High-performance liquid chromatographic analysis of new triazole antifungal agent SYN-2869 and its derivatives in plasma.
A simple reversed-phase high-performance liquid chromatography (HPLC) method with UV detection was developed and validated for the quantitation of SYN-2869, a novel triazole antifungal agent and its analogs in rat plasma. The method involved a simple precipitation of plasma protein with acetonitrile (1:10 ratio). The reconstituted sample after evaporation to dryness was injected onto a HPLC column. SYN-2869 and its analogs were separated from the matrix components on a symmetry C18 column using an aqueous mobile phase of acetonitrile and water with a flow rate of 1 ml min(-1). A step gradient of 40-80% acetonitrile eluted all four compounds. The run time was 30 min. The linear range was 0.5 10 microg ml(-1)(r2 > 0.999). The limit of quantitation was 0.5 microg ml(-1). The inter-day precision and accuracy for SYN-2869 standard concentration were from 1.9 to 8.5% and from 1.4 to +/- 4.40%, respectively. The precision and accuracy of intra-day quality control samples were from 4.6 to 5.2% and from 4.6 to 12%, respectively.
Khan
J K
JK
Department of Biopharmaceutics and Pharmacokinetics, SynPhar Laboratories Inc., Edmonton, Alberta, Canada.
Montaseri
H
H
Poglod
M
M
Bu
H Z
HZ
Daneshtalab
M
M
Micetich
R G
RG
D000818:Animals; D000935:Antifungal Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D051379:Mice; D015394:Molecular Structure; D010879:Piperazines / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D014230:Triazoles / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics
D000935:Antifungal Agents; D010879:Piperazines; C118964:Syn 2869; D014230:Triazoles