Crystallization and preliminary X-ray diffraction studies of human epidermal growth factor.
Human epidermal growth factor (hEGF), a 6.2 kDa protein of 53 amino acids with three internal disulfide bridges, has been crystallized by the hanging-drop method. hEGF crystallizes in space group P3(1)21 (or P3(2)21) using MgCl(2) as precipitant, with unit-cell parameters a = b = 61.4, c = 87.0 A. Another type of crystal, obtained using NaCl as precipitant, belongs to a tetragonal point group and has unit-cell dimensions a = b = 102.5, c = 166.6 A. The trigonal crystals with the smaller unit cell diffract X-rays better and a native data set from a single crystal has been collected to 3.0 A resolution.
Chai
J J
JJ
Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Li
M
M
Huang
B R
BR
Luo
Y
Y
Luo
M
M
Bi
R C
RC
He
C H
CH
D003460:Crystallization; D018360:Crystallography, X-Ray; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
D011994:Recombinant Proteins; D004815:Epidermal Growth Factor