Functional reconstitution of the Na(+)-driven polar flagellar motor component of Vibrio alginolyticus.
The bacterial flagellar motor is a molecular machine that couples the influx of specific ions to the generation of the force necessary to drive rotation of the flagellar filament. Four integral membrane proteins, PomA, PomB, MotX, and MotY, have been suggested to be directly involved in torque generation of the Na(+)-driven polar flagellar motor of Vibrio alginolyticus. In the present study, we report the isolation of the functional component of the torque-generating unit. The purified protein complex appears to consist of PomA and PomB and contains neither MotX nor MotY. The PomA/B protein, reconstituted into proteoliposomes, catalyzed (22)Na(+) influx in response to a potassium diffusion potential. Sodium uptake was abolished by the presence of Li(+) ions and phenamil, a sodium channel blocker. This is the first demonstration of a purification and functional reconstitution of the bacterial flagellar motor component involved in torque generation. In addition, this study demonstrates that the Na(+)-driven motor component, PomA and PomB, forms the Na(+)-conducting channel.
Sato
K
K
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
Homma
M
M
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D002850:Chromatography, Gel; D005407:Flagella / Q000737:chemistry*; D007700:Kinetics; D011188:Potassium / Q000378:metabolism; D017433:Protein Structure, Secondary; D011510:Proteolipids / Q000737:chemistry; D012964:Sodium / Q000378:metabolism* / Q000493:pharmacokinetics; D013997:Time Factors; D019415:Torque; D014733:Vibrio / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins; C103352:PomA protein, bacteria; C103353:PomB protein, bacteria; D011510:Proteolipids; C020485:proteoliposomes; D012964:Sodium; D011188:Potassium