title: Structural studies of the Maillard reaction products of a protein using ion trap mass spectrometry.
abstract: The early stage products of the Maillard reaction of egg white lysozyme with D-glucose were studied. Incubation with D-glucose at 50 degrees C for 20 days caused reaction on the Lys and Arg residues of lysozyme as follows: all of the six Lys residues and 10 of the 11 Arg residues in lysozyme reacted with D-glucose; Arg 61 did not react with D-glucose. The Lys residues reacted with D-glucose with 1 mol of dehydration per mole of residue, and the Arg residues reacted with 2 mol of dehydration per mole of residue. The major constituent of the Amadori product with the epsilon-amino group of the Lys residue and the D-glucose was found to be the beta-pyranose form. The structure of the early stage product of the Maillard reaction of a protein with a sugar is the same as that of an amino acid with a sugar.
authors.affiliation: Central Research Laboratories, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi 210-8681, Japan.
authors.forename: U
authors.initials: U
authors.lastname: Tagami
authors.forename: S
authors.initials: S
authors.lastname: Akashi
authors.forename: T
authors.initials: T
authors.lastname: Mizukoshi
authors.forename: E
authors.initials: E
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Hirayama
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015416:Maillard Reaction; D013058:Mass Spectrometry; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000596:Amino Acids; D006023:Glycoproteins; D010455:Peptides; D011506:Proteins; D009113:Muramidase; D002918:Chymotrypsin