title: Crystallization and preliminary X-ray diffraction studies of cytochrome c6 from Porphyra yezoensis.
abstract: Cytochrome c(6) from the red alga Porphyra yezoensis has been purified and crystallized by the sitting-drop vapour-diffusion method. Two different crystal forms, tetragonal and orthorhombic, were obtained. The tetragonal crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell dimensions a = 49.33 (2), c = 83.70 (10) A. The orthorhombic crystals belong to space group P2(1)2(1)2(1), with unit-cell dimensions a = 46.74 (2), b = 49.42 (1), c = 37.11 (1) A. Absorption spectra of the crystals showed that the tetragonal form was oxidized and the orthorhombic form was reduced.
authors.affiliation: Department of Biomolecular Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Nakahara
authors.forename: A
authors.initials: A
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Yamasaki
authors.forename: S
authors.initials: S
authors.lastname: Katoh
authors.forename: S
authors.initials: S
authors.lastname: Ohshima
authors.forename: I
authors.initials: I
authors.lastname: Oonishi
authors.forename: A
authors.initials: A
authors.lastname: Uchida
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D003580:Cytochromes / Q000737:chemistry* / Q000302:isolation & purification*; D045348:Cytochromes f; D010084:Oxidation-Reduction; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D003580:Cytochromes; D045348:Cytochromes f