Use of linear and multiple antigenic peptides in the immunodiagnosis of acute hepatitis A virus infection.
The reactivities of two panels of anti-HAV human sera from geographically distinct areas (Chile and Spain) to synthetic peptides from the VP1, VP2 and VP3 hepatitis A virus capsid proteins were examined by an enzyme-linked immunosorbent assay (ELISA) procedure. Two and four branched multiple antigenic peptides (MAPs) and palmitoylated peptides were compared with free synthetic sequences for the detection of IgM anti-HAV antibodies in the two panels of human sera. Our results showed that acute hepatitis A patient sera recognized preferentially homogeneous two branched MAPs and palmitic acid conjugated peptides. The palmitoyl-derived VP3(110-121) peptide and the corresponding dimeric MAP were the most sensitive and appropriate for serological studies of HAV-infected patients by ELISA, sensitivity and specificity being higher than 90% and 95%, respectively. These peptide-based tests open up new avenues in the development of peptide-based immunosorbent assays for the detection of acute HAV disease.
Gómara
M J
MJ
Departament de Química de Pèptids i Proteïnes, IIQAB-CSIC, Jordi Girona 18-26, 08034, Barcelona, Spain.
Riedemann
S
S
Vega
I
I
Ibarra
H
H
Ercilla
G
G
Haro
I
I
D000208:Acute Disease; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000138:chemical synthesis / Q000276:immunology*; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006506:Hepatitis A / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D035922:Hepatitis A Antibodies; D035921:Hepatitis A Antigens; D006508:Hepatitis Antibodies / Q000097:blood / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D019308:Palmitic Acid / Q000276:immunology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015678:Viral Structural Proteins / Q000138:chemical synthesis / Q000276:immunology*
D000956:Antigens, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D035922:Hepatitis A Antibodies; D035921:Hepatitis A Antigens; D006508:Hepatitis Antibodies; D010446:Peptide Fragments; D010455:Peptides; D011933:Reagent Kits, Diagnostic; C096539:VP1 protein, hepatitis A virus; C109000:VP3 peptide (110-121), hepatitis A virus; D015678:Viral Structural Proteins; D019308:Palmitic Acid