Gene-transfer systems for human endothelial cells. stewart.martin@nottingham.ac.uk.
By virtue of its location and importance in a number of pathophysiological processes the endothelium represents an attractive target tissue for gene-transfer and gene-therapy strategies. Although it is important to maximise gene-transfer to endothelial cells in such strategies primary human endothelial cells have proven to be rather intransigent to a variety of transfection techniques both in vitro and in vivo. We report on the variety of techniques in current use, revealing their strengths and weaknesses, indicate the steps that should ideally be taken to optimise expression and discuss the usefulness and future directions for viral mediated transduction.
Martin
S G
SG
University of Nottingham, Laboratory of Molecular Oncology, Cancer Research Campaign Department of Clinical Oncology, City Hospital, Nottingham, UK.
Murray
J C
JC
D004730:Endothelium, Vascular / Q000378:metabolism*; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D006801:Humans; D011401:Promoter Regions, Genetic