Alveolar macrophage cytokine production in response to air particles in vitro: role of endotoxin.
The interaction of air particles and alveolar macrophages (AMs) may result in the release of proinflammatory cytokines. Normal mouse AMs were treated with concentrated air particle (CAPs) suspensions in vitro. After 5 h, cytokine release [macrophage inflammatory protein-2 (MIP-2) and tumor necrosis factor-alpha (TNF-alpha)] and phagocytosis of ambient air particles were measured. CAPs samples collected from urban air (Boston) on different days were used. The CAPs samples and their soluble and solid components caused significant MIP-2 and TNF-alpha production. Variability in the potency of samples collected on different days was observed. Trace endotoxin was measured in CAPs samples (EU/mg: 2.3 +/- 0.7, mean +/- SE, n = 10). A majority of biologic activity (cytokine induction) and endotoxin content was associated with the solid components. Neutralization of endotoxin by polymyxin B abrogated >80% of TNF-alpha induction by CAPs samples, but inhibited MIP-2 production by only approximately 40%. The trace endotoxin present in CAPs caused much more MIP-2 production than predicted by concentration alone (28 +/- 8-fold increase, n = 9), indicating synergistic interaction with other AM-activating components of the particles. Data suggest that low levels of endotoxin may interact with air particles to activate lung macrophages.
Ning
Y
Y
Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Imrich
A
A
Goldsmith
C A
CA
Qin
G
G
Kobzik
L
L
D000393:Air Pollutants / Q000032:analysis / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D054426:Chemokine CXCL2; D004731:Endotoxins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D015846:Monokines / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects; D013535:Suspensions; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism
D000393:Air Pollutants; D054426:Chemokine CXCL2; D004731:Endotoxins; D015846:Monokines; D013535:Suspensions; D014409:Tumor Necrosis Factor-alpha