Identification of the zinc-binding protein specifically present in male rat liver as carbonic anhydrase III.
A zinc (Zn)-binding protein that is present specifically in the livers of male adult rats was detected by HPLC with in-line detection by mass spectrometry (ICP MS). The Zn-binding protein was purified on Sephadex G-75 and G3000SW HPLC columns. and was identified as carbonic anhydrase III (CAIII) based on the amino acid sequence of a peptide obtained on lysyl endopeptidase digestion. CAIII is expressed as one of the major Zn-binding proteins in the livers of male rats in an age-dependent manner, a comparable amount of Zn to that of copper, Zn-superoxide dismutase (Cu,Zn-SOD) being bound to CAIII at 8 weeks of age. Castration at 4 or 8 weeks of age was shown to reduce Zn bound to CAIII to 47.5% of the sham-operated control level, suggesting that the sex-dependent expression of CAIII is partly regulated by a sex hormone, androgen. The concentration of CAIII in the livers of Long-Evans rats with a cinnamon-like coat color (LEC rats), an animal model of Wilson disease, was also estimated as Zn bound to CAIII and shown to be lower than that in Wistar rats before the onset of hepatitis. The concentration of CAIII was decreased specifically by repeated injections of cupric ions without the Cu,Zn-SOD concentration being affected.
Suzuki
K T
KT
Faculty of Pharmaceutical Sciences, Chiba University, Inage, Japan. ktsuzuki@p.chiba-u.ac.jp
Takenaka
J
J
Ogra
Y
Y
D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000172:deficiency / Q000494:pharmacology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008982:Molybdenum / Q000494:pharmacology; D009919:Orchiectomy; D051381:Rats; D020332:Rats, Inbred LEC; D017208:Rats, Wistar; D012727:Sex Characteristics; D013045:Species Specificity; D015032:Zinc / Q000032:analysis
D002352:Carrier Proteins; C017800:zinc-binding protein; D003300:Copper; D008982:Molybdenum; C020809:tetrathiomolybdate; D002256:Carbonic Anhydrases; D015032:Zinc