Nucleotide sequence of the bla(RTG-2) (CARB-5) gene and phylogeny of a new group of carbenicillinases.
We determined the nucleotide sequence of the bla gene for the Acinetobacter calcoaceticus beta-lactamase previously described as CARB-5. Alignment of the deduced amino acid sequence with those of known beta-lactamases revealed that CARB-5 possesses an RTG triad in box VII, as described for the Proteus mirabilis GN79 enzyme, instead of the RSG consensus characteristic of the other carbenicillinases. Phylogenetic studies showed that these RTG enzymes constitute a new, separate group, possibly ancestors of the carbenicillinase family.
Choury
D
D
Laboratoire de Biologie Moléculaire des Cellules Eucaryotes, 75014 Paris, France. paul@citi2.fr
Szajnert
M F
MF
Joly-Guillou
M L
ML
Azibi
K
K
Delpech
M
M
Paul
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D010405:Penicillinase / Q000235:genetics*; D010802:Phylogeny; D011513:Proteus mirabilis / Q000201:enzymology / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; C079379:RTG2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D010405:Penicillinase