Interactions of bovine brain tubulin with pyridostigmine bromide and N,N'-diethyl-m-toluamide.
Pyridostigmine bromide (PB), an inhibitor of acetylcholinesterase, has been used as a prophylactic for nerve gas poisoning. N,N'-diethyl-m-toluamide (DEET) is the active ingredient in most insect repellents and is thought to interact synergistically with PB. Since PB can inhibit the binding of organophosphates to tubulin and since organophosphates inhibit microtubule assembly, we decided to examine the effects of PB and DEET on microtubule assembly as well as their interactions with tubulin, the subunit protein of microtubules. We found that PB binds to tubulin with an apparent Kd of about 60 microM. PB also inhibits microtubule assembly in vitro, although at higher concentrations PB induces formation of tubulin aggregates of high absorbance. Like PB, DEET is a weak inhibitor of microtubule assembly and also induces formation of tubulin aggregates. Many tubulin ligands stabilize the conformation of tubulin as measured by exposure of sulfhydryl groups and hydrophobic areas and stabilization of colchicine binding. PB appears to have very little effect on tubulin conformation, and DEET appears to have no effect. Neither compound interferes with colchicine binding to tubulin. Our results raise the possibility that PB and DEET may exert some of their effects in vivo by interfering with microtubule assembly or function, although high intracellular levels of these compounds would be required.
Prasad
V
V
Department of Biochemistry, University of Texas Health Science Center at San Antonio, 78284-7760, USA.
Scotch
R
R
Chaudhuri
A R
AR
Walss
C
C
Fathy
D B
DB
Miller
C
C
Ludueña
R F
RF
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D003078:Colchicine / Q000378:metabolism; D003671:DEET / Q000494:pharmacology*; D007302:Insect Repellents / Q000494:pharmacology*; D007700:Kinetics; D008870:Microtubules / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D011729:Pyridostigmine Bromide / Q000378:metabolism / Q000494:pharmacology*; D014316:Tritium; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism
D002800:Cholinesterase Inhibitors; D007302:Insect Repellents; D014404:Tubulin; D014316:Tritium; D003671:DEET; D011729:Pyridostigmine Bromide; D003078:Colchicine