Existence of multiple novel Gs alpha splice variants in acute leukemia patients.
The alpha subunit of the stimulatory G protein, Gs alpha, is involved in stimulation of the adenylate cyclase pathway of signal transduction. In this study, we investigated the status of the Gs alpha gene in 29 acute leukemia patients and identified three novel splice variants (designated Gs alpha L-1, Gs alpha L-2, and Gs alpha L-3), possibly derived from aberrant splicing. All of the splice variants have in-frame deletions, removing the functional domain responsible for GTPase activity of Gs alpha, and would encode truncated proteins of 160(Gs alpha L-1), 90(Gs alpha L-2) and 70(Gs alpha L-3) amino acids, respectively. The data suggest that these novel products may be implicated in an as-yet-unidentified signal transduction pathway in hematopoietic cells.
Ye
Q
Q
Molecular Genetics Center, Beijing Institute of Biotechnology, China. QY3x@virginia.edu
Lu
Q
Q
Zhang
S
S
Huang
J
J
Wang
H
H
Su
G
G
Huang
P
P
Huang
C
C
D000208:Acute Disease; D001483:Base Sequence; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012326:RNA Splicing; D017384:Sequence Deletion
D005819:Genetic Markers; D020033:Protein Isoforms; D019205:GTP-Binding Protein alpha Subunits, Gs