A family of ubiquitin-like proteins binds the ATPase domain of Hsp70-like Stch.
We have isolated two human ubiquitin-like (UbL) proteins that bind to a short peptide within the ATPase domain of the Hsp70-like Stch protein. Chap1 is a duplicated homologue of the yeast Dsk2 gene that is required for transit through the G2/M phase of the cell cycle and expression of the human full-length cDNA restored viability and suppressed the G2/M arrest phenotype of dsk2Delta rad23Delta Saccharomyces cerevisiae mutants. Chap2 is a homologue for Xenopus scythe which is an essential component of reaper-induced apoptosis in egg extracts. While the N-terminal UbL domains were not essential for Stch binding, Chap1/Dsk2 contains a Sti1-like repeat sequence that is required for binding to Stch and is also conserved in the Hsp70 binding proteins, Hip and p60/Sti1/Hop. These findings extend the association between Hsp70 members and genes encoding UbL sequences and suggest a broader role for the Hsp70-like ATPase family in regulating cell cycle and cell death events.
Kaye
F J
FJ
Medicine Branch, Division of Clinical Sciences, National Cancer Institute and National Naval Medical Center, Bethesda, MD, USA. fkaye@helix.nih.gov
Modi
S
S
Ivanovska
I
I
Koonin
E V
EV
Thress
K
K
Kubo
A
A
Kornbluth
S
S
Rose
M D
MD
D048868:Adaptor Proteins, Signal Transducing; D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000071183:Autophagy-Related Proteins; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D000071183:Autophagy-Related Proteins; D018797:Cell Cycle Proteins; C100415:DSK2 protein, S cerevisiae; D005656:Fungal Proteins; D018840:HSP70 Heat-Shock Proteins; C086345:HSPA13 protein, human; D029701:Saccharomyces cerevisiae Proteins; C405662:UBQLN2 protein, human; D014452:Ubiquitins; D000251:Adenosine Triphosphatases