Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography.
Immunoaffinity column chromatography using an anti-ginsenoside Rb1 monoclonal antibody has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng). The combination of immunoaffinity column chromatography and an enzyme-linked immunosorbent assay (ELISA) was also investigated.
Fukuda
N
N
Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Tanaka
H
H
Shoyama
Y
Y
D000911:Antibodies, Monoclonal / Q000737:chemistry; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D036145:Ginsenosides; D007118:Immunoassay; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D012503:Saponins / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D036145:Ginsenosides; D010936:Plant Extracts; D012503:Saponins