title: Naked DNA vaccination of Atlantic salmon Salmo salar against IHNV.
abstract: A naked plasmid DNA encoding the glycoprotein (pCMV4-G) of a 1976 isolate of infectious hematopoietic necrosis virus (IHNV) obtained from steelhead Oncorhynchus mykiss was used to vaccinate Atlantic salmon Salmo salar against IHNV. Eight weeks post-vaccination the fish were challenged with a strain of IHNV originally isolated from farmed Atlantic salmon undergoing an epizootic. Fish injected with the glycoprotein-encoding plasmid were significantly (p < 0.05) protected against IHNV by both immersion and cohabitation challenge. Survivors of the first challenges were pooled and re-challenged by immersion 12 wk after the initial challenge. Significant (p < 0.05) protection was observed in all of the previously challenged groups including those receiving the complete vaccine. Fish injected with the glycoprotein-encoding plasmid produced low levels of virus-neutralizing antibodies prior to the first challenge. Neutralizing antibodies increased in all groups after exposure to the IHNV. Passive transfer of pooled sera from pCMV4-G vaccinates and IHN survivors provided relative survivals of 40 to 100% compared to fish injected with sera collected from fish immunized with control vaccines or left unhandled. In this study, DNA vaccination effectively protected Atlantic salmon smolts against challenges with IHNV.
authors.affiliation: Department of Fisheries and Oceans, Pacific Biological Station, Nanaimo, British Columbia, Canada. traxlerg@dfo-mpo.gc.ca
authors.forename: G S
authors.initials: GS
authors.lastname: Traxler
authors.forename: E
authors.initials: E
authors.lastname: Anderson
authors.forename: S E
authors.initials: SE
authors.lastname: LaPatra
authors.forename: J
authors.initials: J
authors.lastname: Richard
authors.forename: B
authors.initials: B
authors.lastname: Shewmaker
authors.forename: G
authors.initials: G
authors.lastname: Kurath
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017756:Aquaculture; D004279:DNA, Viral / Q000737:chemistry; D018562:Disease Transmission, Infectious / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D005618:Fresh Water; D007101:Immersion; D007116:Immunization, Passive / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D017686:Oncorhynchus mykiss / Q000821:virology; D010957:Plasmids / Q000737:chemistry; D012209:Rhabdoviridae / Q000235:genetics / Q000276:immunology*; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D020317:Salmo salar; D012623:Seawater; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology / Q000592:standards; D014765:Viral Vaccines / Q000276:immunology* / Q000592:standards
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D019444:Vaccines, DNA; D014765:Viral Vaccines