AMY-1 is a trigger for the erythrocyte differentiation of K562 cells.
We have reported that a novel c-Myc binding protein, AMY-1, stimulated the transcription activity of c-Myc and was translocated from cytoplasm to nuclei in a c-Myc-dependent manner. Here, the role of AMY-1 in cell differentiation was examined. AMY-1 expression was up-regulated after differentiation induction of human K562 cells to erythrocyte cells by AraC, while c-Myc expression was rapidly down-regulated. K562 cell lines expressing exogenous AMY-1 were established, and these cells expressed a high level of epsilon-globin mRNA, a marker gene necessary for erythrocyte cell differentiation, without differentiation induction. The addition of AraC rapidly initiated differentiation in these cell lines, which continued to differentiate to erythrocyte cells possessing a high level of hemoglobin even after the decrease in AMY-1 expression. These results suggest that AMY-1 is a trigger for K562 cells to differentiate to erythrocyte cells and that AMY-1 may have a function independent of or different from c-Myc.
Furusawa
M
M
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo 060-0812, Japan.
Onishi
T
T
Taira
T
T
Iguchi-Ariga
S M
SM
Ariga
H
H
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D002454:Cell Differentiation / Q000502:physiology*; D003561:Cytarabine / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000502:physiology*; D005914:Globins / Q000378:metabolism; D006801:Humans; D020014:K562 Cells / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors / Q000502:physiology*
D000964:Antimetabolites, Antineoplastic; D004268:DNA-Binding Proteins; C116115:MYCBP protein, human; D012333:RNA, Messenger; D014157:Transcription Factors; D003561:Cytarabine; D005914:Globins