Localization of bcl-2 mRNA in the rabbit central nervous system.
In this study, we demonstrated bcl-2 expression in the rabbit brain using in situ hybridization and compared the results with the studies done on the other animals. Although the bcl-2 expressions of the rabbit were generally similar to those of the other animals, some differences were also found; the cells in molecular and Purkinje cell layers of the cerebellum, which were bcl-2 negative in the others, showed intense bcl-2 positive signals, while the ependymal cells, arachnoid villi and granular layer, which were positive in the other animals, were not well stained with bcl-2 riboprobes in the rabbit brain. Our findings clearly showed the similarities and differences of rabbit bcl-2 mRNA as compared with the other species for the first time, and provided the basis of further study on the properties of bcl-2 in this species.
Shin
D H
DH
Department of Anatomy, Dankook University College of Medicine, Chonan, South Korea.
Lee
H Y
HY
Lee
K H
KH
Kim
H J
HJ
Lee
W J
WJ
Hwang
D H
DH
Baik
S H
SH
Cho
S S
SS
D000818:Animals; D001923:Brain Chemistry; D019254:Genes, bcl-2; D017403:In Situ Hybridization; D009419:Nerve Tissue Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D011817:Rabbits / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
D009419:Nerve Tissue Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger