Molecular and immunological analyses of the Borrelia turicatae Bdr protein family.
Here, we describe the molecular and immunological characterization of the bdr gene family of Borrelia turicatae, a relapsing-fever spirochete. Nine bdr alleles belonging to two different subfamilies were sequenced and localized to linear plasmids. Anti-Bdr antiserum was generated and used to analyze Bdr expression in pre- and postinfection isogenic populations. The analyses presented here provide a detailed characterization of the Bdr proteins in a relapsing-fever spirochete species, enhancing our understanding of these proteins at the genus-wide level.
Carlyon
J A
JA
Department of Microbiology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298, USA.
Roberts
D M
DM
Theisen
M
M
Sadler
C
C
Marconi
R T
RT
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D001898:Borrelia / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D019143:Evolution, Molecular; D015723:Gene Library; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D010957:Plasmids; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D001426:Bacterial Proteins; D015345:Oligonucleotide Probes