title: Site-specific epithelial-mesenchymal interactions in digestive neuroendocrine tumors. An experimental in vivo and in vitro study.
abstract: Little is known about the functional interactions between digestive neuroendocrine tumor cells and their stromal microenvironment. The focus of our study is whether mesenchymal cells modulate peptide expression, cell proliferation, and invasiveness in digestive neuroendocrine tumor cells. We designed an experimental in vivo and in vitro study using the mouse enteroendocrine cell line STC-1. In vivo, STC-1 cells were injected subcutaneously in 18 immunosuppressed newborn rats. At day 21, all animals presented poorly differentiated neuroendocrine tumors with lung metastases. Subcutaneous tumors were usually limited by a capsule containing basement membrane components and myofibroblasts that presented a low mitotic index. Lung tumors were devoid of capsule and poor in myofibroblasts, and their mitotic index was high. The profile of peptide expression in STC-1 tumors was different from that of cultured STC-1 cells. In vitro, STC-1 cells were cultured with fibroblasts of different origins, including dermis, lung, digestive tract, and liver. Based on their origin, myofibroblasts differentially modulated hormone synthesis, proliferation, spreading, and adhesion of STC-1 cells. In conclusion, our results show that site-specific functional interactions between mesenchymal and neuroendocrine cells may contribute to modulating the behavior of digestive neuroendocrine tumors, depending on their growth site.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité-45, Lyon France.
authors.forename: J
authors.initials: J
authors.lastname: Dumortier
authors.forename: C
authors.initials: C
authors.lastname: Ratineau
authors.forename: J Y
authors.initials: JY
authors.lastname: Scoazec
authors.forename: C
authors.initials: C
authors.lastname: Pourreyron
authors.forename: W
authors.initials: W
authors.lastname: Anderson
authors.forename: M F
authors.initials: MF
authors.lastname: Jacquier
authors.forename: M
authors.initials: M
authors.lastname: Blanc
authors.forename: C
authors.initials: C
authors.lastname: Bernard
authors.forename: C
authors.initials: C
authors.lastname: Bellaton
authors.forename: L
authors.initials: L
authors.lastname: Remy
authors.forename: J A
authors.initials: JA
authors.lastname: Chayvialle
authors.forename: C
authors.initials: C
authors.lastname: Roche
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division; D002460:Cell Line / Q000378:metabolism; D004067:Digestive System Neoplasms / Q000503:physiopathology*; D004701:Endocrine Gland Neoplasms / Q000503:physiopathology*; D004848:Epithelium / Q000503:physiopathology; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D006728:Hormones / Q000378:metabolism; D008175:Lung Neoplasms / Q000473:pathology; D008648:Mesoderm / Q000502:physiology; D051379:Mice; D009361:Neoplasm Invasiveness / Q000473:pathology; D009423:Nervous System Neoplasms / Q000503:physiopathology*; D010455:Peptides / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D051381:Rats; D017208:Rats, Wistar; D012878:Skin Neoplasms / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D006728:Hormones; D010455:Peptides; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D014157:Transcription Factors