[Study on the relationship between deoxyribonucleoside triphosphate (dNTP) pools and cell transformation].
Deoxyribonucleoside triphosphate (dNTP) pools were measured in normal BALB/c3T3 cells, transformation-treated cells and transformed cells with reverse-phase HPLC. The fluctuation of dNTP pools was similar after cells were treated with alkylating mutagens glycidyl methacrylate (GMA) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The gap between (dGTP + dATP) pools and (dTTP + dCTP) pools was greatly intensified. The measurements also indicated that the dNTP pools in transformed cells were quite different from those in normal cells. The results suggest that dNTP pools may play an important role in cell transformation.
Lei
H
H
Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing, China.
Li
Z
Z
Xie
D
D
Liu
B
B
D016475:3T3 Cells / Q000378:metabolism / Q000473:pathology; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate; D002471:Cell Transformation, Neoplastic; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003838:Deoxyadenine Nucleotides / Q000378:metabolism*; D003845:Deoxycytosine Nucleotides / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine; D051379:Mice; D008807:Mice, Inbred BALB C; D009153:Mutagens
D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; C033845:5-iodocytosine deoxyribonucleoside triphosphate; D017438:Bisphenol A-Glycidyl Methacrylate; C033846:2-amino-2'-deoxyadenosine 5'-triphosphate