Surface-modification of polystyrene-microtitre plates via grafting of glycidylmethacrylate and coating of poly-glycidylmethacrylate.
Photografting of glycidylmethacrylate (GMA) onto commercially available polystyrene-microtitre plates was carried out in methanol as well as butanol with benzophenone (BP) as photoinitiator. Alternatively, prepolymers of polyglycidylmethacrylate (PGMA) were synthesized in methanol with azo-iso-butyrodinitrile (AIBN) as initiator and dip-coated onto polystyrene-microtitre plates. Both modification methods were tested in order to reach a high binding capacity for proteins. Peroxidase was used as model protein. In addition, the immobilization of myelin basic protein (MBP) to epoxy-modified microtitre plates is shown and a MBP-based ELISA has been developed.
Eckert
A W
AW
Clinic of Craniomaxillofacial Surgery, Oral and Maxillofacial Surgery, Martin Luther University Halle Wittenberg, Germany.
Gröbe
D
D
Rothe
U
U
D001672:Biocompatible Materials; D004797:Enzyme-Linked Immunosorbent Assay; D004852:Epoxy Compounds / Q000737:chemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008689:Methacrylates / Q000737:chemistry; D004676:Myelin Basic Protein / Q000032:analysis / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000737:chemistry; D011137:Polystyrenes; D012997:Solvents; D013499:Surface Properties
D001672:Biocompatible Materials; D004852:Epoxy Compounds; D007202:Indicators and Reagents; D008689:Methacrylates; D004676:Myelin Basic Protein; D011109:Polymethacrylic Acids; D011137:Polystyrenes; D012997:Solvents; C042535:polyglycidyl methacrylate; C007870:glycidyl methacrylate