Inhibition by antisense oligonucleotides of plasma membrane Ca(2+) ATPase in vascular endothelial cells.
Antisense oligodeoxynucleotides were used to knock down plasma membrane Ca(2+) ATPase, and the role of plasma membrane Ca(2+) ATPase was investigated in human aortic endothelial (HAE) cells. The peak of thapsigargin-evoked intracellular Ca(2+) concentration ([Ca(2+)](i)) was higher in antisense-treated than in untreated cells, but the declining time course was unaffected by the antisense treatment. The declining time was prolonged in both antisense-treated and untreated cells by reducing external Na(+), but the prolongation was more marked in the antisense-treated cells. These results provide the evidence of a functional role of plasma membrane Ca(2+) ATPase, although other mechanisms including Na(+)/Ca(2+) exchange may play the primary role in regulating [Ca(2+)](i).
Nakao
M
M
Department of Pharmacology, Akita University School of Medicine, 1-1-1 Hondoh, Akita, Japan.
Furukawa
K
K
Satoh
E
E
Ono
K
K
Iijima
T
T
D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000201:enzymology*; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism; D019284:Thapsigargin / Q000494:pharmacology
D016376:Oligonucleotides, Antisense; D019284:Thapsigargin; D012964:Sodium; D000252:Calcium-Transporting ATPases; D002118:Calcium