title: Antibody against Testudo herpesvirus is not common in Chinese soft-shelled turtles.
abstract: Seventy-six serum samples of Chinese soft-shelled turtles (Trionyx sinensis) were collected at Jiangsu Province, China. The neutralization test (NT) was performed with the sera, Testudo herpesvirus (THV) and turtle heart cells (THC). Neutralizing antibodies were detected in five of 76 samples and the titres were 1:10-1:20. Having optimized the conditions, the dot-enzyme-linked immunosorbent assay (Dot-ELISA) was developed and eight serum samples exhibited positive results. Five samples were positive by both NT and Dot-ELISA. The percentage of positive samples was only 6.6% (NT) and 11% (ELISA). It is suggested that THV infection is not a serious problem for the Chinese soft-shelled turtle culture in this region.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, China.
authors.forename: Y C
authors.initials: YC
authors.lastname: Nie
authors.forename: C P
authors.initials: CP
authors.lastname: Lu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D002478:Cells, Cultured; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006564:Herpesviridae / Q000276:immunology* / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D005779:Immunodiffusion / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D014426:Turtles
chemical_list: D000914:Antibodies, Viral