title: Polarized distribution of glycine transporter isoforms in epithelial and neuronal cells.
abstract: Asymmetrical distribution of Na(+)- and Cl(-)-dependent neurotransmitter transporters on the cell surface of polarized cells seems to be a generalized feature in this gene family. In the present study we analyzed the subcellular distribution of the various isoforms of the glycine transporters GLYT1 and GLYT2 after heterologous expression in polarized MDCK cells and in hippocampal neurons. Our results indicate that glycine transporters are asymmetrically distributed in an isoform- and cell-type-specific manner. GLYT1b is localized in the basolateral and somatodendritic domains of MDCK cells and neurons, respectively. However, GLYT1a is somatodendritic in neurons but is predominantly expressed in the apical surface of MDCK cells. The two isoforms of GLYT2 (GLYT2a and GLYT2b) are found at the apical surface in epithelial cells but are uniformly distributed in neurons. By using site-directed mutagenesis we have been able to identify signals for basolateral/somatodendritic localization in the amino-terminal region of GLYT1 and in two dileucine motifs located in the carboxyl tail of this protein. These results contribute to defining the mechanisms of asymmetrical distribution of transporters on the cell surface of polarized cells.
authors.affiliation: Centro de Biología Molecular "Severo Ochoa," Facultad de Ciencias, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas, Madrid, 28049, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Poyatos
authors.forename: F
authors.initials: F
authors.lastname: Ruberti
authors.forename: R
authors.initials: R
authors.lastname: Martínez-Maza
authors.forename: C
authors.initials: C
authors.lastname: Giménez
authors.forename: C G
authors.initials: CG
authors.lastname: Dotti
authors.forename: F
authors.initials: F
authors.lastname: Zafra
mesh_terms: D000595:Amino Acid Sequence; D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics*; D002460:Cell Line; D016764:Cell Polarity; D002478:Cells, Cultured; D004285:Dogs; D004622:Embryo, Mammalian; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D050487:Glycine Plasma Membrane Transport Proteins; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D051381:Rats; D016415:Sequence Alignment
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D050487:Glycine Plasma Membrane Transport Proteins; D020033:Protein Isoforms; C493269:Slc6a5 protein, rat; C493266:Slc6a9 protein, rat