title: Characterization of an Azospirillum brasilense Tn5 mutant with enhanced N(2) fixation: the effect of ORF280 on nifH expression.
abstract: Disruption of an open reading frame (ORF) of 840 bp (280 amino acids; ORF280) in an Azospirillum brasilense Tn5 mutant resulted in a pleiotrophic phenotype. Besides an enhanced N(2)-fixing capacity and altered expression pattern of a nifH-gusA fusion, growth on the charged polar amino acids glutamate and arginine was severely affected. ORF280, similar to previously identified ORFs present in Bradyrhizobium japonicum (ORF277), Paracoccus denitrificans (ORF278) and Rhodobacter capsulatus (ORF277), exhibits in its C-terminus a significant similarity with the recently defined family of universal stress proteins.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Revers
authors.forename: L M
authors.initials: LM
authors.lastname: Passaglia
authors.forename: K
authors.initials: K
authors.lastname: Marchal
authors.forename: J
authors.initials: J
authors.lastname: Frazzon
authors.forename: C G
authors.initials: CG
authors.lastname: Blaha
authors.forename: J
authors.initials: J
authors.lastname: Vanderleyden
authors.forename: I S
authors.initials: IS
authors.lastname: Schrank
mesh_terms: D000595:Amino Acid Sequence; D015806:Azospirillum brasilense / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D005966:Glucuronidase / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics* / Q000502:physiology; D009591:Nitrogenase / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics*; D010088:Oxidoreductases; D020161:Physical Chromosome Mapping; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; D009591:Nitrogenase; C029130:nitrogenase reductase; D005966:Glucuronidase