Heparin-carrying polystyrene to mediate cellular attachment and growth via interaction with growth factors.
Various sugar-carrying polystyrenes (PSs), which consist of synthetic styrene and sugar moieties, are glycoconjugates that are able to attach to polymeric surfaces. Heparin-carrying PS (HCPS) is especially able to retain the binding of heparin-binding growth factors (GFs) such as vascular endothelial GF 165 (VEGF(165)) or fibroblast GF 2 (FGF-2). Human skin fibroblast cells, human coronary smooth muscle cells, and human coronary endothelial cells have good adherence to the HCPS-coated plate. The growth rate of fibroblast cells on HCPS-coated plates is higher than or comparable to fibronectin-coated, gelatin-coated, or tissue culture treated plates, and the HCPS coating inhibits the growth of smooth muscle cells. On the other hand, the growth rate of endothelial cells on HCPS-coated plates in the presence of either VEGF(165) or FGF-2 is comparable to that on fibronectin-coated, gelatin-coated, and tissue culture treated plates. Endothelial cells grow at a higher rate on HCPS-coated plates retained with either VEGF(165) or FGF-2 than on the other coated plates. These results indicate that growth of various cells can be controlled by the HCPS coating, thereby retaining the bioactivity of molecules such as heparin-binding GFs. Thus, HCPS-coated surfaces control selective growth of various cells.
Ishihara
M
M
National Defense Medical College, Research Institute, Division of Biomedical Engineering, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Saito
Y
Y
Yura
H
H
Ono
K
K
Ishikawa
K
K
Hattori
H
H
Akaike
T
T
Kurita
A
A
D001672:Biocompatible Materials; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006493:Heparin; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011137:Polystyrenes
D001672:Biocompatible Materials; D011137:Polystyrenes; D006493:Heparin