title: Stereochemical control of DNA biosynthesis.
abstract: Stereochemical control of DNA biosynthesis was studied using several DNA-synthesizing complexes containing, in each case, a single substitution of a 2'-deoxy-D-nucleotide residue by an enantiomeric L-nucleotide residue in a DNA chain (either in the primer or in the template) as well as 2'-deoxy-L-ribonucleoside 5'-triphosphates (L-dNTPs) as substrates. Three template-dependent DNA polymerases were tested, Escherichia coli DNA polymerase I Klenow fragment, Thermus aquaticus DNA polymerase and avian myeloblastosis virus reverse transcriptase, as well as template-independent calf-thymus terminal deoxynucleotidyl transferase. Very stringent control of stereoselectivity was demonstrated for template-dependent DNA polymerases, whereas terminal deoxynucleotidyl transferase was less selective. DNA polymerase I and reverse transcriptase catalyzed formation of dinucleoside 5',5'-tetraphosphates when L-dTTP was used as substrate. Comparison between models of template-primer complexes, modified or not by a single L-nucleotide residue, revealed striking differences in their geometry.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Str., Moscow 117984, Russia,
authors.forename: V V
authors.initials: VV
authors.lastname: Sosunov
authors.forename: F
authors.initials: F
authors.lastname: Santamaria
authors.forename: L S
authors.initials: LS
authors.lastname: Victorova
authors.forename: G
authors.initials: G
authors.lastname: Gosselin
authors.forename: B
authors.initials: B
authors.lastname: Rayner
authors.forename: A A
authors.initials: AA
authors.lastname: Krayevsky
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D004261:DNA Replication; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA