[Protective effects of exogenous superoxide dismutase on rabbit retinal injury by acute intraocular hypertension].
OBJECTIVE
To study active oxygen and free radical injury in rabbit retina during elevated intraocular pressure and the protective effect of exogenous superoxide dismutase (SOD) on the retinal damage by the hypertension.


METHODS
Lipid peroxidative product, malondialdehyde (MDA), activity of SOD and glutathione peroxidase (GSH-Px), reduced GSH in the retinal tissue were measured during 24 h after the release of an ocular hypertension, 6.67 kPa (1 kPa = 7.5 mmHg) maintaining for 1.5 h, and the effects of retrobulbarly injected Cu-Zn-SOD on the level of MDA and the activity of SOD in the retinal tissue after the release of ocular hypertension for 12 h were observed.


RESULTS
MDA increased gradually during 0-12 h after the release of ocular hypertension and maintained at a relatively high level in 12-24 h. The activity of SOD and GSH-Px was lower than normal level immediately after the release, and then increased to a certain different extent. But the activity of SOD began to decrease gradually 4 h after the release. GSH had no significant changes during 24 h after the release. Retrobulbar injection of Cu-Zn-SOD reduced the production of MDA in the retinal tissue and enhanced SOD activity.


CONCLUSIONS
Active oxygen and free radicals participate the rabbit retinal injury by elevated intraocular pressure. A high dose of Cu-Zn-SOD retrobulbar injection plays a beneficial role in enhancing the antioxidative ability of the retina.
Bai
Y
Y
Department of Ophthalmology, China Medical University, Shenyang.
Guan
J
J
Pi
J
J
D000818:Animals; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009798:Ocular Hypertension / Q000378:metabolism*; D011817:Rabbits; D012160:Retina / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism / Q000494:pharmacology*
D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase