title: Effect of warm ischemia time and organ perfusion technique on liver microvascular preservation in a non-heart-beating rat model.
abstract: BACKGROUND
  Current organ shortage has led to a reconsideration of non-heart-beating cadaveric donation.
  
  
  METHODS
  We assessed the effectivity of dual, i.e., arterial and portal-venous versus exclusive, arterial gravity perfusion for procurement of rat livers after 30 min and 60 min of cardiac arrest, analyzing the rate and homogeneity of microvascular perfusion by in situ fluorescence microscopy.
  
  
  RESULTS
  After 30 min of cardiac arrest, a nearly 100% recovery of acinar perfusion with a sinusoidal density not significantly different from that of normal, nonischemic livers was achieved by dual gravity perfusion. Prolongation of cardiac arrest to 60 min caused an almost 50% deficit of acinar and sinusoidal perfusion (P<0.05) with a concomitant 2-3-fold increase of heterogeneity of hepatic microperfusion. Regardless of the warm ischemic time period, dually perfused livers exhibited significantly (P<0.05) higher rates of both acinar and sinusoidal perfusion with increased homogeneity of microcirculation when compared with exclusive arterial perfusion.
  
  
  CONCLUSIONS
  These data underline the need and benefit of dual perfusion as well as the limitation of warm ischemic tolerance to 30 min for safe liver procurement of non-heart-beating donors.
authors.affiliation: Institute for Clinical and Experimental Surgery, University of Saarland, Homburg/Saar, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Richter
authors.forename: J
authors.initials: J
authors.lastname: Yamauchi
authors.forename: T
authors.initials: T
authors.lastname: Minor
authors.forename: B
authors.initials: B
authors.lastname: Vollmar
authors.forename: M D
authors.initials: MD
authors.lastname: Menger
mesh_terms: D000818:Animals; D001158:Arteries; D001808:Blood Vessels / Q000473:pathology / Q000503:physiopathology; D005260:Female; D006324:Heart Arrest, Induced; D006358:Hot Temperature; D007511:Ischemia / Q000503:physiopathology*; D008102:Liver Circulation; D008297:Male; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D010477:Perfusion / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014680:Veins