Dynamics of beta-glucosidase Zm-p60.1 ectopic expression during transgenic pollen development: a histochemical approach.
Zm-p60.1 is maize cDNA coding cytokinin-glucoside specific beta-glucosidase. Indigogenic method was used for histochemical localization of Zm-p60.1 beta-glucosidase activity in various developmental stages of transgenic tobacco anthers. Expression of Zm-p60.1 cDNA in T7 tobacco plants is controlled by the CaMV 35S promoter. Another type of tobacco transformant expresses Zm-p60.1 under the control of LAT 52 promoter. Histochemical detection has proved different patterns of beta-glucosidase activity during tobacco pollen development in these two types of transformants. Zm-p60.1 beta-glucosidase activity had not direct influence on pollen germinability.
Dubová
J
J
Department of Plant Physiology and Anatomy, Faculty of Sciences, Masaryk University, Brno, Czech Republic. dubova@sci.muni.cz
Mlejnek
P
P
Brzobohatý
B
B
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011058:Pollen / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana; D014170:Transformation, Genetic; D003313:Zea mays / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D001617:beta-Glucosidase / Q000235:genetics*
D017931:DNA Primers; D012333:RNA, Messenger; D018749:RNA, Plant; C071257:cytokinin-beta-glucosidase; D001617:beta-Glucosidase