Comparison of FaRP immunoreactivity in free-living nematodes and in the plant-parasitic nematode Heterodera glycines.
The family of FMRFamide-related peptides (FaRPs) is widely distributed among invertebrates, where the peptides serve as neuromodulators. Published reports indicate that numerous FaRP sequences exist in free-living and animal parasitic nematodes. Using a FMRFamide ELISA, FaRP immunoreactivity was detected in extracts of the soybean cyst nematode, Heterodera glycines, in both sexes and at all developmental stages. HPLC-ELISA results revealed a number of immunoreactive components in H. glycines preparations, and a comparison with extracts of the free-living nematodes Caenorhabditis elegans and Panagrellus redivivus showed significant qualitative differences in FaRP immunoreactivity between the plant parasite and the two free-living nematodes. Total and specific immunoreactivities varied during H. glycines development, with the highest specific activity in juveniles and males, and the highest total activity in mature females. Total female immunoreactivity was located primarily within the mature eggs. A significant portion, however, was associated with the female body, perhaps with egg laying.
Masler
E P
EP
Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705-2350, USA. emasler@asrr.arsusda.gov
Kovaleva
E S
ES
Sardanelli
S
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D019835:FMRFamide / Q000031:analogs & derivatives* / Q000032:analysis*; D005260:Female; D009348:Nematoda / Q000737:chemistry* / Q000254:growth & development; D009479:Neuropeptides / Q000032:analysis*; D013025:Glycine max / Q000469:parasitology*
D009479:Neuropeptides; D019835:FMRFamide