title: Cloning and characterization of the ribosomal protein CcP0 of the medfly Ceratitis capitata.
abstract: The gene of the ribosomal protein CcP0, the third member of the ribosomal P-protein family of the medfly Ceratitis capitata, was identified by genomic and cDNA sequence analysis. It codes for a polypeptide of 317 amino acids and its predicted amino acid sequence shows great similarity to the P0 proteins of other eukaryotic organisms. The CcP0 gene was expressed in Escherichia coli and the 34-kDa recombinant protein was identical to the P0 protein of purified medfly ribosomes. Both proteins reacted positively with a specific monoclonal antibody against the highly conserved C terminus of eukaryotic ribosomal P proteins. Interestingly, the medfly CcP0 seems to be the only P0 protein of higher eukaryotic organisms with basic character (pI 8.5), as shown by electrofocusing of purified ribosomes.
authors.affiliation: University of Athens, Department of Biology, Division of Genetics and Biotechnology, Panepistimiopolis, Athens, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Gagou
authors.forename: J P
authors.initials: JP
authors.lastname: Ballesta
authors.forename: S
authors.initials: S
authors.lastname: Kouyanou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004175:Diptera / Q000235:genetics*; D004331:Drosophila melanogaster; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae
chemical_list: D010750:Phosphoproteins; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C054675:ribosomal protein P0