title: A common high molecular weight antigen of Babesia bovis isolates from Mexico.
abstract: Cattle from an area of Mexico endemic with Babesia bovis infections have a dominant antibody response to a 152kDa antigen of the Tamaulipas strain of B. bovis. A mAb termed PB/5, showing a specific reactivity to this 152kDa antigen in Western blots, was identified. The mAb which reacted with the blunt end of B. bovis in an indirect fluorescent antibody test also reacted to a 152kDa antigen in two other isolates (Nuevo Leon and Yucatan), and a 175kDa antigen in the Huasteca B. bovis isolate from Mexico. Polyclonal monospecific sera from a calf inoculated with mAb-affinity purified 152kDa antigen (Tamaulipas strain) identified B. bovis by the indirect fluorescent antibody test and two antigens of B. bovis (65kDa and 152kDa) in Western blot. Since the epitope reacting to the mAb PB/5 is conserved, this antigen provides a basis for developing a diagnostic test or an immunogen.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843-4467, USA.
authors.forename: A
authors.initials: A
authors.lastname: Sahagun Ruiz
authors.forename: S D
authors.initials: SD
authors.lastname: Waghela
authors.forename: M M
authors.initials: MM
authors.lastname: Romany
authors.forename: P J
authors.initials: PJ
authors.lastname: Holman
authors.forename: D
authors.initials: D
authors.lastname: Melendy
authors.forename: D
authors.initials: D
authors.lastname: Cruz
authors.forename: G G
authors.initials: GG
authors.lastname: Wagner
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D016793:Babesia bovis / Q000276:immunology*; D001404:Babesiosis / Q000453:epidemiology / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D016056:Immunodominant Epitopes; D008800:Mexico
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016056:Immunodominant Epitopes