Biotin and biocytin uptake into cultured primary calf brain microvessel endothelial cells of the blood-brain barrier.
The uptake of biotin and the closely related biocytin was characterized in primary cultures of calf brain microvessel endothelial (CBME) cells. Biotin uptake was found to be Na(+)-gradient dependent and independent of changes in the membrane potential. Concentration dependence revealed a single saturation mechanism with a K(m) of 47 microM and a V(max) of 101 pmol/min/mg. Inhibition studies demonstrated dependence on metabolic energy and the necessity for a free carboxyl group for transport activity. The anticonvulsants primidone and carbamazepine had no inhibitory effect. Biotin uptake into CBME cells is a secondary active, electroneutral, saturable and specific process. Biocytin which accumulates in biotinidase deficiency, a human congenital disorder, did not inhibit biotin uptake and was not transported into these cells. The presence of human serum with normal biotinidase activity significantly reduced biotin uptake by about 50%. Further, added biocytin was hydrolyzed to biotin, which accumulated intracellularly but to a lesser extent than added free biotin. Biotin uptake after addition of plasma of biotinidase-deficient patients was not different from that in the presence of normal serum. These results indicate that the absence of biotinidase activity in serum does not reduce blood-brain barrier transport of biotin.
Baur
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Metabolic Unit, University Children's Hospital, 4005, Basel, Switzerland. bbaur@freesurf.ch
Baumgartner
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ER
D000581:Amidohydrolases / Q000172:deficiency / Q000378:metabolism; D000818:Animals; D001710:Biotin / Q000493:pharmacokinetics*; D043523:Biotinidase; D001798:Blood Proteins / Q000494:pharmacology; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D006868:Hydrolysis; D008239:Lysine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D012964:Sodium / Q000494:pharmacology; D014316:Tritium
D001798:Blood Proteins; D014316:Tritium; D001710:Biotin; D012964:Sodium; D000581:Amidohydrolases; D043523:Biotinidase; C013411:biocytin; D008239:Lysine