Suppressive effects of chlorogenic acid on N-methyl-N-nitrosourea-induced glandular stomach carcinogenesis in male F344 rats.
The modifying effects of chlorogenic acid (CA) on N-methyl-N-nitrosourea (MNU)-induced glandular stomach carcinogenesis were investigated in five groups of male F344 rats. Rats in Groups 1 through 3 were given MNU in drinking water at a concentration of 400 ppm for 12 weeks. Animals of Group 1 were then kept on the basal diet alone, and those of Group 2 or 3 were fed a diet containing 500 or 250 ppm CA for a subsequent 22 weeks. Group 4 was exposed to CA alone through the experimental period (36 weeks), and Group 5 was given the basal diet continuously and treated as a control. At the end of the experiment, the incidence of glandular stomach carcinoma of Group 3 was significantly smaller than that of Group 1 (p < 0.03). The incidence of adenomatous hyperplasia of Group 2 was also significantly lower than that of Group 1 (p < 0.02). In addition, the proliferating cell nuclear antigen (PCNA) labeling index of the epithelial cells from the non-neoplastic mucosa in rats of Group 2 or 3 was significantly smaller than that of Group 1 (p < 0.0001). These results suggest that CA has a chemopreventive effect on MNU-induced rat glandular stomach carcinogenesis by exposure during the post-initiation phase, and CA may be a promising agent for prevention of human stomach cancer.
Shimizu
M
M
Department of Pathology, Gifu University School of Medicine, Japan.
Yoshimi
N
N
Yamada
Y
Y
Matsunaga
K
K
Kawabata
K
K
Hara
A
A
Moriwaki
H
H
Mori
H
H
D000818:Animals; D002273:Carcinogens; D002726:Chlorogenic Acid / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D013274:Stomach Neoplasms / Q000139:chemically induced* / Q000517:prevention & control
D002273:Carcinogens; D018809:Proliferating Cell Nuclear Antigen; D002726:Chlorogenic Acid; D008770:Methylnitrosourea