title: Cell-cell contact of human T cells with fibroblasts changes lymphocytic mRNA expression: increased mRNA expression of interleukin-17 and interleukin-17 receptor.
abstract: Using random arbitrarily primed-reverse transcribed-PCR and sequence analysis, we investigated changes in lymphocytic molecules after cell-cell contact with fibroblasts. An mRNA species which was upregulated in Jurkat T cells by cell-cell contact with MRHF cells (a human foreskin fibroblast line) was identified as coding for the human interleukin-17 receptor. This finding was confirmed by quantitative RT-PCR for the HUT78 and Jurkat T cell lines, for peripheral blood lymphocytes, and for tonsillar T cells. Furthermore, the interleukin-17 mRNA, coding for a proinflammatory cytokine, was also upregulated in peripheral blood lymphocytes and tonsillar T cells after cell-cell contact with fibroblasts. Supernatants obtained from cell-cell contact-stimulated peripheral blood lymphocytes enhance the production of interleukin-6 and interleukin-8 by fibroblast-like synoviocytes and this effect could be blocked by interleukin-17 antibodies. Changes in the mRNA levels of Jurkat T cells induced by cell-cell contact with adherent cells were also found for M-type pyruvate kinase, for tropomyosin TM30 and for the p54nrb gene product.
authors.affiliation: Institute of Medical Immunology, Martin Luther University Halle-Wittenberg, Strasse der OdF 6, D-06097 Halle, Germany. katja.lapp@medizin.uni-halle.de
authors.forename: K
authors.initials: K
authors.lastname: Thiele
authors.forename: D
authors.initials: D
authors.lastname: Riemann
authors.forename: A
authors.initials: A
authors.lastname: Navarrete Santos
authors.forename: J
authors.initials: J
authors.lastname: Langner
authors.forename: A
authors.initials: A
authors.lastname: Kehlen
mesh_terms: D002450:Cell Communication / Q000276:immunology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017931:DNA Primers; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D015850:Interleukin-6 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D019169:Jurkat Cells; D014066:Palatine Tonsil / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D018123:Receptors, Interleukin / Q000235:genetics*; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; C109985:IL17RA protein, human; D020381:Interleukin-17; D015850:Interleukin-6; D016209:Interleukin-8; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins