title: Changes of the p16 gene but not the p53 gene in human chondrosarcoma tissues.
abstract: The role of two important tumour suppressor genes, p16 and p53, was evaluated in cartilaginous tumour tissues. Genomic DNA from 22 chondrosarcomas, 5 benign chondroid tumours, 1 sample of reactive proliferative cartilage and 2 samples of normal cartilage were analysed using polymerase chain reaction, single strand conformational polymorphism, DNA sequencing and methylation-specific polymerase chain reaction. The p16 gene was found to be partly methylated in 5 high-grade chondrosarcomas and homozygously deleted in 1 chondrosarcoma. Moreover, a polymorphism was detected in 3 malignant tumours, but not in benign tumours or normal cartilage. Analysis of the p53 gene revealed an unchanged structure in all samples. These findings show a role for p16, but not p53, in chondrosarcoma.
authors.affiliation: Research Center for Endocrinology and Metabolism, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, Göteborg, Sweden. julia.asp@medic.gu.se
authors.forename: J
authors.initials: J
authors.lastname: Asp
authors.forename: L
authors.initials: L
authors.lastname: Sangiorgi
authors.forename: S E
authors.initials: SE
authors.lastname: Inerot
authors.forename: A
authors.initials: A
authors.lastname: Lindahl
authors.forename: L
authors.initials: L
authors.lastname: Molendini
authors.forename: M S
authors.initials: MS
authors.lastname: Benassi
authors.forename: P
authors.initials: P
authors.lastname: Picci
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001859:Bone Neoplasms / Q000235:genetics*; D002356:Cartilage; D002648:Child; D002813:Chondrosarcoma / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D019942:Genes, p16; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA