Internalization kinetics of the gonadotropin-releasing hormone (GnRH) receptor.
This study quantified the agonist-induced endocytotic and recycling events of the mammalian gonadotropin releasing hormone receptor (GnRH-R) and investigated the role of the intracellular carboxyl (C)-terminal tail in regulating agonist-induced receptor internalization kinetics. The rate of internalization for the rat GnRH-R was found to be exceptionally low when compared with G-protein coupled receptors (GPCRs) which possess a cytoplasmic C-terminal tail (thyrotropin-releasing hormone receptor (TRH-R), catfish GnRH-R (cfGnRH-R) and GnRH/TRH-R chimeric receptor). These data provide evidence that the presence of a functional intracellular cytoplasmic C-terminal tail is essential for rapid internalization of the studied GPCRs.
Vrecl
M
M
MRC Reproductive Biology Unit, Edinburgh, United Kingdom.
Heding
A
A
Hanyaloglu
A
A
Taylor
P L
PL
Eidne
K A
KA
D000818:Animals; D002397:Catfishes; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D051381:Rats; D011966:Receptors, LHRH / Q000819:agonists / Q000378:metabolism*
D011966:Receptors, LHRH