title: Ozone potentiates vitamin E depletion by ultraviolet radiation in the murine stratum corneum.
abstract: As the outermost layer of the skin, the stratum corneum is exposed to environmental oxidants. To investigate putative synergisms of environmental oxidative stressors in stratum corneum, hairless mice were exposed to ultraviolet radiation (UV) and ozone (O(3)) alone and in combination. Whereas a significant depletion of alpha-tocopherol was observed after individual exposure to either a 0.5 minimal erythemal dose of UV or 1 ppm O(3) for 2 h, the combination did not increase the effect of UV alone. However, a dose of 0.5 ppm O(3) x 2 h, which had no effect when used alone, significantly enhanced the UV-induced depletion of vitamin E. We conclude that concomitant exposure to low doses of UV and O(3) at levels near those that humans can be exposed to causes additive oxidative stress in the stratum corneum.
authors.affiliation: Institute of General Physiology, University of Siena, Siena, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Valacchi
authors.forename: S U
authors.initials: SU
authors.lastname: Weber
authors.forename: C
authors.initials: C
authors.lastname: Luu
authors.forename: C E
authors.initials: CE
authors.lastname: Cross
authors.forename: L
authors.initials: L
authors.lastname: Packer
mesh_terms: D000818:Animals; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008812:Mice, Hairless; D010083:Oxidants, Photochemical / Q000633:toxicity*; D018384:Oxidative Stress; D010126:Ozone / Q000633:toxicity*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014810:Vitamin E / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000209:etiology / Q000378:metabolism
chemical_list: D010083:Oxidants, Photochemical; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D010126:Ozone