title: Prevalence of hepatitis C virus antibodies and genotypes in asymptomatic, first-time blood donors in Namibia.
abstract: Reported is the prevalence of hepatitis C virus (HCV) in Namibia as determined using a third-generation enzyme-linked immunosorbent assay (ELISA) on samples of blood collected from all asymptomatic, first-time blood donors between 1 February and 31 July 1997 (n = 1941). The HCV seroprevalence was 0.9% (95% confidence interval (CI): 0.5-1.5%) and no associations were detected between a positive HCV serostatus and the person's sex, region of residence, or previous hepatitis B exposure or hepatitis B carrier status, as determined by hepatitis B surface antigen (HBsAg). The only significant association in a logistic regression model was an increase in HCV positivity with increasing age (P = 0.04). Viral RNA was amplified from 2 out of 18 (11.1%) specimens that were ELISA positive. Genotyping of these specimens, by restriction fragment length polymorphism (RFLP), showed the presence of genotypes 5 and 1a. The positive predictive value of using HBsAg positivity as a surrogate screening marker for HCV in Namibian blood donors was poor (1.6%), with low sensitivity (16.7%) and specificity (89.3%), and detecting only 3 out of 18 serologically HCV-positive specimens. The results of this first study of the prevalence and epidemiology of HCV infection in Namibia suggest that donor blood should be screened for HCV by ELISA in order to prevent the transmission of hepatitis C virus.
authors.affiliation: National Institute for Virology, University of Witwatersrand, Johannesburg, South Africa.
authors.forename: E
authors.initials: E
authors.lastname: Vardas
authors.forename: F
authors.initials: F
authors.lastname: Sitas
authors.forename: K
authors.initials: K
authors.lastname: Seidel
authors.forename: A
authors.initials: A
authors.lastname: Casteling
authors.forename: J
authors.initials: J
authors.lastname: Sim
mesh_terms: D000293:Adolescent; D000328:Adult; D001782:Blood Donors / Q000706:statistics & numerical data*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000276:immunology; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D008297:Male; D009276:Namibia / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D016036:Seroepidemiologic Studies
chemical_list: D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies