title: Brush border myosin I (BBMI): a basally localized transcript in human jejunal enterocytes.
abstract: To extend our recent observation that villin mRNA, encoding an apical microvillous protein, is dichotomously localized in the basal region of human enterocytes, we examined the localization of mRNAs for brush border myosin I (BBMI) and intestinal fimbrin (I-fim). In situ hybridization indicated that BBMI mRNA localized to the basal region of human enterocytes, whereas the mRNA for I-fim distributed diffusely. To facilitate study of potential mechanisms of mRNA targeting, we cloned a full-length cDNA for BBMI including its 5'- and 3'-untranslated regions (UTRs). This cDNA shares 86% sequence identity with bovine BBMI and 85% with rat BBMI. Sequence analysis revealed no obvious similarity between the 3'-UTRs of BBMI and villin. This study provides evidence of novel sorting pathways for intestinal microvillous cytoskeletal proteins.
authors.affiliation: Division of Pediatric Gastroenterology/Nutrition, The Floating Hospital for Children, New England Medical Center Hospitals, Boston, Massachusetts 02111, USA.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: L M
authors.initials: LM
authors.lastname: Coluccio
authors.forename: P
authors.initials: P
authors.lastname: Matsudaira
authors.forename: R J
authors.initials: RJ
authors.lastname: Grand
mesh_terms: D001483:Base Sequence; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D002451:Cell Compartmentation; D016764:Cell Polarity; D003001:Cloning, Molecular; D020895:Enterocytes / Q000648:ultrastructure*; D005784:Gene Amplification; D006801:Humans; D017403:In Situ Hybridization; D007583:Jejunum / Q000648:ultrastructure*; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains; D024461:Myosin Type I; D017422:Sequence Analysis, DNA; D020506:Untranslated Regions
chemical_list: D002148:Calmodulin-Binding Proteins; C062291:MYO1A protein, human; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D020506:Untranslated Regions; C026742:plastin; D024461:Myosin Type I; D018995:Myosin Heavy Chains