title: Effect of electron microscopy fixation pH on the ultrastructure of soybean protein bodies.
abstract: Cotyledon tissues from mature soybeans were systematically prepared for transmission electron microscopy employing fixation buffer pH's of 7.2, 6.4, and 5.6. Tissue fixed at pH 7.2 showed few membrane-bound internal protein body structures. Portions of the same tissue fixed at pH 6.4 revealed numerous membrane-bound crystalloid structures and stacks of membranous sacks. Tissue prepared at pH 5.6 also contained numerous membrane-bound crystalloid structures and examples of membrane-bound globoid structures. This is the first investigation to document the effect of fixation pH on the subcellular structure of soybean protein bodies and the first to show membrane-bound crystalloid or globoid structures in soybeans.
authors.affiliation: Animal Science Department, University of Kentucky, Lexington, Kentucky 40546-0215, and Department of Plant Pathology, University of Arkansas, Fayetteville, Arkansas 72704, USA. wlboat1@pop.uky.edu
authors.forename: W L
authors.initials: WL
authors.lastname: Boatright
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
mesh_terms: D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D010940:Plant Proteins / Q000648:ultrastructure*; D013025:Glycine max / Q000648:ultrastructure*
chemical_list: D010940:Plant Proteins