Determination of diclofenac sodium by capillary zone electrophoresis with electrochemical detection.
Capillary zone electrophoresis was employed for the determination of diclofenac sodium using an end-column amperometric detection with a carbon fiber microelectrode, at a constant potential of 0.83 V vs. saturated calomel electrode. The optimum conditions of separation and detection are 4.90 x 10(-3) mol/l Na2HPO4-3.10 x 10(-3) mol/l NaH2PO4 (pH 7.0) for the buffer solution, 10 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 2.5 x 10(-6) mol/l or 5.2 fmol (S/N=2). The relative standard deviation is 0.8% for the migration time and 4.7% for the electrophoretic peak current. The method was applied to the determination of diclofenac sodium in human urine.
Ji
W
W
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China. wenruijin@jn-public.sd.cninfo.net
Zhan
J
J
D004008:Diclofenac / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011786:Quality Control; D015203:Reproducibility of Results
D004008:Diclofenac