Protective effects of Orostachys japonicus A. Berger (Crassulaceae) on H2O2-induced apoptosis in GT1-1 mouse hypothalamic neuronal cell line.
Oxidative stress is a major cause of neurodegenerative diseases, so the protection of neuronal cells from reactive oxygen species can be beneficial for the prevention and treatment of these diseases. Methanol extract of Orostachys japonicus A. Berger (Crassulaceae), a traditional oriental medical herb, was shown to have a protective effect on H2O2-induced apoptosis in GT1-1 mouse hypothalamic neuronal cell line which was detected by flow cytometry after propidium iodide staining. Among fractions of O. japonicus, chloroform fraction had the highest protective effect, and water fraction had no protective effect suggesting that the active ingredients might be hydrophobic compounds.
Yoon
Y
Y
Aging and Regimen Research Team, Korea Institute of Oriental Medicine, Seoul, South Korea. ysyoon66@hanmail.net
Kim
K S
KS
Hong
S G
SG
Kang
B J
BJ
Lee
M Y
MY
Cho
D W
DW
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D053938:DNA Fragmentation; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007031:Hypothalamus / Q000187:drug effects*; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011419:Propidium / Q000494:pharmacology; D012995:Solubility
D004396:Coloring Agents; D016877:Oxidants; D010936:Plant Extracts; D011419:Propidium; D006861:Hydrogen Peroxide