title: Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing.
abstract: Either of the first two introns of the Arabidopsis tryptophan pathway gene PAT1 elevates mRNA accumulation from a PAT1:beta-glucuronidase (GUS) fusion roughly 5-fold without affecting the rate of PAT1:GUS transcription. To further explore the mechanism of this intron-mediated enhancement of gene expression, we wanted to determine whether splicing or specific intron sequences were necessary. In-frame derivatives of PAT1 intron 1, whose splicing was prevented by a point mutation or large deletions, were able to increase mRNA accumulation from a PAT1:GUS fusion, demonstrating that splicing per se is not required. Furthermore, each of a series of introns containing overlapping deletions that together span PAT1 intron 1 increased PAT1:GUS mRNA accumulation as much as the full-length intron did, indicating that all intron sequences are individually dispensable for this phenomenon. These results eliminate the simple idea that this intron stimulates mRNA accumulation via a unique RNA-stabilizing sequence or through the completed act of splicing. However, they are consistent with a possible role for redundant intron sequence elements or an association of the pre-mRNA with the spliceosome.
authors.affiliation: Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA. abrose@ucdavis.edu
authors.forename: A B
authors.initials: AB
authors.lastname: Rose
authors.forename: J A
authors.initials: JA
authors.lastname: Beliakoff
mesh_terms: D001483:Base Sequence; D018744:DNA, Plant; D005786:Gene Expression Regulation / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing
chemical_list: D018744:DNA, Plant