Effects of newly synthesized analogs of MIF-1 containing unnatural amino acids on electrically evoked smooth muscle contractions.
New MIF-1 (Pro-Leu-Gly-NH2) analogs containing unnatural amino acids such as L-canavanine (Cav) and L-cysteic acid S-(2-aminoethyl)amide (sLys) have been synthesized and in vitro experiments were performed to study their action on neurotransmission in target tissues with adrenergic and cholinergic neurotransmission. The experiments were carried out on electrically stimulated proximal guinea pig ileum (GPI) and the prostatic part of rat and rabbit vasa deferentia (VDR, VDRabb). The present results show that the newly synthesized Cav2-MIF and sLys2-MIF might affect electrically evoked smooth muscle contractions.
Pajpanova
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Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria. tamara@obzor.bio21.bas.bg
Bocheva
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Golovinsky
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D018663:Adrenergic Agents / Q000494:pharmacology*; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D002172:Canavanine / Q000737:chemistry; D018679:Cholinergic Agonists / Q000494:pharmacology*; D003544:Cysteic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D006168:Guinea Pigs; D007082:Ileum; D066298:In Vitro Techniques; D009075:MSH Release-Inhibiting Hormone / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D018377:Neurotransmitter Agents / Q000502:physiology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013564:Sympathetic Nervous System / Q000187:drug effects*; D014649:Vas Deferens / Q000187:drug effects
D018663:Adrenergic Agents; D000596:Amino Acids; D018679:Cholinergic Agonists; D018377:Neurotransmitter Agents; D002172:Canavanine; D009075:MSH Release-Inhibiting Hormone; D003544:Cysteic Acid