title: Developmental expression of the SH3BGR gene, mapping to the Down syndrome heart critical region.
abstract: The SH3BGR gene has been recently isolated and mapped to chromosome 21 within the Down syndrome (DS) congenital heart disease (CHD) minimal region. As a first step to evaluate the possible involvement of SH3BGR in CHD that affect 40% of DS patients, we have analyzed by in situ hybridization the expression pattern of the mouse homolog gene (Sh3bgr), during development. Our results show that Sh3bgr is already expressed at embryonic day 7.75 (E7.75) in the precardiogenic mesoderm and that from E8.5 to E10.5 its expression is restricted to the heart. In subsequent developmental stages, Sh3bgr transcripts are also detected in skeletal muscle and in some visceral smooth muscles including urinary bladder and gut wall, but not in vascular smooth muscle. Our results, demonstrating that Sh3bgr is expressed in earliest stages of mouse heart development, support a possible role of this gene in heart morphogenesis and, consequently, in the pathogenesis of CHD in DS.
authors.affiliation: Dipartimento di Scienze Biomediche, Centro CNR di Biologia e Fisiopatologia Muscolare, University of Padua, Padua, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Egeo
authors.forename: R
authors.initials: R
authors.lastname: Di Lisi1
authors.forename: C
authors.initials: C
authors.lastname: Sandri
authors.forename: M
authors.initials: M
authors.lastname: Mazzocco
authors.forename: M
authors.initials: M
authors.lastname: Lapide
authors.forename: S
authors.initials: S
authors.lastname: Schiaffino
authors.forename: P
authors.initials: P
authors.lastname: Scartezzini
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D004314:Down Syndrome / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D017403:In Situ Hybridization; D051379:Mice; D009124:Muscle Proteins / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; C104671:SH3BGR protein, human