title: Mannanase Man26A from Cellulomonas fimi has a mannan-binding module.
abstract: A modular mannanase (Man26A) from the bacterium Cellulomonas fimi contains a mannan-binding module (Man26Abm) that binds to soluble but not to insoluble mannans. Man26Abm does not bind to cellulose, chitin or xylan. The K(d) for binding of Man26Abm to locust bean gum (LBG) is approximately 0.2 microM. Man26A is the first mannanase reported to contain a mannan-binding module.
authors.affiliation: Department of Microbiology and Immunology, University of British Columbia, Vancouver, B.C., Canada.
authors.forename: D
authors.initials: D
authors.lastname: Stoll
authors.forename: A
authors.initials: A
authors.lastname: Boraston
authors.forename: H
authors.initials: H
authors.lastname: Stålbrand
authors.forename: B W
authors.initials: BW
authors.lastname: McLean
authors.forename: D G
authors.initials: DG
authors.lastname: Kilburn
authors.forename: R A
authors.initials: RA
authors.lastname: Warren
mesh_terms: D000595:Amino Acid Sequence; D001420:Bacteria, Aerobic / Q000201:enzymology*; D001483:Base Sequence; D001665:Binding Sites; D008351:Mannans / Q000378:metabolism*; D008361:Mannosidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D044902:beta-Mannosidase
chemical_list: D008351:Mannans; D008361:Mannosidases; D044902:beta-Mannosidase