A peptide from the adenovirus fiber shaft forms amyloid-type fibrils.
The fiber protein of adenovirus consists of a C-terminal globular head, a shaft and a short N-terminal tail. The crystal structure of a stable domain comprising the head plus a part of the shaft of human adenovirus type 2 fiber has recently been solved at 2.4 A resolution [van Raaij et al. (1999) Nature 401, 935-938]. A peptide corresponding to the portion of the shaft immediately adjacent to the head (residues 355-396) has been synthesized chemically. The peptide failed to assemble correctly and instead formed amyloid-type fibrils as assessed by electron microscopy, Congo red binding and X-ray diffraction. Peptides corresponding to the fiber shaft could provide a model system to study mechanisms of amyloid fibril formation.
Luckey
M
M
Department of Chemistry, San Francisco State University, 1600 Holloway avenue, 94132, San Francisco, CA, USA.
Hernandez
J
J
Arlaud
G
G
Forsyth
V T
VT
Ruigrok
R W
RW
Mitraki
A
A
D000260:Adenoviruses, Human / Q000737:chemistry*; D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D001718:Birefringence; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins; D004396:Coloring Agents / Q000378:metabolism; D003224:Congo Red / Q000378:metabolism; D008854:Microscopy, Electron; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D014961:X-Ray Diffraction
D000682:Amyloid; D036022:Capsid Proteins; D004396:Coloring Agents; D010446:Peptide Fragments; C018315:hexon capsid protein, Adenovirus; D003224:Congo Red