Protective effects of Ginkgo biloba extract on cultured rat cardiomyocytes damaged by H2O2.
OBJECTIVE
To investigate the influence of Ginkgo biloba extract (GbE) on cardiomyocytes damaged by H2O2.


METHODS
Cultured rat cardiomyocytes were divided into 3 groups randomly: control group; H2O2 (2.5 mmol.L-1) group; H2O2 2.5 mmol.L-1 + GbE 150 mg.L-1 group. The cardiomyocytes were cultured in MEM (Eagle's) at 37 degrees C in the presence of 5% CO2 for 4 h. Lactate dehydrogenase (LDH) was assayed by colorimetric method. Lipid peroxidation was determined by measuring thiobarbituric acid-reactive substances. Ultrastructure was viewed under transmission electron microscope.


RESULTS
Compared with the control group, LDH leakage and malondialdehyde (MDA) content increased in H2O2 group, LDH increased from (2166 +/- 247) U.L-1 to (5180 +/- 648) U.L-1, MDA increased from (3.5 +/- 0.2) nmol/10(6) cells to (7.2 +/- 0.4) nmol/10(6) cells (P < 0.01). The ultrastructure was damaged seriously. GbE inhibited the increase of LDH leakage and MDA content induced by H2O2. In this group, LDH decreased from (5180 +/- 648) U.L-1 to (3496 +/- 386) U.L-1, MDA decreased from (7.2 +/- 0.4) nmol/10(6) cells to (4.8 +/- 0.9) nmol/10(6) cells (P < 0.01). Ultrastructure of cells was also protected by GbE.


CONCLUSIONS
GbE protected the cardiomyocyte against H2O2 injury, the protective action was attributed to its antiperoxidative effect.
Niu
Y H
YH
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospitol, Shanghai Medical University, China.
Yang
X Y
XY
Bao
W S
WS
D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D010946:Plants, Medicinal; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase