Bacterial bioluminescence: isolation and expression of the luciferase genes from Vibrio harveyi.
Genes for the luciferase enzyme of Vibrio harveyi were isolated in Escherichia coli by a general method in which nonluminous, transposon insertion mutants were used. Conditions necessary for light production in E. coli were examined. Stimulation of transcription of the genes for luciferase (lux A and lux B) was required for efficient synethesis of luciferase. To enhance transcription bacteriophage promoter elements were coupled to the cloned lux gene fragments.
Belas
R
R
Agouron Institute, 505 Coast Boulevard South, La Jolla 92037, California, USA.
Mileham
A
A
Cohn
D
D
Hilman
M
M
Simon
M
M
Silverman
M
M
D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D008163:Luminescent Measurements; D014733:Vibrio / Q000201:enzymology / Q000235:genetics* / Q000502:physiology
D008156:Luciferases