Histone H1 variants play individual roles in transcription regulation in the DT40 chicken B cell line.
Thirty-nine of the 44 chicken histone genes are located in a major gene cluster of 110 kb, the others being distributed in four separate regions. All 6 H1 genes, which are present in the cluster and encode different variants, are expressed in the DT40 chicken B cell line, at levels ranging from about 5 to 40%. To clarify differences in the natures of these H1 variants, using gene-targeting techniques, we generated a series of DT40 mutants, which are devoid of each of the 5 H1 genes, respectively. Analyses of six H1-deficient mutants, comprising the latter five and a previously generated H1-deficient mutant, revealed that the protein patterns on 2D-PAGE were definitely different from each other, indicating that each H1 variant plays an individual role in the transcription regulation of specific genes in DT40 cells.
Takami
Y
Y
Department of Biochemistry, Miyazaki Medical College, Kihara, Miyazaki, Kiyotake, 889-1692, Japan.
Nishi
R
R
Nakayama
T
T
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002460:Cell Line; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D006657:Histones / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D006657:Histones; D012333:RNA, Messenger