title: The C1orf9 gene encodes a putative transmembrane member of a novel protein family.
abstract: Here we report the characterization of a human mRNA encoding a novel protein denoted C1orf9 (chromosome 1 open reading frame 9). The cDNA sequence, derived from a testis cDNA library, contains 5700 bp which encodes an open reading frame of 1254 amino acids. The deduced protein contains a putative N-terminal signal peptide and one putative transmembrane region, indicating membrane localization. No significant homology was found with known characterized proteins. However, a 150 amino acid region has significant homology to deduced protein sequences from other organisms, including Caenorhabditis elegans (43% identity), Saccharomyces cerevisiae (47% identity), Schizosaccharomyces pombe (48% identity), and two proteins from Arabidopsis thaliana (42% and 40% identity), suggesting a novel family of conserved domains. The C1orf9 gene was assigned to chromosome 1q24. The gene spans approximately 78.7 kb and is organized into at least 24 exons. Expression analysis revealed a single C1orf9 mRNA species of approximately 6.0 kb with a predominant expression in pancreas and testis, and only low levels of expression in other tissues examined.
authors.affiliation: Department of Immunology, Norwegian Radium Hospital, Montebello, Oslo, N-0310, Norway.
authors.forename: O
authors.initials: O
authors.lastname: Røsok
authors.forename: F
authors.initials: F
authors.lastname: Pedeutour
authors.forename: J
authors.initials: J
authors.lastname: Odeberg
authors.forename: J
authors.initials: J
authors.lastname: Lundeberg
authors.forename: H C
authors.initials: HC
authors.lastname: Aasheim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005091:Exons; D015723:Gene Library; D006801:Humans; D007438:Introns; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D021382:Protein Sorting Signals / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; C404748:SUCO protein, human