title: Biocompatibility of an adhesive system applied to exposed human dental pulp.
abstract: Human pulp tissue was directly capped with All Bond 2, or calcium hydroxide and evaluated 7, 30, or 60 days after the procedures. Histological analysis was performed to assess the inflammatory cell response, tissue disorganization, dentin bridging, and the presence of bacteria. At 7 days, with All Bond 2 capping, there was a large area of neutrophilic infiltrate underlying the pulp capping material, and the death of adjacent odontoblasts, was observed. However, with time, the neutrophilic reaction was replaced by fibroblastic proliferation with macrophages and giant cells surrounding globules of resin scattered in the coronal pulp tissue. The persistent inflammatory reaction and hyaline alteration of extracellular matrix inhibited complete pulp repair or dentin bridging. In contrast, at 7 days, the pulp tissue capped with calcium hydroxide exhibited odontoblast-like cells organized underneath coagulation necrosis. Pulp repair evolved into apparent complete dentin bridge formation at 60 days. All Bond 2 did not appear to allow any pulp repair and does not appear to be indicated for direct pulp capping of human teeth.
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, Araraquara Dental School, University of São Paulo State, Araraquara, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Hebling
authors.forename: E M
authors.initials: EM
authors.lastname: Giro
authors.forename: C A
authors.initials: CA
authors.lastname: Costa
mesh_terms: D000293:Adolescent; D001672:Biocompatible Materials / Q000633:toxicity; D002126:Calcium Hydroxide / Q000494:pharmacology; D002648:Child; D003782:Dental Pulp / Q000187:drug effects*; D003785:Dental Pulp Capping / Q000379:methods*; D003790:Dental Pulp Necrosis / Q000139:chemically induced; D003809:Dentin, Secondary / Q000187:drug effects; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D005109:Extracellular Matrix / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000633:toxicity*; D011671:Pulpitis / Q000139:chemically induced
chemical_list: D001672:Biocompatible Materials; D017220:Dentin-Bonding Agents; D008689:Methacrylates; C100742:All-Bond 2; D002126:Calcium Hydroxide