Low frequency vibrations and structural characterization of a murine IgG2a monoclonal antibody studied by raman and IR spectroscopies.
The Raman and ir spectra of a murine IgG2a monoclonal antibody molecule are reported. In accordance with previous studies on immunoglobulins, the secondary structure is predominantly of the beta-sheet type. The low frequency region of the Raman spectrum was also analyzed in detail. A structured band with two maxima near 43 and 94 cm(-1) was observed. This band has been attributed to vibrations of elastic body. The results are interpreted using a simple elastic model.
Picquart
M
M
Groupe de Recherche en Physique et Biophysique (GRPB), Université René Descartes, UFR Biomédicale, 45 Rue des Saints-Pères, 75270 Paris Cedex 06, France.
Haro-Poniatowski
E
E
Morhange
J F
JF
Jouanne
M
M
Kanehisa
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G