[Expression of telomerase in acute leukemia].
To understand the expression patterns of telomerase activity in different types of acute leukemia(AL) and during remission state, the mono-nuclear cells of different bone marrow samples were isolated by desity gradient centrifugation. Telomerase activity of the samples was assayed by telomeric repeat amplification protocol(TRAP) with the cell extracts and the TRAP products were resolved in PAGE. The results showed that the telomerase activity was at higher levels in the acute leukemia compared with the normal controls. Of the acute nonlymphocytic leukemia(ANLL), the telomerase activity presented as M2a > M5b > M1 > M3b. Of the acute lymphocytic leukemia(ALL), the activity presented as L1 > L2. The activity assayed in M2a and L2 remission was as high as that in the normal group. It indicated that the expression of telomerase was enhanced differently among the subtypes of ANLL and ALL, and was down-regulated during the AL remission. It implicates that the expression patterns of telomerase activity in AL is associated with the different proliferation states and differentiation properties of the AL cells.
Peng
J
J
Department of Clinical Biochemistry, Hunan Medical University, Changsha.
Chen
Z
Z
Li
Z
Z
Luo
J
J
Wu
Y
Y
Liu
L
L
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000201:enzymology; D002648:Child; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology*; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology*; D019098:Telomerase / Q000378:metabolism*
D019098:Telomerase