title: Nucleotide sequence of the gene encoding murine tissue factor pathway inhibitor-2.
abstract: Tissue factor pathway inhibitor-2 (TFPI-2), also known as placental protein 5, is a 32 kDa extracellular matrix-associated serine proteinase inhibitor consisting of three tandemly-arranged Kunitz-type domains. Two overlapping genomic clones containing sequences encoding murine TFPI-2 were isolated from a lambda FIXII 129 SVJ mouse genomic library, and the complete nucleotide sequence of the gene was determined. The murine TFPI-2 gene spans approximately 9.3 kilobases and consists of five exons and four introns. The nucleotide sequences surrounding all the exon-intron boundaries are highly conserved and obey the GT-AG rule. Each Kunitz-type domain is encoded by a single exon, similar to that observed for other Kunitz-type proteinase inhibitors. A total of 1,577 bp of the 3'-flanking region contains a probable polyadenylation site (ATTAAA) at +5,759 and an apparent cleavage or termination site (CATTG) at +6,170. The 5'-flanking region of the murine TFPI-2 gene contains a prototypical TATA box, a GC box and two CAAT boxes. In addition, several candidate transcription factor binding sites responsible for placenta-, endothelial cell-, and smooth muscle cell-specific expression of the TFPI-2 gene were also identified.
authors.affiliation: Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque 87131-5301, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Kazama
authors.forename: S
authors.initials: S
authors.lastname: Kamei
authors.forename: J L
authors.initials: JL
authors.lastname: Kuijper
authors.forename: D C
authors.initials: DC
authors.lastname: Foster
authors.forename: W
authors.initials: W
authors.lastname: Kisiel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016678:Genome; D006023:Glycoproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics
chemical_list: D006023:Glycoproteins; D011257:Pregnancy Proteins; D014157:Transcription Factors; C021240:tissue-factor-pathway inhibitor 2