Histomorphological and histochemical alterations following short-term inhalation exposure to sulfur mustard on visceral organs of mice.
Toxic effects of inhaled sulfur mustard (SM) on the histology of visceral organs was investigated by exposing mice to 84.6 mg/m3 for 1 h duration, using controlled single exposure conditions. A progressive fall in body weight from third day onwards was noticed. Light microscopic examination of the pulmonary tissue of these animals at 6 h post exposure revealed that the tracheobronchial epithelium remained intact, but was infiltrated by inflammatory cells. By 24 h post exposure, the mucosecretory cells were destroyed. The inflammatory reaction was maximum at 48 h. By 7th day post exposure there was swelling and vacuolation of lung parenchymal cells and thrombi formation. In addition SM caused congestion and hemorrhage at alveolar level. SM also caused granulovacuolar degeneration with perinuclear clumping of the cytoplasm of hepatocytes and renal parenchymal cells. Renal lesions were characterized by congestion and hemorrhage. Among visceral tissues, maximum atrophy was observed in spleen. Distribution of lesions increased with post exposure period. The maximum lesions were observed at 7th day post-exposure.
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Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India.
Vijayaraghavan
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D000818:Animals; D003879:Dermatologic Agents / Q000633:toxicity*; D005260:Female; D006470:Hemorrhage / Q000209:etiology; D019570:Inhalation Exposure; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D051379:Mice; D009151:Mustard Gas / Q000633:toxicity*; D013997:Time Factors; D015431:Weight Loss
D003879:Dermatologic Agents; D009151:Mustard Gas