Dauricine inhibits redistribution of platelet membrane glycoprotein IV and release of intracellular alpha-granule thrombospondin induced by thrombin.
OBJECTIVE
To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein IV (GPIV) and release of intracellular alpha-granule thrombospondin (TSP) on platelet activation.


METHODS
Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin.


RESULTS
Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPIV and TSP (r = 0.511, P < 0.01). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent.


CONCLUSIONS
Dau inhibited redistribution of GPIV and release of intracellular alpha-granule thrombospondin induced by thrombin.
Guo
T
T
Institute of Hematology, Tongji Medical University, Wuhan. Fly32@hotmail.com
Zhang
Y Z
YZ
Liu
D X
DX
Zou
P
P
Shen
D
D
D000328:Adult; D000470:Alkaloids; D044182:Benzylisoquinolines; D001792:Blood Platelets / Q000378:metabolism; D018955:CD36 Antigens / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D044005:Tetrahydroisoquinolines; D013917:Thrombin / Q000494:pharmacology; D019699:Thrombospondins / Q000378:metabolism*
D000470:Alkaloids; D044182:Benzylisoquinolines; D018955:CD36 Antigens; D007546:Isoquinolines; D010975:Platelet Aggregation Inhibitors; D044005:Tetrahydroisoquinolines; D019699:Thrombospondins; C035934:dauricine; D013917:Thrombin