New (15)N NMR exchange experiments for the unambiguous assignment of (1)H(N)/(15)N resonances of proteins in complexes in slow chemical exchange with free form.
The potentialities of a 2D proton-detected heteronuclear exchange experiment to assign the nitrogen and amide proton resonances in a uniformly (15)N-enriched macromolecule involved in a complex, starting from the free form assignments, are demonstrated on a protein-DNA complex. This 2D experiment is further extended to a 3D experiment in the case of severe superpositions.
Vialle-Printems
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C
Laboratoire de RMN, ICSN-CNRS, 1 Avenue de la Terrasse, Gif-sur-Yvette, F-91198, France.
van Heijenoort
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C
Guittet
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E
D004247:DNA / Q000737:chemistry; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D046911:Macromolecular Substances; D011506:Proteins; D004247:DNA