Accelerated tissue regeneration through incorporation of basic fibroblast growth factor-impregnated gelatin microspheres into artificial dermis.
The objective of this study was to evaluate the effect of incorporation of basic fibroblast growth factor (bFGF)-impregnated gelatin microspheres into an artificial dermis on the regeneration of dermis-like tissues. When used in the free form in vivo, bFGF cannot induce sufficient wound healing activity, because of its short half-life. Therefore, sustained release of bFGF was achieved by impregnation into biodegradable gelatin microspheres. A radioisotope study revealed that incorporation of bFGF-impregnated gelatin microspheres significantly prolonged in vivo retention of bFGF in the artificial dermis. Artificial dermis with incorporated bFGF-impregnated gelatin microspheres or bFGF in solution was implanted into full-thickness skin defects on the back of guinea pigs (1.5 cm x 1.5 cm) (n = 4). Incorporation of bFGF into the artificial dermis accelerated fibroblast proliferation and capillary formation in a dose-dependent manner. However, the accelerated effects were more significant with the incorporation of bFGF-impregnated gelatin microspheres than with free bFGF at doses of 50 microg or higher. We conclude that the gelatin microsphere is a promising tool to accelerate bFGF-induced tissue regeneration in artificial dermis.
Kawai
K
K
Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Japan.
Suzuki
S
S
Tabata
Y
Y
Ikada
Y
Y
Nishimura
Y
Y
D000818:Animals; D001672:Biocompatible Materials; D002196:Capillaries / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005780:Gelatin; D006168:Guinea Pigs; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D012867:Skin / Q000098:blood supply / Q000166:cytology / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
D001672:Biocompatible Materials; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D005780:Gelatin