Exocrine cell mitochondria of the rat pancreas after lead intoxication.
The aim of the study was to evaluate alterations in exocrine cell mitochondria of the rat pancreas after lead acetate intoxication. The experiment used 45 rats divided into 2 experimental groups receiving lead acetate to drink, of lead concentration 50 and 500 mg/dm3 (ppm), and a control group given tap water. The animals from the experimental group were decapitated after 2, 4, 6 and 8 weeks, 5 rats from the control group after 8 weeks of the experiment. Rats from experimental groups decapitated after 8 weeks had lead administration stopped after six weeks and then, for two weeks tap water was given. Pancreatic sections were examined with biochemical methods for the activity of cytochrome oxidase and succinic dehydrogenase. Ultrastructural and morphometric examinations were also performed. It was demonstrated that: a) exocrine cell mitochondria are particularly predisposed to lead effect, b) intoxication of rats with lower lead doses (50 ppm) causes reversible adaptative or compensatory changes in these organelles, c) intoxication of rats with higher lead doses (500 ppm) induces irreversible ultrastructural alterations in numerous mitochondria, including damage to inner and to outer mitochondrial membranes, d) structural changes in the mitochondria in the course of lead intoxication are the morphological expression of the impairment of metabolic processes, associated with the inhibited activity of the respiratory enzymes: succinic dehydrogenase and cytochrome oxidase.
Szynaka
B
B
Department of Pathological Anatomy, Medical University of Białystok, Poland.
Andrzejewska
A
A
Tomasiak
M
M
Augustynowicz
A
A
D000284:Administration, Oral; D000818:Animals; D004305:Dose-Response Relationship, Drug; D003576:Electron Transport Complex IV / Q000187:drug effects / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000648:ultrastructure*; D010179:Pancreas / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000187:drug effects / Q000378:metabolism
D007854:Lead; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV