Decreased graft-versus-host disease after haplotype mismatched bone marrow allografts in miniature swine following interleukin-2 treatment.
Graft-versus-host disease (GVHD) is an important complication of bone marrow transplantation after transplants between HLA-mismatched donor/recipient pairs. In mice, giving IL-2 post transplant decreases GVHD in this setting. We studied high-dose IL-2 therapy in pigs. Transplants were carried out after conditioning with fractionated total body radiation and cyclophosphamide. Fourteen pigs received a fully mismatched bone marrow transplant (six with IL-2; eight without IL-2), and six received a single haplotype class II mismatched transplant (three with IL-2; three without IL-2). GVHD was evaluated by skin histology. All fully mismatched recipients had severe GVHD (grade 2-3) and died within 13 to 51 days whether or not they received IL-2. Pigs receiving a one haplotype class II mismatched transplant without IL-2 developed severe skin GVHD lasting for 8-45 days; all died within 57 days. Similar pigs receiving IL-2 post transplant had no or only mild skin GVHD for less than 15 days; two are long-term survivors. Bone Marrow Transplantation (2000) 25, 47-52.
Kozlowski
T
T
Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, Massachusetts 02129, USA.
Sablinski
T
T
Basker
M
M
Kitamura
H
H
Spitzer
T R
TR
Fishman
J
J
Sykes
M
M
Cooper
D K
DK
Sachs
D H
DH
D000818:Animals; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D006086:Graft vs Host Disease / Q000209:etiology / Q000517:prevention & control*; D006239:Haplotypes; D006650:Histocompatibility Testing; D007376:Interleukin-2 / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D013552:Swine; D013556:Swine, Miniature; D014184:Transplantation, Homologous
D007376:Interleukin-2