Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A/PR/8 virus in MDCK cells.
Using several herbal extracts, we investigated whether certain Kampo medicines exert an inhibitory effect on the acidification of intracellular compartments such as endosomes and lysosomes (referred to as ELS), and thereby inhibit the growth of influenza A virus in Madin-Darby canine kidney cells. The vital fluorescence microscopic study showed that the extract of Ephedrae herba (EHext) among five herbal extracts inhibited acidification of endosomes and lysosomes in a concentration-dependent manner (100-400 microg/ml). Moreover the growth of influenza A/PR/8/34 (H1N1) (PR8) virus was inhibited when the cells were treated with EHext for 1 h immediately after infection, or treated as early as 5-10 min after infection. Conversely, virus growth resumed concomitantly with the reappearance of acidified ELS after removal of EHext. The fact that the inhibitory effect of EHext was completely or partially reversed by FeCl3, a tannin-reactive agent, strongly suggests that tannin is one of the active components in the extract.
Mantani
N
N
Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani.
Andoh
T
T
Kawamata
H
H
Terasawa
K
K
Ochiai
H
H
D000818:Animals; D002460:Cell Line; D002712:Chlorides; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D011992:Endosomes / Q000502:physiology; D005290:Ferric Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D047348:Hydrolyzable Tannins / Q000378:metabolism; D009980:Influenza A virus / Q000187:drug effects* / Q000254:growth & development; D007668:Kidney; D008247:Lysosomes / Q000502:physiology; D020835:Medicine, Kampo; D008856:Microscopy, Fluorescence
D002712:Chlorides; D004365:Drugs, Chinese Herbal; D005290:Ferric Compounds; D047348:Hydrolyzable Tannins; C024555:ferric chloride