title: Cloning and nucleotide sequence of a gene encoding a glycogen debranching enzyme in the trehalose operon from Arthrobacter sp. Q36.
abstract: A gene located just upstream of the treYZ operon was isolated from Arthrobacter sp. strain Q36. The gene, designated treX, encoded an 823-amino acid protein. The amino acid sequence of the protein had 50% identity with the TreX protein (isoamylase) from Sulfolobus acidocaldarius ATCC 33909 which has a treZXY operon on the genome. We suggest that Arthrobacter treX is an isoamylase gene, and that it is a component of a treXYZ operon.
authors.affiliation: Hayashibara Biochemical Laboratories, Inc., 7-7 Amase-minami machi, Okayama, Japan. amaseken@hayahshibara.co.jp
authors.forename: K
authors.initials: K
authors.lastname: Maruta
authors.forename: M
authors.initials: M
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.forename: M
authors.initials: M
authors.lastname: Kurimoto
mesh_terms: D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006003:Glycogen / Q000378:metabolism; D006004:Glycogen Debranching Enzyme System / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon
chemical_list: D006004:Glycogen Debranching Enzyme System; D006003:Glycogen