[A study on interleukin-2 receptor genes in acute myeloid leukemia cells].
To investigate IL-2 receptor (IL-2R) gene expression in acute myeloid leukemia (AML) cells and the role of IL-2 in AML, the IL-2R alpha mRNA and IL-2R beta mRNA in leukemic cells obtained from bone marrow of 41 cases with AML before chemotherapy were measured by reverse transcriptase-polymerase chain reaction (RT-PCR). The responses to recombinant human IL-2 (rhIL-2) of these leukemic cells expressing IL-2R mRNA from 8 cases were assayed by 3H-thymidine incorporation. The IL-2R alpha mRNA and IL-2R beta mRNAs in leukemic cells were detected in 28 cases (68.3%) and 32 cases (78.0%), respectively. The IL-2R alpha mRNA and IL-2R beta mRNA in leukemic cells of 21 cases (51.2%) were expressed simultaneously. The responses to rhIL-2 of these leukemic cells from 8 cases were heterogeneous. The responses to rhIL-2 appeared in three types: proliferation, inhibition and no reaction. Only the cells of one case with M5 expressing IL-2R alpha mRNA and IL-2R beta mRNA responded proliferatively. The results demonstrate that the expressions of IL-2R genes in AML cells are a general phenomenon. The responses of these leukemic cells to rhIL-2 in vitro are heterogeneous.
Yang
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Department of Hematology, First Affiliated Hospital, Chengdu.
D015870:Gene Expression; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000235:genetics / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics / Q000378:metabolism; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; D015375:Receptors, Interleukin-2