title: Immobilization of antibodies onto glass wool.
abstract: The immobilization of antibodies onto solid phases in an efficient and activity-retaining form is an important goal for both research and industry. Methods have been developed for the site-directed attachment of antibodies to agarose by oxidation of the carbohydrate moieties in their Fc region. Similar attachment to silianized supports have not been as successful. Here we describe a novel combination protocol for the site-directed attachment of periodate oxidized, goat polyclonal antibodies to glass wool fibers activated with 3-aminopropyltriethoxysilane. The study demonstrates that this procedure results in effective immobilization of polyclonal antibodies that retain their antigen-binding capacity. This protocol should prove useful in the development of more efficient and effective glass-based immunosupports.
authors.affiliation: E. Katzir Biotechnology Program, Research Institute, College of Judea and Samaria, Ariel, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Nisnevitch
authors.forename: M
authors.initials: M
authors.lastname: Kolog-Gulco
authors.forename: D
authors.initials: D
authors.lastname: Trombka
authors.forename: B S
authors.initials: BS
authors.lastname: Green
authors.forename: M A
authors.initials: MA
authors.lastname: Firer
mesh_terms: D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D005898:Glass
chemical_list: D000906:Antibodies