[The use of molecular fingerprinting techniques in environmental hygiene].
The analysis of epidemiological pathways for the transmission of pathogens via the environment is a fundamental task in the field of environmental hygiene. The task demands for a precise differentiation between isolates. This can be done by sophisticated techniques for differentiation which are based on phenotypic or genotypic features. The fingerprinting of DNA is a powerful tool in this context. A survey is given for different kinds of RFLP-analysis, plasmid profiling, and PCR fingerprinting as well regarding their principles, methodical variations and published applications. Notable limitations of the applications are also discussed.
Beyer
W
W
Institut für Umwelt und Tierhyiene sowie Tiermedizin mit Tierklinik der Universität Hohenheim, Stuttgart.
Böhm
R
R
D000820:Animal Diseases / Q000453:epidemiology*; D000818:Animals; D003141:Communicable Diseases / Q000453:epidemiology / Q000662:veterinary*; D016172:DNA Fingerprinting / Q000379:methods*; D004782:Environmental Health; D017720:Molecular Epidemiology / Q000379:methods*; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014730:Veterinary Medicine / Q000379:methods