Hypotensive effect of tenuifolic saponin and its mechanism.
OBJECTIVE
To study the effect of tenuifolic saponin (TS) on arterial pressure.


METHODS
Mean arterial pressure (MAP) was recorded from left carotid artery in rat which was anesthetized with urethane and then injected i.v. gtt with a transfusion of NaCl 0.15 mol.L-1. Systolic blood pressure (SBP) of conscious rat and renovascular hypertensive rat (RVHR) was measured by tail cuff method.


RESULTS
TS 2, 4, 8 mg.kg-1 i.v., 20 and 40 mg.kg-1 i.g. reduced the MAP by 31%, 37%, 50%, 21%, and 31%, respectively. Bilateral vagotomy plus atropine (Atr) i.v., or pretreatment with diphenhydramine hydrochloride (Dip) failed to influence TS effect. Lack of effect of TS on carotid-occlusion-induced- or epinephrine (Epi)-induced-hypertensive response was found. SBP in conscious rat and RVHR was suppressed, highest by 38.0% and 26.8% at 60 and 90 min, maintaining at least 2 and 3 h, respectively, after i.g. TS 40 mg.kg-1.


CONCLUSIONS
TS reduced the arterial pressure, not related to vagus excitation, ganglionic blockade, and peripheral alpha-adrenergic-, M-cholinergic-, and H1-receptors.
Peng
W D
WD
Department of Pharmacy, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China. pwd@gz.col.com.cn
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects*; D005260:Female; D006978:Hypertension, Renovascular / Q000503:physiopathology*; D008297:Male; D051379:Mice; D031801:Polygala; D051381:Rats; D012503:Saponins / Q000494:pharmacology*; D014628:Vagotomy
D000959:Antihypertensive Agents; D012503:Saponins; C407311:tenuifolic saponin