Genetic restrictions in olive pollen allergy.
BACKGROUND
The major antigen of olive tree pollen, Ole e 1, produces an IgE response restricted by DQ2.


OBJECTIVE
Our purpose was to further analyze the genetic restrictions associated with IgE and IgG antibodies against Ole e 1 and IgE against the recently described antigen Ole e 3.


METHODS
Twenty-two nuclear olive pollen-allergic families (n = 88) were selected. DRB1 and DQB1, TCR-Valpha 8.1, the high-affinity receptor of IgE (FcepsilonRI-beta) Rsa I exon 7 and intron 2 and TNF-beta (LTalpha-Nco I) polymorphisms were determined by PCR and analyzed for association with allergic traits by the multiallelic transmission disequilibrium test.


RESULTS
Significant associations were found among HLA-DQB1*0201 (n = 29) and high levels of IgG (P =.023) and IgE (P =.0136) antibodies to Ole e 1 and with IgE specific to Ole e 3 (P =.0368). DRB1*0701 was associated with high levels of total serum IgE (P =.04) and IgG against Ole e 1 (P =.025). The FcepsilonRI-beta Rsa I exon 7, allele 1 (n = 39), was associated with high levels of total serum IgE (P =. 01), IgE antibodies against Olea europaea extract (P =.004), and specific antibodies to Ole e 1, IgG (P =.04), and IgE (P =.006). The FcepsilonRI-beta Rsa I intron 2, allele 2 (n = 33), was associated with IgE antibodies to O europaea extract (P =.003) and specific antibodies to Ole e 1, IgG (P =.025), and IgE (P =.05).


CONCLUSIONS
We found a new association between IgE antibody response to Ole e 3 and DQB1*0201 and verified the previously reported association between Ole e 1-specific response and DQB1*0201. Also, the association between FcepsilonRI-beta and IgE antibodies against Ole e 1 was demonstrated.
Cardaba
B
B
Immunology Department, Fundacion Jimenez Diaz, Madrid, Spain.
Cortegano
I
I
Florido
F
F
Arrieta
I
I
Aceituno
E
E
del Pozo
V
V
Gallardo
S
S
Rojo
M
M
Palomino
P
P
Lahoz
C
C
D000293:Adolescent; D000485:Allergens; D052179:Antigens, Plant; D002135:Calcium-Binding Proteins; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D008233:Lymphotoxin-alpha / Q000235:genetics / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010940:Plant Proteins / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011110:Polymorphism, Genetic / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology; D014197:Trees / Q000276:immunology*
D000485:Allergens; D052179:Antigens, Plant; D002135:Calcium-Binding Proteins; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D008233:Lymphotoxin-alpha; C102722:OLE3 protein, Olea europaea; C081946:Ole e I protein, Olea europaea; D010940:Plant Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D017455:Receptors, IgE; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases