Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis-elements unrelated to canonical translation initiation signals.
In Eubacteria, de novo translation of some internal cistrons may be inefficient or impossible unless the 5' neighboring cistron is also translated (translational coupling). Translation reinitiation is an extreme case of translational coupling in which translation of a message depends entirely on the presence of a nearby terminating ribosome. In this work, the characteristics of mRNA cis-elements inducing the reinitiation process in Escherichia coli have been investigated using a combinatorial approach. A number of novel translational reinitiation sequences (TRSs) were thus identified, which show a wide range of reinitiation activities fully dependent on a translational coupling event and unrelated to the presence/absence of secondary structure or mRNA stability. Moreover, some of the isolated TRSs are similar to intercistronic sequences present in the E. coli genome.
Andrè
A
A
Dipartimento di Genetica, Biologia Generale e Molecolare, Università Federico II di Napoli, via Mezzocannone 8, 80134, Naples, Italy.
Puca
A
A
Sansone
F
F
Brandi
A
A
Antico
G
G
Calogero
R A
RA
D020121:5' Untranslated Regions / Q000235:genetics; D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis; D002474:Cell-Free System / Q000378:metabolism; D018387:Codon, Initiator / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005796:Genes / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D020121:5' Untranslated Regions; D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D018387:Codon, Initiator; D018388:Codon, Terminator; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C461599:maltose transport system, E coli; D005982:Glutathione Transferase; D001616:beta-Galactosidase