Molecular characterization of the diversity of Campylobacter spp. isolates collected from a poultry slaughterhouse: analysis of cross-contamination.
Investigations of a free-range broiler flock during the rearing period and at the slaughterhouse by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the flagellin (flaA) gene (flaA typing) have shown that poultry carcasses are contaminated by Campylobacter spp. strains which were previously present in the poultry faces. Moreover, the investigation of the previous and the following batches in the processing plant using flaA typing have shown that cross-contamination between batches coming from different flocks occurs and is also a risk factor for the presence of Campylobacter spp. on poultry carcasses.
Rivoal
K
K
Agence Française de Sécurité, Sanitaire des Aliments (AFSSA), Ploufragan, France.
Denis
M
M
Salvat
G
G
Colin
P
P
Ermel
G
G
D000003:Abattoirs; D000818:Animals; D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005408:Flagellin / Q000235:genetics; D005511:Food Handling; D014644:Genetic Variation; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012867:Skin / Q000382:microbiology
D005408:Flagellin; C067138:flaA protein, bacteria