title: Granulocyte macrophage-colony stimulating factor stimulates secretion of chemoattractive cytokines by peritoneal macrophages of CAPD patients.
abstract: The recruitment of immunocompetent cells to the site of inflammation represents an essential part of the host defense during continuous ambulatory peritoneal dialysis (CAPD)-related peritonitis. Recently, it was shown that intraperitoneal application of granulocyte macrophage-colony stimulating factor (GM-CSF) leads to a marked transient recruitment of macrophages, paralleled by an increase in monocyte chemoattractant protein (MCP)-1. We, therefore, tested the in vitro effect of GM-CSF on the release of the chemotaxins interleukin (IL)-8 and MCP-1 by human peritoneal macrophages. Cells were stimulated with recombinant GM-CSF for 4, 12, and 20 hours in concentrations ranging from 0.1 to 100 pg/mL. Cells stimulated with lipopolysaccharide (LPS) or unstimulated cells served as control. Recombinant GM-CSF at concentrations found during CAPD peritonitis in vivo significantly increased the release of IL-8 and MCP-1 in a time- and dose-dependent manner. The maximum effect of IL-8 was observed directly after cell isolation, and decreased after a culture period of 10 days. Thus, our results indicate that peritoneal macrophages are the potential source of chemokines released upon GM-CSF stimulation.
authors.affiliation: Abteilung Innere Medizin IV, Nephrologie, Universitaetsklinik Freiburg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Schäfer
authors.forename: S
authors.initials: S
authors.lastname: Stöteknuel
authors.forename: P
authors.initials: P
authors.lastname: Schollmeyer
authors.forename: G J
authors.initials: GJ
authors.lastname: Dobos
mesh_terms: D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D010531:Peritoneal Dialysis, Continuous Ambulatory
chemical_list: D018932:Chemokine CCL2; D016209:Interleukin-8; D016178:Granulocyte-Macrophage Colony-Stimulating Factor