[The changes of glutamate receptor and free Ca2+i in hypoxic-ischemic cerebral injury: experimental study].
This experiment was designed to explore the pathogenesis in hypoxic-ischemic encephalopathy (HIE). Sixteen newborn pigs were divided into two groups: (Group A) normal control and (Group B) HIE 24 hours. The glutamate receptor (Glu R) in forebrain crude synaptic membrane (SPM) and free Ca2+i in RBC were tested respectively. The results revealed that the binding sites (Bmax) of Glu R in Group B was much lower than that in Group A, but the affinity (Kd) showed no statistic difference between Group A and Group B. In addition, free Ca2+i of RBC in Group B was much higher than that in Group A. This study demonstrates that the changes of Glu R and Ca2+i are involved in the pathogenesis of hypoxic-ischemic cerebral injury in newborn animals.
Li
W
W
Pediatric Department, Second Affiliated Hospital, Chengdu.
Yao
Y
Y
Mu
D
D
Zhou
M
M
Tang
S
S
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism*; D017470:Receptors, Glutamate / Q000378:metabolism*; D013552:Swine; D013570:Synaptic Membranes / Q000378:metabolism*
D017470:Receptors, Glutamate; D002118:Calcium