Polarized sphingolipid transport from the subapical compartment changes during cell polarity development.
The subapical compartment (SAC) plays an important role in the polarized transport of proteins and lipids. In hepatoma-derived HepG2 cells, fluorescent analogues of sphingomyelin and glucosylceramide are sorted in the SAC. Here, evidence is provided that shows that polarity development is regulated by a transient activation of endogenous protein kinase A and involves a transient activation of a specific membrane transport pathway, marked by the trafficking of the labeled sphingomyelin, from the SAC to the apical membrane. This protein kinase A-regulated pathway differs from the apical recycling pathway, which also traverses SAC. After reaching optimal polarity, the direction of the apically activated pathway switches to one in the basolateral direction, without affecting the apical recycling pathway.
van IJzendoorn
S C
SC
Department of Physiological Chemistry, University of Groningen, Groningen, the Netherlands.
Hoekstra
D
D
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity / Q000502:physiology*; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005963:Glucosylceramides / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism*; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D010069:Oxadiazoles / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism; D013449:Sulfonamides
C095088:6-(N-(7-nitro-2,1,3-benzoxadiazol-4-yl)amino)hexanoyl-glucosylceramide; D004791:Enzyme Inhibitors; D005963:Glucosylceramides; D006028:Glycosphingolipids; D007546:Isoquinolines; D010069:Oxadiazoles; D013109:Sphingomyelins; D013449:Sulfonamides; C081708:N-(N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-epsilon-aminohexanoyl)sphingosylphosphorylcholine; D017868:Cyclic AMP-Dependent Protein Kinases; D009327:4-Chloro-7-nitrobenzofurazan; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide