title: Cell surface display of hepatitis B virus surface antigen by using Pseudomonas syringae ice nucleation protein.
abstract: A new system designed for cell surface display of recombinant proteins on Escherichia coli was evaluated for expression of eukaryotic viral antigens. The major surface antigen of hepatitis B virus (HBsAg) was fused to the ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae. Western blotting, immunofluorescence microscopy, whole-cell ELISA, and ice nucleation activity assay confirmed expression of recombinant proteins on the surface of Escherichia coli. This study indicated that INP-based cell surface display can be used for epitope mapping and recombinant bacteria expressing hepatitis viral antigens may be used for developing live vaccines.
authors.affiliation: Department of Biotechnology, College of Engineering and Bioproducts Research Center, Yonsei University, Seoul, Korea.
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
authors.forename: S K
authors.initials: SK
authors.lastname: Yoo
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010957:Plasmids; D011549:Pseudomonas / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006514:Hepatitis B Surface Antigens; D011993:Recombinant Fusion Proteins; C063113:ice nucleation protein