Immobilization of tumoral pancreatic islet cells on a two-dimensional microsupport.
A new procedure for the immobilization of tumoral pancreatic islet cells to a two-dimensional microsupport is presented. Tumoral islet cells of the RINm5F line (0.7x10(6) cells/ml) were immobilized to two-dimensional microcarriers (16.6 cm2/ml). Within 24 h of culture, and as judged from the number of cells, their protein or insulin content, less than 10% of the cells escaped immobilization. The metabolic response of the immobilized cells to D-glucose was well preserved, the paired ratio between D-[U-14C] glucose oxidation and D-[5-3H]glucose utilization being even significantly higher in immobilized than free cells. The advantages of this novel approach in the perspective of islet cell transplantation are underlined.
Olivares
E
E
Laboratory of Experimental Medicine, Brussels Free University, Brussels, Belgium.
Malaisse
W J
WJ
D018929:Cell Culture Techniques; D018914:Cells, Immobilized; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D007328:Insulin; D005947:Glucose