Secretion of IL-12 by murine macrophages activated by immunoglobulin receptor-mediated internalization of the surface coat of Trichinella spiralis larvae.
Trichinella spiralis larvae incubated with a rabbit antiserum raised against the larval surface coat bound murine macrophages to the parasite surface. Cell binding was not observed without the antisurface coat serum, or with incubation of larvae in normal rabbit serum, or with antibodies to keyhole limpet haemocyanin which identify a cryptic T. spiralis larval antigen. Cell adherence to the larval surface was lost by treatment of the cells with the lysosomotropic drug primaquine, implicating a receptor-mediated mechanism. Cells adhering to the parasite surface internalized parasite surface coat material, which was subsequently concentrated into endosomes. Culture supernatants from these cells contained enhanced levels of IL-12. Thus, the initial Th1 response to T. spiralis infection may be explained by these data.
Modha
J
J
Division of Biochemistry and Molecular Biology, The Davidson Building, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK.
Piedrafita
D
D
Roberts
M C
MC
Kusel
J R
JR
Kennedy
M W
MW
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D002448:Cell Adhesion; D002460:Cell Line; D015801:Helminth Proteins / Q000276:immunology; D006790:Host-Parasite Interactions; D018664:Interleukin-12 / Q000096:biosynthesis*; D007814:Larva / Q000276:immunology; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011319:Primaquine / Q000494:pharmacology; D011817:Rabbits; D011971:Receptors, Immunologic / Q000276:immunology; D017160:Trichinella spiralis / Q000254:growth & development / Q000276:immunology*; D014235:Trichinellosis / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D018664:Interleukin-12; D011319:Primaquine