Determination of saccharides in sake by high-performance liquid chromatography with polarized photometric detection.
A high-performance liquid chromatographic method has been developed for the determination of saccharides in sake, an alcoholic beverage brewed from rice. Saccharides in sake were separated on a normal phase (carbamoyl bonded silica) column using a linear gradient elution of water in acetonitrile. Seven saccharides, glucose, maltose, isomaltose, maltotriose, panose, isomaltotriose and ethyl alpha-D-glucoside, were determined by a polarized photometric detector. Unidentified peaks suggesting saccharides with polymerization degrees over 4 were also observed. The proposed method did not require any sample clean-up treatment. As an application, saccharide compositions in various kinds of sake were compared.
Hayakawa
K
K
Faculty of Pharmaceutical Sciences, Kanazawa University, 13-1, Takaramachi, Kanazawa 920-0934, Japan. hayakawa@dbs.p.kanazawa-u.ac.jp
Ando
K
K
Yoshida
N
N
Yamamoto
A
A
Matsunaga
A
A
Nishimura
M
M
Kitaoka
M
M
Matsui
K
K
D001628:Beverages / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010783:Photometry / Q000379:methods*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D002241:Carbohydrates