Growth of the yeast Saccharomyces cerevisiae on a non-fermentable substrate: control of energetic yield by the amount of mitochondria.
The purpose of this study was to investigate the long-term control of ATP synthesis during the course of Saccharomyces cerevisiae batch grown on lactate, a purely respiratory substrate. For this, we used a respirometric and on-line calorimetric approach to analyse the energetic balances and the control of energetic metabolism during growth. Enthalpic growth yields assessed by enthalpy balance (taking account of substrate consumption, by-product accumulation, biomass formation and heat dissipation) remained constant during the entire exponential growth. Moreover, at the same time, a parallel decrease in basal respiratory rate and enthalpy flux occurred. It is shown that the decrease in respiration corresponds to a decrease in the amount of mitochondria per cell but not to a change of steady state of oxidative phosphorylation. Taking into account the part of energy used for maintenance, it can be concluded that mitochondria by themselves are the major heat dissipative system in a fully aerobic metabolism, and that the decrease in the amount of mitochondria when growth rate decreases leads to an enthalpic growth yield constant.
Dejean
L
L
Institut de Biochimie et Génétique Cellulaires du CNRS, Université Victor Segalen, Bordeaux II, 1 rue Camille Saint Saëns, 33077, Bordeaux, France.
Beauvoit
B
B
Guérin
B
B
Rigoulet
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D002151:Calorimetry; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003470:Culture Media; D004734:Energy Metabolism; D007773:Lactates; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D010101:Oxygen Consumption; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D014475:Uncoupling Agents
D003470:Culture Media; D007773:Lactates; D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate