title: Effects of antibiotics on adherence of Pseudomonas aeruginosa and Pseudomonas fluorescens to A549 pneumocyte cells.
abstract: The aim of this study was to evaluate the effect of antibiotics at subminimal inhibitory concentrations (sub-MIC) on fluorescent pseudomonas adherence to A549 pneumocyte cells. Pseudomonas fluorescens MF0 isolated from contaminated raw milk and Pseudomonas aeruginosa NK125502 isolated from a cystic fibrosis patient's lung adhered to A549 cells. As previously shown for P. aeruginosa, P. fluorescens bound to A549 cells in a dose-dependent manner over a wide range of bacterial concentrations. Bacterial growth in the presence of polymyxin B or gentamicin at MIC/2 had no effect on the adherence of NK125502 and MF0 to A549 cells. Instead, MIC/2 and MIC/8 of cefsulodin or chloramphenicol decreased the adherence of the two strains. A decrease in MF0 adherence was also observed with cefsulodin at MIC/32. We conclude that, in addition to their antibacterial activity, cefsulodin and chloramphenicol could be effective in preventing Pseudomonas adherence to respiratory epithelium.
authors.affiliation: Laboratoire ERRMECe, Equipe Microbiologie, Université de Cergy-Pontoise, France. martino@paris.u-cergy.fr
authors.forename: P
authors.initials: P
authors.lastname: Di Martino
authors.forename: J
authors.initials: J
authors.lastname: Rebière-Huët
authors.forename: C
authors.initials: C
authors.lastname: Hulen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002441:Cefsulodin / Q000494:pharmacology; D002460:Cell Line; D002701:Chloramphenicol / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects* / Q000382:microbiology*; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D011112:Polymyxin B / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D011551:Pseudomonas fluorescens / Q000187:drug effects* / Q000502:physiology; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D002701:Chloramphenicol; D011112:Polymyxin B; D002441:Cefsulodin