title: Simple and efficient method for the detection of diethylenetriaminepentaacetic acid.
abstract: Diethylenetriaminepentaacetic acid (DTPA) is a commonly used chelating agent. Its antiviral, antibacterial and immunomodulatory effects are well documented. DTPA forms a highly stable complex with lead (II) with an increased absorption coefficient and a bathochromic shift of the absorption maximum compared to pure DTPA. Based on this complex a high-performance liquid chromatographic method for the quantitative detection of DTPA in biological fluids was developed. A calibration curve was prepared and linearity was shown in the concentration range between 10 mg l(-1) and 1000 mg l(-1) DTPA. The recovery in water and in human plasma showed the method to be suitable for routine use.
authors.affiliation: Institut für Pharmazeutische Technologie, Biozentrum Niederursel, Johann Wolfgang Goethe-Universität, Frankfurt a.M., Germany.
authors.forename: C
authors.initials: C
authors.lastname: Weber
authors.forename: M
authors.initials: M
authors.lastname: Michaelis
authors.forename: J U
authors.initials: JU
authors.lastname: Vogel
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: J
authors.initials: J
authors.lastname: Kreuter
authors.forename: K
authors.initials: K
authors.lastname: Langer
mesh_terms: D000327:Adsorption; D002614:Chelating Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007854:Lead / Q000378:metabolism; D004369:Pentetic Acid / Q000032:analysis* / Q000097:blood; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D014867:Water / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D014867:Water; D007854:Lead; D004369:Pentetic Acid