title: Characteristics of human IgA and IgM genes used by plasma cells in the salivary gland resemble those used in duodenum but not those used in the spleen.
abstract: Immunologically, the parotid salivary gland is an effector site that secretes large quantities of polyspecific Abs into the saliva, mainly of the IgA isotype. It is considered to be part of the common mucosal immune system but the inductive site for the Ab-producing cells of the salivary gland has not yet been clearly identified. The origin and diversity of cells of B lineage can be investigated by analyzing their Ig heavy chain genes (IgH). We have obtained sequences of IgM and IgA VH4-34 genes from plasma cells in human salivary gland, duodenal lamina propria, and splenic red pulp. Related sequences were found in different areas sampled within each tissue studied, indicating that the plasma cells carrying these genes are widespread with limited diversity. Examples of related IgH genes that are isotype switched were also seen in the salivary gland. The genes from plasma cells of the salivary gland were highly mutated, as were duodenal plasma cell sequences. The level of mutation was significantly higher than that seen in splenic plasma cell sequences. Analysis of CDR3 regions showed that the sequences from salivary gland had significantly smaller CDR3 regions than sequences from spleen, due to differences in number and type of DH regions used. Sequences from duodenum also had smaller CDR3 regions. Therefore, plasma cells from human duodenum and salivary gland showed characteristics that differed from those of human splenic plasma cells.
authors.affiliation: Guy's, King's and St. Thomas' Medical School, Department of Histopathology, St. Thomas' Campus, Guy's, King's and St. Thomas' Medical School, The Rayne Institute, St. Thomas' Campus, and Guy's, King's and St. Thomas' School of Dentistry, De.
authors.forename: D K
authors.initials: DK
authors.lastname: Dunn-Walters
authors.forename: M
authors.initials: M
authors.lastname: Hackett
authors.forename: L
authors.initials: L
authors.lastname: Boursier
authors.forename: P J
authors.initials: PJ
authors.lastname: Ciclitira
authors.forename: P
authors.initials: P
authors.lastname: Morgan
authors.forename: S J
authors.initials: SJ
authors.lastname: Challacombe
authors.forename: J
authors.initials: J
authors.lastname: Spencer
mesh_terms: D001483:Base Sequence; D004386:Duodenum / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005803:Genes, Immunoglobulin; D006801:Humans; D007070:Immunoglobulin A / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D010306:Parotid Gland / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D010950:Plasma Cells / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000378:metabolism
chemical_list: D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region