[The effects of hydroxyurea on cell-cycle distribution and the expression of human beta-globin gene in K 562 cells].
Although the hydroxyurea (HU) has been extensively studied, little is known of its molecular mechanism in controlling the expression of human globin gene and in modulating the progression of cell-cycle in K 562 cell. In the present study, the effect of hydroxyurea on proliferative kinetics of K 562 cells was examined by monitoring the number of cells during a period of 8 day's cell culture. Our results showed that there was a dose related decrease in cell growth when K562 cells were incubated with HU. Moreover, cell-cycle analysis demonstrated that HU had profound effect on cell-cycle distribution. In the case of the induced K 562 cells, there was an increased accumulation of cells in S phase and a decreased fraction of cells in G 1 and G 2 + M phase. Furthermore, HU could induce the expression of human beta-globin gene in the induced K 562 cells. Our results indicate that HU has a potential to inhibit the proliferation of K 562 cells and to stimulate the terminal differentiation of this cell.
Jiang
C
C
Shanghai Institute of Cell Biology, Academy Sinica, China.
Dai
C H
CH
Xie
H Y
HY
Chen
Y D
YD
Wu
W
W
Jun
Z P
ZP
Qian
R L
RL
D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D020014:K562 Cells / Q000378:metabolism / Q000473:pathology
D005914:Globins; D006918:Hydroxyurea