title: Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7.
abstract: 8 h) and high steady-state levels of protein accumulation, while the H2 intrabodies had a half-life of 2 h and less protein at steady state. These results suggest that the choice of sFv as an intrabody depends critically on the intracellular sFv protein having an extended half-life and elevated steady-state level. Thus, extended half-life must be considered together with sFv antibody specificity and affinity when choosing an optimal sFv intrabody for functional studies of cellular proteins.
authors.affiliation: IntraImmune Therapies Inc., P.O. Box 15599, Boston, MA 02215-0011, USA.quanzhu@tiac.net
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.forename: C
authors.initials: C
authors.lastname: Zeng
authors.forename: A
authors.initials: A
authors.lastname: Huhalov
authors.forename: J
authors.initials: J
authors.lastname: Yao
authors.forename: T G
authors.initials: TG
authors.lastname: Turi
authors.forename: D
authors.initials: D
authors.lastname: Danley
authors.forename: T
authors.initials: T
authors.lastname: Hynes
authors.forename: Y
authors.initials: Y
authors.lastname: Cong
authors.forename: D
authors.initials: D
authors.lastname: DiMattia
authors.forename: S
authors.initials: S
authors.lastname: Kennedy
authors.forename: G
authors.initials: G
authors.lastname: Daumy
authors.forename: E
authors.initials: E
authors.lastname: Schaeffer
authors.forename: W A
authors.initials: WA
authors.lastname: Marasco
authors.forename: J S
authors.initials: JS
authors.lastname: Huston
mesh_terms: D000818:Animals; D017209:Apoptosis; D053179:Caspase 7; D020169:Caspases / Q000276:immunology*; D002460:Cell Line; D002461:Cell Line, Transformed; D002467:Cell Nucleus; D006224:Cricetinae; D003546:Cysteine Endopeptidases / Q000276:immunology*; D015870:Gene Expression; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D019169:Jurkat Cells; D014162:Transfection
chemical_list: D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; C505520:CASP7 protein, human; D053179:Caspase 7; D020169:Caspases; D003546:Cysteine Endopeptidases