[Effect of the protein kinase C inhibitor Ro 31-8220 on Ca2+-responses, induced by somatotropin, in swine granulosa cells].
Somatotropin effect on Ca2+ responses in pig granulosa cells from antral follicles was investigated using fluorescent dye Fluo-3 AM and chlortetracycline. Ro 31-8220 increased the entry of extracellular calcium and the exit of calcium from intracellular stores. In Ca-free medium Ro 31-8220 exerted no influence on the level of calcium in granulosa cells. The effect of somatotropin on pig granulosa cells is associated with PKC activation. These data suggest the involvement of PKC in the changes of calcium in pig granulosa cells activated by somatotropin.
Kuz'mina
T I
TI
All Russian Research Institute of Farm Animal Genetics and Breeding, St. Petersburg-Pushkin.
Denisenko
V Iu
VIu
Shapiev
I Sh
ISh
D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D005456:Fluorescent Dyes; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013552:Swine; D014966:Xanthenes
D000814:Aniline Compounds; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007211:Indoles; D014966:Xanthenes; C059715:Fluo-3; D013006:Growth Hormone; D011493:Protein Kinase C; D002118:Calcium; C064758:Ro 31-8220