Identification and characterization of a new allele for the beta subunit of follicle-stimulating hormone in Chinese pig breeds.
During evaluation of follicle-stimulating hormone-beta (FSHB) expression in anterior pituitary glands by an RNase protection assay (RPA), the expected fragment of 205 nucleotides at positions 759-963 was not detected in one boar that had moderate plasma and pituitary FSH concentrations. After subcloning and sequencing, mRNA from this boar lacked an 11-bp fragment (5'-CATTTGGAAAC-3') at nucleotide positions 807-817 of the 3'-untranslated region (3'-UTR, D allele). Wild-type FSHB (WT allele) was present in pituitary RNA and genomic DNA in both Meishan (MS) and White Composite (WC) pigs; whereas the D allele was present only in MS pigs (P < 0.01; 5/6 MS vs. 0/6 WC). Also, we found the D allele in five other Chinese breeds but absent in ten American Landrace, 11 Yorkshire and 17 Berkshire pigs. Additionally, the D allele had one silent nucleotide change in the coding region plus six, single nucleotide changes in the 3'-UTR.
Li
M D
MD
Department of Pharmacology, University of Tennessee Medical School, Memphis 38163, USA.
Rohrer
G A
GA
Wise
T H
TH
Ford
J J
JJ
D020413:3' Untranslated Regions; D000483:Alleles; D000818:Animals; D005640:Follicle Stimulating Hormone / Q000235:genetics* / Q000302:isolation & purification; D008040:Genetic Linkage; D008297:Male; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D016372:RNA, Antisense / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D013552:Swine / Q000235:genetics*
D020413:3' Untranslated Regions; D016372:RNA, Antisense; D005640:Follicle Stimulating Hormone