title: The topological organization of chromosomes 9 and 22 in cell nuclei has a determinative role in the induction of t(9,22) translocations and in the pathogenesis of t(9,22) leukemias.
abstract: The neighborhood relationships of chromosomes can be of great importance for basic cellular processes such as gene expression or translocation induction. In this study, the topological organization of chromosomes 9 and 22 was investigated in cell nuclei of G0-phase lymphocytes. We found that the territories of both chromosomes are predominantly located in the central region of cell nuclei. In addition to this, chromosomes 9 and 22 were frequently associated in pairs detected as false-positive ABL-BCR fusions. Both effects might substantially increase the probability of interaction between chromosomes. Because of this, exchange aberrations were studied in chromosomes 9 and 22 of human lymphocytes irradiated by neutrons. The rate of aberration induction between these chromosomes was 11 times higher than the expected frequency based on the fractional molecular weight of these chromosomes. We show that the increased rate of exchange between chromosomes 9 and 22 induced by neutrons corresponds to the neighborhood relationships of both chromosomes. Similar topological characteristics of ABL and BCR genes were found in several cell lines: T- and B-lymphocytes. HL60 cells and bone marrow cells. This finding suggests that the specific chromatin structure mentioned might be responsible for the high rate of induction of t(9;22)-positive leukemias in the human population.
authors.affiliation: Institute of Biophysics, Academy of Sciences, Brno, Czech Republic. kozubek@ibp.cz
authors.forename: S
authors.initials: S
authors.lastname: Kozubek
authors.forename: E
authors.initials: E
authors.lastname: Lukásová
authors.forename: A
authors.initials: A
authors.lastname: Marecková
authors.forename: M
authors.initials: M
authors.lastname: Skalníková
authors.forename: M
authors.initials: M
authors.lastname: Kozubek
authors.forename: E
authors.initials: E
authors.lastname: Bártová
authors.forename: V
authors.initials: V
authors.lastname: Kroha
authors.forename: E
authors.initials: E
authors.lastname: Krahulcová
authors.forename: J
authors.initials: J
authors.lastname: Slotová
mesh_terms: D019677:Artificial Gene Fusion; D001854:Bone Marrow Cells / Q000502:physiology; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics*; D002869:Chromosome Aberrations; D002892:Chromosomes, Human, Pair 22 / Q000737:chemistry / Q000648:ultrastructure; D002899:Chromosomes, Human, Pair 9 / Q000737:chemistry / Q000648:ultrastructure; D016313:Genes, abl; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007938:Leukemia / Q000235:genetics*; D008214:Lymphocytes / Q000502:physiology / Q000528:radiation effects; D008297:Male; D009502:Neutrons; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051562:Proto-Oncogene Proteins c-bcr; D014178:Translocation, Genetic
chemical_list: D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C496613:BCR protein, human; D051562:Proto-Oncogene Proteins c-bcr