Effect of chromogranin A (pancreastatin) fragment on invasion of prostate cancer cells.
We investigated the effect of chromogranin A (pancreastatin) fragment on the invasion of PC-3, DU-145 and LNCaP prostate cancer cells through a reconstituted basement membrane (Matrigel) using a Transwell cell culture chamber assay. Chromogranin A fragment increased the invasive capacity of both PC-3 and DU- 145 cells, whereas it had no significant effect of LNCaP cells. Chromogranin A fragment also increased the haptotactic migration of both PC-3 and DU-145 cells to fibronectin. Furthermore chromogranin A fragment increased the fibrinolytic activities of urokinase-type plasminogen activator (u-PA) in fibrin zymograms of both PC-3 and DU-145 cells and the expression of u-PA mRNA of PC-3 cells. However, the growth of these tumor cells was not affected by chromogranin A fragment at any concentrations used in this study. These results indicate that chromogranin A fragment increased the invasive potential of both PC-3 and DU-145 cells probably through enhancement of cell motility and the production of u-PA.
Nagakawa
O
O
Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.
Murakami
K
K
Ogasawara
M
M
Murata
J
J
Fuse
H
H
Saiki
I
I
D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D053379:Chromogranin A; D002864:Chromogranins / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D015194:Diffusion Chambers, Culture; D004338:Drug Combinations; D005342:Fibrinolysis / Q000187:drug effects; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008297:Male; D009361:Neoplasm Invasiveness; D010446:Peptide Fragments / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D011509:Proteoglycans / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics
C506150:CHGA protein, human; D053379:Chromogranin A; D002864:Chromogranins; D004338:Drug Combinations; D007797:Laminin; D010446:Peptide Fragments; D011509:Proteoglycans; D012333:RNA, Messenger; C059485:matrigel; D003094:Collagen; D014568:Urokinase-Type Plasminogen Activator