title: Measurement of interleukin 10 in bronchoalveolar lavage from preterm ventilated infants.
abstract: BACKGROUND
  Interleukin 10 (IL-10) is a cytokine that downregulates inflammation, in part by reducing the production of the proinflammatory cytokines IL-1beta and IL-8. It has been suggested that an inability to produce IL-10 might predispose preterm infants to develop chronic lung disease.
  
  
  OBJECTIVE
  To measure IL-10, IL-1beta, and IL-8 in bronchoalveolar lavage fluid from ventilated preterm infants in a prospective cohort study.
  
  
  METHODS
  17 consecutive newborn infants < or = 29 weeks' gestational age (median, 25; 9 boys) who were ventilated from birth underwent daily bronchoalveolar lavage sampling.
  
  
  RESULTS
  102 samples were collected, of which 57 contained IL-10 in amounts that were comparable with those found previously in ventilated term infants with respiratory failure. Chronic lung disease developed in 9 of the 11 survivors and all 9 infants had produced IL-10. IL-1beta and IL-8 were detected in nearly all samples and were raised throughout the course of sample collection.
  
  
  CONCLUSIONS
  IL-10 is readily detectable in early bronchoalveolar lavage samples from ventilated preterm infants, although it remains unclear whether this cytokine has any influence on the development of chronic lung disease.
authors.affiliation: Child Life and Health, Reproductive Medicine and Developmental Sciences, University of Edinburgh, 20 Sylvan Place, Edinburgh EH9 1UW, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: McColm
authors.forename: B J
authors.initials: BJ
authors.lastname: Stenson
authors.forename: N
authors.initials: N
authors.lastname: Biermasz
authors.forename: N
authors.initials: N
authors.lastname: McIntosh
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D005260:Female; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D016753:Interleukin-10 / Q000032:analysis*; D008173:Lung Diseases, Obstructive / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D008297:Male; D011446:Prospective Studies; D012123:Pulmonary Ventilation; D012121:Respiration, Artificial
chemical_list: D015415:Biomarkers; D016753:Interleukin-10