[Expression of p53 and ras p21 gene products in malignant transformed V79 cell induced by various organic components of DEPs].
The effect of various fractions of diesel exhausted particles on the expression of mutant p53 antigen product and ras oncogene p21 product was examined by immunohistochemical method in malignant transformed V79 cell. The result showed that all fractions of diesel exhausted particles (DEPs) could significantly increase the expression of p53 products (P < 0.01). It demonstrated that the organic fractions of DEPs could induce the mutation of p53 suppressor gene in malignant transformed cells. But the expression levels of ras oncogene p21 product were not changed under the same experimental conditions (P > 0.05).
Lü
Y
Y
National Evaluation Centre for the Toxicology of Fertility Regulating Drugs, Shanghai, China.
Ding
X
X
Ye
S
S
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006224:Cricetinae; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D010316:Particle Size; D017354:Point Mutation; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D001335:Vehicle Emissions / Q000009:adverse effects*
D016159:Tumor Suppressor Protein p53; D001335:Vehicle Emissions; D016283:Proto-Oncogene Proteins p21(ras)