The discriminatory power of ribo-PCR compared to conventional ribotyping for epidemiological purposes.
Molecular typing techniques have become increasingly important for confirmation of epidemiological relationships and delimitation of nosocomial outbreaks. The discriminatory power of the two DNA-based typing methods, conventional ribotyping and ribo-PCR, was assessed to distinguish between selected strains of Acinetobacter calcoaceticus, Enterobacter cloacae, Serratia marcescens and Pseudomonas aeruginosa. Overall, conventional ribotyping was more discriminatory than ribo-PCR.
Severino
P
P
Molecular Biology Laboratory, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
Darini
A L
AL
Magalhães
V D
VD
D016954:Acinetobacter calcoaceticus / Q000145:classification / Q000235:genetics; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D016972:Enterobacter cloacae / Q000145:classification / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics; D012706:Serratia marcescens / Q000145:classification / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial