Characterization of the Tn916-like transposon Tn3872 in a strain of abiotrophia defectiva (Streptococcus defectivus) causing sequential episodes of endocarditis in a child.
Clinical blood isolates from three sequential episodes of endocarditis occurring over a 6-month period in a child with a malformative cardiopathy were investigated. All isolates identified as Abiotrophia defectiva were resistant to erythromycin-clindamycin and to tetracycline-minocycline, due to the presence of sequences homologous to the erythromycin resistance gene ermB and to the tetracycline resistance gene tet(M), respectively. These resistance genes were located on a chromosomally borne composite Tn916-related transposon. These results demonstrate the involvement of conjugative transposons in the dissemination of antibiotic resistance in the genus Abiotrophia.
Poyart
C
C
Laboratoire Mixte Pasteur-Necker de Recherche sur les Streptocoques et Streptococcies, Faculté de Médecine Necker-Enfants Malades, France. epoyart@pasteur.fr
Quesne
G
G
Acar
P
P
Berche
P
P
Trieu-Cuot
P
P
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015139:Blotting, Southern; D002675:Child, Preschool; D002981:Clindamycin / Q000494:pharmacology; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D004917:Erythromycin / Q000494:pharmacology; D006801:Humans; D008297:Male; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008911:Minocycline / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000382:microbiology*; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D013753:Tetracycline Resistance / Q000235:genetics
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D002981:Clindamycin; D004917:Erythromycin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D008911:Minocycline