Sympathoinhibition by adenosine A(1) receptors, but not P2 receptors, in the hamster mesenteric arterial bed.
The aim of the present study was to determine whether there are prejunctional inhibitory P2 purine receptors on sympathetic nerves in the hamster isolated perfused mesenteric arterial bed. Adenosine 5'-O-(3-thiotriphosphate (ATPgammaS; 10 microM), adenosine 5'-O-(2-thiodiphosphate) (ADPbetaS; 100 microM) and AMP (10 microM) had no significant effect on neurogenic contractions to electrical field stimulation. In contrast, P1 receptor agonists attenuated sympathetic vasoconstriction with a potency order of N(6)5'-(Nadenosine. The pEC(50) value for CPA was 7.5+/-0.1 (n=7). The concentration-inhibitory effect curve to CPA was shifted to the right by the adenosine A(1) receptor antagonist, 8-cyclopentyl-1, 3-dipropyl-xanthine (DPCPX; 10 nM; apparent pK(B) 9.6; n=6-7). In methoxamine raised-tone mesenteries CPA (0.001-10 microM) did not elicit vasorelaxation, and NECA and adenosine were only weak vasorelaxants. These results indicate that adenosine A(1) receptors, but not P2 receptors, inhibit prejunctionally sympathetic neurotransmission in the hamster mesenteric arterial bed.
Ralevic
V
V
School of Biomedical Sciences, Queen's Medical Centre, The University of Nottingham Medical School, Nottingham, UK. vera.ralevic@nottingham.ac.uk
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects / Q000294:innervation*; D008647:Mesocricetus; D008729:Methoxamine / Q000494:pharmacology; D018047:Receptors, Purinergic P1 / Q000502:physiology*; D018048:Receptors, Purinergic P2 / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects
D018047:Receptors, Purinergic P1; D018048:Receptors, Purinergic P2; C022571:adenosine 5'-O-(3-thiotriphosphate); D000255:Adenosine Triphosphate; D008729:Methoxamine