An integrin-targeted non-viral vector for pulmonary gene therapy.
Gene therapy offers potential for the treatment of severe respiratory diseases. However, the vectors which are currently available have drawbacks limiting their therapeutic application. Here we report on an integrin-targeted, non-viral gene delivery system for pulmonary gene transfer. We demonstrate that this vector can deliver the lacZ reporter gene to the lung, transfecting bronchial epithelium and parenchymal cells with similar efficiency to an adenoviral vector and with greater efficiency than a cationic liposome. In addition, vector administration can be repeated leading to further gene expression without inducing inflammation. The advantages of this novel gene delivery system provide considerable potential for targeted gene therapy in vivo. Gene Therapy (2000) 7, 393-400.
Jenkins
R G
RG
Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, Royal Free and University College Medical School, University College London, Rayne Institute, London, UK.
Herrick
S E
SE
Meng
Q H
QH
Kinnon
C
C
Laurent
G J
GJ
McAnulty
R J
RJ
Hart
S L
SL
D000818:Animals; D018390:Gene Targeting; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D007150:Immunohistochemistry; D016023:Integrins / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics; D008168:Lung / Q000378:metabolism; D008171:Lung Diseases / Q000628:therapy; D008297:Male; D011014:Pneumonia / Q000209:etiology / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D001616:beta-Galactosidase / Q000378:metabolism
D016023:Integrins; D001616:beta-Galactosidase