Cardiac dendritic cells and acute myocarditis in the human heart.
Cardiac dendritic cells are considered to play an important role in the immunoresponse of the heart. However, it is unclear whether these cells occur in human myocarditis and whether they function in similar ways to those in rats. Cardiac samples were obtained from 22 autopsied patients with myocarditis, and compared with 20 age-and sex-matched controls. Formalin-fixed hearts were immunostained by the LSAB method. Cardiac dendritic cells were detectable even in the control hearts (1.5 cells/high power field (HPF)). In the acute phase of myocarditis, the number of cardiac dendritic cells increased up to 12.6 cells/HPF (p<0.001). In the subacute phase of myocarditis, T cells (36.6 cells/HPF) and HLA-DR+ cells (10.2 cells/HPF) continued to infiltrate the periphery of the inflammatory lesions, but they had no expression without inflammation. In this study, cardiac dendritic cells were reactive for HLA-DR, but negative for CD68, and were characteristically large monocytes with long, slender, dendritic processes. Accordingly, they were clearly distinguishable from macrophages. In the human heart, cardiac dendritic cells may be recruited in the acute phase of myocarditis, and seem to play an important role in the succeeding immunoresponse.
Yokoyama
H
H
Department of Internal Medicine and Cardiology, Kitasato University School of Medicine, Sagamihara, Japan. hiroyuki@med.kitasato-u.ac.jp
Kuwao
S
S
Kohno
K
K
Suzuki
K
K
Kameya
T
T
Izumi
T
T
D000208:Acute Disease; D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001344:Autopsy; D003713:Dendritic Cells / Q000166:cytology / Q000473:pathology*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology; D009205:Myocarditis / Q000473:pathology*; D009206:Myocardium / Q000166:cytology / Q000473:pathology*; D051381:Rats; D012016:Reference Values; D013601:T-Lymphocytes / Q000473:pathology; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D006684:HLA-DR Antigens; C067980:CD68 antigen, human; D000097382:CD68 Molecule