CD11b/CD18-coated microspheres attach to E-selectin under flow.
Neutrophils can attach to E-selectin under flow. Proposed ligands for E-selectin carry SLe(x)-type glycans. The leukocyte beta2 integrins are glycosylated with SLe(x). Thus, we speculated that beta2 integrins could support attachment to E-selectin. To test this hypothesis, we coated 10-microm-diameter microspheres with purified CD11b/CD18 (alphaMbeta2) and investigated the adhesion of the resulting alphaMbeta2 microspheres to E-selectin. Under in vitro flow conditions, the alphaMbeta2 microspheres attached to Chinese hamster ovary cells expressing E-selectin (CHO-E) and 4-h interleukin-1beta-activated human umbilical vein endothelial cells (HUVEC). At a shear stress of 1.8 dynes/cm2, the attachment events were eliminated by pretreatment of the cellular monolayers with a mAb to E-selectin. alphaMbeta2 microspheres did not attach to untransfected CHO cells or unactivated HUVEC at 1.8 dynes/cm2. Taken together, the results strongly suggest that the CD11b/CD18-E-selectin bond has sufficient biophysical properties to mediate attachment of neutrophil-sized particles to E-selectin under flow.
Crutchfield
K L
KL
Department of Biomedical Engineering, The University of Memphis, Tennessee, USA.
Shinde Patil
V R
VR
Campbell
C J
CJ
Parkos
C A
CA
Allport
J R
JR
Goetz
D J
DJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D018821:CD18 Antigens / Q000276:immunology / Q000378:metabolism*; D016466:CHO Cells / Q000166:cytology / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D019040:E-Selectin / Q000235:genetics / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D016177:Macrophage-1 Antigen / Q000276:immunology / Q000378:metabolism*; D008863:Microspheres; D009439:Neuraminidase / Q000494:pharmacology; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012212:Rheology; D000080506:Sialyl Lewis X Antigen; D014162:Transfection; D014471:Umbilical Veins
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D019040:E-Selectin; D005732:Gangliosides; D016177:Macrophage-1 Antigen; D011993:Recombinant Fusion Proteins; D000080506:Sialyl Lewis X Antigen; C094688:sialyl Lewis(x) ganglioside; D005340:Fibrinogen; D009439:Neuraminidase