Injection of plasmid DNA into the gastric mucosa induces mucosal and systemic immunity.
Nearly all mucosal surfaces participate in a common mucosal immune system, and application of an antigen to one mucosal surface elicits local as well as distant mucosal immune responses. However, whether the gastric mucosa is a part of this network has not been examined directly. We show here that the injection of plasmid DNA encoding beta-galactosidase into the gastric wall caused transfection of gastric mucosal epithelial cells, induced systemic and mucosal antibody responses at both local (digestive tract) and distant (genital and respiratory tracts) sites, and induced cytotoxic T lymphocyte responses in the spleen and the mesenteric and iliac lymph nodes.
Sato
Y
Y
Department of Internal Medicine II, Fukushima Medical University School of Medicine, Fukushima, 960-1295, Japan. med2fmc@po.sphere.ne.jp
Saito
A
A
Shishido
H
H
Irisawa
A
A
Miyata
M
M
Obara
K
K
Nishimaki
T
T
Fujita
T
T
Suzuki
T
T
Kasukawa
R
R
D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression; D018928:Immunity, Mucosal / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis / Q000097:blood* / Q000276:immunology; D008099:Liver / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014621:Vagina / Q000276:immunology; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D000941:Antigens; D007136:Immunoglobulins; D019444:Vaccines, DNA; D001616:beta-Galactosidase