[The effect of a vaccine made from 39kd hydrophobic outer membrane protein of Leptospira interrogans on neurohumoral and red cell immunity function of the guinea pigs].
A randomized control trial was conducted to determine the immunoprotective efficacy of OmpL39. 36 guinea pigs were divided into OmpL39 group, whole leptospiral cell vaccine (WLCV) group, other proteins of Leptospira group, and negative control group (normal saline, NS). The results showed that all the guinea pigs of infected OmpL39 and WLCV still survived, but all the control guinea pigs died. Immunoprotective efficacy was 100% for OmpL39 and WLCV. OmpL39 levels were similar to WLCV levels and higher than controls (P < 0.05). These suggested that OmpL39 could be used as important immunoprotective antigen to develop the genetic vaccine of the targeting delivery system. Also it was observed that OmpL39 could produce 100% immunoprotective efficacy, have higher MAT level (> 1:3200) and regulate 5-HT, 5-HIAA, DA, NE. The 5-HT, DA, NE concentration was lower and 5-HIAA was higher than that of controls after stimulating the guinea pigs with OmpL39 (P < 0.05). The results suggested that OmpL39 genetic vaccine could produce immunity function and have an active effect on absorbing inflammation and protecting organs and tissues. Besides, the red cell immunity efficacy of the guinea pigs was changed during immunity response and the RBC-C3b RR and the RBC-ICR were increased. The RFER was increased; the RFIR was decreased. RFER/RFIR was remarkably higher than that of control (P < 0.05). These suggested that OmpL39 could increase red cell immunity function.
Zhang
Y
Y
Department of Obs/Gyn, Second Affiliated Hospital, Chengdu.
Qie
M
M
Zeng
X
X
Cheng
J
J
Wu
J
J
Liu
J
J
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification; D015306:Biogenic Monoamines / Q000097:blood; D004912:Erythrocytes / Q000276:immunology*; D006168:Guinea Pigs; D007921:Leptospira interrogans / Q000737:chemistry / Q000276:immunology*; D011897:Random Allocation; D017463:Receptors, Complement 3b / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D001425:Bacterial Outer Membrane Proteins; D015306:Biogenic Monoamines; D017463:Receptors, Complement 3b; D019444:Vaccines, DNA