title: Study on facial motoneuronal death after proximal or distal facial nerve transection.
abstract: BACKGROUND
  Among the cranial nerves, the facial nerve is most liable to be damaged. Pathologic changes in the facial motor nucleus (FMN) after nerve injury are not well recognized, and the optimal time for facial nerve reconstruction after axotomy is controversial. In this study, to clarify the pathologic change in the FMN after axotomy and to determine the best time for surgery, facial motoneuronal death was investigated after facial nerve injury.
  
  
  METHODS
  Sixty Wistar rats were divided into proximal and distal groups. In the proximal group, the right facial nerve was transected at the porus of internal acoustic meatus. In the distal group, the nerve was cut at the stylomastoid foramen. The animals were sacrificed from day 3 to day 60 after surgery, and the brainstem was fixed with 4% paraformaldehyde. The FMN were then examined in serial sections stained with Cresyl Violet, and facial motoneurons were counted under a light microscope.
  
  
  RESULTS
  The death rate of facial motoneurons in the animals that underwent proximal axotomy was found to be higher and cell death occurred earlier than in the distal axotomy animals at every time point. Moreover, neuron death increased with time and peaked at 15 days after surgery.
  
  
  CONCLUSIONS
  The results indicate that the injury site was correlated with facial motoneuronal death, and suggest that reconstructive surgery should be performed as early as possible.
authors.affiliation: Department of Otolaryngology, Eye, Ear, Nose and Throat Hospital, Shanghai Medical University, China.
authors.forename: C F
authors.initials: CF
authors.lastname: Dai
authors.forename: N
authors.initials: N
authors.lastname: Kanoh
authors.forename: K Y
authors.initials: KY
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D016923:Cell Death / Q000502:physiology; D005154:Facial Nerve / Q000473:pathology* / Q000601:surgery*; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D009410:Nerve Degeneration / Q000473:pathology; D019635:Neurosurgical Procedures / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar