title: Protective effects of Ginkgo biloba extract on cultured rat cardiomyocytes damaged by H2O2.
abstract: OBJECTIVE
  To investigate the influence of Ginkgo biloba extract (GbE) on cardiomyocytes damaged by H2O2.
  
  
  METHODS
  Cultured rat cardiomyocytes were divided into 3 groups randomly: control group; H2O2 (2.5 mmol.L-1) group; H2O2 2.5 mmol.L-1 + GbE 150 mg.L-1 group. The cardiomyocytes were cultured in MEM (Eagle's) at 37 degrees C in the presence of 5% CO2 for 4 h. Lactate dehydrogenase (LDH) was assayed by colorimetric method. Lipid peroxidation was determined by measuring thiobarbituric acid-reactive substances. Ultrastructure was viewed under transmission electron microscope.
  
  
  RESULTS
  Compared with the control group, LDH leakage and malondialdehyde (MDA) content increased in H2O2 group, LDH increased from (2166 +/- 247) U.L-1 to (5180 +/- 648) U.L-1, MDA increased from (3.5 +/- 0.2) nmol/10(6) cells to (7.2 +/- 0.4) nmol/10(6) cells (P < 0.01). The ultrastructure was damaged seriously. GbE inhibited the increase of LDH leakage and MDA content induced by H2O2. In this group, LDH decreased from (5180 +/- 648) U.L-1 to (3496 +/- 386) U.L-1, MDA decreased from (7.2 +/- 0.4) nmol/10(6) cells to (4.8 +/- 0.9) nmol/10(6) cells (P < 0.01). Ultrastructure of cells was also protected by GbE.
  
  
  CONCLUSIONS
  GbE protected the cardiomyocyte against H2O2 injury, the protective action was attributed to its antiperoxidative effect.
authors.affiliation: Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospitol, Shanghai Medical University, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Niu
authors.forename: X Y
authors.initials: XY
authors.lastname: Yang
authors.forename: W S
authors.initials: WS
authors.lastname: Bao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D010946:Plants, Medicinal; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase