Profile of cytokine production during the inhibition of acute xenograft rejection.
Th2 cells have been shown to exert cell-mediated immune responses in vitro; therefore, the present study was conducted to investigate the cellular mechanisms of the long-term survival of cardiac xenografts by measuring cytokine production from recipient lymphocytes. Xenogeneic heart grafts from Lewis rats were transplanted into C57BL/6 mice, and the recipients were intravenously injected with H57-597 at the time of transplantation. The production of Th1 cytokines, namely, interleukin (IL)-2 and interferon (IFN)-gamma, and Th2 cytokines, namely, IL-4 and IL-10, from mixed lymphocyte culture cells was assayed by enzyme-linked immunosorbent assay. Splenocytes from the xenograft recipients treated with H57-597 produced significantly lower levels of IL-2, IFN-gamma, IL-4, and IL-10 than those from the untreated recipients, 100 days after transplantation. The findings of this study indicate that the long-term survival of cardiac xenografts induced by H57-597 is mediated by the inhibition of Th0, or both the Th1 and Th2 response.
Kishimoto
K
K
First Department of Surgery, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Furukawa
K
K
Ashizuka
S
S
Sawada
T
T
Ayabe
H
H
D000208:Acute Disease; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D006085:Graft Survival; D016027:Heart Transplantation / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007378:Interleukins / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051381:Rats; D011917:Rats, Inbred Lew; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D007378:Interleukins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D007371:Interferon-gamma