Heterogeneous behavior of anti-beta2-glycoprotein I antibodies on various commercially available enzyme immunoassay plates coated with beta2-glycoprotein I.
OBJECTIVE
To evaluate commercially available enzyme immunoassay (EIA) plates for the measurement of anti-beta2-glycoprotein I autoantibody (anti-beta2-GPI).


METHODS
Sera from 10 patients with the antiphospholipid syndrome, and 3 monoclonal anti-beta2-GPI antibodies established from patients with antiphospholipid syndrome, were assayed for binding to solid phase beta2-GPI on 20 commercially available plates.


RESULTS
Several commercially available EIA plates were found to be of potential value for the measurement of anti-beta2-GPI autoantibody. Some plates were unsuitable for anti-beta2-GPI detection, possibly due to less beta2-GPI on the plates, or to differences in the nature of conformational changes of beta2-GPI induced by the plates.


CONCLUSIONS
Differences among EIA plates need to be considered when measuring beta2-GPI antibodies.
Tsutsumi
A
A
Department of Medicine II, Hokkaido University School of Medicine, Sapporo, Japan.
Ichikawa
K
K
Matsuura
E
E
Sawada
K I
KI
Koike
T
T
D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D053482:beta 2-Glycoprotein I
D001323:Autoantibodies; D006023:Glycoproteins; D053482:beta 2-Glycoprotein I