Capillary electrophoretic determination of sanguinarine and chelerythrine in plant extracts and pharmaceutical preparations.
Capillary electrophoresis was employed to determine the principal quaternary benzo[c]phenanthridine alkaloids, sanguinarine and chelerythrine, in two plant extracts and one oral hygiene product. Phosphate-Tris buffer of pH 2.5 was used as a background electrolyte, limits of detection were 3 micromol/l(-1) (sanguinarine) and 2.4 micromol,l(-1) (chelerythrine) using UV detection at 270 nm. The method, which correlated well with HPLC, is suitable for serial determination of sanguinarine and chelerythrine in plant products and pharmaceuticals.
Sevcík
J
J
Department of Analytical Chemistry, Palacký University, Olomouc, Czech Republic.
Vicar
J
J
Ulrichová
J
J
Válka
I
I
Lemr
K
K
Simánek
V
V
D000470:Alkaloids / Q000032:analysis*; D053119:Benzophenanthridines; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D007546:Isoquinolines; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010617:Phenanthridines / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000470:Alkaloids; D053119:Benzophenanthridines; D007546:Isoquinolines; D004364:Pharmaceutical Preparations; D010617:Phenanthridines; D010936:Plant Extracts; C005705:sanguinarine; C016299:chelerythrine