title: Neuroprotective effects of an antioxidant in cortical cerebral ischemia: prevention of early reduction of the apurinic/apyrimidinic endonuclease DNA repair enzyme.
abstract: We examined the effects of the free radical scavenger, 21-aminosteroid, on apurinic/apyrimidinic endonuclease (APE/Ref-1) protein expression and subsequent infarction volume after photothrombotic cortical cerebral ischemia in mice. Immunohistochemistry and Western blot analysis showed a significant reduction in APE/Ref-1 expression 6 and 24 h after ischemia in untreated animals, whereas in drug-treated animals the reduction was much less at the same time points. The administration of 21-aminosteroid significantly decreased subsequent infarction volume 3 days after ischemia. These data suggest that 21-aminosteroid prevents the early decrease of APE/Ref-1 expression, thereby reducing cortical infarction after photothrombotic cerebral ischemia.
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Chang
authors.forename: M
authors.initials: M
authors.lastname: Fujimura
authors.forename: Y
authors.initials: Y
authors.lastname: Morita-Fujimura
authors.forename: G W
authors.initials: GW
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Huang
authors.forename: H S
authors.initials: HS
authors.lastname: Wu
authors.forename: M
authors.initials: M
authors.lastname: Kawase
authors.forename: J C
authors.initials: JC
authors.lastname: Copin
authors.forename: P H
authors.initials: PH
authors.lastname: Chan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D019757:Carbon-Oxygen Lyases / Q000187:drug effects* / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects* / Q000503:physiopathology; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011281:Pregnatrienes / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D011281:Pregnatrienes; C074965:U 74389F; D019757:Carbon-Oxygen Lyases; C486216:Apex1 protein, mouse; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase