Detection of peptide nucleic acids in tissue extracts of treated animals by gel mobility shift assay.
We have developed a sensitive and reproducible gel mobility shift assay to detect PNA oligomers in tissue of treated animals. PNA present in purified tissue extracts of treated animals is hybridized to a 33P-labelled DNA oligomer probe, and analyzed by polyacrylamide gel electrophoresis. The PNA-DNA hybrid migrates more slowly than the DNA probe alone and can be quantified relative to a standard curve. This detection method is useful for detecting PNAs in many different tissues, including brain, heart, kidney, liver, spleen, and serum, as well as cells in culture.
Jansen
K
K
Laboratory of Neuropsychopharmacology, Mayo Foundation for Medical and Educational Research, Jacksonville, FL 32224, USA. jansen.karen@mayo.edu
Richelson
E
E
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000032:analysis* / Q000494:pharmacology; D018028:Receptors, Neurotensin / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014018:Tissue Distribution; D014020:Tissue Extracts / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D018028:Receptors, Neurotensin; D014020:Tissue Extracts