An investigation into the electro-physical characteristics of microbial cells during the metabolism of toxic compounds under conditions of limited O2 availability.
The purpose of the work reported here was to experimentally clarify the interconnection between changes in the electro-physical characteristics of microbial suspensions and processes of the metabolism of certain toxic compounds (acrylamide and p-nitrophenol (PNP)) in cells containing enzyme systems of the initial metabolism of these compounds. In this work, we used cells of two strains, Acinetobacter calcoaceticum A-122 and Brevibacterium sp. 13PA, which are capable of utilising PNP and acrylamide, respectively, as the sole source of carbon. Suspensions of these cells exhibited appreciable decreases in the magnitude of the electro-optical signal when the microbial metabolism of PNP and acrylamide was inhibited under conditions of limited O2 supply. This attests to a relationship between the electro-physical characteristics of microbial suspensions and cellular metabolic reactions and also to the negligibly small effect that the non-specific interaction of substrate with the cells has on the suspensions' electro-optical properties.
Ignatov
O V
OV
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia. oignatov@ibppm.saratov.su
Khorkina
N A
NA
Shchyogolev SYu
Singirtsev
I N
IN
Bunin
V D
VD
Tumaikina
Y A
YA
Ignatov
V V
VV
D016954:Acinetobacter calcoaceticus / Q000201:enzymology / Q000378:metabolism*; D020106:Acrylamide / Q000378:metabolism*; D001951:Brevibacterium / Q000201:enzymology / Q000378:metabolism*; D003470:Culture Media; D004594:Electrophysiology; D009596:Nitrophenols / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000502:physiology*; D013053:Spectrophotometry
D003470:Culture Media; D009596:Nitrophenols; D020106:Acrylamide; D010100:Oxygen; C024836:4-nitrophenol