Selenium determination in mother and child's hair by electrothermal atomic absorption spectrometry.
A method for the selenium determination in a mother and her child's hair using palladium as a chemical modifier was optimized. The sample was digested with nitric acid and hydrogen peroxide and diluted to 5 ml. To achieve complete mineralization the samples were ashed at 1200 degrees C in the presence of palladium as a chemical modifier. The optimum atomization temperature was 1900 degrees C. The precision and accuracy of the method were studied using the reference material CRM 397. Results of calibration using aqueous standards and the standard addition method were compared. The method was applied to the selenium determination in 30 samples of the mother's and child's hair. The levels found were 0.54 +/- 0.34 microgram/g for mother's hair and 0.77 +/- 0.25 microgram/g for child's hair.
Bermejo Barrera
P
P
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Spain. qn1956@usc.es
Lorenzo Alonso
M J
MJ
Bermejo Barrera
A
A
Cocho de Juan
J A
JA
Fraga Bermúdez
J M
JM
D000328:Adult; D002138:Calibration; D002648:Child; D006197:Hair / Q000737:chemistry*; D006801:Humans; D009035:Mothers; D010165:Palladium; D012044:Regression Analysis; D015203:Reproducibility of Results; D012643:Selenium / Q000032:analysis*; D012680:Sensitivity and Specificity; D013054:Spectrophotometry, Atomic / Q000379:methods
D010165:Palladium; D012643:Selenium