[p53 gene mutations in BALB/c 3T3 cells transformed by crocidolite].
This study sought to address the relationship between crocidolite and p53 gene mutation. The mutations of p53 gene in 8 BALB/c 3T3 cell lines transformed by crocidolite were analysed. Altogether 11 exons of the gene from 8 cell lines were detected by PCR-SSCP. 7 alterations were found; 2 of them were located in exon 4-6, and 5 in 9-11. Most of the mutations (5/7) were of one more band than that of wild cell from SSCP, and alterations were randomly scattered among the crocidolite doses groups. The results suggest that the presence of a p53 alteration is not related to the dose of crocidolite used. Besides, p53 mutation may occur in a relatively later period of the growth of the transformed cell lines. The results also showed that the mutations occurred predominantly in exons 9-11. This was different from that seen in human mesothelioma where mutations in the exon 5-8 of p53 gene were more frequently observed.
Lin
F
F
Toxicology Department, School of Public Health, Chengdu.
Zhai
C
C
Liu
Y
Y
Zhou
G
G
Sun
Z
Z
Li
S
S
D016475:3T3 Cells; D000818:Animals; D017638:Asbestos, Crocidolite / Q000633:toxicity*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D005091:Exons; D016158:Genes, p53; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
D017638:Asbestos, Crocidolite