Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family.
Grb7 is a member of a family of molecular adapters which are able to contribute positively but also negatively to signal transduction and whose precise roles remain obscure. Rnd1 is a member of the Rho family, but, as opposed to usual GTPases, it is constitutively bound to GTP. We show here that Rnd1 and Grb7 interact, in two-hybrid assays, in vitro, and in pull-down experiments performed with SK-BR3, a breast cancer cell line that overexpresses Grb7. This interaction involves switch II loop of Rnd1, a region crucial for guanine nucleotide exchange in all GTPases, and a Grb7 SH2 domain, a region crucial for Grb7 interaction with several activated receptors. The contribution of the interaction between Rnd1 and Grb7 to their respective functions and properties is discussed.
Vayssière
B
B
Unité INSERM 248, Institut Curie, 26 rue d'Ulm, F-75248, Paris, France.
Zalcman
G
G
Mahé
Y
Y
Mirey
G
G
Ligensa
T
T
Weidner
K M
KM
Chardin
P
P
Camonis
J
J
D000906:Antibodies / Q000276:immunology; D001665:Binding Sites; D004926:Escherichia coli / Q000235:genetics; D051382:GRB7 Adaptor Protein; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000276:immunology / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014407:Tumor Cells, Cultured; D020798:Two-Hybrid System Techniques; D020741:rho GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018909:src Homology Domains
D000906:Antibodies; C495955:GRB7 protein, human; D011506:Proteins; C491772:RND1 protein, human; D011993:Recombinant Fusion Proteins; D051382:GRB7 Adaptor Protein; D019000:Phosphotyrosine; D020741:rho GTP-Binding Proteins