title: Virus-induced permeability transition in mitochondria.
abstract: Isolated rat liver mitochondria undergo permeability transition after supplementation with a suspension of tobacco mosaic virus. Four mitochondrial parameters proved the opening of the permeability transition pore in the inner mitochondrial membrane: increased oxygen consumption, collapse of the membrane potential, release of calcium ions from mitochondria, and high amplitude mitochondrial swelling. All virus-induced changes in mitochondria were prevented by cyclosporin A. These effects were not observed if the virus was treated with EGTA or disrupted by heating. Protein component of the virus particle in the form of 20S aggregate A-protein, or helical polymer, as well as supernatant of the heat-disrupted virus sample, had no effect on mitochondrial functioning. Electron microscopy revealed the direct interaction of the virus particles with isolated mitochondria. The possible role of the mitochondrial permeability transition pore in virus-induced apoptosis is discussed.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
authors.forename: L D
authors.initials: LD
authors.lastname: Zorova
authors.forename: B F
authors.initials: BF
authors.lastname: Krasnikov
authors.forename: A E
authors.initials: AE
authors.lastname: Kuzminova
authors.forename: I A
authors.initials: IA
authors.lastname: Polyakova
authors.forename: E N
authors.initials: EN
authors.lastname: Dobrov
authors.forename: D B
authors.initials: DB
authors.lastname: Zorov
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology*; D012367:RNA, Viral / Q000378:metabolism; D051381:Rats; D014027:Tobacco Mosaic Virus / Q000502:physiology*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral