[Extraction and purification of mouse H-2 antigen].
This study was designed to purify the mouse major histocompatibility complex antigen (H-2Ag). The detergent was used to extract the crude H-2Ag, and monoclonal antibodies affinity chromatography was applied to purify H-2 antigen. A 45 kd heavy chain and a 12 kd light chain from the purified proteins were shown by electrophoresis. Besides, the pure H-2 antigen was found to have immunological and biological activity. This method should be useful in purifying large quantities of H-2Ag for the researches in transplantation and functional analysis of H-2 antigen.
Lan
P
P
Department of Surgery, First Affiliated Hospital Chengdu.
Gu
X
X
Wang
J
J
D000818:Animals; D002846:Chromatography, Affinity; D003601:Cytotoxicity Tests, Immunologic; D006183:H-2 Antigens / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D051379:Mice
D006183:H-2 Antigens