title: Vulvar lichen sclerosus: an immunologic study.
abstract: OBJECTIVE
  To investigate the seroimmunologic (CD3, CD4, CD8 lymphocytes, C3c and C4 complement fractions, and several autoantibodies) and immunohistochemical (T lymphocyte subpopulations, B lymphocytes, natural killer cells, macrophages, immunoglobulin [Ig] G, Ig M, and C3c complement fraction) characteristics of vulvar lichen sclerosus.
  
  
  METHODS
  Serum samples from 68 women with histologically proven lichen sclerosus were compared with those from 53 healthy controls, and tissue samples from 14 of 68 women chosen at random were compared with those from 14 of 53 healthy controls. A scoring system was constructed to compare the number of cells in the tissue.
  
  
  RESULTS
  Patients had significantly lower counts of circulating lymphocytes CD3 and CD4 than controls (P < .05) and a higher number of autoantibodies (P < .01). Analysis of the tissue samples confirmed a lower number of CD2 cells (two-tailed P = .002 in epidermis, .005 in dermis), CD3 cells (two-tailed P = .001 in epidermis and in dermis), CD4 cells (two-tailed P = .002 in epidermis, .011 in dermis), and CD8 cells (two-tailed P = .002 in epidermis, .051 in dermis) in subjects than in controls. Numbers of monocyte-macrophage cells were similar in the epidermis but different in the dermis (two-tailed P = .003). No natural killer CD56 cells or B lymphocytes (CD19-CD21) were detected in the affected areas. Deposits of IgG, IgM, and C3 were no greater in biopsy specimens of patients than in those of controls.
  
  
  CONCLUSIONS
  Vulvar lichen sclerosus is not caused by a T cell-mediated response, and a viral origin is unlikely. The absence of CD19 and CD21 cells excludes local production of autoantibodies. Our data do not confirm an autoimmune pathogenesis for vulvar lichen sclerosus but help explain why systemic cortisone is of no benefit and justify the use of petroleum jelly to relieve pruritus.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Trieste, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Scrimin
authors.forename: S
authors.initials: S
authors.lastname: Rustja
authors.forename: O
authors.initials: O
authors.lastname: Radillo
authors.forename: C
authors.initials: C
authors.lastname: Volpe
authors.forename: R
authors.initials: R
authors.lastname: Abrami
authors.forename: S
authors.initials: S
authors.lastname: Guaschino
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015551:Autoimmunity; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007150:Immunohistochemistry; D007694:Killer Cells, Natural / Q000276:immunology; D018459:Lichen Sclerosus et Atrophicus / Q000276:immunology*; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014845:Vulvar Diseases / Q000276:immunology*