title: Genomic fingerprinting of pigeon Streptococcus gallolyticus strains of different virulence by randomly amplified polymorphic DNA (RAPD) analysis.
abstract: Randomly amplified polymorphic DNA (RAPD) analysis was performed on 95 pigeon S. gallolyticus strains of different virulence and belonging to different biotypes and different culture supernatant phenotypes as determined by SDS-PAGE. Four distinct RAPD patterns, designated A, B, C and D, were distinguished using primer OPM6 (5'CTGGGCAACT). All 76 strains generating RAPD pattern A or B were designated highly virulent on the basis of their SDS-PAGE pattern. Five of seven strains generating RAPD pattern C and 11 of 12 strains generating RAPD pattern D belonged to the moderately virulent and low virulent culture supernatant phenotype groups, respectively. Only one RAPD group C strain belonged to a highly virulent culture supernatant phenotype group. There was a correlation between biotype and RAPD patterns. These findings indicate that there is a high correlation between RAPD pattern and virulence for pigeons. Therefore, RAPD typing seems a rapid, reliable method to distinguish pigeon S. gallolyticus strains of high, moderate and low virulence.
authors.affiliation: Department of Pathology, Bacteriology and Poultry Diseases, University of Gent, Merelbeke, Belgium. margo.baele@rug.ac.be
authors.forename: M
authors.initials: M
authors.lastname: Baele
authors.forename: M
authors.initials: M
authors.lastname: Vanrobaeys
authors.forename: M
authors.initials: M
authors.lastname: Vaneechoutte
authors.forename: P
authors.initials: P
authors.lastname: De Herdt
authors.forename: L A
authors.initials: LA
authors.lastname: Devriese
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000382:microbiology*; D010856:Columbidae / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary*; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
chemical_list: D004269:DNA, Bacterial