[The expressions of p16, CDK4 and PCNA proteins in trophoblastic tumors].
OBJECTIVE
To evaluate the relationship between the regulatory factors in G1 phase of cell cycle and the cacrinogenesis of the trophoblastic cells.


METHODS
The expressions of p16, cyclin-dependent kinase 4 (CDK4), proliferation cell nuclear antigen (PCNA) proteins in 18 cases of trophoblastic tumors, 30 cases of hydatidiform mole and 30 cases of normal villi were studied by immunohistochemical methods.


RESULTS
The expressions of p16 protein between malignant trophoblastic tumors and normal villi were significantly different (P < 0.05). PCNA positive rate in p16 positive samples were markedly lower than that in negative samples (P < 0.05). The expressions of CDK4 among normal villi, hydatiform mole and trophoblastic tumors were not significantly different. Patients with positive p16 expression had a higher 3 year survival rate.


CONCLUSIONS
p16 protein may inhibit trophoblastic cells proliferation, and p16 gene mutation may be an important factor in carcinogenesis of trophoblastic cells and proliferation out of control. P16 protein expression examination is helpful in prediction of patients prognosis.
Fu
C
C
Department of Gynecology and Obstetry, Second Affiliated Hospital, Hunan Medical University, Changsha.
Zhang
Q
Q
Lu
F
F
Zhang
Z
Z
Hu
Y
Y
Lin
L
L
D002822:Choriocarcinoma / Q000378:metabolism*; D002824:Chorionic Villi / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis*; D018844:Cyclin-Dependent Kinases / Q000096:biosynthesis*; D005260:Female; D005500:Follow-Up Studies; D016193:G1 Phase; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D011518:Proto-Oncogene Proteins; D014594:Uterine Neoplasms / Q000378:metabolism*
D019941:Cyclin-Dependent Kinase Inhibitor p16; D018809:Proliferating Cell Nuclear Antigen; D011518:Proto-Oncogene Proteins; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases