title: Eosinophil-derived transforming growth factors (TGF-alpha and TGF-beta 1) in human periradicular lesions.
abstract: Inflammatory mediators of periradicular lesions are poorly understood. Transforming growth factors-alpha and -beta 1 (TGF-alpha and TGF-beta 1) have been linked with the cellular processes for both soft and hard tissue wound healing. The purpose of this study is to demonstrate the cellular sources of TGF-alpha and TGF-beta 1 mRNA and protein in periapical lesions by in situ hybridization and immunohistochemistry. Nine periapical granulomas and nine periapical cysts were examined. TGF-alpha mRNA and protein were not detectable in the granulomas examined. However, eosinophils surrounding the periapical cysts demonstrated both TGF-alpha mRNA and protein. The vast majority of eosinophils present in the periapical granulomas and cysts also demonstrated TGF-beta 1 mRNA and protein. Other cells producing TGF-beta 1 were lymphocytes, fibroblasts, and monocytes. The presence of wound repair cytokines, such as TGF-alpha and TGF-beta 1, suggests a mechanism by which the host inflammatory response may participate in the repair and remodeling of periapical tissues.
authors.affiliation: Tufts School of Dental Medicine, USA.
authors.forename: L W
authors.initials: LW
authors.lastname: Tyler
authors.forename: K
authors.initials: K
authors.lastname: Matossian
authors.forename: R
authors.initials: R
authors.lastname: Todd
authors.forename: G T
authors.initials: GT
authors.lastname: Gallagher
authors.forename: R R
authors.initials: RR
authors.lastname: White
authors.forename: D T
authors.initials: DT
authors.lastname: Wong
mesh_terms: D000328:Adult; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D018836:Inflammation Mediators; D008297:Male; D010484:Periapical Granuloma / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D011842:Radicular Cyst / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D014945:Wound Healing / Q000276:immunology
chemical_list: D018836:Inflammation Mediators; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta