title: Relationship between total and free cellular Mg(2+) during metabolic stimulation of rat cardiac myocytes and perfused hearts.
abstract: The changes in total Mg were compared with changes in cytosolic free Mg(2+) during metabolic stimulation of collagenase-dispersed rat cardiac myocytes or Langendorff-perfused rat hearts. In myocytes the addition of agents leading to cAMP increase or protein kinase C activation results in a loss or gain of more than 5% of total Mg content within 3 min (i.e., 3-4 nmol Mg/mg protein). Under the same conditions, changes in cytosolic free Mg(2+) measured with fluorescent indicator are small and result in changes of cytosolic free Mg(2+) equivalent to 90-140 microM. In perfused hearts, beta-adrenergic stimulation results in a loss of total Mg larger than 0.5 micromol per gram of heart corresponding to 9% loss of total Mg content of the heart (estimated to be 5.8 micromol). Under these conditions there is no change in cytosolic free Mg(2+) or the major buffer of cytosolic Mg(2+), ATP, as measured by (31)P NMR. These data suggest that a major redistribution of total Mg occurs in intracellular organelles or in cytosolic buffers in order to maintain cytosolic free Mg(2+) relatively unchanged during the observed cellular massive translocation of total Mg. Hence, Mg(2+) may regulate metabolic functions not within the cytosol but in locations where its concentration oscillates, such as extracellular fluid and intracellular compartments.
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106-4970, USA.
authors.forename: M
authors.initials: M
authors.lastname: Fatholahi
authors.forename: K
authors.initials: K
authors.lastname: LaNoue
authors.forename: A
authors.initials: A
authors.lastname: Romani
authors.forename: A
authors.initials: A
authors.lastname: Scarpa
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004075:Diglycerides / Q000494:pharmacology; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004075:Diglycerides; C075809:1-stearoyl-2-arachidonoylglycerol; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D008274:Magnesium; D002118:Calcium; D009638:Norepinephrine