title: Interactions between Fc(epsilon)RI and lipid raft components are regulated by the actin cytoskeleton.
abstract: Previous studies showed that crosslinking of IgE-Fc(epsilon)RI complexes on RBL-2H3 mast cells causes their association with isolated detergent-resistant membranes, also known as lipid rafts, in a cholesterol-dependent process that precedes initiation of signaling by these receptors. To investigate these interactions on intact cells, we examined the co-redistribution of raft components with crosslinked IgE-Fc(epsilon)RI using confocal microscopy. After several hours of crosslinking at 4 degrees C, the glycosylphosphatidylinositol-linked protein Thy-1 and the Src-family tyrosine kinase Lyn co-redistribute with IgE-Fc(epsilon)RI in large patches at the plasma membrane. Under these conditions, F-actin also undergoes dramatic co-segregation with Fc(epsilon)RI and raft components but is dispersed following a brief warm-up to 37 degrees C. When crosslinking of IgE-Fc(epsilon)RI is initiated at higher temperatures, co-redistribution of raft components with patched Fc(epsilon)RI is not readily detected unless stimulated F-actin polymerization is inhibited by cytochalasin D. In parallel, cytochalasin D converts transient antigen-stimulated tyrosine phosphorylation to a more sustained response. Sucrose gradient analysis of lysed cells reveals that crosslinked IgE-Fc(epsilon)RI remains associated with lipid rafts throughout the time course of the transient phosphorylation response but undergoes a time-dependent shift to higher density that is prevented by cytochalasin D. Our results indicate that interactions between Lyn and crosslinked IgE-Fc(epsilon)RI are regulated by stimulated F-actin polymerization, and this is best explained by a segregation of anchored raft components from more mobile ones.
authors.affiliation: Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853-1301, USA. dah24@cornell.edu
authors.forename: D
authors.initials: D
authors.lastname: Holowka
authors.forename: E D
authors.initials: ED
authors.lastname: Sheets
authors.forename: B
authors.initials: B
authors.lastname: Baird
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D007073:Immunoglobulin E / Q000378:metabolism*; D050356:Lipid Metabolism; D008407:Mast Cells / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D017455:Receptors, IgE / Q000378:metabolism*
chemical_list: D000199:Actins; D017455:Receptors, IgE; D007073:Immunoglobulin E