Retinal ganglion cells in the South American opossum (Didelphis aurita).
By using the Golgi technique, the authors investigated the morphology of ganglion cells in the retinas of South American opossums. In flat-mount preparations of the retinas, cell bodies, entire dendritic fields, and the stratification level of ganglion cells were studied. Fractal dimensions of dendritic trees, an objective quantitative measure of morphological complexity, were included as a morphological parameter of classification. Based on these characteristics, nineteen types of ganglion cells were described. A great number of opossum ganglion cell types had dendrites stratifying in both sublaminae of the inner plexiform layer (IPL) in five different ways (S1-S3 [G9], S1-S4 [G17 and G22], S2/S3 [G19], S2-S4 [G15, G16, G21 and G221, and S2-S5 [G61), and only two types (G8, and G10) showed narrow field dendritic trees ramifying in S4 only. Morphological types of opossum ganglion cells were compared to their counterparts in cat retina. The distribution pattern of large cell bodies on the ganglion cell layer was analyzed employing the Nissl staining method, immunocytochemistry for neurofilaments, and the reduced silver neurofibrillar staining method. The results showed a random pattern of distribution.
Moraes
A M
AM
Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde Bl.G, Universidade Federal do Rio de Janeiro, RJ, Brazil.
Oliveira
M M
MM
Hokoç
J N
JN
D000818:Animals; D002415:Cats / Q000033:anatomy & histology; D003712:Dendrites / Q000648:ultrastructure; D017709:Fractals; D006056:Golgi Apparatus / Q000648:ultrastructure; D066298:In Vitro Techniques; D009893:Opossums / Q000033:anatomy & histology*; D012165:Retinal Ganglion Cells / Q000145:classification / Q000166:cytology* / Q000648:ultrastructure