Amino acid sequence of an unique ribonuclease with a C-terminus rich in O-glycosylated serine and threonine from culture medium of Lentinus edodes.
The mushroom Lentinus edodes produces three base-non-specific and acid ribonucleases, RNases Le2, Le37, and Le45. The latter two are excreted from mycelia into the medium. The primary structure of RNase Le37, which had a molecular mass of 37 kDa, was sequenced. It was a member of the RNase T2 family, as is RNase Le2. RNase Le37 was some 30 amino acid residues longer at the C-terminal end than RNase Le2. The C-terminal region of RNase LE37 was rich in O-glycosylated serine and threonine. In fungal glucoamylases and chitinases, which hydrolyze raw-starch and chitin, respectively, have structures resembling the structure of the C-terminal of RNase Le37.
Inokuchi
N
N
Department of Microbiology, College of Pharmacy, Nihon University, Chiba, Japan. inokun@pha.nihon-u.ac.jp
Kobayashi
H
H
Hara
J
J
Itagaki
T
T
Koyama
T
T
Iwama
M
M
Ohgi
K
K
Irie
M
M
D000363:Agaricales / Q000201:enzymology*; D000595:Amino Acid Sequence; D003470:Culture Media; D004722:Endoribonucleases / Q000032:analysis; D006031:Glycosylation; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000737:chemistry*; D012694:Serine / Q000032:analysis*; D013912:Threonine / Q000032:analysis*
D003470:Culture Media; D013912:Threonine; D012694:Serine; D004722:Endoribonucleases; D012260:Ribonucleases; C075842:ribonuclease Rh