Requirements for alternative forms of the activator domain, P5abc, in the Tetrahymena ribozyme.
The role of P5abc domain of the Tetrahymena LSU self-splicing Group I intron is to enhance the activity of the intron via tertiary interactions involving A-rich bulge and terminal loops L5b and L5c. We constructed and examined alternative forms of the domain that accelerate the ribozymatic reaction. The results indicate that the characteristic structure of P5c subdomain plays an important role by forming L2xL5c interaction (P14) and that the region flanking P5c subdomain can be significantly mutable without much affecting the activity of the ribozyme.
Naito
Y
Y
Graduate School of Biostudies, Kyoto University, Japan.
Shiraishi
H
H
Inoue
T
T
D000818:Animals; D001483:Base Sequence; D007438:Introns; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D016337:RNA, Catalytic / Q000737:chemistry* / Q000235:genetics; D013768:Tetrahymena / Q000235:genetics*
D016337:RNA, Catalytic