Effect of exhaustive exercise on membrane estradiol concentration, intracellular calcium, and oxidative damage in mouse thymic lymphocytes.
Early Ca2+ signaling events in cells of the immune system after exhaustive exercise challenge (8% slope, 32 m/min(-1) speed) of female C57BL/6 mice, and their effects on oxidative reactions in thymus were studied. Intracellular Ca2+ and the oscillation of free extracellular Ca2+ were imaged with cell permeant cell and cell impermeant Fluo 3 calcium indicator in thymocytes. The role of estradiol was assessed by RIA for levels of membrane bound estradiol. Oxidative product release and membrane lipid peroxide were also evaluated. Intracellular Ca2+ levels were significantly higher in thymocytes of exercised compared with control mice (p < .001). There was a continuous flux of Ca2+ after exercise when cells were monitored in Ca2+ rich medium, with a significant influx between 160 and 200 sec (p < .001). Membrane bound estradiol was elevated in thymocytes of exercised compared to control mice (p < .05). Immediately after exercise there was a greater release of oxidative products by thymocytes in exhaustively exercised compared with control animals. There was also significant generation of lipid peroxide in thymus of exercised mice (p < .001). The findings suggest that exhaustive exercise may stimulate estradiol uptake by receptors on thymocytes, with a possible opening up of estradiol-receptor operated channels for Ca2+ entry into cells. This may have damaging effects on thymic lymphocytes by the triggering of oxidative reactions as determined by higher oxidative product release and greater generation of lipid peroxide.
Azenabor
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Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, Ontario, Canada.
Hoffman-Goetz
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D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007424:Intracellular Fluid / Q000378:metabolism; D015640:Ion Channel Gating; D017136:Ion Transport; D015227:Lipid Peroxidation; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D011960:Receptors, Estrogen / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology*
D015220:Calcium Channels; D011960:Receptors, Estrogen; D004958:Estradiol; D002118:Calcium