Peritoneal mesothelial cells produce complement factors and express CD59 that inhibits C5b-9-mediated cell lysis.
The CD59 membrane protein confers protection from C5b-9-mediated cell lysis. Because evidence exists for complement (C) activation and generation of C5b-9 in the peritoneal cavity during chronic peritoneal dialysis (CPD), we investigated, on mesothelial cell (MC) lines, the expression of CD59 and the production of C components. Four MC lines were obtained from children on CPD, and two from non uremic children. CD59 expression on MCs was investigated with anti-CD59 monoclonal antibody (mAb) and polyclonal goat immunoglobulin G (IgG). MC lines were positive for staining with anti-CD59 mAb. Western blotting analysis of MC membrane demonstrated a band with the same molecular weight as CD59. Incubation of MC with anti-CD59 mAb abrogated the protective effect of CD59 (100% cytotoxicity). C3, C4, and C6 were detected in the supernatants of MC; in non uremic MC supernatants, C5, C7, C8, and C9 were also detectable, and C4 concentration was tenfold higher. CD59 expression confers to MCs protection from C5b-9-mediated lysis. MCs produce C factors. These findings suggest that production of complement components and expression of CD59 on MCs could play a role both in peritoneal cavity infection (decreased complement production) and in peritoneal membrane damage (decreased CD59 expression and reduced remesothelialization owing to MC lysis).
Barbano
G
G
Nephrology Department, G. Gaslini Children's Hospital, Genoa, Italy.
Cappa
F
F
Prigione
I
I
Tedesco
F
F
Pausa
M
M
Gugliemino
R
R
Pistoia
V
V
Gusmano
R
R
Perfumo
F
F
D018957:CD59 Antigens / Q000032:analysis*; D002460:Cell Line; D002648:Child; D015938:Complement Membrane Attack Complex / Q000276:immunology*; D003165:Complement System Proteins / Q000032:analysis*; D004847:Epithelial Cells / Q000276:immunology; D006801:Humans; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000276:immunology*
D018957:CD59 Antigens; D015938:Complement Membrane Attack Complex; D003165:Complement System Proteins