[Application of serum bile acid chromatography to the diagnoses of liver diseases].
In order to explore the specificity of serum bile acid (SBA) chromatograph in the diagnoses of different kinds of hepatosis, we investigated by means of gas chromatography the changes of serum bile acids in workers who exposed to hexogen or chloroethylene and in patients who suffered from hepatosis such as acute jaundice hepatitis, chronic active hepatitis, cirrhosis and liver cancer. The results revealed different disturbances of SBA occurring in the liver injuries induced by the two kinds of hepatotoxicant. Serum lithocholic acid (LCA), deoxycholic acid (DCA) and chenodeoxycholic acid (CDCA) in workers exposed to hexogen and wre significantly different from those of the control group respectively (P < 0.01, P < 0.01, P < 0.05); on the other hand, only serum LCA and DCA went up in workers exposed to chloroethylene (P < 0.0005, P < 0.001). The main changes both concentrated on the secondary bile acids. In acute jaundice hepatitis, chronic active hepatitis, cirrhosis and liver cancer, serum LCA, DCA, CDCA and cholic acids (CA) all went up in different degrees compared with the control group respectively (P < 0.005, P < 0.025, P < 0.005, P < 0.005). But no difference was noted among the 4 kinds of bile acids (P > 0.5), except that between CA and CDCA. These provided the evidence of the diagnosis and identification of clinical hepato-biliary diseases and occupational liver injures.
Dong
Y
Y
Analytical Test Center, Chengdu.
Li
J
J
Xiang
S
S
Yang
S
S
Bao
Z
Z
Fan
H
H
Yang
F
F
Li
Z
Z
D002793:Cholic Acids / Q000097:blood*; D002849:Chromatography, Gas; D003937:Diagnosis, Differential; D006801:Humans; D008107:Liver Diseases / Q000175:diagnosis*; D011041:Poisoning / Q000097:blood; D014227:Triazines / Q000506:poisoning; D014752:Vinyl Chloride / Q000506:poisoning
D002793:Cholic Acids; D014227:Triazines; C009160:cyclonite; D014752:Vinyl Chloride