Changes of the thromboxane A2/prostacyclin balance in the urine of patients with renal diseases analyzed by gas chromatography/selected ion monitoring.
The thromboxane A2/prostacyclin (TX/PGI) ratios were measured in patients with renal diseases to elucidate the relationship between the ratios and the pathological changes of the diseases. Urinary stable metabolites of thromboxane A2 and prostacyclin, 11-dehydro-thromboxane B2 and 2,3-dinor-6-keto-prostaglandin F1alpha, respectively, were converted to 1-methyl ester-propylamide-9,12,15-tris-dimethylisopropylsilyl ether derivative and 1-methyl ester-6-methoxime-9,12,15-tris-dimethylisopropylsilyl ether derivative, respectively, and applied to a gas chromatography/selected ion monitoring. The TX/PGI ratios of 10 outpatients and 6 inpatients with chronic glomerulonephritis were higher than those of 13 healthy volunteers. In an inpatient with systemic lupus erythematoides, the TX/PGI ratios were gradually lowered to the normal level with the therapies. Furthermore, the ratios seemed to change in advance of the changes of the levels of urinary protein and hematuria. These observations suggested that the TX/PGI ratio was a useful index to assess the pathological condition of renal diseases and the effects of treatment.
Hishinuma
T
T
Department of Pharmaceutical Sciences, Tohoku University Hospital, Sendai, Japan.
Koseki
Y
Y
Katayama
J
J
Murai
Y
Y
Saito
T
T
Mizugaki
M
M
D000328:Adult; D016022:Case-Control Studies; D002908:Chronic Disease; D011464:Epoprostenol / Q000652:urine*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005921:Glomerulonephritis / Q000652:urine*; D006801:Humans; D007477:Ions; D008297:Male; D008875:Middle Aged; D013928:Thromboxane A2 / Q000652:urine*
D007477:Ions; D013928:Thromboxane A2; D011464:Epoprostenol