Effects of anordrin, droloxifene, nomegestrol, and mifepristone on cultured rat luteal cell apoptosis.
OBJECTIVE
To study the effect of four kinds of antifertility agents anordrin(Ano), droloxifene(Dro), nomegestrol (Nom), and mifepristone (Mif) on luteal cell apoptosis.


METHODS
Cultured rat luteal cells were incubated with different agents. HE stain was used to observe morphological changes. Extracted DNA was electrophoresed on agarose gel. Apoptotic cells were quantitated by flow cytometry.


RESULTS
All 4 drugs reduced cell viability. Dro induced apoptosis while the other 3 drugs induced necrosis. Typical DNA ladders were observed after cells were incubated with Dro and there were 15.4%, 75.4%, or 90.5% apoptotic cells after treatment with Dro 1.25, 2.5, or 3.75 mg.L-1, respectively.


CONCLUSIONS
Dro induced apoptosis while Ano, Nom, and Mif induced necrosis in cultured rat luteal cells.
Leng
Y
Y
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
Yang
B
B
Cao
L
L
Gu
Z P
ZP
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003280:Contraceptives, Oral, Synthetic / Q000494:pharmacology; D003338:Corpus Luteum / Q000166:cytology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D008186:Luteolytic Agents / Q000494:pharmacology; D008535:Megestrol / Q000031:analogs & derivatives* / Q000494:pharmacology; D015735:Mifepristone / Q000494:pharmacology*; D009635:Norandrostanes / Q000494:pharmacology*; D045167:Progesterone Congeners / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000494:pharmacology
D003280:Contraceptives, Oral, Synthetic; D004965:Estrogen Antagonists; D008186:Luteolytic Agents; D009635:Norandrostanes; D045167:Progesterone Congeners; D013629:Tamoxifen; C038345:droloxifene; C047637:nomegestrol; D015735:Mifepristone; C010855:anordrin; D008535:Megestrol