Phosphocholine and phosphoethanolamine during chick embryo myogenesis: a (31)P-NMR study.
Elevated contents of phosphoethanolamine (Etn-P) and/or phosphocholine (Cho-P), a common feature of most tumours with respect to normal counterparts, may also occur in non-cancerous proliferating tissues. The significance of these alterations in relation to cell proliferation, differentiation and maturation is scarcely understood. In this work, the Cho-P and Etn-P pools were measured by (31)P-NMR in extracts of chick embryo pectoral muscle at different days of development. The average concentration of these metabolites exhibited the highest values (respectively, 1.5 and 3.0 micromol/mg DNA) on days 9-11 and decreased at later stages of myogenesis. While, however, Cho-P maintained substantial levels (above 1.0 micromol/mg DNA) also during myotube formation (days 11-18) and stepwise decreased (to about 0.5 micromol/mg DNA) upon fibres' maturation, Etn-P gradually decreased between day 11 and hatching time (down to about 0.2 micromol/mg DNA). These results demonstrate that significant changes may occur in the steady-state pools of these metabolites during normal in vivo cellular development and differentiation, and are consistent with: (a) high rates of phospholipid biosynthesis reported in the literature for proliferating myoblasts; (b) sustained phosphatidylcholine synthesis maintained also during myoblast fusion; and (c) decreased requirement of phospholipid synthesis in the last phase of in ovo myofibre maturation.
Granata
F
F
Department of Cell Biology and Development, University 'La Sapienza', 00185, Rome, Italy.
Iorio
E
E
Carpinelli
G
G
Giannini
M
M
Podo
F
F
D000818:Animals; D002642:Chick Embryo; D004247:DNA / Q000032:analysis; D004983:Ethanolamines / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D009132:Muscles / Q000196:embryology* / Q000378:metabolism; D010767:Phosphorylcholine / Q000032:analysis*; D013997:Time Factors; D014020:Tissue Extracts / Q000737:chemistry
D004983:Ethanolamines; D014020:Tissue Extracts; D010767:Phosphorylcholine; C005448:phosphorylethanolamine; D004247:DNA