Branchiomotor activities in mouse embryo.
Using a novel isolated hindbrain in vitro preparation, we demonstrate that, in the mouse, branchiomotor activities from trigeminal, facial, glossopharyngeal and vagal nerves start during segmentation, a crucial and conserved period of hindbrain embryogenesis. At embryonic day (E) 10.5, branchiomotor nerves are independently active in bursts, become coactive at a low frequency (about 0.5 min-1) at E12.5, before high frequency (about 15 min-1) fetal breathing starts at E14.5. Comparison with observations in chick reveals a transient episodic rhythmic pattern highly similar in mouse at E13.5 and chick at E7. This pattern is proposed as a marker identifying a phylotypic stage during the development of hindbrain neuronal networks in vertebrates.
Abadie
V
V
Institut Alfred Fessard, CNRS, Gif Sur Yvette, France.
Champagnat
J
J
Fortin
G
G
D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000196:embryology; D002642:Chick Embryo; D003391:Cranial Nerves / Q000196:embryology / Q000502:physiology; D004594:Electrophysiology; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D051379:Mice; D009043:Motor Activity / Q000502:physiology*; D009415:Nerve Net / Q000196:embryology; D009474:Neurons / Q000502:physiology; D010641:Phenotype; D011247:Pregnancy; D012249:Rhombencephalon / Q000196:embryology*