Positive reactions to common allergens in 42 atopic dogs in Japan.
Clinically important allergens for the diagnosis and treatment of atopic dermatitis vary geographically. In order to identify the most prevalent allergens in atopic dogs in Japan, 42 dogs with a clinical diagnosis of atopy were tested using both in vivo (intradermal skin test (IDST)) and in vitro (antigen-specific IgE assay) allergy tests. Allergens used for IDST included 26 allergen extracts from eight allergen groups: trees, weeds, grasses, house dust mites (HDM), molds, foods, epithelia, and arthropods. Immunodot assay was used to measure antigen-specific IgE against 24 allergens from these eight groups and against fish such as cod and sole. In the 42 dogs, the most common positive allergen reaction was to HDM on both IDST (29/42 dogs or 69%) and in vitro testing (23/42 or 54.8%). The second most frequent positive allergen reaction was to Japanese cedar pollen (21/42 or 50.0% for IDST and 7/42 or 16.7% for in vitro testing). In both tests, less than 20% of dogs had positive reactions to molds or foods. Positive reactions to cat epithelia were frequently found on IDST, but rarely found on in vitro testing. Agreement between the two tests was found in 26 instances: HDM (21 dogs), Japanese cedar pollen (five dogs) and wheat (one dog). In this study, the two most common allergens involved in atopic dermatitis in dogs in Japan were HDM and Japanese cedar pollen.
Masuda
K
K
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. amasuda@hongo.ecc.u-tokyo.ac.jp
Sakaguchi
M
M
Fujiwara
S
S
Kurata
K
K
Yamashita
K
K
Odagiri
T
T
Nakao
Y
Y
Matsuki
N
N
Ono
K
K
Watari
T
T
Hasegawa
A
A
Tsujimoto
H
H
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D001181:Arthropods; D015153:Blotting, Western / Q000662:veterinary; D003876:Dermatitis, Atopic / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000276:immunology*; D004285:Dogs; D004391:Dust; D005260:Female; D005658:Fungi; D007428:Intradermal Tests / Q000662:veterinary; D007564:Japan / Q000453:epidemiology; D008297:Male; D008925:Mites; D006109:Poaceae; D011058:Pollen / Q000276:immunology*; D015995:Prevalence; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D014197:Trees
D000485:Allergens; D000911:Antibodies, Monoclonal; D004391:Dust; D011933:Reagent Kits, Diagnostic