Aligned microcontact printing of micrometer-scale poly-L-lysine structures for controlled growth of cultured neurons on planar microelectrode arrays.
We describe a method for producing high-resolution chemical patterns on surfaces to control the attachment and growth of cultured neurons. Microcontact printing has been extended to allow the printing of micron-scale protein lines aligned to an underlying pattern of planar microelectrodes. Poly-L-lysine (PL) lines have been printed on the electrode array for electrical studies on cultured neural networks. Rat hippocampal neurons showed a high degree of attachment selectivity to the PL and produced neurites that faithfully grew onto the electrode recording sites.
James
C D
CD
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
Davis
R
R
Meyer
M
M
Turner
A
A
Turner
S
S
Withers
G
G
Kam
L
L
Banker
G
G
Craighead
H
H
Isaacson
M
M
Turner
J
J
Shain
W
W
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002455:Cell Division; D006624:Hippocampus / Q000166:cytology; D008839:Microelectrodes; D009431:Neural Conduction; D009474:Neurons / Q000166:cytology*; D011107:Polylysine; D051381:Rats; D013499:Surface Properties
D011107:Polylysine