title: AcrD of Escherichia coli is an aminoglycoside efflux pump.
abstract: AcrD, a transporter belonging to the resistance-nodulation-division family, was shown to participate in the efflux of aminoglycosides. Deletion of the acrD gene decreased the MICs of amikacin, gentamicin, neomycin, kanamycin, and tobramycin by a factor of two to eight, and DeltaacrD cells accumulated higher levels of [(3)H]dihydrostreptomycin and [(3)H]gentamicin than did the parent strain.
authors.affiliation: Departments of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206, USA.
authors.forename: E Y
authors.initials: EY
authors.lastname: Rosenberg
authors.forename: D
authors.initials: D
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Nikaido
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests
chemical_list: C000588785:AcrD protein, E coli; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins