Studies on the biological effects of ozone: 10. Release of factors from ozonated human platelets.
In a previous work we have shown that heparin, in the presence of ozone (O3), promotes a dose-dependent platelet aggregation, while after Ca2+ chelation with citrate, platelet aggregation is almost negligible. These results led us to think that aggregation may enhance the release of platelet components. We have here shown that indeed significantly higher amount of platelet-derived growth factor (PDGF), transforming growth factor beta1 (TGF-beta1) and interleukin-8 (IL-8) are released in a dose-dependent manner after ozonation of heparinised platelet-rich plasma samples. These findings may explain the enhanced healing of torpid ulcers in patients with chronic limb ischemia treated with O3 autohaemoteraphy (O3-AHT).
Valacchi
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G
Institute of General Physiology, University of Siena, Italy.
Bocci
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D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D019343:Citric Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000097:blood* / Q000378:metabolism; D007700:Kinetics; D010126:Ozone / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000097:blood* / Q000378:metabolism
D000925:Anticoagulants; D016209:Interleukin-8; D010982:Platelet-Derived Growth Factor; D016212:Transforming Growth Factor beta; C002113:acid citrate dextrose; D019343:Citric Acid; D000244:Adenosine Diphosphate; D010126:Ozone; D006493:Heparin; D005947:Glucose