title: The ets protein PEA3 suppresses HER-2/neu overexpression and inhibits tumorigenesis.
abstract: Because HER-2/neu overexpression is important in cancer development, we looked for a method of suppressing the cell transformation mediated by HER-2/neu overexpression. We have identified that the DNA-binding protein PEA3, which is encoded by a previously isolated gene of the ets family, specifically targeted a DNA sequence on the HER-2/neu promoter and downregulated the promoter activity. Expression of PEA3 resulted in preferential inhibition of cell growth and tumor development of HER-2/neu-overexpressing cancer cells. This is a new approach to targeting HER-2/neu overexpression and also provides a rationale to the design for repressors of diseases caused by overexpression of pathogenic genes.
authors.affiliation: The University of Texas M.D. Anderson Cancer Center, Department of Cancer Biology, Section of Molecular Cell Biology, Houston, Texas 77030, USA.
authors.forename: X
authors.initials: X
authors.lastname: Xing
authors.forename: S C
authors.initials: SC
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Xia
authors.forename: Y
authors.initials: Y
authors.lastname: Zou
authors.forename: R
authors.initials: R
authors.lastname: Shao
authors.forename: K Y
authors.initials: KY
authors.lastname: Kwong
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Miller
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: M C
authors.initials: MC
authors.lastname: Hung
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D002471:Cell Transformation, Neoplastic; D017931:DNA Primers; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000473:pathology; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*
chemical_list: D017931:DNA Primers; D018719:Erb-b2 Receptor Tyrosine Kinases; D014157:Transcription Factors; C065788:transcription factor PEA3