Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7.
8 h) and high steady-state levels of protein accumulation, while the H2 intrabodies had a half-life of 2 h and less protein at steady state. These results suggest that the choice of sFv as an intrabody depends critically on the intracellular sFv protein having an extended half-life and elevated steady-state level. Thus, extended half-life must be considered together with sFv antibody specificity and affinity when choosing an optimal sFv intrabody for functional studies of cellular proteins.
Zhu
Q
Q
IntraImmune Therapies Inc., P.O. Box 15599, Boston, MA 02215-0011, USA.quanzhu@tiac.net
Zeng
C
C
Huhalov
A
A
Yao
J
J
Turi
T G
TG
Danley
D
D
Hynes
T
T
Cong
Y
Y
DiMattia
D
D
Kennedy
S
S
Daumy
G
G
Schaeffer
E
E
Marasco
W A
WA
Huston
J S
JS
D000818:Animals; D017209:Apoptosis; D053179:Caspase 7; D020169:Caspases / Q000276:immunology*; D002460:Cell Line; D002461:Cell Line, Transformed; D002467:Cell Nucleus; D006224:Cricetinae; D003546:Cysteine Endopeptidases / Q000276:immunology*; D015870:Gene Expression; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D019169:Jurkat Cells; D014162:Transfection
D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; C505520:CASP7 protein, human; D053179:Caspase 7; D020169:Caspases; D003546:Cysteine Endopeptidases