Identification of myelinated motor and sensory axons in a regenerating mixed nerve.
The common peroneal nerves of Wistar rats were transected and repaired to compare the sequential changes in the numbers of regenerating motor and sensory myelinated axons in a single mixed nerve. At sequential intervals (2, 4, and 12 weeks) after nerve repair, 3 kinds of staining were performed: cholinesterase staining (Karnovsky's staining) for motor axons, carbonic anhydrase staining for sensory axons, and antineurofilament immunohistochemical staining for all axons. At 2 weeks there was a large number of carbonic anhydrase-positive axons (600 +/- 98; mean +/- SD) and cholinesterase-positive axons were occasionally seen. Subsequently, there was a striking increase of cholinesterase-positive myelinated axons, reaching to 302 +/- 50 at 12 weeks. The results suggest that the myelinated sensory axons regenerate faster in the early stage of nerve regeneration and that regeneration of the myelinated motor axons is prominent in the subsequent stage. (J Hand Surg 2000; 25A:104-111.
Kawasaki
Y
Y
Department of Plastic, Reconstructive, and Aesthetic Surgery, University of Tokyo, Tokyo, Japan.
Yoshimura
K
K
Harii
K
K
Park
S
S
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000502:physiology*; D002256:Carbonic Anhydrases / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D009413:Nerve Fibers, Myelinated / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology*; D016900:Neurofilament Proteins / Q000378:metabolism; D009475:Neurons, Afferent / Q000502:physiology*; D010543:Peroneal Nerve / Q000293:injuries / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D016900:Neurofilament Proteins; D000110:Acetylcholinesterase; D002256:Carbonic Anhydrases