title: Levels of alpha- and beta-secretase cleaved amyloid precursor protein in the cerebrospinal fluid of Alzheimer's disease patients.
abstract: Alternative cleavage of the amyloid precursor protein (APP) results in generation and secretion of both soluble APP (sAPP) and beta-amyloid (Abeta). Abeta is the main component of the amyloid depositions in the brains of Alzheimer's disease (AD) patients. Using Western blotting, we compared the levels of alpha-secretase cleaved sAPP, beta-secretase cleaved sAPP and total sAPP, in cerebrospinal fluid (CSF) from 13 sporadic AD patients and 13 healthy controls. Our findings show significant amounts of beta-secretase cleaved sAPP in CSF. There was no statistically significant difference in the levels of beta-secretase cleaved sAPP between AD patients and controls. The levels of alpha-secretase cleaved sAPP and total sAPP were, however, found to be significantly lower in the AD patients than in the controls.
authors.affiliation: Division of Geriatric Medicine, Karolinska Institute, NEUROTEC, KFC NOVUM, Huddinge, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Sennvik
authors.forename: J
authors.initials: J
authors.lastname: Fastbom
authors.forename: M
authors.initials: M
authors.lastname: Blomberg
authors.forename: L O
authors.initials: LO
authors.lastname: Wahlund
authors.forename: B
authors.initials: B
authors.lastname: Winblad
authors.forename: E
authors.initials: E
authors.lastname: Benedikz
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D053829:Amyloid Precursor Protein Secretases; D016564:Amyloid beta-Protein Precursor / Q000134:cerebrospinal fluid* / Q000737:chemistry*; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D015153:Blotting, Western; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values
chemical_list: D016564:Amyloid beta-Protein Precursor; D053829:Amyloid Precursor Protein Secretases; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C509284:BACE2 protein, human; C509280:BACE1 protein, human