title: Effects of methanol and temperature on enzyme immunoassay with monoclonal antibodies specific to the insecticide etofenprox.
abstract: We examined the effects of methanol and temperature on the reactivity of monoclonal antibodies specific to the insecticide etofenprox. When the antigen-antibody reaction was done at 4 degrees C in 10% methanol, the sensitivity in the enzyme immunoassay with each antibody was more than 10-fold higher than that measured at 37 degrees C. Although in 10% methanol one of the antibodies reacted equally with both etofenprox and the carbonate-derivative of etofenprox, in 50% methanol the antibody reacted with etofenprox, but not with the derivative.
authors.affiliation: Division of Natural Science, Osaka Kyoiku University, Japan. katagiri@cc.osaka-kyoiku.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Katagiri
authors.forename: T
authors.initials: T
authors.lastname: Kadoya
authors.forename: K
authors.initials: K
authors.lastname: Miyake
authors.forename: F
authors.initials: F
authors.lastname: Ishibashi
authors.forename: H
authors.initials: H
authors.lastname: Ohkawa
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007306:Insecticides / Q000032:analysis* / Q000276:immunology; D000432:Methanol; D011722:Pyrethrins / Q000032:analysis* / Q000276:immunology; D013696:Temperature; D013816:Thermodynamics
chemical_list: D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D007306:Insecticides; D011722:Pyrethrins; C076840:ethofenprox; D000432:Methanol