[Use of proteases to improve the sensitivity of ABO antigen detection by absorption-elution test in analysis of human excretion traces].
The highest sensitivity of the absorption-elution test for detection of A, B, and H antigens in human excretion traces was observed when the excretion traces were treated with protease C and lidase (hyaluronidase) and standard erythrocytes for analysis of the resultant eluates were treated with protease C. Cross-reactions of sera with antigens of contralateral specificity were ruled out by simple dilution of the sera; the reliability of antigen detection was not lost due to pronounced increase in the sensitivity of modified absorption-elution test.
Sakharov
R S
RS
Fedulova
M V
MV
D000017:ABO Blood-Group System / Q000032:analysis*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001801:Blood Stains; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D006821:Hyaluronoglucosaminidase; D008297:Male; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases; D013094:Spermatozoa
D000017:ABO Blood-Group System; C001543:lidase; D006821:Hyaluronoglucosaminidase; D012697:Serine Endopeptidases; C088701:protease C