Treatment of experimental autoimmune encephalomyelitis with adenylate deaminase from Penicillium lanoso-viride.
The effect of intramuscularly administered immunomodulator, adenylate deaminase (E.C. 3.5.4.6), from Penicillium lanoso-viride on the clinical score of acute experimental autoimmune encephalomyelitis (EAE), a T cell-mediated autoimmune disease, was examined by inoculation of guinea pigs with rabbit brain and spinal cord homogenate (encephalitogen) and complete Freund's adjuvant. Adenylate deaminase (ADA) was effective in delaying the onset of clinical disease. ADA inhibited the severity of EAE. There was a significant decrease in clinical signs. A decrease in the number of morbid and dead animals was observed. Of ADA treated animals, 50-80% developed no clinical manifestations of EAE. The optimal version of treatment was a single preventive injection of ADA 1 day before the sensitization and then every second day after immunization for 20 days. ADA treatment of immunized animals diminished the activity of 2', 3'-cyclic nucleotide 3'-phosphodiesterase in the cerebrospinal fluid, as well the amount of complement fixing antiencephalitogenic antibodies in the blood serum. The mechanism of ADA cerebroprotective action is discussed. Significant skin-allergic cross-reaction of delayed-type hypersensitivity between ADA and encephalitogen was observed.
Nikolajeva
V
V
Department of Microbiology, Faculty of Biology, University of Latvia, 4, Kronvalda boulv., Riga, 1586, Latvia.
Eze
D
D
Petrina
Z
Z
Muiznieks
I
I
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000134:cerebrospinal fluid; D000659:AMP Deaminase / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use*; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use; D000818:Animals; D001921:Brain / Q000276:immunology; D003167:Complement Activation; D003429:Cross Reactions; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000201:enzymology / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007114:Immunization; D007273:Injections, Intramuscular; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010407:Penicillium / Q000201:enzymology; D011817:Rabbits; D012882:Skin Tests; D013116:Spinal Cord / Q000276:immunology
D000276:Adjuvants, Immunologic; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D000659:AMP Deaminase