title: Interaction of lactoferrin with ceruloplasmin.
abstract: When added to human blood serum, the iron-binding protein lactoferrin (LF) purified from breast milk interacts with ceruloplasmin (CP), a copper-containing oxidase. Selective binding of LF to CP is evidenced by the results of polyacrylamide gel electrophoresis, immunodiffusion, gel filtration, and affinity chromatography. The molar stoichiometry of CP:LF in the complex is 1:2. Near-uv circular dichroism spectra of the complex showed that neither of the two proteins undergoes major structural perturbations when interacting with its counterpart. K(d) for the CP/LF complex was estimated from Scatchard plot as 1.8 x 10(-6) M. The CP/LF complex is found in various fluids of the human body. Upon injection into rat of human LF, the latter is soon revealed within the CP/LF complex of the blood plasma, from where the human protein is substantially cleared within 5 h.
authors.affiliation: Institute for Experimental Medicine, St. Petersburg, Russia.
authors.forename: E T
authors.initials: ET
authors.lastname: Zakharova
authors.forename: M M
authors.initials: MM
authors.lastname: Shavlovski
authors.forename: M G
authors.initials: MG
authors.lastname: Bass
authors.forename: A A
authors.initials: AA
authors.lastname: Gridasova
authors.forename: M O
authors.initials: MO
authors.lastname: Pulina
authors.forename: V
authors.initials: V
authors.lastname: De Filippis
authors.forename: M
authors.initials: M
authors.lastname: Beltramini
authors.forename: P
authors.initials: P
authors.lastname: Di Muro
authors.forename: B
authors.initials: B
authors.lastname: Salvato
authors.forename: A
authors.initials: A
authors.lastname: Fontana
authors.forename: V B
authors.initials: VB
authors.lastname: Vasilyev
authors.forename: V S
authors.initials: VS
authors.lastname: Gaitskhoki
mesh_terms: D000818:Animals; D001059:Apoproteins / Q000737:chemistry; D002570:Ceruloplasmin / Q000737:chemistry* / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007700:Kinetics; D007781:Lactoferrin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008895:Milk, Human / Q000737:chemistry; D051381:Rats
chemical_list: D001059:Apoproteins; D002570:Ceruloplasmin; D007781:Lactoferrin