title: Direct quantitative transcript analysis of the agr regulon of Staphylococcus aureus during human infection in comparison to the expression profile in vitro.
abstract: Bacteria possess a repertoire of distinct regulatory systems promoting survival in disparate environments. Under in vitro conditions it was demonstrated for the human pathogen Staphylococcus aureus that the expression of most virulence factors is coordinated by the global regulator agr. To monitor bacterial gene regulation in the host, we developed a method for direct transcript analysis from clinical specimens. Quantification of specific transcripts was performed by competitive reverse transcription-PCR, and results were normalized against the constitutively expressed gene for gyrase (gyr). Using sputum from cystic fibrosis (CF) patients infected with S. aureus we examined the transcription of the effector molecule RNAIII of agr, of spa (protein A), generally repressed by agr, and of hla (alpha-toxin), generally activated by agr. In the CF lung RNAIII was expressed poorly, indicating an inactive agr in vivo. Despite the low level of RNAIII expression, spa was detectable only in minute amounts and an irregular transcription of hla was observed in all sputum samples. After subculturing of patient strains agr-deficient isolates and isolates with unusual expression profiles, i.e., not consistent with those obtained from prototypic strains, were observed. In conclusion, the agr activity seems to be nonessential in CF, and from the described expression pattern of spa and hla, other regulatory circuits aside from agr are postulated in vivo.
authors.affiliation: Allgemeine Hygiene und Umwelthygiene, Universität Tübingen, Tübingen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Goerke
authors.forename: S
authors.initials: S
authors.lastname: Campana
authors.forename: M G
authors.initials: MG
authors.lastname: Bayer
authors.forename: G
authors.initials: G
authors.lastname: Döring
authors.forename: K
authors.initials: K
authors.lastname: Botzenhart
authors.forename: C
authors.initials: C
authors.lastname: Wolz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000150:complications; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D008171:Lung Diseases / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D018085:Regulon; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013183:Sputum / Q000382:microbiology; D013203:Staphylococcal Infections / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D012333:RNA, Messenger; D013205:Staphylococcal Protein A; D015534:Trans-Activators; D014157:Transcription Factors; C021091:staphylococcal alpha-toxin