[A simple and rapid method of preparing cell or tissue embedded in situ for transmission electron microscopy].
A simple and rapid method of preparing cell or tissue embedded in situ for transmission electron microscopy (TEM) is recommended. It is very simple; all procedures, including fixation, dehydration, infiltration and embedding, are performed on the same carrier (culture vase or microscope slides). It is also very rapid; the whole process takes only five hours. The entire cell or tissue embedded in situ is easily separated from the carrier. The ultrastructures of the cell or tissue embedded in situ are well preserved with minimal damage. This method can be used in preparing cultured monolayer cell for TEM study, paraffin section for electron microscopic diagnosis, and frozen section for TEM immunohistochemistry or enzymehistochemistry. The main points of the procedures of preparation have been discussed.
Yang
G
G
Electron Microscopy Laboratory, School of Basic Medical Sciences, Chengdu.
Tang
X
X
D006801:Humans; D008854:Microscopy, Electron; D010857:Pigment Epithelium of Eye / Q000648:ultrastructure; D016610:Tissue Embedding / Q000379:methods*