Expression of growth hormone receptor in the human brain.
This study was designed to investigate the presence of growth hormone receptor (GHR) expression in the human brain tissue, both normal and tumoral, as well as in the human glioblastoma cell line U87MG. Reverse transcription-polymerase chain reaction revealed the presence of GHR mRNA in all brain samples investigated and in U87MG cells. GHR immunoreactivity was also detected in this cell line using both immunocytochemistry and western blotting. All together, our data demonstrate the existence of GHR expression within the central nervous system (CNS), thus supporting a possible role for GH in the CNS physiology.
Castro
J R
JR
Departamento de Fisioloxía, Facultad de Medicina, San Francisco 1, 15705 Santiago de, Compostela, Spain.
Costoya
J A
JA
Gallego
R
R
Prieto
A
A
Arce
V M
VM
Señarís
R
R
D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism*; D005625:Frontal Lobe / Q000378:metabolism; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D010296:Parietal Lobe / Q000378:metabolism; D011986:Receptors, Somatotropin / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013702:Temporal Lobe / Q000378:metabolism; D014407:Tumor Cells, Cultured
D011986:Receptors, Somatotropin