Identification of the mouse beta'-COP Golgi component as a spermatocyte autoantigen in scleroderma and mapping of its gene Copb2 to mouse chromosome 9.
In an immunological screening of a mouse testicular cDNA library with a human CREST serum we isolated five overlapping cDNA clones encoding the mouse homolog of a Golgi coatomer complex protein (accession number AF043120), designated beta'-COP in bovine and p102 in humans. We generated antibodies against this protein which specifically recognize the Golgi apparatus of mouse spermatocytes. FISH analyses assigned the beta'-COP gene Copb2 to mouse Chromosome 9, region E3-F1. Our results demonstrate that CREST sera can contain antibody components against Golgi proteins as well as against nuclear proteins.
Tarsounas
M
M
Department of Biology, York University, Toronto ON, Canada.
Heng
H H
HH
Ye
C J
CJ
Pearlman
R E
RE
Moens
P B
PB
D000818:Animals; D000918:Antibody Specificity; D001324:Autoantigens / Q000276:immunology*; D015153:Blotting, Western; D017675:CREST Syndrome / Q000276:immunology*; D003001:Cloning, Molecular; D020756:Coatomer Protein / Q000235:genetics*; D015723:Gene Library; D006056:Golgi Apparatus / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D017386:Sequence Homology, Amino Acid; D013090:Spermatocytes / Q000166:cytology / Q000276:immunology*
D001324:Autoantigens; D020756:Coatomer Protein; D007106:Immune Sera