title: Arabidopsis cytochrome P450s that catalyze the first step of tryptophan-dependent indole-3-acetic acid biosynthesis.
abstract: Plants synthesize numerous secondary metabolites that are used as developmental signals or as defense against pathogens. Tryptophan (Trp)-derived secondary metabolites include camalexin, indole glucosinolates, and indole-3-acetic acid (IAA); however, the steps in their synthesis from Trp or its precursors remain unclear. We have identified two Arabidopsis cytochrome P450s (CYP79B2 and CYP79B3) that can convert Trp to indole-3-acetaldoxime (IAOx), a precursor to IAA and indole glucosinolates.
authors.affiliation: Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Hull
authors.forename: R
authors.initials: R
authors.lastname: Vij
authors.forename: J L
authors.initials: JL
authors.lastname: Celenza
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010091:Oximes / Q000378:metabolism; D030821:Plants, Genetically Modified; D011549:Pseudomonas / Q000502:physiology; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010091:Oximes; C030737:indoleacetic acid; D014364:Tryptophan; D003577:Cytochrome P-450 Enzyme System; C530433:cytochrome P-450 79B3, Arabidopsis; C023728:acetaldehyde oxime