title: Transduction of human GAD67 cDNA into immortalized striatal cell lines using an Epstein-Barr virus-based plasmid vector increases GABA content.
abstract: The M213-20 and M213-1L cell lines were immortalized from rat striatum using the tsA58 allele of the SV40 large T antigen, contain the GAD enzyme, and produce GABA (Giordano et al., 1994, Exp. Neurol. 124:395-400). Cell lines that produce large amounts of GABA may be useful for transplantation into the brain in conditions such as Huntington's disease or epilepsy, where localized application of GABA may be of therapeutic value. We have explored the potential use of the pREP10 plasmid vector, which replicates episomally, to increase GAD expression and GABA production in M213-20 and M213-1L cells. Human GAD(67) cDNA was transfected into M213-20 and M213-1L, and subclones were isolated with hygromycin selection. Immunochemical studies showed increased GAD(67) expression compared to the parent M213-20 and M213-1L cell lines. Staining for the EBNA antigen and Southern blots demonstrated that the pREP10 plasmid was stably maintained in the cells for at least 12-15 months in culture. Several clones were isolated in which GABA concentrations were increased by as much as 4-fold (M213-1L) or 44-fold (M213-20) compared to the parent cell lines or 12-fold (M213-1L) and 94-fold (M213-20) greater than rat striatal tissue (1.678 +/- 0.4 micromol/g prot). The ability of these cells to continue to produce large amounts of GABA while being maintained in culture for extended periods suggests that similar methods might be used with human cell lines to produce cells that can be transplanted into the brain to deliver GABA for therapeutic purposes.
authors.affiliation: Development & Plasticity Section, National Institute on Drug Abuse, Cellular Neurobiology Branch, 5500 Nathan Shock Drive, Baltimore, Maryland, 21224, USA.
authors.forename: C
authors.initials: C
authors.lastname: Conejero-Goldberg
authors.forename: C
authors.initials: C
authors.lastname: Tornatore
authors.forename: W
authors.initials: W
authors.lastname: Abi-Saab
authors.forename: M C
authors.initials: MC
authors.lastname: Monaco
authors.forename: O
authors.initials: O
authors.lastname: Dillon-Carter
authors.forename: M
authors.initials: M
authors.lastname: Vawter
authors.forename: J
authors.initials: J
authors.lastname: Elsworth
authors.forename: W
authors.initials: W
authors.lastname: Freed
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D018076:DNA, Complementary; D005822:Genetic Vectors; D005968:Glutamate Decarboxylase / Q000235:genetics* / Q000378:metabolism*; D004854:Herpesvirus 4, Human; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D014162:Transfection; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D011994:Recombinant Proteins; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1