Characterisation of the proximal regulatory domain of the Echinococcus granulosus homeodomain-containing gene EgHbx1.
In an attempt to understand the molecular basis of the development of Echinococcus granulosus, we have previously isolated several homeobox-containing genes (EgHbx1-5). Here we report the characterisation of the EgHbx1 proximal regulatory domain. EgHbx1 codes for an amino acid sequence presenting 90% identity at the homeodomain level with the Drosophila melanogaster NK1-S59 transcription factor. The proximal regulatory domain sequence and the transcription start site were determined. Electrophoretic mobility shift assays using nuclear extracts from protoscoleces revealed the presence of specific DNA-protein complexes. These results constitute a hint and provide new tools to decipher regulatory cascades during E. granulosus development.
Esperón
P
P
Sección Bioquímica, Facultad de Ciencias, Iguá 4225, Montevideo, Uruguay.
Gorfinkiel
N
N
Garat
B
B
Ehrlich
R
R
D000818:Animals; D001483:Base Sequence; D004446:Echinococcus / Q000235:genetics*; D017238:Genes, Helminth; D005801:Genes, Homeobox; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic
D009687:Nuclear Proteins