Reconstruction of human hard-palate mucosal epithelium on de-epidermized dermis.
Artificial hard-palate mucosa equivalents were reconstructed using keratinocytes derived from normal human hard-palate and de-epidermized dermis. Reconstructed hard-palate mucosal epithelium formed in three-dimensional culture was compared to native hard-palate mucosal epithelium and reconstructed oral buccal mucosal epithelium with regard to keratin expression. Artificial hard-palate mucosal epithelium reconstructed in medium with delipidized serum showed a differentiation pattern similar to that of hard-palate epithelium in vivo. The present study also confirmed that keratinocytes derived from hard-palate mucosa are intrinsically different from those of nonkeratinizing oral surfaces.
Cho
K H
KH
Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, South Korea. khcho@snu.ac.kr
Ahn
H T
HT
Park
K C
KC
Chung
J H
JH
Kim
S W
SW
Sung
M W
MW
Kim
K H
KH
Chung
P H
PH
Eun
H C
HC
Youn
J I
JI
D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D004817:Epidermis; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology*; D010159:Palate / Q000166:cytology*