Glucose and lactate concentration determination on single microsamples by Fourier-transform infrared spectroscopy.
This study is the first to assess the analytic potential of Fourier-transform infrared (FT-IR) spectroscopy in determining exercise-induced metabolic changes, such as glucose and lactate serum concentrations, with single 50 microL blood microsamples. One-hundred ninety-eight capillary blood samples were taken at rest (rest serum) and after rowing exercises at different intensities (exercise serum) to obtain a wide range of lactate concentrations. A quantitative method is described with FT-IR spectroscopy involving only dilution and dessiccation of serum samples. Within serum spectra, an absorption band was strongly specific of glucose (1033 cm(-1)) that allowed the determination of its concentration (r = 0.97; P < .001 with reference values). Once we had substrated measured glucose absorption in serum spectra, one other absorption band seemed to be specific for lactate (1127 cm(-1)), which allowed the determination of the concentration of this metabolite (r = 0.96; P < .001 with reference values). The convenience of a capillary blood sampling with the strong accuracy of FT-IR measurements is of particular interest for medicinal and biologic concerns.
Petibois
C
C
INSERM U 443, Equipe de Chimie Bio-Organique, Université Victor Segalen Bordeaux 2, France.
Melin
A M
AM
Perromat
A
A
Cazorla
G
G
Déléris
G
G
D001786:Blood Glucose / Q000032:analysis*; D001800:Blood Specimen Collection / Q000379:methods; D002196:Capillaries; D015444:Exercise / Q000502:physiology*; D006801:Humans; D007773:Lactates / Q000097:blood*; D010807:Physical Endurance / Q000502:physiology*; D012044:Regression Analysis; D012146:Rest / Q000502:physiology; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D001786:Blood Glucose; D007773:Lactates