Evaluation of a latex agglutination kit for the detection of human antibodies to the lipopolysaccharide of Escherichia coli O157, following infection with verocytotoxin-producing E. coli O157.
A total of 150 human sera was used to evaluate a commercial latex agglutination kit for detecting antibodies to the lipopolysaccharide of Escherichia coli O157. A comparison of the kit with SDS-PAGE and immunoblotting showed that the kit had a sensitivity of 94.12%, a specificity of 99.15%, a positive predictive value of 96.97% and a negative predictive value of 99.15%.
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Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK. hchart@phls.co.uk
D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000276:immunology* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D007231:Infant, Newborn; D007841:Latex Fixation Tests; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D022622:Shiga Toxin 1
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D008070:Lipopolysaccharides; D011933:Reagent Kits, Diagnostic; D022622:Shiga Toxin 1