Antihepatotoxic activity of bergenin, the major constituent of Mallotus japonicus, on carbon tetrachloride-intoxicated hepatocytes.
To determine the antihepatotoxic activity of bergenin from Mallotus japonicus, carbon tetrachloride (CCl4)-induced cytotoxicity in primary cultured rat hepatocytes has been adopted as an assay system. Bergenin significantly reduced the activities of glutamic pyruvic transaminase and sorbitol dehydrogenase released from the CCl4-intoxicated hepatocytes. The antihepatotoxicity of bergenin was also evidenced by elevating the activities of glutathione S-transferase and glutathione reductase, and content of glutathione in the CCl4-intoxicated hepatocytes. From these results, it is assumed that bergenin exerted antihepatotoxicity against CCl4-induced cytotoxicity through glutathione-mediated detoxification as well as free radical suppressing activity.
Kim
H S
HS
College of Pharmacy, Chungbuk National University, Cheongju, South Korea. hskim@trut.chungbuk.ac.kr
Lim
H K
HK
Chung
M W
MW
Kim
Y C
YC
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002252:Carbon Tetrachloride Poisoning / Q000150:complications*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D019665:Euphorbiaceae / Q000737:chemistry*; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D051381:Rats
D001578:Benzopyrans; D007064:L-Iditol 2-Dehydrogenase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D000410:Alanine Transaminase; C006741:bergenin