Two carbohydrate recognition domains of Hyphantria cunea lectin bind to bacterial lipopolysaccharides through O-specific chain.
We previously identified a novel lectin cDNA from the fall webworm [Shin et al. (1998) Insect Biochem. Mol. Biol. 28, 827-837], which encodes two carbohydrate recognition domains (CRD-N and CRD-C) and is up-regulated following bacterial challenge. The lipopolysaccharide (LPS) binding activities of the recombinant CRD-N and CRD-C (rCRD-N and rCRD-C) were investigated by enzyme-linked immunosorbent assay. The LPS binding of rCRD-N and rCRD-C was pH-dependent: at pH below 6.0, they show a higher binding ability to LPS. The binding of the rCRD-N was inhibited by both D-mannose and N-acetyl-D-glucosamine, whereas the binding of the rCRD-C was inhibited only by D-mannose. The binding of both rCRD-N and rCRD-C to Escherichia coli was mainly mediated through the O-specific chain.
Shin
S W
SW
Insect Resources Laboratory, Korea Research Institute of Bioscience and Biotechnology, 52 Eoun-Dong, Yusong, Taejon, South Korea.
Park
D S
DS
Kim
S C
SC
Park
H Y
HY
D000117:Acetylglucosamine / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D008823:Micelles; D008969:Molecular Sequence Data; D009036:Moths; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics
D016056:Immunodominant Epitopes; D037102:Lectins; D008823:Micelles; D019081:O Antigens; D011993:Recombinant Fusion Proteins; D008358:Mannose; D000117:Acetylglucosamine