Future molecular approaches to the diagnosis and treatment of glomerular disease.
Current diagnoses and treatment decisions for renal disease are made based upon a combination of clinical and pathological determinations. With the advances in both biochemical and molecular biological techniques, identifying the underlying biochemical and genetic changes that may have initiated and/or contributed to the disease is possible. We describe here technologies that may lead to significant changes in renal disease diagnosis, characterization, treatment, and potentially prevention. For example, differential display techniques and DNA gene chip arrays show the changes in mRNA expression patterns and can potentially identify previously unknown genes and reveal new roles for previously known genes in renal disease. The generation of the single nucleotide polymorphisms (SNP) genomic map will facilitate genetic screening that may identify a gene or combination of genes that produce enhanced disease susceptibility. Combining genomic analysis with epidemiological studies may identify environmental factors that contribute to renal disease onset in genetically susceptible individuals. A number of novel therapies are already on the horizon. These include reagents that abrogate the function of specific cytokines, chemokines, and effector cells. With the list of renal disease genes in hand, their role in renal physiology and pathophysiology can be determined, which should lead to the discovery of pharmacological intervention directed at those genes and their products that play a role in the pathogenesis of renal disease.
Alcorta
D A
DA
Department of Medicine, University of North Carolina, Chapel Hill 27599-7155, USA. David_Alcorta@med.unc.edu
Prakash
K
K
Waga
I
I
Sasai
H
H
Munger
W
W
Jennette
J C
JC
Falk
R J
RJ
D001709:Biotechnology / Q000379:methods*; D005820:Genetic Testing; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D007678:Kidney Glomerulus; D010597:Pharmacogenetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA