title: The molar vesicle fluid contains the beta-core fragment of human chorionic gonadotropin.
abstract: The human chorionic gonadotropin (hCG) beta-core fragment (beta-CF) is a major molecular form of hCG beta subunit (hCGbeta) immunoreactivity in the urine of pregnant women and patients with trophoblastic disease. The majority of evidence supports the fact that the beta-CF is a degradative product of intact hCG and free hCGbeta in the kidneys. We found a beta-CF-like substance in the fluid of molar vesicles from a patient with complete hydatidiform mole. The molar fluid beta-CF (mbeta-CF) was indistinguishable from the beta-CF in the patient's urine (ubeta-CF) by immunoreactivity and by elution profile on gel chromatography. The binding study to lectins, however, showed that mbeta-CF contains a carbohydrate moiety that differs from that of ubeta-CF. Immunohistochemistry with anti-beta-CF antibody demonstrated a strong immunoreactivity in a large number of macrophages in the molar villous stroma. In vitro incubation of intact hCG with peritoneal macrophages showed a slow increase of intact hCG in the cell cytosol with the appearance of beta-CF-like substance in the cell supernatant. In conclusion, the source of beta-CF in molar fluid is likely to be macrophages existing in the villous stroma. Thus macrophages may ingest intact hCG and act as a local regulator of gonadotropic hormones.
authors.affiliation: Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Honjo 1-1-1, Kumamoto-City, 860-8556, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: H
authors.initials: H
authors.lastname: Katabuchi
authors.forename: M
authors.initials: M
authors.lastname: Araki
authors.forename: T
authors.initials: T
authors.lastname: Ohba
authors.forename: T
authors.initials: T
authors.lastname: Koizumi
authors.forename: H
authors.initials: H
authors.lastname: Okamura
authors.forename: R
authors.initials: R
authors.lastname: Nishimura
mesh_terms: D001826:Body Fluids / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000097:blood / Q000378:metabolism / Q000652:urine; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000378:metabolism; D010446:Peptide Fragments / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; C063554:urinary gonadotropin fragment