title: Mesothelial regeneration is not dependent on subserosal cells.
abstract: It has been proposed that after mesothelial injury, resident cells within the subserosal connective tissue proliferate, differentiate, and migrate to the serosal surface. The aim of this study was to examine the temporal and spatial changes of proliferating cells in a murine model of testicular mesothelial healing and assess the potential of submesothelial cells to reconstitute the damaged mesothelium. Histology and autoradiography were employed to determine the number of cells within the submesothelial connective tissue, as well as the proportion of cells undergoing DNA synthesis on and beneath the injured serosa. Mesothelial cells surrounding the wound demonstrated maximal DNA synthesis 48 h after injury (27. 82+/-5.64% SEM, compared with 0.17+/-0.16% (3)H-TdR labelled cells for resting mesothelium), whereas a significant increase in proliferating submesothelial cells was not seen until day 4 post-injury (7.79+/-3.31% compared with 0.85+/-0.64% (3)H-TdR labelled cells at day 2). Furthermore, this small number of dividing submesothelial cells must include cells other than the proposed mesothelial precursors, indicating a very low proportion of precursor cells in the submesothelial cell population. As large numbers of mesothelial cells were seen at the wound centre by 3-4 days after injury, it is unlikely that submesothelial cells contributed significantly to the repopulation of the injured mesothelium. It is hypothesized that regenerating mesothelium is more likely to originate from the surrounding uninjured mesothelial cell population.
authors.affiliation: Department of Pathology, University of Western Australia, Western Australia, Australia.
authors.forename: S E
authors.initials: SE
authors.lastname: Mutsaers
authors.forename: D
authors.initials: D
authors.lastname: Whitaker
authors.forename: J M
authors.initials: JM
authors.lastname: Papadimitriou
mesh_terms: D000818:Animals; D001345:Autoradiography; D002452:Cell Count; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D004848:Epithelium / Q000473:pathology / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012038:Regeneration / Q000502:physiology*; D013737:Testis / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D004247:DNA