title: High-throughput and ultra-high-throughput screening: solution- and cell-based approaches.
abstract: The trend towards assay miniaturization for high-throughput and ultra-high-throughput screening continues to spur development of homogeneous, fluorescence-based assays in higher density, smaller volume microplate formats. Recently, first-generation microfluidic devices have been designed for performing continuous-flow biochemical and cell-based assays. These devices provide orders-of-magnitude reduction in reagent consumption, and offer the potential for implementing high-throughput screening in formats that integrate up-front compound handling with unique assay functionality.
authors.affiliation: Caliper Technologies Corporation, CA 94043, USA. steve. sundberg@calipertech.com
authors.forename: S A
authors.initials: SA
authors.lastname: Sundberg
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002477:Cells / Q000201:enzymology / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004798:Enzymes / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D008024:Ligands; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D011485:Protein Binding; D012996:Solutions
chemical_list: D004798:Enzymes; D008024:Ligands; D012996:Solutions