Comparative electrochemical behaviour of biotin hydrazide and photobiotin. Importance in the development of biosensors.
The cyclic voltammetric behaviour of biotin hydrazide and photobiotin on carbon paste electrodes has been studied. Biotin hydrazide presents an anodic and irreversible process, meanwhile photobiotin presents two, adsorptive in nature. This characteristic makes photobiotin desirable for following the interaction between biotin and streptavidin, being possible to detect a streptavidin concentration of 10(-12) M. The evidence of this reaction has been shown either directly in solution or on the electrode surface. Photobiotin as the molecule portable of analytical information and carbon paste as the solid support could be applied to the development of sensors based on the oxidation of this molecule.
Rubio-González
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C
Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.
Fernández-Abedul
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MT
Costa-García
A
A
D001386:Azides; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000031:analogs & derivatives*; D002244:Carbon; D004563:Electrochemistry; D004566:Electrodes; D005069:Evaluation Studies as Topic; D019809:Streptavidin
D001386:Azides; C053311:biotin hydrazide; D001710:Biotin; D002244:Carbon; D019809:Streptavidin; C045549:photobiotin