Interleukin-13 prevents diaphragm muscle deterioration in a septic animal model.
The effects of an intravenous injection of Interleukin-13 (IL-13) after endotoxin administration on diaphragm muscle were studied using Wistar rats. Two treatment groups, a control (saline+endotoxin) group and an IL-13 (IL-13+endotoxin) group were studied. E. coli endotoxin (10 mg/kg) was injected intraperitoneally 5 minutes after saline or IL-13 (0.25 microg) injection. The force-frequency curves, twitch kinetics and fatigability were measured at 0 and 4 hours after endotoxin injection. The force-frequency curves and twitch tension in the control group were significantly lower at 4 hours than those at 0 hour due to endotoxin. On the other hand, IL-13 prevented the decrement of the force-frequency curves and twitch tension induced by endotoxin. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry showed positive staining at 4 hours due to endotoxin in the control group; however, IL-13 also blocked NADPH diaphorase staining at 4 hours. Furthermore, the positive muscle fibers detected by the NADPH diaphorase staining were classified as type I (slow twitch) muscle fibers by ATPase staining. We conclude that IL-13 prevents the deterioration of contraction induced by endotoxin by inhibiting nitric oxide production in the diaphragm muscle, mainly the type I muscle fibers.
Takahashi
Y
Y
Department of Medical Technology, College of Medical Sciences, Tohoku University, Sendai, Japan.
Katayose
D
D
Shindoh
C
C
D000818:Animals; D002298:Cardenolides; D003964:Diaphragm / Q000473:pathology / Q000503:physiopathology; D004077:Digoxin; D018793:Interleukin-13 / Q000494:pharmacology* / Q000627:therapeutic use; D009119:Muscle Contraction / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000633:toxicity; D018805:Sepsis / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology
D002298:Cardenolides; D018793:Interleukin-13; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin