Assignment of overlapping (1)H NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy.
The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key proton signals were strongly overlapped.
Kalic
M
M
Organisch-Chemisches Institut der Universität, Orléansring 23, Münster, 48149, Germany.
Lauterwein
J
J
Büsselmann
G
G
Kamp
G
G
D000818:Animals; D002347:Carps / Q000378:metabolism*; D018698:Glutamic Acid / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011522:Protons; D012661:Semen / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013654:Taurine / Q000737:chemistry
D011522:Protons; D013654:Taurine; D018698:Glutamic Acid; D011392:Proline; D008715:Methionine; C013111:methionine sulfoxide