[Detection of S-gene mutation strain in vertical transmission of HBV and its significance].
OBJECTIVE
To study S-gene mutation of hepatitis B virus (HBV) in its vertical transmission and its significance.


METHODS
Nucleotides of S-gene NT451-660 of HBV were sequenced with dideoxy end termination technique in four female and six male carriers without HBV markers in their spouses and in their intrauterine infected fetuses.


RESULTS
It was showed that homology of HBV nucleotide and amino acid sequences in the mothers, fathers and their fetuses was very high. Mutation at the sites 491, 494, 530, 546 and 581 of S-gene resulted in amino acid substitution at the sites 113, 114, 126, 131 and 143, respectively. Mutations at the sites 126 were detected in two pairs of mother or father and her or his fetuses and mutations at the sites 131 in four fetuses, respectively, including combined mutation at the site 143 in two fetuses.


CONCLUSIONS
Strains with S-gene mutation, mainly at the sites 126, 131 and 143, could be found in HBV vertical transmissions, which could cause failure in HB vaccine immunization.
Wang
S
S
Institute for Military Medicine, Guangzhou.
Jiang
P
P
Peng
G
G
D000328:Adult; D002353:Carrier State; D005260:Female; D014644:Genetic Variation; D006509:Hepatitis B / Q000635:transmission*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D009154:Mutation