The oocyte-specific methylated region of the U2afbp-rs/U2af1-rs1 gene is dispensable for its imprinted methylation.
Imprinted genes harbor discrete regions which are differentially methylated in gametes; usually the final differential methylation patterns in adults are established during embryogenesis through modifications of the initial methylation patterns in gametes. Previous reports have shown that a 200-bp region termed region II within the CpG island of the mouse imprinted U2afbp-rs gene is methylated in oocytes but not in sperm, suggesting that this region is a center for the propagation of methylated CpGs on the maternal allele and is also a candidate for an imprinting control element. To determine whether region II is required for the imprinted methylation of this gene at the endogenous locus, we generated mice carrying a deletion of this region. We herein show that parental methylation differences still exist in the CpG island on the region II-deleted allele. These findings suggest that region II is dispensable for the imprinted methylation of the U2afbp-rs gene.
Sunahara
S
S
Division of DNA Biology and Embryo Engineering, CREST, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Tokyo, Minato-ku, 108-8639, Japan.
Nakamura
K
K
Nakao
K
K
Gondo
Y
Y
Nagata
Y
Y
Katsuki
M
M
D000818:Animals; D019175:DNA Methylation; D018390:Gene Targeting; D018392:Genomic Imprinting; D051379:Mice; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D009865:Oocytes / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics* / Q000502:physiology; D000072668:Splicing Factor U2AF
D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D000072668:Splicing Factor U2AF; C000607495:Zrsr1 protein, mouse