Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand.
A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.
Pandey
B D
BD
Department of Virology, Institute for Tropical Medicine, Nagasaki University, Nagasaki City, Japan. b-pandey@alpha.med.nagasaki-u.ac.jp
Karabatsos
N
N
Cropp
B
B
Tagaki
M
M
Tsuda
Y
Y
Ichinose
A
A
Igarashi
A
A
D000818:Animals; D003465:Culex / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005416:Flavivirus / Q000145:classification* / Q000302:isolation & purification* / Q000472:pathogenicity / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009500:Neutralization Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D013785:Thailand; D014773:Virology; D014776:Virus Cultivation
D004279:DNA, Viral