title: Confirmation of eprinomectin, moxidectin, abamectin, doramectin, and ivermectin in beef liver by liquid chromatography/positive ion atmospheric pressure chemical ionization mass spectrometry.
abstract: A liquid chromatographic (LC) multiresidue screening procedure was developed for determination of eprinomectin, moxidectin, abamectin, doramectin, and ivermectin in beef liver at 0, 25, 50, and 100 ppb levels. A procedure using low resolution LC/atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) was developed with further purification steps added to the quantitative LC method to confirm residues. Acetonitrile extracts of liver, prior to derivatization for LC analysis, were further purified by using a C8 solid-phase extraction cartridge and an alumina-B cartridge. The purified extract was analyzed by injection into an LC/positive ion APCI MS. Identity of the compound was confirmed by comparison of its retention time and relative intensity data with those of a standard or recovery from a fortified control liver sample. Anthelmintic drugs in acetonitrile extracts of liver containing eprinomectin, moxidectin, abamectin, doramectin, and ivermectin at 25 ppb, the lowest level of fortification used in the LC determinative method, were successfully confirmed.
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, Atlanta, GA 30604, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Ali
authors.forename: T
authors.initials: T
authors.lastname: Sun
authors.forename: G E
authors.initials: GE
authors.lastname: McLeroy
authors.forename: E T
authors.initials: ET
authors.lastname: Phillippo
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis; D000969:Antinematodal Agents / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D018942:Macrolides; D013058:Mass Spectrometry / Q000379:methods; D011786:Quality Control
chemical_list: D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D000969:Antinematodal Agents; D018942:Macrolides; C048324:abamectin; D007559:Ivermectin; C101434:eprinomectin; C084101:doramectin; C027837:moxidectin