title: Synthesis, biological activity, and molecular modeling of ribose-modified deoxyadenosine bisphosphate analogues as P2Y(1) receptor ligands.
abstract: The structure-activity relationships of adenosine-3', 5'-bisphosphates as P2Y(1) receptor antagonists have been explored, revealing the potency-enhancing effects of the N(6)-methyl group and the ability to substitute the ribose moiety (Nandanan et al. J. Med. Chem. 1999, 42, 1625-1638). We have introduced constrained carbocyclic rings (to explore the role of sugar puckering), non-glycosyl bonds to the adenine moiety, and a phosphate group shift. The biological activity of each analogue at P2Y(1) receptors was characterized by measuring its capacity to stimulate phospholipase C in turkey erythrocyte membranes (agonist effect) and to inhibit its stimulation elicited by 30 nM 2-methylthioadenosine-5'-diphosphate (antagonist effect). Addition of the N(6)-methyl group in several cases converted pure agonists to antagonists. A carbocyclic N(6)-methyl-2'-deoxyadenosine bisphosphate analogue was a pure P2Y(1) receptor antagonist and equipotent to the ribose analogue (MRS 2179). In the series of ring-constrained methanocarba derivatives where a fused cyclopropane moiety constrained the pseudosugar ring of the nucleoside to either a Northern (N) or Southern (S) conformation, as defined in the pseudorotational cycle, the 6-NH(2) (N)-analogue was a pure agonist of EC(50) 155 nM and 86-fold more potent than the corresponding (S)-isomer. The 2-chloro-N(6)-methyl-(N)-methanocarba analogue was an antagonist of IC(50) 51.6 nM. Thus, the ribose ring (N)-conformation appeared to be favored in recognition at P2Y(1) receptors. A cyclobutyl analogue was an antagonist with IC(50) of 805 nM, while morpholine ring-containing analogues were nearly inactive. Anhydrohexitol ring-modified bisphosphate derivatives displayed micromolar potency as agonists (6-NH(2)) or antagonists (N(6)-methyl). A molecular model of the energy-minimized structures of the potent antagonists suggested that the two phosphate groups may occupy common regions. The (N)- and (S)-methanocarba agonist analogues were docked into the putative binding site of the previously reported P2Y(1) receptor model.
authors.affiliation: Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0810, USA.
authors.forename: E
authors.initials: E
authors.lastname: Nandanan
authors.forename: S Y
authors.initials: SY
authors.lastname: Jang
authors.forename: S
authors.initials: S
authors.lastname: Moro
authors.forename: H O
authors.initials: HO
authors.lastname: Kim
authors.forename: M A
authors.initials: MA
authors.lastname: Siddiqui
authors.forename: P
authors.initials: P
authors.lastname: Russ
authors.forename: V E
authors.initials: VE
authors.lastname: Marquez
authors.forename: R
authors.initials: R
authors.lastname: Busson
authors.forename: P
authors.initials: P
authors.lastname: Herdewijn
authors.forename: T K
authors.initials: TK
authors.lastname: Harden
authors.forename: J L
authors.initials: JL
authors.lastname: Boyer
authors.forename: K A
authors.initials: KA
authors.lastname: Jacobson
mesh_terms: D000818:Animals; D003838:Deoxyadenine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D004789:Enzyme Activation; D004910:Erythrocyte Membrane / Q000201:enzymology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D058910:Purinergic P2 Receptor Agonists; D058919:Purinergic P2 Receptor Antagonists; D058472:Receptors, Purinergic P2Y1; D012266:Ribose / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014422:Turkeys; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D003838:Deoxyadenine Nucleotides; D007295:Inositol Phosphates; D008024:Ligands; D058910:Purinergic P2 Receptor Agonists; D058919:Purinergic P2 Receptor Antagonists; D058472:Receptors, Purinergic P2Y1; D012266:Ribose; D010738:Type C Phospholipases