Transtracheal administration of interleukin-12 induces neutrophil responses in the murine lung.
Although the roles of interleukin-12 (IL-12) in the immunomodulation of antigen-specific responses are well characterized, the effects of IL-12 on the respiratory tract following mucosal administration are not well defined. Therefore, we investigated changes in the murine lung shortly after intranasal (i.n.) administration of murine IL-12. We showed that IL-12 induced neutrophil influx to the murine lung in both C57BL/6 and BALB/c mice. Histologic examination revealed that intranasal administration of IL-12 with liposomes induced focal neutrophil infiltration into the alveoli and a significant increase in neutrophils in bronchoalveolar lavage fluids when compared with administration of liposomes alone. In vitro chemotaxis assays indicated that the observed pulmonary neutrophil response induced by IL-12 could have been due in part to the direct chemotactic activity of IL-12 for murine neutrophils.
Papp
Z
Z
Veterinary Infectious Disease Organization, Department of Veterinary Pathology, Western College of Veterinary Medicine, Saskatoon, Canada.
Middleton
D M
DM
Røntved
C M
CM
Foldvari
M
M
Gordon
J R
JR
Baca-Estrada
M E
ME
D000281:Administration, Intranasal; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D018664:Interleukin-12 / Q000008:administration & dosage*; D008081:Liposomes; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014132:Trachea
D008081:Liposomes; D018664:Interleukin-12