Photo-cross-linkable oligonucleotide probes for in situ hybridization assays.
In situ hybridization techniques have been an important research tool since first introduced 30 years ago, and more recently clinical applications have been expanding greatly. Still, further improvements in the assay sensitivity and protocols that are amenable to routine clinical use are desired. We use a novel photo-cross-linking technology to irreversibly bind short oligonucleotide probes to the target sequence following a hybridization period. The cross-linking agent is incorporated into the backbone of the probe and is activated to react with pyrimidines in the opposite strand by near-UV (300-370 nm) irradiation. By locking the probe to the target, very stringent wash conditions can be used that would otherwise completely remove probes that are hybridized but not cross-linked to the target. Consequently, the probe-specific signal is maximized, while the background signal is minimized to the greatest extent possible with the stringency of the wash. The use of short, photo-cross-linkable probes presents a new strategy for maximizing the sensitivity of probe hybridization or signal amplification-based in situ techniques.
Huan
B
B
NAXCOR, Menlo Park, CA 94025, USA.
Van Atta
R
R
Cheng
P
P
Wood
M L
ML
Zychlinsky
E
E
Albagli
D
D
D001483:Base Sequence; D001709:Biotechnology; D002460:Cell Line; D002584:Cervix Uteri / Q000821:virology; D003432:Cross-Linking Reagents / Q000737:chemistry; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D010777:Photochemistry
D003432:Cross-Linking Reagents; D004279:DNA, Viral; D015345:Oligonucleotide Probes