title: Anti-phospholipase A2 and anti-inflammatory activity of Santolina chamaecyparissus.
abstract: The activity of the Santolina chamaecyparissus methanol extract was tested against the phospholipase A2 (PLA2)-induced mouse paw edema and in vitro inhibition of PLA2 activity. After fractionation, only the dichloromethane extract was active against the PLA2 in vitro test. In addition, it reduced the edema induced by arachidonic acid, and by 12-O-tetradecanoylphorbol-13-acetate in a multidose test. After chromatography on silicagel and gel filtration on Sephadex, and using an in vitro anti-PLA2 assay-guided process, we have isolated and identified from the dichloromethane extract the flavone nepetin and four sesquiterpenes.
authors.affiliation: Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Burjassot, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Sala
authors.forename: M C
authors.initials: MC
authors.lastname: Recio
authors.forename: R M
authors.initials: RM
authors.lastname: Giner
authors.forename: S
authors.initials: S
authors.lastname: Máñez
authors.forename: J L
authors.initials: JL
authors.lastname: Ríos
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; D012717:Sesquiterpenes; D010741:Phospholipases A; D054467:Phospholipases A2