title: Crystallization and preliminary X-ray diffraction studies of human epidermal growth factor.
abstract: Human epidermal growth factor (hEGF), a 6.2 kDa protein of 53 amino acids with three internal disulfide bridges, has been crystallized by the hanging-drop method. hEGF crystallizes in space group P3(1)21 (or P3(2)21) using MgCl(2) as precipitant, with unit-cell parameters a = b = 61.4, c = 87.0 A. Another type of crystal, obtained using NaCl as precipitant, belongs to a tetragonal point group and has unit-cell dimensions a = b = 102.5, c = 166.6 A. The trigonal crystals with the smaller unit cell diffract X-rays better and a native data set from a single crystal has been collected to 3.0 A resolution.
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Chai
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: B R
authors.initials: BR
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Luo
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: R C
authors.initials: RC
authors.lastname: Bi
authors.forename: C H
authors.initials: CH
authors.lastname: He
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D004815:Epidermal Growth Factor