Indigenous plasmids in a production line of strains for penicillin G acylase derived from Escherichia coli W.
Three indigenous plasmids designated pRK1, pRK2 and pRK3 were identified among producers of penicillin G acylase (PGA) derived from the strain Escherichia coli W ATCC 9637. Their size and copy number (CN) in E. coli W were determined (kb; CN): pRK1 (80; 3.4), pRK2 (5.1; 71), and pRK3 (4.8; 13.7). Strain E. coli RE2 harboring these plasmids was used for selection of strains with reduced number of plasmids: the strain RE3 without plasmid pRK1 and the plasmid-less strain cERE3 were isolated. Indigenous plasmids did not code for the resistance determinants against 23 antibiotics and 10 heavy metals.
Sobotková
L
L
Laboratory of Enzyme Technology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Grafková
J
J
Stĕpánek
V
V
Vacík
T
T
Maresová
H
H
Kyslík
P
P
D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D007218:Industrial Microbiology; D008826:Microbial Sensitivity Tests; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000235:genetics; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins; D010399:Penicillin Amidase