Growth hormone treatment of breeding bulls used for artificial insemination improves fertilization rates.
To evaluate new therapeutical concepts for male subfertility, we tested the effects of exogenous recombinant bovine growth hormone (rbGH) on various endocrine and metabolic parameters both in blood and in seminal plasma of bulls. Sperm quality was assessed morphometrically and by monitoring the number of successful artificial inseminations (AIs) defined as non-return rates (NRR). Aliquots of 450 semen samples were used from each bull and each experimental period (4 wk before, 14 weeks during and 6 wk after treatment). Six out of ten sires (average age 8.4 years) were treated every two weeks with 640-mg depot formulated rbGH (Eli Lilly). Four bulls received vehicle only. Blood plasma bGH, IGF-I, insulin and glucose concentrations were increased with rbGH treatment. In seminal plasma there was no effect of rbGH treatment on fructose and citrate or on testosterone concentrations. With one exception, rbGH-treated bulls had greater IGFBP-3 concentrations in seminal plasma. Motility of spermatozoa after freezing and thawing was increased compared with pretreatment rates. Most interestingly, the number of successful AIs was increased by an average of 6.0% NRR when ejaculates from rbGH-treated bulls were used.
Sauerwein
H
H
Institute of Physiology, Research Centre for Milk and Food Weihenstephan, Technical University Munich, Weihenstephan, Germany. sauerwein@uni-bonn.de
Breier
B H
BH
Gallaher
B W
BW
Götz
C
C
Küfner
G
G
Montag
T
T
Vickers
M
M
Schallenberger
E
E
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002417:Cattle / Q000502:physiology*; D019343:Citric Acid / Q000032:analysis; D004958:Estradiol / Q000032:analysis / Q000097:blood; D005306:Fertilization / Q000187:drug effects*; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000097:blood; D005632:Fructose / Q000032:analysis; D013006:Growth Hormone / Q000032:analysis / Q000097:blood / Q000494:pharmacology*; D006854:Hydrocortisone / Q000097:blood; D007315:Insemination, Artificial / Q000662:veterinary*; D007328:Insulin / Q000032:analysis; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007986:Luteinizing Hormone / Q000032:analysis / Q000097:blood; D008297:Male; D011897:Random Allocation; D011994:Recombinant Proteins / Q000494:pharmacology; D012661:Semen / Q000502:physiology; D013081:Sperm Motility; D013739:Testosterone / Q000032:analysis / Q000097:blood
D001786:Blood Glucose; D007328:Insulin; D018972:Insulin-Like Growth Factor Binding Protein 3; D011994:Recombinant Proteins; D019343:Citric Acid; D005632:Fructose; D013739:Testosterone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone; D006854:Hydrocortisone