Effects of chondroitin sulfate-C on articular cartilage destruction in murine collagen-induced arthritis.
The effects of chondroitin sulfate-C (CAS 25322-46-7, Chondroitin ZS Tab) on type II collagen (CII)-induced arthritis (CIA) in mice were evaluated. DBA/1J mice were immunized with bovine CII emulsified in Freund's complete adjuvant, followed by a booster injection 21 days later. Chondroitin sulfate-C at doses of 100, 300 and 1000 mg/kg was administered orally once daily beginning 14 days before initial immunization. An arthritis index and hind paw edema were examined from day 0 to day 49, when the mice were killed by ether anesthesia for histopathological examination. The delayed-type hypersensitivity (DTH) reaction, serum anti-CII antibody titer, and histopathologic characteristics of both synovitis and destruction of articular cartilage were analyzed. Both the arthritis index and the serum anti-CII antibody titer were reduced by treatment with chondroitin sulfate-C in a dose-dependent manner. Chondroitin sulfate-C (1000 mg/kg) significantly inhibited hind paw edema, synovitis and destruction of the articular cartilage, but not DTH reaction.
Omata
T
T
Department of Applied Research, Zeria Pharmaceutical Co., Ltd., Saitama, Japan. ken-yakuri@zeriaco.jp
Itokazu
Y
Y
Inoue
N
N
Segawa
Y
Y
D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control*; D002358:Cartilage, Articular / Q000473:pathology*; D002809:Chondroitin Sulfates / Q000627:therapeutic use*; D003094:Collagen / Q000097:blood; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D006968:Hypersensitivity, Delayed / Q000473:pathology / Q000517:prevention & control; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D013585:Synovitis / Q000473:pathology / Q000517:prevention & control
D002809:Chondroitin Sulfates; D003094:Collagen