A rev-like NES mediates cytoplasmic localization of HERV-K cORF.
The human endogenous retrovirus K (HERV-K)-encoded protein cORF has recently been shown to be a functional homolog of the HIV Rev protein. Rev-mediated RNA export requires interaction between a leucine-rich nuclear export signal (NES) in Rev and the nuclear export receptor Crm1/exportin1. Like Rev, cORF binds to Crm1 and cORF-mediated RNA export depends on Crm1 activity. Here we document that mutation of the putative NES in cORF results in trapping of the protein in the nucleus, suggesting that the cORF NES functions in analogy to the Rev NES.
Boese
A
A
Institut für Mikrobiologie und Hygiene, Abteilung Virologie, Haus 47, Universitätskliniken, D-66421, Homburg/Saar, Germany.
Sauter
M
M
Mueller-Lantzsch
N
N
D020816:Amino Acid Motifs / Q000235:genetics; D001692:Biological Transport / Q000235:genetics; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology*; D020077:Endogenous Retroviruses / Q000235:genetics* / Q000378:metabolism*; D015694:Gene Products, rev / Q000235:genetics*; D049452:Green Fluorescent Proteins; D006801:Humans; D007150:Immunohistochemistry; D007202:Indicators and Reagents; D028884:Karyopherins; D008164:Luminescent Proteins; D016297:Mutagenesis, Site-Directed; D017434:Protein Structure, Tertiary / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D000097606:Exportin 1 Protein
D002352:Carrier Proteins; D015694:Gene Products, rev; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents; D028884:Karyopherins; D008164:Luminescent Proteins; D012367:RNA, Viral; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D000097606:Exportin 1 Protein; C406149:HERV-K cORF protein, Human endogenous retrovirus K