Association of functional mutant homozygotes of the mannose binding protein gene with susceptibility to pulmonary tuberculosis in India.
METHODS
Mannose binding protein gene polymorphism in pulmonary tuberculosis in India.


OBJECTIVE
To find out whether non-HLA genes such as mannose binding protein (MBP) genes are associated in the susceptibility to pulmonary tuberculosis.


METHODS
Genotyping of MBP 52, 54 and 57 wild and mutant alleles was carried out in HLA-DR typed pulmonary tuberculosis patients (n = 202) and control subjects (n = 109). Since HLA-DR2 is associated with pulmonary-TB, the interaction of MBP genes on -DR2 and non-DR2 genes on the susceptibility was also studied.


RESULTS
A significantly increased genotype frequency of MBP functional mutant homozygotes (including 52, 54 and 57) was seen in pulmonary tuberculosis (PTB) patients (10.9%) than in control subjects (1.8%; P = 0.008; odds ratio: 6.5). Analysis of interaction of MBP genes and HLA-DR2 on the susceptibility to PTB revealed that these genes are associated with PTB independent of each other.


CONCLUSIONS
The present study shows that functional mutants of MBP are associated with PTB. Apart from HLA-DR2 association, association of non-HLA genes in the susceptibility to PTB is evident. This suggests that multigenetic factors (candidate genes) may be involved in the susceptibility/resistance to PTB.
Selvaraj
P
P
Tuberculosis Research Centre (ICMR), Chennai, India. icmrtrc@ren.nic.in/trcicmr@giasmd01.vsnl.net.in
Narayanan
P R
PR
Reetha
A M
AM
D000328:Adult; D002352:Carrier Proteins / Q000235:genetics*; D037222:Collectins; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015802:HLA-DR2 Antigen / Q000235:genetics; D006720:Homozygote; D006801:Humans; D007194:India; D008297:Male; D009154:Mutation / Q000235:genetics*; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000276:immunology
D002352:Carrier Proteins; D037222:Collectins; D015802:HLA-DR2 Antigen