title: Biochemical/spectroscopic characterization and preliminary X-ray analysis of a new aldehyde oxidoreductase isolated from Desulfovibrio desulfuricans ATCC 27774.
abstract: Aldehyde oxidoreductase (AOR) activity has been found in different sulfate reducing organisms (Moura, J. J. G., and Barata, B. A. S. (1994) in Methods in Enzymology (Peck, H. D., Jr., and LeGall, J., Eds.), Vol. 243, Chap. 4. Academic Press; Romão, M. J., Knäblein, J., Huber, R., and Moura, J. J. G. (1997) Prog. Biophys. Mol. Biol. 68, 121-144). The enzyme was purified to homogeneity from extracts of Desulfovibrio desulfuricans (Dd) ATCC 27774, a sulfate reducer that can use sulfate or nitrate as terminal respiratory substrates. The protein (AORDd) is described as a homodimer (monomer, circa 100 kDa), contains a Mo-MCD pterin, 2 x [2Fe-2S] clusters, and lacks a flavin group. Visible and EPR spectroscopies indicate a close similarity with the AOR purified from Desulfovibrio gigas (Dg) (Barata, B. A. S., LeGall, J., and Moura, J. J. G. (1993) Biochemistry 32, 11559-11568). Activity and substrate specificity for different aldehydes were determined. EPR studies were performed in native and reduced states of the enzyme and after treatment with ethylene glycol and dithiothreitol. The AORDd was crystallized using ammonium sulfate as precipitant and the crystals belong to the space group P6(1)22, with unit cell dimensions a = b = 156.4 and c = 177.1 A. These crystals diffract to beyond 2.5 A resolution and a full data set was measured on a rotating anode generator. The data were used to solve the structure by Patterson Search methods, using the model of AORDg.
authors.affiliation: Departamento de Química (and Centro de Química Fina e Biotecnologia), Universidade Nova de Lisboa, Monte de Caparica, 2825-114, Portugal.
authors.forename: R O
authors.initials: RO
authors.lastname: Duarte
authors.forename: M
authors.initials: M
authors.lastname: Archer
authors.forename: J M
authors.initials: JM
authors.lastname: Dias
authors.forename: S
authors.initials: S
authors.lastname: Bursakov
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: I
authors.initials: I
authors.lastname: Moura
authors.forename: M J
authors.initials: MJ
authors.lastname: Romão
authors.forename: J J
authors.initials: JJ
authors.lastname: Moura
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D003067:Coenzymes; D018360:Crystallography, X-Ray; D003901:Desulfovibrio / Q000201:enzymology*; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D008667:Metalloproteins / Q000737:chemistry; D008970:Molecular Weight; D000090006:Molybdenum Cofactors; D010084:Oxidation-Reduction; D020836:Protein Structure, Quaternary; D011621:Pteridines / Q000737:chemistry; D013053:Spectrophotometry; D013379:Substrate Specificity
chemical_list: D000445:Aldehyde Oxidoreductases; D003067:Coenzymes; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; C022261:molybdopterin