Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli.
A gas chromatographic analysis method was employed to determine the cellular fatty acid (CFA) profiles of spores and vegetative cells of some aerobic endospore-forming bacilli. The harvests of experimental strains were processed to obtain pure spores and acquire whole cell fatty acid methyl esters for the subsequent gas chromatographic analysis, and the corresponding vegetative cells were set as control. Evaluation of reproducibility of spore CFA components revealed that, provided under standardized experimental procedure, spore CFA composition was stable enough for research purposes. Fatty acids recovered in spores in greater quantities were saturated branched-chain acids containing 15 and 17 carbon atoms, similar to the vegetative cells. Commonly, the proportions of saturated branched-chain acids in spores were greater than in vegetative cells. The dendrograms obtained by cluster analysis provided some meaningful taxonomic information of the experimental strains. The fatty acids analysis of spores seems to be a promising supplementary tool for the chemotaxonomic research of aerobic endospore-forming bacilli.
Song
Y
Y
Laboratory for Analytical Microbiology, National Center of Biomedical Analysis, Institute of Microbiology and Epidemiology, Fengtai District, Beijing, PR China.
Yang
R
R
Guo
Z
Z
Zhang
M
M
Wang
X
X
Zhou
F
F
D001406:Bacillaceae / Q000737:chemistry* / Q000145:classification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D015203:Reproducibility of Results; D013171:Spores, Bacterial / Q000737:chemistry
D005227:Fatty Acids