IFN-beta modulates specific T cell responses in vitro but does not affect Experimental Autoimmune Encephalomyelitis in the SJL mouse.
In this study, mouse recombinant IFN-beta was shown to favor PLP139-151-specific Th2 responses in vitro, by inhibiting IFN-gamma production and stimulating IL-4 and IL-10 production. IFN-beta (5000 U/day) failed to prevent the development or severity of EAE induced with PLP139-151. Whereas efficacy of IL-10 was found in the B. pertussis assisted but not in the pertussigen-assisted EAE model, both models appeared insensitive to IFN-beta. Also the combination of (suboptimal) IL-10 and IFN-beta appeared ineffective in inhibiting disease. However, the PLP139-151-specific IL-10 production by T cells from these mice appeared significantly more sensitive to the stimulatory effect of IFN-beta in vitro. It is concluded that despite its Th2 promoting effects, IFN-beta is not effective in inhibiting EAE in this study.
Luca
M E
ME
Division of Immunological and Infectious Diseases, TNO Prevention and Health, Leiden, Netherlands. me.luca@pg.tno.nl
Visser
L
L
Lucas
C J
CJ
Nagelkerken
L
L
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016899:Interferon-beta / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D037342:Pertussis Toxin; D011994:Recombinant Proteins / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; D016753:Interleukin-10; D016899:Interferon-beta; D037342:Pertussis Toxin