Acute ibogaine injection induces expression of the immediate early genes, egr-1 and c-fos, in mouse brain.
The aim of the present study was to evaluate if an acute injection of ibogaine (IBO) induces immediate early gene expression in different regions of mouse brain. Adult male C57 mice received a single injection of IBO and were perfused transcardially with 1% paraformaldehyde 30 min after the drug administration. A single injection of IBO produced a significant increase of egr-1 messenger RNA induction in nucleus accumbens (NAc), caudate-putamen (CPu), frontal cortex (FCx), septum, dentate gyrus (DG) and CA3 region of hippocampus, whereas c-fos gene was induced in CPu, FCx, DG, septum and CA1 region of hippocampus. This gene expression may be due, in part, to the stimulant properties of IBO, as we found with other psychostimulants.
Ali
S F
SF
Neurochemistry Laboratory, Division of Neurotoxicology, HFT-132, National Center Toxicological Research, FDA, 3900 NCTR Road, Jefferson, AR 72079-9502, USA. sali@nctr.fda.gov
Thiriet
N
N
Zwiller
J
J
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002421:Caudate Nucleus / Q000187:drug effects / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism; D051766:Early Growth Response Protein 1; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017781:Genes, Immediate-Early / Q000235:genetics*; D016762:Genes, fos / Q000235:genetics*; D006213:Hallucinogens / Q000008:administration & dosage*; D007050:Ibogaine / Q000008:administration & dosage*; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism; D011699:Putamen / Q000187:drug effects / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020665:Septum of Brain / Q000187:drug effects / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D006213:Hallucinogens; D017874:Immediate-Early Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D014157:Transcription Factors; D007050:Ibogaine