Quantitation of nifedipine in human plasma by on-line solid-phase extraction and high-performance liquid chromatography.
An analytical methodology for nifedipine quantitation in plasma by on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) is described. The SPE cartridges contain C2 and the analytes nifedipine and nitrendipine (internal standard) are separated on a C18 column with a mobile phase consisting of acetonitrile-13 mM phosphate buffer pH 7 (65:35, v/v) followed by UV detection at 338 nm. Validation of the method demonstrated good recoveries (>90%), sensitivity (limit of quantification, 2 ng/ml), based on a 500 microl sample volume, accuracy and precision (<5.5% in concentrations greater than the limit of quantitation). This methodology has been used for bioequivalence studies.
Yriti
M
M
Analysis Laboratory, Research Institute, Sant Pau Hospital, Barcelona, Spain. ifarm@santpau.es
Parra
P
P
Iglesias
E
E
Barbanoj
J M
JM
D002121:Calcium Channel Blockers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009543:Nifedipine / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002121:Calcium Channel Blockers; D009543:Nifedipine