Identification and differentiation of the nine African horse sickness virus serotypes by RT-PCR amplification of the serotype-specific genome segment 2.
This paper describes the first RT-PCR for discrimination of the nine African horse sickness virus (AHSV) serotypes. Nine pairs of primers were designed, each being specific for one AHSV serotype. The RT-PCR was sensitive and specific, providing serotyping within 24 h. Perfect agreement was recorded between the RT-PCR and virus neutralization for a coded panel of 56 AHSV reference strains and field isolates. Serotyping was achieved successfully with live and formalin-inactivated AHSVs, with isolates of virus after low and high passage through either tissue culture or suckling mouse brain, with viruses isolated from widely separated geographical areas and with viruses isolated up to 37 years apart. Overall, this RT-PCR provides a rapid and reliable method for the identification and differentiation of the nine AHSV serotypes, which is vital at the start of an outbreak to enable the early selection of a vaccine to control the spread of disease.
Sailleau
C
C
Agence Française de Sécurité Sanitaire des Aliments - Alfort, Laboratoire Central de Recherches Vétérinaires, 22 rue Pierre Curie, 94703 Maisons-Alfort, France. c.sailleau@alfort.afssa.fr
Hamblin
C
C
Paweska
J T
JT
Zientara
S
S
D000355:African Horse Sickness / Q000517:prevention & control / Q000821:virology; D000356:African Horse Sickness Virus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D016679:Genome, Viral; D006736:Horses; D051379:Mice; D009500:Neutralization Tests; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods* / Q000706:statistics & numerical data; D014765:Viral Vaccines / Q000302:isolation & purification
D017931:DNA Primers; D014765:Viral Vaccines