title: Hydrogen peroxide induces LFA-1-dependent neutrophil adherence to cardiac myocytes.
abstract: Adult cardiac myocytes express intercellular adhesion molecule (ICAM)-1 in response to cytokine stimulation. This allows stable adhesion of chemotactically stimulated but not unstimulated neutrophils. In the current study, we demonstrated that brief exposure of ICAM-1-expressing cardiac myocytes to H(2)O(2) promoted transient adhesive interactions between myocytes and neutrophils without added chemotactic factors. This transient adhesion differed in two ways from the stable adhesion promoted by exogenous chemotactic factors. It occurred more rapidly, peaking within 15 min, and it was dependent on leukocyte function-associated antigen (LFA)-1 (CD11a/CD18) on the neutrophil interacting with ICAM-1 on the myocyte. In contrast, chemotactic factor-induced adhesion peaked at 60 min and was dependent on Mac-1 (CD11b/CD18). The transient adhesion could be completely inhibited by platelet-activating factor (PAF)-receptor antagonists WEB-2086 and SDZ-64-412. These results indicate that canine neutrophils may utilize both LFA-1 and Mac-1 to adhere to adult cardiac myocytes, with LFA-1 triggered by a PAF-like activity induced in myocytes by H(2)O(2).
authors.affiliation: Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: H
authors.initials: H
authors.lastname: Lu
authors.forename: K
authors.initials: K
authors.lastname: Youker
authors.forename: C
authors.initials: C
authors.lastname: Ballantyne
authors.forename: M
authors.initials: M
authors.lastname: Entman
authors.forename: C W
authors.initials: CW
authors.lastname: Smith
mesh_terms: D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002630:Chemotactic Factors / Q000494:pharmacology; D004285:Dogs; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007700:Kinetics; D016169:Lymphocyte Function-Associated Antigen-1 / Q000494:pharmacology*; D016177:Macrophage-1 Antigen / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D009504:Neutrophils / Q000502:physiology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D001381:Azepines; D002630:Chemotactic Factors; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D010975:Platelet Aggregation Inhibitors; D014230:Triazoles; C052518:WEB 2086; D018799:Intercellular Adhesion Molecule-1; D006861:Hydrogen Peroxide