title: Suppression of GD1alpha ganglioside-mediated tumor metastasis by liposomalized WHW-peptide.
abstract: GD1alpha ganglioside-replica peptides were recently isolated from a phage-displayed random pentadecapeptide library by assaying for inhibition of adhesion of RAW117-H10 lymphosarcoma cells to hepatic sinusoidal microvessel endothelial (HSE) cells. We show here that the Trp-His-Trp (WHW) peptide was identified as a minimal sequence of the GD1alpha-replica peptide WHWRHRIPLQLAAGR. The addition of WHW peptide-attached liposomes displayed efficient inhibition of liver metastasis of RAW117-H10 cells as well as of GD1alpha-mediated adhesion of RAW117-H10 cells to HSE cells in vitro. These results suggest that engineered liposomes for peptide delivery are applicable to treatment for metastasis.
authors.affiliation: Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takikawa
authors.forename: H
authors.initials: H
authors.lastname: Kikkawa
authors.forename: T
authors.initials: T
authors.lastname: Asai
authors.forename: N
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: D
authors.initials: D
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Ogino
authors.forename: T
authors.initials: T
authors.lastname: Taki
authors.forename: N
authors.initials: N
authors.lastname: Oku
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D004337:Drug Carriers; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors; D008081:Liposomes; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D010455:Peptides / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D004337:Drug Carriers; C404945:GD1alpha-replica peptide; D008081:Liposomes; D010455:Peptides; D011506:Proteins; C064020:ganglioside GD1alpha; D005677:G(M1) Ganglioside