[Influences of sperm quality and quantity on fertilization, cleavage rates and quality of embryos in in-vitro fertilization].
OBJECTIVE
To examine the influences of the quality and quantity of seminal sperm and inseminational sperm on in-vitro fertilization (IVF) outcomes.


METHODS
chi-square tests were used to analyze the impacts of both semen and inseminated sperm concentrations and percentages of different grade motile sperm on the fertilization rates in 481 matured and 273 immatured oocytes and cleavage rate percentage of embryo with low quality in embryos obtained from 92 IVF-embryo transfer cycles.


RESULTS
The fertilization rates of both kinds of oocytes and cleavage rate of fertilizaed matured oocyte significantly decreased (P < 0.05) as the concentrations and percentages of grade a and b sperms in semen reduced. The rates of embryos with abnormal morphology were significantly dropped when the above sperm parameters increased (P < 0.05). Meanwhile, it was also found that the concentration and motility of inseminated sperm could also affect the above three IVF parameters significantly (P < 0.05).


CONCLUSIONS
Highly active semen and inseminated sperm play important roles not only in fertilization, but also in cleavage and embryo development during IVF procedure.
Jin
F
F
Affiliated Obstetrics and Gynecology Hospital, Zhejiang Medical University, Hangzhou.
Shi
Y
Y
Zhou
F
F
D002970:Cleavage Stage, Ovum; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D009865:Oocytes; D013081:Sperm Motility