Effects of recombinant human FSH (rhFSH), urinary purified FSH (uFSH), and hMG on small preantral follicles and tertiary follicles from normal adult and androgen-sterilized female mice.
OBJECTIVE
To examine the stage-specific follicular response to recombinant human FSH (rhFSH), urinary FSH (uFSH), and hMG preparations.


METHODS
In vitro follicle culture.


METHODS
Small preantral and tertiary follicles isolated from adult normal BDF-1 mice and androgen-sterilized mice were cultured with rhFSH, uFSH, and hMG for 4 days.


METHODS
Follicular diameter. Immunoreactive inhibin, E2, and progesterone concentrations in cultured medium.


RESULTS
The minimal effective dose of rhFSH, uFSH, and hMG for the follicular growth of small preantral follicles from normal mice was 10 mIU/mL, 1 mIU/mL, and 0.1 mIU/mL, respectively. For tertiary follicles from normal mice, the minimal effective dose of rhFSH, uFSH, and hMG was 10 mIU/mL, 10 mIU/mL, and 1 mIU/mL, respectively. The minimal effective dose of hMG for the follicular growth of small preantral follicles from androgen-sterilized mice was 0.01 mIU/mL, and that of rhFSH and uFSH on tertiary follicles from androgen-sterilized mice was 1 mIU/mL and 10 mIU/mL, respectively. No significant increase was found in the follicular diameter of the tertiary follicles from androgen-sterilized mice as a result of stimulation by hMG, but an hMG dose of >10 mIU/mL produced a significant increase in progesterone secretion.


CONCLUSIONS
Human menopausal gonadotropin preparation acts detrimentally on follicles from androgen-sterilized mice by increasing the sensitivity of small preantral follicles to FSH and by inducing the luteinization of tertiary follicles.
Liu
X
X
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Maebashi, Japan.
Andoh
K
K
Mizunuma
H
H
Kamijo
T
T
Kikuchi
N
N
Yamada
K
K
Ibuki
Y
Y
D000367:Age Factors; D000728:Androgens / Q000494:pharmacology; D000818:Animals; D000858:Anovulation / Q000139:chemically induced / Q000503:physiopathology*; D002478:Cells, Cultured; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification / Q000494:pharmacology* / Q000652:urine*; D006801:Humans; D007265:Inhibins / Q000378:metabolism; D008596:Menotropins / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology*; D011085:Polycystic Ovary Syndrome / Q000503:physiopathology; D011374:Progesterone / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
D000728:Androgens; D011994:Recombinant Proteins; D011374:Progesterone; D004958:Estradiol; D007265:Inhibins; D008596:Menotropins; D005640:Follicle Stimulating Hormone