title: Multiple site mutagenesis with high targeting efficiency in one cloning step.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139 USA. ygkim@mit.edu
authors.forename: Y G
authors.initials: YG
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Maas
mesh_terms: D001483:Base Sequence; D001709:Biotechnology; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D007763:Lac Operon; D016297:Mutagenesis, Site-Directed
chemical_list: D017931:DNA Primers; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific