[Expression of metastasis-suppressor gene nm23H1 product in nasopharyngeal carcinoma].
Using the specific monoclonal antibody of nm23H1 gene product and the immunohistochemical technique, we studied the expression of DNPK/nm23H1 and its correlation with lymph-node metastasis in nasopharyngeal carchnoma (NPC). The results revealed that, in a total of 31 cases of NPC, 13 showed positive expression with a positive rate of 41.9%. Among 21 cases of NPC with no lymph-node metastasis, 11 (52.3%) showed positive expression while only 20% (2/10) of those with lymph-node metastasis were positive, and there was significant difference between them (P < 0.01). Meantime the expression was very low or not detectable in the cancerous cells of the match lymph-node metastasis and all 10 cases showed negative expression. Statistical significant difference was found between the group of NPC with no metastasis and the group of lymph-node metastasis (P < 0.01). The above results indicated that high expression of DNPK/nm23H1 existed in the group of NPC with no metastasis while low expression existed in those with metastasis and lymph-node metastasis. It suggests that the expression of nm23H1 is inversely associated with lymph-node metastasis. The DNPK/nm23H1 may play a role in polymerization of microtubulin protein and participate in the process of cancer metastasis.
Huang
G
G
Institute of Cancer Research, Chengdu.
Zeng
X
X
Lu
Y
Y
Ning
Q
Q
Yang
C
C
Jiang
Y
Y
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis*; D054778:NM23 Nucleoside Diphosphate Kinases; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009701:Nucleoside-Diphosphate Kinase; D014157:Transcription Factors / Q000096:biosynthesis*
D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins