Involvement of superoxide and/or nitric oxide in renal tissue injury.
We examined the influence of superoxide (O2-) and/or nitric oxide (NO) on renal tissue injury estimated from the levels of lipid peroxidation and sulfhydryl (SH) oxidation. Pyrogallol, an O2- generator and precursor of hydrogen peroxide, produced marked tissue injury, but this was prevented by superoxide dismutase (SOD)/catalase (CAT). Hemoglobin (Hb), a NO scavenger, provided protection from tissue injury caused by sodium nitroprusside (SNP). The tissue injury produced by 3-morpholinosydnonimine (SIN-1), which is thought to form peroxynitrite (ONOO-) as a simultaneous O2- and NO generator, was blocked by SOD/CAT or Hb. On the other hand, protein-SH and nonprotein-SH were significantly increased by addition of SOD/CAT or Hb. These data suggest that the renal tissue injury induced by O2-, NO and ONOO- can be blocked by SH, suggesting an important protective role against these reactive oxygen species in the mechanism of tissue defense.
Yokozawa
T
T
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
Chung
H Y
HY
Kim
D W
DW
Goto
H
H
D000818:Animals; D007674:Kidney Diseases / Q000503:physiopathology*; D015227:Lipid Peroxidation / Q000502:physiology*; D051379:Mice; D009569:Nitric Oxide / Q000009:adverse effects* / Q000494:pharmacology; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013481:Superoxides / Q000009:adverse effects* / Q000494:pharmacology
D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D013481:Superoxides; D009569:Nitric Oxide