Identification of a melanoma antigen, PRAME, as a BCR/ABL-inducible gene.
In order to elucidate molecular events in BCR/ABL-induced transformation, we adopted a polymerase chain reaction (PCR)-based technique of differential display and compared mRNA expression in human factor-dependent cells, TF-1, with that in factor-independent cells, ID-1, which were established from TF-1 cells by transfection of BCR/ABL. Cloning and sequencing of a gene which was upregulated in ID-1 cells revealed that the gene was identical to a melanoma antigen, PRAME. Our present study demonstrated that PRAME was markedly expressed in primary leukemic cells with chronic myeloid leukemia (CML) in blastic crisis and Philadelphia (Ph)+-acute lymphoblastic leukemia (ALL), in which BCR/ABL played an important role as a pathogenic gene. Moreover, comparison of PRAME expression among CD34+ cells with CML in blastic, accelerated, and chronic phases revealed a higher expression in CML in advanced phases. Thus PRAME was considered to be a good candidate for a marker of Ph+-leukemic blast cells as well as a new target antigen of leukemic blast cells that cytotoxic T cells can recognize.
Watari
K
K
Department of Hematology-Oncology, Institute of Medical Science, University of Tokyo, Japan. kiyoshi@ims.u-tokyo.ac.jp
Tojo
A
A
Nagamura-Inoue
T
T
Nagamura
F
F
Takeshita
A
A
Fukushima
T
T
Motoji
T
T
Tani
K
K
Asano
S
S
D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary; D016044:Fusion Proteins, bcr-abl / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology / Q000473:pathology; D008545:Melanoma / Q000276:immunology*; D008969:Molecular Sequence Data; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D018076:DNA, Complementary; C404944:PRAME protein, human; D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl