CDK inactivation is the only essential function of the APC/C and the mitotic exit network proteins for origin resetting during mitosis.
Passage through mitosis is required to reset replication origins for the subsequent S phase. During mitosis, a series of biochemical reactions involving cyclin-dependent kinases (CDKs), the anaphase promoting complex or cyclosome (APC/C), and a mitotic exit network including Cdc5, 14, and 15 coordinates the proper separation and segregation of sister chromatids. Here we show that cyclin B/CDK inactivation can drive origin resetting in either early S phase or mitosis. This origin resetting occurs efficiently in the absence of APC/C function and mitotic exit network function. We conclude that CDK inactivation is the single essential event in mitosis required to allow pre-RC assembly for the next cell cycle.
Noton
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ICRF Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom.
Diffley
J F
JF
D064173:Anaphase-Promoting Complex-Cyclosome; D017489:CDC28 Protein Kinase, S cerevisiae / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002842:Chromatids / Q000502:physiology; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D005838:Genotype; D008025:Ligases / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D017027:Protein Tyrosine Phosphatases; D016601:RNA-Binding Proteins; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases
C074860:CDC14 protein, S cerevisiae; C082279:CDC15 protein; C513239:CEF1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D017489:CDC28 Protein Kinase, S cerevisiae; D018844:Cyclin-Dependent Kinases; D017027:Protein Tyrosine Phosphatases; D019204:GTP-Binding Proteins; D008025:Ligases