Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria.
The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
Heeb
S
S
Laboratoire de Biologie Microbienne, Université de Lausanne, Switzerland.
Itoh
Y
Y
Nishijyo
T
T
Schnider
U
U
Keel
C
C
Wade
J
J
Walsh
U
U
O'Gara
F
F
Haas
D
D
D000595:Amino Acid Sequence; D001483:Base Sequence; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010944:Plants / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D011551:Pseudomonas fluorescens / Q000235:genetics*; D012093:Replicon / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D004274:DNA, Recombinant