Baculovirus expression of the major surface antigen of Toxoplasma gondii and the immune response of mice injected with the recombinant P30.
The major surface antigen (P30) of the Toxoplasma gondii was expressed by an insect cell culture system infected with recombinant baculovirus. About 750 microg of purified (95% purity) P30 was obtained from a culture of 10(8) insect Sf21 cells. The recombinant P30 was used to immunize mice to induce immune response. Mice injected with the recombinant protein produced specific humoral and cellular immune responses. Immunization with P30 also prolonged the period of survival of mice infected by Toxoplasma. The average survival time of control group is 13.25+/-1.16 days, but are 16.13+/-2.1 days, 19.50+/-3.21 days, 20.38+/-3.38 days in different immunized groups, respectively.
Chen
X G
XG
Department of Parasitology, The First Military Medical University, Guang Zhou, China. XGChen@fjnet.guangzhou.gd.cn
Fung
M C
MC
Ma
X
X
Peng
H J
HJ
Shen
S M
SM
Liu
G Z
GZ
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000917:Antibody Formation / Q000276:immunology; D000953:Antigens, Protozoan; D016367:Baculoviridae / Q000235:genetics* / Q000276:immunology*; D015153:Blotting, Western; D003001:Cloning, Molecular / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D015967:Gene Expression Regulation, Viral / Q000235:genetics* / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*; D014162:Transfection
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C047168:SAG1 antigen, Toxoplasma