Role of an oxidative stress in the macrophage dysfunction caused by erythrophagocytosis.
A phagocytic challenge with immunoglobulin G (IgG)-coated erythrocytes (EIgGs) has been shown to cause a subsequent depression of macrophage respiratory burst capacity and phagocytic function. The present study evaluated the hypothesis that this macrophage dysfunction is caused by an oxidative stress. An oxidative stress induced by ferric ammonium citrate (FAC) plus cumene hydroperoxide (CHP) caused a depression of macrophage function that was attenuated by antioxidants and iron chelators. In contrast, the same antioxidants and iron chelators did not alter changes caused by a challenge with EIgGs. EIgG challenge caused an increase in lipid peroxidation but failed to deplete glutathione (GSH) or decrease the activity of glyceraldehyde-3-phosphate dehydrogenase (GA-3-PD), suggesting that there was only a slight oxidative stress. Inhibition of the Fc gamma receptor (Fc gammaR) stimulated respiratory burst by removing calcium during the challenge did not attenuate the changes caused by an EIgG challenge. A phagocytic challenge with nonerythrocyte particles, IgG-coated beads (BIgGs), did not depress the respiratory burst capacity but did depress phagocytic function. Fc gammaR expression was depressed following a phagocytic challenge but not an oxidative stress. Thus, an oxidative stress can depress macrophage function, but the dysfunction caused by a phagocytic challenge with EIgGs involves Fc gammaR depletion and the erythrocyte contents rather than an oxidative stress.
Raley
M J
MJ
Department of Basic and Pharmaceutical Science, Albany Medical College, NY 12208-3479, USA.
Loegering
D J
DJ
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001555:Benzene Derivatives / Q000494:pharmacology; D002460:Cell Line; D004912:Erythrocytes / Q000276:immunology; D005290:Ferric Compounds / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007502:Iron Chelating Agents / Q000494:pharmacology; D015227:Lipid Peroxidation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D010587:Phagocytosis; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D017452:Receptors, IgG / Q000037:antagonists & inhibitors / Q000502:physiology; D016897:Respiratory Burst; D012756:Sheep
D000975:Antioxidants; D001555:Benzene Derivatives; D005290:Ferric Compounds; D007074:Immunoglobulin G; D007502:Iron Chelating Agents; D016877:Oxidants; D000644:Quaternary Ammonium Compounds; D017452:Receptors, IgG; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005978:Glutathione; C007164:cumene hydroperoxide; C013531:ferric ammonium citrate