Purification, crystallization and preliminary x-ray diffraction analysis of carboxyhaemoglobin-II from the fish Piaractus mesopotamicus (pacu).
Carboxyhaemoglobin-II isolated from the pacu (Piaractus mesopotamicus) has been crystallized and X-ray diffraction data were collected to 2.0 A resolution using synchrotron radiation. Crystals were characterized as belonging to the space group I23; preliminary structural analysis reveals the presence of one dimer in the asymmetric unit.
Fadel
V
V
Departamento de Física, IBILCE-UNESP, CP 136, CEP 15054-000, São José do Rio Preto SP, Brazil.
Honda
R T
RT
Dellamano
M
M
Smarra
A L
AL
Delatorre
P
P
Olivieri
J R
JR
Bonilla-Rodriguez
G O
GO
de Azevedo
W F
WF
D000818:Animals; D002263:Carboxyhemoglobin / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D003531:Cypriniformes / Q000097:blood*; D008958:Models, Molecular; D011487:Protein Conformation
D002263:Carboxyhemoglobin