title: A polymorphism in the mouse crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay.
abstract: The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie and Hygiene, Universität Freiburg, Hermann-Herder-Strasse 11, Freiburg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Hallensleben
authors.forename: L
authors.initials: L
authors.lastname: Biro
authors.forename: C
authors.initials: C
authors.lastname: Sauder
authors.forename: J
authors.initials: J
authors.lastname: Hausmann
authors.forename: V C
authors.initials: VC
authors.lastname: Asensio
authors.forename: I L
authors.initials: IL
authors.lastname: Campbell
authors.forename: P
authors.initials: P
authors.lastname: Staeheli
mesh_terms: D000818:Animals; D001483:Base Sequence; D054357:Chemokine CXCL10; D019743:Chemokines, CXC / Q000235:genetics*; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D008969:Molecular Sequence Data; D015846:Monokines / Q000235:genetics*; D011110:Polymorphism, Genetic; D011933:Reagent Kits, Diagnostic; D012260:Ribonucleases
chemical_list: D054357:Chemokine CXCL10; D019743:Chemokines, CXC; C485433:Cxcl10 protein, mouse; D018076:DNA, Complementary; D015846:Monokines; D011933:Reagent Kits, Diagnostic; D012260:Ribonucleases