Visualization and quantitation of cyclooxygenase-1 and -2 activity by digital fluorescence microscopy.
Ornberg
R L
RL
Analytical Sciences Center, Monsanto Company, St. Louis, MO 63198, USA.
Koki
A T
AT
D016475:3T3 Cells; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D016895:Culture Media, Serum-Free; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D003593:Cytoplasm / Q000201:enzymology; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D008565:Membrane Proteins; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology
D016895:Culture Media, Serum-Free; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007527:Isoenzymes; D008565:Membrane Proteins; D016718:Arachidonic Acid; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496512:Ptgs1 protein, mouse