Analysis of mfbA alleles of the eubasidiomycete Lentinus edodes.
A fruiting-body-specific mfbA cDNA derived from Lentinus edodes FMC2 has been shown to encode a high-molecular-weight protein, MFBA, containing the cell-adhesion-promoting Arg-Gly-Asp (RGD) sequence. Southern-blot analysis showed that all L. edodes strains tested have the mfbA gene (homologue). Nucleotide sequence analysis of the 1-kb mfbA fragments containing the RGD-coding sequence showed that each L. edodes strain has two types of mfbA homologues. It was found in FMC2 that two mfbA homologues are derived from different nuclei and these mfbA alleles are transcribed with similar frequencies in the fruiting bodies.
Yasuda
T
T
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Shishido
K
K
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D005656:Fungal Proteins; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D020942:Shiitake Mushrooms / Q000235:genetics*
D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; C092229:MfbAC protein, Lentinula edodes; D010940:Plant Proteins