Dynein light chain binding to a 3'-untranslated sequence mediates parathyroid hormone mRNA association with microtubules.
The 3'-untranslated region (UTR) of mRNAs binds proteins that determine mRNA stability and localization. The 3'-UTR of parathyroid hormone (PTH) mRNA specifically binds cytoplasmic proteins. We screened an expression library for proteins that bind the PTH mRNA 3'-UTR, and the sequence of 1 clone was identical to that of the dynein light chain LC8, a component of the dynein complexes that translocate cytoplasmic components along microtubules. Recombinant LC8 binds PTH mRNA 3'-UTR, as shown by RNA electrophoretic mobility shift assay. We showed that PTH mRNA colocalizes with microtubules in the parathyroid gland, as well as with a purified microtubule preparation from calf brain, and that this association was mediated by LC8. To our knowledge, this is the first report of a dynein complex protein binding an mRNA. The dynein complex may be the motor that is responsible for transporting mRNAs to specific locations in the cytoplasm and for the consequent is asymmetric distribution of translated proteins in the cell.
Epstein
E
E
Minerva Center for Calcium and Bone Metabolism, Nephrology Services, Hadassah University Hospital, Jerusalem, Israel 91120.
Sela-Brown
A
A
Ringel
I
I
Kilav
R
R
King
S M
SM
Benashski
S E
SE
Yisraeli
J K
JK
Silver
J
J
Naveh-Many
T
T
D020413:3' Untranslated Regions / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002451:Cell Compartmentation; D004398:Dyneins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D020409:Molecular Motor Proteins; D010281:Parathyroid Hormone / Q000235:genetics*; D011485:Protein Binding; D020871:RNA Stability; D051381:Rats
D020413:3' Untranslated Regions; D020409:Molecular Motor Proteins; D010281:Parathyroid Hormone; D004398:Dyneins