title: Immunogenicity of in vitro folded outer membrane protein PorA of Neisseria meningitidis.
abstract: In vitro folded and the denatured form of PorA P1.6 from Neisseria meningitidis strain M990 were used for immunization studies in mice. Previously, the antigen was isolated from cytoplasmic inclusion bodies, folded and purified. Its immunogenicity without adjuvant appeared to be low. The addition of the adjuvant QuilA, but not of galE lipooligosaccharide, considerably enhanced the immunogenicity. Moreover, when immunized with folded PorA P1.6 plus QuilA, a clear switch towards the IgG2a subclass of antibodies and concomitantly, the appearance of serum bactericidal activity, which is believed to be important for protective immunity, was observed. Hence, a tool for preparing vaccines against serogroup B meningococci devoid of endotoxin is available.
authors.affiliation: Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Jansen
authors.forename: B
authors.initials: B
authors.lastname: Kuipers
authors.forename: J
authors.initials: J
authors.lastname: van der Biezen
authors.forename: H
authors.initials: H
authors.lastname: de Cock
authors.forename: P
authors.initials: P
authors.lastname: van der Ley
authors.forename: J
authors.initials: J
authors.lastname: Tommassen
mesh_terms: D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001428:Bacterial Vaccines / Q000276:immunology*; D001770:Blood Bactericidal Activity; D004797:Enzyme-Linked Immunosorbent Assay; D007114:Immunization; D008585:Meningitis, Meningococcal / Q000517:prevention & control; D022401:Meningococcal Vaccines; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000276:immunology* / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D018272:Porins / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D017510:Protein Folding; D062605:Quillaja Saponins; D012503:Saponins / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D022401:Meningococcal Vaccines; D009842:Oligopeptides; D018272:Porins; D062605:Quillaja Saponins; D012503:Saponins; C057699:porin protein, Neisseria; C046386:Quil A