title: Calcyclin is differentially expressed in rat testicular cells.
abstract: Immature rat Sertoli cells aggregate and form tubule-like structures when cultured on a monolayer of peritubular myoid cells. In this study, differential gene expression of monocultures and direct cocultures of peritubular cells and Sertoli cells were examined. One of the cDNA clones isolated showed high homology to calcyclin and a microvascular differentiation gene, CEC5, which was reported to be highly homologous to CASK, a membrane-associated guanylate kinase homolog. Sequencing and mRNA analysis of rat calcyclin demonstrated that the gene was differentially expressed and was found only in peritubular cells and cocultures with increased levels. In contrast, CASK was expressed by Sertoli cells, peritubular cells, and cocultures, whereas CEC5 was never found in the testicular somatic cells. Our findings point to a paracrine regulation of calcyclin expression in testicular peritubular fibroblasts which seems to be related to tubular growth.
authors.affiliation: Department of Anatomy and Cell Biology, Philipps University, Marburg, Germany. konrad@mailer.uni-marburg.de
authors.forename: L
authors.initials: L
authors.lastname: Konrad
authors.forename: G
authors.initials: G
authors.lastname: Aumüller
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002478:Cells, Cultured; D018920:Coculture Techniques; D004247:DNA; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012708:Sertoli Cells / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489132:S100a6 protein, rat; C055351:S100A6 protein, human; D004247:DNA