Analysis of oligodendroglial differentiation using cDNA arrays.
We used cDNA arrays to investigate molecular aspects of the differentiation of an immortalized line of oligodendroglial progenitors, and of immunopan-purified primary cultures of oligodendroglial progenitors, to immature oligodendroglia. Developmental regulation of the proteolipid and 2-hydroxyacylsphingosine 1-galactosyltransferase genes was tighter in the primary than in the immortalized cells. Our data suggest that increased expression of genes encoding the following proteins are involved in oligodendroglial differentiation: Fyn, Erk, p85, G-alpha-12 guanine nucleotide binding, and transducin beta-2 signal transduction molecules; glial maturation factor; the proteasomal subunits C8 and C3; the proteasomal targeting molecule polyubiquitin; the cell cycle regulatory proteins Set, protein phosphatase 2A, and nuclear tyrosine phosphatase (PRL-1); and the high-affinity glutamate cotransporter EAAC-1.
Scarlato
M
M
Division of Neurology, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.
Beesley
J
J
Pleasure
D
D
D000293:Adolescent; D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology; D002461:Cell Line, Transformed; D016922:Cellular Senescence / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D009419:Nerve Tissue Proteins / Q000235:genetics; D009836:Oligodendroglia / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015854:Up-Regulation
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger