[Effects of low temperature storage on DNA migration assayed by single cell gel electrophoresis].
V79 cells treated with potassium dichromate and control cells were stored at 4 degrees C, -20 degrees C, -80 degrees C and -196 degrees C. Single cell gel electrophoresis (SCGE) was carried out after preserving cells for 0, 4, 24, 48 and 168 hours. The results showed that there was no difference on DNA migration between cells processing immediately after trypsinization and cells preserving for 24 h at different low temperatures. Longer storage (48 h or 168 h) resulted in a significant increase in the length of DNA migration and decrease in cell viability. The results suggested that 4 degrees C is more convenient for less than 24 h storage without influence on DNA migration and -80 degrees C is better for longer storage to minimize the influence on DNA migration.
Zhang
Z
Z
Department of Environmental Health, School of Public Health, West China University of Medical Sciences, Chengdu, China.
Li
R
R
Tan
Z
Z
Heng
Z
Z
D000818:Animals; D002460:Cell Line; D020552:Comet Assay; D006224:Cricetinae; D015925:Cryopreservation; D004249:DNA Damage; D005347:Fibroblasts / Q000166:cytology; D008168:Lung / Q000166:cytology; D011192:Potassium Dichromate; D013696:Temperature
D011192:Potassium Dichromate