title: Ganglioside expression during differentiation of chick retinal cells in vitro.
abstract: The neural retina has been widely used to study the developmental patterns of ganglioside metabolism. Recent findings about in vitro differentiating chick embryo retina cells showed that: a) GD3 and GD1a ganglioside patterns undergo the most dramatic changes; b) when the cells emit neurites, GD3 ganglioside and a group of complex gangliotetraosylgangliosides (GTOG) are transiently coexpressed; c) synchronized developmental phenomena are dissociated by anti-GM1 antibodies; d) GD3 remains as a major ganglioside in differentiated neurons, though it is almost not immunoexpressed; e) GTOG affect antibody binding to GD3; f) the content of gangliosides involved in neural differentiation modifies their immunostain localization on cell membrane; g) after exogenous GTOG uptake, immature neurons mimic GD3 immunofluorescent localization of mature cells; h) a subset of purified retinal ganglion cells express GTOG characteristic of mature neurons.
authors.affiliation: Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC, UNC-CONICET-Departamento de Química Biológica Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. panzetta@dqbfcq.uncor.edu
authors.forename: P
authors.initials: P
authors.lastname: Panzetta
authors.forename: M L
authors.initials: ML
authors.lastname: Allende
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D005732:Gangliosides / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000196:embryology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D005732:Gangliosides