Influence of nitrogen status on the bioconcentration of hydrophobic organic compounds to Selenastrum capricornutum.
Changes in algal nitrogen status that increase algal lipid content also affect the bioconcentration of hydrophobic organic compounds (HOCs). Bioconcentration factors (BCFs) for several HOCs increased up to nine times as the total algal lipid content of the green algae Selenastrum carpricornutum increased from 17 to 44% of the algal dry weight as a consequence of nitrogen starvation. An increase in total lipid from 17 to 44% should theoretically increase the BCFs by a factor of 2.6. BCFs for PCB 31, PCB 49, PCB 153, and DDT increased with maximum lipid content by factors of 6.3, 8.9, 8.9, and 6.6, respectively, thus more than theoretically predicted from the lipid normalization of BCFs obtained at exponential growth phase (17% total lipid for S. carpricornutum), whereas BCFs for PCB 105, phenanthrene, and 4-chloroaniline increased at 44% lipid content, only by factors of 1.5, 1.5, and 2.5, respectively, and thus less than or equal to the theoretical prediction. Lipid-class normalization of BCFs did not reveal significant information beyond that available from normalizing to total lipid.
Halling-Sørensen
B
B
Royal Danish School of Pharmacy, Section of Environmental Chemistry, Universitetsparken, Copenhagen, Denmark. bhs@mail.dfh.dk
Nyholm
N
N
Kusk
K O
KO
Jacobsson
E
E
D018533:Biomass; D000460:Chlorophyta / Q000378:metabolism*; D050356:Lipid Metabolism; D009584:Nitrogen / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000378:metabolism*
D011078:Polychlorinated Biphenyls; D009584:Nitrogen