Inhomogeneous alginate gel spheres: an assessment of the polymer gradients by synchrotron radiation-induced X-ray emission, magnetic resonance microimaging, and mathematical modeling.
It has been previously demonstrated that calcium alginate gels prepared by dialysis often exhibit a concentration inhomogeneity being the polymer concentration considerably lower in the center of the gel than at the edges. Inhomogeneity may be a preferred structure in microcapsules due to low porosity and higher stability so that it is interesting to evaluate the polymer gradient in spherically symmetrical small alginate beads (1.0-0.7 mm diameter) obtained in different conditions. In this paper, two complementary techniques have been used to investigate this aspect. The concentration gradient of alginate has been analyzed by measuring both the spatial distribution of calcium ions in sections of alginate gel spheres, by means of x-ray fluorescence spectroscopy, and the T2 relaxation behavior on intact gel beads using magnetic resonance microimaging. The experimentally determined gradients from three-dimensional gels provide data to reevaluate the parameter estimates in the recently reported mathematical model for alginate gel formation (A. Mikkaelsen and A. Elgsaeter, Biopolymers, 1995, Vol. 36, pp. 17-41). The model may account for the gels being less inhomogeneous when nongelling sodium or magnesium ions are added during gelation.
Thu
B
B
Institute of Biotechnology, NTNU, Trondheim, Norway.
Gåserød
O
O
Paus
D
D
Mikkelsen
A
A
Skjåk-Braek
G
G
Toffanin
R
R
Vittur
F
F
Rizzo
R
R
D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D004058:Diffusion; D005782:Gels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007700:Kinetics; D008279:Magnetic Resonance Imaging / Q000379:methods; D008863:Microspheres; D008956:Models, Chemical; D017356:Synchrotrons
D000464:Alginates; D001672:Biocompatible Materials; D005782:Gels; D006603:Hexuronic Acids; D020723:Glucuronic Acid