Sea snake Hydrophis cyanocinctus venom. II. Histopathological changes, induced by a myotoxic phospholipase A2 (PLA2-H1).
A toxic phospholipase A2 (PLA2-H1), isolated from the venom of the sea snake Hydrophis cyanocinctus, was tested for its ability to induce myonecrosis and histopathological changes in albino rats and mice. Induction of myonecrosis was demonstrated by their ability to release creatine kinase (CK) from damaged muscle fibers and direct histopathological examination of the injected muscles (i.m.). PLA2-H1 exhibits intense myonecrosis characterized by the changes including, necrosis and edematous appearance with cellular infiltrate, vacuolation and degenerated muscle cells with delta lesions and heavy edema in between the cells. No myoglobinuria was noted in any group of animals. The purified PLA2-H1 was also administered intraperitoneally into the experimental animals and tissue samples were taken at several time intervals. Light microscopic examination of the kidney sections revealed severe damage, evident by focal tubular necrosis, complete disquamation of epithelial lining and epithelial degeneration of tubules in all test animals. Light micrographs of liver sections after 24 h of injection shows fatty infiltration in parenchyma and squashed hepatocytes, while after 48 h, fatty vacuolation of parenchyma in a generalized pattern was observed. Furthermore, sections of the lungs of the same group of animals (48 h) show dilated bronchia and marked infiltration of inflammatory cells within alveoli. Our results suggest that the purified PLA2-H1 induced moderate myotoxicity in muscles and mild histopathological changes in other vital organs without myoglobinuria.
Ali
S A
SA
International Centre for Chemical Sciences, HEJ Research Institute of Chemistry, University of Karachi, Pakistan. saali66@hotmail.com
Alam
J M
JM
Abbasi
A
A
Zaidi
Z H
ZH
Stoeva
S
S
Voelter
W
W
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000473:pathology; D003402:Creatine Kinase / Q000378:metabolism; D004546:Elapid Venoms / Q000737:chemistry / Q000201:enzymology*; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D009336:Necrosis; D009393:Nephritis / Q000139:chemically induced / Q000473:pathology; D010741:Phospholipases A / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D054467:Phospholipases A2; D011014:Pneumonia / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D018675:Toxicity Tests
D004546:Elapid Venoms; D003402:Creatine Kinase; D010741:Phospholipases A; D054467:Phospholipases A2