Propionic and L-methylmalonic acids induce oxidative stress in brain of young rats.
The in vitro effects of propionic and L-methylmalonic acids on some parameters of oxidative stress were investigated in the cerebral cortex of 21-day-old rats. Chemiluminescence, thiobarbituric acid-reactive substances (TBA-RS) and total radical-trapping antioxidant capacity (TRAP) were measured in brain tissue homogenates in the presence of propionic or L-methylmalonic acids at concentrations ranging from 1 to 10mM. Both acids significantly increased chemiluminescence and TBA-RS and decreased TRAP, indicating a simulation of lipid peroxidation and a reduction of tissue antioxidant potential. Other organic acids tested which accumulate in some organic acidemias (suberic, sebacic, adipic, 3-methylglutaric and 4-hydroxybutyric acids) did not affect these parameters. This study provides evidence that free radical generation may participate in the neurological dysfunction of propionic and methylmalonic acidemias.
Fontella
F U
FU
Departmento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, Porto Alegre RS, Brazil.
Pulrolnik
V
V
Gassen
E
E
Wannmacher
C M
CM
Klein
A B
AB
Wajner
M
M
Dutra-Filho
C S
CS
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism*; D008163:Luminescent Measurements; D008764:Methylmalonic Acid / Q000494:pharmacology*; D018384:Oxidative Stress; D011422:Propionates / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D011422:Propionates; D017392:Thiobarbituric Acid Reactive Substances; D008764:Methylmalonic Acid; C029658:propionic acid