title: Purification of the Ca2+-binding protein S100A1 from myocardium and recombinant Escherichia coli.
abstract: S100A1 is a new regulatory protein of myocardial contractility that is differentially expressed in early and late stages of myocardial hypertrophy. In order to further investigate the multiple functions of S100A1 in various assay systems we developed a new strategy for isolating biologically active S100A1 protein. After EDTA extraction of myocardium or recombinant bacteria, S100A1 was purified by Octyl-Sepharose hydrophobic interaction chromatography and HiTrapQ anion-exchange chromatography yielding 1.4-2.0 mg/100 g wet tissue and 0.7-1.0 mg/100 ml bacterial culture. Native porcine as well as human recombinant S100A1 revealed biological activity in physiological and biochemical assays.
authors.affiliation: Department of Medicine II, Medical University of Lübeck, Germany. ehlerman@medinf.mu-luebeck.de
authors.forename: P
authors.initials: P
authors.lastname: Ehlerman
authors.forename: A
authors.initials: A
authors.lastname: Remppis
authors.forename: P
authors.initials: P
authors.lastname: Most
authors.forename: J
authors.initials: J
authors.lastname: Bernotat
authors.forename: C W
authors.initials: CW
authors.lastname: Heizmann
authors.forename: H A
authors.initials: HA
authors.lastname: Katus
mesh_terms: D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D009206:Myocardium / Q000737:chemistry*; D011994:Recombinant Proteins / Q000302:isolation & purification; D009418:S100 Proteins
chemical_list: D002135:Calcium-Binding Proteins; D011994:Recombinant Proteins; D009418:S100 Proteins; C110294:S100A1 protein