Crossreactive neutralizing antibodies induced by immunization with caprine arthritis-encephalitis virus surface glycoprotein.
Four Saanen goats were immunized with affinity purified gp135 surface glycoprotein (SU) of caprine arthritis-encephalitis virus isolate 79-63 (CAEV-63) and evaluated for homologous and crossreactive serum neutralizing antibodies. CAEV-63 neutralizing antibodies were detected in all goats after seven immunizations with SU in Quil A adjuvant. Sera from three goats neutralized an independent CAEV isolate (CAEV-Co). However, serum from one goat did not detectably neutralize heterologous CAEV-Co and inhibited CAEV-Co neutralization by another serum.
Kemp
R K
RK
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
Knowles
D P
DP
Perry
L L
LL
McGuire
T C
TC
Besser
T E
TE
Cheevers
W P
WP
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism*; D000918:Antibody Specificity; D016088:Arthritis-Encephalitis Virus, Caprine / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D058967:Contactin 1; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006041:Goats; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D014765:Viral Vaccines / Q000276:immunology
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D058967:Contactin 1; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D014765:Viral Vaccines