[Study on double antigens sandwich enzyme immunoassay for detection of Brucella specific antibodies in human and animals].
OBJECTIVE
In order to develop practicable methods for serodiagnosis, surveillance and epidemiological surveys in human and animals Brucellosis.


METHODS
A double antigens sandwich Enzyme Immunoassay (DAgS-EIA include DAgS-ELISA and DAgS-DIEA) have been developed under the basis of first production conjugate of Brucella antigen with horseradish peroxidase. Serum samples from diagnosed patients, suspected patients of Brucellosis and sheep infected with Brucella in Henan and Hebei provinces as well as from experimental animals of infected with Brucella in laboratory detected through DAgS-EIA, I-ELISA, RBPT and SAT tests.


RESULTS
Of the diagnosed and suspected Brucellosis patients, positive rates of DAgS-ELISA, RBPT, I-ELISA, DAgS-DIEA and SAT were 60.0%-62.9%, 48.6%-58.1%, 55.6%, 53.7% and 44.2% respectively; as for sheep with Brucella-infection, positive rate of the above mentioned 5 tests were 81.8%.


CONCLUSIONS
Results showed that DAgS-EIA were not only specific and practicable but may use for Brucella specific antibodies detection in humans and various animals with only one conjugate of Brucella antigen with horseradish peroxidase.
Lu
Q
Q
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Zhang
W
W
Hao
Z
Z
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis / Q000276:immunology* / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012698:Serologic Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology*
D000907:Antibodies, Bacterial