High-throughput and ultra-high-throughput screening: solution- and cell-based approaches.
The trend towards assay miniaturization for high-throughput and ultra-high-throughput screening continues to spur development of homogeneous, fluorescence-based assays in higher density, smaller volume microplate formats. Recently, first-generation microfluidic devices have been designed for performing continuous-flow biochemical and cell-based assays. These devices provide orders-of-magnitude reduction in reagent consumption, and offer the potential for implementing high-throughput screening in formats that integrate up-front compound handling with unique assay functionality.
Sundberg
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Caliper Technologies Corporation, CA 94043, USA. steve. sundberg@calipertech.com
D000818:Animals; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D002477:Cells / Q000201:enzymology / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004798:Enzymes / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D008024:Ligands; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods; D011485:Protein Binding; D012996:Solutions
D004798:Enzymes; D008024:Ligands; D012996:Solutions