title: Protein interactions of SGP, an essential Streptococcus mutans GTPase, revealed by biochemical and yeast two-hybrid system analyses.
abstract: SGP, a Streptococcus mutans essential GTPase, plays a role in the stress response of the organism. Recently, we proposed that one of the physiological functions of the SGP is the modulation of the GTP/GDP ratio under different growth conditions. In order to further determine the functions of SGP and its possible interactions with other molecules, we carried out immunoprecipitation, SGP binding, and the yeast two-hybrid system analyses. These approaches suggest that SGP may oligomerize and such interactions could be important for the function of this regulatory protein.
authors.affiliation: Department of Oral Biology, State University of New York, 3435 Main Street, Buffalo, NY 14214, USA.
authors.forename: D
authors.initials: D
authors.lastname: Baev
authors.forename: S H
authors.initials: SH
authors.lastname: Ohk
authors.forename: H K
authors.initials: HK
authors.lastname: Kuramitsu
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D058619:Maltose-Binding Proteins; D010957:Plasmids / Q000235:genetics; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013295:Streptococcus mutans / Q000235:genetics* / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins; C094290:SGP protein protein, Streptococcus mutans; D019204:GTP-Binding Proteins