Cloning and expression of cytochrome P450 genes belonging to the CYP4 family and to a novel family, CYP48, in two hymenopteran insects, Trichogramma cacoeciae and Apis mellifera.
Cytochrome P450 partial sequences were isolated by PCR using genomic DNA from two hymenopteran insects of agronomical importance, Trichogramma cacoeciae, a parasitoid wasp, and Apis mellifera, the honeybee. Four new P450 genes were identified: one honeybee gene belongs to the CYP4 family and was named CYP4G11; the three other genes were from Trichogramma and belong to the CYP4 family (CYP4G12) and to a novel family, the CYP48 one (CYP48A1 and CYP48A2). The four genes contain a short intron (72-95 bp) at the same position as already described for other insect species. The two genes CYP48A1 and CYP48A2 have a supernumary intron (57-71 bp) upstream the first one. Only the two CYP4 genes were constitutively transcribed, at a high level for CYP4G12 and at a low level for CYP4G11. No expression was observed for CYP48A1 and CYP48A2.
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INRA-IFR 38 "Réponse des organismes aux stress physico-chimiques et biologiques,", Unité Santé Végétale et Environnement, 123 boulevard Francis Meilland, Antibes Cedex, 06606, France. tares@antibes.inra.fr
Bergé
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Amichot
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D017344:Genes, Insect; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014863:Wasps / Q000201:enzymology* / Q000235:genetics*
D017931:DNA Primers; D003577:Cytochrome P-450 Enzyme System