Decreased apolipoprotein C-III concentration in the high-density lipoprotein fraction from calves inoculated with Pasteurella haemolytica and bovine herpes virus-1.
Lipoprotein lipid and apoprotein concentrations are known to be altered during the acute-phase response. We have previously shown that the serum activity of lecithin:cholesterol acyltransferase (LCAT) and concentration of cholesteryl esters, both constituents of the high-density lipoprotein (HDL) fraction, are reduced in calves inoculated with Pasteurella haemolytica and bovine herpes virus-1, the two major pathogens for calf pneumonia. The concentration of apolipoprotein C-III (apoC-III), a low molecular mass protein component distributed mainly in the HDL fraction, was therefore examined in bacteria- and virus-inoculated calves. An enzyme-linked immunosorbent assay demonstrated that it was decreased by inoculations of Pasteurella haemolytica and bovine herpes virus-1. The decrease was detected as early as 1 day after inoculation in both groups. A decreased serum apoC-III concentration was also observed by immunoblot analysis. It was detected in the HDL fractions from the bacteria- and virus-inoculated calves, and HDL apoC-III concentrations in the inoculated calves were decreased compared with controls. These results, coupled with the previous findings on LCAT activity and the cholesteryl ester concentration, indicate that a decreased HDL concentration is one of the early events occurring during the acute-phase response evoked by infections with Pasteurella haemolytica and bovine herpes virus-1.
Yamamoto
M
M
Fukushima Kenchu Livestock Hygiene Service Center, Kooriyama, Japan.
Katoh
N
N
D000818:Animals; D001056:Apolipoproteins C / Q000097:blood*; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000382:microbiology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006566:Herpesviridae Infections / Q000097:blood / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine; D008075:Lipoproteins, HDL / Q000737:chemistry; D008168:Lung / Q000382:microbiology / Q000821:virology; D008297:Male; D016978:Mannheimia haemolytica; D012766:Pasteurellosis, Pneumonic / Q000097:blood*; D018410:Pneumonia, Bacterial / Q000209:etiology / Q000662:veterinary; D011024:Pneumonia, Viral / Q000209:etiology / Q000662:veterinary
D001056:Apolipoproteins C; D008075:Lipoproteins, HDL