title: High-resolution physical map and transcript identification of a prostate cancer deletion interval on 8p22.
abstract: A genomic interval of approximately 1-1.5 Mb centered at the MSR marker on 8p22 has emerged as a possible site for a tumor suppressor gene, based on high rates of allele loss and the presence of a homozygous deletion found in metastatic prostate cancer. The objective of this study was to prepare a bacterial contig of this interval, integrate the contig with radiation hybrid (RH) databases, and use these resources to identify transcription units that might represent the candidate tumor suppressor genes. Here we present a complete bacterial contig across the interval, which was assembled using 22 published and 17 newly originated STSs. The physical map provides twofold or greater coverage over much of the interval, including 17 BACs, 15 P1s, 2 cosmids, and 1 PAC clone. The position of the selected markers across the interval in relation to the other markers on the larger chromosomal scale was confirmed by RH mapping using the Stanford G3 RH panel. Transcribed units within the deletion region were identified by exon amplification, searching of the Human Transcript Map, placement of unmapped expressed sequence tags (ESTs) from the Radiation Hybrid Database (RHdb), and from other published sources, resulting in the isolation of six unique expressed sequences. The transcript map of the deletion interval now includes two known genes (MSR and N33) and six novel ESTs.
authors.affiliation: Section of Hematology-Oncology, Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60607-7170 USA.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Arbieva
authors.forename: K
authors.initials: K
authors.lastname: Banerjee
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: S L
authors.initials: SL
authors.lastname: Edassery
authors.forename: V S
authors.initials: VS
authors.lastname: Maniatis
authors.forename: S K
authors.initials: SK
authors.lastname: Horrigan
authors.forename: C A
authors.initials: CA
authors.lastname: Westbrook
mesh_terms: D002872:Chromosome Deletion; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D005784:Gene Amplification; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects; D008297:Male; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping / Q000379:methods*; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D012333:RNA, Messenger