Activation of caspase-3 in molt4 cells by apoptosis-inducing nucleosides from CD57(+)HLA-DR(bright) natural suppressor cell line.
Apoptosis-inducing nucleosides (AINs), which were released and isolated from CD57(+)HLA-DR(bright) natural suppressor (57.DR-NS) cell line derived from human decidual tissue, induced apoptosis in Molt4 cells. The addition of caspase-3 inhibitor into the reaction blocked the cleavage of caspase-3 and apoptosis in Molt4 cells treated with AINs, detected by flow cytometrical or spectrofluorometrical analysis and DNA fragmentation assay. Furthermore, by means of immunoblotting, the processing of caspase-3 was shown with the appearance of their catalytically active subunits of 20 and 11 kDa during the generation of apoptosis in Molt4 cells treated with AINs. This processing of caspase-3 into active subunits was also blocked by the addition of caspase-3 inhibitor. Thus, it was definitely revealed that the activation of caspase-3 was a key feature in the caspase cascade of AINs-induced apoptosis in Molt4 cells.
Li
X
X
Department of Immunology, University of Tokyo, Tokyo, 108-0071, Japan.
Guo
M
M
Mori
E
E
Mori
T
T
D017209:Apoptosis; D018959:CD57 Antigens / Q000276:immunology; D053148:Caspase 3; D061945:Caspase Inhibitors; D020169:Caspases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004789:Enzyme Activation; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D009705:Nucleosides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D018959:CD57 Antigens; D061945:Caspase Inhibitors; D006684:HLA-DR Antigens; D009705:Nucleosides; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases