title: Kinetics of inhibition of green crab (Scylla serrata) alkaline phosphatase by sodium (2,2'-bipyridine) oxodiperoxovanadate.
abstract: Green crab (Scylla serrata) alkaline phosphatase (EC 3.1.3.1) is a metalloenzyme, which catalyzes the nonspecific hydrolysis of phosphate monoesters. The kinetics of inhibition of the enzyme by sodium (2, 2'-bipyridine) oxodiperoxovanadate, pV(bipy), has been studied. The time course of the hydrolysis of p-nitrophenyl-phosphate catalyzed by the enzyme in the presence of different pV(bipy) concentrations showed that at each pV(bipy) concentration, the rate decreased with increasing time until a straight line was approached, the straight line slopes are the same for all concentrations. The results suggest that the inhibition of the enzyme by pV(bipy) is a slow, reversible reaction with fractional remaining activity. The microscopic rate constants are determined for the reaction of inhibitor with the enzyme.
authors.affiliation: Department of Biology, Xiamen University, PR China.
authors.forename: X W
authors.initials: XW
authors.lastname: Zhou
authors.forename: Z L
authors.initials: ZL
authors.lastname: Zhuang
authors.forename: Q X
authors.initials: QX
authors.lastname: Chen
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D003386:Brachyura / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D009942:Organometallic Compounds / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D009942:Organometallic Compounds; C405515:oxodiperoxo(2,2'-bipyridine)vanadate(V); D015082:2,2'-Dipyridyl; D000469:Alkaline Phosphatase