DBA/2J (Mls-1a) B-cell differentiation in BALB.xid recipients.
Studies of superantigens (SAg) have focused primarily on their impact on CD4+ T cells, largely bypassing the impact of the sequelae of this interaction upon the antigen-presenting cell (APC). Sequelae of SAg-induced CD4+ T-cell activation include the 'bathing' of the SAg-presenting cell with cytokines that promote the differentiation of the APC. In this report, the SAg-induced differentiation of Mls+ DBA/2J B cells was studied in vivo by their transplantation into B-cell-defective BALB.xid recipients. Rapid, high-level serum immunoglobulin M (IgM) production was noted shortly after transfer, disappearing by 3 weeks. Donor B cells, as evidenced after their chemical and genetic impairment and by the use of an IgM allotype-disparate donor-recipient combination, contributed to this transient IgM production. These results clarify a discrepancy in the literature regarding donor B-cell contribution to IgM production and illustrate a model system to utilize SAg to study B-lymphocyte diversity.
Tocce
K
K
Department of Biology, Rider University, Lawrenceville, NJ 08648-3099, USA.
Suppiah
K
K
Rago
C
C
Liberchuk
R
R
Duffy
K
K
Riggs
J
J
D019264:Adoptive Transfer; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D007075:Immunoglobulin M / Q000097:blood*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D016513:Mice, SCID; D016685:Mitomycin / Q000494:pharmacology; D018089:Superantigens / Q000494:pharmacology
D007075:Immunoglobulin M; D018089:Superantigens; D016685:Mitomycin