Development of human oocytes matured in vitro for 28 or 36 hours.
OBJECTIVE
To compare the effects on human oocytes of in vitro maturation periods of 28 hours and 36 hours.


METHODS
Retrospective analysis.


METHODS
University teaching hospital.


METHODS
A total of 48 infertile patients undergoing 55 cycles who volunteered for the experimental treatments.


METHODS
Immature oocytes were aspirated with use of transvaginal ultrasonography. Oocytes were matured, fertilized by intracytoplasmic sperm injection, and cultured for 2.5 or 3 days before being replaced into the uterus.


METHODS
Oocyte maturation, fertilization and cleavage rates, and clinical pregnancy rate.


RESULTS
Maturation of oocytes for either 28 hours or 36 hours before insemination resulted in similar rates of maturation, fertilization, and cleavage. The clinical pregnancy rate was similar in both groups (14%-15%).


CONCLUSIONS
Shortening the in vitro maturation period does not compromise subsequent embryonic development.
Smith
S D
SD
Fertility Clinic, Herlev University Hospital, Herlev, Denmark.
Mikkelsen
A
A
Lindenberg
S
S
D048429:Cell Size; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007247:Infertility, Female / Q000628:therapy; D008677:Metaphase; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D013997:Time Factors; D016896:Treatment Outcome