title: A multicenter investigation with D-FISH BCR/ABL1 probes.
abstract: Twenty-eight laboratories evaluated a new fluorescence in situ hybridization (FISH) strategy for chronic myeloid leukemia. In a three-part study, bcr/abl1 D-FISH probes were used to study bone marrow specimens. First, laboratories familiarized themselves with the strategy by applying it to known normal and abnormal specimens. Then, collectively the laboratories studied 20 normal and 20 abnormal specimens blindly and measured workload. Finally, each laboratory and two experts studied six serial dilutions with 98-0% abnormal nuclei. Using the reported normal cutoff of < 1% abnormal nuclei, participants reported no false-negative cases and 15 false-positive cases (1-6.6% abnormal nuclei). Results provided by participants for serial dilutions approximated the expected percentages of abnormal nuclei, but those from the experts exhibited greater precision. The clinical sensitivity, precision, nomenclature, workload, recommendations for training, and quality assurance in methods using D-FISH in clinical practice are discussed.
authors.affiliation: Cytogenetics Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: G
authors.initials: G
authors.lastname: Dewald
authors.forename: R
authors.initials: R
authors.lastname: Stallard
authors.forename: A
authors.initials: A
authors.lastname: Alsaadi
authors.forename: S
authors.initials: S
authors.lastname: Arnold
authors.forename: R
authors.initials: R
authors.lastname: Blough
authors.forename: T M
authors.initials: TM
authors.lastname: Ceperich
authors.forename: B
authors.initials: B
authors.lastname: Rafael Elejalde
authors.forename: J
authors.initials: J
authors.lastname: Fink
authors.forename: J V
authors.initials: JV
authors.lastname: Higgins
authors.forename: R R
authors.initials: RR
authors.lastname: Higgins
authors.forename: G A
authors.initials: GA
authors.lastname: Hoeltge
authors.forename: W T
authors.initials: WT
authors.lastname: Hsu
authors.forename: E B
authors.initials: EB
authors.lastname: Johnson
authors.forename: D
authors.initials: D
authors.lastname: Kronberger
authors.forename: D J
authors.initials: DJ
authors.lastname: McCorquodale
authors.forename: L F
authors.initials: LF
authors.lastname: Meisner
authors.forename: M A
authors.initials: MA
authors.lastname: Micale
authors.forename: L
authors.initials: L
authors.lastname: Oseth
authors.forename: J S
authors.initials: JS
authors.lastname: Payne
authors.forename: S
authors.initials: S
authors.lastname: Schwartz
authors.forename: S
authors.initials: S
authors.lastname: Sheldon
authors.forename: A
authors.initials: A
authors.lastname: Sophian
authors.forename: P
authors.initials: P
authors.lastname: Storto
authors.forename: P
authors.initials: P
authors.lastname: Van Tuinen
authors.forename: G D
authors.initials: GD
authors.lastname: Wenger
authors.forename: A
authors.initials: A
authors.lastname: Wiktor
authors.forename: L A
authors.initials: LA
authors.lastname: Willis
authors.forename: J F
authors.initials: JF
authors.lastname: Yung
authors.forename: J
authors.initials: J
authors.lastname: Zenger-Hain
mesh_terms: D001853:Bone Marrow / Q000473:pathology; D019411:Clinical Laboratory Techniques / Q000592:standards*; D005456:Fluorescent Dyes; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods / Q000592:standards; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011786:Quality Control; D012680:Sensitivity and Specificity; D016526:Workload
chemical_list: D005456:Fluorescent Dyes; D016044:Fusion Proteins, bcr-abl