title: [Interactions of the Pseudomonas aeruginosa outer membrane proteins with plasma fibronectins. Bacterial adhesin investigation].
abstract: Pseudomonas aeruginosa adherence is a complex phenomenon largely mediated by pili involving specific receptor-ligand interactions. Anti-fibronectin antibodies as well as plasmatic fibronectin are able to inhibit P. aeruginosa adherence onto A549 cells showing that matricial fibronectin is an actual receptor for this bacterium. Experiments performed in vitro with human plasmatic fibronectin used as receptor and outer membrane proteins of P. aeruginosa as ligands show the presence of four fibronectin-binding proteins. These proteins with molecular mass of 70 +/- 2, 60 +/- 2, 48 +/- 2 and 36 +/- 1 kDa should be adhesins of P. aeruginosa on epithelial cell matrix in a non-pilus mediated adherence.
authors.affiliation: Université de Cergy-Pontoise, UFR sciences et techniques, Cergy-Pontoise, France.
authors.forename: J
authors.initials: J
authors.lastname: Rebière-Huët
authors.forename: P
authors.initials: P
authors.lastname: Di Martino
authors.forename: O
authors.initials: O
authors.lastname: Gallet
authors.forename: C
authors.initials: C
authors.lastname: Hulen
mesh_terms: D018829:Adhesins, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D001422:Bacterial Adhesion / Q000502:physiology*; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D005353:Fibronectins / Q000097:blood*; D010861:Fimbriae, Bacterial / Q000502:physiology; D006801:Humans; D008168:Lung / Q000166:cytology; D008970:Molecular Weight; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000502:physiology*
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D005353:Fibronectins