Inhibitors of RT-PCR in serum.
Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
Konet
D S
DS
Plum Island Animal Disease Center, Greenport, NY 11944, USA.
Mezencio
J M
JM
Babcock
G
G
Brown
F
F
D000818:Animals; D005537:Aphthovirus / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D005536:Foot-and-Mouth Disease / Q000821:virology; D005784:Gene Amplification; D006168:Guinea Pigs; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013552:Swine; D014773:Virology / Q000379:methods*
D017931:DNA Primers; D012367:RNA, Viral