title: Cell fractionation analysis of human CD8 glycoprotein transport between endoplasmic reticulum, intermediate compartment and Golgi complex in tissue cultured cells.
abstract: We have set up an analytical cell fractionation procedure to dissect, by a non-morphological method, the anterograde transport of proteins from endoplasmic reticulum, intermediate compartment and Golgi complex in tissue cultured cells. Using this procedure after pulse-chase labelling of cells expressing human CD8 glycoprotein, we obtained results that: (1) support the view that the intermediate compartment is a distinct station in the export from the endoplasmic reticulum to the Golgi complex; and (2) strongly suggests that the O -glycosylation process starts after the intermediate compartment, presumably in the cis -Golgi complex.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli 'Federico II', Naples, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Erra
authors.forename: L
authors.initials: L
authors.lastname: Iodice
authors.forename: L V
authors.initials: LV
authors.lastname: Lotti
authors.forename: S
authors.initials: S
authors.lastname: Bonatti
mesh_terms: D001692:Biological Transport / Q000502:physiology; D016827:CD8 Antigens / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D002451:Cell Compartmentation / Q000502:physiology; D002458:Cell Fractionation / Q000379:methods*; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008854:Microscopy, Electron; D013696:Temperature; D014162:Transfection
chemical_list: D016827:CD8 Antigens; D006023:Glycoproteins