Alterations of phosphatidylinositol signal transduction pathway in hepatic mitochondria following aflatoxin B1 administration.
A single dose of aflatoxin B1 (7 mg/kg body wt) to male rats significantly stimulated the turnover of mitochondrial phosphoinositides 1-7 hr following its administration. The elevation of phosphatidylinositol 3,4,5-trisphosphate was most pronounced whose level continued to be moderately high even at 17 hr period. The level of diacylglycerol showed a marked increase from 4 hr till 7 hr after carcinogen treatment, whereas that of inositol 1,4,5-trisphosphate recorded an increase with a maximum at 7 hr followed by a gradual decrease to near normal level at 24 hr period. The activation of phosphatidylinositol cycle together with an activation of PI 3-kinase, whose product PIP3 is known to be involved in apoptosis might contribute to the early step in the manifestation of toxicity and/or carcinogenicity.
Pasupathy
K
K
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India. rbbd@magnum.barc.ernet.in
Krishna
M
M
Bhattacharya
R K
RK
D016604:Aflatoxin B1 / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
D010716:Phosphatidylinositols; D016604:Aflatoxin B1