Recombinant human interleukin-11 has anti-inflammatory actions yet does not exacerbate systemic Listeria infection.
To determine whether recombinant human (rh) interleukin (IL)-11 disrupts the clearance of microbial pathogens, mice were challenged with Listeria monocytogenes after receiving high-dose rhIL-11, anti-tumor necrosis factor (TNF) monoclonal antibody (MAb), anti-IL-11 MAb, or saline control. The LD50 was not affected by rhIL-11 but was 10-fold lower in the anti-TNF MAb group (P<.001). Plasma IL-6, IL-1beta, and TNF-alpha levels were not different between rhIL-11-treated animals and the control group; however, interferon-gamma levels were significantly reduced by IL-11 treatment (2477 vs. 0 pg/mL, P<.01). Compared with the control group, the quantitative level of L. monocytogenes in hepatic and splenic tissue was unchanged by rhIL-11 but was significantly increased by TNF or IL-11 inhibition. The results indicate that IL-11 down-regulates cytokine production but does not exacerbate systemic infection in the murine Listeria infection model.
Opal
S M
SM
Division of Infectious Diseases, Brown University School of Medicine, Memorial Hospital of Rhode Island, Pawtucket, RI 02860, USA. Steven_Opal@brown.edu
Keith
J C
JC
Palardy
J E
JE
Parejo
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015169:Colony Count, Microbial; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D017370:Interleukin-11 / Q000276:immunology*; D008089:Listeria monocytogenes / Q000276:immunology* / Q000302:isolation & purification; D008088:Listeriosis / Q000276:immunology* / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000911:Antibodies, Monoclonal; D016207:Cytokines; D017370:Interleukin-11; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha