title: Effects of cold and warm ischemia on the mitochondrial oxidative phosphorylation of swine lung.
abstract: BACKGROUND
  The aim of the study was to investigate the consequence of warm and cold ischemia on lung mitochondria in order to define bioenergetic limits within lung could be suitable for pulmonary transplantation.
  
  
  METHODS
  Twenty-two pigs underwent lung harvesting after lung flush with Euro-Collins solution. Mitochondria were isolated from fresh lungs, from lungs submitted to 24 or 48 hr of cold ischemia, to 30 or 45 min of warm ischemia, and to 30 min of warm ischemia followed by 24 or 48 hr of cold ischemia. Mitochondrial oxidative phosphorylation parameters were determined in isolated mitochondria by in vitro measurement of oxygen consumption.
  
  
  RESULTS
  Relative to controls, mitochondria submitted to cold ischemia showed an alteration in the oxidoreductase activities of the respiratory chain but no membrane permeability alteration. After 48 hr of cold ischemia, there was a decrease in the yield of the oxidative phosphorylation. Thirty minutes of warm ischemia did not alter the mitochondrial respiratory parameters. However, lung submitted to 45 min of warm ischemia showed mitochondrial damage as a decrease in the oxidative phosphorylation efficiency and ADP availability but no change in the oxidoreductase activities. Relative to cold ischemia alone, 30 min of warm ischemia preceding cold ischemia promoted no significant change in the respiratory parameters.
  
  
  CONCLUSIONS
  On bioenergetic basis, lung submitted to warm ischemia could be suitable for transplantation if the warm ischemia duration does not exceed 30 min. This could be a major concern in lung procurement from non-heart beating donors.
authors.affiliation: Department of Bioenergetics, Institute of Chemistry, University Hospital of Liège, University of Liège, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Willet
authors.forename: O
authors.initials: O
authors.lastname: Detry
authors.forename: B
authors.initials: B
authors.lastname: Lambermont
authors.forename: M
authors.initials: M
authors.lastname: Meurisse
authors.forename: J O
authors.initials: JO
authors.lastname: Defraigne
authors.forename: C
authors.initials: C
authors.lastname: Sluse-Goffart
authors.forename: F E
authors.initials: FE
authors.lastname: Sluse
mesh_terms: D000818:Animals; D003080:Cold Temperature; D006358:Hot Temperature; D007511:Ischemia / Q000503:physiopathology*; D008168:Lung / Q000648:ultrastructure*; D008928:Mitochondria / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000528:radiation effects; D010101:Oxygen Consumption; D015656:Respiratory Mechanics / Q000502:physiology; D013552:Swine; D013997:Time Factors