Gene-expression analysis at the single-cell level.
The manner in which a cell responds to and influences its environment is ultimately determined by the genes that it expresses. To fully understand and manipulate cellular function, identification of these expressed genes is essential. Techniques such as RT-PCR enable examination of gene expression at the tissue level. However, the study of complex heterogeneous tissue, such as the CNS or immune system, requires gene analysis to be performed at much higher resolution. In this article, the various methods that have been developed to enable RT-PCR to be performed at the level of the single cell are reviewed. In addition, how, when carried out in combination with techniques such as patch-clamp recording, single-cell gene-expression studies extend our understanding of biological systems is discussed.
Dixon
A K
AK
aDepartment of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge, UK CB2 1QJ. Alistair.dixon@wl.com
Richardson
P J
PJ
Pinnock
R D
RD
Lee
K
K
D002468:Cell Physiological Phenomena; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling; D006801:Humans; D009473:Neuronal Plasticity; D018408:Patch-Clamp Techniques; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*