A simple HPLC method using a microbore column for the analysis of doxorubicin.
Doxorubicin is one of the most potent anti-tumor agents generally used in the treatment of bone cancer. A simple and sensitive HPLC method was developed and validated for the assay of doxorubicin. The method used a C18 Luna microbore column (50 x 1 mm) with a fluorescent detector (505 nm Ex. and 550 nm Em.). The mobile phase consisted of water-acetonitrile-acetic acid (80:19:1, v/v/v, pH 3.0) and the flow rate was 0.1 ml min(-1). Daunomycin was used as the internal standard. This isocratic system required a 10-min run-time, giving a detection limit of 0.02 ng (0.035 pmol per injection). Standard curves were linear over the concentration range of 0.01-0.1 microg ml(-1). Relative standard deviations (R.S.D.) for the within-day, day-to-day precision, and the accuracy measurement for the assay were less than 4.0, 3.2, and 4.1%, respectively. This HPLC method was used to study the in vitro release characteristics of doxorubicin from implantable drug delivery system.
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Department of Pharmaceutical and Administrative Sciences, School of Pharmacy and Allied Health Professions, Creighton University, Omaha, NE 68178, USA.
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D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003630:Daunorubicin / Q000032:analysis; D004317:Doxorubicin / Q000032:analysis* / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry; D004343:Drug Implants; D005069:Evaluation Studies as Topic; D005781:Gelatin Sponge, Absorbable / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D004337:Drug Carriers; D004343:Drug Implants; D014867:Water; D004317:Doxorubicin; D019342:Acetic Acid; C032159:acetonitrile; D003630:Daunorubicin