[Immunoblots of hydrophobic OmpL39 of Leptospiral interrogans with immunoprotective Mb E4B7G5].
The immunoprotective Mb E4B7G5 against outer membrane antigens from L. interrogans serovar Lai strain 017 were produced and used in immunoblots of the OMP of six strains of L. interrogans (017, 601, 603, 609, 620 and 245). The OMP from the six strains, which partitioned into the hydrophobic detergent phase, contained four-seven major proteins bands of 66 kd-16 kd. It was found that Mb E4B7G5 recognized only specifically the 39 kd antigenic band of strain 017, 601, 603 and 609, and did not recognize apparently any bands of strains 620 and 245. The findings suggest that Mb E4B7G5 be valuable for separating protective antigen of OMP and studying genetic vaccines.
Yan
R
R
Research Unit of Leptospira, School of Basic Medical Sciences, Chengdu.
Li
Y
Y
Zhang
Y
Y
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007921:Leptospira interrogans / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins