title: A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody.
abstract: OBJECTIVE
  To develop and test a new latex slide agglutination test (MPO-LSAT) to detect antimyeloperoxidase (anti-MPO) antibody in serum.
  
  
  METHODS
  Latex bead coating was adjusted to give maximum sensitivity by attending to latex size, MPO to latex ratio for coupling, ratio of diluted serum to MPO-latex, reaction time and temperature for coupling, and reaction time for agglutination. Inhibition studies were performed using MPO, proteinase 3, bactericidal/permeability increasing protein, and lactoferrin.
  
  
  RESULTS
  There was very good correlation between this test and the conventional anti-MPO enzyme linked immunosorbent assay (ELISA): 81% of sera positive in the ELISA were positive by MPO-LSAT. MPO-LSAT results correlated better with IgM anti-MPO than with IgG anti-MPO.
  
  
  CONCLUSIONS
  MPO-LSAT is a simple diagnostic test that is potentially useful in the clinical laboratory as a rapid screening tool for vasculitic diseases.
authors.affiliation: Department of Pathology, University of Hong Kong, Hong Kong.
authors.forename: K H
authors.initials: KH
authors.lastname: Ko
authors.forename: S S
authors.initials: SS
authors.lastname: Lee
authors.forename: J W
authors.initials: JW
authors.lastname: Lawton
mesh_terms: D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007841:Latex Fixation Tests / Q000379:methods; D009195:Peroxidase / Q000276:immunology*; D012680:Sensitivity and Specificity; D014657:Vasculitis / Q000175:diagnosis*
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies; D009195:Peroxidase