title: Use of the confocal microscope to determine polyomavirus recombinant capsid-like particle entry into mouse 3T6 cells.
abstract: The structural protein genes of polyomavirus were expressed in the baculovirus system, and the proteins were found to assemble into capsid-like particles capable of packaging insect cell DNA. Recombinant capsid-like particles could be produced that were composed of the various structural proteins (VP1, VP1/2, VP1/3 and VP1/2 + VP3). Laser scanning confocal microscopy was used to determine if the various capsid-like particles could infect (enter) mouse 3T6 cells. Each of the various capsid-like particles was equally capable of cell entry as determined by indirect immunofluorescence confocal microscopy.
authors.affiliation: Division of Biology-Ackert Hall, Section of Virology and Oncology, Kansas State University, Manhattan 66506, USA.
authors.forename: K
authors.initials: K
authors.lastname: An
authors.forename: T R
authors.initials: TR
authors.lastname: Lovgren
authors.forename: M B
authors.initials: MB
authors.lastname: Tilley
authors.forename: R A
authors.initials: RA
authors.lastname: Consigli
mesh_terms: D000818:Animals; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D011120:Polyomavirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D015678:Viral Structural Proteins