Complex duplications at 6p22.1, 6p11.2, 5q13, 5p15.1 and 5p13 revealed by fluorescent in situ hybridisation.
A complex pattern of fluorescent in situ hybridisation (FISH) has been detected using PAC clones from the short arm of chromosome 6, proximal to the haemochromatosis gene at 6p22.1. Cross-hybridisation to 6p22.1, 6p11.2, 5q13, 5p15.1 and 5p13 was consistently detected with several PAC clones covering a genomic region greater than 200 kb. These results indicate that large sections of genomic DNA are shared by these 5 disparate chromosomal segments, indicative of large scale duplication events. These results were in part accounted for by the identification of several expressed sequence tags (ESTs).
Rhodes
D A
DA
Department of Human Immunogenetics, Imperial Cancer Research Fund, London, UK.
Volz
A
A
Jones
T
T
Ziegler
A
A
Trowsdale
J
J
D002895:Chromosomes, Human, Pair 5 / Q000235:genetics*; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D020224:Expressed Sequence Tags; D020440:Gene Duplication; D006432:Hemochromatosis / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence