title: [Evaluation of direct identification testing from positive blood culture bottles with MicroScan rapid identification panels].
abstract: We evaluated the direct identification method from growth-positive blood culture bottles using MicroScan Rapid ID panels (DADE BEHRING) for the purpose of rapid identification. The inoculum for Rapid ID panels were prepared using an isolation method from blood culture bottles by VACUTAINER (BD). McF 1.0 had a better result than McF 0.5 as the inoculum concentration for Rapid ID panels. Rapid ID panel identification results were effected by blood contamination for > or =0.3% of S. aureus and 0.9% of a strain of E. coli. Blood contamination from the bottle may cause an issue to the identification results. The accuracy of this direct identification testing was 72.0% (36 out of 50) for gram positives organisms and 88. 9% (80 out of 90) for gram negatives organisms. Although some strains including S. pyogenes, coagulase-negative staphylococci and non-Fermentative Gram Negative Rods had not identified correctly, this method provides a preliminary result within 3 hours and provides a fast turn around time. In conclusion, this method was considered as an effective method for routine testing.
authors.affiliation: Department of Clinical Laboratory, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan. cl-wada@hyo-med.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Yoshimoto
authors.forename: T
authors.initials: T
authors.lastname: Tozawa
mesh_terms: D001431:Bacteriological Techniques; D001769:Blood; D003470:Culture Media; D003955:Diagnostic Tests, Routine; D005069:Evaluation Studies as Topic; D006801:Humans
chemical_list: D003470:Culture Media