Apparatus and method for interfacing capillary electrophoresis and chemiluminescence nitrogen detection for the analysis of nitrogen-containing compounds.
We have developed an interface that allows the specific detection of nitrogen-containing compounds by using a chemiluminescence nitrogen detector. The feasibility of using this interface was demonstrated by separating and detecting two nitrogen-containing compounds, p-aminosalicylic acid and L-phenylalanine. Although baseline separation was achieved, the theoretical plates were lower when compared to UV detection (25000 vs. approximately 85000). A sensitivity of 75 ng (approximately 500 pmol) per injection was achieved with this system which is adequate for pharmaceutical and biotech applications.
Mohammad
A A
AA
Department of Pathology, University of Texas-Medical Branch, Galveston 77555-0551, USA.
Fujinari
E
E
Okorodudu
A O
AO
Petersen
J R
JR
D010131:Aminosalicylic Acid / Q000032:analysis / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods*; D008163:Luminescent Measurements; D009584:Nitrogen / Q000032:analysis*; D010649:Phenylalanine / Q000032:analysis / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D010649:Phenylalanine; D010131:Aminosalicylic Acid; D009584:Nitrogen