title: The crystal proteins from Bacillus thuringiensis subsp. thompsoni display a synergistic activity against the codling moth, Cydia pomonella.
abstract: Crystal proteins from Bacillus thuringiensis subsp. thompsoni strain HnC are active against the codling moth, Cydia pomonella, a major pest of orchards. Inclusion bodies purified from strain HnC displayed an LC(50) of 3.34 x 10(-3) microgram/microliter. HnC-purified crystals were tenfold more active than Cry2Aa and Cry1Aa toxins, and 100-fold more toxic than Cry1Ab. The 34-kDa and 40-kDa proteins contained in HnC inclusion bodies were shown to act synergistically. The toxicity of crystal proteins produced by the recombinant B. thuringiensis strain BT-OP expressing the full-length native operon was about tenfold higher than that of the 34-kDa protein. When the gene encoding the non-insecticidal 40-kDa protein, which is not active, was introduced into the recombinant strain producing only the 34-kDa protein, the toxicity was raised tenfold and was similar to that of the strain BT-OP.
authors.affiliation: CIRAD, Avenue Agropolis, B.P. 5035, 34032 Montpellier Cedex 1, France.
authors.forename: C
authors.initials: C
authors.lastname: Rang
authors.forename: L A
authors.initials: LA
authors.lastname: Lacey
authors.forename: R
authors.initials: R
authors.lastname: Frutos
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000737:chemistry; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000633:toxicity*; D001427:Bacterial Toxins; D001681:Biological Assay / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004731:Endotoxins / Q000633:toxicity*; D006460:Hemolysin Proteins; D002479:Inclusion Bodies / Q000737:chemistry; D007306:Insecticides / Q000633:toxicity*; D007814:Larva / Q000187:drug effects; D009036:Moths / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis