Crystallization and preliminary X-ray analysis of Thermotoga maritima ribosome recycling factor.
Thermotoga maritima ribosome recycling factor (RRF) is one of the proteins catalyzing the fourth step in prokaryotic protein synthesis, ribosome recycling. The RRF protein was crystallized with ammonium sulfate. Native diffraction data to 2.55 A resolution were obtained at the MAX II synchrotron from a flash-frozen crystal at 100 K. The crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 47, c = 298 A, and probably contain one monomer per asymmetric unit.
Selmer
M
M
Department of Molecular Biophysics, Center for Chemistry and Chemical Engineering, Lund University, Box 124, 22100 Lund, Sweden.
Al-Karadaghi
S
S
Hirokawa
G
G
Kaji
A
A
Liljas
A
A
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000378:metabolism; D020124:Thermotoga maritima / Q000737:chemistry* / Q000378:metabolism
D001426:Bacterial Proteins; D011506:Proteins; D012269:Ribosomal Proteins; C087267:ribosome releasing factor