title: Locomotion and proliferation of glioblastoma cells in vitro: statistical evaluation of videomicroscopic observations.
abstract: OBJECTIVE
  The motility and doubling of human glioblastoma cells were investigated by means of statistical evaluation of large sets of data obtained using computer-aided videomicroscopy.
  
  
  METHODS
  Data were obtained on cells in four established glioblastoma cell lines and also on primary tumor cells cultured from fresh surgical samples. Growth rates and cell cycle times were measured in individual microscopic fields. The averages of cell cycle time and the duplication time for the recorded cell populations were 26.2 +/- 5.6 hours and 38 +/- 4 hours, respectively. With these parameters, no significant differences among the cell lines were revealed. Also, there was no correlation in the cell cycle time of a parent cell and its progeny in any of the cultures. Statistical analysis of cell locomotion revealed an exponential distribution of cell velocities and strong fluctuations in individual cell velocities across time. The average velocity values ranged from 4.2 to 27.9 micro/hour. In spite of the uniform histopathological classification of the four tumors, each cell line produced by these tumors displayed distinct velocity distribution profiles and characteristic average velocity values. A comparison of recently established primary cultures with cell lines that had propagated multiple times indicated that cells derived from different tumors sustain their characteristic locomotor activity after several passages.
  
  
  CONCLUSIONS
  It can be inferred from the data that statistical evaluation of physical parameters of cell locomotion can provide additional tools for tumor diagnosis.
authors.affiliation: Department of Biological Physics, Eotvos University, Budapest, Hungary. hebal@biol-phys.elte.hu
authors.forename: B
authors.initials: B
authors.lastname: Hegedüs
authors.forename: A
authors.initials: A
authors.lastname: Czirók
authors.forename: I
authors.initials: I
authors.lastname: Fazekas
authors.forename: T
authors.initials: T
authors.lastname: B'abel
authors.forename: E
authors.initials: E
authors.lastname: Madar'asz
authors.forename: T
authors.initials: T
authors.lastname: Vicsek
mesh_terms: D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D003627:Data Interpretation, Statistical; D004867:Equipment Design; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D066298:In Vitro Techniques; D018715:Microscopy, Video / Q000295:instrumentation; D014407:Tumor Cells, Cultured