title: A novel form of prostate-specific antigen transcript produced by alternative splicing.
abstract: Molecular characterization of prostate-specific antigen (PSA) has not been well elucidated, despite a great deal of clinical study. We examined the heterogeneity of PSA using reverse transcription-PCR and direct sequencing. A novel, alternatively spliced variant of the PSA transcript was found in prostate cancer (PC), as well as in benign prostatic tissue. This alternative splicing leads to the deletion of 44 amino acid residues (amino acids 45-88) from mature PSA, resulting in the loss of asparagine 45, which is a binding site for a carbohydrate chain. By these nested reverse transcription-PCR systems, this novel, alternatively spliced PSA gene was recognized in 13 of 18 (72.2%) cases with noncancerous prostate tissue, 4 of 5 (80.0%) PC cases, and 3 of 12 (25.0%) blood samples from PC patients (noncancerous prostate tissue group versus blood sample group, P = 0.011). At present, the biological significance of this alternative splicing remains to be established.
authors.affiliation: Department of Urology, Shiga University of Medical Science, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Isono
authors.forename: T
authors.initials: T
authors.lastname: Yoshiki
authors.forename: T
authors.initials: T
authors.lastname: Yuasa
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D015153:Blotting, Western; D003937:Diagnosis, Differential; D015870:Gene Expression; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics; D017430:Prostate-Specific Antigen / Q000737:chemistry* / Q000235:genetics; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000175:diagnosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D009363:Neoplasm Proteins; D017430:Prostate-Specific Antigen