title: A novel heparan sulphate with high degree of N-sulphation and high heparin cofactor-II activity from the brine shrimp Artemia franciscana.
abstract: With the aid of heparinase and heparitinases from Flavobacterium heparinum and 13C and IH NMR spectroscopy it was shown that the heparan sulphate isolated from the brine shrimp Artemia franciscana exhibits structural features intermediate between those of mammalian heparins and heparan sulphates. These include an unusually high degree of N-sulphation (with corresponding very low degree of N-acetylation), a relatively high content of iduronic acid residues (both unsulphated and 2-O-sulphated) and a relatively low degree of 6-O-sulphation of the glucosamine residues. The major sequences (glucuronic acid-->N-sulphated glucosamine and glucuronic acid-->N, 6-disulphated glucosamine) are most probably arranged in blocks. Although exhibiting negligible anticlotting activity in the APTT and anti-factor Xa assays the A. franciscana heparan sulphate has a high heparin cofactor-II activity (about 1/3 that of heparin).
authors.affiliation: Departamento de Bioquimica, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
authors.forename: S F
authors.initials: SF
authors.lastname: Chavante
authors.forename: E A
authors.initials: EA
authors.lastname: Santos
authors.forename: F W
authors.initials: FW
authors.lastname: Oliveira
authors.forename: M
authors.initials: M
authors.lastname: Guerrini
authors.forename: G
authors.initials: G
authors.lastname: Torri
authors.forename: B
authors.initials: B
authors.lastname: Casu
authors.forename: C P
authors.initials: CP
authors.lastname: Dietrich
authors.forename: H B
authors.initials: HB
authors.lastname: Nader
mesh_terms: D000818:Animals; D001156:Artemia / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D002417:Cattle; D004587:Electrophoresis, Agar Gel; D015844:Heparin Cofactor II / Q000378:metabolism*; D006497:Heparan Sulfate / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D014574:Uronic Acids / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D015844:Heparin Cofactor II; D006497:Heparan Sulfate; D014574:Uronic Acids