title: PCB congener profile in the serum of humans consuming Great Lakes fish.
abstract: The State of Michigan has a long history of research into human exposure to environmental contaminants through consumption of recreationally caught fish. A large cohort of Lake Michigan residents who eat fish (fish-eaters) and those who do not eat fish (nonfish-eaters) established in 1980 served as the basis for the congener-specific polychlorinated biphenyl (PCB) exposure evaluation reported here. In this paper we present the serum PCB congener profile for a subset of this cohort who were over 50 years of age. Serum samples were collected in 1993-1995 and were evaluated by a dual column capillary column gas chromatography procedure capable of detecting over 90 PCB congeners. This evaluation demonstrated significant PCB exposure in the fish-eaters (mean serum PCB of 14.26 ppb; n = 101). This elevated exposure allowed the establishment of a detailed profile of the PCB congeners found in humans exposed by this route. Twenty-two congeners of varying concentrations were the most prevalent and constituted over 95% of the total PCB present in most subjects. Four congeners, 138/163 (2,2',3,4,4',5-PCB/2,3,3',4', 5,6-PCB), 180 (2,2',3,4,4',5,5'-PCB), and 153 (2,2',4,4',5,5'-PCB), accounted for 55-64% of the total PCB load. Other congeners, some of toxicologic significance, were also detected by this analytical protocol. Nonfish-eaters had lower total serum PCB levels (mean = 4. 56; n = 78), but the same general pattern of PCB congeners was present. It was demonstrated that careful selection of a subset of prevalent PCB congeners could provide a cost-effective assessment of exposure without losing critical scientific information.
authors.affiliation: Institute of Environmental Toxicology, Michigan State University, East Lansing, Michigan, USA.
authors.forename: H E
authors.initials: HE
authors.lastname: Humphrey
authors.forename: J C
authors.initials: JC
authors.lastname: Gardiner
authors.forename: J R
authors.initials: JR
authors.lastname: Pandya
authors.forename: A M
authors.initials: AM
authors.lastname: Sweeney
authors.forename: D M
authors.initials: DM
authors.lastname: Gasior
authors.forename: R J
authors.initials: RJ
authors.lastname: McCaffrey
authors.forename: S L
authors.initials: SL
authors.lastname: Schantz
mesh_terms: D000368:Aged; D000818:Animals; D016022:Case-Control Studies; D002849:Chromatography, Gas; D015331:Cohort Studies; D004785:Environmental Pollutants / Q000097:blood*; D005260:Female; D005399:Fishes; D005506:Food Contamination; D006801:Humans; D008297:Male; D008824:Michigan / Q000453:epidemiology; D008875:Middle Aged; D011078:Polychlorinated Biphenyls / Q000097:blood*; D017747:Seafood
chemical_list: D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls