Immunization with a chimeric tobacco mosaic virus containing an epitope of outer membrane protein F of Pseudomonas aeruginosa provides protection against challenge with P. aeruginosa.
A chimeric tobacco mosaic virus (TMV) was constructed by inserting sequences representing peptide 9-14mer (TDAYNQKLSERRAN) of outer membrane (OM) protein F of Pseudomonas aeruginosa between amino acids Ser154 and Gly155 of the TMV coat protein (CP). This is the first example of TMV being used to construct a chimera containing a bacterial epitope. Mice immunized with TMV-9-14 produced anti-peptide-9-14mer-specific antibodies that reacted in whole-cell ELISA with all seven Fisher-Devlin (FD) immunotype strains of P. aeruginosa, reacted specifically by Western blotting with OM protein F extracted from all seven FD immunotypes, and were opsonic in opsonophagocytic assays. The chimeric TMV-9-14 vaccine afforded immunoprotection against challenge with wild-type P. aeruginosa in a mouse model of chronic pulmonary infection. TMV-9-14 is an excellent candidate for further development as a vaccine for possible use in humans to protect against P. aeruginosa infections.
Staczek
J
J
Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, School of Medicine in Shreveport, 1501 Kings Highway, Shreveport, LA 71130-3932, USA.
Bendahmane
M
M
Gilleland
L B
LB
Beachy
R N
RN
Gilleland
H E
HE
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002678:Chimera; D005260:Female; D007114:Immunization; D051379:Mice; D008813:Mice, Inbred ICR; D018272:Porins / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000276:immunology*
D001428:Bacterial Vaccines; C109237:OmpF protein; D018272:Porins; D014614:Vaccines, Synthetic