Coupling of chromatographic ion-exchange reaction with change-partner reaction. Presumed mechanism on the ion-ion interaction of inorganic ions in Sephadex G-15 columns.
A sample solution containing sodium or potassium dihydrogenphosphate or disodium or dipotassium hydrogenphosphate was eluted from a Sephadex G-15 column with either sodium or potassium chloride solution in various sample-eluent systems, and every one of the four kinds of ions employed was determined in the eluate. Then, the elution profiles showed the phenomenon that a negative peak of chloride ion coexisted in the fractions of a positive peak of phosphate ion, in all sample-eluent systems employed. This phenomenon was assumed to occur by the coupled reaction of change-partner and ion-exchange reactions including ion exclusion.
Okada
T K
TK
Department of Biochemistry, Kanazawa Medical University, Uchinada, Ishikawa, Japan. tk-okada@kanazawa-med.ac.jp
Nakabayashi
Y
Y
Jisaki
F
F
D002852:Chromatography, Ion Exchange / Q000379:methods*; D007287:Inorganic Chemicals; D007477:Ions
D007287:Inorganic Chemicals; D007477:Ions