Identification of the bright-greenish-yellow-fluorescence (BGY-F) compound on cotton lint associated with aflatoxin contamination in cottonseed.
In order to characterize the structure of the bright-greenish-yellow-fluorescence (BGY-F) compound on cotton lint associated with aflatoxin contamination in cotton seed, various in vitro and in vivo natural BGY-F reaction products were prepared. Under similar high pressure liquid chromatography separation with variable wavelength and programmable fluorescence detection (HPLC-UV/FL), combined with atmospheric pressure ionization and mass spectral determinations it was found that the BGY-F reaction products prepared from three preparations: (a) kojic acid (KA) + peroxidase (soybean peroxide or horseradish type VI and type II) + H2O2, or (b) detached fresh cotton locules + KA + H2O2, or (c) attached field cotton locules that were treated with a spore suspension of aflatoxigenic Aspergillus flavus, all resulted in identical chromatographic characteristics, and all exhibited a molecular weight of 282. Further characterization of the BGY-F reaction product with 1H- and 13C-NMR spectroscopic analysis revealed that it was a dehydrogenator dimer of 2 KA, linked through the C-6 positions.
Zeringue
H J
HJ
U.S. Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70179-0687, USA. zeringue@nola.srrc.usda.gov
Shih
B Y
BY
Maskos
K
K
Grimm
D
D
D000348:Aflatoxins / Q000032:analysis*; D001231:Aspergillus flavus; D002851:Chromatography, High Pressure Liquid; D003368:Gossypium / Q000737:chemistry* / Q000382:microbiology*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D011753:Pyrones / Q000032:analysis* / Q000737:chemistry; D012639:Seeds / Q000737:chemistry* / Q000382:microbiology; D013050:Spectrometry, Fluorescence
D000348:Aflatoxins; C404043:BGY-F compound; D007202:Indicators and Reagents; D011753:Pyrones