African swine fever virus EP153R open reading frame encodes a glycoprotein involved in the hemadsorption of infected cells.
The open reading frame EP153R, located within the EcoRI E' fragment of the African swine fever (ASF) virus genome, is predicted to encode a membrane protein of 153 amino acids that presents significant homology to the N-terminal region of several CD44 molecules. EP153R contains multiple putative sites for N-glycosylation, phosphorylation, and myristoylation, a central transmembrane region, a C-type animal lectin-like domain, and a cell attachment sequence. Transcription of EP153R takes place at both early and late times during the virus infection. The disruption of the gene, achieved by insertion of the marker gene LacZ within EP153R, did not change either the in vitro virus growth rate or the virus-sensitive/resistant condition of up to 17 established cell lines, but abrogated the hemadsorption phenomenon induced in ASF virus-infected cells. As the sequence and expression of the ASF virus protein pEP402R, a CD2 homolog responsible for the adhesion of erythrocytes to susceptible cells, was unaffected in cultures infected with the EP153R deletion mutant, we conclude that the gene EP153R is needed to induce and/or maintain the interaction between the viral CD2 homolog and its corresponding cell receptor.
Galindo
I
I
Centro de Biología Molecular "Severo Ochoa", Universidad Autónoma de Madrid, Madrid, 28049, Spain.
Almazán
F
F
Bustos
M J
MJ
Viñuela
E
E
Carrascosa
A L
AL
D000358:African Swine Fever Virus / Q000235:genetics* / Q000472:pathogenicity*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D017353:Gene Deletion; D005814:Genes, Viral; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics; D037102:Lectins / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics* / Q000502:physiology*
D004279:DNA, Viral; D018960:Hyaluronan Receptors; D037102:Lectins; D014764:Viral Proteins