2D-spatial/2D-spectral spectroscopic imaging of intracerebral gliomas in rat brain.
1H-MR spectroscopy in vivo is often hampered by poor spectral resolution. Spectral overlap can be avoided with two-dimensional spectroscopic techniques. Correlation peak imaging has been implemented to measure unambiguously the distribution of several metabolites in a rat brain glioma model. Acquisition-weighted spectroscopic imaging reduced the experimental time and provided excellent spatial localization. The choice of an appropriate spectral acquisition window granted good sensitivity. Spectroscopic images presenting a full two-dimensional spectrum in every image pixel were acquired in seven rats at 7 Tesla in 195 min, with a nominal voxel volume of 75 microl. Among other metabolites, the distribution of hypotaurine, phosphoethanolamine, alanine, and even glucose could be visualized both in the C6-glioma and in the unaffected brain.
von Kienlin
M
M
Institute of Physics, University of Würzburg, Germany. markus_f_m.von_kienlin@roche.com
Ziegler
A
A
Le Fur
Y
Y
Rubin
C
C
Dećorps
M
M
Rémy
C
C
D000818:Animals; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005910:Glioma / Q000175:diagnosis* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D009368:Neoplasm Transplantation; D051381:Rats; D012680:Sensitivity and Specificity