title: A colicin-tolerant Escherichia coli mutant that confers hfl phenotype carries two mutations in the region coding for the C-terminal domain of FtsH (HflB).
abstract: An Escherichia coli mutant, ER437, which was originally isolated for colicin tolerance, was found to carry two amino acid changes in the C-terminal portion of FtsH (HflB). These mutations were demonstrated to reduce the ability of FtsH to degrade the phage lambda CII protein in vivo and in vitro, providing a rationalization for the mutant Hfl phenotype.
authors.affiliation: Department of Molecular Genetics and Biotechnology, The Hebrew University-Hadassah Medical School, P.O. Box 12272, 91120, Jerusalem, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Teff
authors.forename: S
authors.initials: S
authors.lastname: Koby
authors.forename: Y
authors.initials: Y
authors.lastname: Shotland
authors.forename: T
authors.initials: T
authors.lastname: Ogura
authors.forename: A B
authors.initials: AB
authors.lastname: Oppenheim
mesh_terms: D049069:ATP-Dependent Proteases; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D010582:Bacteriophage lambda / Q000502:physiology; D003001:Cloning, Molecular; D003087:Colicins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000821:virology; D029968:Escherichia coli Proteins; D015967:Gene Expression Regulation, Viral; D008242:Lysogeny / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D011993:Recombinant Fusion Proteins; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000378:metabolism; D014161:Transduction, Genetic; D014764:Viral Proteins
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C039000:cII protein, bacteriophage lambda; D049069:ATP-Dependent Proteases; C513383:FtsH protein, E coli