title: Effect of various concentrations of acyclovir on cell survival and micronuclei induction on cultured HeLa cells.
abstract: The HeLa cells were treated with 0, 0.01, 0.1, 1, 10 and 100 microM acyclovir (ACV) for 8 h duration and the growth kinetics, cell survival and micronuclei induction were determined. Treatment of HeLa cells with various concentrations of ACV resulted in a concentration-dependent decline in growth kinetics, cell proliferation indices and cell survival. ACV, 100 microM, completely inhibited cell division, where no appreciable changes in cell number were observed from 1 to 5 days post-treatment. This is reflected in cell survival, where the surviving fraction of cells was reduced to 1/2 at 100 microM ACV. Conversely, the frequency of micronuclei showed a concentration-dependent elevation at 20, 30 and 40 h post-treatment. ACV not only induced one micronuclei-bearing binucleate cell but also binucleate cells bearing two and multiple micronuclei in a concentration-dependent manner. The micronuclei frequency increased with time up to 30 h post-treatment and declined thereafter. The relationship between micronuclei induction and cell survival was determined by plotting the former on Y- and the latter on X-axes, respectively. The surviving fraction of cells declined with the elevation in micronuclei frequency and a best fit was observed for linear quadratic formalism.
authors.affiliation: Department of Radiobiology, Kasturba Medical College, Manipal, India. info@kmc.ernet.in
authors.forename: G C
authors.initials: GC
authors.lastname: Jagetia
authors.forename: R
authors.initials: R
authors.lastname: Aruna
mesh_terms: D000212:Acyclovir / Q000008:administration & dosage / Q000494:pharmacology*; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics*; D002470:Cell Survival / Q000187:drug effects* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D015162:Micronucleus Tests; D014410:Tumor Stem Cell Assay / Q000379:methods
chemical_list: D000998:Antiviral Agents; D000212:Acyclovir