Effect of three diaryl tellurides, and an organoselenium compound in trout erythrocytes exposed to oxidative stress in vitro.
Previous literature reports have demonstrated that nucleated trout erythrocytes in conditions of oxidative stress are subjected to DNA and membrane damage, and inactivation of glutathione peroxidase. The present study was undertaken to evaluate the ability of three diaryl tellurides and the organoselenium compound ebselen to protect trout (Salmo irideus) erythrocytes against oxidative stress, induced thermally and by a variation of pH. The antioxidant ability of these molecules was evaluated through chemiluminescence. Impairment of DNA was assessed using the comet assay, a rapid and sensitive single cell gel electrophoresis technique, used to detect primary DNA damage in individual cells. At low concentrations (<10 microM), all the compounds used presented a protective effect on DNA damage without altering the hemolysis rate. In higher concentrations, they accelerated the hemolysis rate and two of the diaryl tellurides were strongly genotoxic.
Tiano
L
L
Dipartimento di Biologia Molecolare, Cellulare e Animale, Università degli Studi di Camerino, Via Camerini 2, I-62032, Camerino, Italy. l.tiano@cambio.unicam.it
Fedeli
D
D
Santroni
A M
AM
Villarini
M
M
Engman
L
L
Falcioni
G
G
D000818:Animals; D000975:Antioxidants / Q000633:toxicity*; D001393:Azoles / Q000633:toxicity*; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D006358:Hot Temperature / Q000009:adverse effects; D006863:Hydrogen-Ion Concentration; D054833:Isoindoles; D008163:Luminescent Measurements; D009152:Mutagenicity Tests; D017686:Oncorhynchus mykiss; D009942:Organometallic Compounds / Q000737:chemistry / Q000633:toxicity*; D016566:Organoselenium Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D013691:Tellurium / Q000737:chemistry
D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D009942:Organometallic Compounds; D016566:Organoselenium Compounds; C042986:ebselen; D013691:Tellurium