Differential expression of small heat shock protein 27 in the rat hippocampus and septum after fimbria-fornix lesion.
mRNA, Western analysis and immunohistochemistry were used to study the expression of the small heat shock protein (HSP) 27 in the rat septum and hippocampus following fimbria-fornix lesions, a model of neurodegeneration and regeneration. (HSP) 27 mRNA level was increased 2.5-fold in the medial septum 3 days after lesion and this increase persisted for 10 days. In the hippocampus, after an initial 15-fold increase at 3 days post-injury, HSP27 mRNA returned to basal levels 10 days after the lesion. Three and 10 days after lesion, HSP27 protein levels were increased in the septum (4.5 and 5-fold, respectively) and hippocampus (65 and 10-fold, respectively). The morphology of the HSP27 positive cells was indistinguishable from that of GFAP-immunoreactive cells. In addition, in the septum of injured rats, occasional neurons were heavily labelled with anti-HSP27 antibodies. Thus, up-regulation of HSP27, particularly in glial cells, may be a component of glial input in the processes on degeneration/regeneration which occur in this model.
Anguelova
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Sanofi-Synthélabo, CNS Research Departement, 31, Avenue Paul - Vaillant Couturier, BP 110 92225 Bagneux, Cedex, France. eanguelo@bagneux.synhtelabo.fr
Smirnova
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D000818:Animals; D015153:Blotting, Western; D020712:Fornix, Brain / Q000473:pathology*; D005786:Gene Expression Regulation; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020665:Septum of Brain / Q000737:chemistry / Q000378:metabolism*
D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins; D012333:RNA, Messenger