title: A standardized plaque reduction assay for determination of drug susceptibilities of cytomegalovirus clinical isolates.
abstract: Twelve laboratories collaborated in formulating and testing a standardized plaque reduction assay for cytomegalovirus (CMV) cell-associated clinical isolates. Four characterized and plaque-purified CMV strains, as well as six coded clinical isolates obtained after antiviral therapy, were distributed and tested. Good agreement was obtained for four of the clinical isolates, but a broad distribution of results was obtained for two isolates. Analysis of these results indicates the problems associated with clinical isolates, including the large genetic variability and the highly cell-associated phenotype. This collaborative effort, by addressing these problems, represents a significant step toward the development of a standardized assay.
authors.affiliation: Yale University, New Haven, Connecticut, USA. marie.landry@yale.edu
authors.forename: M L
authors.initials: ML
authors.lastname: Landry
authors.forename: S
authors.initials: S
authors.lastname: Stanat
authors.forename: K
authors.initials: K
authors.lastname: Biron
authors.forename: D
authors.initials: D
authors.lastname: Brambilla
authors.forename: W
authors.initials: W
authors.lastname: Britt
authors.forename: J
authors.initials: J
authors.lastname: Jokela
authors.forename: S
authors.initials: S
authors.lastname: Chou
authors.forename: W L
authors.initials: WL
authors.lastname: Drew
authors.forename: A
authors.initials: A
authors.lastname: Erice
authors.forename: B
authors.initials: B
authors.lastname: Gilliam
authors.forename: N
authors.initials: N
authors.lastname: Lurain
authors.forename: J
authors.initials: J
authors.lastname: Manischewitz
authors.forename: R
authors.initials: R
authors.lastname: Miner
authors.forename: M
authors.initials: M
authors.lastname: Nokta
authors.forename: P
authors.initials: P
authors.lastname: Reichelderfer
authors.forename: S
authors.initials: S
authors.lastname: Spector
authors.forename: A
authors.initials: A
authors.lastname: Weinberg
authors.forename: B
authors.initials: B
authors.lastname: Yen-Lieberman
authors.forename: C
authors.initials: C
authors.lastname: Crumpacker
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects* / Q000502:physiology; D003586:Cytomegalovirus Infections / Q000821:virology*; D005347:Fibroblasts / Q000821:virology; D017245:Foscarnet / Q000494:pharmacology; D015774:Ganciclovir / Q000494:pharmacology; D006801:Humans; D007753:Laboratories / Q000592:standards; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D010948:Viral Plaque Assay / Q000379:methods* / Q000592:standards*
chemical_list: D000998:Antiviral Agents; D017245:Foscarnet; D015774:Ganciclovir