Functional association between the nef gene product and gag-pol region of HIV-1.
Nef gene function is diverse among virus isolates of primate immunodeficiency viruses. We found differential effects of nef mutation on the virus replication between two HIV-1 clones, NL432 and LAI. The nef mutation in NL432 affected the infectivity more severely compared with that in LAI, although the Nef functions of both clones were comparable. Analysis with a series of chimeric viruses between NL432 and LAI revealed that the gag-pol region was responsible for the differential effect of nef mutation. The functional association between Nef and gag-pol suggested that one of the potential targets of Nef was located within the gag-pol region.
Ono
T
T
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Japan.
Iwatani
Y
Y
Nishimura
A
A
Ishimoto
A
A
Sakai
H
H
D002460:Cell Line; D015685:Fusion Proteins, gag-pol / Q000235:genetics / Q000378:metabolism*; D015693:Gene Products, nef / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism* / Q000472:pathogenicity / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D011485:Protein Binding; D014771:Virion; D054311:nef Gene Products, Human Immunodeficiency Virus
D015685:Fusion Proteins, gag-pol; D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus