Characterization of a 29-kDa beta-1,3-glucanase from Trichoderma harzianum.
A beta-1,3-glucanase, from culture filtrates of Trichoderma harzianum, was purified in sequential steps by gel filtration, hydrophobic interaction and ion exchange chromatography. A typical procedure provided 69-fold purification with 0.32% yield. The molecular mass of the protein was found to be approximately 29 kDa, as estimated by SDS-PAGE on a 10% slab gel. The K(M) and V(max) values for beta-1,3-glucanase, using laminarin as substrate, were 1. 72 mg ml(-1) and 3.10 U ml(-1), respectively. The pH optimum for the enzyme was pH 4.4 and maximum activity was obtained at 50 degrees C. The enzyme was strongly inhibited by HgCl(2) and SDS. These results suggest that each beta-1,3-glucanase produced by T. harzianum is different and is probably encoded by different genes.
Noronha
E F
EF
Departamento de Biologia Celular, Universidade de Brasilia, Brasilia, Brazil.
Ulhoa
C J
CJ
D002686:Chitin / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D043326:Glucan 1,3-beta-Glucosidase; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development; D001617:beta-Glucosidase / Q000302:isolation & purification* / Q000378:metabolism*
D003470:Culture Media; D002686:Chitin; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase