title: Use of two-dimensional electrophoresis to evaluate proteolysis in salmon (Salmo salar) muscle as affected by a lactic fermentation.
abstract: Two-dimensional electrophoresis was used to study proteolysis in salmon fillets inoculated or not with the starter culture Lactobacillus sake LAD. Protein fragments appeared increasingly with time in both samples, indicating that the main quantitative changes were due to endogenous enzymes. In the most acidic zone (pI = 4-6. 20) particularly, proteolysis was overall independent from processing. In contrast, fermentation had a significant effect in the pH range 6.20-8.35, suggesting a specificity of the bacterial proteases of L. sake toward alkaline to slightly acidic proteins. Furthermore, fragments surrounding tropomyosin (apparent pI = 4.70) appeared in fermented samples, indicating that the protein may be a suitable substrate for the metabolism of L. sake LAD.
authors.affiliation: Department of Food Technology and National Food Biotechnology Centre, University College Cork, Cork City, Ireland.
authors.forename: M
authors.initials: M
authors.lastname: Morzel
authors.forename: V
authors.initials: V
authors.lastname: Verrez-Bagnis
authors.forename: E K
authors.initials: EK
authors.lastname: Arendt
authors.forename: J
authors.initials: J
authors.lastname: Fleurence
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005285:Fermentation; D007526:Isoelectric Point; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus; D009132:Muscles / Q000378:metabolism*; D020317:Salmo salar / Q000378:metabolism* / Q000382:microbiology
chemical_list: D019344:Lactic Acid