title: Adsorption of human IgG on Cu(2+)-immobilized cellulose affinity membrane: preliminary study.
abstract: Immobilized metal ion affinity chromatography (IMAC) is widely used. Transition metal ions have a high affinity to some peptide sequences. We have studied the selective adsorption of human IgG from a mixture of albumin, gamma-globulin, fibrinogen, and IgG onto Cu(2+) ion-immobilized cellulose membrane. Although Cu(2+) ligand is selective to IgG, in general gamma-globulins also are adsorbed. The simplicity and lower cost of Cu(2+) ion-immobilized cellulose membranes may be useful for removing IgG from blood.
authors.affiliation: Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram 695 012, India.
authors.forename: P R
authors.initials: PR
authors.lastname: Hari
authors.forename: W
authors.initials: W
authors.lastname: Paul
authors.forename: C P
authors.initials: CP
authors.lastname: Sharma
mesh_terms: D000915:Antibody Affinity; D001672:Biocompatible Materials; D002482:Cellulose; D003300:Copper; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008567:Membranes, Artificial
chemical_list: D001672:Biocompatible Materials; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D003300:Copper; D002482:Cellulose