Potential role of growth factors and extracellular matrix in wound healing after laryngotracheal reconstruction.
Laryngotracheal reconstruction (LTR) has been used for more than 20 years to treat infants and children with subglottic stenosis. Results after pediatric LTR have been satisfactory; however, approximately 10% of children have recurrent airway narrowing after LTR. The purpose of our study was to determine whether a correlation existed between specific growth factors and extracellular matrix in patients with adequate wound healing capability as compared with patients with poor wound healing capability. Histologic sections from 27 patients who underwent LTR were cut, and immunohistochemical staining was performed for transforming growth factor-beta, platelet-derived growth factor, fibronectin, tenascin, transforming growth factor-alpha, and vascular endothelial growth factor. Results showed that patients with adequate wound healing capability had a positive correlation with vasculature fibronectin, vasculature tenascin, and stromal fibronectin. Patients with poor wound healing capability had a positive correlation with stromal vascular endothelial growth factor.
Walner
D L
DL
Department of Otolaryngology and Bronchoesophagology, Rush Presbyterian St. Lukes Medical Center, and Lutheran General Children's Hospital Park Ridge, IL, USA.
Heffelfinger
S C
SC
Stern
Y
Y
Abrams
M J
MJ
Miller
M A
MA
Cotton
R T
RT
D002648:Child; D002675:Child, Preschool; D016228:Endothelial Growth Factors / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000502:physiology; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007223:Infant; D007829:Laryngostenosis / Q000601:surgery*; D007830:Larynx / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D008222:Lymphokines / Q000502:physiology; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D011183:Postoperative Complications / Q000473:pathology / Q000503:physiopathology*; D019063:Tenascin / Q000502:physiology; D014132:Trachea / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D014135:Tracheal Stenosis / Q000601:surgery; D016211:Transforming Growth Factor alpha / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing / Q000502:physiology*
D016228:Endothelial Growth Factors; D005353:Fibronectins; D006133:Growth Substances; D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; D019063:Tenascin; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors