Novel approaches to monitor bacterial gene expression in infected tissue and host.
Elucidating the complex and dynamic host-microbe interactions during infection requires, among other things, detailed knowledge of microbial gene expression in vivo. Recently, advances in fluorescence and bioluminescence detection techniques, as well as recombinase-based in vivo expression technology, have rendered monitoring virulence gene expression in vivo a feasible task. These techniques have been adapted by several laboratories to study the spatial and temporal patterns of virulence gene expression by organisms such as Salmonella typhimurium, Listeria monocytogenes, Yersinia entercolitica and Vibrio cholerae during infection of tissue culture or animal models of infection.
Lee
S H
SH
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
Camilli
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D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D001424:Bacterial Infections / Q000382:microbiology*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D019426:Integrases; D008156:Luciferases / Q000378:metabolism; D008164:Luminescent Proteins; D051379:Mice; D045522:Recombinases; D014774:Virulence / Q000235:genetics
D008164:Luminescent Proteins; D045522:Recombinases; D049452:Green Fluorescent Proteins; D008156:Luciferases; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C115006:integron integrase IntI1