title: Comparison of an oleic acid enriched-diet vs NCEP-I diet on LDL susceptibility to oxidative modifications.
abstract: OBJECTIVE
  The objective of this trial was to compare the effect on the susceptibility of plasma Low Density Lipoprotein (LDL) to oxidative modifications of consumption of two oleic rich diets, prepared with two different plant oils, virgin olive oil (OL)1 and refined high monounsaturated fatty acids (MUFA sunflower oil (SU)), with the susceptibility of plasma LDL to oxidation after an National Cholesterol Education Program step 1 (NCEP-I) phase diet.
  
  
  METHODS
  A randomized crossover design.
  
  
  METHODS
  Twenty-two healthy normolipidemic young males consumed an NCEP-I diet for a 4-week period. Subjects were then assigned to two diets each of 4-weeks duration. Group one was placed on an olive oil enriched diet (40% fat, 22% MUFA) followed by a 4-week period of a MUFA diet enriched in sunflower oil (40% fat, 22% MUFA). In group two, the order of the diets was reversed.
  
  
  RESULTS
  Both MUFA diets induced a decrease in saturated (14:0, 16:0, and 18:0) and an increase in monounsaturated and polyunsaturated n-6 (18:2, 20:3, and 20:5) plasma LDL-phospholipid fatty acids, compared to the NCEP-I diet (P<0.01). No significant differences in lag times were observed between the olive oil and the NCEP-I diet periods. However there was a greater inhibition time (P<0.001) when subjects consumed the MUFA rich sunflower oil diet compared to the NCEP-I diet. These differences were probably related to the relative enrichment of plasma LDL particles in alpha-tocopherol due to the high vitamin E content of the MUFA-rich sunflower oil. Indeed, the alpha-tocopherol content was positively correlated with lag time (r=0.338; P<0.008).
  
  
  CONCLUSIONS
  Our findings suggest that changes in plasma LDL alpha-tocopherol content with practical solid-food diets can decrease its susceptibility to oxidation.
  
  
  BACKGROUND
  This work has been supported by grants from the Investigaciones de la Seguridad Social (FIS 92/0182, to Francisco Pérez Jiménez); and from Koype Co, Andújar, Jaén, Spain. European Journal of Clinical Nutrition (2000) 54, 61-67
authors.affiliation: Lipids Research Unit, Hospital University Reina Sofia, Medical School, University of Córdoba, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Castro
authors.forename: J L
authors.initials: JL
authors.lastname: Miranda
authors.forename: P
authors.initials: P
authors.lastname: Gómez
authors.forename: D M
authors.initials: DM
authors.lastname: Escalante
authors.forename: F L
authors.initials: FL
authors.lastname: Segura
authors.forename: A
authors.initials: A
authors.lastname: Martín
authors.forename: F
authors.initials: F
authors.lastname: Fuentes
authors.forename: A
authors.initials: A
authors.lastname: Blanco
authors.forename: J M
authors.initials: JM
authors.lastname: Ordovás
authors.forename: F P
authors.initials: FP
authors.lastname: Jiménez
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015992:Body Mass Index; D018592:Cross-Over Studies; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D000069463:Olive Oil; D010084:Oxidation-Reduction / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology; D000074242:Sunflower Oil; D014810:Vitamin E / Q000097:blood
chemical_list: D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D008077:Lipoproteins, LDL; D000069463:Olive Oil; D010938:Plant Oils; D000074242:Sunflower Oil; D014810:Vitamin E