title: Determination of dihydroergotamine in human plasma by high-performance liquid chromatography with fluorescence detection.
abstract: Dihydroergotamine, a 5-hydroxytryptamine antagonist, is used for the treatment of vascular headaches. A high-performance liquid chromatography assay with fluorescence detection is described for the determination of dihydroergotamine in plasma. The assay was validated over the concentration range 0.1-10 ng/ml plasma and applied to the analysis of plasma samples from subjects treated intramuscularly and intranasally with 2 mg of dihydroergotamine.
authors.affiliation: Shire Laboratories Inc., Rockville, MD 20850, USA.
authors.forename: M
authors.initials: M
authors.lastname: Murday
authors.forename: A M
authors.initials: AM
authors.lastname: McLean
authors.forename: E
authors.initials: E
authors.lastname: Slaughter
authors.forename: R A
authors.initials: RA
authors.lastname: Couch
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004087:Dihydroergotamine / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D004087:Dihydroergotamine