Effects of deoxyribonucleosides and cell-stimulation on frequencies of ultraviolet-B-induced micronuclei in human peripheral blood lymphocytes.
In the present study the involvement of deoxyribonucleotides (dNTPs) in the clastogenicity of ultraviolet-B (UVB) in unstimulated peripheral blood lymphocytes (G(0)-PBLs) was investigated. This was studied by analyzing the frequency of UVB-induced micronuclei (MN), either after adding a cocktail of the four deoxyribonucleosides to the PBLs immediately after exposure to UVB, or by stimulating the cells before exposure. In total, PBLs obtained from two different donors were investigated. For both donors, it could be demonstrated that addition of deoxyribonucleosides to UVB-irradiated G(0)-PBLs resulted in a significant reduction of the clastogenic effect of UVB. A gradual reduction of the clastogenic effect of UVB could also be realized by irradiating PBLs that were progressively more stimulated with the lectin PHA before exposure. The latter finding is explained by upregulation of intracellular pool sizes of dNTPs in stimulated PBLs.
Keulers
R A
RA
MGC - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University Medical Centre, Wassenaarseweg 72, P.O. Box 9503, 2300 RA, Leiden, Netherlands.
van Teylingen
C M
CM
Tates
A D
AD
D000328:Adult; D002467:Cell Nucleus / Q000528:radiation effects; D003853:Deoxyribonucleosides / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000528:radiation effects*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D016296:Mutagenesis; D010835:Phytohemagglutinins / Q000494:pharmacology; D014466:Ultraviolet Rays
D003853:Deoxyribonucleosides; D008934:Mitogens; D010835:Phytohemagglutinins