Establishment and characterization of a new human megakaryoblastic cell line (SET-2) that spontaneously matures to megakaryocytes and produces platelet-like particles.
A new factor-independent megakaryoblastic cell line, designated SET-2, was established from the peripheral blood of a patient with leukemic transformation of essential thrombocythemia (ET). SET-2 expressed CD 4, 7, 13, 33, 34, 36, 38, 41, 61, 71, 117, 126, 130 and c-mpl. In addition, it spontaneously produced numerous platelet-like particles in liquid culture. These particles were shown to be the same size as normal platelets, and to express CD 36, 38, 41, 61 and 71. Proliferation of SET-2 was not influenced by thrombopoietin (TPO) and other hemopoietic cytokines. SET-2 was found to express the platelet-specific proteins such as platelet factor 4 and beta-thromboglobulin. The levels of expression were not altered by TPO. SET-2 also secreted interleukin-6 into the supernatants, as well as normal megakaryocytes. These results suggest that SET-2 spontaneously matures to megakaryocytes and produces platelet-like particles. These findings indicate that SET-2 may be useful for investigating the proliferation and differentiation mechanisms of leukemia cells and the role of c-mpl on megakaryoblasts, megakaryocytes, and platelets in ET. Leukemia (2000) 14, 142-152.
Uozumi
K
K
Second Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.
Otsuka
M
M
Ohno
N
N
Moriyama
T
T
Suzuki
S
S
Shimotakahara
S
S
Matsumura
I
I
Hanada
S
S
Arima
T
T
D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D001792:Blood Platelets / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D015850:Interleukin-6 / Q000378:metabolism; D008533:Megakaryocytes / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013921:Thrombocytopenia / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D013926:Thrombopoietin / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D016207:Cytokines; D015850:Interleukin-6; D012333:RNA, Messenger; D013926:Thrombopoietin