A simple, nondestructive assay for bound hyaluronan.
A simple, convenient, nondestructive method is described for the quantitative determination of bound hyaluronan. The method is based on the binding of the cationic dye Toluidine Blue O to the D-glucuronate component of the hyaluronan repeat disaccharide. Quantification is accomplished without interference by the dye's metachromatic properties. The method is easily adapted to hyaluronan coated medical devices and should be useful to developers and manufacturers of such devices and coatings.
Johnston
J B
JB
Beacon Research/Biocoat, Inc., P.O. Box 277, Glenside, Pennsylvania 19038, USA.
D001672:Biocompatible Materials; D004187:Disaccharides / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis* / Q000737:chemistry; D019904:Polymethyl Methacrylate; D015203:Reproducibility of Results; D012997:Solvents; D013053:Spectrophotometry / Q000379:methods; D014048:Tolonium Chloride
D001672:Biocompatible Materials; D004187:Disaccharides; D005965:Glucuronates; D012997:Solvents; D014048:Tolonium Chloride; D006820:Hyaluronic Acid; D019904:Polymethyl Methacrylate