Apoptosis is a prominent feature of acute anterior uveitis in the Fischer 344 rat.
OBJECTIVE
To examine the hypothesis that apoptosis of infiltrating cells contributes to spontaneous resolution of uveitis in clinically relevant rodent models.


METHODS
Experimental melanin induced uveitis (EMIU) was induced in Fischer 344 rats by immunisation with 250 microg bovine ocular melanin. Endotoxin induced uveitis (EIU) was induced by injection of 200 microg Escherichia coli lipopolysaccharide. Formalin fixed, paraffin embedded ocular cross sections were stained by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labelling (TUNEL) to identify apoptotic cells. Indirect immunoperoxidase staining of paraformaldehyde lysine periodate fixed tissue cross sections was used to demonstrate expression of inducible nitric oxide synthase (iNOS).


RESULTS
TUNEL positive mononuclear cells were observed in the anterior uvea during both EMIU and EIU at all selected time points. However, whereas the majority of mononuclear cells appeared apoptotic from the outset of disease, neutrophils were notably TUNEL negative at all time points examined. Many infiltrating neutrophils expressed iNOS.


CONCLUSIONS
Apoptosis occurs early in the course of rat EMIU and EIU, and may contribute to resolution of these diseases. In general, infiltrating mononuclear cells die rapidly, while neutrophils survive, producing inducible nitric oxide synthase which may contribute to disease pathogenesis.
Smith
J R
JR
Department of Ophthalmology, Flinders University of South Australia, Adelaide, Australia.
Hart
P H
PH
Standfield
S D
SD
Coster
D J
DJ
Wing
S J
SJ
Williams
K A
KA
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D005128:Eye Diseases / Q000503:physiopathology; D005260:Female; D020287:In Situ Nick-End Labeling; D008297:Male; D009504:Neutrophils / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D051381:Rats; D011916:Rats, Inbred F344; D014606:Uveitis, Anterior / Q000201:enzymology / Q000503:physiopathology*
D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat