Cloning and nucleotide sequence of a gene encoding a glycogen debranching enzyme in the trehalose operon from Arthrobacter sp. Q36.
A gene located just upstream of the treYZ operon was isolated from Arthrobacter sp. strain Q36. The gene, designated treX, encoded an 823-amino acid protein. The amino acid sequence of the protein had 50% identity with the TreX protein (isoamylase) from Sulfolobus acidocaldarius ATCC 33909 which has a treZXY operon on the genome. We suggest that Arthrobacter treX is an isoamylase gene, and that it is a component of a treXYZ operon.
Maruta
K
K
Hayashibara Biochemical Laboratories, Inc., 7-7 Amase-minami machi, Okayama, Japan. amaseken@hayahshibara.co.jp
Kubota
M
M
Fukuda
S
S
Kurimoto
M
M
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006003:Glycogen / Q000378:metabolism; D006004:Glycogen Debranching Enzyme System / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon
D006004:Glycogen Debranching Enzyme System; D006003:Glycogen