title: Full cytogenetic characterization of a new neuroblastoma cell line with a complex 17q translocation.
abstract: Recent studies have shown that structural abnormalities of chromosome 17 resulting in gain of material are the most frequent genetic changes in neuroblastoma. We have established a new neuroblastoma cell line from a patient whose disease had evolved from stage 4s to 4, without evidence of deletion of the short arm of chromosome 1 and MYCN amplification, which are considered the most typical genetic indicators of aggressive disease. The cytogenetic study allowed a full characterization of the chromosome changes, and revealed a complex translocation of chromosome 17 leading to a derivative marker which may be described as follows: der(11)t(11;17)(p15;q12)t(11;17) (q22;q12). This resulted in a gain of part of the long arms of chromosome 17, which was recently associated with poor prognosis.
authors.affiliation: Divisione di Ematologia ed Oncologia Pediatrica, Istituto Giannina Gaslini, Genova, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Panarello
authors.forename: C
authors.initials: C
authors.lastname: Morerio
authors.forename: I
authors.initials: I
authors.lastname: Russo
authors.forename: F
authors.initials: F
authors.lastname: Pasquali
authors.forename: A
authors.initials: A
authors.lastname: Rapella
authors.forename: M V
authors.initials: MV
authors.lastname: Corrias
authors.forename: A
authors.initials: A
authors.lastname: Morando
authors.forename: C
authors.initials: C
authors.lastname: Rosanda
mesh_terms: D000818:Animals; D002886:Chromosomes, Human, Pair 17; D005455:Fluorescent Antibody Technique; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000473:pathology*