Comparison of DNA repair protein expression and activities between human fibroblast cell lines with different radiosensitivities.
In order to investigate the molecular basis of variation in response to ionising radiation (IR) in radiotherapy patients, we have studied the expression of several genes involved in DNA double-strand break repair pathways in fibroblast cell lines. Ten lines were established from skin biopsies of cancer patients with different normal-tissue reactions to IR, and 3 from a control individual. For all 10 test cell lines, the cellular radiosensitivity was also known. Using Western blots we measured, in non-irradiated cells, the basal expression levels of ATM, Rad1 and Hus1, involved in the control of cellular DNA damage checkpoints, together with DNA-PKcs, Ku70, Ku80; XRCC4, ligaseIV and Rad51, involved in radiation- induced DSB repair. We also analysed the in vitro enzymatic activities, under non-irradiated conditions, of the DNA-PK and XRCC4/ligaseIV complexes. The levels of expression of the different proteins were similar in all the cell lines, but the activities of the DNA-PK and XRCC4/ligaseIV complexes showed some differences. These differences did not correlate with either the normal tissue response of the patient in vivo or with cellular radiation sensitivity in vitro. The activity differences of these enzyme complexes, therefore, do not account for the variation of responses seen between patients.
Carlomagno
F
F
CRC Human Cancer Genetics Group, University of Cambridge, Strangeways Research Laboratory, Cambridge, United Kingdom.
Burnet
N G
NG
Turesson
I
I
Nyman
J
J
Peacock
J H
JH
Dunning
A M
AM
Ponder
B A
BA
Jackson
S P
SP
D015153:Blotting, Western; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins; D005347:Fibroblasts; D015870:Gene Expression; D006801:Humans; D008025:Ligases; D009369:Neoplasms / Q000473:pathology / Q000532:radiotherapy; D011494:Protein Kinases; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C101467:XRCC4 protein, human; D011494:Protein Kinases; D008025:Ligases