title: Expression and regulation of melanocortin receptor-5 (MC5-R) in the bovine adrenal cortex.
abstract: Among the five members of the melanocortin receptor (MC-R) family, MC2 and MC5 are expressed in peripheral tissues. The receptor MC2 (ACTH receptor) almost exclusively expressed in the adrenal cortex whereas MC5-R is expressed in several organs including the adrenal cortex. Both receptors bind ACTH and activate adenylate cyclase. The aim of this work was to study the spatial distribution of MC5-R among the different zones of the bovine adrenal cortex and to analyze the regulation of its expression by its own ligands, ACTH and alpha-MSH and by angiotensin II (AII). Using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and RNase protection assay, MC5-R was detected only in the glomerulosa zone whereas MC2-R was present in both glomerulosa and fasciculata zones of adult adrenal cortex. Treatments by ACTH, alpha-MSH, or AII increased the MC5-R mRNA level in glomerulosa cells by factors 7, 5, and 4.5, respectively. However, although potentially regulated by hormones, MC5-R is expressed at a level at least 100 times less than MC2-R, suggesting that MC5-R expression might only be at trace levels in grown adults, but could be much higher during embryogenesis.
authors.affiliation: INSERM Unité 244, CEA, Department of Molecular and Structural Biology, Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Liakos
authors.forename: E M
authors.initials: EM
authors.lastname: Chambaz
authors.forename: J J
authors.initials: JJ
authors.lastname: Feige
authors.forename: G
authors.initials: G
authors.lastname: Defaye
mesh_terms: D000302:Adrenal Cortex / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044103:Receptor, Melanocortin, Type 2; D018043:Receptors, Corticotropin / Q000235:genetics* / Q000378:metabolism*; D044101:Receptors, Melanocortin; D014018:Tissue Distribution; D000521:alpha-MSH / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D044103:Receptor, Melanocortin, Type 2; D018043:Receptors, Corticotropin; D044101:Receptors, Melanocortin; C086971:melanocortin 5 receptor; D000804:Angiotensin II; D000521:alpha-MSH; D000324:Adrenocorticotropic Hormone