title: Structure-function relationships of temporins, small antimicrobial peptides from amphibian skin.
abstract: Temporins, antimicrobial peptides of 10-13 residues, were isolated from secretions of Rana temporaria [Simmaco, M., Mignogna, G., Canofeni, S., Miele, R., Mangoni, M.L. & Barra, D. (1996) Eur. J. Biochem. 242, 788-792]. These molecules are specific to this amphibian species, which is also able to secrete on its skin other antimicrobial peptides similar to those found in different Rana species. The effect of temporins A, B and D (13 residues, net charge +2), and H (10 residues, net charge +1 and +2, respectively) against both artificial membranes of differing lipid composition and bacteria has been investigated in order to gain insight into their mechanisms of action. The results indicate that: the lytic activity of temporins is not greatly affected by the membrane composition; temporins A and B allow the leakage of large-size molecules from the bacterial cells; temporin H renders both the outer and inner membrane of bacteria permeable to hydrophobic substances of low molecular mass; and temporin D, although devoid of antibacterial activity, has a cytotoxic effect on erythrocytes. The results allow important conclusions to be drawn about the minimal structural requirements for lytic efficiency and specificity of temporins.
authors.affiliation: Dipartimento di Scienze Biomediche, Università 'G. D'Annunzio', Chieti, Italy.
authors.forename: M L
authors.initials: ML
authors.lastname: Mangoni
authors.forename: A C
authors.initials: AC
authors.lastname: Rinaldi
authors.forename: A
authors.initials: A
authors.lastname: Di Giulio
authors.forename: G
authors.initials: G
authors.lastname: Mignogna
authors.forename: A
authors.initials: A
authors.lastname: Bozzi
authors.forename: D
authors.initials: D
authors.lastname: Barra
authors.forename: M
authors.initials: M
authors.lastname: Simmaco
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002942:Circular Dichroism; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010455:Peptides; D010743:Phospholipids / Q000737:chemistry; D011896:Rana temporaria; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D010743:Phospholipids