Enantiomeric synthesis of (S)-2-methylbutanoic acid methyl ester, apple flavor, using lipases in organic solvent.
Enantiomeric selective synthesis of (S)-2-methylbutanoic acid methyl ester, which is known as a major apple and strawberry flavor, was performed from racemic 2-methylbutanoic acid using lipases in organic solvent. Among 20 lipases, lipase IM 20 (immobilized lipase of Rhizomucor miehei), lipase AP (Aspergillus niger), and lipase FAP-15 (Aspergillus javanicus) exhibited higher enzymatic activities and enantioselectivities and were selected for the synthesis of (S)-2-methylbutanoic acid methyl ester. Using these enzymes, the reaction conditions such as temperature and lyophilizing pH were optimized, and kinetic parameters were determined. All of the reactions were performed in isooctane, which was identified as the best reaction media for nonaqueous systems. At 20 degrees C maximum enantiomeric excess was observed, while synthetic activity increased as the temperature increased. Only lipases lyophilized at pH 5.5, 6. 0, 6.5, and 7.0 showed synthetic activity. In this pH range, enantioselectivities were not influenced by the lyophilizing pH. The K(M,S) and K(M,R) values for ester synthetic activity of lipase were 1120 and 1240 mM, respectively. Enzyme activity was inhibited by (S)-2-methylbutanoic amide, and its K(i) was calculated as 84 mM. (S)-2-Methylbutanoic amide acted as a competitive inhibitor.
Kwon
D Y
DY
Food Science and Biotechnology Division, Korea Food Research Institute, San 46-1, Paekhyondong, Poondang, Songnam, Kyongki-do 463-420, Republic of Korea. dykwon@kfri.re.kr
Hong
Y J
YJ
Yoon
S H
SH
D002087:Butyrates / Q000138:chemical synthesis*; D005421:Flavoring Agents / Q000138:chemical synthesis*; D005638:Fruit / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D012997:Solvents; D013696:Temperature
D002087:Butyrates; D005421:Flavoring Agents; D012997:Solvents; D008049:Lipase; C019475:2-methylbutanoic acid