title: Different fungal manganese-oxidizing peroxidases: a comparison between Bjerkandera sp. and Phanerochaete chrysosporium.
abstract: Two manganese-oxidizing peroxidases differing in glycosylation degree were purified from fermenter cultures of Bjerkandera sp. They were characterized and compared with the three manganese-oxidizing peroxidase isoenzymes obtained from the well-known ligninolytic fungus Phanerochaete chrysosporium. All the enzymes showed similar molecular masses but those from P. chrysosporium had less acidic isoelectric point. Moreover, the latter strictly required Mn2+ to oxidize phenolic substrates whereas the Bjerkandera peroxidases had both Mn-mediated and Mn-independent activity on phenolic and non-phenolic aromatic substrates. Taking into account these results, and those reported for Bjerkandera adusta and different Pleurotus species, we concluded that two different types of Mn(2+)-oxidizing peroxidases are secreted by ligninolytic fungi.
authors.affiliation: Department of Chemical Engineering, University of Santiago de Compostela, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Palma
authors.forename: A T
authors.initials: AT
authors.lastname: Martínez
authors.forename: J M
authors.initials: JM
authors.lastname: Lema
authors.forename: M J
authors.initials: MJ
authors.lastname: Martínez
mesh_terms: D000595:Amino Acid Sequence; D002384:Catalysis; D006031:Glycosylation; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism*; D008345:Manganese / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology*; D011124:Polyporaceae / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D008345:Manganese; D010544:Peroxidases