Comparison of blood collected in acid-citrate-dextrose and EDTA for use in human immunodeficiency virus peripheral blood mononuclear cell cultures.
Paired blood samples collected in acid-citrate-dextrose and EDTA were compared for human immunodeficiency virus (HIV) infectivity on the day of collection or after 1 day of storage at room temperature. No significant differences between the anticoagulants were observed. Culture positivity was significantly associated with HIV RNA viral loads for both anticoagulants.
Fiscus
S A
SA
Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7140, USA. fiscussa@med.unc.edu
Chakraborty
H
H
Shepard
R
R
Goodman
M
M
D000925:Anticoagulants; D001777:Blood Coagulation; D001800:Blood Specimen Collection; D002478:Cells, Cultured; D019343:Citric Acid; D004492:Edetic Acid; D005947:Glucose / Q000031:analogs & derivatives*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D012367:RNA, Viral / Q000097:blood; D019562:Viral Load
D000925:Anticoagulants; D012367:RNA, Viral; C002113:acid citrate dextrose; D019343:Citric Acid; D004492:Edetic Acid; D005947:Glucose