title: Recovery of ranavirus dsDNA from formalin-fixed archival material.
abstract: The extraction and amplification of nucleic acid from formalin-fixed and paraffin-embedded tissues has become an important exercise in the collection of retrospective epidemiological data. A protocol is described that enables the extraction and amplification of dsDNA from fixed tissues within paraffin blocks and from specimens stored in 10% (aq) formalin. The procedure can be used for the examination of ranavirus DNA within archival tissues thereby providing valuable data for identifying the origin and tracing the spread of ranaviruses.
authors.affiliation: CSIRO, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: Kattenbelt
authors.forename: A D
authors.initials: AD
authors.lastname: Hyatt
authors.forename: A R
authors.initials: AR
authors.lastname: Gould
mesh_terms: D000818:Animals; D017821:Boidae / Q000821:virology; D004279:DNA, Viral / Q000302:isolation & purification*; D005404:Fixatives; D005557:Formaldehyde; D010232:Paraffin; D010471:Perches / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017995:Ranavirus / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D005404:Fixatives; D005557:Formaldehyde; D010232:Paraffin