Reactive oxidant species in piriform cortex extracellular fluid during seizures induced by systemic kainic acid in rats.
Kainic acid (KA) administered systemically to rats produces seizures and brain damage. We measured an increase in reactive oxidant species (ROS) during KA-induced seizures in the extracellular fluid (ECF) of the piriform cortex, a brain region known to be subsequently damaged. Intracerebral microdialysis samples were collected and assayed for isoluminol-dependent chemiluminescence before and after injection of KA (16 mg/kg, i.p.). Hydrogen peroxide (H2O2) concentrations were calculated from catalase-sensitive chemiluminescence, the difference between total and catalase-resistant chemiluminescence. During generalized tonic-clonic seizures, both total and catalase-resistant chemiluminescence increased significantly in samples from brain ECF. Catalase-resistant chemiluminescence, most likely produced by ascorbic acid, increased for a full hour during sustained seizure activity. H2O2 concentrations showed a trend towards elevation during seizures. Increased ROS suggest that oxidative stress occurs in brain ECF during sustained seizure activity.
Layton
M E
ME
Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, USA.
Pazdernik
T L
TL
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism
D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D007608:Kainic Acid