A new member of the GTPase superfamily that is upregulated in highly metastatic cells.
Two sublines of B16 melanoma cells, F10 and BL6, are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. We found a new member of the GTPase superfamily, namely TIB929, which displayed an induction of expression in BL6 cells. It conserved three consensus sequences for GTP-binding site motifs and showed a significant homology to the yeast Gtr2 gene throughout the coding sequence. TIB929 was expressed ubiquitously in human tumor cells, with a marked expression in highly metastatic cells. TIB929 was mapped on mouse chromosome 4D, syntenic to human chromosome 1p. The results suggested an involvement of TIB929 in malignant progression.
Nakaji
T
T
Second Department of Pathology, Kobe University Medical School, Japan.
Kataoka
T R
TR
Watabe
K
K
Nishiyama
K
K
Nojima
H
H
Shimada
Y
Y
Sato
F
F
Matsushima
H
H
Endo
Y
Y
Kuroda
Y
Y
Kitamura
Y
Y
Ito
A
A
Maeda
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D008546:Melanoma, Experimental / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D005810:Multigene Family; D009363:Neoplasm Proteins; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D014018:Tissue Distribution; D015854:Up-Regulation
D009363:Neoplasm Proteins; D012333:RNA, Messenger; C430722:RRAGC protein, human; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins