title: Danish patients with untreated multiple myeloma do not harbour human herpesvirus 8.
abstract: The role of human herpesvirus 8 (HHV-8) in multiple myeloma (MM) remains controversial. We examined 15 Danish MM patients before cytoreductive therapy. Mononuclear cells isolated from peripheral blood and bone marrow aspirates, as well as long-term cultured bone marrow stromal cells, were assayed for the presence of HHV-8 DNA. All material was tested by three simple unnested polymerase chain reaction (PCR) assays (amplifying regions of ORF26, ORFK1 and ORF75) and two nested PCR assays (amplifying regions of ORF26). HHV-8 was not demonstrated in any of the samples. Our findings do not suggest an association between HHV-8 and MM in the Danish population.
authors.affiliation: Department of Haematology, Aarhus University Hospital, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Rask
authors.forename: J
authors.initials: J
authors.lastname: Kelsen
authors.forename: G
authors.initials: G
authors.lastname: Olesen
authors.forename: J L
authors.initials: JL
authors.lastname: Nielsen
authors.forename: N
authors.initials: N
authors.lastname: Obel
authors.forename: N
authors.initials: N
authors.lastname: Abildgaard
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000302:isolation & purification*; D003718:Denmark; D005260:Female; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004279:DNA, Viral