Role of the PDR gene network in yeast susceptibility to the antifungal antibiotic mucidin.
Yeast strains disrupted in the PDR1, PDR3, or PDR5 gene, but not in SNQ2, exhibited higher sensitivity to mucidin (strobilurin A) than did the isogenic wild-type strains. Different gain-of-function mutations in the PDR1 and PDR3 genes rendered yeast mutants resistant to this antibiotic. Mucidin induced PDR5 expression, but the changes in the expression of SNQ2 were only barely detectable. The results indicate that PDR5 provides the link between transcriptional regulation by PDR1 and PDR3 and mucidin resistance of yeast.
Michalkova-Papajova
D
D
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
Obernauerova
M
M
Subik
J
J
D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000502:physiology; D000935:Antifungal Agents / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D008689:Methacrylates; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D000073739:Strobilurins; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D000935:Antifungal Agents; D004268:DNA-Binding Proteins; D005231:Fatty Acids, Unsaturated; D008565:Membrane Proteins; D008689:Methacrylates; C085480:PDR1 protein, S cerevisiae; C089261:PDR3 protein, S cerevisiae; C092438:PDR5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000073739:Strobilurins; D015534:Trans-Activators; D014157:Transcription Factors; C008556:mucidin