Determination of dihydroergotamine in human plasma by high-performance liquid chromatography with fluorescence detection.
Dihydroergotamine, a 5-hydroxytryptamine antagonist, is used for the treatment of vascular headaches. A high-performance liquid chromatography assay with fluorescence detection is described for the determination of dihydroergotamine in plasma. The assay was validated over the concentration range 0.1-10 ng/ml plasma and applied to the analysis of plasma samples from subjects treated intramuscularly and intranasally with 2 mg of dihydroergotamine.
Murday
M
M
Shire Laboratories Inc., Rockville, MD 20850, USA.
McLean
A M
AM
Slaughter
E
E
Couch
R A
RA
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004087:Dihydroergotamine / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D004087:Dihydroergotamine