Redox- and pH-dependent association of plastocyanin with lipid bilayers: effect on protein conformation and thermal stability.
The effect of electrostatic interactions on the conformation and thermal stability of plastocyanin (Pc) was studied by infrared spectroscopy. Association of any of the two redox states of the protein with positively charged membranes at neutral pH does not significantly change the secondary structure of Pc. However, upon membrane binding, the denaturation temperature decreases, regardless of the protein redox state. The extent of destabilization depends on the proportion of positively charged lipid headgroups in the membrane, becoming greater as the surface density of basic phospholipids increases. In contrast, at pH 4.8 the membrane binding-dependent conformational change becomes redox-sensitive. While the secondary structures and thermal stabilities of free and membrane-bound oxidized Pc are similar under acidic conditions, the conformation of the reduced form of the protein drastically rearranges upon membrane association. This rearrangement does not depend on electrostatic interactions to occur, since it is also observed in the presence of uncharged lipid bilayers. The conformational transition, only observed for reduced Pc, involves the exposure of hydrophobic regions that leads to intermolecular interactions at the membrane surface. Membrane-mediated partial unfolding of reduced Pc can be reversed by readjusting the pH to neutrality, in the absence of electrostatic interactions. This redox-dependent behavior might reflect specific structural requirements for the interaction of Pc with its redox partners.
Taneva
S G
SG
Unidad de Biofísica (CSIC - UPV/EHU), and Departamento de Bioquímica y Biología Molecular, Universidad del País Vasco, Aptdo. 644, 48080, Bilbao, Spain.
Donchev
A A
AA
Dimitrov
M I
MI
Muga
A
A
D004355:Drug Stability; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005456:Fluorescent Dyes; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry*; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines / Q000737:chemistry; D018515:Plant Leaves; D010970:Plastocyanin / Q000737:chemistry*; D011487:Protein Conformation; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D055672:Static Electricity; D013816:Thermodynamics; D014197:Trees
D005229:Fatty Acids, Monounsaturated; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; D010970:Plastocyanin; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane