Effect of oral micronized purified flavonoid fraction treatment on leukocyte adhesion molecule expression in patients with chronic venous disease: a pilot study.
OBJECTIVE
The purpose of this study was to determine the effects of a micronized purified flavonoid fraction treatment on surface expression of leukocyte adhesion molecules in chronic venous disease (CVD).


METHODS
Twenty patients with chronic venous disease were assessed with the use of clinical and Duplex scanning criteria. Consenting patients were treated for 60 days with a micronized purified flavonoid fraction treatment (500 mg twice daily). Blood was collected from a foot vein immediately before the start of treatment and within 1 week after the treatment was stopped. Neutrophil and monocyte surface adhesion molecule expression was determined by flow cytometry using the monoclonal antibodies to CD11b and CD62L.


RESULTS
Neutrophil CD11b (248:212), monocyte CD11B (204:190), neutrophil CD62L (130:97 [P =.002]), and monocyte CD62L (170:121 [P =.03]) were determined, respectively, before and after treatment. All values are arbitrary units and represent median values.


CONCLUSIONS
Micronized purified flavonoid fraction treatment for 60 days seems to decrease the surface expression of CD62L by neutrophils and by monocytes. The clinical significance of this finding needs to be explored further. It is feasible to use changes in the levels of these molecules as a marker for response to therapy in chronic venous disease.
Shoab
S S
SS
Department of Surgery & Haematology, University College London Medical School, United Kingdom.
Porter
J B
JB
Scurr
J H
JH
Coleridge-Smith
P D
PD
D000284:Administration, Oral; D015815:Cell Adhesion Molecules / Q000187:drug effects / Q000378:metabolism*; D002908:Chronic Disease; D004145:Diosmin / Q000008:administration & dosage*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D019041:L-Selectin / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D013997:Time Factors; D014689:Venous Insufficiency / Q000188:drug therapy* / Q000378:metabolism
D015815:Cell Adhesion Molecules; D019041:L-Selectin; D004145:Diosmin