title: Increased expression of VE-cadherin correlates temporally with differentiation of a restrictive endothelial barrier during normal angiogenesis in vivo.
abstract: The purpose of this study was to evaluate temporal expression of VE- and N-cadherins within the angiogenic chick chorioallantoic membrane (CAM). Whether their relative patterns of expression changed in conjunction with abrupt differentiation of the restrictive CAM endothelial barrier between days 4.5 and 5.0 of the 21 days gestation was evaluated. Immunoblots against VE-cadherin depicted an increase of VE-cadherin expression between days 4.5 and 5.0, but no change in expression was detected between days 5.0 and 6.0. N-cadherin expression, on the other hand, remained uniform from day 4.5 to day 6.0. Immunogold-labeled anti-VE-cadherin was found exclusively on the CAM endothelium, and principally along the lateral inter-endothelial junctions. Hence, VE-cadherin expression by the angiogenic endothelium was similar to that of adult endothelium. That VE-cadherin expression by the CAM endothelium was increased between days 4.5 and 5.0 serves to suggest a temporal correlation with the ontogeny of restrictive barrier function in angiogenic endothelium in vivo.
authors.affiliation: Department of Anatomy, Cell Biology and Injury Sciences, UMDNJ-New Jersey Medical School, Newark, USA.
authors.forename: A
authors.initials: A
authors.lastname: Cruz
authors.forename: D O
authors.initials: DO
authors.lastname: DeFouw
mesh_terms: D000482:Allantois / Q000098:blood supply / Q000166:cytology; D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000096:biosynthesis*; D002454:Cell Differentiation; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D018919:Neovascularization, Physiologic; D013997:Time Factors; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D000099152:Cadherin 5