title: Inositol 1,4,5-trisphosphate receptor associated with focal contact cytoskeletal proteins.
abstract: The linkage between inositol 1,4,5-trisphosphate (IP(3)) receptors (IP(3)Rs) and cytoskeletal proteins is considered to be important in cell function. In the present study, the association of IP(3)R subtypes with cytoskeletal proteins was examined using monoclonal antibodies specific to each IP(3)R subtype. We found that IP(3)R type 2 colocalized with talin, a focal contact cytoskeletal protein. IP(3)R type 2 exhibited a patchy distribution in the peripheral cytoplasm differently from type 1 and type 3 IP(3)R. Furthermore, IP(3)R subtypes co-immunoprecipitated with talin, vinculin and alpha-actin, but not alpha-actinin or paxillin.
authors.affiliation: Vessel Research Laboratory Co., Ltd., 3-6-6 Asahimachi, Machida-shi, Tokyo, Japan. sugiyama@hri.co.jp
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Mikoshiba
mesh_terms: D000185:Actinin / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D015220:Calcium Channels / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000378:metabolism; D051419:Paxillin; D010750:Phosphoproteins / Q000378:metabolism; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000145:classification / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D016608:Talin / Q000378:metabolism; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D015220:Calcium Channels; D003598:Cytoskeletal Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051419:Paxillin; D010750:Phosphoproteins; C496062:Pxn protein, mouse; C496063:Pxn protein, rat; D018160:Receptors, Cytoplasmic and Nuclear; D016608:Talin; D000185:Actinin; D016596:Vinculin; D015544:Inositol 1,4,5-Trisphosphate