title: Angiogenesis inhibitor, TNP-470, suppresses growth of peritoneal disseminating foci.
abstract: OBJECTIVE
  Angiogenesis is critical not only for growth of primary tumors but also for cells established at distant organs. We investigated the effects of angiogenesis inhibitor, TNP-470, on the establishment and growth of intraperitoneally inoculated human gastric cancer cell line, MKN-45, and survival of nude mice with this tumor.
  
  
  METHODS
  Human gastric cancer cell line, MKN-45, were injected into the peritoneal cavity of an ICR nude mouse and a model of peritoneal dissemination was developed. TNP-470 was injected subcutaneously every other day from day 1 until sacrifice or death. The effects of TNP-470 on MKN-45 cells were also examined in vitro.
  
  
  RESULTS
  Although the number of disseminated foci was not significantly different, the maximum size was significantly smaller in a TNP-treated group than those of a control. Survival time was significantly longer in a TNP-treated group. TNP-470 demonstrated no growth inhibition of MKN45 cells in vitro.
  
  
  CONCLUSIONS
  Those results suggested that anti-angiogenic agent, TNP-470, might be effective in treating peritoneal dissemination of gastric cancer by inhibiting growth of the seeded tumor cells on the peritoneum.
authors.affiliation: Third Department of Surgery, Kanagawa Cancer Center, Yokohama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: S
authors.initials: S
authors.lastname: Yanoma
authors.forename: A
authors.initials: A
authors.lastname: Tsuburaya
authors.forename: O
authors.initials: O
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Sairenji
authors.forename: H
authors.initials: H
authors.lastname: Motohashi
authors.forename: Y
authors.initials: Y
authors.lastname: Noguchi
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects; D003510:Cyclohexanes; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D000077609:O-(Chloroacetylcarbamoyl)fumagillol; D010534:Peritoneal Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D012717:Sesquiterpenes / Q000627:therapeutic use*; D018709:Statistics, Nonparametric; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D000903:Antibiotics, Antineoplastic; D003510:Cyclohexanes; D012717:Sesquiterpenes; D000077609:O-(Chloroacetylcarbamoyl)fumagillol