title: Telomere structure regulates the heritability of repressed subtelomeric chromatin in Saccharomyces cerevisiae.
abstract: Telomeres, the protein-DNA structures present at the termini of linear chromosomes, are capable of conferring a reversible repression of Pol II- and Pol III-transcribed genes positioned in adjacent subtelomeric regions. This phenomenon, termed telomeric silencing, is likely to be the consequence of a more global telomere position effect at the level of chromatin structure. To understand the role of telomere structure in this position effect, we have developed an assay to distinguish between the heritability of transcriptionally repressed and derepressed states in yeast. We have previously demonstrated that an elongated telomeric tract leads to hyperrepression of telomere-adjacent genes. We show here that the predominant effect of elongated telomeres is to increase the inheritance of the repressed state in cis. Interestingly, the presence of elongated telomeres overcomes the partial requirement of yCAF-1 in silencing. We propose that the formation of a specific telomeric structure is necessary for the heritability of repressed subtelomeric chromatin.
authors.affiliation: Department of Biochemistry, Tulane University Medical Center, New Orleans, Louisiana 70112, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Park
authors.forename: A J
authors.initials: AJ
authors.lastname: Lustig
mesh_terms: D002843:Chromatin / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D016615:Telomere; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin