Repeatability and reproducibility of retention data and band profiles on reversed-phase liquid chromatography columns. IV. Results obtained with Luna C18 (2) columns.
The reproducibility of retention data and band profile characteristics was investigated for a series of columns packed with Luna C18 (2), a silica-based, reversed-phase adsorbent. High precision data were obtained and statistically compared among five columns from the same batch (column-to-column reproducibility) and nine columns from as many different batches (batch-to-batch reproducibility). These data were acquired under five different sets of chromatographic conditions, for a group of 30 neutral, acidic, and basic compounds selected as probes following an experimental protocol previously described. This work is part of a study on the precision of chromatographic analyses. Its purpose is to illustrate the contribution of the stationary phase reproducibility to this precision.
Kele
M
M
Department of Chemistry, University of Tennessee, Knoxville 37996-1600, USA.
Guiochon
G
G
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000592:standards; D015203:Reproducibility of Results