A common variant of the angiotensinogen gene and the risk of coronary artery disease in a German population.
The thymidine to cytosine transition at position 704 in exon 2 of the angiotensinogen gene leads to the amino acid substitution of threonine for methionine (T235 variant) and is responsible for elevated plasma levels of angiotensinogen. To examine the influence of T235 on the risk of coronary artery disease (CAD) we genotyped 184 CAD patients, 77 controls in whom CAD was excluded angiographically, and 155 healthy controls without signs of CAD by polymerase chain amplification and restriction enzyme digestion. Allele frequencies for A (wildtype) and a (mutant allele) in the total study population were 0.538 and 0.462, 0.536 and 0.464 in the healthy controls, and 0.481 and 0.519 in patients with excluded CAD, respectively. The allele frequencies and the genotype distribution in these groups did not show a significant difference. In conclusion, we did not observe an association between the T235 variant of the angiotensinogen gene and the risk of CAD.
Reinhardt
D
D
Department of Internal Medicine, University of Jena, Germany. drei@polkim.med.uni-jena.de
Sigusch
H H
HH
Vogt
S F
SF
Zeiss
C
C
Farker
K
K
Hoffmann
A
A
Müller
S
S
D019943:Amino Acid Substitution / Q000235:genetics; D000808:Angiotensinogen / Q000235:genetics*; D017023:Coronary Angiography; D003327:Coronary Disease / Q000453:epidemiology* / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors
D000808:Angiotensinogen; D004247:DNA