title: CopA: An Escherichia coli Cu(I)-translocating P-type ATPase.
abstract: The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli. The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could be rescued by complementation by a plasmid carrying copA from E. coli or copB from Enterococcus hirae. Expression of copA was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing copA exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA.
authors.forename: C
authors.initials: C
authors.lastname: Rensing
authors.forename: B
authors.initials: B
authors.lastname: Fan
authors.forename: R
authors.initials: R
authors.lastname: Sharma
authors.forename: B
authors.initials: B
authors.lastname: Mitra
authors.forename: B P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000378:metabolism; D003300:Copper / Q000378:metabolism / Q000494:pharmacology; D003301:Copper Radioisotopes; D000073840:Copper-Transporting ATPases; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D016296:Mutagenesis; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012834:Silver / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; C071263:CopA protein, Bacteria; D003301:Copper Radioisotopes; D011993:Recombinant Fusion Proteins; D002104:Cadmium; D012834:Silver; D003300:Copper; D000251:Adenosine Triphosphatases; C109898:Zn(II)-translocating P-type ATPase; D000073840:Copper-Transporting ATPases; D015032:Zinc