title: Salmonella pathogenicity island 2-dependent evasion of the phagocyte NADPH oxidase.
abstract: A type III protein secretion system encoded by Salmonella pathogenicity island 2 (SPI2) has been found to be required for virulence and survival within macrophages. Here, SPI2 was shown to allow Salmonella typhimurium to avoid NADPH oxidase-dependent killing by macrophages. The ability of SPI2-mutant bacteria to survive in macrophages and to cause lethal infection in mice was restored by abrogation of the NADPH oxidase-dependent respiratory burst. Ultrastructural and immunofluorescence microscopy demonstrated efficient localization of the NADPH oxidase in the proximity of vacuoles containing SPI2-mutant but not wild-type bacteria, suggesting that SPI2 interferes with trafficking of oxidase-containing vesicles to the phagosome.
authors.affiliation: Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
authors.forename: A
authors.initials: A
authors.lastname: Vazquez-Torres
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Jones-Carson
authors.forename: D W
authors.initials: DW
authors.lastname: Holden
authors.forename: S M
authors.initials: SM
authors.lastname: Lucia
authors.forename: M C
authors.initials: MC
authors.lastname: Dinauer
authors.forename: P
authors.initials: P
authors.lastname: Mastroeni
authors.forename: F C
authors.initials: FC
authors.lastname: Fang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002563:Cerium / Q000032:analysis; D005798:Genes, Bacterial; D006878:Hydroxides; D017737:Macrophages, Peritoneal / Q000201:enzymology* / Q000382:microbiology* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D019255:NADPH Oxidases / Q000378:metabolism*; D010545:Peroxides / Q000032:analysis; D010588:Phagosomes / Q000382:microbiology; D016897:Respiratory Burst; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014617:Vacuoles / Q000201:enzymology / Q000382:microbiology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D006878:Hydroxides; D010545:Peroxides; D013481:Superoxides; D002563:Cerium; C048873:cerium hydroxide; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate