title: Cloning and expression of cytochrome P450 genes belonging to the CYP4 family and to a novel family, CYP48, in two hymenopteran insects, Trichogramma cacoeciae and Apis mellifera.
abstract: Cytochrome P450 partial sequences were isolated by PCR using genomic DNA from two hymenopteran insects of agronomical importance, Trichogramma cacoeciae, a parasitoid wasp, and Apis mellifera, the honeybee. Four new P450 genes were identified: one honeybee gene belongs to the CYP4 family and was named CYP4G11; the three other genes were from Trichogramma and belong to the CYP4 family (CYP4G12) and to a novel family, the CYP48 one (CYP48A1 and CYP48A2). The four genes contain a short intron (72-95 bp) at the same position as already described for other insect species. The two genes CYP48A1 and CYP48A2 have a supernumary intron (57-71 bp) upstream the first one. Only the two CYP4 genes were constitutively transcribed, at a high level for CYP4G12 and at a low level for CYP4G11. No expression was observed for CYP48A1 and CYP48A2.
authors.affiliation: INRA-IFR 38 "Réponse des organismes aux stress physico-chimiques et biologiques,", Unité Santé Végétale et Environnement, 123 boulevard Francis Meilland, Antibes Cedex, 06606, France. tares@antibes.inra.fr
authors.forename: S
authors.initials: S
authors.lastname: Tarès
authors.forename: J B
authors.initials: JB
authors.lastname: Bergé
authors.forename: M
authors.initials: M
authors.lastname: Amichot
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D017344:Genes, Insect; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014863:Wasps / Q000201:enzymology* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D003577:Cytochrome P-450 Enzyme System