title: LC method development for ibuprophen and validation in different pharmaceuticals.
abstract: An isocratic LC method for the simultaneous determination of ibuprophen (IBU) and its degradation product (4-isobutylacetophenone) has been developed and validated for different pharmaceuticals (sachets, tablets and gels). The chromatographic separation was achieved with phosphoric acid solution (pH 3.2)-acetonitrile (50:50, v/v) as mobile phase, a Hypersil C18 column and UV detection at 254 nm. In all cases, sample preparation was required before HPLC analysis.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universidad Complutense, Madrid, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Gasco-Lopez
authors.forename: R
authors.initials: R
authors.lastname: Izquierdo-Hornillos
authors.forename: A
authors.initials: A
authors.lastname: Jimenez
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D007052:Ibuprofen / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen