Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism.
The aim of the present study was to develop and validate a rapid assay for genotyping of CYP1B1 codon 432-polymorphism. The described method is a single tube assay and combines both rapid-cycle polymerase chain reaction (PCR) with real-time monitoring by amplification and generation of the melting profiles of an allele-specific fluorescent probe. With this method 300 samples were analysed from healthy, unrelated Germans. Genotype frequency determined for the mutated allele CYP1B1*2 was 0.40. The results show that genotyping of CYP1B1 codon 432-polymorphism with a real-time fluorescence PCR method is a rapid and reliable assay for the analysis of large numbers of samples.
Brüning
T
T
Institut für Arbeitsphysiologie an der Universität Dortmund, Germany.
Abel
J
J
Koch
B
B
Lorenzen
K
K
Harth
V
V
Donat
S
S
Sachinidis
A
A
Vetter
H
H
Bolt
H M
HM
Ko
Y
Y
D000483:Alleles; D001189:Aryl Hydrocarbon Hydroxylases; D003062:Codon / Q000235:genetics*; D065633:Cytochrome P-450 CYP1B1; D003577:Cytochrome P-450 Enzyme System / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*; D013050:Spectrometry, Fluorescence
D003062:Codon; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1