Coexistence of several unbalanced translocations in a case of neuroblastoma: the contribution of multicolor spectral karyotyping.
Spectral karyotyping (SKY) is based on the simultaneous hybridization of a set of 24 chromosome-specific DNA painting probes, each labeled with a different fluor combination. Automatic classification, based on the measurement of the spectrum for each chromosome, was applied to metaphases obtained from the affected bone marrow of a neuroblastoma case. Spectral karyotyping allowed the identification of chromosomal aberrations that could not be identified by the use of the G-banding technique, and revealed a number of gains and unbalanced translocations.
Trakhtenbrot
L
L
Institute of Hematology, Chaim Sheba Medical Center, Tel Hashomer, Israel.
Cohen
N
N
Rosner
E
E
Gipsh
N
N
Brok-Simoni
F
F
Mandel
M
M
Amariglio
N
N
Rechavi
G
G
D002675:Child, Preschool; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping / Q000379:methods*; D009447:Neuroblastoma / Q000235:genetics*; D014178:Translocation, Genetic