Transcriptional analysis of a superoxide dismutase gene of Borrelia burgdorferi.
A single superoxide dismutase (Sod) gene was identified in Borrelia burgdorferi strains, Borrelia afzelii Ple and Borrelia garinii Pbi. Recombinant enzymatic activity was detected only when sod expression was controlled by the lacZ promoter in the cloning vector. Northern blot analysis with sod- or secA-specific probes identified a common 3.7-kb transcript. Reverse transcriptase-PCR analysis confirmed that secA and sod constitute a single transcriptional unit in B. burgdorferi. A transcriptional start site of this operon, containing -10 and -35 regions of a sigma(70)-type promoter, was mapped to 100 bp upstream of the ATG start codon of secA.
Nichols
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Department of Microbiology and Immunology, University of Louisville, School of Medicine, Louisville, KY, USA.
Whitehouse
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Austin
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D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015748:Borrelia burgdorferi Group / Q000201:enzymology* / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000382:microbiology; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D013482:Superoxide Dismutase; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins