[Changes in the function of the nucleus and chromatin of human cells under the action of hormonal factors in vitro].
The influence of hormones--adrenaline, noradrenaline, hydrocortisone on the electronegativity of cell nuclei (ENN) was shown by the method of intracellular microelectrophoresis. Changes in heterochromatin state were estimated on the basis of heterochromatin granules (chromocenters) quantity. Adrenaline and noradrenaline induced the decrease in ENN, the maximum effect was revealed with hormones concentration observed in human blood during physical training. The hormonal effect increased with gradual increase of the exposition time from 5 to 120 min. Hydrocortisone also decreased ENN. Adrenaline, and in some cases hydrocortisone, induced the significant increase of heterochromatin granule quantity. The correlation of this process with ENN decrease was shown only in the case of adrenaline. Obtained data are discussed in connection with regulation mechanisms of cell nucleus functional activity.
Shkorbatov
Iu G
IuG
Shakhbazov
V G
VG
Gorenskaia
O V
OV
Dmitruk
T V
TV
Montvid
P Iu
PIu
D000293:Adolescent; D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D002610:Cheek; D002843:Chromatin / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D004837:Epinephrine / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects; D008875:Middle Aged; D009638:Norepinephrine / Q000494:pharmacology*; D013997:Time Factors
D002843:Chromatin; D006854:Hydrocortisone; D009638:Norepinephrine; D004837:Epinephrine