Anti-inflammatory action of a novel peptide, SEK-1005, isolated from a Streptomyces.
The pharmacological activity of a novel cyclic peptide (SEK-1005: C(45)H(70)N(8)0(13)) isolated from Streptomyces nobilis was studied in rats during the development of inflammation. SEK-1005 (0.1-0.5 mg/kg, i.p.) suppressed the passive Arthus reaction and the carrageenin-induced oedema. A steroidal anti-inflammatory drug, prednisolone (10 mg/kg, i.p.), also was effective on both inflammations. However, indomethacin (5 mg/kg, i.p.), a cyclooxygenase inhibitor, was less effective on the passive Arthus reaction. Also interesting was that the SEK-1005 effect showed its maximum level after a 24-h lag period and that its effect, as well as the prednisolone effect, was reduced by the treatment with a protein synthesis inhibitor, cycloheximide. SEK-1005 and prednisolone also showed marked protection against the adjuvant-induced arthritis, but failed to prevent the tuberculin response. These findings indicate that SEK-1005 is a new type of non-steroidal anti-inflammatory agent with an action similar to that of prednisolone.
Kuriyama
K
K
Medical Research Laboratory, Sekisui Chemical, 2-1 Hyakuyama Shimamoto-cho, Mishima-gun, Osaka, Japan.
Fujiwara
A
A
Inagaki
K
K
Abe
Y
Y
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000517:prevention & control; D001183:Arthus Reaction / Q000517:prevention & control; D002351:Carrageenan; D003513:Cycloheximide / Q000494:pharmacology; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D006968:Hypersensitivity, Delayed / Q000517:prevention & control; D008297:Male; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D013302:Streptomyces / Q000737:chemistry*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010456:Peptides, Cyclic; D011500:Protein Synthesis Inhibitors; C407521:SEK 1005; D002351:Carrageenan; D003513:Cycloheximide