title: Determination of the lactate breakpoint during incremental exercise in horses adapted to dietary corn oil.
abstract: OBJECTIVE
  To determine lactate breakpoint of horses and test for effects of training and dietary supplementation with corn oil on that breakpoint.
  
  
  METHODS
  7 healthy Arabian horses.
  
  
  METHODS
  Horses received a control diet (n = 4) or a diet supplemented with 10% corn oil (4). A training program, which comprised two 5-week conditioning periods with 1 week of rest, was initiated. Submaximal incremental exercise tests (IET) were conducted before the first and after both conditioning periods. Blood samples for determination of blood lactate and plasma glucose concentrations were collected 1 minute before IET and during the 15 seconds immediately preceding each speed change. Data collected were fit to one- and two-slope broken-line models and an exponential model.
  
  
  RESULTS
  Good fits were obtained by application of the broken-line models (adjusted R2 > 0.92) to blood lactate concentration versus speed curves. Lactate breakpoints increased 41% after training. After training, slope 2 and peak blood lactate concentrations were greater in the corn oil group, compared with controls. Mean blood lactate concentration at the breakpoint was not affected by training or diet. Plasma glucose concentration versus speed curves also fit the broken-line models, and glucose breakpoints preceded lactate breakpoints by approximately 1 m/s in the second and third IET.
  
  
  CONCLUSIONS
  Lactate breakpoints can be determined for horses, using blood lactate concentration versus speed curves generated during submaximal IET and may be useful for assessing fitness and monitoring training programs in equine athletes.
authors.affiliation: Department of Animal and Poultry Science, Virginia Polytechnic Institute and State University, Blacksburg 24061-0306, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Kronfeld
authors.forename: S E
authors.initials: SE
authors.lastname: Custalow
authors.forename: P L
authors.initials: PL
authors.lastname: Ferrante
authors.forename: L E
authors.initials: LE
authors.lastname: Taylor
authors.forename: H D
authors.initials: HD
authors.lastname: Moll
authors.forename: T N
authors.initials: TN
authors.lastname: Meacham
authors.forename: W
authors.initials: W
authors.lastname: Tiegs
mesh_terms: D000821:Animal Feed; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003314:Corn Oil / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D006736:Horses / Q000502:physiology*; D007773:Lactates / Q000097:blood / Q000378:metabolism*; D008954:Models, Biological; D010805:Physical Conditioning, Animal / Q000502:physiology*; D012420:Running; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D004042:Dietary Fats, Unsaturated; D007773:Lactates; D003314:Corn Oil