Endomysial antibody production is not related to histological damage after in vitro gluten challenge.
OBJECTIVE
To determine whether in vitro induction of endomysial antibodies is an in vitro marker of coeliac toxicity.


METHODS
To determine whether in vitro endomysial antibodies induced by gliadin incubation correlate with histological damage induced by in vitro gliadin challenge.


METHODS
Small-bowel organ cultures from seven patients with treated coeliac disease were incubated in an organ culture system with gliadin; histological damage was morphometrically evaluated and endomysial antibodies were measured in the organ culture supernatant by indirect immunofluorescence.


RESULTS
Although incubation with gliadin caused histological damage, there was no detectable production of endomysial antibody.


CONCLUSIONS
In vitro, endomysial antibody induction cannot be used as a marker of coeliac toxicity. Endomysial antibodies are not necessary for generating the histological lesion of coeliac disease.
Biagi
F
F
Gastroenterology Unit, GKT, St Thomas' Hospital, London, UK.
Parnell
N D
ND
Ellis
H J
HJ
Ciclitira
P J
PJ
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis* / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002446:Celiac Disease / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology*; D004386:Duodenum / Q000276:immunology / Q000473:pathology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005903:Gliadin / Q000276:immunology / Q000633:toxicity*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000276:immunology; D009924:Organ Culture Techniques; D011237:Predictive Value of Tests
D001323:Autoantibodies; D015415:Biomarkers; D005903:Gliadin