Effect of retinoic acid on osteopontin expression in rat clonal dental pulp cells.
We studied the effect of retinoic acid on osteopontin synthesis and the mRNA expression in rat clonal dental pulp cells, RPC-C2A. An immunoprecipitation assay clarified that retinoic acid caused an increase in phosphorylated osteopontin synthesis that was dose-dependent, and marked increases were observed at retinoic acid concentrations of 10(-6) to 10(-5) M (1.7-fold). A Northern blotting analysis revealed a similar pattern of increase in osteopontin mRNA expression of up to 6.2-fold of control levels. Because osteopontin has an important role in the mineralization process, these results suggest that retinoic acid regulates mineralization, which takes place in the pulp cavity, including reparative dentin formation.
Ohishi
M
M
Department of Periodontology and Endodontology, Tokushima University School of Dentistry, Japan.
Horibe
M
M
Ikedo
D
D
Miyazaki
M
M
Ohishi
K
K
Kataoka
M
M
Kido
J
J
Nagata
T
T
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D053495:Osteopontin; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012795:Sialoglycoproteins / Q000096:biosynthesis*; D014074:Tooth Calcification / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*
D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin; D014212:Tretinoin