title: Characterization of a Casuarina glauca nodule-specific subtilisin-like protease gene, a homolog of Alnus glutinosa ag12.
abstract: In search of plant genes expressed during early interactions between Casuarina glauca and Frankia, we have isolated and characterized a C. glauca gene that has strong homology to subtilisin-like protease gene families of several plants including the actinorhizal nodulin gene ag12 of another actinorhizal plant, Alnus glutinosa. Based on the expression pattern of cg12 in the course of nodule development, it represents an early actinorhizal nodulin gene. Our results suggest that subtilisin-like proteases may be a common element in the process of infection of plant cells by Frankia in both Betulaceae (Alnus glutinosa) and Casuarinaceae (Casuarina glauca) symbioses.
authors.affiliation: Physiologie Cellulaire et Moléculaire des Arbres, Laboratoire GeneTrop, IRD, Montpellier, France.
authors.forename: L
authors.initials: L
authors.lastname: Laplaze
authors.forename: A
authors.initials: A
authors.lastname: Ribeiro
authors.forename: C
authors.initials: C
authors.lastname: Franche
authors.forename: E
authors.initials: E
authors.lastname: Duhoux
authors.forename: F
authors.initials: F
authors.lastname: Auguy
authors.forename: D
authors.initials: D
authors.lastname: Bogusz
authors.forename: K
authors.initials: K
authors.lastname: Pawlowski
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology* / Q000382:microbiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013381:Subtilisins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013559:Symbiosis / Q000235:genetics; D014197:Trees / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
chemical_list: D010940:Plant Proteins; D013381:Subtilisins; C095312:ag12 protein, Alnus glutinosa