Preserved solid lipid nanoparticles (SLN) at low concentrations do cause neither direct nor indirect cytotoxic effects in peritoneal macrophages.
In order to investigate the interaction of preserved solid lipid nanoparticles (SLN) with murine peritoneal macrophages (Mpsi), cytotoxicity and proinflammatory effects of two different solid lipid nanoparticles (SLN) preparations consisting of either compritol (CO) or cetyl palmitate (CP) preserved with thiomersal were analyzed. Concentration-dependent cytotoxic effects were observed using the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Secretion of interleukin-6 by Mpsi following incubation with CO and CP SLN did not differ from secretion by untreated cells; proinflammatory cytokines interleukin-12 and tumor-necrosis-factor-alpha as further indicators of immunomodulatory effects were not detectable. These findings paralleled our previous findings that unpreserved CO and CP SLN did not induce immunomodulatory effects but cytotoxicity at higher concentrations. There were no synergistic cytotoxic effects of preservative and SLN. Thus, preservation of SLN using thiomersal does not appear to cause increased cytotoxicity and immunomodulatory effects following incubation with Mpsi.
Schöler
N
N
Department of Medical Microbiology, Benjamin Franklin Hospital, Free University of Berlin, Hindenburgdamm 27, D-12203, Berlin, Germany.
Zimmermann
E
E
Katzfey
U
U
Hahn
H
H
Müller
R H
RH
Liesenfeld
O
O
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D004339:Drug Compounding; D004797:Enzyme-Linked Immunosorbent Assay; D005079:Excipients / Q000008:administration & dosage / Q000737:chemistry; D005227:Fatty Acids / Q000008:administration & dosage / Q000737:chemistry; D005260:Female; D018664:Interleukin-12 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008055:Lipids / Q000008:administration & dosage* / Q000737:chemistry; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010168:Palmitates / Q000008:administration & dosage / Q000737:chemistry; D010316:Particle Size; D020442:Poloxamer / Q000008:administration & dosage / Q000737:chemistry; D011310:Preservatives, Pharmaceutical / Q000737:chemistry / Q000633:toxicity*; D013849:Thimerosal / Q000008:administration & dosage / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D017077:Culture Media, Conditioned; D004337:Drug Carriers; D005079:Excipients; D005227:Fatty Acids; D015850:Interleukin-6; D008055:Lipids; D010168:Palmitates; D011310:Preservatives, Pharmaceutical; D014409:Tumor Necrosis Factor-alpha; D020442:Poloxamer; C086409:glyceryl behenate; D018664:Interleukin-12; D013849:Thimerosal; C006957:cetyl palmitate