Cre-mediated cerebellum- and hippocampus-restricted gene mutation in mouse brain.
Using the phage P1-derived Cre/loxP recombination system, we have created a line of cre-transgenic mice in which the Cre-mediated gene deletion is restricted to granule cells of cerebellum and dentate gyrus of hippocampus. Low levels of deletion were also present in pyramidal cells of hippocampal CA1 and CA3 fields. The Cre/loxP recombination occurred prenatally. The recombination efficiencies in the granular layer of the cerebellum, the granular layer of the dentate gyrus, and the CA1 and CA3 pyramidal cells of the hippocampus were 34.0%, 23.1%, 3.0%, and 9.8%, respectively. This line of cre-transgenic mice should be conducive to studies of the effect of a gene mutation upon brain development and plasticity.
Guo
H
H
Department of Molecular and Integrative Physiology, Neuroscience Program and Beckman Institute of Advanced Science and Technology, Urbana, Illinois 61801, USA.
Mao
C
C
Jin
X L
XL
Wang
H
H
Tu
Y T
YT
Avasthi
P P
PP
Li
Y
Y
D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D018390:Gene Targeting; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009154:Mutation; D011995:Recombination, Genetic; D014157:Transcription Factors; D014764:Viral Proteins; D000099260:Empty Spiracles Homeobox Proteins
D017931:DNA Primers; D018398:Homeodomain Proteins; D019426:Integrases; D014157:Transcription Factors; D014764:Viral Proteins; D000099260:Empty Spiracles Homeobox Proteins; C045073:Cre recombinase