title: Novel missense mutations that affect the transport function of MalK, the ATP-binding-cassette subunit of the Salmonella enterica serovar typhimurium maltose transport system.
abstract: We report on novel mutations in the malK gene of Salmonella enterica serovar Typhimurium, encoding the ATPase subunit of the maltose transporter (MalFGK(2)). Biochemical analysis suggests that (i) L86 might be involved in a signaling step during substrate translocation and (ii) E306 may be critical for the structural integrity of the protein.
authors.affiliation: Institut für Biologie/Bakterienphysiologie, Humboldt-Universität zu Berlin, D-10099 Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Hunke
authors.forename: H
authors.initials: H
authors.lastname: Landmesser
authors.forename: E
authors.initials: E
authors.lastname: Schneider
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D014644:Genetic Variation; D008081:Liposomes / Q000378:metabolism; D008320:Maltose / Q000378:metabolism*; D016296:Mutagenesis; D009154:Mutation; D020125:Mutation, Missense; D017434:Protein Structure, Tertiary; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D008081:Liposomes; C068021:MalK protein, Bacteria; C489399:MalK protein, Salmonella typhimurium; D008320:Maltose; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases