Interferences with semen detection by an immunoassay for a seminal vesicle-specific antigen.
A commercial enzyme-linked immunosorbent assay (ELISA), the SEMA assay, for a seminal vesicle-specific antigen (SVSA) provides highly sensitive detection of semen. Here we show marked interference of proteins such as albumin, serum proteins, or mucin with the assay. This would substantially decrease the sensitivity for detecting semen mixed with other biological fluids such as blood or vaginal secretions.
Chen
J T
JT
Clinical Pathology Department, National Institutes of Health, Bethesda, MD 20892-1508, USA.
Hortin
G L
GL
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008297:Male; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012661:Semen; D029589:Seminal Plasma Proteins; D012680:Sensitivity and Specificity; D014626:Vaginal Smears
D029584:Prostatic Secretory Proteins; D011506:Proteins; D011933:Reagent Kits, Diagnostic; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein