title: Open field locomotion and neurotransmission in mice evaluated by principal component factor analysis-effects of housing condition, individual activity disposition and psychotropic drugs.
abstract: Effects of housing condition and individual disposition on dopaminergically and GABAergically influenced open field locomotion and neurochemistry were studied in mice. Mice characterized as high active (HAM) and low active (LAM) by a running-wheel test were housed in groups or isolated for 1 day, 1 week, 3, 6, 12 or 18 weeks before an open field test was performed with saline, apomorphine (0.75 mg/kg) or diazepam (1.00 mg/kg) administration. Immediately afterwards animals were decapitated and brain sections were frozen for subsequent HPLC-analysis of dopaminergic and serotonergic transmitter metabolism. Principal component factor analysis (PCA) of locomotion variables provided three factors explaining 78.5% of total variance. Variables related to the amount of locomotion loaded highly on Factor 1 (F1-Activity), variables related to place utilization loaded highly on Factor 2 (F2-Exploration) and variables related to immobility and place preference loaded highly on Factor 3 (F3-Irritation). Apomorphine decreased F1-Activity with smaller effects in HAM and without changes in F2-Exploration and F3-Irritation independent on housing conditions. Diazepam exerted a decrease in F2-Exploration with a small increase in FI-Activity and no effects in F3-Irritation. Diazepam induced changes depended on housing conditions and were especially pronounced in isolated HAM. PCA of considerable locomotion and neurochemical data revealed interrelationships between striatal dopamine metabolism and F1-Activity, between cortical dopamine and serotonin metabolism and F2-Exploration as well as between cerebellar, hippocampal and striatal serotonin metabolism and F3-Irritation. The authors concluded that the application of PCA is a useful method to provide functionally relevant characteristics of behaviors and functionally relevant descriptions of interrrelationships between behavior and appropriate central nervous mechanisms. Furthermore the received behavioral characteristics (F1, F2, F3) of open field locomotion were sensitive to reveal housing and drug effects.
authors.affiliation: Department of Psychiatry, Technical University Dresden, Germany. Monika.Jaehkel@mailbox.tu-dresden.de
authors.forename: M
authors.initials: M
authors.lastname: Jähkel
authors.forename: O
authors.initials: O
authors.lastname: Rilke
authors.forename: R
authors.initials: R
authors.lastname: Koch
authors.forename: J
authors.initials: J
authors.lastname: Oehler
mesh_terms: D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D001923:Brain Chemistry / Q000187:drug effects; D003975:Diazepam / Q000494:pharmacology; D004298:Dopamine / Q000502:physiology; D018491:Dopamine Agonists / Q000494:pharmacology; D005163:Factor Analysis, Statistical; D018757:GABA Modulators / Q000494:pharmacology; D006799:Housing, Animal; D007509:Irritants / Q000494:pharmacology; D051379:Mice; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology*; D011619:Psychotropic Drugs / Q000494:pharmacology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D018491:Dopamine Agonists; D018757:GABA Modulators; D007509:Irritants; D011619:Psychotropic Drugs; D005680:gamma-Aminobutyric Acid; D001058:Apomorphine; D003975:Diazepam; D004298:Dopamine