[RAPD analysis of genetic diversity and phylogenetic relationship of the Taihu pigs].
The genetic diversity and phylogenetic relationship of the Taihu pigs were studied using RAPD technique. A total of 57 individuals were used, and the phylogenetic tree was reconstructed using UPGMA method based on 198 RAPD markers which were amplified with 13 decamer primers. The results indicate as the following: (1) The similarity indexes within each strain and between the strains of the Taihu pigs are all high. The average genetic distance between the strains is 0.052. These results indicate that the Taihu pigs exhibit relatively low level of genetic variation and poor genetic diversity; (2) The Hongdenglong pig is also a strain of the Taihu pigs; (3) The Taihu pigs have close phylogenetic relationship with the Chinese wild boar occurred in the Taihu Lake area; (4) Two lineages diverged in the early stage during the course of the formation of the Taihu pigs. One of the lineage gave rise to the strains of Mizhu, Erhualian, Jiaxinhei and Hongdenglong, another lineage gave rise to the strains of Shawutou, Meishan, Fengjing and Hengjing. The strategy for genetic diversity conservation of the Taihu pigs was discussed.
Chang
Q
Q
Biodiversity and Molecular Evolution Research Laboratory, Nanjing Normal University.
Zhou
K Y
KY
Wang
Y Q
YQ
Zhang
Z K
ZK
Cao
X
X
D000818:Animals; D014644:Genetic Variation; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D013552:Swine / Q000235:genetics*