Cloning and expression in Escherichia coli of 2-hydroxypropylphosphonic acid epoxidase from the fosfomycin-producing organism, Pseudomonas syringae PB-5123.
The fosfomycin resistance gene, fosC, has been cloned from the fosfomycin-producing organism, Pseudomonas syringae PB-5123. Sequence analysis upstream of this gene found a new ORF showing significant homology to 2-hydroxypropylphosphonic acid epoxidase from fosfomycin-producing Streptomyces wedmorensis. The purified recombinant protein of this ORF converted 2-hydroxypropylphosphonic acid to fosfomycin. This result clearly showed the ORF to encode 2-hydroxypropylphosphonic acid epoxidase in PB-5123.
Kuzuyama
T
T
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Seki
T
T
Kobayashi
S
S
Hidaka
T
T
Seto
H
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli; D005578:Fosfomycin / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D011549:Pseudomonas / Q000201:enzymology*
D001426:Bacterial Proteins; C096890:FosC protein, Pseudomonas syringae; D005578:Fosfomycin; D010088:Oxidoreductases; C405402:2-hydroxypropylphosphonic acid epoxidase