Simultaneous detection of tyrosine hydroxylase-immunoreactivity and vasopressin mRNA in neurons of the human paraventricular and supraoptic nucleus.
Our purpose was to investigate the proportion of tyrosine hydroxylase (TH)-immunoreactive (IR) neurons expressing vasopressin (VP) mRNA in the human paraventricular and supraoptic nuclei by combining in situ hybridization with immunohistochemistry on the same tissue section. A variability in the proportion of TH-IR neurons synthesizing VP mRNA was observed in adults which was usually more than 50%. In neonates almost all the TH-IR neurons appeared to contain VP mRNA.
Panayotacopoulou
M T
MT
Department of Psychiatry, Eginition Hospital, University of Athens, 74 Vassilissis Sophias Ave., Athens, Greece. mpana@cc.uoa.gr
Goudsmit
E
E
Van Heerikhuize
J J
JJ
Swaab
D F
DF
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D005260:Female; D006801:Humans; D007177:Inappropriate ADH Syndrome / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D013495:Supraoptic Nucleus / Q000737:chemistry / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis* / Q000276:immunology; D014667:Vasopressins / Q000235:genetics*
D000906:Antibodies; D012333:RNA, Messenger; D014667:Vasopressins; D014446:Tyrosine 3-Monooxygenase