Introduction of 6-formylcytidine into a Myb binding sequence.
Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3'-TTGAC-5'. The modified duplex showed Tm of 67 degrees C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.
Kittaka
A
A
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Kuze
T
T
Amano
M
M
Tanaka
H
H
Miyasaka
T
T
Hirose
K
K
Yoshida
T
T
Sarai
A
A
Yasukawa
T
T
Ishii
S
S
D001665:Binding Sites; D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000737:chemistry; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013696:Temperature
C403912:6-formylcytidine; D009838:Oligodeoxyribonucleotides; D020598:Proto-Oncogene Proteins c-myb; D003562:Cytidine; D008239:Lysine