title: Hypoxia, hyperoxia, ischemia, and brain necrosis.
abstract: BACKGROUND
  Human brains show widespread necrosis when death occurs after coma due to cardiac arrest, but not after hypoxic coma. It is unclear whether hypoxia alone can cause brain damage without ischemia. The relationship of blood oxygenation and vascular occlusion to brain necrosis is also incompletely defined.
  
  
  METHODS
  We used physiologically monitored Wistar rats to explore the relationship among arterial blood oxygen levels, ischemia, and brain necrosis. Hypoxia alone (PaO2 = 25 mm Hg), even at a blood pressure (BP) of 30 mm Hg for 15 minutes, yielded no necrotic neurons. Ischemia alone (unilateral carotid ligation) caused necrosis in 4 of 12 rats, despite a PaO2 > 100 mm Hg. To reveal interactive effects of hypoxia and ischemia, groups were studied with finely graded levels of hypoxia at a fixed BP, and with controlled variation in BP at fixed PaO2. In separate series, focal ischemic stroke was mimicked with transient middle cerebral artery (MCA) occlusion, and the effect of low, normal, and high PaO2 was studied.
  
  
  RESULTS
  Quantitated neuropathology worsened with every 10 mm Hg decrement in BP, but the effect of altering PaO2 by 10 mm Hg was not as great, nor as consistent. Autoradiographic study of cerebral blood flow with 14C-iodoantipyrine revealed no hypoxic vasodilatation during ischemia. In the MCA occlusion model, milder hypoxia than in the first series (PaO2 = 46.5 +/- 1.4 mm Hg) exacerbated necrosis to 24.3 +/- 4.7% of the hemisphere from 16.6 +/- 7.0% with normoxia (PaO2 = 120.5 +/- 4.1 mm Hg), whereas hyperoxia (PaO2 = 213.9 +/- 5.8 mm Hg) mitigated hemispheric damage to 7.50 +/- 1.86%. Cortical damage was strikingly sensitive to arterial PaO2, being 12.8 +/- 3.1% of the hemisphere with hypoxia, 7.97 +/-4.63% with normoxia, and only 0.3 +/- 0.2% of the hemisphere with hyperoxia (p < 0.01), and necrosis being eliminated completely in 8 of 10 animals.
  
  
  CONCLUSIONS
  Hypoxia without ischemia does not cause brain necrosis but hypoxia exacerbates ischemic necrosis. Hyperoxia potently mitigates brain damage in this MCA occlusion model, especially in neocortex.
authors.affiliation: Department of Basic Sports Medicine, Kagawa Medical University, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Miyamoto
authors.forename: R N
authors.initials: RN
authors.lastname: Auer
mesh_terms: D000818:Animals; D001284:Atrophy; D001345:Autoradiography; D001794:Blood Pressure; D002540:Cerebral Cortex / Q000098:blood supply / Q000473:pathology*; D002560:Cerebrovascular Circulation; D003128:Coma / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D003342:Corpus Striatum / Q000098:blood supply / Q000473:pathology; D004569:Electroencephalography; D018496:Hyperoxia / Q000150:complications* / Q000473:pathology / Q000503:physiopathology; D000860:Hypoxia / Q000150:complications* / Q000473:pathology / Q000503:physiopathology; D020244:Infarction, Middle Cerebral Artery / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D002546:Ischemic Attack, Transient / Q000150:complications* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D009336:Necrosis; D010100:Oxygen / Q000097:blood; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010100:Oxygen