Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatis.
Using polystyrene microspheres coated with heparin or heparan sulfate, it was shown that coated microspheres specifically bound eukaryotic cells and were endocytosed by nonprofessional phagocytic cells. Coated microspheres displayed properties of binding to eukaryotic cells that were similar to those of chlamydiae, and the microspheres were competitively inhibited by chlamydial organisms. Endocytosis of heparin-coated beads resulted in the tyrosine phosphorylation of a similar set of host proteins as did endocytosis of chlamydiae; however, unlike viable chlamydial organisms, which prevent phagolysosomal fusion, endocytosed beads were trafficked to a lysosomal compartment. These findings suggest that heparin-coated beads and Chlamydia trachomatis enter eukaryotic cells by similar pathways.
Stephens
R S
RS
Program in Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA. rss@uclink4.berkeley.edu
Fawaz
F S
FS
Kennedy
K A
KA
Koshiyama
K
K
Nichols
B
B
van Ooij
C
C
Engel
J N
JN
D001422:Bacterial Adhesion; D002692:Chlamydia trachomatis / Q000502:physiology*; D006367:HeLa Cells; D006493:Heparin / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008863:Microspheres; D010766:Phosphorylation; D014443:Tyrosine / Q000378:metabolism
D006493:Heparin; D006497:Heparan Sulfate; D014443:Tyrosine