Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli.
Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 microgram of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.
Langermann
S
S
MedImmune, Inc., Gaithersburg, MD 20878, USA. langermanns@medimmune. com
Möllby
R
R
Burlein
J E
JE
Palaszynski
S R
SR
Auguste
C G
CG
DeFusco
A
A
Strouse
R
R
Schenerman
M A
MA
Hultgren
S J
SJ
Pinkner
J S
JS
Winberg
J
J
Guldevall
L
L
Söderhäll
M
M
Ishikawa
K
K
Normark
S
S
Koenig
S
S
D018829:Adhesins, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D018830:Adhesins, Escherichia coli; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004926:Escherichia coli / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control*; D005243:Feces / Q000382:microbiology; D036781:Fimbriae Proteins; D006801:Humans; D008252:Macaca fascicularis; D013270:Stomach / Q000382:microbiology; D001743:Urinary Bladder / Q000382:microbiology; D014552:Urinary Tract Infections / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; C058769:fimH protein, E coli; D036781:Fimbriae Proteins