Animal products and K-ras codon 12 and 13 mutations in colon carcinomas.
K-ras gene mutations (codons 12 and 13) were determined by PCR-based mutant allele-specific amplification (MASA) in tumour tissue of 185 colon cancer patients: 36% harboured mutations, of which 82% were located in codon 12. High intakes of animal protein, calcium and poultry were differently associated with codon 12 and 13 mutations: odds ratios (OR) and 95% confidence intervals (95% CI) for codon 12 versus codon 13 were 9.0 (2.0-42), 4.1 (1.4-12) and 15 (1.4-160), respectively. In case-control comparisons, high intakes of animal protein and calcium were positively associated with colon tumours harbouring codon 12 mutations [for animal protein per 17 g, OR (95% CI) = 1.5 (1.0-2.1); for calcium per 459 mg, 1.2 (0.9-1.6)], while inverse associations were observed for tumours with K-ras mutations in codon 13 [for animal protein 0.4 (0.2-1.0); for calcium 0.6 (0. 3-1.2)]. Transition and transversion mutations were not differently associated with these dietary factors. These data suggest a different dietary aetiology of colon tumours harbouring K-ras codon 12 and 13 mutations.
Kampman
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E
Division of Human Nutrition and Epidemiology, Wageningen University, Dreijenlaan 1 (bode 154), 6703 HA Wageningen, the Netherlands. ellen.kampman@staff.nutepi.wau.nl
Voskuil
D W
DW
van Kraats
A A
AA
Balder
H F
HF
van Muijen
G N
GN
Goldbohm
R A
RA
van't Veer
P
P
D000328:Adult; D000368:Aged; D000818:Animals; D002136:Calcium, Dietary / Q000009:adverse effects*; D002277:Carcinoma / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D003062:Codon / Q000235:genetics*; D003110:Colonic Neoplasms / Q000209:etiology / Q000235:genetics*; D018736:DNA Adducts; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D003611:Dairy Products; D004041:Dietary Fats; D004044:Dietary Proteins / Q000009:adverse effects*; D005247:Feeding Behavior; D005260:Female; D005399:Fishes; D011905:Genes, ras; D006801:Humans; D008297:Male; D008460:Meat / Q000009:adverse effects*; D008875:Middle Aged; D009154:Mutation; D009426:Netherlands; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011200:Poultry; D011795:Surveys and Questionnaires
D002136:Calcium, Dietary; D003062:Codon; D018736:DNA Adducts; D004273:DNA, Neoplasm; D004041:Dietary Fats; D004044:Dietary Proteins