title: Changes in peritoneal membrane after continuous ambulatory peritoneal dialysis--a histopathological study.
abstract: Peritoneal membrane changes in continuous ambulatory peritoneal dialysis (CAPD) patients have been widely described but poorly classified. Our aim was to identify the morphological changes occurring after CAPD treatment. To this end, 17 biopsies of parietal peritoneum (1 cm in diameter) were withdrawn at least 5 cm from the catheter entry hole and stained with Van Gieson, hematoxylin-eosin, trichrome, and some immunohistochemical stains: keratin, vimentin, CD34, CD20, CD4, CD8, desmin, and collagen IV. The morphology of mesothelium, vessels, and basement membrane (BM) of mesothelium and vessels, the presence of inflammatory cells, fibrin, and calcifications, and the distribution and thickness of submesothelial tissue were evaluated. Patients were divided into three groups according to the thickness of the sclerotic band replacing mesothelium: group 1, band up to 40 microns; group 2, band less than 40 microns; group 3, no sclerotic band. The main histopathological alterations noted were: loss of mesothelium; sclerotic alteration of vessels or duplication of BM; presence of myofibroblasts; and presence of inflammatory cells (sparse, focal, or perivascular), mainly represented by macrophages and CD4+ lymphocytes. No significant qualitative differences were observed between the three groups. In conclusion, the variable histological changes in peritoneal membrane suggest a routine peritoneal biopsy in any surgical procedure to better understand pathological changes in the course of CAPD treatment.
authors.affiliation: Renal Unit, Sacco Hospital, Milan, Italy.
authors.forename: S V
authors.initials: SV
authors.lastname: Bertoli
authors.forename: M T
authors.initials: MT
authors.lastname: Barone
authors.forename: L
authors.initials: L
authors.lastname: Vago
authors.forename: S
authors.initials: S
authors.lastname: Bonetto
authors.forename: A
authors.initials: A
authors.lastname: De Vecchi
authors.forename: A
authors.initials: A
authors.lastname: Scalamogna
authors.forename: G
authors.initials: G
authors.lastname: Barbiano di Belgiojoso
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001485:Basement Membrane / Q000473:pathology; D015704:CD4 Antigens / Q000032:analysis; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects; D010537:Peritoneum / Q000473:pathology*; D012598:Sclerosis
chemical_list: D015704:CD4 Antigens