Effects of allitridi on intracellular Ca2+ concentration in isolated rat brain cells.
OBJECTIVE
To study actions of allitridi extracted from garlic on intracellular calcium in isolated rat brain cells.


METHODS
Brain cells were isolated from newborn rat brain with Fura 2-AM measurements of intracellular Ca2+ concentration ([Ca2+]i).


RESULTS
Allitridi 1-100 mumol.L-1 concentration-dependently blocked increases of [Ca2+]i caused by potassium chloride and sodium glutamate (Glu) with IC50 of 59.7 and 69.9 mumol.L-1 respectively. Allitridi 100 mumol.L-1 blocked norepinephrine (Nor)-induced [Ca2+]i elevation.


CONCLUSIONS
Allitridi is an effective agent for blocking the [Ca2+]i increase caused by potassium chloride, Nor and Glu.
Ma
X H
XH
Department of Pharmacology, Nanjing Medical University, China.
Pan
X X
XX
Liu
T P
TP
D000498:Allyl Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002469:Cell Separation; D005737:Garlic / Q000737:chemistry*; D009638:Norepinephrine / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal; D011189:Potassium Chloride / Q000037:antagonists & inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D012970:Sodium Glutamate / Q000037:antagonists & inhibitors; D013440:Sulfides / Q000302:isolation & purification / Q000494:pharmacology*
D000498:Allyl Compounds; D013440:Sulfides; C042577:diallyl trisulfide; D011189:Potassium Chloride; D002118:Calcium; D012970:Sodium Glutamate; D009638:Norepinephrine