[The effect of heat shock on the formation and composition of actinomycins].
The effect of various conditions of heat shock on production of actinomycins by Streptomyces chrysomallus 2 and their composition was studied. The actinomycin biosynthesis was shown to be the function of the growing mycelium and changed in accordance with changes in the volume of the mycelium and its morphological features after heat shock at various suboptimal temperatures. The temperature shock had a specific action on the antibiotic synthesis: the index of the actinomycin maximum quantity increased after the heat shock at 35 and 38 degrees C and lowered more sharply than that of the biomass volume after the heat shock at the temperatures of 40, 42, 45 and 50 degrees C for 1 hour. After the shock at 38 degrees C the component composition of the actinomycin complex did not significantly change while with addition of exogenic amino acids such as L-valine, L-leucine and L-isoleucine the shock effect on the component composition of the actinomycin complex was marked.
Liuĭ
Kh
Kh
M. V. Lomonosov Moscow State University.
Nefelova
M V
MV
Zakharchuk
L M
LM
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D002854:Chromatography, Paper / Q000706:statistics & numerical data; D003470:Culture Media; D003609:Dactinomycin / Q000032:analysis / Q000096:biosynthesis*; D018869:Heat-Shock Response / Q000502:physiology*; D013302:Streptomyces / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D000900:Anti-Bacterial Agents; D003470:Culture Media; D003609:Dactinomycin