title: The synergistic effect of thrombopoietin in erythropoiesis and myelopoiesis from human bone marrow cells.
abstract: We sought to determine whether recombinant human thrombopoietin (TPO) acts synergistically with recombinant human erythropoietin (EPO) and/or recombinant human interleukin-3 (IL-3) on erythroid burst formation and granulocyte-macrophage colony formation from human bone marrow (BM). BM cells were from 5 adults and 15 children who underwent bone marrow examination because of a clinical suspicion of malignancy; their bone marrows as well as the complete blood counts were normal and were cultured in a methylcellulose system. TPO has a synergistic effect with EPO or EPO + IL-3 on erythropoiesis of human BM, as the addition of TPO to EPO significantly gave rise to more erythroid bursts (p = 0.0001) and the addition of TPO to EPO + IL-3 might give rise to more erythroid bursts (p = 0.05). TPO also has a synergistic effect with recombinant human granulocyte colony-stimulating factor (G-CSF) on myelopoiesis of human BM, since the addition of TPO to G-CSF gave rise to significantly more granulocyte-macrophage colonies (p = 0. 0001). Besides its well-known significant role in megakaryopoiesis, TPO also has synergistic effects on erythropoiesis and myelopoiesis.
authors.affiliation: Department of Pediatrics, Mackay Memorial Hospital, Taipei, Taiwan. dcliang@ms2.mmh.org.tw
authors.forename: D
authors.initials: D
authors.lastname: Liang
authors.forename: L Y
authors.initials: LY
authors.lastname: Shih
authors.forename: M C
authors.initials: MC
authors.lastname: Kuo
authors.forename: S H
authors.initials: SH
authors.lastname: Chen
authors.forename: H C
authors.initials: HC
authors.lastname: Liu
authors.forename: A
authors.initials: A
authors.lastname: Shimosaka
mesh_terms: D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D019891:Leukopoiesis / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology*
chemical_list: D007377:Interleukin-3; D011994:Recombinant Proteins; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D013926:Thrombopoietin