Restriction-fragment-length polymorphism analysis of small-subunit rRNA genes of Blastocystis isolates from animal hosts.
The anaerobic enteric protozoan organism Blastocystis sp. has been identified from mammalian, avian, reptilian, and arthropod hosts. Eight Blastocystis isolates from five animal host species (cow, goat, sheep, guinea pig, and rhea) were compared by small-subunit ribosomal RNA (ssu rRNA) restriction-fragment-length polymorphism (RFLP) analyses using five restriction endonucleases. The isolates sorted into five genotypes. Multiple genotypes were found in isolates from a single animal host species, and multiple host species shared a single genotype. A molecular method such as RFLP analysis of ssu rRNA genes facilitates the characterization of Blastocystis isolates from various host species.
Snowden
K
K
College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
Logan
K
K
Blozinski
C
C
Hoevers
J
J
Holman
P
P
D000818:Animals; D016844:Blastocystis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016776:Blastocystis Infections / Q000469:parasitology / Q000662:veterinary; D002417:Cattle; D015252:Deoxyribonucleases, Type II Site-Specific; D020459:Genes, rRNA / Q000235:genetics*; D006168:Guinea Pigs; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D012756:Sheep / Q000469:parasitology
D012335:RNA, Ribosomal; D015252:Deoxyribonucleases, Type II Site-Specific