Metabolites of the biocide o-phenylphenol generate oxidative DNA lesions in V 79 cells.
Incubation of the o-phenylphenol (OPP) metabolites, o-phenylhydroquinone (PHQ) and o-phenylbenzoquinone (PBQ) with V 79 Chinese hamster cells led to a significant enhancement of the amount of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in nuclear DNA. With OPP no distinct induction of this lesion could be observed. In addition, PHQ and PBQ were able to generate DNA single-strand breaks (DNA SSB), while OPP failed to induce this lesion. All incubations were performed for 1 h without exogenous metabolic activations and the lowest effective concentration tested was 20 microM. It is concluded that these metabolites may contribute to the carcinogenicity of OPP and sodium o-phenylphenolate (SOPP) observed in rats, by generating reactive oxygen species (ROS) through their redox cycling properties.
Henschke
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P
Division for Plant Protection Products and Pesticides, Federal Institute for Health Protection of Consumers and Veterinary Medicine, 14191 Berlin, Germany.
Almstadt
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Lüttgert
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Appel
K E
KE
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D016227:Benzoquinones / Q000378:metabolism / Q000633:toxicity*; D001713:Biphenyl Compounds / Q000378:metabolism / Q000633:toxicity*; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D018736:DNA Adducts / Q000096:biosynthesis; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005659:Fungicides, Industrial / Q000378:metabolism* / Q000633:toxicity*; D006873:Hydroquinones / Q000378:metabolism / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism
D016227:Benzoquinones; D001713:Biphenyl Compounds; D002273:Carcinogens; D018736:DNA Adducts; D004277:DNA, Single-Stranded; D005659:Fungicides, Industrial; D006873:Hydroquinones; D017382:Reactive Oxygen Species; C029322:phenylbenzoquinone; D000080242:8-Hydroxy-2'-Deoxyguanosine; C004369:2-phenylphenol; D003849:Deoxyguanosine; C066330:phenylhydroquinone