Creating cataracts of varying hardness to practice extracapsular cataract extraction and phacoemulsification(2).
We describe a method to harden the nucleus of crystalline lenses in postmortem human globes by intralenticular injection of a mixture of paraformaldehyde and glutaraldehyde (Karnovsky's solution). Evaluation of the nuclear manipulations, using the Miyake-Apple posterior video/photographic technique, shows that the method provides suitable specimens for practicing extracapsular cataract extraction and phacoemulsification.
Pandey
S K
SK
Center for Research on Ocular Therapeutics and Biodevices, Storm Eye Institute, Medical University of South Carolina, Charleston, South Carolina 29425-5536, USA.
Werner
L
L
Escobar-Gomez
M
M
Visessook
N
N
Peng
Q
Q
Apple
D J
DJ
D002386:Cataract / Q000139:chemically induced* / Q000473:pathology; D002387:Cataract Extraction / Q000379:methods*; D004338:Drug Combinations; D004493:Education / Q000379:methods; D005557:Formaldehyde / Q000494:pharmacology*; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007267:Injections; D007908:Lens, Crystalline / Q000187:drug effects* / Q000473:pathology; D009885:Ophthalmology / Q000193:education*; D018918:Phacoemulsification; D011108:Polymers / Q000494:pharmacology*
D004338:Drug Combinations; D011108:Polymers; D005557:Formaldehyde; D005976:Glutaral; C003043:paraform