title: The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells.
abstract: The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.
authors.affiliation: Institute of Medical Microbiology and Immunology, The Panum Institute, Copenhagen, Denmark.
authors.forename: N
authors.initials: N
authors.lastname: Odum
authors.forename: S
authors.initials: S
authors.lastname: Bregenholt
authors.forename: K W
authors.initials: KW
authors.lastname: Eriksen
authors.forename: S
authors.initials: S
authors.lastname: Skov
authors.forename: L P
authors.initials: LP
authors.lastname: Ryder
authors.forename: K
authors.initials: K
authors.lastname: Bendtzen
authors.forename: R J
authors.initials: RJ
authors.lastname: Van Neerven
authors.forename: A
authors.initials: A
authors.lastname: Svejgaard
authors.forename: P
authors.initials: P
authors.lastname: Garred
mesh_terms: D015415:Biomarkers; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D019713:Receptors, CCR5 / Q000235:genetics* / Q000276:immunology*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology*
chemical_list: D015415:Biomarkers; D019713:Receptors, CCR5