title: Crystallization and preliminary X-ray analysis of recombinant full-length human m-calpain.
abstract: m-Calpain constitutes the prototype of the superfamily of neutral calcium-activated cysteine proteinases. It is a heterodimer consisting of an 80 and a 30 kDa subunit. Recombinant full-length human m-calpain has been crystallized using macro-seeding techniques and vapour-diffusion methods. Two different monoclinic crystal forms (space group P2(1)) were obtained from a solution containing polyethylene glycol (M(W) = 10 000) as a precipitating agent. Complete data sets have been collected to 2.3 and 3.0 A resolution using cryo-cooling conditions and synchrotron radiation. The unit-cell parameters are a = 64.86, b = 133.97, c = 78.00 A, beta = 102.43 degrees and a = 51.80, b = 171.36, c = 64.66 A, beta = 94.78 degrees, respectively. The V(m) values indicate that there is one heterodimer in each asymmetric unit.
authors.affiliation: Laboratory of Molecular Structure and Function, Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1--1--1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Masumoto
authors.forename: K
authors.initials: K
authors.lastname: Nakagawa
authors.forename: S
authors.initials: S
authors.lastname: Irie
authors.forename: H
authors.initials: H
authors.lastname: Sorimachi
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: G P
authors.initials: GP
authors.lastname: Bourenkov
authors.forename: H
authors.initials: H
authors.lastname: Bartunik
authors.forename: C
authors.initials: C
authors.lastname: Fernandez-Catalan
authors.forename: W
authors.initials: W
authors.lastname: Bode
authors.forename: S
authors.initials: S
authors.lastname: Strobl
mesh_terms: D002154:Calpain / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008970:Molecular Weight; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D002154:Calpain