[Successful 48-72 h cold storage of dog kidneys with HX-1 solution].
HX-1 solution was used in this study to determine its effects on long term kidney preservation. 20 female and male mongrel dogs were randomly divided into control (HC-WCU solution) and experimental (HX-1 solution) groups. Kidneys were flushed with HC-WCU or HX-1 solution and stored at 0.4 degree C for 48 or 72 h. After being preserved, the kidney was implanted in the right groin of the animal and anastomosed to the iliac vessles. This procedure was immediately followed by a contralateral nephrectomy. The experimental findings were: 1. The maximum serum creatinine levels were 642.60 +/- 158.60 mumol/L and 686.20 +/- 154.04 mumol/L respectively in HC-WCU solution subgroups for 48 or 72 h cold storage. But the maximum serum creatinine levels were 448.32 +/- 36.90 mumol/L and 524.60 +/- 109.38 mumol/L respectively in HX-1 solution subgroups. 2. 80% of the kidneys were viable after 48 h storage in HC-WCU kidney solution, but 100% viable when stored in HX-1 solution: 40% of the kidneys were viable after 72 h storage in HC-WCU solution, but 60% viable when stored in HX-1 solution. 3. Histologically the kidney of dogs that died of renal failure was damaged worse in HC-WCU solution group than that in HX-1 solution group. These results suggest that HX-1 solution is superior to HC-WCU solution for preserving kidneys and HX-1 solution may store up the kidney of dogs to 48-72 h.
Peng
B
B
Department of Surgery, First Affiliated Hospital, Chengdu.
Wu
H
H
Shen
W
W
Wang
X
X
Zhang
R
R
Wu
Y
Y
D000818:Animals; D003404:Creatinine / Q000097:blood; D004285:Dogs; D004365:Drugs, Chinese Herbal; D005260:Female; D006085:Graft Survival; D007668:Kidney; D016030:Kidney Transplantation; D008297:Male; D019188:Organ Preservation Solutions; D011897:Random Allocation; D013997:Time Factors
D004365:Drugs, Chinese Herbal; D019188:Organ Preservation Solutions; D003404:Creatinine