title: Changes in leucine uptake in the retina of the hamster after traumatic detachment.
abstract: Protein metabolism was investigated in detached hamster retinas. By sucking off 0.2 ml of aqueous humor from the anterior chamber through limbic insertion of a 27-gauge needle, a tractional force pulled off the neural retina from the retinal pigment epithelium and created a simple detachment without retinal breaks in the right eyes of the hamsters. The left eyes were left untouched as normal controls and sham controls were induced by simple limbic insertion without suction. The animals were sacrificed at selected intervals of 1, 3, 6, 9, 16, 24, 32 days after the operation. Subsequently, scintillation counting and autoradiography were employed to study retinal protein metabolism using leucine uptake as an index. After tritiated leucine uptake, scintillation counting of radioactive substance indicated that detached retinas had taken in less tritiated leucine than normal controls from day 1 to 6 after the operation, but this change had normalized by day 9. For autoradiography, the change in leucine uptake rate was shown to be different in different layers. All the retinal cells seemed to show a decreased leucine uptake with the exception of the outer nuclear layer, in which leucine appeared to be significantly upregulated. This paper illustrates the patterns of protein metabolism and their change after traumatic detachment as well as their possible recovery.
authors.affiliation: Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
authors.forename: K
authors.initials: K
authors.lastname: Yau
authors.forename: W W
authors.initials: WW
authors.lastname: Li
authors.forename: D T
authors.initials: DT
authors.lastname: Yew
mesh_terms: D000818:Animals; D001345:Autoradiography; D001693:Biological Transport, Active; D006224:Cricetinae; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D007930:Leucine / Q000378:metabolism*; D008647:Mesocricetus; D012160:Retina / Q000378:metabolism; D012163:Retinal Detachment / Q000378:metabolism* / Q000473:pathology; D012588:Scintillation Counting; D013997:Time Factors; D014316:Tritium
chemical_list: D005136:Eye Proteins; D014316:Tritium; D007930:Leucine