Expression and characterization of the 3a movement protein of cowpea chlorotic mottle bromovirus.
Cowpea chlorotic mottle bromovirus (CCMV) 3a protein is required for cell-to-cell movement of the virus in host plants. The 3a protein was produced in Escherichia coli using an expression vector. Gel retardation analysis and UV cross-linking experiments demonstrated that the CCMV 3a protein (CC3a) bound single-stranded (ss) RNA cooperatively without sequence specificity. Binding competition analysis showed that CC3a bound ss-nucleic acids more strongly than double-stranded nucleic acids.
Fujita
M
M
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Mise
K
K
Furusawa
I
I
D017795:Bromovirus / Q000235:genetics* / Q000502:physiology; D004247:DNA / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D053830:Plant Viral Movement Proteins; D010957:Plasmids / Q000235:genetics; D012330:RNA, Double-Stranded / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*
D004277:DNA, Single-Stranded; D053830:Plant Viral Movement Proteins; D012330:RNA, Double-Stranded; D018749:RNA, Plant; D011994:Recombinant Proteins; D014764:Viral Proteins; D012965:Sodium Chloride; D004247:DNA