[Effects of mifepristone on proliferation and apoptosis in early chorionic villi].
OBJECTIVE
To study the effects of mifepristone on proliferation and apoptosis in early pregnant chorionic villi.


METHODS
Proliferation and apoptosis in early pregnant choionic villi from surgical aspiration and mifepristone induced abortion were studied. Marker for proliferation is proliferating cell nuclear antigen (PCNA) immunohistochemical staining, apoptotic cell death was detected using DNA in situ terminal deoxynucleotidyl transferas-mediated dUTP-biotin nick ending labeling (TUNEL).


RESULTS
In early pregnant chorionic villi, proliferating index of cytotrophoblasts was 53.74% +/- 1.34%, not only nuclear but also cytoplasma were PCNA positive staining, syncytiotrophoblasts were negative. There existed apoptotic cell death in trophoblasts, the apoptotic index were 2.52% +/- 0.86% in syncytiotrophoblasts and 0.52% +/- 0.26% in cytotrophblasts, respectively. After 2 days mifepristone treatment, the proliferating index of cytotrophoblasts decreased slightly and PCNA positive cytoplasmic expression disappeared; while apoptotic cell increased significantly, the apoptotic indices were 22.16% +/- 2.26% in syncytiotrophoblasts and 20.12% +/- 1.74% in cytotrophoblasts, respectively.


CONCLUSIONS
Mifepristone may inhibit proliferation and promote apoptosis of trophoblasts in early pregnant chorionic villi.
Yang
Y
Y
Si Chuan Provincial Hospital, ChengDu.
Cao
Z
Z
Han
Z
Z
D000021:Abortifacient Agents, Steroidal / Q000494:pharmacology*; D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002824:Chorionic Villi / Q000187:drug effects; D005260:Female; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
D000021:Abortifacient Agents, Steroidal; D018809:Proliferating Cell Nuclear Antigen; D015735:Mifepristone