title: Monoclonal antibodies recognizing CD5, CD10 and CD23 in formalin-fixed, paraffin-embedded tissue: production and assessment of their value in the diagnosis of small B-cell lymphoma.
abstract: OBJECTIVE
  Assessment of the expression of antigens CD5, CD10 and CD23 can be of value in the differential diagnosis of small B-cell lymphoma. Correct subclassification is important since optimal treatment regimes differ between the subtypes. The aim of this study was to generate monoclonal antibodies recognizing these antigens in paraffin-embedded tissue and to assess their efficacy using a panel of cases of small B-cell lymphoma of various subtypes.
  
  
  RESULTS
  For each antibody synthetic recombinant protein and conventional murine hybridoma technology was employed. Monoclonal antibodies effective in formalin-fixed, paraffin-embedded tissue were successfully generated, designated NCL-CD5-4C7, NCL-CD10-270 and NCL-CD23-1B12, respectively. A series of 58 cases of small B-cell lymphoma including examples of each subtype (lymphocytic, follicle centre cell, mantle cell, marginal zone and lymphoplasmacytoid) was assembled and immunostaining for the respective antigens carried out using the monoclonal antibodies produced. Our results indicate that the antibodies are specific for their respective antigens and give the predicted phenotypic profile in the small B-cell lymphoma subtypes.
  
  
  CONCLUSIONS
  These novel monoclonal antibodies may be of value in routine diagnostic practice.
authors.affiliation: Department of Pathology and Haematology, The University of Newcastle upon Tyne, and Novocastra Laboratories Ltd., Newcastle-upon-Tyne, UK.
authors.forename: P
authors.initials: P
authors.lastname: Watson
authors.forename: K M
authors.initials: KM
authors.lastname: Wood
authors.forename: A
authors.initials: A
authors.lastname: Lodge
authors.forename: G G
authors.initials: GG
authors.lastname: McIntosh
authors.forename: I
authors.initials: I
authors.lastname: Milton
authors.forename: N H
authors.initials: NH
authors.lastname: Piggott
authors.forename: S J
authors.initials: SJ
authors.lastname: Proctor
authors.forename: P R
authors.initials: PR
authors.lastname: Taylor
authors.forename: S
authors.initials: S
authors.lastname: Smith
authors.forename: F
authors.initials: F
authors.lastname: Jack
authors.forename: H
authors.initials: H
authors.lastname: Bell
authors.forename: M
authors.initials: M
authors.lastname: Steward
authors.forename: J J
authors.initials: JJ
authors.lastname: Anderson
authors.forename: C H
authors.initials: CH
authors.lastname: Horne
authors.forename: B
authors.initials: B
authors.lastname: Angus
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D015153:Blotting, Western; D018956:CD5 Antigens / Q000032:analysis / Q000276:immunology; D019938:Cyclin D1 / Q000032:analysis / Q000276:immunology; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis / Q000378:metabolism; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000378:metabolism; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis / Q000378:metabolism; D051379:Mice; D015260:Neprilysin / Q000032:analysis / Q000276:immunology; D016612:Paraffin Embedding; D017455:Receptors, IgE / Q000032:analysis / Q000276:immunology; D016707:Tissue Fixation
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018956:CD5 Antigens; D005404:Fixatives; D017455:Receptors, IgE; D019938:Cyclin D1; D005557:Formaldehyde; D015260:Neprilysin