title: Chimeras in noncoding regions between serotypes I and II of segment A of infectious bursal disease virus are viable and show pathogenic phenotype in chickens.
abstract: Two serotypes, I and II, have been identified for infectious bursal disease virus (IBDV), a member of the family BIRNAVIRIDAE: Here, the generation by reverse genetics of IBDV chimeras in segment A of the bisegmented genome is reported. The 5- and 3'-noncoding regions (NCRs) of a serotype II strain were exchanged with the NCRs of a full-length cDNA clone of segment A of a serotype I strain. Isolated chimeric viruses were characterized in cell culture and susceptible chickens. The results show that IBDV chimeras in segment A were able to replicate in cell culture and that VP1 encoded by a serotype I segment B is functionally active with serotype I NCRs as well as with serotype II NCRs. Chimeric viruses infected susceptible chickens and caused mild depletion of bursal cells. Thus, the noncoding regions of segment A are not responsible for the different pathotypes of IBDV serotypes I and II.
authors.affiliation: Institute of Molecular Biology, Friedrich-Loeffler-Institutes, Federal Research Centre for Virus Diseases of Animals, D-17498 Insel Riems, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schröder
authors.forename: A A
authors.initials: AA
authors.lastname: van Loon
authors.forename: D
authors.initials: D
authors.lastname: Goovaerts
authors.forename: E
authors.initials: E
authors.lastname: Mundt
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000209:etiology / Q000662:veterinary* / Q000821:virology; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D002678:Chimera / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D007243:Infectious bursal disease virus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010641:Phenotype; D011201:Poultry Diseases / Q000209:etiology* / Q000473:pathology / Q000821:virology; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D014774:Virulence / Q000235:genetics; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral