title: A new member of the GTPase superfamily that is upregulated in highly metastatic cells.
abstract: Two sublines of B16 melanoma cells, F10 and BL6, are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. We found a new member of the GTPase superfamily, namely TIB929, which displayed an induction of expression in BL6 cells. It conserved three consensus sequences for GTP-binding site motifs and showed a significant homology to the yeast Gtr2 gene throughout the coding sequence. TIB929 was expressed ubiquitously in human tumor cells, with a marked expression in highly metastatic cells. TIB929 was mapped on mouse chromosome 4D, syntenic to human chromosome 1p. The results suggested an involvement of TIB929 in malignant progression.
authors.affiliation: Second Department of Pathology, Kobe University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakaji
authors.forename: T R
authors.initials: TR
authors.lastname: Kataoka
authors.forename: K
authors.initials: K
authors.lastname: Watabe
authors.forename: K
authors.initials: K
authors.lastname: Nishiyama
authors.forename: H
authors.initials: H
authors.lastname: Nojima
authors.forename: Y
authors.initials: Y
authors.lastname: Shimada
authors.forename: F
authors.initials: F
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Matsushima
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
authors.forename: Y
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Y
authors.initials: Y
authors.lastname: Kitamura
authors.forename: A
authors.initials: A
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Maeda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D020558:GTP Phosphohydrolases / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D008297:Male; D008546:Melanoma, Experimental / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D005810:Multigene Family; D009363:Neoplasm Proteins; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; C430722:RRAGC protein, human; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020559:Monomeric GTP-Binding Proteins