[The expression of tumor suppressor gene p53 and Rb gene in nasopharyngeal carcinoma].
In order to observe the relation of tumor supperssor gene p53 and Rb gene to nasopharyngeal carcinoma, we investigated p53 gene mutations in exon 7-8 in 33 cases of nasopharygeal carcinoma (NPC), using single-strand conformation ploymorphism analysis of PCR (PCR-SSCP). The data showed p53 mutations in 7 of 33 specimens at exon 8 (21.2%). No mutation in exon 7 was detected. Analysis of Rb gene in NPC tissues by southern blot revealed the deletion and loss of activity of Rb gene in 11 of 13 cases (86.6%). Our results suggest that Rb gene and p53 gene are closely associated with the tumorigenesis of NPC.
Song
Y
Y
Institute of Cancer Research, Chengdu.
Wang
L
L
Huang
G
G
Huang
R
R
Yuan
S
S
Liu
Y
Y
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005091:Exons; D017353:Gene Deletion; D016161:Genes, Retinoblastoma; D016158:Genes, p53; D006801:Humans; D009154:Mutation; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational