title: Comparative sequence analysis and patterns of covariation in RNA secondary structures.
abstract: A novel method of RNA secondary structure prediction based on a comparison of nucleotide sequences is described. This method correctly predicts nearly all evolutionarily conserved secondary structures of five different RNAs: tRNA, 5S rRNA, bacterial ribonuclease P (RNase P) RNA, eukaryotic small subunit rRNA, and the 3' untranslated region (UTR) of the Drosophila bicoid (bcd) mRNA. Furthermore, covariations occurring in the helices of these conserved RNA structures are analyzed. Two physical parameters are found to be important determinants of the evolution of compensatory mutations: the length of a helix and the distance between base-pairing nucleotides. For the helices of bcd 3' UTR mRNA and RNase P RNA, a positive correlation between the rate of compensatory evolution and helix length is found. The analysis of Drosophila bcd 3' UTR mRNA further revealed that the rate of compensatory evolution decreases with the physical distance between base-pairing residues. This result is in qualitative agreement with Kimura's model of compensatory fitness interactions, which assumes that mutations occurring in RNA helices are individually deleterious but become neutral in appropriate combinations.
authors.affiliation: Department of Biology, University of Rochester, Rochester, New York 14627-0211, USA.
authors.forename: J
authors.initials: J
authors.lastname: Parsch
authors.forename: J M
authors.initials: JM
authors.lastname: Braverman
authors.forename: W
authors.initials: W
authors.lastname: Stephan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004330:Drosophila / Q000235:genetics; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
chemical_list: D012313:RNA