An advantage for use of isotope labeling and NMR chemical shifts to analyze the structure of four homologous IgG-binding domains of staphylococcal protein A.
Because of the complexity arising from the large molecular size and the amino acid sequence homologies of IgG-binding domains of Staphylococcal Protein A (SpA), we have introduced, a combination of stable isotope labeling and both qualitative and quantitative investigations of the structural dependence of the NMR chemical shifts for its structure analysis. In order to enable selective isotope labeling with high efficiency, a mutated low molecular weight Protein A (LPA; MWt = 27 kDa) which consists of E, D, A, B and 13 residues of the C-domain was used in this study. Amide proton chemical shifts, measured using uniformly 15N-labeled LPA and LPA labeled selectively with 15N-alanine, show that the turn between helices 1 and 2, and its tertiary interactions with helix 3, are very similar in all domains. This contradicts previous results obtained using independent structure calculations on isolated domains. The close similarity in NH and 15N chemical shifts of alanine residues in the interdomain linker suggests that the linker maintains a similar structure both in isolated domains and in the intact protein. We show that the high-field shifted methyl signal of Ala 48 is affected by the ring-current effect arising from Phe 30, and has a very similar helical environment in all four domains. Thus, helix 3 is present in all domains, as we previously reported [Kikuchi et al., J Biochem Biophys Method, 1999:38:203-208], even though it is not observed in the crystal structure [Deisenhofer J. Biochemistry 1981;20:2361-2370].
Kikuchi
J
J
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Japan.
Asakura
T
T
Hasuda
K
K
Ito
T
T
Ohwaku
K
K
Araki
H
H
Williamson
M P
MP
D000409:Alanine / Q000737:chemistry; D000577:Amides / Q000737:chemistry; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D002247:Carbon Isotopes; D007074:Immunoglobulin G / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D009587:Nitrogen Isotopes; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D011522:Protons; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D000577:Amides; D002247:Carbon Isotopes; D007074:Immunoglobulin G; D009587:Nitrogen Isotopes; D010446:Peptide Fragments; D011522:Protons; D013205:Staphylococcal Protein A; D000409:Alanine