title: Arterial to inspired partial pressure ratio of halothane, isoflurane, sevoflurane and desflurane in rats.
abstract: The inspired partial pressure of an anaesthetic is often used as an index of arterial partial pressure in small animal experiments. We have investigated the influence of anaesthetic solubility on the ratio of arterial to inspired partial pressure in 24 rats, allocated randomly to receive halothane, isoflurane or desflurane at four different inspired concentrations. The arterial partial pressure of the volatile agent was measured by two-stage headspace analysis using a gas chromatograph calibrated with the same gas used to calibrate the Datex Capnomac that measured the inspired concentration. Mean values of arterial to inspired ratio at the lowest concentrations were 0.60 (95% confidence intervals 0.50, 0.71) for 0.8% halothane, 0.54 (0.38, 0.69) for 0.8% isoflurane, 0.72 (0.59, 0.86) for 1.5% sevoflurane and 0.71 (0.54, 0.87) for 4% desflurane. Analysis of variance showed a significant effect of anaesthetic agent (P = 0.008) on the arterial to inspired ratio. Thus volatile anaesthetic agents do not demonstrate a fixed arterial to inspired ratio in rats.
authors.affiliation: Department of Anaesthesia, Imperial College School of Medicine, Hammersmith Hospital, London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Holdcroft
authors.forename: D
authors.initials: D
authors.lastname: Bose
authors.forename: S M
authors.initials: SM
authors.lastname: Sapsed-Byrne
authors.forename: D
authors.initials: D
authors.lastname: Ma
authors.forename: G G
authors.initials: GG
authors.lastname: Lockwood
mesh_terms: D018685:Anesthetics, Inhalation / Q000008:administration & dosage / Q000097:blood*; D000818:Animals; D002245:Carbon Dioxide / Q000097:blood; D000077335:Desflurane; D004305:Dose-Response Relationship, Drug; D005260:Female; D006221:Halothane / Q000008:administration & dosage / Q000097:blood; D001239:Inhalation; D007530:Isoflurane / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood; D008738:Methyl Ethers / Q000008:administration & dosage / Q000097:blood; D010313:Partial Pressure; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D002245:Carbon Dioxide; D000077149:Sevoflurane; D000077335:Desflurane; D007530:Isoflurane; D006221:Halothane