title: Molecular, genetic and physiological analysis of Cladosporium resistance gene function in tomato.
abstract: Characterization of the DNA sequence of 4 tomato leaf mould disease resistance genes (Cf-2, Cf-4, Cf-5 and Cf-9) leads to the prediction that they encode C-terminally membrane anchored glycopeptides with many extracytoplasmic leucine rich repeats (LRRs). The N terminal LRRs are variable between the Cf-genes, suggesting a role in specificity, and the C terminal LRRs are more conserved, suggesting a role in signal transduction. Genetic analysis has revealed several Rcr genes that are required for Cf-gene function; their isolation will help us understand how Cf-genes work. Cf-9 confers responsiveness to pathogen-encoded Avr9 peptide on introduction to tobacco. Tobacco suspension cultures carrying the Cf-9 gene produce reactive oxygen species in response to Avr9 peptide, whereas untransformed cultures do not. The significance of these observations is discussed.
authors.affiliation: Sainsbury Laboratory, John Innes Centre, Norwich, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Jones
authors.forename: P
authors.initials: P
authors.lastname: Brading
authors.forename: M
authors.initials: M
authors.lastname: Dixon
authors.forename: K
authors.initials: K
authors.lastname: Hammond-Kosack
authors.forename: K
authors.initials: K
authors.lastname: Harrison
authors.forename: K
authors.initials: K
authors.lastname: Hatzixanthis
authors.forename: M
authors.initials: M
authors.lastname: Parniske
authors.forename: P
authors.initials: P
authors.lastname: Piedras
authors.forename: M
authors.initials: M
authors.lastname: Torres
authors.forename: S
authors.initials: S
authors.lastname: Tang
authors.forename: C
authors.initials: C
authors.lastname: Thomas
authors.forename: J D
authors.initials: JD
authors.lastname: Jones
mesh_terms: D002963:Cladosporium; D015870:Gene Expression; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics*; D010935:Plant Diseases