title: Structural studies of the O-specific polysaccharide from Plesiomonas shigelloides strain CNCTC 113/92.
abstract: The structure of the O-specific side chain of the lipopolysaccharide (LPS) of Plesiomonas shigelloides, strain CNCTC 113/92 has been investigated by NMR spectroscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry and sugar and methylation analysis. It was concluded that the polysaccharide is composed of a hexasaccharide repeating unit with the following structure: in which D-beta-D-Hepp is Dglycero-beta-Dmanno-heptopyranose and 6d-beta-D-Hep is 6-deoxy-beta-Dmanno-heptopyranose. This structure represents a novel hexasaccharide repeating unit of bacterial O-antigen that is characteristic and unique to the Plesiomonas shigelloides strain. Using the high-resolution magic angle spinning technique, 1H-NMR spectra were also obtained for the O-polysaccharide components of isolated LPS and in their original form directly on the surface of bacterial cells.
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Czaja
authors.forename: W
authors.initials: W
authors.lastname: Jachymek
authors.forename: T
authors.initials: T
authors.lastname: Niedziela
authors.forename: C
authors.initials: C
authors.lastname: Lugowski
authors.forename: E
authors.initials: E
authors.lastname: Aldova
authors.forename: L
authors.initials: L
authors.lastname: Kenne
mesh_terms: D002240:Carbohydrate Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000145:classification; D009844:Oligosaccharides / Q000737:chemistry*; D016981:Plesiomonas / Q000737:chemistry* / Q000145:classification; D012703:Serotyping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019081:O Antigens; D009844:Oligosaccharides