Age-related changes in the modulatory action of gastrin-releasing peptide, neuropeptide Y and sulfated cholecystokinin octapeptide in the proliferation of murine lymphocytes.
Several investigations have suggested that the interactions between the nervous and immune systems are modified with age. The aim of the present work was to study the effect in vitro of three neuropeptides: gastrin-releasing peptide (GRP), neuropeptide Y (NPY) and sulfated cholecystokinin octapeptide (CCK-8s) on the spontaneous, as well as on the response to mitogen (concanavalin A), proliferative activity of spleen, thymus and axillary node leukocytes from adult (24 +/- 2 weeks), mature (50 +/- 2 weeks) and old (72 +/- 2 weeks) BALB/c male mice. In control samples, in the absence of neuropeptide, we observed a decreased lymphoproliferation in mature and old mice with respect to the adults in response to mitogen in the three organs studied. As regards, the effect of the neuropeptides, they stimulate the spontaneous proliferation of leukocytes from all locations, in adult animals, an effect that is decreased with ageing (in both mature and old animals). The proliferation in response to mitogen was significantly decreased by the neuropeptides in adults, this effect being progressively reduced with age.
Medina
S
S
Departamento de Biología Animal II (Fisiología Animal), Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, Madrid, Spain.
Rio
M D
MD
Cuadra
B D
BD
Guayerbas
N
N
Fuente
M D
MD
D000375:Aging / Q000276:immunology*; D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D019886:Gastrin-Releasing Peptide / Q000494:pharmacology*; D008198:Lymph Nodes / Q000254:growth & development / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009478:Neuropeptide Y / Q000494:pharmacology*; D018697:Nootropic Agents / Q000494:pharmacology; D012844:Sincalide / Q000031:analogs & derivatives* / Q000494:pharmacology; D013154:Spleen / Q000254:growth & development / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000254:growth & development / Q000276:immunology
C090019:8-sulfocholecystokinin octapeptide; D009478:Neuropeptide Y; D018697:Nootropic Agents; D003208:Concanavalin A; D019886:Gastrin-Releasing Peptide; D012844:Sincalide