Both locus control region and proximal regulatory elements direct the developmental regulation of beta-globin gene cluster.
Using ligation-mediated PCR and in vivo footprinting methods to study the status of DNA-protein interaction at hypersensitive site 2 of locus control region and beta(maj) promoter of erythroid cells of fetal liver and adult bone marrow, we found that during different developmental periods, the status of DNA-protein interaction at both hypersensitive site 2 and beta(maj) promoter changed significantly, and indicated that locus control region might function through a looping mechanism to regulate the expression of downstream genes, and that distal regulatory elements (locus control region, hypersensitive sites) as well as proximal regulatory elements (promoter, enhancer) of beta-globin gene cluster participate in the regulation of developmental specificity.
Ji
X j
Xj
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Faculty of Basic Medicine, Peking Union Medical College, Beijing 100005, People's Republic of China.
Liu
D p
Dp
Xu
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Dd
Li
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L
Wang
J
J
Liang
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Cc
D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism; D004247:DNA / Q000235:genetics; D018983:DNA Footprinting; D005260:Female; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005809:Genes, Regulator; D005914:Globins / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D019901:Locus Control Region; D008297:Male; D051379:Mice; D005810:Multigene Family; D011247:Pregnancy; D011401:Promoter Regions, Genetic
D005914:Globins; D004247:DNA