title: Vaccination of Manchego lambs against Haemonchus contortus with a somatic fraction (p26/23) of adult parasites.
abstract: A low molecular weight fraction from adult Haemonchus contortus containing two peptides (p26/23) was used to vaccinate Manchego female lambs between 3.5 and 5 months of age. Immunizing injections were given three times on days 0, 14 and 28 of the experiment. On day 43, lambs were challenged with 400 third stage larvae/kg live weight. Vaccination induced a lengthening of prepatent periods, significant reduction (> 60%) in mean faecal egg counts and smaller variations in packed cell volume values. At necropsy, average worm burden in the vaccinated lambs was significantly lower (61.6%) than that found in unvaccinated challenged animals. A clear correlation was found between protection and serum antibody response in immunized lambs.
authors.affiliation: Departamento de Patologia Animal I, Facultad de Veterinaria, Universidad Complutense, E-28040 Madrid, Spain.
authors.forename: I A
authors.initials: IA
authors.lastname: Domínguez-Toraño
authors.forename: M
authors.initials: M
authors.lastname: Cuquerella
authors.forename: M
authors.initials: M
authors.lastname: Gómez-Muñoz
authors.forename: S
authors.initials: S
authors.lastname: Mendez
authors.forename: F J
authors.initials: FJ
authors.lastname: Fernández-Pérez
authors.forename: J M
authors.initials: JM
authors.lastname: Alunda
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000737:chemistry / Q000276:immunology*; D015801:Helminth Proteins / Q000008:administration & dosage / Q000276:immunology; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D010455:Peptides; D014612:Vaccines