Vaccination of Manchego lambs against Haemonchus contortus with a somatic fraction (p26/23) of adult parasites.
A low molecular weight fraction from adult Haemonchus contortus containing two peptides (p26/23) was used to vaccinate Manchego female lambs between 3.5 and 5 months of age. Immunizing injections were given three times on days 0, 14 and 28 of the experiment. On day 43, lambs were challenged with 400 third stage larvae/kg live weight. Vaccination induced a lengthening of prepatent periods, significant reduction (> 60%) in mean faecal egg counts and smaller variations in packed cell volume values. At necropsy, average worm burden in the vaccinated lambs was significantly lower (61.6%) than that found in unvaccinated challenged animals. A clear correlation was found between protection and serum antibody response in immunized lambs.
Domínguez-Toraño
I A
IA
Departamento de Patologia Animal I, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
Cuquerella
M
M
Gómez-Muñoz
M
M
Mendez
S
S
Fernández-Pérez
F J
FJ
Alunda
J M
JM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000737:chemistry / Q000276:immunology*; D015801:Helminth Proteins / Q000008:administration & dosage / Q000276:immunology; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D010455:Peptides; D014612:Vaccines