title: Nucleus reuniens thalami innervates gamma aminobutyric acid positive cells in hippocampal field CA1 of the rat.
abstract: The aim of the present study was to investigate whether the nucleus reuniens thalami (RE) innervates inhibitory cells in hippocampal field CA1. Therefore, we examined the RE-CA1 input at the ultrastructural level. RE axons were anterogradely labeled with biotin-conjugated dextran amine (BDA), in combination with pre-embedding gamma aminobutyric acid (GABA)-immunolabeling of neurons in CA1. We observed that part of the BDA-labeled axons formed asymmetrical (i.e. excitatory) synapses on GABA-positive dendrites. Based on these data, which are in line with our previously published electrophysiological observations (Dolleman-Van der Weel, M.J., Lopes da Silva, F.H. and Witter, M.P., Nucleus reuniens thalami modulates activity in hippocampal field CA1 through excitatory and inhibitory mechanisms. J. Neurosci., 17 (1997) 5640-5650), we propose that RE-CA1 input partially influences hippocampal transmission through activation of local inhibitory interneurons.
authors.affiliation: Graduate School for Neurosciences Amsterdam, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Dolleman-Van der Weel
authors.forename: M P
authors.initials: MP
authors.lastname: Witter
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives; D003712:Dendrites / Q000378:metabolism / Q000502:physiology; D003911:Dextrans; D005260:Female; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009433:Neural Inhibition / Q000502:physiology; D009474:Neurons / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology; D013787:Thalamic Nuclei / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes; C076397:biotinylated dextran amine; D005680:gamma-Aminobutyric Acid; D001710:Biotin