A positive selection vector combining tetracycline resistance that eliminates the need for bacterial plating.
We describe here the construction of a plasmid that combines positive selection with tetracycline resistance. The vector comprises a modified version of the gene encoding the cytosine-specific DNA methyltransferase MspI and a modified form of the pBR322 tetA(C) gene. The combination of these two genes facilitates the selection of recombinant plasmids in broth cultures, thereby eliminating the need for bacterial plating.
Matin
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MM
The Transgenomic Research Laboratory, University of Sheffield, Western Bank, S10 2TN, United Kingdom.
Hornby
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DP
D001431:Bacteriological Techniques; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D012641:Selection, Genetic; D013753:Tetracycline Resistance