The feasibility of PCR-based diagnosis of Prader-Willi and Angelman syndromes using restriction analysis after bisulfite modification of genomic DNA.
We have developed a novel PCR-based method for studying DNA methylation in the proximal region of 15q, using restriction analysis after bisulfite treatment of genomic DNA. This protocol can be used for the diagnosis of Prader-Willi and Angelman syndromes. Unlike the recently reported methylation-specific PCR protocol, our method avoids the use of multiplex amplification, thus overcoming the need to adjust relative primer amounts and the risk of obtaining false-negative results.
Velinov
M
M
NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York, 10314, USA.
Gu
H
H
Genovese
M
M
Duncan
C
C
Brown
W T
WT
Jenkins
E
E
D017204:Angelman Syndrome / Q000175:diagnosis* / Q000235:genetics; D001324:Autoantigens / Q000235:genetics; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D017931:DNA Primers / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005188:False Negative Reactions; D005820:Genetic Testing / Q000379:methods; D016678:Genome; D018392:Genomic Imprinting / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011218:Prader-Willi Syndrome / Q000175:diagnosis* / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear; D013447:Sulfites / Q000378:metabolism*; D055517:snRNP Core Proteins
D001324:Autoantigens; D017931:DNA Primers; D017411:Ribonucleoproteins, Small Nuclear; D013447:Sulfites; D055517:snRNP Core Proteins; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases