Observation of soft contact lens disinfection with fluorescent metabolic stains.
A rapid fluorescent staining method using a tetrazolium dye and propidium iodide for the in-use assessment of disinfection of Pseudomonas aeruginosa biofilms on soft contact lenses showed that 11 to 13% of cells on lenses remained actively respiring and recoverable by culture methods after 30 min of exposure to 3% hydrogen peroxide.
Gavin
J
J
School of Biological and Biomedical Sciences, United Kingdom.
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Watson-Craik
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D018441:Biofilms / Q000187:drug effects; D004396:Coloring Agents; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology*; D004203:Disinfection / Q000379:methods*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D011419:Propidium; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013777:Tetrazoles; D013778:Tetrazolium Salts
D004396:Coloring Agents; D013777:Tetrazoles; D013778:Tetrazolium Salts; C075453:5-cyano-2,3-ditolyltetrazolium; D011419:Propidium; D006861:Hydrogen Peroxide