title: PLGA microspheres phagocytosis by pig alveolar macrophages: influence of poly(vinyl alcohol) concentration, nature of loaded-protein and copolymer nature.
abstract: The study aimed to investigate on a pig alveolar macrophage culture model the influence of: (1) poly(D,L-lactide-co-glycolide) characteristics, (2) the residual poly(vinyl alcohol) (PVA) and (3) the nature of encapsulated proteins, immunoglobulin Y (IgY) or bovine serum albumin, on the microspheres phagocytosis efficiency. The phagocytosis evaluation was performed by flow cytometry and has allowed a screening of microspheres formulations. The hydrophilicity of microspheres resulting from the nature of the polymer and/ or from the residual hydrophilic surface agent (PVA) led to a decrease of phagocytosis intensity. The phagocytosis results of IgY-loaded microspheres strongly suggested that the phagocytosis was increased when the phagocytic cell possessed a receptor for this protein on its surface.
authors.affiliation: Laboratoire de Pharmacie Galénique, Biopharmacie et Pharmacie Clinique, Université de Rennes, France. galenic@univ-rennes1.fr
authors.forename: A M
authors.initials: AM
authors.lastname: Torche
authors.forename: P
authors.initials: P
authors.lastname: Le Corre
authors.forename: E
authors.initials: E
authors.lastname: Albina
authors.forename: A
authors.initials: A
authors.lastname: Jestin
authors.forename: R
authors.initials: R
authors.lastname: Le Verge
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000008:administration & dosage; D019344:Lactic Acid / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008863:Microspheres; D010587:Phagocytosis; D011100:Polyglycolic Acid / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013552:Swine
chemical_list: C016512:IgY; D007136:Immunoglobulins; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D011142:Polyvinyl Alcohol