Cloning and characterization of a novel human gene encoding a zinc finger protein with 25 fingers.
This study reports cloning and characterization of a human cDNA encoding a novel human zinc finger protein, ZFD25. ZFD25 cDNA is 6118 bp long and has an open reading frame of 2352 bp that encodes a 783 amino acid protein with 25 C2H2-type zinc fingers. The ZFD25 cDNA also contains a region with high sequence similarity to the Krüppel-associated box A and B domain in the 5'-untranslated region, suggesting that ZFD25 belongs to the Krüppel-associated box zinc finger protein family. The ZFD25 gene was localized to chromosome 7q11.2. Northern blot analysis showed that ZFD25 was expressed in a wide range of human organs. In cultured endothelial cells, the mRNA level was decreased upon serum starvation.
Li
X A
XA
National Cardiovascular Center, Suita, Osaka, Japan.
Kokame
K
K
Okubo
K
K
Shimokado
K
K
Tsukamoto
Y
Y
Miyata
T
T
Kato
H
H
Yutani
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C404507:ZNF107 protein, human