Genetic Engineering of Streptomyces coelicolor A3(2) for the Enantioselective Reduction of Unnatural beta-Keto-Ester Substrates.
Potential "reagents" for the enantioselective reduction, and other biotransformations, of beta-keto-esters result from the genetic engineering of Streptomyces coelicolor A3(2). For example, incubation of the N-acetylcysteamine thioester 1 with the recombinant strain CH999/pIJ5675 followed by treatment with MeOH/HCl gave the lactone 2 as essentially a single enantiomer.
Anson
CE
School of Chemical Sciences University of East Anglia Norwich NR4 7TJ (UK).
Bibb
MJ
Booker-Milburn
KI
Clissold
C
Haley
PJ
Hopwood
DA
Ichinose
K
Revill
WP
Stephenson
GR
Surti
CM