title: Inhibitory effect of ferulic acid and isoferulic acid on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in RAW264.7 cells.
abstract: We investigated the effect of ferulic acid (FA) and isoferulic acid (IFA), which are the main active components of the rhizoma of Cimicifuga heracleifolia (CH), an anti-inflammatory drug used frequently in Japanese traditional medicine, on the production of macrophage inflammatory protein-2 (MIR-2) in a murine macrophage cell line, RAW264.7, in response to respiratory syncytial virus (RSV) infection. Following the exposure of cells to RSV for 20h, the MIP-2 level in condition medium was increased to about 20 ng/ml, although this level in mock-infected cells was negligible. In the presence of either FA or IFA, RSV-infected cells reduced MIP-2 production in a dose-dependent manner. These data suggest that FA and IFA might be responsible, at least in part, for the anti-inflammatory drug effect of CH extract through the inhibition of MIP-2 production.
authors.affiliation: Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sakai
authors.forename: H
authors.initials: H
authors.lastname: Kawamata
authors.forename: T
authors.initials: T
authors.lastname: Kogure
authors.forename: N
authors.initials: N
authors.lastname: Mantani
authors.forename: K
authors.initials: K
authors.lastname: Terasawa
authors.forename: M
authors.initials: M
authors.lastname: Umatake
authors.forename: H
authors.initials: H
authors.lastname: Ochiai
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D054426:Chemokine CXCL2; D018925:Chemokines / Q000096:biosynthesis* / Q000378:metabolism; D002934:Cinnamates / Q000494:pharmacology*; D003373:Coumaric Acids / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology* / Q000821:virology; D051379:Mice; D012136:Respiratory Syncytial Viruses / Q000502:physiology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D054426:Chemokine CXCL2; D018925:Chemokines; D002934:Cinnamates; D003373:Coumaric Acids; C404915:Cxcl2 protein, mouse; C004999:ferulic acid; C008180:isoferulic acid