Bromoenol lactone enhances the permeabilization of rat submandibular acinar cells by P2X7 agonists.
The permeabilizing effect of P2X(7) agonists was tested in rat submandibular acinar cells using the uptake of ethidium bromide as an index. The uptake of ethidium bromide by acini incubated at 37 degrees C in the presence of 1 mM ATP increased with time and reached after 5 min about 10% of maximal uptake measured in the presence of digitonin. The response to ATP was dose-dependent (half-maximal concentration around 40 microM) and it was decreased when the temperature was lowered to 25 degrees C. Benzoyl-ATP reproduced the response to ATP (half-maximal concentration around 10 microM). UTP or 2-methylthioATP had no effect. The permeabilization in response to ATP was blocked by oxidized ATP and by magnesium and inhibited by Coomassie blue. ATP increased the activity of a calcium-insensitive phospholipase A(2) (iPLA(2)). Bromoenol lactone (BEL) inhibited the iPLA(2) stimulated by ATP but potentiated the uptake of ethidium bromide in response to the purinergic agonist. From these results it is concluded that the activation of P2X(7) receptors permeabilizes rat submandibular acinar cells. The pore-forming activity of the receptor might be negatively regulated by the concomitant activation of the iPLA(2) by the receptor.
Chaib
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N
Laboratoire de Biochimie et de Biologie Cellulaire, Institut de Pharmacie, Universite libre de Bruxelles, Bruxelles, Belgium.
Kabré
E
E
Alzola
E
E
Pochet
S
S
Dehaye
J P
JP
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D004357:Drug Synergism; D004996:Ethidium / Q000493:pharmacokinetics; D007202:Indicators and Reagents / Q000493:pharmacokinetics; D008297:Male; D009281:Naphthalenes / Q000494:pharmacology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism; D058910:Purinergic P2 Receptor Agonists; D011753:Pyrones / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D058486:Receptors, Purinergic P2X7; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013696:Temperature
D007202:Indicators and Reagents; D009281:Naphthalenes; C546470:P2rx7 protein, rat; D010726:Phosphodiesterase Inhibitors; D058910:Purinergic P2 Receptor Agonists; D011753:Pyrones; D058486:Receptors, Purinergic P2X7; C068314:6-(bromomethylene)tetrahydro-3-(1-naphthaleneyl)-2H-pyran-2-one; D000255:Adenosine Triphosphate; D010741:Phospholipases A; D004996:Ethidium