title: Kinetic analysis of the binding of Ulex europeas agglutinin 1 (UEA 1) to H-antigenic fucolipid.
abstract: The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several order of magnitude slower than the diffusion controlled reactions. Analyses of activation parameters reveals that these slow second-order rate constants are a consequence of an unfavorable activation enthalpy term.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560 012, India.
authors.forename: C J
authors.initials: CJ
authors.lastname: Thomas
authors.forename: A
authors.initials: A
authors.lastname: Surolia
mesh_terms: D000017:ABO Blood-Group System / Q000737:chemistry* / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes; D037121:Plant Lectins; D011485:Protein Binding; D013816:Thermodynamics
chemical_list: D000017:ABO Blood-Group System; D006017:Glycolipids; C102547:H1 blood group antigen; D037102:Lectins; D008081:Liposomes; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C025567:fucolipids