title: A peptide from the adenovirus fiber shaft forms amyloid-type fibrils.
abstract: The fiber protein of adenovirus consists of a C-terminal globular head, a shaft and a short N-terminal tail. The crystal structure of a stable domain comprising the head plus a part of the shaft of human adenovirus type 2 fiber has recently been solved at 2.4 A resolution [van Raaij et al. (1999) Nature 401, 935-938]. A peptide corresponding to the portion of the shaft immediately adjacent to the head (residues 355-396) has been synthesized chemically. The peptide failed to assemble correctly and instead formed amyloid-type fibrils as assessed by electron microscopy, Congo red binding and X-ray diffraction. Peptides corresponding to the fiber shaft could provide a model system to study mechanisms of amyloid fibril formation.
authors.affiliation: Department of Chemistry, San Francisco State University, 1600 Holloway avenue, 94132, San Francisco, CA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Luckey
authors.forename: J
authors.initials: J
authors.lastname: Hernandez
authors.forename: G
authors.initials: G
authors.lastname: Arlaud
authors.forename: V T
authors.initials: VT
authors.lastname: Forsyth
authors.forename: R W
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: A
authors.initials: A
authors.lastname: Mitraki
mesh_terms: D000260:Adenoviruses, Human / Q000737:chemistry*; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D001718:Birefringence; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins; D004396:Coloring Agents / Q000378:metabolism; D003224:Congo Red / Q000378:metabolism; D008854:Microscopy, Electron; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D014961:X-Ray Diffraction
chemical_list: D000682:Amyloid; D036022:Capsid Proteins; D004396:Coloring Agents; D010446:Peptide Fragments; C018315:hexon capsid protein, Adenovirus; D003224:Congo Red