title: Control of muscle cell-type specification in the zebrafish embryo by Hedgehog signalling.
abstract: The specification of different muscle cell types in the zebrafish embryo requires signals that emanate from the axial mesoderm. In previous studies we and others have shown that overexpression of different members of the Hedgehog protein family can induce the differentiation of two types of slow-twitch muscles, the superficially located slow-twitch fibres and the medially located muscle pioneer cells. Here we have investigated the requirement for Hedgehog signalling in the specification of these distinct muscle cell types in two ways: first, by characterising the effects on target gene expression and muscle cell differentiation of the u-type mutants, members of a phenotypic group previously implicated in Hedgehog signalling, and second, by analysing the effects of overexpression of the Patched1 protein, a negative regulator of Hedgehog signalling. Our results support the idea that most u-type genes are required for Hedgehog signalling and indicate that while such signalling is essential for slow myocyte differentiation, the loss of activity of one signal, Sonic hedgehog, can be partially compensated for by other Hedgehog family proteins.
authors.affiliation: Krebs Institute, University of Sheffield, United Kingdom.
authors.forename: K E
authors.initials: KE
authors.lastname: Lewis
authors.forename: P D
authors.initials: PD
authors.lastname: Currie
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: H
authors.initials: H
authors.lastname: Schauerte
authors.forename: P
authors.initials: P
authors.lastname: Haffter
authors.forename: P W
authors.initials: PW
authors.lastname: Ingham
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D017403:In Situ Hybridization; D051741:Kruppel-Like Transcription Factors; D008565:Membrane Proteins / Q000235:genetics; D009132:Muscles / Q000196:embryology* / Q000378:metabolism; D009154:Mutation; D017570:MyoD Protein / Q000235:genetics; D009218:Myosins / Q000378:metabolism; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface; D015398:Signal Transduction; D019170:Somites / Q000378:metabolism; D015534:Trans-Activators; D014157:Transcription Factors / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins; D000075746:Zinc Finger Protein Gli2
chemical_list: D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D008565:Membrane Proteins; D017570:MyoD Protein; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D015534:Trans-Activators; D014157:Transcription Factors; D029961:Zebrafish Proteins; D000075746:Zinc Finger Protein Gli2; C491715:ptch1 protein, zebrafish; D009218:Myosins