title: Influence of static magnetic field on the antiproliferative effects of vitamin D on human breast cancer cells.
abstract: We describe the effect of a 0.2 tesla (T) static magnetic field generated by a magnetic resonance tomograph and of vitamin D treatment on a human breast cancer cell line (MCF-7). Cell damage and proliferation were monitored by measuring the incorporation of [3H]thymidine in duplicating DNA and by the clonogenic assay. [3H]Thymidine incorporation in MCF-7 was stimulated by vitamin D at low doses (10(-12)-10(-10) M), whereas it was inhibited at higher concentrations (10(-9)-10(-6) M). Magnetic field treatment (0.2 T) decreased [3H]thymidine incorporation in human breast cancer cells, eliminating the proproliferative effect of low doses of vitamin D, and enhanced the vitamin D antiproliferative effect, further reducing [3H]thymidine incorporation, from -12.5% (P < 0.05) to -66.7% (P < 0.001), over the range of 10(-9) to 10(-6) M. In the clonogenic assay, ability of MCF-7 to form colonies was inhibited by vitamin D 10(-9) M and above, whereas 3-h exposure to 0.2 T magnetic field had no effect on the number of cell colonies formed. In conclusion, vitamin D treatment yields a permanent antiproliferative effect, while magnetic field exposure only temporarily slows down cellular growth. These findings suggest that therapy with vitamin D may prove beneficial for chemoprevention or treatment of breast cancer. Static magnetic field, alone or in combination, does not appear to represent an effective candidate for breast cancer therapy, at least at the intensity used in the present study.
authors.affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Firenze at the Careggi General Hospital, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Pacini
authors.forename: S
authors.initials: S
authors.lastname: Aterini
authors.forename: P
authors.initials: P
authors.lastname: Pacini
authors.forename: C
authors.initials: C
authors.lastname: Ruggiero
authors.forename: M
authors.initials: M
authors.lastname: Gulisano
authors.forename: M
authors.initials: M
authors.lastname: Ruggiero
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004574:Electromagnetic Fields; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure; D014410:Tumor Stem Cell Assay; D014807:Vitamin D / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D014807:Vitamin D