Articular cartilage repair in rabbits by using suspensions of allogenic chondrocytes in alginate.
The feasibility of allogenic implants of chondrocytes in alginate gels was tested for the reconstruction in vivo of artificially full-thickness-damaged articular rabbit cartilage. The suspensions of chondrocytes in alginate were gelled by the addition of calcium chloride solution directly into the defects giving in situ a construct perfectly inserted and adherent to the subchondral bone and to the walls of intact cartilage. The tissue repair was controlled at 1, 2, 4 and 6 months after the implant by NMR microscopy, synchrotron radiation induced X-ray emission to map the sulfur of glycosaminoglycans and by histochemistry. Practically a complete repair of the defect was observed 4-6 months from the implant of the chondrocytes with the recovery of a normal tissue structure. Controls in which Ca-alginate alone was implanted developed only a fibrous cartilage.
Fragonas
E
E
Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, Italy.
Valente
M
M
Pozzi-Mucelli
M
M
Toffanin
R
R
Rizzo
R
R
Silvestri
F
F
Vittur
F
F
D000464:Alginates; D000818:Animals; D001672:Biocompatible Materials; D002358:Cartilage, Articular / Q000293:injuries / Q000473:pathology / Q000601:surgery*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000637:transplantation*; D005782:Gels; D008279:Magnetic Resonance Imaging; D008422:Materials Testing; D011817:Rabbits; D014184:Transplantation, Homologous
D000464:Alginates; D001672:Biocompatible Materials; D005782:Gels