title: On-line size-exclusion chromatography/electrospray ionisation mass spectrometry of aquatic humic and fulvic acids
abstract: Isolated aquatic humic and fulvic acids were analysed with on-line size exclusion chromatography/electrospray ionisation mass spectrometry (SEC/ESI-MS). An eluent composition which enabled electrospray ionisation was identified. The SEC separation improved interpretability of mass spectra and may open up new possibilities for molecular weight determination of humic substances. A linear dose-response relationship over a factor of 20 was obtained and the limit of detection was 50ng/uL for humic and fulvic acids. Spectral changes due to different ionisation conditions (pH and cone voltage) were investigated. A natural water sample from a Swedish lake was analysed. Copyright 2000 John Wiley & Sons, Ltd.
authors.affiliation: Laboratory for Analytical Environmental Chemistry, Institute of Applied Environmental Research, Stockholm University, SE-106 91 Stockholm, Sweden.
authors.initials: L
authors.lastname: Persson
authors.initials: T
authors.lastname: Alsberg
authors.initials: G
authors.lastname: Kiss
authors.initials: G
authors.lastname: Odham