title: Vascular smooth muscle differentiation of murine stroma: a sequential model.
abstract: Previous studies by our group showed that stromal cells from human long-term marrow cultures were mesenchymal cells following a vascular smooth muscle pathway. The present study using 58 immortalized stromal lines from different hematopoietic sites was conducted to verify whether this hypothesis also held true for murine stroma. Principal components analysis performed using cytoskeletal and extracellular matrix proteins allowed the segregation of five factors explaining more than 70% of the variance. Factor I, including osteopontin and vimentin, and factor II, laminins and fibronectins, were representative of the mesenchyme. The remaining three factors were representative of vascular smooth muscle: factor III, including alphaSM actin, SM alpha actinin, SM22alpha, EDa+ fibronectin, and thrombospondin-1; factor IV, metavinculin and h-caldesmon; and factor V, smooth muscle myosin SM1 and desmin. All lines expressed factors I and II; 53 lines expressed factor III, 35 lines expressed factor IV; and 11 lines expressed factor V. A second principal components analysis including membrane antigens indicated the cosegregration of vascular cell adhesion molecule-1 with osteopontin and that of Ly6A/E with vimentin, whereas CD34 and Thy-1 appeared to be independent factors. The heterogeneity of vascular smooth muscle markers expression suggests that harmonious maintenance of hematopoiesis depends on the cooperation between different stromal cell clones.
authors.affiliation: E.T.S. de Franche-Comté and Departement d'Information Medicale, Besancon, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Remy-Martin
authors.forename: A
authors.initials: A
authors.lastname: Marandin
authors.forename: B
authors.initials: B
authors.lastname: Challier
authors.forename: G
authors.initials: G
authors.lastname: Bernard
authors.forename: M
authors.initials: M
authors.lastname: Deschaseaux
authors.forename: P
authors.initials: P
authors.lastname: Herve
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: R
authors.initials: R
authors.lastname: Auerbach
authors.forename: J E
authors.initials: JE
authors.lastname: Dennis
authors.forename: K A
authors.initials: KA
authors.lastname: Moore
authors.forename: J S
authors.initials: JS
authors.lastname: Greenberger
authors.forename: P
authors.initials: P
authors.lastname: Charbord
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D006410:Hematopoiesis; D006801:Humans; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins