Genomic organization and transcriptional analysis of the human genes coding for caveolin-1 and caveolin-2.
Caveolin-1 and caveolin-2 are related proteins involved in the biogenesis of caveolae. The corresponding genes in humans (CAV and CAV2, respectively), have been mapped to a common locus in chromosome 7q31.1, and are possible candidates for the tumor suppressor gene postulated in this region. Here, we show that CAV and CAV2 are independent transcriptional units lying in the same orientation, with CAV2 centromeric and about 17kb upstream to CAV. The two genes have similar tissue expression patterns. Alternative termination/polyadenylation generates two CAV2 mRNAs. Multiple transcriptional start sites spanning 35bp upstream from the CAV2 ATG are detected by 5' RACE, consistent with a TATA-less promoter predicted by sequence analysis. The CAV2 promoter region contains two SRE-like boxes resembling those described in the CAV promoter and proposed to link transcription to intracellular cholesterol levels. However, exogenous sterols had only minor effects on CAV and CAV2 RNA levels in HeLa cells, suggesting that SREBPs are not sufficient to regulate caveolin transcription.
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DIBIT-San Raffaele Scientific Institute, 20132, Milan, Italy.
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Mancini
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D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology; D005091:Exons; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D019169:Jurkat Cells; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D051780:Sterol Regulatory Element Binding Protein 1; D014018:Tissue Distribution; D014157:Transcription Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
C495540:CAV1 protein, human; D022762:CCAAT-Enhancer-Binding Proteins; D051242:Caveolin 1; D051243:Caveolin 2; D022461:Caveolins; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C497387:SREBF1 protein, human; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; D004247:DNA