Cloning, expression, and chromosomal localization of a mouse gene homologous to the germ cell migration regulator wunen and to type 2 phosphatidic acid phosphatases.
The wunen gene of Drosophila melanogaster encodes a multipass membrane-spanning protein that negatively regulates primordial germ cell migration. Here we describe the cloning of a mouse gene that encodes a protein homologous to wunen and to the Type 2 phosphatidic acid phosphatases. This gene encodes a 251-amino-acid protein that most closely resembles the human Type 2 phosphatidic acid phosphatase PAP-2c. Northern blot analysis revealed the presence of a single 1.9-kb Ppap2c transcript. The Ppap2c gene was localized to the central portion of mouse Chromosome 10 by interspecific backcross analysis.
Zhang
N
N
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Gridley
T
T
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002465:Cell Movement / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D005854:Germ Cells; D006801:Humans; D019476:Insect Proteins / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010711:Phosphatidate Phosphatase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; C406242:phosphatidic acid phosphatase type 2; D010711:Phosphatidate Phosphatase; C103065:wun protein, Drosophila