The L17 ribosomal protein of Bacillus subtilis binds preferentially to curved DNA.
We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 respectively. The overproduction of L17 from B. subtilis in Escherichia coli was shown to have a strong effect on nucleoid morphology and segregation.
Zouine
M
M
Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
Beloin
C
C
Ghelis
C
C
Le Hégarat
F
F
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins; D002455:Cell Division / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C075779:Hbsu protein, bacteria; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C032493:ribosomal protein L17