Cytochrome c' from Rhodobacter capsulatus confers increased resistance to nitric oxide.
We report the cloning and sequencing of the gene containing cytochrome c' (cycP) from the photosynthetic purple bacterium Rhodobacter capsulatus and the regions flanking that gene. Mutant strains unable to synthesize cytochrome c' had increased sensitivity to nitrosothiols and to nitric oxide (which binds to the heme moiety of cytochrome c').
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Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Aish
J
J
Paston
S J
SJ
Poole
R K
RK
Moir
J W
JW
D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000235:genetics / Q000502:physiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004352:Drug Resistance, Microbial; D004579:Electron Transport / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009569:Nitric Oxide / Q000494:pharmacology*; D010100:Oxygen / Q000493:pharmacokinetics; D016354:Rhodobacter capsulatus / Q000187:drug effects* / Q000235:genetics; D017422:Sequence Analysis, DNA
D003574:Cytochrome c Group; D004269:DNA, Bacterial; D009569:Nitric Oxide; D010100:Oxygen