Cloning and characterization of cDNA encoding zebrafish Danio rerio NM23-B gene.
A full-length zebrafish NM23-B cDNA was cloned and sequenced. The zebrafish NM23-B cDNA consists of 624bp with an open reading frame of 153 amino acids. NM23-B mRNA of approximately 0.7kb is present in adult zebrafish tissues. Zebrafish NM23-B his-tagged protein (17kDa) was produced in E. coli and characterized by binding and UV-cross-linking to a single-stranded telomeric repeat (TTAGGG)(6). This is the first report to show that fish have a NM23-H2 homologue that is similar to that in humans.
Lee
J S
JS
Institute for Molecular Biology, Seoul National University, Seoul, South Korea. jae-seong.lee@nies.jp
Lee
S H
SH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016615:Telomere / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000235:genetics*
D003432:Cross-Linking Reagents; D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D054778:NM23 Nucleoside Diphosphate Kinases; D009841:Oligonucleotides; D014157:Transcription Factors; D012313:RNA; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins