The isolation and sequence of canine interleukin-8 receptor.
A cDNA corresponding to canine IL-8 receptor has been cloned and sequenced. The cDNA was synthesized using RT-PCR, with oligonucleotide primers designed from conserved regions of published IL-8 receptors. The 5'-end was cloned by 5'-RACE and the 3'-end was cloned by 3'-RACE. The cDNA encodes a predicted full length IL-8r protein of 356 amino acids. At the nucleic acid level, the canine cDNA shows 83.9%, 82.4%/78.8%, 81.5%/78%, 81.4%/77.7%, 77.8% and 77.3%/71.9% identity to published sequences of bovine, human, gorilla, rabbit, mouse and rat IL8RB/IL8RA, respectively. The derived protein from the cDNA sequences shows 75.3%/70.3%, 75.3%/70.1%, 74.8%/69.4%, 70%/59%, and 69.7% identity to that of human, rabbit, gorilla, rat and mouse IL8RB/IL8RA homolog.
Chang
Y F
YF
Department of Population Medicine and Diagnostic Science, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. yc42@cornell.edu
Novosel
V
V
Chang
C F
CF
D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004285:Dogs; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D051381:Rats; D018123:Receptors, Interleukin / Q000235:genetics* / Q000378:metabolism; D023062:Receptors, Interleukin-8A; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D020121:5' Untranslated Regions; D015703:Antigens, CD; D017931:DNA Primers; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A