Trypanosoma cruzi: molecular cloning of a gene coding for a putative vacuolar protein.
We describe the characterization of Tc38, a Trypanosoma cruzi gene coding for a 337-amino-acid protein with a predicted molecular mass of 38 kDa. Tc38 presents similarities to the plant storage vacuolar protein gamma-3-hordein involved in the transport and targeting of prolamins to the vacuole of developing barley endosperm. Western blot analysis using a polyclonal antiserum against recombinant Tc38 revealed that the protein is differentially expressed in the different life stages of the parasite, showing a higher expression in the epimastigote and tripomastigote stages. Immunofluorescence studies suggest that the protein is located in putative vacuolar structures in epimastigotes. The functionality of this protein in T. cruzi remains to be elucidated.
Robello
C
C
Departmento de Bioquímica, Facultad de Ciencias, Universidad de la República, Montevideo-Uruguay.
Dallagiovanna
B
B
Castanys
S
S
Gamarro
F
F
Ehrlich
R
R
D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D017125:Genes, Protozoan; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D016053:RNA, Protozoan / Q000032:analysis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000235:genetics*; D014617:Vacuoles / Q000737:chemistry
D015800:Protozoan Proteins; D016053:RNA, Protozoan; D011994:Recombinant Proteins