Gas chromatographic quantification of amino acid enantiomers in food matrices by their N(O,S)-ethoxycarbonyl heptafluorobutyl ester derivatives.
Several amino acid enantiomer derivatives were prepared with different chloroformates and analysed by gas chromatography (GC) on a Chirasil-L-Val GC column, at a temperature below 200 degrees C. Among them the N(O,S)-ethoxycarbonyl heptafluorobutyl esters presented the best compromise between short retention times, high yield responses and good resolution for almost all the tested amino acids. These derivatives proved to be suited for quantification of amino acids in aqueous media, with L-p-chlorophenylalanine as internal standard. The developed procedure was applied to several food samples for determination of their free amino acid profiles.
Casal
S
S
CEQUP/Serviço de Bromatologia, Faculdade de Farmácia da Universidade do Porto, Portugal.
Oliveira
M B
MB
Ferreira
M A
MA
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D004952:Esters / Q000737:chemistry*; D005466:Fluorocarbons / Q000737:chemistry*; D005504:Food Analysis; D013237:Stereoisomerism
D000596:Amino Acids; D004952:Esters; D005466:Fluorocarbons; C404445:N(O,S)-ethoxycarbonyl heptafluorobutyl ester