[The application of D1S80 locus analysis to forensic problems by using Amp-FLP technique].
This is a paper on paternity testing in 85 test cases and the identification of individuals from mixed stains of semen and vaginal secretion in two rape cases. We used primers flanking the hypervariable region of the D1S80 gene to amplify DNA extracted from fresh blood, blood stain, saliva stain, semen stain, mixed stain in vaginal swab, and from fetal tissues. The results revealed that 35 out of 85 alleged fathers in 85 paternity testing cases were excluded. In 30 of the 35 excluded cases, the alleged fathers were excluded by D1S80 locus analysis. The exclusion rate was 35.29%. In 2 of the excluded cases, the alleged fathers were excluded by D1S80 locus analysis only. In 50 nonexcluded paternity testing cases, the paternity index(PI) of D1S80 locus was 2. 0576-111.1111. It indicates that D1S80 locus analysis plays an important role in elevation the relative chance of paternity (RCP) value. In 41 of the 50 nonexcluded cases, RCP was higher that 99.75%. RCP reached 99.300%-99.649% in 9 of cases. In one rape and homicide case, the genotype of D1S80 locus of sperm on the vaginal swab was detected. It gave a clue to investigation. In the other rape case, the genotype of D1S80 of sperm on the vaginal swab was consistent with the blood stain from the suspect. So the suspect was not excluded. Since the D1S80 locus has a high discrimination power it is very useful for forensic individual identification and parentage testing. Some problems have been discussed in this paper.
Sun
G
G
Department of Forensic Biology, Chengdu.
Jing
Q
Q
Chen
L
L
Wu
M
M
D001801:Blood Stains; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D005554:Forensic Medicine; D005838:Genotype; D006197:Hair / Q000737:chemistry; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011902:Rape; D012463:Saliva / Q000737:chemistry; D012661:Semen / Q000737:chemistry
D004247:DNA