The induction and potentiation of c-Jun and c-Fos expression in spinal neurons mediated by NK1 and NK2 receptors.
Specific antagonists were used to determine NK1 and NK2 receptor mediation of the expression of c-Jun and c-Fos inducible transcription factors. (ITFs) in dorsal horn neurons. The induction of c-Jun by C-fiber stimulation was strongly reduced in the superficial laminae by NK1 and NK2 antagonists, but only weakly in the deep laminae by NK2 antagonism. c-Fos induction was reduced in all laminae by both NK1 and NK2 antagonism but less than with c-Jun. The potentiation of c-Jun expression, caused by a preceding stimulus, was abolished in all laminae by NK1 and NK2 antagonists, whereas that of c-Fos was reduced in all laminae but again less than that of c-Jun. Thus, the expressions of ITFs mediated by NK1 and NK2 receptors are complex, depending upon the neurons and stimulus considered, and they are not a measure of the neurons' electrophysiological responsiveness.
Redburn
J L
JL
School of Biomolecular and Biomedical Sciences, Griffith University, Griffith, 4111, Australia.
Leah
J D
JD
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000494:pharmacology; D008297:Male; D064729:Neurokinin-1 Receptor Antagonists; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010456:Peptides, Cyclic / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D018040:Receptors, Neurokinin-1 / Q000502:physiology*; D018041:Receptors, Neurokinin-2 / Q000037:antagonists & inhibitors / Q000502:physiology*; D012584:Sciatic Nerve / Q000502:physiology; D013116:Spinal Cord / Q000502:physiology*
D001549:Benzamides; D001713:Biphenyl Compounds; D064729:Neurokinin-1 Receptor Antagonists; D010456:Peptides, Cyclic; D010880:Piperidines; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; C090951:MEN 10627; C073839:SR 48968; C066892:CP 96345