The human immunoglobulin loci introduced into mice: V (D) and J gene segment usage similar to that of adult humans.
Variable gene segments of the human immunoglobulin loci are represented in the human peripheral repertoire at different frequencies. XenoMouse strains contain approximately 2 megabases of the human immunoglobulin heavy and kappa light chain loci that functionally recapitulate the human humoral immune system. Analysis of human antibody transcripts from XenoMouse spleens and lymph nodes revealed that V, D and J gene segment utilization from these unimmunized animals were nearly identical to the gene segment utilization reported for humans with extensive antigenic histories.
Gallo
M L
ML
Abgenix, Inc., Fremont, CA 94555, USA. gallo_m@abgenix.com
Ivanov
V E
VE
Jakobovits
A
A
Davis
C G
CG
D000328:Adult; D000818:Animals; D018014:Gene Transfer Techniques; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics* / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic; D014158:Transcription, Genetic
D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains