title: Control of column temperature in reversed-phase liquid chromatography.
abstract: When separations by reversed-phase liquid chromatography (RP-LC) are carried out at temperatures other than ambient, resulting retention times and bandwidths can depend on the equipment used. As a result, an RP-LC separation that is adequate when carried out on one LC system may prove inadequate when the separation is repeated on a second system. In the present study, various temperature-related problems which can result in a failure of method transfer for non-ambient RP-LC methods were examined. Means for correcting for such effects and thereby ensuring method transferability are described.
authors.affiliation: Department of Chemistry, Linfield College, McMinnville, OR 97128, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Wolcott
authors.forename: J W
authors.initials: JW
authors.lastname: Dolan
authors.forename: L R
authors.initials: LR
authors.lastname: Snyder
authors.forename: S R
authors.initials: SR
authors.lastname: Bakalyar
authors.forename: M A
authors.initials: MA
authors.lastname: Arnold
authors.forename: J A
authors.initials: JA
authors.lastname: Nichols
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D013696:Temperature