Stress mediated changes in expression of the pkaC gene, encoding the catalytic subunit of cAMP-dependent protein kinase, in Aspergillus niger.
The transcriptional regulation of the pkaC gene, encoding the catalytic subunit of cAMP-dependent protein kinase from Aspergillus niger, was analysed under different environmental conditions. Quantitative determination of pkaC transcript showed a significant decrease in concentration of specific mRNA immediately after a temperature, hypoosmotic and hyperosmotic shock followed by stimulated synthesis. The amount of pkaC mRNA as well as PKA enzymatic activity steadily decreased during the initial phase of growth in 15% sucrose medium while a slight increase was observed at the time of a change in morphology from bulbous cells to filamentous growth. Transcriptional alternation might be mediated by multiple putative stress elements in the promoter region of pkaC gene.
Bencina
M
M
Department of Biotechnology and Industrial Mycology, National Institute of Chemistry, Ljubljana, Slovenia.
Legisa
M
M
D001234:Aspergillus niger / Q000235:genetics* / Q000254:growth & development; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics*; D005285:Fermentation; D015870:Gene Expression; D005800:Genes, Fungal / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D009997:Osmotic Pressure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
D007527:Isoenzymes; D017868:Cyclic AMP-Dependent Protein Kinases