title: Detection of single-stranded cohesive ends in the genome of Bacillus thuringiensis temperate phage KK-88.
abstract: Cohesive ends (cos sites) were detected in the genome of temperate KK-88 phage in Bacillus thuringiensis after analysis of the phage DNA generated by the 3'-5' exonuclease activity of Klenow fragment of DNA polymerase I. In addition, unlike the 5'-protruding ends of coliphage lambda genome, the ends of KK-88 phage genome were found to be 3'-protruding. The restriction map of the phage genome was also constructed on the basis of position of the cos fragments.
authors.affiliation: Department of Applied Biological Sciences, Saga University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kanda
authors.forename: M
authors.initials: M
authors.lastname: Tachi
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: F
authors.initials: F
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Murata
mesh_terms: D001413:Bacillus thuringiensis / Q000821:virology*; D001435:Bacteriophages / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D016679:Genome, Viral; D015183:Restriction Mapping
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral