title: Heat-stress induced modulation of protein phosphorylation in virulent promastigotes of Leishmania donovani.
abstract: In parasites such as Leishmania, the study of molecular events induced in response to heat stress is of immense interest since temperature increase is an integral part of the life cycle. Protein phosphorylation is known to control major steps of proliferation and differentiation in eukaryotic cells. Studies on intracellular signaling systems in protozoa are relatively recent. We have examined the effect of heat shock on the protein phosphorylation status in promastigotes of Leishmania donovani. The patterns of total protein phosphorylation and specific phosphorylation at tyrosine residues were examined using [32P]-orthophosphate labelling of the parasites and immunoblotting with a monoclonal anti-phosphotyrosine antibody. The major proteins of L. donovani that were phosphorylated at 24 degrees C had apparent molecular weights of 110, 105, 66-68, 55, 36-40 and 20 kDa. Heat shock (from 24 to 37 degrees C) led to a significant decrease in phosphorylation of the majority of phosphoproteins in the virulent promastigotes. On the other hand, the avirulent promastigotes did not show any decrease in protein phosphorylation on exposure to heat stress. Predominant phosphorylation at tyrosine residues was detectable in proteins of putative size 105-110 kDa in both virulent and avirulent parasites. Heat shock led to a reduction in the level of phosphotyrosine in both these proteins in the case of virulent parasites, while no such reduction was detectable in avirulent parasites. Significant modifications in the phosphorylation status of proteins in response to heat stress including that of tyrosine containing proteins, observed exclusively in virulent parasites, suggest that modulation of protein phosphorylation/dephosphorylation may play a role in signal transduction pathways in the parasite upon heat shock encountered on entering the mammalian host.
authors.affiliation: Molecular Biology Laboratory, Institute of Pathology (ICMR), New Delhi, India. salotra@vsnl.com
authors.forename: P
authors.initials: P
authors.lastname: Salotra
authors.forename: R
authors.initials: R
authors.lastname: Ralhan
authors.forename: G
authors.initials: G
authors.lastname: Sreenivas
mesh_terms: D000818:Animals; D006224:Cricetinae; D006358:Hot Temperature; D007893:Leishmania donovani / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D015800:Protozoan Proteins / Q000378:metabolism*; D015398:Signal Transduction; D014774:Virulence
chemical_list: D015800:Protozoan Proteins; D019000:Phosphotyrosine