The distribution of neuronal nitric oxide synthase in the nucleus tractus solitarii of the squirrel monkey.
The distribution of neuronal nitric oxide synthase (nNOS) containing neurons and fibers in subnuclei of the nucleus tractus solitarii (NTS) in the squirrel monkey, Saimuri sciureus, was investigated by nNOS immunohistochemistry and nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry. Generally, the staining pattern of nNOS and NADPH-diaphorase in the NTS was similar. A high density of neurons and fibers exhibiting both nNOS immunoreactivity and NADPH-diaphorase reactivity was present in the central, medial, intermediate, and dorsolateral subnuclei of the NTS. A moderate density of neurons and fibers that stained for both nNOS and NADPH-diaphorase was noted in the interstitial and ventromedial subnuclei. The gelatinosus and commissural subnuclei contained a low density of neurons and fibers exhibiting nNOS immunoreactivity and NADPH-diaphorase staining. The dorsal motor nucleus of vagus contained a high density of nNOS immunopositive and NADPH-diaphorase containing neurons and fibers at the rostral level, but contained a moderate density of positive fibers and very few positive neurons at the intermediate, subpostremal and commissural NTS levels. Incongruence was noted, however, between nNOS immunostaining and NADPH-diaphorase staining in blood vessels in the brainstem. Capillaries and small vessels exhibited strong staining for NADPH-diaphorase but no nNOS immunoreactivity. In summary, this work substantiates the presence of nNOS in subnuclei of the monkey NTS and is consistent with a role for NO(.) in neurotransmission in primate NTS.
Lin
L H
LH
Department of Neurology, University of Iowa, Iowa City, IA, USA.
Sahai
A K
AK
Rockland
K S
KS
Talman
W T
WT
D000818:Animals; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000201:enzymology / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D012453:Saimiri; D017552:Solitary Nucleus / Q000166:cytology / Q000201:enzymology*
D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D008058:Dihydrolipoamide Dehydrogenase