Enhancement of growth and toxin production of Clostridium argentinense by co-culture with Pseudomonas mendocina.
The growth and toxin production of Clostridium argentinense in co-culture with Pseudomonas mendocina was examined in a micro-fermenter without aeration characterizing the association in terms of several growth parameters. The biomass obtained in co-cultures was 4.4 times higher than that in C. argentinense monocultures with total consumption of the carbon source. The pH and Eh attained in co-cultures at later stages of cultivation were suitable for toxin production by C. argentinense. In comparison with C. argentinense monocultures the production of toxin was 17.5 times higher with a specific toxicity of 0.56 LD50 per g of co-culture biomass.
Centorbi
H J
HJ
Facultad de Química Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, Argentina.
Silva
H J
HJ
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D003013:Clostridium / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051379:Mice; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism*
D001427:Bacterial Toxins; D003470:Culture Media; D005947:Glucose