In vitro binding of nucleolin to double-stranded telomeric DNA.
We have purified a 100 kDa protein, resolved in a Southwestern binding screen of total nuclear proteins from Hela cells with double-stranded human telomeric probe. A polyclonal antiserum raised by this protein recognizes purified nucleolin and stains nucleoli in growing Hela cells. We demonstrate that a truncated form of human nucleolin and a purified deletion derivative of mouse nucleolin bind in vitro to duplex telomeric DNA. This study suggests a new link between telomeres and the nucleolus.
Pollice
A
A
Dipartimento di Genetica, Biologia Generale e Molecolare, Università degli Studi di Napoli Federico II, via Mezzocannone 8, Naples, 80134, Italy. pollice@biol.dgbm.unina.it
Zibella
M P
MP
Bilaud
T
T
Laroche
T
T
Pulitzer
J F
JF
Gilson
E
E
D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002466:Cell Nucleolus / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000378:metabolism*; D000097796:Nucleolin
D000906:Antibodies; D004247:DNA; D009687:Nuclear Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D000097796:Nucleolin