[The combined effects of MCYST and water organic pollutants on the co-induction of SHE cell transformation and expression of Ras P21 protein].
The authors studied the combined carcinogenic activities of MCYST and organic pollutants in water of Dianshan Lake, using in vitro Syrian hamster embryo (SHE) cell transformation and immunohistochemical assays. The results showed that MCYST alone could not induce SHE cell transformation, but it increased the effects of low dose organic pollutants in cell transformation in a dose-dependent manner. In the cells isolated from type II transformed foci, ras P21 protein showed a positive immunohistochemical staining. It suggests MCYST may be a tumor promoter, which has a synergistic carcinogenic activity organic pollutants in water and the activation of ras oncogene may be one of the factors inducing malignant transformation.
Wang
H
H
Department of Occupational Health, Beijing Medical University, China.
Zhu
H
H
Nie
L
L
Xu
J
J
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D003043:Cocarcinogenesis; D006224:Cricetinae; D004357:Drug Synergism; D004622:Embryo, Mammalian; D008647:Mesocricetus; D052998:Microcystins; D015689:Oncogene Protein p21(ras) / Q000096:biosynthesis*; D010456:Peptides, Cyclic / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D001427:Bacterial Toxins; D002273:Carcinogens; D052998:Microcystins; D010456:Peptides, Cyclic; D014874:Water Pollutants, Chemical; C078588:microcystin; D015689:Oncogene Protein p21(ras)