A recombinant avipoxvirus HIV-1 vaccine expressing interferon-gamma is safe and immunogenic in macaques.
Complex recombinant fowlpoxvirus (rFPV) vaccines expressing both HIV-1 antigens and type 1 cytokines could facilitate the induction of cellular immunity against HIV-1. A single rFPV expressing both HIV-1gag/pol and human interferon-gamma (FPVgag/pol-IFNgamma) was constructed and assessed as a therapeutic vaccine for safety and immunogenicity in macaques (Macaca nemestrina) previously infected with HIV-1. FPV gag/pol-IFNgamma vaccinations were safe and enhanced T cell proliferative responses to Gag antigens (but not control tetanus antigens). Enhanced CTL responses to gag/pol antigens were also observed following IFNgamma expressing vaccinations. Since cellular immunity may be critical to controlling or preventing HIV-1 infection, these observations suggest that avipox vectors co-expressing IFNgamma should be further evaluated as therapeutic or preventive HIV-1 vaccines.
Kent
S J
SJ
AIDS Pathogenesis Research Unit, Macfarlane Burnet Centre for Medical Research, Yarra Bend Rd, Fairfield, Australia. kent@burnet.edu.au
Zhao
A
A
Dale
C J
CJ
Land
S
S
Boyle
D B
DB
Ramshaw
I A
IA
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D005587:Fowlpox virus / Q000235:genetics*; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics / Q000494:pharmacology*; D008254:Macaca nemestrina; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
D016915:AIDS Vaccines; D004279:DNA, Viral; D015483:HIV Antibodies; D014614:Vaccines, Synthetic; D007371:Interferon-gamma