Analysis of connexin intracellular transport and assembly.
Central to understanding the establishment and regulation of gap junction-mediated intercellular communication is a detailed knowledge of how these complex structures are assembled. In this article, we describe methods to modulate and/or monitor the transport of connexins from the endoplasmic reticulum to the Golgi complex and from the trans Golgi network to the cell surface. We also present techniques that we have developed to assay assembly of newly synthesized connexin monomers into connexons and gap junctional plaques.
VanSlyke
J K
JK
Vollum Institute, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97201, USA.
Musil
L S
LS
D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002450:Cell Communication; D002478:Cells, Cultured; D017630:Connexins / Q000096:biosynthesis / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D017629:Gap Junctions / Q000378:metabolism / Q000502:physiology*; D006056:Golgi Apparatus / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008854:Microscopy, Electron; D013462:Sulfur Radioisotopes
D017630:Connexins; D013462:Sulfur Radioisotopes; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D008715:Methionine