title: Crystallization of the N-terminal domain of human sex hormone-binding globulin, the major sex steroid carrier in blood.
abstract: The amino-teminal laminin G-like domain of human sex hormone-binding globulin (SHBG), which contains the steroid-binding site and the dimerization domain, has been produced in Escherichia coli, purified to homogeneity and crystallized in complex with 5alpha--dihydrotestosterone (DHT) in two different crystal forms. Native data sets have been collected for tetragonal crystals (space group P4(1)22 or P4(3)22; unit-cell parameters a = 52.2, c = 148.4 A) diffracting to 3.3 A and trigonal crystals (R32; a = 104.0, c = 84.4 A) diffracting to better than 1.6 A. Since both crystal forms can only accommodate a single monomer in the asymmetric unit and share twofold rotational symmetry, it is proposed that the homodimer of this truncated form of SHBG, as observed in ultracentrifugation experiments, displays C(2) point-group symmetry.
authors.affiliation: Forschungsgruppe Kristallographie, Max-Delbrück-Center for Molecular Medicine, 13092 Berlin, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Grishkovskaya
authors.forename: G
authors.initials: G
authors.lastname: Sklenar
authors.forename: G V
authors.initials: GV
authors.lastname: Avvakumov
authors.forename: D
authors.initials: D
authors.lastname: Dales
authors.forename: J
authors.initials: J
authors.lastname: Behlke
authors.forename: G L
authors.initials: GL
authors.lastname: Hammond
authors.forename: Y A
authors.initials: YA
authors.lastname: Muller
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012738:Sex Hormone-Binding Globulin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D012738:Sex Hormone-Binding Globulin