Proteome map of the human hippocampus.
The proteins expressed by a genome have been termed the proteome. By comparing the proteome of a disease-affected tissue with the proteome of an unaffected tissue it is possible to identify proteins that play a role in a disease process. The hippocampus is involved in the processing of short-term memory and is affected in Alzheimer's disease. Any comparative proteome analysis that can identify proteins important in a disease affecting the hippocampus requires the characterization of the normal hippocampal proteome. Therefore, we homogenised normal hippocampal tissue and separated the proteins by two-dimensional polyacrylamide gel electrophoresis (2DE). Seventy-two unique protein spots were collected from Coomassie blue-stained 2DE gels and subjected to in-gel digestion with trypsin, reversed-phase high-pressure liquid chromatography peptide separation, and N-terminal protein sequencing. Sufficient protein sequence was obtained to successfully characterize 66 of the 72 protein spots chosen (92%). Three of the 66 proteins were not present in any database (4.5%). The characterized proteins comprised two dominant functional groups, i.e., enzymes involved in intermediary cellular metabolism (40%), and proteins associated with the cytoskeleton (15%). The identity, molecular mass, isoelectric point, and relative concentration of the characterized proteins are described and constitute a partial proteome map of the normal human hippocampus.
Edgar
P F
PF
School of Biological Sciences, University of Auckland, New Zealand. p.edgar@auckland.ac.nz
Douglas
J E
JE
Knight
C
C
Cooper
G J
GJ
Faull
R L
RL
Kydd
R
R
D000328:Adult; D001931:Brain Mapping; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015870:Gene Expression; D006624:Hippocampus / Q000737:chemistry*; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D020543:Proteome / Q000032:analysis*; D012016:Reference Values
D009419:Nerve Tissue Proteins; D020543:Proteome