Relationship between disease duration and predominant orbital T cell subset in Graves' ophthalmopathy.
We sought to determine whether the predominant orbital T helper (T(H)) cell subset in orbital T cell clones established from patients with Graves' ophthalmopathy (GO) might be related to disease duration. A total of 117 clones were established from orbital adipose/connective tissues of 6 GO patients, and cytokine production was measured in 57 CD3+CD4+ clones. T(H)1-type clones were predominant in cultures from patients with recent onset (<2 yr) Graves' hyperthyroidism (n = 44; TH1/TH0/TH2 = 57/29/14%) or GO (n = 53 clones; TH1/TH0/TH2 = 47/30/23%). In contrast, TH2-type clones predominated in cultures from patients with more remote onset (>2 yr) hyperthyroidism (n = 13; TH1/TH0/TH2 = 0/31/69%; P < 0.005) or GO (n = 4; TH1/TH0/TH2 = 0/25/75%; P = 0.05). In addition, we established T cell clones from 1 TH1-dominant patient with recent-onset thyroid and eye disease using either IL-2 (12.5 ng/mL) alone or IL-2 plus IL-4 (5 ng/mL) and found no shift toward recovery of TH2-type clones in the latter. In conclusion, although the CD3+CD4+ clones characterized were not necessarily tissue antigen specific, our findings suggest that cell-mediated (TH1-type) immune reactions may predominate in the orbit in early GO, whereas humoral immunity (TH2-type) might play the greater role in later stages of the disease.
Aniszewski
J P
JP
Division of Endocrinology, Mayo Clinic/Foundation, Rochester, Minnesota 55905, USA.
Valyasevi
R W
RW
Bahn
R S
RS
D000273:Adipose Tissue / Q000473:pathology; D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003238:Connective Tissue / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006111:Graves Disease / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009915:Orbit / Q000473:pathology*; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000473:pathology* / Q000502:physiology; D018417:Th1 Cells / Q000473:pathology; D018418:Th2 Cells / Q000473:pathology; D013997:Time Factors
D016207:Cytokines