[Application of antigen linked with chemicals on nitrate cellulose membrane for dot-enzyme immunoassay].
HCMV-IgG in sera of pregnant women was detected by means of dot-enzyme immunoassay (DEIA), in which antigen was cross-linked on nitrate cellulose membrane through chemical reagent. The results showed better repetition by DEIA, compared with ELISA, and no significant differences were noted between the two methods in the rates of detecting HCMV-IgG in positive, weak positive and negative sera. The rate of agreement was 93.2%. In examining the sera which had been examined by ELISA for three times with the same, results the sensitivity, specificity, index and efficiency of diagnosis by DEIA were 100%, 96.6%, 196.6% and 98.7% respectively. Since this method is convenient, fast, of good repetition, economical and its diagnosis index meets the application criterion, it is more suitable for clinical laboratories.
Pan
P
P
Department of Laboratory Medicine, First Affiliated Hospital, Chengdu.
Yu
H
H
Zeng
R
R
Yang
J
J
D002482:Cellulose; D003587:Cytomegalovirus / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D011247:Pregnancy
D007074:Immunoglobulin G; D002482:Cellulose