title: Detection of integrins in cataract lens epithelial cells.
abstract: OBJECTIVE
  To detect the expression of integrin subunits in lens epithelial cells (LECs) of human cataracts.
  
  
  METHODS
  Research Laboratory, International Intraocular Implant Training Centre, Tianjin Medical University, China.
  
  
  METHODS
  The circular sections of the anterior capsules with attached LECs were obtained during cataract surgery from 100 patients. The LECs were stained with an avidin-biotin-complex immunohistochemical technique using 5 monoclonal antibodies specific for alpha subunits 2, 3, 5 and beta subunits 1, 2.
  
  
  RESULTS
  All integrin subunits studied were found to varying degrees in specimens. The positive percentages were 70%, 65%, 75%, 70%, and 80%, respectively.
  
  
  CONCLUSIONS
  Integrin subunits were present in LECs of human cataracts. These molecules may serve in the adhesion of LECs to the lens capsule and play a role in cell-posterior capsule interaction after cataract surgery.
authors.affiliation: International Intraocular Implant Training Centre, Tianjin Medical University, China.
authors.forename: X H
authors.initials: XH
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Ji
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Tang
authors.forename: H M
authors.initials: HM
authors.lastname: Sun
authors.forename: J Q
authors.initials: JQ
authors.lastname: Yuan
mesh_terms: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D002386:Cataract / Q000378:metabolism*; D002387:Cataract Extraction; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D016023:Integrins / Q000378:metabolism*; D007903:Lens Capsule, Crystalline / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D016023:Integrins