title: Titanium dioxide ceramics control the differentiated phenotype of cardiac muscle cells in culture.
abstract: A new approach, the cultivation of heart muscle cells on biocompatible scaffolds made from titanium dioxide ceramics was established to provide a mechanism for in vitro engineering of a vital heart tissue. Terminally differentiated ventricular myocytes isolated from hearts of adult rats were kept in primary culture for long periods of time and used as an experimental model. The microenvironmental properties of titanium dioxide ceramics helped to maintain the tissue-like structural organisation of the cardiac cells in vitro. Coating of the cell substrata with fine-grained titanium dioxide ceramics imitating cell surface topography favoured the formation of focal adhesion complexes in the ventral plasma membrane of cardiomyocytes. It also promoted the cellular expression of vinculin, a protein that connects the ECM integrin receptors to the network of cytoplasmic filaments, which define cell shape. This topographical reinforcement of cell-material interactions led to stabilisation of the molecular linkage between the extracellular contacts and the intracellular cytoskeleton and thus assisted the preservation and maintenance of the heart muscle cell differentiated phenotype in long-term primary culture. The results of this work demonstrate a promising pathway for the regulation of cellular organisation in vitro by local geometric control.
authors.affiliation: Institute for Cell Biology, ETH-Hoenggerberg, Zurich, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Polonchuk
authors.forename: J
authors.initials: J
authors.lastname: Elbel
authors.forename: L
authors.initials: L
authors.lastname: Eckert
authors.forename: J
authors.initials: J
authors.lastname: Blum
authors.forename: E
authors.initials: E
authors.lastname: Wintermantel
authors.forename: H M
authors.initials: HM
authors.lastname: Eppenberger
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics / Q000494:pharmacology*; D005260:Female; D005782:Gels; D006321:Heart / Q000187:drug effects*; D006352:Heart Ventricles; D007091:Image Processing, Computer-Assisted; D008422:Materials Testing; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009124:Muscle Proteins / Q000096:biosynthesis; D009206:Myocardium / Q000166:cytology*; D010641:Phenotype; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D016596:Vinculin / Q000096:biosynthesis
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D009124:Muscle Proteins; D016596:Vinculin; C009495:titanium dioxide; D014025:Titanium