title: Localization of the tetrodotoxin-resistant sodium channel NaN in nociceptors.
abstract: Tetrodotoxin-resistant sodium currents contribute to the somal and axonal sodium currents of small diameter primary sensory neurons, many of which are nociceptive. NaN is a recently described tetrodotoxin-resistant sodium channel expressed preferentially in IB4-labeled dorsal root ganglion (DRG) neurons. We employed an antibody raised to a NaN specific peptide to show that NaN is preferentially localized along axons of IB4-positive unmyelinated fibers in the sciatic nerve and in axon terminals in the cornea. NaN immunoreactivity was also found at some nodes of Ranvier of thinly myelinated axons of the sciatic nerve, where it was juxtaposed to Kv1.2 potassium channel immunoreactivity. This distribution of NaN is consistent with a role for NaN sodium channels in nociceptive transmission.
authors.affiliation: Department of Neurology, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: J
authors.initials: J
authors.lastname: Fjell
authors.forename: P
authors.initials: P
authors.lastname: Hjelmström
authors.forename: W
authors.initials: W
authors.lastname: Hormuzdiar
authors.forename: M
authors.initials: M
authors.lastname: Milenkovic
authors.forename: F
authors.initials: F
authors.lastname: Aglieco
authors.forename: L
authors.initials: L
authors.lastname: Tyrrell
authors.forename: S
authors.initials: S
authors.lastname: Dib-Hajj
authors.forename: S G
authors.initials: SG
authors.lastname: Waxman
authors.forename: J A
authors.initials: JA
authors.lastname: Black
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D003315:Cornea / Q000294:innervation; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009186:Myelin Sheath / Q000502:physiology; D062558:NAV1.9 Voltage-Gated Sodium Channel; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000187:drug effects / Q000648:ultrastructure; D009479:Neuropeptides / Q000187:drug effects*; D009619:Nociceptors / Q000187:drug effects*; D017729:Presynaptic Terminals / Q000502:physiology / Q000648:ultrastructure; D011901:Ranvier's Nodes / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology / Q000648:ultrastructure; D015222:Sodium Channels / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D062558:NAV1.9 Voltage-Gated Sodium Channel; D009479:Neuropeptides; C113705:Scn11a protein, rat; D015222:Sodium Channels; D013779:Tetrodotoxin