A new quantitative in vitro for the detection of latex-specific IgE antibodies.
Immediate-type hypersensitivity to latex allergens has resulted in anaphylactic shock and death in numerous reported cases. The allergenic proteins of latex are contained within the natural rubber extract of Hevea brasiliensis and are eluted into the final product during the manufacturing process. The quantity and types of latex allergens found in different latex products depends on the manufacturing process. Not all of these allergens are available for use in the latex prick skin test, and as a result, such tests may not be conclusive. Furthermore, application of such allergens to the skin of undiagnosed hypersensitive individuals may have harmful effects on their health. Therefore, it is important to be able to utilize in vitro methods, which reliably identify latex allergy without placing hypersensitive individuals at risk. We have developed a relatively simple and new enzyme immuno-assay (EIA) method for the detection of latex allergy. This in vitro method is quantitative and allows for the classification of allergy to latex in a short time. In comparative studies, ninety-nine serum specimens with documented clinical history of latex allergy were tested by this method, and the results paralleled those of the skin prick test performed by an independent group. The data showed that the specificity and sensitivity of our assay approaches 97.5% and 100%, respectively. We conclude that, by using a simple assay, the detection of specific IgE to latex proteins may be valuable for screening individuals and for the diagnosis of allergy to latex.
Moussadeh
M
M
Hamedi
N
N
Alem
N
N
Alem
M
M
D000918:Antibody Specificity; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D007840:Latex / Q000276:immunology*; D020315:Latex Hypersensitivity / Q000097:blood* / Q000175:diagnosis; D012680:Sensitivity and Specificity; D012882:Skin Tests
D007840:Latex; D007073:Immunoglobulin E