Influence of anti-allergic agents on in vivo expression of co-stimulatory molecules in normal mice.
The co-stimulatory signal provided by the interaction between CD28 and its ligands, CD80 and CD86, is critical for T cell activation. Engagement of CD40 and CD40L is also essential for IgE synthesis and secretion. In the present study, we examined the influence of anti-allergic drugs on co-stimulatory molecule (CD40, CD80 and CD86) expression in normal mouse splenocytes by flow cytometry. Treatment of BALB/c mice with azelastine (AZ) at a dose of 50.0 micrograms/kg/day, which is the most effective therapeutic dose, for two weeks scarcely affected the expression of co-stimulatory molecules CD40, CD80 and CD86 on splenocytes, whereas a three-week treatment strongly suppressed the expression of these molecules. We also examined the influence of terfenadine (TR) on co-stimulatory molecule expression. The expression of molecules on splenocytes was inhibited when donor mice were treated orally with 2.0 mg/kg/day of TR for three weeks. However, it was not inhibited during a two-week period treatment. These results suggest that the attenuating effects of anti-allergic agents on the diseases may be explained, in part, by their inhibitory action of co-stimulatory molecule expression.
Tryka
E
E
Department of Otolaryngology, School of Medicine, Showa University, Tokyo, Japan.
Asano
K
K
Ito
J
J
Hisamitsu
T
T
Suzaki
H
H
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D015703:Antigens, CD / Q000096:biosynthesis*; D018122:B7-1 Antigen / Q000096:biosynthesis; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000096:biosynthesis; D005434:Flow Cytometry; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010793:Phthalazines / Q000494:pharmacology*; D016593:Terfenadine / Q000494:pharmacology*
D018926:Anti-Allergic Agents; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497965:Cd86 protein, mouse; D008562:Membrane Glycoproteins; D010793:Phthalazines; D016593:Terfenadine; C020976:azelastine