A three-dimensional skin culture model for mouse keratinocytes: application to transgenic mouse keratinocytes.
The study of mouse epidermal biology has been hampered by the lack of a good in vitro model for the culture of mouse keratinocytes which allowed the reconstruction of a fully differentiated epidermis. We adapted the Pruniéras' model, also called the Dead de-Epidermized Dermis model (DED), to mouse keratinocytes and showed that a neo-epidermis can be reconstructed exhibiting a complete differentiation program. We also used this model to culture transgenic mouse keratinocytes. We observed that transgene expression occurred in the correct location and that the neo-epidermis mimed previous in vivo observations obtained with integrin skin-targeted transgenic mice. Therefore, this model will be a powerful tool to further investigate normal mouse and transgenic keratinocyte biology.
Carroll
J M
JM
Keratinocyte Laboratory, Imperial Cancer Research Fund, London, UK.
Molès
J P
JP
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000033:anatomy & histology / Q000254:growth & development; D039201:Integrin alpha3beta1; D016023:Integrins / Q000235:genetics; D015603:Keratinocytes / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D017469:Receptors, Fibronectin / Q000235:genetics; D012867:Skin / Q000033:anatomy & histology* / Q000166:cytology* / Q000254:growth & development
D039201:Integrin alpha3beta1; D016023:Integrins; D017469:Receptors, Fibronectin