Cytogenetic mapping of 31 functional genes on chicken chromosomes by direct R-banding FISH.
Using direct R-banding fluorescence in situ hybridization, we determined the location of 31 functional genes on chicken chromosomes. Replication R-banded chromosomes were obtained by synchronizing splenocyte cultures with excessive thymidine, followed by BrdU treatment. Thirty-one functional genes were directly localized to banded chicken chromosomes using genomic DNA and cDNA fragments as probes. The possibility of conserved linkage homology between chicken and human chromosomes was demonstrated for seven chicken chromosome regions (1p, 1q, 2q, 4p, 4q, and 5q).
Suzuki
T
T
Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
Kurosaki
T
T
Shimada
K
K
Kansaku
N
N
Kuhnlein
U
U
Zadworny
D
D
Agata
K
K
Hashimoto
A
A
Koide
M
M
Koike
M
M
Takata
M
M
Kuroiwa
A
A
Minai
S
S
Namikawa
T
T
Matsuda
Y
Y
D000818:Animals; D002645:Chickens / Q000235:genetics*; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000235:genetics; D017124:Conserved Sequence / Q000235:genetics; D015342:DNA Probes; D018076:DNA, Complementary; D019143:Evolution, Molecular; D005796:Genes; D008040:Genetic Linkage / Q000235:genetics; D016678:Genome; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020161:Physical Chromosome Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D018076:DNA, Complementary