Partial liquid ventilation with perfluorocarbon improves gas exchange and decreases inflammatory response in oleic acid-induced lung injury in beagles.
The aim of this study was to determine the effect of partial liquid ventilation (PLV) using a perfluorocarbon (PFC) on gas exchange and lung inflammatory response in a canine acute lung injury model. After inducing severe lung injury by oleic acid infusion, beagle dogs were randomized to receive either gas ventilation only (control group, n = 6) or PLV (PLV group, n = 7) by sequential instillation of 10 mL/kg of perfluorodecalin (PFC) at 30 min intervals till functional residual capacity was attained. Measurements were made every 30 min till 210 min. Then the lungs were removed and bronchoalveolar lavage (BAL) (35 mL/kg) was performed on the right lung and the left lung was submitted for histologic analysis. There was significant improvement in PaO2 and PaCO2 in the PLV group compared to the control group (p < 0.05) which was associated with a significant decrease in shunt (p < 0.05). There was no significant difference in parameters of lung mechanics and hemodynamics. There was a significant decrease in cell count and neutrophil percentage in BAL fluid and significantly less inflammation and exudate scores in histology in the PLV group (p < 0.05). We conclude that PLV with perfluorodecalin improves gas exchange and decreases inflammatory response in the acutely-injured lung.
Suh
G Y
GY
Department of Medicine, Sungkyunkwan University School of Medicine, Seoul, Korea.
Chung
M P
MP
Park
S J
SJ
Park
J W
JW
Kim
H C
HC
Kim
H
H
Han
J
J
Rhee
C H
CH
Kwon
O J
OJ
D000818:Animals; D001772:Blood Cell Count; D001992:Bronchoalveolar Lavage Fluid; D002245:Carbon Dioxide / Q000032:analysis; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D005466:Fluorocarbons / Q000494:pharmacology*; D006439:Hemodynamics; D006651:Histocytochemistry; D007249:Inflammation / Q000517:prevention & control; D008171:Lung Diseases / Q000139:chemically induced / Q000503:physiopathology*; D008297:Male; D019301:Oleic Acid; D010100:Oxygen / Q000032:analysis; D011659:Pulmonary Gas Exchange / Q000187:drug effects*; D012123:Pulmonary Ventilation / Q000502:physiology*; D012129:Respiratory Function Tests; D012122:Ventilators, Mechanical
D005466:Fluorocarbons; D002245:Carbon Dioxide; D019301:Oleic Acid; C008805:perfluorodecalin; D010100:Oxygen