title: Apolipoprotein A-I localization and dipalmitoylphosphatidylcholine dynamics in reconstituted high density lipoproteins.
abstract: The structure and molecular dynamics of recombinant high density lipoproteins (rHDL) were studied by non-radiative energy transfer (NRET), fluorescence anisotropy and intensity measurements. The rHDL particles contained human plasma apolipoprotein (apo) A-I and dipalmitoylphosphatidylcholine (DPPC). Fluorescent cis- and trans-parinaric acids were used both as probes of molecular motion in the particle lipid phase and as acceptors in the Forster's energy transfer from apo A-I tryptophan residues to determine particle dimensions, apolipoprotein localization and lipid dynamics. The probes are sensitive to thermal wobbling (macromobility) and conformational deformations (micromobility) of phospholipid acyl chains. The experimental data fitted to various models of the particle structure are compatible with the following: (a) at T < Tt the particles appeared as lens-like discs with a radius of the lipid phase of 5 nm and a mean thickness of 4 nm, the value being more by 20% in the particle centre, the alpha-helices of about 1 nm thickness were located around the edge of the lipid core. Compared to liposomes, both macro- and micromobility of DPPC molecules in rHDL were more rapid due to a significant disorder of the boundary lipid molecules close to the apo A-I molecule. This disorder led to the increase of the specific surface area per one lipid molecule, S(o). The lipid phase can be divided into three regions: (i) zone I of the most tightly packed lipid (0-1.7 nm from the disc axis) with a S(o) value small as 0.5 nm2; (ii) intermediate zone II (from 1.7 to 4.0 nm); and (iii) boundary lipid zone III (4-5 nm) of significantly disordered lipid with a S(o) value large as 0.65 nm2. (b) at T> Tt the S(o) heterogeneity disappeared, the radius of the lipid phase did not increase significantly, not exceeding 5.2-5.4 nm, but protein-induced immobilization of lipid molecules which affected about half or more of the total lipid, became remarkable. The overall effect was the suppression of the transition amplitude in rHDL compared to liposomes. The structural inhomogeneity might underlie the function of the native plasma HDL as the key component of the transport and metabolism of plasma lipids.
authors.affiliation: National Research Centre for Preventive Medicine, Moscow, Russian Federation. dergunov@img.ras.ru
authors.forename: A D
authors.initials: AD
authors.lastname: Dergunov
authors.forename: G E
authors.initials: GE
authors.lastname: Dobretsov
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000097:blood / Q000737:chemistry*; D016632:Apolipoprotein A-I / Q000097:blood* / Q000737:chemistry*; D004735:Energy Transfer; D005231:Fatty Acids, Unsaturated; D005454:Fluorescence Polarization / Q000379:methods; D005456:Fluorescent Dyes; D006801:Humans; D008051:Lipid Bilayers / Q000097:blood / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D013816:Thermodynamics; D014364:Tryptophan
chemical_list: D016632:Apolipoprotein A-I; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D015060:1,2-Dipalmitoylphosphatidylcholine; D014364:Tryptophan; C013641:parinaric acid