title: Effects of fenfluramine combined with electroacupuncture on monoamine release in periaqueductal gray of rat brain.
abstract: OBJECTIVE
  To study the changes of monoamines in ventrolatoral periaqueductal gray of rat brain before and after electroacupuncture (EA) analgesia (EAA) was enhanced by fenfluramine (Fen), a 5-hydroxytryptamine (5-HT) releaser.
  
  
  METHODS
  Monoamines were collected by in vivo microdialysis and measured by HPLC connected with electrochemical detector.
  
  
  RESULTS
  The level of norepinephrine (Nor) after EA was decreased (P < 0.05 vs NS group). The contents of 5-HT, 5-hydroxyindol acetic acid (5-HIAA), dopamine (DA), and homovanillic acid (HVA) in periaqueductal gray dialysate were increased (P < 0.05 vs NS group). When Fen was combined with EA, the level of 5-HT and 5-HIAA were further increased (P < 0.05 vs NS + EA group). There was no obvious change of Nor, DA, and HVA.
  
  
  CONCLUSIONS
  Fen potentiating EAA may be related to further activation of serotoninergic system.
authors.affiliation: Department of Neurobiology, Shanghai Medical University, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: C B
authors.initials: CB
authors.lastname: Zhu
authors.forename: H N
authors.initials: HN
authors.lastname: Chen
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhu
authors.forename: G C
authors.initials: GC
authors.lastname: Wu
authors.forename: S F
authors.initials: SF
authors.lastname: Xu
mesh_terms: D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D015671:Electroacupuncture; D005277:Fenfluramine / Q000494:pharmacology*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D010487:Periaqueductal Gray / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism; D018490:Serotonin Agents / Q000494:pharmacology*
chemical_list: D015306:Biogenic Monoamines; D018490:Serotonin Agents; D005277:Fenfluramine; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid