Progestins and androgens increase expression of Spot 14 in T47-D breast tumor cells.
Enhanced expression of fatty acid synthase and other lipogenic enzymes has been observed in a subset of breast cancers with poor prognosis. This phenomenon has been related to amplification of a gene on chromosome region 11q13 encoding Spot 14, a putative regulator of lipogenic enzyme expression. In this paper we demonstrate that the induction of lipogenesis by progestins and androgens in the breast cancer cell line T47-D is accompanied by a marked increase in the expression of Spot 14. These data corroborate the correlation between Spot 14 expression and increased lipogenesis. Moreover they show that apart from gene amplification there is another steroid-regulated pathway that may enhance Spot 14 expression and lipogenesis in tumor cells.
Heemers
H
H
Laboratory for Experimental Medicine and Endocrinology, Faculty of Medicine, Onderwijs en Navorsing, Gasthuisberg, Catholic University of Leuven, Herestraat 49, Leuven, B-3000, Belgium.
Vanderhoydonc
F
F
Heyns
W
W
Verhoeven
G
G
Swinnen
J V
JV
D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism*; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D013196:Dihydrotestosterone / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000096:biosynthesis; D015741:Metribolone / Q000494:pharmacology; D009376:Neoplasms, Hormone-Dependent / Q000235:genetics* / Q000378:metabolism*; D009687:Nuclear Proteins; D011282:Pregnenediones / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D045167:Progesterone Congeners / Q000494:pharmacology*; D011506:Proteins / Q000235:genetics*; D045165:Testosterone Congeners / Q000494:pharmacology*; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D008055:Lipids; D009687:Nuclear Proteins; D011282:Pregnenediones; D045167:Progesterone Congeners; D011506:Proteins; C051065:THRSP protein, human; D045165:Testosterone Congeners; D014157:Transcription Factors; D013196:Dihydrotestosterone; C020175:16 alpha-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione; D015741:Metribolone; D011374:Progesterone