title: Characterization of a human pancreatic secretory trypsin inhibitor mutant binding to Legionella pneumophila as determined by a quartz crystal microbalance.
abstract: We describe the isolation from a large phagemid library of a human pancreatic secretory trypsin inhibitor (hPSTI) mutant that binds to Legionella pneumophila. To gain further insight into the binding kinetics of the isolated hPSTI mutant, an immunosensing system based on a quartz crystal microbalance (QCM) was used. In contrast to ELISA procedures, k(on) and k(off) rates could be derived from the QCM sensograms. Thus, it is possible to characterize specific intermolecular interactions between proteins and phages isolated from large phage display libraries by QCM.
authors.affiliation: Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz Josef Strauss Allee 11, 93053, Regensburg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Decker
authors.forename: K
authors.initials: K
authors.lastname: Weinberger
authors.forename: E
authors.initials: E
authors.lastname: Prohaska
authors.forename: S
authors.initials: S
authors.lastname: Hauck
authors.forename: C
authors.initials: C
authors.lastname: Kösslinger
authors.forename: H
authors.initials: H
authors.lastname: Wolf
authors.forename: A
authors.initials: A
authors.lastname: Hengerer
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D016952:Legionella pneumophila / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D010179:Pancreas / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D011485:Protein Binding; D011487:Protein Conformation; D011791:Quartz; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D010446:Peptide Fragments; D019151:Peptide Library; D014361:Trypsin Inhibitors; D011791:Quartz