Glucagon-like peptide-2 increases sucrase-isomaltase but not caudal-related homeobox protein-2 gene expression.
To determine the effect of glucagon-like peptide-2 (GLP-2) on sucrase-isomaltase and caudal-related homeobox protein-2 (Cdx-2) gene expression, male Wistar rats were divided into total parenteral nutrition (TPN)-fed and GLP-2-treated, TPN-fed groups. TPN was given via a jugular line, inserted under anesthesia, for 7 days. The treatment group received 40 microg/day of GLP-2 intravenously with the TPN diet. The small intestine and colon were weighed and measured. Tissue was obtained from the jejunum, terminal ileum, and midcolon. RNA analysis, morphometry, and microdissection were performed. The weight of the small intestine of GLP-2-treated rats was greater than that of TPN-fed rats (P < 0.001). GLP-2 increased the mean metaphase arrests/crypt in both the jejunum and ileum (P < 0.001). Ileal expression of sucrase-isomaltase was increased by 1. 6-fold (P < 0.05). Jejunal expression was increased by a similar amount, although not significantly (P = 0.08). There was no change in Cdx-2 gene expression. Thus GLP-2 can maintain small intestinal morphology and function, but effects on gene expression are not mediated by gross changes in the level of the mRNA for the homeobox protein Cdx-2.
Kitchen
P A
PA
St Mark's Hospital, Harrow HA1 3UJ, United Kingdom. p.kitchen@ic.ac.uk
Fitzgerald
A J
AJ
Goodlad
R A
RA
Barley
N F
NF
Ghatei
M A
MA
Legon
S
S
Bloom
S R
SR
Price
A
A
Walters
J R
JR
Forbes
A
A
D000818:Animals; D000071616:CDX2 Transcription Factor; D003106:Colon / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects*; D053767:Glucagon-Like Peptide 2; D004763:Glucagon-Like Peptides; D018398:Homeodomain Proteins / Q000235:genetics*; D007082:Ileum / Q000502:physiology; D007583:Jejunum / Q000502:physiology; D008297:Male; D010289:Parenteral Nutrition, Total; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013394:Sucrase-Isomaltase Complex / Q000235:genetics*; D015534:Trans-Activators
D000071616:CDX2 Transcription Factor; D053767:Glucagon-Like Peptide 2; D018398:Homeodomain Proteins; D010455:Peptides; D015534:Trans-Activators; D004763:Glucagon-Like Peptides; D013394:Sucrase-Isomaltase Complex