Phosphate inhibits acetotrophic methanogenesis on rice roots.
The contribution of acetate- and H(2)/CO(2)-dependent methanogenesis to total CH(4) production was determined in excised washed rice roots by radiolabeling, methyl fluoride inhibition, and stable carbon isotope fractionation. Addition of > or = 20 mM phosphate inhibited methanogenesis, which then was exclusively from H(2)/CO(2). Otherwise, acetate contributed about 50 to 60% of the total methanogenesis, demonstrating that phosphate specifically inhibited acetotrophic methanogens on rice roots.
Conrad
R
R
Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany. conrad@mailer.uni-marburg.de
Klose
M
M
Claus
P
P
D000085:Acetates / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D019605:Euryarchaeota / Q000187:drug effects / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology*; D010710:Phosphates / Q000494:pharmacology*; D018517:Plant Roots / Q000382:microbiology*
D000085:Acetates; D002250:Carbon Radioisotopes; D010710:Phosphates; D002245:Carbon Dioxide; D006859:Hydrogen; D008697:Methane