Equine macrophage identification with an antibody (Ki-M6) to human CD68 and a new monoclonal antibody (JB10).
Monoclonal antibodies (mAbs) recognizing equine macrophages are scarce. The present study compared the immunocytochemical staining of various equine tissues (lymphoid tissue, lung, liver, small intestine, skin and blood leucocytes) by an antibody, Ki-M6, which detects CD68 in human macrophages and dendritic cells, and by a new anti-equine mAb, JB10, with staining produced by two previously described anti-equine macrophage mAbs, CZ2.2 and CZ3.3. Ki-M6 was shown to identify equine macrophages, which had a distribution different from those identified by CZ2.2 and CZ3.3. JB10 identified equine macrophages with a distribution similar to those identified by Ki-M6, but additionally bound to polymorphonuclear leucocytes. Flow cytometry of peripheral blood leucocyte subpopulations and tissue immunocytochemistry were used to compare staining by JB10 with that of CZ2.2 and CVS19; the latter identifies the myeloid antigen, EqCD13, found on polymorphonuclear leucocytes. The staining by JB10 differed from that of both CZ2.2 and CVS19, suggesting that JB10 detects a different molecule. These additional mAbs should prove useful for the future study of new, defined, populations of macrophages in equine immune responses and pathology, and, in the case of Ki-M6 antibody, may make possible an analysis of the structure, distribution and function of the CD68 molecule in the horse.
Siedek
E M
EM
Department of Pathology and Infectious Diseases, The Royal Veterinary College, Royal College Street, London, NW1 0TU, UK.
Honnah-Symns
N
N
Fincham
S C
SC
Mayall
S
S
Hamblin
A S
AS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000378:metabolism; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006736:Horses; D006801:Humans; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008198:Lymph Nodes / Q000737:chemistry; D008264:Macrophages / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D014018:Tissue Distribution; D000097382:CD68 Molecule
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C067980:CD68 antigen, human; D000097382:CD68 Molecule