title: [Loss of imprinting of insulin-like growth factor II gene in acute myeloid leukemia].
abstract: OBJECTIVE
  To investigate the status of insulin- like growth factor-2(IGF-2) gene imprinting in acute myeloid leukemia(AML).
  
  
  METHODS
  Selection of heterozygous cases for Apa I polymorphism within exon 9 of IGF-2 gene and further analysis of loss of imprinting(LOI) were done in 26 AML patients by polymerase chain reaction(PCR), reverse transcription PCR (RT-PCR) and restriction fragment length polymorphism(RFLP) technique.
  
  
  RESULTS
  Eleven cases of AML patients were identified as heterozygous for IGF-2(11/26); 8 heterozygous patients showed biallelic expression of IGF-2, indicating loss of IGF-2 imprinting.
  
  
  CONCLUSIONS
  The frequent LOI of IGF-2 may play a role in the development of acute myeloid leukemia.
authors.affiliation: Institute of Clinical Medical Sciences, Affiliated Hospital, Wannan Medical College, Wuhu, Anhui, 241001 P.R. China. Chenwork@163.net
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D018392:Genomic Imprinting; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged
chemical_list: D007335:Insulin-Like Growth Factor II