Suppression of GD1alpha ganglioside-mediated tumor metastasis by liposomalized WHW-peptide.
GD1alpha ganglioside-replica peptides were recently isolated from a phage-displayed random pentadecapeptide library by assaying for inhibition of adhesion of RAW117-H10 lymphosarcoma cells to hepatic sinusoidal microvessel endothelial (HSE) cells. We show here that the Trp-His-Trp (WHW) peptide was identified as a minimal sequence of the GD1alpha-replica peptide WHWRHRIPLQLAAGR. The addition of WHW peptide-attached liposomes displayed efficient inhibition of liver metastasis of RAW117-H10 cells as well as of GD1alpha-mediated adhesion of RAW117-H10 cells to HSE cells in vitro. These results suggest that engineered liposomes for peptide delivery are applicable to treatment for metastasis.
Takikawa
M
M
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Kikkawa
H
H
Asai
T
T
Yamaguchi
N
N
Ishikawa
D
D
Tanaka
M
M
Ogino
K
K
Taki
T
T
Oku
N
N
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D004337:Drug Carriers; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors; D008081:Liposomes; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D010455:Peptides / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004337:Drug Carriers; C404945:GD1alpha-replica peptide; D008081:Liposomes; D010455:Peptides; D011506:Proteins; C064020:ganglioside GD1alpha; D005677:G(M1) Ganglioside