The trichothecene biosynthesis regulatory gene from the type B producer Fusarium strains: sequence of Tri6 and its expression in Escherichia coli.
A genomic DNA fragment containing Tri6, a transcription activator gene of trichothecene biosynthesis, was cloned by vectorette PCR from Fusarium graminearum F15, which produces type B trichothecene, deoxynivalenol. The nucleotide sequence of the gene showed 84% of identity to that of the type A trichothecene producer Fusarium sporotrichioides NRRL 3299, but the sequence around the initiation codon was not highly conserved between these producers. Based on the upstream and downstream sequences of the coding region of F. graminearum, Tri6 could be amplified by PCR from other type B trichothecene producers. Tri6 appeared to be expressed for only a limited period prior to the toxin production.
Matsumoto
G
G
Microbial Toxicology Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama, Japan.
Wuchiyama
J
J
Shingu
Y
Y
Kimura
M
M
Yoneyama
K
K
Yamaguchi
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli; D005656:Fungal Proteins; D005670:Fusarium / Q000235:genetics*; D005800:Genes, Fungal; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D014255:Trichothecenes / Q000096:biosynthesis*
D005656:Fungal Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C094327:Tri6 protein, Fusarium sporotrichioides; D014255:Trichothecenes