title: Prevalence and genetic pattern of feline coronaviruses in urban cat populations.
abstract: The prevalence and phylogeny of feline coronaviruses were studied in urban cat populations by sampling of 113 clinically healthy cats. Rectal swab samples were subjected to a nested reverse-transcription polymerase chain reaction, specific for the conservative nucleocapsid region of the virus genome. More than 30% of the sampled animals proved positive for the presence of feline coronaviruses. The nucleotide sequences of amplified 440 bp products were determined, aligned and the phylogenetic analysis revealed noticeable genetic clusters among the prevalent feline coronaviruses in the surveyed geographic area. These findings will hopefully contribute to the elucidation of the epidemiology of feline infectious peritonitis.
authors.affiliation: Veterinary Institute of Debrecen, P.O. Box 51, H-4002 Debrecen, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Kiss
authors.forename: S
authors.initials: S
authors.lastname: Kecskeméti
authors.forename: J
authors.initials: J
authors.lastname: Tanyi
authors.forename: B
authors.initials: B
authors.lastname: Klingeborn
authors.forename: S
authors.initials: S
authors.lastname: Belák
mesh_terms: D000818:Animals; D002415:Cats / Q000821:virology*; D017934:Coronavirus / Q000235:genetics* / Q000302:isolation & purification*; D018076:DNA, Complementary / Q000737:chemistry; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012007:Rectum / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014505:Urban Population
chemical_list: D018076:DNA, Complementary