title: Effects of retroviral-mediated herpes simplex virus thymidine kinase gene transfer to murine neuroblastoma cell lines in vitro and in vivo.
abstract: Selective introduction of genes conferring chemosensitivity on proliferating tumor cells can be used to treat cancer. We investigated the efficacy of retrovirus-mediated gene transfer of the herpes simplex virus thymidine kinase (HSV-TK) gene to murine neuroblastoma cell lines (neuro-2a) in vitro and in vivo. Retrovirus-mediated HSV-TK gene transfer to the neuro-2a cells resulted in sensitivity to ganciclovir (GCV) in vitro. In A/J mice, tumors produced from HSV-TK transduced neuro-2a cells regressed after GCV treatment. Intratumoral injection of recombinant retrovirus expressing HSV-TK gene also inhibited growth of the tumor established in A/J mice. These results demonstrate that HSV-TK gene therapy might be a feasible approach for inhibiting the growth of neuroblastoma.
authors.affiliation: Department of Pediatrics, Kangdong Sacred-Heart Hospital, Hallym University College of Medicine, Kangdong-Ku, Seoul, South Korea. hscho@www.hallym.or.kr
authors.forename: H S
authors.initials: HS
authors.lastname: Cho
authors.forename: Y N
authors.initials: YN
authors.lastname: Park
authors.forename: C J
authors.initials: CJ
authors.lastname: Lyu
authors.forename: C H
authors.initials: CH
authors.lastname: Yang
authors.forename: K Y
authors.initials: KY
authors.lastname: Ryoo
authors.forename: B S
authors.initials: BS
authors.lastname: Kim
authors.forename: K Y
authors.initials: KY
authors.lastname: Kim
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000818:Animals; D005260:Female; D015774:Ganciclovir / Q000627:therapeutic use; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D020287:In Situ Nick-End Labeling; D051379:Mice; D008815:Mice, Inbred Strains; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D012190:Retroviridae; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000473:pathology
chemical_list: D013937:Thymidine Kinase; D015774:Ganciclovir