Mode of action of sesame lignans in protecting low-density lipoprotein against oxidative damage in vitro.
We investigated the antioxidant properties of sesaminol, a major component of sesame oil, on the oxidative modification of human low-density lipoprotein (LDL) in vitro. Sesaminol inhibited the Cu2+-induced lipid peroxidation in LDL in a concentration-dependent manner with an IC50 36.0 +/- 10.0 nM. Sesaminol was a more effective scavenger than either alpha-tocopherol or probucol in reducing the peroxyl radicals derived from 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) in aqueous solution. In addition, as determined by the secondary products of lipid peroxidation identified by using immunochemical methods, sesaminol completely inhibited the formation of 4-hydroxy-nonenal (4-HNE)- and malondialdehyde (MDA)-adducts in a concentration-dependent manner. Probucol and alpha-tocopherol at the same concentration exhibited a lesser inhibitory effect. Our findings suggest that sesaminol is a potentially effective antioxidant that can protect LDL against the oxidation.
Kang
M H
MH
Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Japan.
Naito
M
M
Sakai
K
K
Uchida
K
K
Osawa
T
T
D000975:Antioxidants / Q000494:pharmacology*; D004149:Dioxoles / Q000494:pharmacology*; D005260:Female; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D017705:Lignans / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000187:drug effects / Q000378:metabolism*; D012715:Sesame Oil / Q000737:chemistry / Q000494:pharmacology
D000975:Antioxidants; D004149:Dioxoles; D005663:Furans; D017705:Lignans; D008077:Lipoproteins, LDL; C066016:sesaminol; D012715:Sesame Oil