Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and leukemia inhibitory factor (LIF) protect the repopulating ability of purified murine hematopoietic stem cells in serum-deprived cultures stimulated with SCF and IL-3.
The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1 alpha or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1 alpha and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.
Tanosaki
R
R
Laboratory of Hematopoietic Growth Factors, Lindsley F. Kimball Research Institute, New York Blood Center, New York, USA.
Ashihara
E
E
Alessandrini
L
L
Migliaccio
G
G
Migliaccio
A R
AR
D000704:Analysis of Variance; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D054407:Chemokine CCL4; D016895:Culture Media, Serum-Free; D005260:Female; D005838:Genotype; D006131:Growth Inhibitors / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D007377:Interleukin-3 / Q000494:pharmacology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013997:Time Factors
D054407:Chemokine CCL4; D016895:Culture Media, Serum-Free; D006131:Growth Inhibitors; D007377:Interleukin-3; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D019402:Macrophage Inflammatory Proteins; D011994:Recombinant Proteins; D019089:Stem Cell Factor