title: Lithium chloride inhibits thrombin-induced intracellular calcium mobilization in C6 rat glioma cells.
abstract: In this study, the authors have demonstrated the effect of lithium, a typical mood stabilizer, on thrombin-evoked Ca2+ mobilization in C6 cells to elucidate the action mechanisms of the drug. Thrombin-induced Ca2 mobilization was reduced 24 hr after 1 or 10 mM lithium chloride (LiCl) pretreatment. The Ca2+ rise was reduced in a time-dependent manner, and the significant inhibition was observed 9 hr pretreatment with 10 mM LiCl. On the other hand, pretreatment of the cells with 10 mM LiCl for 24 hr did not alter the amount of Galphaq/11 significantly. Pretreatment with 10 mM LiCl for 24 hr failed to reduce the 5-HT-induced Ca2+ mobilization or to affect the desensitization of the 5-HT signal. Finally, thrombin-elicited Ca2+ rise was markedly inhibited in the presence of 0.05 U/ml plasmin, however, the Ca2+ rise was not further attenuated in the presence of plasmin in C6 cells pretreated with LiCl for 24 hr. These results indicate that pretreatment with LiCl attenuated thrombin-evoked intracellular Ca2+ mobilization in plasmin sensitive manner in C6 rat glioma cells. Thus, it is important to investigate the effect of lithium on thrombin-induced cellular responses to clarify the action mechanism of lithium in relation to some abnormality in thrombin-evoked Ca2+ rise observed in bipolar disorders.
authors.affiliation: Department of Psychiatry and Neurosciences, Hiroshima University School of Medicine, Japan. kagaya@ue.ipc.hiroshima-u.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Kagaya
authors.forename: A
authors.initials: A
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Tawara
authors.forename: M
authors.initials: M
authors.lastname: Inagaki
authors.forename: H
authors.initials: H
authors.lastname: Jitsuiki
authors.forename: T
authors.initials: T
authors.lastname: Kozuru
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
authors.forename: H
authors.initials: H
authors.lastname: Katagiri
authors.forename: Y
authors.initials: Y
authors.lastname: Uchitomi
authors.forename: J
authors.initials: J
authors.lastname: Horiguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakata
authors.forename: S
authors.initials: S
authors.lastname: Yamawaki
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005341:Fibrinolysin / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism*; D018021:Lithium Chloride / Q000494:pharmacology*; D051381:Rats; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology; D013917:Thrombin / Q000037:antagonists & inhibitors*; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D005343:Fibrinolytic Agents; D044402:Receptor, Serotonin, 5-HT2A; D011985:Receptors, Serotonin; D012701:Serotonin; D013917:Thrombin; D005341:Fibrinolysin; D018021:Lithium Chloride; D002118:Calcium