p97 ATPase, an ATPase involved in membrane fusion, interacts with DNA unwinding factor (DUF) that functions in DNA replication.
DNA unwinding factor (DUF) unwinds duplex DNA and is supposed to function in DNA replication in Xenopus egg extracts. Here we report the isolation and analysis of a DUF-interacting factor. By immunoprecipitation, we found that p97 ATPase (p97) interacts with DUF in Xenopus egg extracts. This interaction was confirmed by the in vitro binding of purified p97 with DUF. When sperm chromatin was added to Xenopus egg extracts to construct nuclei active in DNA replication, p97 was incorporated into the nuclei. These data suggest that the complex of DUF and p97 may function in DNA replication.
Yamada
T
T
Cellular and Molecular Biology Laboratory, Saitama, Japan. tyamada@postman.riken.go.jp
Okuhara
K
K
Iwamatsu
A
A
Seo
H
H
Ohta
K
K
Shibata
T
T
Murofushi
H
H
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D004265:DNA Helicases / Q000378:metabolism*; D004261:DNA Replication; D008561:Membrane Fusion; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014981:Xenopus
D009687:Nuclear Proteins; D000251:Adenosine Triphosphatases; C404942:p97 ATPase; D004265:DNA Helicases