Suramin-sensitive suppression of paired-pulse inhibition by adenine nucleotides in rat hippocampal slices.
In order to assess the possible presence of presynaptic P2 receptors for nucleotides in the hippocampus, adenosine triphosphate and betagamma-methyleneATP have been examined on paired-pulse inhibition in rat hippocampal slices. Both compounds reproduced the effects of adenosine and reduced the amount of paired-pulse inhibition at an interpulse interval of 10 ms and increased the amount of facilitation at intervals of 20 and 50 ms. These effects were prevented by 8-phenyltheophylline and adenosine deaminase, indicating their mediation by adenosine. The effects were also reduced by suramin at 50 microM, suggesting the possible activation of P2 receptors. It is suggested that a population of P2 receptors may exist which promote the release of endogenous adenosine in the hippocampus.
Nikbakht
M R
MR
Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, UK.
Stone
T W
TW
D000200:Action Potentials / Q000187:drug effects; D000227:Adenine Nucleotides / Q000494:pharmacology*; D000241:Adenosine / Q000037:antagonists & inhibitors*; D000243:Adenosine Deaminase / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D006624:Hippocampus / Q000187:drug effects*; D008297:Male; D009419:Nerve Tissue Proteins / Q000187:drug effects*; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017661:Receptors, Presynaptic / Q000187:drug effects*; D018048:Receptors, Purinergic P2 / Q000187:drug effects*; D013498:Suramin / Q000494:pharmacology*; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
D000227:Adenine Nucleotides; D009419:Nerve Tissue Proteins; D017661:Receptors, Presynaptic; D018048:Receptors, Purinergic P2; C005147:5'-adenylyl (beta,gamma-methylene)diphosphonate; D013498:Suramin; D000255:Adenosine Triphosphate; D013806:Theophylline; C028322:8-phenyltheophylline; D000243:Adenosine Deaminase; D000241:Adenosine; C002630:alpha,beta-methyleneadenosine 5'-triphosphate