title: Sarcina -like bacteria, Clostridium fallax and Clostridium sordellii in lambs with abomasal bloat, haemorrhage and ulcers.
abstract: A study of abomasal disease in lambs aged 2-5 weeks, made during the period 1993-1998, included 67 cases and 45 non-affected controls. Gross pathological findings included various combinations of bloat, haemorrhage and ulcers in the abomasum. Sarcina -like bacteria were found in sections and smears from the abomasum of 79% (53/67) of the cases. From one case, a lamb with abomasal bloat, the anaerobic "packet"-forming Sarcina ventriculi was cultivated from the abomasal contents and identified by biochemical reactions and sequencing of the 16S rRNA gene. Sarcina -like bacteria were observed microscopically in specimens from 94% (44/47) of the lambs with abomasal gas and in 45% (9/20) of those with ulcers or haemorrhage or both but little gas. On culture, abomasal contents from 41 cases yielded Clostridium fallax from 16 (39%) and Clostridium sordellii from eight (20%); abomasal cultures from 30 control lambs were negative for the three bacterial species. Quantitative cultivation, carried out on abomasal contents from live lambs and lambs dead </=3 h, showed that Clostridium perfringens, Escherichia coli and Lactobacillus spp. were present in the majority of both cases and controls, with no significant differences in the mean numbers.
authors.affiliation: Department of Sheep and Goat Research, The Norwegian School of Veterinary Science, Kyrkjevegen, Sandnes, 332/334,4325, Norway.
authors.forename: S
authors.initials: S
authors.lastname: Vatn
authors.forename: M A
authors.initials: MA
authors.lastname: Tranulis
authors.forename: M
authors.initials: M
authors.lastname: Hofshagen
mesh_terms: D000018:Abomasum / Q000382:microbiology* / Q000473:pathology; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D001431:Bacteriological Techniques; D003013:Clostridium / Q000302:isolation & purification; D003016:Clostridium perfringens / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D007778:Lactobacillus / Q000302:isolation & purification; D008297:Male; D008969:Molecular Sequence Data; D010437:Peptic Ulcer / Q000382:microbiology / Q000662:veterinary*; D010438:Peptic Ulcer Hemorrhage / Q000382:microbiology / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012505:Sarcina / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology* / Q000473:pathology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S