[Molecular cloning of cDNAs coding Sarcophaga diptericin family].
We purified an antibacterial protein named sarcotoxin III from the hemolymph of injured larvae of Sarcophaga peregrina (Baba K., et al., J. Biochem., 102, 69-74 (1987)). In this report we determined the partial amino acid sequence of sarcotoxin III and tried to conduct the cloning of sarcotoxin III cDNA. As a result we obtained two clones coding a protein similar to sarcotoxin III in sequence. Their primary structures are highly homologous to N-terminal sequences of an antibacterial protein from Phormia, diptericin, and another 8 kD antibacterial protein purified from Sarcophaga peregrina. These results suggested that sarcotoxin III is one of the proteins forming a diptericin family in Sarcophaga peregrina as well as newly obtained clone-coded products and 8 kD protein.
Baba
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Kubo
T
T
Natori
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004175:Diptera / Q000737:chemistry*; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011679:Pupa / Q000737:chemistry
D000890:Anti-Infective Agents; D019476:Insect Proteins; C054070:sarcotoxin III protein, Sarcophaga peregrina; D004247:DNA