Selective removal of nitrogen from quinoline and petroleum by Pseudomonas ayucida IGTN9m.
Enrichment culture experiments employing soil and water samples obtained from petroleum-contaminated environments succeeded in the isolation of a pure culture possessing the ability to utilize quinoline as a sole nitrogen source but did not utilize quinoline as a carbon source. This culture was identified as Pseudomonas ayucida based on a partial 16S rRNA gene sequence, and the strain was given the designation IGTN9m. Examination of metabolites using thin-layer chromatography and gas chromatography-mass spectrometry suggests that P. ayucida IGTN9m converts quinoline to 2-quinolinone and subsequently to 8-hydroxycoumarin. Resting cells of P. ayucida IGTN9m were shown to be capable of selectively removing about 68% of quinoline from shale oil in a 16-h treatment time. These results suggest that P. ayucida IGTN9m may be useful in petroleum biorefining for the selective removal of organically bound nitrogen from petroleum.
Kilbane
J J
JJ
Institute of Gas Technology, Des Plaines, Illinois 60018, USA. kilbane@igt.org
Ranganathan
R
R
Cleveland
L
L
Kayser
K J
KJ
Ribiero
C
C
Linhares
M M
MM
D001673:Biodegradation, Environmental; D002855:Chromatography, Thin Layer; D004785:Environmental Pollutants / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009584:Nitrogen / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism*; D012988:Soil Microbiology; D014871:Water Microbiology
D004785:Environmental Pollutants; D010578:Petroleum; D011804:Quinolines; C037219:quinoline; D009584:Nitrogen