Restriction endonucleases: one of these things is not like the others.
The crystal structure of the restriction endonuclease BglII in complex with its DNA target site has been determined. The DNA binding mode and chemistry of catalysis are observed to differ from BamHI which cleaves a similar target site. These observations indicate that more divergence has occurred within this family of proteins than originally thought.
Galburt
E A
EA
Stoddard
B L
BL
D020816:Amino Acid Motifs; D001426:Bacterial Proteins; D001665:Binding Sites; D003461:Crystallography / Q000379:methods; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000737:chemistry / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D019143:Evolution, Molecular; D011487:Protein Conformation; D017510:Protein Folding
D001426:Bacterial Proteins; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; C048901:endodeoxyribonuclease CreI; C059776:endodeoxyribonuclease MfeI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases