title: Broadening the Aldolase Catalytic Antibody Repertoire by Combining Reactive Immunization and Transition State Theory: New Enantio- and Diastereoselectivities.
abstract: Nine efficient aldolase antibodies were generated by using hapten 1. This hapten unites reactive immunization and the transition state analogue approach in a single molecule. Characterization of two of these antibodies reveals that they are highly proficient (up to 1000-fold better than any other antibody catalyst) and enantioselective catalysts for aldol and retro-aldol reactions and exhibit enantio- and diastereoselectivities opposite to that of antibody 38C2.
authors.affiliation: The Skaggs Institute for Chemical Biology and, The Department of Molecular Biology, The Scripps Research Institute, 10 550, North Torrey Pines Road, La Jolla, CA 92037 (USA).
authors.initials: G
authors.lastname: Zhong
authors.initials: RA
authors.lastname: Lerner
authors.initials: III
authors.lastname: Barbas