Flupirtine ameliorates ischaemic-like death of rat retinal ganglion cells by preventing calcium influx.
The effect of flupirtine on the loss of retinal ganglion cells following transient elevation of intraocular pressure (experimental ischaemia) or NMDA-induced excitotoxicity was studied. Ischaemia (60 min) or intravitreal injection of NMDA (20 nmol) caused a decrease in Thy-1 mRNA and Thy-1 immunoreactivity which are associated with ganglion cells. Administration of flupirtine counteracted these changes. Moreover, flupirtine dose-dependently inhibited NMDA-induced 45Ca(2+) influx into cultured cortical neurones and retinal pieces in vitro with maximal inhibition being observed at 200 microM. A similar concentration of flupirtine failed to inhibit kainate-stimulated calcium influx into cultured cortical neurones. In addition, flupirtine had no significant effect on [3H]nitrendipine or [3H]diltiazem binding to cortical membranes. The present studies are consistent with previous findings which suggested flupirtine to act as a NMDA antagonist by a mechanism that still remains to be clarified.
Nash
M S
MS
Nuffield Laboratory of Ophthalmology, University of Oxford, Walton Street, Oxford, UK.
Wood
J P
JP
Melena
J
J
Osborne
N N
NN
D000631:Aminopyridines / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology; D005333:Fetus; D007511:Ischemia / Q000503:physiopathology*; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D012171:Retinal Vessels / Q000502:physiology / Q000503:physiopathology*; D018800:Thy-1 Antigens / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D000631:Aminopyridines; D018696:Neuroprotective Agents; D012333:RNA, Messenger; D018800:Thy-1 Antigens; D016202:N-Methylaspartate; C034161:flupirtine; D002118:Calcium