Comparison of oxidative stress indicators in plasma of recent-onset and long-term type 1 diabetic patients.
Oxidative stress was compared in plasma of 15 recently diagnosed (<2 mo) or 15 longstanding (>5 yr) type 1 diabetic patients with 15 healthy volunteers. Lipid peroxidation indices measured in plasma included thiobarbituric acid-reactive substances (TBARS), conjugated dienes, and lipid hydroperoxide (ROOH). The values obtained were corrected for phospholipid to minimize this as a confounding factor. In recently diagnosed diabetics, plasma conjugated lipid dienes were significantly elevated. However, in longstanding diabetics there was a marked increase in TBARS, conjugated dienes, and lipid hydroperoxide levels. Our findings showed increased oxidative stress in type 1 diabetics regardless of metabolic control and that conjugated diene measurement appeared to be the most sensitive bioindicator of oxidant stress in our population.
Güzel
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S
Department of Biochemistry, Cerrahpaşa Medical Faculty, Istanbul University, Turkey.
Seven
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A
Satman
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Burçak
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G
D000328:Adult; D000375:Aging / Q000378:metabolism; D000419:Albuminuria / Q000652:urine; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000150:complications; D003928:Diabetic Nephropathies / Q000097:blood; D003929:Diabetic Neuropathies / Q000097:blood; D003930:Diabetic Retinopathy / Q000097:blood; D005260:Female; D006801:Humans; D008054:Lipid Peroxides / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D010743:Phospholipids / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D008054:Lipid Peroxides; D008055:Lipids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances