title: The expression of membrane-associated 67-kDa laminin receptor (67LR) is modulated in vitro by cell-contact inhibition.
abstract: The interaction of cells with their substratum is an important determinant of cell behaviour, influencing attachment, proliferation, and motility. Such interactions are mediated by cell surface receptors which bind to attachment factors, like the glycoprotein laminin in basement membranes. We have previously shown that expression of the 67-kDa laminin receptor (67LR) is elevated in proliferating retinal microvasculature compared with mature, quiescent vessels. Here, we examined 67LR mRNA and protein expression in primary cultures of retinal microvascular endothelial cells (RMEC) and in the breast cancer cell-line T47D during stages of contact inhibition. In both cell types, the expression levels of 67LR mRNA and membrane-associated 67LR protein were significantly increased during the proliferative phases of monolayer formation. As the cells achieved contact inhibition, 67LR expression was reduced to comparatively low levels. Thus, the differential expression of 67LR between dividing and contact-inhibited cells may indicate a role for this receptor during proliferative processes.
authors.affiliation: School of Biology and Biochemistry, Queen's University of Belfast, Belfast, BT9 7BL, Northern Ireland.
authors.forename: E A
authors.initials: EA
authors.lastname: Donaldson
authors.forename: D J
authors.initials: DJ
authors.lastname: McKenna
authors.forename: C B
authors.initials: CB
authors.lastname: McMullen
authors.forename: W N
authors.initials: WN
authors.lastname: Scott
authors.forename: A W
authors.initials: AW
authors.lastname: Stitt
authors.forename: J
authors.initials: J
authors.lastname: Nelson
mesh_terms: D015139:Blotting, Southern; D015153:Blotting, Western; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003260:Contact Inhibition / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004730:Endothelium, Vascular / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008833:Microcirculation / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D012333:RNA, Messenger / Q000378:metabolism; D017474:Receptors, Laminin / Q000096:biosynthesis* / Q000235:genetics*; D012160:Retina / Q000166:cytology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D007797:Laminin; D012333:RNA, Messenger; D017474:Receptors, Laminin