title: Endothelial cell loss in irradiated optic nerves.
abstract: OBJECTIVE
  Radiation optic neuropathy usually occurs months to years after exposure of the anterior visual pathways to ionizing radiation. It is characterized by high signal on gadolinium-enhanced T1-weighted magnetic resonance imaging. Radiation-induced endothelial cell damage resulting in blood-nerve barrier breakdown is hypothesized to produce this pattern, but histologic evidence of this in the optic nerve is lacking. We attempted to evaluate the effect of radiation on endothelial cells in the optic nerve.
  
  
  METHODS
  Case-controlled histologic study.
  
  
  METHODS
  We studied the optic nerves of 16 enucleated eyes from patients with uveal melanoma treated with proton beam irradiation, 6 from normal eyes and 5 from eyes with unirradiated uveal melanomas. Binding of Ulex europaeus agglutinin I (UEA-I) lectin was used to identify endothelial cells in single paraffin sections. Transverse and longitudinal sections of vessels were counted in masked fashion.
  
  
  RESULTS
  There were 49.4+/-6.9 transversely sectioned endothelial cells per millimeter of nerve in 6 optic nerves exposed to 0 to 1000 cGyE ("low-dose") compared with 17.3+/-5.3 in 10 nerves exposed to 5500 to 7000 cGyE ("high-dose") (P = 0.002). Longitudinally sectioned vessels stained with UEA-I were separately identified, with 11.5+/-2.1 in the low-dose group and 5.6+/-1.6 in the high-dose group (P = 0.044). The thickness and staining of the endothelial cell layer appeared greater in the high-dose group. Endothelial cell counts did not correlate with age, gender, acuity, or interval after irradiation.
  
  
  CONCLUSIONS
  Increased radiation dosage to the optic nerve correlates with smaller numbers of endothelial cells.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison 53792, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Levin
authors.forename: E S
authors.initials: ES
authors.lastname: Gragoudas
authors.forename: S
authors.initials: S
authors.lastname: Lessell
mesh_terms: D000368:Aged; D002452:Cell Count; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism; D008545:Melanoma / Q000532:radiotherapy*; D008875:Middle Aged; D009900:Optic Nerve / Q000098:blood supply / Q000528:radiation effects*; D009901:Optic Nerve Diseases / Q000209:etiology* / Q000473:pathology; D037121:Plant Lectins; D011832:Radiation Injuries / Q000209:etiology* / Q000473:pathology; D011839:Radiation, Ionizing; D011879:Radiotherapy Dosage; D014604:Uveal Neoplasms / Q000532:radiotherapy*
chemical_list: D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins