title: yam8(+), a Schizosaccharomyces pombe gene, is a potential homologue of the Saccharomyces cerevisiae MID1 gene encoding a stretch-activated Ca(2+)-permeable channel.
abstract: The Saccharomyces cerevisiae MID1 gene encodes a stretch-activated Ca(2+)-permeable channel. In a protein database, we found a Schizosaccharomyces pombe gene whose predicted protein shows 26% identical and 62% similar to the Mid1 channel in amino acid sequence. cDNA derived from this gene, designated yam8(+), was isolated by reverse transcription-polymerase chain reaction (RT-PCR). Further analysis showed that the Yam8 protein consists of 486 amino acids and has 6 hydrophobic segments. The yam8(+) cDNA, placed under the S. cerevisiae TDH3 promoter, partially complemented the mating pheromone-induced death (mid) phenotype of the S. cerevisiae mid1 mutant. The expression of the yam8(+) cDNA in the mid1 mutant cells partially remediated the mid phenotype and resulted in a slight increase in Ca(2+) uptake activity. These findings suggest that Yam8 is a potential homologue of Mid1.
authors.affiliation: Research Institute for Biological Sciences, 7549-1 Yoshikawa, Kayo, Joubo, Okayama, 716-1241, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tasaka
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: C
authors.initials: C
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Mino
authors.forename: N
authors.initials: N
authors.lastname: Uozumi
authors.forename: N
authors.initials: N
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Muto
authors.forename: H
authors.initials: H
authors.lastname: Iida
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D015220:Calcium Channels; D017931:DNA Primers; D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; C090463:MID1 protein, S cerevisiae; D008562:Membrane Glycoproteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C405644:YAM8 protein, S pombe