title: Liquid chromatographic determination of vanillin and related aromatic compounds.
abstract: This paper describes a reversed-phase liquid chromatographic method for the determination of vanillin, associated natural aromatic compounds and/or synthetic precursors, ethyl vanillin, and coumarin, a commonly encountered adulterant in nonbeverage and beverage alcohol products using a ternary gradient mobile phase. The compounds were separated on a Nova-Pak C18 column by using water, methanol, and tetrahydrofuran as the mobile phase. Measurements were made by using a photodiode array detector at 275 nm. The choice of the mobile phase and the column provides baseline resolution of vanillin and the associated aromatic compounds commonly found in vanilla-flavoring material. Because this method provides low-level detection/quantitation, it is suitable for the characterization of vanilla flavoring materials that are currently added to vanilla flavored beverage alcohol products.
authors.affiliation: U.S. Department of the Treasury, National Laboratory Center, Rockville, MD 20850-3188, USA.
authors.forename: E
authors.initials: E
authors.lastname: Jagerdeo
authors.forename: E
authors.initials: E
authors.lastname: Passetti
authors.forename: S M
authors.initials: SM
authors.lastname: Dugar
mesh_terms: D000434:Alcoholic Beverages / Q000032:analysis*; D001547:Benzaldehydes / Q000032:analysis*; D002110:Caffeine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis; D005421:Flavoring Agents / Q000032:analysis*
chemical_list: D001547:Benzaldehydes; D003374:Coumarins; D005421:Flavoring Agents; D002110:Caffeine; C030123:coumarin; C100058:vanillin; C045941:ethyl vanillin