title: Neutrophil antigen 5b is carried by a protein, migrating from 70 to 95 kDa, and may be involved in neonatal alloimmune neutropenia.
abstract: BACKGROUND
  Neutrophil antigen 5b has been described as involved in transfusion reactions and not in neonatal alloimmune neutropenia.
  
  
  METHODS
  Anti-5b was found in the serum of a mother of a persistently neutropenic newborn, who had several bacterial infections. The neutropenia responded to treatment with recombinant human granulocyte-colony-stimulating factor. Immunoprecipitation experiments performed with this and three other 5b antisera identified a protein, migrating from 70 to 95 kDa, as carrier of 5b. The observed pattern of migration may point to heavy glycosylation of this protein.
  
  
  RESULTS
  Six 5b-negative donors were identified among 54 screened white donors, for a 5b gene frequency of 0.66.
  
  
  CONCLUSIONS
  Alloimmunization to 5b in pregnancy is rare. In the patients with neonatal neutropenia analyzed in the last decade, this was the first case discovered.
authors.affiliation: Central Laboratory of the Netherlands Red Cross, Amsterdam. M_de_Haas@clb.nl
authors.forename: M
authors.initials: M
authors.lastname: de Haas
authors.forename: E
authors.initials: E
authors.lastname: Muniz-Diaz
authors.forename: L G
authors.initials: LG
authors.lastname: Alonso
authors.forename: K
authors.initials: K
authors.lastname: van der Kolk
authors.forename: M
authors.initials: M
authors.lastname: Kos
authors.forename: L
authors.initials: L
authors.lastname: Buddelmeijer
authors.forename: L
authors.initials: L
authors.lastname: Porcelijn
authors.forename: A E
authors.initials: AE
authors.lastname: von dem Borne
mesh_terms: D000941:Antigens / Q000097:blood; D001782:Blood Donors; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D006801:Humans; D007154:Immune System Diseases / Q000097:blood; D016130:Immunophenotyping; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000276:immunology*; D008297:Male; D009503:Neutropenia / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D011233:Precipitin Tests; D011247:Pregnancy; D013290:Streptococcal Infections / Q000097:blood
chemical_list: D000941:Antigens; D016179:Granulocyte Colony-Stimulating Factor