title: High throughput analysis of 10 microsatellite and 11 diallelic polymorphisms on the human Y-chromosome.
abstract: We describe an integrated approach to the determination of complex Y chromosome haplotypes that is both fast and relatively inexpensive. The method employs GeneScan technology to enable a researcher to assay repeat number variation at ten microsatellite loci and determine the status of 11 diallelic polymorphisms. The method requires only four PCRs and four GeneScan runs per sample and is relatively insensitive to sample DNA concentration.
authors.affiliation: The Centre for Genetic Anthropology, The Department of Anthropology, University College London, UK. m.thomas@ucl.ac.uk
authors.forename: M G
authors.initials: MG
authors.lastname: Thomas
authors.forename: N
authors.initials: N
authors.lastname: Bradman
authors.forename: H M
authors.initials: HM
authors.lastname: Flinn
mesh_terms: D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D011933:Reagent Kits, Diagnostic