title: Activities of the bimodal fluorescent protein produced by Photobacterium phosphoreum strain bmFP in the luciferase reaction in vitro.
abstract: The activity of the bimodal fluorescent protein (bmFP) (lambda max, 488 and 517 nm) in the in vitro luciferase reaction has been studied. The bmFP that is produced by Photobacterium phosphoreum strain bmFP is a dimer of two homologous subunits binding four riboflavin 5'-phosphate (FMN)-myristate chromophores. The addition of bmFP to the luciferase reaction in the presence of the lumazine protein prevented the lumazine protein-induced blue shift in the emission band. The bmFP reduced electrochemically serves as a substrate in the luciferase reaction in the absence of added FMN, resulting in light emission with a single maximum at about 487 nm. The bmFP was also active in lieu of FMN in the NADH/FMN oxidoreductase (flavin reductase)-luciferase coupled bioluminescence reaction in the absence of added FMN. In the coupled reaction, bioluminescence with the isolated bmFP chromophore was weaker than that with the holo-bmFP. After bmFP was used in luciferase reactions initiated either chemically or electrochemically, it was still capable of emitting bimodal fluorescence.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan. karatani@ipc.kit.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Karatani
authors.forename: T
authors.initials: T
authors.lastname: Konaka
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D019281:Dimerization; D005486:Flavin Mononucleotide / Q000378:metabolism; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism*; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism*; D046911:Macromolecular Substances; D010776:Photobacterium / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D046911:Macromolecular Substances; C404984:bimodal fluorescent protein, Photobacterium phosphoreum; D005486:Flavin Mononucleotide; D008156:Luciferases