Seasonal changes in sex and adrenal steroid hormones of gopher tortoises (Gopherus polyphemus).
We sampled a population of gopher tortoises (Gopherus polyphemus) from May to October 1997 to determine seasonal cycles of steroid hormones (testosterone, T; 17beta-estradiol, E; and progesterone, P) and related them to observations of mating behavior. In males, plasma T levels peaked in July and August and remained elevated through October. This coincides with the reported time of peak mating and spermatogenesis, indicating that males display an associated pattern of reproduction. In females, E levels were high in September and October. Plasma T levels in females were elevated in May, decreased to basal levels in June and July, and rose again in August and September. Elevated E and T levels correspond to the reported time of peak vitellogenic activity, indicating that females also display an associated cycle. Plasma P in females remained basal throughout the active season, suggesting that ovulation occurs in late winter. We also determined levels of corticosterone (B) to assess the influence of capture stress on tortoises and correlated B levels with tortoise activity patterns and sex steroid levels. We found no seasonal variation in levels of B in males or females. Plasma B levels were not correlated with levels of T or E, but were positively correlated with female P levels. Further, we found no relationship between plasma B levels in males and mean distance moved, mean number of burrows used, or mean home range size. However, there was a significant negative correlation between plasma B levels and male body size. In females, there was no relationship between B levels and mean distance moved, but B levels were significantly negatively correlated with the number of burrows females occupied. Lastly, there was no relationship between levels of B and the number of minutes required to obtain blood from an animal. However, B levels increased with the length of time that a tortoise spent in a trap, suggesting that trapped tortoises do exhibit capture stress.
Ott
J A
JA
Department of Zoology and Wildlife Science, Auburn University, Alabama 36849, USA. jott@jonesctr.org
Mendonça
M T
MT
Guyer
C
C
Michener
W K
WK
D000305:Adrenal Cortex Hormones / Q000097:blood*; D000818:Animals; D001835:Body Weight / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D004958:Estradiol / Q000097:blood; D005260:Female; D012739:Gonadal Steroid Hormones / Q000097:blood*; D006128:Growth / Q000502:physiology; D008297:Male; D009043:Motor Activity / Q000502:physiology; D006080:Ovarian Follicle / Q000502:physiology; D010053:Ovary / Q000254:growth & development / Q000502:physiology; D011374:Progesterone / Q000097:blood; D011863:Radioimmunoassay; D012621:Seasons; D013315:Stress, Psychological / Q000378:metabolism; D013737:Testis / Q000254:growth & development / Q000502:physiology; D013739:Testosterone / Q000097:blood; D014426:Turtles / Q000502:physiology*
D000305:Adrenal Cortex Hormones; D012739:Gonadal Steroid Hormones; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D003345:Corticosterone