Leukocyte adhesion molecule expression and T cell naïve/memory status following isoproterenol infusion.
This study examined adhesion molecules on peripheral leukocytes following a 30-min infusion of the beta-adrenergic agonist isoproterenol in 23 healthy subjects. In response to isoproterenol, the number of CD8 +CD62L- T cells and both CD62L+ and CD62L-natural killer (NK) (CD3 CD16+ 56+) cells increased markedly in circulation (p < 0.001). In addition, the surface density of CD62L was significantly lower on both CD8+ and CD4+ T cells (p < 0.001). Plasma levels of soluble CD62L remained unchanged, arguing against an isoproterenol-induced shedding of L-selectin. In contrast to CD62L, the surface density of the beta2 integrin LFA-1 (CD11a) was higher on circulating lymphocytes (p < 0.001) (but not monocytes or lymphocytes) post-infusion. Isoproterenol also led to a mobilization of memory/activated CD8+CD29high T cells (p < 0.01), but had no significant effect on the number of circulating CD8+ CD45RA+ CD62L+ naïve T cells. beta blockade with the non-specific antagonist propranolol eliminated these isoproterenol-induced effects.
Mills
P J
PJ
Department of Psychiatry, UCSD Medical Center, San Diego, CA 92103-0804, USA. pmills@ucsd.edu
Goebel
M
M
Rehman
J
J
Irwin
M R
MR
Maisel
A S
AS
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000328:Adult; D001773:Blood Cells / Q000276:immunology; D018821:CD18 Antigens / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D019012:Integrin beta1 / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007545:Isoproterenol / Q000494:pharmacology*; D019041:L-Selectin / Q000032:analysis; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*
D000318:Adrenergic beta-Agonists; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D019012:Integrin beta1; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D007545:Isoproterenol