Role of T cell subset on the immunosuppression induced by Actinobacillus actinomycetemcomitans.
Purified splenic T cells from C3H/HeN mice primed with sonic extract (SE) from Actinobacillus actinomycetemcomitans were adoptively transferred to syngeneic mice with sheep red blood cell (SRBC). The transfer of splenic T cells from mice, primed with SE for 8 days, resulted in the dose-dependent inhibition of IgM anti-SRBC plaque forming cells (PFC) compared with normal and BSA-primed splenic T cells. Furthermore, the transfer of cells from mice primed with 200 micrograms of SE for 8 days to syngeneic mice caused the highest inhibition. Immunosuppression did not depend on the B cell population in spleen from donor mice primed with SE. Splenic T cells from SE-treated mice could suppress the T cell-dependent proliferative responses of co-cultured normal spleen cells in vitro. Analysis of T cell subsets of spleen cells from mice treated with immunosuppressive factor (ISF) showed that the suppressor cell is susceptible to treatment with anti-CD8 and complement (C). SE-sensitized suppressor T cells also suppressed the secondary IgG anti-SRBC-PFC response after immunization with SRBC in vivo depending on sensitized periods induced by ISF. Treatment of T cells from mice which primed with ISF for 8 days, with goat anti-mouse CD8 antibody and C abrogated their suppressive effects, and secondary IgG response occurred. These results indicate that the adoptive transfer of SE-induced T cells, which increased suppressor function, caused the perfect blocking of the immunoresponse, allowing promotion of secondary infection.
Ochiai
K
K
Department of Microbiology, Nihon University School of Dentistry, Chiba, Japan.
Kurita-Ochiai
T
T
D019264:Adoptive Transfer; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D004912:Erythrocytes; D005260:Female; D006041:Goats; D007108:Immune Tolerance / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D012756:Sheep; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D013997:Time Factors; D014185:Transplantation, Isogeneic
D016827:CD8 Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins