Cellulose acetate as solid phase in ELISA for plague.
Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter) which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 microg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 1:4,000. No relevant antigen lixiviation from the cellulose acetate was observed even after washing the discs 15 times. The discs were impregnated by the coloured products from the ELISA development allowing its use in dot-ELISA. Furthermore, cellulose acetate showed a better performance than the conventional PVC plates.
Barbosa
A D
AD
Laboratório de Imunopatologia Keizo Asami and Departamento de Química Fundamental, Universidade Federal de Pernambuco, Recife, PE, 50670-420, Brasil.
Barros
F S
FS
Callou
E Q
EQ
Almeida
A M
AM
Araujo
A M
AM
Azevedo
W M
WM
Carvalho
L B
LB
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002482:Cellulose; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D010930:Plague / Q000276:immunology*; D017186:Titrimetry; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D002482:Cellulose