title: Diversity within the VP4 gene of rotavirus P[8] strains: implications for reverse transcription-PCR genotyping.
abstract: A degenerate version (1T1-D) of the rotavirus P[8]-specific primer (1T-1) allowed strains previously untypeable due to the accumulation of point mutations at the primer binding site to be P typed by reverse transcription-PCR. Sequencing of the cDNA followed by sequence alignment and phylogenetic analysis identified lineages and sublineages within the rotavirus P[8] types, while the use of 1T-1 or 1T-1D primers did not yield viral clusters in any particular lineage.
authors.affiliation: Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge CB2 2QW, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Iturriza-Gómara
authors.forename: J
authors.initials: J
authors.lastname: Green
authors.forename: D W
authors.initials: DW
authors.lastname: Brown
authors.forename: U
authors.initials: U
authors.lastname: Desselberger
authors.forename: J J
authors.initials: JJ
authors.lastname: Gray
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D017931:DNA Primers; D005814:Genes, Viral; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; C060339:VP4 protein, Rotavirus