title: Cytosolic-Ca2+ and coxsackievirus B3-induced apoptosis in cultured cardiomyocytes of rats.
abstract: OBJECTIVE
  To explore the role of cytosolic free calcium ([Ca2+]i) in apoptosis induced by coxsackievirus B3 (CVB3) in cultured cardiomyocytes of rats.
  
  
  METHODS
  Primary cultured cardiomyocyte was prepared from Wistar rats ages 2-3 d. The apoptosis in cardiomyocyte was determined by terminated deoxynucleotide transferase directed d-UTP nick and end labeling (TUNEL) method, and the apoptosis was observed under a transmission electron microscope. [Ca2+]i in single cardiomyocyte loaded with Fluo 3-AM was measured by confocal microsorope.
  
  
  RESULTS
  (1) The concentration of CVB3 in the medium reached the peak at 24 h after CVB3 infection. (2) The apoptotic cells were not found in CVB3-infected cardiomyocyte in first 10 h, but amounted to 5% at 17 h, 60% at 24 h, and 90% at 36 h. (3) The peak value of [Ca2+]i elevation reached at 17 h after CVB3 infection (P < 0.01). (4) The characteristics of apoptosis was also seen by transmission electron microscope.
  
  
  CONCLUSIONS
  CVB3 induced the apoptosis in cultured cardiomyocyte, and [Ca2+]i mobilization was involved in the signal transduction process in apoptosis cells, and played an important role especially in the early stage of apoptosis induced by CVB3.
authors.affiliation: Department of Pharmacology, Harbin Medical University, China. liby@ems.hrbmu.edu.cn
authors.forename: B Y
authors.initials: BY
authors.lastname: Li
authors.forename: G F
authors.initials: GF
authors.lastname: Qiao
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: W H
authors.initials: WH
authors.lastname: Li
authors.forename: Z G
authors.initials: ZG
authors.lastname: Huang
authors.forename: L W
authors.initials: LW
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D029822:Enterovirus B, Human / Q000472:pathogenicity; D018613:Microscopy, Confocal; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002118:Calcium