title: A novel conformation of the herpes simplex virus origin of DNA replication recognized by the origin binding protein.
abstract: The Herpes simplex virus type I origin binding protein (OBP) is a sequence-specific DNA-binding protein and a dimeric DNA helicase encoded by the UL9 gene. It is required for the activation of the viral origin of DNA replication oriS. Here we demonstrate that the linear double-stranded form of oriS can be converted by heat treatment to a stable novel conformation referred to as oriS*. Studies using S1 nuclease suggest that oriS* consists of a central hairpin with an AT-rich sequence in the loop. Single-stranded oligonucleotides corresponding to the upper strand of oriS can adopt the same structure. OBP forms a stable complex with oriS*. We have identified structural features of oriS* recognized by OBP. The central oriS palindrome as well as sequences at the 5' side of the oriS palindrome were required for complex formation. Importantly, we found that mutations that have been shown to reduce oriS-dependent DNA replication also reduce the formation of the OBP-oriS* complex. We suggest that oriS* serves as an intermediate in the initiation of DNA replication providing the initiator protein with structural information for a selective and efficient assembly of the viral replication machinery.
authors.affiliation: Department of Medical Biochemistry, Göteborg University, Box 440, SE 405 30 Göteborg, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Aslani
authors.forename: S
authors.initials: S
authors.lastname: Simonsson
authors.forename: P
authors.initials: P
authors.lastname: Elias
mesh_terms: D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000378:metabolism; D010957:Plasmids; D018741:Replication Origin / Q000235:genetics*; D018139:Simplexvirus / Q000737:chemistry* / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D009841:Oligonucleotides; D014764:Viral Proteins; C064565:UL9 protein, Human herpesvirus 1; D015719:Single-Strand Specific DNA and RNA Endonucleases