The characteristics of peritoneal healing after catheter insertion in a rat model of peritoneal dialysis.
The aim of this study was to characterize the process of peritoneal healing after catheter insertion in a rat model of peritoneal dialysis using a 1.36% solution (Baxter Dianeal). After catheter insertion (day 1 of experiment), 12 rats were injected daily with 20 mL of 1.36% dialysis solution for 2 weeks. Every second day a sample of 4 hour dialysate was taken from every rat for biochemical analysis, cell count, cell differentiation, and nitrites measurement. The measured parameters decreased during the experiment and stabilized during the second week after catheter insertion. In cell differentiation, we observed an increase in the number of macrophages, with a parallel decrease in the number of neutrophils that was reflected in a significant decrease in the neutrophils/macrophages ratio. Our results suggest that the process of peritoneal healing after catheter implantation in rats lasts about 2 weeks, and therefore that any biocompatibility study of peritoneal dialysis solutions in rats should be performed at least 2 weeks after catheter insertion.
Wieczorowska-Tobis
K
K
Pathophysiology Department, University Medical School, Poznan, Poland.
Polubinska
A
A
Kuzlan-Pawlaczyk
M
M
Pawlaczyk
K
K
Breborowicz
A
A
Oreopoulos
D G
DG
D000818:Animals; D002408:Catheters, Indwelling / Q000009:adverse effects*; D002452:Cell Count; D015314:Dialysis Solutions / Q000737:chemistry; D017737:Macrophages, Peritoneal / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology; D009573:Nitrites / Q000032:analysis; D010530:Peritoneal Dialysis / Q000009:adverse effects / Q000295:instrumentation*; D010537:Peritoneum / Q000473:pathology* / Q000502:physiology; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014945:Wound Healing
D015314:Dialysis Solutions; D009573:Nitrites