Nerve growth factor treatment alters Ca2+ pump levels in PC12 cells.
Nerve growth factor (NGF) treatment converts rapidly dividing PC12 cells into a neuronal phenotype. To understand the Ca2+ sequestration mechanisms accompanying this differentiation, we examined the endoplasmic reticulum Ca2+ (SERCA) pump levels using two different assays: ATP-dependent azide insensitive oxalate stimulated 45Ca2+ uptake by PC12 cells permeabilized with saponin, and Western blots using a monoclonal antibody which reacts with all the SERCA isoforms. We also examined the reaction to an antibody against the plasma membrane Ca2+ (PMCA) pump. NGF treatment decreased the SERCA pump expression but it increased the PMCA pump level. These results are consistent with a greater role of PMCA pumps in neuronal cells than in most other cells and with an increased role of SERCA pumps during cell proliferation.
Keller
D
D
Department of Medicine, McMaster University, Hamilton, Canada.
Grover
A K
AK
D000818:Animals; D015153:Blotting, Western; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D002132:Calcium Radioisotopes; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis*; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D015398:Signal Transduction / Q000502:physiology
D015220:Calcium Channels; D002132:Calcium Radioisotopes; D020932:Nerve Growth Factor; D000252:Calcium-Transporting ATPases