title: Optimization of isoamyl acetate production by using immobilized lipase from Mucor miehei by response surface methodology.
abstract: Immobilized lipase from Mucor miehei was employed for the esterification of isoamyl alcohol with acetic acid in n-heptane solvent. The important process variables studied were enzyme/substrate (E/S) ratio, alcohol (acid) concentration, and incubation period. Based on Box-Behnken design of experiments, a second order response function was developed. The percentage esterification increased with both E/S ratio and time and decreased with alcohol (acid) concentration. The model indicated optimum conditions for maximum esterification ranging from 20 to 99.6% in the alcohol (acid) concentration range of 0.031 to 0.3 M for a range of E/S ratios 8.33 to 50 g/mol, which were in good agreement with the experimental yields.
authors.affiliation: Fermentation Technology & Bio-Engineering Department, Central Food Technological Research Institute, Mysore, India
authors.lastname: Hari Krishna S
authors.initials: B
authors.lastname: Manohar
authors.initials: S
authors.lastname: Divakar
authors.initials: SG
authors.lastname: Prapulla
authors.initials: NG
authors.lastname: Karanth