HPMA copolymer-modified avidin: immune response.
Protein-polymer conjugates to be used in the pretargeted delivery of a photosensitizer to cells were synthesized and characterized. Avidin was modified by N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers bearing the photosensitizer, mesochlorin e6 mono(N-2-aminoethylamide) (Mce6). Synthesis of HPMA copolymer-avidin-Mce6 conjugates was carried out so that either predominantly single point attachment or multipoint attachment of copolymer chains to avidin would result. HPMA copolymer-avidin conjugates were used which retained specific binding activity to a lower affinity biotin analog. Antigen specific anti-avidin immune response was shown to be reduced six-fold in some HPMA copolymer-avidin conjugates when compared to immune response to unmodified avidin. HPMA copolymer itself was shown to elicit a very low (IgM) immune response.
Hart
P R
PR
Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City 84112, USA.
Kopecková
P
P
Omelyanenko
V
V
Enioutina
E
E
Kopecek
J
J
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000918:Antibody Specificity; D001360:Avidin / Q000276:immunology* / Q000378:metabolism; D016503:Drug Delivery Systems; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008689:Methacrylates / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D017319:Photosensitizing Agents / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis* / Q000378:metabolism
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008689:Methacrylates; D017319:Photosensitizing Agents; D011108:Polymers; D001360:Avidin; C032802:hydroxypropyl methacrylate