Production of chimeric antibody in cells and larvae of silkworm.
By using the recombinant baculovirus expression system, the chimeric antibody against human small cell lung carcinoma was expressed in the cells and larvae of silkworm Bomyx mori N (BmN). The chimeric antibodies produced in BmN cells and larvae which were infected with recombinant viruses rNPVL2, rNPVH17 and dual-recombinant virus rNPVLH19 were detected by Western blot analysis. The co-expression products of heavy- and light-chain genes in silkworm larvae revealed stronger immune binding activity than the single product of heavy or light chain gene expression.
Yang
G
G
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
Wu
X
X
Liu
J
J
Zhang
Z
Z
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D018288:Carcinoma, Small Cell / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D007814:Larva; D008175:Lung Neoplasms / Q000276:immunology*; D017924:Nucleopolyhedroviruses / Q000235:genetics; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
D000906:Antibodies; D011993:Recombinant Fusion Proteins