Antimicrobial peptide of korean native goat lactoferrin and identification of the part essential for this activity.
The antimicrobial activity of lactoferrin isolated from Korean native goat (KN goat) milk was studied and its antimicrobial domain was identified using synthetic peptides. Antimicrobial activity was assayed by a micro-method using 96-well microplates and a microplate reader. The amino acid sequence of the antimicrobial domain was suggested to be YQWQRRMRKLGAPSIT and this sequence corresponds to amino acid residues 20 to 35 of KN goat lactoferrin. Five peptides with certain amino acid residues deleted were synthesized in an effort to identify the residues essential for antimicrobial activity and it was found that the part with the sequence RRMRK (24-28) is the region most important for this activity. On the other hand, the conformation of the peptides did not influence the antimicrobial activity.
Kimura
M
M
Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan.
Nam
M S
MS
Ohkouchi
Y
Y
Kumura
H
H
Shimazaki
K i
Ki
Yu
D Y
DY
D000595:Amino Acid Sequence; D000818:Animals; D006041:Goats; D007723:Korea; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology*; D008892:Milk / Q000737:chemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D010446:Peptide Fragments; D007781:Lactoferrin