Identification and structural analysis of a MDV gene encoding a protein kinase.
DNA sequence analysis of the BamHI-C fragment of Marek's Disease Virus (MDV) reveals the presence of a 513 amino acid open reading frame (ORF). This ORF codes for a protein with an estimated M(r) of 58,901. Comparison of the amino acid sequence with those available in the Swiss-Prot database indicates extensive homology with a protein kinase (PK) of herpes simplex virus (HSV) and varicella-zoster virus (VZV). In Northern blot hybridization, a transcript of 2.0 kb was detected in MDV (GA strain) infected duck embryo fibroblasts (DEFs). A portion of the ORF was expressed in Escherichia coli as a trpE-fusion protein and used to generate antiserum in New Zealand rabbits. This antiserum specifically detected a protein of 60 kDa in MDV serotype 1, 2 and 3 infected DEFs or chicken embryo fibroblasts (CEFs) by Western blot analysis. This ORF codes for a functional PK.
Reddy
S M
SM
Avian Disease and Oncology Laboratory, USDA-ARS, East Lansing, MI 48823, USA.
Sui
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Wu
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Lee
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D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D020134:Catalytic Domain; D002478:Cells, Cultured; D002642:Chick Embryo; D005814:Genes, Viral; D008381:Herpesvirus 2, Gallid / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D014158:Transcription, Genetic
D011494:Protein Kinases; C082380:UL13 protein, Simplexvirus