Molecular cloning and functional expression of Ca(v)3.1c, a T-type calcium channel from human brain.
Low voltage-activated T-type calcium channels are encoded by a family of at least three genes, with additional diversity created by alternative splicing. This study describes the cloning of the human brain alpha1G, which is a novel isoform, Ca(v)3.1c. Comparison of this sequence to genomic sequences deposited in the GenBank allowed us to identify the intron/exon boundaries of the human CACNA1G gene. A full-length cDNA was constructed, then used to generate a stably-transfected mammalian cell line. The resulting currents were analyzed for their voltage- and time-dependent properties. These properties identify this gene as encoding a T-type Ca(2+) channel.
Cribbs
L L
LL
Department of Physiology and the Cardiovascular Institute, Loyola University Medical Center, Maywood, IL, USA.
Gomora
J C
JC
Daud
A N
AN
Lee
J H
JH
Perez-Reyes
E
E
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D020747:Calcium Channels, T-Type / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D006801:Humans; D007438:Introns; D007700:Kinetics; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D051381:Rats; D014162:Transfection
C121855:CACNA1G protein, human; C500994:Cacna1g protein, rat; D020747:Calcium Channels, T-Type; D017931:DNA Primers; D018076:DNA, Complementary