Effects of jasplakinolide on the kinetics of actin polymerization. An explanation for certain in vivo observations.
Jasplakinolide paradoxically stabilizes actin filaments in vitro, but in vivo it can disrupt actin filaments and induce polymerization of monomeric actin into amorphous masses. A detailed analysis of the effects of jasplakinolide on the kinetics of actin polymerization suggests a resolution to this paradox. Jasplakinolide markedly enhances the rate of actin filament nucleation. This increase corresponds to a change in the size of actin oligomer capable of nucleating filament growth from four to approximately three subunits, which is mechanistically consistent with the localization of the jasplakinolide-binding site at an interface of three actin subunits. Because jasplakinolide both decreases the amount of sequestered actin (by lowering the critical concentration of actin) and augments nucleation, the enhancement of polymerization by jasplakinolide is amplified in the presence of actin-monomer sequestering proteins such as thymosin beta(4). Overall, the kinetic parameters in vitro define the mechanism by which jasplakinolide induces polymerization of monomeric actin in vivo. Expected consequences of jasplakinolide function are consistent with the experimental observations and include de novo nucleation resulting in disordered polymeric actin and in insufficient monomeric actin to allow for remodeling of stress fibers.
Bubb
M R
MR
Department of Medicine, University of Florida, Gainesville, Florida 32610, USA.
Spector
I
I
Beyer
B B
BB
Fosen
K M
KM
D000199:Actins / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001704:Biopolymers; D002460:Cell Line; D047630:Depsipeptides; D007700:Kinetics; D010456:Peptides, Cyclic / Q000494:pharmacology; D010590:Phalloidine / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D013947:Thymosin / Q000494:pharmacology; D000099201:Thymosin beta(4)
D000199:Actins; D001704:Biopolymers; D047630:Depsipeptides; D010456:Peptides, Cyclic; D010590:Phalloidine; D013947:Thymosin; D000099201:Thymosin beta(4); C057531:jasplakinolide