title: Cloning and sequencing of mouse glutaredoxin (grx) cDNA.
abstract: Glutaredoxins are small proteins (12 kDa) with a conserved active sequence Cys-Pro-Tyr(-Phe)-Cys that catalyse GSH-disulfide oxidoreduction reactions in the presence of NADPH and glutathione reductase. Many mammalian glutaredoxins have been characterized and human and pig cDNA sequence determined. However, no mouse glutaredoxin cDNA or protein sequence has yet been reported. We have cloned a cDNA from a mouse liver library that encodes the putative mouse glutaredoxin homologue. The deduced polypeptide sequence encodes a 107 amino acid protein displaying a high degree of homology with other members of the glutaredoxin family.
authors.affiliation: Dept. of Biosciences at NOVUM, Center for Biotechnology, Karolinska Institutet, Huddinge, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Miranda-Vizuete
authors.forename: J R
authors.initials: JR
authors.lastname: Pedrajas
authors.forename: A E
authors.initials: AE
authors.lastname: Damdimopoulos
authors.forename: G
authors.initials: G
authors.lastname: Spyrou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D054477:Glutaredoxins; D006801:Humans; D008322:Mammals / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; C516005:GLRX protein, human; D054477:Glutaredoxins; D011506:Proteins; D010088:Oxidoreductases