Epitope mapping of the immunodominant invariable region of Borrelia burgdorferi VlsE in three host species.
VlsE, the variable surface antigen of Borrelia burgdorferi, contains a 26-amino-acid-long immunodominant invariable region, IR(6). In the present study, three overlapping 14-mer peptides reproducing the sequence of IR(6) were used as peptide-based enzyme-linked immunosorbent assay antigens to map this invariable region in infected monkeys, mice, and human Lyme disease patients. Antibodies of the two primate species appeared to recognize IR(6) as a single antigenic determinant, while mouse antibodies recognized multiple epitopes within this region.
Liang
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Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, USA.
Philipp
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D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D001426:Bacterial Proteins; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes; D008074:Lipoproteins / Q000276:immunology*; D008193:Lyme Disease / Q000276:immunology; D020852:Lyme Neuroborreliosis / Q000276:immunology; D008253:Macaca mulatta; D051379:Mice; D008809:Mice, Inbred C3H; D008957:Models, Genetic; D008969:Molecular Sequence Data; D013045:Species Specificity
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; D016056:Immunodominant Epitopes; D008074:Lipoproteins; C105546:VlsE protein, Borrelia burgdorferi