title: Complementary mating types of Mucor circinelloides show electrophoretic karyotype heterogeneity.
abstract: Electrophoretic karyotypes of ten strains of Mucor circinelloides f. lusitanicus were generated by contour-clamped homogeneous electric field (CHEF) gel-electrophoresis. Most of the strains analyzed showed polymorphisms, but a different main karyotype pattern could be correlated with each mating type. Genome structure was further analyzed by gene assignment to the chromosome-sized DNAs. Nonradioactive hybridization techniques identified the chromosomal localization of seven cloned genes. The hybridization patterns confirmed the similarity between the mating-type (-) strains and showed some heterogeneity among the mating-type (+) strains. Linkage was found between genes pyrF and chs3 in all the strains, between the gene leuA and the rDNA in all mating-type (-) strains and ATCC 1216b (+), and between chs2 and the rDNA in CBS 969.68 (+). This is the first time that gene linkage in M. circinelloides has been reported, but in some cases the linkage relationships obtained are strain-dependent.
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Spain. josediaz@gugu.usal.es
authors.forename: J M
authors.initials: JM
authors.lastname: Díaz-Mínguez
authors.forename: M A
authors.initials: MA
authors.lastname: López-Matas
authors.forename: A P
authors.initials: AP
authors.lastname: Eslava
mesh_terms: D015139:Blotting, Southern; D015825:Chromosomes, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005816:Genetic Complementation Test; D018740:Genetic Heterogeneity; D008040:Genetic Linkage; D009089:Mucor / Q000737:chemistry* / Q000235:genetics*; D011110:Polymorphism, Genetic; D012098:Reproduction
chemical_list: D004275:DNA, Ribosomal