title: Lack of association between Streptococcus oralis and recurrent aphthous stomatitis.
abstract: In the present study, the potential involvement of Streptococcus oralis in the aetiology of recurrent aphthous stomatitis (RAS) was investigated using the polymerase chain reaction (PCR). Biopsies from 28 RAS patients were analysed, in addition to 20 oral lichen planus (OLP) and 13 normal biopsies that were used as controls. PCR was carried out using a primer pair that targets the D-alanine:D-alanine ligase gene and detects DNA from both S. oralis and the closely related species Streptococcus mitis. Discrimination between these two species was achieved by digestion of PCR products with the restriction endonucleases HaeIII and HindIII, which both give distinct restriction profiles for each species. S. oralis DNA was detected in 8 of 28 (29%) RAS samples, 10 of 20 (50%) OLP samples and 6 of 13 (46%) normal samples. These results suggest that S. oralis is not of primary aetiological significance in RAS.
authors.affiliation: Infection Research Group, University of Glasgow Dental School, UK.
authors.forename: M P
authors.initials: MP
authors.lastname: Riggio
authors.forename: A
authors.initials: A
authors.lastname: Lennon
authors.forename: F
authors.initials: F
authors.lastname: Ghodratnama
authors.forename: D
authors.initials: D
authors.lastname: Wray
mesh_terms: D000328:Adult; D001483:Base Sequence; D001706:Biopsy; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D017676:Lichen Planus, Oral / Q000382:microbiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000382:microbiology / Q000473:pathology; D016133:Polymerase Chain Reaction / Q000379:methods; D012008:Recurrence; D015183:Restriction Mapping / Q000379:methods; D013281:Stomatitis, Aphthous / Q000382:microbiology* / Q000473:pathology; D019533:Streptococcus oralis / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial