title: Activation of calpain precedes morphological alterations during hydrogen peroxide-induced apoptosis in neuronally differentiated mouse embryonal carcinoma P19 cell line.
abstract: In order to reveal neurodegeneration elicited by reactive oxygen intermediates (ROI), neuronally differentiated cells from mouse embryonal carcinoma P19 cell line were exposed to hydrogen peroxide (H2O2). Enhanced protein-phosphorylation on tyrosine residues was detectable within 5 min of exposure to H2O2, and gradual rises in intracellular free Ca2+ level and in calpain activity were observed. Furthermore, H2O2 stimulation of differentiated P19 cells for 24 h resulted in morphological alterations in somas as well as neurites. Also, within 6 h of H2O2 treatment DNA fragmentation has been detected. Taken together, these results suggest that oxidative stress induces degradation of cytoskeletal proteins presumably resulting from increased intracellular Ca2+ concentration and subsequent activation of calpain.
authors.affiliation: Department of Biochemistry, Kobe University School of Medicine, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Ishihara
authors.forename: Y
authors.initials: Y
authors.lastname: Minami
authors.forename: T
authors.initials: T
authors.lastname: Nishizaki
authors.forename: T
authors.initials: T
authors.lastname: Matsuoka
authors.forename: H
authors.initials: H
authors.lastname: Yamamura
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002154:Calpain / Q000378:metabolism*; D018236:Carcinoma, Embryonal; D002454:Cell Differentiation; D004789:Enzyme Activation; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress; D010750:Phosphoproteins / Q000378:metabolism; D019000:Phosphotyrosine; D014407:Tumor Cells, Cultured
chemical_list: D010750:Phosphoproteins; D019000:Phosphotyrosine; D006861:Hydrogen Peroxide; D002154:Calpain; D002118:Calcium