Iron-chelating activity of tetracyclines and its impact on the susceptibility of Actinobacillus actinomycetemcomitans to these antibiotics.
Three tetracyclines (tetracycline, doxycycline, and minocycline) were found to possess iron-chelating activity in a colorimetric siderophore assay. Determination of MICs indicated that the activity of doxycycline against the periodontopathogen Actinobacillus actinomycetemcomitans was only slightly influenced by the presence of an excess of iron that likely saturates the antibiotic. On the other hand, the MICs of doxycycline and minocycline were significantly lower for A. actinomycetemcomitans cultivated under iron-poor conditions than under iron-rich conditions.
Grenier
D
D
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Québec, Canada. Daniel.Grenier@greb.ulaval.ca
Huot
M P
MP
Mayrand
D
D
D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D003470:Culture Media; D006801:Humans; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010510:Periodontal Diseases / Q000382:microbiology; D013754:Tetracyclines
D000900:Anti-Bacterial Agents; D003470:Culture Media; D007502:Iron Chelating Agents; D013754:Tetracyclines; D007501:Iron