BDNF regulation of androgen receptor expression in axotomized SNB motoneurons of adult male rats.
Brain-derived neurotrophic factor (BDNF) prevents the axotomy-induced loss of androgen receptor-like immunoreactivity (AR-LI) in the spinal nucleus of the bulbocavernosus (SNB) motoneurons of adult male rats. In this report, we investigated the dose-response effect of BDNF on androgen receptor expression in axotomized SNB motoneurons, and examined whether delayed application of BDNF to the cut SNB axons can completely reverse the axotomy-induced loss of androgen receptor expression. We also used autoradiography to test whether axotomy decreases the ability of SNB motoneurons to accumulate androgens. SNB motoneurons were axotomized bilaterally and BDNF or PBS was applied to the proximal ends of the axons. The percentage of SNB motoneurons expressing medium or high AR-LI was the major measure of androgen receptor expression. AR-LI was significantly higher on the BDNF-treated side than on the contralateral side treated with phosphate-buffered saline (PBS) for all three doses of BDNF (1.45, 2.9, and 5.8 mg/ml) and was higher than in rats treated bilaterally with PBS. Moreover, AR-LI at the highest dose of BDNF was not different from that in intact SNB motoneurons. Delayed application of BDNF to the axotomized SNB motoneurons restored the AR-LI to the intact level. The AR-LI decreased by axotomy started to increase significantly 4 days after BDNF application and returned to the intact level by 10 days. Furthermore, axotomy significantly decreased the percentage of SNB motoneurons to accumulate tritiated testosterone or its metabolites. In conclusion, our data demonstrate that BDNF completely prevents and reverses the axotomy-induced loss of AR-LI. Moreover, decrease of AR-LI by axotomy reflects the decrease in the ability of SNB motoneurons to accumulate androgens.
Yang
L Y
LY
Department of Physiological Science and Laboratory of Neuroendocrinology of the Brain Research Institute, Unitcersiry of California, Los Angeles 90095-1527, USA. yangly@ucla.edu
Arnold
A P
AP
D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000187:drug effects; D019771:Axotomy; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009130:Muscle, Smooth / Q000294:innervation*; D009424:Nervous System Physiological Phenomena; D010502:Perineum / Q000294:innervation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
D019208:Brain-Derived Neurotrophic Factor; D011944:Receptors, Androgen; D012965:Sodium Chloride