title: Regression of experimental liver tumor after distant intra-hepatic injection of cytosine deaminase-expressing tumor cells and 5-fluorocytosine treatment.
abstract: Cytosine deaminase (CD) gene of E. coli converts the non-toxic compound 5-fluorocytosine (5-FC) into 5-fluorouracil. We have introduced a vector expressing the CD gene in a rat colon carcinoma cell line. Expression of the CD gene confers 5-FC sensitivity to these cells in vitro and in vivo. In a bifocal model consisting in a simultaneous engrafment of a CD+ tumor on one lobe of the liver and a wild-type parental tumor on the opposite lobe, treatment with 5-FC results in regression of both type of tumors, indicating the existence of a distant bystander effect.
authors.affiliation: Unite INSERM 364, Faculte de Medecine, 06107 Nice, Cedex 2, France.
authors.forename: V
authors.initials: V
authors.lastname: Pierrefite-Carle
authors.forename: P
authors.initials: P
authors.lastname: Baque
authors.forename: A
authors.initials: A
authors.lastname: Gavelli
authors.forename: D
authors.initials: D
authors.lastname: Benchimol
authors.forename: A
authors.initials: A
authors.lastname: Bourgeon
authors.forename: G
authors.initials: G
authors.lastname: Milano
authors.forename: M C
authors.initials: MC
authors.lastname: Saint-Paul
authors.forename: B
authors.initials: B
authors.lastname: Rossi
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology; D043525:Cytosine Deaminase; D005437:Flucytosine / Q000494:pharmacology*; D015316:Genetic Therapy; D007267:Injections; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D008297:Male; D009368:Neoplasm Transplantation; D009700:Nucleoside Deaminases / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D005437:Flucytosine; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase