Characterization of potential regulatory elements within the rat arginine vasopressin proximal promoter.
In order to identify potential cis acting regulatory elements in the rat arginine vasopressin promoter, spanning nucleotides -374 to -36, we have carried out DNAase1 footprint analysis complemented by electrophoretic mobility shift analysis. This analysis has identified distinct transcription factor binding sites within this region. These are: 1) five E box motifs which are bound by members of the basic helix loop helix family of transcription factors; 2) two dGC rich elements which bind multiple complexes including Sp1; 3) a single AP1 element which binds a complex containing c-Fos or Fos related antigens. The location of these elements and their relationship to one another suggests combinatorial interaction amongst these factors as a mechanism of transcriptional regulation of the rat arginine vasopressin proximal promoter.
Grace
C O
CO
MRC Brain Metabolism Unit, Department of Pharmacology, University of Edinburgh, Edinburgh, UK.
Fink
G
G
Quinn
J P
JP
D000818:Animals; D001127:Arginine Vasopressin / Q000235:genetics*; D001483:Base Sequence; D018983:DNA Footprinting; D020862:GC Rich Sequence / Q000235:genetics; D005786:Gene Expression Regulation; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D051381:Rats; D018808:Transcription Factor AP-1 / Q000235:genetics
D015345:Oligonucleotide Probes; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1; D001127:Arginine Vasopressin