title: Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles.
abstract: Genome-wide transcript profiling was used to monitor signal transduction during yeast pheromone response. Genetic manipulations allowed analysis of changes in gene expression underlying pheromone signaling, cell cycle control, and polarized morphogenesis. A two-dimensional hierarchical clustered matrix, covering 383 of the most highly regulated genes, was constructed from 46 diverse experimental conditions. Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. Analysis of the profiles specified by two different MAPKs-Fus3p and Kss1p-revealed functional overlap of the filamentous growth and mating responses. Global transcript analysis reflects biological responses associated with the activation and perturbation of signal transduction pathways.
authors.affiliation: Rosetta Inpharmatics, 12040 115th Avenue Northeast, Kirkland, WA 98034, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Roberts
authors.forename: B
authors.initials: B
authors.lastname: Nelson
authors.forename: M J
authors.initials: MJ
authors.lastname: Marton
authors.forename: R
authors.initials: R
authors.lastname: Stoughton
authors.forename: M R
authors.initials: MR
authors.lastname: Meyer
authors.forename: H A
authors.initials: HA
authors.lastname: Bennett
authors.forename: Y D
authors.initials: YD
authors.lastname: He
authors.forename: H
authors.initials: H
authors.lastname: Dai
authors.forename: W L
authors.initials: WL
authors.lastname: Walker
authors.forename: T R
authors.initials: TR
authors.lastname: Hughes
authors.forename: M
authors.initials: M
authors.lastname: Tyers
authors.forename: C
authors.initials: C
authors.lastname: Boone
authors.forename: S H
authors.initials: SH
authors.lastname: Friend
mesh_terms: D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D016193:G1 Phase; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D008074:Lipoproteins / Q000494:pharmacology / Q000502:physiology; D020935:MAP Kinase Signaling System; D000072235:Mating Factor; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D005810:Multigene Family; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000494:pharmacology / Q000502:physiology; D010675:Pheromones; D011493:Protein Kinase C / Q000378:metabolism; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C081353:FAR1 protein, S cerevisiae; D005656:Fungal Proteins; D008074:Lipoproteins; C095063:MFA2 protein, S cerevisiae; D010455:Peptides; D010675:Pheromones; D012097:Repressor Proteins; C489241:STE12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000072235:Mating Factor; D011493:Protein Kinase C; C075580:FUS3 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases