Novel chicken CXC and CC chemokines.
Upon stimulation with lipopolysaccharide (LPS) the chicken macrophage cell line HD-11 secretes factors with cytokine activity. To characterize these molecules, representational difference analysis with RNA of LPS-induced and uninduced HD-11 cells was performed. Two cDNA clones were isolated that code for polypeptides with structural features of chemokines. cDNA K60 codes for a novel CXC chemokine of 104 residues including a putative signal peptide of 20 amino acids at the N-terminus. It is 67% identical to the previously cloned chicken chemokine 9E3/CEF4. K60 exhibits a similar degree of sequence identity to human interleukin 8 and other related CXC chemokines (about 50%), rendering straight-forward predictions of its biological properties difficult. cDNA K203 codes for a novel CC chemokine of 89 amino acids including a putative N-terminal signal peptide of 21 residues. It is 43% identical to a previously characterized chicken protein with homology to mammalian macrophage inflammatory protein 1beta (MIP-1beta). K203 exhibits about 50% sequence identity to human MIP-1beta and other related CC chemokines.
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Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, University of Freiburg, Freiburg, 79008, Germany.
Schneider
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Staeheli
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Weining
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KC
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D019743:Chemokines, CXC / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D002645:Chickens; D018076:DNA, Complementary / Q000032:analysis; D015723:Gene Library; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D019742:Chemokines, CC; D019743:Chemokines, CXC; D018076:DNA, Complementary; D008070:Lipopolysaccharides