Alteration of the expression of the hypocretin (orexin) gene by 2-deoxyglucose in the rat lateral hypothalamic area.
Following an i.p. injection of 2-deoxyglucose (2DG), a nonmetabolizable analogue of glucose known to induce intracellular glucopenia, a progressive decrease in the level of hypocretin (Hcrt)/orexin mRNA was observed in the rat lateral hypothalamus while the melanin-concentrating hormone (MCH) expression in neighbouring neurons remained unaffected. This result together with the previously reported stimulation of Hcrt expression by insulin confirms that Hcrt neurons, but not MCH neurons, are sensitive to glucose availability and suggests that they respond through different mechanisms and/or different pathways to intracellular glucopenia and hypoglycemic conditions.
Bayer
L
L
CNRS ESA 6025, Laboratoire d'Histologie, Faculté de Médecine, Besançon, France.
Colard
C
C
Nguyen
N U
NU
Risold
P Y
PY
Fellmann
D
D
Griffond
B
B
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002352:Carrier Proteins; D003847:Deoxyglucose / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000502:physiology*; D007028:Hypothalamic Hormones / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008543:Melanins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D009479:Neuropeptides; D018377:Neurotransmitter Agents / Q000235:genetics* / Q000378:metabolism; D000068797:Orexins; D010907:Pituitary Hormones / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D001786:Blood Glucose; D002352:Carrier Proteins; D007028:Hypothalamic Hormones; D047908:Intracellular Signaling Peptides and Proteins; D008543:Melanins; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D000068797:Orexins; D010907:Pituitary Hormones; C019463:melanin-concentrating hormone; D003847:Deoxyglucose