Development of a plasma 17alpha-hydroxyprogesterone time resolved-fluorescence immunoassay involving a new biotinylated tracer.
A biotinylated 17alpha-hydroxyprogesterone probe (3) was prepared from 17alpha-hydroxyprogesterone-3-carboxymethyloxime and conjugate obtained by acylation of biotinylaminopropylammonium trifluroacetate. This new tracer was used in the development of a 17alpha-hydroxyprogesterone time-resolved fluoroimmunoassay using streptavidin-europium. The new method was compared to a long-standing radioimmunoassay method and found to be more sensitive and economical.
Boudi
A
A
Laboratoire de Chimie Organique Faculté de Pharmacie, Paris, France.
Giton
F
F
Galons
H
H
Eulry
B
B
Villette
J M
JM
Soliman
H
H
Boudou
P
P
Fiet
J
J
D019326:17-alpha-Hydroxyprogesterone / Q000097:blood*; D000312:Adrenal Hyperplasia, Congenital / Q000378:metabolism; D001667:Binding, Competitive; D019921:Biotinylation; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards; D006801:Humans; D008297:Male; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000378:metabolism; D017697:Premenopause; D011863:Radioimmunoassay; D012015:Reference Standards; D012680:Sensitivity and Specificity; D014316:Tritium
D015335:Molecular Probes; D014316:Tritium; D019326:17-alpha-Hydroxyprogesterone