title: Evaluation of selected parameters of the cytokine system in burn wounds in children.
abstract: Using immunohistochemical methods, we studied cellular infiltrations of burn wounds in children. The relative number of cells with cluster of differentiation 3 (CD3) CD68 molecules, cells with receptors for interleukin-2 (IL-2) and its receptor (IL-2R), IL-3, IL-3R, IL-6, IL-6R, tumor necrosis factor-alpha (TNF-alpha), TNF-alphaR, and cells with TNF-alpha molecules on their surface was studied. The relationships between these values were determined. The following results were observed: (1) A positive correlation between the relative numbers of CD68 cells and the percentage of cells with receptors for IL-2 (IL-2R) and TNF-alpha (TNF-alphaR); (2) A negative correlation between the relative numbers of CD3 cells and cells with receptors for IL-2 and TNF-alpha; (3) A strong negative correlation between the relative numbers of CD3 and CD68 cells; (4) A positive correlation between the percentage of cells with receptors for TNF-alpha and the size of the burn wound; and (5) A positive correlation between the relative numbers of cells with the TNF-alpha molecule on their surfaces and studied receptors for interleukins, i.e., IL-2R, IL-3R, IL-6R, and TNF-alphaR. These results indicate a key role for TNF-alpha and its receptor within the cytokine system in the course of the inflammatory reaction in burn wounds.
authors.affiliation: Clinic of Pediatric Surgery and Oncology, Institute of Pediatrics, Medical University of Lódz, Sporna 36/50, Lódz, Poland 91-738.
authors.forename: E
authors.initials: E
authors.lastname: Andrzejewska
authors.forename: H
authors.initials: H
authors.lastname: Niewiadomska
mesh_terms: D000293:Adolescent; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D017252:CD3 Complex / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D015375:Receptors, Interleukin-2 / Q000032:analysis; D016185:Receptors, Interleukin-3 / Q000032:analysis; D019947:Receptors, Interleukin-6 / Q000032:analysis; D018124:Receptors, Tumor Necrosis Factor / Q000032:analysis; D047888:Receptors, Tumor Necrosis Factor, Type I; D012867:Skin / Q000737:chemistry / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017252:CD3 Complex; D016207:Cytokines; D015375:Receptors, Interleukin-2; D016185:Receptors, Interleukin-3; D019947:Receptors, Interleukin-6; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha; C067980:CD68 antigen, human; D000097382:CD68 Molecule