title: Adenovirus-mediated ribozyme targeting of HER-2/neu inhibits in vivo growth of breast cancer cells.
abstract: HER-2/neu is overexpressed in 25-30% of human breast cancers. We prepared an anti-HER-2/neu hammerhead ribozyme expressed by a recombinant adenovirus (rAdHER-Rz). Human breast cancer cell lines were transduced with high efficiency, resulting in decreased HER-2/neu expression. In vivo injections of rAdHER-Rz into BT-474 tumors established in nude mice inhibited tumor growth to 20% of mock-treated controls. Similar in vivo effects were shown in MCF-7 cells, which do not overexpress HER-2/neu. The growth inhibitory effects of rAdHER-Rz were greater than those of an antisense-expressing vector. These results suggest the utility of anti-HER-2/neu ribozymes as a rational strategy for gene therapy of breast cancer. Gene Therapy (2000) 7, 241-248.
authors.affiliation: Cancer Research Department, Berlex Biosciences, Richmond, CA, USA.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: B
authors.initials: B
authors.lastname: Anderegg
authors.forename: T
authors.initials: T
authors.lastname: Ohkawa
authors.forename: A
authors.initials: A
authors.lastname: Irie
authors.forename: O
authors.initials: O
authors.lastname: Engebraaten
authors.forename: M
authors.initials: M
authors.lastname: Halks-Miller
authors.forename: P S
authors.initials: PS
authors.lastname: Holm
authors.forename: D T
authors.initials: DT
authors.lastname: Curiel
authors.forename: M
authors.initials: M
authors.lastname: Kashani-Sabet
authors.forename: K J
authors.initials: KJ
authors.lastname: Scanlon
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy*; D002455:Cell Division; D018390:Gene Targeting / Q000379:methods*; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D016337:RNA, Catalytic / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D016337:RNA, Catalytic; C121505:Rz967