title: Quantitative PCR analysis of AMPA receptor composition in two paradigms of global ischemia.
abstract: Quantitative PCR was used to analyse the expression of GluR1, GluR2, GluR2 flip, GluR2 flop and GluR3 mRNA in animals after ischemia and tolerance-inducing ischemia. The ischemic animals showed a decrease in the GluRs to approximately 30%, except for GluR2-flip, which decreased to 75%. The tolerance animals displayed regulation of GluR1 to 75%, GluR2 and GluR2-flop to 283% and 265% respectively. We did not find a correlation between GluR2 regulation and cell loss in the ischemic group. The selective upregulation of GluR2/GluR2 flop in tolerant animals indicates a possible mechanism for enhanced AMPA receptor desensitisation leading to tolerance to ischemia.
authors.affiliation: Laboratory of Neuropathology, Institute of Molecular Pathology, University of Copenhagen, Denmark.
authors.forename: C W
authors.initials: CW
authors.lastname: Alsbo
authors.forename: M L
authors.initials: ML
authors.lastname: Wrang
authors.forename: F F
authors.initials: FF
authors.lastname: Johansen
authors.forename: N H
authors.initials: NH
authors.lastname: Diemer
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D004195:Disease Models, Animal; D015536:Down-Regulation; D005786:Gene Expression Regulation; D008297:Male; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D018091:Receptors, AMPA; C104723:glutamate receptor ionotropic, AMPA 3; C104722:glutamate receptor ionotropic, AMPA 2