title: Direct evidence for increased hydroxyl radicals originating from superoxide in the failing myocardium.
abstract: Experimental and clinical studies have suggested an increased production of reactive oxygen species (ROS) in the failing myocardium. The present study aimed to obtain direct evidence for increased ROS and to determine the contribution of superoxide anion (*O(2)(-)), H(2)O(2), and hydroxy radical (*OH) in failing myocardial tissue. Heart failure was produced in adult mongrel dogs by rapid ventricular pacing at 240 bpm for 4 weeks. To assess the production of ROS directly, freeze-clamped myocardial tissue homogenates were reacted with the nitroxide radical, 4-hydroxy-2,2,6, 6,-tetramethyl-piperidine-N-oxyl, and its spin signals were detected by electron spin resonance spectroscopy. The rate of electron spin resonance signal decay, proportional to *OH level, was significantly increased in heart failure, which was inhibited by the addition of dimethylthiourea (*OH scavenger) into the reaction mixture. Increased *OH in the failing heart was abolished to the same extent in the presence of desferrioxamine (iron chelator), catalase (H(2)O(2) scavenger), and 4,5-dihydroxy-1,3-benzene disulfonic acid (Tiron; LaMotte) (*O(2)(-) scavenger), indicating that *OH originated from H(2)O(2) and *O(2)(-). Further, *O(2)(-) produced in normal myocardium in the presence of antimycin A (mitochondrial complex III inhibitor) could reproduce the increase of H(2)O(2) and *OH seen in the failing tissue. There was a significant positive relation between myocardial ROS level and left ventricular contractile dysfunction. In conclusion, in the failing myocardium, *OH was produced as a reactive product of *O(2)(-) and H(2)O(2), which might play an important role in left ventricular failure.
authors.affiliation: Cardiovascular Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ide
authors.forename: H
authors.initials: H
authors.lastname: Tsutsui
authors.forename: S
authors.initials: S
authors.lastname: Kinugawa
authors.forename: N
authors.initials: N
authors.lastname: Suematsu
authors.forename: S
authors.initials: S
authors.lastname: Hayashidani
authors.forename: K
authors.initials: K
authors.lastname: Ichikawa
authors.forename: H
authors.initials: H
authors.lastname: Utsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Machida
authors.forename: K
authors.initials: K
authors.lastname: Egashira
authors.forename: A
authors.initials: A
authors.lastname: Takeshita
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004195:Disease Models, Animal; D004285:Dogs; D004578:Electron Spin Resonance Spectroscopy / Q000592:standards; D006333:Heart Failure / Q000378:metabolism* / Q000503:physiopathology; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000378:metabolism; D010138:Pacemaker, Artificial; D015203:Reproducibility of Results; D013481:Superoxides / Q000378:metabolism*; D018487:Ventricular Dysfunction, Left / Q000378:metabolism / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide