title: Occludin modulates transepithelial migration of neutrophils.
abstract: Neutrophils cross epithelial sheets to reach inflamed mucosal surfaces by migrating along the paracellular route. To avoid breakdown of the epithelial barrier, this process requires coordinated opening and closing of tight junctions, the most apical intercellular junctions in epithelia. To determine the function of epithelial tight junction proteins in this process, we analyzed neutrophil migration across monolayers formed by stably transfected epithelial cells expressing wild-type and mutant occludin, a membrane protein of tight junctions with four transmembrane domains and both termini in the cytosol. We found that expression of mutants with a modified N-terminal cytoplasmic domain up-regulated migration, whereas deletion of the C-terminal cytoplasmic domain did not have an effect. The N-terminal cytosolic domain was also found to be important for the linear arrangement of occludin within tight junctions but not for the permeability barrier. Moreover, expression of mutant occludin bearing a mutation in one of the two extracellular domains inhibited neutrophil migration. The effects of transfected occludin mutants on neutrophil migration did not correlate with their effects on selective paracellular permeability and transepithelial electrical resistance. Hence, specific domains and functional properties of occludin modulate transepithelial migration of neutrophils.
authors.affiliation: Département de Biologie Cellulaire, Université de Genève, Sciences III, 30 Quai Ernest-Ansermet, 1211 Genève-4, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Huber
authors.forename: M S
authors.initials: MS
authors.lastname: Balda
authors.forename: K
authors.initials: K
authors.lastname: Matter
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D002465:Cell Movement / Q000502:physiology; D004285:Dogs; D017097:Electric Impedance; D004847:Epithelial Cells / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008854:Microscopy, Electron; D009504:Neutrophils / Q000502:physiology* / Q000648:ultrastructure; D062793:Occludin; D010750:Phosphoproteins / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D019108:Tight Junctions; D014162:Transfection; D062826:Zonula Occludens-1 Protein
chemical_list: D000199:Actins; D008565:Membrane Proteins; D062793:Occludin; D010750:Phosphoproteins; D062826:Zonula Occludens-1 Protein