Broadening of coreceptor usage by human immunodeficiency virus type 2 does not correlate with increased pathogenicity in an in vivo model.
The pathogenic properties of four primary human immunodeficiency virus type 2 (HIV-2) isolates and two primary HIV-2 biological clones were studied in an in vivo human-to-mouse chimeric model. The cell-associated viral load and the ability to reduce the severity of the induced graft-versus-host disease symptoms, the CD4/CD8 ratio and the level of repopulation of the mouse tissues by the graft, were determined. All HIV-2 strains, irrespective of their in vitro biological phenotype, replicated to high titres and significantly reduced graft-versus-host disease symptoms as well as the CD4/CD8 ratios. Reduction of graft repopulation caused by infection with the respective HIV-2 strains showed that the in vitro replication rate, syncytium-inducing capacity and ability to infect human macrophages did influence the in vivo pathogenic potential whereas broadening of coreceptor usage did not.
van Der Ende
M E
ME
Department of Virology, Erasmus Medical Centre Rotterdam, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.
Guillon
C
C
Boers
P H
PH
Gruters
R A
RA
Racz
P
P
Tenner-Racz
K
K
Osterhaus
A D
AD
Schutten
M
M
D000208:Acute Disease; D000818:Animals; D016516:CD4-CD8 Ratio; D002678:Chimera; D004195:Disease Models, Animal; D006086:Graft vs Host Disease / Q000276:immunology; D015658:HIV Infections / Q000209:etiology* / Q000276:immunology / Q000821:virology; D015498:HIV-2 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D051379:Mice; D015728:Receptors, HIV / Q000502:physiology*; D014183:Transplantation, Heterologous; D014779:Virus Replication
D015728:Receptors, HIV