Detection of a mammalian histone H4 kinase that has yeast histidine kinase-like enzymic activity.
A well characterized histidine kinase purified from yeast has been shown to phosphorylate histone H4 on a histidine residue. This enzyme is unlike the two-component histidine kinases predominantly found in prokaryotes. Until now, a histidine kinase similar to this yeast enzyme has not been purified from a mammalian source. By using a purification scheme similar to that used to purify the yeast histidine kinase, a protein fraction with histone H4 kinase activity has been isolated from porcine thymus. The yeast histidine kinase was shown to be detectable using an in-gel kinase assay system and using this system, four major bands of histone H4 kinase activity were apparent in the porcine thymus preparation. Through the use of immunoprecipitation, alkaline hydrolysis and subsequent phosphoamino acid analysis it has been demonstrated that this partially purified kinase fraction is capable of phosphorylating histone H4 on histidine. In conclusion, an preparation has been made from porcine thymus that contains histone H4 kinase activity and at least one of the kinases present in this preparation is a histidine kinase.
Besant
P G
PG
Department of Biochemistry, The University of Western Australia, Nedlands, Australia.
Attwood
P V
PV
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D000071677:Histidine Kinase; D006657:Histones / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D010766:Phosphorylation; D006656:Protamine Kinase / Q000302:isolation & purification* / Q000378:metabolism*; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013552:Swine; D013950:Thymus Gland / Q000201:enzymology
D000596:Amino Acids; D006657:Histones; D011494:Protein Kinases; D006656:Protamine Kinase; D000071677:Histidine Kinase