Determination of spinosad and its metabolites in citrus crops and orange processed commodities by HPLC with UV detection.
Spinosad is an insect control agent that is derived from a naturally occurring organism and is effective on a wide variety of crops, including citrus crops. A method is described for the determination of spinosad and its metabolites in citrus crops and orange processed commodities. The method determines residues of the active ingredients (spinosyns A and D) and three minor metabolites (spinosyn B, spinosyn K, and N-demethylspinosyn D). For dried orange pulp and orange oil, the method has a limit of quantitation (LOQ) of 0.02 microg/g and a limit of detection (LOD) of 0.006 microg/g. For all other sample matrices (whole fruit, edible fruit, juice, and peel), the method has an LOQ of 0.01 microg/g and an LOD of 0.003 microg/g. The analytes are extracted from the various sample types using appropriate solvents, and the extracts are purified by liquid-liquid partitioning and/or solid-phase extraction. All five analytes are determined simultaneously in the purified extracts by reversed-phase high-performance liquid chromatography with ultraviolet detection at 250 nm.
West
S D
SD
Global Environmental Chemistry Laboratory-Indianapolis Lab, Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana 46268-1054, USA. sdwest@dowagro.com
Turner
L G
LG
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002957:Citrus / Q000737:chemistry*; D018942:Macrolides; D008956:Models, Chemical; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D018942:Macrolides; C114883:spinosyn A; C114884:spinosyn D