Quantitative direct probe method for the detection of parvovirus B19.
Parvovirus B19 infection is associated with anemia and spontaneous abortions. While many qualitative assays are available, a few molecular-based quantitative methods have been described. This study reports the development and optimization of a quantitative direct-probe method for the detection of Parvovirus B19 DNA. Different concentrations of RNA probes were used to identify the optimal conditions for hybridizing to the target DNA. Detection of DNA was linear between concentrations of 2 ng/ml to 200 pg/ml. Because this method requires no enzymatic amplification, it is not susceptible to amplifier contamination or enzymatic inhibitors, and it can be applied to serum samples or paraffin-embedded tissue.
Boggino
H
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Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0743, USA.
Payne
D A
DA
D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000821:virology; D018076:DNA, Complementary; D004279:DNA, Viral / Q000032:analysis / Q000097:blood / Q000302:isolation & purification*; D019286:Endopeptidase K; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016612:Paraffin Embedding; D010322:Parvoviridae Infections / Q000175:diagnosis*; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D015347:RNA Probes; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D004279:DNA, Viral; D015347:RNA Probes; D011933:Reagent Kits, Diagnostic; D019286:Endopeptidase K