title: Increase of external osmolarity reduces morphogenetic defects and accumulation of chitin in a gas1 mutant of Saccharomyces cerevisiae.
abstract: We have performed a physiological analysis of the effects of high-osmolarity media on gas1Delta cells. The reductions in the duplication time, number of pluribudded cells, hypersensitivity to Calcofluor and sodium dodecyl sulfate, and chitin level indicate a partial suppression of the mutant phenotype. GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway.
authors.affiliation: Dipartimento di Fisiologia e Biochimica Generali, Universitá degli Studi di Milano, 20133 Milan, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Turchini
authors.forename: L
authors.initials: L
authors.lastname: Ferrario
authors.forename: L
authors.initials: L
authors.lastname: Popolo
mesh_terms: D002686:Chitin / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D009994:Osmolar Concentration; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: C066709:GAS1 protein, S cerevisiae; D008562:Membrane Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D002686:Chitin; D012967:Sodium Dodecyl Sulfate; D011494:Protein Kinases; D020929:Mitogen-Activated Protein Kinase Kinases