Sequence analysis of a total of three megabases of DNA in two regions of chromosome 8p.
Large-scale sequencing of genomic regions and in silico gene trapping together represent a highly efficient and powerful approach for identifying novel genes. We performed megabase-level sequence analyses of two genomic regions on human chromosome 8p (8p11.2 and 8p22-->p21.3), after covering those segments with sequence-ready contigs composed of 74 cosmids, 14 BACs, and three PAC clones. We determined continuous nucleotide sequences of 1,856,753 bases on 8p11.2 and 1,210,381 bases on 8p22-->p21.3 by combining the shotgun and primer-walking methods. In silico gene trapping identified four novel genes in the 8p11.2 region and, in the 8p22-->p21.3 region, six known genes (PRLTS, PCM1, MTAMR7, HCAT2, HFREP-1 and PHP) and three novel genes. The distribution of Alu and LINE1 repetitive elements and the densities of predicted exons were different in each region, and Alu-rich portions contained more exonic sequences than LINE1-rich areas.
Isomura
M
M
Department of Human Genome Analysis, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo.
Ikegawa
S
S
Kinjo
T
T
Takeuchi
K
K
Yamane-Tanaka
Y
Y
Kitami
K
K
Nakamura
Y
Y
D000595:Amino Acid Sequence; D002898:Chromosomes, Human, Pair 8; D020451:Contig Mapping; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA