title: Phytochelatin homologs induced in hairy roots of horseradish.
abstract: When exposed to excess heavy metals, plants induce phytochelatins and related peptides (all designated as PCAs). Thus, when hairy roots of horseradish (Armoracia rusticana) were exposed for 3 days to cadmium (1 mM) along with reduced glutathione (2 mM), PCA induction occurred. Moreover, a new family of thiol peptides was detected as well as the previously known PCAs, as revealed by postcolumn-derivatization HPLC. Two were isolated and their structures were identified as (gamma-Glu-Cys)n-Gln (n = 3 and 4) by electrospray ionization-mass spectrometer spectra, this being confirmed by chemical synthesis of the peptides. These new analogs constitute the sixth PCA family identified to date.
authors.affiliation: National Institute of Health Sciences, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kubota
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Maitani
mesh_terms: D000595:Amino Acid Sequence; D002104:Cadmium / Q000494:pharmacology; D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione; D008667:Metalloproteins / Q000096:biosynthesis*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry*; D054811:Phytochelatins; D010940:Plant Proteins / Q000096:biosynthesis*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D010945:Plants, Edible / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D008667:Metalloproteins; D010455:Peptides; D010940:Plant Proteins; D002104:Cadmium; D054811:Phytochelatins; D005978:Glutathione