A DPDPE-induced enhancement of inward rectifier potassium current via opioid receptor in neuroblastomaxglioma NG108-15 cells.
The effect of a delta-selective opioid agonist, DPDPE([D-Pen(2, 5)]-enkephalin), on the inward rectifier potassium current (I(KIR)) of NG108-15 cell was studied by whole cell voltage-clamp technique. It was found that microM DPDPE increased the amplitude and delayed the activation and inactivation of I(KIR) rapidly and reversibly. These effects could be reversed by naloxone, but were still obtained in pertussis toxin (PTX) preincubated cells or when using GDP-betas (guanosine 5'-o-[2-thio] diphoaphate) containing electrodes to block the G-protein coupled events. The above results suggest that DPDPE-induced change of I(KIR) is mediated by delta-opioid receptor but does not involve G-proteins.
Shi
Y
Y
Key Laboratory of Neurobiology, Shanghai Institute of Physiology, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, PR China. ylshi@server.shcnc.ac.cn
Huang
F S
FS
Chen
W Y
WY
Wu
Y
Y
Tang
Y
Y
Hu
Q
Q
D000818:Animals; D004553:Electric Conductivity; D004566:Electrodes; D020881:Enkephalin, D-Penicillamine (2,5)- / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D018408:Patch-Clamp Techniques / Q000295:instrumentation; D037342:Pertussis Toxin; D015221:Potassium Channels / Q000502:physiology*; D024661:Potassium Channels, Inwardly Rectifying; D051381:Rats; D011957:Receptors, Opioid / Q000502:physiology*; D013873:Thionucleotides; D013997:Time Factors; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D009292:Narcotic Antagonists; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D011957:Receptors, Opioid; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; D006153:Guanosine Diphosphate; D009270:Naloxone; C023427:guanosine 5'-O-(2-thiodiphosphate); D020881:Enkephalin, D-Penicillamine (2,5)-; D037342:Pertussis Toxin