Chromatographic approach to study beta-cyclodextrin as a promoter of the penetration of bifonazole into keratinic tissue.
A high-performance liquid chromatographic method for the determination of bifonazole in dimethyl sulfoxide solvent was developed to study its penetration into sheephoof. The analytical method was linear over the concentration range studied, i.e., from 0.1 mg/ml to 1 mg/ml. The relative standard deviation was less than 2%. The data obtained showed that complex forming with beta-cyclodextrin greatly improved the penetration of bifonazole.
Guillaume
Y C
YC
Laboratoire de Chimie Analytique, Faculté de Medecine Pharmacie, Besanfon, France.
Peyrin
E
E
Millet
J
J
D000818:Animals; D000935:Antifungal Agents / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000493:pharmacokinetics*; D006724:Hoof and Claw / Q000378:metabolism*; D007093:Imidazoles / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012756:Sheep; D047392:beta-Cyclodextrins
D000935:Antifungal Agents; D003505:Cyclodextrins; D007093:Imidazoles; D047392:beta-Cyclodextrins; C031215:betadex; C036596:bifonazole