[Diagnostic value of CD44 splice variants in urine exfoliated cells of bladder cancer].
To study the early and non-invasive diagnostic value of CD44 splice variants in urine exfoliated cells of bladder cancer. We used reverse transcriptionpolymerase chain resection (RT-PCR) and southern blot hybridization to detect CD44 splice variants in exfoliated cells in 40 urine samples (20 bladder cancers, 20 non-neoplastic controls), and compared with the results of urine cytology on the same set of samples. 90% (18/20) of the urine samples of bladder cancer showed overexpression of CD44 splice variants while none of the 20 controls did so. This method not only has a sensitivity of 90% (18/20) which was much higher than that of 65% (13/20) by using urine cytology, but also is non-invasive and comfortable. The results suggest that CD44 splice variants in exfoliated cells in urine samples are a new tumor marker for early and non-invasive diagnosis of bladder cancer.
Wu
Z
Z
Department of Urology, Huashan Hospital, Shanghai Medical University.
Zhang
Y
Y
Liu
D
D
D014408:Biomarkers, Tumor; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics; D005091:Exons; D014644:Genetic Variation; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis / Q000235:genetics*; D012326:RNA Splicing; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics
D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors