title: Cutting edge: role of B lymphocytes in protective immunity against Salmonella typhimurium infection.
abstract: Infection of mice with Salmonella typhimurium gives rise to a disease similar to human typhoid fever caused by S. typhi. Since S. typhimurium is a facultative intracellular bacterium, the requirement of B cells in the immune response against S. typhimurium is a longstanding matter of debate. By infecting mice on a susceptible background and deficient in B cells (Igmu-/- mice) with different strains of S. typhimurium, we could for the first time formally clarify the role of B cells in the response against S. typhimurium. Compared with Igmu+/+ mice, LD50 values in Igmu-/- mice were reduced during primary, and particularly secondary, oral infection with virulent S. typhimurium. After systemic infection, Igmu-/- mice cleared attenuated aroA- S. typhimurium, but vaccine-induced protection against systemic infection with virulent S. typhimurium involved both B cell-dependent and -independent effector mechanisms. Thus, B cell-mediated immunity plays a distinct role in control of S. typhimurium in susceptible mice.
authors.affiliation: Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany. mittruecker@mpiib-berlin.mpg.de
authors.forename: H W
authors.initials: HW
authors.lastname: Mittrücker
authors.forename: B
authors.initials: B
authors.lastname: Raupach
authors.forename: A
authors.initials: A
authors.lastname: Köhler
authors.forename: S H
authors.initials: SH
authors.lastname: Kaufmann
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D007275:Injections, Intravenous; D007928:Lethal Dose 50; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000276:immunology* / Q000472:pathogenicity; D014435:Typhoid Fever / Q000235:genetics / Q000276:immunology; D014436:Typhoid-Paratyphoid Vaccines / Q000008:administration & dosage / Q000276:immunology; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D014774:Virulence
chemical_list: D000907:Antibodies, Bacterial; D007148:Immunoglobulin mu-Chains; D014436:Typhoid-Paratyphoid Vaccines; D014613:Vaccines, Attenuated