Human herpesvirus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients.
Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), has recently been identified within the bone marrow dendritic cells of multiple myeloma (MM) patients. This virus contains homologues to human cytokines such as IL-6 that could potentially stimulate myeloma cell growth and contribute to disease pathogenesis. Since mobilization chemotherapy may increase circulating dendritic cell numbers, we searched for HHV-8 in peripheral blood mononuclear cells (PBMCs) before and after mobilization chemotherapy given to MM patients. Furthermore, we determined if autograft purging using the CEPRATE SC device would reduce the percentage of HHV-8 infected stem cell products. Only two of the 39 PBMC samples collected prior to mobilization chemotherapy contained PCR detectable virus, yet nine of 37 PBMCs collected on the first day of leukapheresis had detectable HHV-8 (P = 0.016). HHV-8 was more frequently identified in autograft products before vs after Ceprate SC selection (40% vs 15%, P = 0.016). Although the role HHV-8 plays in myeloma pathogenesis remains unclear, these results imply that mobilization chemotherapy increases the numbers of circulating HHV-8-infected dendritic cells within the peripheral blood. In addition, CD34 selection of autograft products in MM patients may reduce the reintroduction of virally infected cells following high-dose chemotherapy. Bone Marrow Transplantation (2000) 25, 153-160.
Vescio
R A
RA
West LA VAMC/University of California, Los Angeles, CA 90073, USA.
Wu
C H
CH
Zheng
L
L
Sheen
D
D
Ma
H
H
Liu
J
J
Stewart
A K
AK
Ballester
O
O
Noga
S J
SJ
Rugo
H
H
Freytes
C
C
Stadtmauer
E
E
Sahebi
F
F
Tarantolo
S
S
Stiff
P
P
Schiller
G J
GJ
White
M
M
Jacobs
C
C
DiPersio
J
J
Anderson
K C
KC
Berenson
J R
JR
D018952:Antigens, CD34 / Q000032:analysis; D001483:Base Sequence; D001804:Blood Transfusion, Autologous; D016465:Bone Marrow Purging / Q000379:methods; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D003713:Dendritic Cells / Q000166:cytology / Q000821:virology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006566:Herpesviridae Infections / Q000097:blood / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007937:Leukapheresis; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000821:virology*; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000097:blood / Q000628:therapy* / Q000821:virology; D016133:Polymerase Chain Reaction; D015996:Survival Rate; D016896:Treatment Outcome; D019562:Viral Load
D018952:Antigens, CD34; D004279:DNA, Viral