Microinfusion of protein kinase inhibitor H7 into the cerebellum impairs the acquisition but not the retention of classical eyeblink conditioning in rabbits.
Rabbits were infused with H7, a general protein kinase inhibitor, into the region of the cerebellar interpositus nucleus during classical eyeblink conditioning. Acquisition of the conditioned eyeblink response was delayed by the H7 infusion, but the protein kinase inhibitor had no effect on performance of the learned response when infused after asymptotic learning had been reached. These data indicate that protein kinases in the cerebellum are involved in plasticity processes that underlie the learning of this simple conditioned behavior.
Chen
G
G
Program in Neural Science, Indiana University, Bloomington, IN, USA.
Steinmetz
J E
JE
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001767:Blinking / Q000187:drug effects* / Q000502:physiology; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D003214:Conditioning, Classical / Q000187:drug effects* / Q000502:physiology; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D007263:Infusions, Parenteral; D007858:Learning; D008297:Male; D009118:Muscimol / Q000008:administration & dosage / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011817:Rabbits
D004791:Enzyme Inhibitors; D047428:Protein Kinase Inhibitors; D009118:Muscimol; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine