[Maturation of bone marrow megakaryocyte in patients with chronic idiopathic thrombocytopenic purpura].
Colony formation units of megakaryocyte of twenty four patients with idopathic thromboeytopentic purpura (CITP) were observed by plasma clot cultures in vitro. We observed the maturation degree of megakarycyte and the effect of recombinant interferon alpha-2a (rIFN-alpha-2a) on megakaryocyte colony growth and maturation. The results showed that the number of CFU-MK of the patients with CITP was greater than that of the control group, while the patients with normal number of megakaryocytes on bone marrow smears had less BFU-MK and total clonies than the controls had. Through image analysis, we found that the black level of the positive cells of GP II a and GMP-140, the diameter and the area of megakaryocyte of patients with CITP were lower or smaller than those of the control group. These evidenced the block of megakaryocytopoiesis and megakarycyte maturation. The growth of MK colonies of marrow of patients with CITP was inhibited by r-IFN-alpha-2a.
Wei
J
J
Department of Internal Medicine, First Affiliated Hospital, Chengdu.
Deng
C
C
Meng
W
W
Niu
T
T
D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000187:drug effects / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002908:Chronic Disease; D003114:Colony-Forming Units Assay; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology; D008297:Male; D008533:Megakaryocytes / Q000187:drug effects / Q000473:pathology*; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000473:pathology*; D011994:Recombinant Proteins
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins