Inhibition of N-acetyltransferase activity and DNA-2-aminofluorene adducts by glycyrrhizic acid in human colon tumour cells.
Glycyrrhizic acid (GA) was tested for inhibition of arylamine N-acetyltransferase (NAT) activity in a human colon tumour (adenocarcinoma) cell line (colo 205). Two assay systems were performed, one with cellular cytosols (9000g supernatant), the other with intact colon tumour cell cultures. The NAT activity in a human colon tumour cell line was inhibited by GA in a dose-dependent manner in both types of systems examined. The data also indicated that GA decreased the apparent values of K(m) and V(max) of NAT enzymes from human colon tumour cells in both examined systems. The DNA-2-aminofluorene adduct formation in human colon tumour cells were inhibited by GA. This report is the first to demonstrate that GA does inhibit human colon tumour cell NAT activity and DNA adduct formation.
Chung
J G
JG
Department of Microbiology, China Medical College, No. 91, Hsueh-Shih Road, Taichung, Taiwan.
Chang
H L
HL
Lin
W C
WC
Wang
H H
HH
Yeh
C C
CC
Hung
C F
CF
Li
Y C
YC
D010129:4-Aminobenzoic Acid / Q000494:pharmacology; D001191:Arylamine N-Acetyltransferase / Q000187:drug effects / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000473:pathology*; D018736:DNA Adducts; D004305:Dose-Response Relationship, Drug; D005449:Fluorenes / Q000494:pharmacology*; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D006801:Humans; D013473:Sunscreening Agents / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D002273:Carcinogens; D018736:DNA Adducts; D005449:Fluorenes; D013473:Sunscreening Agents; C012177:2-aminofluorene; D019695:Glycyrrhizic Acid; D001191:Arylamine N-Acetyltransferase; D010129:4-Aminobenzoic Acid