Mucosal immunogenicity of plant lectins in mice.
The mucosal immunogenicity of a number of plant lectins with different sugar specificities was investigated in mice. Following intranasal (i.n.) or oral administration, the systemic and mucosal antibody responses elicited were compared with those induced by a potent mucosal immunogen (cholera toxin; CT) and a poorly immunogenic protein (ovalbumin; OVA). After three oral or i.n. doses of CT, high levels of specific serum antibodies were measured and specific IgA was detected in the serum, saliva, vaginal wash, nasal wash and gut wash of mice. Immunization with OVA elicited low titres of serum IgG but specific IgA was not detected in mucosal secretions. Both oral and i.n. delivery of all five plant lectins investigated ¿Viscum album (mistletoe lectin 1; ML-1), Lycospersicum esculentum (tomato lectin; LEA), Phaseolus vulgaris (PHA), Triticum vulgaris (wheat germ agglutinin (WGA), Ulex europaeus I (UEA-1) stimulated the production of specific serum IgG and IgA antibody after three i. n. or oral doses. Immunization with ML-1 induced high titres of serum IgG and IgA in addition to specific IgA in mucosal secretions. The response to orally delivered ML-1 was comparable to that induced by CT, although a 10-fold higher dose was administered. Immunization with LEA also induced high titres of serum IgG, particularly after i. n. delivery. Low specific IgA titres were also detected to LEA in mucosal secretions. Responses to PHA, WGA and UEA-1 were measured at a relatively low level in the serum, and little or no specific mucosal IgA was detected.
Lavelle
E C
EC
Rowett Research Institute, Bucksburn, Aberdeen, UK.
Grant
G
G
Pusztai
A
A
Pfüller
U
U
O'Hagan
D T
DT
D000281:Administration, Intranasal; D000284:Administration, Oral; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000494:pharmacology; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D037102:Lectins / Q000008:administration & dosage / Q000276:immunology* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins / Q000494:pharmacology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D037102:Lectins; D010835:Phytohemagglutinins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014909:Wheat Germ Agglutinins; D010047:Ovalbumin; D002772:Cholera Toxin; D006735:Horseradish Peroxidase