Transcription factor Sp3 is essential for post-natal survival and late tooth development.
Sp3 is a ubiquitously expressed transcription factor closely related to Sp1 (specificity protein 1). We have disrupted the mouse Sp3 gene by homologous recombination. Sp3-deficient embryos are growth retarded and invariably die at birth of respiratory failure. The cause for the observed breathing defect remains obscure since only minor morphological alterations were observed in the lung, and surfactant protein expression is indistinguishable from that in wild-type mice. Histological examinations of individual organs in Sp3(-/-) mice show a pronounced defect in late tooth formation. In Sp3 null mice, the dentin/enamel layer of the developing teeth is impaired due to the lack of ameloblast-specific gene products. Comparison of the Sp1 and Sp3 knockout phenotype shows that Sp1 and Sp3 have distinct functions in vivo, but also suggests a degree of functional redundancy.
Bouwman
P
P
Institut für Molekularbiologie und Tumorforschung, Emil-Mannkopff-Strasse 2.
Göllner
H
H
Elsässer
H P
HP
Eckhoff
G
G
Karis
A
A
Grosveld
F
F
Philipsen
S
S
Suske
G
G
D000565:Ameloblasts / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D005260:Female; D015870:Gene Expression; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009805:Odontogenesis / Q000235:genetics* / Q000502:physiology*; D010641:Phenotype; D011247:Pregnancy; D012131:Respiratory Insufficiency / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016329:Sp1 Transcription Factor / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D051705:Sp3 Transcription Factor; D014070:Tooth / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology
D017931:DNA Primers; D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; C497122:Sp3 protein, mouse; D014157:Transcription Factors; D051705:Sp3 Transcription Factor