title: Secretion and dual regulation between epidermal growth factor and transforming growth factor-beta1 in MDA-MB-231 cell line in 42-hour-long cultures.
abstract: MDA-MB-231 is a breast cancer cell line which possesses large quantities of epidermal growth factor (EGF) receptors and specific high-affinity transforming growth factor-beta1 (TGF-beta1) receptors. We have established that these cells secrete constitutively measurable levels of EGF and TGF-beta1 in conditioned medium. The constitutive secretion of EGF decreased over time in culture (42 h), while the constitutive secretion of TGF-beta1 remained constant. TGF-beta1 secretion in EGF-treated cells was lower than in controls (P < 0.0001), but EGF concentrations were not modified after TGF-beta1 supplement. We postulate that in MDA-MB-231 cell line there is a dual regulation between both growth factors.
authors.affiliation: Biochemistry Department, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain.
authors.forename: P A
authors.initials: PA
authors.lastname: Martínez-Carpio
authors.forename: C
authors.initials: C
authors.lastname: Mur
authors.forename: M E
authors.initials: ME
authors.lastname: Fernández-Montolí
authors.forename: J M
authors.initials: JM
authors.lastname: Ramon
authors.forename: P
authors.initials: P
authors.lastname: Rosel
authors.forename: M A
authors.initials: MA
authors.lastname: Navarro
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D017077:Culture Media, Conditioned / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005260:Female; D006801:Humans; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D017077:Culture Media, Conditioned; D016212:Transforming Growth Factor beta; D004815:Epidermal Growth Factor