Purine metabolites and malondialdehyde in platelets of diabetic patients.
The concentration of some of the purine nucleotides and their metabolites together with that of malondialdehyde (MDA) have been measured in resting and stimulated platelets of type 1 and type 2 diabetic patients. While control platelets show a net decrease of guanosine triphosphate (GTP) (3.1 vs. 2.3 nmol per 10(9) platelets) and guanosine diphosphate (GDP) (3.0 vs. 2.0 nmol per 10(9) platelets) and a significant increase of adenosine (0.04 vs. 0.55 nmol per 10(9) platelets) with platelet stimulation, platelets of type 1 and type 2 diabetic patients have a lesser change of these metabolites (GTP, 2.6 vs. 2.4; GDP, 2.3 vs. 2.4; adenosine, 0.04 vs. 0.30 (P < 0.05 vs. control) nmol per 10(9) platelets in type 1 diabetics; GTP, 2.4 vs. 2.7; GDP, 2.4 vs. 2.1; adenosine, 0.08 vs. 0.32 (P < 0.05 vs. control) nmol per 10(9) platelets in type 2 diabetics). These results indicate that the change (stimulated minus resting) of GTP, GDP and adenosine in diabetic platelets is significantly different from that of controls (P < 0.001). Moreover, the amount of MDA produced during platelet activation seems to be lower than controls only in type 2 diabetes (1.81 vs. 2.86 nmol per 10(9) platelets, P < 0.05). These results seem to indicate that a difference in the pattern of platelet nucleotides could be an important feature even in well-controlled diabetes, while MDA is probably modified only in association with the late vascular complications of diabetes.
Zappacosta
B
B
Lab. Di Chimica Clinica-Policlinico A. Gemelli, Istituto di Chimica e Chimica Clinica and Centro CNR per lo Studio della Chimica dei Recettori e delle Sostanze Biologicamente Attive, Rome, Italy. b.zappacosta@uniserv.ccr.rm.cnr.it
De Sole
P
P
Persichilli
S
S
Pitocco
D
D
Marra
G
G
Ghirlanda
G
G
Giardina
B
B
D000241:Adenosine / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000293:Adolescent; D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006150:Guanine Nucleotides / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D011687:Purines / Q000378:metabolism*; D012016:Reference Values
D006150:Guanine Nucleotides; D011687:Purines; D000249:Adenosine Monophosphate; D008315:Malondialdehyde; D000241:Adenosine