title: An indexed genomic library for Paramecium complementation cloning.
abstract: Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.
authors.affiliation: Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
authors.forename: A M
authors.initials: AM
authors.lastname: Keller
authors.forename: J
authors.initials: J
authors.lastname: Cohen
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D016054:DNA, Protozoan / Q000032:analysis; D005816:Genetic Complementation Test; D015698:Genomic Library; D009693:Nucleic Acid Hybridization / Q000379:methods; D010247:Paramecium / Q000235:genetics* / Q000378:metabolism
chemical_list: D016054:DNA, Protozoan