title: Application of 16S rRNA gene sequencing to identify Bordetella hinzii as the causative agent of fatal septicemia.
abstract: We report on the first case of fatal septicemia caused by Bordetella hinzii. The causative organism exhibited a biochemical profile identical to that of Bordetella avium with three commercial identification systems (API 20E, API 20 NE, and Vitek GNI+ card). However, its cellular fatty acid profile was not typical for either B. avium or previously reported strains of B. hinzii. Presumptive identification of the patient's isolate was accomplished by traditional biochemical testing, and definitive identification was achieved by 16S rRNA gene sequence analysis. Phenotypic features useful in distinguishing B. hinzii from B. avium were production of alkali from malonate and resistance to several antimicrobial agents.
authors.affiliation: Departments of Laboratory Medicine, Division of Bioinformatics, University of Washington, Seattle, Washington 98195, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Kattar
authors.forename: J F
authors.initials: JF
authors.lastname: Chavez
authors.forename: A P
authors.initials: AP
authors.lastname: Limaye
authors.forename: S L
authors.initials: SL
authors.lastname: Rassoulian-Barrett
authors.forename: S L
authors.initials: SL
authors.lastname: Yarfitz
authors.forename: L C
authors.initials: LC
authors.lastname: Carlson
authors.forename: Y
authors.initials: Y
authors.lastname: Houze
authors.forename: S
authors.initials: S
authors.lastname: Swanzy
authors.forename: B L
authors.initials: BL
authors.lastname: Wood
authors.forename: B T
authors.initials: BT
authors.lastname: Cookson
mesh_terms: D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001884:Bordetella / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D001885:Bordetella Infections / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D017809:Fatal Outcome; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S