Molecular cloning and characterization of human MAWD, a novel protein containing WD-40 repeats frequently overexpressed in breast cancer.
A full-length cDNA clone encoding a novel protein containing WD-40 repeats, which were frequently involved in protein-protein interactions, was isolated and sequenced. This clone had a predicted open reading frame (ORF) encoding 350 amino acids possessing six repeats of WD-40 motif. It was most closely homologous to TRIP-1, a phosphorylation substrate of the transforming growth factor-beta type II receptor. In the process of characterizing the function of the new gene product, we found that overexpression of the gene seemed to activate mitogen-activated protein kinase and to promote anchorage-independent growth of the cells. Moreover, the gene product was frequently overexpressed in human tumor breast tissues compared with their normal breast tissues, suggesting that the gene might be involved in the tumor progression. Radiation hybrid mapping placed the gene into human chromosome 12q11-12 near the marker D12S1593.
Matsuda
S
S
Department of Molecular Pathogenesis, Nagoya University School of Medicine, Japan. smatsuda@tsuru.med.nagoya-u.ac.jp
Katsumata
R
R
Okuda
T
T
Yamamoto
T
T
Miyazaki
K
K
Senga
T
T
Machida
K
K
Thant
A A
AA
Nakatsugawa
S
S
Hamaguchi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism; D019556:COS Cells; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry* / Q000378:metabolism; D003001:Cloning, Molecular; D004789:Enzyme Activation; D039621:Eukaryotic Initiation Factor-3; D005260:Female; D006801:Humans; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D039621:Eukaryotic Initiation Factor-3; D009363:Neoplasm Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; C403447:STRAP protein, human; C096013:EIF3I protein, human; D020929:Mitogen-Activated Protein Kinase Kinases