title: Multiple single unit recording in the cortex of monkeys using independently moveable microelectrodes.
abstract: Simultaneous recording from multiple single neurones presents many technical difficulties. However, obtaining such data has many advantages, which make it highly worthwhile to overcome the technical problems. This report describes methods which we have developed to permit recordings in awake behaving monkeys using the 'Eckhorn' 16 electrode microdrive. Structural magnetic resonance images are collected to guide electrode placement. Head fixation is achieved using a specially designed headpiece, modified for the multiple electrode approach, and access to the cortex is provided via a novel recording chamber. Growth of scar tissue over the exposed dura mater is reduced using an anti-mitotic compound. Control of the microdrive is achieved by a computerised system which permits several experimenters to move different electrodes simultaneously, considerably reducing the load on an individual operator. Neurones are identified as pyramidal tract neurones by antidromic stimulation through chronically implanted electrodes; stimulus control is integrated into the computerised system. Finally, analysis of multiple single unit recordings requires accurate methods to correct for non-stationarity in unit firing. A novel technique for such correction is discussed.
authors.affiliation: Sobell Department of Neurophysiology, Institute of Neurology, London, UK. snb11@cam.ac.uk
authors.forename: S N
authors.initials: SN
authors.lastname: Baker
authors.forename: N
authors.initials: N
authors.lastname: Philbin
authors.forename: R
authors.initials: R
authors.lastname: Spinks
authors.forename: E M
authors.initials: EM
authors.lastname: Pinches
authors.forename: D M
authors.initials: DM
authors.lastname: Wolpert
authors.forename: D G
authors.initials: DG
authors.lastname: MacManus
authors.forename: Q
authors.initials: Q
authors.lastname: Pauluis
authors.forename: R N
authors.initials: RN
authors.lastname: Lemon
mesh_terms: D000818:Animals; D004567:Electrodes, Implanted; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D000882:Haplorhini / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D008839:Microelectrodes; D009044:Motor Cortex / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology*