title: KSHV-encoded CC chemokine vMIP-III is a CCR4 agonist, stimulates angiogenesis, and selectively chemoattracts TH2 cells.
abstract: Kaposi's sarcoma-associated herpesvirus (KSHV) encodes 3 genes that are homologous to cellular chemokines. vMIP-III, the product of open reading frame K4.1, is the most distantly related to human chemokines and has yet to be characterized. We have examined the interaction of vMIP-III with chemokine receptors, its expression in KS lesions, and its in ovo angiogenic properties. We show expression of vMIP-III in KS lesions and demonstrate the stimulation of angiogenesis by this chemokine, like vMIP-I and vMIP-II, in the chick chorioallantoic membrane assay. vMIP-III does not block human immunodeficiency virus entry through the coreceptors CCR3, CCR5, or CXCR4. However, vMIP-III is an agonist for the cellular chemokine receptor CCR4. CCR4 is expressed by TH2-type T cells. Consistent with this, vMIP-III preferentially chemoattracts this cell type. Because of these biologic properties and because it is expressed in KS lesions, vMIP-III may play an important role in the pathobiology of KS. (Blood. 2000;95:1151-1157)
authors.affiliation: ICOS Corporation, Bothell, WA 98021, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Stine
authors.forename: C
authors.initials: C
authors.lastname: Wood
authors.forename: M
authors.initials: M
authors.lastname: Hill
authors.forename: A
authors.initials: A
authors.lastname: Epp
authors.forename: C J
authors.initials: CJ
authors.lastname: Raport
authors.forename: V L
authors.initials: VL
authors.lastname: Schweickart
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: G
authors.initials: G
authors.lastname: Simmons
authors.forename: C
authors.initials: C
authors.lastname: Boshoff
authors.forename: P
authors.initials: P
authors.lastname: Clapham
authors.forename: Y
authors.initials: Y
authors.lastname: Chang
authors.forename: P
authors.initials: P
authors.lastname: Moore
authors.forename: P W
authors.initials: PW
authors.lastname: Gray
authors.forename: D
authors.initials: D
authors.lastname: Chantry
mesh_terms: D000482:Allantois / Q000098:blood supply; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D019742:Chemokines, CC / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology* / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply; D006224:Cricetinae; D006678:HIV / Q000187:drug effects / Q000502:physiology; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D016366:Open Reading Frames; D054398:Receptors, CCR4; D019707:Receptors, Chemokine / Q000819:agonists*; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D012514:Sarcoma, Kaposi / Q000276:immunology / Q000473:pathology / Q000821:virology; D018417:Th1 Cells / Q000187:drug effects / Q000502:physiology; D018418:Th2 Cells / Q000187:drug effects / Q000502:physiology*; D014162:Transfection; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects
chemical_list: C515354:CCR4 protein, human; D019742:Chemokines, CC; D054398:Receptors, CCR4; D019707:Receptors, Chemokine; D011994:Recombinant Proteins; D014764:Viral Proteins; C404961:vMIP-III protein, Herpesvirus 8, Human