Murine Myak, a member of a family of yeast YAK1-related genes, is highly expressed in hormonally modulated epithelia in the reproductive system and in the embryonic central nervous system.
We have cloned a mouse homologue (designated Myak) of the yeast protein kinase YAK1. The 1210 aa open reading frame contains a putative protein kinase domain, nuclear localization sequences and PEST sequences. Myak appears to be a member of a growing family of YAK1-related genes that include Drosophila and human Minibrain as well as a recently identified rat gene ANPK that encode a steroid hormone receptor interacting protein. RNA blot analysis revealed that Myak is expressed at low levels ubiquitously but at high levels in reproductive tissues, including testis, epididymis, ovary, uterus, and mammary gland, as well as in brain and kidney. In situ hybridization analysis on selected tissues revealed that Myak is particularly abundant in the hormonally modulated epithelia of the epididymis, mammary gland, and uterus, in round spermatids in the testis, and in the corpora lutea in the ovary. Myak is also highly expressed in the aqueduct of the adult brain and in the brain and spinal cord of day 12.5 embryos.
Shang
E
E
The Center for Reproductive Sciences, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Wang
X
X
Huang
J
J
Yoshida
W
W
Kuroiwa
A
A
Wolgemuth
D J
DJ
D000818:Animals; D015152:Blotting, Northern; D002490:Central Nervous System / Q000196:embryology / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D005260:Female; D005835:Genitalia / Q000201:enzymology*; D006728:Hormones / Q000378:metabolism; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000201:enzymology; D008297:Male; D008321:Mammary Glands, Animal / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000201:enzymology; D010802:Phylogeny; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology; D014599:Uterus / Q000201:enzymology
D006728:Hormones; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D017346:Protein Serine-Threonine Kinases; C062391:YAK1 protein, S cerevisiae