title: Application of short tandem repeat of genomic DNA and mitochondrial DNA for identification of mixed-up tissue specimens.
abstract: Deoxyribonucleic acid (DNA) typing methods, short tandem repeat of genomic DNA and mitochondrial DNA with the use of polymerase chain reaction amplification, were applied to formalin-fixed, paraffin-embedded tissues submitted for diagnosis, to identify and sort out mixed-up tissue specimens. These techniques were found to be reliable, reproducible and specific for personal identification, and thus to eliminate the need for further examinations and to prevent unnecessary surgery.
authors.affiliation: Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan. kenjisa@sch.md.shinshu-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: K
authors.initials: K
authors.lastname: Takayanagi
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Kinoshita
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Asamura
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Fukushima
authors.forename: M
authors.initials: M
authors.lastname: Hotchi
mesh_terms: D000368:Aged; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D016678:Genome; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm