title: Retinoic acid modulates RAR alpha and RAR beta receptors in human glioma cell lines.
abstract: To identify retinoic acid (RA) signalling pathways involved in growth and differentiation in cells of the glial lineage, two human glioma ceh lines were studied. The three RA receptors (RARs) mRNAs were constitutively expressed, and of the three RXRs, RXR beta appeared predominant. Western blotting analysis confirmed the constitutive expression of RAR alpha and RAR beta. Treatment with all-trans-RA induced morphological changes in the two cell lines, which progressed from their normal pattern of randomly oriented spindle-shaped cells to fibroblast-like glial cells. RA up-regulated RAR alpha and RAR beta mRNAs in both cell lines. Interestingly, RA treatment up-regulated RAR beta proteins but not RAR alpha proteins, suggesting post-transcriptional regulations of RAR transcripts in glioma cells.
authors.affiliation: Laboratoire de Biologie Cellulaire Hématopoiétique, Institut d'Hématologie, Hôpital Saint-Louis, Paris, France. acarpent@club-internet.fr
authors.forename: A F
authors.initials: AF
authors.lastname: Carpentier
authors.forename: N
authors.initials: N
authors.lastname: Leonard
authors.forename: J
authors.initials: J
authors.lastname: Lacombe
authors.forename: F
authors.initials: F
authors.lastname: Zassadowski
authors.forename: R A
authors.initials: RA
authors.lastname: Padua
authors.forename: L
authors.initials: L
authors.lastname: Degos
authors.forename: C
authors.initials: C
authors.lastname: Daumas-Duport
authors.forename: C
authors.initials: C
authors.lastname: Chomienne
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D000072482:Retinoic Acid Receptor alpha; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: C000607071:RARA protein, human; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; C082925:retinoic acid receptor beta; D014212:Tretinoin