Lineage-restricted expression of bone morphogenetic protein genes in human hematopoietic cell lines.
To explore the possibility that bone morphogenetic proteins (BMPs) are autocrine/paracrine regulators of hematopoietic differentiation and function, we screened a panel of human cell lines encompassing the hematopoietic lineages for expression of members of this family of genes. Expression of BMP-2, BMP-4, BMP-6, BMP-7, Growth and Differentiation Factor-1 (GDF-1), Placental Bone Morphogenetic Protein (PLAB), and Transforming Growth Factor-beta3 (TGF-beta3) was detected in one or more cell lines. BMP-2, BMP-4, BMP-7, and TGF-beta3 expression was also found in normal hematopoietic tissue. Expression of BMP-5 and BMP-8 was not seen. Lineage-restricted patterns of expression were found for BMP-4 (T-lymphoid), BMP-7 (lymphoid), PLAB (macrophage/monocyte), and GDF-1 (myeloid). Expression of BMP-2, GDF-1, and PLAB could be modulated by treatment with differentiating agents. Marked variations in the levels of BMP-4, BMP-7, and PLAB expression were encountered, indicating that disorders in BMP signaling pathways may play a role in the development of hematopoietic neoplasia.
Detmer
K
K
Division of Basic Medical Sciences, Mercer University School of Medicine, Macon, GA 31207, USA. detmer.k@gain.mercer.edu
Steele
T A
TA
Shoop
M A
MA
Dannawi
H
H
D001854:Bone Marrow Cells / Q000737:chemistry / Q000502:physiology; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D055417:Bone Morphogenetic Protein 5; D055418:Bone Morphogenetic Protein 6; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D016207:Cytokines; D015870:Gene Expression; D005786:Gene Expression Regulation; D055452:Growth Differentiation Factor 1; D055436:Growth Differentiation Factor 15; D006133:Growth Substances / Q000235:genetics / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008264:Macrophages / Q000737:chemistry / Q000502:physiology; D009000:Monocytes / Q000737:chemistry / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology; D011257:Pregnancy Proteins / Q000235:genetics / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000502:physiology
C525718:BMP2 protein, human; C525809:BMP4 protein, human; C525814:BMP5 protein, human; C525816:BMP6 protein, human; C525819:BMP7 protein, human; C485773:BMP8A protein, human; C405632:BMP8B protein, human; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D055417:Bone Morphogenetic Protein 5; D055418:Bone Morphogenetic Protein 6; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D016207:Cytokines; C525977:GDF1 protein, human; C526140:GDF15 protein, human; D055452:Growth Differentiation Factor 1; D055436:Growth Differentiation Factor 15; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011257:Pregnancy Proteins; D016212:Transforming Growth Factor beta