Salicylic acid mediated by the oxidative burst is a key molecule in local and systemic responses of cotton challenged by an avirulent race of Xanthomonas campestris pv malvacearum.
We analyzed the production of reactive oxygen species, the accumulation of salicylic acid (SA), and peroxidase activity during the incompatible interaction between cotyledons of the cotton (Gossypium hirsutum) cv Reba B50/Xanthomonas campestris pv malvacearum (Xcm) race 18. SA was detected in petioles of cotyledons 6 h after infection and 24 h post inoculation in cotyledons and untreated leaves. The first peak of SA occurred 3 h after generation of superoxide (O(2)(.-)), and was inhibited by infiltration of catalase. Peroxidase activity and accumulation of SA increased in petioles of cotyledons and leaves following H(2)O(2) infiltration of cotyledons from 0.85 to 1 mM. Infiltration of 2 mM SA increased peroxidase activity in treated cotyledons and in the first leaves, but most of the infiltrated SA was rapidly conjugated within the cotyledons. When increasing concentrations of SA were infiltrated 2. 5 h post inoculation at the beginning of the oxidative burst, the activity of the apoplastic cationic O(2)(.-)-generating peroxidase decreased in a dose-dependent manner. We have shown that during the cotton hypersensitive response to Xcm, H(2)O(2) is required for local and systemic accumulation of SA, which may locally control the generation of O(2)(.-). Detaching cotyledons at intervals after inoculation demonstrated that the signal leading to systemic accumulation of SA was emitted around 3 h post inoculation, and was associated with the oxidative burst. SA produced 6 h post infection at HR sites was not the primary mobile signal diffusing systemically from infected cotyledons.
Martinez
C
C
Institut de Recherches pour le Développement, GeneTrop, UR Résistance des Plantes, BP 5045, 34032 Montpellier, France.
Baccou
J C
JC
Bresson
E
E
Baissac
Y
Y
Daniel
J F
JF
Jalloul
A
A
Montillet
J L
JL
Geiger
J P
JP
Assigbetsé
K
K
Nicole
M
M
D000640:Amitrole / Q000494:pharmacology; D018548:Cotyledon / Q000378:metabolism; D003368:Gossypium / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D010544:Peroxidases / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction; D014774:Virulence; D016959:Xanthomonas campestris / Q000254:growth & development / Q000472:pathogenicity*
D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D010544:Peroxidases; D020156:Salicylic Acid; D000640:Amitrole