Screening of selected plant extracts for in vitro inhibitory activity on HIV-1 reverse transcriptase (HIV-1 RT).
Methanolic-aqueous extracts of 70 plants were investigated for their ability to inhibit HIV-1 reverse transcriptase activity in vitro. Two thirds of the extracts screened showed more than 50% inhibition. Two extracts inhibited the enzyme completely while four exhibited more than 90% inhibition. Tannins as nonspecific HIV-1 RT inhibitors were detected and removed from the extracts. The IC50 values of the most potent extracts after the removal of tannins for the HIV-1 RT inhibition are as follows: Sambucus racemosa 0.017 mg/ml and Geranium phaeum 0.067 mg/ml. Daunomycine was chosen as a standard substance in the non-radioactive immuno assay used for screening. As a result from the future isolation and characterization of these compounds, new leading structures are expectable.
Mlinaric
A
A
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Slovenia.
Kreft
S
S
Umek
A
A
Strukelj
B
B
D004797:Enzyme-Linked Immunosorbent Assay; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D013634:Tannins / Q000737:chemistry / Q000494:pharmacology
D010936:Plant Extracts; D018894:Reverse Transcriptase Inhibitors; D013634:Tannins; D054303:HIV Reverse Transcriptase