T(84) epithelial cells respond to 5-hydroxytryptamine when grown in serum-free media.
The aim of this study was to establish the cause of insensitivity of T(84) human colonic epithelial cells to 5-hydroxytryptamine (5-HT). Monolayers of T(84) cells were placed in modified Ussing chambers for measurement of short-circuit current, an index of secretion. When grown in serum-supplemented media, T(84) cells gave secretory responses to acetylcholine and forskolin but not to 5-HT. When grown in AIM V serum-free media, T(84) cells responded to 5-HT. Chromatographic analysis with fluorimetric detection showed high levels of 5-HT (1.8 microM) in the serum. This contamination is probably responsible for subsequent desensitization of T(84) cells to 5-HT.
Burleigh
D
D
Molecular Pharmacology Section, Division of Biomedical Sciences, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, University of London, Mile End Road, London, UK.
Fernandes
K
K
Perrett
D
D
D000109:Acetylcholine / Q000494:pharmacology; D002460:Cell Line; D003106:Colon / Q000166:cytology* / Q000187:drug effects; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells / Q000187:drug effects*; D005470:Fluorometry; D006801:Humans; D012701:Serotonin / Q000032:analysis / Q000494:pharmacology*
D016895:Culture Media, Serum-Free; D012701:Serotonin; D000109:Acetylcholine