[Detection and sequence analysis of the p53 gene mutation in epithelial ovarian cancer].
OBJECTIVE
To find the characteristics of p53 gene mutation in epithelial ovarian cancer and to analyze the relationship between p53 mutation and FIGO stage.


METHODS
p53 mutations in exon 5 to 7 were detected by single-strand conformational polymorphism (SSCP) and sequencing technique.


RESULTS
8 of 46 tumor tissues demonstrated a SSCP band shift in the region of the gene. All of them have been characterized to represent DNA alterations by sequencing, including 8 point mutations (6 missence, 1 silent mutation and 1 in intron) and a 1-base pair insertion (introducing a stop codon downstream). Overall, 88.9% of mutation were transitions, and most of them are G-->A transitions (7/8, 87.5%). 62.5% of the mutation were found in 175 and 245 codon. The percentage of the mutation in stage I and stage II was 20.0%, and in stage III and stage IV was 16.7% (P > 0.05).


CONCLUSIONS
The arising of p53 mutations in ovarian cancer is due to spontaneous error in DNA synthesis and repair. Codon 175, 245 are the two mutational hot spots. There is no relationship between the mutation of p53 gene and FIGO stage in epithelial ovarian cancer.
Guan
X
X
China-Japan Friendship Hospital, Beijing.
Lang
J
J
Bian
M
M
D000230:Adenocarcinoma / Q000235:genetics; D018269:Carcinoma, Endometrioid / Q000235:genetics*; D018284:Cystadenocarcinoma, Serous / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics*; D017354:Point Mutation; D017422:Sequence Analysis, DNA