Development of a novel biomarker of free radical damage in reperfusion injury after cardiac arrest.
In a porcine model of cardiopulmonary resuscitation (CPR), we investigated changes in the plasma levels of 8-iso-PGF(2alpha), a marker for oxidative injury, and 15-keto-dihydro-PGF(2alpha), an inflammatory response indicator during the post-resuscitation period after cardiac arrest. Twelve piglets were subjected to either 2 or 5 min (VF2 and VF5 group) of ventricular fibrillation (VF) followed by 5 min of closed-chest CPR. Six piglets without cardiac arrest were used as controls. In VF5 group, 8-iso-PGF(2alpha) in the jugular bulb plasma (draining the brain) increased four-fold. Jugular bulb 8-iso-PGF(2alpha) in the control group remained unchanged. The 15-keto-dihydro-PGF(2alpha) also increased four-fold in the VF5 group. Thus, 8-iso-PGF(2alpha) and 15-keto-dihydro-PGF(2alpha) measurements in jugular bulb plasma may be used as biomarkers for quantification of free radical catalyzed oxidative brain injury and inflammatory response in reperfusion injury.
Basu
S
S
Department of Geriatrics, Faculty of Medicine, Uppsala University, P. O. Box 609, SE-751 25, Uppsala, Sweden. samar.basu@geriatrik.uu.se
Nozari
A
A
Liu
X L
XL
Rubertsson
S
S
Wiklund
L
L
D000818:Animals; D015415:Biomarkers; D016887:Cardiopulmonary Resuscitation; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D004195:Disease Models, Animal; D028441:F2-Isoprostanes; D005260:Female; D005609:Free Radicals; D006323:Heart Arrest / Q000097:blood* / Q000150:complications; D007601:Jugular Veins; D008297:Male; D015394:Molecular Structure; D015428:Myocardial Reperfusion Injury / Q000097:blood*; D013552:Swine; D014693:Ventricular Fibrillation
D015415:Biomarkers; D028441:F2-Isoprostanes; D005609:Free Radicals; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost