Changes in myosin ATPase activity in skeletal muscles of rat during cold stress.
Three skeletal muscles viz., gastrocnemius, pectoralis and diaphragm from rats acclimated to a low temperature (4 +/- 1 degrees C; 16 hr daily; maximum for 8 weeks) exhibit an increased myosin ATPase activity. An analysis of native myosin from these muscles under non-dissociating conditions reveals two myosin isozymes instead of a single isozyme expressed in control muscles. Isoelectric focusing (IEF) coupled with two dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE) confirms an increased phosphorylation of myosin light chain 2 (MLC2) in muscles from cold acclimated rats.
Katoch
S S
SS
Department of Biosciences, Himachal Pradesh University, Shimla, India.
Soni
A
A
D000818:Animals; D003080:Cold Temperature; D018482:Muscle, Skeletal / Q000201:enzymology*; D009218:Myosins / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D013312:Stress, Physiological
D009218:Myosins