A spectrophotometric method for assay of tannase using rhodanine.
A method for assay of microbial tannase (tannin acyl hydrolase) based on the formation of chromogen between gallic acid and rhodanine is reported. Unlike the previous protocols, this method is sensitive up to gallic acid concentration of 5 nmol and has a precision of 1.7% (relative standard deviation). The assay is complete in a short time, very convenient, and reproducible.
Sharma
S
S
Department of Chemistry and Biochemistry, College of Basic Sciences, H.P.K.V., Palampur, H.P., India.
Bhat
T K
TK
Dawra
R K
RK
D001234:Aspergillus niger / Q000201:enzymology; D001236:Aspergillus oryzae / Q000201:enzymology; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000378:metabolism; D002863:Chromogenic Compounds; D005069:Evaluation Studies as Topic; D005707:Gallic Acid / Q000031:analogs & derivatives; D015203:Reproducibility of Results; D012236:Rhodanine; D013053:Spectrophotometry / Q000379:methods*; D013379:Substrate Specificity
D002863:Chromogenic Compounds; C052082:methyl gallate; D005707:Gallic Acid; D012236:Rhodanine; D002265:Carboxylic Ester Hydrolases; C022711:tannase