title: Lactococci as probiotic strains: adhesion to human enterocyte-like Caco-2 cells and tolerance to low pH and bile.
abstract: There have been few studies on the probiotic activity of Lactococcus strains although they are commonly used as starter bacteria in manufacturing many kinds of fermented dairy products. Nine strains of the genus Lactococcus were examined for their probiotic properties, such as adherence to human enterocyte-like Caco-2 cells and tolerance to acid and bile. Six strains were adhesive and the highest adhesion was observed with Lactcoccus lactis ssp. lactis NIAI527. This strain adhered to the microvilli of cells as observed by scanning electron microscopy and also tolerated low pH and bile. These properties should make strain 527 a potential new probiotic strain.
authors.affiliation: Department of Animal Products, National Institute of Animal Industry, Ibaraki, Japan. anne@niai.affrc.go.jp
authors.forename: H
authors.initials: H
authors.lastname: Kimoto
authors.forename: J
authors.initials: J
authors.lastname: Kurisaki
authors.forename: N M
authors.initials: NM
authors.lastname: Tsuji
authors.forename: S
authors.initials: S
authors.lastname: Ohmomo
authors.forename: T
authors.initials: T
authors.lastname: Okamoto
mesh_terms: D001422:Bacterial Adhesion; D001646:Bile; D018938:Caco-2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017045:Lactococcus / Q000187:drug effects / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D019936:Probiotics