Serological diagnosis of varicella-zoster virus in sera with antibody-capture enzyme-linked immunosorbent assay of IgM.
Evaluation was made of three enzyme-linked immunosorbent assay (ELISA) formats; varicella-zoster virus (VZV) indirect ELISA; VZV IgM capture using biotin and VZV IgM capture using peroxidase, for the detection of VZV-specific IgM antibodies in human sera. It was observed that there was no significant difference in sensitivity of detection using the three formats but there were important practical differences in the number of steps and hence time for assay completion between the three assay formats. All assays showed some cross-reactivity with sera containing anti-HSV1 antibodies.
Oladepo
D K
DK
Cortecs Diagnostics, Deeside, UK.
Klapper
P E
PE
Percival
D
D
Vallely
P J
PJ
D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D001710:Biotin / Q000378:metabolism; D002644:Chickenpox / Q000175:diagnosis* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006562:Herpes Zoster / Q000175:diagnosis* / Q000821:virology; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D009195:Peroxidase / Q000378:metabolism; D012698:Serologic Tests
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D001710:Biotin; D009195:Peroxidase