title: Differential expressions of protein kinase C isozymes during proliferation and differentiation of human skeletal muscle cells in vitro.
abstract: The mechanism of skeletal muscle regeneration in vivo can be well modeled in vitro by culturing skeletal muscle cells. In these cultures mononuclear satellite cells fuse to form polynuclear myotubes by proliferation and differentiation. The aim of this study was to determine how the different protein kinase C (PKC) isozymes were expressed during differentiation of human skeletal muscle in vitro. The expressions of desmin, used as a muscle-specific intermediate filament protein marker of differentiation, and of different PKC isozymes were detected by single and double immunohistochemical labeling, and by Western blot analysis. In skeletal muscle cells we could identify five PKC isozymes (PKC alpha, -gamma, -etha, -theta and -zeta). The expressions of PKC alpha and -zeta did not change significantly during differentiation; their levels of expression were high in the early immature cells and remained unchanged in later phases. In contrast, the expression levels of PKC gamma and -etha increased with differentiation. Furthermore, the cellular localization of PKC gamma markedly altered during differentiation, with a perinuclear-nuclear to cytoplasmic translocation. The change in the level of expression of PKC theta during differentiation showed different pattern; its expression was high during the early phases, but a decreased immunostaining was detected in the matured, well-differentiated myotubes. We conclude, therefore, that cultured human skeletal muscle cells possess a characteristic PKC isozyme pattern, and that the different phases of differentiation are accompanied by different expression patterns of the various isozymes. These data suggest the possible functional and differential roles of PKC isozymes in human skeletal muscle differentiation.
authors.affiliation: Department of Physiology and Cell Physiology, University Medical School of Debrecen, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Boczán
authors.forename: S
authors.initials: S
authors.lastname: Boros
authors.forename: F
authors.initials: F
authors.lastname: Mechler
authors.forename: L
authors.initials: L
authors.lastname: Kovács
authors.forename: T
authors.initials: T
authors.lastname: Bíró
mesh_terms: D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis*; D018482:Muscle, Skeletal / Q000166:cytology* / Q000201:enzymology*; D011493:Protein Kinase C / Q000032:analysis / Q000096:biosynthesis*; D051571:Protein Kinase C-alpha; D000074121:Protein Kinase C-theta; D013997:Time Factors; D000099221:Protein Kinase C zeta
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D000074121:Protein Kinase C-theta; D000099221:Protein Kinase C zeta; C087857:protein kinase C gamma; C119190:protein kinase C nu; C496628:PRKCA protein, human; C076233:PRKCQ protein, human