Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors.
Basic helix-loop-helix (bHLH) transcription factors have been identified for neurons and their precursors but not for glial cells. We have identified two bHLH factors, Oligo1 and Oligo2, that are specifically expressed in zones of neuroepithelium from which oligodendrocyte precursors emerge, as well as in the precursors themselves. Expression of Oligo2 in the spinal cord precedes that of platelet-derived growth factor receptor alpha (PDGFRalpha), the earliest known marker of oligodendrocyte precursors, by several days. Ectopic expression of Oligo2 in vivo causes ectopic expression of Sox10, an HMG-box transcription factor expressed in oligodendrocyte and other glial precursors. These data identify Oligo genes as the earliest known markers of oligodendrocyte lineage determination and suggest they play a causal role in this process.
Zhou
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Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Wang
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Anderson
D J
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D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D019070:Cell Lineage / Q000235:genetics; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D005820:Genetic Testing; D018257:Helix-Loop-Helix Motifs / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000235:genetics; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics*; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000737:chemistry* / Q000166:cytology / Q000502:physiology; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology; D055757:SOXE Transcription Factors; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D014157:Transcription Factors
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D055757:SOXE Transcription Factors; D014157:Transcription Factors; C405707:OLIG1 protein, human; C494786:Olig1 protein, mouse; C512773:Olig2 protein, mouse; C527924:Sox10 protein, mouse