A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody.
OBJECTIVE
To develop and test a new latex slide agglutination test (MPO-LSAT) to detect antimyeloperoxidase (anti-MPO) antibody in serum.


METHODS
Latex bead coating was adjusted to give maximum sensitivity by attending to latex size, MPO to latex ratio for coupling, ratio of diluted serum to MPO-latex, reaction time and temperature for coupling, and reaction time for agglutination. Inhibition studies were performed using MPO, proteinase 3, bactericidal/permeability increasing protein, and lactoferrin.


RESULTS
There was very good correlation between this test and the conventional anti-MPO enzyme linked immunosorbent assay (ELISA): 81% of sera positive in the ELISA were positive by MPO-LSAT. MPO-LSAT results correlated better with IgM anti-MPO than with IgG anti-MPO.


CONCLUSIONS
MPO-LSAT is a simple diagnostic test that is potentially useful in the clinical laboratory as a rapid screening tool for vasculitic diseases.
Ko
K H
KH
Department of Pathology, University of Hong Kong, Hong Kong.
Lee
S S
SS
Lawton
J W
JW
D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods; D009195:Peroxidase / Q000276:immunology*; D012680:Sensitivity and Specificity; D014657:Vasculitis / Q000175:diagnosis*
D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies; D009195:Peroxidase