No involvement of lipid mediators in a guinea pig model of ultrasonically nebulized distilled water-induced bronchoconstriction.
An inhalation of ultrasonically nebulized distilled water (UNDW) induces bronchoconstriction only in asthmatics, but the mechanism underlying the response is not fully understood. We have reported that bronchoconstriction occurs immediately after UNDW is inhaled 20 min after an antigen challenge in guinea pigs. Our aim was to examine the role of lipid mediators in this response. Passively sensitized guinea pigs were anesthetized and artificially ventilated. A sulfidopeptide leukotriene receptor antagonist, KCA-757, and platelet-activating factor antagonists, Y-24180 and E6123, were administered i.v. 15 min after an aerosolized antigen challenge, and UNDW was inhaled 5 min later. KCA-757, Y-24180, or E6123 did not, significantly alter the UNDW-induced bronchoconstriction. Together with our previous data that thromboxane A2 receptor antagonists did not influence the UNDW-induced bronchoconstriction, the present results suggest that lipid mediators are not involved in the UNDW-induced bronchoconstriction in our guinea pig model.
Fujimura
M
M
Third Department of Internal Medicine, Kanazawa University School of Medicine, Japan. fujimura@med3.m.kanazawa-u.ac.jp
Ishiura
Y
Y
Amemiya
T
T
Myou
S
S
Matsuda
T
T
D000818:Animals; D016084:Bronchoconstriction / Q000187:drug effects; D006168:Guinea Pigs; D020024:Leukotriene Antagonists; D008055:Lipids / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D009330:Nebulizers and Vaporizers; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D014465:Ultrasonics; D014867:Water / Q000008:administration & dosage
D020024:Leukotriene Antagonists; D008055:Lipids; D010972:Platelet Activating Factor; D014867:Water