Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos.
Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate dependent on the efficiency of amplification of the functional light chain gene family by the specific primers. Besides four new functional immunoglobulin variable gene sequences, the isolation of a new pseudogene is reported.
Alcocer
M J
MJ
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
Doyen
C
C
Lee
H A
HA
Morgan
M R
MR
D000595:Amino Acid Sequence; D004390:Chlorpyrifos / Q000276:immunology*; D005818:Genetic Engineering; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology*; D007306:Insecticides / Q000276:immunology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D007306:Insecticides; D004390:Chlorpyrifos