Effects of long-term sub-lethal concentrations of dental monomers on THP-1 human monocytes.
Studies have shown that monomers from dental resins are acutely cytotoxic, but little is known of their long-term effects at sub-lethal concentrations. The current study determined the long-term effects of sub-lethal concentrations of TEGDMA (triethyleneglycol dimethacrylate) and Bis-GMA (bisphenol-glycidylinethacrylate), two common dental monomers, on the in vitro cellular proliferation, succinic dehydrogenase activity, and total cellular protein production of monocytes. Human THP-1 monocytes were exposed to concentrations of 100, 200, and 400 micromol l(-1) of TEGDMA or 1, 5, and 25 micromol l(-1) Bis-GMA for 5 weeks. Controls received only vehicle solutions of ethanol. Each week cellular proliferation (hemocytometer), succinic dehydrogenase (SDH) activity (MTT) and total cellular protein (bicinchoninic acid) were assessed. The results were compared with ANOVA and Tukey intervals (alpha = 0.05). TEDGMA had no proliferative or cellular protein effects, but increased SDH activity 20-60% in week 1 (p < 0.05). SDH activity then decreased 40% in week 2, followed by a gradual increase of 30-40% over week 3-5 (p < 0.05). Bis-GMA reduced proliferation by 40-60% from 1-5 weeks exposure (p < 0.05). However, SDH activity and total protein per cell were not affected. There was some indication of increased SDH activity after 5 weeks (20-30%, p < 0.05). Sub-lethal concentrations of TEGDMA and Bis-GMA have significant long-term effects on monocytes at low-dose 5-week exposures in vitro. Each monomer acted differently.
Lefebvre
C A
CA
Department of Oral Rehabilitation, Medical College of Georgia, School of Dentistry, Augusta, USA. clefebvr@mail.mcg.edu
Wataha
J C
JC
Bouillaguet
S
S
Lockwood
P E
PE
D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology / Q000633:toxicity; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003188:Composite Resins / Q000633:toxicity*; D003764:Dental Materials / Q000633:toxicity*; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010452:Peptide Biosynthesis / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology / Q000633:toxicity; D011109:Polymethacrylic Acids / Q000494:pharmacology / Q000633:toxicity; D013385:Succinate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D003188:Composite Resins; D003764:Dental Materials; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; D013385:Succinate Dehydrogenase