title: Synthesis of dolichyl phosphate derivatives with fluorescent label at the omega-end of the chain, new tools to study protein glycosylation.
abstract: Derivatives of dolichyl phosphate (Dol-P) with 2-aminopyridine or 1-aminonaphtalene fluorophore groups at the omega-end of the chain were synthesized. These products serve as substrates for recombinant yeast Dol-P-mannose synthase. Fluorescence resonance energy transfer between a Trp residue of the enzyme and the 1-aminonaphtalene group of the Dol-P analogue was demonstrated.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow. shiba@ioc.ac.ru
authors.forename: V N
authors.initials: VN
authors.lastname: Shibaev
authors.forename: V V
authors.initials: VV
authors.lastname: Veselovsky
authors.forename: A V
authors.initials: AV
authors.lastname: Lozanova
authors.forename: S D
authors.initials: SD
authors.lastname: Maltsev
authors.forename: L L
authors.initials: LL
authors.lastname: Danilov
authors.forename: W T
authors.initials: WT
authors.lastname: Forsee
authors.forename: J
authors.initials: J
authors.lastname: Xing
authors.forename: H C
authors.initials: HC
authors.lastname: Cheung
authors.forename: M J
authors.initials: MJ
authors.lastname: Jedrzejas
mesh_terms: D015057:1-Naphthylamine / Q000737:chemistry; D000631:Aminopyridines / Q000737:chemistry; D004288:Dolichol Phosphates / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006023:Glycoproteins / Q000737:chemistry*; D007700:Kinetics; D008364:Mannosyltransferases / Q000378:metabolism; D013379:Substrate Specificity; D015003:Yeasts / Q000201:enzymology
chemical_list: D000631:Aminopyridines; D004288:Dolichol Phosphates; D005456:Fluorescent Dyes; D006023:Glycoproteins; D015057:1-Naphthylamine; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase; C032439:alpha-aminopyridine