Determination of heterogeneous transcription start points of two c-myc genes from the common carp (Cyprinus carpio).
We determined the heterogeneous transcription start points (tsp) of two c-myc genes from the common carp (Cyprinus carpio), tetraploid teleost, by the oligo-capping method and showed the existence of the first exon. This is the first report on the existence of the first exons of the fish c-myc gene. Transcription of the two carp c-myc genes started from at least four sites in CAM1, locating from -752 to -381bp upstream of the translation start site, and from 12 sites in CAM2, locating from -586 to -413bp upstream respectively. The first introns of CAM1 and CAM2 were deduced to be 335 and 356bp, respectively. They shared 86.9% nt identity, lower than those of the second exons (94.1%), and third exons (92.3%), which suggest that the first exons evolved faster. No nt identities were found between the c-myc first exons of carp and other vertebrates. The putative promoter regions in CAM1 and CAM2 contained no obvious TATA or CCAAT boxes in the expected positions.
Futami
K
K
Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4, Minato-ku, Tokyo, Japan.
Komiya
T
T
Zhang
H
H
Okamoto
N
N
D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D005091:Exons; D016259:Genes, myc / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D020033:Protein Isoforms