Characterization of a carboxypeptidase A gene from the mosquito, Aedes aegypti.
A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late ( approximately 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.
Edwards
M J
MJ
Ohio Wesleyan University, Delaware, OH, USA.
Moskalyk
L A
LA
Donelly-Doman
M
M
Vlaskova
M
M
Noriega
F G
FG
Walker
V K
VK
Jacobs-Lorena
M
M
D000330:Aedes / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002268:Carboxypeptidases / Q000235:genetics*; D043422:Carboxypeptidases A; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis
D012333:RNA, Messenger; D002268:Carboxypeptidases; D043422:Carboxypeptidases A