[Identification and determination of Shenbaiye].
This study was amied at the method of identifying and determining Shenbaiye so that a ground can be provided for the quality requirements. Three Chinese materia medica (Radix Sophorae flavescentis, I; Cortex Phellodendri, II; Rhizoma Atractylodis, III) of Shenbaiye were identified by means of TLC successfully. The method in detail and the chromatogram were presented. The determination of I in Shenbiaye was carried out by solid phase cleaned-up and separated through neutral alumine column (10 x 1 cm i.d.) eluting with chloroform. This eluent was extracted with sulphuric acid (0.01 mol/L) and the excess of acids was titrated with sodium hydroxide (0.02 mol/L) using the methyl red as an indicator. The total alkaloid content of I was calculated as matrine of I in Shenbaiye. This method was established with a precision of 1.10% (n = 5) and the recoveries of 97.59 +/- 1.28% (n = 8). The results of determination of samples were reported.
Wu
C
C
Laboratory of Pharmaceutical Analysis, School of Pharmacy, Chengdu.
Zhou
Z
Z
He
Y
Y
D000470:Alkaloids / Q000032:analysis*; D002855:Chromatography, Thin Layer; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011807:Quinolizines; D000093842:Matrines
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D011807:Quinolizines; D000093842:Matrines