Polycations as displacer in high-performance bioseparation.
Displacement chromatography is an interesting but up to now rarely used type of preparative biochromatography. The lack of well-engineered and accessible displacer contributes to this phenomenon. In this paper a novel type of displacer is introduced for cation-exchange displacement chromatography, which will soon become commercially available. The molecule is a well-defined PolyDADMAC [poly(diallyldimethylammonium chloride)] with a molar mass of less than 35000 g/mol, an exclusively linear structure and a molar mass polydispersity of less than 1.5. A method for synthesizing such a polymer at high yields is described. The PolyDADMAC is shown to be an efficient displacer of basic proteins from strong cation-exchange columns.
Schmidt
B
B
Institute of Chemical Engineering, Swiss Federal Institute of Technology Lausanne, Ecublens, Switzerland.
Wandrey
C
C
Vogt
S
S
Freitag
R
R
Holzapfel
H
H
D002852:Chromatography, Ion Exchange / Q000379:methods*; D003574:Cytochrome c Group / Q000302:isolation & purification; D008970:Molecular Weight; D009113:Muramidase / Q000302:isolation & purification; D011073:Polyamines; D000071228:Polyelectrolytes; D011095:Polyethylenes / Q000138:chemical synthesis / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis / Q000737:chemistry
D003574:Cytochrome c Group; D009113:Muramidase; D011073:Polyamines; D000071228:Polyelectrolytes; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride