Effects of glucose and protein sources on bovine embryo development in vitro.
Oviductal factors may be obtained by ultrafiltration of conditioned medium, added to a simple media and used in bovine embryo culture. In this study, we aimed to analyze the development of bovine embryos produced with oviductal factors compared to those cultured in the presence of BSA or serum, the effects of glucose in presence of these protein supplements, and the ability of oviductal factors to support embryo development during the entire culture period. In vitro produced bovine zygotes from slaughterhouse ovaries were cultured in modified-synthetic oviduct fluid (mSOF) alone or supplemented with (1) oviductal factors, (2) BSA and (3) FCS. Oviductal factors showed embryotrophic activity, although with blastocyst rates lower than those in BSA and FCS. Glucose (1.5 mM) added at Day 2 of culture did not affect development in the presence of oviductal factors. The number of cells in expanded blastocysts was unaffected by the presence of glucose or any of the protein supplements used. Both BSA and FCS, respectively, improved blastocyst rates of Day 6 embryos produced with oviductal factors. The effect of oviductal factors was masked by the presence of BSA during the entire culture. FCS promoted an earlier appearance of blastocysts. It is concluded that the effect of glucose on in vitro embryo development depends upon the source of protein. Oviductal factors are not an appropriate supplement for embryos beyond Day 6 of culture in SOF, although blastocyst rates of such embryos may be increased by culturing them in the presence of FCS or BSA.
Gomez
E
E
Centro de Selección y Reproducción Animal, CIATA, Consejería de Agricultura, Camino de los Claveles s/n. SOMIO, 33203, Gijon Asturias, Spain. enriquegp@princast.es
Diez
C
C
D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D002452:Cell Count / Q000662:veterinary; D017077:Culture Media, Conditioned; D005314:Embryonic and Fetal Development; D005187:Fallopian Tubes / Q000737:chemistry / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D005312:Fetal Blood / Q000502:physiology*; D005947:Glucose / Q000502:physiology*; D008297:Male; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D011897:Random Allocation; D012710:Serum Albumin, Bovine / Q000502:physiology*; D014462:Ultrafiltration
D017077:Culture Media, Conditioned; D012710:Serum Albumin, Bovine; D005947:Glucose