Monoclonal antibody inhibition of Neospora caninum tachyzoite invasion into host cells.
Monoclonal antibodies were produced against Neospora caninum tachyzoites to identify antigens which may play a role during invasion of host cells. Confocal laser microscopy showed that most antigens recognised by the mAb were located on the surface, but one mAb, 1A5, reacted to the apical end of the parasite. Some mAbs, which recognised 70, 42 and 36kDa parasite proteins, significantly inhibited the invasion of the parasite in vitro. The mAbs which recognised 42 and 36kDa parasite protein, reacted with Nc-p43 and Nc-p36 expressed by vaccinia virus and Escherichia coli, respectively. These results suggest that a 70kDa protein, Nc-p43 and Nc-p36 are involved in the invasion of the parasite into host cells.
Nishikawa
Y
Y
The Research Center for Protozoan Molecular Immunology, Obihiro University, Inadacho, Obihiro, Japan.
Xuan
X
X
Nagasawa
H
H
Igarashi
I
I
Fujisaki
K
K
Otsuka
H
H
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000913:Antibodies, Protozoan / Q000494:pharmacology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000276:immunology* / Q000472:pathogenicity*; D015800:Protozoan Proteins / Q000276:immunology*; D014774:Virulence / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C110716:p43 protein, Neospora caninum; C117858:tachyzoite surface antigen p36 protein, Neospora caninum