Determination of lamotrigine in human serum by liquid chromatography.
A rapid high-performance liquid chromatographic method was developed using a short silica column (30 mm x 4.6 mm) with an aqueous methanol mobile phase consisting of methanol-water-NH4H2PO4 (94:5.96:0.04) adjusted to a final apparent pH of 5.0 and pumped at a flow-rate of 1 ml/min. Ultraviolet detection was carried out at a wavelength of 280 nm, and serum samples were prepared for HPLC analysis by extraction into dichloromethane after basification. Lamotrigine was eluted at 0.96 min. Within-day variation of the method was 4.46% at 0.75 microg/ml and 2.37% at 6.0 microg/ml, and day-to-day variation was 9.10% at 0.75 microg/ml and 7.28% at 6.0 microg/ml.
Vidal
E
E
Biochemistry Service, Hospital General Universitario Vall d'Hebron, Barcelona, Spain. leonor@hg.vhebron.es
Pascual
C
C
Pou
L
L
D000927:Anticonvulsants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D000077213:Lamotrigine; D000432:Methanol; D008752:Methylene Chloride; D011786:Quality Control; D012680:Sensitivity and Specificity; D014227:Triazines / Q000097:blood*; D014867:Water
D000927:Anticonvulsants; D007202:Indicators and Reagents; D014227:Triazines; D014867:Water; D008752:Methylene Chloride; D000077213:Lamotrigine; D000432:Methanol