title: [Comparative study of the reactability of phosphoric acid residues in tRNA(Ser) and tRNA(Leu) from Thermus thermophilus].
abstract: The reactivity of phosphates in the Thermus thermophilus tRNA(Ser) (GCU) and tRNA(Leu) (CAG) was studied using the ethylnitrosourea modification. It was shown that phosphates of nucleotides 58-60 (T loop), 20-22 (D loop), and 48 (at the junction of the variable and T stems) were poorly modified in both tRNAs. The most pronounced differences in the reactivity were observed for phosphates at the junctions of the variable stem with T-stem (47q, 49) and anticodon stem (45). This indicates differences in orientations of the long variable arm relative to the backbone in the tRNAs studied.
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, Ukraine.
authors.forename: O P
authors.initials: OP
authors.lastname: Kovalenko
authors.forename: Z M
authors.initials: ZM
authors.lastname: Petrushenko
authors.forename: I A
authors.initials: IA
authors.lastname: Kriklivyĭ
authors.forename: A D
authors.initials: AD
authors.lastname: Iaremchuk
authors.forename: M A
authors.initials: MA
authors.lastname: Tukalo
mesh_terms: D000926:Anticodon; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010756:Phosphoric Acids / Q000737:chemistry*; D012356:RNA, Transfer, Leu / Q000737:chemistry* / Q000235:genetics; D012362:RNA, Transfer, Ser / Q000737:chemistry* / Q000235:genetics; D016963:Thermus thermophilus / Q000235:genetics*
chemical_list: D000926:Anticodon; D010756:Phosphoric Acids; D012356:RNA, Transfer, Leu; D012362:RNA, Transfer, Ser; C030242:phosphoric acid