Surprisingly little polymorphism in the merozoite-surface-protein-2 (MSP-2) gene of Indian Plasmodium falciparum.
The polymorphism in the merozoite-surface-protein-2 (MSP-2) gene of six Indian Plasmodium falciparum isolates was studied by PCR amplification, cloning and sequencing. One of the isolates showed a deletion of 63 bp and all showed point mutations, although some of these mutations were silent. All the isolates also exhibited 5' and 3' conserved regions, with the two 32-mer amino-acid repeats characteristic of the FC27 family, and none belonged to the IC-1/3D7 family. Although the MSP-2 genes of these isolates represent new allelic sequences, they belong to the FC27 family and show remarkably little variation.
Bhattacharya
P R
PR
Malaria Research Centre, Delhi, India.
Kumar
M
M
Das
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D017125:Genes, Protozoan / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D017780:Malaria Vaccines / Q000235:genetics; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000517:prevention & control; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics*
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D017780:Malaria Vaccines; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium