title: Decreased production of interleukin-6 and prostaglandin E2 associated with inhibition of delta-5 desaturation of omega6 fatty acids in mice fed safflower oil diets supplemented with sesamol.
abstract: The differences in the immune responses in mice fed sesame oil diets and those fed sesamin may be attributed to the presence of other lignans in the non-fat portion of the oil. The fatty acid composition (mean +/- SD mol. %) of liver membrane phospholipids and the levels of endotoxin-induced prostaglandin (PG) E2, interleukin (IL)-6, IL-10, IL-12 and tumor necrosis factor (TNF)-alpha were determined in mice fed diets supplemented with 5% safflower oil (SO) in the absence or presence of 1% sesamol. The levels of dihomo-gamma-linolenic acid (20:3omega6) were markedly higher (P<0.025) in the livers from mice fed sesamol supplemented SO diets (1.6 +/- 0.1) compared to the controls (1.4 +/- 0.1). These data suggest that sesamol or its metabolite could inhibit the in vivo delta-5 desaturation of omega6 fatty acids. Further, in animals fed sesamol supplemented SO diets, the levels of PGE2 (228 +/- 41 pg/ml) were markedly lower (P<0.01) compared to those fed SO diet alone (1355 +/- 188 pg/ml). Concomitantly, the concentrations of IL-6 were also lower (P<0.01) in mice fed sesamol diet (63 +/- 11 ng/ml) compared to the controls (143 +/- 22 ng/ml). A marked reduction in the levels of PGE2 in animals fed sesamol diets suggests that sesamol or its metabolite could inhibit the activity of cyclooxygenase enzyme.
authors.affiliation: Harvard Institutes of Medicine, Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Chavali
authors.forename: R A
authors.initials: RA
authors.lastname: Forse
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D052117:Benzodioxoles; D000090943:Delta-5 Fatty Acid Desaturase; D015232:Dinoprostone / Q000096:biosynthesis*; D044943:Fatty Acid Desaturases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005260:Female; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis*; D019787:Linoleic Acid / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D008566:Membranes / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010636:Phenols / Q000494:pharmacology*; D012450:Safflower Oil / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D052117:Benzodioxoles; D000090943:Delta-5 Fatty Acid Desaturase; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D015850:Interleukin-6; D010636:Phenols; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D018664:Interleukin-12; D016718:Arachidonic Acid; D012450:Safflower Oil; C025583:sesamol; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases; D015232:Dinoprostone