title: Determination of phenobarbital in plasma by micellar liquid chromatography.
abstract: A new liquid chromatographic procedure for the determination of phenobarbital in plasma samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C(18) analytical column, a guard column of similar characteristics, and a 0.03 M CTAB-3% 1-propanol at pH 7 mobile phase. The UV detector was set at 250 nm. Butabarbital was used as internal standard. Sample preparation only required the addition to the plasma samples of a 0.1 M SDS solution at pH 3 and centrifugation before injection into the chromatographic system. The limit of detection was 0.83 microg/mL of phenobarbital in plasma samples. The coefficients of variation were lower than 7. 5%.
authors.affiliation: Departamento de Química Analítica, Facultad de Farmacia, Universitat de València, C/ Vicente A. Estellés s/n, E-46100 Burjassot, València, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Martín-Biosca
authors.forename: S
authors.initials: S
authors.lastname: Sagrado
authors.forename: R M
authors.initials: RM
authors.lastname: Villanueva-Camãnas
authors.forename: M J
authors.initials: MJ
authors.lastname: Medina-Hernández
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008823:Micelles; D010634:Phenobarbital / Q000097:blood*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008823:Micelles; D010634:Phenobarbital