title: Growth characteristics of fibroblasts isolated from the trunk and distal aspect of the limb of horses and ponies.
abstract: OBJECTIVE
  To determine if there is a difference in in vitro growth of fibroblasts isolated from the trunk and distal aspect of the limb of horses and ponies. To determine the effects of a corticosteroid and monokine on in vitro growth of fibroblasts isolated from the trunk and distal aspect of the limb of horses and ponies.
  
  
  METHODS
  Growth of fibroblasts from tissues harvested from the trunk and limb were compared from horse and pony samples grown in control media and control media with triamcinolone or monokine added.
  
  
  METHODS
  Dermal and subcutaneous tissue from 22 horses and 17 ponies of various ages and breeds.
  
  
  METHODS
  Fibroblast growth was assessed by tritiated thymidine uptake using standard cell culture techniques. The effect of a monokine or triamcinolone plus control media were compared with control media for fibroblast growth.
  
  
  RESULTS
  Fibroblast growth from tissues isolated from the horse limb was significantly less than growth from the horse trunk and the limb and trunk of ponies. Monokine was more effective than triamcinolone in suppressing fibroblast growth from tissues isolated from the trunk and limb in both horses and ponies.
  
  
  CONCLUSIONS
  There are growth differences in fibroblasts isolated from the limb of horses compared with those isolated from the trunk and from the limb and trunk of ponies.
  
  
  CONCLUSIONS
  The difference in fibroblast growth from tissues isolated from the trunk and limb of horses and ponies may provide evidence for the difference reported in the healing characteristics of limb wounds in horses and ponies. Influencing fibroblast growth may provide a key to controlling the development of exuberant granulation tissue in horses and ponies.
authors.affiliation: Department of Veterinary Medicine and Surgery, University of Missouri College of Veterinary Medicine, Columbia 65211, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Miller
authors.forename: D A
authors.initials: DA
authors.lastname: Wilson
authors.forename: K G
authors.initials: KG
authors.lastname: Keegan
authors.forename: J M
authors.initials: JM
authors.lastname: Kreeger
authors.forename: E H
authors.initials: EH
authors.lastname: Adelstein
authors.forename: V K
authors.initials: VK
authors.lastname: Ganjam
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D005552:Forelimb; D006736:Horses / Q000502:physiology* / Q000601:surgery; D015846:Monokines / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*; D014221:Triamcinolone / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: D000893:Anti-Inflammatory Agents; D003470:Culture Media; D015846:Monokines; D014221:Triamcinolone