title: Identification and characterization of functional subunits of Clostridium botulinum type A progenitor toxin involved in binding to intestinal microvilli and erythrocytes.
abstract: Clostridium botulinum type A hemagglutinin-positive progenitor toxin consists of three distinct components: neurotoxin (NTX), hemagglutinin (HA), and non-toxic non-HA (NTNH). The HA consists of four subcomponents designated HA1, 2, 3a and 3b. By employing purified toxin and GST-fusion proteins of each HA subcomponent, we found that the HA-positive progenitor toxin, GST-HA1 and GST-HA3b bind to human erythrocytes and microvilli of guinea pig upper small intestinal sections. The HA-positive progenitor toxin and GST-HA1 bind via galactose moieties, GST-HA3b binds via sialic acid moieties. GST-2 and GST-3a showed no detectable binding.
authors.affiliation: Department of Bacteriology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Nomura
authors.forename: J
authors.initials: J
authors.lastname: Sasaki
authors.forename: J C
authors.initials: JC
authors.lastname: Marvaud
authors.forename: M R
authors.initials: MR
authors.lastname: Popoff
authors.forename: S
authors.initials: S
authors.lastname: Kozaki
authors.forename: K
authors.initials: K
authors.lastname: Oguma
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A / Q000737:chemistry* / Q000378:metabolism; D003014:Clostridium botulinum; D004912:Erythrocytes / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006168:Guinea Pigs; D006388:Hemagglutinins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006388:Hemagglutinins; C088300:NTNH protein, Clostridium botulinum; D011993:Recombinant Fusion Proteins; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; D019158:N-Acetylneuraminic Acid; D005690:Galactose