Studies on tissue expression of HBV in children with chronic hepatitis type B using Immunomax technique.
The study aimed at employing the Immunomax technique to detect the markers of HBV replication (HBcAg and HBV-DNA) in liver biopsy material, obtained from children with chronic hepatitis type B. In line with the currently modified classification of chronic hepatitis and with the increasing potential of antiviral therapy it seemed purposeful to supplement routine staining techniques with studies at the molecular level. Our studies demonstrated the effective detection of both the core antigen and HBV-DNA in liver tissue in children using immunocytochemical techniques and in situ hybridization, amplified with the Immunomax technique. HBcAg was detected in 26 out of 27 liver biopsies from patients with chronic hepatitis type B and with replication of the virus. HBV-DNA was detected in all study children with HBV infection and in 2 out of 5 cases of chronic hepatitis of a distinct etiology. No significant relationships could be found between the detection of tissue HBV markers on the one hand and the intensity of inflammatory lesions or severity of fibrosis on the other.
Kasprzak
A
A
Department of Histology and Embryology, K. Marcinkowski University of Medical Sciences, Poznań.
Biczysko
W
W
Zabel
M
M
Wysocki
J
J
Surdyk-Zasada
J
J
D001706:Biopsy; D002467:Cell Nucleus / Q000378:metabolism; D002648:Child; D003593:Cytoplasm / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017403:In Situ Hybridization / Q000379:methods; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000821:virology*
D004279:DNA, Viral; D006512:Hepatitis B Core Antigens