title: C-fos gene expression in rat brain around birth: effect of asphyxia and catecholamines.
abstract: At birth, the mammalian nervous system must adapt rapidly to the new conditions it encounters in the extra-uterine environment. One aspect of this adaptation, known as arousal, is mediated by catecholamines, the levels of which in the brain increase rapidly after birth. The pattern of gene expression also changes. Shortly after birth, expression of the immediate early gene c-fos, known to reflect general neural activity, is up-regulated. Furthermore, asphyxia often occurs in connection with birth. In order to examine the effects of this phenomenon on the expression of c-fos, as well as on the rate of noradrenaline (NA) turnover, asphyxia was induced in rat pups delivered by caesarean section. Northern blot analysis and in situ hybridization revealed that the increase in expression of c-fos in certain areas of the brain was greatly enhanced by asphyxia of moderate duration; whereas more prolonged asphyxia lowered the level of c-fos mRNA. Asphyxia had a similar effect on the rate of NA turnover. Adrenergic receptor antagonists administered prior to birth attenuated the birth-related induction of c-fos mRNA. However, the potentiation of c-fos expression by asphyxia was not altered by these antagonists. Therefore, we propose that while catecholamines play an important role in the induction of c-fos in the brain at birth, the effects of asphyxia involve a different mechanism.
authors.affiliation: Neonatal Program, Karolinska Institute, Astrid Lindgren's Children's Hospital, Stockholm, Sweden.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Tang
authors.forename: T
authors.initials: T
authors.lastname: Ringstedt
authors.forename: J
authors.initials: J
authors.lastname: Pequignot
authors.forename: H
authors.initials: H
authors.lastname: Lagercrantz
mesh_terms: D018674:Adrenergic Antagonists / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001238:Asphyxia Neonatorum / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology*; D016762:Genes, fos; D006801:Humans; D007231:Infant, Newborn; D009638:Norepinephrine / Q000502:physiology*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D018674:Adrenergic Antagonists; D012333:RNA, Messenger; D009638:Norepinephrine