Carboxymethylated glucan inhibits lipid peroxidation in liposomes.
Protective capabilities were studied of carboxymethylated (1-->3)-beta-D-glucan from Saccharomyces cerevisiae cell wall against lipid peroxidation in phosphatidylcholine liposomes induced by OH radicals produced with Fenton's reagent (H2O2/Fe2+) and also by microwave radiation using absorption UV-VIS spectrophotometry. A significant decrease in the conjugated diene production, quantified as Klein oxidation index, was observed in the presence of a moderate amount of added glucan. Increase of the oxidation index was accompanied with enhanced carboxyfluorescein leakage as a result of liposome membrane destabilization. This process was markedly suppressed with glucan present in the liposome suspension. Therefore, glucan may be considered as a potent protector against microwave radiation-induced cell damage.
Babincová
M
M
Department of Biophysics and Chemical Physics, Comenius University, Bratislava, Slovak Republic.
Machová
E
E
Kogan
G
G
D002473:Cell Wall / Q000737:chemistry; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D017665:Hydroxyl Radical / Q000032:analysis; D007700:Kinetics; D015227:Lipid Peroxidation; D008081:Liposomes; D008872:Microwaves; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D047071:beta-Glucans
D005936:Glucans; D008081:Liposomes; D047071:beta-Glucans; D017665:Hydroxyl Radical; C029933:carboxymethyl-beta-1,3-glucan