Restriction-fragment-length polymorphism analysis of small-subunit rRNA genes of Blastocystis hominis isolates from geographically diverse human hosts.
Genomic diversity among 14 isolates of Blastocystis hominis from 4 different geographic locations was examined by small-subunit rRNA (ssu rRNA) restriction-fragment-length polymorphisms (RFLP) using 5 different restriction endonucleases. On the basis of the observed RFLP patterns among the isolates, a total of 12 genotypes were identified, with 7 isolates exhibiting mixed RFLP genotypes. There was no correlation between B. hominis geographic origin and RFLP banding pattern or genotype.
Hoevers
J
J
College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
Holman
P
P
Logan
K
K
Hommel
M
M
Ashford
R
R
Snowden
K
K
D000818:Animals; D016844:Blastocystis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016776:Blastocystis Infections / Q000469:parasitology; D015252:Deoxyribonucleases, Type II Site-Specific; D017125:Genes, Protozoan; D020459:Genes, rRNA / Q000235:genetics*; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics
D012335:RNA, Ribosomal; D015252:Deoxyribonucleases, Type II Site-Specific