Plant interstitial telomere motifs participate in the control of gene expression in root meristems.
The promoters of Arabidopsis eEF1A genes contain a telomere motif, the telo-box, associated with an activating sequence, the tef-box. Database searches indicated the presence of telo-boxes in the 5' region of numerous genes encoding components of the translational apparatus. By using several promoter constructs we demonstrate that the telo-box is required for the expression of a beta-glucoronidase gene in root primordia of transgenic Arabidopsis. This effect was observed when a telo-box was inserted upstream or downstream from the transcription initiation site, and occurred in synergy with the tef-box. These results clearly indicate that interstitial telomere motifs in plants are involved in control of gene expression. South-western screening of a lambdaZAP library with a double-stranded Arabidopsis telomere motif resulted in characterization of a protein related to the conserved animal protein Puralpha. The possibility of a regulation process similar to that achieved by the Rap1p in Saccharomyces cerevisiae is discussed.
Tremousaygue
D
D
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27, 31326 Castanet-Tolosan, France.
Manevski
A
A
Bardet
C
C
Lescure
N
N
Lescure
B
B
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D002875:Chromosomes / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D018519:Meristem / Q000502:physiology; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016615:Telomere / Q000235:genetics*
D005966:Glucuronidase