Synthetic profiles of polypeptides of human oocytes and normal and abnormal preimplantation embryos.
There is considerable variation in the rate of development in vitro of individual preimplantation human embryos. The relationship between the rate of development and patterns of polypeptide synthesis in individual embryos was examined using SDS-PAGE and autoradiography. After incubation in [35S]methionine, 19 polypeptide bands were identified that change between fertilization and the morula stage. Although changes in two of the bands occurred in embryos that were developing normally and in ageing oocytes, and are thus independent of fertilization, the changes identified in the remaining 17 bands occurred only after fertilization. In embryos that were developing abnormally, as assessed by delayed cleavage, cleavage arrest or extensive fragmentation, the alteration in polypeptide synthetic profiles increased with increasing abnormality.
Capmany
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G
Department of Obstetrics and Gynaecology, King's College School of Medicine and Dentistry, London, UK.
Bolton
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VN
D001345:Autoradiography; D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008715:Methionine / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D010452:Peptide Biosynthesis
D008715:Methionine