Conjugated linoleic acid induces lipid peroxidation in humans.
Conjugated linoleic acid (CLA) is shown to have chemoprotective properties in various experimental cancer models. CLA is easily oxidised and it has been suggested that an increased lipid oxidation may contribute to the antitumorigenic effects. This report investigates the urinary levels of 8-iso-PGF(2alpha), a major isoprostane and 15-keto-dihydro-PGF(2alpha), a major metabolite of PGF(2alpha), as indicators of non-enzymatic and enzymatic lipid peroxidation after dietary supplementation of CLA in healthy human subjects for 3 months. A significant increase of both 8-iso-PGF(2alpha) and 15-keto-dihydro-PGF(2alpha) in urine was observed after 3 months of daily CLA intake (4.2 g/day) as compared to the control group (P<0.0001). Conjugated linoleic acid had no effect on the serum alpha-tocopherol levels. However, gamma-tocopherol levels in the serum increased significantly (P=0. 015) in the CLA-treated group. Thus, CLA may induce both non-enzymatic and enzymatic lipid peroxidation in vivo. Further studies of the mechanism behind, and the possible consequences of, the increased lipid peroxidation after CLA supplementation are urgently needed.
Basu
S
S
Section of Geriatrics/Clinical Nutrition Research, Faculty of Medicine, Uppsala University, Box 609, SE-751 25, Uppsala, Sweden. samar@basu@geriatrik.uu.se
Smedman
A
A
Vessby
B
B
D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D004311:Double-Blind Method; D028441:F2-Isoprostanes; D005260:Female; D006801:Humans; D007536:Isomerism; D008041:Linoleic Acids / Q000378:metabolism* / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D011863:Radioimmunoassay; D012016:Reference Values; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D028441:F2-Isoprostanes; D008041:Linoleic Acids; D014810:Vitamin E; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C075750:8-epi-prostaglandin F2alpha; D008315:Malondialdehyde; D015237:Dinoprost