Potato mop-top virus RNA can move long distance in the absence of coat protein: evidence from resistant, transgenic plants.
Transgenic expression of a translatable version of the Potato mop-top virus (PMTV) coat protein (CP) gene (encoded by RNA 3) in Nicotiana benthamiana prevented production of symptoms and infective virus particles. RNAs 1 and 2 accumulated in inoculated and systemic leaves but, apart from small amounts of CP transgene RNA transcript, no genomic-length RNA 3 was found. Crude leaf extracts from inoculated plants were not infective. However, when RNA extracts from such transgenic plants were inoculated to nontransgenic N. benthamiana and N. clevelandii, RNA 1 and RNA 2 replicated in systemic leaves of both species in the absence of RNA 3 and virus particles, but symptoms did not develop. We suggest that the triple-gene block proteins of PMTV (encoded by RNA 2) represent a class of long-distance RNA movement factors.
McGeachy
K D
KD
Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Barker
H
H
D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000821:virology; D010947:Plants, Toxic; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D011198:Solanum tuberosum / Q000235:genetics / Q000821:virology*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000821:virology
D012367:RNA, Viral