Expression of canine interferon-beta by a recombinant vaccinia virus.
A recombinant vaccinia virus expressing canine interferon (IFN)-beta was constructed (vv/cIFN-beta). In rabbit kidney (RK13) and canine A72 cells infected with vv/cIFN-beta, the recombinant canine IFN-beta was detected in both cell extracts and supernatants, and the IFN activities of the culture supernatants were also detected. Inhibition of N-linked glycosylation by tunicamycin treatment indicated that the recombinant canine IFN-beta was modified by N-linked glycosylation in a different way between RK13 and A72 cells, and that N-linked glycosylation is essential for its secretion. The growth of vv/cIFN-beta at a low multiplicity of infection was inhibited by antiviral activity of canine IFN-beta, indicating that this recombinant virus could be used as a suicide viral vector.
Nishikawa
Y
Y
The Research Center for Protozoan Molecular Immunology, Obihiro University, Inadacho, Obihiro, Hokkaido, Japan.
Iwata
A
A
Xuan
X
X
Nagasawa
H
H
Fujisaki
K
K
Otsuka
H
H
Mikami
T
T
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004285:Dogs; D015870:Gene Expression; D005822:Genetic Vectors; D006031:Glycosylation; D007370:Interferon Type I / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D007700:Kinetics; D011817:Rabbits; D011994:Recombinant Proteins; D014616:Vaccinia virus / Q000235:genetics* / Q000254:growth & development
D017931:DNA Primers; D007370:Interferon Type I; D011994:Recombinant Proteins