title: Efficacy of N-acetylcysteine and all-trans retinoic acid in restoring in vitro effective hemopoiesis in myelodysplastic syndromes.
abstract: We evaluated the in vitro effect on clonogenic potential (CFU-GM) and apoptosis in myelodysplastic syndromes (MDS) progenitors of an anti-oxidant (N-acetylcysteine, NAC) and/or a differentiating (all-trans retinoic acid, ATRA) agent. NAC significantly reduced apoptosis, both NAC and ATRA induced an increase in CFU-GM, but NAC seemed to be particularly effective in the high risk (HR) MDS. NAC + ATRA conferred a significant advantage in terms of CFU-GM with respect to NAC and ATRA alone. Tumor Necrosis Factor-alpha (TNF-alpha) levels decreased after incubation with NAC in the MDS samples. This study shows that ineffective hemopoiesis in MDS could benefit from both NAC and ATRA, suggesting that anti-oxidant treatment may play a role in guaranteeing MDS cell survival, predisposing them towards differentiation.
authors.affiliation: Servizio Autonomo di Ematologia Diagnostica, Ospedale Maggiore Policlinico IRCCS, Milan, Italy. cortelez@polic.cilea.it
authors.forename: A
authors.initials: A
authors.lastname: Cortelezzi
authors.forename: C
authors.initials: C
authors.lastname: Cattaneo
authors.forename: B
authors.initials: B
authors.lastname: Sarina
authors.forename: S
authors.initials: S
authors.lastname: Cristiani
authors.forename: M
authors.initials: M
authors.lastname: Pomati
authors.forename: I
authors.initials: I
authors.lastname: Silvestris
authors.forename: M
authors.initials: M
authors.lastname: Motta
authors.forename: A
authors.initials: A
authors.lastname: Ibatici
authors.forename: G
authors.initials: G
authors.lastname: Gornati
authors.forename: A D
authors.initials: AD
authors.lastname: Volpe
authors.forename: A T
authors.initials: AT
authors.lastname: Maiolo
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D014409:Tumor Necrosis Factor-alpha; D014212:Tretinoin; D000111:Acetylcysteine