title: Isolation and characterization of the mouse homolog of the preprodynorphin (Pdyn) gene.
abstract: We have isolated and sequenced the mouse preprodynorphin gene (Pdyn). The Pdyn gene can encode for six biologically active dynorphin peptides. The predicted mouse preprodynorphin has 90%, 67%, and 66% identity with the predicted rat, porcine, and human preprodynorphins, respectively. Using an RT-PCR technique, we show that the Pdyn gene starts being expressed at embryonic day 12.5, with a steep increase of expression by embryonic day 14.5; in the adult mouse it is expressed in the brain, but not in liver, heart, spleen, or kidney.
authors.affiliation: Unit on Molecular Genetics, NSB, Bethesda, MD, 20892, USA.
authors.forename: N
authors.initials: N
authors.lastname: Sharifi
authors.forename: M
authors.initials: M
authors.lastname: Ament
authors.forename: M B
authors.initials: MB
authors.lastname: Brennan
authors.forename: U
authors.initials: U
authors.lastname: Hochgeschwender
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004399:Dynorphins / Q000235:genetics*; D005091:Exons / Q000235:genetics; D005333:Fetus / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011498:Protein Precursors; C036448:pre-prodynorphin; D004399:Dynorphins