Phytochelatin homologs induced in hairy roots of horseradish.
When exposed to excess heavy metals, plants induce phytochelatins and related peptides (all designated as PCAs). Thus, when hairy roots of horseradish (Armoracia rusticana) were exposed for 3 days to cadmium (1 mM) along with reduced glutathione (2 mM), PCA induction occurred. Moreover, a new family of thiol peptides was detected as well as the previously known PCAs, as revealed by postcolumn-derivatization HPLC. Two were isolated and their structures were identified as (gamma-Glu-Cys)n-Gln (n = 3 and 4) by electrospray ionization-mass spectrometer spectra, this being confirmed by chemical synthesis of the peptides. These new analogs constitute the sixth PCA family identified to date.
Kubota
H
H
National Institute of Health Sciences, Tokyo, Japan.
Sato
K
K
Yamada
T
T
Maitani
T
T
D000595:Amino Acid Sequence; D002104:Cadmium / Q000494:pharmacology; D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid; D005978:Glutathione; D008667:Metalloproteins / Q000096:biosynthesis*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry*; D054811:Phytochelatins; D010940:Plant Proteins / Q000096:biosynthesis*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D010945:Plants, Edible / Q000187:drug effects / Q000378:metabolism*
D002614:Chelating Agents; D008667:Metalloproteins; D010455:Peptides; D010940:Plant Proteins; D002104:Cadmium; D054811:Phytochelatins; D005978:Glutathione