title: Differential distribution of the tetrodotoxin-sensitive rPN4/NaCh6/Scn8a sodium channel in the nervous system.
abstract: Voltage-gated sodium channels underlie the generation of action potentials in excitable cells. Various sodium channel isoforms have been cloned, functionally expressed and distinguished on the basis of their biophysical properties or differential sensitivity to tetrodotoxin (TTX). In the present study, we have investigated the immunolocalization of the TTX-sensitive sodium channel, rPN4/NaCh6/Scn8a, in discrete areas of the rat nervous system. Thus, in naïve animals, PN4 was abundantly expressed in brain, spinal cord, dorsal root ganglia (DRG) and peripheral nerve. The presence of PN4 at the nodes of Ranvier in the sciatic nerve suggests the importance of this sodium channel in peripheral nerve conduction. In addition, the pattern of PN4 immunolabeling was determined in DRG, spinal cord and sciatic nerve in rats subjected to chronic constriction nerve injury (CCI).
authors.affiliation: Center for Biological Research, Neurobiology Business Unit, Roche Bioscience, Palo Alto, CA 94304-1397, USA. Elda.Tzoumaka@roche.com
authors.forename: E
authors.initials: E
authors.lastname: Tzoumaka
authors.forename: A C
authors.initials: AC
authors.lastname: Tischler
authors.forename: L
authors.initials: L
authors.lastname: Sangameswaran
authors.forename: R M
authors.initials: RM
authors.lastname: Eglen
authors.forename: J C
authors.initials: JC
authors.lastname: Hunter
authors.forename: S D
authors.initials: SD
authors.lastname: Novakovic
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D003251:Constriction, Pathologic; D005727:Ganglia, Spinal / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015640:Ion Channel Gating; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000378:metabolism; D012584:Sciatic Nerve / Q000378:metabolism* / Q000473:pathology; D015222:Sodium Channels / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D015222:Sodium Channels