Conformational switch in the decoding region of 16S rRNA during aminoacyl-tRNA selection on the ribosome.
Binding of aminoglycoside antibiotics to 16S ribosomal RNA induces a particular structure of the decoding center and increases the misincorporation of near-cognate amino acids. By kinetic analysis we show that this is due to stabilization of the near-cognate codon recognition complex and the acceleration of two rearrangements that limit the rate of amino acid incorporation. The same rearrangement steps are accelerated in the cognate coding situation. We suggest that cognate codon recognition, or near-cognate codon recognition augmented by aminoglycoside binding, promote the transition of 16S rRNA from a 'binding' to a 'productive' conformation that determines the fidelity of decoding.
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Institute of Molecular Biology, University of Witten/Herdecke, 58448 Witten, Germany.
Wintermeyer
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Rodnina
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D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001665:Binding Sites; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D007930:Leucine / Q000378:metabolism; D009690:Nucleic Acid Conformation; D010303:Paromomycin / Q000494:pharmacology; D010444:Peptide Elongation Factor Tu / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000187:drug effects / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S; D012346:RNA, Transfer, Amino Acyl; D012360:RNA, Transfer, Phe; D010303:Paromomycin; D006160:Guanosine Triphosphate; D010444:Peptide Elongation Factor Tu; D007930:Leucine