Molecular and pharmacological characterization of the canine brainstem alpha-2A adrenergic receptor.
This study characterizes the alpha2-adrenergic receptors present in canine brainstem. Radioligand binding and reverse transcriptase-polymerase chain reaction (RT-PCR) experiments were performed in canine brainstem to identify the receptors present and determine the pharmacological properties of these receptors. The pKi values derived from radioligand competition curves for a number of adrenergic receptor agents at the four alpha2-adrenergic receptor subtypes were compared to the canine brainstem. The pKi values at the canine brainstem alpha2-adrenergic receptor were consistent with the presence of the alpha2A-adrenergic receptor. To determine whether the canine brainstem expressed the message for the alpha2A-adrenergic receptor, RT-PCR was performed with specific primers for the four subtypes of alpha2-adrenergic receptors. In the canine brainstem, only the primers corresponding to a region in the human alpha2A-adrenergic receptor produced a PCR product. No bands were detected in the canine brainstem lanes with the alpha2B-, alpha2C-, or alpha2D-receptor primers. These data suggest that the canine brainstem contains the alpha2A-adrenergic receptor.
Schwartz
D D
DD
Department of Anatomy, Physiology and Pharmacology, Auburn University, College of Veterinary Medicine, AL 36849, USA.
Jones
W G
WG
Hedden
K P
KP
Clark
T P
TP
D058669:Adrenergic alpha-2 Receptor Antagonists; D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000166:cytology / Q000378:metabolism*; D018929:Cell Culture Techniques; D017931:DNA Primers; D004285:Dogs / Q000378:metabolism*; D008168:Lung / Q000166:cytology; D011869:Radioligand Assay / Q000662:veterinary; D051381:Rats; D017207:Rats, Sprague-Dawley; D018341:Receptors, Adrenergic, alpha-2 / Q000145:classification* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D058669:Adrenergic alpha-2 Receptor Antagonists; D017931:DNA Primers; D018341:Receptors, Adrenergic, alpha-2