Effect of atypical antipsychotics on phencyclidine-induced expression of arc in rat brain.
The effect of atypical antipsychotics on the immediate-early gene, arc (activity-regulated cytoskeleton-associated gene), expression was investigated in phencyclidine (PCP)-treated rats using RT-PCR. Administration of PCP (10 mg/kg) increased arc mRNA levels in the prefrontal cortex, nucleus accumbens and posterior cingulate cortex. Pretreatment with clozapine (20 mg/kg), olanzapine (10 mg/kg) and risperidone (2 mg/kg), but not haloperidol (2 mg/kg), prevented PCP-induced arc expression in the prefrontal cortex and nucleus accumbens. Pretreatment of haloperidol increased the striatal arc mRNA levels. Clozapine, olanzapine and haloperidol inhibited the PCP-induced arc expression in the posterior cingulate cortex. These results suggest that the effects of antipsychotic drugs on PCP-induced arc expression in the prefrontal cortex and nucleus accumbens are useful for distinguishing atypical antipsychotic properties of the drugs.
Nakahara
T
T
Department of Chemistry, Faculty of Science, Kyushu University, Ropponmatasu, Fukuoka, Japan.
Kuroki
T
T
Hashimoto
K
K
Hondo
H
H
Tsutsumi
T
T
Motomura
K
K
Ueki
H
H
Hirano
M
M
Uchimura
H
H
D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D001569:Benzodiazepines; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D003024:Clozapine / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D006220:Haloperidol / Q000494:pharmacology; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D000077152:Olanzapine; D010622:Phencyclidine / Q000494:pharmacology*; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018967:Risperidone / Q000494:pharmacology; D014018:Tissue Distribution
D014150:Antipsychotic Agents; D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C092069:activity regulated cytoskeletal-associated protein; D001569:Benzodiazepines; D010890:Pirenzepine; D010622:Phencyclidine; D003024:Clozapine; D006220:Haloperidol; D018967:Risperidone; D000077152:Olanzapine