Activation of mitogen-activated protein kinases in gerbil hippocampus with ischemic tolerance induced by 3-nitropropionic acid.
The present study investigated the activation of c-Jun NH2-terminal kinases (JNK), p38 mitogen-activated protein kinases (p38) and extracellular signal-regulated kinases (ERK) in the gerbil hippocampus by immunohistochemistry to clarify the role of these kinases in ischemic tolerance induced by3-NP. Intraperitoneal administration of 3-NP (3 or 10 mg/kg) caused the activation of JNK in CA1 subfield, which induced tolerance to subsequent ischemia and prevented delayed neuronal death (DND). As concerns p38 and ERK, no activation was induced by intoxication of 3-NP. Our results show the activation of JNK following chemical preconditioning with low dose of 3-NP is closely related to the acquisition of resistance to DND.
Sugino
T
T
Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Japan. ssug@kuhp.kyoto-u.ac.jp
Nozaki
K
K
Hashimoto
N
N
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000201:enzymology; D002470:Cell Survival; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005849:Gerbillinae; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology*; D007124:Immunoenzyme Techniques; D019194:Ischemic Preconditioning; D048031:JNK Mitogen-Activated Protein Kinases; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000187:drug effects / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000187:drug effects / Q000378:metabolism*; D009574:Nitro Compounds; D011422:Propionates / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases
D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D009574:Nitro Compounds; D011422:Propionates; D048031:JNK Mitogen-Activated Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C015392:3-nitropropionic acid