Identification and characterization of functional subunits of Clostridium botulinum type A progenitor toxin involved in binding to intestinal microvilli and erythrocytes.
Clostridium botulinum type A hemagglutinin-positive progenitor toxin consists of three distinct components: neurotoxin (NTX), hemagglutinin (HA), and non-toxic non-HA (NTNH). The HA consists of four subcomponents designated HA1, 2, 3a and 3b. By employing purified toxin and GST-fusion proteins of each HA subcomponent, we found that the HA-positive progenitor toxin, GST-HA1 and GST-HA3b bind to human erythrocytes and microvilli of guinea pig upper small intestinal sections. The HA-positive progenitor toxin and GST-HA1 bind via galactose moieties, GST-HA3b binds via sialic acid moieties. GST-2 and GST-3a showed no detectable binding.
Fujinaga
Y
Y
Department of Bacteriology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama, Japan.
Inoue
K
K
Nomura
T
T
Sasaki
J
J
Marvaud
J C
JC
Popoff
M R
MR
Kozaki
S
S
Oguma
K
K
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A / Q000737:chemistry* / Q000378:metabolism; D003014:Clostridium botulinum; D004912:Erythrocytes / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006168:Guinea Pigs; D006388:Hemagglutinins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D006388:Hemagglutinins; C088300:NTNH protein, Clostridium botulinum; D011993:Recombinant Fusion Proteins; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; D019158:N-Acetylneuraminic Acid; D005690:Galactose