Aluminum toxicity. Hematological effects.
Sequential effects of intoxication with aluminum hydroxide (Al) (80 mg/Kg body weight, i.p., three times a week), were studied on rats from weaning and up to 28 weeks. The study was carried out on hematological and iron metabolism-related parameters on peripheral blood, at the end of the 1st, 2nd, 3rd, 4th, 5th and 6th months of exposure. As it was described that hematotoxic effects of Al are mainly seen together with high levels of uremia, renal function was measured at the same periods. The animals treated developed a microcytosis and was accompanied by a decrease in mean corpuscular hemoglobin (MCH). Significantly lower red blood cell counts (RBC million/microl) were found in rats treated during the 1st month. These values matched those obtained for control rats during the 2nd month. From the 3rd month onwards, a significant increase was observed as compared to control groups, and the following values were obtained by the 6th month: (T) 10.0 +/- 0.3 versus (C) 8.7 +/- 0.2 (million/microl). Both MCH and mean corpuscular volume (MCV) were found to be significantly lower in groups treated from the 2nd month. At the end of the 6th month the following values were found: MCH (T) 13.3 +/- 0.1 versus (C) 16.9 +/- 0.3 (pg); MCV (T) 42.1 +/- 0.7 versus (C) 51.8 +/- 0.9 (fl). Al was found responsible for lower serum iron concentration levels and in the percentage of transferrin saturation. Thus, although microcytic anemia constitutes an evidence of chronic aluminum exposure, prolonged exposure could lead to a recovery of hematocrit and hemoglobin concentration values with an increase in red cell number. Nevertheless, both microcytosis and the decrease of MCH would persist. These modifications took place without changes being observed in the renal function during the observation period.
Mahieu
S
S
Cátedra de Fisiología Humana, Facultad de Bioquímica y de Ciencas Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, Argentina. smahieu@fbcb.unl.edu.ar
del Carmen Contini
M
M
Gonzalez
M
M
Millen
N
N
Elias
M M
MM
D000536:Aluminum Hydroxide / Q000097:blood / Q000633:toxicity*; D000818:Animals; D001769:Blood / Q000187:drug effects*; D004032:Diet; D004906:Erythrocyte Count / Q000187:drug effects; D004909:Erythrocyte Indices / Q000187:drug effects; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007501:Iron / Q000097:blood / Q000378:metabolism; D007668:Kidney / Q000187:drug effects; D007677:Kidney Function Tests; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D006454:Hemoglobins; D000536:Aluminum Hydroxide; D007501:Iron