Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ-line transformation.
Germ-line transformation (vacuum infiltration) is frequently used to transform Arabidopsis thaliana using Agrobacterium tumefaciens. We have recently identified several Arabidopsis ecotypes and T-DNA-tagged mutants that are recalcitrant to Agrobacterium-mediated transformation of cut root segments. Some of these ecotypes and mutants are deficient in their ability to bind bacteria. Some are deficient in T-DNA integration. We report here that using a germ-line transformation protocol we transformed these ecotypes and mutants, including attachment- and integration-defective Arabidopsis plants, with a frequency similar to that of highly susceptible wild-type plants. However, we could not transform otherwise highly susceptible Arabidopsis plants by germ-line or root transformation using several vir and attachment-deficient Agrobacterium mutants. These results indicate that certain plant factors important for transformation may exist in germ-line tissue but may be lacking in some somatic cells.
Mysore
K S
KS
Purdue Genetics Program, Purdue University, West Lafayette, IN 47907-1392, USA.
Kumar
C T
CT
Gelvin
S B
SB
D017360:Arabidopsis / Q000235:genetics*; D018095:Germ-Line Mutation; D018517:Plant Roots / Q000382:microbiology*; D012231:Rhizobium / Q000502:physiology*; D014170:Transformation, Genetic