title: Structural conservation and variation among U5 small nuclear RNAs from trypanosomatid protozoa.
abstract: U5 snRNAs in trypanosomatid protozoa do not contain the trimethylguanosine cap structures that are often targeted in snRNA isolation procedures. As a result, the trypanosomatids are not well represented in the database of available U5 snRNA sequences. We have isolated and determined the sequence of the U5 snRNA from Crithidia fasciculata. Comparison with previously published trypanosomatid U5 snRNA sequences allows us to deduce the pattern of structural conservation and variation among these very divergent snRNA molecules.
authors.affiliation: Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada.
authors.forename: M N
authors.initials: MN
authors.lastname: Schnare
authors.forename: M W
authors.initials: MW
authors.lastname: Gray
mesh_terms: D000818:Animals; D001483:Base Sequence; D016831:Crithidia fasciculata / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006151:Guanosine / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D012313:RNA / Q000737:chemistry; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014345:Trypanosoma / Q000235:genetics*
chemical_list: D012342:RNA, Small Nuclear; D006151:Guanosine; D012313:RNA