title: A conserved motif in Crumbs is required for E-cadherin localisation and zonula adherens formation in Drosophila.
abstract: BACKGROUND
  Specialised cell junctions in epithelia serve as cell-cell adhesion sites and thus contribute to the maintenance of tissue integrity. The Drosophila gene crumbs encodes a transmembrane protein that is required for the biogenesis of the zonula adherens, a belt-like structure encircling the apex of epithelial cells. As previously shown, expression of just the short membrane-bound cytoplasmic domain is sufficient to rescue major defects associated with the loss of crumbs function.
  
  
  RESULTS
  The cytoplasmic domain of Crumbs is highly conserved in two putative crumbs homologues in Caenorhabditis elegans. To assess the significance of conserved residues, various point mutations and deletions were introduced into this region. Two functional domains were revealed, an amino-terminal region and the carboxy-terminal amino acids EERLI. Both are necessary for rescue of the crumbs phenotype. The EERLI motif interacts with Discs Lost, a cytoplasmic protein containing PDZ domains. Overexpression of the Crumbs cytoplasmic domain induces a transition from the single-layered epithelium to a multilayered tissue. This transition is associated with redistribution of the Drosophila homologue of the cell adhesion molecule E-cadherin, and depends on the presence of the EERLI motif.
  
  
  CONCLUSIONS
  We propose a model in which the interaction of the Crumbs carboxyl terminus with Discs Lost organises a membrane-associated protein complex in the apical cytocortex of epithelial cells. This scaffold mediates the localisation and stabilisation of the zonula adherens component DE-cadherin, a crucial component for the maintenance of epithelial cell polarity and tissue integrity.
authors.affiliation: Institut für Genetik, Heinrich-Heine Universität Düsseldorf, Düsseldorf, 40225, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Klebes
authors.forename: E
authors.initials: E
authors.lastname: Knust
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015820:Cadherins / Q000378:metabolism*; D017124:Conserved Sequence; D003593:Cytoplasm / Q000378:metabolism; D017931:DNA Primers; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D007365:Intercellular Junctions; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D017386:Sequence Homology, Amino Acid
chemical_list: D015820:Cadherins; D017931:DNA Primers; D029721:Drosophila Proteins; D008565:Membrane Proteins; C064360:crb protein, Drosophila