Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay.
The single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.
Ward
T H
TH
Cancer Research Campaign Section of Drug Development and Imaging, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Marples
B
B
D052160:Benzothiazoles; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000528:radiation effects; D004249:DNA Damage; D003959:Diamines; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009930:Organic Chemicals; D011804:Quinolines; D014407:Tumor Cells, Cultured
D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA