title: Circadian modulation of dopamine receptor responsiveness in Drosophila melanogaster.
abstract: We investigated the circadian function of Drosophila dopamine receptors by using a behaviorally active decapitated preparation that allows for direct application of drugs to the nerve cord. Quinpirole, a D2-like dopamine receptor agonist, induces reflexive locomotion in decapitated flies. We show that the amount of locomotion induced changes as a function of the time of day, with the highest responsiveness to quinpirole during the subjective night. Furthermore, dopamine receptor responsiveness is under circadian control and depends on the normal function of the period gene. The head pacemaker is at least partly dispensable for the circadian modulation of quinpirole-induced locomotion, because changes in agonist responsiveness persist in decapitated flies that are aged for 12 h. This finding suggests a role for the period-dependent molecular oscillators in the body in the modulation of amine receptor responsiveness.
authors.affiliation: Department of Biology, University of Virginia, Charlottesville, VA 22903, USA.
authors.forename: R
authors.initials: R
authors.lastname: Andretic
authors.forename: J
authors.initials: J
authors.lastname: Hirsh
mesh_terms: D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D018491:Dopamine Agonists / Q000494:pharmacology; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000378:metabolism*; D008124:Locomotion / Q000187:drug effects; D009687:Nuclear Proteins / Q000235:genetics; D056950:Period Circadian Proteins; D019257:Quinpirole / Q000494:pharmacology; D011954:Receptors, Dopamine / Q000378:metabolism*
chemical_list: D018491:Dopamine Agonists; D029721:Drosophila Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D011954:Receptors, Dopamine; D019257:Quinpirole