Expanded-bed chromatography in primary protein purification.
Chromatography in stable expanded beds enables proteins to be recovered directly from cultivations of microorganisms or cells and preparations of disrupted cells, without the need for prior removal of suspended solids. The general performance of an expanded bed is comparable to a packed bed owing to reduced mixing of the adsorbent particles in the column. However, optimal operating conditions are more restricted than in a packed bed due to the dependence of bed expansion on the size and density of the adsorbent particles as well as the viscosity and density of the feedstock. The feedstock composition may become the most limiting restriction owing to interactions of adsorbent particles with cell surfaces, DNA and other substances, leading to their aggregation and consequently to bed instabilities and channeling. Despite these difficulties, expanded-bed chromatography has found widespread applications in the large scale purification of proteins from mammalian cell and microbial feedstocks in industrial bioprocessing. The basics and implementation of expanded-bed chromatography, its advantages as well as problems encountered in the use of this technique for the direct extraction of proteins from unclarified feedstocks are addressed.
Anspach
F B
FB
Gesellschaft fuer Biotechnologische Forschung mbH, Department of Process Development, Braunschweig, Germany. anspach@gbf.de
Curbelo
D
D
Hartmann
R
R
Garke
G
G
Deckwer
W D
WD
D000327:Adsorption; D000821:Animal Feed / Q000032:analysis; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins