title: A microbial biosensor based on Yarrowia lipolytica for the off-line determination of middle-chain alkanes.
abstract: A microbial biosensor based on immobilised psychrotrophic yeast Yarrowia lipolytica integrated to FIA for the determination of middle chain alkanes was developed. The system responded very well to middle chain alkanes even at low operational temperatures down to +5 degrees C. The maximum sensitivity was obtained at 15 degrees C. A linear relationship was observed between the sensor response and dodecane concentration up to 100 microM.
authors.affiliation: Department of Biotechnology, Lund University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Alkasrawi
authors.forename: R
authors.initials: R
authors.lastname: Nandakumar
authors.forename: R
authors.initials: R
authors.lastname: Margesin
authors.forename: F
authors.initials: F
authors.lastname: Schinner
authors.forename: B
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D000473:Alkanes / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism; D005069:Evaluation Studies as Topic; D010578:Petroleum / Q000032:analysis / Q000378:metabolism; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D000473:Alkanes; D004785:Environmental Pollutants; D010578:Petroleum