title: Experimental trials with a thermostable Newcastle disease virus (strain I2) in commercial and village chickens in Tanzania.
abstract: Antibody responses in indigenous village and commercial chickens vaccinated with 12 thermostable Newcastle disease (ND) vaccine and protection levels against challenge with a virulent field isolate were determined. The antibody response of village chickens vaccinated by eye drop revealed that 30, 60 and 90 days after primary vaccination, the mean log2 HI titres were 6.1, 5.4 and 3.6, respectively, whereas for commercial chickens, the antibody response after 14, 30 and 90 days were 8.2, 5.1 and 4.2, respectively. Village chickens vaccinated orally via drinking water had mean log2 HI titres of 3.4 after 30 days. After booster vaccination, the mean HI titre was 5.4 and 3.3 after 30 and 60 days post-secondary vaccination (i.e. 60 and 90 days after primary vaccination). Antibody response of mean log2 HI titres of 2.6 was recorded 30 days after primary vaccination orally through food; 30 and 60 days after secondary vaccination (i.e. 60 and 90 days after primary vaccination), mean log2 HI titres were 5.3 and 3.2, respectively. All commercial and village chickens vaccinated by eye drop survived the challenge trial whereas village chickens vaccinated through drinking water and food had protection levels of 80% and 60% 30 days after primary vaccination, respectively. However, 30 days after booster vaccination, the protection level was 100%. At 60 days after secondary vaccination, the protection level dropped again to 80% for chickens vaccinated orally. All control chickens used in the challenge trials developed clinical ND and died 3-5 days after inoculation with the virulent virus. Supported by laboratory findings, I2 strain of NDV seemed to be avirulent, immunogenic and highly protective against virulent isolates of NDV. It may be a suitable vaccine to use in village chickens to vaccinate them against ND in rural areas.
authors.affiliation: Animal Diseases Research Institute, Dar es Salaam, Tanzania. p.wambura@mailbox.uq.edu.au
authors.forename: P N
authors.initials: PN
authors.lastname: Wambura
authors.forename: A M
authors.initials: AM
authors.lastname: Kapaga
authors.forename: J M
authors.initials: JM
authors.lastname: Hyera
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002645:Chickens; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000472:pathogenicity*; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D013696:Temperature; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines
chemical_list: D014765:Viral Vaccines