A labelled avidin-biotin ELISA to detect antibodies to caprine arthritis-encephalitis virus in goats' sera.
A labelled avidin-biotin ELISA (lab-ELISA) was developed and compared with indirect ELISA (i-ELISA) and agar-gel immunodiffusion assay (AGID) for its efficacy in detecting antibodies against caprine arthritis-encephalitis virus (CAEV) in goat sera. The enzyme immunoassays were standardized using 113 sera from CAEV-negative goat flocks. The tests were compared using the results from 339 serum samples. The lab-ELISA showed the greatest number of positive results (94/339) as compared with AGID (51) and i-ELISA (64). The comparison of the other two tests with the lab-ELISA showed an agreement of 87.3% with AGID and 90.6% with i-ELISA. The lab-ELISA may be useful for screening large populations for CAEV antibodies, in epidemiological surveys and in the control of caprine arthritis-encephalitis.
Castro
R S
RS
Universidade Federal Rural de Pernambuco, Depto. de Medicina Veterinária, Recife, PE, Brazil.
Leite
R C
RC
Resende
M
M
Gouveia
A M
AM
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D016088:Arthritis-Encephalitis Virus, Caprine / Q000276:immunology*; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D015511:Goat Diseases / Q000276:immunology* / Q000821:virology; D006041:Goats; D016180:Lentivirus Infections / Q000276:immunology / Q000662:veterinary*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral