[Conjugative transfer of a plasmid from Escherichia coli to various strains of the order Actinomycetales].
The conjugal transfer of autonomous and integrative plasmids from the donor strain Escherichia coli S17-1 to strains of genera Actinomadura, Arthrobacter, Kitasatoa, Micromonospora, Nocardia, Rhodococcus, Saccharopolyspora, and to 16 strains of the genus Streptomyces was demonstrated. The status of plasmids in recipient strains and the stability of their inheritance were analyzed. Plasmids constructed for strains of the genus Streptomyces were shown to function in a large number of strains belonging to the order Actinomycetales. The well-developed system of Streptomyces vector molecules and cloned genes of antibiotic biosynthesis allows their transfer to those microorganisms for which conventional techniques of plasmid transfer by regenerated protoplast transformation or electroporation have not been developed or are inefficient.
Voeĭkova
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TA
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia. lomov@vnigen.msk.su
D000192:Actinomycetales / Q000235:genetics*; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics*; D018014:Gene Transfer Techniques; D010957:Plasmids; D013045:Species Specificity