title: Polar glycerolipids of Chlamydomonas moewusii.
abstract: The fatty acid and polar lipid compositions of the unicellular green alga Chlamydomonas moewusii were characterized. Since this organism is an important plant model for phospholipid-based signal transduction, interest was focused on the lipids phosphatidic acid, phosphatidylinositolphosphate and phosphatidylinositolbisphosphate. A phosphatidylinositol:phosphatidylinositolphosphate: phosphatidylinositolbisphosphate ratio of 100:1.7:1.3 was found. The polyphosphoinositides accounted for 0.8 mol% of the total phospholipids and their fatty acid compositions were similar to that of phosphatidylinositol except for the enrichment of linolenic acid in phosphatidylinositol phosphate. Phosphatidic acid accounted for 0.67 mol% of the phospholipids. Major structural glycerolipids were monogalactosyldiacylglycerol (35 mol%), digalactosyldiacylglycerol (15 mol%), sulfoquinovosyldiacylglycerol (10 mol%), diacylglyceryltrimethylhomoserine (16 mol%), phosphatidylglycerol (9 mol%), phosphatidylethanolamine (8 mol%) and phosphatidylinositol (6 mol%). Relative changes in the total fatty acid compositions found during growth on nutrient-limited medium reflected mainly alterations in the compositions of the chloroplast lipids phosphatidylglycerol and monogalactosyldiacylglycerol. [32P]Pi-incorporation studies revealed that it took 6 days before the amount of label in the major phospholipids was proportional to their abundance.
authors.affiliation: Institute for Molecular Cell Biology, University of Amsterdam, The Netherlands. arisz@bio.uva.nl
authors.forename: S A
authors.initials: SA
authors.lastname: Arisz
authors.forename: J A
authors.initials: JA
authors.lastname: van Himbergen
authors.forename: A
authors.initials: A
authors.lastname: Musgrave
authors.forename: H
authors.initials: H
authors.lastname: van den Ende
authors.forename: T
authors.initials: T
authors.lastname: Munnik
mesh_terms: D000818:Animals; D002696:Chlamydomonas / Q000737:chemistry / Q000378:metabolism*; D005989:Glycerides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes
chemical_list: D005989:Glycerides; D010710:Phosphates; D010743:Phospholipids; D010761:Phosphorus Radioisotopes