Fragile X (fmr1) mRNA expression is differentially regulated in two adult models of activity-dependent gene expression.
We sought to determine whether the fragile X mental retardation gene fmr1 is regulated in long-term potentiation (LTP) and electroconvulsive shock (ECS). In situ hybridization of fmr1 mRNA in hippocampus of rats given LTP in vivo showed no change in fmr1 mRNA levels relative to control. However, ECS induced a selective increase in fmr1 mRNA expression in the dentate gyrus (DG) granule cell layer at 6 h post-ECS. The ECS paradigm may unmask relevant activity-dependent regulatory mechanisms that modulate fmr1 gene transcription in vivo.
Valentine
G
G
Section on Functional Neuroanatomy, Bldg. 36, Rm. 2D15, National Institute of Mental Health, Bethesda, MD, USA.
Chakravarty
S
S
Sarvey
J
J
Bramham
C
C
Herkenham
M
M
D000704:Analysis of Variance; D000818:Animals; D018891:Dentate Gyrus / Q000737:chemistry / Q000378:metabolism*; D004597:Electroshock; D051860:Fragile X Messenger Ribonucleoprotein 1; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D017774:Long-Term Potentiation / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D016601:RNA-Binding Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley
D051860:Fragile X Messenger Ribonucleoprotein 1; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C497685:Fmr1 protein, rat