Use of PCR/DNA probes to identify circumsporozoite genotype of Plasmodium vivax in China.
The paper reports the result of identifying cirumsporozoite (CS) genotype of Plasmodium vivax by using PCR/DNA probe labeled with biotin. The sensitivity of this method to detect patient blood samples was 0.2 parasite/microl and also with high specific to P. vivax. CS genes from 52 blood samples collected from patients with P. vivax in Hainan and Yunnan Provinces were amplified by PCR and 49 were positive by gel-e electrophoresis analysis, positive rate was 94%. Then the amplified CS genes further were probed with special oligoprobes (PV210 and PV247) that hybridized with the predominant CS repeat region and the variant CS repeat region. The results showed 46 (88.5%) PV210 positive and 6 (11.5%) PV247 positive; 2 hybridized with both probes. The variant genotype was present only in samples from Yunnan Province. The above results showed that the PCR/DNA probe labeled with biotin was highly sensitive and specific to P. vivax and found a CS variant genotype of P. vivax in Yunnan Province of China.
Han
G D
GD
Shandong Institute of Parasitic Diseases, Jining, China.
Zhang
X J
XJ
Zhang
H H
HH
Chen
X X
XX
Huang
B C
BC
D000818:Animals; D016022:Case-Control Studies; D002681:China; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood / Q000469:parasitology*; D010966:Plasmodium vivax / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015342:DNA Probes; D016054:DNA, Protozoan