title: [Cloning and sequence analysis of ATCase genes from Psychrophilic vibrio].
abstract: The gene encoding for aspartate transcarbamoylase (ATCase) from Psychrophilic vibrio, strain 2693 was cloned and sequenced. The sequence revealed the existence of two gene encoding respectively for a catalytic chain (pyrB) and a regulatory chain (pyr I). The catalytic and regulatory polypeptide chains of Vibrio 2693 ATCase are encoded by a single pyrBI bicistronic operon, and appear to be transcribed under the control of the same promoter. The 3'-terminus of the catalytic cistron (pyrB) is adjacent to the 5'-terminus of the regulatory cistron, these is only a 4 bp space between the two coding regions.
authors.affiliation: Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
authors.forename: Y F
authors.initials: YF
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: M V
authors.initials: MV
authors.lastname: de Casteele
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Liang
authors.forename: N
authors.initials: N
authors.lastname: Glansdorff
mesh_terms: D000595:Amino Acid Sequence; D001221:Aspartate Carbamoyltransferase / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D014733:Vibrio / Q000201:enzymology / Q000235:genetics*
chemical_list: D001221:Aspartate Carbamoyltransferase