A modified Boyden chamber assay for tumor cell transendothelial migration in vitro.
We have developed a modified Boyden chamber system for assessing tumor cell transmigration across endothelial monolayer in vitro. This radiometric-based method allows us to take into account the cells which have traversed but detached from the filter, a significant fraction not included in the existing method of visual counting. The method was applied to examine six malignant and one non-malignant cell lines. The variable invasive potentials of these cells were found to correlate with their ability to disrupt the endothelial cell monolayers.
Li
Y H
YH
George W. Woodruff School of Mechanical Engineering and Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta 30332-0405, USA.
Zhu
C
C
D001681:Biological Assay / Q000295:instrumentation*; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000473:pathology; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured