Rapid discrimination of monkey B virus from human herpes simplex viruses by PCR in the presence of betaine.
A PCR method to amplify DNA segments of the glycoprotein G gene of monkey B virus (BV) was achieved by adding betaine to the PCR mixture, in spite of the high G+C content of this gene. No product was obtained when DNA of human herpes simplex viruses (HSVs) was used as the template under the same conditions. Thus, this PCR method is useful in discriminating BV from HSVs.
Hirano
M
M
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
Nakamura
S
S
Okada
M
M
Ueda
M
M
Mukai
R
R
D000818:Animals; D001622:Betaine; D002522:Chlorocebus aethiops; D005814:Genes, Viral; D006568:Herpesvirus 1, Cercopithecine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013698:Templates, Genetic; D014709:Vero Cells
D001622:Betaine