title: Neuronal pyruvate carboxylation supports formation of transmitter glutamate.
abstract: Release of transmitter glutamate implies a drain of alpha-ketoglutarate from neurons, because glutamate, which is formed from alpha-ketoglutarate, is taken up by astrocytes. It is generally believed that this drain is compensated by uptake of glutamine from astrocytes, because neurons are considered incapable of de novo synthesis of tricarboxylic acid cycle intermediates, which requires pyruvate carboxylation. Here we show that cultured cerebellar granule neurons form releasable [(14)C]glutamate from H(14)CO(3)(-) and [1-(14)C]pyruvate via pyruvate carboxylation, probably mediated by malic enzyme. The activity of pyruvate carboxylation was calculated to be approximately one-third of the pyruvate dehydrogenase activity in neurons. Furthermore, intrastriatal injection of NaH(14)CO(3) or [1-(14)C]pyruvate labeled glutamate better than glutamine, showing that pyruvate carboxylation occurs in neurons in vivo. This means that neurons themselves to a large extent may support their release of glutamate, and thus entails a revision of the current view of glial-neuronal interactions and the importance of the glutamine cycle.
authors.affiliation: Norwegian Defense Research Institute, Division for Environmental Toxicology, N-2027 Kjeller, Norway. bjornar.hassel.ffi.no
authors.forename: B
authors.initials: B
authors.lastname: Hassel
authors.forename: A
authors.initials: A
authors.lastname: Brâthe
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000096:biosynthesis; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002952:Citric Acid Cycle; D005649:Fumarate Hydratase / Q000378:metabolism; D018698:Glutamic Acid / Q000096:biosynthesis*; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology; D009574:Nitro Compounds; D011422:Propionates / Q000494:pharmacology; D011773:Pyruvates / Q000378:metabolism*; D051381:Rats
chemical_list: D001639:Bicarbonates; D002250:Carbon Radioisotopes; D009498:Neurotoxins; D009574:Nitro Compounds; D011422:Propionates; D011773:Pyruvates; D001224:Aspartic Acid; D018698:Glutamic Acid; D008291:Malate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex; D002950:Citrate (si)-Synthase; D005649:Fumarate Hydratase; C015392:3-nitropropionic acid