Comparative evaluation of two branched-DNA human immunodeficiency virus type 1 RNA quantification assays with lower detection limits of 50 and 500 copies per milliliter.
We have comparatively evaluated Quantiplex version 3.0 and version 2. 0 on 133 plasma samples and a repetitive dilution series. Version 3. 0 yielded higher human immunodeficiency virus RNA values, and the ratio of version 3.0 results to version 2.0 results decreased from 3. 47 below 1,000 copies/ml to 1.97 above 50,000 copies/ml [linear regression, log (version 3.0) = 0.915 + 0.871 x log (version 2.0); r(2) = 0.952].
Manegold
C
C
Klinik für Gastroenterologie, Hepatologie und Infektiologie, Heinrich-Heine-Universität, Düsseldorf, Germany. manegold@bni.uni-hamburg.de
Krempe
C
C
Jablonowski
H
H
Kajala
L
L
Dietrich
M
M
Adams
O
O
D015342:DNA Probes; D005069:Evaluation Studies as Topic; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D019562:Viral Load
D015342:DNA Probes; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic