Lack of interaction in recombinant human FSH receptor and both TSAb and TSBAb.
Since cross-reactivity of TSH with the human FSH receptor has been reported, in this study we tested the effect of thyroid-stimulating antibody (TSAb) and thyroid stimulation-blocking antibody (TSBAb) on Chinese hamster ovary cells expressing human FSH receptor (CHO-hFSH-R cells). We examined the TSBAb activity of sera from hypothyroid patients who had a positive TBII to determine whether these sera also block the effect of FSH on CHO-hFSH-R cells. Although human FSH I-3 (0.25-16 ng/ml) stimulated the production of intracellular cAMP in CHO-hFSH-R cells with dose-responsive manner, neither TSAb nor TSBAb had such an effect on the cells.
Tano
M
M
Department of Obstetrics and Gynecology, School of Medicine, Gunma University, Maebashi, Japan.
Minegishi
T
T
Murakami
M
M
Ibuki
Y
Y
Miyamoto
K
K
D000328:Adult; D000818:Animals; D001323:Autoantibodies / Q000502:physiology; D016466:CHO Cells; D006224:Cricetinae; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006801:Humans; D007037:Hypothyroidism / Q000276:immunology / Q000473:pathology; D018828:Immunoglobulins, Thyroid-Stimulating / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D011962:Receptors, FSH / Q000502:physiology*; D011989:Receptors, Thyrotropin / Q000276:immunology; D011994:Recombinant Proteins
D001323:Autoantibodies; D018828:Immunoglobulins, Thyroid-Stimulating; D011962:Receptors, FSH; D011989:Receptors, Thyrotropin; D011994:Recombinant Proteins; C118905:thyroid stimulation-blocking antibody; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP