[Experimental study of effects of anti-ovarian antibodies on ovarian histology and function].
OBJECTIVE
To investigate the effects of anti-ovarian antibodies (AOA) on ovarian histology and functions.


METHODS
Rabbit antibodies against mice ovarian tissues were obtained by immunizing with mice ovarian extracts and purified. The effects of AOA on mice ovarian histology were examined under light and electronic microscope. In addition, changes of ovarian functions, including natural pregnant rate and pregnant mare serum gonadotropin (PMSG) + hCG induced ovulation rate and pregnant rate, were also observed.


RESULTS
After treating with different doses of AOA pathological changes occurred in a variety of ovarian components, especially in zona pellucida and granulosa cells, which is more serious in high AOA level group than that in low AOA level group. The natural pregnant rate decreased to zero in both AOA groups. The ovulation and pregnant rate induced by PMSG-hCG were significant lower in both AOA groups than that in the control group especially in the high AOA level group.


CONCLUSIONS
AOA may damage the ovarian tissues and reduce the ovulation and pregnant rate. The higher the AOA level, the more serious pathological changes and the poorer curative effects of PMSG-hCG may occur.
Lin
J
J
Renji Hospital, Shanghai Second Medical University.
Yan
J
J
Lin
Q
Q
D000818:Animals; D001323:Autoantibodies / Q000494:pharmacology*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology; D005306:Fertilization / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010053:Ovary / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D010062:Ovulation Induction; D011817:Rabbits
D001323:Autoantibodies; D006063:Chorionic Gonadotropin; D005300:Fertility Agents, Female