title: [Interaction of 3'-fluoro-3'-deoxyanalogs of a trimer of (2'-5')oligoadenylic acid with (2'-5')A3-antibodies: stereochemical aspect].
abstract: Mouse antibodies to (2'-5')oligoadenylates were obtained by the immunization of animals with the (2'-5')oligoadenylic acid trimer conjugated with bovine serum albumin through a 2',3'-levulinic acid residue. Using radioimmunoassay, the reactivity of mouse polyclonal antibodies to the (2'-5')oligoadenylic acid trimer was studied for the trimer analogues containing 9-(3-deoxy-3-fluro-beta-D- xylofuranosyl)adenine and 3'-deoxy-3'-fluoro-adenosine in various positions of the chain. It was found that (a) the three-dimensional structure of short oligonucleotides is an important factor in the antibody recognition; (b) antibodies are more sensitive to modifications of the 5'-terminal and central ribose fragments of the (2'-5')oligoadenylic acid trimer; (c) the 3'-hydroxyl group plays a secondary role in the formation of the antigen determinant.
authors.affiliation: Institute of Bioorganic Chemistry, Belarussian Academy of Sciences, Minsk, Belarus.
authors.forename: E N
authors.initials: EN
authors.lastname: Kalinichenko
authors.forename: T L
authors.initials: TL
authors.lastname: Podkopaeva
authors.forename: I A
authors.initials: IA
authors.lastname: Mikhaĭlopulo
authors.forename: M
authors.initials: M
authors.lastname: Kelve
authors.forename: M
authors.initials: M
authors.lastname: Saarma
authors.forename: J
authors.initials: J
authors.lastname: van den Boogaart
authors.forename: C
authors.initials: C
authors.lastname: Altona
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D001704:Biopolymers; D002942:Circular Dichroism; D051379:Mice; D009843:Oligoribonucleotides / Q000737:chemistry / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology; D013237:Stereoisomerism
chemical_list: D000227:Adenine Nucleotides; D000906:Antibodies; D001704:Biopolymers; D009843:Oligoribonucleotides; D012710:Serum Albumin, Bovine; C023505:2',5'-oligoadenylate