title: A gene whose major transcript encodes only the substrate-binding domain of a protein-tyrosine kinase.
abstract: We have identified a novel protein-tyrosine kinase gene family in the simple multicellular animal Hydra vulgaris that consists of at least three members. Two of the genes encode receptor protein-tyrosine kinases. The third member of the family is unusual in that in non-sexual animals, the only transcripts that it produces encode polypeptides lacking all or nearly all of the ATP-binding lobe. Characterization of multiple cDNA clones and hybridization mapping of genomic DNA indicate that the gene, which we have termed Hinterteil (Hint), undergoes alternative cis-splicing, alternative trans-splicing, and alternative polyadenylation. In-situ hybridization analysis shows that expression of the gene is upregulated during spermatogenesis. Sexual males also produce an additional Hint transcript that is larger than the transcript seen in non-sexual animals, but still not large enough to encode a receptor.
authors.affiliation: Zoologisches Institut, Universität zu Köln, Cologne, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Kroiher
authors.forename: J C
authors.initials: JC
authors.lastname: Reidling
authors.forename: R E
authors.initials: RE
authors.lastname: Steele
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D020134:Catalytic Domain; D018076:DNA, Complementary; D015870:Gene Expression; D006829:Hydra; D008297:Male; D008969:Molecular Sequence Data; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018076:DNA, Complementary; C405403:Hinterteil protein, Hydra vulgaris; D012333:RNA, Messenger; D011061:Poly A; D011505:Protein-Tyrosine Kinases