title: Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil.
abstract: Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent.
authors.affiliation: Departamento de Microbiologia e Parasitologia, CCB, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil. grisard@ccb.ufsc.br
authors.forename: E C
authors.initials: EC
authors.lastname: Grisard
authors.forename: M
authors.initials: M
authors.lastname: Steindel
authors.forename: J J
authors.initials: JJ
authors.lastname: Shaw
authors.forename: E A
authors.initials: EA
authors.lastname: Ishikawa
authors.forename: C J
authors.initials: CJ
authors.lastname: Carvalho-Pinto
authors.forename: I
authors.initials: I
authors.lastname: Eger-Mangrich
authors.forename: H K
authors.initials: HK
authors.lastname: Toma
authors.forename: J H
authors.initials: JH
authors.lastname: Lima
authors.forename: A J
authors.initials: AJ
authors.lastname: Romanha
authors.forename: D A
authors.initials: DA
authors.lastname: Campbell
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D001938:Brazil; D005091:Exons; D005819:Genetic Markers; D006801:Humans; D007892:Leishmania braziliensis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007894:Leishmania mexicana / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012424:Rural Population; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D005819:Genetic Markers