Age-dependent increase in C7-1 gene expression in rat frontal cortex.
We have previously reported that a fragment of mRNA, denoted as C7-1, which expression was significantly increased in the frontal cortex of aged rats. In the present study, we have cloned and sequenced the full length cDNA of the C7-1 gene. We have found that the open reading frame of this gene encoded a 463-amino-acid protein, which shared 84% identity in amino acid sequence with a subunit of vacuolar H(+)-ATPase (V-ATPase). Further Northern blot analysis revealed that there was an age-dependent increase in C7-1 gene expression in rat frontal cortex, but not in other brain areas. Moreover, application of C7-1 antisense oligonucleotide to cortical neuronal cultures markedly inhibited cell survival. These results together suggest that C7-1 is a marker for the aging process and that upregulation of C7-1 may be important in maintaining the normal function of V-ATPase during aging.
Hung
H
H
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Tsai
M J
MJ
Wu
H C
HC
Lee
E H
EH
D000375:Aging / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015415:Biomarkers; D015152:Blotting, Northern; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D025262:Vacuolar Proton-Translocating ATPases
C406669:Atp6ap1 protein, rat; D015415:Biomarkers; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D011506:Proteins; D012333:RNA, Messenger; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases