Differential regulation of NPY mRNA expression in embryonic sympathetic and chromaffin cultures by NGF.
RT-PCR analysis of NPY mRNA expression in chick embryonic sympathoadrenal cells in culture showed that NGF increases sympathetic but not adrenal NPY mRNA content. These results show that the previously reported differential effect at the protein level can also be detected at the mRNA level, suggesting a pre-translational point of regulation. The differential NGF effect in such closely related phenotypes is particularly relevant to studies of plasticity and differentiation.
Medina-Ortiz
W E
WE
Department of Biology, University of Puerto Rico, Box 23360, UPR Station, Rio Piedras, Puerto Rico.
García-Arrarás
J E
JE
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D019439:Chromaffin Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009478:Neuropeptide Y / Q000235:genetics* / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D009478:Neuropeptide Y; D012333:RNA, Messenger; D020932:Nerve Growth Factor; D019696:Peptidylprolyl Isomerase; D011188:Potassium