title: [Trypanosoma cruzi genome: transcriptional mapping and karyotype correlation; molecular characterization of a surface antigen from the Tc13 family].
abstract: We describe herein the genome mapping of Trypanosoma cruzi, the etiological agent of Chagas' disease, by hybridization of a cosmid library gridded in high density filters with cDNA from an epimastigote expression library as probes. We also show the correlation of some cosmid contigs with parasite chromosomal bands. With libraries from the T. cruzi Genome Project we have characterized a new member of the Tc13 family belonging to the superfamily of trypomastigote surface antigens. Starting with a Tulahuén strain (Tul 2 stock) clone, homologous to these antigens, we have sequenced and characterized the complete gene in the CL Strain (CL Brener clone). We have also found homologies with different ESTs which characterization would lead to further knowledge of this antigen family.
authors.affiliation: Instituto Nacional de Parasitología Dr. Mario Fatala Chabén, ANLIS Malbrán, Buenos Aires, Argentina.
authors.forename: J
authors.initials: J
authors.lastname: Bua
authors.forename: G A
authors.initials: GA
authors.lastname: García
authors.forename: M
authors.initials: M
authors.lastname: Galindo
authors.forename: N
authors.initials: N
authors.lastname: Galanti
authors.forename: A M
authors.initials: AM
authors.lastname: Ruiz
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D015342:DNA Probes; D015723:Gene Library; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015342:DNA Probes