title: Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria.
abstract: The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.
authors.affiliation: Laboratoire de Biologie Microbienne, Université de Lausanne, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Heeb
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Nishijyo
authors.forename: U
authors.initials: U
authors.lastname: Schnider
authors.forename: C
authors.initials: C
authors.lastname: Keel
authors.forename: J
authors.initials: J
authors.lastname: Wade
authors.forename: U
authors.initials: U
authors.lastname: Walsh
authors.forename: F
authors.initials: F
authors.lastname: O'Gara
authors.forename: D
authors.initials: D
authors.lastname: Haas
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010944:Plants / Q000382:microbiology*; D010957:Plasmids / Q000235:genetics; D011551:Pseudomonas fluorescens / Q000235:genetics*; D012093:Replicon / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004274:DNA, Recombinant