title: Decreased lipid fluidity of the erythrocyte membrane in dogs with leishmaniasis-associated anaemia.
abstract: In both man and the dog, anaemia resulting from natural leishmaniasis is often severe and mainly associated with a shortened life span of erythrocytes. Lipid fluidity of erythrocyte membranes from 17 dogs with anaemia caused by visceral leishmaniasis was investigated by means of fluorescence polarization. Results were compared with those from three groups of control animals (10 healthy dogs, seven dogs with visceral leishmaniasis but no anaemia, and 10 dogs with anaemia unrelated to leishmaniasis). Fluorescence polarization values recorded for animals with leishmaniasis-associated anaemia were elevated-indicating reduced erythrocyte membrane fluidity-and significantly higher than in the control groups. Mechanical sequestration by the spleen due to increased cell rigidity, or alterations in receptor/ligand erythrocyte cytoadherence mechanisms, or both, may result from decreased membrane fluidity and hence contribute to the anaemia of Leishmania -infected dogs.
authors.affiliation: Dipartimento di Scienze Cliniche Veterinarie, Sezione di Clinica Medica, Università Federico II, via Federico Delpino 1, Naples, 80137, Italy.
authors.forename: R
authors.initials: R
authors.lastname: De Luna
authors.forename: M
authors.initials: M
authors.lastname: Ferrante
authors.forename: L
authors.initials: L
authors.lastname: Severino
authors.forename: R
authors.initials: R
authors.lastname: Ambrosio
authors.forename: D
authors.initials: D
authors.lastname: Piantedosi
authors.forename: L
authors.initials: L
authors.lastname: Gradoni
authors.forename: A
authors.initials: A
authors.lastname: Lucisano
authors.forename: A
authors.initials: A
authors.lastname: Persechino
mesh_terms: D000740:Anemia / Q000469:parasitology / Q000662:veterinary*; D000818:Animals; D004283:Dog Diseases / Q000097:blood / Q000469:parasitology*; D004285:Dogs; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005454:Fluorescence Polarization; D007898:Leishmaniasis, Visceral / Q000150:complications*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism
chemical_list: D008563:Membrane Lipids