title: IL-10 contributes to the inhibition of contact hypersensitivity in mice treated with photodynamic therapy.
abstract: We have explored the effect of photodynamic therapy (PDT) with verteporfin on the induction and expression of contact hypersensitivity (CHS) to 2,4-dinitrofluorobenzene (DNFB) in normal mice and IL-10-deficient mice. Our results indicate that DNFB sensitized mice given PDT with verteporfin and whole body red light irradiation exhibited a significant reduction in CHS compared with control animals. Administration of rIL-12 reversed the effect(s) of PDT as did treatment of mice with anti-IL-10-neutralizing Ab. Knockout mice deficient in IL-10 were found to be resistant to the inhibitory effects of PDT. In vitro proliferative responses using spleen cells from DNFB-sensitized and PDT-treated mice showed a significantly lower response to DNBS as compared with cells from DNFB-sensitized mice or DNFB and PDT-treated IL-10-deficient mice. Finally, naive mice exposed to PDT exhibited an increase in skin IL-10 levels, which peaked between 72 and 120 h post-PDT. Together these data support the role of IL-10 as a key modulator in the inhibition of the CHS response by whole body PDT.
authors.affiliation: QLT PhotoTherapeutics Inc., Vancouver, BC, Canada. gsimkin@qlt-pdt.com
authors.forename: G O
authors.initials: GO
authors.lastname: Simkin
authors.forename: J S
authors.initials: JS
authors.lastname: Tao
authors.forename: J G
authors.initials: JG
authors.lastname: Levy
authors.forename: D W
authors.initials: DW
authors.lastname: Hunt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D003877:Dermatitis, Contact / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D004139:Dinitrofluorobenzene / Q000276:immunology; D004431:Ear, External / Q000276:immunology; D005260:Female; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010778:Photochemotherapy / Q000379:methods; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D016753:Interleukin-10; D004139:Dinitrofluorobenzene