title: The SH2-SH2-SH3 domain of phospholipase C-gamma1 directly binds to translational elongation factor-1alpha.
abstract: Phospholipase C-gamma1 (PLC-gamma1) is a lipase that hydrolyzes PIP2 to generate two second messengers, IP3 and DAG. By using the yeast two-hybrid system, we identified the translational elongation factor-1alpha (EF-1alpha) as a binding protein of PLC-gamma1 from the human B-lymphocyte library. Direct interaction between EF-1alpha and PLC-gamma1 was confirmed by the in vitro binding experiment using purified PLC-gamma1. Furthermore, from the in vitro binding experiment, we could demonstrate that the carboxyl terminal region of EF-1alpha is involved in the interaction with PLC-gamma1, and that both SH2 and SH3 domains of PLC-gamma1 are required for the interaction with EF-1alpha. In vivo interaction between EF-1alpha and PLC-gamma1 was confirmed by the immunoprecipitation experiment using anti-EF-1alpha antibody. The interaction between EF-1alpha and PLC-gamma1 was enhanced by EGF-treatment. Taken together, we suggest that EF-1alpha might play a role in PLC-gamma1-mediated signal transduction.
authors.affiliation: Department of Life Science and School of Environmental Engineering, Pohang University of Science and Technology, Korea.
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: F
authors.initials: F
authors.lastname: Si
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
authors.forename: S B
authors.initials: SB
authors.lastname: Hong
authors.forename: J I
authors.initials: JI
authors.lastname: Hwang
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: J S
authors.initials: JS
authors.lastname: Chang
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Ryu
authors.forename: P G
authors.initials: PG
authors.lastname: Suh
mesh_terms: D000818:Animals; D019556:COS Cells; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D051966:Phospholipase C gamma; D011233:Precipitin Tests; D011485:Protein Binding; D015398:Signal Transduction; D014162:Transfection; D010738:Type C Phospholipases / Q000378:metabolism*; D015003:Yeasts; D018909:src Homology Domains
chemical_list: D007527:Isoenzymes; D020648:Peptide Elongation Factor 1; D010738:Type C Phospholipases; D051966:Phospholipase C gamma