities. All these changes are prevented if the cells are treated with erythropoietin prior to the exposure to copper (II) ascorbate. The present investigation further indicates that the oxidative damage brought about by copper (II) ascorbate is due to generation of hydroxyl radical and that erythropoietin plays a unique role in protecting the membrane from oxidative damage.
authors.affiliation: Indian Institute of Chemical Biology, Calcutta.
authors.forename: A
authors.initials: A
authors.lastname: Chattopadhyay
authors.forename: T D
authors.initials: TD
authors.lastname: Choudhury
authors.forename: D
authors.initials: D
authors.lastname: Bandyopadhyay
authors.forename: A G
authors.initials: AG
authors.lastname: Datta
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D004921:Erythropoietin / Q000494:pharmacology*; D006041:Goats; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D020011:Protective Agents / Q000494:pharmacology; D011994:Recombinant Proteins
chemical_list: D020011:Protective Agents; D011994:Recombinant Proteins; D004921:Erythropoietin; D017665:Hydroxyl Radical; D003300:Copper; D001205:Ascorbic Acid