Determination of flunarizine in rat brain by liquid chromatography-electrospray mass spectrometry.
A rapid liquid chromatography-electrospray mass spectrometry (LC-ES-MS) assay for the determination of flunarizine (FZ) in rat brain has been developed. A C18 column and an isocratic elution were employed for the separation. Using post-column split, 64% of the eluent was introduced into the ES-MS system for detection. The [M+H]+ (m/z 406) and a fragmented ion (m/z 203) were detected using selected ion monitoring. The linear range of this assay was good, ranging from 0.05 to 5 microM (r2=0.99). The intra- and inter-day precisions showed relative standard deviations ranging from 1.4% to 2.0% and 1.3% to 2.9%, respectively. The application of this newly developed method was demonstrated by examining the pharmacokinetics of FZ in rat brain.
Fuh
M R
MR
Department of Chemistry, Soochow University, Taipei, Taiwan. msfuh@mail.scu.edu.tw
Hsieh
C J
CJ
D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002121:Calcium Channel Blockers / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D005444:Flunarizine / Q000032:analysis* / Q000493:pharmacokinetics; D006634:Histamine H1 Antagonists / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D002121:Calcium Channel Blockers; D006634:Histamine H1 Antagonists; D005444:Flunarizine