Cloning of two new cry genes from Bacillus thuringiensis subsp. wuhanensis strain.
With PCR products as probes, we have cloned two new cry-type genes from Bacillus thuringiensis subsp. wuhanensis. The deduced amino acid sequence of the first clone is 77.3% identical to Cry1Ga1. The deduced protein sequence of the second clone is 69.8-78.7% identical to that of Cry1B group. The nomenclature assignment of these two clones is, therefore, named Cry1Gb1 and Cry1Bd1, respectively. The Cry1Bd1 is toxic to Plutella xylostella larvae, and the Cry1Gb1 is toxic to Pieris rapae larvae.
Kuo
W S
WS
Institute of Biochemistry, National Yang Ming University, Shih-Pai, Taipei, Taiwan 11221, Republic of China.
Lin
J H
JH
Tzeng
C C
CC
Kao
S S
SS
Chak
K F
KF
D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000633:toxicity; D001427:Bacterial Toxins; D001483:Base Sequence; D002080:Butterflies / Q000187:drug effects; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014118:Toxins, Biological / Q000235:genetics* / Q000633:toxicity
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C406926:CRY1BD protein, Bacillus thuringiensis; C406925:CRY1GB protein, Bacillus thuringiensis; D014118:Toxins, Biological