Traditional and emerging methods for analyzing cell activity in cell culture.
The selection of appropriate techniques to assay for markers of cell activity is important for obtaining optimal results in cell culture-based research. This paper is intended as a guide to many of the assays currently available and new techniques that have been recently introduced in the literature. This paper addresses both manual assay techniques, including the use of hemocytometers, phase contrast microscopy, cell staining, and the immunofluorescent antibody assay (IFA), and automated assays for cell activity, including stained optical density, proliferating cell nuclear antigen, creatine kinase assay, DNA quantification, electronic cell counting, flow cytometry, magnetic cell sorting, image analysis, chemiluminescence, radioisotope labeling, precursor incorporation, in-situ hybridization/ligand binding, and enzyme-linked immuno-culture assay (ELICA). Advantages/disadvantages and applicability of these assays to different areas of cell culture research are discussed, and guidelines for selecting an appropriate assay are suggested.
Stewart
N T
NT
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA, USA.
Byrne
K M
KM
Hosick
H L
HL
Vierck
J L
JL
Dodson
M V
MV
D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured / Q000378:metabolism; D004247:DNA / Q000032:analysis; D004798:Enzymes / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D018189:Immunomagnetic Separation; D017403:In Situ Hybridization
D004798:Enzymes; D004247:DNA