title: Effect of inhibitory avoidance training on [3H]-glutamate binding in the hippocampus and parietal cortex of rats.
abstract: Glutamate receptors have been implicated in memory formation. The aim of the present study was to determine the effect of inhibitory avoidance training on specific [3H]-glutamate binding to membranes obtained from the hippocampus or parietal cortex of rats. Adult male Wistar rats were trained (0.5-mA footshock) in a step-down inhibitory avoidance task and were sacrificed 0, 5, 15 or 60 min after training. Hippocampus and parietal cortex were dissected and membranes were prepared and incubated with 350 nM [3H]-glutamate (N = 4-6 per group). Inhibitory avoidance training induced a 29% increase in glutamate binding in hippocampal membranes obtained from rats sacrificed at 5 min (P<0.01), but not at 0, 15, or 60 min after training, and did not affect glutamate binding in membranes obtained from the parietal cortex. These results are consistent with previous evidence for the involvement of glutamatergic synaptic modification in the hippocampus in the early steps of memory formation.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. nadjaschroder@hotmail.com
authors.forename: N
authors.initials: N
authors.lastname: Schröder
authors.forename: F
authors.initials: F
authors.lastname: De-Paris
authors.forename: R
authors.initials: R
authors.lastname: Roesler
authors.forename: J H
authors.initials: JH
authors.lastname: Medina
authors.forename: D O
authors.initials: DO
authors.lastname: Souza
authors.forename: I
authors.initials: I
authors.lastname: Izquierdo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology*; D018698:Glutamic Acid / Q000378:metabolism*; D006624:Hippocampus / Q000502:physiology*; D017774:Long-Term Potentiation / Q000502:physiology; D008297:Male; D008568:Memory / Q000502:physiology*; D010296:Parietal Lobe / Q000502:physiology*; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000378:metabolism*
chemical_list: D017470:Receptors, Glutamate; D018698:Glutamic Acid