Heart fatty acid binding protein and cardiac troponin T plasma concentrations as markers for myocardial infarction after coronary artery ligation in mice.
Ligation of the main left coronary artery in mice serves as a model for myocardial infarction (MI). We tested whether plasma concentrations of heart-type fatty acid-binding protein (H-FABP) and/or cardiac troponin T (cTnT) discriminate between infarcted and sham-operated mice and allow estimation of infarct size. Mice were subjected to coronary artery ligation or sham surgery and release curves of H-FABP and cTnT were determined. At 4 h after surgery the mean (+/-SD) H-FABP plasma concentration was 461+/-134 microg/l (n=10) in MI and 185+/-51 microg/l (n=6; P<0.001) in sham-operated mice. By 24 h after surgery H-FABP levels had returned to normal in both groups. cTnT plasma concentrations increased up to 48 h after MI to 13.5+/-6.2 microg/l (n=6; P<0.001) compared with 0.031+/-0.063 microg/l (n=7) in sham-operated mice. Linear regression analysis revealed a significant correlation between plasma H-FABP at 4 h and infarct size assessed 7 days after surgery. Plasma cTnT did not correlate significantly with infarct size. In conclusion, plasma cTnT concentration at 48 h after infarction can be used to distinguish MI from sham mice, whereas H-FABP concentration at 4 h can be used for stratification of animals according to infarct size.
Aartsen
W M
WM
Department of Pharmacology and Toxicology, Cardiovascular Research Institute Maastricht, Universiteit Maastricht, The Netherlands. wendy.aartsen@farmaco.unimaas.nl
Pelsers
M M
MM
Hermens
W T
WT
Glatz
J F
JF
Daemen
M J
MJ
Smits
J F
JF
D000818:Animals; D015415:Biomarkers; D002352:Carrier Proteins / Q000097:blood*; D003331:Coronary Vessels; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D008026:Ligation; D008297:Male; D051379:Mice; D018996:Myelin P2 Protein / Q000097:blood*; D009203:Myocardial Infarction / Q000097:blood / Q000473:pathology*; D017202:Myocardial Ischemia / Q000097:blood / Q000473:pathology; D009206:Myocardium / Q000473:pathology*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011237:Predictive Value of Tests; D020107:Troponin T / Q000097:blood*
D015415:Biomarkers; D002352:Carrier Proteins; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D020107:Troponin T