[Application of proliferating cell nuclear antigen in the study of human trabecular cell proliferation].
OBJECTIVE
To evaluate the application of proliferating cell nuclear antigen (PCNA) in the study of human trabecular cell proliferation.


METHODS
Immunohistochemical technique was used to observe the PCNA expressive level in the fourth passage of human trabecular meshwork, the effects of different concentrations of epinephrine, dexamethasone and epidermal growth factor on the level were also investigated, the results were compared with that of the normal cells cultured at the same time, and analyzed by a graphic device operation system in a computer.


RESULTS
A stable proliferating curve was obtained according to the normal cell PCNA level, by which we could choose the best opportunity of drug application. Epinephrine and dexamethasone were found to significantly inhibit cell proliferation, while epidermal growth factor (EGF) could promote the proliferation.


CONCLUSIONS
PCNA is considered to be a useful agent to observe the process of cell proliferation. The above mentioned methods are beneficial to the investigations of biochemical characteristics of trabecular meshwork cells and the pathogenic mechanisms of open-angle glaucoma.
Dai
W
W
Department of Ophthalmology, First Hospital, Beijing Medical University.
Li
M
M
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D014129:Trabecular Meshwork / Q000166:cytology / Q000276:immunology*
D018809:Proliferating Cell Nuclear Antigen; D004815:Epidermal Growth Factor; D003907:Dexamethasone; D004837:Epinephrine