Ovine adipose tissue monounsaturated fat content is correlated to depot-specific expression of the stearoyl-CoA desaturase gene.
The basis for the variation in fatty acid composition in different ovine adipose tissue depots was investigated. The proportion of stearic (C18:0) and oleic (C18:1) acids vary in a site-specific fashion; abdominal depots (omental and perirenal) contain relatively more C18:0 than C18:1, and carcass depots, especially sternum, have a markedly higher proportion of C18:1. Additionally, expression of a number of lipogenic enzyme genes (stearoyl-CoA desaturase [SCD], acetyl-CoA carboxylase-alpha [ACC-alpha], lipoprotein lipase [LPL]) and the cytoskeletal protein gene alpha-tubulin vary among depots, although the pattern of variation differs for each mRNA. When these expression data were related to the mean cell volume of adipocytes pooled from all depots, a significant pattern emerged: expression of the ACC-alpha, LPL, and alpha-tubulin genes was highly correlated with the size of adipocytes. In contrast, when the expression of SCD mRNA was assessed as a function of mean cell volume, two populations of adipocytes emerged: no significant correlation was found between the expression of SCD mRNA per adipocyte and mean cell volume for the abdominal depots, although a highly significant correlation was observed between SCD gene expression and mean cell volume for the carcass and epicardial depots. Similarly, a highly significant correlation was found for the amount of C18:1 per adipocyte and the abundance of SCD mRNA per adipocyte for the carcass and epicardial depots, whereas no significant correlation was observed for these traits for the omental and perirenal depots. Thus, the SCD gene seems to be regulated in a depot-specific fashion and in a manner distinct from that of the ACC and LPL genes.
Barber
M C
MC
Hannah Research Institute, Ayr, UK.
Ward
R J
RJ
Richards
S E
SE
Salter
A M
AM
Buttery
P J
PJ
Vernon
R G
RG
Travers
M T
MT
D000103:Acetyl-CoA Carboxylase / Q000235:genetics; D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000737:chemistry* / Q000201:enzymology*; D000818:Animals; D048429:Cell Size; D005229:Fatty Acids, Monounsaturated / Q000032:analysis*; D008071:Lipoprotein Lipase / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012756:Sheep / Q000378:metabolism*; D013230:Stearoyl-CoA Desaturase / Q000096:biosynthesis / Q000235:genetics*; D014404:Tubulin / Q000235:genetics
D005229:Fatty Acids, Monounsaturated; D012333:RNA, Messenger; D014404:Tubulin; D013230:Stearoyl-CoA Desaturase; D008071:Lipoprotein Lipase; D000103:Acetyl-CoA Carboxylase