title: Immunological characterization of the major outer membrane protein of Haemophilus somnus.
abstract: Antigens and molecular mass diversity of the Haemophilus somnus major outer membrane protein (MOMP) were investigated. The molecular mass of the MOMP of 53 strains of H. somnus varied from 43 to 33 kDa and four MOMP MAb reactivity patterns were detected in immunoblot analysis and immunodot assay. The molecular mass and MAb reactivity data were used for preliminary grouping of H. somnus strains. Disease strains fell into groups 1 and 3, including two of three Group 3 subgroups, whereas strains from asymptomatic carriers were found in all the four groups and three subgroups. Immunoblot analysis with convalescent phase serum showed strain specific reactivity with MOMPs from three isolates used to reproduce disease in cattle. The reaction with the MOMP was only detectable at dilutions of 1:100 or less, whereas the same convalescent sera showed strong reactivity at dilutions of 1:1000 (or more) with other H. somnus antigens. The data suggest that the bovine immune response to the MOMP during infection is weak and is directed to antigenically variable determinants in a strain-specific manner. This may be important in evaluating the role of the antibody response to MOMPs in protective immunity.
authors.affiliation: Department of Pathology, University of California San Diego, Medical Center, 92103-8416, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Tagawa
authors.forename: F
authors.initials: F
authors.lastname: Bastida-Corcuera
authors.forename: L B
authors.initials: LB
authors.lastname: Corbeil
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000145:classification / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002353:Carrier State / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D006190:Haemophilus / Q000145:classification / Q000276:immunology*; D006192:Haemophilus Infections / Q000276:immunology / Q000662:veterinary*; D008970:Molecular Weight
chemical_list: D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins