title: Physiological role of endothelin-1 in nonworking muscles during exercise in healthy subjects.
abstract: Endothelin-1 (ET-1) is a potent vasoconstrictor peptide produced by vascular endothelial cells. However, the role of ET-1 in exercise-induced physiological responses is still to be investigated. The purpose of the present study was to investigate in healthy volunteers whether the ET-1 plasma concentration in nonworking muscles is changed by exercise and to investigate the physiological role of ET-1 during exercise. Bicycle ergometer cardiopulmonary exercise tests were performed in 36 healthy men (mean age, 22.5 years). Blood samples for measuring ET-1 were drawn from the cubital vein during rest and immediately after the exercise test. The ET-1 change ratio was calculated as ET-1 immediately following exercise/ET-1 during the resting state. Cardiac output (CO) was measured during the exercise test by the impedance method. Arterial venous oxygen difference (AVO2D) when CO reached 10L/min or 15L/min was calculated as AVO2D = VO2/CO. Results were as follows: (1) the ET-1 change ratio correlated inversely with exercise time at the anaerobic threshold (r = -0.37, p = 0.03) and peak exercise time (r = -0.35, p = 0.04); (2) the ET-1 change ratio tended toward an inverse correlation with deltaVO2/deltawork rate (r = -0.29, p = 0.09); (3) the ET-1 change ratio correlated positively with AVO2D when CO reached 10L/min (r = 0.42, p = 0.02) and tended toward a positive correlation with AVO2D when CO reached 15 L/min (r = 0.32, p = 0.08). These results indicate that an increase in ET-1 in nonworking muscles may participate in the exercise-induced redistribution of blood flow and in increasing the blood flow to working muscles.
authors.affiliation: Department of Cardiology, St Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tanabe
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: N
authors.initials: N
authors.lastname: Osada
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: Y
authors.initials: Y
authors.lastname: Akashi
authors.forename: A
authors.initials: A
authors.lastname: Seki
authors.forename: H
authors.initials: H
authors.lastname: Samejima
authors.forename: M
authors.initials: M
authors.lastname: Oya
authors.forename: T
authors.initials: T
authors.lastname: Murabayashi
authors.forename: K
authors.initials: K
authors.lastname: Omiya
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: F
authors.initials: F
authors.lastname: Miyake
authors.forename: M
authors.initials: M
authors.lastname: Murayama
mesh_terms: D000328:Adult; D001794:Blood Pressure; D002302:Cardiac Output; D019332:Endothelin-1 / Q000097:blood*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D006339:Heart Rate; D006439:Hemodynamics / Q000502:physiology*; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D010100:Oxygen / Q000097:blood; D005082:Physical Exertion / Q000502:physiology; D012016:Reference Values; D012044:Regression Analysis; D012146:Rest
chemical_list: D019332:Endothelin-1; D010100:Oxygen