title: Oxidation of an oil rich in docosahexaenoic acid compared to linoleic acid in lactating women.
abstract: BACKGROUND
  We studied the oxidation of an oil rich in docosahexaenoic acid (DHA; DHASCO((R))) in lactating mothers receiving a dietary DHA supplement or a placebo. The results were compared with the oxidation of linoleic acid.
  
  
  METHODS
  Breast-feeding mothers received a dietary supplement (DHASCO; 200 mg DHA/day, n = 5) or a placebo (n = 5) for 14 days. Six weeks post partum all 10 mothers received a single dose of 2 mg/kg body weight uniformly (13)C-labeled DHASCO. In a previously reported study 6 mothers received 1 mg/kg body weight uniformly (13)C-labeled linoleic acid. Breath samples were collected over 48 h after tracer application. The total CO(2) production was measured by indirect calorimetry and the (13)C isotopic enrichment of labeled CO(2) by isotopic ratio mass spectrometry.
  
  
  RESULTS
  The oxidation of (13)C-labeled DHASCO in the supplemented and placebo groups was similar. Maximal (13)C enrichment was reached earlier in the group receiving (13)C-DHASCO (median 1.0 vs. 3.0 h in the linoleic acid group). The cumulative (13)C recovery in breath was higher in the DHASCO versus the linoleic acid group until 10 h after tracer application and comparable thereafter.
  
  
  CONCLUSIONS
  The difference in oxidation of DHASCO versus linoleic acid after tracer ingestion might be partly due to a faster absorption and oxidation of shorter chain saturated fatty acids contained in DHASCO. The cumulative oxidation of DHASCO and linoleic acid 24 and 48 h after tracer ingestion is similar.
authors.affiliation: Division of Metabolic Disorders and Nutrition, Kinderklinik and Kinderpoliklinik, Dr. von Haunersches Kinderspital, Ludwig Maximilians University, Munich, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Fidler
authors.forename: T
authors.initials: T
authors.lastname: Sauerwald
authors.forename: H
authors.initials: H
authors.lastname: Demmelmair
authors.forename: A
authors.initials: A
authors.lastname: Pohl
authors.forename: B
authors.initials: B
authors.lastname: Koletzko
mesh_terms: D000328:Adult; D001944:Breath Tests; D002245:Carbon Dioxide / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000493:pharmacokinetics*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007774:Lactation / Q000378:metabolism*; D019787:Linoleic Acid / Q000493:pharmacokinetics*; D010084:Oxidation-Reduction
chemical_list: D002245:Carbon Dioxide; D004281:Docosahexaenoic Acids; D019787:Linoleic Acid