title: Determination of amino acid sequences of two subunits in sarcosine oxidase from Corynebacterium sp. U-96.
abstract: The primary structures of the C and D subunits of sarcosine oxidase from Corynebacterium sp. U-96 were determined by sequencing the peptide fragments derived from their enzymatic digestions. The C and D subunits were shown to be composed of 199 and 92 residues, respectively. Each amino acid sequence showed a high homology with the sequence of the corresponding subunit from Corynebacterium sp. P-1. However, there were some differences between these two species, that is, four N-terminal residues were truncated in the C subunit, but six C-terminal residues were truncated in the D subunit. The D subunit contained three cysteine residues, but no disulfide bonds are in the subunit. Overall sequences of both subunit showed no homology with any other protein in the data base.
authors.affiliation: Department of Biosciences, Faculty of Science, Kitasato University, Sagamihara, Kanagawa, Japan. mukoyama@jet.sci.kitasato-u.ac.jp
authors.forename: E B
authors.initials: EB
authors.lastname: Mukouyama
authors.forename: S
authors.initials: S
authors.lastname: Tatemukai
authors.forename: K
authors.initials: K
authors.lastname: Morioka
authors.forename: J
authors.initials: J
authors.lastname: Matsuura
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D003352:Corynebacterium / Q000201:enzymology*; D004220:Disulfides / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry* / Q000302:isolation & purification; D050879:Sarcosine Oxidase; D017386:Sequence Homology, Amino Acid
chemical_list: D004220:Disulfides; D010089:Oxidoreductases, N-Demethylating; D050879:Sarcosine Oxidase