Kinetic Footprinting of an RNA-Folding Pathway Using Peroxynitrous Acid.
Following footprints to discover a path is easier with peroxynitrous acid. The folding of the Tetrahymena ribozyme was studied using this readily available reagent to generate hydroxyl radicals for kinetic footprinting studies. The different domains of the ribozyme appear to assemble at different rates following an ordered, hierarchical pathway (see scheme).
Chaulk
SG
Department of Chemistry University of Toronto Toronto, ON, M5S-3H6 (Canada).
MacMillan
AM