Cytotoxicity by stored human breast-milk: possible contribution of complement system.
Human milk stored over some period in vitro possesses certain cytotoxic properties, which require further studies. Cytolysis induced by stored human milk has now been further characterized, using rabbit red blood cells as targets, to determine the contribution of other components, particularly the complement system. Cytolysis was found to be temperature dependent, greatly enhanced by low concentrations of magnesium and calcium ions, but inhibited by moderate to excessive amounts of calcium ions, and by heating at 56 degrees C.
Ogundele
M O
MO
Department of Immunology, Georg-August University, Kreuzbergring 57, Göttingen, D-37075, Germany. mogundel@yahoo.com
D000818:Animals; D002021:Buffers; D002118:Calcium / Q000494:pharmacology; D003080:Cold Temperature; D003126:Colostrum / Q000737:chemistry / Q000276:immunology; D003169:Complement Inactivator Proteins / Q000494:pharmacology; D003165:Complement System Proteins / Q000276:immunology*; D003603:Cytotoxins / Q000276:immunology* / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005260:Female; D005519:Food Preservation; D006461:Hemolysis / Q000187:drug effects; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008066:Lipolysis; D008274:Magnesium / Q000494:pharmacology; D017784:Milk Banks; D008895:Milk, Human / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits
D002021:Buffers; D003169:Complement Inactivator Proteins; D003603:Cytotoxins; D005230:Fatty Acids, Nonesterified; D003165:Complement System Proteins; D008274:Magnesium; D002118:Calcium