title: Development of tailor-made glycidyl methacrylate-divinyl benzene copolymer for immobilization of D-amino acid oxidase from Aspergillus species strain 020 and its application in the bioconversion of cephalosporin C.
abstract: A tailor-made glycidyl methacrylate-divinyl benzene (GMA-DVB) copolymer PC-3 was evolved by studying the effect of synthesis variables on binding and expression of D-amino acid oxidase (DAAO) from Aspergillus species strain 020. Almost quantitative binding (100%) and a high yield of immobilization per unit of enzyme loaded was achieved. Optimum pH, optimum temperature and K(m)95% was achieved by using 3% (w/v) solution of ceph C, 48 U of DAAO per g of ceph C, keeping dissolved oxygen level above 50%, maintaining the pH between 7.6 and 7.8 and temperature at 24 degrees C. The immobilized DAAO was used for 60 cycles in a stirred tank reactor.
authors.affiliation: Research and Development, Hindustan Antibiotics Limited, Pimpri, Pune, India
authors.initials: SK
authors.lastname: Mujawar
authors.initials: A
authors.lastname: Kotha
authors.initials: CR
authors.lastname: Rajan
authors.initials: S
authors.lastname: Ponrathnam
authors.initials: JG
authors.lastname: Shewale