The integration of canine genetic maps with the canine karyotype using specific gene amplification of chromosome-specific DNA.
We have used a rapid approach to place markers that are already represented in current genetic maps onto individual chromosomes in species for which chromosome paints exist. PCR-based techniques are used to look for the presence of individual marker genes within each chromosome-specific DNA pool. The presence of a given marker within a DNA pool allows assignment of the complete radiation hybrid group, or linkage group from which the marker is drawn, to an individual chromosome. We have used this method with a new set of canine chromosome paints (Yang et al., 1999). In this way, we have assigned 39 of 44 published RH or syntenic RH groups to canine chromosomes, together with 33 of 40 canine linkage groups in a recently published map (Neff et al., 1999).
Sargan
D R
DR
Department of Clinical Veterinary Medicine, University of Cambridge, UK.
Yang
F
F
Milne
B
B
O'Brien
P C
PC
Solanky
N
N
Squire
M
M
Rens
W
W
Ferguson-Smith
M A
MA
D000818:Animals; D002874:Chromosome Mapping; D020223:Chromosome Painting; D004247:DNA / Q000032:analysis; D004285:Dogs / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D007621:Karyotyping; D016133:Polymerase Chain Reaction; D011831:Radiation Genetics
D005819:Genetic Markers; D004247:DNA