Efficacy of the "baby-sitter" procedure after prolonged denervation.
This study was undertaken to evaluate whether 40 percent of the hypoglossal nerve, which showed optimal efficacy in restoring orbicularis oculi muscle (OOM) function after different percentages of partial neurectomy in a previous study would be effective after prolonged denervation time. Twenty Sprague-Dawley rats were divided into four groups. In first-stage surgery the left facial nerve of all animals was transected at the level of the stylomastoid foramen and main zygomatic branch. Group A (controls) consisted of animals with only left facial nerves transected (no repair). In Groups B, C, and D the facial nerve was transected and the facial musculature was denervated for a period of 4, 8, and 12 weeks respectively. During a second-stage procedure, a 40 percent neurectomy was performed on the hypoglossal nerve. Subsequently, a nerve transfer was performed by coaptations of a saphenous nerve graft to the neurectomized hypoglossal nerve and the main zygomatic branch of the facial nerve that innervated the OOM. Behavioral analysis of blink reflex, electrophysiology, and axon and motor end-plate counts in Groups B, C, and D showed superior results compared to Group A. There was no statistically significant difference observed among Groups B, C, and D (p > 0.05). Despite the diminished number of axons in the zygomatic branch and motor end-plates in the orbicularis oculi muscle after 12 weeks of denervation, there was still sufficient muscle target recovery to effect some eye closure in all groups except the controls. This study demonstrated in this model that the 40 percent partial neurectomy of the XII to VII component of the "baby-sitter" procedure was effective even after prolonged denervation.
Mersa
B
B
Department of Surgery, Eastern Virginia Medical School, Microsurgical Research Center, Norfolk, Virginia 23501, USA.
Tiangco
D A
DA
Terzis
J K
JK
D000818:Animals; D001767:Blinking / Q000502:physiology; D005154:Facial Nerve / Q000601:surgery; D005158:Facial Paralysis / Q000601:surgery; D007002:Hypoglossal Nerve / Q000502:physiology* / Q000601:surgery; D008297:Male; D009121:Muscle Denervation; D016067:Nerve Transfer; D009801:Oculomotor Muscles / Q000294:innervation* / Q000502:physiology / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors