Down-regulation of human Galbeta1,3GalNAc/Galbeta1,4GlcNAc alpha2,3-sialyltransferase (hST3Gal IV) gene during differentiation of the HL-60 cell line.
We studied the regulation of the human Galbeta1, 3GalNAc/Galbeta1,4GlcNAc alpha2,3-sialyltransferase (hST3Gal IV) gene during HL-60 cell differentiation induced by dimethyl sulfoxide (DMSO), all trans-retinoic acid (ATRA), and phorbol myristate acetate (PMA). During differentiation, levels of hST3Gal IV mRNA dramatically decreased after 1 day of stimulation. Reverse-transcription PCR identified two mRNA isoforms, types B1 and BX, in HL-60 cells. The results of luciferase assays showed that the level of B3 promoter activity is high, whereas A1/2 and B2 promoter activities are low in HL-60 cells, suggesting that type B1, BX, and B3 mRNA isoforms are expressed in HL-60 cells. A luciferase assay identified a functional DNA portion within the proximal region of the B3 promoter that confers negative transcriptional regulation on the hST3Gal IV B3 promoter during HL-60 differentiation. These results suggest that this element plays a critical role in down-regulating the B3 promoter activity during HL-60 cell differentiation.
Taniguchi
A
A
Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho University, Funabashi, Chiba, Japan. taniaki@phar.toho-u.ac.jp
Hasegawa
Y
Y
Higai
K
K
Matsumoto
K
K
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D018922:HL-60 Cells; D006801:Humans; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012799:Sialyltransferases / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
D017931:DNA Primers; D012333:RNA, Messenger; D014212:Tretinoin; D012799:Sialyltransferases; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase; C000722087:ST3GAL1 protein, human