Establishment of a phagocytic cell line from Bombina orientalis.
Continuous serum-free culture of Bok-2 cells was generated from primary culture of the tail bud stage embryos of the toad, Bombina orientalis. Bok-2 cells can be maintained in modified L-15 serum-free medium prepared by mixing L-15 medium, lactalbumin enzymatic hydrolysate, sucrose and sodium bicarbonate. Bok-2 cells have an ameboid behavior and morphology. When Bok-2 and viable Candida albicans were co-cultured, Bok-2 cells showed an immune response characterized by chemotaxis, phagocytosis and partial clearing activity of Candida cells and colonies. And Bok-2 cells also showed phagocytosis of latex beads without serum treatment and displayed numerous pseudopodia, membrane evagination for phagocytosis and phagosome activity. On the basis of these results, Bok-2 cells were identified as professional phagocytes.
Park
S C
SC
Cell Biology Laboratory, Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
Lee
S H
SH
Han
S S
SS
D000818:Animals; D001001:Anura / Q000196:embryology / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002633:Chemotaxis; D016895:Culture Media, Serum-Free; D004625:Embryo, Nonmammalian / Q000166:cytology; D010586:Phagocytes / Q000166:cytology*
D016895:Culture Media, Serum-Free