title: Simultaneous determination of eprinomectin, moxidectin, abamectin, doramectin, and ivermectin in beef liver by LC with fluorescence detection.
abstract: Eprinomectin, moxidectin, abamectin, doramectin, and ivermectin are drugs used to control parasitic infections in both meat-producing and nonmeat-producing animals. A number of analytical methods are available to analyze these anthelmintic drugs individually. A multiresidue screening method was developed for these drugs; however, the initial attempt to derivatize eprinomectin following the method published by Merck scientists was unsuccessful because the eprinomectin derivatization reaction was temperature- and time-dependent. The optimum time and temperature for the completion of eprinomectin derivatization were 90 min and 65 degrees C, respectively, without appreciable effect on the remaining 4 drugs. Beef liver samples were fortified with 0, 25, 50, and 100 ppb mixed standards of eprinomectin, moxidectin, abamectin, doramectin, and ivermectin. Each set of 4 levels of recoveries was repeated 10 times with all 5 compounds. The average of 10 recoveries of 5 compounds at all 4 levels of fortification was > 70%; the coefficient of variation was < 20%.
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, Athens, GA 30604, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Ali
authors.forename: T
authors.initials: T
authors.lastname: Sun
authors.forename: G E
authors.initials: GE
authors.lastname: McLeroy
authors.forename: E T
authors.initials: ET
authors.lastname: Phillippo
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis; D000969:Antinematodal Agents / Q000032:analysis; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D018942:Macrolides; D011786:Quality Control; D013050:Spectrometry, Fluorescence
chemical_list: D000871:Anthelmintics; D000900:Anti-Bacterial Agents; D000969:Antinematodal Agents; D018942:Macrolides; C048324:abamectin; D007559:Ivermectin; C101434:eprinomectin; C084101:doramectin; C027837:moxidectin