Characterization and chromosome location of the mouse link protein gene (Crtl1).
Link protein (LP) plays an essential role in endochondral bone formation by stabilizing the supramolecular assemblies of aggrecan and hyaluronan. We have isolated and characterized the mouse link protein gene (Crtl1). It is longer than 40 kb and transcribed from two alternative promoters, leading to heterogenous mRNAs between 5.3 and 1.3 kb in size. Apart from the coding sequence, the 5' flanking region is also highly conserved in mammals. Immunostaining revealed high levels of LP expression in the cartilaginous primordia of skeletal elements and low levels in other tissues. Using single-strand conformation polymorphism analysis, Crtl1 was assigned to mouse chromosome 13, tightly linked to Dhfr.
Deák
F
F
Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary.
Mátés
L
L
Krysan
K
K
Liu
Z
Z
Szabó
P E
PE
Mann
J R
JR
Beier
D R
DR
Kiss
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism; D005091:Exons / Q000235:genetics; D016326:Extracellular Matrix Proteins; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011509:Proteoglycans; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D000099249:Proteoglycan Link Protein
D016326:Extracellular Matrix Proteins; D011506:Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D013762:Tetrahydrofolate Dehydrogenase; D000099249:Proteoglycan Link Protein