Synthesis and characterization of aromatic ring-based cationic lipids for gene delivery in vitro and in vivo.
A new series of cationic lipids has been synthesized for gene delivery using 3,5-dihydroxybenzyl alcohol as the backbone and starting material. Using CMV driven expression system and luciferase gene as a reporter, we demonstrated that the transfection activity of these new lipids when formulated with Tween 80 as co-lipid is comparable to that of DOTAP, one of the most commonly used cationic lipids for transfection. Among the four different cell lines tested including murine melanoma BL-6 cells, human embryonic kidney 293 cells, HepG2 and HeLa cells, the highest transgene expression was seen in 293 cells. Results from in vivo experiments using mice as an animal model show that these cationic lipids preferentially transfect the cells in the lung upon tail vein administration. The cationic lipid, N,N,N-trimethyl-N-[3,5-bis(tetradecyloxy)benzyl] ammonium bromide 4c(di-C14:0) with two 14-hydrocarbon chains exhibits the best transfection activity. These results suggest that these new aromatic ring-based cationic lipids are useful transfection reagents for both in vitro and in vivo gene transfer studies.
Ren
T
T
Department of Pharmaceutical Science, School of Pharmacy, University of Pittsburgh, PA 15261, USA.
Zhang
G
G
Song
Y K
YK
Liu
D
D
D000818:Animals; D019905:Benzyl Alcohol / Q000737:chemistry*; D002412:Cations / Q000138:chemical synthesis / Q000737:chemistry; D002460:Cell Line; D016503:Drug Delivery Systems / Q000379:methods*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D018014:Gene Transfer Techniques; D006801:Humans; D066298:In Vitro Techniques; D008055:Lipids / Q000138:chemical synthesis / Q000737:chemistry; D008081:Liposomes; D008156:Luciferases / Q000235:genetics; D008168:Lung / Q000378:metabolism; D008545:Melanoma / Q000235:genetics; D051379:Mice; D011136:Polysorbates / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured
D002412:Cations; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; D008081:Liposomes; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds; D008156:Luciferases; D019905:Benzyl Alcohol; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane