Identification of matrix metalloproteinases in canine neoplastic tissue.
OBJECTIVE
To identify matrix metalloproteinases (MMP) 2 and 9 in canine tumor tissue and to compare the amount of activity to that in unaffected stromal tissue.


METHODS
30 dogs with spontaneously developing, high-grade osteosarcoma.


METHODS
Tumor and nearby stromal tissue (muscle) were obtained at the time of surgery. Specimens were homogenized, and supernatants were assayed, using gelatin zymography. Human derived standards were run concurrently. Densitometry was done to obtain a semiquantitative arbitrary unit value for each specimen. The amount of activity in tumor tissue was compared with the amount in stromal tissue.


RESULTS
Gelatinolytic bands were observed from the analysis of all tumor tissues and in most stromal tissues. These bands migrated in the same molecular weight area as the human MMP 2 and 9 standards. Gelatinolytic activity could be quenched by the addition of 50 mM EDTA and 1 microg of synthetic tissue inhibitor of metalloproteinase (TIMP) 2 per 100 ml. There was significantly more gelatinolytic activity in tumor tissue than in stromal tissue.


CONCLUSIONS
MMP 2 and 9 are detectable in canine neoplastic tissue. Matrix metalloproteinases activity in tumor tissue is higher than in unaffected stromal tissue, indicating that canine MMP may be involved in the pathogenesis of tumor growth and metastasis.
Lana
S E
SE
Department of Clinical Sciences, College of Veterinary Medicine and Biological Sciences, Colorado State University, Ft. Collins 80523, USA.
Ogilvie
G K
GK
Hansen
R A
RA
Powers
B E
BE
Dernell
W S
WS
Withrow
S J
SJ
D000818:Animals; D001859:Bone Neoplasms / Q000201:enzymology / Q000601:surgery / Q000662:veterinary*; D004283:Dog Diseases / Q000201:enzymology* / Q000601:surgery; D004285:Dogs; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D012516:Osteosarcoma / Q000201:enzymology / Q000601:surgery / Q000662:veterinary*; D017154:Stromal Cells / Q000201:enzymology
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9