Evaluation of heparin immobilized chitosan-PEG microbeads for charcoal encapsulation and endotoxin removal.
A technique is described to encapsulate activated charcoal for hemoperfusion to be used in an artificial liver support. Activated charcoal was encapsulated within chitosan-PEG matrix and subsequently surface modified with PGE1 or heparin (hep-AC-PEGCB) via the glutaraldehyde functionalities. This novel matrix was used as the supports for perfusion of endotoxin, under a flow rate of 30 ml/mt. Endotoxin adsorption was quantitatively measured by the method of Limulus Amebocyte lysate test. It seems, the hep-AC-PEGCB may be a good adsorbent system for the removal of toxic endotoxin, and the system may be useful for detoxification of blood. The hep-AC-PEGCB matrix had improved biocompatibility as demonstrated from their hemolytic potential and charcoal release. However, further studies are needed to determine their behaviour under clinical conditions.
Chandy
T
T
Biomedical Engineering Institute, University of Minnesota, Minneapolis 55455, USA.
Rao
G H
GH
D000527:Alprostadil / Q000378:metabolism; D001672:Biocompatible Materials / Q000378:metabolism / Q000493:pharmacokinetics; D002606:Charcoal / Q000378:metabolism / Q000493:pharmacokinetics*; D002686:Chitin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics; D048271:Chitosan; D004339:Drug Compounding / Q000379:methods* / Q000592:standards; D004731:Endotoxins / Q000493:pharmacokinetics*; D005069:Evaluation Studies as Topic; D005343:Fibrinolytic Agents; D006464:Hemoperfusion / Q000379:methods*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D011092:Polyethylene Glycols / Q000378:metabolism / Q000493:pharmacokinetics*; D013927:Thrombosis / Q000517:prevention & control
D001672:Biocompatible Materials; D004731:Endotoxins; D005343:Fibrinolytic Agents; D002686:Chitin; D002606:Charcoal; D011092:Polyethylene Glycols; D006493:Heparin; D048271:Chitosan; D000527:Alprostadil