Salmonella typhimurium forms adenylcobamide and 2-methyladenylcobamide, but no detectable cobalamin during strictly anaerobic growth.
Under microaerophilic conditions Salmonella typhimurium LT2 synthesizes cobalamin, during which 5,6-dimethylbenzimidazole is formed from riboflavin. We report here that in an anoxic environment S. typhimurium did not form cobalamin, but rather adenylcobamide, 2-methyladenylcobamide, and cobyric acid. This indicated that S. typhimurium, like other microorganisms that synthesize 5,6-dimethylbenzimidazole from riboflavin, requires oxygen for the formation of the cobalamin base.
Keck
B
B
Institut für Biologische Chemie und Ernährungswissenschaft, Universität-Hohenheim, Stuttgart, Germany.
Renz
P
P
D000693:Anaerobiosis; D003038:Cobamides / Q000378:metabolism*; D012256:Riboflavin / Q000378:metabolism; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism*; D014805:Vitamin B 12 / Q000378:metabolism*
D003038:Cobamides; D014805:Vitamin B 12; D012256:Riboflavin