title: Structure-related aspects on water diffusivity in fatty acid-soap and skin lipid model systems.
abstract: Simplified skin barrier models are necessary to get a first hand understanding of the very complex morphology and physical properties of the human skin barrier. In addition, it is of great importance to construct relevant models that will allow for rational testing of barrier perturbing/occlusive effects of a large variety of substances. The primary objective of this work was to study the effect of lipid morphology on water permeation through various lipid mixtures (i.e., partly neutralised free fatty acids, as well as a skin lipid model mixture). In addition, the effects of incorporating Azone((R)) (1-dodecyl-azacycloheptan-2-one) into the skin lipid model mixture was studied. Small- and wide-angle X-ray diffraction was used for structure determinations. It is concluded that: (a) the water flux through a crystalline fatty acid-sodium soap-water mixture (s) is statistically significantly higher than the water flux through the corresponding lamellar (L(alpha)) and reversed hexagonal (H(II)) liquid crystalline phases, which do not differ between themselves; (b) the water flux through mixtures of L(alpha)/s decreases statistically significantly with increasing relative amounts of lamellar (L(alpha)) liquid crystalline phase; (c) the addition of Azone((R)) to a skin lipid model system induces a reduction in water flux. However, further studies are needed to more closely characterise the structural basis for the occlusive effects of Azone((R)) on water flux.
authors.affiliation: Department of Biochemistry and Biophysics, Karolinska Institute, S-171 77, Stockholm, Sweden. LPO.Norlen@mbb.ki.se
authors.forename: L
authors.initials: L
authors.lastname: Norlén
authors.forename: J
authors.initials: J
authors.lastname: Engblom
mesh_terms: D001381:Azepines / Q000378:metabolism / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism; D003460:Crystallization; D004058:Diffusion; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008953:Models, Anatomic; D012867:Skin / Q000033:anatomy & histology / Q000378:metabolism*; D012869:Skin Absorption; D012915:Soaps; D013329:Structure-Activity Relationship; D014867:Water / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D001381:Azepines; D005230:Fatty Acids, Nonesterified; D006028:Glycosphingolipids; D012915:Soaps; D014867:Water; C035229:laurocapram; C018480:ceramide 3; D002784:Cholesterol