Nitric oxide and carbon monoxide modulate oscillations of olfactory interneurons in a terrestrial mollusk.
Spontaneous or odor-induced oscillations in local field potential are a general feature of olfactory processing centers in a large number of vertebrate and invertebrate species. The ubiquity of such oscillations in the olfactory bulb of vertebrates and analogous structures in arthropods and mollusks suggests that oscillations are fundamental to the computations performed during processing of odor stimuli. Diffusible intercellular messengers such as nitric oxide (NO) and carbon monoxide (CO) also are associated with central olfactory structures in a wide array of species. We use the procerebral (PC) lobe of the terrestrial mollusk Limax maximus to demonstrate a role for NO and CO in the oscillatory dynamics of the PC lobe: synthesizing enzymes for NO and CO are associated with the PC lobes of Limax, application of NO to the Limax PC lobe increases the local field potential oscillation frequency, whereas block of NO synthesis slows or stops the oscillation, the bursting cells of the PC lobe that drive the field potential oscillation are driven to higher burst frequency by application of NO, the nonbursting cells of the PC lobe receive trains of inhibitory postsynaptic potentials, presumably from bursting cells, due to application of NO, and application of CO to the PC lobe by photolysis of caged CO results in an increase in oscillation frequency proportional to CO dosage.
Gelperin
A
A
Biological Computation Research Department, Bell Laboratories, Lucent Technologies, Murray Hill, New Jersey 07974, USA. CNSAG@physics.bell-labs.com
Flores
J
J
Raccuia-Behling
F
F
Cooke
I R
IR
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology*; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis*; D017952:Ganglia, Invertebrate / Q000201:enzymology / Q000502:physiology; D006419:Heme Oxygenase (Decyclizing) / Q000032:analysis; D051547:Heme Oxygenase-1; D066298:In Vitro Techniques; D007395:Interneurons / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008974:Mollusca; D009412:Nerve Fibers / Q000201:enzymology / Q000502:physiology; D009569:Nitric Oxide / Q000494:pharmacology*; D009812:Odorants; D009833:Olfactory Pathways / Q000502:physiology; D009991:Oscillometry
D009569:Nitric Oxide; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2; D008058:Dihydrolipoamide Dehydrogenase