Effect of sevoflurane on Ca2+ mobilization in Madin-Darby canine kidney cells.
We investigated the effect of the volatile anesthetic sevoflurane on Ca2+ signaling in Madin-Darby canine kidney (MDCK) cells by using the fluorescent dye fura-2/AM (1-[2-(5-carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methylphenoxy)-ethane-N,N,N,N-tetraacetic acid pentaace-toxymethyl ester) as the Ca2+ indicator. At a concentration of 0.15 mM, sevoflurane did not alter basal cytosolic free calcium concentration ([Ca2+]i); however, at concentrations of 0.45-0.6 mM, sevoflurane did elevate [Ca2+]i, mainly by releasing Ca2+ from the endoplasmic reticulum (ER) store. Sevoflurane (0.15 mM) did not change either the [Ca2+]i peak evoked by high doses of ATP or UTP or inhibition of the ER Ca2+ pump, although it did significantly slow down the decay of the [Ca2+]i rise. Lastly, sevoflurane inhibited the capacitative Ca2+ entry and Mn2+ quench of fura-2 fluorescence induced by Ca(2+)-mobilizing ligands.
Jan
C R
CR
Department of Medical Education and Research, Veterans General Hospital-Kaohsiung, Kaohsiung, Taiwan.
Wang
K Y
KY
Tseng
C J
CJ
D018685:Anesthetics, Inhalation / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002460:Cell Line; D004285:Dogs; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007668:Kidney; D008738:Methyl Ethers / Q000494:pharmacology*; D058910:Purinergic P2 Receptor Agonists; D000077149:Sevoflurane
D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D058910:Purinergic P2 Receptor Agonists; D000077149:Sevoflurane; D015544:Inositol 1,4,5-Trisphosphate; D000252:Calcium-Transporting ATPases; D002118:Calcium