Discovery of a putative heme-binding protein family (SOUL/HBP) by two-tissue suppression subtractive hybridization and database searches.
In the domestic chicken, Gallus gallus, the retina and pineal gland contain circadian clocks that are directly entrained by environmental light-dark cycles. To identify novel genes that are expressed in the retina and pineal gland, we performed two-tissue suppression subtractive hybridization (SSH). Two-tissue SSH is designed to identify genes expressed in common between two RNA samples while at the same time subtracting out abundant transcripts. Using this method, we identified a novel chicken gene, named ckSoul, that is strongly expressed in the retina and pineal gland. The protein product of ckSoul is similar to a novel heme-binding protein (p22 HBP) and to an uncharacterized mammalian gene in the expressed sequence tag (EST) database. The mouse transcript of this new gene is expressed in the retina and may represent the mammalian ortholog of ckSoul. Molecular analysis of the mammalian and chicken proteins suggests SOUL and HBP are members of a new family of heme-binding proteins.
Zylka
M J
MJ
Laboratory of Developmental Chronobiology, Pediatric Service, Massachusetts General Hospital, and Harvard Medical School, Boston, MA 02114, USA.
Reppert
S M
SM
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D016208:Databases, Factual; D005260:Female; D015870:Gene Expression; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010870:Pineal Gland / Q000378:metabolism; D011257:Pregnancy Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D002352:Carrier Proteins; D018076:DNA, Complementary; C516258:HEBP2 protein, human; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D011257:Pregnancy Proteins; D012313:RNA