title: Production of chimeric antibody in cells and larvae of silkworm.
abstract: By using the recombinant baculovirus expression system, the chimeric antibody against human small cell lung carcinoma was expressed in the cells and larvae of silkworm Bomyx mori N (BmN). The chimeric antibodies produced in BmN cells and larvae which were infected with recombinant viruses rNPVL2, rNPVH17 and dual-recombinant virus rNPVLH19 were detected by Western blot analysis. The co-expression products of heavy- and light-chain genes in silkworm larvae revealed stronger immune binding activity than the single product of heavy or light chain gene expression.
authors.affiliation: Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D018288:Carcinoma, Small Cell / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D007814:Larva; D008175:Lung Neoplasms / Q000276:immunology*; D017924:Nucleopolyhedroviruses / Q000235:genetics; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
chemical_list: D000906:Antibodies; D011993:Recombinant Fusion Proteins