[Serum HBV DNA detected by polymerase chain reaction with dUTP/uracil-DNA glycosylase].
The ability of PCR reagent containing dUTP/uracil-DNA glycosylase for controlling carry-over contamination of PCR products was explored. All of 204 sera taken from hepatitis patients were used for HBV DNA detection by PCR with PCR-dUTP/UDG reagent in comparison with that without dUTP/UDG. The results showed that its efficiency of controlling contamination was excellent. At least, contamination of 100 ng PCR products was got rid of. The corresponding rate of HBV DNA detection by PCR-dUTP/UDG in combination with dot hybridization using digoxin-labeled HBV probe was as high as 89.32%, higher than that (81.45%) of PCR without dUTP/UDG plus dot hybridization(P < 0.05). It suggests that PCR-dUTP/UDG method could prevent PCR products from contaminating and increase accuracy and specificity of PCR amplification.
Liu
W E
WE
Department of Infectious Diseases, Xiangya Hospital, Hunan Medical University, Changsha.
Tan
D
D
Zhang
Z
Z
D045647:DNA Glycosylases; D004279:DNA, Viral / Q000097:blood*; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009699:N-Glycosyl Hydrolases; D016133:Polymerase Chain Reaction / Q000379:methods; D051981:Uracil-DNA Glycosidase
D004279:DNA, Viral; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase