[The capacity of the mycelia of Streptomyces chrysomallus variants to bind exogenous actinomycin D and the synthesis by these variants of macrotetralides].
Streptomyces chrysomallus is known as an organism producing macrotetrolides (MTL) and actinomycin C. The dynamics of the MTL biosynthesis by some variants of S. chrysomallus in the process of their growth in liquid media was studied. In parallel the ability of the culture mycelium (washed or suspended in physiological solution) to bind exogenous actinomycin D (AMD) was estimated. An inverse correlation between the dynamics of MTL biosynthesis and the rate of the AMD binding by the washed mycelium during the whole period of the culture development was observed: a decrease in the culture ability to bind AMD corresponded to active biosynthesis of MTL and an increase in the culture ability to bind AMD corresponded to lower biosynthesis of MTL. It was suggested that the active biosynthesis of MTL correlated not only with a decrease in the ability of the suspended mycelium to bind AMD but also with a decrease in binding of actinomycin synthesized and excreted to the medium by the culture. A decrease in the reflux of the synthesized antibiotic to the cells was likely one of the components of the system of the S. chrysomallus insensitivity to its own antibiotic.
Orlova
T I
TI
M. V. Lomonosov Moscow State University.
Bulgakova
V G
VG
Grushina
V A
VA
Polin
A N
AN
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000493:pharmacokinetics*; D003470:Culture Media; D003609:Dactinomycin / Q000493:pharmacokinetics*; D014644:Genetic Variation / Q000502:physiology*; D018942:Macrolides; D013053:Spectrophotometry; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D003470:Culture Media; D018942:Macrolides; D003609:Dactinomycin