Interactions between food components and drugs. Part 8: Effect of pectins and bile acid preparations forming stable mixed micelles on transport of quinine in vitro.
Interactions between quinine and acetylated pectin, amidated pectin and pectin with blockwise arrangement of the free carboxyl groups as well as interactions between quinine and bile salt preparations forming stable mixed bicelles have been investigated. A diffusion cell with two compartments and an artificial lipid membrane and a filter-grown colon carcinoma cell line (Caco-2) have been used. Depending on structural parameters, pectin preparations diminished the rate of permeation of the drug. Above the critical micelle concentration, the bile salt preparations influence the quinine transport stronger than the pectin preparations. The strongest inhibition of the quinine permeation showed a stable mixed micelle preparation consisting of glycodeoxycholate, palmitic acid and lecithin. The Caco-2 cell line appears to be not as suitable as artificial lipid membranes to study drug transport in the presence of the bile salt preparations.
Fritzsch
B
B
Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany.
Neubert
R H
RH
Dongowski
G
G
Heinevetter
L
L
D000962:Antimalarials / Q000737:chemistry / Q000493:pharmacokinetics*; D001647:Bile Acids and Salts / Q000737:chemistry*; D018938:Caco-2 Cells; D005079:Excipients; D018565:Food-Drug Interactions; D006801:Humans; D008823:Micelles; D010368:Pectins / Q000737:chemistry*; D010539:Permeability; D011803:Quinine / Q000737:chemistry / Q000493:pharmacokinetics*
D000962:Antimalarials; D001647:Bile Acids and Salts; D005079:Excipients; D008823:Micelles; D010368:Pectins; D011803:Quinine