Identification and characterization of the point mutation which affects the transcription level of the chromosomal 3-N-acetyltransferase gene of Streptomyces griseus SS-1198.
We determined the molecular basis for the enhanced expression of the aac(3)-Xa gene encoding an aminoglycoside 3-N-acetyltransferase in Streptomyces griseus. A C-->T substitution was identified at the putative promoter of the mutant gene. RNA analyses demonstrated that the substitution caused a marked increase in the production of the gene-specific transcripts. Therefore, it seemed very likely that the aac(3)-Xa gene was activated by the substitution resulting in the emergence of a stronger promoter.
Ishikawa
J
J
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, Japan. jun@nih.go.jp
Sunada
A
A
Oyama
R
R
Hotta
K
K
D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D001483:Base Sequence; D002875:Chromosomes; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D017354:Point Mutation; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012333:RNA, Messenger; D001191:Arylamine N-Acetyltransferase