title: Fetal demise with Smith-Lemli-Opitz syndrome confirmed by tissue sterol analysis and the absence of measurable 7-dehydrocholesterol Delta(7)-reductase activity in chorionic villi.
abstract: Smith-Lemli-Opitz syndrome (SLOS), an autosomal recessive condition with multiple malformations, mental retardation, and growth failure, results from markedly reduced activity of the final enzyme in the cholesterol biosynthetic pathway, 7-dehydrocholesterol Delta(7)-reductase (DHCR7). We diagnosed SLOS in a fetus following intrauterine demise at 32 weeks' gestation. Chorionic villus (CV) sampling had been performed at 30 weeks because oligohydramnios and atrioventricular septal defect were noted on fetal ultrasound. On fetal post-mortem examination, a midline U-shaped soft palate cleft, micrognathia, postaxial polydactyly of the fingers with single transverse palmar creases bilaterally, and cutaneous syndactyly of toes two-three bilaterally suggested SLOS. We hypothesized that SLOS could be confirmed by analysis of tissue sterols despite extensive autolysis, and by measurement of enzyme activity in CV cells. Measurement of DHCR7 activity in CV cells was undertaken using ergosterol as a substrate. CV cells were unable to convert any ergosterol to brassicasterol after a 72 h incubation period while control CV cells reduced 12.6-71.8% of ergosterol to brassciasterol in a 72 h period. SLOS was confirmed by measurement of elevated 7-dehydrocholesterol (7-DHC) in the CV cells. Measurements of sterols were made in multiple fetal tissues. All tissues analysed showed elevated 7-DHC with markedly increased 7-DHC/cholesterol ratios.
authors.affiliation: Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland, Oregon, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Linck
authors.forename: S J
authors.initials: SJ
authors.lastname: Hayflick
authors.forename: D S
authors.initials: DS
authors.lastname: Lin
authors.forename: K P
authors.initials: KP
authors.lastname: Battaile
authors.forename: S
authors.initials: S
authors.lastname: Ginat
authors.forename: T
authors.initials: T
authors.lastname: Burlingame
authors.forename: K M
authors.initials: KM
authors.lastname: Gibson
authors.forename: M
authors.initials: M
authors.lastname: Honda
authors.forename: A
authors.initials: A
authors.lastname: Honda
authors.forename: G
authors.initials: G
authors.lastname: Salen
authors.forename: G S
authors.initials: GS
authors.lastname: Tint
authors.forename: W E
authors.initials: WE
authors.lastname: Connor
authors.forename: R D
authors.initials: RD
authors.lastname: Steiner
mesh_terms: D000328:Adult; D002784:Cholesterol / Q000032:analysis; D002824:Chorionic Villi / Q000201:enzymology*; D015193:Chorionic Villi Sampling; D003684:Dehydrocholesterols / Q000032:analysis; D004875:Ergosterol / Q000378:metabolism; D005260:Female; D005313:Fetal Death; D005865:Gestational Age; D006801:Humans; D010088:Oxidoreductases / Q000032:analysis / Q000172:deficiency* / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011247:Pregnancy; D019082:Smith-Lemli-Opitz Syndrome / Q000175:diagnosis*; D013261:Sterols / Q000032:analysis*; D016216:Ultrasonography, Prenatal
chemical_list: D003684:Dehydrocholesterols; D013261:Sterols; D002784:Cholesterol; C016705:7-dehydrocholesterol; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019995:7-dehydrocholesterol reductase; D004875:Ergosterol