[Study on the molecular epidemiology characteristics of Pseudomonas cocovenenans subsp. farinofermentans isolated from China with rDNA fingerprinting].
After the chromosomal DNA digestion by Bgl II and EcoRV, rDNA fingerprinting for Pseudomonas cocovenenans subsp. farinofermentans was made using biotin-labeled 16S + 23S rDNA as a probe. Fifty one strains of P. cocovenenans subsp. farinofermentans isolated from some areas of China were divided into 20 ribotyes, 52.9 percent of which were RT6, 9 and 2. In most cases, there were 2 or more ribotypes in each province or city. Though same ribotypes were isolated from different provinces, each ribotype existed in part of an area. The ribotype of RT6 was detected in samples from 4 incidents of food poisoning in Guizhou, Jiangsu and Hebei provinces, which indicated that these incidents may be caused by the same clone. With discrimination and cluster analysis of ribotypes, the rDNA fingerprinting may effectively contribute to analyzing the homogeneity of different strains, tracing the transmission route, and exploring the evolution and epidemiological characteristics of P. cocovenenans subsp. farinofermentans.
Qiu
M
M
Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing, China.
Liu
X
X
Yang
R
R
D002681:China / Q000453:epidemiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D017720:Molecular Epidemiology; D011549:Pseudomonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D011552:Pseudomonas Infections / Q000453:epidemiology*
D004269:DNA, Bacterial