title: Role of Haemophilus influenzae in intra-amniotic infection in patients with preterm rupture of membranes.
abstract: Haemophilus spp. were isolated from the amniotic fluid of eight of 110 consecutive women with preterm premature rupture of membranes (PROM) between 1992 and 1998. Isolates were nontypeable and classified according to biochemical test results as Haemophilus influenzae biotype I (n = 1), biotype II (n = 4), biotype III (n = 1) or biotype IV (n = 2). Primers recognizing specific sequences in the 16S rRNA of the cryptic genospecies of Haemophilus were employed to amplify the DNA of the eight isolates. One isolate classified as Haemophilus influenzae biotype II was confirmed as belonging to the genital cryptic species. Infectious morbidity occurred in five women and two newborns and was associated in most cases with biotype II.
authors.affiliation: Universidad de Chile, Facultad de Medicina, Instituto de Ciencias Biomédicas, Programa de Microbiología, Santiago.
authors.forename: M A
authors.initials: MA
authors.lastname: Martínez
authors.forename: A
authors.initials: A
authors.lastname: Ovalle
authors.forename: M T
authors.initials: MT
authors.lastname: Ulloa
authors.forename: R M
authors.initials: RM
authors.lastname: Vidal
mesh_terms: D000653:Amniotic Fluid / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000453:epidemiology / Q000382:microbiology*; D006192:Haemophilus Infections / Q000453:epidemiology* / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology; D011256:Pregnancy Outcome; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S