Application of short tandem repeat of genomic DNA and mitochondrial DNA for identification of mixed-up tissue specimens.
Deoxyribonucleic acid (DNA) typing methods, short tandem repeat of genomic DNA and mitochondrial DNA with the use of polymerase chain reaction amplification, were applied to formalin-fixed, paraffin-embedded tissues submitted for diagnosis, to identify and sort out mixed-up tissue specimens. These techniques were found to be reliable, reproducible and specific for personal identification, and thus to eliminate the need for further examinations and to prevent unnecessary surgery.
Sano
K
K
Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan. kenjisa@sch.md.shinshu-u.ac.jp
Takayanagi
K
K
Kaneko
T
T
Liu
C
C
Kinoshita
T
T
Nakamura
N
N
Asamura
H
H
Ito
M
M
Fukushima
H
H
Hotchi
M
M
D000368:Aged; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D016678:Genome; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D020080:Tandem Repeat Sequences / Q000235:genetics*
D017931:DNA Primers; D004272:DNA, Mitochondrial; D004273:DNA, Neoplasm