title: Functional association between the nef gene product and gag-pol region of HIV-1.
abstract: Nef gene function is diverse among virus isolates of primate immunodeficiency viruses. We found differential effects of nef mutation on the virus replication between two HIV-1 clones, NL432 and LAI. The nef mutation in NL432 affected the infectivity more severely compared with that in LAI, although the Nef functions of both clones were comparable. Analysis with a series of chimeric viruses between NL432 and LAI revealed that the gag-pol region was responsible for the differential effect of nef mutation. The functional association between Nef and gag-pol suggested that one of the potential targets of Nef was located within the gag-pol region.
authors.affiliation: Department of Viral Oncology, Institute for Virus Research, Kyoto University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ono
authors.forename: Y
authors.initials: Y
authors.lastname: Iwatani
authors.forename: A
authors.initials: A
authors.lastname: Nishimura
authors.forename: A
authors.initials: A
authors.lastname: Ishimoto
authors.forename: H
authors.initials: H
authors.lastname: Sakai
mesh_terms: D002460:Cell Line; D015685:Fusion Proteins, gag-pol / Q000235:genetics / Q000378:metabolism*; D015693:Gene Products, nef / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D011485:Protein Binding; D014771:Virion; D054311:nef Gene Products, Human Immunodeficiency Virus
chemical_list: D015685:Fusion Proteins, gag-pol; D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus