Two glycogen synthase activities associated with proteoglycogen in retina.
Glycogen synthase of bovine retina was found associated with the acid-insoluble and acid-soluble proteoglycogen fractions. The synthase associated with the acid-insoluble proteoglycogen precursor showed an 8-fold lower Km for UDP-glucose than the synthase associated with the acid-soluble fraction, and was inhibited by detergent. A short digestion with pronase resulted in conversion of the acid insoluble fraction into acid-soluble. The results lead us to postulate that the acid-insolubility of the proteoglycogen fraction and the association with retina membrane proposed before, is caused by glycogen synthase strongly associated to its polysaccharide moiety. The enlargement of the polysaccharide moiety during proteoglycogen biosynthesis, from glycogenin linked to a few 11 to 12 glucose units to the acid-insoluble proteoglycogen precursor (Mr 470,000) would be carried out, together with the branching enzyme, by the glycogen synthase showing a low Km for UDP-glucose. The glycogen synthase with the highest Km for UDP-glucose would participate in conversion of the precursor into mature acid-soluble proteoglycogen.
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Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), UNC-CONICET, Departamento de Química Biológica Dr. Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. jcurtino@dqbfcq.uncor.edu
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D000818:Animals; D002417:Cattle; D003902:Detergents / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005964:Glucosyltransferases; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011402:Pronase / Q000378:metabolism; D012160:Retina / Q000201:enzymology*; D012995:Solubility; D014238:Trichloroacetic Acid; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D003902:Detergents; D004791:Enzyme Inhibitors; D006023:Glycoproteins; D011134:Polysaccharides; C051887:glycogenin; D014238:Trichloroacetic Acid; D005964:Glucosyltransferases; D006006:Glycogen Synthase; D011402:Pronase; D014532:Uridine Diphosphate Glucose