Membrane association of the Escherichia coli enterobactin synthase proteins EntB/G, EntE, and EntF.
The cytosolic proteins EntE, EntF, and EntB/G, which are Escherichia coli enzymes necessary for the final stage of enterobactin synthesis, are released by osmotic shock. Here, consistent with the idea that cytoplasmic proteins found in shockates have an affinity for membranes, a small fraction of each was found in membrane preparations. Two procedures demonstrated that the enzymes were enriched in a minor membrane fraction of buoyant density intermediate between that of cytoplasmic and outer membranes, providing indirect support for the notion that these proteins have a role in enterobactin excretion as well as synthesis.
Hantash
F M
FM
Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095, USA.
Earhart
C F
CF
D002462:Cell Membrane / Q000201:enzymology*; D004758:Enterobactin / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins; D006867:Hydrolases / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D010453:Peptide Synthases / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology
D029968:Escherichia coli Proteins; D004758:Enterobactin; D006867:Hydrolases; C064012:isochorismatase; D008025:Ligases; D010453:Peptide Synthases; C026269:2,3-dihydroxybenzoate - serine ligase; C061746:2,3-dihydroxybenzoate-AMP ligase, E coli