title: Time-dependent changes of decorin in the infarct zone after experimentally induced myocardial infarction in rats: comparison with biglycan.
abstract: Decorin, a small dermatan sulphate proteoglycan, has been postulated to interact with other components of the extracellular matrix. We examined time-dependent changes of decorin in the infarct zone after experimentally induced myocardial infarction in rats by Northern blotting, in situ hybridization, and immunohistochemistry. The expression of decorin mRNA was compared to that of biglycan mRNA. Northern blotting demonstrated that the decorin mRNA expression was not increased in the infarct zone on day 2, while increased biglycan mRNA was observed at that time (average 3.1-fold increase). Decorin mRNA expression was increased on day 7, and reached a peak (average 2.2-fold increase) around day 14. Biglycan mRNA expression also reached a peak level around day 14 (average 13.3-fold increase). In situ hybridization revealed that mRNA signals for decorin did not appear in the infarct zone on day 2, while biglycan mRNA signals were observed. Decorin mRNA signals were observed in spindle-shaped mesenchymal cells in the infarct peripheral zone on day 7. The decorin mRNA signals appeared later than those of biglycan. Immunopositive staining for decorin was observed in the infarct zone on day 7. The present results demonstrated a time-dependent increase in decorin mRNA expression in mesenchymal cells in the infarct zone in rats. Decorin mRNA appeared later and was increased to a lower extent in the infarct zone than biglycan mRNA.
authors.affiliation: Department of Internal Medicine I, Faculty of Medicine, Okayama University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Doi
authors.forename: S
authors.initials: S
authors.lastname: Kusachi
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Nakahama
authors.forename: K
authors.initials: K
authors.lastname: Takeda
authors.forename: I
authors.initials: I
authors.lastname: Komatsubara
authors.forename: I
authors.initials: I
authors.lastname: Naito
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D058578:Biglycan; D015152:Blotting, Northern; D058575:Decorin; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins; D019084:Fluorescent Antibody Technique, Indirect; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D017403:In Situ Hybridization; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D058578:Biglycan; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger