Genetic mapping of the rat mutation creeping and evaluation of its positional candidate gene reelin.
We have previously described a rat autosomal recessive mutation, creeping (cre), causing severe ataxia and disarrangement of neuronal cells in the central nervous system. The mutant strain has recently been successfully inbred, named Komeda Zucker creeping (KZC) rat. In the present study, we have performed a genetic analysis of the creeping mutation, and mapped it to rat Chromosome (Chr) 4. Comparative mapping, together with the similarity of the phenotype, suggested that the creeping mutation is homologous to the mouse reeler mutation. In fact, reelin expression was markedly reduced in the homozygous mutant (cre/cre) animals compared with the normal littermates. Thus, the KZC rat should become a useful biological model with a novel mutation in the reelin gene.
Yokoi
N
N
Department of Medical Genetics (Novo Nordisk Pharma), Chiba University School of Medicine, Japan.
Shimizu
S
S
Ishibashi
K
K
Kitada
K
K
Iwama
H
H
Namae
M
M
Sugawara
M
M
Serikawa
T
T
Komeda
K
K
D000818:Animals; D015152:Blotting, Northern; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics* / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism / Q000473:pathology; D002874:Chromosome Mapping; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism; D006239:Haplotypes; D051379:Mice; D008818:Mice, Neurologic Mutants; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011924:Rats, Zucker; D000091042:Reelin Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000091042:Reelin Protein; C000717129:Reln protein, rat; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases