Specific detection of membrane-toxic substances with a conductivity assay.
A conductivity assay that represents a new biotest able to detect the effects of membrane-toxic compounds, e.g., detergents, organic solvents, and radical formers, on various organisms was previously described and developed. The conductivity assay measures ion leakage from cells, tissues, or whole plant and animal organisms whose membrane systems have been damaged by membrane-toxic compounds. In this study the specificity of the conductivity assay for membrane-toxic compounds was tested by comparing the electrolyte efflux from Elodea canadensis leaves during incubation with a well-known detergent (benzalkonium chloride) using different plant physiological and biochemical techniques (photochemical efficiency, plasmolysis capacity, NBT reduction, and electron microscopy of membranes of E. canadensis leaves). The comparison of the different methods proved that the electrolyte loss during benzalkonium chloride incubation determined in the conductivity assay is due to membrane impairment. The observed electrolyte loss correlated with a reduction of photochemical efficiency and a decrease in both plasmolysis and NBT reduction capacity. Furthermore, a disintegration of the plasmalemma could be seen in the electron micrographs. These results indicate that the measured electrolyte loss in the conductivity assay is a specific effect of membrane-toxic compounds.
Eich
J
J
Institut für Biologie II/Botanik, Universität Freiburg, Freiburg i. Br., D-79104, Germany.
Dürholt
H
H
Steger-Hartmann
T
T
Wagner
E
E
D001548:Benzalkonium Compounds / Q000633:toxicity*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D003217:Conductometry; D003902:Detergents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004573:Electrolytes / Q000032:analysis / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005614:Freeze Fracturing; D008855:Microscopy, Electron, Scanning; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010575:Pesticides / Q000633:toxicity; D059828:Plant Cells; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D010944:Plants / Q000187:drug effects*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001548:Benzalkonium Compounds; D003902:Detergents; D004573:Electrolytes; D010575:Pesticides; D009580:Nitroblue Tetrazolium