title: Functional and morphologic changes of the ileal mucosa after ileoanal pouch procedure.
abstract: BACKGROUND
  Restorative proctocolectomy is used widely for treatment of ulcerative colitis and familial polyposis coli. Limited information is available regarding the morphologic and functional adaptation of the mucosa in a functioning ileoanal pouch.
  
  
  METHODS
  Ileal pouch specimens from patients who underwent pouch reconstruction (mean 7.5 years postcolectomy, n = 12) were compared with normal ileum (n = 15) and normal colon (n = 5). Amino-oligopeptidase (AOP) and maltase activity were measured as parameters of normal ileal function. Histologic samples were examined for the presence of neutrophils and plasma cells, the villus to crypt height ratio, and the degree of crypt hyperplasia, villus blunting, and goblet cell mass. Data were analyzed by analysis of variance.
  
  
  RESULTS
  The AOP activity in the normal ileum was 73 +/- 32 units of enzymatic activity per gram of mucosal protein; the AOP activities of the pouch and colon were 21 +/- 22 and 16 +/- 10, respectively. The maltase activity of the normal ileum measured 254 +/- 116 units of enzymatic activity per gram of mucosal protein, and the maltase activities of the pouch and colon were 57 +/- 71 units and 29 +/- 25 units, respectively. The ileal pouch mucosa demonstrated little acute inflammation and varying degrees of chronic inflammation. Morphologically, the ileal pouch mucosa demonstrated a range of adaptations, including villus blunting and crypt hyperplasia. Several specimens contained immature epithelial cells.
  
  
  CONCLUSIONS
  The AOP and maltase activities in mucosa from ileoanal pouches and colon were significantly lower than those in normal ileal mucosa. Ileoanal pouch mucosa from humans undergoes adaptive changes to resemble colonic mucosa both morphologically and functionally.
authors.affiliation: Division of Pediatric Surgery, UCLA School of Medicine, Los Angeles, CA 90095-1749, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Kawaguchi
authors.forename: J C
authors.initials: JC
authors.lastname: Dunn
authors.forename: M S
authors.initials: MS
authors.lastname: Saing
authors.forename: G
authors.initials: G
authors.lastname: Cortina
authors.forename: E W
authors.initials: EW
authors.lastname: Fonkalsrud
mesh_terms: D000328:Adult; D018826:CD13 Antigens / Q000378:metabolism; D005260:Female; D006801:Humans; D007082:Ileum / Q000201:enzymology / Q000473:pathology*; D007249:Inflammation / Q000473:pathology; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D011184:Postoperative Period; D016737:Proctocolectomy, Restorative; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000520:alpha-Glucosidases; D018826:CD13 Antigens