Characterization of a cDNA encoding a rice mitochondrial voltage-dependent anion channel and its gene expression studied upon plant development and osmotic stress.
The voltage-dependent anion channel (VDAC) of mitochondria forms a large pore in the outer envelope membrane. Here, the full Oryza sativa OSVDAC1 cDNA was sequenced and is shown to belong to a small multigene family in the rice genome. This cDNA is 1093 bp long and codes for a protein of 274 amino acids. Expression studies of the osvdac1 gene show a regulation of its level in function of the plantlets maturation and organs. In contrast with several bacterial porins, osmotic stress does not have any effect on the plant osvdac1 gene expression.
Roosens
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N
Laboratorium voor Plantengenetica, Vrije Universiteit Brussel, Paardenstraat 65, B1640, Sint Genesius Rode, Belgium.
Al Bitar
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F
Jacobs
M
M
Homblé
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007425:Intracellular Membranes / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D009994:Osmolar Concentration; D018532:Pisum sativum / Q000235:genetics; D018272:Porins / Q000737:chemistry / Q000235:genetics*; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000235:genetics; D014908:Triticum / Q000235:genetics; D050994:Voltage-Dependent Anion Channels; D003313:Zea mays / Q000235:genetics
D018076:DNA, Complementary; D018272:Porins; D050994:Voltage-Dependent Anion Channels