Evaluation of subtilisin cleavage specificity for RNase A peptide bonds by high-performance liquid chromatography and mass spectrometric analysis.
Méndez
T J
TJ
Department of Chemistry, University of Florida, Gainesville, Florida, 32611, USA.
Johnson
J V
JV
Richardson
D E
DE
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D066298:In Vitro Techniques; D013058:Mass Spectrometry / Q000379:methods*; D010179:Pancreas / Q000201:enzymology; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013381:Subtilisins / Q000378:metabolism*
D012259:Ribonuclease, Pancreatic; D013381:Subtilisins