title: Evaluation of the database on mutant frequencies and DNA sequence alterations of vermilion mutations induced in germ cells of Drosophila shows the importance of a neutral mutation detection system.
abstract: The vermilion gene in Drosophila has extensively been used for the molecular analysis of mutations induced by chemicals in germ cells in vivo. The gene is located on the X-chromosome and is a useful target for the study of mutagenesis since all types of mutations are generated. We have critically evaluated this system with respect to sensitivity for mutation induction and selectivity for different types of mutations, using a database of more than 600 vermilion mutants induced in postmeiotic male germ cells by 18 mutagens. From most of these mutants the mutation has been analysed. These data showed 336 base substitutions, 96 intra-locus DNA rearrangements and 78 multi-locus deletions (MLD). Mutants containing a MLD were either heterozygous sterile or homozygous and hemizygous lethal. The distribution of both basepair (bp) changes and intra-locus rearrangements over the coding region of the vermilion gene was uniform with no preferences concerning 5' or 3' regions, certain exons, splice sites, specific amino acid changes or nonsense mutations. Possible hotspots for base substitutions seem to be related to the type of DNA damage rather than to the vermilion system. Gene mutations other than bp changes were examined on sequence characteristics flanking the deletion breakpoints. Induction frequencies of vermilion mosaic mutants were, in general, higher than those of vermilion complete mutants, suggesting that persistent lesions are the main contributors to the molecular spectra. Comparison of induction frequencies of vermilion mutants and sex-linked recessive lethal (SLRL) mutants for the 18 mutagens showed that the sensitivity of the vermilion gene against a mutagenic insult is representative for genes located on the X-chromosome. The effect of nucleotide excision repair (NER) on the formation of SLRL mutants correlated with an increase of transversions in the vermilion spectra under NER deficient conditions. Furthermore, the clastogenic potency of the mutagens, i.e., the efficiency to induce chromosomal-losses vs. SLRL forward mutations, shows a positive correlation with the percentage of DNA deletions in the molecular spectra of vermilion mutants.
authors.affiliation: Medical Genetics Centre South-West Netherlands (MGC), Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Centre, The Netherlands. nivard@rullf2.medfac.leidenuniv.nl
authors.forename: M J
authors.initials: MJ
authors.lastname: Nivard
authors.forename: I
authors.initials: I
authors.lastname: Aguirrezabalaga
authors.forename: L A
authors.initials: LA
authors.lastname: Ballering
authors.forename: A
authors.initials: A
authors.lastname: Pastink
authors.forename: L M
authors.initials: LM
authors.lastname: Sierra
authors.forename: E W
authors.initials: EW
authors.lastname: Vogel
mesh_terms: D000818:Animals; D004247:DNA / Q000187:drug effects; D016208:Databases, Factual; D004330:Drosophila / Q000187:drug effects / Q000235:genetics*; D029721:Drosophila Proteins; D005136:Eye Proteins; D015321:Gene Rearrangement; D005821:Genetic Techniques; D019476:Insect Proteins / Q000187:drug effects / Q000235:genetics*; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D014366:Tryptophan Oxygenase
chemical_list: D029721:Drosophila Proteins; D005136:Eye Proteins; D019476:Insect Proteins; D009153:Mutagens; D004247:DNA; D014366:Tryptophan Oxygenase; C121974:v protein, Drosophila