title: Stable microreentrant sources as a mechanism of atrial fibrillation in the isolated sheep heart.
abstract: BACKGROUND
  Atrial fibrillation (AF) has traditionally been described as aperiodic or random. Yet, ongoing sources of high-frequency periodic activity have recently been suggested to underlie AF in the sheep heart. Our objective was to use a combination of optical and bipolar electrode recordings to identify sites of periodic activity during AF and elucidate their mechanism.
  
  
  RESULTS
  AF was induced by rapid pacing in the presence of 0.1 to 0.5 micromol/L acetylcholine in 7 Langendorff-perfused sheep hearts. We used simultaneous optical mapping of the right and left atria (RA and LA) and frequency sampling of optical and bipolar electrode recordings (including a roving electrode) to identify sites having the highest dominant frequency (DF). Rotors were identified from optical recordings, and their rotation period, core area, and perimeter were measured. In all, 35 AF episodes were analyzed. Mean LA and RA DFs were 14.7+/-3.8 and 10.3+/-2.1 Hz, respectively. Spatiotemporal periodicity was seen in the LA during all episodes. In 5 of 7 experiments, a single site having periodic activity at the highest DF was localized. The highest DF was most often (80%) localized to the posterior LA, near or at the pulmonary vein ostium. Rotors (n=14) were localized on the LA. The mean core perimeter and area were 10.4+/-2.8 mm and 3.8+/-2.8 mm(2), respectively.
  
  
  CONCLUSIONS
  Frequency sampling allows rapid identification of discrete sites of high-frequency periodic activity during AF. Stable microreentrant sources are the most likely underlying mechanism of AF in this model.
authors.affiliation: Department of Pharmacology, SUNY Health Science Center, Syracuse, NY 13210, USA. mandapar@vax.cs.hscsyr.edu
authors.forename: R
authors.initials: R
authors.lastname: Mandapati
authors.forename: A
authors.initials: A
authors.lastname: Skanes
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: O
authors.initials: O
authors.lastname: Berenfeld
authors.forename: J
authors.initials: J
authors.lastname: Jalife
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000503:physiopathology*; D004594:Electrophysiology; D005583:Fourier Analysis; D006321:Heart / Q000503:physiopathology*; D066298:In Vitro Techniques; D055095:Optics and Photonics; D010507:Periodicity; D012756:Sheep