Partial cDNAs encoding G-protein alpha subunits in the rat vestibular periphery.
To begin understanding what G-proteins are involved in signal transduction in the vestibular periphery, the expression of Galpha subunits in rat primary afferent neurons (Scarpa's ganglia) and end-organs was studied. Reverse transcription-polymerase chain reaction (RT-PCR) with degenerate primers corresponding to two conserved regions of the Galpha protein coding sequence produced partial cDNAs encoding two distinct forms of Galpha(s) subunit (Galpha(s2) and anove) Galpha(s2) subunit,GenBank accession number AF1841510); and two forms of Galpha(i2) subunits. A novel truncated form of Galpha(i2) (designated Galpha(i2(vest)),Gen Bank accession number AF189020) was detected in the vestibular periphery. Galpha(i2(vest)) was also expressed in rat cerebellum and heart. The possible role of the identified Galpha protein cDNAs in the function of the vestibular periphery is discussed.
Wackym
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PA
Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, 9200 W. Wisconsin Avenue, Milwaukee, WI 53226, USA. wackym@mcw.edu
Troyanovskaya
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M
Popper
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P
D000595:Amino Acid Sequence; D000818:Animals; D001306:Auditory Pathways / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics*; D017950:Ganglia, Sensory / Q000378:metabolism*; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014722:Vestibule, Labyrinth / Q000294:innervation*
D017931:DNA Primers; D018076:DNA, Complementary; D020033:Protein Isoforms; D019205:GTP-Binding Protein alpha Subunits, Gs