[Analysis on speciems of serum and urine in 66 cases of early leptospirosis by PCR and biotin-AMPPD hybridization].
We used polymerase chain reaction (PCR) and Biotin-AMPPD hybridization to detect leptospiral DNA in blood and urine samples in 66 patients at the early stage of leptospirosis. The results showed that PCR and Biotin-AMPPD hybridization not only ruled out the non-specific PCR amplification and increased the reliability for clinical specineus of leptospirosis but also raised the detecting sensibility (from 71.3% to 86.1%). The positive rates of PCR resulted from serum and urine showed no statistical difference; therefore urine sample is worthy of application and dissemination for detecting leptospires at the early stage of leptospirosis. Urine sample is easier for one to collect, preserve and has less intervention. The primers G1, G2 are optimal opplication of PCR in epdemic areas in China.
Xiong
Q
Q
Research Unit of Leptospirosis, School of Basic Medical Sciences, Chengdu.
Bao
L
L
Zhang
M
M
Li
S
S
Wu
W
W
Wan
B
B
Terpstra
W J
WJ
Hartskeerl
R
R
D001710:Biotin; D004269:DNA, Bacterial / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007919:Leptospira / Q000235:genetics; D007922:Leptospirosis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D013997:Time Factors
D004269:DNA, Bacterial; D001710:Biotin