The angiogenic factor interleukin 8 is induced in non-small cell lung cancer/pulmonary fibroblast cocultures.
The interactions between tumor cells and surrounding stromal elements may promote the release of angiogenic factors. Although interleukin 8 (IL-8) is a major angiogenic factor in non-small cell lung cancer (NSCLC), the stromal contribution to IL-8 expression in primary NSCLC remains to be defined. To elucidate the role of stromal elements in NSCLC IL-8 production, normal pulmonary fibroblasts were cocultured with six representative NSCLC lines in direct and transwell assays. IL-8 transcripts and protein were consistently induced in fibroblasts and a subset of NSCLCs as a consequence of tumor/stromal coculture. In these cocultures, IL-8 was induced by IL-1alpha and an additional, as yet unidentified, soluble factor. These data underscore the importance of tumor/stromal interaction in the production of angiogenic peptides such as IL-8 in NSCLC.
Anderson
I C
IC
Department of Adult Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
Mari
S E
SE
Broderick
R J
RJ
Mari
B P
BP
Shipp
M A
MA
D000328:Adult; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology / Q000503:physiopathology*; D002450:Cell Communication; D002460:Cell Line; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics*; D008168:Lung / Q000166:cytology / Q000502:physiology*; D008175:Lung Neoplasms / Q000473:pathology / Q000503:physiopathology*; D017154:Stromal Cells / Q000166:cytology / Q000502:physiology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D016209:Interleukin-8