Reduced renal mass hypertension, but not high salt diet, alters skeletal muscle arteriolar distensibility and myogenic responses.
The effects of high salt diet and reduced renal mass hypertension (RRM-HT) on skeletal muscle arteriolar distensibility and myogenic responses were investigated in male Sprague-Dawley rats. Rats were enclosed in an air-tight box with the in situ cremaster muscle exteriorized and viewed via television microscopy. Normotensive rats were fed low salt (0.4% NaCl) or high salt (4.0% NaCl) diet and RRM-HT rats were fed high salt diet for 4-6 weeks. With the cremaster muscle superfused with either physiological salt solution (for myogenic responses) or Ca(2+)-free physiological salt solution (for arteriolar distensibility), box pressure (and therefore, intravascular pressure) was increased in 5 mm Hg increments to a maximum of +30 mm Hg. The myogenic responses of arterioles were not altered by high salt diet, but were enhanced with RRM-HT. Arteriolar distensibility was not affected by high salt diet, but was reduced in RRM-HT rats compared to either normotensive rat group. These data suggest that high salt diet does not significantly alter either myogenic responses or the distensibility of rat cremasteric arterioles. However, RRM-HT enhances myogenic responses of these vessels while reducing arteriolar distensibility. The impact of these effects must be taken into account when interpreting data describing alterations in skeletal muscle microvessel reactivity for animals on high salt diet or with RRM hypertension.
Frisbee
J C
JC
Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA. jfrisbee@mcw.edu
Lombard
J H
JH
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001160:Arterioles / Q000473:pathology; D001274:Atmospheric Pressure; D006973:Hypertension / Q000139:chemically induced / Q000209:etiology / Q000473:pathology*; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000473:pathology; D009131:Muscle, Smooth, Vascular / Q000473:pathology*; D009392:Nephrectomy / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017673:Sodium Chloride, Dietary / Q000633:toxicity*
D017673:Sodium Chloride, Dietary; D000241:Adenosine