Retinal stem cells in the adult mammalian eye.
The mature mammalian retina is thought to lack regenerative capacity. Here, we report the identification of a stem cell in the adult mouse eye, which represents a possible substrate for retinal regeneration. Single pigmented ciliary margin cells clonally proliferate in vitro to form sphere colonies of cells that can differentiate into retinal-specific cell types, including rod photoreceptors, bipolar neurons, and Müller glia. Adult retinal stem cells are localized to the pigmented ciliary margin and not to the central and peripheral retinal pigmented epithelium, indicating that these cells may be homologous to those found in the eye germinal zone of other nonmammalian vertebrates.
Tropepe
V
V
Department of Anatomy and Cell Biology, University of Toronto, Medical Sciences Building 1105, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
Coles
B L
BL
Chiasson
B J
BJ
Horsford
D J
DJ
Elia
A J
AJ
McInnes
R R
RR
van der Kooy
D
D
D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D048429:Cell Size; D002470:Cell Survival; D002478:Cells, Cultured; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000096:biosynthesis; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D051379:Mice; D009419:Nerve Tissue Proteins; D064231:Nestin; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000196:embryology; D012160:Retina / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D017948:Retinal Rod Photoreceptor Cells / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis
D018398:Homeodomain Proteins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D014157:Transcription Factors; C088975:Vsx2 protein, mouse; D016222:Fibroblast Growth Factor 2