title: Biliary tract reconstruction using an autologous vein graft in rats.
abstract: Numerous biologic and synthetic materials have been used with limited success as an interposed graft to repair segmental common bile duct (CBD) defects. The authors report here that an autologous vein graft can be successfully used to correct a CBD deficit contingent on accurate microsurgical technique immediate stenting and rapid graft vascularization. Thirty Sprague-Dawley rats underwent laparotomy and the experimental group (n=25) had a 3-mm segment of the CBD excised. The CBD defect was repaired using an interposed femoral vein graft aided by a plastic stent. The control group (n=5) had the CBD cut and repaired by means of primary anastomosis. The experimental group was subdivided into three sub-groups each examined at three different postoperative intervals: 1, 4 and 12 weeks. The results showed that inflammation was apparent in the venous wall following the first postoperative week. A progressive loss of the vascular endothelium and replacement with the columnar epithelium typical of the CBD was seen in the vein graft. Nineteen of the 25 experimental rats (76 percent) of the animals survived without complication from the surgery and there were no abnormalities in the liver biochemical tests of these animals. Any biliary tract obstruction that developed was attributed to dislocation of the stent leading to collapse of the vein graft (experimental group), or constriction of the anastomosis (control group). This study demonstrates that biliary tract reconstruction using an autologous vein graft can be successfully performed in a rat model of CBD repair. The application of this method to the clinical setting is also discussed.
authors.affiliation: Microsurgical Replantation Transplantation Service, Davies Medical Center, San Francisco, California, USA.
authors.forename: J Y
authors.initials: JY
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Moon
authors.forename: Z
authors.initials: Z
authors.lastname: Kryger
authors.forename: D Y
authors.initials: DY
authors.lastname: Shi
authors.forename: W C
authors.initials: WC
authors.lastname: Lineaweaver
authors.forename: H J
authors.initials: HJ
authors.lastname: Buncke
mesh_terms: D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000601:surgery; D003135:Common Bile Duct / Q000601:surgery*; D003137:Common Bile Duct Diseases / Q000601:surgery; D005268:Femoral Vein / Q000637:transplantation*; D008297:Male; D008866:Microsurgery / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015607:Stents; D013997:Time Factors; D014182:Transplantation, Autologous