Fluorescence diagnosis of endometriosis on the chorioallantoic membrane using 5-aminolaevulinic acid.
The chorioallantoic membrane (CAM) is a useful model for the fluorescence diagnosis of experimentally induced endometriosis. In our experimental setup 75.7% of the histologically examined tissue preparations were viable and only 24.3% showed signs of necrosis on the CAM after various periods of incubation. Best results were obtained when grafting to the CAM was performed between days 7 and 9 and when implants were left on the CAM for 3-5 days (P < 0.05). We were able to demonstrate that 5-aminolaevulinic acid (ALA) is stored selectively in ectopic endometrium. The subsequent fluorescence of the endometrium shows a rapid increase that reaches a peak after 10-14 h which can be clearly differentiated from the weaker fluorescence of grafted normal peritoneum and fimbriae (P < 0.01).
Malik
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E
Department of Obstetrics and Gynaecology, Medical University Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
Meyhöfer-Malik
A
A
Berg
C
C
Böhm
W
W
Kunzi-Rapp
K
K
Diedrich
K
K
Rück
A
A
D000328:Adult; D000482:Allantois / Q000378:metabolism* / Q000637:transplantation; D000622:Aminolevulinic Acid; D000818:Animals; D002642:Chick Embryo; D004715:Endometriosis / Q000175:diagnosis*; D004717:Endometrium / Q000098:blood supply / Q000166:cytology / Q000637:transplantation; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005453:Fluorescence; D006801:Humans; D018919:Neovascularization, Physiologic; D013997:Time Factors; D014183:Transplantation, Heterologous
D000622:Aminolevulinic Acid