title: A proteolipid protein-specific pre-mRNA (Ppm-1) contains intron 3 and is up-regulated during myelination in the CNS.
abstract: Alternative splicing of the precursor for messenger RNA (pre-mRNA) is a common process utilised by higher eukaryotes to modulate gene expression. A single primary transcript may generate several proteins with distinct functions, expressed in tissue-specific, developmental patterns. This article describes an oligodendrocyte-specific pre-mRNA product of proteolipid protein gene (P/p) transcription, which is the precursor for P/p but not Dm20 mRNA in the CNS. This P/p-specific pre-mRNA (Ppm-1) includes the intact intron 3 of the P/p gene. It is first expressed during active myelination, and it localises to the nucleus of oligodendrocytes, in both normal and jimpy (jp) murine CNS. In addition to mouse, Ppm-1 is found also in rat and dog, but not toad or trout. Our work suggests that alternative splicing of the P/p gene primary transcript follows a branching pattern, resulting in the presence of at least one P/p isoform-specific pre-mRNA molecule, Ppm-1. Therefore, Dm20 mRNA may be the product of a divergent set of pre-mRNA splicing events.
authors.affiliation: Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Scotland. D.Vouyioukus@vet.gla.ac.uk
authors.forename: D A
authors.initials: DA
authors.lastname: Vouyiouklis
authors.forename: J A
authors.initials: JA
authors.lastname: Barrie
authors.forename: I R
authors.initials: IR
authors.lastname: Griffiths
authors.forename: C E
authors.initials: CE
authors.lastname: Thomson
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000235:genetics; D001483:Base Sequence / Q000235:genetics; D001921:Brain / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D004274:DNA, Recombinant; D004285:Dogs; D029941:Fish Proteins; D018507:Gene Expression Regulation, Developmental; D007438:Introns / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D008816:Mice, Jimpy; D008969:Molecular Sequence Data; D018991:Myelin Proteolipid Protein / Q000235:genetics; D009186:Myelin Sheath / Q000502:physiology*; D009419:Nerve Tissue Proteins; D009836:Oligodendroglia / Q000378:metabolism; D017686:Oncorhynchus mykiss; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation; D014982:Xenopus laevis
chemical_list: D001059:Apoproteins; D004274:DNA, Recombinant; D029941:Fish Proteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C489072:PLP protein, Oncorhynchus mykiss; C047516:Plp1 protein, mouse; C489071:Plp1 protein, rat; D012333:RNA, Messenger