title: Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line.
abstract: The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.
authors.affiliation: Centre for Tropical Veterinary Medicine, University of Edinburgh, Edinburgh, Scotland, United Kingdom. lsakyi@ed.ac.uk
authors.forename: L
authors.initials: L
authors.lastname: Bell-Sakyi
authors.forename: E A
authors.initials: EA
authors.lastname: Paxton
authors.forename: U G
authors.initials: UG
authors.lastname: Munderloh
authors.forename: K J
authors.initials: KJ
authors.lastname: Sumption
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D016995:Ehrlichia ruminantium / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D006357:Heartwater Disease / Q000382:microbiology*; D018884:Ixodes; D008854:Microscopy, Electron; D014617:Vacuoles / Q000382:microbiology / Q000648:ultrastructure