Effect of 2-mercaptoacetate and 2-deoxy-D-glucose administration on the expression of NPY, AGRP, POMC, MCH and hypocretin/orexin in the rat hypothalamus.
Using in situ hybridization, the mRNA levels encoding neuropeptide Y (NPY), agouti gene-related protein (AGRP), proopiomelanocortin (POMC), melanin-concentrating hormone (MCH) and hypocretin/orexin (HC/ORX) were investigated in the rat arcuate nucleus (Arc) and lateral hypothalamic area (LHA) 2 h after a single dose of the glucose antimetabolite 2-deoxy-D-glucose (2-DG; 600 mg/kg) or of the fatty acid oxidation inhibitor mercaptoacetate (MA; 600 mumol/kg). Two hours after 2-DG or MA injection food intake was significantly increased. NPY and AGRP mRNA levels in the Arc were increased by 2-DG but not affected by MA, and MCH mRNA levels in the LHA were increased by both antimetabolites. These results suggest that Arc neurons expressing NPY and AGRP are regulated by changes in glucose, but not fatty acid availability, whereas both factors affect MCH neurons in the LHA.
Sergeyev
V
V
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Broberger
C
C
Gorbatyuk
O
O
Hökfelt
T
T
D054369:Agouti-Related Protein; D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000378:metabolism; D002352:Carrier Proteins; D003847:Deoxyglucose / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D007028:Hypothalamic Hormones / Q000096:biosynthesis; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008543:Melanins / Q000096:biosynthesis; D009478:Neuropeptide Y / Q000096:biosynthesis; D009479:Neuropeptides / Q000096:biosynthesis*; D018377:Neurotransmitter Agents / Q000096:biosynthesis; D000068797:Orexins; D010907:Pituitary Hormones / Q000096:biosynthesis; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis; D014176:Protein Biosynthesis; D011506:Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013864:Thioglycolates / Q000494:pharmacology*
D054369:Agouti-Related Protein; D002352:Carrier Proteins; D007028:Hypothalamic Hormones; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009478:Neuropeptide Y; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D000068797:Orexins; D010907:Pituitary Hormones; D011506:Proteins; D012333:RNA, Messenger; D013864:Thioglycolates; D011333:Pro-Opiomelanocortin; C019463:melanin-concentrating hormone; C017487:2-mercaptoacetate; D003847:Deoxyglucose