title: Nitric oxide influences injury-induced microglial migration and accumulation in the leech CNS.
abstract: Damage to the leech or mammalian CNS increases nitric oxide (NO) production and causes accumulation of phagocytic microglial cells at the injury site. The aim of this study was to determine whether NO plays a role in microglial migration and accumulation at lesions in which NO is generated by a rapidly appearing endothelial nitric oxide synthase (eNOS) in leeches. Immunohistochemistry and cytochemistry demonstrated active eNOS before and throughout the period of microglial accumulation at the lesion. Decreasing NO synthesis by application of the NOS inhibitor N(w)-nitro-L-arginine methyl ester (1 mM) significantly reduced microglial accumulation, whereas its inactive enantiomer N(w)-nitro-D-arginine methyl ester (1 mM) resulted in microglial accumulation similar to that in crushed controls. Increasing NO with the donor spermine NONOate (SPNO) (1 mM) also inhibited accumulation, but not in the presence of the NO scavenger 2-(4-carboxyphenyl)-4,4,5, 5-teramethylimidazoline-oxyl-3-oxide (50 microM). The effect of SPNO was reversed by washout. SPNO application reduced average microglial migratory speeds and even reversibly arrested cell movement, as measured in living nerve cords. These results suggest that NO produced at a lesion may be a stop signal for microglia to accumulate there and that it can act on microglia early in their migration. Thus, NO may assume a larger role in nerve repair and recovery from injury by modulating accumulation of microglia, which appear to be important for axonal regeneration.
authors.affiliation: Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33136, USA.
authors.forename: A
authors.initials: A
authors.lastname: Chen
authors.forename: S M
authors.initials: SM
authors.lastname: Kumar
authors.forename: C L
authors.initials: CL
authors.lastname: Sahley
authors.forename: K J
authors.initials: KJ
authors.lastname: Muller
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002490:Central Nervous System / Q000293:injuries*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007865:Leeches; D017628:Microglia / Q000187:drug effects / Q000473:pathology* / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009409:Nerve Crush; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D009589:Nitrogen Oxides; D013096:Spermine / Q000031:analogs & derivatives / Q000494:pharmacology; D014947:Wounds and Injuries / Q000473:pathology* / Q000503:physiopathology*
chemical_list: D004791:Enzyme Inhibitors; D020030:Nitric Oxide Donors; D009589:Nitrogen Oxides; C091861:spermine nitric oxide complex; D013096:Spermine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester