title: Effects of vitamins A and E on superoxide production and intracellular signaling of neutrophils in Holstein calves.
abstract: The effects of vitamin A and vitamin E treatment on superoxide (O2-) production of neutrophils and their intracellular signaling, including intracellular Ca2+, protein kinase C (PKC), and tyrosine kinase (TK), were examined in Holstein calves. After treatment with vitamin A, heat-aggregated IgG (H-agg.IgG)-induced O2- production in neutrophils ranged from 3.7+/-0.4 to 4.3+/-0.9 nmol (mean +/- SD), and it was significantly (P<0.05) higher than that in the control calves. Opsonized zymosan (OPZ)-induced O2- production was similar with that of control calves. Intracellular signaling of neutrophils in vitamin A-treated calves was enhanced compared with that of control calves when stimulated with H-agg. IgG, but not with OPZ. After treatment with vitamin E, H-agg.IgG- and OPZ-induced O2- production of neutrophils ranged from 4.2+/-0.8 nmol to 5.4+/-0.5 nmol and from 7.8+/-0.6 nmol to 8.1+/-0.4 nmol (mean +/- SD), respectively, and they were significantly (P<0.05) higher than those in control calves. Intracellular signaling was also enhanced compared with that of control calves. These results suggested that the effects of vitamin A and E treatment on O2- production were correlated to changes in intracellular signaling of neutrophils in Holstein calves.
authors.affiliation: Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan. higuchi@rakuno.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Higuchi
authors.forename: H
authors.initials: H
authors.lastname: Nagahata
mesh_terms: D000818:Animals; D015220:Calcium Channels; D002417:Cattle / Q000502:physiology*; D002450:Cell Communication; D009504:Neutrophils / Q000502:physiology*; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction; D013481:Superoxides / Q000378:metabolism*; D014801:Vitamin A / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D015220:Calcium Channels; D013481:Superoxides; D014801:Vitamin A; D014810:Vitamin E; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C