NMR study of volatile anesthetic binding to nicotinic acetylcholine receptors.
New lines of evidence suggest that volatile anesthetics interact specifically with proteins. Direct binding analysis, however, has been largely limited to soluble proteins. In this study, specific interaction was investigated between isoflurane, a clinically important volatile anesthetic, and membrane-bound nicotinic acetylcholine receptors (nAChRs) from Torpedo electroplax, using (19)F nuclear magnetic resonance spectroscopy and gas chromatography. The receptors were reconstituted into 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipid vesicles. After correcting for nonspecific partitioning into the lipid, the equilibrium dissociation constant, K(d), of isoflurane binding to nAChR at 15 degrees C was found to be 0.36 +/- 0.03 mM. This value is within the clinically relevant concentration range of the agent. Based on the receptor concentrations in the vesicle suspension assayed by the bicinchoninic acid method and the fraction of bound isoflurane, X(b), determined by gas chromatography, an estimate of an average of 9-10 specifically bound isoflurane molecules can be made for each receptor, or two for each subunit. Upon binding, the transverse relaxation time constant (T(2)) of (19)F resonance of isoflurane is decreased by nearly three orders of magnitude, indicating a dramatic reduction in the mobility of specifically bound isoflurane. Kinetic analysis reveals that the off rate of binding, k(-1), is 1.7 x 10(4) s(-1). The on rate, k(+1), can thus be calculated to be approximately 4.8 x 10(7) M(-1) s(-1), suggesting a nearly diffusion-limited association. This is in contrast to anesthetic binding to a soluble protein, bovine serum albumin (BSA), where k(+1) and k(-1) are at least an order of magnitude slower. It is concluded that the presence of lipids may be critical for the correct evaluation of binding kinetics between volatile anesthetics and neuronal receptors.
Xu
Y
Y
Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh, Pennsylvania 15261, USA. xu@smtp.anes.upmc.edu
Seto
T
T
Tang
P
P
Firestone
L
L
D018685:Anesthetics, Inhalation / Q000737:chemistry*; D000818:Animals; D002849:Chromatography, Gas; D007530:Isoflurane / Q000737:chemistry; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010713:Phosphatidylcholines / Q000737:chemistry; D011485:Protein Binding; D011510:Proteolipids / Q000737:chemistry; D011978:Receptors, Nicotinic / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014101:Torpedo
D018685:Anesthetics, Inhalation; D010713:Phosphatidylcholines; D011510:Proteolipids; D011978:Receptors, Nicotinic; D012710:Serum Albumin, Bovine; D007530:Isoflurane; C017251:1,2-oleoylphosphatidylcholine