title: Aligned microcontact printing of micrometer-scale poly-L-lysine structures for controlled growth of cultured neurons on planar microelectrode arrays.
abstract: We describe a method for producing high-resolution chemical patterns on surfaces to control the attachment and growth of cultured neurons. Microcontact printing has been extended to allow the printing of micron-scale protein lines aligned to an underlying pattern of planar microelectrodes. Poly-L-lysine (PL) lines have been printed on the electrode array for electrical studies on cultured neural networks. Rat hippocampal neurons showed a high degree of attachment selectivity to the PL and produced neurites that faithfully grew onto the electrode recording sites.
authors.affiliation: School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: James
authors.forename: R
authors.initials: R
authors.lastname: Davis
authors.forename: M
authors.initials: M
authors.lastname: Meyer
authors.forename: A
authors.initials: A
authors.lastname: Turner
authors.forename: S
authors.initials: S
authors.lastname: Turner
authors.forename: G
authors.initials: G
authors.lastname: Withers
authors.forename: L
authors.initials: L
authors.lastname: Kam
authors.forename: G
authors.initials: G
authors.lastname: Banker
authors.forename: H
authors.initials: H
authors.lastname: Craighead
authors.forename: M
authors.initials: M
authors.lastname: Isaacson
authors.forename: J
authors.initials: J
authors.lastname: Turner
authors.forename: W
authors.initials: W
authors.lastname: Shain
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002455:Cell Division; D006624:Hippocampus / Q000166:cytology; D008839:Microelectrodes; D009431:Neural Conduction; D009474:Neurons / Q000166:cytology*; D011107:Polylysine; D051381:Rats; D013499:Surface Properties
chemical_list: D011107:Polylysine