Measurement of interleukin 10 in bronchoalveolar lavage from preterm ventilated infants.
BACKGROUND
Interleukin 10 (IL-10) is a cytokine that downregulates inflammation, in part by reducing the production of the proinflammatory cytokines IL-1beta and IL-8. It has been suggested that an inability to produce IL-10 might predispose preterm infants to develop chronic lung disease.


OBJECTIVE
To measure IL-10, IL-1beta, and IL-8 in bronchoalveolar lavage fluid from ventilated preterm infants in a prospective cohort study.


METHODS
17 consecutive newborn infants < or = 29 weeks' gestational age (median, 25; 9 boys) who were ventilated from birth underwent daily bronchoalveolar lavage sampling.


RESULTS
102 samples were collected, of which 57 contained IL-10 in amounts that were comparable with those found previously in ventilated term infants with respiratory failure. Chronic lung disease developed in 9 of the 11 survivors and all 9 infants had produced IL-10. IL-1beta and IL-8 were detected in nearly all samples and were raised throughout the course of sample collection.


CONCLUSIONS
IL-10 is readily detectable in early bronchoalveolar lavage samples from ventilated preterm infants, although it remains unclear whether this cytokine has any influence on the development of chronic lung disease.
McColm
J R
JR
Child Life and Health, Reproductive Medicine and Developmental Sciences, University of Edinburgh, 20 Sylvan Place, Edinburgh EH9 1UW, UK.
Stenson
B J
BJ
Biermasz
N
N
McIntosh
N
N
D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D005260:Female; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D016753:Interleukin-10 / Q000032:analysis*; D008173:Lung Diseases, Obstructive / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008297:Male; D011446:Prospective Studies; D012123:Pulmonary Ventilation; D012121:Respiration, Artificial
D015415:Biomarkers; D016753:Interleukin-10