title: Distinct contributions of glycoprotein VI and alpha(2)beta(1) integrin to the induction of platelet protein tyrosine phosphorylation and aggregation.
abstract: Platelet activation by collagen depends principally on two receptors, alpha(2)beta(1) integrin (GPIa-IIa) and GPVI. During this activation, the nonreceptor protein tyrosine kinase pp72(syk) is rapidly phosphorylated, but the precise contribution of alpha(2)beta(1) integrin and GPVI to signaling for this phosphorylation is not clear. We have recently found that proteolysis of platelet alpha(2)beta(1) integrin by the snake venom metalloproteinase, jararhagin, results in inhibition of collagen-induced platelet aggregation and pp72(syk) phosphorylation. In order to verify whether the treatment of platelets with jararhagin had any effect on GPVI signaling, in this study we stimulated platelets treated with either jararhagin or anti-alpha(2)beta(1) antibody with two GPVI agonists, an antibody to GPVI and convulxin. Platelet shape change and phosphorylation of pp72(syk) by both GPVI agonists was preserved, as was the structure and function of GPVI shown by (125)I-labeled convulxin binding to immunoprecipitated GPVI from jararhagin-treated platelets. In contrast, defective platelet aggregation in response to GPVI agonists occurred in both jararhagin-treated and alpha(2)beta(1)-blocked platelets. This apparent cosignaling role of alpha(2)beta(1) integrin for platelet aggregation suggests the possibility of a topographical association of this integrin with GPVI. We found that both platelet alpha(2)beta(1) integrin and GPVI coimmunoprecipitated with alpha(IIb)beta(3) integrin. Since platelet aggregation requires activation of alpha(IIb)beta(3) integrin, defective aggregation in the absence of alpha(2)beta(1) suggests that this receptor may provide a signaling link between GPVI and alpha(IIb)beta(3). Our study therefore demonstrates that platelet signaling leading to pp72(syk) phosphorylation initiated with GPVI engagement by either convulxin or GPVI antibody does not depend on alpha(2)beta(1) integrin. However, alpha(IIb)beta(3) integrin may, in this model, require functional alpha(2)beta(1) integrin for its activation.
authors.affiliation: Department of Haematology, Royal Liverpool Hospital, Liverpool, United Kingdom. aurakami@liverpool.ac.uk
authors.forename: A S
authors.initials: AS
authors.lastname: Kamiguti
authors.forename: R D
authors.initials: RD
authors.lastname: Theakston
authors.forename: S P
authors.initials: SP
authors.lastname: Watson
authors.forename: C
authors.initials: C
authors.lastname: Bon
authors.forename: G D
authors.initials: GD
authors.lastname: Laing
authors.forename: M
authors.initials: M
authors.lastname: Zuzel
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D048429:Cell Size / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D003435:Crotalid Venoms / Q000494:pharmacology; D004792:Enzyme Precursors / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D016023:Integrins / Q000097:blood / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D008666:Metalloendopeptidases / Q000494:pharmacology; D019000:Phosphotyrosine / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000502:physiology; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000097:blood; D038981:Receptors, Collagen; D000072377:Syk Kinase; D000097146:Bothrops jararaca Venom
chemical_list: D000906:Antibodies; D003094:Collagen; D003435:Crotalid Venoms; D004792:Enzyme Precursors; D016023:Integrins; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D008666:Metalloendopeptidases; D019000:Phosphotyrosine; D010975:Platelet Aggregation Inhibitors; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins; D011505:Protein-Tyrosine Kinases; D038981:Receptors, Collagen; D000072377:Syk Kinase; D000097146:Bothrops jararaca Venom; C098341:platelet membrane glycoprotein VI; C028890:convulxin; C000606896:SYK protein, human