Structure, expression, and chromosome mapping of LATS2, a mammalian homologue of the Drosophila tumor suppressor gene lats/warts.
We have cloned and characterized LATS2, a novel mammalian homologue of the Drosophila tumor suppressor gene lats/warts. Northern blot analysis showed ubiquitous expression of mouse LATS2 (MmLATS2) mRNA, whereas expression of human LATS2 (HsLATS2) mRNA was enhanced in skeletal muscle and heart. Immunoblotting analysis of fractionated cell lysates showed HsLats2 to be a nuclear protein. We mapped the MmLATS2 gene to mouse chromosome 14 by interspecific backcross analysis. We also mapped the HsLATS2 gene (by fluorescence in situ hybridization) to the 13q11-q12 region, in which a loss of heterozygosity has been frequently observed in many primary cancers and to which the tumor suppressor genes RB and BRCA2 have also been mapped.
Yabuta
N
N
Department of Molecular Genetics, Department of Science for Laboratory Animal Experimentation, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Fujii
T
T
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Nishiguchi
H
H
Endo
Y
Y
Toji
S
S
Tanaka
H
H
Nishimune
Y
Y
Nojima
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D015870:Gene Expression; D017344:Genes, Insect; D016147:Genes, Tumor Suppressor; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins
D018076:DNA, Complementary; D029721:Drosophila Proteins; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D011494:Protein Kinases; C092745:LATS1 protein, human; C490501:Lats1 protein, mouse; C093503:wts protein, Drosophila; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases