Effects of the antioxidants curcumin and vitamin C on cisplatin-induced clastogenesis in Wistar rat bone marrow cells.
The use of dietary antioxidants to prevent antitumor agent-induced chromosomal damage in nontumor cells is currently eliciting considerable interest. Curcumin (CMN) is a dietary antioxidant that has been reported to protect against clastogenesis in in vivo and in vitro assays. This study was undertaken to investigate the modulatory effects of CMN on cisplatin-induced chromosomal aberrations in Wistar rat bone marrow cells and whether there is any potentiation of these effects with the combination between CMN and vitamin C (VC), which has been reported to reduce the clastogenic effect of many antitumor agents in in vivo assays. Animals treated with CMN plus a single dose of cisplatin, at 18, 24 or 72 h following treatment, presented a statistically significant reduction in the total amount of chromosomal damage and in the number of abnormal metaphases. The results also indicate that the combination between antioxidants would not be effective in protecting against cisplatin-induced chromosomal damage in animals sacrificed 24 h after cisplatin treatment. Under the present experimental conditions, CMN could prevent cisplatin-induced clastogenesis by acting as a free radical scavenger.
Antunes
L M
LM
Departamento Análises Clínicas, Toxicológicas e Bromatológicas, Lab. de Bromatologia, Faculdade de Ciências Farmacêuticas de Ribeirão Prêto, Universidade de São Paulo, Av. Café s/n, 14040-903, Ribeirão Prêto, Brazil. lugreggi@gly.fcfrp.usp.br
Araújo
M C
MC
Darin
J D
JD
Bianchi
M L
ML
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects*; D002869:Chromosome Aberrations; D002945:Cisplatin / Q000008:administration & dosage / Q000633:toxicity*; D004347:Drug Interactions; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D016166:Free Radical Scavengers; D009153:Mutagens; D001205:Ascorbic Acid; D002945:Cisplatin