title: Adaptive response in patients treated with 131I.
abstract: The aim of this study was to investigate whether an adaptive response (defined as the induction of radiation tolerance after a small dose of radiation) could be observed in peripheral blood lymphocytes of patients treated with 1311 for thyroid disease.
  
  
  METHODS
  For each patient, blood samples were taken immediately before and 1 wk after 131I administration. Each blood sample was divided into 3 fractions and the fractions were subsequently irradiated in vitro with 0, 0.5, and 1.0 Gy 60Co gamma-rays. After blood culture for 70 h, cells were harvested and stained with Romanowsky-Giemsa and micronuclei were counted in 1000 binucleated cells. The increase in micronuclei by the in vitro irradiation of the blood samples taken before and after therapy was compared. In this setup, an adaptive response is represented by a significant decrease of the in vitro induced micronucleus yield after therapy compared with that before therapy. The iodine therapy can be considered as an in vivo adaptation dose, after which the subsequent in vitro irradiation acts as a challenge dose. To investigate the reproducibility of the method, 2 subsequent blood samples of healthy volunteers were taken 7 d apart. Irradiation and cell culture were performed as described.
  
  
  RESULTS
  In 8 of 20 patients, a significant (P = 0.0002) decrease was found in the in vitro induced micronucleus yield in the blood sample taken 1 wk after 1311 administration compared with that of the blood sample taken before therapy. No significant (P > 0.1) differences were observed between these 8 patients and the other patients when the number of micronuclei induced in vivo by the iodine treatment and the resulting equivalent total body dose were compared. None of the control subjects showed a significant change in micronucleus yield after in vitro irradiation between both blood samples taken 1 wk apart.
  
  
  CONCLUSIONS
  The iodine treatment can act as an in vivo adaptation dose and can induce an adaptive response that is observed by a decrease of the cytogenetic damage in peripheral blood lymphocytes after in vitro irradiation as a challenge dose. A large interindividual difference was observed.
authors.affiliation: Department of Biomedical Physics and Radiation Protection, University of Gent, Belgium.
authors.forename: M A
authors.initials: MA
authors.lastname: Monsieurs
authors.forename: H M
authors.initials: HM
authors.lastname: Thierens
authors.forename: A M
authors.initials: AM
authors.lastname: Vral
authors.forename: C
authors.initials: C
authors.lastname: Van De Wiele
authors.forename: L I
authors.initials: LI
authors.lastname: De Ridder
authors.forename: R A
authors.initials: RA
authors.lastname: Dierckx
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D002869:Chromosome Aberrations; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D011836:Radiation Tolerance / Q000502:physiology*; D015203:Reproducibility of Results; D013959:Thyroid Diseases / Q000097:blood / Q000235:genetics / Q000532:radiotherapy*; D013971:Thyrotoxicosis / Q000097:blood / Q000532:radiotherapy
chemical_list: D003037:Cobalt Radioisotopes; D007457:Iodine Radioisotopes