Phenotypic and functional properties of gamma delta T cells from patients with Guillain Barré syndrome.
In this study we have examined the phenotypic and functional properties of circulating gamma delta T cells in patients with Guillain Barre syndrome (GBS), in normal healthy controls, and in patients with active multiple sclerosis (MS). Cells expressing the Vdelta2 T cell receptor showed elevated expression of the C-lectin receptor NKRP1A in both GBS and MS, suggestive of an activated state. However, in patients with GBS these cells failed to respond to pyrenil-pyrophosphate derivatives and Vdelta2 + T cell clones derived from these patients released lower levels of IFNgamma than Vdelta2 + clones derived from controls and MS patients. In contrast, in patients with GBS the Vdelta1 + subset was expanded, showed elevated expression of NKRPIA and Vdelta1 + clones derived from these patients secreted high levels of IL-4. Our findings of expanded NKRP-1A +, IL-4-producing Vdelta1 T cells in the GBS patients suggests the possibility that these cells are activated by the recognition of non-protein antigens in an MHC-unrestricted manner and contribute to the humoral response to glycolipids that is a hallmark of this disease.
Borsellino
G
G
Laboratory of Neuroimmunology, IRCCS Santa Lucia, Rome, Italy.
Poccia
F
F
Placido
R
R
Tramonti
D
D
Mancino
G
G
Luchetti
S
S
Galgani
S
S
Bonetti
B
B
Bach
S
S
Cipriani
B
B
Brosnan
C F
CF
Battistini
L
L
D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism; D001773:Blood Cells / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D020275:Guillain-Barre Syndrome / Q000097:blood*; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism; D037181:Lectins, C-Type; D008024:Ligands; D009103:Multiple Sclerosis / Q000097:blood; D055651:NK Cell Lectin-Like Receptor Subfamily B; D010641:Phenotype; D010766:Phosphorylation; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism; D055607:Receptors, Natural Killer Cell; D012016:Reference Values; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology*
D000954:Antigens, Surface; D016207:Cytokines; C527203:KLRB1 protein, human; D037181:Lectins, C-Type; D008024:Ligands; D055651:NK Cell Lectin-Like Receptor Subfamily B; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D055607:Receptors, Natural Killer Cell