title: Synthesis of alpha-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol.
abstract: The ability of yeast to synthesize alpha-ketoglutaric acid (KGA) from ethanol has been studied. Thiamine-auxotrophic yeasts of different genera and species may be able to produce KGA; the main condition of synthesis is growth limitation by thiamine. Using a model culture, mutant Yarrowia lipolytica N 1, the principal conditions affecting KGA oversynthesis were identified. These were: thiamine concentration in medium and in cells, nitrogen and oxygen concentration in medium, and pH level. A KGA concentration of 49 g/l and a yield from ethanol consumed of 42% were achieved. Based on the results of the analysis of the activities of the key enzymes participating in ethanol metabolism and KGA synthesis, a concept of the mechanism of KGA biosynthesis by Y. lipolytica yeast is suggested and discussed.
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences, Pushchino, Moscow Region.
authors.forename: O G
authors.initials: OG
authors.lastname: Chernyavskaya
authors.forename: N V
authors.initials: NV
authors.lastname: Shishkanova
authors.forename: A P
authors.initials: AP
authors.lastname: Il'chenko
authors.forename: T V
authors.initials: TV
authors.lastname: Finogenova
mesh_terms: D002175:Candida / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids / Q000378:metabolism*; D009154:Mutation; D009584:Nitrogen / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D013831:Thiamine / Q000032:analysis; D013997:Time Factors
chemical_list: D003470:Culture Media; D007656:Ketoglutaric Acids; D000431:Ethanol; D009584:Nitrogen; D010100:Oxygen; D013831:Thiamine