title: The pilL and pilN genes of IncI1 plasmids R64 and ColIb-P9 encode outer membrane lipoproteins responsible for thin pilus biogenesis.
abstract: The predicted amino acid sequences of the pilL and pilN genes, required for the thin pilus formation of IncI1 plasmids R64 and ColIb-P9, contain N-terminal lipoprotein signal peptide motifs. The pilL and pilN products were labeled with [(3)H]palmitic acid as 38- and 57-kDa proteins, respectively, indicating that they are lipoproteins. Both PilL and PilN were localized to the outer membrane.
authors.affiliation: Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo, 192-0397, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Komano
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D018933:Pili, Sex / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins