Transcriptional regulation of the POU gene Oct-6 in Schwann cells.
Genetic evidence suggests that the POU transcription factor Oct-6 plays a pivotal role as an intracellular regulator of Schwann cell differentiation. In the absence of Oct-6 function Schwann cells are generated in appropriate numbers and these cells differentiate normally up to the promyelin stage at which they transiently arrest. During peripheral nerve development Oct-6 expression is initiated in Schwann cell precursors and is strongly upregulated in promyelin cells. Oct-6 expression is subsequently extinguished in terminally differentiating Schwann cells. Thus, identification and characterisation of the DNA elements involved in this stage specific regulation may lead us to the signaling cascade and the axon-derived signals that drive Schwann cell differentiation and initiate myelination. Here we present experiments that aim at identifying such regulatory sequences.
Mandemakers
W
W
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, Netherlands.
Zwart
R
R
Kraay
R
R
Grosveld
G
G
Jaegle
A G
AG
Broos
L
L
Meijer
D
D
D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D050816:Octamer Transcription Factor-6; D012045:Regulatory Sequences, Nucleic Acid; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
D009419:Nerve Tissue Proteins; C494180:Pou3f1 protein, mouse; D014157:Transcription Factors; D050816:Octamer Transcription Factor-6; D001616:beta-Galactosidase