title: Transcripts and sequence elements suggest differential promoter usage within the ycf3-psaAB gene cluster on mustard (Sinapis alba L.) chloroplast DNA.
abstract: The mustard chloroplast DNA region spanning the ycf3 gene and part of the psaAB operon was investigated. The ycf3 gene reveals two class-II introns that are removed during processing to give a mature 0.7-kb transcript, but no RNA editing seems to be involved. RNase protection and RT-PCR experiments suggest cotranscription of ycf3 with the downstream psaA gene, possibly from a NEP promoter upstream of ycf3, whereas distinct ycf3 and psaA transcripts are each initiated from PEP promoters. This situation is reminiscent of that for the trnK-psbA gene region. The implications for light-regulated versus light-independent expression of photosystem core-protein genes are discussed.
authors.affiliation: Arbeitsgruppe Pflanzliche Zellphysiologie und Molekularbiologie, Ruhr-Universität Bochum, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Summer
authors.forename: T
authors.initials: T
authors.lastname: Pfannschmidt
authors.forename: G
authors.initials: G
authors.lastname: Link
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018742:DNA, Chloroplast / Q000235:genetics*; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D007438:Introns / Q000235:genetics; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D009876:Operon / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000235:genetics; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000235:genetics; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology; D012326:RNA Splicing / Q000235:genetics; D018747:RNA, Chloroplast / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D016415:Sequence Alignment; D013728:Terminator Regions, Genetic / Q000235:genetics / Q000502:physiology; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D018742:DNA, Chloroplast; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D018747:RNA, Chloroplast; D012333:RNA, Messenger; C491600:Ycf3 protein, Sinapis alba; C089909:photosystem I, psaB subunit