Crystallization and preliminary crystallographic studies of ribosome recycling factor from Escherichia coli.
Ribosome recycling factor (RRF) catalyzes the disassembly of a termination complex during the final stage of protein synthesis. RRF from Escherichia coli has been crystallized with PEG 400 as precipitant at 287 K. The crystal belongs to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell parameters a = b = 48.08, c = 141.67 A. Native data were collected from a frozen crystal to a resolution of 3.0 A on a Cu Kalpha rotating-anode X-ray source.
Yun
J
J
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151--742, South Korea.
Kim
W
W
Ha
S C
SC
Eom
S H
SH
Suh
S W
SW
Kim
K K
KK
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000378:metabolism
D001426:Bacterial Proteins; D011506:Proteins; D012269:Ribosomal Proteins; C087267:ribosome releasing factor