title: Interleukin-1beta induction of the chemokine RANTES promoter in the human astrocytoma line CH235 requires both constitutive and inducible transcription factors.
abstract: The chemokine RANTES is an important mediator of inflammatory processes. In this report, we describe the DNA sequence and transcription factor requirements for interleukin-1beta (IL-1beta) induction of the RANTES promoter in the human astrocytoma line CH235. RANTES promoter sequences between -278 and +55 are sufficient for IL-1beta-inducibility. In vitro DNA binding assays demonstrate constitutive binding of Sp1, HMG, Ets domain, and bZIP family members to their cognate sites in the RANTES promoter, whereas NF-kappaB and IRF-1 bind in an IL-1beta-inducible manner. IL-1beta-inducibility of the RANTES promoter requires both constitutive and inducible transcription factors. The formation of a higher order nucleoprotein complex, or 'enhanceosome', may be critical for IL-1beta induction of the RANTES promoter.
authors.affiliation: Department of Immunology, Berlex Biosciences, 15049 San Pablo Avenue, P.O. Box 4099, Richmond, CA 94804, USA. neil_miyamoto@berlex.com
authors.forename: N G
authors.initials: NG
authors.lastname: Miyamoto
authors.forename: P S
authors.initials: PS
authors.lastname: Medberry
authors.forename: J
authors.initials: J
authors.lastname: Hesselgesser
authors.forename: S
authors.initials: S
authors.lastname: Boehlk
authors.forename: P J
authors.initials: PJ
authors.lastname: Nelson
authors.forename: A M
authors.initials: AM
authors.lastname: Krensky
authors.forename: H D
authors.initials: HD
authors.lastname: Perez
mesh_terms: D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D050976:Basic-Leucine Zipper Transcription Factors; D001665:Binding Sites; D018946:Chemokine CCL5 / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D051790:G-Box Binding Factors; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D018946:Chemokine CCL5; D004268:DNA-Binding Proteins; D051790:G-Box Binding Factors; D007375:Interleukin-1; D016328:NF-kappa B; D016329:Sp1 Transcription Factor; D014157:Transcription Factors