Cytotoxicity of TNT and its metabolites.
The production and storage of explosives has resulted in the environmental accumulation of 2,4,6-trinitrotoluene (TNT). The biotransformation products of the nitroaromatic compound TNT and metabolites in mammalian cells in culture and their cytotoxicity are studied. We report after our analysis by reverse phase high performance liquid chromatography (HPLC) that the most prevalent biotransformation product of TNT in the NG108 neuroblastoma cells is primarily monoamino-dinitrotoluene (2Am-DNT). It causes toxic effects based on trypan blue exclusion and LDH-release colorimetric assays.
Banerjee
H N
HN
Department of Biology, and Cancer Research Center, Howard University, Washington, DC 20059, USA.
Verma
M
M
Hou
L H
LH
Ashraf
M
M
Dutta
S K
SK
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D009447:Neuroblastoma / Q000188:drug therapy; D018675:Toxicity Tests; D014303:Trinitrotoluene / Q000378:metabolism* / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D014303:Trinitrotoluene