title: Mitochondrial DNA variation of Triatoma infestans populations and its implication on the specific status of T. melanosoma.
abstract: DNA sequence comparison of 412 base-pairs fragments of the mitochondrial cytochrome B gene was used to infer the genetic structure of nine geographical Triatoma infestans populations and their phylogenetic relationship with T. melanosoma and T. brasiliensis. T. infestans and T. melanosoma were compared by morphometry, allozyme and cytogenetic analyses, as well as subjected to reciprocal crosses, in order to clarify the taxonomic status of the latter. No differences were found to distinguish the two species and the crosses between them yielded progeny. T. infestans populations presented four haplotypes that could be separated in two clusters: one formed by the samples from Bolivia (Andes and Chaco) and the other formed by samples from Argentina and Brazil. Silvatic and domestic T. infestans populations from Bolivia (Andes) were genetically identical.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, GA, USA. fdm8@cdc.gov
authors.forename: F A
authors.initials: FA
authors.lastname: Monteiro
authors.forename: R
authors.initials: R
authors.lastname: Pérez
authors.forename: F
authors.initials: F
authors.lastname: Panzera
authors.forename: J P
authors.initials: JP
authors.lastname: Dujardin
authors.forename: C
authors.initials: C
authors.lastname: Galvão
authors.forename: D
authors.initials: D
authors.lastname: Rocha
authors.forename: F
authors.initials: F
authors.lastname: Noireau
authors.forename: C
authors.initials: C
authors.lastname: Schofield
authors.forename: C B
authors.initials: CB
authors.lastname: Beard
mesh_terms: D000818:Animals; D001118:Argentina; D001483:Base Sequence; D001838:Bolivia; D001938:Brazil; D003573:Cytochrome b Group / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry* / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry* / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014224:Triatoma / Q000166:cytology / Q000235:genetics*
chemical_list: D003573:Cytochrome b Group; D004272:DNA, Mitochondrial; D016054:DNA, Protozoan