title: Expression, crystallization and preliminary X-ray studies of the PDZ domain of Dishevelled protein.
abstract: Dishevelled (Dsh) protein is an important component of the Wnt signal-transduction pathway. It has three relatively conserved domains: DIX, PDZ and DEP. The PDZ domain of the Xenopus laevis homolog of Dsh, which consists of residues 254-348, was overexpressed as a soluble protein in Escherichia coli, purified and crystallized. The crystals were obtained by the vapor-diffusion method, using 1.4 M sodium formate as a precipitant. The crystals diffracted to 2.3 A resolution. The space group was determined to be P6(1)22 or P6(5)22, with unit-cell dimensions a = b = 95.9, c = 93.9 A.
authors.affiliation: Macromolecular Crystallography Facility at the Advanced Light Source, Physical Biosciences Division, MS 6-2100, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: N
authors.initials: N
authors.lastname: Khlebtsova
authors.forename: L W
authors.initials: LW
authors.lastname: Hung
authors.forename: K
authors.initials: K
authors.lastname: Henderson
authors.forename: R
authors.initials: R
authors.lastname: Moon
authors.forename: T
authors.initials: T
authors.lastname: Earnest
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D000072261:Dishevelled Proteins; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C000606586:DVL1 protein, Xenopus; D000072261:Dishevelled Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D029867:Xenopus Proteins