Characterization of cytostatically active glycosphingolipids isolated from thioglycollate-elicited murine macrophages.
Two acidic glycosphingolipids (gangliosides) derived from mouse macrophage membranes and separated by thin-layer chromatography have a strong cytostatic effect on human and mouse tumor cells. The structure of the two gangliosides, named M phi G1 and M phi G2, was elucidated by application of physicochemical and immunochemical methods. Gas chromatography and mass spectrometry of M phi G1 and M phi G2 classified them as isomeric monosialogangliosides with ceramide moieties composed of sphingosine as the long-chain base, C16 and C18 fatty acids, respectively, and a lacto-tetraose backbone. For M phi G1, additional immunochemical findings led to the proposed structure IV3NeuAc-nLcOse4Cer. The immunochemical reactions of M phi G2 suggest a branched structure for the oligosaccharide moiety.
Schaade
L
L
Department of Medical Microbiology, RWTH Aachen, Germany. lschaade@alpha.imib.rwth-aachen.de
Ritter
K
K
Schiebel
H M
HM
Thomssen
R
R
Kleines
M
M
D000818:Animals; D006028:Glycosphingolipids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D013864:Thioglycolates
D006028:Glycosphingolipids; D013864:Thioglycolates