MsiK-dependent trehalose uptake in Streptomyces reticuli.
During cultivation in the presence of trehalose Streptomyces reticuli expresses an inducible, highly specific trehalose uptake system that is absent in Streptomyces lividans. A palmitated trehalose-binding protein was identified in the cytoplasmic membrane of mycelia, extracted with the detergent Triton X-100 and purified using a trehalose affinity matrix. Immunological studies showed that within S. reticuli the synthesis of the ATP-binding protein MsiK is induced by trehalose. The data suggest that MsiK assists the trehalose ABC transporter, like the previously described ABC transport systems for maltose and cellobiose/cellotriose, respectively.
Schlösser
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FB Biologie/Chemie, Universität Osnabrück, Barbarastrasse 11, 49069, Osnabrück, Germany. schloesser@biologie.uni-osnabrueck.de
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001426:Bacterial Proteins; D001692:Biological Transport; D002250:Carbon Radioisotopes / Q000378:metabolism; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002250:Carbon Radioisotopes; D003470:Culture Media; D008074:Lipoproteins; D014199:Trehalose; D000251:Adenosine Triphosphatases; C096958:MsiK protein, Streptomyces; D005947:Glucose