Postnatal profiles of myogenic regulatory factors and the receptors of TGF-beta 2, LIF and IGF-I in the gastrocnemius and rectus femoris muscles of dy mouse.
The dystrophin-deficient mdx mouse presents muscle fiber necrosis but active muscle regeneration, probably due to an extensive recruitment of myogenic regulatory factors (MRF), several growth factors and cytokines, and favorable interaction of satellite cells. In contrast, the laminin alpha 2 (merosin)-deficient dy mouse shows progressive muscle fiber necrosis and ineffective muscle regeneration. Using Western blot and immunohistochemical analyses, we investigated the adaptive changes in MRF, growth factors and cytokines and their receptors in the muscles of dy mice during postnatal growth. The relative volume of MyoD, myogenin and Myf-5 proteins was markedly lower in the gastrocnemius and rectus femoris muscles of dy mice. Transforming growth factor-beta 2, leukemia inhibitory factor (LIF) and basic fibroblast growth factor were not up-regulated in the muscles of dy mice. The levels of the LIF receptor and insulin-like growth factor-I receptor levels were markedly decreased in the muscles of dy mice during the entire postnatal period observed in this study. Therefore, unlike the situation in mdx mice, the milieu of regeneration following repetitive damage seems to be degraded in the muscles of dy mice.
Sakuma
K
K
Department of Physiology, Aichi Human Service Center, Japan. sakuma@inst-hsc.pref.aichi.jp
Watanabe
K
K
Sano
M
M
Uramoto
I
I
Totsuka
T
T
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D004268:DNA-Binding Proteins; D016189:Dystrophin / Q000235:genetics*; D006131:Growth Inhibitors / Q000032:analysis*; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000473:pathology / Q000502:physiology; D009124:Muscle Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000473:pathology*; D017570:MyoD Protein / Q000032:analysis; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin / Q000032:analysis; D009336:Necrosis; D017526:Receptor, IGF Type 1 / Q000032:analysis; D018121:Receptors, Cytokine / Q000032:analysis; D053606:Receptors, OSM-LIF; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis*; D012038:Regeneration; D015534:Trans-Activators; D016212:Transforming Growth Factor beta / Q000032:analysis*
D004268:DNA-Binding Proteins; D016189:Dystrophin; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507838:Lif protein, mouse; C507841:Lifr protein, mouse; D008222:Lymphokines; D009124:Muscle Proteins; C497418:Myf5 protein, mouse; D017570:MyoD Protein; C503801:Myog protein, mouse; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D018125:Receptors, Transforming Growth Factor beta; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1