Comparative evaluation of sensitivity of RNA-polyacrylamide gel electrophoresis and dot immunobinding assay for detection of bluetongue virus in cell culture.
Bluetongue virus serotype 1 (Avikanagar isolate) was grown in BHK-21 cell line and titrated. The titre of the virus in BHK-21 cell line was 10(6) TCID50/ml. RNA-polyacrylamide gel electrophoresis (RNA-PAGE) and dot immunobinding assay (DIA) were performed on 10-fold serial dilutions of the sonicated cell culture material. The results indicated that the minimum limit of detection of the virus by RNA-PAGE and DIA was 10(5) TCID50/ml.
Prasad
G
G
Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, India.
Minakshi
D000818:Animals; D001820:Bluetongue virus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000706:statistics & numerical data; D015151:Immunoblotting / Q000706:statistics & numerical data; D012367:RNA, Viral / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D012367:RNA, Viral