title: Observation of soft contact lens disinfection with fluorescent metabolic stains.
abstract: A rapid fluorescent staining method using a tetrazolium dye and propidium iodide for the in-use assessment of disinfection of Pseudomonas aeruginosa biofilms on soft contact lenses showed that 11 to 13% of cells on lenses remained actively respiring and recoverable by culture methods after 30 min of exposure to 3% hydrogen peroxide.
authors.affiliation: School of Biological and Biomedical Sciences, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Gavin
authors.forename: N F
authors.initials: NF
authors.lastname: Button
authors.forename: I A
authors.initials: IA
authors.lastname: Watson-Craik
authors.forename: N A
authors.initials: NA
authors.lastname: Logan
mesh_terms: D018441:Biofilms / Q000187:drug effects; D004396:Coloring Agents; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D011419:Propidium; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013777:Tetrazoles; D013778:Tetrazolium Salts
chemical_list: D004396:Coloring Agents; D013777:Tetrazoles; D013778:Tetrazolium Salts; C075453:5-cyano-2,3-ditolyltetrazolium; D011419:Propidium; D006861:Hydrogen Peroxide