Non-linear membrane properties of sacral sphincter motoneurones in the decerebrate cat.
1. Responses to pudendal afferent stimulation and depolarizing intracellular current injection were examined in sacral sphincter motoneurones in decerebrate cats. 2. In 16 animals examined, 2-10 s trains of electrical stimulation of pudendal afferents evoked sustained sphincter motoneurone activity lasting from 5 to >50 s after stimulation. The sustained response was observed in: 11 animals in the absence of any drugs; two animals after the intravenous administration of 5-hydroxytryptophan (5-HTP; <= 20 mg kg-1); one animal in which methoxamine was perfused onto the ventral surface of the exposed spinal cord; and two animals following the administration of intravenous noradrenergic agonists. 3. Extracellular and intracellular recordings from sphincter motoneurones revealed that the persistent firing evoked by afferent stimulation could be terminated by motoneurone membrane hyperpolarization during micturition or by intracellular current injection. 4. Intracellular recordings revealed that 22/40 sphincter motoneurones examined displayed a non-linear, steep increase in the membrane potential in response to depolarizing ramp current injection. The mean voltage threshold for this non-linear membrane response was -43 +/- 3 mV. Five of the 22 cells displaying the non-linear membrane response were recorded prior to the administration of 5-HTP; 17 after the intravenous administration of 5-HTP (<= 20 mg kg-1). 5. It is concluded that sphincter motoneurones have a voltage-sensitive, non-linear membrane response to depolarization that could contribute to sustained sphincter motoneurone firing during continence.
Paroschy
K L
KL
Department of Physiology, Faculty of Medicine, University of Manitoba, 730 William Avenue, Winnipeg, Canada R3E 3J7.
Shefchyk
S J
SJ
D006916:5-Hydroxytryptophan / Q000494:pharmacology; D001003:Anal Canal / Q000294:innervation*; D000818:Animals; D002415:Cats; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D003655:Decerebrate State / Q000503:physiopathology*; D004558:Electric Stimulation; D004594:Electrophysiology; D005260:Female; D007275:Injections, Intravenous; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D009046:Motor Neurons / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology; D017711:Nonlinear Dynamics; D012445:Sacrococcygeal Region; D013116:Spinal Cord / Q000503:physiopathology*; D014521:Urethra / Q000294:innervation*; D014554:Urination / Q000502:physiology
D006916:5-Hydroxytryptophan