title: Phenotypic and functional properties of gamma delta T cells from patients with Guillain Barré syndrome.
abstract: In this study we have examined the phenotypic and functional properties of circulating gamma delta T cells in patients with Guillain Barre syndrome (GBS), in normal healthy controls, and in patients with active multiple sclerosis (MS). Cells expressing the Vdelta2 T cell receptor showed elevated expression of the C-lectin receptor NKRP1A in both GBS and MS, suggestive of an activated state. However, in patients with GBS these cells failed to respond to pyrenil-pyrophosphate derivatives and Vdelta2 + T cell clones derived from these patients released lower levels of IFNgamma than Vdelta2 + clones derived from controls and MS patients. In contrast, in patients with GBS the Vdelta1 + subset was expanded, showed elevated expression of NKRPIA and Vdelta1 + clones derived from these patients secreted high levels of IL-4. Our findings of expanded NKRP-1A +, IL-4-producing Vdelta1 T cells in the GBS patients suggests the possibility that these cells are activated by the recognition of non-protein antigens in an MHC-unrestricted manner and contribute to the humoral response to glycolipids that is a hallmark of this disease.
authors.affiliation: Laboratory of Neuroimmunology, IRCCS Santa Lucia, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Borsellino
authors.forename: F
authors.initials: F
authors.lastname: Poccia
authors.forename: R
authors.initials: R
authors.lastname: Placido
authors.forename: D
authors.initials: D
authors.lastname: Tramonti
authors.forename: G
authors.initials: G
authors.lastname: Mancino
authors.forename: S
authors.initials: S
authors.lastname: Luchetti
authors.forename: S
authors.initials: S
authors.lastname: Galgani
authors.forename: B
authors.initials: B
authors.lastname: Bonetti
authors.forename: S
authors.initials: S
authors.lastname: Bach
authors.forename: B
authors.initials: B
authors.lastname: Cipriani
authors.forename: C F
authors.initials: CF
authors.lastname: Brosnan
authors.forename: L
authors.initials: L
authors.lastname: Battistini
mesh_terms: D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism; D001773:Blood Cells / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D020275:Guillain-Barre Syndrome / Q000097:blood*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism; D037181:Lectins, C-Type; D008024:Ligands; D009103:Multiple Sclerosis / Q000097:blood; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010641:Phenotype; D010766:Phosphorylation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D055607:Receptors, Natural Killer Cell; D012016:Reference Values; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology*
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; C527203:KLRB1 protein, human; D037181:Lectins, C-Type; D008024:Ligands; D055651:NK Cell Lectin-Like Receptor Subfamily B; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell