Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae.
Parasites of the genus Plasmodium are transmitted to mammalian hosts by anopheline mosquitoes. Within the insect vector, parasite growth and development are potentially limited by antimicrobial defence molecules. Here, we describe the isolation of cDNA and genomic clones encoding a cecropin antibacterial peptide from the malaria vector mosquito Anopheles gambiae. The locus was mapped to polytene division 1C of the X chromosome. Cecropin RNA was induced by infection with bacteria and Plasmodium. RNA levels varied in different body parts of the adult mosquito. During development, cecropin expression was limited to the early pupal stage. The peptide was purified from both adult mosquitoes and cell culture supernatants. Anopheles gambiae synthetic cecropins displayed activity against Gram-negative and Gram-positive bacteria, filamentous fungi and yeasts.
Vizioli
J
J
UPR CNRS 9022, Institut de Biologie Moléculaire et Cellulaire, 15, rue René Descartes, Strasbourg Cedex, France.
Bulet
P
P
Charlet
M
M
Lowenberger
C
C
Blass
C
C
Müller
H M
HM
Dimopoulos
G
G
Hoffmann
J
J
Kafatos
F C
FC
Richman
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001483:Base Sequence; D003001:Cloning, Molecular; D019476:Insect Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry
D000900:Anti-Bacterial Agents; D019476:Insect Proteins; C406844:cecropin protein, Anopheles; D012313:RNA