Dolichin, a new chitinase-like antifungal protein isolated from field beans (Dolichos lablab).
An antifungal protein, possessing a molecular weight of 28 kDa and an N-terminal sequence resembling chitinases, has been purified from the seeds of the field bean Dolichos lablab. The procedure involved extraction with aqueous buffer, affinity chromatography on Affi-gel blue gel, and ion exchange chromatography on CM-Sepharose. The protein, designated dolichin, exhibited antifungal activity against the fungi Fusarium oxysporum, Rhizoctonia solani, and Coprinus comatus. Dolichin was capable of inhibiting human immunodeficiency virus (HIV) reverse transcriptase and alpha- and beta-glucosidases which are glycohydrolases implicated in HIV infection. It had very low ribonuclease and cell-free translation-inhibitory activities.
Ye
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Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Wang
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HX
Ng
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TB
D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002688:Chitinases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007887:Fabaceae / Q000737:chemistry* / Q000235:genetics; D005658:Fungi / Q000187:drug effects; D005959:Glucosidases / Q000037:antagonists & inhibitors; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D015497:HIV-1 / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
D019380:Anti-HIV Agents; D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D010940:Plant Proteins; D054303:HIV Reverse Transcriptase; D005959:Glucosidases; D002688:Chitinases