Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator.
Acute, low-dose ultraviolet B radiation (UVR) alters cutaneous immunity at the local site as well as systemically. Within 2-3 days of UVR exposure, recipient mice lose their capacity to develop contact hypersensitivity (CH) when hapten is painted on unexposed skin. This loss correlates temporally with a functional deficit among dendritic antigen-presenting cells within non-draining lymph nodes and spleen. In the experiments described, the delayed systemic immune deficiency following acute, low-dose UVR exposure was found to be eliminated with neutralizing anti-interleukin-10 (IL-10) antibody. Intracutaneous injection of IL-10 generated a deficiency of systemic immunity as well as a functional deficit among lymph node dendritic cells that was similar to that induced by UVR. The skin itself was found to be the source of the IL-10 responsible for these defects, and epidermis (presumably keratinocytes) rather than mast cells was found to be the source of IL-10 within UVR-exposed skin. The potential relationships are discussed between the delayed systemic immune deficit created by acute, low-dose UVR, and the systemic immune deficits caused by chronic, high-dose UVR and by a single, high-dose UVR exposure.
Kurimoto
I
I
Schepens Eye Research Institute and Department of Dermatology, Harvard Medical School, Boston, MA 02114, USA.
Kitazawa
T
T
Streilein
J W
JW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D003877:Dermatitis, Contact / Q000276:immunology*; D004139:Dinitrofluorobenzene; D004307:Dose-Response Relationship, Radiation; D004817:Epidermis / Q000276:immunology* / Q000528:radiation effects; D007109:Immunity / Q000528:radiation effects*; D016753:Interleukin-10 / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D007509:Irritants; D015603:Keratinocytes / Q000276:immunology; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D013997:Time Factors; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000911:Antibodies, Monoclonal; D007509:Irritants; D016753:Interleukin-10; D004139:Dinitrofluorobenzene