title: [A study of immobilized enzyme-assisted semisynthesis of human insulin].
abstract: In this paper, a study on preparation of human insulin by immobilized enzyme-assisted semisynthesis is reported for the first time in our country. Porcine insulin with Thr(Bu(t)OB(t) catalyzed by immobilized trypsin has been converted into human insulin via a two-step transpeptidation. With the systematic technique of optimization, the rate of transpeptidation is 66%-70%. PAGE photometry is used to determine the conversion rate of semisynthesis. PAGE and C-terminal analysis of protein are adopted to identify the semisynthesis human insulin.
authors.affiliation: Department of Biochemistry, Shool of Pharmacy.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D004800:Enzymes, Immobilized; D006801:Humans; D007328:Insulin / Q000096:biosynthesis*
chemical_list: D004800:Enzymes, Immobilized; D007328:Insulin