Hybridization kinetics of oligodeoxyribonucleotides with a d(GCGAAGC) hairpin at the 3'-end.
In order to protect them against enzymatic attack of serum in the antisense strategy, oligodeoxyribonucleotides can be protected on their 3'-side by the sequence d(GCGAAGC) which spontaneously forms a hairpin which is known for its extraordinary stability with regard to thermal denaturation or nuclease degradation (I. Hirao, G. Kawai, S. Yoshizawa, Y. Nishimura, Y. Ishido, K. Watanabe and K. Miura, Nucleic Acids Res. 22, 576-582 (1994)). By contrast, the hairpin does not prevent hybridization of the 5'-stem part of the oligonucleotide to a target DNA strand. As soon as this pairing occurs, the stability of the hairpin is disrupted. Its opening rate, followed by its pairing if possible, is of the same order than that of hybridization of the stem part.
Chraïbi
Z
Z
Laboratoire d'immunologie et endocrinologie, Université Cadi Ayyad, Marrakech, Morocco.
Réfrégiers
M
M
Jollès
B
B
Laigle
A
A
D001483:Base Sequence; D004735:Energy Transfer; D006358:Hot Temperature; D006860:Hydrogen Bonding; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D009838:Oligodeoxyribonucleotides