title: Flow injection analysis of mercury(II) in pharmaceuticals based on enzyme inhibition and biosensor detection.
abstract: An enzymatic amperometric procedure for measurement of mercury(II) in pharmaceuticals, based on the inhibition of invertase and on a glucose electrode was studied. Analytical parameters for measurements in batch and flow injection analysis (FIA) have been optimised. Mercury(II) was detected in the 10-60 ppb range with RSD < or =2%. A sample throughput of 6 h(-1) for batch and 15 h(-1) for FIA was obtained. The total mercury(II) from thimerosal (thiomersal, sodium ethylmercurithiosalicylate) in eye-drop samples was measured with the amperometric procedure after oxidative cleavage treatment. Results for both batch and FIA procedures correlated well with atomic absorbtion spectroscopy (AAS) data.
authors.affiliation: Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanita, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Bertocchi
authors.forename: E
authors.initials: E
authors.lastname: Ciranni
authors.forename: D
authors.initials: D
authors.lastname: Compagnone
authors.forename: V
authors.initials: V
authors.lastname: Magearu
authors.forename: G
authors.initials: G
authors.lastname: Palleschi
authors.forename: S
authors.initials: S
authors.lastname: Pirvutoiu
authors.forename: L
authors.initials: L
authors.lastname: Valvo
mesh_terms: D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D005947:Glucose / Q000032:analysis; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D007202:Indicators and Reagents; D008628:Mercury / Q000032:analysis* / Q000494:pharmacology; D013054:Spectrophotometry, Atomic; D013395:Sucrose / Q000032:analysis; D043324:beta-Fructofuranosidase
chemical_list: D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D013395:Sucrose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D008628:Mercury; D005947:Glucose