[Culture of murine HPP-CFC in vitro using conditioned medium substitutefor recombinant growth factors].
OBJECTIVE
To study the clonal growth on single layer, agar of high proliferative potential colony-forming cells (HPP-CFC) of bone marrow obtained from both normal mice (NBM) and mice treated 2 days earlier with 5-fluorouracil (FU,BM) using conditioned media.


METHODS
Using liquid cell culture to harvert WEHI2-CM and L929-CM as the substitutes for IL-3 and M-CSF respectively; using semi-solid culture to assay HP-CFCin vitro.


RESULTS
(1) the combination of WEHI3-CM and L929-CM produced HPP-CFC in vitro, and the addition of rhIL-1 alpha, rhIL-6 and rmGM-CSF further enhanced HPP-CFC was enriched in the FU2 BM. Morphological investigation showed that the HPP-CFC colony was large and compact, great than 0.5mm in diameter and contained more than 50,000 cells, most of which were macrophage like cells. The hydroxyurea suicide rate for HPP-CFC was less than 10%.


CONCLUSIONS
The results suggested that (1) WEHI3-CM and L929-CM can be used as the substitutes of IL-3 and M-CSF to stimulate the formation of HPP-CFC; (2) FU can enrich HPP-CFC of the mice.
Xia
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Research Laboratory of Blood Physiology, Hunan Medical University, Changsha.
Jiang
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D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C
D017077:Culture Media, Conditioned; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005472:Fluorouracil