title: [Acute respiratory Chlamydia pneumoniae infections in adults. Value of direct gene amplification diagnosis].
abstract: Chlamydia pneumoniae has been established recently as an important human respiratory pathogen. The aim of this study was to define the incidence of Chlamydia pneumoniae in acute respiratory infections by evaluating its presence in posterior nasopharyngeal aspirates or broncho-alveolar lavage specimens by polymerase chain reaction-hybridization (PCR-EIA) as well as the titres of specific antibodies in serum by a rELISA test and a micro-immunofluorescence (MIF) test. 68 adults patients were investigated. Eight patients (11.8%) were positive by either rELISA or PCR-EIA or both, with an infection rate of 5 patients with community-acquired pneumonia, 2 asthmatic patients and 1 patients with an exacerbation of chronic obstructive pulmonary disease. Serological evidence of acute infection was found in four of these patients with rELISA test and in three others with MIF test. PCR-EIA detected Chlamydia pneumoniae DNA in four patients, but there were concordant results with rELISA and PCR-EIA in only one patient. In conclusion, Chlamydia pneumoniae appears to be a common etiologic agent of acute respiratory infections in adults. The discrepancy between serological test and PCR-EIA results reflects the difficulties in routine laboratory diagnosis of Chlamydia pneumoniae. The ambiguous results of serological tests from a single serum sample assess the utility of PCR for prompt diagnosis. When PCR is negative or no feasible, a second serology to 15/21 days of interval is necessary. Further studies with optimised techniques must be developed.
authors.affiliation: Service de Pneumologie, Hôpital Côte de Nacre, CHRU de Caen. bellini@club-internet.fr
authors.forename: F
authors.initials: F
authors.lastname: Vincent
authors.forename: J
authors.initials: J
authors.lastname: Petitjean
authors.forename: J E
authors.initials: JE
authors.lastname: Filmont
authors.forename: G
authors.initials: G
authors.lastname: Le Moël
authors.forename: V
authors.initials: V
authors.lastname: Fontaine
authors.forename: A
authors.initials: A
authors.lastname: Vabret
authors.forename: F
authors.initials: F
authors.lastname: Freymuth
authors.forename: J
authors.initials: J
authors.lastname: Brun
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000032:analysis; D001249:Asthma / Q000150:complications; D002690:Chlamydia Infections / Q000150:complications / Q000175:diagnosis*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology; D017714:Community-Acquired Infections / Q000175:diagnosis; D004269:DNA, Bacterial / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008171:Lung Diseases / Q000150:complications; D008173:Lung Diseases, Obstructive / Q000150:complications; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000150:complications / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D004269:DNA, Bacterial