title: Study of the virulence of five strains of amyxomatous myxoma virus in crossbred New Zealand White/Californian conventional rabbits, with evidence of long-term testicular infection in recovered animals.
abstract: The virulence of five amyxomatous myxoma virus (MV) strains, the clinical and pathogenetic effects of which had been studied previously in specific pathogen-free (SPF) rabbits, was determined by inoculation of five groups of 10 crossbred New Zealand White/Californian conventional rabbits. A much more acute myxomatosis syndrome was produced in conventional rabbits than that reproduced previously in SPF animals. However, the main clinical signs were of the respiratory type. The MV strains MYX 254/95 and 801 appeared very virulent, killing all the inoculated animals. The strains MYX 217/95, MYX 555/94 and Saint Benoist were somewhat attenuated, killing only seven, six and six rabbits, respectively. Extensive lung lesions due to supervening bacterial infections were observed in 36 of the 39 rabbits that died. Lethality was found to be a better estimate of virulence than mean survival time. By 98 days after viral inoculation, all the surviving animals had completely recovered. At that time, they were immunosuppressed by treatment with adrenocorticotrophic hormone (ACTH) for 10 days to determine whether they still harboured the virus. After the ACTH treatment, eight of the 11 surviving rabbits showed clinical signs that resembled amyxomatous myxomatosis. All the virological examinations performed on naso-conjunctival exudate, on mononuclear cells, on eyelids and on ovaries remained negative but infectious virus was isolated from the testes of three of six surviving male rabbits.
authors.affiliation: Department of Bird and Rabbit Medicine, Faculty of Veterinary Medicine, University of Liège, Bld de Colonster 20,Bat B42, Sart-Tilman, Liège, B4000, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Marlier
authors.forename: J
authors.initials: J
authors.lastname: Mainil
authors.forename: J
authors.initials: J
authors.lastname: Sulon
authors.forename: J F
authors.initials: JF
authors.lastname: Beckers
authors.forename: A
authors.initials: A
authors.lastname: Linden
authors.forename: H
authors.initials: H
authors.lastname: Vindevogel
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000627:therapeutic use; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000187:drug effects; D001947:Breeding; D003433:Crosses, Genetic; D005260:Female; D006854:Hydrocortisone / Q000097:blood; D008168:Lung / Q000382:microbiology / Q000473:pathology / Q000821:virology; D008297:Male; D009233:Myxoma virus / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity*; D009234:Myxomatosis, Infectious / Q000401:mortality / Q000473:pathology* / Q000821:virology; D011817:Rabbits; D013211:Staphylococcus aureus / Q000302:isolation & purification; D015996:Survival Rate; D013737:Testis / Q000821:virology*; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone