Immunohistochemical localization of neurocalcin in human sensory neurons and mechanoreceptors.
The localization of neurocalcin in the developing and adult human peripheral nervous system (dorsal root and sympathetic ganglia (DRG, SG), and enteric nervous system (ENS)) was investigated using immunohistochemistry. A subpopulation of large-sized neurons in DRG of 9 and 12 weeks old embryos showed immunoreactivity (IR), whereas the sympathetic ganglia or enteric neurons did not. In adults, neurocalcin IR was restricted to a subpopulation of large (13%) and intermediate (15%) sized neurons in DRG. The protein was also found in muscular (67%) and cutaneous (12%) nerve fibers, as well as in the axons supplying muscular (muscle spindles, Golgi's tendon organs, and perimysial Pacinian corpuscles) and cutaneous (Meissner's but not Pacinian corpuscles) mechanoreceptors, as well as motor end-plates. Present results demonstrate that neurocalcin in both developing and adult humans can be used as a specific marker for a subpopulation of sensory neurons coupled to proprioception and touch, and for axons of motoneurons forming motor end-plates.
Galeano
R M
RM
Departamento de Morfología y Biología Celular, Universidad de Oviedo, Facultad de Medicina, Spain.
Germanà
A
A
Vázquez
M T
MT
Hidaka
H
H
Germanà
G
G
Vega
J A
JA
D000328:Adult; D001369:Axons / Q000648:ultrastructure; D002135:Calcium-Binding Proteins / Q000032:analysis*; D004622:Embryo, Mammalian; D005727:Ganglia, Spinal / Q000166:cytology* / Q000196:embryology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008465:Mechanoreceptors / Q000166:cytology*; D008875:Middle Aged; D009045:Motor Endplate / Q000648:ultrastructure; D009046:Motor Neurons / Q000166:cytology; D018482:Muscle, Skeletal / Q000166:cytology*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051596:Neurocalcin; D009475:Neurons, Afferent / Q000166:cytology*; D011434:Proprioception; D044169:Receptors, Calcium-Sensing; D012160:Retina / Q000166:cytology / Q000196:embryology; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology; D014110:Touch; D012668:Trigeminal Ganglion / Q000166:cytology* / Q000196:embryology
D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing