title: [Analysis of prevalence of point mutations in codon 12 of oncogene K-ras from non-cancerous samples of cervical cytology positive for type 16 or 18 PVH].
abstract: Ninety-one non cancerous samples from genital specimens positives for VPH 16 or 18 and 27 non-infected samples as controls were studied. Mutations at codon 12 in K-ras gene was analyzed using enriched alelic PCR technique. Among the samples studied 17.58% showed mutations in this codon. Significant differences were observed between the control group (negative DNA-HPV) and positives DNA-HPV samples (p < 0.01). No differences were found between both viral types in relation to the mutation frequency. The presence of mutations in the K-ras gene in non cancerous cytological samples point out new questions about the role of mutations in proto-oncogenes and the development of cervical cancer.
authors.affiliation: Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina. cgolijow@fcv.medvet.unlp.edu.ar
authors.forename: C D
authors.initials: CD
authors.lastname: Golijow
authors.forename: S A
authors.initials: SA
authors.lastname: Mourón
authors.forename: M A
authors.initials: MA
authors.lastname: Gómez
authors.forename: F N
authors.initials: FN
authors.lastname: Dulout
mesh_terms: D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology*; D017354:Point Mutation; D015995:Prevalence; D014412:Tumor Virus Infections / Q000235:genetics* / Q000821:virology*; D014626:Vaginal Smears