title: Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebrates.
abstract: To determine the molecular composition of the centrosome of a higher eukaryote, we carried out a systematic nano-electrospray tandem or MALDI mass spectrometry analysis of the polypeptides present in highly enriched preparations of immunoisolated Drosophila centrosomes. One of the proteins identified is Hsp83, a member of the highly conserved Hsp90 family including chaperones known to maintain the activity of many proteins but suspected to have other essential, unidentified functions. We have found that a fraction of the total Hsp90 pool is localized at the centrosome throughout the cell cycle at different stages of development in Drosophila and vertebrates. This association between Hsp90 and the centrosome can be observed in purified centrosomes and after treatment with microtubule depolymerizing drugs, two criteria normally used to define core centrosomal components. Disruption of Hsp90 function by mutations in the Drosophila gene or treatment of mammalian cells with the Hsp90 inhibitor geldanamycin, results in abnormal centrosome separation and maturation, aberrant spindles and impaired chromosome segregation. This suggests that another role of Hsp90 might be to ensure proper centrosome function.
authors.affiliation: Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: B M
authors.initials: BM
authors.lastname: Lange
authors.forename: A
authors.initials: A
authors.lastname: Bachi
authors.forename: M
authors.initials: M
authors.lastname: Wilm
authors.forename: C
authors.initials: C
authors.lastname: González
mesh_terms: D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000276:immunology; D016227:Benzoquinones; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002502:Centrioles / Q000737:chemistry / Q000187:drug effects; D018385:Centrosome / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D020090:Chromosome Segregation / Q000187:drug effects / Q000502:physiology; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D005455:Fluorescent Antibody Technique; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047029:Lactams, Macrocyclic; D007814:Larva / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D008938:Mitosis / Q000187:drug effects; D009154:Mutation / Q000235:genetics; D011809:Quinones / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D008941:Spindle Apparatus / Q000187:drug effects / Q000502:physiology; D014714:Vertebrates
chemical_list: D000906:Antibodies; D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D011809:Quinones; C001277:geldanamycin