Lack of effect of ageing on acetate oxidation in rat skeletal muscle during starvation: a (13)C NMR study.
Acetate oxidation was examined by (13)C nuclear magnetic resonance in skeletal muscle from adult and old rats. Rats fasted for 5 days were perfused with [2-(13)C]acetate over 2 h, and muscle extracts were analyzed for [(13)C]glutamate isotopomers. This study shows that approximately 80 % of acetyl-coenzyme A entering the tricarboxylic cycle was derived from substrate infusion in both adult and old rats, and that the flux through anaplerotic pathways was approximately 21 % of the flux through citrate synthase. These data demonstrate that skeletal muscle from adult and old rats oxidizes the same proportion of exogenous acetate.
Mezzarobba
V
V
Centre de Recherche en Nutrition Humaine de Clermont-Ferrand and Institut National de la Recherche Agronomique, Unité d'Etude du Métabolisme Azoté, France.
Bielicki
G
G
Jeffrey
F M
FM
Mignon
M
M
Renou
J P
JP
Grizard
J
J
Meynial-Denis
D
D
D000085:Acetates / Q000378:metabolism*; D000375:Aging; D000818:Animals; D005260:Female; D018698:Glutamic Acid / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D018482:Muscle, Skeletal / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013217:Starvation / Q000378:metabolism*
D000085:Acetates; D018698:Glutamic Acid