title: [Effects of acetylcholine on inositol 1,4,5-triphosphate formation in cervix uteri myometrial cells of human].
abstract: OBJECTIVE
  To detect inositol 1,4,5-triphosphate (IP3) formation of pregnant and nonpregnant human myometrial cells induced by acetylcholine (ACh).
  
  
  METHODS
  [3H] IP3 competitive protein binding assay.
  
  
  RESULTS
  Basal levels of IP3 in pregnant and nonpregnant human myometrial cells were (82 +/- 9) and (96 +/- 10) nmol.g-1 (protein), respectively (n = 6). Incubated with ACh 50 mumol.L-1 for 5 min, IP3 reached the peak levels (109 +/- 11) and (122 +/- 15) nmol.g-1 (protein), respectively (n = 6), but difference of the increments of IP3 in pregnant and nonpregnant women was not significant. The increased IP3 induced by ACh was inhibited by atropine (Atr) 1 mumol.L-1.
  
  
  CONCLUSIONS
  The basal IP3 level in pregnant cervix myometrial cells was higher than that in nonpregnant women. ACh increased the IP3 formation.
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, China Medical University, Shenyang, China.
authors.forename: H
authors.initials: H
authors.lastname: Nie
authors.forename: M J
authors.initials: MJ
authors.lastname: Wei
authors.forename: M Q
authors.initials: MQ
authors.lastname: Gao
authors.forename: X P
authors.initials: XP
authors.lastname: Ding
authors.forename: Z
authors.initials: Z
authors.lastname: Li
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000328:Adult; D001285:Atropine / Q000494:pharmacology; D002469:Cell Separation; D005260:Female; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis*; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009215:Myometrium / Q000166:cytology / Q000378:metabolism*; D010121:Oxytocin / Q000494:pharmacology; D011247:Pregnancy / Q000378:metabolism*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D018727:Muscarinic Antagonists; D014665:Vasodilator Agents; D010121:Oxytocin; D001285:Atropine; D015544:Inositol 1,4,5-Trisphosphate; D000109:Acetylcholine