Electromagnetic fields enhance the stress response at elevated temperatures in the nematode Caenorhabditis elegans.
We have studied the effect of extremely low frequency electromagnetic fields (ELF-EMF) in the presence of a second stressor (mild heat shock) on the expression of a lacZ reporter gene under the control of hsp16 or hsp70 promoters in two transgenic strains of C. elegans. The expression of the reporter gene was studied by scoring animals with induced beta-galactosidase activity after staining in toto or by biochemical quantitation of the enzyme activity, respectively. In our experimental setup we were able to expose the animals to 50 Hz magnetic flux density of 0-150 microT and at the same time control temperature with high precision (+/-0.1 degrees C). Experimental conditions were defined for which EMF strongly enhances the expression of the reporter gene.
Junkersdorf
B
B
Institut für Zoologie, Technische Universität Dresden, Dresden, Germany.
Bauer
H
H
Gutzeit
H O
HO
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000502:physiology* / Q000528:radiation effects*; D029742:Caenorhabditis elegans Proteins; D004574:Electromagnetic Fields; D005786:Gene Expression Regulation / Q000528:radiation effects; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D011401:Promoter Regions, Genetic / Q000528:radiation effects; D001616:beta-Galactosidase / Q000235:genetics
D029742:Caenorhabditis elegans Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D001616:beta-Galactosidase