title: [Analysis of a case of severe congenital toxoplasmosis].
abstract: BACKGROUND
  To describe a case of severe congenital toxoplasmosis because of inadequate surveillance of a seronegative pregnant woman and to evaluate the usefulness of different microbiological diagnostic methods after birth.
  
  
  METHODS
  We applied serology, DNA amplification by one-tube semi-nested PCR, cell culture and mice inoculation analysis.
  
  
  RESULTS
  Anti. T. gondii serology was useful for the diagnosis of congenital toxoplasmosis. PCR analysis of neonate cerebrospinal fluid and peripheral blood were positive, and yielded negative results after a few days of specific treatment. Cellular culture and mice inoculation yielded negative results.
  
  
  CONCLUSIONS
  Our results suggest that serology and PCR are useful methods for the diagnosis of toxoplasmosis in newborns. Prenatal toxoplasmosis screening and suitable follow up of the seronegative pregnant women are necessary to prevent cases of severe infection in our area.
authors.affiliation: Servicio de Microbiología, Hospital Clínic, Facultat de Medicina, Universitat de Barcelona.
authors.forename: M
authors.initials: M
authors.lastname: Pujol-Riqué
authors.forename: L
authors.initials: L
authors.lastname: Gómez-López
authors.forename: G
authors.initials: G
authors.lastname: Tudó
authors.forename: T
authors.initials: T
authors.lastname: Falgueras
authors.forename: J
authors.initials: J
authors.lastname: Costa
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez-Miguélez
authors.forename: O
authors.initials: O
authors.lastname: Coll
authors.forename: M E
authors.initials: ME
authors.lastname: Valls
mesh_terms: D000208:Acute Disease; D000287:Administration, Topical; D000328:Adult; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D000913:Antibodies, Protozoan / Q000032:analysis; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D001769:Blood / Q000382:microbiology; D001921:Brain / Q000000981:diagnostic imaging; D002555:Cerebrospinal Fluid / Q000382:microbiology; D002825:Chorioretinitis / Q000175:diagnosis / Q000209:etiology; D003907:Dexamethasone / Q000008:administration & dosage; D005260:Female; D005938:Glucocorticoids; D006801:Humans; D007231:Infant, Newborn; D002955:Leucovorin / Q000627:therapeutic use; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use; D051379:Mice; D015997:Neonatal Screening; D009883:Ophthalmic Solutions; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011739:Pyrimethamine / Q000627:therapeutic use; D019105:Random Amplified Polymorphic DNA Technique; D013411:Sulfadiazine / Q000627:therapeutic use; D014057:Tomography, X-Ray Computed; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification; D014125:Toxoplasmosis, Congenital / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000188:drug therapy; D014126:Toxoplasmosis, Ocular / Q000151:congenital* / Q000175:diagnosis
chemical_list: D000893:Anti-Inflammatory Agents; D000913:Antibodies, Protozoan; D000981:Antiprotozoal Agents; D005938:Glucocorticoids; D009883:Ophthalmic Solutions; D013411:Sulfadiazine; D003907:Dexamethasone; D002955:Leucovorin; D008775:Methylprednisolone; D011739:Pyrimethamine