title: Real-time PCR-analysis of the cytochrome P450 1B1 codon 432-polymorphism.
abstract: The aim of the present study was to develop and validate a rapid assay for genotyping of CYP1B1 codon 432-polymorphism. The described method is a single tube assay and combines both rapid-cycle polymerase chain reaction (PCR) with real-time monitoring by amplification and generation of the melting profiles of an allele-specific fluorescent probe. With this method 300 samples were analysed from healthy, unrelated Germans. Genotype frequency determined for the mutated allele CYP1B1*2 was 0.40. The results show that genotyping of CYP1B1 codon 432-polymorphism with a real-time fluorescence PCR method is a rapid and reliable assay for the analysis of large numbers of samples.
authors.affiliation: Institut für Arbeitsphysiologie an der Universität Dortmund, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Brüning
authors.forename: J
authors.initials: J
authors.lastname: Abel
authors.forename: B
authors.initials: B
authors.lastname: Koch
authors.forename: K
authors.initials: K
authors.lastname: Lorenzen
authors.forename: V
authors.initials: V
authors.lastname: Harth
authors.forename: S
authors.initials: S
authors.lastname: Donat
authors.forename: A
authors.initials: A
authors.lastname: Sachinidis
authors.forename: H
authors.initials: H
authors.lastname: Vetter
authors.forename: H M
authors.initials: HM
authors.lastname: Bolt
authors.forename: Y
authors.initials: Y
authors.lastname: Ko
mesh_terms: D000483:Alleles; D001189:Aryl Hydrocarbon Hydroxylases; D003062:Codon / Q000235:genetics*; D065633:Cytochrome P-450 CYP1B1; D003577:Cytochrome P-450 Enzyme System / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics*; D013050:Spectrometry, Fluorescence
chemical_list: D003062:Codon; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1