Studies on the callus cultures of Ginkgo biloba and its metabolites-ginkgolides.
The production of ginkgolides in callus culture of Ginkgo biloba was reported. The affection of some physical factors and chemical substances on the induction and growth of calli was also investigated. A biologically quantitative method (platelet aggregation induced by PAF) and HPLC were successfully used for the determination of Ginkgolides A and B in all kinds of callus cultures. The result showed that the content of Ginkgolides B in the callus cultures varies from 0.005% to 0.01%, which is one of the best results for the callus culture of G.biloba in the world.
Yu
R
R
Shenyang Pharmaceutical University, China.
Zhao
H
H
Zheng
Y
Y
Yao
X
X
Zhang
H
H
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D020441:Ginkgo biloba / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D046934:Ginkgolides; D007783:Lactones / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D010946:Plants, Medicinal; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors
D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D046934:Ginkgolides; D007783:Lactones; D010937:Plant Growth Regulators; D010972:Platelet Activating Factor; D015084:2,4-Dichlorophenoxyacetic Acid; C045856:ginkgolide B