title: Distribution of actin bundles in Bowman's capsule of rat kidney.
abstract: In this study we define the distribution of actin bundle arrangement in Bowman's capsule of rat renal corpuscles. Parietal cells of Bowman's capsule were examined by conventional light microscopy, electron microscopy and confocal microscopy. Within each parietal cell individual actin bundles are arranged in a parallel fashion running the length of the cell. Computer reconstructions obtained using confocal microscopy clearly show the lengths of actin bundles to be arranged, on a capsule level, end-to-end, at angles and perpendicular to bundles in adjacent cells. The bundles stain positively for non-muscle myosin and vinculin. The presence and arrangement of actin bundles in parietal cells is consistent with a role in reinforcing capsule structure.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Vancouver, Canada. djm@interchange.ubc.ca
authors.forename: D J
authors.initials: DJ
authors.lastname: Mulholland
authors.forename: J
authors.initials: J
authors.lastname: Wilson
authors.forename: W
authors.initials: W
authors.lastname: Vogl
authors.forename: W
authors.initials: W
authors.lastname: Webber
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009218:Myosins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D016596:Vinculin; D009218:Myosins