title: Proinflammatory activation of neutrophils and monocytes by Helicobacter pylori is not associated with cagA, vacA or picB genotypes.
abstract: Chronic Helicobacter pylori infection is associated with mucosal inflammation. The aim of the present study was to assess human neutrophil and monocyte activation induced by H. pylori strains with different virulence genotypes. Bacterial sonicates from 12 strains were used to induce phagocyte up-regulation of adherence molecule CD11b, assessed by fluorescence flow cytometry, and oxidative burst responses, assessed by chemiluminescence. A dose-dependent induction of the expression of CD11b was observed with sonicate from all H. pylori strains on both neutrophils and monocytes. Strains negative for cagA and picB genes had the same inducing activity of upregulation of CD11b as strains positive for these genes. A vacA-S2 type strain had the same activity as vacA-S1 type strains. The induction of toxic oxygen radicals by H. pylori-activated neutrophils gave higher median values for the cagA-positive strains than for the cagA-negative strains. For the monocyte chemiluminescence response, cagA-negative strains gave higher median values compared to cagA-positive strains. We conclude that upregulation of the neutrophil and monocyte adherence molecule CD11b induced by H. pylori sonicates is not associated with the presence of cagA, picB or mosaic pattern of vacA, and that cagA, picB-negative strains and vacA-S2 strains retain their inflammatory capacity.
authors.affiliation: Department of Clinical Immunology, Aalborg Hospital, Denmark.
authors.forename: P S
authors.initials: PS
authors.lastname: Hansen
authors.forename: M F
authors.initials: MF
authors.lastname: Go
authors.forename: K
authors.initials: K
authors.lastname: Varming
authors.forename: L P
authors.initials: LP
authors.lastname: Andersen
authors.forename: D Y
authors.initials: DY
authors.lastname: Graham
authors.forename: H
authors.initials: H
authors.lastname: Nielsen
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005756:Gastritis / Q000209:etiology; D005798:Genes, Bacterial; D005838:Genotype; D016481:Helicobacter Infections / Q000209:etiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000209:etiology*; D016177:Macrophage-1 Antigen / Q000378:metabolism; D009000:Monocytes / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D016897:Respiratory Burst; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D016177:Macrophage-1 Antigen; C103937:PicB protein, Helicobacter pylori; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori