Antioxidative activity of natural products from plants.
A variety of flavonoids, lignans, an alkaloid, a bisbenzyl, coumarins and terpenes isolated from Chinese herbs was tested for antioxidant activity as reflected in the ability to inhibit lipid peroxidation in rat brain and kidney homogenates and rat erythrocyte hemolysis. The pro-oxidant activities of the aforementioned compounds were assessed by their effects on bleomycin-induced DNA damage. The flavonoids baicalin and luteolin-7-glucuronide-6'-methyl ester, the lignan 4'-demethyldeoxypodophyllotoxin, the alkaloid tetrahydropalmatine, the bisbenzyl erianin and the coumarin xanthotoxol exhibited potent antioxidative activity in both lipid peroxidation and hemolysis assays. The flavonoid rutin and the terpene tanshinone I manifested potent antioxidative activity in the lipid peroxidation assay but no inhibitory activity in the hemolysis assay. The lignan deoxypodophyllotoxin, the flavonoid naringin and the coumarins columbianetin, bergapten and angelicin slightly inhibited lipid peroxidation in brain and kidney homogenates. It is worth stressing that the compounds with antioxidant effects in this assay, with the exception of tetrahydropalmatin and tanshinone I, have at least one free aromatic hydroxyl group in structure. Obviously, the aromatic hydroxyl group is very important for antioxidative effects of the compounds. None of the compounds tested exerted an obvious pro-oxidant effect.
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Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT. liufangzk@hotmail.com
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D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D001923:Brain Chemistry / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects; D005609:Free Radicals / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D007202:Indicators and Reagents; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010944:Plants / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D000975:Antioxidants; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005609:Free Radicals; D007202:Indicators and Reagents; D013729:Terpenes; D001761:Bleomycin; D008315:Malondialdehyde