title: Helicobacter pylori water-soluble surface proteins activate human neutrophils and up-regulate expression of CXC chemokines.
abstract: To elucidate the mechanisms of the persistent neutrophil recruitment in H. pylori-infected gastric mucosa, we evaluated the activation of human neutrophils and CXC chemokine expression in neutrophils by H. pylori water-soluble surface proteins. H. pylori water extract (HPWE) was prepared from a supernatant of the H. pylori suspension in distilled water. After neutrophils were stimulated with HPWE, the mobilization of intracellular free calcium, the expression of lymphocyte function-associated antigen-1beta, and the secretion of myeloperoxidase (MPO) were enhanced in the neutrophils. In H. pylori-infected gastric mucosa, transendothelial and transepithelial migration of neutrophils were observed by electron microscopy and mucosal MPO levels were elevated. Up-regulation of the expression of interleukin-8 (IL-8) and growth-related oncogenes (GROs; GROalpha, GRObeta and GROgamma) mRNA and protein in neutrophils by HPWE was demonstrated by quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. In conclusion, H. pylori-induced neutrophil recruitment may be mediated by CXC chemokines which are expressed by neutrophils activated by H. pylori water-soluble surface proteins.
authors.affiliation: Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Chongno-gu, Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.forename: H C
authors.initials: HC
authors.lastname: Jung
authors.forename: J M
authors.initials: JM
authors.lastname: Kim
authors.forename: I S
authors.initials: IS
authors.lastname: Song
authors.forename: C Y
authors.initials: CY
authors.lastname: Kim
mesh_terms: D019743:Chemokines, CXC / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008875:Middle Aged; D018375:Neutrophil Activation / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D014510:Urease / Q000494:pharmacology
chemical_list: D019743:Chemokines, CXC; D014510:Urease