Stricture formation in Crohn's disease: the role of intestinal fibroblasts.
OBJECTIVE
To determine whether intestinal fibroblasts in patients with Crohn's disease (CD) have an enhanced capacity to reorganize collagen and thus cause stricture formation.


BACKGROUND
Stricture formation is a characteristic feature of CD that may distinguish it from other forms of inflammatory bowel disease. Methods Fibroblasts were obtained at surgery from the colon and ileum of patients with CD and ulcerative colitis (UC) and control patients. Primary fibroblast cultures were obtained by explant technique. Fibroblast contractile activity was measured using fibroblast-populated collagen lattices (FPCLs), in which the cultured fibroblasts were seeded in free-floating collagen gel matrices that they reorganize and contract. Fibroblast contractile activity was measured as the reduction of surface area (mm2) of collagen gel matrix at 24-hour intervals for 1 week.


RESULTS
Fibroblasts from patients with CD displayed enhanced capacity to contract FPCL when compared to UC and control fibroblasts. This activity was maximal in fibroblasts recovered from strictured regions in CD. Fibroblasts from patients with UC had a contractile capacity similar to that of controls. Hydrocortisone inhibited this in vitro contractile activity in a dose-dependent manner.


CONCLUSIONS
Intestinal fibroblasts in CD possess enhanced capacity for collagen reorganization and contractile activity in vitro. This activity may be responsible for stricture formation in CD.
Regan
M C
MC
Department of Surgery, Mater Misericordiae Hospital, Dublin, Ireland.
Flavin
B M
BM
Fitzpatrick
J M
JM
O'Connell
P R
PR
D002460:Cell Line; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000473:pathology / Q000601:surgery; D003094:Collagen / Q000378:metabolism; D003106:Colon / Q000473:pathology / Q000601:surgery; D003424:Crohn Disease / Q000473:pathology*; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D007082:Ileum / Q000473:pathology / Q000601:surgery; D007415:Intestinal Obstruction / Q000473:pathology* / Q000601:surgery
D003094:Collagen