Identification of the novel allele HLA-B*1545: assessment of alloreactivity in case of mismatch with other B*15 alleles.
The novel allele HLA-B*1545, which has been serologically typed as B62, was identified in a male Caucasian. In the sequence analysis the new allele differs from B*1507 by nucleotide 419 which is located in exon 3. Its structure suggests that it may have originated by a point mutation or by gene conversion with a variety of HLA-B alleles. At the protein level, the nucleotide substitution results in an amino add exchange compared to B*1507 (Tyr116Ser). Due to probably substantial differences to B*1507 and the other B*15 variants with regard to peptide binding, a mismatch is likely to impair clinical outcome of bone marrow transplantation.
Elsner
H A
HA
Department of Transfusion Medicine, Hannover Medical School, Germany.
Blasczyk
R
R
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D015235:HLA-B Antigens; D059910:HLA-B15 Antigen