Structure elucidation of glycoprotein glycans and of polysaccharides by NMR spectroscopy.
The applicability of 1H-NMR spectroscopy for the determination of the primary and tertiary structure of carbohydrate-containing molecules is demonstrated. For classes of known compounds the characterization can be based on chemical shifts observed in 1D NMR spectra with or without the aid of a computer database. For more complex structure determinations 2D NMR techniques are required. Here the application of 2D NMR is demonstrated for the primary structure determination of two bacterial exopolysaccharides, for the spatial structure determination of a disaccharide and a glycoprotein hormone.
Leeflang
B R
BR
Bijvoet Center for Biomolecular Research, Utrecht University, The Netherlands. b.r.leeflang@chem.uu.nl
Faber
E J
EJ
Erbel
P
P
Vliegenthart
J F
JF
D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011522:Protons; D012984:Software
D001426:Bacterial Proteins; D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D011135:Polysaccharides, Bacterial; D011522:Protons