Cancer immunomodulation by carbohydrate ligands for the lymphocyte receptor NKR-P1.
Natural killer (NK) and T cells express a superfamily of proteins with structural features of C-type lectins. Recombinantly prepared soluble form of rat NKR-P1 (CD161) recognized carbohydrate GalNac and GlcNac moieties. Ganglioside GM2 and heparin related-IS oligosaccharides representing the high affinity ligands for this receptor, increased the sensitivity of targets for killing by the rat effectors isolated from blood and spleen in vitro. Based on these results, we investigated in vivo the possible therapeutic effect of GM2 and IS carried by liposomes during induced rat colorectal carcinogenesis. The reduction of cancer incidence versus the controls (50% vs 88.88%), approached the 5-fluorouracil treatment (41.66%).
Pospisil
M
M
Department of Immunology and Gnotobiology, Laboratory of Natural Cell Immunity, Institute of Microbiology, Academy of Sciences, Prague 14220, Czech Republic.
Vannucci
L
L
Horvath
O
O
Fiserova
A
A
Krausova
K
K
Bezouska
K
K
Mosca
F
F
D000818:Animals; D000954:Antigens, Surface / Q000187:drug effects; D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use; D001397:Azoxymethane; D002273:Carcinogens; D015179:Colorectal Neoplasms / Q000139:chemically induced / Q000188:drug therapy; D004337:Drug Carriers; D004354:Drug Screening Assays, Antitumor; D004926:Escherichia coli; D005472:Fluorouracil / Q000627:therapeutic use; D005678:G(M2) Ganglioside / Q000627:therapeutic use*; D037181:Lectins, C-Type; D008081:Liposomes; D008297:Male; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009844:Oligosaccharides / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000954:Antigens, Surface; D000964:Antimetabolites, Antineoplastic; D002273:Carcinogens; D004337:Drug Carriers; D037181:Lectins, C-Type; D008081:Liposomes; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009844:Oligosaccharides; D005678:G(M2) Ganglioside; D001397:Azoxymethane; D005472:Fluorouracil