A simplified method of generating transgenic Xenopus.
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.
Sparrow
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DB
Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK. dsparro@nimr.mrc.ac.uk
Latinkic
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B
Mohun
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TJ
D000818:Animals; D030801:Animals, Genetically Modified; D005306:Fertilization; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D010063:Ovum; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa; D014981:Xenopus / Q000235:genetics*; D014982:Xenopus laevis
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins