Crystallization and preliminary crystal analysis of yeast hexokinase PI and PII.
Hexokinase is the prime enzyme of the Embden-Meyerhof pathway and is responsible for the first stage of energy conversion. It catalyzes the transfer of a phosphate to glucose to form glucose-6-phosphate. Yeast hexokinase PII is also known to play an important role in glucose signal transduction. Crystals of yeast hexokinase isoforms PI and PII were obtained by vapour-diffusion techniques using the hanging-drop method. Isoform PI crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 62.12, b = 78.87, c = 144.74 A. Unit-cell parameters for isoform PII crystals are a = b = 142.81, c = 58.46 A and the space group is I4. Synchrotron diffraction data have been collected to 2.2 A resolution from the isoform PII crystal, whereas isoform PI diffracted to 3.1 A.
Kuser
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PR
Laboratório Nacional de Luz Síncrotron, Caixa Postal 6192, CEP 13083-970, Campinas, SP, Brazil. pkuser@lnls.br
Golubev
A M
AM
Polikarpov
I
I
D003460:Crystallization; D018360:Crystallography, X-Ray; D006593:Hexokinase / Q000737:chemistry* / Q000302:isolation & purification*; D007527:Isoenzymes / Q000737:chemistry* / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D007527:Isoenzymes; D006593:Hexokinase