Irradiation of male rats increases the chromosomal sensitivity of progeny to genotoxic agents.
Chromosomal sensitivity to genotoxic agents was studied in the first generation progeny of male rats irradiated at a dose of 4.5 Gy X-rays and in the progeny of non-exposed animals. The frequency of anaphase chromosome aberrations (bridges or/and fragments) in rats exposed to X-rays or treated with cyclophosphamide was estimated: in proliferating hepatocytes (2 Gy) as a function of time during liver regeneration after partial hepatectomy; in bone marrow cells (2.5 Gy or 25 mg/kg body wt); in fetal fibroblasts (3 Gy). The sensitivity of chromosomes to genotoxic agents was found to be increased in the progeny of irradiated male rats as compared with the progeny of non-exposed animals. This finding provides supportive evidence that irradiation of parents is an important factor in predisposition of progeny to chromosomal instability.
Vorobtsova
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Laboratory of Radiation Genetics, Central Research Institute of Radiology and Roentgenology, Pesochny-2, Leningradskaya 70/4, 189646 St Petersburg, Russia. radgen@gate.la.spb.ru
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003520:Cyclophosphamide / Q000633:toxicity*; D005220:Fathers; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D018812:Paternal Exposure / Q000009:adverse effects*; D011247:Pregnancy; D051381:Rats
D009153:Mutagens; D003520:Cyclophosphamide