Isolation and initial characterization of 13(2)-hydroxychlorophyll a induced by cyclohexanedione derivatives in tobacco cell suspension cultures.
This paper reports that a new photobleaching compound, 2-(2-chloro-5-propoxycarbonylphenyl)aminomethylidene-5-5-dim ethyl- cyclohexane-1,3-dione (RWH-21), stimulates accumulation of 13(2)-hydroxychlorophyll a in cultured tobacco cells. This was shown based on isolation of 13(2)-hydroxychlorophyll a from pigment extracts of cultured tobacco cells by diode-array HPLC and subsequent fast atom bombardment mass spectrometry analysis. 13(2)-Hydroxychlorophyll a rapidly accumulates in tobacco cells both in the light and dark in the presence of RWH-21 (50 microM). Analysis of 13(2)-hydroxychlorophyll a formation in tobacco cells indicates that 13(2)-hydroxychlorophyll a is rapidly accumulated within 20 h incubation time both in the dark and light. Although the amount of 13(2)-hydroxychlorophyll a is continuously increased in the dark, the amount of 13(2)-hydroxychlorophyll a decreased remarkably in the light after 20 h incubation. Analysis of 13(2)-hydroxychlorophyll a formation and lipid peroxidation by determination malondialdehyde in tobacco cells suggests that RWH-21-induced 13(2)-hydroxychlorophyll a has the potential to cause a photodynamic action in cultured tobacco cells.
Wang
J M
JM
Department of Biotechnology, Akita Prefectural University, Japan. jmwang@akita-pu.ac.jp
Asami
T
T
Murofushi
N
N
Yoshida
S
S
D002478:Cells, Cultured; D002734:Chlorophyll / Q000097:blood / Q000737:chemistry* / Q000302:isolation & purification; D000077194:Chlorophyll A; D003512:Cyclohexanones / Q000494:pharmacology*; D010947:Plants, Toxic; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D003512:Cyclohexanones; D002734:Chlorophyll; C018031:1,2-cyclohexanedione; D000077194:Chlorophyll A