title: Self-assembly of soluble proteins on functionalized lipid layers: a tentative correlation between the fluidity properties of the lipid film and protein ordering.
abstract: New series of amphiphilic structures are designed to exhibit various fluidity properties when spread at the air-water interface. The influence of the molecular structure of these lipids on the process of two-dimensional (2D) crystallization of the B subunit of DNA gyrase, a soluble protein, is investigated in terms of size of the crystals produced, protein ordering, and crystallization kinetics. Whereas no difference is observed concerning the mean size of the protein 2D crystals obtained on the different lipid supports, the ultimate protein ordering observable by electron microscopy using the negative-staining technique is more regularly attained with some of these new lipids. The most interesting point results from large discrepancies in crystallization kinetics as highly-ordered protein 2D crystals form within 6-24 h depending on the lipid layer structure. Thus, these new lipids reveal of special interest when studying proteins that suffer from extended incubation time at 4 degrees C or higher temperature and lose their functionality.
authors.affiliation: Université Louis Pasteur, Laboratoire de Synthèse Bioorganique associé au CNRS, Illkirch, France. lebeau@aspirine.u-strasbg.fr
authors.forename: L
authors.initials: L
authors.lastname: Lebeau
authors.forename: S
authors.initials: S
authors.lastname: Nuss
authors.forename: P
authors.initials: P
authors.lastname: Schultz
authors.forename: P
authors.initials: P
authors.lastname: Oudet
authors.forename: C
authors.initials: C
authors.lastname: Mioskowski
mesh_terms: D003460:Crystallization; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008560:Membrane Fluidity; D011312:Pressure; D011506:Proteins / Q000737:chemistry*; D012995:Solubility; D013499:Surface Properties
chemical_list: D007202:Indicators and Reagents; D008081:Liposomes; D011506:Proteins; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II