Early results from indirect drug susceptibility test for tubercle bacilli.
The indirect susceptibility test results on L-J medium for tubercle bacilli against streptomycin, isoniazid and rifampicin were read at the end of 2 wk and compared with the results at 4 wk. It was found that drug resistance could be correctly predicted in over 70 per cent of cultures including multi-drug resistant tuberculosis (MDR TB) strains at the end of 2 wk. The susceptibility to para-nitrobenzoic acid (PNB) read at 2 wk was able to distinguish non-tuberculous mycobacteria from Mycobacterium tuberculosis cultures. The early detection of resistance by this procedure requires only minimum inputs, and can benefit the majority of patients harbouring drug resistant tubercle bacilli.
Mathew
S
S
Tuberculosis Research Centre (ICMR), Chennai.
Narayanan
A
A
Segaran
R
R
Paramasivan
C N
CN
D000995:Antitubercular Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009579:Nitrobenzoates / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D018088:Tuberculosis, Multidrug-Resistant / Q000382:microbiology
D000995:Antitubercular Agents; D009579:Nitrobenzoates; C008629:4-nitrobenzoic acid; D007538:Isoniazid; D012293:Rifampin; D013307:Streptomycin