Novel metabolic pathway of estrone and 17beta-estradiol catalyzed by cytochrome P-450.
We have already reported that the quinol formation from some para-alkylphenols, which is a novel metabolic pathway catalyzed by cytochrome P-450, occurs in a rat liver microsomal system (). In the present study, we investigated whether estrone and 17beta-estadiol, each of which contains a p-alkylphenol moiety, are also oxidized into the corresponding quinols by cytochrome P-450. Six recombinant human cytochrome P-450 enzymes, CYP1A1, CYP1A2, CYP2B6, CYP2C9, CYP2E1, and CYP3A4, were tested. The results show that estrone and 17beta-estadiol were converted into the corresponding quinols by CYP1A1, CYP2B6, and CYP2E1.
Ohe
T
T
Faculty of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.
Hirobe
M
M
Mashino
T
T
D002384:Catalysis; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism*; D006801:Humans; D006873:Hydroquinones / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D015456:Leukemia, Biphenotypic, Acute / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
D006873:Hydroquinones; D007527:Isoenzymes; D011994:Recombinant Proteins; D004970:Estrone; D004958:Estradiol; D003577:Cytochrome P-450 Enzyme System