title: Bromocriptine-induced premature oestrus is associated with changes in the pulsatile secretion pattern of follicle-stimulating hormone in beagle bitches.
abstract: The secretory profiles of LH and FSH were investigated before and during the administration of bromocriptine in six beagle bitches. Plasma samples were obtained via jugular venepuncture at 10 min intervals for 6 h every 2 weeks until the next ovulation. Bromocriptine treatment was started 100 days after ovulation. Both before and after bromocriptine treatment, LH and FSH pulses occurred together. The mean duration of the FSH pulse (120 min) was significantly longer than that of the LH pulse (80 min). The interoestrous interval in the bitches treated with bromocriptine was significantly shorter than that of the preceding cycle (160 +/- 3 versus 206 +/- 24 days). The mean basal plasma FSH concentration (7.4 +/- 0.6 versus 6.1 +/- 0.7 iu l-1) and the mean area under the curve for FSH (46.6 +/- 4.7 versus 40.4 +/- 4.4 iu l-1 in 6 h) increased significantly after the start of the bromocriptine treatment. In contrast, the differences in mean basal plasma LH concentration (2.1 +/- 0.2 versus 2.0 +/- 0.2 micrograms l-1) and the mean area under the curve for LH (19.0 +/- 3.1 versus 19.5 +/- 2.5 micrograms l-1 in 6 h) between the day before and 14 days after the start of the bromocriptine treatment were not significant. Bromocriptine administration also lowered the mean amplitude of the FSH pulse and shortened the mean duration of the FSH pulse, without influencing the LH pulse. In addition to demonstrating the concurrent pulsatile secretion of LH and FSH, the results of the present study demonstrate that the bromocriptine-induced shortening of the interoestrous interval in the bitch is associated with an increase in plasma FSH concentration without a concomitant increase in plasma LH concentration. This finding indicates that treatment with the dopamine agonist bromocriptine increase plasma FSH to a concentration that results in the enhancement of follicle development.
authors.affiliation: Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
authors.forename: H S
authors.initials: HS
authors.lastname: Kooistra
authors.forename: A C
authors.initials: AC
authors.lastname: Okkens
authors.forename: M M
authors.initials: MM
authors.lastname: Bevers
authors.forename: C
authors.initials: C
authors.lastname: Popp-Snijders
authors.forename: B
authors.initials: B
authors.lastname: van Haaften
authors.forename: S J
authors.initials: SJ
authors.lastname: Dieleman
authors.forename: J
authors.initials: J
authors.lastname: Schoemaker
mesh_terms: D000704:Analysis of Variance; D000780:Anestrus / Q000187:drug effects*; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D004285:Dogs / Q000502:physiology*; D018491:Dopamine Agonists / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism; D011374:Progesterone / Q000097:blood; D018709:Statistics, Nonparametric
chemical_list: D018491:Dopamine Agonists; D001971:Bromocriptine; D011374:Progesterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone