title: Human B-cell lines constitutively express and secrete interleukin-16.
abstract: Interleukin-16 (IL-16), produced by activated CD8+ T lymphocytes, is inhibitory to human immunodeficiency virus-1 (HIV-1) replication. In an attempt to determine whether human B cells express and secrete IL-16, a wide panel of B-cell lines derived from patients with acquired immune deficiency syndrome (AIDS)-associated B-cell lymphomas (AABCL) (n = 5) and from non-AABCLs (n = 8) were studied. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we were able to observe ubiquitous expression of IL-16 mRNA. Kinetic studies on constitutive mRNA turnover and secretion for IL-16 suggests that the optimum expression is at 24 hr. Interestingly, we report, for the first time, IL-16 secretion by human B-cell lines.
authors.affiliation: Laboratory of Cytokine Research, Department of Biology, University of West Florida, Pensacola, FL 32514, USA.
authors.forename: V
authors.initials: V
authors.lastname: Sharma
authors.forename: J L
authors.initials: JL
authors.lastname: Sparks
authors.forename: J D
authors.initials: JD
authors.lastname: Vail
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D015870:Gene Expression; D006801:Humans; D019410:Interleukin-16 / Q000235:genetics / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D019410:Interleukin-16; D012333:RNA, Messenger; D012334:RNA, Neoplasm