IL-10 contributes to the inhibition of contact hypersensitivity in mice treated with photodynamic therapy.
We have explored the effect of photodynamic therapy (PDT) with verteporfin on the induction and expression of contact hypersensitivity (CHS) to 2,4-dinitrofluorobenzene (DNFB) in normal mice and IL-10-deficient mice. Our results indicate that DNFB sensitized mice given PDT with verteporfin and whole body red light irradiation exhibited a significant reduction in CHS compared with control animals. Administration of rIL-12 reversed the effect(s) of PDT as did treatment of mice with anti-IL-10-neutralizing Ab. Knockout mice deficient in IL-10 were found to be resistant to the inhibitory effects of PDT. In vitro proliferative responses using spleen cells from DNFB-sensitized and PDT-treated mice showed a significantly lower response to DNBS as compared with cells from DNFB-sensitized mice or DNFB and PDT-treated IL-10-deficient mice. Finally, naive mice exposed to PDT exhibited an increase in skin IL-10 levels, which peaked between 72 and 120 h post-PDT. Together these data support the role of IL-10 as a key modulator in the inhibition of the CHS response by whole body PDT.
Simkin
G O
GO
QLT PhotoTherapeutics Inc., Vancouver, BC, Canada. gsimkin@qlt-pdt.com
Tao
J S
JS
Levy
J G
JG
Hunt
D W
DW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D003877:Dermatitis, Contact / Q000188:drug therapy / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D004139:Dinitrofluorobenzene / Q000276:immunology; D004431:Ear, External / Q000276:immunology; D005260:Female; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010778:Photochemotherapy / Q000379:methods; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D016753:Interleukin-10; D004139:Dinitrofluorobenzene