Morphometric and biochemical characteristics of short-term effects of ethanol on rat cardiac muscle.
Alcoholism is a very important cause of congestive cardiomyopathy in man. The aim of this study was to examine a short-term effect of ethanol in rat cardiac muscle, using histologic, morphometric and biochemical methods. Experiments were carried out in Wistar male albino rats, divided into two groups: the control group consisting of eight animals receiving tap water, and the experimental group comprising eight animals received ethyl alcohol for ten days, in a single daily dose of 3 g ethanol/kg body weight, per os, using esophageal intubation. The mean volume weighted nuclear volume of cardiac myocytes was estimated by point sampled intercept method, by objective x 100. The mean cubed nuclear intercept length was multiplied by pi and divided by 3. For biochemical analysis, a 10% water tissue homogenate from the left ventricle was made. In the experimental group, the mean volume-weighted nuclear volume (15.08 +/- 5.20 microm3) was significantly lower than in the control group (51.32 +/- 7.83 microm3) (p < 0.001). The treatment of experimental animals with ethanol caused significant increase of aldolase (p < 0.0001) and aspartate transaminase (p < 0.05) activity in the rat cardiac tissue; at the same time, the enzyme activity of creatine phosphokinase, alanine transaminase and alkaline phosphatase were not changed in the experimental group compared to the control values. The amount of the glucose in the cardiac muscle was greater in the experimental group compared to the control animals. Our results suggest that there is depression of cardiomyocyte nuclei in experimental animals treated with ethanol. Alcohol intake results in the loss of Krebs cycle enzymes and as a consequence there is greater utilization of fatty acids for energy production.
Mihailović
D
D
Institute of Pathology, University of Nis, Serbia, Yugoslavia. mihapat@kalca.junis.ni.ac.yu
Nikolić
J
J
Bjelaković
B B
BB
Stanković
B N
BN
Bjelaković
G
G
D000437:Alcoholism; D000818:Animals; D001699:Biometry; D002492:Central Nervous System Depressants / Q000633:toxicity*; D002952:Citric Acid Cycle / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005227:Fatty Acids / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar
D002492:Central Nervous System Depressants; D005227:Fatty Acids; D000431:Ethanol