The initiator element of the Drosophila beta2 tubulin gene core promoter contributes to gene expression in vivo but is not required for male germ-cell specific expression.
The tissue-specific expression of the Drosophila beta 2 tubulin gene ( B2t ) is accomplished by the action of a 14-bp activator element (beta2UE1) in combination with certain regulatory elements of the TATA-less, Inr-containing B2t core promoter. We performed an in vivo analysis of the Inr element function in the B2t core promoter using a transgenic approach. Our experiments demonstrate that the Inr element acts as a functional cis -regulatory element in vivo and quantitatively regulates tissue-specific reporter expression in transgenic animals. However, our mutational analysis of the Inr element demonstrates no essential role of the Inr in mediating tissue specificity of the B2t promoter. In addition, a downstream element seems to affect promoter activity in combination with the Inr. In summary, our data show for the first time the functionality of the Inr element in an in vivo background situation in Drosophila.
Santel
A
A
Zoologie-Entwicklungsbiologie am Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch-Strasse, D-35032 Marburg, Germany. santel@cmgm.stanford.edu
Kaufmann
J
J
Hyland
R
R
Renkawitz-Pohl
R
R
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D005786:Gene Expression Regulation / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020218:Response Elements / Q000235:genetics*; D017384:Sequence Deletion / Q000235:genetics; D013094:Spermatozoa / Q000378:metabolism*; D016385:TATA Box / Q000235:genetics; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics; D014404:Tubulin / Q000235:genetics*
D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D019476:Insect Proteins; D014404:Tubulin; D004247:DNA