gammadelta T cells lyse autologous and allogenic oesophageal tumours: involvement of heat-shock proteins in the tumour cell lysis.
T cells expressing gammadelta receptors were isolated from the peripheral blood of oesophageal cancer patients and analysed for their potential to lyse tumour targets. Immunophenotyping by flow cytometry showed that the dominant population of gammadelta T cells expressed the Vgamma9 and the Vdelta2 T cell receptor, and a minor population expressed the Vdelta1 receptor. Cytotoxicity assays revealed that activated gammadelta T cells lysed Daudi Burkitt's lymphoma and K562 cells. Lysis of autologous oesophageal tumours was higher than of allogenic tumours. Anti-hsp60 and anti-hsp70 mAb significantly inhibited the cytotoxicity of gammadelta T cells to both autologous and allogenic oesophageal tumours. Surface expression of hsp60 and hsp70 on oesophageal tumours and Daudi cells was demonstrated by flow cytometry. In conclusion, gammadelta T cells isolated from the peripheral blood of oesophageal cancer patients have the ability of kill oesophageal tumour cells. The lysis of tumour targets by the gammadelta T cells is brought about via recognition of heat-shock proteins expressed on the surface of tumour cells. gammadelta T cells isolated from the peripheral blood may have applications in adoptive immunotherapy of oesophageal cancer.
Thomas
M L
ML
Cellular Immunology Unit, Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai-400012, India.
Samant
U C
UC
Deshpande
R K
RK
Chiplunkar
S V
SV
D000951:Antigens, Neoplasm / Q000276:immunology*; D002051:Burkitt Lymphoma / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000276:immunology* / Q000473:pathology; D018834:Chaperonin 60 / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D004938:Esophageal Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D020014:K562 Cells; D009363:Neoplasm Proteins / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D009363:Neoplasm Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta