Analysis of splicing of four mouse JNK/SAPKalpha variants.
The JNK/SAPK (c-Jun NH2-terminal kinase/stress-activated protein kinase) cascade is activated by a variety of stress stimuli and by the inflammatory cytokines interleukin-I (IL-I) and tumor necrosis factor alpha (TNFalpha). Four splice variants of the mouse JNK/SAPKalpha isoform, which differ in a region located in subdomains IX-X of the protein, were previously identified. Analysis of the sequence of the central region of the mouse JNK/SAPKalpha gene indicates that splice variants I and II are generated by a typical alternative splicing mechanism, while splice variants III and IV are generated by a less common mechanism, where alternative 3' splice sites located inside an exon (cryptic sites) are selected. The major splice variants alphaI and all have a wide and similar distribution in hippocampus, cerebral cortex, caudate-putamen, amygdala and the granule cell layer of cerebellum, although their expression is specifically regulated in certain cell types.
Casanova
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Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
Callejo
A I
AI
Calvo
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Chinchetru
M A
MA
D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000679:Amygdala / Q000201:enzymology; D000818:Animals; D001483:Base Sequence; D002421:Caudate Nucleus / Q000201:enzymology; D002531:Cerebellum / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D006624:Hippocampus / Q000201:enzymology; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics; D051379:Mice; D048056:Mitogen-Activated Protein Kinase 9; D020928:Mitogen-Activated Protein Kinases; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D011699:Putamen / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D011494:Protein Kinases; D048056:Mitogen-Activated Protein Kinase 9; D020928:Mitogen-Activated Protein Kinases