title: Transplantation of cryopreserved human ovarian tissue results in follicle growth initiation in SCID mice.
abstract: OBJECTIVE
  To determine the long-term survival of frozen-thawed human ovarian tissue as xenografts in severe-combined-immunodeficiency (SCID) mice.
  
  
  METHODS
  Animal study.
  
  
  METHODS
  Animal and laboratory facilities at an academic center.
  
  
  METHODS
  Ovarian tissue obtained from a 27-year-old woman.
  
  
  METHODS
  Grafting of frozen-thawed ovarian tissue in SCID mice for 22 weeks.
  
  
  METHODS
  Follicle counts and growth by morphology and PCNA staining in frozen-thawed grafts and fresh controls.
  
  
  RESULTS
  All three grafts were recovered intact after 22 weeks. Their stroma was devoid of necrotic cells and contained healthy follicles. The ratio of primordial-total follicles decreased significantly after grafting (0.94 +/- 0.02 to 0.87 +/- 0. 01, control vs. grafting). Compared with controls, after 22 weeks of grafting, a higher percentage of follicles had initiated growth (5.6 +/- 2.4 vs. 12.5 +/- 1.9), but there was still a significant number of primordial follicles/graft (75 +/- 6.8). Follicle stages were similar between two groups; only primordial and one-layer follicles were seen in the xenografts. In the controls, except for one two-layer follicle, the most advanced follicle was at the one-layer stage.
  
  
  CONCLUSIONS
  Human primordial follicles survive freeze-thaw and long-term xenografting procedures and retain their capacity to initiate growth. These findings encourage future attempts for human autologous ovarian transplantation.
authors.affiliation: Cornell University-Weill Medical College and New York Methodist Hospital, New York, New York 11215, USA.
authors.forename: K
authors.initials: K
authors.lastname: Oktay
authors.forename: H
authors.initials: H
authors.lastname: Newton
authors.forename: R G
authors.initials: RG
authors.lastname: Gosden
mesh_terms: D000328:Adult; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000166:cytology* / Q000637:transplantation*; D014183:Transplantation, Heterologous