Effect of ischemia-reperfusion on the heterogeneous lobular distribution pattern of glycogen content and glucose-6-phosphatase activity in human liver allograft.
In order to examine glucose metabolism in liver grafts after cold ischemia and reperfusion, the heterogeneous lobular distribution pattern of glycogen content and glucose-6-phosphatase activity was studied using histochemical methods. The characteristic heterogeneous lobular distribution pattern of glycogen and glucose-6-phosphatase was maintained after preservation and reperfusion. However, it appeared that glycogen content decreased in both periportal and centrilobular hepatocytes after reperfusion. The glycogen decrease was higher in periportal hepatocytes. Glucose-6-phosphatase activity was maintained after reperfusion in most of the cases in periportal hepatocytes. In centrilobular hepatocytes, more cases showed a decrease in enzyme activity. It is suggested that ischemia-reperfusion mainly affects the glycogen content in both periportal and centrilobular hepatocytes and that centrilobular glucose-6-phosphatase activity is more sensitive to ischemia-reperfusion injury than periportal hepatocytes.
Dodero
F
F
Laboratory of Cellular and Metabolic Pathology of Liver, INSERM U 260, Faculty of Medicine, Marseille, France.
Benkoel
L
L
Hardwigsen
J
J
Campan
P
P
Lambert
R
R
Botta-Fridlund
D
D
Le Treut
Y P
YP
Chamlian
A
A
D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002648:Child; D015925:Cryopreservation; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D006801:Humans; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation / Q000502:physiology*; D008875:Middle Aged; D015427:Reperfusion Injury / Q000378:metabolism*; D014184:Transplantation, Homologous
D006003:Glycogen; D005952:Glucose-6-Phosphatase