title: Multiple intrasplenic hepatocyte transplantations in the dalmatian dog.
abstract: BACKGROUND
  Hepatocyte transplantation is an attractive potential treatment for liver-based inborn errors of metabolism and for fulminant hepatic failure. Dalmatian dogs have a metabolic error that results in hyperuricosuria. This report focuses on the effect of multiple, sequential intrasplenic transplants of fresh and cryopreserved hepatocytes in dalmatians.
  
  
  METHODS
  Dalmatians underwent intrasplenic hepatocyte transplantation with hepatocytes taken from healthy mongrels. Dalmatian urinary uric acid excretion was measured preoperatively, and this served as the control value. Three hepatocyte transplantations were performed at 30-day intervals--the first with freshly isolated cells, and both the second and the third with cryopreserved hepatocytes from the same donor. Urinary uric acid excretion was measured postoperatively twice per week.
  
  
  RESULTS
  The urinary uric acid excretion decreased an average of 54% after the first hepatocyte transplantation. The effect was transient and lasted an average of 22 days (range, 19-50 days). Subsequent intrasplenic hepatocyte transplantation with cryopreserved hepatocytes resulted in similar decreases in urinary uric acid excretion. Each transplant resulted in a significant decrease in urinary uric acid excretion when compared with baseline values (P = < .001).
  
  
  CONCLUSIONS
  Sequential intrasplenic hepatocyte transplantation is feasible in this model. This method provided a significant, but transient, correction in urinary uric acid excretion that was similar with either fresh or cryopreserved hepatocytes. A substantial biologic effect provided by cryopreserved hepatocytes has important implications in clinical hepatocyte transplantation.
authors.affiliation: Department of Surgery, University of Illinois at Chicago 60612, USA.
authors.forename: T B
authors.initials: TB
authors.lastname: Dunn
authors.forename: N H
authors.initials: NH
authors.lastname: Kumins
authors.forename: V
authors.initials: V
authors.lastname: Raofi
authors.forename: D M
authors.initials: DM
authors.lastname: Holman
authors.forename: M
authors.initials: M
authors.lastname: Mihalov
authors.forename: J
authors.initials: J
authors.lastname: Blanchard
authors.forename: W R
authors.initials: WR
authors.lastname: Law
authors.forename: C
authors.initials: C
authors.lastname: Rastellini
authors.forename: E
authors.initials: E
authors.lastname: Benedetti
mesh_terms: D000818:Animals; D002470:Cell Survival; D017690:Cell Transplantation; D015925:Cryopreservation; D004285:Dogs; D008099:Liver / Q000166:cytology*; D008107:Liver Diseases / Q000601:surgery; D008661:Metabolism, Inborn Errors / Q000601:surgery / Q000652:urine; D013154:Spleen; D016042:Transplantation, Heterotopic; D014527:Uric Acid / Q000652:urine
chemical_list: D014527:Uric Acid