Effect of tumor necrosis factor-alpha and interleukin-2 on spleen lymphocyte migration in mouse skin.
Tumor necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2) are reported to enhance lymphocyte binding to endothelial cells in vitro. We examined these two agents on lymphocyte migration in vivo. Spleen lymphocytes were radiolabeled with tritiated uridine (3H-UR) and then injected i.v. into mice. Each cytokine (TNF-alpha or IL-2) or both cytokines were then injected intradermally on the back of mice. The results demonstrated that TNF-alpha stimulates lymphocyte migration in vivo in dose-dependent fashion. Kinetic analysis demonstrated that migration with TNF-alpha started at 3 h, peaked at 6 h, followed by a gradual decline back to baseline at 24 h. IL-2, on the other hand, was nearly inactive, and did not augment lymphocyte migration over and above that induced by TNF-alpha when both cytokines were injected together.
Sun
J
J
Department of Anatomy, Shandong Medical University, Jinan, People's Republic of China.
Liu
Z
Z
Bi
Y
Y
Guo
Z
Z
Hua
T
T
Ding
Z
Z
D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology*; D014316:Tritium; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000494:pharmacology*; D014529:Uridine
D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D014316:Tritium; D014529:Uridine