[Oxidative DNA damage of cartilage in osteoarthrosis].
In order to study molecular mechanism of osteoarthrosis, we used molecular techniques to detect DNA oxidative damage of cartilage. There were 10 samples from clinical operation including 5 cases of replacement of hip joint, 4 degenerative cartilage of knee osteoarthrosis, and 1 free body of knee joint. DNA from all samples was run out on an agarose gel and appeared classic nucleosomal ladders. (8-hydroxyl-2'-deoxyguanosine)/ml (8-OHdG) in DNA samples was measured by high-performance liquid chromatography with electrochemical detection (HPLC-EC). The results showed that the levels of 8-OHdG were increased to 0.11-0.26 ng.ml-1, average 0.177 ng.ml-1. Normal controls were lower than 0.05 ng.ml-1. The high 8-OHdG in DNA led to a misreading of affected templates and base-pair exchange, which could be a sensitive indicator about oxidative DNA damage, which was produced by free radicals or other DNA damage agents. The result suggests that oxidative DNA damage may be the molecular mechanism of osteoarthrosis.
Zhu
J
J
Department of Rehabilitation Medicine, Xiangya Hospital, Hunan Medical University, Changsha.
Yue
Z
Z
Wang
J
J
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D017209:Apoptosis; D015415:Biomarkers; D002358:Cartilage, Articular / Q000378:metabolism*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000209:etiology / Q000235:genetics*
D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine