Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage.
The changes in viability, susceptibility to peroxidation and fatty acid composition of total phospholipid were studied in boar spermatozoa during 5 day liquid storage in a standard or alpha-tocopherol (alphaT) enriched diluent. The sperm rich fraction of the ejaculates was collected from 6-month old boars. Sperm viability progressively decreased during storage and alphaT inclusion into the diluent significantly inhibited this trend. alphaT inclusion also decreased significantly peroxidation (TBARS production of spermatozoa). Spermatozoa stored in the treatment diluent became rapidly enriched in alphaT with a concomitant decrease of alphaT content in the medium. The proportion of polyunsaturates, mainly 22:6n-3, decreased with a complementary increase in the content of the saturates, mainly 18:0. The inclusion of alphaT into the diluent was effective in totally preventing the significant decrease of 22:6n-3 observed in sperm phospholipid in the control samples during the storage period. It is concluded that the alphaT inclusion in the boar semen diluent increased cell viability through its prevention of an oxidative reduction in the levels of the major polyunsaturated fatty acids, namely 22:6n-3.
Cerolini
S
S
Istituto di. Zootecnica Veterinaria, via Celoria 10, 20133, Milan, Italy. silvia.cerolini@unimi.it
Maldjian
A
A
Surai
P
P
Noble
R
R
D000818:Animals; D002849:Chromatography, Gas / Q000662:veterinary; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D002855:Chromatography, Thin Layer / Q000662:veterinary; D004396:Coloring Agents / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009930:Organic Chemicals; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry*; D011419:Propidium / Q000737:chemistry; D012661:Semen / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D012662:Semen Preservation / Q000662:veterinary*; D013094:Spermatozoa / Q000502:physiology; D013552:Swine / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry; D013997:Time Factors; D014810:Vitamin E / Q000737:chemistry*
D004396:Coloring Agents; D005227:Fatty Acids; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D010743:Phospholipids; C093207:SYBR-14; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D011419:Propidium