title: Purification and partial characterization of a second cysteine proteinase inhibitor from ungerminated barley (Hordeum vulgare L.).
abstract: It was previously shown that ungerminated barley contains inhibitors that suppress the activities of green malt cysteine proteinases. This paper reports the purification and partial characterization of a second barley cysteine endoproteinase inhibitor, a protein called lipid transfer protein 2 (LTP2). The chromatographically purified inhibitor had a molecular mass of 7112. The amino acid composition and sequence data of the purified inhibitor indicated that it was a protein whose gene, but not the protein itself, was isolated earlier from barley aleurone tissue. The purified protein inhibited the activities of electrophoretically separated green malt cysteine proteinases but not the activities of the serine- or metalloproteinases. The purified LTP2 inhibited the same proteases as the LTP1 that was characterized previously but was present in the mature seed in much smaller amounts. Neither LTP1 nor LTP2 has been proven to transport lipids in vivo, and it seems possible that both serve to keep cysteine endoproteinases that are synthesized during barley seed development inactive until the plant needs them. The small amount of LTP2 in the seed made it impossible to determine whether it, like LTP1, is involved in beer foam formation. Because of its proteinase-inhibiting ability and its resistance to heat inactivation, some of the LTP2 may persist in beer.
authors.affiliation: Cereal Crops Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Madison, Wisconsin 53705, USA. bljones@facstaff.wisc.edu
authors.forename: B L
authors.initials: BL
authors.lastname: Jones
authors.forename: L A
authors.initials: LA
authors.lastname: Marinac
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D050556:Fatty Acid-Binding Proteins; D018525:Germination; D001467:Hordeum / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification*
chemical_list: D002352:Carrier Proteins; D015853:Cysteine Proteinase Inhibitors; D050556:Fatty Acid-Binding Proteins; C109356:LTP2 protein, plant; C422456:Ltp1 protein, barley; D010940:Plant Proteins