title: Bacterial contamination and sperm recovery after semen preparation by density gradient centrifugation using silane-coated silica particles at different g forces.
abstract: The effects of density gradient centrifugation through silane-coated silica particles (PureSperm) using 100, 200, 300 and 500 g on bacterial contamination of sperm samples and recovery of motile spermatozoa from sperm samples were investigated with conventional culturing techniques and microscopic visual assessment. The recovery of motile spermatozoa was variable and was not improved using 500 g compared to the recommended 300 g. The bacterial contamination was highly decreased by gradient centrifugation through PureSperm and was almost abolished when strict aseptic techniques were used, with changes to sterile Pasteur pipettes and tubes prior to washing procedures.
authors.affiliation: Department of Obstetrics and Gynecology, Umeå University Hospital, S-90185 Umeå, Sweden.
authors.forename: C M
authors.initials: CM
authors.lastname: Nicholson
authors.forename: L
authors.initials: L
authors.lastname: Abramsson
authors.forename: S E
authors.initials: SE
authors.lastname: Holm
authors.forename: E
authors.initials: E
authors.lastname: Bjurulf
mesh_terms: D000328:Adult; D001419:Bacteria; D002499:Centrifugation, Density Gradient / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012099:Reproductive Techniques; D012661:Semen / Q000502:physiology; D012822:Silicon Dioxide; D013081:Sperm Motility; D013094:Spermatozoa / Q000382:microbiology* / Q000502:physiology*; D013242:Sterilization / Q000379:methods
chemical_list: D012822:Silicon Dioxide