title: Phenylalanine transport in Aspergillus nidulans: demonstration of role of phenylalanine binding proteins.
abstract: Crude shock proteins extracted by two stage osmotic shock were further purified by affinity chromatography to obtain ligand (phenylalanine) specific binding protein (phebip) a component of phenylalanine (phe) transport system from wild type and a phe transport mutant fpaD11 of Aspergillus nidulans. A new eluent 0.1 M Tris-HCl containing 1.5 N NaCl and 0.5 N Na2CO3, pH 8 was used during the investigation. The elution profile of mutant phebip exhibited one simple and two compound peaks instead of three simple ones as exhibited by the wild type phebip. SDS-PAGE profile of mutant phebip showed faster electrophoretic mobility than that of wild type one. It is therefore evident that the mutant phebip has reduced molecular mass (M(r)) due to deletion of a segment that somehow has bearing on the binding capacity of the active site of phebip. The resultant erosion in the binding capacity of the mutant phebip is in turn responsible for its incapability to stimulate transport of ligand across the plasma membrane.
authors.affiliation: Microbial and Molecular Genetics Laboratory, Patna University, India.
authors.forename: S
authors.initials: S
authors.lastname: Sahay
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism*; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D009997:Osmotic Pressure; D010649:Phenylalanine / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D010649:Phenylalanine