title: An in vitro chemosensitivity test for colorectal cancer using collagen-gel droplet embedded cultures.
abstract: This study evaluated in vitro assay for chemosensitivity test using a collagen-gel droplet embedded culture drug sensitivity test (CD-DST) for colorectal cancer. CD-DST was performed in 24 patients with Dukes B, C colorectal cancer. Primary cultures of tumor cell samples from 87.5% (21/24) patients with colorectal cancer were successful. The efficacy rates assessed by CD-DST of five anticancer drugs evaluated were: 60.4% for adriamycin, 58.9% for etoposide, 56.7% for cisplatin, 53.4% for 5-fluorouracil, for 31.1% for mitomycin C, and 9.5% for vindesine. The present study demonstrated the clinical usefulness of CD-DST in evaluating the response to chemotherapy in colorectal cancer.
authors.affiliation: Department of Surgery, Kurume University Medical Center, Japan. kmc1@mocha.ocn.ne.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Araki
authors.forename: H
authors.initials: H
authors.lastname: Isomoto
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Kaibara
authors.forename: M
authors.initials: M
authors.lastname: Yasunaga
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Yatsugi
authors.forename: K
authors.initials: K
authors.lastname: Yamauchi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D003094:Collagen; D015179:Colorectal Neoplasms / Q000473:pathology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D003094:Collagen