Green fluorescent protein gene insertion of Sendai Virus infection in nude mice: possibility as an infection tracer.
The green fluorescent protein (GFP) marker from jellyfish Aequorea victoria is considered to have potential use in the study of host-pathogen relationships, by tracing infections in living cells, organs and animals. We compared the pathogenicity of Sendai virus with an inserted GFP gene (GFP-SeV) with that of its wild-type (Wt-SeV) to determine the usefulness of the recombinant virus in long-term infection of BALB/c nude (nu/nu) mice. The results indicated that the presence of GFP in infected cells could be analyzed easily and sensitively. GFP helped in identifying and in understanding the cellular sites of viral replication in vitro and in vivo. However, the GFP insertion into the Wt-SeV genome, led to decreased pathogenicity, altering the in vivo viral kinetics.
Agungpriyono
D R
DR
Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Gakuen Kibuna Dai, Japan.
Yamaguchi
R
R
Uchida
K
K
Tohya
Y
Y
Kato
A
A
Nagai
Y
Y
Asakawa
M
M
Tateyama
S
S
D000818:Animals; D001835:Body Weight; D049452:Green Fluorescent Proteins; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018108:Respirovirus / Q000472:pathogenicity* / Q000502:physiology; D010253:Respirovirus Infections / Q000175:diagnosis* / Q000821:virology; D037401:Scyphozoa; D014162:Transfection; D014779:Virus Replication
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins