In vivo fMRI demonstration of hypothalamic function following intraperitoneal glucose administration in a rat model.
The hypothalamic functional MRI (fMRI) response in an animal model was studied following energy intake. Six fasted (12 h) Sprague-Dawley (SD) rats were administered an intraperitoneal injection of glucose (0.72 grams/kg body weight), while a mid-sagittal slice through the hypothalamus was continuously imaged for 60 min using a conventional T2*-weighted gradient-echo sequence. All rats demonstrated a significant acute transient decrease in the fMRI signal intensity (mean: 3.4%) in the hypothalamic region within 12-16 min after intraperitoneal glucose injection. The SD rat may be a suitable model for future fMRI studies of the hypothalamus involving the administration of exogenous nutrients and medications.
Mahankali
S
S
Research Imaging Center, University of Texas Health Science Center, San Antonio 78284-6240, USA.
Liu
Y
Y
Pu
Y
Y
Wang
J
J
Chen
C W
CW
Fox
P T
PT
Gao
J H
JH
D000818:Animals; D016477:Artifacts; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007089:Image Enhancement / Q000379:methods*; D007274:Injections, Intraperitoneal; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D005947:Glucose