title: [Cardiolipin antibodies dependents on beta2-glycoprotein-1 in antiphospholipid syndrome].
abstract: OBJECTIVE
  To study beta2-GP-I-dependent binding of phospholipid antibodies (PAb) to phospholipids and this process participation in pathogenesis of antiphospholipid syndrome (APS).
  
  
  METHODS
  IgG-fractions and sera from 20 patients with APS. Cofactor activity of beta2-GP-I isolated from serum of healthy donors was examined with modified immunoassay.
  
  
  RESULTS
  Contrary to donor IgG, binding of IgG fractions isolated from sera of APS patients with cardiolipin grows dose-dependently in the presence of beta2-GP-I. Cofactor activity of beta2-GP-I is confirmed in the study of sera of APS patients. Sera containing beta2-GP-I-dependent antibodies to cardiolipin (aCL), unlike aCL-negative sera, react with solid-phase immobilized beta2-GP-I.
  
  
  CONCLUSIONS
  It is confirmed that beta2-GP-I participates in interaction of PAb with cardiolipin. Pathogenetic implication of beta2-GP-I-dependent PAb for onset of APS is discussed.
authors.forename: T V
authors.initials: TV
authors.lastname: Kuznetsova
authors.forename: V A
authors.initials: VA
authors.lastname: Tishchenko
authors.forename: A G
authors.initials: AG
authors.lastname: Kobylianskiĭ
authors.forename: M E
authors.initials: ME
authors.lastname: Pal'keeva
authors.forename: A A
authors.initials: AA
authors.lastname: Novikov
authors.forename: T M
authors.initials: TM
authors.lastname: Reshetniak
authors.forename: N G
authors.initials: NG
authors.lastname: Kliukvina
authors.forename: E L
authors.initials: EL
authors.lastname: Nasonov
mesh_terms: D000328:Adult; D017153:Antibodies, Anticardiolipin / Q000276:immunology*; D016736:Antiphospholipid Syndrome / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D001666:Binding Sites, Antibody; D015415:Biomarkers / Q000097:blood; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007118:Immunoassay; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood* / Q000276:immunology; D011379:Prognosis; D053482:beta 2-Glycoprotein I
chemical_list: D017153:Antibodies, Anticardiolipin; D015415:Biomarkers; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053482:beta 2-Glycoprotein I