[PCR-SSCP analysis of p53 gene in human primary brain tumor].
In this study PCR-SSCP alalysis was made to detect the mutation of the exon 5-7 of p53 gene in 37 cases of human primary brain tumor. The results showed that 10 human primary brain tumors had mutation, of which 9 were gliomas (37.5%) and 1 was meningioma (7.7%), and that the higher the grade of tumor was, the higher would be the frequency of mutation. These results suggest that human primary brain tumor is related to p53 gene mutation and such mutation may be the major reason for human primary brain tumor genesis and malignancy.
Tan
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Department of Biochemistry, School of Basic Medical Sciences, Chengdu.
Sun
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D001254:Astrocytoma / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics*; D005091:Exons; D016158:Genes, p53; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics; D008579:Meningioma / Q000235:genetics; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational