title: [Isolation of antibacterial polypeptides of human cervical mucus].
abstract: The acid-soluble extract of human cervical mucus was obtained by solving mucus with 5% acetic acid in the presence of protease inhibitor. The antibacterial activity of the acid-soluble extract was analyzed by gel overlay technique. The result showed that two protein bands which were designated human cervical protein-1 (Hcp-1), human cervical protein-2(Hcp-2) were potently antibacterial against E. coli 25922 and S. aureus 25923. Tricine-SDS-PAGE analysis indicated that Hcp-1, Hcp-2 actually contained three and four protein bands respectively. The molecular weights of Hcp-1 were 6.7 kd, 10 kd, 15.4 kd; these of Hcp-2 were 4.4 kd, 6.7 kd, 9 kd, 15.4 kd. Our studies suggested that human cervical mucosa might secrete some currently-unknown antibacterial polypeptides which play an important role in the cervical defense against infection.
authors.affiliation: Department of Pathophsiology, School of Basic Medical Sciences, Chengdu.
authors.forename: N
authors.initials: N
authors.lastname: Huang
authors.forename: X
authors.initials: X
authors.lastname: Pan
authors.forename: Q
authors.initials: Q
authors.lastname: Wu
authors.forename: B
authors.initials: B
authors.lastname: Wang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D002582:Cervix Mucus / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides