Indirect determination of paracetamol in pharmaceutical formulations by inhibition of the system luminol-H2O2-Fe(CN)3-(6) chemiluminescence.
After a large drug scanning, the system Luminol-H2O2-Fe(CN)6(3-) is proposed for first time for the indirect determination of paracetamol. The method is based on the oxidation of paracetamol by hexacyanoferrate (III) and the subsequent inhibitory effect on the reaction between luminol and hydrogen peroxide. The procedure resulted in a linear calibration graph over the range 2.5-12.5 microg ml(-1) of paracetamol with a sample throughput of 87 samples h(-1). The influence of foreign compounds was studied and, the method was applied to determination of the drug in three different pharmaceutical formulations.
Alapont
A G
AG
Departamento de Química, Colegio Universitario CEU San Pablo, Moncada, Valencia, Spain.
Zamora
L L
LL
Calatayud
J M
JM
D000082:Acetaminophen / Q000032:analysis*; D018712:Analgesics, Non-Narcotic / Q000032:analysis*; D002021:Buffers; D004338:Drug Combinations; D005292:Ferricyanides; D017022:Flow Injection Analysis / Q000379:methods; D006861:Hydrogen Peroxide; D008163:Luminescent Measurements; D008165:Luminol; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013607:Tablets / Q000032:analysis
D018712:Analgesics, Non-Narcotic; D002021:Buffers; D004338:Drug Combinations; D005292:Ferricyanides; D004364:Pharmaceutical Preparations; D013607:Tablets; D000082:Acetaminophen; D008165:Luminol; D006861:Hydrogen Peroxide; C028033:potassium ferricyanide