Cymbidium mosaic virus coat protein gene in antisense confers resistance to transgenic Nicotiana occidentalis.
The nucleotide sequence of the 3'-terminal region of the Korean isolate of cymbidium mosaic virus (CyMV-Ca) from a naturally infected cattleya was determined. The sequence contains an open reading frame (ORF) coding for the viral coat protein (CP) at the 3'-end and three other ORFs (triple gene block or movement protein) of CyMV. The CP gene encodes a polypeptide chain of 220 amino acids with a molecular mass of 23,760 Da. The deduced CP sequence showed a strong homology with those of two CyMVs reported. A construct of the CyMV-Ca CP gene in the antisense orientation in the plant expression vector pMBP1 was transferred via Agrobacterium tumefaciens-mediated transformation into Nicotiana occidentalis which is a propagation host of CyMV. The T1 progeny of the transgenic plants were inoculated with CyMV and found to be highly resistant to CyMV infection.
Lim
S H
SH
Division of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Ko
M K
MK
Lee
S J
SJ
La
Y J
YJ
Kim
B D
BD
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002213:Capsid / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000235:genetics*; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D036022:Capsid Proteins; D016373:DNA, Antisense; D012333:RNA, Messenger; C075095:coat protein, cymbidium mosaic virus