Crystallization and preliminary X-ray analysis of birch-pollen allergen Bet v 1 in complex with a murine monoclonal IgG Fab' fragment.
The human type I allergic response is characterized by the presence of allergen-specific serum immunoglobulin E (IgE). Allergen-mediated cross-linking of receptor-bound IgE on the surface of mast cells and circulating basophils triggers the release of mediators, resulting in the development of the clinical symptoms of allergy. In order to study the structural basis of allergen-antibody interaction, a complex between the major birch-pollen allergen Bet v 1 and a Fab' fragment isolated from the murine monoclonal Bet v 1 antibody BV16 has been crystallized. Complex crystals belong to space group P1, with unit-cell parameters a = 91.65, b = 99.14, c = 108.90 A, alpha = 105.7, beta = 98.32, gamma = 97.62 degrees, and diffract to 2.9 A resolution when analyzed at 100 K using synchrotron-generated X--rays.
Spangfort
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MD
Biochemical Allergy Research, ALK-Abelló, Boge Allé 6-8, DK-2970 Hørsholm, Denmark. michaels@inet.uni2.dk
Mirza
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Svensson
L A
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Larsen
J N
JN
Gajhede
M
M
Ipsen
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H
D000485:Allergens / Q000737:chemistry* / Q000302:isolation & purification*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D052179:Antigens, Plant; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D051379:Mice; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D011058:Pollen / Q000737:chemistry* / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D010940:Plant Proteins; C061312:Bet v 1 allergen, Betula