Anti-calreticulin segregates anti-Ro sera in systemic lupus erythematosus: anti-calreticulin is present in sera with anti-Ro alone but not in anti-Ro sera with anti-La or anti-ribonucleoprotein.
OBJECTIVE
To examine a well characterized group of patients with systemic lupus erythematosus (SLE) for anti-calreticulin.


METHODS
The sera of 77 patients with SLE were studied by immunodiffusion, solid phase immunoassay, and immunoblot for antibodies against ribonucleoprotein (RNP) autoantigens and calreticulin.


RESULTS
Thirty-five had anti-calreticulin and 40 had anti-60 kDa Ro. There was no association of anti-60 kDa Ro and anti-calreticulin. However, among anti-60 kDa Ro positive sera that also contained either anti-La or anti-RNP, none of 18 had anti-calreticulin. All the remaining sera with anti-60 kDa Ro had anti-calreticulin and anti-52 kDa Ro.


CONCLUSIONS
Anti-60 kDa Ro patients with SLE can be divided into those with anti-60 kDa Ro and either anti-La or anti-RNP or those with anti-60 kDa Ro, anti-52 kDa Ro, and anti-calreticulin.
Scofield
R H
RH
Department of Medicine, University of Oklahoma Health Sciences Center, the Veterans Affairs Medical Center, and the W.K. Warren Medical Research Institute, Oklahoma City, USA. hal-scofield@omrf.ouhsc.edu
Racila
D M
DM
Gordon
T P
TP
Kurien
B T
BT
Sontheimer
R D
RD
D001324:Autoantigens / Q000097:blood* / Q000276:immunology*; D002135:Calcium-Binding Proteins / Q000276:immunology*; D037282:Calreticulin; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000276:immunology*; D014157:Transcription Factors / Q000276:immunology*; D000097585:SS-B Antigen; D000099207:SS-A Antigen
D001324:Autoantigens; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C116006:RO60 protein, human