title: [Detection of 17-ketosteroid and 17-hydroxycorticosteroid in urine after separation and collection by foam rubber].
abstract: Using foam rubber 17-ketosteroid (17-KS) and 17-hydroxycorticosteroid (17-OHCS) were separated and collected in urine. 17-KS and 17-OHCS were eluted down from the foam rubber with alcohol, colour reaction can be respectively produced by m-dinitrobenzene and phenyhydrazine hydrocholoride, then both of them were detected by spectro-photometry. The standard deviation of this method was +/- 0.032, and its minimum detectable concentration was 0.01 microgram.ml-1. The recovery rate of 17-KS and 17-OHCS estimation was from 99.0% to 101.0%, with co-efficients of variation less than 6.23%.
authors.affiliation: Central Laboratory, Xiangya Hospital, Hunan Medical University, Changsha.
authors.forename: Z
authors.initials: Z
authors.lastname: Jing
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Fu
mesh_terms: D015065:17-Hydroxycorticosteroids / Q000652:urine*; D015068:17-Ketosteroids / Q000652:urine*; D005260:Female; D006801:Humans; D008297:Male; D012016:Reference Values; D013053:Spectrophotometry / Q000379:methods
chemical_list: D015065:17-Hydroxycorticosteroids; D015068:17-Ketosteroids