title: An inductively coupled plasma method for determination of cyclophosphamide loaded to polymeric systems.
abstract: A new method for the determination of cyclophosphamide content of polyalkylcyanoacrylate nanoparticles was developed. The analyses were carried out by inductively coupled plasma atomic emission spectrometry (ICP-AES) by measuring the phosphorus content in the drug. The results obtained by this non-selective technique were compared with those given by high performance liquid chromatography (HPLC) a selective procedure that permits the detection of the cyclophosphamide molecule, and its degradation products. Sensitivity and reproducibility of both procedures were also determined. The ICP-AES method was demonstrated to be valid for sensitivity, precision, accuracy and specificity. In spite of ICP method is not a suitable procedure to analyze the degradation products of cyclophosphamide, the sensitivity of ICP is higher than chromatographic technique. Nevertheless, both procedures are appropriate for the determination of cyclophosphamide-loaded nanoparticles.
authors.affiliation: Physicochemistry Department, Faculty of Pharmacy, University of Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Salgueiro
authors.forename: M A
authors.initials: MA
authors.lastname: Egea
authors.forename: R
authors.initials: R
authors.lastname: Valls
authors.forename: M
authors.initials: M
authors.lastname: Espina
authors.forename: M L
authors.initials: ML
authors.lastname: García
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003520:Cyclophosphamide / Q000032:analysis*; D004355:Drug Stability; D011108:Polymers / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D013052:Spectrometry, X-Ray Emission / Q000379:methods
chemical_list: D018906:Antineoplastic Agents, Alkylating; D011108:Polymers; D003520:Cyclophosphamide