Up-regulation of crystallin mRNAs in form-deprived chick eyes.
Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-regulated in the retina-RPE-choroid of form-deprived chick eyes. A suppression subtractive hybridization method was used to compare mRNA expression in the retina-RPE-choroid of form-deprived and control eyes. One up-regulated cDNA was isolated and identified as part of chick delta1-crystallin cDNA. Northern blot and RT-PCR analyses demonstrated that delta1-crystallin mRNA was up-regulated in the retina-RPE at day 7 after form-deprivation treatment. Semi-quantitative RT-PCE analysis of the expression of several transcription factors indicated that Sox1 and Sox3 were upregulated in parallel with delta1-crystallin mRNA in form-deprived eyes. Northern blot analysis demonstrated that alphaA-, betaA3/A1-, betaB1-, and betaB2-crystallin mRNAs were also up-regulated in form-deprived eyes. Although the detailed mechanisms and functions of the crystallin family genes in the retina-RPE-choroid of form-deprived eyes remain unclear, results of our study suggests that form-deprivation affects the expression of these genes in chick eyes.
Ishibashi
K
K
Department of Ophthalmology, Kobe University School of Medicine, Kobe, Japan. kazuki@med.kobe-u.ac.jp
Fujii
S
S
Escaño
M F
MF
Sekiya
Y
Y
Yamamoto
M
M
D000818:Animals; D015152:Blotting, Northern; D002645:Chickens; D003459:Crystallins / Q000235:genetics / Q000378:metabolism*; D005556:Form Perception / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D012683:Sensory Deprivation / Q000502:physiology; D015854:Up-Regulation
D003459:Crystallins; D012333:RNA, Messenger