title: [Correlation of inactivation of CDKN2/p16 gene by methylation and lung cancers].
abstract: OBJECTIVE
  To study the relationship between methylation state of CDKN2/p16 gene and lung cancers.
  
  
  METHODS
  Genomic DNA was digested with methylation-sensitive enzymes and Southern blotting was used to analyses the methylation state of CDKN2/p16 gene in 89 cases of lung cancer.
  
  
  RESULTS
  Twenty-one out of 89 lung cancers(23.6%) shown methylation of CDKN2/p16 gene, and 17 of them occurred in 42 P16 negative cases with a rate of 40.5%(17/42).
  
  
  CONCLUSIONS
  Methylation of CDKN2/p16 gene 5' CpG island is probably an important mechanism of gene inactivation; it may take part in the onset and process of lung cancer.
authors.affiliation: Department of Molecular Biology, Nanjing 81 Hospital, Nanjing, Jiangsu, 210002 P.R. China. sucq@jlonline.com
authors.forename: C
authors.initials: C
authors.lastname: Su
authors.forename: X
authors.initials: X
authors.lastname: Shan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D019942:Genes, p16; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged