title: [A rapid simple and reliable method for sequencing the targeted gene of shuttle vector pSP189].
abstract: The authors established a simple, rapid and effective procedures for sequencing the targeted a-gene SupF TRNA of shuttle vector pSP189, which is very important and useful in molecular mechanism research of mutagens. In this method, double-stranded plasmid DNA as template, prepared single-stranded DNA with alkali denaturation, applied r-32P-ATP for labelling 5'-end of primer, were used. A satisfactory result was achieved.
authors.affiliation: Institute of Food Safety Control and Inspection, Chinese Academy of Preventive Medicine, Beijing.
authors.forename: X
authors.initials: X
authors.lastname: Tan
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Luo
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D009152:Mutagenicity Tests; D017354:Point Mutation; D017422:Sequence Analysis, DNA