E-Selectin expression in a murine model of chronic colitis.
The objective of this study was to quantify E-selectin surface expression in the colon as well as other tissues in a CD4(+) T-cell model of chronic colitis in mice using the newly developed dual radiolabel monoclonal antibody technique. Male SCID mice were reconstituted with either 5 x 10(5) CD4(+) CD45RB(low) or CD45RB(high) T-cells isolated from normal CB-17 donor mouse spleens and subsequently monitored for clinical signs of colitis. We found that animals injected with CD45RB(high) but not CD45RB(low) T-cells nor PBS developed colitis at 6-8 weeks following reconstitution as assessed by loss of body weight, development of loose stools and/or diarrhea, and histopathology. Concurrent with the onset of distal bowel inflammation was enhanced expression of E-selectin compared to SCID mice injected with PBS or reconstituted with CD45RB(low) T-cells, both of which did not develop colitis. We also observed significant increases in E-selectin expression in cecum, small intestine, mesentery, and liver of colitic mice. Our data confirm that reconstitution of SCID mice with CD45RB(high) but not CD45RB(low) T-cells induces chronic colitis and demonstrate that this chronic colitis is associated with enhanced expression of an endothelial cell-specific adhesion molecule. Furthermore, our studies demonstrate that reconstitution of SCID mice with CD45RB(high) T-cells enhances E-selectin expression in a variety of tissues distant from the site of active inflammation.
Kawachi
S
S
Department of Molecular and Cellular Physiology, LSU Health Sciences Center, Shreveport, Louisiana 71130, USA.
Morise
Z
Z
Conner
E
E
Laroux
F S
FS
Gray
L
L
Fuseler
J
J
Grisham
M B
MB
D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002908:Chronic Disease; D003092:Colitis / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D019040:E-Selectin / Q000096:biosynthesis*; D004727:Endothelium / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D016513:Mice, SCID; D011863:Radioimmunoassay / Q000379:methods
D015815:Cell Adhesion Molecules; D019040:E-Selectin