title: The diagnosis of low-grade peripheral B-cell neoplasms in bone marrow trephines.
abstract: The aim of this study was to establish how effective is the use of immunohistochemistry on formalin-fixed bone marrow in diagnosing low-grade B-cell neoplasms. We investigated a series of 41 consecutive patients with bone marrow involvement for whom no other diagnostic tissues were available. The sections were stained with the following antibodies: CD3, CD20, CD79a, CD5, CD10, CD23, anti-cyclin D1 and kappa and lambda light chains. Antigen retrieval was performed using either a microwave oven or a pressure cooker. Labelling was performed with an avidin-biotin-peroxidase labelling system. A final diagnosis was reached in 37 out of 41 cases (90%): B-chronic lymphocytic leukaemia (15 cases), follicular lymphoma (10 cases), mantle-cell lymphoma (eight cases) and lymphoplasmacytoid lymphoma/immunocytoma (four cases). In the remaining four cases, a generic diagnosis of low-grade B-cell neoplasm was made. The immunophenotyping of formalin-fixed marrow is a useful technique for diagnosing most of the low-grade B-cell neoplasms.
authors.affiliation: Departments of Histopathology, University College London, University Street, London WC1E 6JJ, UK.
authors.forename: F
authors.initials: F
authors.lastname: Pezzella
authors.forename: P J
authors.initials: PJ
authors.lastname: Munson
authors.forename: K D
authors.initials: KD
authors.lastname: Miller
authors.forename: A H
authors.initials: AH
authors.lastname: Goldstone
authors.forename: K C
authors.initials: KC
authors.lastname: Gatter
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000175:diagnosis*; D008224:Lymphoma, Follicular / Q000175:diagnosis; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis
chemical_list: D015703:Antigens, CD