title: Comparison between micellar electrokinetic chromatography and HPLC for the determination of Betamethasone Dipropionate, Clotrimazole and their related substances.
abstract: The complete separation of a composite mixture that consisted of Betamethasone Dipropionate (BMD), Clotrimazole and their derivatives in a pharmaceutical dosage form was achieved within 15 min using sodium dodecyl sulfate (SDS) micellar electrokinetic chromatography (MEKC). For the MEKC separations, electrophoretic media consisting of SDS-phosphate buffer and various concentrations of alcohols or acetonitrile were used. The optimal condition for separating BMD, Clotrimazole and their analogues was found to be 50 mM SDS-15% acetonitrile-5% butanol at pH 7.2. The results demonstrated that the method was valid for the quantitation of BMD, Clotrimazole and analogues with selectivity and precision comparable to that of High-Performance Liquid Chromatography (HPLC).
authors.affiliation: Department of Chemistry, State University of New York at Birmingham, 13902-6000, USA.
authors.forename: M
authors.initials: M
authors.lastname: Lin
authors.forename: N
authors.initials: N
authors.lastname: Wu
mesh_terms: D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000302:isolation & purification; D000935:Antifungal Agents / Q000032:analysis / Q000302:isolation & purification; D001623:Betamethasone / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003022:Clotrimazole / Q000032:analysis* / Q000302:isolation & purification; D005240:Feasibility Studies; D005938:Glucocorticoids; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000893:Anti-Inflammatory Agents; D000935:Antifungal Agents; D005938:Glucocorticoids; C011175:betamethasone-17,21-dipropionate; D001623:Betamethasone; D003022:Clotrimazole