Determination of serum procarboxypeptidase A concentrations in healthy human adults.
Clostripain (EC 3.4.22.8) has been shown to be superior to trypsin as a means of activating serum procarboxypeptidase A. With this activation and a previously described assay for carboxypeptidase A it has been possible to determine the concentration of procarboxypeptidase A in human serum. In order to establish a baseline in the healthy adult a survey of the procarboxypeptidase A content of the serum of 66 blood donors was carried out. An average value of 9.7 microg/l was found for the proenzyme. This is in sharp contrast to a mean of 0.34 microg/l for the free enzyme present in serum that was not treated with clostripain [1].
Stewart
J D
JD
Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA.
Gilvarg
C
C
D000328:Adult; D000368:Aged; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods; D002268:Carboxypeptidases / Q000097:blood*; D043422:Carboxypeptidases A; D002417:Cattle; D003546:Cysteine Endopeptidases / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000097:blood*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D014357:Trypsin; D003546:Cysteine Endopeptidases; C019913:clostripain