Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000.
The complete 42180-bp nucleotide sequence of the mobilization plasmid pNZ4000, coding for exopolysaccharide (EPS) production in Lactococcus lactis, was determined. This plasmid contains a region involved in EPS biosynthesis, four functional replicons, a region containing mobilization genes, and three origin of transfer (oriT) sequences. Sequences identical to these oriT sequences were also found on two other lactococcal plasmids and a plasmid from Lactobacillus helveticus. Several complete and partial IS elements were identified on pNZ4000, including iso-ISS1, iso-IS946, and iso-IS982 sequences. Furthermore, pNZ4000 contains a gene cluster that may encode a cobalt transport system and a gene that encodes a CorA homologue which may function as a magnesium transporter.
van Kranenburg
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Microbial Ingredients Section, NIZO food research, Kernhemseweg 2, ZB Ede, 6718, The Netherlands. kranenbu@nizo.nl
Kleerebezem
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de Vos
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D027682:Cation Transport Proteins; D002413:Cations, Divalent; D003035:Cobalt / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics
D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D002413:Cations, Divalent; C072716:CorA protein, Salmonella; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C048817:RepB protein, Bacteria; D015534:Trans-Activators; D003035:Cobalt; D008274:Magnesium