Detection of viral ribonucleic acid and histologic analysis of inflamed synovium in Ross River virus infection.
OBJECTIVE
To document the histology of Ross River virus (RRV) arthritis and to examine inflamed synovium for viral RNA.


METHODS
Biopsy tissue from the inflamed knees of 12 patients with RRV infection was studied using conventional and immunostaining techniques. Reverse transcriptase-polymerase chain reaction technology was used to probe for the presence of viral RNA in the synovial biopsy samples and in serum.


RESULTS
Hyperplasia of the synovial lining layer, vascular proliferation, and mononuclear cell infiltration were the main histologic changes. RRV RNA was found in knee biopsy tissue that was obtained from 2 patients at 5 weeks after the onset of symptoms.


CONCLUSIONS
RRV RNA was identified in inflamed synovium more than a month after symptoms began. Inflammation was apparent in the absence of detectable virus in the majority of patients.
Soden
M
M
University of Queensland, Townsville, Australia.
Vasudevan
H
H
Roberts
B
B
Coelen
R
R
Hamlin
G
G
Vasudevan
S
S
La Brooy
J
J
D000328:Adult; D018354:Alphavirus Infections / Q000473:pathology*; D001706:Biopsy; D016516:CD4-CD8 Ratio; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012398:Ross River virus / Q000235:genetics; D013583:Synovial Membrane / Q000473:pathology / Q000821:virology*; D013997:Time Factors
D012367:RNA, Viral