title: Matrix metalloproteinase-7-dependent release of tumor necrosis factor-alpha in a model of herniated disc resorption.
abstract: Herniated disc (HD), one of the major causes of low back pain, is often resolved spontaneously without surgical intervention. Resorption is associated with a marked increase in infiltrating macrophages, and the matrix metalloproteinases (MMP) MMP-3 and MMP-7 have been implicated in this phenomenon. We developed a murine organ culture model in which intact intervertebral discs were cocultured with peritoneal macrophages to investigate the role of MMPs in HD resorption. Using macrophages isolated from MMP-null mice, we report that macrophage-produced MMP-7 was required for proteoglycan degradation, loss of wet weight, and macrophage infiltration of cocultured discs. The inability of MMP-7-deficient macrophages to infiltrate discs could not be attributed to a defect in macrophage migration. MMP-7 was required for the release of the cytokine TNF-alpha from peritoneal macrophages. The generation of soluble TNF-alpha was essential for the induction of MMP-3 in disc cocultures, which in turn is required for the generation of a macrophage chemoattractant and subsequent macrophage infiltration. TNF-alpha release from macrophages was necessary but insufficient for disc resorption, which required macrophage infiltration. We conclude that there is extensive communication between macrophages and chondrocytes in HD resorption and that an essential component of this communication is the requirement for MMPs to release soluble bioactive factors.
authors.affiliation: Department of Orthopaedics and Rehabilitation, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.
authors.forename: H
authors.initials: H
authors.lastname: Haro
authors.forename: H C
authors.initials: HC
authors.lastname: Crawford
authors.forename: B
authors.initials: B
authors.lastname: Fingleton
authors.forename: K
authors.initials: K
authors.lastname: Shinomiya
authors.forename: D M
authors.initials: DM
authors.lastname: Spengler
authors.forename: L M
authors.initials: LM
authors.lastname: Matrisian
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000201:enzymology; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D018920:Coculture Techniques; D015194:Diffusion Chambers, Culture; D004195:Disease Models, Animal; D004790:Enzyme Induction; D007403:Intervertebral Disc / Q000166:cytology / Q000201:enzymology; D007405:Intervertebral Disc Displacement / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D019278:Matrix Metalloproteinase 3 / Q000096:biosynthesis; D020783:Matrix Metalloproteinase 7 / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D019278:Matrix Metalloproteinase 3; D020783:Matrix Metalloproteinase 7