Engagement of the scavenger receptor is not responsible for beta-amyloid stimulation of monocytes to a neurocytopathic state.
Experiments were performed to determine if scavenger receptors (SRs) play a role in amyloid beta (Abeta) stimulation of peripheral blood monocyte (PBM) neurotoxicity. Results indicate that Abeta does not block binding of the SR ligand DiI-acetylated low density lipoprotein to PBM, nor does another SR ligand, fucoidin, inhibit Abeta-PBM binding. Moreover, neither of three SR ligands alone stimulates neurotoxicity in PBM, nor antagonizes the ability of Abeta to activate PBM to a neurocytopathic state. Such findings suggest that Abeta's action is not dependent upon engagement of the SR ligand binding domain and raise doubts about the role of SR in Abeta neurotoxicity.
Antic
A
A
Blood-Brain Barrier Laboratory, Department of Pharmacology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut, 06030, USA.
Dzenko
K A
KA
Pachter
J S
JS
D000544:Alzheimer Disease / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D001665:Binding Sites / Q000502:physiology; D002232:Carbocyanines; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000378:metabolism / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D008565:Membrane Proteins; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D009474:Neurons / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D010446:Peptide Fragments / Q000633:toxicity*; D010587:Phagocytosis / Q000276:immunology; D011134:Polysaccharides / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011971:Receptors, Immunologic / Q000737:chemistry / Q000378:metabolism*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D015398:Signal Transduction / Q000502:physiology
D016229:Amyloid beta-Peptides; D000970:Antineoplastic Agents; C101089:CM-DiI; D002232:Carbocyanines; D008078:Cholesterol, LDL; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D010446:Peptide Fragments; D011134:Polysaccharides; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051122:Scavenger Receptors, Class B; C078592:amyloid beta-protein (1-40); C007789:fucoidan