Use of the confocal microscope to determine polyomavirus recombinant capsid-like particle entry into mouse 3T6 cells.
The structural protein genes of polyomavirus were expressed in the baculovirus system, and the proteins were found to assemble into capsid-like particles capable of packaging insect cell DNA. Recombinant capsid-like particles could be produced that were composed of the various structural proteins (VP1, VP1/2, VP1/3 and VP1/2 + VP3). Laser scanning confocal microscopy was used to determine if the various capsid-like particles could infect (enter) mouse 3T6 cells. Each of the various capsid-like particles was equally capable of cell entry as determined by indirect immunofluorescence confocal microscopy.
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Division of Biology-Ackert Hall, Section of Virology and Oncology, Kansas State University, Manhattan 66506, USA.
Lovgren
T R
TR
Tilley
M B
MB
Consigli
R A
RA
D000818:Animals; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D011120:Polyomavirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000378:metabolism*
D011994:Recombinant Proteins; D015678:Viral Structural Proteins