In vitro expression of proalpha1(I) collagen mRNA by human pre-osteoclastic cells.
In vitro studies have demonstrated that the extracellular matrix modulates the cell phenotype. In the present study we have investigated in vitro proalpha1(I) collagen mRNA expression in a human pre-osteoclastic cell line (FLG 29.1 cells) in basal condition and after various stimuli. In addition, in order to evaluate the effect of cell-cell interactions on collagen type I mRNA expression, we have cultured the human pre-osteoclastic cells FLG 29.1 with either the human osteoblast-like cell line Saos-2 or the bovine bone endothelial cell line BBE. We showed that the FLG 29.1 cells express proal (I) collagen mRNA, whose expression is modulated by phorbol esters (TPA). Co-culturing FLG 29.1 cells with either Saos-2 or BBE cells induced decrease of proalpha1(I) collagen mRNA expression.
Masi
L
L
Department of Clinical Physiopathology, University of Firenze, Italy.
Malentacchi
C
C
Benvenuti
S
S
Amedei
A
A
Bigozzi
U
U
Montali
E
E
Brandi
M L
ML
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D002450:Cell Communication; D018920:Coculture Techniques; D004727:Endothelium / Q000378:metabolism; D000091344:Filaggrin Proteins; D015870:Gene Expression; D006801:Humans; D007948:Leukemia, Monocytic, Acute; D010006:Osteoblasts / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism*; D011347:Procollagen / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D014407:Tumor Cells, Cultured
C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D011347:Procollagen; D012333:RNA, Messenger