Identification of genes regulated by UV/salicylic acid.
OBJECTIVE
Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments described here, differential-display RT-PCR was used to identify those cDNAs that are differentially modulated by UV radiation with and without the addition of salicylic acid.


METHODS
Differential-display RT-PCR was used to identify differentially expressed genes.


RESULTS
Eight such cDNAs are presented: lactate dehydrogenase (LDH-beta), nuclear encoded mitochondrial NADH ubiquinone reductase 24 kDa (NDUFV2), elongation initiation factor 4B (eIF4B), nuclear dots protein SP100, nuclear encoded mitochondrial ATPase inhibitor (IF1), a cDNA similar to a subunit of yeast CCAAT transcription factor HAP5, and two expressed sequence tags (AA187906 and AA513156).


CONCLUSIONS
Sequences of four of these genes contained NF-kappaB DNA binding sites of the type that may attract transrepressor p55/p55 NF-kappaB homodimers. Down-regulation of these genes upon UV irradiation may contribute to increased cell survival via suppression of p53 independent apoptosis.
Paunesku
T
T
Bioscience Division, Argonne National Laboratory, IL 60439, USA.
Chang-Liu
C M
CM
Shearin-Jones
P
P
Watson
C
C
Milton
J
J
Oryhon
J
J
Salbego
D
D
Milosavljevic
A
A
Woloschak
G E
GE
D000251:Adenosine Triphosphatases / Q000235:genetics; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects*; D016158:Genes, p53 / Q000502:physiology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D016328:NF-kappa B / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D020156:Salicylic Acid / Q000494:pharmacology*; D014466:Ultraviolet Rays
D016328:NF-kappa B; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase; D000251:Adenosine Triphosphatases; D020156:Salicylic Acid; D007213:Indomethacin