A transcript map of the chromosome 19q-arm glioma tumor suppressor region.
Allelic loss of the chromosome 19q arm is a frequent event in human diffuse gliomas, suggesting that it contains a tumor suppressor gene. Recent deletion mapping studies have broadly implicated a 1.6-Mb interval between D19S241E and D19S596, with a limited subset of tumors, suggesting that the region may be as narrow as 150 kb. Focusing on this smaller interval, we have used cDNA selection, exon amplification, and genomic sequencing to identify three novel transcripts (EHD2, GLTSCR1, and GLTSCR2) and to map two known genes (SEPW1 and CRX). A partial transcript map of 19 transcripts and two EST markers has been constructed for the 1.6-Mb interval D19S241E-D19S596. Ten of these transcripts, including the 5 mapped to the 150-kb deletion interval, have been examined for alterations in a panel of gliomas with allelic loss of 19q. Tumor-specific alterations have not been identified in the transcripts examined thus far. Collectively, these data should facilitate subsequent efforts to identify and characterize the remaining transcripts in the 1.6-Mb interval.
Smith
J S
JS
Division of Laboratory Genetics, Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, Rochester, MN, 55905, USA.
Tachibana
I
I
Pohl
U
U
Lee
H K
HK
Thanarajasingam
U
U
Portier
B P
BP
Ueki
K
K
Ramaswamy
S
S
Billings
S J
SJ
Mohrenweiser
H W
HW
Louis
D N
DN
Jenkins
R B
RB
D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D020451:Contig Mapping; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
D018076:DNA, Complementary