title: cDNA-derived amino acid sequence of acetoacetyl-CoA synthetase from rat liver.
abstract: In order to examine the primary structure of acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, EC 6.2.1.16; AA-CoA synthetase), the cDNA clone encoding this enzyme has been isolated from the cDNA library which was prepared from the liver of rat fed a diet supplemented with 4% cholestyramine and 0.4% pravastatin for 4 days. Nucleotide sequence analysis of cloned cDNA revealed that AA-CoA synthetase of rat liver contains an open reading frame of 2019 nucleotides, and the deduced amino acid sequence (672 amino acid residues) bears 25.0 and 38.9% homologies with acetyl-CoA synthetases of Saccharomyces cerevisiae and Archaeoglobus fulgidus, respectively.
authors.affiliation: Department of Health Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Iwahori
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Nakamoto
authors.forename: M
authors.initials: M
authors.lastname: Iwama
authors.forename: T
authors.initials: T
authors.lastname: Fukui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002792:Cholestyramine Resin / Q000008:administration & dosage; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary; D004032:Diet; D005260:Female; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D017035:Pravastatin / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D002792:Cholestyramine Resin; D003066:Coenzyme A Ligases; C023747:acetoacetyl-CoA synthetase; D017035:Pravastatin