title: Low frequency vibrations and structural characterization of a murine IgG2a monoclonal antibody studied by raman and IR spectroscopies.
abstract: The Raman and ir spectra of a murine IgG2a monoclonal antibody molecule are reported. In accordance with previous studies on immunoglobulins, the secondary structure is predominantly of the beta-sheet type. The low frequency region of the Raman spectrum was also analyzed in detail. A structured band with two maxima near 43 and 94 cm(-1) was observed. This band has been attributed to vibrations of elastic body. The results are interpreted using a simple elastic model.
authors.affiliation: Groupe de Recherche en Physique et Biophysique (GRPB), Université René Descartes, UFR Biomédicale, 45 Rue des Saints-Pères, 75270 Paris Cedex 06, France.
authors.forename: M
authors.initials: M
authors.lastname: Picquart
authors.forename: E
authors.initials: E
authors.lastname: Haro-Poniatowski
authors.forename: J F
authors.initials: JF
authors.lastname: Morhange
authors.forename: M
authors.initials: M
authors.lastname: Jouanne
authors.forename: M
authors.initials: M
authors.lastname: Kanehisa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G