Measurement of hyperforin a constituent of St. John's wort in plasma by high-performance liquid chromatography.
Hyperforin is a constituent of Hypericum perforatum extracts (St. John's wort, H. perforatum), which have antidepressant action. Hyperforin was extracted from plasma utilising a solid-phase extraction procedure. Chromatography was performed by isocratic reversed-phase high-performance liquid chromatography with UV end-point detection. The calibration curve was linear over the range 0.15-3 microg per ml of plasma. The sensitivity for hyperforin was 4.5 ng on-column. Mean inter- and intra-assay relative standard deviations over the range of the standard curve were less than 5%. The absolute recovery for hyperforin averaged 97.8%.
Chi
J D
JD
University of Oxford, Department of Psychiatry, Warneford Hospital, UK.
Franklin
M
M
D000818:Animals; D000928:Antidepressive Agents / Q000097:blood*; D001643:Bridged Bicyclo Compounds; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020902:Hypericum / Q000737:chemistry*; D008297:Male; D010696:Phloroglucinol / Q000031:analogs & derivatives; D010946:Plants, Medicinal; D051381:Rats; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013729:Terpenes / Q000097:blood
D000928:Antidepressive Agents; D001643:Bridged Bicyclo Compounds; D013729:Terpenes; D010696:Phloroglucinol; C001654:hyperforin