Fetal porcine dopaminergic cell survival in vitro and its relationship to embryonic age.
One of the critical factors in the survival of embryonic neural grafts is the age at which the population of donor neurons is harvested. This is especially the case for the developing dopaminergic neurons in the embryonic ventral mesencephalon, which are used for neural grafts in Parkinson's disease (PD). The donor age for optimal harvesting of these cells has been well characterized in the mouse, rat, and marmoset, and to a lesser extent in humans. However, the best donor age for porcine ventral mesencephalic tissue has not been ascertained, even though the use of this tissue for xenografts has been explored both experimentally and clinically. In this study the effect of donor age on dopaminergic cell survival was assessed in vitro, from a range of fetal pigs aged from E24 to E35. The number of tyrosine hydroxylase (TH)-positive cells per ventral mesencephalon was then calculated after 1 and 7 days in culture. E26-E27 embryos gave the highest yield of such cells at both survival time points, suggesting that this will be the optimal age for harvesting tissues whether for experimental or clinical nigral xenograft programs.
Barker
R A
RA
MRC Cambridge Centre for Brain Repair, UK. rab46@cus.cam.ac.uk
Ratcliffe
E
E
Richards
A
A
Dunnett
S B
SB
D000818:Animals; D016380:Brain Tissue Transplantation; D002470:Cell Survival / Q000502:physiology*; D004298:Dopamine / Q000378:metabolism*; D016332:Fetal Tissue Transplantation; D005865:Gestational Age; D006801:Humans; D066298:In Vitro Techniques; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation*; D051381:Rats; D013552:Swine; D014183:Transplantation, Heterologous; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine