No induction of apoptosis by IFN-beta in human antigen-specific T cells.
Interferon (IFN)-beta, the most effective immunomodulatory treatment for MS, inhibits the proliferation of myelin-specific T cells. We report that IFN-beta moderately enhances the expression of the death receptor, CD95, at the surface of human antigen-specific T cells. However, T-cell apoptosis was not induced by IFNbeta-1a or IFNbeta-1b as assessed by caspase activity or DNA fragmentation. Immunomodulation mediated by IFN-beta does not directly involve apoptotic pathways in human T cells.
Zipp
F
F
Department of Neurology, University Hospital Charité, Berlin, Germany.
Beyer
M
M
Gelderblom
H
H
Wernet
D
D
Zschenderlein
R
R
Weller
M
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology*; D053148:Caspase 3; D020169:Caspases / Q000276:immunology / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism / Q000494:pharmacology; D053938:DNA Fragmentation; D000939:Epitopes; D005456:Fluorescent Dyes / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D016899:Interferon-beta / Q000494:pharmacology*; D009103:Multiple Sclerosis / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D019014:fas Receptor / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; D003374:Coumarins; D000939:Epitopes; D005456:Fluorescent Dyes; D004676:Myelin Basic Protein; D009842:Oligopeptides; D012333:RNA, Messenger; C102580:acetyl-aspartyl-glutamyl-valyl-aspartyl-amino-4-methylcoumarin; D019014:fas Receptor; D016899:Interferon-beta; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases