title: Requirement of CD9 on the egg plasma membrane for fertilization.
abstract: CD9 is an integral membrane protein associated with integrins and other membrane proteins. Mice lacking CD9 were produced by homologous recombination. Both male and female CD9-/- mice were born healthy and grew normally. However, the litter size from CD9-/- females was less than 2% of that of the wild type. In vitro fertilization experiments indicated that the cause of this infertility was due to the failure of sperm-egg fusion. When sperm were injected into oocytes with assisted microfertilization techniques, however, the fertilized eggs developed to term. These results indicate that CD9 has a crucial role in sperm-egg fusion.
authors.affiliation: Institute of Life Science, Kurume University, Kurume, Fukuoka 839-0861, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyado
authors.forename: G
authors.initials: G
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Hasuwa
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: F
authors.initials: F
authors.lastname: Ryu
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kosai
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: A
authors.initials: A
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Okabe
authors.forename: E
authors.initials: E
authors.lastname: Mekada
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003433:Crosses, Genetic; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization / Q000502:physiology; D005307:Fertilization in Vitro; D018390:Gene Targeting; D007247:Infertility, Female / Q000503:physiopathology*; D039121:Integrin alpha6beta1; D016023:Integrins / Q000502:physiology; D008098:Litter Size; D008297:Male; D008562:Membrane Glycoproteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000276:immunology / Q000502:physiology*; D010060:Ovulation; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D060245:Tetraspanin 29
chemical_list: D015703:Antigens, CD; C554870:Cd9 protein, mouse; D039121:Integrin alpha6beta1; D016023:Integrins; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29