title: [Relationship between the expression level of alpha-MHC gene and cardiac contractility during heart failure].
abstract: To investigate the molecular basis of the decrease in myocardial contractility during heart failure, an animan model of heart failure was set up by means of deoxycorticosterone-acetate impregnated silicone rubber implants in wistar rats. Cardiac contractility in normal and heart failure rats was examined, and gene expression of its myocardial contractile protein, alpha-MHC, was quantitatively analyzed at gene transcription level by using RNA slot blot hybridization. The results showed that the cardiac contractility and the alpha-MHC mRNA levels in heart failure rats were all lower than those in the normal. Statistical analysis showed a positive correlation between the cardiac contractility and the gene expression of alpha-MHC (r = 0.4143, n = 43, P < 0.05). The above results indicate that gene expression level of alpha-MHC is one of the key factors determining cardiac contractility.
authors.forename: L
authors.initials: L
authors.lastname: Wei
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Ouyang
authors.forename: H
authors.initials: H
authors.lastname: Ying
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Qu
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002303:Cardiac Output, Low / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D015870:Gene Expression; D008297:Male; D009200:Myocardial Contraction; D018995:Myosin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D018995:Myosin Heavy Chains