Covalent linkage of polyamines to peptidoglycan in Anaerovibrio lipolytica.
Spermidine and cadaverine were found to be constituents of the cell wall peptidoglycan of Anaerovibrio lipolytica, a strictly anaerobic bacterium. The peptidoglycan was degraded with the N-acetylmuramyl-L-alanine amidase and endopeptidase into two peptide fragments, peptide I and peptide II, at a molar ratio of 4:1. Peptides I and II were identified as L-alanine-D-glutamic acid(alphacadaverine)gammameso-diaminopimelic acid (DAP)-D-alanine and L-alanine-D-glutamic acid(alphaspermidine)gammameso-DAP-D-alanine, respectively. The N(1)-amino group of spermidine was linked to the alpha-carboxyl group of the D-glutamic acid residue of peptide II.
Hirao
T
T
Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Amamiya-machi, Aoba-ku, Sendai 981-8555, Japan.
Sato
M
M
Shirahata
A
A
Kamio
Y
Y
D002103:Cadaverine / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D002845:Chromatography / Q000379:methods; D002854:Chromatography, Paper; D006089:Gram-Negative Anaerobic Bacteria / Q000737:chemistry* / Q000378:metabolism; D010457:Peptidoglycan / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D011073:Polyamines / Q000032:analysis / Q000737:chemistry*; D013095:Spermidine / Q000737:chemistry
D010457:Peptidoglycan; D011073:Polyamines; D002103:Cadaverine; D013095:Spermidine