The late developmental pattern of Mu transposon excision is conferred by a cauliflower mosaic virus 35S -driven MURA cDNA in transgenic maize.
The MuDR element responsible for Mutator activities in maize encodes two genes, mudrA and mudrB. Each encodes multiple transcripts hypothesized to regulate, directly or indirectly, the unique late timing and switch in transposition mechanism during maize development. mudrA, which encodes the MURA transposase, is unstable in bacterial plasmids, a technical problem solved by using phage M13 as a vector to prepare DNA for biolistic transformation. In transgenic maize, a single 2.7-kb mudrA cDNA predicted to encode an 823-amino acid protein is sufficient to catalyze late somatic excisions, despite removal of the native promoter, alternative transcription start sites, known introns, polymorphic 5' and 3' untranslated sequences, and the mudrB gene. These results suggest that post-translational regulation confers Mu excision timing. The transgene is active in lines containing silencing MuDR elements. This suggests that endogenous MuDR transposons do not measurably immunize the host against expression of a homologous transgene.
Raizada
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MN
Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA.
Walbot
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D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D017796:Caulimovirus / Q000235:genetics*; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development / Q000821:virology*
D017931:DNA Primers; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant