[Relationship between the expression level of alpha-MHC gene and cardiac contractility during heart failure].
To investigate the molecular basis of the decrease in myocardial contractility during heart failure, an animan model of heart failure was set up by means of deoxycorticosterone-acetate impregnated silicone rubber implants in wistar rats. Cardiac contractility in normal and heart failure rats was examined, and gene expression of its myocardial contractile protein, alpha-MHC, was quantitatively analyzed at gene transcription level by using RNA slot blot hybridization. The results showed that the cardiac contractility and the alpha-MHC mRNA levels in heart failure rats were all lower than those in the normal. Statistical analysis showed a positive correlation between the cardiac contractility and the gene expression of alpha-MHC (r = 0.4143, n = 43, P < 0.05). The above results indicate that gene expression level of alpha-MHC is one of the key factors determining cardiac contractility.
Wei
L
L
Wang
L
L
Ouyang
X
X
Ying
H
H
Liu
S
S
Qu
Y
Y
Liu
B
B
Wang
Z
Z
D000818:Animals; D002303:Cardiac Output, Low / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D015870:Gene Expression; D008297:Male; D009200:Myocardial Contraction; D018995:Myosin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D018995:Myosin Heavy Chains