title: Respiratory pathways and oxygen toxicity in Phanerochaete chrysosporium.
abstract: Phanerochaete chrysosporium maintained on glucose as the carbon source contained severely impaired mitochondria that were characterised by the loss of both succinate dehydrogenase and cytochrome oxidase activities. These cells maintained a constant value for energy charge using anaerobic metabolism. Cells with these properties express lignin peroxidase when supplied with a pure oxygen atmosphere, which may reflect a response to accumulating reactive oxygen species. Cells maintained on cellulose retained fully functional mitochondria, but expressed lignin peroxidase without being exposed to a pure oxygen atmosphere. In the cells maintained on cellulose, mitochondrial function may be limited by the supply of glucose, leading to the accumulation of reactive oxygen species.
authors.affiliation: School of Chemical and Life Sciences, University of Greenwich, Wellington Street, London, UK.
authors.forename: L
authors.initials: L
authors.lastname: Zacchi
authors.forename: J M
authors.initials: JM
authors.lastname: Palmer
authors.forename: P J
authors.initials: PJ
authors.lastname: Harvey
mesh_terms: D002482:Cellulose / Q000378:metabolism; D003470:Culture Media; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000633:toxicity*; D010101:Oxygen Consumption; D010544:Peroxidases / Q000096:biosynthesis; D020075:Phanerochaete / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D003470:Culture Media; D002482:Cellulose; D019802:Succinic Acid; D010544:Peroxidases; C042858:lignin peroxidase; D003576:Electron Transport Complex IV; D005947:Glucose; D010100:Oxygen