title: [Intraepithelial enteroendocrine cells in cecum and appendix from ovoalbumin sensitized rabbits].
abstract: Intraepithelial enteroendocrine cells (IEC) produce peptides which influence motility, secretion and absorption of nutrients. Recently the role of these cells in the immune mucosal system is under study. The aim of the present study was to evaluate the modifications in number of IEC in cecum and appendix from Ovalbumin (OVA) sensitized rabbits. Twenty adult New Zealand rabbits were separated in two groups: Group 1 (G1 = 10) not sensitized normal control. Group 2 (G2 = 10) were sensitized twice intraperitoneally with 70 mg OVA and 30 mg ALUM/ml (aluminium hydroxide). Anti OVA specific IgE was evaluated by means of PCA test (passive cutaneous anaphylaxis). Samples form cecum and appendix were fixed in buffered formaldehyde 10%, paraffin embedded and stained with anti-Chromogranin A for neuroendocrine cells. 400 high power fields were counted in each animal, referred as IEC/100 enterocytes. In cecum surface epithelium and crypt were considered. Surface epithelium, deep crypts and superficial crypts were evaluated in appendix. Results showed in cecum in G1:1,6 IEC/100 enterocytes in surface epithelium and 3/100 in crypts; G2 6 IEC/100 in surface epithelium and 12/100 in crypts. The difference between G1 and G2 was statistically significant (p < 0.05). In appendix surface epithelium from G1 showed 5.2/100 while G2 5.4/100. Superficial crypts 8.5 (G1) and 11.3 (G2) (p < 0.05) and deep crypts 4.9 (G1) and 8.5 (G2) (p < 0.01). The results showed that OVA-sensitized animals presented increment in the number of IEC in surface epithelium and crypts which may indicate a relationship between these cells and rabbit mucosal immune system.
authors.affiliation: Cátedra de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Casilda, Argentina.
authors.forename: N
authors.initials: N
authors.lastname: Bassan
authors.forename: M
authors.initials: M
authors.lastname: Vinuesa
authors.forename: F
authors.initials: F
authors.lastname: Pérez
authors.forename: S
authors.initials: S
authors.lastname: Roma
authors.forename: S
authors.initials: S
authors.lastname: Bernardi
mesh_terms: D000818:Animals; D001065:Appendix / Q000166:cytology; D002432:Cecum / Q000166:cytology*; D002452:Cell Count; D019858:Enteroendocrine Cells / Q000166:cytology* / Q000276:immunology; D007114:Immunization; D007413:Intestinal Mucosa / Q000166:cytology*; D010047:Ovalbumin / Q000276:immunology; D011817:Rabbits
chemical_list: D010047:Ovalbumin