A constitutively open potassium channel formed by KCNQ1 and KCNE3.
Mutations in all four known KCNQ potassium channel alpha-subunit genes lead to human diseases. KCNQ1 (KvLQT1) interacts with the beta-subunit KCNE1 (IsK, minK) to form the slow, depolarization-activated potassium current I(Ks) that is affected in some forms of cardiac arrhythmia. Here we show that the novel beta-subunit KCNE3 markedly changes KCNQ1 properties to yield currents that are nearly instantaneous and depend linearly on voltage. It also suppresses the currents of KCNQ4 and HERG potassium channels. In the intestine, KCNQ1 and KCNE3 messenger RNAs colocalized in crypt cells. This localization and the pharmacology, voltage-dependence and stimulation by cyclic AMP of KCNQ1/KCNE3 currents indicate that these proteins may assemble to form the potassium channel that is important for cyclic AMP-stimulated intestinal chloride secretion and that is involved in secretory diarrhoea and cystic fibrosis.
Schroeder
B C
BC
Zentrum für Molekulare Neurobiologie Hamburg, Hamburg University, Germany.
Waldegger
S
S
Fehr
S
S
Bleich
M
M
Warth
R
R
Greger
R
R
Jentsch
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003106:Colon / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004563:Electrochemistry; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism; D051656:KCNQ Potassium Channels; D051657:KCNQ1 Potassium Channel; D051379:Mice; D008969:Molecular Sequence Data; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D051381:Rats; D014981:Xenopus
D051656:KCNQ Potassium Channels; D051657:KCNQ1 Potassium Channel; C496909:KCNQ1 protein, human; C496910:Kcnq1 protein, mouse; C496911:Kcnq1 protein, rat; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; C059983:potassium channel protein I(sk); D000242:Cyclic AMP