Expression of an array of photoreceptor genes in chick embryonic retinal pigment epithelium cell cultures under the induction of neuroD.
Coaxing plastic, non-neuronal cells to transdifferentiate into a particular type of neurons might have clinical applications. Previously we reported that neuroD induces transdifferentiation of retinal pigment epithelium (RPE) cells derived from day-6 chick embryos into cells that resemble young photoreceptor cells. These cells also express visinin, a gene expressed early during cone photoreceptor differentiation. Further characterization showed that the transdifferentiated cells express a number of photoreceptor genes, including interphotoreceptor retinoid binding protein, the alpha-subunit of phosphodiesterase, and opsin genes encoding rhodopsin, the red, the green, and the blue visual pigments. Our data demonstrate that neuroD can reprogram RPE to become photoreceptor cells with substantial differentiation, and suggest the possibility of generating photoreceptor cells from RPE using neuroD as a molecular trigger.
Yan
R T
RT
Department of Ophthalmology, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35233, USA.
Wang
S Z
SZ
D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D005136:Eye Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology; D012177:Retinol-Binding Proteins / Q000235:genetics; D012243:Rhodopsin / Q000235:genetics; D000099214:Retinol-Binding Proteins, Interstitial
D051792:Basic Helix-Loop-Helix Proteins; D005136:Eye Proteins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D010727:Phosphoric Diester Hydrolases; D011993:Recombinant Fusion Proteins; D012177:Retinol-Binding Proteins; D012243:Rhodopsin; D000099214:Retinol-Binding Proteins, Interstitial; C045140:visinin; C093564:Neurogenic differentiation factor 1