Detection of single-stranded cohesive ends in the genome of Bacillus thuringiensis temperate phage KK-88.
Cohesive ends (cos sites) were detected in the genome of temperate KK-88 phage in Bacillus thuringiensis after analysis of the phage DNA generated by the 3'-5' exonuclease activity of Klenow fragment of DNA polymerase I. In addition, unlike the 5'-protruding ends of coliphage lambda genome, the ends of KK-88 phage genome were found to be 3'-protruding. The restriction map of the phage genome was also constructed on the basis of position of the cos fragments.
Kanda
K
K
Department of Applied Biological Sciences, Saga University, Japan.
Tachi
M
M
Nishimura
Y
Y
Kato
F
F
Murata
A
A
D001413:Bacillus thuringiensis / Q000821:virology*; D001435:Bacteriophages / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry*; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D016679:Genome, Viral; D015183:Restriction Mapping
D004277:DNA, Single-Stranded; D004279:DNA, Viral