Direct thin layer chromatography enantioresolution of some basic DL-amino acids using a pharmaceutical industry waste as chiral impregnating reagent.
Direct enantiomeric resolution of DL-arginine, DL-histidine, DL-lysine, DL-valine and DL-leucine into their enantiomers was achieved by thin layer chromatography (TLC) on silica gel plates impregnated with optically pure (1R, 3R, 5R)-2-azabicyclo[3,3,0]octan-3-carbo-xylic acid (0.011 M) as a chiral selector which is a waste of a pharmaceutical industry. Different combinations of acetonitrile-methanol-water were found to be successful in resolving the DL-amino acids. The spots were detected by ninhydrin (0.2% in acetone) and the detection limit was 0.66 microg.
Bhushan
R
R
Department of Chemistry, University of Roorkee, India. rbcy@rurkiu.ernet.in
Martens
J
J
Thiongo
G T
GT
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D001391:Azo Compounds / Q000737:chemistry*; D001643:Bridged Bicyclo Compounds / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004345:Drug Industry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry*; D007220:Industrial Waste; D013237:Stereoisomerism
C406276:2-azabicyclo(3,3,0)octan-3-carboxylic acid; D000596:Amino Acids; D001391:Azo Compounds; D001643:Bridged Bicyclo Compounds; D007202:Indicators and Reagents; D007220:Industrial Waste