title: The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells.
abstract: The mouse SKD1 is an AAA-type ATPase homologous to the yeast Vps4p implicated in transport from endosomes to the vacuole. To elucidate a possible role of SKD1 in mammalian endocytosis, we generated a mutant SKD1, harboring a mutation (E235Q) that is equivalent to the dominant negative mutation (E233Q) in Vps4p. Overexpression of the mutant SKD1 in cultured mammalian cells caused defect in uptake of transferrin and low-density lipoprotein. This was due to loss of their receptors from the cell surface. The decrease of the surface transferrin receptor (TfR) was correlated with expression levels of the mutant protein. The mutant protein displayed a perinuclear punctate distribution in contrast to a diffuse pattern of the wild-type SKD1. TfR, the lysosomal protein lamp-1, endocytosed dextran, and epidermal growth factor but not markers for the secretory pathway were accumulated in the mutant SKD1-localized compartments. Degradation of epidermal growth factor was inhibited. Electron microscopy revealed that the compartments were exaggerated multivesicular vacuoles with numerous tubulo-vesicular extensions containing TfR and endocytosed horseradish peroxidase. The early endosome antigen EEA1 was also redistributed to these aberrant membranes. Taken together, our findings suggest that SKD1 regulates morphology of endosomes and membrane traffic through them.
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshimori
authors.forename: F
authors.initials: F
authors.lastname: Yamagata
authors.forename: A
authors.initials: A
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Mizushima
authors.forename: Y
authors.initials: Y
authors.lastname: Kabeya
authors.forename: A
authors.initials: A
authors.lastname: Nara
authors.forename: I
authors.initials: I
authors.lastname: Miwako
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
authors.forename: M
authors.initials: M
authors.lastname: Ohsumi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohsumi
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001692:Biological Transport; D015415:Biomarkers / Q000032:analysis; D002460:Cell Line; D048429:Cell Size; D011232:Chemical Precipitation; D015536:Down-Regulation; D004705:Endocytosis; D056827:Endosomal Sorting Complexes Required for Transport; D011992:Endosomes / Q000378:metabolism* / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D014168:Transferrin / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases; D014617:Vacuoles / Q000378:metabolism / Q000648:ultrastructure; D033921:Vesicular Transport Proteins
chemical_list: D015415:Biomarkers; D056827:Endosomal Sorting Complexes Required for Transport; D005656:Fungal Proteins; D008077:Lipoproteins, LDL; D011990:Receptors, Transferrin; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014168:Transferrin; C107528:VPS4 protein, S cerevisiae; D033921:Vesicular Transport Proteins; D004815:Epidermal Growth Factor; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases; D000074183:ATPases Associated with Diverse Cellular Activities; C490305:VPS4A protein, human; C089217:Vps4b protein, mouse