title: Isolation of Angiostrongylus costaricensis first-stage larvae from rodent feces on a Percoll gradient.
abstract: Several density gradients were tested for the isolation of parasitic nematode, Angiostrongylus costaricensis, first-stage larvae from rodent feces. With a 45/72% Percoll gradient, 83-99% (89.56+/-6.57%) of the larvae were recovered in a clean preparation.
authors.affiliation: Laboratórios de Biologia Parasitária e Parasitologia Molecular, Faculdade de Biociências e Instituto de Pesquisas Biomédicas, PUCRS, Porto Alegre, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Graeff-Teixeira
authors.forename: S
authors.initials: S
authors.lastname: Geiger
authors.forename: B
authors.initials: B
authors.lastname: Walderich
authors.forename: W
authors.initials: W
authors.lastname: Hoffmann
authors.forename: E
authors.initials: E
authors.lastname: Abrahams
authors.forename: H
authors.initials: H
authors.lastname: Schulz-Key
mesh_terms: D000801:Angiostrongylus / Q000302:isolation & purification*; D000818:Animals; D002499:Centrifugation, Density Gradient; D003102:Colloids; D005243:Feces / Q000469:parasitology*; D007814:Larva; D051379:Mice; D008809:Mice, Inbred C3H; D011205:Povidone; D012376:Rodent Diseases / Q000469:parasitology*; D012822:Silicon Dioxide; D017206:Strongylida Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D003102:Colloids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone