Interpretation of cell culture phenomena.
This paper discusses the dilemma of interpreting unusual or abnormal phenomena seen in cell cultures and is not intended to address the statistical design of experiments. Problems that can be encountered when growing cells in experimental situations include low or decreasing cell numbers, abnormal cell morphology, microbial contamination, and detachment of the cell monolayer. If any of these situations occur, it is not realistic to proceed with data analysis until the problem is corrected. The best policy is to attempt to standardize all types of cultures used for analysis and to avoid using any cultures that display atypical characteristics.
Vierck
J L
JL
Muscle Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, Washington 99164-1082, USA.
Dodson
M V
MV
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods* / Q000592:standards; D002478:Cells, Cultured / Q000166:cytology / Q000382:microbiology; D005069:Evaluation Studies as Topic; D009132:Muscles / Q000166:cytology; D012756:Sheep