Isolation of Angiostrongylus costaricensis first-stage larvae from rodent feces on a Percoll gradient.
Several density gradients were tested for the isolation of parasitic nematode, Angiostrongylus costaricensis, first-stage larvae from rodent feces. With a 45/72% Percoll gradient, 83-99% (89.56+/-6.57%) of the larvae were recovered in a clean preparation.
Graeff-Teixeira
C
C
Laboratórios de Biologia Parasitária e Parasitologia Molecular, Faculdade de Biociências e Instituto de Pesquisas Biomédicas, PUCRS, Porto Alegre, Brazil.
Geiger
S
S
Walderich
B
B
Hoffmann
W
W
Abrahams
E
E
Schulz-Key
H
H
D000801:Angiostrongylus / Q000302:isolation & purification*; D000818:Animals; D002499:Centrifugation, Density Gradient; D003102:Colloids; D005243:Feces / Q000469:parasitology*; D007814:Larva; D051379:Mice; D008809:Mice, Inbred C3H; D011205:Povidone; D012376:Rodent Diseases / Q000469:parasitology*; D012822:Silicon Dioxide; D017206:Strongylida Infections / Q000469:parasitology / Q000662:veterinary*
D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone