Capillary electrophoresis: a new analytical tool for forensic toxicologists.
In capillary electrophoresis, electrophoretic or electrokinetic separations are carried out in tiny capillaries at high voltages (10-30 kV), thus achieving high efficiency (N > 105), resolution power, and mass sensitivity (down to 10(-18)-10(-20) moles). The main characteristics of capillary electrophoresis are versatility of application (from inorganic ions to large DNA fragments), use of different separation modes with different selectivity, low demands on sample volume, negligible running costs, possibility of interfacing with different detection systems including mass spectrometry, and the ruggedness and simplicity of the instrumentation. Capillary electrophoresis applications in the forensic sciences are now rapidly growing, particularly in forensic toxicology. The present paper briefly describes the basic principles of capillary electrophoresis and presents a selected review of its main applications to the analysis of illicit/controlled drugs in biologic samples. An original analytical approach to the determination of carbohydrate deficient transferrin, a new marker of chronic alcohol abuse, based on capillary electrophoresis is also described. It is concluded that the peculiar separation mechanisms and the high complementarity of capillary electrophoresis to chromatography make it a new powerful tool of investigation in the hands of forensic toxicologists.
Manetto
G
G
Department of Public Medicine and Health, University of Verona, Italy.
Crivellente
F
F
Tagliaro
F
F
D019075:Electrophoresis, Capillary; D005554:Forensic Medicine / Q000295:instrumentation*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis; D014116:Toxicology / Q000295:instrumentation*
D004364:Pharmaceutical Preparations