Expression of CD80 promoter in transgenic mice.
CD80 is a very potent co-stimulatory factor which is required for complete T-cell activation. Here, we use transgenic mice as a tool to map the promoter of the CD80 gene. We engineered three different CD80 promoter driven luciferase transgenes: -3084, -1073 and -215. With these transgenes, we have generated three groups of transgenic mice. Our results showed that the -3084 CD80 promoter/luciferase transgene was sufficient to confer tissue-specific expression of the CD80 gene. When the promoter sequence was deleted to -1073, the normal tissue-specific expression was lost. A brain-specific element was mapped between -1073 nt and -215 nt. This element caused up to ninefold higher expression of the CD80 promoter/luciferase in brain tissue of -1073 CD80 promoter/luciferase transgenic animals as compared to -3084 CD80 promoter/luciferase transgenic animals. In contrast to results with a cell culture system, little luciferase activity was detected in -215 CD80 promoter/luciferase transgenic animals.
Zhang
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H
Department of Growth Factor Research, Pharmaceutical Products Division, Abbott Laboratories D-4MG, J-35, 200 Abbott Park Road, Abbott Park, IL 60064-6181, USA. haiyingzhang@abbott.com
Haasch
D
D
Patterson
B
B
Dickinson
B
B
Okasinski
G F
GF
D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D015870:Gene Expression; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019076:Transgenes
D018122:B7-1 Antigen; D012313:RNA; D008156:Luciferases