[Identification of the anti-rIL-1ra McAb].
Using dot-immuno-blot and Western blotting techniques, we have identified the specificity of anti-rIL-1ra McAbs. The results showed that the anti-rIL-1ra McAb 2E4 specifically reacted with rIL-1ra, from which the recognizing signal of dot-immunoblot was significantly positive (+2) and a single rIL-1ra protein band (20 kd) was clearly recognized by Western blotting. The control results of PcAb recognization have further proved the high sensitivity of Western blotting and high specificity of rIL-1ra McAb 2E4. The successful identification of rIL-1ra McAb 2E4 will be helpful to further understand the biologic effects, especially the pro-inflammatory properties, to study the change of rIL-1ra level during inflammation, and to instruct the clinical use of rIL-1ra.
Feng
P
P
Department of Immunology, School of Basic Medical Sciences, Chengdu.
Zhao
Z
Z
Zhang
C
C
Wei
D
D
Cai
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D015153:Blotting, Western; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins