Identification of a gene linked to the Brassica S (self-incompatibility) locus by differential display.
Self-incompatibility in Brassica is controlled by a complex locus, the S locus, that includes several expressed genes. Two S locus genes, SLG and SRK, are expressed in the stigma and have been implicated in self-pollen recognition. The male component of this recognition system is also predicted to be encoded by a gene at the S locus but this gene has not been identified to date. In this study, we have used differential display to screen for polymorphic, S-locus-linked genes that are expressed in anthers. This approach has allowed the identification of a gene, named S5J, which was shown to segregate completely with the S locus. We discuss the possible role of this gene in the self-incompatibility response and evaluate the utility of differential display for the identification of genes at specific genetic loci.
Miege
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Reproduction et développement des plantes, UMR 5667 CNRS-Inra-ENSL, Ecole normale supérieure de Lyon, France.
Dumas
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Cock
J M
JM
D001483:Base Sequence; D001937:Brassica / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D011058:Pollen / Q000378:metabolism; D011110:Polymorphism, Genetic; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D012098:Reproduction / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013382:Subtraction Technique
D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant