title: Secondary structural and phylogenetic implications of nuclear large subunit ribosomal RNA in the ectomycorrhizal fungus Tricholoma matsutake.
abstract: The sequence of large subunit (LSU) and 5.8S rRNA genes has been determined for Tricholoma matsutake. A secondary structure model was predicted for both LSU and 5.8S rRNAs, showing most of the structural features consistent with those of the consensus secondary structure model proposed for the eukaryotic cytoplasmic LSU rRNAs. With a reconstructed eukaryotic phylogeny based on full-length LSU rDNA sequences, T. matsutake was placed on the same branch with Cryptococcus neoformans as its closest neighbor. We proposed that T. matsutake be considered as one of the representative members of the division Basidiomycota. Here we report for the first time the complete LSU rRNA gene sequence in T. matsutake, a member of Homobasidiomycetes.
authors.affiliation: Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Korea.
authors.forename: S K
authors.initials: SK
authors.lastname: Hwang
authors.forename: J G
authors.initials: JG
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics*; D004271:DNA, Fungal / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D005800:Genes, Fungal; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012331:RNA, Fungal / Q000737:chemistry*; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012340:RNA, Ribosomal, 5.8S