Streptococcus gordonii strains resistant to fluorodeoxyuridine contain mutations in the thymidine kinase gene and are deficient in thymidine kinase activity.
Mutants of Streptococcus gordonii resistant to 5-fluorodeoxyuridine (FUdR(r)) were isolated. Each strain contained a point mutation resulting in the premature termination of the thymidine kinase (TK) open reading frame (tdk). In vitro translation of the mutant tdk coding regions resulted in synthesis of truncated TK polypeptides deficient in TK activity.
Franke
C A
CA
SIGA Pharmaceuticals, Inc., Corvallis, Oregon, USA.
Bolman
T M
TM
Ottum
S A
SA
Jones
K F
KF
Hruby
D E
DE
D000818:Animals; D004352:Drug Resistance, Microbial; D005467:Floxuridine / Q000494:pharmacology*; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D011817:Rabbits; D013291:Streptococcus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics* / Q000378:metabolism*
D005467:Floxuridine; D013937:Thymidine Kinase