The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells.
The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.
Odum
N
N
Institute of Medical Microbiology and Immunology, The Panum Institute, Copenhagen, Denmark.
Bregenholt
S
S
Eriksen
K W
KW
Skov
S
S
Ryder
L P
LP
Bendtzen
K
K
Van Neerven
R J
RJ
Svejgaard
A
A
Garred
P
P
D015415:Biomarkers; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D019713:Receptors, CCR5 / Q000235:genetics* / Q000276:immunology*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology*
D015415:Biomarkers; D019713:Receptors, CCR5