title: Regulation of phosphatidylcholine biosynthesis by mGluR1alpha expressed in human embryonic kidney 293 cells--A 31P-NMR study.
abstract: A recent report has demonstrated that inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release plays a crucial role in neurite growth. Here, using 31P-NMR, we examine whether activation of the metabotropic glutamate receptor 1 (mGluR1), which induces the production of IP3, could modulate phospholipid metabolism in human embryonic kidney 293 cells. mGluR1alpha- but not ionotropic glutamate receptor 1-expressing cells stimulated with glutamate exhibited a drastic reduction in the phosphorylcholine level, with corresponding increases in the level of phosphatidylcholine, a major phospholipid component. Quantitative analysis of cell growth revealed that mGluR1alpha-expressing cells cultured with 100microM glutamate were statistically significantly longer than the nontransfected cells. The effect was no longer observed following coincubation with a metabotropic glutamate receptor antagonist, (RS)-alpha-methyl-4-carboxyphenylglycine. These results suggest that mGluR1alpha activation triggers phosphatidylcholine biosynthesis and may contribute to neurite extension.
authors.affiliation: Laboratory for Memory & Learning, RIKEN Brain Science Institute, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: K
authors.initials: K
authors.lastname: Yoshioka
authors.forename: K
authors.initials: K
authors.lastname: Yamada
mesh_terms: D002460:Cell Line; D048429:Cell Size; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007668:Kidney; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018408:Patch-Clamp Techniques; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010758:Phosphorus; D018091:Receptors, AMPA / Q000235:genetics / Q000502:physiology*; D018094:Receptors, Metabotropic Glutamate / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D015544:Inositol 1,4,5-Trisphosphate; D010713:Phosphatidylcholines; D010758:Phosphorus; D018091:Receptors, AMPA; D018094:Receptors, Metabotropic Glutamate; D011994:Recombinant Proteins; C104077:metabotropic glutamate receptor type 1