title: Establishment of a myelodysplastic syndrome (MDS)/secondary AML-derived T lymphoid cell line K2-MDS.
abstract: We have established a T lymphoid cell line, K2-MDS, from the peripheral blood mononuclear cells (PBMC) of a patient with acute myeloblastic leukemia (AML) transformed myelodysplastic syndrome (MDS). K2-MDS cells are positive for the expression of CD4, CD5, CD13, CD25, CD71, CD95, HLA-DR and cytoplasmic CD3. Southern blotting analysis shows T cell receptor (TCR) beta chain genes rearrangements, whereas immunoglobulin heavy chain (IgH) genes are not rearranged. Further, the patient PBMC contains TCR beta chain genes rearrangements in the same manner as K2-MDS cells. The data indicate that K2-MDS is a T lymphoid cell line derived from a myelodysplastic clone in the patient PBMC. This new MDS-derived cell line K2-MDS may be a useful in vitro model for studies on the pathogenetic mechanisms leading to MDS.
authors.affiliation: Third Department of Internal Medicine, School of Medicine, Kinki University, Osaka-Sayama, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: Y
authors.initials: Y
authors.lastname: Sumimoto
authors.forename: H
authors.initials: H
authors.lastname: Nawata
authors.forename: T
authors.initials: T
authors.lastname: Sano
authors.forename: M
authors.initials: M
authors.lastname: Higashishiba
authors.forename: H
authors.initials: H
authors.lastname: Haga
authors.forename: Y
authors.initials: Y
authors.lastname: Tatsumi
authors.forename: F
authors.initials: F
authors.lastname: Horiuchi
authors.forename: K
authors.initials: K
authors.lastname: Irimajiri
authors.forename: A
authors.initials: A
authors.lastname: Kanamaru
mesh_terms: D000208:Acute Disease; D016207:Cytokines / Q000494:pharmacology; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D016130:Immunophenotyping; D007951:Leukemia, Myeloid / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D016207:Cytokines; D008934:Mitogens