ELISA for a complexed antigen with a monoclonal antibody blocking reaction with the free antigen-assay-specific for complexed prostate-specific antigen.
We have developed an enzyme-linked immunoassay (ELISA) for serum complexed-antigen (prostate-specific antigen; PSA)(c-PSA) with simultaneous blocking of free-antigen (PSA)(f-PSA). The assay utilizes three different monoclonal antibodies (MAbs) recognising three distinct PSA epitopes. The detection limit was established as 0.19 microg/l (n=20, mean of zero standard+2S.D.) and the average recovery of f-PSA was 98-100%. The within-run and between-day coefficients of variation (CV) ranged from 2.1% to 3.2% and 2.8% to 6.3%, respectively. There was a good correlation between serum c-PSA measured by the present ELISA and PSA-alpha(1)-antichymotrypsin complex (PSA-ACT) concentrations (r=0.991). This method should provide a better tool for discriminating between benign and malignant prostatic disease.
Matsumoto
K
K
Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Japan.
Konishi
N
N
Samori
T
T
Kimura
E
E
Doi
M
M
Kato
S
S
Yuki
Y
Y
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016014:Linear Models; D008297:Male; D017430:Prostate-Specific Antigen / Q000097:blood* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000514:alpha 1-Antichymotrypsin / Q000276:immunology
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000514:alpha 1-Antichymotrypsin; D017430:Prostate-Specific Antigen