Mechanoluminescence: lymphocyte analysis after exposure to ionizing radiation.
Lymphocyte mechanoluminescence (ML) develops as a result of mechanochemical activation of cells. This paper describes devices (tribogenerators) and techniques for lymphocyte mechanoluminescence analysis. At different stages of the cellular cycle the ML of non-irradiated peripheral blood lymphocytes (PBL) showed characteristic differences. After (60)Co gamma -irradiation of PBL in vitro at 0.25 Gy and 1 Gy an alteration of ML was observed with a relationship between ML values of lymphocytes, and also of mitochondria (MT), and the dose of ionizing radiation used. By using bioluminescence techniques it was shown that gamma-irradiation reduces the amount of ATP synthesized by PBL in the stationary phase.
Orel
V E
VE
Physics-Technical Laboratory, Ukrainian Research Institute of Oncology and Radiology, Lomonosova St. 33/43, 252022 Kyiv-22, Ukraine. orel@ucr.kiev.ua
Kadyuk
I N
IN
Dzyatkovskaya
N N
NN
Danko
M I
MI
Mel'nic
Y I
YI
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D049449:Luminescence; D008297:Male; D008928:Mitochondria / Q000528:radiation effects*; D051381:Rats
D003037:Cobalt Radioisotopes