Particle-mediated gene transfer of murine interleukin-12 cDNA suppresses the growth of Lewis lung carcinoma.
We evaluated the effectiveness of the Helios gene gun system, a recently developed, commercially available gene gun device. Following skin transfection with beta-galactosidase or interleukin-12 cDNA using the gene gun, beta-galactosidase expression was detected exclusively in the epidermal cell layer, and transgene expression of IL-12 cDNA was maximal 2 days post-transfection and remained detectable for at least 5 additional days. Furthermore, particle-mediated delivery of IL-12 cDNA into epidermal cells overlying an intradermal tumor resulted in a significant suppression of tumor growth of Lewis lung carcinoma. Appreciable levels of IFN-gamma production were readily detected at the skin transfection site, and were induced from splenocytes and lymph node cells in the IL-12 treated mice. These results show that in vivo delivery of IL-12 cDNA into skin by the Helios gene gun device can have a useful routine application for cancer therapy research.
Oshikawa
K
K
Department of Pulmonary Medicine, Jichi Medical School, Tochigi, Japan. kokyu2@jichi.ac.jp
Ishii
Y
Y
Hamamoto
T
T
Sugiyama
Y
Y
Kitamura
S
S
Kagawa
Y
Y
D000818:Animals; D019470:Biolistics / Q000379:methods*; D018827:Carcinoma, Lewis Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D002455:Cell Division / Q000235:genetics; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D018076:DNA, Complementary / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010316:Particle Size; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
D018076:DNA, Complementary; D018664:Interleukin-12; D007371:Interferon-gamma