[Morphometric analysis of nuclear variations in normal and neoplastic mammary ductal cells].
Morphometry is a method to detect changes in a variety of tissues through quantitative elements. The purpose of this study was to examine several nuclear morphologic characteristics in normal and neoplastic mammary ductal cells using a multivariable method and expression of estrogen receptors by immunohistochemical techniques. A total of 1879 nuclei were examined by a computerized program, following the detection of estrogen receptors. Nuclear area, perimeter, diameter, maximal and minimal radio were obtained in 439 normal ductal nuclei. The mean nuclear area was 14.45 with a range between 10.88 and 17.90. Variables showed adequate statistical correlation (r > 0.5). A total of 1440 neoplastic nuclei were classified as grades I, II and III, and a statistical significative difference was found between these three groups. We conclude that the nuclear area is a reliable variable for statistical correlation being the ductal nuclei anisotropic objects.
Parada
D
D
Unidad de Investigación, Facultad de Medicina, Universidad Central de Venezuela. parada@cantv.net
Farías
R M
RM
García-Tamayo
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001940:Breast / Q000166:cytology*; D001943:Breast Neoplasms / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology*; D005260:Female; D006801:Humans; D008875:Middle Aged