title: Allergy to date fruits: characterization of antigens and allergens of fruits of the date palm (Phoenix dactylifera L.).
abstract: BACKGROUND
  Date-palm (Phoenix dactylifera L.) fruits are eaten daily by most inhabitants of the Middle East and the neighboring countries. Recent reports have indicated that dates are allergenic. This study aimed to investigate the antigenic and allergenic potential of date fruits.
  
  
  METHODS
  Date-fruit extracts from eight cultivars were evaluated in skin prick tests (SPT) in an atopic population, used to produce antisera, analyzed by SDS-PAGE, and fractionated by gel-filtration chromatography. Sera from SPT-positive individuals were evaluated by ELISA and RAST, and in anti-igE immunoblot experiments.
  
  
  RESULTS
  About 13% of patients were SPT-positive for at least two extracts. SDS-PAGE of whole extracts revealed 15-18 protein bands of 6.5->100 kDa, and Sephacryl S-200 fractions gave distinct peptide bands. RAST and anti-IgE ELISA gave a range of positive results, which could be abrogated when sera were preabsorbed with fruit extracts. IgE immunoblots of different extracts with pooled positive sera revealed different anti-IgE-binding immunoprints. All the positive sera from fruit-allergic and pollen-allergic individuals bound strongly to two anti-IgE reactive bands of 6.5 to 12-14 kDa and 28-33 kDa, respectively, and about 50% of sera bound to a 54-58-kDa band.
  
  
  CONCLUSIONS
  These results strongly indicate that 1) date-palm fruit is a potent allergen 2) sera from fruit-allergic as well as pollen-allergic patients recognize common fruit-specific epitopes 3) there is heterogeneity in patient responses to the different extracts.
authors.affiliation: Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
authors.forename: A A
authors.initials: AA
authors.lastname: Kwaasi
authors.forename: H A
authors.initials: HA
authors.lastname: Harfi
authors.forename: R S
authors.initials: RS
authors.lastname: Parhar
authors.forename: S T
authors.initials: ST
authors.lastname: Al-Sedairy
authors.forename: K S
authors.initials: KS
authors.lastname: Collison
authors.forename: R C
authors.initials: RC
authors.lastname: Panzani
authors.forename: F A
authors.initials: FA
authors.lastname: Al-Mohanna
mesh_terms: D000485:Allergens / Q000009:adverse effects* / Q000737:chemistry / Q000276:immunology; D000941:Antigens / Q000009:adverse effects* / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005512:Food Hypersensitivity; D005638:Fruit / Q000009:adverse effects* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D010936:Plant Extracts / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology; D011852:Radioallergosorbent Test; D012882:Skin Tests
chemical_list: D000485:Allergens; D000941:Antigens; D010936:Plant Extracts; D007073:Immunoglobulin E