High throughput on-line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum.
The feasibility of high throughput on-line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 seconds for the entire, fully automated assay. The assay proved to be linear from 1 to 1000 ng/mL. Cartridges packed with small sorbent particles functioned both as SPE cartridges and as short analytical columns.
Schellen
A
A
Spark Holland BV, P.O. Box 388, 7800 AJ Emmen, The Netherlands.
Ooms
B
B
van Gils
M
M
Halmingh
O
O
van der Vlis
E
E
van de Lagemaat
D
D
Verheij
E
E
D001774:Blood Chemical Analysis / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation*; D017239:Paclitaxel / Q000097:blood*; D015203:Reproducibility of Results; D013997:Time Factors
D017239:Paclitaxel