title: Effect of genistein on the temporal coordination of cleavage and compaction in mouse preimplantation embryos.
abstract: Initially, we investigated the effect of genistein, an inhibitor of protein tyrosine kinases, on compaction of the mouse embryo since tyrosine phosphorylation of the cadherin-catenins complex was suggested to down-regulate its adhesive function. Genistein prevented cleavage from the 2- to the 4-cell stage in a concentration-dependent manner. The next cleavage is inhibited at all concentrations used. Time course of intercellular flattening is however identical for both control 8-cell embryos and 4-cell arrested embryos. This confirms that compaction takes place according to a biological clock that does not depend on completion of the third cell cycle. Our results also suggest that, since, in contrast to genistein, protein kinases C modulators are known to cause a premature compaction, diacylglycerol-dependent kinases but not protein tyrosine kinases might be upregulators of compaction.
authors.affiliation: Service de Biologie et Embryologie, Faculté de Médecine et de Pharmacie, Université de Mons-Hainaut, Mons, Belgium.
authors.forename: J J
authors.initials: JJ
authors.lastname: Goval
authors.forename: H
authors.initials: H
authors.lastname: Alexandre
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001683:Biological Clocks; D001756:Blastoderm / Q000187:drug effects*; D015820:Cadherins / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D009024:Morphogenesis / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D015534:Trans-Activators; D051176:beta Catenin
chemical_list: D000199:Actins; C495267:CTNNB1 protein, mouse; D015820:Cadherins; D003598:Cytoskeletal Proteins; D004791:Enzyme Inhibitors; D015534:Trans-Activators; D051176:beta Catenin; D019833:Genistein; D011505:Protein-Tyrosine Kinases