Regression of experimental liver tumor after distant intra-hepatic injection of cytosine deaminase-expressing tumor cells and 5-fluorocytosine treatment.
Cytosine deaminase (CD) gene of E. coli converts the non-toxic compound 5-fluorocytosine (5-FC) into 5-fluorouracil. We have introduced a vector expressing the CD gene in a rat colon carcinoma cell line. Expression of the CD gene confers 5-FC sensitivity to these cells in vitro and in vivo. In a bifocal model consisting in a simultaneous engrafment of a CD+ tumor on one lobe of the liver and a wild-type parental tumor on the opposite lobe, treatment with 5-FC results in regression of both type of tumors, indicating the existence of a distant bystander effect.
Pierrefite-Carle
V
V
Unite INSERM 364, Faculte de Medecine, 06107 Nice, Cedex 2, France.
Baque
P
P
Gavelli
A
A
Benchimol
D
D
Bourgeon
A
A
Milano
G
G
Saint-Paul
M C
MC
Rossi
B
B
D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology; D043525:Cytosine Deaminase; D005437:Flucytosine / Q000494:pharmacology*; D015316:Genetic Therapy; D007267:Injections; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D008297:Male; D009368:Neoplasm Transplantation; D009700:Nucleoside Deaminases / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured
D005437:Flucytosine; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase