Human milk fractions inhibit the adherence of diffusely adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) to HeLa cells.
Binding to a specific receptor is an essential step for most enteropathogens to initiate an intestinal infection. We analyzed the inhibitory effect of human milk and its protein components on adhesion of two diarrheagenic Escherichia coli strains, diffusely adherent E. coli (DAEC) and enteroaggregative E. coli (EAEC), to HeLa cells. Defatted milk, whey proteins, immunoglobulin and non-immunoglobulin fractions, in concentrations lower than usually found in whole milk, inhibited both DAEC and EAEC adhesion, indicating that human milk components may contribute to the defense of the infants against enteropathogens.
Nascimento de Araújo
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Laboratório de Microbiologia, Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, 70910-900, Brasília, Brazil.
Giugliano
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LG
D001422:Bacterial Adhesion / Q000187:drug effects*; D005591:Chemical Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000494:pharmacology; D007768:Lactalbumin / Q000494:pharmacology; D007781:Lactoferrin / Q000494:pharmacology; D008894:Milk Proteins / Q000494:pharmacology; D008895:Milk, Human / Q000737:chemistry*; D012709:Serum Albumin / Q000494:pharmacology; D000067816:Whey Proteins
D007143:Immunoglobulin Heavy Chains; D008894:Milk Proteins; D012709:Serum Albumin; D000067816:Whey Proteins; D007768:Lactalbumin; D007781:Lactoferrin