title: Human herpesvirus 8 and Epstein Barr-virus in a cutaneous B-cell lymphoma and a malignant cell line established from the blood of an AIDS patient.
abstract: Human Herpesvirus 8 (HHV-8) has been consistently associated with Primary Effusion Lymphoma (PEL or body-cavity-based lymphoma) but not with other lymphomas. This paper reports on an AIDS patient without obvious malignant effusion in body cavities but with a cutaneous lymphoma where HHV-8 and Epstein-Barr virus (EBV) were detected by PCR and electron microscopy. Both viruses were also detected in all the cells of a malignant cell line (BBG1) established from the patient's peripheral blood mononuclear cells. As in PEL and PEL-derived cell lines, both the tumor and the lines lacked B-antigen expression in immunological studies but were of the same B origin as shown by clonal immunoglobulin gene rearrangements. In contrast to other co-infected cell lines, BBG1 and subclones spontaneously expressed the HHV-8 lytic antigens p40, p27, p60 and the EBV transforming latent antigen EBNA2. These data suggest that the clinical and biological features of HHV-8-and EBV-associated lymphomas could be wider than has been described to date in PEL particularly with the in vivo presence of circulating malignant dually-infected cells engaged in a spontaneous HHV-8 lytic infection.
authors.affiliation: Laboratoire de Virologie Médicale Moléculaire (RHAP), Grenoble, France. Patrice.Morand@ujf-grenoble.fr
authors.forename: P
authors.initials: P
authors.lastname: Morand
authors.forename: M
authors.initials: M
authors.lastname: Buisson
authors.forename: H
authors.initials: H
authors.lastname: Collandre
authors.forename: B
authors.initials: B
authors.lastname: Chanzy
authors.forename: O
authors.initials: O
authors.lastname: Genoulaz
authors.forename: M J
authors.initials: MJ
authors.lastname: Bourgeat
authors.forename: N
authors.initials: N
authors.lastname: Pinel
authors.forename: P
authors.initials: P
authors.lastname: Leclercq
authors.forename: D
authors.initials: D
authors.lastname: Leroux
authors.forename: V
authors.initials: V
authors.lastname: Marechal
authors.forename: L
authors.initials: L
authors.lastname: Fritsch
authors.forename: R
authors.initials: R
authors.lastname: Ruigrok
authors.forename: J M
authors.initials: JM
authors.lastname: Seigneurin
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000150:complications / Q000473:pathology / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000209:etiology / Q000821:virology*; D016393:Lymphoma, B-Cell / Q000209:etiology / Q000821:virology*; D008969:Molecular Sequence Data; D012878:Skin Neoplasms / Q000209:etiology / Q000821:virology*; D014407:Tumor Cells, Cultured