A role for myosin-I in actin assembly through interactions with Vrp1p, Bee1p, and the Arp2/3 complex.
Type I myosins are highly conserved actin-based molecular motors that localize to the actin-rich cortex and participate in motility functions such as endocytosis, polarized morphogenesis, and cell migration. The COOH-terminal tail of yeast myosin-I proteins, Myo3p and Myo5p, contains an Src homology domain 3 (SH3) followed by an acidic domain. The myosin-I SH3 domain interacted with both Bee1p and Vrp1p, yeast homologues of human WASP and WIP, adapter proteins that link actin assembly and signaling molecules. The myosin-I acidic domain interacted with Arp2/3 complex subunits, Arc40p and Arc19p, and showed both sequence similarity and genetic redundancy with the COOH-terminal acidic domain of Bee1p (Las17p), which controls Arp2/3-mediated actin nucleation. These findings suggest that myosin-I proteins may participate in a diverse set of motility functions through a role in actin assembly.
Evangelista
M
M
Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
Klebl
B M
BM
Tong
A H
AH
Webb
B A
BA
Leeuw
T
T
Leberer
E
E
Whiteway
M
M
Thomas
D Y
DY
Boone
C
C
D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins / Q000378:metabolism / Q000502:physiology*; D000595:Amino Acid Sequence; D002465:Cell Movement / Q000502:physiology; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins / Q000378:metabolism; D008024:Ligands; D008840:Microfilament Proteins / Q000378:metabolism; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology; D018995:Myosin Heavy Chains / Q000378:metabolism; D024461:Myosin Type I; D009218:Myosins / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D020798:Two-Hybrid System Techniques; D051316:Wiskott-Aldrich Syndrome Protein
D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins; D003598:Cytoskeletal Proteins; D005656:Fungal Proteins; C480361:LAS17 protein, S cerevisiae; D008024:Ligands; C491722:MYO3 protein, S cerevisiae; C100412:MYO5 protein, S cerevisiae; D008840:Microfilament Proteins; D020409:Molecular Motor Proteins; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; C090350:VRP1 protein, S cerevisiae; D051316:Wiskott-Aldrich Syndrome Protein; D024461:Myosin Type I; D018995:Myosin Heavy Chains; D009218:Myosins