title: Surface-modification of polystyrene-microtitre plates via grafting of glycidylmethacrylate and coating of poly-glycidylmethacrylate.
abstract: Photografting of glycidylmethacrylate (GMA) onto commercially available polystyrene-microtitre plates was carried out in methanol as well as butanol with benzophenone (BP) as photoinitiator. Alternatively, prepolymers of polyglycidylmethacrylate (PGMA) were synthesized in methanol with azo-iso-butyrodinitrile (AIBN) as initiator and dip-coated onto polystyrene-microtitre plates. Both modification methods were tested in order to reach a high binding capacity for proteins. Peroxidase was used as model protein. In addition, the immobilization of myelin basic protein (MBP) to epoxy-modified microtitre plates is shown and a MBP-based ELISA has been developed.
authors.affiliation: Clinic of Craniomaxillofacial Surgery, Oral and Maxillofacial Surgery, Martin Luther University Halle Wittenberg, Germany.
authors.forename: A W
authors.initials: AW
authors.lastname: Eckert
authors.forename: D
authors.initials: D
authors.lastname: Gröbe
authors.forename: U
authors.initials: U
authors.lastname: Rothe
mesh_terms: D001672:Biocompatible Materials; D004797:Enzyme-Linked Immunosorbent Assay; D004852:Epoxy Compounds / Q000737:chemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D004676:Myelin Basic Protein / Q000032:analysis / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D011137:Polystyrenes; D012997:Solvents; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004852:Epoxy Compounds; D007202:Indicators and Reagents; D008689:Methacrylates; D004676:Myelin Basic Protein; D011109:Polymethacrylic Acids; D011137:Polystyrenes; D012997:Solvents; C042535:polyglycidyl methacrylate; C007870:glycidyl methacrylate