[A study on pearl fat transplantation--concentration of basic fibroblast growth factor (bFGF) and carrier of sustained delivery selected by orthogonal design].
OBJECTIVE
The experiment was designed to solve the problem that free fat transplantation survived at low percentage.


METHODS
Based on physiochemical and biological characteristics of bFGF, fibrinogen was used as its carrier of sustained delivery. We applied bFGF and fibrinogen in pearl adipose transplantation. Different concentrations of bFGF and fibrinogen were used. The relation between the concentration of bFGF and fibrinogen and their effects on fat graft maintenance were observed.


RESULTS
At three months after transplantation, the weight of the fat graft varied from 98% to 225%. The latter was approximately consistent with synchronous increase of the body weight of the animal. Weight increase of the graft was closely related to the concentration of bFGF and the sustained delivery carrier. When bFGF in the concentration of 4000 U/10 microliters and fibrinogen of 1000 mg% were applied, the fat graft obtained the greatest weight increase. Histologically, the structure of the transplanted fat was normal with fibrotic envelope and mature adipocytes.


CONCLUSIONS
bFGF with fibrinogen as its carrier of sustained delivery favors the survival of fat graft and weight increase. The effects of bFGF and fibrinogen depend on their concentration.
Liao
T
T
Department of Stomatology, Hainan Province People's Hospital.
Xie
F
F
Wang
Q
Q
D000273:Adipose Tissue / Q000637:transplantation*; D000818:Animals; D003692:Delayed-Action Preparations; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D005340:Fibrinogen; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D006085:Graft Survival / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D016222:Fibroblast Growth Factor 2; D005340:Fibrinogen