title: Clonal turnover of enterohemorrhagic Escherichia coli O157:H7 in experimentally infected cattle.
abstract: A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculation. The loss of the inoculated clone was observed in one calf. Replication and dissemination of the EHEC O157:H7 strains that mutated in cattle may result in the diversification of this organism among cattle populations.
authors.affiliation: Laboratory of Zoonosis, Feed Safety Research Division, National Institute of Animal Health, Kannondai 3-1-1, Tsukuba, Ibaraki, Japan. akiba@sat.affrc.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Akiba
authors.forename: T
authors.initials: T
authors.lastname: Sameshima
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005838:Genotype; D008297:Male; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial