title: Purification, crystallization and x-ray analysis of crystals of pectate lyase A from Exwinia chrysanthemi.
abstract: Pectate lyase A is secreted by Erwinia chrysanthemi and is a virulence factor for soft rot diseases in plants. Crystals of pectate lyase A were obtained by vapor-diffusion techniques in the presence of polyethylene glycol. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 48.96, b = 148.86, c = 78.61 A, beta = 97.32 degrees. The crystals contain two protein molecules of 38 kDa per asymmetric unit and diffract to 2.4 A using Cu Kalpha radiation.
authors.affiliation: School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110--2499, USA.
authors.forename: C N
authors.initials: CN
authors.lastname: Doan
authors.forename: M K
authors.initials: MK
authors.lastname: Caughron
authors.forename: J C
authors.initials: JC
authors.lastname: Myers
authors.forename: N W
authors.initials: NW
authors.lastname: Breakfield
authors.forename: R L
authors.initials: RL
authors.lastname: Oliver
authors.forename: M D
authors.initials: MD
authors.lastname: Yoder
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D016974:Dickeya chrysanthemi / Q000201:enzymology*; D008958:Models, Molecular; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase