[Impact of stressors on serogroup B neisseria meningitidis outer membrane proteins during cultivation in bioreactor].
The authors examined the antigenic complexes obtained from serogroup B Neisseria meningitidis strain 125 cells grown in the bioreactor to different phases of growth in the reaction of gel precipitation and counter immunophoresis in a periodical fashion. A set of serotypic antigens was shown to increase from 3 to 9 with cultural growth. Their largest quantities were seen in the cells grown till the end of the stationary growth phase, the concentration of high-molecular weight peptides rising from 19% (in the exponential growth phase cells) to 54% (in the stationary growth phase ones). The revealed regularities were evidenced by gel precipitation examinations of the sera from rabbits immunized with meningococci grown till different growth phases. At the same time, there were the largest quantities of serotypic antigenic precipitation bands when the sera obtained in the culture grown till the end of the stationary growth phase were used. It cannot be excluded that the results depend on the combined effects of several stressors on Neisseria meningitidis in the transition from the exponential to stationary growth phase, as resulted from the increased synthesis of high-molecular weight proteins that seem to act as protectors.
Aleksakhina
N N
NN
Basnak'ian
I A
IA
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D019149:Bioreactors; D015169:Colony Count, Microbial; D009345:Neisseria meningitidis / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D012703:Serotyping; D013312:Stress, Physiological
D001425:Bacterial Outer Membrane Proteins