Neostigmine influences the L-dopa-induced extracellular dopamine levels in the striatum.
Using in vivo microdialysis in freely moving rats, we show that the addition to the dialysis perfusion fluid of the acetylcholinesterase inhibitor neostigmine influences the decarboxylation of levodopa (L-dopa). Continuous perfusion of neostigmine (10, 50 and 100 nM) in striatum attenuated the L-dopa-induced dopamine release in a dose-dependent manner. This effect suggests that changes in magnitude of drug responses may occur when an acetylcholinesterase inhibitor is included in the perfusion solution. Results obtained under these circumstances should be carefully interpreted concerning the pharmacological effects of other drugs when used concomitantly with neostigmine.
Izurieta-Sánchez
P
P
Department of Pharmaceutical Chemistry, Pharmaceutical Institute, Vrije Universiteit Brussel, Laarbeeklaan, 103, B-1090, Brussels, Belgium.
Jonkers
N
N
Sarre
S
S
Ebinger
G
G
Michotte
Y
Y
D000818:Animals; D001711:Biotransformation; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D007980:Levodopa / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D009388:Neostigmine / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D009388:Neostigmine; D007980:Levodopa; D004298:Dopamine