Deficiency of a STE20/PAK family kinase LOK leads to the acceleration of LFA-1 clustering and cell adhesion of activated lymphocytes.
Lymphocyte-oriented kinase (LOK) is a member of the STE20/p21-activated kinase (PAK) family and expressed predominantly in lymphoid organs. Generation of LOK-deficient mice revealed that the leukocyte-function-associated antigen (LFA-1)/intercellular adhesion molecules (ICAM)-mediated aggregation of mitogen-stimulated T cells was greatly enhanced in the absence of LOK. Though levels of total LFA-1 and ICAMs as well as the active form of LFA-1 on T cell blasts were comparable in the presence and absence of LOK, clustering of active LFA-1 detected by binding of soluble ICAM-1 was accelerated in the absence of LOK. These results suggest that LOK is potentially involved in the regulation of LFA-1-mediated lymphocyte adhesion.
Endo
J
J
Department of Immunology, The Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo, Japan.
Toyama-Sorimachi
N
N
Taya
C
C
Kuramochi-Miyagawa
S
S
Nagata
K
K
Kuida
K
K
Takashi
T
T
Yonekawa
H
H
Yoshizawa
Y
Y
Miyasaka
N
N
Karasuyama
H
H
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003208:Concanavalin A; D015698:Genomic Library; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*
D016169:Lymphocyte Function-Associated Antigen-1; D003208:Concanavalin A; D018799:Intercellular Adhesion Molecule-1; D017346:Protein Serine-Threonine Kinases; C108145:Stk10 protein, mouse