[Spatial and temporally expressed proteins during Drosophila oogenesis as revealed by monoclonal antibodies].
By use of hybridoma technique, we have prepared 6 monoclonal antibodies. As shown by antibody labeling of whole mount ovary, four of them recognized, respectively, the antigens which were spatial-temporally expressed during oogenesis. The B 2 antigens appeared very early in the germarium and were expressed mainly by cystocyte and nurse cells. Later, they were all transported and localized in the posterior of oocyte, F 9 antigens followed and were also transported and localized in the posterior of oocyte in the stage 7-8. Then, E 8 antigens appeared and mainly localized on the membrane of oocyte in the stage 9-10. C 3 antigens were expressed much later, at about stage 14. They localized like two caps in the perivitelline fluid at both ends of matured egg. Such specific expression and distribution pattern of all these antigens suggest their possible roles during oogenesis.
Song
Q B
QB
Shanghai Institute of Cell Biology, Chinese Academy of Sciences.
Lin
S Y
SY
Wang
Y H
YH
Zhao
D B
DB
D000818:Animals; D000911:Antibodies, Monoclonal; D004331:Drosophila melanogaster / Q000737:chemistry*; D019476:Insect Proteins / Q000302:isolation & purification*; D009866:Oogenesis
D000911:Antibodies, Monoclonal; D019476:Insect Proteins