title: Porous balloon delivery of S-dC28 does not prevent restenosis in the porcine coronary artery model of balloon injury.
abstract: Phosphorothioate (PS) oligodeoxynucleotides (ODN) inhibit vascular smooth muscle cell proliferation through antisense and G-quartet aptameric mechanisms. PS-ODN such as the cytidine homopolymers, have been demonstrated to have non-G-quartet, nonsequence-specific inhibitory effects in a rat carotid balloon injury model of neointimal proliferation. We sought to test the efficacy of S-dC28, a cytidine homopolymer lacking G-quartets, on neointimal proliferation in the porcine coronary artery model of balloon injury. A total of 23 animals (11 controls, 12 treated) were subjected to balloon injury in a coronary artery, followed by infusion of control solution or S-dC28 via porous balloon, the Scimed Dispatch Coronary Infusion Catheter. After a mean interval of 49 days, the animals were killed, and the target coronary segments were examined histologically. S-dC28 did not significantly inhibit neointimal formation. Fluorescein isothiocyanate (FITC)-labeled S-dC28 was present in the intima and media immediately after administration but was present mainly within the adventitia 3 hours after administration. S-dC28, when delivered by a Scimed Dispatch Coronary Infusion Catheter (Maple Grove, MN), did not significantly affect neointimal proliferation after balloon injury in a porcine coronary artery model.
authors.forename: A D
authors.initials: AD
authors.lastname: Simon
authors.forename: K N
authors.initials: KN
authors.lastname: Giedd
authors.forename: A
authors.initials: A
authors.lastname: Schwartz
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: H J
authors.initials: HJ
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Marboe
authors.forename: C A
authors.initials: CA
authors.lastname: Stein
authors.forename: L E
authors.initials: LE
authors.lastname: Rabbani
mesh_terms: D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects; D000818:Animals; D002455:Cell Division; D003331:Coronary Vessels / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D004195:Disease Models, Animal; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000235:genetics; D051381:Rats; D013552:Swine; D013873:Thionucleotides / Q000008:administration & dosage / Q000235:genetics
chemical_list: D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D013873:Thionucleotides; D016650:Fluorescein-5-isothiocyanate