[Quick RNA extraction from specified morphological region in frozen sections and its RT-PCR application].
OBJECTIVE
To establish a method for quick RNA extraction from specified histological region in frozen sections so as to avoid cross RNA contamination among different types of cells/tissues and to achieve specific RT-PCR amplification.


METHODS
Prepare serial frozen sections 5microm in thickness for histological and immunocytochemical examination. Based on the results, make one or two pieces of 20 microm section and select a specified region for quick RNA extraction and RT-PCR analysis.


RESULTS
RNA extraction can be finished within an hour and the data of RT-PCR obtained within 6 hours. Besides the high performance, this method can efficiently exclude pseudo positive or pseudo negative results caused by inter-cellular RNA contamination.


CONCLUSIONS
This method is an important complement to the current techniques for RNA extraction and RT-PCR.
Jang
Y
Y
Division of Molecular Biology, Shenyang Medical College, Shenyang, Liaoning, 110031 P.R. China. lihongm@pub.sy.ln.net.cn
Liu
N
N
Liu
J
J
Li
H
H
D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry; D012313:RNA / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012313:RNA