Expression of interleukin-6 in human dorsal root ganglion cells.
The expression of Interleukin-6 (IL-6) was studied in normal dorsal root ganglia (DRG) of juvenile and foetal humans, using immunohistochemistry and in situ hybridization techniques. There was an expression of IL-6-like immunoreactivity in more than 75% out of neuronal cells in the juvenile ganglia with a peripheral localization, and also an expression in the foetal ganglion cells. There was a co-localization of IL-6 with substance P (SP) and calcitonin gene-related peptide (CGRP) in more than 60% of the DRG cells, respectively. By in situ hybridization 0.9% of the cells in the juvenile ganglia and 1.1% of the cells in the foetal ganglia showed a positive signal for IL-6. In addition, expression of IL-6 was found in juvenile medulla spinalis, preferentially in the white matter.
Nordlind
K
K
Dermatology and Venereology, Department of Medical Sciences, University Hospital, S-751 85, Uppsala, Sweden. klas.nordlind@hud.uu.se
Eriksson
L
L
Seiger
A
A
Bakhiet
M
M
D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002648:Child; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015850:Interleukin-6 / Q000032:analysis* / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013373:Substance P / Q000032:analysis
D015850:Interleukin-6; D012333:RNA, Messenger; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide