Antigenic differences in the H proteins of canine distemper viruses.
Antigenic properties between new Japanese field isolates and vaccine strains of canine distemper virus (CDV) have been compared using four monoclonal antibodies (MAbs) (JD-5, JD-7, JD-11 and d-7) against the hemagglutinin (H) proteins of CDV. JD-5, JD-7 and JD-11 are newly established antibodies. Three MAbs, namely d-7, JD-5 and JD-11, reacted similarly to all the CDV strains examined. However, JD-7 reacted much more strongly with the vaccine strains and an old field isolate than the recent field isolates in immunofluorescence, radio immunoprecipitation and virus neutralization assays. These results indicate that an antigenic region in the H protein, concerned with neutralization and recognized by JD-7, has been altered in the recent field isolates.
Iwatsuki
K
K
Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Japan.
Tokiyoshi
S
S
Hirayama
N
N
Nakamura
K
K
Ohashi
K
K
Wakasa
C
C
Mikami
T
T
Kai
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000145:classification / Q000276:immunology; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D004216:Distemper / Q000276:immunology* / Q000821:virology; D004217:Distemper Virus, Canine / Q000145:classification / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006389:Hemagglutinins, Viral / Q000145:classification / Q000276:immunology*; D007564:Japan; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000662:veterinary; D015531:Radioimmunoprecipitation Assay / Q000662:veterinary; D014709:Vero Cells; D014765:Viral Vaccines / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D014765:Viral Vaccines