Inhibitory effects of tea extracts on the mutagenicity of 1-methyl-1, 2,3,4-tetrahydro-beta-carboline-3-carboxylic acid on treatment with nitrite in the presence of ethanol.
It has been shown that the mutagenicity of 1-methyl-1,2,3, 4-tetrahydro-beta-carboline-3-carboxylic acid (MTCCA), a major mutagen precursor in soy sauce on treatment with nitrite and ethanol, was strongly decreased by the addition of hot water extracts of green, black and oolong teas in the reaction mixture when it was treated with 50mM nitrite at pH3.0, 37 degrees C for 60min in the presence of 7.5% ethanol. The mutagenicity-decreasing activity of the teas was scarcely decreased by washing the teas with chloroform and benzene and was partly decreased by butanol and ethyl acetate. Typical polyphenols such as catechins were shown to have the antimutagenicity dose dependently. The antimutagenicity and the reducing power of tea extracts gave a positive good correlation. The results suggest that the mutagenicity of MTCCA on treatment with nitrite in the presence of ethanol may be decreased by the mixed fractions of lyophilic components such as polyphenols, which have high reducing power such as catechins and the other compounds which have little reducing power including the derivatives of the catechins and so on. Although the antimutagenicity of teas and catechins was also considerably effective when they were added after the nitrosation, that of black tea and some catechins was less effective.
Higashimoto
M
M
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima, Japan.
Akada
Y
Y
Sato
M
M
Kinouchi
T
T
Kuwahara
T
T
Ohnishi
Y
Y
D002243:Carbolines / Q000493:pharmacokinetics / Q000494:pharmacology / Q000633:toxicity*; D002392:Catechin / Q000378:metabolism / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D000431:Ethanol / Q000494:pharmacology; D009152:Mutagenicity Tests; D009573:Nitrites / Q000494:pharmacology; D010084:Oxidation-Reduction; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013025:Glycine max / Q000737:chemistry; D013662:Tea / Q000737:chemistry*
D002243:Carbolines; D009573:Nitrites; D013662:Tea; D000431:Ethanol; C052025:1,2,3,4-tetrahydro-1-methyl-beta-carboline-1-carboxylic acid; D002392:Catechin