Localization of glyoxylate dehydrogenase and glyoxylate-complex molecules in the rat prefrontal cortex: enzymohistochemical and immunocytochemical study.
Glyoxylic acid is synthesized and catabolized in cells of vertebrates; several pathways have been described. In previous papers, we have demonstrated the localization in some areas of the rat cerebral cortex both of beta-NAD-dependent glyoxylate dehydrogenase (glyoDH), using an enzymohistochemical method, and of glyoxylate-complex molecules, using immunocytochemical procedures. In this study we have applied these two techniques in various areas of the prefrontal cortex with different histological cytoarchitecture. GlyoDH has been located in most neurons, in some glial cells, and in capillary wall structures in all cortical layers of all areas of the rat prefrontal cortex. Antibodies against glyoxylate-complex molecules showed positive immunoreactivity in scattered neurons, mostly of multipolar or stellate appearance, from layers III, IV, and V in the medial precentral area, but not in cortical areas 24, 25, or 32 of the prefrontal cortex. Immunoreaction was found in the periphery of neuronal perikarya and in some of their processes. These results demonstrate the existence of a particular area-dependent neuronal cortical system, of specific but uncertain function, related to glyoxylic acid and/or glyoxylate compounds. At the electron microscope level, positive reaction was associated with synaptic sites, axonal filaments, glial cells, and several components of the blood-brain barrier. These localizations suggest the involvement of glyoxylate derivatives in synaptic functioning and also in glial cell functions.
Gragera
R R
RR
Department of Morphological Sciences and Surgery, Faculty of Medicine, Alcalá de Henares University, Alcalá de Henares, Spain.
Martínez-Rodríguez
R
R
Capilla
J
J
De Miguel
E
E
Gómez de Segura
I
I
Turégano
L
L
Alvarez
M I
MI
Toledano
A
A
D000445:Aldehyde Oxidoreductases / Q000032:analysis*; D000818:Animals; D001253:Astrocytes / Q000737:chemistry*; D006038:Glyoxylates / Q000032:analysis*; D006651:Histocytochemistry; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009474:Neurons / Q000737:chemistry*; D017397:Prefrontal Cortex / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D006038:Glyoxylates; D009243:NAD; D009249:NADP; D000445:Aldehyde Oxidoreductases; C040590:glyoxylate dehydrogenase (acylating); C031150:glyoxylic acid