Transganglionic transport of the lectin soybean agglutinin (Glycine max) following injection into the sciatic nerve of the adult rat.
The lectin soybean agglutinin (SBA) from Glycine max binds to small-sized dorsal root ganglion cells and their central terminals in the superficial dorsal horn of the spinal cord. Here we investigated the ability of SBA and SBA conjugated to horseradish peroxidase (SBA-HRP) to trace thin calibre afferents into the spinal cord from a peripheral nerve. Following injection into the sciatic nerve, labelled cells in the dorsal root ganglion were predominantly small-sized but some medium-sized cells were also labelled. Colocalization studies of transported SBA with various neuronal markers showed that all neurons that transported SBA-HRP showed SBA binding, indicating high uptake specificity for the conjugate. 15% were immunoreactive for RT97 indicating that some axons were myelinated, and 54% also expressed binding sites for isolectin B4 from Griffonia simplicifolia, a selective marker for a subpopulation of unmyelinated afferents. With regard to neurotransmitter content, 43% of the SBA cells contained calcitonin gene-related peptide, 33% contained substance P and 2.5% somatostatin. In addition, 3% contained carbonic anhydrase. Centrally, injection of SBA in the sciatic nerve resulted in labelled terminals in somatotopically appropriate regions of laminae I-II of the dorsal horn, and in the gracile nucleus. A few neurons in the dorsal horn were labelled indicating that some transneuronal transport of SBA had occurred. The results show that SBA can be used as a transganglionic tracer to label fine calibre primary afferents that project to laminae I-II of the spinal cord and the gracile nucleus. It appears to label more afferents than isolectin B4, including also a subpopulation of myelinated afferents.
Shortland
P J
PJ
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Wang
H F
HF
Molander
C
C
D000367:Age Factors; D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D015415:Biomarkers; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002256:Carbonic Anhydrases / Q000032:analysis; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D037102:Lectins / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D008845:Microinjections; D009475:Neurons, Afferent / Q000737:chemistry / Q000201:enzymology; D037121:Plant Lectins; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D012584:Sciatic Nerve / Q000378:metabolism*; D013004:Somatostatin / Q000032:analysis; D030262:Soybean Proteins; D013373:Substance P / Q000032:analysis; D010835:Phytohemagglutinins
D015415:Biomarkers; D015740:Calcitonin Gene-Related Peptide; D002256:Carbonic Anhydrases; D006735:Horseradish Peroxidase; D037102:Lectins; D037121:Plant Lectins; D013004:Somatostatin; D030262:Soybean Proteins; D013373:Substance P; C028824:soybean lectin; D010835:Phytohemagglutinins