title: Internalization kinetics of the gonadotropin-releasing hormone (GnRH) receptor.
abstract: This study quantified the agonist-induced endocytotic and recycling events of the mammalian gonadotropin releasing hormone receptor (GnRH-R) and investigated the role of the intracellular carboxyl (C)-terminal tail in regulating agonist-induced receptor internalization kinetics. The rate of internalization for the rat GnRH-R was found to be exceptionally low when compared with G-protein coupled receptors (GPCRs) which possess a cytoplasmic C-terminal tail (thyrotropin-releasing hormone receptor (TRH-R), catfish GnRH-R (cfGnRH-R) and GnRH/TRH-R chimeric receptor). These data provide evidence that the presence of a functional intracellular cytoplasmic C-terminal tail is essential for rapid internalization of the studied GPCRs.
authors.affiliation: MRC Reproductive Biology Unit, Edinburgh, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Vrecl
authors.forename: A
authors.initials: A
authors.lastname: Heding
authors.forename: A
authors.initials: A
authors.lastname: Hanyaloglu
authors.forename: P L
authors.initials: PL
authors.lastname: Taylor
authors.forename: K A
authors.initials: KA
authors.lastname: Eidne
mesh_terms: D000818:Animals; D002397:Catfishes; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D051381:Rats; D011966:Receptors, LHRH / Q000819:agonists / Q000378:metabolism*
chemical_list: D011966:Receptors, LHRH