Detection of DNA fragmentation of myonuclei in myotonic dystrophy by double staining with anti-emerin antibody and by nick end-labeling.
To clarify the occurrence of apoptosis in skeletal muscle in pathological conditions, we studied 44 muscle biopsy specimens by immunohistochemical staining with monoclonal antibody against emerin, which is localized in muscle nuclear membrane, and by ApopTag Plus to detect DNA fragmentation. Five of six patients with myotonic dystrophy (DM) showed three to 35 myonuclei stained with anti-emerin antibody and ApopTag Plus in 1500 muscle fibers. Four of the 18 patients with polymyositis, one of those with thyroid myopathy and one with neurogenic atrophy showed a few myonuclei stained positively by these methods. Our study revealed that DNA fragmentation in myonuclei occurred in skeletal muscle fibers regardless of the type of disease, although the frequency was rather low in all of these diseases except DM. The DNA fragmentation detected in most of the patients with DM suggested a significant role of apoptosis in the pathomechanism of this disease.
Yamada
H
H
Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Japan.
Nakagawa
M
M
Higuchi
I
I
Horikiri
T
T
Osame
M
M
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D017209:Apoptosis; D015415:Biomarkers; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D053938:DNA Fragmentation; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008875:Middle Aged; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000473:pathology*; D009135:Muscular Diseases / Q000378:metabolism / Q000473:pathology; D009223:Myotonic Dystrophy / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins; D017285:Polymyositis / Q000378:metabolism / Q000473:pathology; D013946:Thymopoietins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D008565:Membrane Proteins; D009124:Muscle Proteins; D009687:Nuclear Proteins; D013946:Thymopoietins; C092710:emerin