title: Effects of intestinal bacteria on the development of colonic neoplasm II. Changes in the immunological environment.
abstract: To study the effects of intestinal bacteria on the development of colonic neoplasm, we have established gnotobiotic mice with a single species of intestinal bacteria. In the previous study, the incidence of colonic adenoma induced with 1,2-dimethylhydrazine (DMH) in the gnotobiotic mice with Lactobacillus acidophilus, gnotobiotic mice with Escherichia coli and germ-free mice were 30, 50 and 74%, respectively. In this study, 7-week-old mice in each group were sacrificed without the administration of DMH to examine the constituents of immuno-competent cells in various mouse organs using flow cytometry. In the gnotobiotic mice, CD3 intermediate interleukin (IL)-2Rbeta positive cells were observed predominantly in the liver. In the gnotobiotic mice with L. acidophilus, Mac-1 positive Gr-1 positive cells were observed predominantly in the colonic lamina propria. The activation of extrathymic T cells in the liver and granulocytes in the colonic mucosa may be related to anti-neoplastic effects of L. acidophilus in this experimental model.
authors.affiliation: Department of Surgery, Centre for Molecular Medicine, Jichi Medical School, Tochigi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Horie
authors.forename: K
authors.initials: K
authors.lastname: Kanazawa
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: A
authors.initials: A
authors.lastname: Fujimura
authors.forename: S
authors.initials: S
authors.lastname: Yamagiwa
authors.forename: T
authors.initials: T
authors.lastname: Abo
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000494:pharmacology; D000236:Adenoma / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control; D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D002273:Carcinogens / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000276:immunology* / Q000382:microbiology* / Q000517:prevention & control; D004926:Escherichia coli / Q000502:physiology; D005260:Female; D005856:Germ-Free Life; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology; D007422:Intestines / Q000276:immunology* / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000502:physiology; D008099:Liver / Q000201:enzymology / Q000276:immunology; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D017252:CD3 Complex; D002273:Carcinogens; D007376:Interleukin-2; D019813:1,2-Dimethylhydrazine