title: The gene for human TATA-binding-protein-associated factor (TAFII) 170: structure, promoter and chromosomal localization.
abstract: The TATA-binding protein (TBP) plays a central role in eukaryotic transcription and forms protein complexes with TBP-associated factors (TAFs). The genes encoding TAF(II) proteins frequently map to chromosomal regions altered in human neoplasias. TAF(II)170 of B-TFIID is a member of the SF2 superfamily of putative helicases. Members of this superfamily have also been implicated in several human genetic disorders. In this study we have isolated human genomic clones encoding TAF(II)170 and we show that the gene contains 37 introns. Ribonuclease-protection experiments revealed that TAF(II)170 has multiple transcription start sites, consistent with the observation that the promoter lacks a canonical TATA box and initiator element. Deletion analysis of the promoter region showed that a fragment of 264 bp is sufficient to direct transcription. In addition, we determined the chromosomal localization by two independent methods which mapped the gene to human chromosome 10q22-q23 between the markers D10S185 and WI-1183. The region surrounding these markers has been implicated in several human disorders.
authors.affiliation: Laboratory for Physiological Chemistry and Center for Biomedical Genetics, Utrecht University, P.O. Box 80042, 3508 TA Utrecht, The Netherlands.
authors.forename: J A
authors.initials: JA
authors.lastname: Van Der Knaap
authors.forename: V
authors.initials: V
authors.lastname: Van Den Boom
authors.forename: J
authors.initials: J
authors.lastname: Kuipers
authors.forename: M J
authors.initials: MJ
authors.lastname: Van Eijk
authors.forename: P C
authors.initials: PC
authors.lastname: Van Der Vliet
authors.forename: H T
authors.initials: HT
authors.lastname: Timmers
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016385:TATA Box; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C109035:BTAF1 protein, human; D011994:Recombinant Proteins; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII