Variability in rDNA loci in Iberian species of the genus Zabrus (Coleoptera: Carabidae) detected by fluorescence in situ hybridization.
Fluorescence in situ hybridization (FISH) with a PCR-amplified 18S ribosomal probe was used to map rDNA loci in 19 taxa of the ground beetle genus Zabrus (2n = 47-63) from the Iberian Peninsula. A quantitative and qualitative variation has been observed among related species, subspecies, populations, and even individuals. The number of rDNA-carrying chromosomes varies from 2 to 12, and the extent of the signal from small dots to entire arms. Changes altering the number of rDNA clusters seem to be uncoupled from the variation found in the chromosome number. Mechanisms that explain the numerical variation and spreading of rDNA clusters throughout the genome within the genus Zabrus are briefly discussed. No concordance between the pattern of rDNA sites and the phylogenetic relationships as based on morphological characters has been found.
Sánchez-Gea
J F
JF
Departamento de Biología Animal, Universidad de Murcia, Spain. jgalian@fcu.um.es
Serrano
J
J
Galián
J
J
D000818:Animals; D001517:Coleoptera / Q000145:classification / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D010802:Phylogeny; D013030:Spain; D013045:Species Specificity
D004275:DNA, Ribosomal