ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium.
The establishment of reliable serological methods for cysticercosis in pigs is important for the surveillance, control and prevention of taeniosis/cysticercosis in humans as well as in pigs to prevent economic loss. Both ELISA and immunoblot using glycoproteins (GPs) purified by a single step of preparative iso-electric focusing, which are highly useful for human cysticercosis, have been applied for a serological study in pigs naturally infected with Taenia solium. All sera from pigs showed similar responses to those in human cysticercosis. Therefore, it is expected that both ELISA and immunoblots using GPs would be useful in differentiating infected pigs from uninfected ones.
Ito
A
A
Department of Parasitology, Asahikawa Medical College, Japan. akiraito@asahikawa-med.ac.jp
Plancarte
A
A
Nakao
M
M
Nakaya
K
K
Ikejima
T
T
Piao
Z X
ZX
Kanazawa
T
T
Margono
S S
SS
D000818:Animals; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D015151:Immunoblotting / Q000379:methods / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D006023:Glycoproteins