Evaluation of an automated system for identification of Enterobacteriaceae and nonfermenting bacilli.
The performance of the Vitek 2 (bioMérieux, France), a new fully automated system allowing rapid identification of microorganisms and susceptibility testing, and the Vitek 2 ID-GNB card (bioMérieux) was evaluated using 502 clinical isolates and stock collection strains of gram-negative rods belonging to 70 taxa. The number of isolates correctly identified to species and genus levels was 430 (85.7%) and 485 (96.6%), respectively. Clinical isolates of both Enterobacteriaceae and non-Enterobacteriaceae were better identified at the species level (95.3% and 74%, respectively) than stock collection strains (86.4% and 52.2%, respectively). The Vitek 2 ID-GNB card provides after 3 h a highly acceptable level of accuracy for identification of Enterobacteriaceae and non-Enterobacteriaceae, including most atypical strains encountered in clinical situations.
Jossart
M F
MF
Centre Hospitalier Régional Universitaire, Laboratoire de Bactériologie-Hygiène, Lille, France.
Courcol
R J
RJ
D001331:Automation; D015373:Bacterial Typing Techniques; D004755:Enterobacteriaceae / Q000145:classification* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic