Determination of histamine in tomatoes by capillary electrophoresis.
An improved capillary electrophoresis assay for histamine in crude extracts was developed and used to determine histamine levels in a selection of tomato fruits and pastes. Performance in terms of reproducibility and sensitivity was optimized by use of a high sensitivity detector flow cell, sodium hydroxide rinses, and a voltage gradient. The method was linear down to 0.2 microgram/mL (signal-to-noise ratio = 4:1), which was below the endogenous level in all samples.
Bolygo
E
E
Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire, UK.
Cooper
P A
PA
Jessop
K M
KM
Moffatt
F
F
D019075:Electrophoresis, Capillary; D005506:Food Contamination; D006632:Histamine / Q000032:analysis*; D018551:Solanum lycopersicum / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012972:Sodium Hydroxide; D013056:Spectrophotometry, Ultraviolet
D012972:Sodium Hydroxide; D006632:Histamine