title: High-performance liquid chromatographic determination of pyrazinamide in human plasma, bronchoalveolar lavage fluid, and alveolar cells.
abstract: A technique is presented for the measurement of pyrazinamide in human plasma, bronchoalveolar lavage, and alveolar cells by reversed-phase column chromatography. The assay utilizes acetazolamide as an internal standard, ultraviolet detection at 268 nm, and an acetonitrile-based mobile phase. Preparation of plasma samples requires a simple deproteinization step, resulting in the development of sharp peaks with retention times of 8.4 and 17 minutes for pyrazinamide and acetazolamide, respectively. Bronchoalveolar lavage and alveolar cell suspensions require an acid extraction with ethyl acetate, evaporation to dryness, and reconstitution. This method provides specific, rapid, and reliable determinations of drug concentrations and therefore is suitable for pharmacological studies, particularly those that are designed to quantitate the intrapulmonary concentrations of pyrazinamide.
authors.affiliation: Department of Epidemiology & Biostatistics, University of California San Francisco 94117, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Conte
authors.forename: E
authors.initials: E
authors.lastname: Lin
authors.forename: E
authors.initials: E
authors.lastname: Zurlinden
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis* / Q000097:blood; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011650:Pulmonary Alveoli / Q000737:chemistry*; D011718:Pyrazinamide / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results
chemical_list: D000995:Antitubercular Agents; D011718:Pyrazinamide