title: The 2.0 A crystal structure of Thermus thermophilus methionyl-tRNA synthetase reveals two RNA-binding modules.
abstract: BACKGROUND
  The 20 aminoacyl-tRNA synthetases are divided into two classes, I and II. The 10 class I synthetases are considered to have in common the catalytic domain structure based on the Rossmann fold, which is totally different from the class II catalytic domain structure. The class I synthetases are further divided into three subclasses, a, b and c, according to sequence homology. No conserved structural features for tRNA recognition by class I synthetases have been established.
  
  
  RESULTS
  We determined the crystal structure of the class Ia methionyl-tRNA synthetase (MetRS) at 2.0 A resolution, using MetRS from an extreme thermophile, Thermus thermophilus HB8. The T. thermophilus MetRS structure is in full agreement with the biochemical and genetic data from Escherichia coli MetRS. The conserved 'anticodon-binding' residues are spatially clustered on an alpha-helix-bundle domain. The Rossmann-fold and anticodon-binding domains are connected by a beta-alpha-alpha-beta-alpha topology ('SC fold') domain that contains the class I specific KMSKS motif.
  
  
  CONCLUSIONS
  The alpha-helix-bundle domain identified in the MetRS structure is the signature of the class Ia enzymes, as it was also identified in the class Ia structures of the isoleucyl- and arginyl-tRNA synthetases. The beta-alpha-alpha-beta-alpha topology domain, which can now be identified in all known structures of the class Ia and Ib synthetases, is likely to dock with the inner side of the L-shaped tRNA, thereby positioning the anticodon stem.
authors.affiliation: Department of Chemistry, Faculty of Science, Ochanomizu University, Otsuka, Bunkyo-Ku, 112-8610, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Sugiura
authors.forename: O
authors.initials: O
authors.lastname: Nureki
authors.forename: Y
authors.initials: Y
authors.lastname: Ugaji-Yoshikawa
authors.forename: S
authors.initials: S
authors.lastname: Kuwabara
authors.forename: A
authors.initials: A
authors.lastname: Shimada
authors.forename: M
authors.initials: M
authors.lastname: Tateno
authors.forename: B
authors.initials: B
authors.lastname: Lorber
authors.forename: R
authors.initials: R
authors.lastname: Giegé
authors.forename: D
authors.initials: D
authors.lastname: Moras
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
authors.forename: M
authors.initials: M
authors.lastname: Konno
mesh_terms: D000926:Anticodon; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D008718:Methionine-tRNA Ligase / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D011487:Protein Conformation; D017510:Protein Folding; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D016963:Thermus thermophilus / Q000737:chemistry*
chemical_list: D000926:Anticodon; D016601:RNA-Binding Proteins; D008718:Methionine-tRNA Ligase