How Strong Is the Coordination Bond between a Histidine Tag and Ni - Nitrilotriacetate? An Experiment of Mechanochemistry on Single Molecules.
Isolation of a His-tagged protein by means of a Ni - nitrilotriacetate-functionalized chromatographic matrix or biosensor was simulated in a single-molecule experiment, and the mechanochemistry-that is, the interplay between mechanical and chemical forces (shown schematically)-was studied with a scanning force microscope.
Conti
M
Dip. di Biochimica, Università di Bologna via Irnerio, 48, 40126 Bologna (Italy).
Falini
G
Samorì
B