title: Epstein-Barr virus and gastric carcinoma in Western patients: comparison of pathological parameters and p53 expression in EBV-positive and negative tumours.
abstract: OBJECTIVE
  The presence of Epstein-Barr virus (EBV) was studied in 56 gastric carcinomas from Western patients by in-situ hybridization for EBV-encoded RNAs (EBER). EBV-positive and negative carcinomas were compared for various pathological parameters including p53 overexpression.
  
  
  RESULTS
  EBERs transcripts were detected in seven cases overall: four cases of 52 conventional carcinomas (7. 7%) and three cases of four gastric carcinomas with lymphoid stroma (75%). EBER positivity was diffuse in five cases and restricted to a localized area of the tumour in two cases of conventional carcinoma. A monoclonal EBV genomic pattern was demonstrated in the case tested by Southern blot analysis. By immunohistochemical analysis, neither EBV latent or lytic cycle proteins nor C3d/EBV receptor were expressed by neoplastic cells. EBER positivity was significantly correlated with prominent lymphoid reaction (P = 0.0002) which was associated with numerous PS100-positive dendritic cells and with HLA-DR expression by tumour cells (P = 0.03). p53 immunoreactivity in more than 30% of tumour cells was detected in 25 out 49 EBV-negative cases and was absent in EBV-positive cases except in one case with focal EBER-positivity.
  
  
  CONCLUSIONS
  Focal staining for EBER is an unusual finding in the setting of gastric carcinoma and these results suggest that there might be two types of EBV-associated gastric carcinoma in which the viral infection will play a different role. The presence of a stromal lymphoid reaction which is strongly correlated with EBV positivity, is associated with antigen-presenting ability by HLA-DR-positive tumour cells or abundant dendritic cells. The function of p53 appears preserved in all EBV-associated carcinomas except in one case with focal EBER expression whereas the immunohistochemical pattern of p53 is suggestive of a mutational phenomenon in 51% of EBV-negative cases.
authors.affiliation: Service d'Anatomie et de Cytologie Pathologiques, Hôpital Jean Verdier, Bondy, France.
authors.forename: F
authors.initials: F
authors.lastname: Chapel
authors.forename: B
authors.initials: B
authors.lastname: Fabiani
authors.forename: F
authors.initials: F
authors.lastname: Davi
authors.forename: M
authors.initials: M
authors.lastname: Raphael
authors.forename: M
authors.initials: M
authors.lastname: Tepper
authors.forename: G
authors.initials: G
authors.lastname: Champault
authors.forename: C
authors.initials: C
authors.lastname: Guettier
mesh_terms: D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology