title: Presenilin-1 forms complexes with the cadherin/catenin cell-cell adhesion system and is recruited to intercellular and synaptic contacts.
abstract: In MDCK cells, presenilin-1 (PS1) accumulates at intercellular contacts where it colocalizes with components of the cadherin-based adherens junctions. PS1 fragments form complexes with E-cadherin, beta-catenin, and alpha-catenin, all components of adherens junctions. In confluent MDCK cells, PS1 forms complexes with cell surface E-cadherin; disruption of Ca(2+)-dependent cell-cell contacts reduces surface PS1 and the levels of PS1-E-cadherin complexes. PS1 overexpression in human kidney cells enhances cell-cell adhesion. Together, these data show that PS1 incorporates into the cadherin/catenin adhesion system and regulates cell-cell adhesion. PS1 concentrates at intercellular contacts in epithelial tissue; in brain, it forms complexes with both E- and N-cadherin and concentrates at synaptic adhesions. That PS1 is a constituent of the cadherin/catenin complex makes that complex a potential target for PS1 FAD mutations.
authors.affiliation: Department of Psychiatry, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: A
authors.initials: A
authors.lastname: Georgakopoulos
authors.forename: P
authors.initials: P
authors.lastname: Marambaud
authors.forename: S
authors.initials: S
authors.lastname: Efthimiopoulos
authors.forename: J
authors.initials: J
authors.lastname: Shioi
authors.forename: W
authors.initials: W
authors.lastname: Cui
authors.forename: H C
authors.initials: HC
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Schütte
authors.forename: R
authors.initials: R
authors.lastname: Gordon
authors.forename: G R
authors.initials: GR
authors.lastname: Holstein
authors.forename: G
authors.initials: G
authors.lastname: Martinelli
authors.forename: P
authors.initials: P
authors.lastname: Mehta
authors.forename: V L
authors.initials: VL
authors.lastname: Friedrich
authors.forename: N K
authors.initials: NK
authors.lastname: Robakis
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004285:Dogs; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D053764:Presenilin-1; D011817:Rabbits; D013569:Synapses / Q000378:metabolism*
chemical_list: D015820:Cadherins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1