title: High-level expression of canine parvovirus VP2 using Bombyx mori nucleopolyhedrovirus vector.
abstract: For the potential use as recombinant vaccine, canine parvovirus (CPV) major capsid protein VP2 was expressed using Bombyx mori nucleopolyhedrovirus (BmNPV) vector. CPV VP2 gene was introduced into polyhedrin-based BmNPV transfer vector pBmKSK3, and recombinant virus BmK1-Parvo was prepared. When anti-CPV.VP2 monoclonal antibody was employed in immunofluorescence staining, an intense signal was observed within BmK1-Parvo-infected Bm5 cells but not within uninfected cells or cells infected with a wild-type BmNPV-K1. In hemagglutination assay, the expression level of VP2 were 3.2 x 10(3) HA units/ml from infected Bm5 cells, 2.1x 10(5) HA units/larvae from infected larval fat body, and 1.6x 10(6) HA units/ml from infected larval hemolymph. These results suggested that BmNPV vector system using B. mori larva as host could be applied to efficient mass-production of recombinant vaccines.
authors.affiliation: Division of Applied Biology and Chemistry, Seoul National University, Suwon, Korea.
authors.forename: J Y
authors.initials: JY
authors.lastname: Choi
authors.forename: S D
authors.initials: SD
authors.lastname: Woo
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
authors.forename: H K
authors.initials: HK
authors.lastname: Hong
authors.forename: Y H
authors.initials: YH
authors.lastname: Je
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: J Y
authors.initials: JY
authors.lastname: Song
authors.forename: S H
authors.initials: SH
authors.lastname: An
authors.forename: S K
authors.initials: SK
authors.lastname: Kang
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics*; D036022:Capsid Proteins; D002478:Cells, Cultured; D004274:DNA, Recombinant / Q000235:genetics; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D005822:Genetic Vectors; D006386:Hemagglutination Tests; D006458:Hemolymph / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D000077183:Occlusion Body Matrix Proteins; D017993:Parvovirus, Canine / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins
chemical_list: D036022:Capsid Proteins; D004274:DNA, Recombinant; D000077183:Occlusion Body Matrix Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus