Regulation of abscisic acid-induced stomatal closure and anion channels by guard cell AAPK kinase.
Abscisic acid (ABA) stimulates stomatal closure and thus supports water conservation by plants during drought. Mass spectrometry-generated peptide sequence information was used to clone a Vicia faba complementary DNA, AAPK, encoding a guard cell-specific ABA-activated serine-threonine protein kinase (AAPK). Expression in transformed guard cells of AAPK altered by one amino acid (lysine 43 to alanine 43) renders stomata insensitive to ABA-induced closure by eliminating ABA activation of plasma membrane anion channels. This information should allow cell-specific, targeted biotechnological manipulation of crop water status.
Li
J
J
Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA.
Wang
X Q
XQ
Watson
M B
MB
Assmann
S M
SM
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000838:Anions / Q000378:metabolism*; D019470:Biolistics; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004789:Enzyme Activation; D007887:Fabaceae / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant; D007473:Ion Channels / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D018408:Patch-Clamp Techniques; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D010940:Plant Proteins; D010946:Plants, Medicinal; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011523:Protoplasts / Q000201:enzymology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014170:Transformation, Genetic
D000838:Anions; D018076:DNA, Complementary; D007473:Ion Channels; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D000040:Abscisic Acid; D017346:Protein Serine-Threonine Kinases