title: Low-doses of ionising radiation induce melanoma metastases and trigger the immune system--adrenal axis feedback loop.
abstract: Low-doses of ionising radiation are frequently implicated in triggering and/or accelerating the growth of skin and other malignancies. It seemed probable that the radiation at similar dose levels might initiate metastasis from already existing tumours. Highly pigmented human melanoma xenograft that had lost its ability for a spontaneous metastasising and grown subcutaneously in athymic mice was exposed to very low and well-defined doses of ionising radiation to determine whether low linear energy transfer radiation can restore metastatic potential of the tumour. To ensure that all effects derived from radiation-activated neoplastic cells only, I was delivered selectively to the cutaneous melanoma instead of using the external beam. The direct response of these tumours to radiation was monitored by determining the growth rate of the lesions. Histopathological methods were employed to detect metastases. The lowest radiation dose of approximately 6 cGy deposited in the tumours initiated metastatic spread in all animals. Gradual increase of the radiation doses diminished both the frequency of the appearance of metastases and their distance from the primary lesions. There were no metastases from non-irradiated melanomas. The highest dose used (60 cGy) did not affect significantly the growth of cutaneous (primary) tumours, but lower doses that enhanced inflammatory infiltration of the lesions reduced tumour growth. Such radiation-stimulated immune responses were accompanied by increased pigmentation in cutaneous lesions and activation of the adrenal cortex indicating that the immune system-adrenal axis feedback loop had been triggered. The results demonstrate that very low-doses of ionising radiation induce melanoma metastases. The phenomenon is accompanied by the stimulation of the immune system-adrenal axis feedback loop that regulates eicosanoid synthesis, thereby suggesting an involvement of these molecules in the process. Radiation doses approaching the therapeutic level do not initiate melanoma dissemination.
authors.affiliation: Department of Molecular Pathology, University College London Medical School, Windeyer Institute, U.K. e.link@ucl.ac.uk
authors.forename: E M
authors.initials: EM
authors.lastname: Link
authors.forename: K
authors.initials: K
authors.lastname: Flanagan
authors.forename: A S
authors.initials: AS
authors.lastname: Michalowski
authors.forename: P J
authors.initials: PJ
authors.lastname: Blower
mesh_terms: D000311:Adrenal Glands / Q000276:immunology; D000818:Animals; D005246:Feedback / Q000528:radiation effects; D005260:Female; D006801:Humans; D007107:Immune System / Q000528:radiation effects*; D008545:Melanoma / Q000276:immunology / Q000556:secondary*; D051379:Mice; D008819:Mice, Nude; D011839:Radiation, Ionizing; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology*