Effects of terminal deletions in C5 protein on promoting RNase P catalysis.
Deletion derivatives of C5 protein, the protein cofactor of Escherichia coli RNase P, were constructed as soluble MBP (maltose-binding protein) fusion proteins to assess the deletion effects on promoting RNase P catalysis and on binding to M1 RNA, the catalytic subunit of the enzyme. The C5 protein, with large terminal deletions, retained its promoting activity of RNase P catalysis under protein excess conditions in vitro. Some deletion derivatives complemented the temperature sensitive phenotype of E. coli A49 cells carrying the rnpA49 mutation. This ability also suggests that part of the C5 protein is enough to produce the catalytic activity of RNase P in vivo. Both the central conserved region, called the RNR motif, and the C-terminal region are essential for the binding of C5 protein to M1 RNA. Meanwhile, the N-terminal region contributes to promoting RNase P catalysis in ways other than binding to M1 RNA.
Kim
M
M
Department of Chemistry, Korea Advanced Institute of Science and Technology, Taejon, 305-701, Korea.
Hyun Park
B
B
Lee
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002384:Catalysis; D020134:Catalytic Domain / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004722:Endoribonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010641:Phenotype; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D016337:RNA, Catalytic / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D043262:Ribonuclease P; D017384:Sequence Deletion
D001426:Bacterial Proteins; D017931:DNA Primers; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D012360:RNA, Transfer, Phe; D011993:Recombinant Fusion Proteins; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli