Study of the evolution of blood and striatal levels of methyldopa: a microdialysis study in sinaortic denervated rats.
Pharmacokinetic of methyldopa (50 mg kg(-1)i.p.) was studied in anesthetized sham operated and sinoaortic denervated (SAD) rats by using the microdialysis technique. Vascular shunt probe was inserted into the carotid artery and concentric probe was placed in the striatum. Levels of methyldopa were measured by HPLC-EC. The number of animals in each group was six and normal distribution of the variables of the study was assumed. Peak concentrations in arterial blood of methyldopa were similar in both groups of rats but the elimination rate constant was 0.31+/-0.09 h(-1)for sham rats (n =6) and 1.28+/-0.31 h(-1)for SAD rats (n =6, P<0.05). Low levels of methyldopa and a more pronounced decrease were seen in striatum of sinoaortic denervated rats. In conclusion, by using a microdialysis technique, different kinetic profiles of methyldopa were observed in sham operated and sinoaortic denervated rats.
Opezzo
J A
JA
Catedra de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956, Buenos Aires, (1113), Argentina.
Höcht
C
C
Taira
C A
CA
Bramuglia
G F
GF
D000316:Adrenergic alpha-Agonists / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D000771:Anesthesia, Intravenous; D018686:Anesthetics, Intravenous; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003714:Denervation; D004563:Electrochemistry; D006339:Heart Rate / Q000187:drug effects; D007274:Injections, Intraperitoneal; D008297:Male; D008750:Methyldopa / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D017551:Microdialysis; D017072:Neostriatum / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012849:Sinoatrial Node / Q000502:physiology*; D014520:Urethane
D000316:Adrenergic alpha-Agonists; D018686:Anesthetics, Intravenous; D014520:Urethane; D008750:Methyldopa