title: Heterogeneous behavior of anti-beta2-glycoprotein I antibodies on various commercially available enzyme immunoassay plates coated with beta2-glycoprotein I.
abstract: OBJECTIVE
  To evaluate commercially available enzyme immunoassay (EIA) plates for the measurement of anti-beta2-glycoprotein I autoantibody (anti-beta2-GPI).
  
  
  METHODS
  Sera from 10 patients with the antiphospholipid syndrome, and 3 monoclonal anti-beta2-GPI antibodies established from patients with antiphospholipid syndrome, were assayed for binding to solid phase beta2-GPI on 20 commercially available plates.
  
  
  RESULTS
  Several commercially available EIA plates were found to be of potential value for the measurement of anti-beta2-GPI autoantibody. Some plates were unsuitable for anti-beta2-GPI detection, possibly due to less beta2-GPI on the plates, or to differences in the nature of conformational changes of beta2-GPI induced by the plates.
  
  
  CONCLUSIONS
  Differences among EIA plates need to be considered when measuring beta2-GPI antibodies.
authors.affiliation: Department of Medicine II, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tsutsumi
authors.forename: K
authors.initials: K
authors.lastname: Ichikawa
authors.forename: E
authors.initials: E
authors.lastname: Matsuura
authors.forename: K I
authors.initials: KI
authors.lastname: Sawada
authors.forename: T
authors.initials: T
authors.lastname: Koike
mesh_terms: D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I
chemical_list: D001323:Autoantibodies; D006023:Glycoproteins; D053482:beta 2-Glycoprotein I