Vasculogenesis in the day 6.5 to 9.5 mouse embryo.
The process of vasculogenesis was characterized in the 6.5- to 9.5-day mouse embryo and in allantoic culture by analysis of spatial and temporal expression patterns of the endothelial or hematopoietic lineage-associated proteins, TAL1, Flk1, platelet/endothelial cell adhesion molecule (PECAM), CD34, VE-cadherin, and Tie2. The study establishes that: (1) TAL1 and Flk1 are coexpressed in isolated mesodermal cells that give rise to endothelial cells and thus can be defined as angioblasts; (2) hematopoietic cells of blood islands express TAL1, but not Flk1; (3) vasculogenesis in the embryo proper is initiated by mesoderm fated to give rise to the endocardium; (4) the maturation/morphogenesis of blood vessels can be defined in terms of a sequential pattern of expression in which TAL1 and Flk1 are expressed first followed by PECAM, CD34, VE-cadherin, and later Tie2; and (5) TAL1 expression is down-regulated in endothelial cells of mature vessels. (Blood. 2000;95:1671-1679)
Drake
C J
CJ
Medical University of South Carolina, Department of Cell Biology and the Cardiovascular Developmental Biology Center, Charleston, SC 29425, USA. drakec@musc.edu
Fleming
P A
PA
D000482:Allantois / Q000166:cytology; D000818:Animals; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000096:biosynthesis / Q000235:genetics; D001011:Aorta / Q000196:embryology / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins; D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004622:Embryo, Mammalian / Q000098:blood supply; D004699:Endocardium / Q000196:embryology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000196:embryology / Q000378:metabolism; D005318:Fetal Heart / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D008648:Mesoderm / Q000166:cytology; D051379:Mice / Q000196:embryology*; D009024:Morphogenesis; D018919:Neovascularization, Physiologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics; D042787:Receptor, TIE-2; D017978:Receptors, Growth Factor / Q000096:biosynthesis / Q000235:genetics; D040262:Receptors, Vascular Endothelial Growth Factor; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; D000099152:Cadherin 5
D015703:Antigens, CD; D018952:Antigens, CD34; D051792:Basic Helix-Loop-Helix Proteins; D015820:Cadherins; D004268:DNA-Binding Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2; D017978:Receptors, Growth Factor; D040262:Receptors, Vascular Endothelial Growth Factor; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; D000099152:Cadherin 5; C489366:Tal1 protein, mouse