DNA polymorphism of srRNA gene among Eimeria tenella strains isolated in Japan.
DNA polymorphism in twelve starains of Eimeria tenella isolated from various places in Japan was examined using 1.l kb small subunits ribosomal RNA amplified by PCR. Genetic variation was evaluated by random amplification of polymorphic DNA (RAPD) analysis. DNA fingerprint patterns were grouped into two, indicating that at least two DNA polymorphisms exist in Japanese E. tenella strains.
Tsuji
N
N
National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Ohta
M
M
Kawazu
S
S
Kamio
T
T
Isobe
T
T
Shimura
K
K
Fujisaki
K
K
D000818:Animals; D002645:Chickens; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics*; D016786:Eimeria tenella / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D014644:Genetic Variation / Q000235:genetics*; D007564:Japan; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic / Q000235:genetics; D011201:Poultry Diseases / Q000469:parasitology*; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms
D017931:DNA Primers; D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012335:RNA, Ribosomal