Structure of the O-specific polysaccharide of Proteus penneri 71 and classification of cross-reactive P. penneri strains to a new proposed serogroup O64.
A neutral O-specific polysaccharide (O-antigen) was isolated from the lipopolysaccharide (LPS) of the bacterium Proteus penneri 71. On the basis of sugar analysis and 1H- and 13C-NMR spectroscopic studies, including two-dimensional COSY, 13C,1H heteronuclear COSY and ROESY, the following structure of the trisaccharide repeating unit of the polysaccharide was established: -->3)-beta-D-GlcpNAc-(1-->4)-beta-D-GlcpNAc-(1-->3)-alpha-D-Galp-(1-- > The polysaccharide has the same carbohydrate backbone as the O-specific polysaccharide of P. penneri 19 and both are similar to that of P. penneri 62 studied by us previously. A cross-reactivity of anti-P. penneri 71, 19 and 62 O-antisera with 11 P. penneri strains was revealed and substantiated at the level of the O-antigen structures. These strains could be divided into three subgroups within a new proposed Proteus O64 serogroup containing P. penneri strains only.
Zych
K
K
Department of General Microbiology, Institute of Microbiology and Immunology, University of Lódz, Poland.
Kocharova
N A
NA
Kowalczyk
M
M
Toukach
F V
FV
Kaminska
D
D
Shashkov
A S
AS
Knirel
Y A
YA
Sidorczyk
Z
Z
D000818:Animals; D000907:Antibodies, Bacterial; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011511:Proteus / Q000737:chemistry* / Q000145:classification / Q000276:immunology*; D011817:Rabbits; D012703:Serotyping; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000939:Epitopes; D019081:O Antigens