Comparison of the glycosyl-phosphatidylinositol cleavage/attachment site between mammalian cells and parasitic protozoa.
It was previously hypothesised that the requirements for glycosyl-phosphatidylinositol (GPI) anchoring in mammalian cells and parasitic protozoa are similar but not identical. We have investigated this by converting the GPI cleavage/attachment site in porcine membrane dipeptidase to that found in the trypanosomal variant surface glycoprotein 117 and expressing the resulting mutants in COS-1 cells. Changing the entire (omega), (omega)+1 and (omega)+2 triplet in membrane dipeptidase from Ser-Ala-Ala to Asp-Ser-Ser resulted in efficient GPI anchoring of the mutant proteins, as assessed by cell-surface activity assays and susceptibility to release by phosphatidylinositol-specific phospholipase C. Immunoelectrophoretic blot analysis with antibodies recognising epitopes either side of the native (omega) residue in porcine membrane dipeptidase, and expression of a mutant in which potential alternative cleavage/attachment sites were disrupted, indicated that alternative GPI cleavage/attachment sites had not been used. These results indicate that the requirements for GPI anchoring between mammalian and protozoal cells are not as different as previously suggested, and that rules for predicting the probability of a sequence acting as a GPI cleavage/attachment site need to be applied with caution.
White
I J
IJ
School of Biochemistry, University of Leeds, Leeds LS2 9JT, UK.
Souabni
A
A
Hooper
N M
NM
D019881:Aminoacyltransferases / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology; D004150:Dipeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000502:physiology; D017261:Glycosylphosphatidylinositols / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008322:Mammals; D008565:Membrane Proteins / Q000235:genetics; D016297:Mutagenesis, Site-Directed / Q000502:physiology; D011817:Rabbits; D014162:Transfection; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000378:metabolism*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000032:analysis / Q000378:metabolism
D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D019881:Aminoacyltransferases; C023175:transamidases; D004150:Dipeptidases; C038937:dipeptidase