title: Enhancement of progenitor cell division in the dentate gyrus triggered by initial limbic seizures in rat models of epilepsy.
abstract: OBJECTIVE
  Mitogenic effects of seizures on granule cell progenitors in the dentate gyrus were studied in two rat models of epilepsy. We investigated which stage of epileptogenesis is critical for eliciting progenitor cell division and whether seizure-induced neuronal degeneration is responsible for the enhancement of progenitor cell division.
  
  
  METHODS
  Seizures were induced by either kainic acid (KA) administration or electrical kindling. Neurogenesis of dentate granule cells was evaluated using the bromodeoxyuridine (BrdU) labeling method, and neuronal degeneration was assessed by in situ DNA fragmentation analysis.
  
  
  RESULTS
  After injection of KA, the number of BrdU-positive granule cells began to increase at day 3 after the treatment, peaked at day 5, and returned to baseline at day 10. By day 13, the values were lower than control. After kindling, the number of BrdU-positive cells began to increase after five consecutive experiences of stage I seizures. The increase occurred from day 1 to day 3 after the last electrical stimulation, but returned to baseline by day 7. After generalized seizures were well established, repeated stimulation did not facilitate division of granule cell progenitors. DNA fragmentation was noted in pyramidal neurons in the CA1, CA3, and hilus regions at 18 h after KA injection, but not in the kindling model.
  
  
  CONCLUSIONS
  These observations indicate that a mechanism in epileptogenesis boosts dentate progenitor cell division, but progenitor cells may become unreactive to prolonged generalized seizures. Pyramidal neuronal degeneration is not necessary for triggering the upregulation. It is suggested that newly born granule cells may play a role in the network reorganization that occurs during epileptogenesis.
authors.affiliation: Molecular Neuroscience Research Center, and Department of Pediatrics, Shiga University of Medical Science, Otsu, Japan. eijin@interchange.ubc.ca
authors.forename: E
authors.initials: E
authors.lastname: Nakagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Aimi
authors.forename: O
authors.initials: O
authors.lastname: Yasuhara
authors.forename: I
authors.initials: I
authors.lastname: Tooyama
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: P L
authors.initials: PL
authors.lastname: McGeer
authors.forename: H
authors.initials: H
authors.lastname: Kimura
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D053938:DNA Fragmentation; D018891:Dentate Gyrus / Q000473:pathology*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008032:Limbic System / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000473:pathology*; D013234:Stem Cells / Q000473:pathology*; D013997:Time Factors
chemical_list: D001973:Bromodeoxyuridine