Electroosmosis- and pressure-driven chromatography in chips using continuous beds.
The application range of microchips can be extended to any mode of chromatography by filling the narrow channels with continuous polymer beds, exemplified by electrochromatography and ion-exchange chromatography. "Wall effects" are eliminated by anchoring the bed to the wall of the channel, an arrangement which has the additional advantage that no frits to support the bed are required. The design of the equipment is based on a quartz chip with all auxiliary pieces (for example, electrode vessels and fluid transfer fittings) placed in a rack, which permits a flexibility of great importance for automation. The same resolution and van Deemter plots were obtained in experiments performed in fused-silica capillaries and in chips for both low-molecular-weight (alkyl phenones, antidepressants) and high-molecular-weight substances (proteins). A sample of uracil, phenol, and benzyl alcohol was separated by electrochromatography in less than 20 s.
Ericson
C
C
Department of Biochemistry, University of Uppsala, Sweden.
Holm
J
J
Ericson
T
T
Hjertén
S
S
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods*; D009995:Osmosis; D011108:Polymers; D011312:Pressure; D011791:Quartz
D011108:Polymers; D011791:Quartz