Improved method for non-denaturing polyacrylamide gradient gel electrophoresis for detection of small-sized LDL produced during postprandial hypertriglyceridaemia.
A reliable method for detection of small-sized low-density lipoprotein (LDL) produced during transient hypertriglyceridaemia induced by ingestion of fat is described. Electrophoresis using a commercial non-denaturing 2.0-16% polyacrylamide gradient gel is commonly utilized to determine the size of LDL particles, but it failed to detect a minor change in LDL size induced during postprandial hypertriglyceridaemia. Detection of small-sized LDL was achieved by adjusting the polyacrylamide concentration. Electrophoresis using a non-denaturing 1.5-10% polyacrylamide gradient gel gave the best resolution for detecting small-sized LDL induced during postprandial hypertriglyceridaemia. This improved method may be useful for elucidating the underlying mechanism of production of small-sized LDL (small dense LDL) in subjects with chronic hypertriglyceridaemia.
Ashida
Y
Y
Department of Etiology and Pathophysiology, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
Takagi
A
A
Ikeda
Y
Y
D004041:Dietary Fats / Q000008:administration & dosage; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D015228:Hypertriglyceridemia / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood*; D010316:Particle Size; D019518:Postprandial Period; D013997:Time Factors
D004041:Dietary Fats; D008077:Lipoproteins, LDL