[Analysis of prevalence of point mutations in codon 12 of oncogene K-ras from non-cancerous samples of cervical cytology positive for type 16 or 18 PVH].
Ninety-one non cancerous samples from genital specimens positives for VPH 16 or 18 and 27 non-infected samples as controls were studied. Mutations at codon 12 in K-ras gene was analyzed using enriched alelic PCR technique. Among the samples studied 17.58% showed mutations in this codon. Significant differences were observed between the control group (negative DNA-HPV) and positives DNA-HPV samples (p < 0.01). No differences were found between both viral types in relation to the mutation frequency. The presence of mutations in the K-ras gene in non cancerous cytological samples point out new questions about the role of mutations in proto-oncogenes and the development of cervical cancer.
Golijow
C D
CD
Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina. cgolijow@fcv.medvet.unlp.edu.ar
Mourón
S A
SA
Gómez
M A
MA
Dulout
F N
FN
D002584:Cervix Uteri / Q000821:virology*; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000235:genetics* / Q000821:virology*; D017354:Point Mutation; D015995:Prevalence; D014412:Tumor Virus Infections / Q000235:genetics* / Q000821:virology*; D014626:Vaginal Smears