Epicubenol synthase. Origin of the oxygen atom of a bacterial sesquiterpene alcohol.
Incubation of epicubenol synthase with farnesyl pyrophosphate in the presence of 11.1 atom% H2(18)O gave epicubenol (2) in which the hydroxyl oxygen atom was shown to be derived exclusively from water, as established by GC-selected ion monitoring MS of the derived TMS-epicubenol derivative (15).
Cane
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Department of Chemistry, Brown University, Providence, RI, 02912-9108, USA. david_cane@brown.edu
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D000438:Alcohols / Q000737:chemistry*; D019753:Intramolecular Lyases / Q000737:chemistry*; D007535:Isomerases / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010103:Oxygen Isotopes; D012717:Sesquiterpenes / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology
D000438:Alcohols; D010103:Oxygen Isotopes; D012717:Sesquiterpenes; D007535:Isomerases; C404719:epicubenol synthase; D019753:Intramolecular Lyases