[Cardiolipin antibodies dependents on beta2-glycoprotein-1 in antiphospholipid syndrome].
OBJECTIVE
To study beta2-GP-I-dependent binding of phospholipid antibodies (PAb) to phospholipids and this process participation in pathogenesis of antiphospholipid syndrome (APS).


METHODS
IgG-fractions and sera from 20 patients with APS. Cofactor activity of beta2-GP-I isolated from serum of healthy donors was examined with modified immunoassay.


RESULTS
Contrary to donor IgG, binding of IgG fractions isolated from sera of APS patients with cardiolipin grows dose-dependently in the presence of beta2-GP-I. Cofactor activity of beta2-GP-I is confirmed in the study of sera of APS patients. Sera containing beta2-GP-I-dependent antibodies to cardiolipin (aCL), unlike aCL-negative sera, react with solid-phase immobilized beta2-GP-I.


CONCLUSIONS
It is confirmed that beta2-GP-I participates in interaction of PAb with cardiolipin. Pathogenetic implication of beta2-GP-I-dependent PAb for onset of APS is discussed.
Kuznetsova
T V
TV
Tishchenko
V A
VA
Kobylianskiĭ
A G
AG
Pal'keeva
M E
ME
Novikov
A A
AA
Reshetniak
T M
TM
Kliukvina
N G
NG
Nasonov
E L
EL
D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000276:immunology*; D016736:Antiphospholipid Syndrome / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D001666:Binding Sites, Antibody; D015415:Biomarkers / Q000097:blood; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood* / Q000276:immunology; D011379:Prognosis; D053482:beta 2-Glycoprotein I
D017153:Antibodies, Anticardiolipin; D015415:Biomarkers; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053482:beta 2-Glycoprotein I