title: Inhaled particle-bound sulfate: effects on pulmonary inflammatory responses and alveolar macrophage function.
abstract: Acid sulfate-coated solid particles are a significant environmental hazard produced primarily by the combustion of fossil fuels. We have previously described a system for the nascent generation of carbonaceous particles surface coated with approximately 140 microg/m(3) acid sulfate [cpSO(4)(2-); 10 mg/m(3) carbon black (CB) and 10 ppm sulfur dioxide (SO(2)) at 85% relative humidity (RH)]. The effects of inhaled cpSO(4)(2-) on pulmonary host defenses are assessed in the present work. Mice were acutely exposed (4 h) to either 10 mg/m(3) CB, 10 ppm SO(2), or their combination at 10% or 85% RH in a nose-only inhalation chamber. No evidence of an inflammatory response was found following any of the exposures as assessed by total cell counts and differential cell counts from bronchoalveolar lavage fluid. However, alveolar macrophage Fc receptor-mediated phagocytosis decreased only following exposure to 140 microg cpSO(4)(2-), significant suppression occurred after 24 h, maximal suppression occurred at 3 days postexposure, and recovery to preexposure levels required 7-14 days. Intrapulmonary bactericidal activity (IBA) was also suppressed only after exposure to 140 microg cpSO(4)(2-); suppression was maximal at 1 day postexposure and recovered by day 7. To assess the effects of lower cpSO(4)(2-) concentrations, mice were repeatedly exposed to 1 mg/m(3) CB and 1 ppm SO(2) at 85% RH ( approximately 20 microg/m(3) cpSO(4)(2-) for 4 h/day) for up to 6 days. A significant decrement in IBA was observed following 5 and 6 days of exposure. These studies indicated that acute or repeated exposure to cpSO(4)(2-) could alter pulmonary host defense mechanisms.
authors.affiliation: Department of Environmental Health, Harvard School of Public Health, Room 2-223, 665 Huntington Avenue, Boston, MA 02115, USA. rwclarke@hsph.harvard.edu
authors.forename: R W
authors.initials: RW
authors.lastname: Clarke
authors.forename: J M
authors.initials: JM
authors.lastname: Antonini
authors.forename: D R
authors.initials: DR
authors.lastname: Hemenway
authors.forename: R
authors.initials: R
authors.lastname: Frank
authors.forename: S R
authors.initials: SR
authors.lastname: Kleeberger
authors.forename: G J
authors.initials: GJ
authors.lastname: Jakab
mesh_terms: D000280:Administration, Inhalation; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002244:Carbon / Q000008:administration & dosage / Q000633:toxicity*; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D018613:Microscopy, Confocal; D010585:Phagocyte Bactericidal Dysfunction / Q000139:chemically induced / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects*; D013458:Sulfur Dioxide / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D013458:Sulfur Dioxide; D002244:Carbon