The Bacillus subtilis ctaB paralogue, yjdK, can complement the heme A synthesis deficiency of a CtaB-deficient mutant.
Heme A is a prosthetic group in many respiratory oxidases. It is synthesised from heme B (protoheme IX) with heme O as an intermediate. In Bacillus subtilis two genes required for heme A synthesis, ctaA and ctaB, have been identified. CtaB is the heme O synthase and CtaA is involved in the conversion of heme O to heme A. A ctaB paralogue, yjdK, has been identified through the B. subtilis genome sequencing project. In this study we show that when carried on a low copy number plasmid, the yjdK gene can complement a ctaB deletion mutant with respect to heme A synthesis. Our results indicate that YjdK has heme O synthase activity. We therefore suggest that yjdK be renamed as ctaO.
Throne-Holst
M
M
Department of Microbiology, Lund University, Sölvegatan 12, S-22362, Lund, Sweden. mimmi.throne_holst@mikrbiol.lu.se
Hederstedt
L
L
D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D003062:Codon; D006418:Heme / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008969:Molecular Sequence Data
D001426:Bacterial Proteins; D003062:Codon; C027728:heme a; D006418:Heme; D019883:Alkyl and Aryl Transferases; C078356:CyoE protein, Bacteria