[Study on a deletion polymorphism of the angiotensin converting enzyme gene in pregnancy induced hypertension].
OBJECTIVE
To study a polymorphism and allele frequency of the angiotensin converting enzyme (ACE) gene in pregnancy induced hypertension (PIH).


METHODS
Polymerase chain reaction (PCR) for detection of ACE gene polymorphism was performed to show the deletion/insertion (D/I) polymorphism in intron 16 of ACE gene. A 490 bp(I) and 190 bp(D) PCR products were identified, corresponding to the PCR amplification of the the allele with or without the insertion.


RESULTS
Derived allele frequencies for insertion and deletion were the different between 35 PIH and 25 control subjects. Compared the frequency of D allele gene (0.76) and the percentage of the ACE DD genotype (65.7%) in the PIH patients with the frequency (0.28) and the percentage (8.0%) in the control population, they were significant higher in individuals with PIH (P < 0.001).


CONCLUSIONS
There was an excess of DD genotype and the frequency of D allele gene in PIH, confirming the genetic variation in the ACE locus could be involved in the risk of PIH and suggesting the ACE gene may contribute to the pathogenesis of PIH.
Zhu
M
M
Hospital of Obstetrics & Gynecology, Shanghai Medical University.
Xia
Y
Y
Cheng
W
W
D000328:Adult; D005260:Female; D017353:Gene Deletion; D006801:Humans; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000201:enzymology / Q000235:genetics*; D011247:Pregnancy
D007703:Peptidyl-Dipeptidase A