title: L-type calcium channels in the hippocampus and cerebellum of Alzheimer's disease brain tissue.
abstract: There is growing evidence that the selective neuronal cell death observed in Alzheimer's Disease (AD) is the result of dysregulation of intracellular calcium (Ca2+) homeostasis. In the present study, L-type voltage sensitive calcium channels (L-VSCCs) were examined in the cerebellum and hippocampus of AD (n = 6; postmortem interval less than 5 h) and age-matched control (n = 6) tissue by homogenate binding techniques and quantitative in vitro receptor autoradiography using [3H]isradipine (PN200-110). Saturation analyses of the cerebellum revealed unaltered [3H]isradipine binding parameters (Kd and Bmax) between AD and control subjects. Analysis of AD and control hippocampus demonstrated significant differences as [3H]isradipine binding increased (62%) in AD, whereas hippocampal cell density decreased (29%) in AD, relative to control subjects. Moreover, AD differentially affected L-VSCC in area CA1 and dentate gyrus. The dentate gyrus had greatly increased binding (77%) with little cell loss (16%) in AD brains, whereas area CA1 had increased binding (40%) with significant cell loss (42%) in AD brains, relative to controls. The results of the present study suggest that hippocampal area CA1 may experience greater cell loss in response to increased L-VSCCs in AD relative to other brain regions.
authors.affiliation: University of Kentucky, Department of Anatomy and Neurobiology, Lexington 40536-0298, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Coon
authors.forename: D R
authors.initials: DR
authors.lastname: Wallace
authors.forename: C F
authors.initials: CF
authors.lastname: Mactutus
authors.forename: R M
authors.initials: RM
authors.lastname: Booze
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology*; D001345:Autoradiography; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism* / Q000473:pathology*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007700:Kinetics
chemical_list: D020746:Calcium Channels, L-Type