Description of a nonlethal herpes simplex virus type 1 glycoprotein D deletion mutant affecting a site frequently used for PCR.
We report a herpes simplex virus type 1 mutant which failed to amplify with a commonly used glycoprotein D primer set. The virus contained a nine-base deletion in the gene's 5' nontranslated region. The altered amplicon was clearly distinguishable on a 4% high-resolution agarose gel.
Coyle
P V
PV
Regional Virus Laboratory, Royal Victoria Hospital, Belfast, United Kingdom. p.coyle@qub.ac.uk
Jain
S
S
Wyatt
D
D
McCaughey
C
C
O'Neill
H J
HJ
D020121:5' Untranslated Regions; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017384:Sequence Deletion; D014759:Viral Envelope Proteins / Q000235:genetics*
D020121:5' Untranslated Regions; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1