Expression of HLA-DR and its enhancing molecules in muscle fibers in polymyositis.
Polymyositis (PM) is an autoimmune inflammatory muscle disease of unknown cause in which cellular immunity is thought to play an important pathogenic role. Class II major histocompatibility complex (class II MHC: human leukocyte antigen (HLA)-DR operates as a cofactor of antigen presentation in immunological responses. There has been a major debate over whether muscle fibers themselves synthesize and express HLA-DR molecules and play a role in antigen presentation in PM pathogenesis. In this study, we demonstrated that most muscle fibers from patients with PM synthesized and expressed HLA-DR molecules on their surface. Human leukocyte antigen-DR expression was highly specific to PM. In addition, class II transactivator (CIITA), human leukocyte antigen DM (HLA-DM), and invariant chain (Ii), which are indispensable for expression of mature HLA-DR molecules and for antigen processing and presentation, were co-expressed. One of the cytokines that could induce this expression is interferon-gamma (IFN-gamma), released by activated lymphocytes. Our results indicate that in PM muscle fibers synthesize and express HLA-DR molecules and may contribute to the inflammatory responses together with lymphocytes.
Inukai
A
A
Department of Neurology, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
Kuru
S
S
Liang
Y
Y
Takano
A
A
Kobayashi
Y
Y
Sakai
M
M
Doyu
M
M
Sobue
G
G
D000328:Adult; D000368:Aged; D000906:Antibodies; D001706:Biopsy; D017931:DNA Primers; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D018485:Muscle Fibers, Skeletal / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D017285:Polymyositis / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000906:Antibodies; D017931:DNA Primers; D018076:DNA, Complementary; D006684:HLA-DR Antigens; D012333:RNA, Messenger