IFN-alpha1 plasmid construct affords protection against HSV-1 infection in transfected L929 fibroblasts.
The purpose of the present study was to evaluate the resistance against herpes simplex virus type 1 (HSV-1) using an interferon-alpha1 (IFN-alpha1) transgene in specifically targeted cells in vitro. Transfection of mouse fibroblast L929 cells with an IFN-alpha1 plasmid construct reduced viral load and viral gene expression in a time-dependent fashion. Supernatants from IFN-alpha1-transfected cells augmented natural killer (NK) cell activity, and such an effect was antagonized with neutralizing antibody to IFN-alpha/beta. In addition, transfected cells displayed an increase in the IFN inducible genes (2',5'-oligoadenylate synthetase [2',5'-OAS], T cell-specific guanine nucleotide triphosphate-binding protein, IFN regulatory factor 1 [IRF-1], and major histocompatibility complex [MHC] class I) compared with plasmid vector-treated controls. Collectively, these results show that IFN-alpha1 transfection of cells in vitro induces or upregulates a spectrum of IFN-regulated genes involved in the direct or indirect antiviral action of this cytokine. In addition, the transgene significantly increases the resistance of transfected cells in vitro to HSV-1 infection.
Noisakran
S
S
Department of Microbiology, Immunology, and Parasitology, LSU Medical Center, New Orleans, LA 70112, USA.
Campbell
I L
IL
Carr
D J
DJ
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation; D015967:Gene Expression Regulation, Viral; D005805:Genes, MHC Class I; D005822:Genetic Vectors / Q000235:genetics*; D006183:H-2 Antigens / Q000096:biosynthesis; D050856:Interferon Regulatory Factor-1; D016898:Interferon-alpha / Q000235:genetics / Q000502:physiology*; D007739:L Cells / Q000187:drug effects / Q000821:virology*; D051379:Mice; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018139:Simplexvirus / Q000302:isolation & purification / Q000502:physiology*; D014162:Transfection; D014779:Virus Replication
D004268:DNA-Binding Proteins; D006183:H-2 Antigens; D050856:Interferon Regulatory Factor-1; D016898:Interferon-alpha; C494258:Irf1 protein, mouse; D010750:Phosphoproteins; D015088:2',5'-Oligoadenylate Synthetase; D019204:GTP-Binding Proteins