The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin.
We have designed a gene cassette for expression of the bleomycin-resistance protein from Streptoalloteichus hindustanus (ShBle) in the extremely halophilic archaeon Haloferax volcanii, and shown that transformed haloarchaea are resistant to bleomycin. Recombinant ShBle was purified by a one-step affinity-chromatography procedure as a correctly folded, dimeric protein. ShBle thus provides a useful haloarchaeal selectable marker and represents the first non-halophilic and soluble heterologous protein to be expressed in the Haloarchaea.
Nuttall
S D
SD
CSIRO Health Sciences and Nutrition, and CRC for Diagnostic Technologies, 343 Royal Parade, Parkville, Victoria 3052, Australia. Stewart.Nuttall@hsn.csiro.au
Deutschel
S E
SE
Irving
R A
RA
Serrano-Gomicia
J A
JA
Dyall-Smith
M L
ML
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001105:Archaea / Q000187:drug effects / Q000235:genetics*; D019843:Archaeal Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001761:Bleomycin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D019766:Genes, Archaeal; D019621:Haloferax / Q000187:drug effects / Q000235:genetics
D000903:Antibiotics, Antineoplastic; D019843:Archaeal Proteins; D001426:Bacterial Proteins; C099353:phleomycin resistance protein, Streptoalloteichus hindustanus; D001761:Bleomycin