Ro 31-8220 inhibits release of interleukin-1 and interleukin-6 from mouse peritoneal macrophages induced by fibrin fibrinogen degradation products.
OBJECTIVE
To study the effect of fibrin fibrinogen degradation products (FFDP) on release of interleukin-1 (IL-1) and interleukin-6 (IL-6) from mouse peritoneal macrophages, and the effect of a new selectively potent protein kinase C inhibitor Ro 31-8220 (Ro).


METHODS
IL-1 and IL-6 activities were measured by thymocyte proliferation assay and B9 cell proliferation methyl thiazolyl tetrazolium (MTT) colorimetric method, respectively.


RESULTS
Ro 0.01-1 mumol.L-1 obviously inhibited FFDP-induced release of IL-1 and IL-6 from mouse peritoneal macrophages.


CONCLUSIONS
Ro exerted inhibitory effects on FFDP-induced release of IL-1 and IL-6 in vitro.
Zhou
B
B
Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai, China. zhoubin@ecmu.org.cn
Zhang
J P
JP
Hu
Z L
ZL
Tan
Y X
YX
Qian
D H
DH
D000818:Animals; D005338:Fibrin Fibrinogen Degradation Products / Q000037:antagonists & inhibitors; D007211:Indoles / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*
D005338:Fibrin Fibrinogen Degradation Products; D007211:Indoles; D007375:Interleukin-1; D015850:Interleukin-6; D011493:Protein Kinase C; C064758:Ro 31-8220