The entire nucleotide sequence of two hepatitis G virus isolates belonging to a novel genotype: isolation in Myanmar and Vietnam.
A novel genotype of hepatitis G virus (HGV) was recently identified in sera of subjects from countries in South-East Asia. These isolates were recovered from serum of Myanmarese (designated HGV-MY14) and Vietnamese (designated HGV-VT48) subjects, respectively. To characterize the viral genome in more detail, the full-length nucleotide sequence of the two different HGV isolates belonging to the novel genotype was cloned. Both HGV isolates were composed of 9228 nt and had a single open reading frame spanning 8529 nt and encoding 2843 aa residues. The isolates differed from previously reported HGV/GBV-C isolates types 1 to 3 by 13-15% (nucleotide sequence) and 4-6% (amino acid sequence). The putative core region of both isolates was not clearly identifiable as it consisted of only 16 aa residues. Based on phylogenetic analysis of full-length genome sequences and 5'-UTR sequences, HGV-MY14 and HGV-VT48 isolates can be classified as a novel genotype, designated type 4.
Naito
H
H
Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
Hayashi
S
S
Abe
K
K
D000293:Adolescent; D000328:Adult; D018076:DNA, Complementary; D005260:Female; D018067:Flaviviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016679:Genome, Viral; D005838:Genotype; D006525:Hepatitis, Viral, Human / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D002052:Myanmar; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood; D017422:Sequence Analysis, DNA; D014744:Vietnam
D018076:DNA, Complementary; D012367:RNA, Viral