Characterization of TEM-56, a novel beta-lactamase produced by a Klebsiella pneumoniae clinical isolate.
TEM-56 produced by a Klebsiella pneumoniae clinical isolate is a novel beta-lactamase of isoelectric point 6.4 that confers a moderate resistance level to expanded-spectrum cephalosporins. The amino acid sequence deduced from the corresponding bla gene showed two amino acid replacements with respect to the TEM-2 sequence: Glu-104 to Lys and His-153 to Arg. This enzyme showed catalytic properties close to those of TEM-18. Thus, TEM-56 appears as a new TEM mutant, an intermediary between TEM-18 and the extended-spectrum beta-lactamase TEM-21.
Neuwirth
C
C
Laboratoire de Bact¿eriologie, H¿opital Universitaire du Bocage, B.P. 1542, 21034 Dijon Cedex, France.
Labia
R
R
Siebor
E
E
Pechinot
A
A
Madec
S
S
Chaibi
E B
EB
Kazmierczak
A
A
D019943:Amino Acid Substitution; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000378:metabolism*
C405500:TEM-56 beta-lactamase; D001618:beta-Lactamases