title: Relationship between cancellous bone induced magnetic field and ultrastructure in a rat ovariectomy model.
abstract: The site-dependent variations in trabecular bone morphology were studied in the rat tibia by magnitude and phase difference three-dimensional nuclear magnetic resonance microscopy and image processing, and the implications of ovariectomy were evaluated. Specimens excised from the proximal tibial metaphysis in ovariectomized (n = 7) and intact control (n = 4) rats were imaged at 9.4T with their anatomic axes parallel to the direction of the magnetic field. An echo-offset 3D rapid spin-echo excitation pulse sequence was used to generate phase difference maps, from which the standard deviation of the phase difference, sigma(delta psi), was calculated. In addition, a fictitious rate constant, R2', was calculated from the slope of the exponential portion of the Fourier transform of the phase difference histogram. Trabecular bone volume fraction was also determined in the same volume of interest. The results show strong correlations between bone volume fraction and both sigma(delta psi) and R2', suggesting that these parameters could be useful for nondestructive assessment of trabecular bone volume.
authors.affiliation: Department of Radiology, University of Pennsylvania Medical Center, Philadelphia, USA.
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: F W
authors.initials: FW
authors.lastname: Wehrli
authors.forename: S N
authors.initials: SN
authors.lastname: Hwang
authors.forename: S L
authors.initials: SL
authors.lastname: Wehrli
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004574:Electromagnetic Fields; D005260:Female; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D009682:Magnetic Resonance Spectroscopy; D010024:Osteoporosis / Q000175:diagnosis* / Q000209:etiology; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000648:ultrastructure*