title: Expression of calcium-sensing receptor gene by avian parathyroid gland in vivo: relationship to plasma calcium.
abstract: Calcium-sensing receptor (CaR) gene expression and parathyroid hormone (PTH) content were evaluated in situ in chicken parathyroid glands (PG) in relation to changes in plasma calcium. The CaR gene is expressed by the parathyroid chief cells, the same cells that store and secrete PTH. An increase in plasma calcium, achieved by repletion of vitamin D-deficient chicks with a normal diet, by PTH injection, or during eggshell formation, increased the expression of the CaR gene. Low plasma calcium concentration in vitamin D-deficient chicks or in layers, before or after eggshell formation, was associated with decrease in CaR gene expression in the PG. The level of CaR gene expression was inversely correlated with the PTH content of the PG. The results of this study demonstrate for the first time that, in contrast to mammals, the CaR gene expression in the PG of the chicken is inversely associated with changes in plasma calcium.
authors.affiliation: Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Yarden
authors.forename: I
authors.initials: I
authors.lastname: Lavelin
authors.forename: O
authors.initials: O
authors.lastname: Genina
authors.forename: S
authors.initials: S
authors.lastname: Hurwitz
authors.forename: R
authors.initials: R
authors.lastname: Diaz
authors.forename: E M
authors.initials: EM
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Pines
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000097:blood*; D002645:Chickens / Q000378:metabolism*; D004032:Diet; D004528:Egg Shell; D005786:Gene Expression Regulation / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D010280:Parathyroid Glands / Q000378:metabolism*; D044169:Receptors, Calcium-Sensing; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D014808:Vitamin D Deficiency / Q000378:metabolism
chemical_list: D044169:Receptors, Calcium-Sensing; D011956:Receptors, Cell Surface; D002118:Calcium