Efficient expression of foreign proteins in roots from tobravirus vectors.
Viral vectors were constructed from infectious cDNA clones of each of the three tobraviruses, tobacco rattle virus (TRV), pea early-browning virus (PEBV), and pepper ringspot virus (PepRSV). RNA2 of each of the three viruses was modified to carry an additional coat protein subgenomic promoter and was used to express green fluorescent protein (GFP) when inoculated to plants. The tobravirus expression vectors have a wide host range and were able to express GFP in, for example, Nicotiana species, tomato, pea, arabidopsis, and sugar beet. The TRV vector was able to invade and express GFP very efficiently in roots, whereas the widely used PVX vector was not.
MacFarlane
S A
SA
Virology Department, Scottish Crop Research Institute, Invergowrie, DD2 5DA, United Kingdom. s.macfarlane.scri.sari.ac.uk
Popovich
A H
AH
D001709:Biotechnology; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D018517:Plant Roots / Q000378:metabolism / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000821:virology
D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins