title: The discriminatory power of ribo-PCR compared to conventional ribotyping for epidemiological purposes.
abstract: Molecular typing techniques have become increasingly important for confirmation of epidemiological relationships and delimitation of nosocomial outbreaks. The discriminatory power of the two DNA-based typing methods, conventional ribotyping and ribo-PCR, was assessed to distinguish between selected strains of Acinetobacter calcoaceticus, Enterobacter cloacae, Serratia marcescens and Pseudomonas aeruginosa. Overall, conventional ribotyping was more discriminatory than ribo-PCR.
authors.affiliation: Molecular Biology Laboratory, Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
authors.forename: P
authors.initials: P
authors.lastname: Severino
authors.forename: A L
authors.initials: AL
authors.lastname: Darini
authors.forename: V D
authors.initials: VD
authors.lastname: Magalhães
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000145:classification / Q000235:genetics; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D016972:Enterobacter cloacae / Q000145:classification / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics; D012706:Serratia marcescens / Q000145:classification / Q000235:genetics
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial