[A rapid simple and reliable method for sequencing the targeted gene of shuttle vector pSP189].
The authors established a simple, rapid and effective procedures for sequencing the targeted a-gene SupF TRNA of shuttle vector pSP189, which is very important and useful in molecular mechanism research of mutagens. In this method, double-stranded plasmid DNA as template, prepared single-stranded DNA with alkali denaturation, applied r-32P-ATP for labelling 5'-end of primer, were used. A satisfactory result was achieved.
Tan
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X
Institute of Food Safety Control and Inspection, Chinese Academy of Preventive Medicine, Beijing.
Li
S
S
Luo
X
X
D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D009152:Mutagenicity Tests; D017354:Point Mutation; D017422:Sequence Analysis, DNA