title: Ileal lipid infusion stimulates jejunal synthesis of apolipoprotein A-IV without affecting mRNA levels.
abstract: We examined the effect of ileal infusions of lipid emulsion on mRNA levels and biosynthesis of apolipoprotein A-IV (apo A-IV) in jejunal Thiry-Vella fistulas in rats. The rats were surgically prepared with jejunal Thiry-Vella fistulas; after recovery they were deprived of food, equipped with ileal infusion cannulas, then given 8 hr ileal infusions of fatty acid/monoglyceride emulsions. Mucosal synthesis and transcript levels of apo A-IV in the Thiry-Vella loop were then measured. Lipid infusion produced a two-fold stimulation in incorporation of 3H-leucine into apo A-IV-specific protein, but had no significant effect on apo A-IV mRNA levels. These results support the hypothesis that a lipid-elicited, distal gut-derived, systemic signal stimulates the production of apo A-IV by a post-transcriptional mechanism.
authors.affiliation: Department of Surgery, Louisiana State University Medical Center, Shreveport 71130, USA. tkalog@lsumc.edu
authors.forename: T J
authors.initials: TJ
authors.lastname: Kalogeris
authors.forename: R G
authors.initials: RG
authors.lastname: Painter
authors.forename: V R
authors.initials: VR
authors.lastname: Holden
mesh_terms: D000818:Animals; D001054:Apolipoproteins A / Q000096:biosynthesis* / Q000235:genetics; D005989:Glycerides / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism*; D050356:Lipid Metabolism; D008297:Male; D019301:Oleic Acid / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001054:Apolipoproteins A; D005989:Glycerides; D012333:RNA, Messenger; C022205:apolipoprotein A-IV; D019301:Oleic Acid; C005953:monoolein