Structure of the RNA inside the vesicular stomatitis virus nucleocapsid.
The structure of the viral RNA (vRNA) inside intact nucleocapsids of vesicular stomatitis virus was studied by chemical probing experiments. Most of the Watson-Crick positions of the nucleotide bases of vRNA in intact virus and in nucleoprotein (N)-RNA template were accessible to the chemical probes and the phosphates were protected. This suggests that the nucleoprotein binds to the sugar-phosphate backbone of the RNA and leaves the Watson-Crick positions free for the transcription and replication activities of the viral RNA-dependent RNA polymerase. The same architecture has been proposed for the influenza virus nucleocapsids. However, about 5% of the nucleotide bases were found to be relatively nonreactive towards the chemical probes and some bases were hyperreactive. The pattern of reactivities was the same for RNA inside virus and for RNA in N-RNA template that was purified over a CsCl gradient and which had more than 94% of the polymerase and phosphoprotein molecules removed. All reactivities were more or less equal on naked vRNA. This suggests that the variations in reactivity towards the chemical probes are caused by the presence of the nucleoprotein.
Iseni
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European Molecular Biology Laboratory Grenoble Outstation, France.
Baudin
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Blondel
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Ruigrok
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D000447:Aldehydes; D000818:Animals; D001483:Base Sequence; D002074:Butanones; D002999:Clone Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D019251:Nucleocapsid / Q000737:chemistry* / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D013463:Sulfuric Acid Esters; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000737:chemistry* / Q000235:genetics
D000447:Aldehydes; D002074:Butanones; D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral; D013463:Sulfuric Acid Esters; C005135:kethoxal; C007482:dimethyl sulfate