Cloning and characterization of the unusual cyclin gene from an amphidiploid of Nicotiana glauca-Nicotiana langsdorffii hybrid.
Cyclins play an important role in the regulation of cell cycle progression in eukaryotic cells. As an aid to understanding the molecular nature of unregulated cell proliferation, a cDNA clone encoding a cyclin gene, GTcyc, was identified from genetic tumors. The clone contained 1095 bp including a 24 base poly(A) tail. GTcyc is an unusual cyclin gene, distantly related to mammalian cyclin D genes having 21-25% identity within the cyclin box. Northern blots showed that the genetic tumors express high levels of GTcyc relative to non-tumor hybrid tissues. Southern analysis suggests that GTcyc may be contained one or two families in genetic tumors.
Wang
M H
MH
Department of Pharmacy, College of Pharmacy, Seoul National University, South Korea. mhwang@plaza1.snu.ac.kr
Doonan
J H
JH
Sastry
G R
GR
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016213:Cyclins / Q000235:genetics*; D018076:DNA, Complementary; D004171:Diploidy; D006824:Hybridization, Genetic; D008969:Molecular Sequence Data; D010940:Plant Proteins; D010947:Plants, Toxic; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*
D016213:Cyclins; D018076:DNA, Complementary; C404506:GTcyc protein, Nicotiana; D010940:Plant Proteins