title: Isolation and characterization of dcw cluster from Streptomyces collinus producing kirromycin.
abstract: A 4.5-kb BamHI fragment of chromosomal DNA of Streptomyces collinus containing gene ftsZ was cloned and sequenced. Upstream of ftsZ are localized genes ftsQ, murG, and ftsW, and downstream is yfiH. Gene ftsA is not adjacent to ftsZ or other genes of the cloned fragment. Protein FtsZ was isolated and characterized with respect to its binding to GTP and GTPase activity. The binding of GTP to FtsZ was Ca(2+) or Mg(2+) dependent with an optimum at 10 mM. The rate of GTP hydrolysis by FtsZ was stimulated by KCl. The presence of Ca(2+) (3-5 mM) resulted in a significant increase of GTPase activity. Higher concentrations of Ca(2+) than 5 mM had an inhibitory effect on GTPase activity. These results indicate that divalent ions (Ca(2+) or Mg(2+)) can be involved in regulation of GTP binding and hydrolysis of FtsZ. The maximum level of FtsZ was detected in aerial mycelium when spiral loops and sporulation septa were formed. FtsZ is degraded after finishing sporulation septa.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnská 1083, Prague 4, 142 20, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Mikulík
authors.forename: E
authors.initials: E
authors.lastname: Zhulanova
authors.forename: M
authors.initials: M
authors.lastname: Krátký
authors.forename: O
authors.initials: O
authors.lastname: Kofronová
authors.forename: O
authors.initials: O
authors.lastname: Benada
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D003598:Cytoskeletal Proteins; D004269:DNA, Bacterial / Q000032:analysis; D029968:Escherichia coli Proteins; D020558:GTP Phosphohydrolases / Q000378:metabolism; D005798:Genes, Bacterial; D006160:Guanosine Triphosphate / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D005810:Multigene Family; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry / Q000235:genetics; D011728:Pyridones / Q000378:metabolism; D017421:Sequence Analysis; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C068023:FtsQ protein, E coli; C071525:FtsZ protein, Bacteria; D008565:Membrane Proteins; D011728:Pyridones; C061684:FtsW protein, Bacteria; D006160:Guanosine Triphosphate; D017351:N-Acetylglucosaminyltransferases; C064057:UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; D020558:GTP Phosphohydrolases; C008536:mocimycin