[The dose-response of cell proliferation and secretion of CA125 II antigen to follicle stimulating hormone in ovarian cancer cell lines].
OBJECTIVE
To study the dose-response of the proliferation and secretion of CA125 II antigen with follicle stimulating hormone (FSH) in epithelial ovarian cancer cell lines.


METHODS
Two different kinds of ovarian cancer cell lines originated from different pathologic types-AO and 3AO were studied. Cancer cells were cultured in medium containing FSH in different concentrations. The proliferation rate of cells was detected by using the 3H-TdR intrusion technique. The accordant titer of CA125 II antigen in the culture medium was measured by enzyme linked immunoadsorbent assay technique.


RESULTS
Proliferation rate of cancer cells (AO and 3AO) was increased apparently as increasing in the concentration of FSH in the culture medium. A positive correlation was found between them (P < 0.05). Proliferation rate of AO cells positively correlated with secreting quantity of CA125 II antigen (P < 0.05), however, no such correlation was found in 3AO cells.


CONCLUSIONS
It suggested that FSH can stimulate the proliferation of epithelial ovarian cancer cell at high concentration and there is a accordant dose-response relationship between them. The secreting pattern of the CA125 II antigen varied by the pathological classification of the ovarian cancer.
Zhu
M
M
Obstetrics and Gynecology Hospital Shanghai Medical University.
Wu
Q
Q
Ge
B
B
D018394:CA-125 Antigen / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D018282:Cystadenocarcinoma, Mucinous / Q000276:immunology / Q000473:pathology; D018284:Cystadenocarcinoma, Serous / Q000276:immunology / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured
D018394:CA-125 Antigen; D005640:Follicle Stimulating Hormone