Recovery of ranavirus dsDNA from formalin-fixed archival material.
The extraction and amplification of nucleic acid from formalin-fixed and paraffin-embedded tissues has become an important exercise in the collection of retrospective epidemiological data. A protocol is described that enables the extraction and amplification of dsDNA from fixed tissues within paraffin blocks and from specimens stored in 10% (aq) formalin. The procedure can be used for the examination of ranavirus DNA within archival tissues thereby providing valuable data for identifying the origin and tracing the spread of ranaviruses.
Kattenbelt
J A
JA
CSIRO, Australian Animal Health Laboratory, Geelong, Victoria, Australia.
Hyatt
A D
AD
Gould
A R
AR
D000818:Animals; D017821:Boidae / Q000821:virology; D004279:DNA, Viral / Q000302:isolation & purification*; D005404:Fixatives; D005557:Formaldehyde; D010232:Paraffin; D010471:Perches / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017995:Ranavirus / Q000235:genetics*
D004279:DNA, Viral; D005404:Fixatives; D005557:Formaldehyde; D010232:Paraffin