Detection of ornithine decarboxylase mRNA in human breast cancer MCF-7 cells by in situ RT-PCR.
We have studied the expression of ornithine decarboxylase (ODC) mRNA by in situ hybridization and in situ RT-PCR in human breast cancer MCF-7 cell line. In situ RT-PCR demonstrated the overexpression of ODC mRNA, localized over the cytoplasm, while a very low signal was detected by in situ hybridization. Our findings indicate that in situ RT-PCR could represent a useful tool to study different levels of ODC expression in normal and tumor tissues. Since ODC expression is regulated by c-Myc oncoprotein, this model could be useful to monitor in vivo the effects of new anti-neoplastic molecules, specific inhibitors of c-Myc.
Clerico
L
L
Laboratory of Experimental Oncology, National Institute for Cancer Research, I-16132 Genova, Italy.
Mancuso
T
T
Crovella
S
S
Amoroso
A
A
Morgutti
M
M
Demori
E
E
Parodi
S
S
D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D009363:Neoplasm Proteins / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase