Effect of irradiation on autogenous bone transplantation in rat parietal bone.
To determine the appropriate time for bone reconstruction after irradiation, the healing process after autogenous iliac bone transplantation in the irradiated parietal bone was examined by scanning electron microscopy and light microscopy. Bone transplantation was carried out at the second and the fourth weeks after Cobalt-sixty (60Co) irradiation with calculated dose and fractionation. Animals without irradiation were used as control. The results show the appearance of mesenchymal cells and blood vessels around the transplantation to be extremely few one week after transplantation which was carried out at the second week after irradiation. These inhibitions were still seen two weeks after transplantation. Four weeks after transplantation, there were no differences in the bone formation among the experimental groups. Bone formation in the transplantation at the fourth week after irradiation was similar to that of the control group. Microvascularization in the transplantation at the second week after irradiation was inhibited one week after transplantation. The delay in bone healing was responsible for the retardation of revascularization and caused microcirculatory failures as well as the damage of osteogenic cells. It is quite clear that damaged cells and tissues recovered by the elapse of time under the irradiation procedure employed in this study and also that bone formation was carried out in the physiological process. We think that bone transplantation after irradiation should be done after recovery from the radiation damage to the periosteal cells and the blood vessels.
Takekawa
M
M
Department of Oral and Maxillofacial Surgery, Asahikawa Medical College, Japan. mt906@asahikawa-med.ac.jp
Matsuda
M
M
Ohotubo
S
S
D000818:Animals; D016025:Bone Transplantation / Q000473:pathology / Q000502:physiology*; D001842:Bone and Bones / Q000098:blood supply / Q000528:radiation effects* / Q000648:ultrastructure; D002196:Capillaries / Q000502:physiology / Q000648:ultrastructure; D003037:Cobalt Radioisotopes; D007091:Image Processing, Computer-Assisted; D008297:Male; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic; D010521:Periosteum / Q000166:cytology / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014182:Transplantation, Autologous
D003037:Cobalt Radioisotopes