Direct single-step surface plasmon resonance analysis of interactions between small peptides and immobilized monoclonal antibodies.
Surface plasmon resonance (SPR) methods have been optimized to permit direct kinetic analysis of the antigenic peptide analytes interacting with immobilized monoclonal antibodies (mAbs). High reproducibility and a significant correlation between SPR and previous ELISA data on the same set of antibodies and peptides were observed. The kinetic data obtained provide further insight into the structure of the main antigenic site of foot-and-mouth disease virus (FMDV).
Gomes
P
P
Department of Organic Chemistry, University of Barcelona, Martí i Franquès 1-E-08028, Barcelona, Spain.
Giralt
E
E
Andreu
D
D
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D008969:Molecular Sequence Data; D015203:Reproducibility of Results; D020349:Surface Plasmon Resonance
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral