title: [Detection of human cytomegalovirus by PCR in patients with gastrointestinal disease].
abstract: Human cytomegalovirus (HCMV) genomes was detected by PCR in 51 patients' biopsy or operation specimens with gastrointestinal disease. The positive rate was 9.80%. At the same time, 41 patients' serum HCMV-IgM was detected by indirect immunofluorescence assay (IFA); the positive rate was 46.34%. Patients with cancer and colitis exhibited a higher HCMV infection rate than those without cancer and colitis. The results suggest that PCR is sensitive and specific in detection of gastrointestinal HCMV infection, and the examination of biopsy or surgical specimen is superior than that of IFA of serum HMCV-IgG.
authors.affiliation: Department of Microbiology, Hunan Medical University, Changsha.
authors.forename: C
authors.initials: C
authors.lastname: Fan
authors.forename: K
authors.initials: K
authors.lastname: Shi
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000328:Adult; D000368:Aged; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000821:virology*; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral