Expression of intermediate filament proteins in benign lesions of the oral mucosa.
Immunohistochemistry with monospecific antibodies was used to study the expression patterns of cytokeratins (Cks) and vimentin in non-dysplastic lesions of the oral cavity, including lichen planus and fibromas. In hyperplastic lesions, Ck expression did not deviate significantly from the normal non-keratinizing squamous epithelium of the oral cavity. Hyperkeratotic lesions showed pronounced aberrations in their Ck profile. These lesions were characterized by extended expression of the keratinization marker Ck 10, the basal cell Ck 14 and the hyperproliferation-associated Ck 16 in the suprabasal compartment. The stratification markers Cks 4 and 13 showed a decreased expression. Coexpression of Cks and vimentin was found in lesions having accumulations of inflammatory cells in the subepithelial cell layer. These changes are felt to characterize benign mucosal lesions without dysplasia and might be helpful for distinguishing these lesions from potentially malignant ones.
van der Velden
L A
LA
Department of Otorhinolaryngology, Erasmus University Medical Centre Rotterdam, The Netherlands.
Manni
J J
JJ
Ramaekers
F C
FC
Kuijpers
W
W
D000911:Antibodies, Monoclonal; D002455:Cell Division; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005350:Fibroma / Q000737:chemistry / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis* / Q000235:genetics; D007633:Keratins / Q000032:analysis* / Q000235:genetics; D007972:Leukoplakia, Oral / Q000378:metabolism / Q000473:pathology; D017676:Lichen Planus, Oral / Q000378:metabolism / Q000473:pathology; D009059:Mouth Diseases / Q000378:metabolism* / Q000473:pathology; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology; D009062:Mouth Neoplasms / Q000737:chemistry / Q000473:pathology; D014746:Vimentin / Q000032:analysis* / Q000235:genetics
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D014746:Vimentin; D007633:Keratins