Up-regulation of sigma(1) receptor mRNA in rat brain by a putative atypical antipsychotic and sigma receptor ligand.
Sigma(1) (sigma(1)) receptor mRNA expression was studied in the prefrontal cortex, striatum, hippocampus and cerebellum of rat brain by northern blot and in situ hybridization. The effects of a chronic treatment with antipsychotic drugs (haloperidol and clozapine), and with E-5842, a sigma(1) receptor ligand and putative atypical antipsychotic on sigma(1) receptor expression were examined. A significant increase in the levels of sigma(1) receptor mRNA in the prefrontal cortex and striatum after E-5842 administration was observed, while no apparent changes were seen with either haloperidol or clozapine. Our results suggest a long-term adaptation of the sigma(1) receptor at the level of mRNA expression in specific areas of the brain as a response to a sustained treatment with E-5842.
Zamanillo
D
D
Department of Neuropharmacology, Research Center, Laboratorio Esteve S.A., Verge de Montserrat 221, 08041, Barcelona, Spain.
Andreu
F
F
Ovalle
S
S
Pérez
M P
MP
Romero
G
G
Farré
A J
AJ
Guitart
X
X
D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D017403:In Situ Hybridization; D008024:Ligands; D008297:Male; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017480:Receptors, sigma / Q000378:metabolism*; D014230:Triazoles / Q000494:pharmacology; D015854:Up-Regulation
D014150:Antipsychotic Agents; C117757:E 5842; D008024:Ligands; D011725:Pyridines; D012333:RNA, Messenger; D017480:Receptors, sigma; D014230:Triazoles; C101491:sigma1-binding protein, rat