Advantage of gene gun-mediated over intramuscular inoculation of plasmid DNA vaccine in reproducible induction of specific immune responses.
Utilizing a plasmid DNA encoding a single cytotoxic T lymphocyte (CTL) epitope and that encoding ovalbumin (OVA), we compared the reproducibility in the induction of immune responses by gene gun and intramuscular immunization. As compared to intramuscular inoculation, gene gun DNA immunization appeared to bring about highly reproducible and reliable results in the induction of specific CTL and IFN-gamma production to the CTL epitope and production of anti-OVA IgG. The results obtained by intramuscular inoculation vary significantly. Our data shown here strongly suggest that gene gun immunization of skin is a much more reliable method for DNA vaccination to induce effective immune responses in an animal model.
Yoshida
A
A
Department of Microbiology and Immunology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, Japan.
Nagata
T
T
Uchijima
M
M
Higashi
T
T
Koide
Y
Y
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D019470:Biolistics; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003603:Cytotoxins / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D007371:Interferon-gamma / Q000378:metabolism; D008089:Listeria monocytogenes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D010957:Plasmids / Q000008:administration & dosage* / Q000276:immunology; D015203:Reproducibility of Results; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D000942:Antigens, Bacterial; D003603:Cytotoxins; D000939:Epitopes; D007074:Immunoglobulin G; D010446:Peptide Fragments; D019444:Vaccines, DNA; D007371:Interferon-gamma; D010047:Ovalbumin