Characterization of two cDNA clones encoding isozymes of the F(1)F(0)-ATPase inhibitor protein of rice mitochondria.
Two cDNA clones encoding F(1)F(0)-ATPase inhibitor proteins, which are loosely associated with the F(1) part of the mitochondrial F(1)F(0)-ATPase, were characterized from rice (Oryza sativa L. cv. Nipponbare). A Northern hybridization showed that the two genes (designated as IF(1)-1 and IF(1)-2) are transcribed in all the organs examined. However, the steady-state mRNA levels varied among organs. A comparison of the deduced amino acid sequences of the two IF(1) genes and the amino acid sequence of the mature IF(1) protein from potato revealed that IF(1)-1 and IF(1)-2 have N-terminal extensions with features that are characteristic of a mitochondrial targeting signal. To determine the subcellular localization of the gene products, the IF(1)-1 or IF(1)-2 proteins were fused in frame to the green fluorescent protein (GFP) or the fused GFP-beta-glucuronidase, and expressed transiently in onion or dayflower epidermal cells. Localized fluorescence was detected in mitochondria, confirming that the two IF(1) proteins are targeted to mitochondria.
Nakazono
M
M
Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Imamura
T
T
Tsutsumi
N
N
Sasaki
T
T
Hirai
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D008164:Luminescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics*; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D000097795:ATPase Inhibitory Protein
D018076:DNA, Complementary; D049452:Green Fluorescent Proteins; D007527:Isoenzymes; D008164:Luminescent Proteins; D020033:Protein Isoforms; D011506:Proteins; D006180:Proton-Translocating ATPases; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D000097795:ATPase Inhibitory Protein