Simple and reliable high-performance liquid chromatography fluorimetric procedure for the determination of amphetamine-derived designer drugs.
The paper describes a HPLC-fluorimetric procedure for the determination of methylenedioxyamphetamine, methylenedioxymethamphetamine, methylenedioxyethamphetamine and N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine in urine, serum, saliva and street samples, that features interesting advantages over other procedures previously described. The method requires a very small sample volume (100 microl) and no extraction, lacks matrix effect, and is not time consuming. Linearity was in the range 50-1000 ng/ml regardless of matrix. Sensitivity and detection limit were 50 ng/ml and 10 ng/ml, respectively, but they may reach 10 ng/ml and 2 ng/ml if a slight modification is introduced in the procedure. Intra- and inter-day precision were always within 5% and 8%, respectively. Recovery was satisfactory for all matrices. The described procedure could be successfully used for clinical, epidemiological and forensic applications.
Mancinelli
R
R
Clinical Biochemistry Department, Istituto Superiore di Sanità, Rome, Italy. t.macchia@iss.it
Gentili
S
S
Guiducci
M S
MS
Macchia
T
T
D000662:Amphetamines / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015198:Designer Drugs / Q000032:analysis*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000662:Amphetamines; D015198:Designer Drugs