Persistence of human mitochondrial DNA throughout the development to the blastocyst of mouse zygotes microinjected with human mitochondria.
The conditions for transfer of human mitochondria into fertilised mouse ova were elaborated. Species-specific primers were designed to discriminate human mitochondrial DNA (mtDNA) and the endogenous mtDNA in the preimplantation embryos. Human mitochondria isolated from the HepG2 cell line were microinjected into murine zygotes, and the latter cultured for 96 h to the blastocyst stage. The polymerase chain reaction allowed the detection of human mtDNA at every stage of embryo cleavage. In some cases a clear disparity in distribution of human mtDNA among blastomeres was evident.
Vasilyev
V B
VB
Department of Molecular Genetics, Institute for Experimental Medicine, Saint Petersburg, Russia. vadim@biomed.spb.su
Sokolova
V A
VA
Sorokin
A V
AV
Bass
M G
MG
Arbuzova
N I
NI
Patkin
E L
EL
Golubkov
V I
VI
Dyban
A P
AP
Gaitskhoki
V S
VS
D000818:Animals; D001755:Blastocyst / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D008845:Microinjections; D016133:Polymerase Chain Reaction; D015053:Zygote / Q000378:metabolism*
D004272:DNA, Mitochondrial