Cloning and sequencing of an apparently recombinant promoter for napin gene from Brassica campestris genomic library and its evolutionary significance.
From a genomic library of Brassica campestris (brown sarson cv. B54), we have cloned and sequenced about 2 kb of upstream regulatory region from one of the 2S albumin-coding gene family. The sequence has several seed-specific promoter motifs. A sequence alignment of the 5' flanking regions of the available Brassica 2S storage protein genes showed that our sequence is a double crossover recombinant product of the two members of the napin gene family. A possible explanation of this fact is that Brassica species evolved through gene duplication and recombination from a common ancestor with fewer number of chromosomes and genes.
Bhattacharyya
S
S
Department of Biochemistry, Bose Institute, Calcutta, West Bengal, India.
Mandal
R K
RK
D055516:2S Albumins, Plant; D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
D055516:2S Albumins, Plant; D018076:DNA, Complementary; D010940:Plant Proteins; C050363:napin protein, Brassica napus