Mouse homolog of the Drosophila Pc-G gene esc exerts a dominant negative effect in Drosophila.
The Polycomb group genes are involved in maintaining long term transcriptional repression of the homeotic genes in both Drosophila and mammals. The mouse eed locus encodes the highly conserved ortholog of the Drosophila ESC protein. To test the functional conservation between the two genes, eed was introduced into the fly to determine whether it could rescue the esc mutant phenotype. eed exerted a dominant negative effect on the leg transformation phenotype associated with the esc mutation. This result is interpreted in light of in vitro protein-protein binding data and in vivo polytene chromosome staining indicating the lack of significant interaction between Eed and fly E(Z), a molecular partner of ESC. genesis 26:67-76, 2000
Wang
J
J
Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106-4955, USA.
Tie
F
F
Jane
E
E
Schumacher
A
A
Harte
P J
PJ
Magnuson
T
T
D000818:Animals; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000648:ultrastructure; D005121:Extremities / Q000196:embryology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005799:Genes, Dominant; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D011495:Histone-Lysine N-Methyltransferase; D019476:Insect Proteins / Q000235:genetics; D007866:Leg / Q000196:embryology; D046911:Macromolecular Substances; D008297:Male; D051379:Mice / Q000235:genetics*; D009024:Morphogenesis / Q000235:genetics; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D063150:Polycomb Repressive Complex 1; D063151:Polycomb Repressive Complex 2; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000502:physiology; D012097:Repressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
D029721:Drosophila Proteins; C107275:Eed protein, mouse; D019476:Insect Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; C066770:Pc protein, Drosophila; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C083329:E(z) protein, Drosophila; D011495:Histone-Lysine N-Methyltransferase; D063151:Polycomb Repressive Complex 2; C543389:esc protein, Drosophila; D063150:Polycomb Repressive Complex 1