P(1),P(4)-Diadenosine 5'-tetraphosphate modulates l-arginine and l-citrulline uptake by bovine aortic endothelial cells.
We have previously demonstrated that P(1),P(4)-diadenosine 5'-tetraphosphate (Ap(4)A) interacts with high-affinity and low-affinity binding sites on the bovine aortic endothelial cell (BAEC) surface. In this report we demonstrate that Ap(4)A interaction with the lower affinity site modulates l-arginine (l-Arg) and l-citrulline (l-Cit) uptake by BAEC. Competition uptake studies demonstrate that l-Arg and l-Cit uptake occurs through a common transporter system that is sensitive to Ap(4)A. Evidence is also presented that is consistent with Ap(4)A modulating l-Arg uptake by increasing the affinity of l-Arg for the transporter.
Hilderman
R H
RH
Department of Microbiology, Clemson University, Clemson, South Carolina, 29634-1903, USA. hilderr@clemson.edu
Casey
T E
TE
Pojoga
L H
LH
D000818:Animals; D001011:Aorta / Q000166:cytology; D001120:Arginine / Q000378:metabolism* / Q000493:pharmacokinetics; D001665:Binding Sites; D001667:Binding, Competitive / Q000187:drug effects; D001692:Biological Transport / Q000187:drug effects; D002417:Cattle; D002478:Cells, Cultured; D002956:Citrulline / Q000378:metabolism* / Q000493:pharmacokinetics; D015226:Dinucleoside Phosphates / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D013379:Substrate Specificity; D013498:Suramin / Q000494:pharmacology
D015226:Dinucleoside Phosphates; D011956:Receptors, Cell Surface; D018048:Receptors, Purinergic P2; C080426:adenosine 5',5''',P1,P4-tetraphosphate receptor; D002956:Citrulline; C020733:diadenosine tetraphosphate; D013498:Suramin; D001120:Arginine