title: Copper-induced conformational changes in the N-terminal domain of the Wilson disease copper-transporting ATPase.
abstract: The Wilson disease copper-transporting ATPase plays a critical role in the intracellular trafficking of copper. Mutations in this protein lead to the accumulation of a toxic level of copper in the liver, kidney, and brain followed by extensive tissue damage and death. The ATPase has a novel amino-terminal domain ( approximately 70 kDa) which contains six repeats of the copper binding motif GMTCXXC. We have expressed and characterized this domain with respect to the copper binding sites and the conformational consequences of copper binding. A detailed analysis of this domain by X-ray absorption spectroscopy (XAS) has revealed that each binding site ligates copper in the +1 oxidation state using two cysteine side chains with distorted linear geometry. Analysis of copper-induced conformational changes in the amino-terminal domain indicates that both secondary and tertiary structure changes take place upon copper binding. These copper-induced conformational changes could play an important role in the function and regulation of the ATPase in vivo. In addition to providing important insights on copper binding to the protein, these results suggest a possible mechanism of copper trafficking by the Wilson disease ATPase.
authors.affiliation: Department of Structural Biology and Biochemistry, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
authors.forename: M
authors.initials: M
authors.lastname: DiDonato
authors.forename: H F
authors.initials: HF
authors.lastname: Hsu
authors.forename: S
authors.initials: S
authors.lastname: Narindrasorasak
authors.forename: L
authors.initials: L
authors.lastname: Que
authors.forename: B
authors.initials: B
authors.lastname: Sarkar
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D027682:Cation Transport Proteins; D002942:Circular Dichroism; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D000073840:Copper-Transporting ATPases; D006801:Humans; D008954:Models, Biological; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013057:Spectrum Analysis; D014965:X-Rays
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D010446:Peptide Fragments; D003300:Copper; D000251:Adenosine Triphosphatases; D000073840:Copper-Transporting ATPases