Capnocytophaga ochracea: characterization of a plasmid-encoded extended-spectrum TEM-17 beta-lactamase in the phylum Flavobacter-bacteroides.
A plasmid-encoded extended-spectrum TEM beta-lactamase with a pI of 5.5 was detected in a Capnocytophaga ochracea clinical isolate. The bla gene was associated with a strong TEM-2 promoter and was derived from bla(TEM-1a) with a single-amino-acid substitution: Glu(104)-->Lys, previously assigned to TEM-17, which is thus the first TEM beta-lactamase to be reported in the phylum Flavobacter-Bacteroides.
Rosenau
A
A
Département de Microbiologie Médicale et Moléculaire, Centre Hospitalier Universitaire Bretonneau, 37044 Tours cedex, France. rosenau@med.univ-tours.fr
Cattier
B
B
Gousset
N
N
Harriau
P
P
Philippon
A
A
Quentin
R
R
D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002206:Capnocytophaga / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D007526:Isoelectric Point; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases