Genetic characterization of the silkworm Bombyx mori by simple sequence repeat (SSR)-anchored PCR.
Thirteen diverse strains of the silkworm Bombyx mori were analysed using the simple sequence repeat anchored polymerase chain reaction (SSR-anchored PCR) or Inter-SSR-PCR (ISSR-PCR). A set of four 5'-anchored and two 3'-anchored repeat primers amplified a total of 239 bands out of which 184 (77%) were polymorphic. The 5'-anchored primers revealed more distinct polymorphic markers than the 3'-anchored primers and the ISSR-PCR method showed greater variability than RAPDs. The strain-specific pattern was shown to be inherited and segregated in a Mendelian fashion. A dendrogram constructed using the UPGMA method revealed two distinct groups, one comprising nondiapausing and one comprising diapausing strains. These results suggest that the ISSR-PCR method is potentially useful for genetic fingerprinting of silkworm genotypes and as a mapping tool in the silkworm.
Reddy
K D
KD
Seribiotech Research Laboratory, Central Silk Board, No. 8, West of Chord Road, Bangalore-560 086, India.
Nagaraju
J
J
Abraham
E G
EG
D000818:Animals; D012831:Bombyx / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction