Ultraviolet spectrometric determination of neutral monosaccharides by HPLC with ethanolamine.
The reaction between ethanolamine and sugars in a borate solution gave intense UV absorption at 310 nm. By adding ethanolamine in the mobile phase, simple and sensitive ion-exchange HPLC of sugar-borate complexes was achieved. This method can be used to measure as little as 100 pmole of seven monosaccharides from pectic polysaccharides.
Nakamura
A
A
Hannan R&D Center, Fuji Oil Co. Ltd., Osaka, Japan. 940080@so.fujioil.co.jp
Hatanaka
C
C
Nagamatsu
Y
Y
D002851:Chromatography, High Pressure Liquid; D019856:Ethanolamine; D009005:Monosaccharides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D009005:Monosaccharides; D019856:Ethanolamine