Visualisation of phenotypically mixed HIV-1 and HTLV-I virus particles by electron microscopy.
Virions produced after HIV-1 infection of HTLV-I transformed cells have an expanded tropism that has been attributed to the presence of HTLV-I glycoproteins in the envelope. This report now directly identifies these phenotypically mixed virions by immunogold labelling electron microscopy. Furthermore we estimate there are 2% of these in cell-free supernatant, which represents up to 1 x 10(7) particles/ml from an in vitro infection. HTLV-1 envelope labelling was localised to a single region, suggesting a defined event in packaging of foreign envelope proteins into HIV-1 virus particles.
Lawson
V A
VA
AIDS Cellular Biology Unit, National Centre for HIV Virology Research, Macfarlane Burnet Centre for Medical Research, Fairfield, Australia. lawson@burnet.edu.au
Lee
J Y
JY
Doultree
J C
JC
Marshall
J A
JA
McPhee
D A
DA
D000914:Antibodies, Viral / Q000032:analysis; D002461:Cell Line, Transformed / Q000821:virology; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure*; D015368:Human T-lymphotropic virus 1 / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron / Q000379:methods; D010641:Phenotype; D011233:Precipitin Tests; D012191:Retroviridae Proteins / Q000032:analysis / Q000276:immunology; D014771:Virion / Q000737:chemistry / Q000276:immunology
D000914:Antibodies, Viral; D012191:Retroviridae Proteins