title: Induction of tcI 7, a gene encoding a beta-subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide.
abstract: We previously isolated, by differential display and 5' RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for tobacco cryptogein Induced), a gene encoding a beta-subunit of proteasome. Here, we report that tcl 7 was up-regulated in tobacco plants treated with elicitins (cryptogein and parasiticein) that have been shown to induce a systemic acquired resistance (SAR). Moreover, subsequent inoculation of tobacco with the pathogen Phytophthora parasitica var. nicotianae (Ppn) was shown to induce an additional activation of tcI 7 in tobacco plants pretreated with cryptogein. We also showed an up-regulation of tcI 7 by salicylic acid (SA). Moreover, accumulation of tcI 7 transcripts after treatment with cryptogein or with SA only occurred in NahG 9-tobacco plants that do not express the salicylate hydroxylase and thus are able to accumulate SA and develop a SAR. Suppressed accumulation of tcI 7 transcripts in NahG 8+ tobacco plants after cryptogein or SA treatment correlated with the loss of SAR. H2O2 was also shown to up-regulate tcI 7 in tobacco plants. Using gene walking by PCR we cloned and sequenced the 5' flanking region of tcI 7 containing hypothetical regulatory sequences, especially myb and NF-kappaB boxes, that could be responsible for the regulation of tcI 7 by salicylic acid and H2O2 respectively.
authors.affiliation: Unité Associée INRA-Université de Bourgogne, Phytopharmacie et Biochimie des Interactions Cellulaires, Dijon, France.
authors.forename: P
authors.initials: P
authors.lastname: Etienne
authors.forename: A S
authors.initials: AS
authors.lastname: Petitot
authors.forename: V
authors.initials: V
authors.lastname: Houot
authors.forename: J P
authors.initials: JP
authors.lastname: Blein
authors.forename: L
authors.initials: L
authors.lastname: Suty
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics*; D018076:DNA, Complementary; D005656:Fungal Proteins / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D006861:Hydrogen Peroxide; D003546:Cysteine Endopeptidases; C404941:TcI 7 protein, Nicotiana tabacum; D020156:Salicylic Acid