Regulation of RAG expression in developing lymphocytes.
Proper expression of products of the recombination-activating genes (RAGs) is essential for the development of the adaptive immune system. A major advance in the past year toward understanding RAG regulation is the establishment of green fluorescent protein (GFP)-RAG indicator mouse strains. In vivo visualization of RAG expression in single cells has helped to define the cells that express RAGs in secondary lymphoid organs and revealed differential cis requirements for stage- and lineage-specific RAG expression.
Nagaoka
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Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA.
Yu
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Nussenzweig
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D000818:Animals; D000917:Antibody Formation; D019070:Cell Lineage; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015321:Gene Rearrangement / Q000502:physiology*; D017930:Genes, Reporter; D018858:Germinal Center / Q000166:cytology; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D016131:Lymphocyte Subsets / Q000378:metabolism*; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology; D014162:Transfection; D045567:VDJ Recombinases
D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; C499311:RAG2 protein, human; C499308:Rag2 protein, mouse; D011993:Recombinant Fusion Proteins; C064655:V(D)J recombination activating protein 2; C064658:RAG-1 protein; D049452:Green Fluorescent Proteins; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases