Influence of column design on process-scale ion-exchange chromatography.
The performance of two new designs of pump-packed axial flow process chromatography columns have been evaluated for the preparative anion-exchange chromatography of hen egg-white proteins using Whatman Express-Ion Exchanger Q. A 16 1 Side-Pack column and a 24 1 IsoPak column containing Express-Ion Q were used in this study. In each case ca. 20 1 feedstock containing 5-7 g protein/l, was applied per litre packed bed at flow-rates of ca. 150 and 300 cm/h. In each case the ovalbumin binding capacity was ca. 70 g/l packed bed with ca. 100% (w/w) recovery of applied protein. A clean-in-place procedure involving storage in 0.5 M NaOH was effective in maintaining chromatographic performance in all cases. These data were consistent with our previous work using the more traditionally configured slurry-packed axial flow columns. Each of these column designs were easy to use facilitating rapid packing with this adsorbent and in the case of IsoPak rapid pump unpacking. The introduction of these column designs significantly improves the task of column packing, hitherto a labour intensive, physically demanding and potentially unreproducible process.
Levison
P R
PR
Whatman International Ltd., Maidstone, UK. peterl@whatman.co.uk
Hopkins
A K
AK
Hathi
P
P
D000818:Animals; D000838:Anions; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D004527:Egg Proteins / Q000302:isolation & purification*; D004529:Egg White / Q000032:analysis; D005260:Female; D010047:Ovalbumin / Q000302:isolation & purification
D000838:Anions; D004527:Egg Proteins; D010047:Ovalbumin