5-HT5a receptors in the carotid body chemoreception pathway of rat.
By using a specific antibody, 5-HT5a receptor-like immunoreactivity was revealed in the chemoreceptive, oxygen sensitive, carotid body (CB) type I cells, and neurons of the petrosal ganglion (PG) and the superior cervical ganglion (SCG) in rat. mRNA encoding for the 5-HTa receptor was also detected in these tissues by RT-PCR, and confirmed with DNA sequencing. The present study provides direct evidence that 5-HT5a receptors are expressed in the CB, PG and SCG, which all likely play fundamental roles in arterial chemoreception.
Wang
Z Y
ZY
Department of Comparative Biosciences, University of Wisconsin-Madison, 53706, USA. zwang@svm.vetmed.wisc.edu
Keith
I M
IM
Beckman
M J
MJ
Brownfield
M S
MS
Vidruk
E H
EH
Bisgard
G E
GE
D000344:Afferent Pathways / Q000737:chemistry; D000818:Animals; D002344:Carotid Body / Q000737:chemistry* / Q000166:cytology; D015687:Cell Hypoxia; D002628:Chemoreceptor Cells / Q000737:chemistry*; D017950:Ganglia, Sensory / Q000737:chemistry*; D005930:Glossopharyngeal Nerve / Q000737:chemistry / Q000502:physiology; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011985:Receptors, Serotonin / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012701:Serotonin / Q000502:physiology; D017783:Superior Cervical Ganglion / Q000737:chemistry*
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; C078730:serotonin 5 receptor; D012701:Serotonin