Complete sequence and characterization of chick ventricular myosin heavy chain in the developing atria.
We isolated five complementary DNA (cDNA) clones, encoding the chick ventricular myosin heavy chain (MyHC) by reverse transcription polymerase chain reaction (RT-PCR). The entire cDNA consists of 5995 nucleotides with the 52 bp 5'-untranslated region and the 129 bp 3'-untranslated region. The complete cDNA encodes 1937 amino acids. Expression of the chick ventricular MyHC gene was also studied by Northern blot analysis. This gene continued to be strongly expressed in the ventricle during cardiac development. On the other hand, its expression was moderate in the early embryonic atria, and was down-regulated during development. In the adult atria, this gene was expressed at very low levels. To determine the localization of the ventricular MyHC protein, an immunohistochemical study was performed. The ventricular MyHC was present in early embryonic atrial myocytes. During development, the expression of this protein in the atrial myocytes was down-regulated, but continued to be present in the atrial conduction system. Our results indicate that the ventricular MyHC appears in the primary atrial myocardium and is then localized in the conduction cells of the atria.
Machida
S
S
Department of Pediatric Cardiology, The Heart Institute of Japan, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku, Tokyo, Japan.
Noda
S
S
Furutani
Y
Y
Takao
A
A
Momma
K
K
Matsuoka
R
R
D000818:Animals; D015152:Blotting, Northern; D002642:Chick Embryo; D002645:Chickens; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D006329:Heart Conduction System / Q000378:metabolism; D006352:Heart Ventricles / Q000196:embryology / Q000254:growth & development; D007150:Immunohistochemistry; D009206:Myocardium / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D012333:RNA, Messenger; D018995:Myosin Heavy Chains