Induction of stress proteins in response to hypoxia.
Hypoxia is a severe stress factor to which man and most other mammalian species are capable of adapting. However, the cellular mechanism which enable cells to adapt are still unknown. Effect of hypoxia was studied on the synthesis of hypoxia induced proteins in rat kidney and in vero cell line (monkey kidney). These were exposed to hypoxia at 240 mmHg pressure for 1 hr. The induction of stress protein was determined by probing with monoclonal antibodies against 65 kDa heat shock protein (hsp65). The induction of a 65 kDa protein was 3.6 fold higher to the total cellular protein, both in cell lines and kidney of rats. In vivo response was predominantly observed in renal cortical region particularly in glomeruli. The induction of stress proteins during hypoxia suggests their importance in the maintenance of cellular integrity under hypoxia.
Ganju
L
L
Defence Institute of Physiology & Allied Sciences, Delhi, India.
Chanda
S
S
Karan
D
D
Srivastava
K K
KK
D000818:Animals; D001426:Bacterial Proteins; D015687:Cell Hypoxia / Q000502:physiology; D018834:Chaperonin 60; D018833:Chaperonins / Q000096:biosynthesis; D002522:Chlorocebus aethiops; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D000860:Hypoxia / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014709:Vero Cells
D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins