Time-resolved detection of singlet oxygen luminescence in red-cell ghost suspensions: concerning a signal component that can be attributed to 1O2 luminescence from the inside of a native membrane.
For about ten years, it has been debated whether in principle it is possible to detect 1O2 located within the cell membrane by performing experiments with cell suspensions or even in tissue. In this paper we present our investigations on photosensitized red-cell ghost suspensions (RCGSs) and our strategy for the detection of luminescence of singlet oxygen (1O2) from the inside of the cell membrane. Using a very sensitive apparatus for time-resolved 1O2 detection, a very promising sensitizer and an adequate experimental strategy, a very small amount of the detected luminescence indeed can be attributed to 1O2 from the inside of the ghost membrane.
Oelckers
S
S
Humboldt Universität zu Berlin, Institut für Physik, AG Photobiophysik, Germany.
Ziegler
T
T
Michler
I
I
Röder
B
B
D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008163:Luminescent Measurements; D010100:Oxygen / Q000032:analysis*; D013997:Time Factors
D010100:Oxygen