[Application of spectrophotometry to evaluation of the levels and the influence of chemotherapeutic drugs on ATP of malignant cell lines].
To evaluate the value of ATP spectrophotometry for malignant tumor in vitro chemosensitivity test, Hela and L929 cell lines, which were derived from human cervical carcinoma and mouse fibroma respectively, were investigated. The results showed that when the cellular numbers were > or = 2 x 10(5), the linearity between the ATP concentrations and the cellular numbers was observed, with all 2 cell types studied, and that when L929 cells were incubated with cis-platinum (1x, 2x, 5x x plasma peak concentration, PPC), a marked fall in ATP concentrations was observed and a clear dose-response curve was obtained, but no decrease of ATP concentrations was measured after incubation with adriamycin (1x, 2x, 5x x PPC). This study suggests that ATP spectrophotometry is a cheap, feasible method for studying the energy metabolism and the influence of chemotherapeutic drugs on ATP concentrations of malignant tumor.
Wang
H
H
Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chengdu.
Peng
Z
Z
Ding
S
S
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005350:Fibroma / Q000473:pathology*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D051379:Mice; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000970:Antineoplastic Agents; D004317:Doxorubicin; D000255:Adenosine Triphosphate; D002945:Cisplatin