Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli.
Multiplex PCR assay (m-PCR) with three sets of primers was developed for simultaneous identification of Campylobacter jejuni and C. coli. Poultry faecal samples were enriched in Preston broth for 24 h and streaking on selective media was performed before and after enrichment. m-PCR was applied on bacterial cultures harvested from media plates. The data showed a selective effect of Preston broth which favoured the growth of C. coli. Identification of the species by the hippurate hydrolysis test and by the m-PCR was performed on 294 isolates of Campylobacter. The efficiency of the identification by the biochemical test is only 34% in comparison to 100% efficiency with the PCR. The use of our m-PCR in combination with the culture method allowed reliable detection and identification of C. jejuni and C. coli within 3-4 d.
Denis
M
M
Unité d'Hygiène et Qualité des Produits Avicoles et Porcins, Agence Française de Sécurité Sanitaire des Aliments, Ploufragan, France.
Soumet
C
C
Rivoal
K
K
Ermel
G
G
Blivet
D
D
Salvat
G
G
Colin
P
P
D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D017000:Campylobacter coli / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D002645:Chickens; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000382:microbiology; D006626:Hippurates / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D011201:Poultry Diseases / Q000382:microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D006626:Hippurates; C030514:hippuric acid