Neutralization of the hemorrhagic activity of Bothrops and Lachesis snake venoms by a monoclonal antibody against mutalysin-II.
One mAb reactive with mutalysin-II, a hemorrhagic metalloproteinase isolated from Lachesis muta muta venom, was produced in mice immunized with L. m. muta venom. Indirect ELISA was employed to compare the antigenic cross-reactivity among the venoms from Bothrops snakes. The mAb anti-mutalysin-II efficiently neutralized the hemorrhagic effect of both mutalysin-II and L. m. muta crude venom. Furthermore, the mAb were cross-reactive with B. alternatus, B. atrox, B. itapetiningae, B. jararaca and B. neuwiedii and showed variable potencies in neutralizing the hemorrhagic activity of several bothropic venoms.
Estêvão-Costa
M I
MI
Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
Martins
M S
MS
Sánchez
E F
EF
Diniz
C R
CR
Chávez-Olórtegui
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017837:Bothrops; D002459:Cell Fusion / Q000187:drug effects; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006470:Hemorrhage / Q000139:chemically induced* / Q000517:prevention & control*; D008297:Male; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D014757:Viper Venoms / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D017819:Viperidae
D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D014757:Viper Venoms; D008666:Metalloendopeptidases; C068795:hemorrhagic factor LHFII