Human papilloma virus status and chromosomal imbalances in primary cervical carcinomas and tumour cell lines.
Human papilloma virus (HPV) infection is the crucial step in the initiation of cervical carcinomas. In addition, HPV18 has been implicated in tumour progression and adverse clinical outcome. We determined the HPV types in 12 primary cervical carcinomas and 12 cell lines and compared the findings with the comparative genetic hybridisation (CGH) pattern of chromosomal alterations. The most frequent alteration was the deletion at 3p14 followed by the loss of 2q34-q36 along with 3q gain. High risk HPV types were detected in all samples except one primary tumour. In contrast to the normal distribution, HPV18 was present in 75% of cases including all cell lines. The cell lines carried a higher number of genetic alterations and a different CGH pattern for several chromosomes than the primary tumours, despite microdissection. Purely HPV18 positive cases indicated a high incidence of imbalances at specific loci with peaks of the histogram coinciding with known HPV integration sites. The study suggests that HPV infection is associated with a recurrent pattern of chromosomal changes in cervical carcinomas and that the development and progression of these alterations is triggered by integration into the host genome.
Hidalgo
A
A
Institute of Pathology, University Hospital Charité, Schumannstrasse 20-21, D-10098, Berlin, Germany.
Schewe
C
C
Petersen
S
S
Salcedo
M
M
Gariglio
P
P
Schlüns
K
K
Dietel
M
M
Petersen
I
I
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000821:virology*; D002869:Chromosome Aberrations / Q000821:virology*; D025063:Chromosome Disorders; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae; D030361:Papillomavirus Infections / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000821:virology*
D004279:DNA, Viral