Constitutive NF-kappa B activity is modulated via neuron-astroglia interaction.
NF-kappa B is found in many neuronal cell types in different states of activity. This study aimed to define which conditions induce constitutive NF-kappa B activity in cultured hippocampal neurons using activity-specific antibody staining. In co-culture with astroglia, hippocampal neurons were devoid of activated NF-kappa B. In these co-cultures, NF-kappa B could not be activated via kainate or glutamate. In contrast, separating neurons from the glial compartment resulted in a time-dependent increase of activated neuronal NF-kappa B. In this line, activation of NF-kappa B by kainate or glutamate is very effective in freshly separated cultures, but inhibited when the cultures are reassembled after stimulation. These findings suggests that a neuronal-glial interaction may regulate gene expression via NF-kappa B.
Kaltschmidt
B
B
Molecular Neurobiology Laboratory, Albert-Ludwigs-University, Freiburg, Germany. kaltschm@uni-freiburg.de
Kaltschmidt
C
C
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian; D018698:Glutamic Acid / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D007608:Kainic Acid / Q000494:pharmacology; D016328:NF-kappa B / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats
D016328:NF-kappa B; D018698:Glutamic Acid; D007608:Kainic Acid