Fluorescent automated single-stranded conformation (F-SSCP) analysis is able to detect a point mutation at the extreme 5' end of a PCR product.
Pirie
F J
FJ
Department of Medicine, University of Natal, Durban, South Africa. pirie@med.und.ac.za
York
D F
DF
Motala
A A
AA
Omar
M A
MA
D004252:DNA Mutational Analysis / Q000379:methods*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity
D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases