Small heat shock proteins, IbpA and IbpB, are involved in resistances to heat and superoxide stresses in Escherichia coli.
To investigate the function of Escherichia coli small heat shock proteins, IbpA and IbpB, we constructed ibpA-, ibpB- and ibpAB-overexpressing strains and also an ibpAB-disrupted strain. The ibpA-, ibpB- and ibpAB-overexpressing strains were found to be resistant not only to heat but also to superoxide stress. However, the ibpAB-disrupted strain was not more sensitive to these stresses than the wild-type strain. The heat sensitivity of a rpoH amber mutant was partially suppressed by the overexpression of plac::ibpAB. These results suggest that IbpA and IbpB may be involved in the resistances to heat and oxidative stress.
Kitagawa
M
M
Department of Biotechnology and High Technology Research Center, Kansai University, Yamate-cho 3-3-35, Suita, Osaka, Japan.
Matsumura
Y
Y
Tsuchido
T
T
D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D012808:Sigma Factor; D013481:Superoxides / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics
D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C097578:IbpA protein, E coli; C097579:IbpB protein, E coli; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; D013481:Superoxides; D006861:Hydrogen Peroxide; D010269:Paraquat