Solid phase extraction of clenbuterol from plasma using immunoaffinity followed by HPLC.
An immuno-extraction column for clenbuterol has been prepared. Optimum conditions for the selective retention and elution of clenbuterol have been developed, based on a modification of our earlier work on morphine, chlortoluron and isoproturon. Clenbuterol could be retained on the immuno-column then eluted in one x one ml fraction using 50% methanol in phosphate buffered saline pH 2. On columns containing antisera (but not to clenbuterol) the clenbuterol was removed in the washing step. HPLC-UV determination gave clean traces. Day-to-day reproducibility was improved by precipitating the plasma proteins with acetonitrile.
Rashid
B A
BA
Robens Analytical Centre, School of Biological Science, University of Surrey, Guildford, UK.
Kwasowski
P
P
Stevenson
D
D
D000318:Adrenergic beta-Agonists / Q000032:analysis / Q000097:blood* / Q000276:immunology; D000915:Antibody Affinity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis / Q000097:blood* / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000318:Adrenergic beta-Agonists; D002976:Clenbuterol