Heat shock protein (hsp 70)-related epitopes are common allergenic determinants for barley and corn antigens.
IgE reactive components of barley and corn were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with sera from workers exposed to complex bioaerosols. The antibody made against Arabdopsis heat shock protein (hsp 70) was used to identify those components equivalent to hsp 70 in molecular size. Components with a molecular mass of 69 kDa and 33 kDa were positively reacted, and immunoblots of two-dimensional polyacrylamide gel electrophoresis were compared.
Chiung
Y M
YM
Institute of Occupational Safety and Health, Academia Sinica, Taipei, Taiwan. chiun@mail.cla.gov.tw
Lin
B L
BL
Yeh
C H
CH
Lin
C Y
CY
D000485:Allergens / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D000939:Epitopes / Q000032:analysis*; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D001467:Hordeum / Q000276:immunology*; D006801:Humans; D003313:Zea mays / Q000276:immunology*
D000485:Allergens; D000939:Epitopes; D018840:HSP70 Heat-Shock Proteins