title: Syncollin is differentially expressed in rat proximal small intestine and regulated by feeding behavior.
abstract: Gradients of gene expression are maintained along the proximal-distal axis of the mammalian small intestine despite a continuously regenerating epithelium. To study the molecular mechanisms responsible for this phenomenon, we utilized a subtractive hybridization strategy to isolate genes differentially expressed in the duodenum but not ileum. We isolated and sequenced 15 clones. The clones were fragments of genes encoding lipases, proteases, and an esterase. A novel clone was characterized and subsequently shown to encode syncollin, a secretory granule protein that binds to syntaxin in a calcium-sensitive manner. RT-PCR and S1 nuclease protection assay were used to clarify the 5'-end of syncollin. Syncollin was expressed in the rat pancreas, spleen, duodenum, and colon. In situ hybridization localized syncollin expression in the pancreas to acinar cells and in the duodenum to villus epithelial cells.
authors.affiliation: Department of Physiology, Faculty of Medicine, National University of Singapore, Singapore 119260.
authors.forename: S
authors.initials: S
authors.lastname: Tan
authors.forename: S C
authors.initials: SC
authors.lastname: Hooi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D004386:Duodenum / Q000378:metabolism; D005215:Fasting; D005502:Food; D005786:Gene Expression Regulation; D007082:Ileum / Q000378:metabolism; D017403:In Situ Hybridization; D007421:Intestine, Small / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D012333:RNA, Messenger; C107454:Sycn protein, rat; D015719:Single-Strand Specific DNA and RNA Endonucleases