Expression of elastin-related proteins and matrix metalloproteinases in actinic elastosis of sun-damaged skin.
Actinic elastosis is characterized by an accumulation of elastotic material in the upper dermis and is considered to be a manifestation of ultraviolet-induced skin aging. To compare the structural components of the elastotic material in actinic elastosis with those in normal skin, skin specimens were stained with antibodies raised against various elastin-related proteins. Elastotic materials exhibited a strong reaction to the antibodies for elastin, microfibril-associated glycoprotein-1 (MAGP-1), MAGP-4, matrix metalloproteinase 1 (MMP-1), MMP-2 and MMP-3, but a diminished reaction to anti-MMP-9 antibody. Fibroblast cell lines from the upper dermis of affected and unaffected skin were established, and the mRNA levels of MMPs were determined. MMP-1 and -2 mRNA levels were found to be elevated approximately twofold in the fibroblasts from actinic elastosis. Since MMP-1 and -2 are considered to be major enzymes involved in the degradation of matrix components, the accumulation of elastotic materials in actinic elastosis may be related to the degradation process.
Ohnishi
Y
Y
Department of Dermatology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359, Japan.
Tajima
S
S
Akiyama
M
M
Ishibashi
A
A
Kobayashi
R
R
Horii
I
I
D000368:Aged; D000369:Aged, 80 and over; D015152:Blotting, Northern; D004547:Elastic Tissue / Q000737:chemistry / Q000473:pathology*; D004549:Elastin / Q000032:analysis* / Q000096:biosynthesis; D005260:Female; D000071837:Fibrillins; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D006801:Humans; D007150:Immunohistochemistry; D020781:Matrix Metalloproteinase 1 / Q000032:analysis / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000235:genetics; D019278:Matrix Metalloproteinase 3 / Q000032:analysis; D020782:Matrix Metalloproteinases / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D008840:Microfilament Proteins / Q000032:analysis; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012867:Skin / Q000737:chemistry / Q000473:pathology / Q000528:radiation effects*; D015595:Skin Aging / Q000473:pathology / Q000528:radiation effects; D012871:Skin Diseases / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D013472:Sunlight / Q000009:adverse effects
D000071837:Fibrillins; D008840:Microfilament Proteins; D012333:RNA, Messenger; D004549:Elastin; D020782:Matrix Metalloproteinases; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1