Mass spectrometric identification and high-performance liquid chromatographic determination of a flavonoid glycoside naringin in human urine.
The aim of this study was to evaluate the absorption of a citrus flavonoid, naringin, as its glycosylated form. Six healthy volunteers (three males and three females) were studied. After a single oral administeration of 500 mg of naringin, intact naringin was isolated from 2-4 h urine. Isolated naringin was identified by the LC/electrospray ionization mass spectrometry (ESI-MS), MS/MS, and MS/MS/MS techniques. The cumulative urinary excretion of naringin and its metabolites (naringenin and naringenin glucuronides) was determined by HPLC for 0-24 h. Approximately 0.02% of the administered dose was recovered in urine as unchanged naringin, whereas urinary recoveries of naringenin and naringenin glucuronides were approximately 0.4 and 3.6% of the administered dose, respectively. It was concluded that trace amounts of orally administered naringin can be absorbed as the glycoside. However, it is not clear whether the glycoside is cleaved before or after absorption to generate naringenin.
Ishii
K
K
School of Health Sciences, Kyorin University, 476 Miyasita, Hachioji, Tokyo 192-8508, Japan.
Furuta
T
T
Kasuya
Y
Y
D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D044950:Flavanones; D005419:Flavonoids / Q000652:urine*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry
D000975:Antioxidants; D044950:Flavanones; D005419:Flavonoids; C005274:naringin