Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis.
Mycobacterium tuberculosis thymidylate kinase complexed with the substrate deoxythymidine monophosphate was crystallized in the hexagonal space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 76.62, c = 134.38 A and one single monomer of 23 kDa in the asymmetric unit. Cryo-cooled crystals diffract at 1.94 A resolution using synchrotron radiation.
Li de la Sierra
I
I
Unité de Biochimie Structurale, Institut Pasteur, 28 Rue du Dr Roux, 75724, Paris CEDEX 15, France.
Munier-Lehmann
H
H
Gilles
A M
AM
Bârzu
O
O
Delarue
M
M
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D003460:Crystallization; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D009703:Nucleoside-Phosphate Kinase / Q000737:chemistry* / Q000378:metabolism; D013938:Thymidine Monophosphate / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D013938:Thymidine Monophosphate; D009703:Nucleoside-Phosphate Kinase; C020093:dTMP kinase