title: The structure and function of gramicidin A embedded in interdigitated bilayer.
abstract: The effects of phase transition from normal to interdigitated lipid bilayer on the function and structure of membrane proteins were studied using linear gramicidin (gramicidin A) as a model. Interdigitated bilayer structure of dipalmitoylphosphatidylglycerol (DPPG) liposomes that was induced by atropine could not be changed notably by intercalating of gramicidin. The K+ transportation of gramicidin in both normal and interdigitated bilayer was assayed by measuring the membrane potential. Results showed that gramicidin in interdigitated bilayer exhibited lower transport capability. Intrinsic fluorescence spectrum of gramicidin in interdigitated bilayer blue-shifted 2.8 nm from the spectrum in normal bilayer, which means that interdigitation provides a more hydrophobic environment for gramicidin. Circular dichroism measurement results indicated that the conformation of gramicidin in interdigitated bilayer is not the typical beta6.3 helix as in the normal bilayer. The results suggested that the interdigitated lipid bilayer might largely affect the structure and function of membrane proteins.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, People's Republic of China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Hao
authors.forename: G J
authors.initials: GJ
authors.lastname: Zhang
authors.forename: J W
authors.initials: JW
authors.lastname: Chen
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D052160:Benzothiazoles; D002232:Carbocyanines; D002942:Circular Dichroism; D005456:Fluorescent Dyes; D006096:Gramicidin / Q000737:chemistry*; D007364:Intercalating Agents; D007700:Kinetics; D008051:Lipid Bilayers / Q000737:chemistry*; D008564:Membrane Potentials; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D008051:Lipid Bilayers; D006096:Gramicidin; D015060:1,2-Dipalmitoylphosphatidylcholine; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine