Induction of micronuclei by CsCl in vivo and in vitro.
In the present study, we report the results of an investigation of the potential of nonradioactive CsCl for the induction of micronuclei in polychromatic erythrocytes of mouse bone marrow and in human lymphocytes cultured and blocked with cytochalasin-B. No significant increase in micronucleus frequency was observed in the polychromatic erythrocytes of mice which received 500 mg/kg of CsCl. In vitro experiments with human lymphocytes cultured in medium containing 250 and 500 micrograms/ml CsCl also showed no increase in micronucleus frequency compared to untreated controls. These same experiments, however, demonstrated a reduction in mitotic activity with increasing CsCl concentration in the culture medium. This report is the first to describe studies on the possible induction of micronuclei in vitro and in vivo by nonradioactive CsCl.
Santos-Mello
R
R
Departamento de Ciências Biológicas, Universidade Luterana do Brasil, Canoas, Brazil. mello@ulbra.br
Schmidt
T
T
Neuhauss
E
E
Almeida
A
A
D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects*; D002586:Cesium / Q000633:toxicity*; D002712:Chlorides / Q000633:toxicity*; D003520:Cyclophosphamide / Q000633:toxicity; D003571:Cytochalasin B / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity
D002712:Chlorides; D009153:Mutagens; D002586:Cesium; D003571:Cytochalasin B; D003520:Cyclophosphamide; C028019:cesium chloride