title: The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin.
abstract: We have designed a gene cassette for expression of the bleomycin-resistance protein from Streptoalloteichus hindustanus (ShBle) in the extremely halophilic archaeon Haloferax volcanii, and shown that transformed haloarchaea are resistant to bleomycin. Recombinant ShBle was purified by a one-step affinity-chromatography procedure as a correctly folded, dimeric protein. ShBle thus provides a useful haloarchaeal selectable marker and represents the first non-halophilic and soluble heterologous protein to be expressed in the Haloarchaea.
authors.affiliation: CSIRO Health Sciences and Nutrition, and CRC for Diagnostic Technologies, 343 Royal Parade, Parkville, Victoria 3052, Australia. Stewart.Nuttall@hsn.csiro.au
authors.forename: S D
authors.initials: SD
authors.lastname: Nuttall
authors.forename: S E
authors.initials: SE
authors.lastname: Deutschel
authors.forename: R A
authors.initials: RA
authors.lastname: Irving
authors.forename: J A
authors.initials: JA
authors.lastname: Serrano-Gomicia
authors.forename: M L
authors.initials: ML
authors.lastname: Dyall-Smith
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001105:Archaea / Q000187:drug effects / Q000235:genetics*; D019843:Archaeal Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001761:Bleomycin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D019766:Genes, Archaeal; D019621:Haloferax / Q000187:drug effects / Q000235:genetics
chemical_list: D000903:Antibiotics, Antineoplastic; D019843:Archaeal Proteins; D001426:Bacterial Proteins; C099353:phleomycin resistance protein, Streptoalloteichus hindustanus; D001761:Bleomycin