Hexamethylenebisacetamide modulation of thyroglobulin and protein levels in thyroid cells is not mediated by phosphatidylinositol-3-kinase: a study with wortmannin.
Hexamethylenebisacetamide (HMBA) induces in murine erythroleukemia cells (MELC) the commitment to terminal differentiation leading to globin gene expression. In the thyroid, HMBA acts as a growth factor and also as a differentiating agent. In the present paper, we studied the effect of HMBA on the very specific thyroid marker thyroglobulin (Tg) in two different thyroid cell systems, i.e., porcine cells in primary culture and ovine cells in long term culture. Using wortmannin, a specific inhibitor of phosphatidylinositol-3-kinase, we investigated whether this enzyme is involved in HMBA mode of action. We found that HMBA is a positive modulator of Tg production in porcine cells, but a negative effector in the OVNIS cell line. As all HMBA effects studied in the present paper, i.e., Tg production and total protein levels, are not inhibited by wortmannin, we suggest the non-involvement of phosphatidylinositol-3-kinase in HMBA mode of action.
Aouani
A
A
Faculté de Medecine, Laboratoire de Recherches en Nutrition U 260 INSERM, Marseille, 13385, France. Mohamed.Amri.@fst.rnu.tn
Samih
N
N
Amphoux-Fazekas
T
T
Hovsépian
S
S
Fayet
G
G
D000081:Acetamides / Q000494:pharmacology*; D000730:Androstadienes / Q000494:pharmacology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012756:Sheep; D015398:Signal Transduction; D013552:Swine; D013954:Thyroglobulin / Q000096:biosynthesis* / Q000235:genetics; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D000077191:Wortmannin
D000081:Acetamides; D000730:Androstadienes; D004791:Enzyme Inhibitors; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011506:Proteins; D013954:Thyroglobulin; D000077191:Wortmannin; C014026:hexamethylene bisacetamide