Structure of a glycerol teichoic acid-like O-specific polysaccharide of Proteus vulgaris O12.
A phosphorylated O-specific polysaccharide (O-antigen) was obtained by mild acid degradation of Proteus vulgaris O12 lipopolysaccharide and studied by sugar and methylation analyses, 1H-, 13C- and 31P-NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, H-detected 1H, 13C and 1H, 31P heteronuclear multiple-quantum coherence experiments. It was found that the polysaccharide consists of pentasaccharide repeating units connected via a glycerol phosphate group, and has the following structure: where FucNAc is 2-acetamido-2,6-dideoxygalactose and the degree of O-acetylation at position 4 of GalNAc is approximately 25%. Immunochemical studies with P. vulgaris O12 O-antiserum suggested that the lipopolysaccharide studied shares common epitopes with the lipopolysaccharide core of P. vulgaris O8 and with the O-antigens of P. penneri strains 8 and 63.
Perepelov
A V
AV
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Torzewska
A
A
Shashkov
A S
AS
Senchenkova
S N
SN
Rozalski
A
A
Knirel
Y A
YA
D000818:Animals; D000907:Antibodies, Bacterial; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D005990:Glycerol / Q000737:chemistry; D007120:Immunochemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011511:Proteus / Q000737:chemistry / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry* / Q000276:immunology*; D011817:Rabbits; D013045:Species Specificity; D013682:Teichoic Acids / Q000737:chemistry
D000907:Antibodies, Bacterial; D019081:O Antigens; D013682:Teichoic Acids; D005990:Glycerol