Preparation of biodegradable microspheres of testosterone with poly(D,L-lactide-co-glycolide) and test of drug release in vitro.
Biodegradable microspheres formulation of testosterone (T) can be used as a new physiological approach for androgen replacement in hypogonadal men. In this study, poly(D,L-lactide-co-glycolide) (PLGA) microspheres containing T were prepared by a solvent-evaporation/solvent-diffusion process and the drug release tests of the microspheres were carried out in vitro. T/PLGA microspheres with good yield, desired size and satisfied drug loading were obtained. A significant testosterone sustained release was shown in the drug release tests in vitro. Since PLGA microspheres preparations are normally sterilized by colbat-60 irradiation, the effects of 25 kGy colbat-60 irradiation on physicochemical properties and in vitro drug release profile of T/PLGA microsphere were investigated. The results showed that the irradiation didn't have any effects on the physicochemical properties of T. Though about one-third decrease in molecular weight of PLGA was caused by the irradiation, no significant changes were observed on the drug release profile in vitro.
Shen
Z R
ZR
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou, China.
Zhu
J H
JH
Ma
Z
Z
Wang
F
F
Wang
Z Y
ZY
D001673:Biodegradation, Environmental; D003037:Cobalt Radioisotopes / Q000494:pharmacology; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000493:pharmacokinetics / Q000528:radiation effects; D004339:Drug Compounding; D019344:Lactic Acid / Q000528:radiation effects; D008863:Microspheres; D011100:Polyglycolic Acid / Q000528:radiation effects; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000528:radiation effects; D013739:Testosterone / Q000378:metabolism / Q000493:pharmacokinetics* / Q000528:radiation effects
D003037:Cobalt Radioisotopes; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D013739:Testosterone