Alkaline treatment after X-Gal staining reaction for Escherichia coli beta-galactosidase enhances sensitivity.
Tanahashi
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Division of Demyelinating Disease and Aging, National Institute of Neuroscience, 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan. tanahash@ncnp.go.jp
Tabira
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T
D000468:Alkalies; D002863:Chromogenic Compounds; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005697:Galactosides; D007211:Indoles; D007763:Lac Operon; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*; D013379:Substrate Specificity; D014162:Transfection; D001616:beta-Galactosidase / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D000468:Alkalies; D002863:Chromogenic Compounds; D005697:Galactosides; D007211:Indoles; D011994:Recombinant Proteins; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside