Noncovalent adducts of poly(ethylene glycols) with proteins.
A new method of preparation of noncovalent complexes between poly(ethylene glycol) (PEG) and proteins (alpha-chymotrypsin (ChT), lysozyme, bovine serum albumine) under high pressure has been developed. The involvement of polymer in the complexes was proved using (3)H-labeled PEG. The composition of the complexes (the number of polymer chains per one ChT molecule) depends on the molecular mass of PEG and decreases with the increase in molecular mass from 300 to 4000, whereas the portion of the protein (wt %) in complexes does not depend on the molecular mass of incorporated PEG and corresponds to approximately 70 wt %. The kinetic constants for enzymatic hydrolysis of N-benzoyl-L-tyrosine ethyl ester and azocasein catalyzed by the PEG-ChT complexes are identical with the corresponding values for the native ChT. According to the data obtained by the method of circular dichroism, the enzyme in the complexes fully retains its secondary structure. The steric availability of PEG polymer chains in the complexes was evaluated by their complexation with alpha-cyclodextrin (CyD) or polymer derivatives of beta-CyD modified with PEG (PEG-beta-CyD). In contrast to free PEG, only part of PEG polymer chains ( approximately 10%) interact with alpha-CyD. Thus, the complexation of PEG with ChT proceeds by means of multipoint interaction with surface groups of the protein globule located far from the active site and results in the sufficient decrease in the availability of polymer chains. The complexes between PEG chains in PEG-protein adducts and PEG-beta-CyD may be considered as a novel type of dendritic structures.
Topchieva
I N
IN
Lomonosov Moscow State University, Department of Chemistry, Leninskii Gory, 119899, Moscow, Russia.
Sorokina
E M
EM
Efremova
N V
NV
Ksenofontov
A L
AL
Kurganov
B I
BI
D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism; D003505:Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry; D004795:Enzyme Stability; D006868:Hydrolysis; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry; D011312:Pressure; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012996:Solutions; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D003505:Cyclodextrins; D011506:Proteins; D012996:Solutions; D012710:Serum Albumin, Bovine; C006716:benzoyltyrosine ethyl ester; D011092:Polyethylene Glycols; D014443:Tyrosine; D009113:Muramidase; D002918:Chymotrypsin; C117608:alpha-chymotrypsin