title: From long range mapping to sequence-ready contigs on human chromosome 6.
abstract: Our aim is to construct physical clone maps covering those regions of chromosome 6 that are not currently extensively mapped, and use these to determine the DNA sequence of the whole chromosome. The strategy we are following involves establishing a high density framework map of the order of 15 markers per Megabase using radiation hybrid (RH) mapping. The markers are then used to identify large-insert genomic bacterial clones covering the chromosome, which are assembled into sequence-ready contigs by restriction enzyme fingerprinting and sequence tagged site (STS) content analysis. Contig gap closure is performed by walking experiments using STSs developed from the end sequences of the clone inserts.
authors.affiliation: The Sanger Centre, Hinxton, Cambridge, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Mungall
authors.forename: S J
authors.initials: SJ
authors.lastname: Humphray
authors.forename: S A
authors.initials: SA
authors.lastname: Ranby
authors.forename: C A
authors.initials: CA
authors.lastname: Edwards
authors.forename: R W
authors.initials: RW
authors.lastname: Heathcott
authors.forename: C M
authors.initials: CM
authors.lastname: Clee
authors.forename: E
authors.initials: E
authors.lastname: Holloway
authors.forename: A I
authors.initials: AI
authors.lastname: Peck
authors.forename: P
authors.initials: P
authors.lastname: Harrison
authors.forename: L D
authors.initials: LD
authors.lastname: Green
authors.forename: A P
authors.initials: AP
authors.lastname: Butler
authors.forename: C F
authors.initials: CF
authors.lastname: Langford
authors.forename: R G
authors.initials: RG
authors.lastname: William
authors.forename: E J
authors.initials: EJ
authors.lastname: Huckle
authors.forename: L
authors.initials: L
authors.lastname: Baron
authors.forename: A
authors.initials: A
authors.lastname: Smith
authors.forename: M A
authors.initials: MA
authors.lastname: Leversha
authors.forename: Y H
authors.initials: YH
authors.lastname: Ramsey
authors.forename: S M
authors.initials: SM
authors.lastname: Clegg
authors.forename: C M
authors.initials: CM
authors.lastname: Rice
authors.forename: G L
authors.initials: GL
authors.lastname: Maslen
authors.forename: S E
authors.initials: SE
authors.lastname: Hunt
authors.forename: C E
authors.initials: CE
authors.lastname: Scott
authors.forename: C A
authors.initials: CA
authors.lastname: Soderlund
authors.forename: I
authors.initials: I
authors.lastname: Dunham
mesh_terms: D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D020451:Contig Mapping; D016208:Databases, Factual; D006801:Humans; D017422:Sequence Analysis, DNA