title: Vaccination of seronegative volunteers with a human immunodeficiency virus type 1 env/rev DNA vaccine induces antigen-specific proliferation and lymphocyte production of beta-chemokines.
abstract: There is a pressing need to test novel vaccine concepts in an effort to develop an effective vaccine for human immunodeficiency virus (HIV) type 1. A phase I clinical study was done to test the immunogenicity of an HIV env/rev DNA vaccine, which was administered intramuscularly to HIV-1-seronegative persons. Subjects received 3 doses of vaccine at a single concentration (100 or 300 microgram) at 0, 4, 8, and 24 weeks. In at least 1 of multiple assays, the 6 subjects who received the 300-microgram dose had DNA vaccine-induced antigen-specific lymphocyte proliferative responses and antigen-specific production of both interferon-gamma and beta-chemokine. Furthermore, 4 of 5 subjects in the 300 microgram-dose group responded to both the rev and env components of the vaccine. The responses did not persist within inoculated individuals and scored in different individuals at different times in the trial. This study supports that HIV-1 DNA vaccine antigens can stimulate multiple immune responses in vaccine-naive individuals, and it warrants additional studies designed to enhance DNA vaccine immunogenicity.
authors.affiliation: Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Boyer
authors.forename: A D
authors.initials: AD
authors.lastname: Cohen
authors.forename: S
authors.initials: S
authors.lastname: Vogt
authors.forename: K
authors.initials: K
authors.lastname: Schumann
authors.forename: B
authors.initials: B
authors.lastname: Nath
authors.forename: L
authors.initials: L
authors.lastname: Ahn
authors.forename: K
authors.initials: K
authors.lastname: Lacy
authors.forename: M L
authors.initials: ML
authors.lastname: Bagarazzi
authors.forename: T J
authors.initials: TJ
authors.lastname: Higgins
authors.forename: Y
authors.initials: Y
authors.lastname: Baine
authors.forename: R B
authors.initials: RB
authors.lastname: Ciccarelli
authors.forename: R S
authors.initials: RS
authors.lastname: Ginsberg
authors.forename: R R
authors.initials: RR
authors.lastname: MacGregor
authors.forename: D B
authors.initials: DB
authors.lastname: Weiner
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D019742:Chemokines, CC / Q000096:biosynthesis*; D015752:Genes, env; D015757:Genes, rev; D018023:HIV Seronegativity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D019742:Chemokines, CC; D019444:Vaccines, DNA