Efficacy of further attempts to mobilize CD34+ peripheral stem cells with alternative procedures after primary failure.
19 patients who failed the target collection of at least 2.5 x 10(6) CD34+ cells/kg underwent further mobilization procedures either with granulocyte-colony-stimulating factor (G-CSF) alone after failure to chemotherapy plus G-CSF (group 1), or with chemotherapy plus G-CSF (group 2), or with high-dose G-CSF (24 microg/kg) alone (group 3) after failure to respond to standard dose of G-CSF (10 microg/kg) alone. In all groups, an increase in median CD34+ cell yield could be observed following alternative procedures (1.1- to 1.9 x 10(6) kg; p = 0.02). The highest increase in CD34+ cell harvest was achieved in group 1 (0.85 to 2.2 x 10(6) kg), followed by group 2 (1. 2 to 1.7) and group 3 (1.0 to 1.4), but without statistically significant difference between the mobilization technologies. All patients with more than 1.0 x 10(6) CD34+ cells/kg in the first apheresis procedure reached the overall target of 2.5 x 10(6) CD34+ cells/kg after a second or subsequent mobilization procedure. In contrast, only 3 of 8 patients (37%) with less than 1.0 x 10(6) CD34+ cells in the first harvest could reach the target of 2.5 x 10(6) CD34+ cells after further mobilization attempts.
Kröger
N
N
Bone Marrow Transplantation Center, University Hospital Hamburg-Eppendorf, Hamburg, Germany.
Rauhöft
C
C
Zeller
W
W
Renges
H
H
Hassan
H T
HT
Krüger
W
W
Schuch
G
G
Wacker-Backhaus
G
G
Colberg
H
H
Kuse
R
R
Gutensohn
K
K
Löliger
C
C
Zander
A R
AR
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D002452:Cell Count; D002465:Cell Movement; D002648:Child; D016179:Granulocyte Colony-Stimulating Factor; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D013234:Stem Cells / Q000276:immunology
D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor