The pilL and pilN genes of IncI1 plasmids R64 and ColIb-P9 encode outer membrane lipoproteins responsible for thin pilus biogenesis.
The predicted amino acid sequences of the pilL and pilN genes, required for the thin pilus formation of IncI1 plasmids R64 and ColIb-P9, contain N-terminal lipoprotein signal peptide motifs. The pilL and pilN products were labeled with [(3)H]palmitic acid as 38- and 57-kDa proteins, respectively, indicating that they are lipoproteins. Both PilL and PilN were localized to the outer membrane.
Sakai
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Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo, 192-0397, Japan.
Komano
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D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D018933:Pili, Sex / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D013347:Subcellular Fractions / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins