title: A novel gibberellin-induced gene from rice and its potential regulatory role in stem growth.
abstract: Os-GRF1 (Oryza sativa-GROWTH-REGULATING FACTOR1) was identified in a search for genes that are differentially expressed in the intercalary meristem of deepwater rice (Oryza sativa L.) internodes in response to gibberellin (GA). Os-GRF1 displays general features of transcription factors, contains a functional nuclear localization signal, and has three regions with similarities to sequences in the database. One of these regions is similar to a protein interaction domain of SWI2/SNF2, which is a subunit of a chromatin-remodeling complex in yeast. The two other domains are novel and found only in plant proteins of unknown function. To study its role in plant growth, Os-GRF1 was expressed in Arabidopsis. Stem elongation of transformed plants was severely inhibited, and normal growth could not be recovered by the application of GA. Our results indicate that Os-GRF1 belongs to a novel class of plant proteins and may play a regulatory role in GA-induced stem elongation.
authors.affiliation: Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312, USA.
authors.forename: E
authors.initials: E
authors.lastname: van der Knaap
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Kende
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant; D005875:Gibberellins / Q000494:pharmacology*; D018519:Meristem / Q000187:drug effects / Q000254:growth & development; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; C091848:GRF1 protein, Zea mays; D005875:Gibberellins; D010940:Plant Proteins