Nucleotide sequence of the gene encoding murine tissue factor pathway inhibitor-2.
Tissue factor pathway inhibitor-2 (TFPI-2), also known as placental protein 5, is a 32 kDa extracellular matrix-associated serine proteinase inhibitor consisting of three tandemly-arranged Kunitz-type domains. Two overlapping genomic clones containing sequences encoding murine TFPI-2 were isolated from a lambda FIXII 129 SVJ mouse genomic library, and the complete nucleotide sequence of the gene was determined. The murine TFPI-2 gene spans approximately 9.3 kilobases and consists of five exons and four introns. The nucleotide sequences surrounding all the exon-intron boundaries are highly conserved and obey the GT-AG rule. Each Kunitz-type domain is encoded by a single exon, similar to that observed for other Kunitz-type proteinase inhibitors. A total of 1,577 bp of the 3'-flanking region contains a probable polyadenylation site (ATTAAA) at +5,759 and an apparent cleavage or termination site (CATTG) at +6,170. The 5'-flanking region of the murine TFPI-2 gene contains a prototypical TATA box, a GC box and two CAAT boxes. In addition, several candidate transcription factor binding sites responsible for placenta-, endothelial cell-, and smooth muscle cell-specific expression of the TFPI-2 gene were also identified.
Kazama
Y
Y
Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque 87131-5301, USA.
Kamei
S
S
Kuijper
J L
JL
Foster
D C
DC
Kisiel
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016678:Genome; D006023:Glycoproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics
D006023:Glycoproteins; D011257:Pregnancy Proteins; D014157:Transcription Factors; C021240:tissue-factor-pathway inhibitor 2