In vitro percutaneous absorption of fusidic acid and betamethasone 17-valerate across canine skin.
The penetration of betamethasone 17-valerate and fusidic acid through dog skin was measured in vitro. In order to detect the small amounts of diffused compounds, a liquid chromatography-mass spectrometric method was developed with sensitivity limits for both compounds of 5 ng/ml. After application to the skin surface of a topical gel preparation containing these compounds at the anticipated therapeutic dose rate, about 10 per cent of the betamethasone 17-valerate penetrated the skin over a 24-hour period, with no significant metabolism of the ester. About 1.3 per cent of the applied dose of fusidic acid was similarly recovered. The results demonstrated rapid penetration of both compounds through the epidermis.
Degim
I T
IT
Welsh School of Pharmacy, Cardiff University, Cardiff.
Hadgraft
J
J
Houghton
E
E
Teale
P
P
D000279:Administration, Cutaneous; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001624:Betamethasone Valerate / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid; D004285:Dogs / Q000378:metabolism*; D005672:Fusidic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005938:Glucocorticoids; D013058:Mass Spectrometry; D012680:Sensitivity and Specificity; D012869:Skin Absorption
D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D005672:Fusidic Acid; D001624:Betamethasone Valerate