Visualization of the in vivo generation of donor antigen-specific effector CD8+ T cells during mouse cardiac allograft rejection: in vivo effector CD8+ T cell generation during allograft rejection.
BACKGROUND
An adoptive transfer system was used to study the fate of alloreactive CD8+ H-2Kb-specific TCR transgenic (DES+) T cells in vivo after transplantation.


METHODS
A trace population of 2.0x10(6) CD8+DES+ T cells were adoptively transferred into syngeneic CBA.Ca (H-2k) mice 24 hr before transplantation of an H-2Kb+ or H-2Kb- cardiac allograft.


RESULTS
H-2Kb specific T cells proliferated and produced interleukin-2 and interferon-gamma in response to H-2Kb+, but not H-2Kb- cardiac allografts. CD8+DES+ T cells that infiltrated the H-2Kb+ cardiac allografts developed a distinct cell surface and cytokine phenotype compared with the CD8+DES+ T cells that remained in the periphery. H-2Kb-specific graft infiltrating T cells (a) underwent a larger number of cell divisions (> =3), (b) increased in size, (c) up-regulated CD69, and (d) down-regulated CD62L.


CONCLUSIONS
These results demonstrate that alloantigen-specific T cells can be monitored in vivo during the immune response to an allograft and that the fate of CD8+ T cells specific for the allogeneic class I molecules expressed by the graft is different between cells in the periphery and those that infiltrate the graft.
Gilot
B J
BJ
Nuffield Department of Surgery, University of Oxford, Headington, United Kingdom.
Hara
M
M
Jones
N D
ND
van Maurik
A
A
Niimi
M
M
Hadjianastassiou
V
V
Morris
P J
PJ
Wood
K J
KJ
D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology; D002455:Cell Division / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D000939:Epitopes; D005434:Flow Cytometry; D006084:Graft Rejection / Q000378:metabolism / Q000473:pathology; D006085:Graft Survival / Q000276:immunology; D016027:Heart Transplantation / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000187:drug effects; D015759:Ionomycin / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014019:Tissue Donors; D014184:Transplantation, Homologous / Q000473:pathology
D016207:Cytokines; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D015759:Ionomycin; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate