Development of an affinity-driven cross-linker: isolation of a vitamin D receptor associated factor.
A vitamin D analogue containing an affinity and a photoaffinity probe (affinity-driven cross-linker, Double Label) was synthesized. An unknown factor, associated with vitamin D receptor (VDR), was isolated from rat liver nuclear extract using a GST-VDR-ligand-binding domain fusion protein (GST-VDR-LBD), affinity labeled with Double Label, and protein-protein cross-linking by photolysis.
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Bioorganic Chemistry and Structural Biology, Department of Medicine, Boston University School of Medicine, MA 02118, USA.
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D000345:Affinity Labels / Q000138:chemical synthesis*; D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000138:chemical synthesis*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D015151:Immunoblotting; D008099:Liver / Q000648:ultrastructure; D019920:Photoaffinity Labels / Q000138:chemical synthesis*; D011485:Protein Binding; D051381:Rats; D018167:Receptors, Calcitriol / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000378:metabolism; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism; D014807:Vitamin D / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D019920:Photoaffinity Labels; D018167:Receptors, Calcitriol; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D014807:Vitamin D