title: Structure and regulation of the dnaA promoter region in three Streptomyces species.
abstract: The regulatory region of the Streptomyces dnaA gene comprises a single promoter and two DnaA boxes that are located upstream of the promoter. Comparative analysis of the dnaA promoter region from S. chrysomallus, S. lividans and S. reticuli revealed that the location, spacing and orientation of the DnaA boxes are conserved. In vitro studies demonstrated that efficient binding of the Streptomyces DnaA protein to DNA requires the presence of two DnaA boxes. In vivo analysis of dnaA promoter mutants deleted for one or both DnaA boxes indicated that the dnaA gene is autoregulated. However, the degree of derepression observed is relatively modest.
authors.affiliation: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
authors.forename: D
authors.initials: D
authors.lastname: Jakimowicz
authors.forename: J
authors.initials: J
authors.lastname: Majka
authors.forename: B
authors.initials: B
authors.lastname: Lis
authors.forename: G
authors.initials: G
authors.lastname: Konopa
authors.forename: G
authors.initials: G
authors.lastname: Wegrzyn
authors.forename: W
authors.initials: W
authors.lastname: Messer
authors.forename: H
authors.initials: H
authors.lastname: Schrempf
authors.forename: J
authors.initials: J
authors.lastname: Zakrzewska-Czerwinska
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria