Oral tolerance elicited in mice by beta-lactoglobulin entrapped in biodegradable microspheres.
Oral administration of antigen is known to be appropriate for some vaccine purposes as well as oral tolerance induction. In the present study, oral administration of beta-lactoglobulin (BLG) loaded poly(D,L-lactide-co-glycolide) (D,L-PLG) microspheres induced tolerance was evaluated. A single feeding of 5 micrograms of encapsulated BLG tolerized BALB/c mice to subsequent BLG parenteral challenge, suppressing the specific humoral, intestinal and cellular responses. The tolerogenic efficient dose was then reduced 10,000 times, compared to oral administration of soluble BLG. This suggests that loading food proteins into D,L-PLG microspheres might be a potential tool for inducing oral tolerance with allergens.
Pecquet
S
S
Nestec SA, Nestlé Research Center Lausanne, Switzerland. sophie.pecquet@rdls.nestle.com
Leo
E
E
Fritsché
R
R
Pfeifer
A
A
Couvreur
P
P
Fattal
E
E
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis; D007441:Intubation, Gastrointestinal; D019344:Lactic Acid / Q000008:administration & dosage*; D007782:Lactoglobulins / Q000008:administration & dosage* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D011100:Polyglycolic Acid / Q000008:administration & dosage*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000008:administration & dosage*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D001672:Biocompatible Materials; D007782:Lactoglobulins; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D007073:Immunoglobulin E