Fibroblast quiescence and the disruption of ERK signaling in mechanically unloaded collagen matrices.
Fibroblasts in mechanically unloaded collagen matrices had low levels of DNA synthesis compared with cells in mechanically loaded matrices. Under the former conditions, the cellular ERK signaling pathway appeared to be disrupted. Also, pharmacologic inhibition of ERK signaling blocked DNA synthesis by fibroblasts in mechanically loaded matrices. These results were consistent with the idea that mechanoregulation of fibroblast DNA synthesis in collagen matrices occurs at the level of the ERK signaling pathway.
Rosenfeldt
H
H
Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9039, USA.
Grinnell
F
F
D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020935:MAP Kinase Signaling System; D015398:Signal Transduction
D003094:Collagen; D017871:Calcium-Calmodulin-Dependent Protein Kinases