Two-enzyme system for the synthesis for 1-lauroyl-rac-glycerophosphate (lysophosphatidic acid) and 1-lauroyl-dihydroxyacetonephosphate.
A combination of two enzymes, phospholipase D (PL D) and C (PL C), was investigated for the production of two lysophospholipids, 1-lauroyl-rac-glycerophosphate (1-LGP) and 1-lauroyl-dihydroxyacetonephosphate (1-LDHAP). The high transphosphatidylation ability of phospholipase D from Streptomyces sp. allowed the formation of 1-lauroyl-phosphatidylglycerol (1-LPG) and 1-lauroyl-phosphatidyldihydroxyacetone (1-LPDHA) from phosphatidylcholine (PC) and 1-monolauroyl-rac-glycerol (1-MLG) and 1-lauroyl-dihydroxyacetone (1-MDHA), respectively. A two-phase system, diethyl ether/water, was chosen for the convenience of the recovery of the water insoluble products. A similar two-phase system was used for hydrolysis of the complex phospholipids by phospholipase C form Bacillus cereus, which released both lysophospholipids. Only trace amounts of phosphatidic acid (PA) were detected showing that the enzyme is highly selective for the release of the diacylglycerol and 1-lauroyl-rac-glycerophosphate and 1-lauroyl-dihydroxyacetonephosphate.
Virto
C
C
Department of Biotechnology, Centre for Chemistry and Chemical Engineering, Lund University, Sweden. carmen.virto@biotek.lu.se
Adlercreutz
P
P
D001409:Bacillus cereus / Q000201:enzymology; D007202:Indicators and Reagents; D007700:Kinetics; D008246:Lysophospholipids / Q000138:chemical synthesis*; D015394:Molecular Structure; D010739:Phospholipase D / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology; D010738:Type C Phospholipases / Q000378:metabolism
C404465:1-lauroyl-dihydroxyacetonephosphate; D007202:Indicators and Reagents; D008246:Lysophospholipids; D010738:Type C Phospholipases; D010739:Phospholipase D