The reactions of myoglobin, normal adult hemoglobin, sickle cell hemoglobin and hemin with hydroxyurea.
The kinetics of the reaction of hydroxyurea (HU) with myoglobin (Mb), hemin, sickle cell hemoglobin (HbS), and normal adult hemoglobin (HbA) were determined using optical absorption spectroscopy as a function of time, wavelength, and temperature. Each reaction appeared to follow pseudo-first order kinetics. Electron paramagnetic resonance spectroscopy (EPR) experiments indicated that each reaction produced an FeNO product. Reactions of hemin and the ferric forms of HbA, HbS, and myoglobin with HU also formed the NO adduct. The formation of methemoglobin and nitric oxide-hemoglobin from these reactions may provide further insight into the mechanism of how HU benefits sickle cell patients.
Rupon
J W
JW
Department of Chemistry, Wake Forest University, Winston-Salem, NC 27103, USA.
Domingo
S R
SR
Smith
S V
SV
Gummadi
B K
BK
Shields
H
H
Ballas
S K
SK
King
S B
SB
Kim-Shapiro
D B
DB
D000328:Adult; D000818:Animals; D003545:Cysteine / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D006427:Hemin / Q000737:chemistry*; D006441:Hemoglobin A / Q000737:chemistry*; D006451:Hemoglobin, Sickle / Q000737:chemistry*; D006736:Horses; D006801:Humans; D006918:Hydroxyurea / Q000737:chemistry* / Q000494:pharmacology; D007700:Kinetics; D009211:Myoglobin / Q000737:chemistry*; D010108:Oxyhemoglobins / Q000737:chemistry; D011487:Protein Conformation; D013053:Spectrophotometry
D006451:Hemoglobin, Sickle; D009211:Myoglobin; D010108:Oxyhemoglobins; C017595:oxymyoglobin; C029775:oxyhemoglobin S; D006427:Hemin; D006441:Hemoglobin A; C029774:oxyhemoglobin A; D003545:Cysteine; D006918:Hydroxyurea