title: Reconstruction of human hard-palate mucosal epithelium on de-epidermized dermis.
abstract: Artificial hard-palate mucosa equivalents were reconstructed using keratinocytes derived from normal human hard-palate and de-epidermized dermis. Reconstructed hard-palate mucosal epithelium formed in three-dimensional culture was compared to native hard-palate mucosal epithelium and reconstructed oral buccal mucosal epithelium with regard to keratin expression. Artificial hard-palate mucosal epithelium reconstructed in medium with delipidized serum showed a differentiation pattern similar to that of hard-palate epithelium in vivo. The present study also confirmed that keratinocytes derived from hard-palate mucosa are intrinsically different from those of nonkeratinizing oral surfaces.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, South Korea. khcho@snu.ac.kr
authors.forename: K H
authors.initials: KH
authors.lastname: Cho
authors.forename: H T
authors.initials: HT
authors.lastname: Ahn
authors.forename: K C
authors.initials: KC
authors.lastname: Park
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
authors.forename: S W
authors.initials: SW
authors.lastname: Kim
authors.forename: M W
authors.initials: MW
authors.lastname: Sung
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: P H
authors.initials: PH
authors.lastname: Chung
authors.forename: H C
authors.initials: HC
authors.lastname: Eun
authors.forename: J I
authors.initials: JI
authors.lastname: Youn
mesh_terms: D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D004817:Epidermis; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology*; D010159:Palate / Q000166:cytology*