Acute effects of oral calcium load on parathyroid function and on bone resorption in young men.
OBJECTIVE
The aim of the present study was to check whether a calcium oral load was able to inhibit bone resorption as assessed by urinary excretion of a new bone marker, type 1 collagen cross-linked C-telopeptide (CrossLaps(TM)), in healthy young male adults.


METHODS
Twenty healthy young male adults (age 22 +/- 2 years) were studied. In one series of assays, an oral calcium load of 1 g of elemental calcium as calcium citrate dissolved in 200 ml of low-calcium water was ingested, while in another series of assays the subjects ingested 200 ml of water alone. Blood samples were collected before and 1, 2, 3 and 4 h after the intake of calcium. Urine was collected at 2-hour intervals, i.e. before and for 4 h after the intake of calcium. Serum ionized calcium, phosphate and intact parathormone (iPTH) were measured at each time point. Urinary calcium, phosphate, creatinine and CrossLaps (as a ratio to creatinine) were measured in each urine sample.


RESULTS
Calcium intake was associated with very significant (ANOVA, p < 0.001) increases in serum ionized calcium and decreases in PTH. After calcium intake, measurements of urinary CrossLaps showed a progressive statistically significant (ANOVA, p < 0.001) decrease (-20% at 2 h and -55% at 4 h), whereas after ingestion of water, the changes were modest and not statistically significant.


CONCLUSIONS
The present results show that bone resorption as assessed by urinary excretion of CrossLaps can be significantly suppressed by the ingestion of a 1-gram calcium load and attest that calcium supplementation has an acute effect on bone metabolism.
Guillemant
J
J
Service de Biochimie Medicale, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Le
H
H
Maria
A
A
Guillemant
S
S
D000284:Administration, Oral; D000328:Adult; D015415:Biomarkers / Q000652:urine; D001862:Bone Resorption / Q000652:urine*; D019355:Calcium Citrate / Q000494:pharmacology*; D003094:Collagen / Q000652:urine; D006801:Humans; D008297:Male; D010280:Parathyroid Glands / Q000187:drug effects* / Q000502:physiology; D010281:Parathyroid Hormone / Q000378:metabolism; D010446:Peptide Fragments / Q000652:urine
D015415:Biomarkers; D010281:Parathyroid Hormone; D010446:Peptide Fragments; C110762:glutamyl-lysyl-alanyl-histidyl-aspartyl-glycyl-glycyl-arginine; D003094:Collagen; D019355:Calcium Citrate