Vascular biosynthesis of nitric oxide: effect on hemostasis and fibrinolysis.
Nitric oxide (NO) is a multifunctional effector molecule that plays a central role in the regulation of vascular homeostasis. NO is synthesized from L-arginine by a family of enzymes called NO synthases. The principal source of NO in the vascular system of healthy mammals is the constitutively expressed NO synthase in endothelial cells. The basal endothelial formation of NO can be increased by receptor-dependent agonists (i.e., bradykinin) in a calcium-calmodulin-dependent manner, and also by physical forces (i.e., shear stress), predominantly without changes in the intracellular concentration of free calcium. Nitric oxide can diffuse toward the blood vessel wall where the major target is the smooth muscle cell. NO regulates vascular tone, and the free radical is also a potent inhibitor of smooth muscle cell proliferation, migration and synthesis of extracellular matrix proteins. NO can also diffuse toward the lumen of the blood vessel where it helps maintain blood fluidity. NO inhibits platelets' and leucocytes' adhesion to endothelial cells. In addition, NO inhibits platelet aggregation and facilitates the dissolution of small platelet aggregates. However, the regulatory action of NO on blood cells is most likely limited to the luminal surface of endothelial cells since NO is rapidly scavenged by hemoglobin in erythrocytes and inactivated by oxygen-derived radicals such as superoxide anions. NO can also affect the fibrinolytic activity by regulating the release of tissue-type plasminogen activator and plasminogen activator inhibitor-1. The crucial role of vascular NO in the control of blood fluidity has been demonstrated by the regulation of the bleeding time in humans.
Schini-Kerth
V B
VB
Institut für Kardiovaskuläre Physiologie, Klinikum der JWG-Universität, Frankfurt am Main, Germany.
D000818:Animals; D001120:Arginine / Q000378:metabolism; D020013:Calcium Signaling; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000378:metabolism*; D005342:Fibrinolysis / Q000502:physiology*; D016166:Free Radical Scavengers / Q000378:metabolism; D005609:Free Radicals; D006454:Hemoglobins / Q000378:metabolism; D006487:Hemostasis / Q000502:physiology*; D006801:Humans; D008322:Mammals / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D010973:Platelet Adhesiveness / Q000502:physiology; D013481:Superoxides / Q000378:metabolism; D014661:Vasoconstriction / Q000502:physiology
D016166:Free Radical Scavengers; D005609:Free Radicals; D006454:Hemoglobins; D013481:Superoxides; D009569:Nitric Oxide; D001120:Arginine; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III