title: Pharmacokinetics of bisphenol A released from a dental sealant.
abstract: BACKGROUND
  Limited information is available regarding potentially estrogenic bisphenol A, or BPA, released from dental sealants. This study determined the rate- and time-course of BPA released from a dental sealant (Delton Opaque Light-cure Pit and Fissure Sealant, Preventive Care/Dentsply) when applied at a dosage of 8 milligrams (one tooth) or 32 mg (8 mg on each of four teeth) to 40 healthy adults.
  
  
  METHODS
  The authors recruited 40 healthy subjects (18 men and 22 women, 20-55 years of age) who did not have histories of pit and fissure sealant placement or composite resin restorations. The authors collected saliva (30 milliliters) and blood (7 mL) specimens from all subjects immediately before sealant placement (baseline) and at one hour, three hours, one day, three days and five days after sealant placement. They used high-pressure liquid chromatography to determine BPA (detection sensitivity 5 parts per billion, or ppb) in all specimens.
  
  
  RESULTS
  The authors detected BPA in some saliva specimens (5.8-105.6 ppb) collected at one hour and three hours. The BPA, however, was not detectable beyond three hours or in any of the serum specimens. For the one- and three-hour saliva samples, the BPA concentration in the high-dose (32 mg) group was significantly greater than in the low-dose (8 mg) group (P < .05, Wilcoxon signed rank test). In the high-dose group, there was a significant decrease in saliva BPA concentrations from one hour to three hours (P < .01, Wilcoxon signed rank test).
  
  
  CONCLUSIONS
  This study showed that BPA released orally from a dental sealant may not be absorbed or may be present in nondetectable amounts in systemic circulation. The concern about potential estrogenicity of sealant may be unfounded.
authors.affiliation: Department of Oral Biology, University of Nebraska Medical Center, College of Dentistry, Lincoln 68583-0704, USA.
authors.forename: E Y
authors.initials: EY
authors.lastname: Fung
authors.forename: N O
authors.initials: NO
authors.lastname: Ewoldsen
authors.forename: H A
authors.initials: HA
authors.lastname: St Germain
authors.forename: D B
authors.initials: DB
authors.lastname: Marx
authors.forename: C L
authors.initials: CL
authors.lastname: Miaw
authors.forename: C
authors.initials: C
authors.lastname: Siew
authors.forename: H N
authors.initials: HN
authors.lastname: Chou
authors.forename: S E
authors.initials: SE
authors.lastname: Gruninger
authors.forename: D M
authors.initials: DM
authors.lastname: Meyer
mesh_terms: D000328:Adult; D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate / Q000032:analysis* / Q000737:chemistry; D001822:Body Burden; D002851:Chromatography, High Pressure Liquid; D004968:Estrogens, Non-Steroidal / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010636:Phenols / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D010895:Pit and Fissure Sealants / Q000032:analysis* / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D010895:Pit and Fissure Sealants; C006780:bisphenol A; D000098826:Bisphenol A Compounds