Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction.
The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied in mice lacking these key enzymes. PI3Kgamma was required for chemoattractant-induced production of phosphatidylinositol 3,4,5-trisphosphate [PtdIns (3,4,5)P3] and has an important role in chemoattractant-induced superoxide production and chemotaxis in mouse neutrophils and in production of T cell-independent antigen-specific antibodies composed of the immunoglobulin lambda light chain (TI-IglambdaL). The study of the mice lacking PLC-beta2 and -beta3 revealed that the PLC pathways have an important role in chemoattractant-mediated production of superoxide and regulation of protein kinases, but not chemotaxis. The PLC pathways also appear to inhibit the chemotactic activity induced by certain chemoattractants and to suppress TI-IglambdaL production.
Li
Z
Z
Department of Pharmacology, University of Rochester, Rochester, NY 14642, USA.
Jiang
H
H
Xie
W
W
Zhang
Z
Z
Smrcka
A V
AV
Wu
D
D
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D054407:Chemokine CCL4; D002630:Chemotactic Factors / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis; D007527:Isoenzymes / Q000378:metabolism*; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D010538:Peritonitis / Q000276:immunology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D054799:Phospholipase C beta; D010766:Phosphorylation; D015398:Signal Transduction; D012883:Skin Ulcer / Q000473:pathology; D013481:Superoxides / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
D054407:Chemokine CCL4; D002630:Chemotactic Factors; D007146:Immunoglobulin lambda-Chains; D007527:Isoenzymes; D019402:Macrophage Inflammatory Proteins; D018129:Phosphatidylinositol Phosphates; C060974:phosphatidylinositol 3,4,5-triphosphate; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019869:Phosphatidylinositol 3-Kinases; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518037:Plcb2 protein, mouse; C518040:Plcb3 protein, mouse