Determination of phenobarbital in plasma by micellar liquid chromatography.
A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C(18) analytical column, a guard column of similar characteristics, and a 0.03 M CTAB-3% 1-propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 microg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7. 5%.
Martín-Biosca
Y
Y
Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, C/ Vicente A. Estellés s/n, E-46100 Burjassot, València, Spain.
Sagrado
S
S
Villanueva-Camãnas
R M
RM
Medina-Hernández
M J
MJ
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008823:Micelles; D010634:Phenobarbital / Q000097:blood*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D008823:Micelles; D010634:Phenobarbital