Molecular characterization of ubiquitin genes from Aspergillus nidulans: mRNA expression on different stress and growth conditions.
We are interested in studying the ubiquitin (UBI) gene expression during different stress and growth conditions in the filamentous fungus Aspergillus nidulans. Here, we report the cloning of a cDNA clone that corresponds to a gene, ubi1, that encodes a carboxyl extension protein from A. nidulans. This cDNA corresponds to a gene that encodes a protein that showed high homology to other polyubiquitin and CEP-80 genes at the N- and C-terminus, respectively. We characterize the mRNA expression of the CEP and polyubiquitin genes during several growth and stress conditions. Expression of the ubi1 and ubi4 genes was correlated with cell growth in most of the carbon sources used, except maltose. Both ubi1 and ubi4 genes were induced upon heat-shock, although the levels of expression were raised quicker for ubi4 than for ubi1. The ubi1 and ubi4 genes displayed a very complex expression pattern in presence of drugs with a different mechanism of action suggesting that the regulatory processes controlling UBI gene expression discriminate between different stresses and can affect individually each UBI gene. The ubi1 gene was highly expressed in presence of hydrogen peroxide while the ubi4 mRNA level was not affected; several metals in our experimental conditions were not able to induce either ubi1 nor ubi4 genes.
Noventa-Jordão
M A
MA
Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, Universidade de Franca, São Paulo, Brazil.
do Nascimento
A M
AM
Goldman
M H
MH
Terenzi
H F
HF
Goldman
G H
GH
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001704:Biopolymers / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006358:Hot Temperature; D008969:Molecular Sequence Data; D025821:Polyubiquitin; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics*
D001704:Biopolymers; D018076:DNA, Complementary; D011498:Protein Precursors; D012333:RNA, Messenger; D014452:Ubiquitins; C045677:ubiquitin precursor; D025821:Polyubiquitin