title: Immunohistochemical demonstration of choline acetyltransferase of a peripheral type (pChAT) in the enteric nervous system of rats.
abstract: Using a recently developed antiserum against a splice variant (pChAT) of choline acetyltransferase, the enzyme which synthesizes acetylcholine, we carried out an immunohistochemical examination in the digestive canal of rats. Positive staining was exclusively localized to neuronal cells and fibers. Positive somata were distributed widely in the intramural ganglia throughout the digestive tract from the esophagus to the rectum. Double staining indicated that, in the rat, virtually all pChAT immunoreactive somata exhibited histochemical activity for acetylcholinesterase but not for NADPH-diaphorase. In the guinea pig, however, there were a few neurons possessing both pChAT and NADPH-diaphorase. We also found a few neuronal somata which were positive for acetylcholinesterase but not for pChAT. The results suggest that pChAT immunohistochemistry is useful for studying the enteric cholinergic system.
authors.affiliation: Molecular Neuroscience Research Center, Shiga University of Medical Science, Seta, Otsu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: I
authors.initials: I
authors.lastname: Tooyama
authors.forename: O
authors.initials: O
authors.lastname: Yasuhara
authors.forename: Y
authors.initials: Y
authors.lastname: Aimi
authors.forename: H
authors.initials: H
authors.lastname: Kimura
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D004386:Duodenum / Q000294:innervation; D017615:Enteric Nervous System / Q000166:cytology / Q000201:enzymology*; D004947:Esophagus / Q000294:innervation; D005753:Gastric Mucosa / Q000294:innervation; D006168:Guinea Pigs; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000294:innervation; D008297:Male; D009092:Mucous Membrane / Q000201:enzymology; D009197:Myenteric Plexus / Q000166:cytology / Q000201:enzymology; D009412:Nerve Fibers / Q000201:enzymology / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase