Enhanced expression of intercellular adhesion molecule-1 (ICAM-1) in amnion with term and preterm labour.
To evaluate the association between intercellular adhesion molecule-1 (ICAM-1) in the amnion and preterm labour and delivery, we have assessed ICAM-1 mRNA abundance by Northern analysis and protein levels by enzyme-linked immunosorbent assay (ELISA), in samples of this tissue after term and preterm delivery. The median ICAM-1 mRNA expression following preterm delivery (PTD, n=30) was 24 times greater (P< 0.05) than following elective caesarean section prior to labour at term (CST, n=14). ICAM-1 expression following vaginal delivery after spontaneous labour at term (SLT, n=11) was seven times greater than in the CST group (P< 0.05). The concentration of ICAM-1 protein in the PTD samples (n=31) was four-fold greater than (P< 0.05) in CST (n=14). It was also three-fold greater than in the SLT (n=15) samples (P< 0.05). The results were substantially the same when a preterm spontaneous labour group (PTL) (n=26), exclusive of deliveries complicated by pre-eclampsia (n=1) or intrauterine growth restriction (n=3), was compared to the CST and SLT groups. The ICAM-1 mRNA expression did not differ significantly (P=0.93) between PTL with (n=12) or without (n=14) indicators of intrauterine infection. The results were similar when ICAM-1 protein concentrations were compared (P=0.43) between these two groups. These findings indicate that ICAM-1 is expressed by the human amnion and that this expression is elevated with preterm labour and delivery.
Marvin
K W
KW
Department of Pharmacology and Clinical Pharmacology, University of Auckland, Auckland, New Zealand. k.marvin@auckland.ac.nz
Keelan
J A
JA
Sato
T A
TA
Coleman
M A
MA
McCowan
L M
LM
Miller
H C
HC
Mitchell
M D
MD
D000650:Amnion / Q000378:metabolism*; D002585:Cesarean Section; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics* / Q000378:metabolism*; D007743:Labor, Obstetric / Q000235:genetics* / Q000378:metabolism*; D007752:Obstetric Labor, Premature / Q000235:genetics* / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*
D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1