Search for a novel killer toxin in yeast Pichia pastoris.
Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have been identified. In the present study, the search for toxin-producing capability in P. pastoris has been conducted. No killer activity could be detected when 14 different indicator strains were tested.
Banerjee
H
H
Department of Biology, Long Island University, Brooklyn Campus, New York, 11201-8423, USA.
Verma
M
M
D001758:Blastomyces / Q000187:drug effects; D002175:Candida / Q000187:drug effects; D003470:Culture Media / Q000633:toxicity; D055646:Killer Factors, Yeast; D007716:Kluyveromyces / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods; D009183:Mycotoxins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity*; D010843:Pichia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D010957:Plasmids / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity
D003470:Culture Media; D055646:Killer Factors, Yeast; D009183:Mycotoxins; C058975:zymocin; C087906:killer toxin, Pichia