Determination of natural versus laboratory human infection with Mayaro virus by molecular analysis.
A laboratory worker developed clinical signs of infection with Mayaro virus (Togaviridae), an arbovirus of South and Central America, 6 days after preparation of Mayaro viral antigen and 10 days after a trip to a rain forest. There was no evidence of skin lesions during the antigen preparation, and level 3 containment safety measures were followed. Therefore, molecular characterization of the virus was undertaken to identify the source of infection. RT-PCR and DNA sequence comparisons proved the infection was with the laboratory strain. Airborne Mayaro virus contamination is thus a hazard to laboratory personnel.
Junt
T
T
Centre National de Référence pour la Surveillance des Arboviroses dans la Région Antilles-Guyane, Institut Pasteur de la Guyane, Cayenne, French Guiana.
Heraud
J M
JM
Lelarge
J
J
Labeau
B
B
Talarmin
A
A
D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D019570:Inhalation Exposure; D017056:Medical Laboratory Personnel; D008969:Molecular Sequence Data; D016273:Occupational Exposure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014035:Togaviridae / Q000235:genetics* / Q000472:pathogenicity; D014036:Togaviridae Infections / Q000235:genetics / Q000635:transmission*
D004279:DNA, Viral