title: Reduced brain edema after traumatic brain injury in mice deficient in P-selectin and intercellular adhesion molecule-1.
abstract: Platelet (P-) selectin and intercellular adhesion molecule-1 (ICAM-1) mediate accumulation of neutrophils in brain. However, the mechanisms regulating neutrophil accumulation and damage after traumatic brain injury (TBI) are poorly defined. We hypothesized that mice deficient in both P-selectin and ICAM-1 (-/-) would have decreased brain neutrophil accumulation and edema, and improved functional and histopathological outcome after TBI compared with wild-type (+/+). In Protocol I, neutrophils and brain water content were quantified at 24 h after TBI. No difference in brain neutrophil accumulation was observed between groups; however, brain edema was decreased in dual P-selectin and ICAM-1 -/- (P < 0.05 vs. +/+ mice). In Protocol II, after TBI, tests of motor and memory function and histopathology were assessed over 21 days. No difference in motor or memory function or histopathological damage was observed between +/+ and -/- mice. A role for adhesion molecules in the pathogenesis of brain edema independent of leukocyte accumulation in brain is suggested.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh, Pennsylvania, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Whalen
authors.forename: T M
authors.initials: TM
authors.lastname: Carlos
authors.forename: C E
authors.initials: CE
authors.lastname: Dixon
authors.forename: P
authors.initials: P
authors.lastname: Robichaud
authors.forename: R S
authors.initials: RS
authors.lastname: Clark
authors.forename: D W
authors.initials: DW
authors.lastname: Marion
authors.forename: P M
authors.initials: PM
authors.lastname: Kochanek
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D001930:Brain Injuries / Q000150:complications* / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D003288:Contusions / Q000150:complications / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology*; D008297:Male; D008569:Memory Disorders / Q000209:etiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009069:Movement Disorders / Q000209:etiology; D019007:P-Selectin / Q000235:genetics / Q000502:physiology*; D010538:Peritonitis / Q000276:immunology; D014949:Wounds, Nonpenetrating / Q000150:complications / Q000378:metabolism
chemical_list: D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1