title: Oncoprotein coexpression in human aberrant crypt foci and minute polypoid lesions of the large bowel.
abstract: BACKGROUND
  Genetic aberrations observed in the large bowel during the neoplastic progression have a cumulative effect and are responsible for the propagation of the multistep malignant process. In the present study we evaluated the immunoreactivity of c-fos, ras, bcl-2 and p53 in aberrant crypt foci (ACF) and minute polyps of the large bowel obtained from patients with colorectal cancer.
  
  
  METHODS
  ACF and minute polyps were collected from macroscopically normal colonic mucosa. Protein immunoreactivity was detected on parafin sections utilizing the biotin-streptavidin method on 25 hyperplastic, 10 dysplastic ACF, 5 hyperplastic and 10 dysplastic adenomas.
  
  
  RESULTS
  41% of the lesions displayed positive ras immunoreactivity. bcl-2 immunoreactivity was positive in six minute polyps of which five were neoplastic. fos immunoreactivity was detected in five ACF and seven minute polyps, mainly in dysplastic lesions. Two neoplastic polyps were positive for p53 immunoreactivity. Coexpression of two or more oncoproteins was found with increasing frequency in dysplastic versus hyperplastic lesions and in polypoid lesions versus ACF.
  
  
  CONCLUSIONS
  Abnormal expression and coexpression in oncoproteins can be identified in the earliest stages of colorectal tumorigenesis and may contribute to the progression of selected lesions during ACF-adenoma-carcinoma sequence.
authors.affiliation: Department of Surgery B, Meir General Hospital, Sapir Medical Center, Kfar Sava, Israel.
authors.forename: B
authors.initials: B
authors.lastname: Shpitz
authors.forename: Y
authors.initials: Y
authors.lastname: Bomstein
authors.forename: M
authors.initials: M
authors.lastname: Shalev
authors.forename: S
authors.initials: S
authors.lastname: Liverant
authors.forename: Z
authors.initials: Z
authors.lastname: Kaufman
authors.forename: E
authors.initials: E
authors.lastname: Klein
authors.forename: Y
authors.initials: Y
authors.lastname: Mekori
authors.forename: J
authors.initials: J
authors.lastname: Bernheim
mesh_terms: D003106:Colon / Q000378:metabolism* / Q000473:pathology; D003111:Colonic Polyps / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D011123:Polyploidy