title: Introduction of 6-formylcytidine into a Myb binding sequence.
abstract: Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3'-TTGAC-5'. The modified duplex showed Tm of 67 degrees C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.
authors.affiliation: School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kittaka
authors.forename: T
authors.initials: T
authors.lastname: Kuze
authors.forename: M
authors.initials: M
authors.lastname: Amano
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Miyasaka
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: A
authors.initials: A
authors.lastname: Sarai
authors.forename: T
authors.initials: T
authors.lastname: Yasukawa
authors.forename: S
authors.initials: S
authors.lastname: Ishii
mesh_terms: D001665:Binding Sites; D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000737:chemistry; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013696:Temperature
chemical_list: C403912:6-formylcytidine; D009838:Oligodeoxyribonucleotides; D020598:Proto-Oncogene Proteins c-myb; D003562:Cytidine; D008239:Lysine