Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and btk.
Using immunoglobulin heavy chain transgenic mice, we show that B cell clones reaching the long-lived pool are heterogeneous: some are enriched in the CD21(high) compartment (mostly marginal zone [MZ]), others reside primarily in the follicles (FO). Altering the composition of the B cell receptor through N region additions decreases the rate of clonal production and the MZ enrichment. This process can be recapitulated by purified CD21(low) B cells and is due to a preferential clonal survival that requires a functional btk tyrosine kinase. We also show that generation of the MZ population is dependent on CD19. These findings suggest that the MZ B cell repertoire is positively selected and have functional implications for antigenic responses effected by B cells from this microenvironment.
Martin
F
F
Department of Microbiology, University of Alabama at Birmingham, 35294-3300, USA.
Kearney
J F
JF
D000077329:Agammaglobulinaemia Tyrosine Kinase; D000818:Animals; D018941:Antigens, CD19 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002999:Clone Cells; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D007130:Immunoglobulin Idiotypes; D016130:Immunophenotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D011505:Protein-Tyrosine Kinases / Q000378:metabolism / Q000502:physiology*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D017464:Receptors, Complement 3d / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D013154:Spleen / Q000166:cytology
D018941:Antigens, CD19; D007130:Immunoglobulin Idiotypes; D011947:Receptors, Antigen, B-Cell; D017464:Receptors, Complement 3d; D017455:Receptors, IgE; D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase