Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida.
The outer membrane protein of Oma87 from Pasteurella multocida A:1 has significant similarity to the D15 protective antigen of Haemophilus influenzae (Ruffolo and Adler, 1996). Four fragments of Oma87 from a P. multocida serotype D strain were cloned into a pGEX expression vector and transformed into E. coli JM105. Western blot analysis revealed that convalescent chicken sera reacted with only GST-F1 fusion protein which contained amino acids 18 through to 130 of Oma87 fused to the GST protein. Vaccination with the GST-F1 protein failed to protect chickens against challenge with a virulent P. multocida serotype A.
Mitchison
M
M
Department of Microbiology, Monash University, Clayton, Vic., Australia.
Wei
L
L
Kwang
J
J
Wilkie
I
I
Adler
B
B
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D002645:Chickens; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005982:Glutathione Transferase / Q000378:metabolism; D016979:Pasteurella multocida / Q000737:chemistry / Q000276:immunology*; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D014611:Vaccination / Q000662:veterinary
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C101027:Oma87 protein, bacteria; D010446:Peptide Fragments; D005982:Glutathione Transferase