Direct interaction between emerin and lamin A.
Emerin is the protein of the inner nuclear membrane that is affected by mutation in X-linked Emery-Dreifuss muscular dystrophy. The autosomal dominant form of the disease is caused by mutations in the lamin A/C gene. Several lines of circumstantial evidence have suggested an interaction of emerin with lamins in the nuclear lamina but direct interaction between the two proteins has not yet been demonstrated. We now demonstrate direct interaction between recombinant emerin and lamin A molecules using biomolecular interaction analysis (BIA) and monoclonal antibodies. An emerin-lamin A interaction system may be related in function to the LAP2-lamin B system at the inner nuclear rim.
Clements
L
L
MRIC Biochemistry Group, N.E. Wales Institute, Wrexham, LL11 2AW, United Kingdom.
Manilal
S
S
Love
D R
DR
Morris
G E
GE
D000911:Antibodies, Monoclonal; D001665:Binding Sites; D003001:Cloning, Molecular; D006801:Humans; D034904:Lamin Type A; D034882:Lamins; D008565:Membrane Proteins / Q000737:chemistry*; D020389:Muscular Dystrophy, Emery-Dreifuss; D009687:Nuclear Proteins / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013946:Thymopoietins / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D034904:Lamin Type A; D034882:Lamins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D013946:Thymopoietins; C092710:emerin