The yeast nuclear pore complex: composition, architecture, and transport mechanism.
An understanding of how the nuclear pore complex (NPC) mediates nucleocytoplasmic exchange requires a comprehensive inventory of the molecular components of the NPC and a knowledge of how each component contributes to the overall structure of this large molecular translocation machine. Therefore, we have taken a comprehensive approach to classify all components of the yeast NPC (nucleoporins). This involved identifying all the proteins present in a highly enriched NPC fraction, determining which of these proteins were nucleoporins, and localizing each nucleoporin within the NPC. Using these data, we present a map of the molecular architecture of the yeast NPC and provide evidence for a Brownian affinity gating mechanism for nucleocytoplasmic transport.
Rout
M P
MP
The Rockefeller University, New York, NY 10021, USA. rout@rockvax.rockefeller.edu
Aitchison
J D
JD
Suprapto
A
A
Hjertaas
K
K
Zhao
Y
Y
Chait
B T
BT
D001692:Biological Transport; D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins