Localization of monoamine oxidase A and B in human pancreas, thyroid, and adrenal glands.
We studied monoamine oxidase (MAO) A and B localization in human pancreas, thyroid gland, and adrenal gland by immunohistochemistry. The primary antibodies used were mouse monoclonal anti-human MAO-A (6G11/E1) and anti-human MAO-B (3F12/G10/2E3). Samples were obtained from six routine autopsy cases and fixed in 2% paraformaldehyde. Exocrine pancreas showed a widespread distribution of MAO-A, whereas MAO-B was present only in centroacinar cells and epithelial cells of pancreatic ducts. In endocrine pancreas, MAO-A was observed in around 50% of islet cells, whereas MAO-B was less abundant and was restricted to the periphery of islets. Thyroid gland showed strong MAO-A immunoreactivity in all cell types and was MAO-B-negative. In adrenal gland, the capsule displayed MAO-A but not MAO-B immunoreactivity, whereas the cortex showed widespread MAO-A staining but was MAO-B-negative in interstitial cells. Finally, in the medulla only a few scattered cells showed either MAO-A or MAO-B immunoreactivity. To our knowledge, these data represent the first study of the cellular distribution of MAO-A and MAO-B in the three human tissues included.
Rodríguez
M J
MJ
Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.
Saura
J
J
Finch
C C
CC
Mahy
N
N
Billett
E E
EE
D000311:Adrenal Glands / Q000201:enzymology*; D000368:Aged; D000369:Aged, 80 and over; D000918:Antibody Specificity; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000201:enzymology; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000276:immunology / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology*; D013961:Thyroid Gland / Q000201:enzymology*
D007527:Isoenzymes; D008995:Monoamine Oxidase