title: Characterization of TEM-56, a novel beta-lactamase produced by a Klebsiella pneumoniae clinical isolate.
abstract: TEM-56 produced by a Klebsiella pneumoniae clinical isolate is a novel beta-lactamase of isoelectric point 6.4 that confers a moderate resistance level to expanded-spectrum cephalosporins. The amino acid sequence deduced from the corresponding bla gene showed two amino acid replacements with respect to the TEM-2 sequence: Glu-104 to Lys and His-153 to Arg. This enzyme showed catalytic properties close to those of TEM-18. Thus, TEM-56 appears as a new TEM mutant, an intermediary between TEM-18 and the extended-spectrum beta-lactamase TEM-21.
authors.affiliation: Laboratoire de Bact¿eriologie, H¿opital Universitaire du Bocage, B.P. 1542, 21034 Dijon Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Neuwirth
authors.forename: R
authors.initials: R
authors.lastname: Labia
authors.forename: E
authors.initials: E
authors.lastname: Siebor
authors.forename: A
authors.initials: A
authors.lastname: Pechinot
authors.forename: S
authors.initials: S
authors.lastname: Madec
authors.forename: E B
authors.initials: EB
authors.lastname: Chaibi
authors.forename: A
authors.initials: A
authors.lastname: Kazmierczak
mesh_terms: D019943:Amino Acid Substitution; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: C405500:TEM-56 beta-lactamase; D001618:beta-Lactamases