title: Angiotensin I-converting enzyme antisense prevents altered renal vascular reactivity, but not high blood pressure, in spontaneously hypertensive rats.
abstract: The renin-angiotensin system plays a critical role in the control of blood pressure, and its hyperactivity is associated with the development of human primary hypertension. Because low-dose angiotensin I-converting enzyme (ACE) inhibitors cause small reductions in blood pressure that are associated with the complete reversal of altered vascular pathophysiology, our objective in this study was to determine whether ACE antisense (ACE-AS) gene delivery prevents alterations in renal vascular physiology in the parents and F(1) offspring of AS-treated spontaneously hypertensive rats (SHR). A single bolus intracardiac injection of ACE-AS (2x10(8) colony-forming units) in SHR neonates caused a modest (18+/-3 mm Hg, n=7 to 9) lowering of blood pressure, which was maintained in the F(1) generation offspring (n=7 to 9). Alterations in renal vascular reactivity, electrophysiology, and [Ca(2+)](i) homeostasis are underlying mechanisms associated with the development and establishment of hypertension. Renal resistance arterioles from truncated ACE sense-treated SHR showed a significantly enhanced contractile response to KCl and phenylephrine (n=24 rings from 6 animals, P<0.01) and significantly attenuated acetylcholine-induced relaxations (n=24 rings from 6 animals, P<0.01) compared with arterioles from ACE-AS-treated SHR. In addition, compared with cells dissociated from arterioles of ACE-AS-treated SHR, cells from truncated ACE sense-treated animal vessels had a resting membrane potential that was 22+/-4 mV more depolarized (n=38, P<0.01), an enhanced L-type Ca(2+) current density (2.2+/-0.3 versus 1.2+/-0.2 pA/pF, n=23, P<0.01), a decreased Kv current density (16.2+/-1.3 versus 5.4+/-2.2 pA/pF, n=34, P<0.01), and increased Ang II-dependent changes in [Ca(2+)](i) (n=142, P<0.01). Similar effects of ACE-AS treatment were observed in the F(1) offspring. These results demonstrate that ACE-AS permanently prevents alterations in renal vascular pathophysiology in spite of the modest effect that ACE-AS had on high blood pressure in SHR.
authors.affiliation: Department of Physiology, University of Florida College of Medicine, Gainesville, FL 32610, USA. Gelband@phys.med.ufl.edu
authors.forename: C H
authors.initials: CH
authors.lastname: Gelband
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: M L
authors.initials: ML
authors.lastname: Gardon
authors.forename: K
authors.initials: K
authors.lastname: Keene
authors.forename: D S
authors.initials: DS
authors.lastname: Goldberg
authors.forename: P Y
authors.initials: PY
authors.lastname: Reaves
authors.forename: M J
authors.initials: MJ
authors.lastname: Katovich
authors.forename: M K
authors.initials: MK
authors.lastname: Raizada
mesh_terms: D000818:Animals; D001160:Arterioles / Q000737:chemistry / Q000502:physiology; D001794:Blood Pressure; D002118:Calcium / Q000493:pharmacokinetics; D015220:Calcium Channels / Q000502:physiology; D002460:Cell Line; D004594:Electrophysiology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015316:Genetic Therapy; D006706:Homeostasis / Q000502:physiology; D006977:Hypertension, Renal / Q000235:genetics* / Q000628:therapy*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000201:enzymology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D015221:Potassium Channels / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012079:Renal Circulation; D012190:Retroviridae / Q000235:genetics
chemical_list: D015220:Calcium Channels; D016376:Oligonucleotides, Antisense; D015221:Potassium Channels; D011189:Potassium Chloride; D007703:Peptidyl-Dipeptidase A; D002118:Calcium