[The experimental studies of immune response of antigen-extracted bovine cancellous bone grafting].
Xenogeneic bone grafting is an alternative to autogeneic bone grafting, but the intense immune rejection makes its clinical practice limited. We performed lymphocytes proliferation assay, enzyme-linked immunosorbent assay, and histological observation to evaluate the levels of cellular and humoral immunity, and the tissue reaction to the grafting of BALB/c mice receiving fresh bovine cancellous bone (FCB), antigen-extracted bovine massive cancellous bone (MCB), antigen-extracted bovine granular cancellous bone (GCB). Lymphocyte proliferation was increased in an early phase of the grafting and persisted for a long period in FCB group. Meanwhile higher levels of specific antibody were detected. In MCB and GCB groups, lymphocyte proliferation and specific antibody production were not ascended and no significant difference was observed between MCB and GCB groups in these immunological responses. There were no appreciable histologic signs of immune or foreign body reaction both in MCB and GCB groups. The results suggested that MCB, GCB were characterized less antigenicity and can be used as osteoconductive material or a carrier of bone growth factors.
Luo
Z
Z
Department Orthopaedics Surgry of Xijing Hospital, 4th Military Medical University, Xi'an.
Hu
Y
Y
Wang
Q
Q
D000818:Animals; D000941:Antigens; D016025:Bone Transplantation; D001842:Bone and Bones / Q000276:immunology*; D002417:Cattle; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014183:Transplantation, Heterologous / Q000379:methods
D000941:Antigens