Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774.
Nitrite reductase from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 is a multihaem (type c) membrane-bound enzyme that catalyzes the dissimilatory conversion of nitrite to ammonia. Crystals of the oxidized form of this enzyme were obtained using PEG and CaCl(2) as precipitants in the presence of 3--(decylmethylammonium)propane-1-sulfonate and belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 78.94, b = 104.59, c = 143.18 A. A complete data set to 2.30 A resolution was collected using synchrotron radiation at the ESRF. However, the crystals may diffract to beyond 1.7 A and high-resolution data will be collected in the near future.
Dias
J M
JM
Departamento de Química, Centro de Química Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2825-114 Monte de Caparica, Portugal.
Cunha
C A
CA
Teixeira
S
S
Almeida
G
G
Costa
C
C
Lampreia
J
J
Moura
J J
JJ
Moura
I
I
Romão
M J
MJ
D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003460:Crystallization; D003901:Desulfovibrio / Q000145:classification / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D009572:Nitrite Reductases / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012967:Sodium Dodecyl Sulfate; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate; D009572:Nitrite Reductases