title: Cariostatic effect of a two-part fluoride dentifrice in rats.
abstract: This study compared the cariostatic effect of a two-part, fluoride-calcium-phosphate-containing prototype dentifrice (containing 1100 ppm F) to a conventional, clinically proven effective "gold standard" (1100 ppm F, USP Standard) and an Enamelon placebo dentifrice (0 ppm F) using a rat model. Three groups of 18 Crl:CDR(SD)BR rats were inoculated with S. sobrinus 27352 and fed MIT-200 for 35 days in a programmed feeder. Double-deionized water (DDW) was given ad libitum. The rats were treated twice daily with one two-part dentifrice. Just prior to treatment, each of the two parts of dentifrice were mixed 1:1 and applied undiluted. The rats were euthanized and their mandibular molars scored for caries using the Keyes quantification method. Shrestha SNP caries scores were then calculated. Non-parametric comparisons were done among treatments at the 0.05 level of significance using analyses based on ranks. The mean Shrestha SNP Caries Scores +/- standard deviations for the prototype dentifrice, USP standard and Enamelon placebo were 84.9 +/- 62.8, 101.3 +/- 66.3, and 181.2 +/- 100.1, respectively. Scores for the 1100 ppm F prototype treatment were significantly lower than the USP Standard and the Enamelon placebo. These results indicate that the new prototype dentifrice, containing fluoride-calcium-phosphate, increased the anti-caries efficacy in a rat model when compared with a fluoride-containing USP Standard dentifrice.
authors.affiliation: University of Rochester Eastman Department of Dentistry, NY, USA. sheila_shrestha@urmc.rochester.edu
authors.forename: S A
authors.initials: SA
authors.lastname: Mundorff-Shrestha
authors.forename: H M
authors.initials: HM
authors.lastname: Proskin
authors.forename: A E
authors.initials: AE
authors.lastname: Winston
authors.forename: C W
authors.initials: CW
authors.lastname: Triol
authors.forename: G
authors.initials: G
authors.lastname: Cornell
authors.forename: T
authors.initials: T
authors.lastname: Sharpe
mesh_terms: D000287:Administration, Topical; D000704:Analysis of Variance; D000821:Animal Feed; D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D002327:Cariostatic Agents / Q000737:chemistry / Q000627:therapeutic use*; D003731:Dental Caries / Q000517:prevention & control*; D003802:Dentifrices / Q000737:chemistry / Q000627:therapeutic use*; D005459:Fluorides / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric
chemical_list: D002130:Calcium Phosphates; D002327:Cariostatic Agents; D003802:Dentifrices; C118793:Enamelon; D005459:Fluorides