title: Temperature-sensitive strain of Cryptococcus neoformans producing hyphal elements in a feline nasal granuloma.
abstract: We report the isolation of a temperature-sensitive, serotype A, mating type alpha strain of Cryptococcus neoformans from a case of nasal cryptococcosis in a cat. The strain grew extremely slowly at 35 degrees C and failed to grow at 37 degrees C in vitro. Histopathological sections of the infected tissue revealed yeast cells producing hyphae up to several hundred micrometers in length, in addition to numerous encapsulated yeast cells typical of C. neoformans. The cultures grown on yeast extract-peptone-glucose agar at 35 degrees C also produced some yeast cells with germ tube-like hyphal elements up to 100 microm in length.
authors.affiliation: Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, Knoxville, Tennessee 37996, USA. bemis@utk.edu
authors.forename: D A
authors.initials: DA
authors.lastname: Bemis
authors.forename: D J
authors.initials: DJ
authors.lastname: Krahwinkel
authors.forename: L A
authors.initials: LA
authors.lastname: Bowman
authors.forename: P
authors.initials: P
authors.lastname: Mondon
authors.forename: K J
authors.initials: KJ
authors.lastname: Kwon-Chung
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D003453:Cryptococcosis / Q000382:microbiology / Q000662:veterinary*; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000302:isolation & purification*; D005260:Female; D006099:Granuloma / Q000382:microbiology / Q000662:veterinary*; D009296:Nasal Cavity / Q000473:pathology*; D013696:Temperature