TGF-beta activates genes identified by differential mRNA display in pancreatic rudiments.
The effect of TGF-beta on gene activation in embryonic pancreatic rudiments was investigated using differential mRNA display. Several cDNA bands were augmented and some were suppressed in the presence of TGF-beta. Differentially expressed cDNAs were re-amplified, sequenced, and sequences compared to the GeneBank database. Glucagone and brain alpha-tropomyosin cDNAs were identified from the group of augmented cDNAs, and B-carboxypeptidase form the group of suppressed cDNAs. PCR experiments were confirmed with Northern blots. Obtained results are in accordance with immunohistochemical findings and render differential mRNA display a useful technique in identifying differentially expressed genes in embryonic pancreatic rudiments. Several unknown differentially expressed cDNA sequences obtained in our experiments remain to be identified.
Battelino
T
T
University Children's Hospital, Ljubljana, Slovenia.
Miralles
F
F
Krzisnik
C
C
Scharfmann
R
R
Czernichow
P
P
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D003626:Data Display; D004622:Embryo, Mammalian / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D010179:Pancreas / Q000196:embryology*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D015533:Transcriptional Activation; D016212:Transforming Growth Factor beta / Q000502:physiology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta