title: In vitro and in vivo assessment of the Ventrak 1550/Capnogard 1265 for single breath carbon dioxide analysis in neonates.
abstract: The Ventrak 1550/Capnogard 1265 (V&C) enables deadspace (VD) measurements to be made in neonates. The aim of our studies was to validate the V&C device for VD measurement in vitro (lung model) and in vivo (adult rabbits). Methods of measurement of VD using the V&C (automatic computation, interactive carbon dioxide-volume plot analysis, Bohr equation) were tested by comparing known added deadspace volumes (VDadd) with calculated VDadd. After producing a change in alveolar (VDalv) and physiological (VDphys) deadspace by in vivo broncho-alveolar lavage, VDalv and VDphys computed automatically were compared with values calculated by the Bohr-Enghoff equations. VDadd was slightly underestimated (absolute error in mean: automatically -0.61 ml; interactively -0.55 ml; Bohr -0.54 ml). The higher the VDadd, the lower the absolute errors and coefficients of variation (cv). The highest cv occurred for automatic analysis (approximately 11%) compared with < 6% for interactive analysis or the Bohr equation. Average differences between results calculated automatically and by the Bohr-Enghoff equation were -0.79 ml for VDalv (95% confidence interval -2.02 to 0.44 ml) and -0.23 ml for VDphys (-0.6 to 0.14 ml). We conclude that the V&C can be used in newborn infants undergoing mechanical ventilation, if changes in VD are < 5 ml, interactive analysis or the Bohr equation should be used.
authors.affiliation: Clinic of Neonatology, Charité-Hospital, Humboldt University, Berlin, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Wenzel
authors.forename: R R
authors.initials: RR
authors.lastname: Wauer
authors.forename: M H
authors.initials: MH
authors.lastname: Wagner
authors.forename: G
authors.initials: G
authors.lastname: Schmalisch
mesh_terms: D019296:Capnography / Q000295:instrumentation*; D002245:Carbon Dioxide / Q000032:analysis*; D004195:Disease Models, Animal; D006801:Humans; D007224:Infant Care / Q000295:instrumentation*; D007231:Infant, Newborn; D008954:Models, Biological; D011650:Pulmonary Alveoli / Q000502:physiology; D012121:Respiration, Artificial; D012126:Respiratory Dead Space
chemical_list: D002245:Carbon Dioxide