[Significance of glycosaminoglycans content in developing mechanism of cleft palate].
To explore significance of Glycosaminoglycans content in the development of embryo and forming mechanism of cleft palate, Dexamethason acetate was injected into the abdomen of NIH mouse on the 12th day of gestation to induce cleft palate mouse embryo model. Then by taking cranio-maxillary coronary sections, stained with Alcian blue/PAS and quantitatively analysing the area of the palatal process covered by glycosaminoglycans using a TDS-90 photodigital system with computer. Results indicate that on 13(12) day(hour) of gestation the colour of Alcian blue starts to show in the palatal process. By then, the area covered by glycosaminoglycans in the normal group is 4.69 mm whereas for the experimental group is 2.40 mm, showing a significant statistical difference (P < 0.05). The area of the palatal process covered by glycosaminoglycans gradually increases as the embryo developes, but is distinctly smaller in experimental than in the normal group (P > 0.05). This proves that there's a relationship between the level of glycosaminoglycans in the palatal process and development of cleft palates.
Fu
Y
Y
School of Stomatology, Hubei Medical University, Wuhan.
Li
Z
Z
Huang
H
H
D000818:Animals; D002972:Cleft Palate / Q000139:chemically induced / Q000196:embryology / Q000378:metabolism*; D003907:Dexamethasone / Q000031:analogs & derivatives; D005314:Embryonic and Fetal Development; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D008297:Male; D051379:Mice; D010159:Palate / Q000378:metabolism*
D006025:Glycosaminoglycans; D003907:Dexamethasone; C018038:dexamethasone acetate