ppGpp accumulation in Pseudomonas aeruginosa and Pseudomonas fluorescens subjected to nutrient limitation and biocide exposure.
The effect of a commonly used biocide, 1,2-benzisothiazolin-3-one (BIT) on ppGpp accumulation in the pathogen, Pseudomonas aeruginosa PAO1, and an environmental isolate, Ps. fluorescens, was examined. It is concluded that BIT is able to induce a stringent response in Ps. aeruginosa and Ps. fluorescens, determined by the rapid accumulation of ppGpp following addition of BIT to exponentially-growing cells. Western blot analysis of whole-cell extracts with anti-RelA antibody demonstrated that both species contain a RelA homologue. This is the first report of a RelA-like protein in pseudomonads.
Greenway
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DL
Department of Biological Sciences, University of Central Lancashire, Preston, UK.
England
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RR
D015153:Blotting, Western; D003470:Culture Media; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009584:Nitrogen / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D015203:Reproducibility of Results; D013844:Thiazoles / Q000494:pharmacology*
D003470:Culture Media; D013844:Thiazoles; D006159:Guanosine Tetraphosphate; C015699:1,2-benzisothiazoline-3-one; D009584:Nitrogen