First evidence and characterization of an uncoupling protein in fungi kingdom: CpUCP of Candida parapsilosis.
An uncoupling protein (UCP) was identified in mitochondria from Candida parapsilosis (CpUCP), a non-fermentative parasitic yeast. CpUCP was immunodetected using polyclonal antibodies raised against plant UCP. Activity of CpUCP, investigated in mitochondria depleted of free fatty acids, was stimulated by linoleic acid (LA) and inhibited by GTP. Activity of CpUCP enhanced state 4 respiration by decreasing DeltaPsi and lowered the ADP/O ratio. Thus, it was able to divert energy from oxidative phosphorylation. The voltage dependence of electron flux indicated that LA had a pure protonophoretic effect. The discovery of CpUCP proves that UCP-like proteins occur in the four eukaryotic kingdoms: animals, plants, fungi and protists.
Jarmuszkiewicz
W
W
Department of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland.
Milani
G
G
Fortes
F
F
Schreiber
A Z
AZ
Sluse
F E
FE
Vercesi
A E
AE
D002175:Candida / Q000187:drug effects / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007473:Ion Channels; D019787:Linoleic Acid / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D010085:Oxidative Phosphorylation / Q000187:drug effects; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1; D006160:Guanosine Triphosphate; D019787:Linoleic Acid