[Determination of glycosylated hemoglobin in human erythrocytes by fast protein liquid chromatography system].
The chromatography method for analysis of glycosylated hemoglobin(HbA1c) in human erythocytes was established by using a fast protein liquid chromatography system(FPLC) and a Mono S column. The average intrabatch and interbatch coefficients of variations (n = 3) were 2.5% and 4.8%, respectively. There was a positive correlation between the results of FPLC and routine microcolumn method (r = 0.413, P < 0.02). Using FPLC method, the HbA1c levels in festing blood of 54 normal donors and 26 diabetics on admission were 4.6 +/- 1.19(%) and 10.2 +/- 3.45(%), respectively. A significant difference was found statistically between the results of two groups (t = 10.8, P < 0.01).
Wen
J
J
Institute of Metabolism and Endocrinology, Second Affiliated Hospital, Hunan Medical University, Changsha.
Zhou
J
J
Wu
H
H
Zhou
Z
Z
Dai
S
S
Zi
J
J
D000293:Adolescent; D000328:Adult; D002845:Chromatography / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood*; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged
D006442:Glycated Hemoglobin