Uveoscleral outflow in dog's eye: role of several enzymes.
The morphological pattern of several enzymes (succinic dehydrogenase--SDH, glucose-6-phosphate dehydrogenase--G6PDH and lactic dehydrogenase--LDH) was evaluated in normal dog eyes. Special attention was paid to the uveo-scleral tissue. Cryostatic sections of dog eye were stained with toluidine blue for the recognition of the microanatomical details or with histoenzymatic methods for SDH, G6PDH and LDH activities using sodium succinate, glucose-6-phosphate and sodium lactate as substrates respectively, nicotinamide adenine dinucleotide (NAD) as a reducing agent and sodium nitro-blue-tetrazolium as a colouring substance. A moderate positive reaction for SDH and a strong positive reaction for LDH were observed in the uveoscleral tissue, while G6PDH gave negative staining. Some considerations regarding a possible active role of these enzymatic activities to the aqueous humor outflow are suggested.
Cavallotti
C
C
Eye Clinic, University La Sapienza, Rome, Italy. cameroni@uniroma 1.it
Pescosolido
N
N
Artico
M
M
Cavallotti
D
D
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D004285:Dogs; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D012590:Sclera / Q000166:cytology / Q000201:enzymology*; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D014602:Uvea / Q000166:cytology / Q000201:enzymology*
D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase