Binding of bovine papillomavirus type 4 E8 to ductin (16K proteolipid), down-regulation of gap junction intercellular communication and full cell transformation are independent events.
The E8 open reading frame of bovine papillomavirus type 4 encodes a small hydrophobic polypeptide that contributes to primary cell transformation by conferring to cells the ability to form foci and to grow in low serum and in suspension. Wild-type E8 binds in vitro to ductin, a component of gap junctions, and this binding is accompanied by a loss of gap junction intercellular communication in transformed bovine fibroblasts. However, through the analysis of a panel of E8 mutants, we show here that binding of E8 to ductin is not sufficient for down-regulation of gap junction communication and that there is no absolute correlation between down-regulation of gap junction communication and the transformed phenotype.
Ashrafi
G H
GH
Papillomavirus Group and Cell Communication Group, The Beatson Institute for Cancer Research, Glasgow, UK.
Pitts
J D
JD
Faccini
A
A
McLean
P
P
O'Brien
V
V
Finbow
M E
ME
Campo
S
S
D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D002417:Cattle; D002450:Cell Communication; D002460:Cell Line; D002472:Cell Transformation, Viral; D015536:Down-Regulation; D017629:Gap Junctions / Q000502:physiology* / Q000821:virology*; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames; D010641:Phenotype; D011485:Protein Binding; D011510:Proteolipids / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases
C118416:E8 protein, Bovine papillomavirus; D009856:Oncogene Proteins, Viral; D011510:Proteolipids; C082471:ductin; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases