Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay.
Glucocorticoids have profound effects on macrophage function and are widely used as anti-inflammatory drugs. Glucocorticoids receptor (GR) ligand binding capacity is a major determinant of cellular glucocorticoid sensitivity. The number and affinity of GR can be measured in a whole cell binding assay using (3)H-dexamethasone. Here, we describe a rapid and simple microplate assay for GR measurement using the human promonocytic cell line THP-1.
Jansen
J
J
Department of Cell Biology, Faculty of Medicine, Vrije University, Amsterdam, The Netherlands.
Uitdehaag
B
B
Koper
J W
JW
van Den Berg
T K
TK
D002460:Cell Line; D003907:Dexamethasone / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D008024:Ligands; D009000:Monocytes / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D008024:Ligands; D011965:Receptors, Glucocorticoid; C028697:dexamethasone receptor; D003907:Dexamethasone