Chemical cross-linking analysis of Ca(2+-)ATPase from rabbit skeletal muscle.
A student laboratory experiment is described dealing with chemical cross-linking analysis of Ca(2+-)ATPase from rabbit muscle. The experiment introduces students to a wide range of techniques including subcellular fractionation, chemical cross-linking, polyacrylamide gel electrophoresis, blotting and immunochemical detection of proteins.
Murray
BE
Department of Pharmacology, National University of Ireland, University College Dublin, Belfield, Dublin, Ireland
Ohlendieck
K