Cysticercosis: towards the design of a diagnostic kit based on synthetic peptides.
Cysticercosis caused by Taenia solium is a very common disease in developing countries that seriously affects human health. Diagnosis can only be confirmed with the aid of computerized tomography or nuclear magnetic resonance (NMR) creating obvious difficulties for epidemiological studies. Reliable immunoassays employing cerebrospinal fluid (CSF) have been developed, based on the use of cysticercal antigens. However, the reliance on parasite material is restrictive. Herein, we report the advances in the design of a diagnostic kit based on immunodominant synthetic peptides, targeting four candidate epitopes KETc1, KETc12, 410 and 413 which were identified from three different clones (KETc1, 12 and 4) selected from a cDNA library of Taenia crassiceps. CSF antibodies against T. solium cysticercal antigens (TCA) as well as the four peptides were determined by enzyme-linked immunoabsorbent assays (ELISA) using two panels of CSF from patients with confirmed neurocysticercosis and other neurological diseases. In the first CSF panel which included patients with high level of antibodies against TCA, KETc12 exhibited almost the same sensitivity (87.5%) as TCA (93.7%) and 100% specificity. In the second panel of 110 CSF collected at random, two peptides (KETc1 and KETc12) exhibited sensitivities of 40 and 36% respectively, and were 100% specific.
Hernández
M
M
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF, Mexico.
Beltrán
C
C
García
E
E
Fragoso
G
G
Gevorkian
G
G
Fleury
A
A
Parkhouse
M
M
Harrison
L
L
Sotelo
J
J
Sciutto
E
E
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D000947:Antigens, Helminth / Q000276:immunology*; D003551:Cysticercosis / Q000175:diagnosis* / Q000276:immunology; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D015801:Helminth Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018985:Epitopes, B-Lymphocyte; D015801:Helminth Proteins; D016056:Immunodominant Epitopes; D010455:Peptides; D011933:Reagent Kits, Diagnostic