Preparation and culture of a serum-free human thyroid follicle system and its application for measuring thyroid hormone secretion, iodide uptake and organification, cyclic adenosine monophosphate formation, gene expression, and cell growth.
We describe a system of human thyroid follicles cultured in collagen suspended in serum-free medium. The method allows measurement of thyroid hormone secretion, iodide uptake and organification, cyclic adenosine monophosphate (cAMP) formation, gene expression, as well as cell growth. The system is superior to follicles freely suspended in cultured medium and also, as demonstrated by parallel experiments, to monolayer culture systems. A detailed description of the optimal conditions of the method is provided that, over a period of 8 years, has proven to be a powerful tool for measuring thyroid cell function, gene expression and cell proliferation.
Kraiem
Z
Z
Endocrine Research Unit, Carmel Medical Center, Haifa and Technion Faculty of Medicine, Israel. zkraiem@tx.technion.ac.il
Sadeh
O
O
Heinrich
R
R
D000818:Animals; D002455:Cell Division; D016895:Culture Media, Serum-Free; D046508:Culture Techniques; D000242:Cyclic AMP / Q000096:biosynthesis*; D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D006652:Histological Techniques; D006801:Humans; D007454:Iodides / Q000493:pharmacokinetics*; D013936:Thymidine / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D014284:Triiodothyronine / Q000235:genetics* / Q000378:metabolism*
D016895:Culture Media, Serum-Free; D007454:Iodides; D014284:Triiodothyronine; D004247:DNA; D000242:Cyclic AMP; D013936:Thymidine