title: Intranasal immunization with Toxoplasma gondii SAG1 induces protective cells into both NALT and GALT compartments.
abstract: Intranasal (i.n.) immunization with the SAG1 protein of Toxoplasma gondii plus cholera toxin (CT) provides protective immunity. The aim of this study was to analyze the cellular activation of several mucosal compartments after i.n. immunization. Cervical and mesenteric lymph node (CLN and MLN, respectively) lymphoid cell and intraepithelial lymphocyte (IEL) passive transfer experiments were performed with CBA/J mice immunized i.n. with SAG1 plus CT. CLN and MLN cells and IEL isolated 42 days after immunization conferred protective immunity on naive recipient mice challenged with strain 76K T. gondii, as assessed by the reduction in the number of brain cysts. There were proliferative specific responses in nose-associated lymphoid tissue and the CLN and MLN cells from mice immunized with SAG1 plus CT, but no cytokine was detectable. Thus, protective immunity is associated with a specific cellular response in the nasal and mesenteric compartments after i.n. immunization.
authors.affiliation: Equipe Associée INRA d'Immunologie Parasitaire-EA 2637, UFR des Sciences Pharmaceutiques, 37200 Tours, France. velge@univ-tours.fr
authors.forename: F
authors.initials: F
authors.lastname: Velge-Roussel
authors.forename: P
authors.initials: P
authors.lastname: Marcelo
authors.forename: A C
authors.initials: AC
authors.lastname: Lepage
authors.forename: D
authors.initials: D
authors.lastname: Buzoni-Gatel
authors.forename: D T
authors.initials: DT
authors.lastname: Bout
mesh_terms: D000281:Administration, Intranasal; D019264:Adoptive Transfer; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002772:Cholera Toxin / Q000276:immunology; D018928:Immunity, Mucosal; D007114:Immunization; D007413:Intestinal Mucosa / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D009297:Nasal Mucosa / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C047168:SAG1 antigen, Toxoplasma; D002772:Cholera Toxin