Steroid RU 486 inducible myogenesis by 10T1/2 fibroblastic mouse cells.
For reconstruction or repair of damaged tissues, an artificially regulated switch from proliferation to differentiation would be of great advantage. To achieve conditional myogenesis, we expressed MyoD in mouse C3H 10T1/2 fibroblastic cells, using a gene regulation system based on the synthetic steroid RU 486. By stable co-transfection of a plasmid construct with the RU 486 dependent activator and an integrating inducible MyoD construct, a cell clone, designated 10T-RM, was obtained in which MyoD expression was stringently controlled by RU 486. 12 h after addition of 10 nM RU 486 to 10T-RM cells, saturation levels of MyoD mRNA were observed and >/=2 days later, mRNA for embryonal myosin heavy chain (MyHC(emb)) was abundant and mononucleated cells fused into myotubes.
Bartsch
J W
JW
Developmental Biology and Molecular Pathology, University of Bielefeld, D-33501, Bielefeld, Germany.
Jäckel
M
M
Perz
A
A
Jockusch
H
H
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D015735:Mifepristone / Q000494:pharmacology*; D024510:Muscle Development; D009132:Muscles / Q000166:cytology* / Q000187:drug effects* / Q000196:embryology; D017570:MyoD Protein / Q000235:genetics; D018995:Myosin Heavy Chains / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects; D014162:Transfection
D017570:MyoD Protein; D012333:RNA, Messenger; D015735:Mifepristone; D018995:Myosin Heavy Chains