title: Sequence variations in the Boophilus microplus Bm86 locus and implications for immunoprotection in cattle vaccinated with this antigen.
abstract: Cattle tick infestations constitute a major problem for the cattle industry in tropical and subtropical regions of the world. Traditional control methods have been only partially successful, hampered by the selection of chemical-resistant tick populations. The Boophilus microplus Bm86 protein was isolated from tick gut epithelial cells and shown to induce a protective response against tick infestations in vaccinated cattle. Vaccine preparations including the recombinant Bm86 are used to control cattle tick infestations in the field as an alternative measure to reduce the losses produced by this ectoparasite. The principle for the immunological control of tick infestations relies on a polyclonal antibody response against the target antigen and, therefore, should be difficult to select for tick-resistant populations. However, sequence variations in the Bm86 locus, among other factors, could affect the effectiveness of Bm86-containing vaccines. In the present study we have addressed this issue, employing data obtained with B. microplus strains from Australia, Mexico, Cuba, Argentina and Venezuela. The results showed a tendency in the inverse correlation between the efficacy of the vaccination with Bm86 and the sequence variations in the Bm86 locus (R2 = 0.7). The mutation fixation index in the Bm86 locus was calculated and shown to be between 0.02 and 0.1 amino acids per year. Possible implications of these findings for the immunoprotection of cattle against tick infestations employing the Bm86 antigen are discussed.
authors.affiliation: Mammalian Cell Genetics Division, Centro de Ingeniería Genética y Biotecnología, Havana, Cuba.
authors.forename: J C
authors.initials: JC
authors.lastname: García-García
authors.forename: I L
authors.initials: IL
authors.lastname: Gonzalez
authors.forename: D M
authors.initials: DM
authors.lastname: González
authors.forename: M
authors.initials: M
authors.lastname: Valdés
authors.forename: L
authors.initials: L
authors.lastname: Méndez
authors.forename: J
authors.initials: J
authors.lastname: Lamberti
authors.forename: B
authors.initials: B
authors.lastname: D'Agostino
authors.forename: D
authors.initials: D
authors.lastname: Citroni
authors.forename: H
authors.initials: H
authors.lastname: Fragoso
authors.forename: M
authors.initials: M
authors.lastname: Ortiz
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: de la Fuente
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000276:immunology; D014644:Genetic Variation; D018884:Ixodes / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins; D013984:Tick Infestations / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary; D014612:Vaccines; D014614:Vaccines, Synthetic
chemical_list: C085065:BM86 protein, Boophilus; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines; D014614:Vaccines, Synthetic