[Detecting expression of the multidrug resistance gene product (P170) in human tumor tissues and cells by flow cytometry].
We have detected the human multidrug resistance gene (MDR1) product P170 in 29 solid tumor samples and K562 cell line through indirectimmunoflourence staining by flow cytometry using mouse monoclonal antibody (McAb). The results showed that the expression of P170 was detected in 18 samples, the positive ratio being 62.1%; the expression was not detected in 11 samples, the negative ratio being 37.9%; and 99.9% of K562 cells expressed P170. In 16 of the 18 positive samples, the percent ratio of tumor cells for expression of P170 was less than 30%; in the other 2, more than 30%. This indicated that the positive ratio of P170 of most tumor samples was high, but their percent ratio of P170 was low. Thus it provided a parameter for reference in evaluating the efficacy of clinical antitumor treatments.
Kan
B
B
Center for Biotherapy of Cancer, First Affiliated Hospital, Chengdu.
Luo
F
F
Lei
S
S
Mao
Y
Y
Zou
L
L
Zhao
X
X
Wei
Y
Y
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D019450:Genes, MDR; D006801:Humans; D020014:K562 Cells / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D012004:Rectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1