Native plasmids of Fusobacterium nucleatum: characterization and use in development of genetic systems.
Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.
Haake
S K
SK
Divisions of Associated Clinical Sciences, UCLA School of Dentistry, Los Angeles, California 90095-1668, USA. shaake@dent.ucla.edu
Yoder
S C
SC
Attarian
G
G
Podkaminer
K
K
D000595:Amino Acid Sequence; D004254:DNA Nucleotidyltransferases; D004269:DNA, Bacterial / Q000032:analysis; D004706:Endodeoxyribonucleases / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D016967:Fusobacterium nucleatum / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; C110340:DNA relaxase; D004706:Endodeoxyribonucleases