Developmental regulation of the distribution of aromatase- and estrogen-receptor- mRNA-expressing cells in the zebra finch brain.
Strong evidence exists for the masculinizing effects of estrogen on the neural network that controls song learning and behavior in zebra finches. However, the mechanisms by which estrogen acts to influence the development of this circuitry are not well understood. In this study, we used in situ hybridization to detect the distribution of cells expressing mRNAs for AROM and ERalpha at postnatal days 5-25 (P5-25). Our findings revealed developmental regulation of both mRNAs in the neostriatum, archistriatum, hippocampus, diencephalon and midbrain. Within the vocal control circuitry, cells expressing ERalpha mRNA were found in the medial HVC (P10-25), archistriatum lateral to the RA (Ad; P25), in the ICo (P5-25), and along the fiber tract containing efferents from the RA. High levels of AROM mRNA were found in the neostriatum, including both the lateral and mMAN and along their projections to the RA and HVC, respectively, (P5-25), in the archistriatum (P18-25) and around RA (P18). Codistribution of the two mRNAs occurred along the border of the HVC suggesting that in this region, local synthesis of estrogen may be acting through its nuclear receptor to regulate gene transcription. Taken together, our findings show that the neural circuitry controlling song may be exposed to the effects of estrogen during early postnatal development. However, in most of the song control regions, these mRNAs were not expressed together either temporally or spatially, indicating that AROM may have a role in the development of the song system independent of ERalpha.
Jacobs
E C
EC
Program in Neuroscience, University of California, Los Angeles, CA, USA.
Arnold
A P
AP
Campagnoni
A T
AT
D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001921:Brain / Q000196:embryology* / Q000201:enzymology*; D001923:Brain Chemistry; D004027:Diencephalon / Q000737:chemistry / Q000201:enzymology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000737:chemistry / Q000201:enzymology; D017403:In Situ Hybridization; D008297:Male; D008636:Mesencephalon / Q000737:chemistry / Q000201:enzymology; D008969:Molecular Sequence Data; D011149:Pons / Q000737:chemistry / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D011960:Receptors, Estrogen / Q000235:genetics*; D020308:Songbirds; D014828:Vocalization, Animal / Q000502:physiology
D012333:RNA, Messenger; D011960:Receptors, Estrogen; D001141:Aromatase