Expression of insulin-like growth factor-I in rat glioma cells is associated with change in both immunogenicity and apoptosis.
Insulin-like growth factor I (IGF-I), has a role in cellular differentiation and is also expressed in neoplastic transformation of glioma cells. We recently demonstrated inhibition in expression of cellular IGF-I after transfection with vectors that incodes a segment of the human IGF-I RNA in antisense orientation. The transfected cells expressed increased levels of both MHC-I and B7 molecules. In this paper we show that IGF-I antisense transfected cells also become apoptotic. Moreover, the phenomenon of programmed cell death is related to the phenomenon that results in increased expression of MHC-I and B7 molecules. Co-transfection of rat glioma cells with the vector expressing IGF-I antisense RNA and with vectors encoding the expression of MHC-I and B7 antisense cDNA suppressed the expression of both of these molecules and was associated with a decrease in apoptosis.
Ly
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Laboratory of Developmental Neurology, INSERM & University Paris VII, Hôpital Robert Debré, 48 Boulevard Sérurier, 75019, Paris, France.
Bouchaud
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C
Henin
D
D
Sanson
M
M
Delattre
J Y
JY
Pan
Y
Y
Anthony
D
D
Duc
H T
HT
Evrard
P
P
Trojan
J
J
D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology; D018122:B7-1 Antigen / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020287:In Situ Nick-End Labeling; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics*; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D018122:B7-1 Antigen; D015395:Histocompatibility Antigens Class I; D016372:RNA, Antisense; D007334:Insulin-Like Growth Factor I