Differential subcellular distribution of neurolysin (EC 3.4.24.16) and thimet oligopeptidase (EC 3.4.24.15) in the rat brain.
Immunohistochemistry was used to analyze the rat brain distribution of thimet oligopeptidase and neurolysin. Both enzymes appear ubiquitously distributed within the entire rat brain. However, neuronal perikarya and processes stained for neurolysin, while intense nuclear labeling was only observed for thimet oligopeptidase. These data suggest that neurolysin and thimet oligopeptidase, endopeptidases sharing several functional and structural similarities, are present in distinctive intracellular compartments in neuronal cells.
Massarelli
E E
EE
Department of Histology, Biomedical Sciences Institute, USP, SP, Brazil.
Casatti
C A
CA
Kato
A
A
Camargo
A C
AC
Bauer
J A
JA
Glucksman
M J
MJ
Roberts
J L
JL
Hirose
S
S
Ferro
E S
ES
D000818:Animals; D001923:Brain Chemistry; D008297:Male; D008666:Metalloendopeptidases / Q000032:analysis*; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D008666:Metalloendopeptidases; C022510:thimet oligopeptidase; C062316:neurolysin