title: Comparison of Sarns 3M heparin bonded to Duraflo II and control circuits in a porcine model: macro- and microanalysis of thrombi accumulation in circuit arterial filters.
abstract: Heparin-bonded perfusion circuits have been reported to reduce the thrombus formation during various levels of systemic heparinization. The goal of this study was to compare the efficacy of thrombo-resistance of the Sarns 3M heparin-bonded circuit to Baxter Duraflo II and untreated control in a porcine model. Fifteen Yorkshire pigs (60-65 kg) were anesthetized, heparinized with 3000 IU, intravenously (i.v.) and surgically cannulated with an internal jugular outflow and a femoral vein inflow. All circuits consisted of a 22-Fr venous cannula, centrifugal pump, arterial filter, an 18-Fr cannula for return and connected with equal lengths of 3/8" polyvinyl chloride tubing. The flows were maintained at 2.0 l/min for 4 h. Thrombus formation in filter samples were morphometrically analyzed through macro-densitometry, light microscopy, and scanning electron microscopy (SEM). Our findings revealed that the 3M circuit had significantly less gross thrombus (p < 0.001), 66% and 84% less microscopic thrombi and fivefold less SEM-measured aggregates (p = 0.03) compared to the Duraflo II and uncoated groups. This study demonstrated that the 3M heparin-bonded circuit had significantly reduced the formation of micro- and macro-thrombi in the minimally heparinized pig model compared to the Duraflo II and untreated control circuits.
authors.affiliation: Department of Surgery, University Heart Center, University of Arizona, Tucson, USA.
authors.forename: D F
authors.initials: DF
authors.lastname: Larson
authors.forename: D
authors.initials: D
authors.lastname: Arzouman
authors.forename: L
authors.initials: L
authors.lastname: Kleinert
authors.forename: V
authors.initials: V
authors.lastname: Patula
authors.forename: S
authors.initials: S
authors.lastname: Williams
mesh_terms: D000818:Animals; D001803:Blood Transfusion / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible; D006493:Heparin; D013552:Swine; D013927:Thrombosis / Q000453:epidemiology / Q000473:pathology; D065227:Transfusion Reaction
chemical_list: D020099:Coated Materials, Biocompatible; D006493:Heparin