Macropinocytosis as a mechanism of entry into primary human urethral epithelial cells by Neisseria gonorrhoeae.
Gonococcal entry into primary human urethral epithelial cells (HUEC) can occur by macropinocytosis. Scanning and transmission electron microscopy revealed lamellipodia surrounding gonococci, and confocal laser scanning microscopy analysis showed organisms colocalized with M(r) 70,000 fluorescein isothiocyanate-labeled dextran within the cells. Phosphoinositide 3-kinase inhibitors and an actin polymerization inhibitor prevented macropinocytic entry of gonococci into HUEC.
Zenni
M K
MK
Department of Urology, University of Iowa, Iowa City, Iowa 52242, USA.
Giardina
P C
PC
Harvey
H A
HA
Shao
J
J
Ketterer
M R
MR
Lubaroff
D M
DM
Williams
R D
RD
Apicella
M A
MA
D000199:Actins / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009344:Neisseria gonorrhoeae / Q000502:physiology*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010873:Pinocytosis; D014521:Urethra / Q000382:microbiology*
D000199:Actins; D000081082:Phosphoinositide-3 Kinase Inhibitors