title: Deficiency of a STE20/PAK family kinase LOK leads to the acceleration of LFA-1 clustering and cell adhesion of activated lymphocytes.
abstract: Lymphocyte-oriented kinase (LOK) is a member of the STE20/p21-activated kinase (PAK) family and expressed predominantly in lymphoid organs. Generation of LOK-deficient mice revealed that the leukocyte-function-associated antigen (LFA-1)/intercellular adhesion molecules (ICAM)-mediated aggregation of mitogen-stimulated T cells was greatly enhanced in the absence of LOK. Though levels of total LFA-1 and ICAMs as well as the active form of LFA-1 on T cell blasts were comparable in the presence and absence of LOK, clustering of active LFA-1 detected by binding of soluble ICAM-1 was accelerated in the absence of LOK. These results suggest that LOK is potentially involved in the regulation of LFA-1-mediated lymphocyte adhesion.
authors.affiliation: Department of Immunology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Endo
authors.forename: N
authors.initials: N
authors.lastname: Toyama-Sorimachi
authors.forename: C
authors.initials: C
authors.lastname: Taya
authors.forename: S
authors.initials: S
authors.lastname: Kuramochi-Miyagawa
authors.forename: K
authors.initials: K
authors.lastname: Nagata
authors.forename: K
authors.initials: K
authors.lastname: Kuida
authors.forename: T
authors.initials: T
authors.lastname: Takashi
authors.forename: H
authors.initials: H
authors.lastname: Yonekawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshizawa
authors.forename: N
authors.initials: N
authors.lastname: Miyasaka
authors.forename: H
authors.initials: H
authors.lastname: Karasuyama
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A; D015698:Genomic Library; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D003208:Concanavalin A; D018799:Intercellular Adhesion Molecule-1; D017346:Protein Serine-Threonine Kinases; C108145:Stk10 protein, mouse