Increased expression of cyclooxygenase-2 protein in rat lung tumors induced by N-nitrosobis(2-hydroxypropyl)amine.
Expression of cyclooxygenase (COX)-2 protein in preneoplastic and neoplastic lung lesions induced by the administration of 2000 ppm of N-nitrosobis(2-hydroxypropyl)amine (BHP) in the drinking water to Wistar male rats, was examined immunohistochemically. The majority of alveolar/bronchiolar adenomas (ADs) and all adenocarcinomas (ADCs) examined, stained positive or strongly positive for COX-2. In contrast, only a minority of alveolar/bronchiolar hyperplasias demonstrated immunoreactivity and half of the squamous cell carcinomas examined, were only weakly positive. Western blotting analysis also revealed expression of COX-2 protein in the resected ADs and ADCs. These results clearly indicate up-regulated expression of COX-2 in lung neoplastic lesions, particularly ADs and ADCs, induced by BHP in rats.
Kitayama
W
W
Department of Oncological Pathology, Cancer Center, Nara Medical University, Japan.
Denda
A
A
Yoshida
J
J
Sasaki
Y
Y
Takahama
M
M
Murakawa
K
K
Tsujiuchi
T
T
Tsutsumi
M
M
Konishi
Y
Y
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000139:chemically induced / Q000201:enzymology; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002289:Carcinoma, Non-Small-Cell Lung / Q000139:chemically induced / Q000201:enzymology*; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D004790:Enzyme Induction / Q000187:drug effects; D006130:Growth Disorders / Q000139:chemically induced / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis* / Q000378:metabolism; D008175:Lung Neoplasms / Q000139:chemically induced / Q000201:enzymology*; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D009602:Nitrosamines / Q000633:toxicity*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000201:enzymology; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D002273:Carcinogens; D007527:Isoenzymes; D008565:Membrane Proteins; D009602:Nitrosamines; C012457:diisopropanolnitrosamine; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496512:Ptgs1 protein, mouse; C496513:Ptgs1 protein, rat