Cloning, expression and characterization of a novel human Ras-related protein that is regulated by glucocorticoid hormone.
Ras proteins are a family of guanine nucleotide (GDP and GTP)-binding proteins that play central roles in essential signal transduction pathways. We have isolated in a yeast two-hybrid screen a human cDNA encoding a new protein that is highly homologous (98% identical at the protein level) to mouse DexRas1, a member of the Ras superfamily. The human DexRas1 is expressed in a variety of tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with the strongest expression in the heart. Using human fibrosarcoma HT-1080 cells as a model system, we show that the expression of human DexRas1 is stimulated by dexamethasone, suggesting a role of human DexRas1 in dexamethasone-induced alterations in cell morphology, growth and cell-extracellular matrix interactions.
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Department of Cell Biology and the Cell Adhesion and Matrix Research Center, University of Alabama at Birmingham, 35294-0019, USA.
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003907:Dexamethasone / Q000494:pharmacology*; D019204:GTP-Binding Proteins; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D018631:ras Proteins
C400132:RASD1 protein, human; D003907:Dexamethasone; D019204:GTP-Binding Proteins; C498511:Rasd1 protein, mouse; D020559:Monomeric GTP-Binding Proteins; D018631:ras Proteins