Optimal conditions for simultaneous measurement of platelet aggregation and ATP secretion in canine whole blood.
This paper describes the optimal conditions for simultaneous evaluation of platelet aggregation and secretion capacity in canine whole blood using a Whole Blood Lumi-Aggregometer (Chrono-Log Corporation, Havertown, Pensylvania). For this purpose, the potential influence of several parameters was investigated using collagen, adenosinediphosphate (ADP), arachidonic acid (AA) and thrombin as platelet agonists. Results indicate that optimal experimental conditions to obtain reliable results include: allowing blood samples to stand at room temperature 60 minutes after blood collection, analysing samples within 3 hours from time of collection, adjusting platelet numbers to a final concentration of 150 000 microl(-1)and mixing the sample with isotonic saline (1:1) before adding the platelet agonist. The use of different platelet agonists offers variable results: collagen (0.5, 1 and 5 microg ml(-1)) is suitable for simultaneous platelet aggregation and adenosintriphosphate (ATP) secretion measurements; 1 UI ml(-1)of thrombin induced maximum ATP secretion;AA (0.5 and 1 mM) and ADP (5, 10 and 25 microM) did not give consistent results. The method described in this study has important clinical applications since it allows easy and quick platelet function evaluation in pathologic states.
Jüttner
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Departamento de Patología Animal II, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Rodríguez
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Fragío
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D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood*; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary; D003094:Collagen / Q000494:pharmacology; D004285:Dogs / Q000097:blood*; D005260:Female; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D015203:Reproducibility of Results; D013917:Thrombin / Q000494:pharmacology
D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D003094:Collagen; D013917:Thrombin