Characterization, chromosomal assignment, and tissue expression of a novel human gene belonging to the ARF GAP family.
We have identified and characterized a novel human ADP-ribosylation factor GTPase-activating protein (ARFGAP1) gene that is related to other members of the ARF GAP family. The full-length cDNA for human ARFGAP1 was cloned following the identification of an EST obtained by large-scale cDNA library sequencing through a Blast search of public databases. Structurally, ARFGAP1 encodes a polypeptide of 516 amino acids, which contained a typical GATA-1-type zinc finger motif (CXXCX(16)CXXC) with the four cysteine residues that are highly conserved among other members of the ARF GAP family. The conserved ARF GAP domain may emphasize the biological importance of this gene. The ARFGAP1 gene, which contained 16 exons ranging from 0.5 to 9.3 kb, was mapped to human chromosome 22q13.2-q13.3 using radiation hybridization and in silico analyses. ARFGAP1 is strongly expressed in endocrine glands and testis. Interestingly, the expression of ARFGAP1 in testis is about sixfold higher than that in ovary, indicating a possible role of ARFGAP1 in the physiological function of sperm. Expression of ARFGAP1 in four human fetal tissues and seven cancer cell lines was also detected.
Zhang
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C
Department of Genomics and Proteomics, Beijing Institute of Radiation Medicine, Chinese National Human Genome Center at Beijing, 27 Taiping Road, Beijing, 100850, People's Republic of China.
Yu
Y
Y
Zhang
S
S
Liu
M
M
Xing
G
G
Wei
H
H
Bi
J
J
Liu
X
X
Zhou
G
G
Dong
C
C
Hu
Z
Z
Zhang
Y
Y
Luo
L
L
Wu
C
C
Zhao
S
S
He
F
F
D020727:ADP-Ribosylation Factors / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004702:Endocrine Glands / Q000378:metabolism; D005091:Exons / Q000235:genetics; D020224:Expressed Sequence Tags; D005326:Fetal Proteins / Q000096:biosynthesis / Q000235:genetics; D020690:GTPase-Activating Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D008168:Lung / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009928:Organ Specificity; D013737:Testis / Q000378:metabolism; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers / Q000235:genetics*
C503237:ARFGAP1 protein, human; C503240:ARFGAP3 protein, human; D018076:DNA, Complementary; D005326:Fetal Proteins; D020690:GTPase-Activating Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D020727:ADP-Ribosylation Factors