title: Lack of effect of ageing on acetate oxidation in rat skeletal muscle during starvation: a (13)C NMR study.
abstract: Acetate oxidation was examined by (13)C nuclear magnetic resonance in skeletal muscle from adult and old rats. Rats fasted for 5 days were perfused with [2-(13)C]acetate over 2 h, and muscle extracts were analyzed for [(13)C]glutamate isotopomers. This study shows that approximately 80 % of acetyl-coenzyme A entering the tricarboxylic cycle was derived from substrate infusion in both adult and old rats, and that the flux through anaplerotic pathways was approximately 21 % of the flux through citrate synthase. These data demonstrate that skeletal muscle from adult and old rats oxidizes the same proportion of exogenous acetate.
authors.affiliation: Centre de Recherche en Nutrition Humaine de Clermont-Ferrand and Institut National de la Recherche Agronomique, Unité d'Etude du Métabolisme Azoté, France.
authors.forename: V
authors.initials: V
authors.lastname: Mezzarobba
authors.forename: G
authors.initials: G
authors.lastname: Bielicki
authors.forename: F M
authors.initials: FM
authors.lastname: Jeffrey
authors.forename: M
authors.initials: M
authors.lastname: Mignon
authors.forename: J P
authors.initials: JP
authors.lastname: Renou
authors.forename: J
authors.initials: J
authors.lastname: Grizard
authors.forename: D
authors.initials: D
authors.lastname: Meynial-Denis
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000375:Aging; D000818:Animals; D005260:Female; D018698:Glutamic Acid / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D018482:Muscle, Skeletal / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013217:Starvation / Q000378:metabolism*
chemical_list: D000085:Acetates; D018698:Glutamic Acid