Simultaneous determination of cinnarizine and domepiridone maleate from tablet dosage form by reverse phase ion pair high performance liquid chromatography.
A new simple, precise, rapid and selective reverse phase ion pair high performance liquid chromatography (HPLC-RP) method has been developed for the simultaneous determination of cinnarizine (CINN) and domepiridone maleate (DOME) from tablets using acetonitrile-methanol-water-0.1 N sulfuric acid (37:10:48:5 v/v/v/v) containing sodium lauryl sulfate (0.01 M), as a mobile phase and a Machery Nagel nitrile column (10 microns, 25 cm x 4.0 mm i.d.) as the stationary phase. The flow of mobile phase through the column was kept at 1.0 ml min(-1) through out the analysis. Detection was carried out using a UV detector at 225 nm. The retention times for CINN and DOME were 4.73 and 9.41 min, respectively. The linearity range and percentage recoveries for CINN and DOME were 4 1000 and 60-750 microg ml(-1) and 99.90 and 99.60%, respectively.
Argekar
A P
AP
Department of Chemistry, The Institute of Science, Mumbai, India.
Shah
S J
SJ
D002121:Calcium Channel Blockers / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002936:Cinnarizine / Q000032:analysis*; D004294:Domperidone / Q000032:analysis*; D018492:Dopamine Antagonists / Q000032:analysis*; D004355:Drug Stability; D006634:Histamine H1 Antagonists / Q000032:analysis; D011786:Quality Control; D013607:Tablets / Q000737:chemistry*
D002121:Calcium Channel Blockers; D018492:Dopamine Antagonists; D006634:Histamine H1 Antagonists; D013607:Tablets; D002936:Cinnarizine; D004294:Domperidone