[Effect of serotype specific glycopeptidolipid (GPL) isolated from Mycobacterium avium complex (MAC) on phagocytosis and phagosome-lysosome fusion of human peripheral blood monocytes].
Mycobacterium avium complex (MAC) is a typical intracellular parasite similar to M. tuberculosis and is one of the most important pathogens that coinfects AIDS patients. Attention has been focused on M. avium infection causing immunosuppression of hosts. Specific serotype-subspecies such as 1, -4 or -8 serotypes can be isolated frequently in humans infected with HIV. Furthermore, the prognosis after infection differs depending on the serotype. Serotype-4 in general shows unfavourable prognosis, while serotype-16 yields rapid recovery. Therefore, we have been interested in the immunomodifying activity of the surface glycopeptidolipid (GPL) antigen. However, no information has been available to date dealing on the virulent factor of MAC that is directly related with intracellular bactericidal activity. Recently, we have tried to test the effect of various GPLs purified from MAC on phagocytic processes of human peripheral blood monocytes (PBMC). We have used GPL-coated heat-killed staphylococcal cells to be phagocytosed by PBMC, and phagosome-lysosome fusion (P-L fusion) was estimated by the acridine orange staining of fused vesicles and bacteria. Results showed strong promotion of phagocytosis and marked inhibition of P-L fusion by serotype-4 GPL, while neither promotion of phagocytosis nor inhibition of P-L fusion in phagocytic cells were shown by serotype-16 GPL. Serotype-8 GPL showed concomitant stimulation of both phagocytosis and P-L fusion. These effects may be due to some unknown interaction between specific carbohydrate chain and organella membranes and serotype-4 GPL may be one of the possible virulent factors in MAC. Comparison with known possible virulent factors such as trehalose 6,6'-dimycolate (TDM), trehalose 6-monomycolate (TMM), glucose 6-monomycolate (GM) or sulfatide was also reported.
Takegaki
Y
Y
Department of Bacteriology and Urology, Osaka City University Medical School, Japan.
D002478:Cells, Cultured; D006017:Glycolipids / Q000302:isolation & purification / Q000494:pharmacology*; D006020:Glycopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008247:Lysosomes / Q000502:physiology; D008561:Membrane Fusion / Q000187:drug effects*; D009000:Monocytes / Q000276:immunology*; D015269:Mycobacterium avium Complex / Q000737:chemistry / Q000145:classification* / Q000472:pathogenicity; D010587:Phagocytosis / Q000187:drug effects*; D010588:Phagosomes / Q000502:physiology; D012703:Serotyping; D014774:Virulence
D006017:Glycolipids; D006020:Glycopeptides