title: Effect of aflatoxin on performance, hematology, and clinical immunology in lambs.
abstract: Twenty-four female lambs were intoxicated with a diet contaminated with 2 ppm aflatoxin for a period of 37 d. Twelve lambs were maintained as the control group. After this period, the lambs were left for 35 d without aflatoxin in their feed. Performance, hematology and clinical immunology were examined in the intoxicated lambs. A non-significant decrease in body weight was observed in the intoxicated lambs during the intoxication period, whereas a significant decrease (P<0.001) in average daily gain was noted on the last day of intoxication and during the clearance period. No significant differences were observed in erythrocyte count, white blood cell count or differential leukocyte count between the groups. Bacteriostatic activity of the serum was lower in the intoxicated lambs, however, there was no effect on serum opsonic activity. Phagocytosis by the neutrophils was higher during the intoxication period and the levels of IgG were elevated in the intoxicated lambs. In vivo cellular immunity was assessed by intradermal injection of phytohemagglutinin; the response was lower during intoxication period. These results indicate that a lowering in the average daily gain was the most sensitive indicator of aflatoxicosis in lambs, and that the immune response was altered, which could render the animals more susceptible to infectious diseases.
authors.affiliation: Animal Pathology Department, School of Veterinary Medicine, University of Zaragoza, Spain. afmedica@posta.unizar.es
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: M T
authors.initials: MT
authors.lastname: Verde
authors.forename: M
authors.initials: M
authors.lastname: Sanz
mesh_terms: D000348:Aflatoxins / Q000009:adverse effects* / Q000276:immunology; D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D001772:Blood Cell Count; D001835:Body Weight; D005260:Female; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000382:microbiology* / Q000473:pathology
chemical_list: D000348:Aflatoxins