Comparative studies on detecting CAg in urine of acute schistosomiasis patients by mAb-RIHA and mAb-DotELISA.
Urine was concentrated 20-fold for assay for CAg of Schistosoma japonicum. mAb-RIHA and mAb-DotELISA were positive in 78, 31% and 65.06% of cases respectively, of 83 patients with acute schistosomiasis. The false positive rates in 101 healthy controls were 14.85% and 0%, respectively. Cross-reactions (using mAb-RIHA) were seen in 16.36% and 14.28% of patients with clonorchiasis, 49 patients with ankylostomiasis, respectively. Corresponding figures for mAb-DotELISA were 0% and 0%.
Liu
X M
XM
Anhui Provincial Institute of Parasitic Diseases, Wuhu, People's Republic of China.
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D016022:Case-Control Studies; D003003:Clonorchiasis / Q000276:immunology / Q000652:urine; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D006023:Glycoproteins / Q000276:immunology* / Q000652:urine*; D015801:Helminth Proteins / Q000276:immunology* / Q000652:urine*; D006725:Hookworm Infections / Q000276:immunology / Q000652:urine; D006801:Humans; D015203:Reproducibility of Results; D012554:Schistosomiasis japonica / Q000188:drug therapy / Q000276:immunology* / Q000652:urine*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D006023:Glycoproteins; D015801:Helminth Proteins; C090907:circulating anodic antigen, Schistosoma mansoni