Genetic organization and polymorphism of the guaA gene encoding the GMP synthetase in Lactobacillus rhamnosus.
The guaA gene encoding GMP synthetase was cloned from a potential probiotic strain of Lactobacillus rhamnosus. DNA sequence and Northern blot analysis indicated that (i) guaA did not belong to an guaAB operonic structure, conversely to enteric bacteria, (ii) L. rhamnosus guaA seemed to be highly expressed, and (iii) genetic regulation might differ from Bacillus subtilis. Moreover, differences in the genetic organization of guaA allowed discrimination of some closely related L. rhamnosus strains, with a rapid screening by PCR.
Grimaldi
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Institut de Génétique et Microbiologie, Centre National de Recherche Scientifique, Unité Mixte de Recherche 8621, Université Paris-Sud, Bâtiment 360, 91 405 Orsay Cedex, France.
Dutertre
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Simonet
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D019731:Carbon-Nitrogen Ligases / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
D004269:DNA, Bacterial; D019731:Carbon-Nitrogen Ligases; C020828:GMP synthase (glutamine-hydrolyzing)