title: Effects of tetrandrine on changes of NMDA receptor channel in cortical neurons of rat induced by anoxia.
abstract: OBJECTIVE
  To study the effects of tetrandrine (Tet) on the changes of NMDA receptor channels in cortical neurons induced by anoxia.
  
  
  METHODS
  Cell-attached configuration of patch-clamp techniques. Anoxia was produced by perfused cells with 95% N2 + 5% CO2 gassed bath solution.
  
  
  RESULTS
  During anoxia, the open time constant (tau 2), open probability (Po) of 35-pS and 100-pS channels increased. Tet 7.5 mumol.L-1 reduced the Po of 35-pS and 100-pS channels, 15 and 30 mumol.L-1 inhibited open of 100-pS channel fully, and changed the open time constant of 35-pS from two to single exponential distribution.
  
  
  CONCLUSIONS
  Tet inhibition of the open of NMDA receptor channels induced by anoxia was one of its protective mechanisms.
authors.affiliation: Department of Pharmacology, Chongqing University of Medical Sciences, China. zfwang@sunm.shcnc.ac.cn
authors.forename: Z F
authors.initials: ZF
authors.lastname: Wang
authors.forename: C S
authors.initials: CS
authors.lastname: Xue
authors.forename: Q X
authors.initials: QX
authors.lastname: Zhou
authors.forename: Z B
authors.initials: ZB
authors.lastname: Wan
authors.forename: Q S
authors.initials: QS
authors.lastname: Luo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015687:Cell Hypoxia; D002469:Cell Separation; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects*
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D002121:Calcium Channel Blockers; D018696:Neuroprotective Agents; D016194:Receptors, N-Methyl-D-Aspartate; C009438:tetrandrine