[Cytoprotective effect of antihypoxic and antioxidant preparations on cultured human cells in a model of toxic response].
An oxidative stress is considered to be one of the major mechanisms of cytotoxicity. The purpose of present work was to study effects of some drugs with antihypoxic/antioxidant activity in cultured human lung embryonic fibroblasts under conditions of cytotoxic response, provoked by cationic or anionic antiseptics. The following preparations were under study: Mafusol (Na-fumarate), superoxide dismutase from human erythrocytes (SOD), cytochrome c, alpha-tocopherol and Thioctacid T (lipoate) which were applied at concentrations comparable with those, employed in clinical application. The combinations of the used drugs were also under study. The cytotoxic response was induced by an application of antiseptics into the cell incubation medium in 2-5 fold dilutions up to minimum toxic doses for 2-24 h. The drugs under study were introduced simultaneously with antiseptics. The maximum cytoprotective effect was revealed in the case of combination fumarate-alpha-tocopherol; the combination fumarate plus SOD being the second in effectiveness. When the drugs were introduced separately, the most effective proved to be fumarate, followed by vitamin E and cytochrome c. SOD and lipoate did not reveal any cytoprotective activity in our experimental conditions. The designed model of cytotoxicity in vitro can be considered as a prospective test-system for the screening of cytoprotective drugs and their combinations.
Eropkin
M Iu
MIu
Institute of Influenza, Russian Medical Academy, St. Petersburg.
Smirnova
T D
TD
Eropkina
E M
EM
Mamaeva
E G
EG
D000975:Antioxidants / Q000494:pharmacology*; D015687:Cell Hypoxia / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*
D000975:Antioxidants