Affinity adsorbents for the vancomycin group of antibiotics.
The vancomycin group of antibiotics kill Gram-positive bacteria by binding to nascent bacterial cell-wall peptidoglycan bearing the C-terminal sequence-D-Ala-D-Ala. In this paper, affinity adsorbents for the vancomycin group of antibiotics were prepared by immobilizing the peptidoglycan analogues -D-Ala-D-Ala, -succinyl-D-Ala and -succinyl-Gly on to crosslinked poly(N, N-dimethylacrylamide). The adsorption capacities of the three adsorbents for demethylvancomycin were 0.59, 0.35 and 0.29 mmol/g, respectively. The adsorption capacity of the adsorbent with-D-Ala-D-Ala for vancomycin was 0.53 mmol/g. In contrast, the adsorbent bearing -succinyl-L-Ala hardly adsorbed demethylvancomycin. Aqueous sodium carbonate (0.4 M)/acetonitrile (7/3, v/v) completely desorbed demethylvancomycin adsorbed on the adsorbents.
Yan
H
H
The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, People's Republic of China.
Zhao
Q
Q
Yuan
J
J
Cheng
X
X
He
B
B
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002473:Cell Wall / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry* / Q000378:metabolism; D014640:Vancomycin / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D010455:Peptides; D010457:Peptidoglycan; D014640:Vancomycin; C041572:N-demethylvancomycin