title: Molecular characterization of Brucella strains isolated from marine mammals.
abstract: Recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as Brucella species by conventional phenotypic analysis. This study found the 16S rRNA gene from one representative isolate was identical to the homologous sequences of Brucella abortus, B. melitensis, B. canis, and B. suis. IS711-based DNA fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical Brucella species. In general, fingerprint patterns grouped by host species. The data suggest that the marine mammal isolates are distinct types of Brucella and not one of the classical species or biovars invading new host species. In keeping with historical precedent, the designation of several new Brucella species may be appropriate.
authors.affiliation: National Animal Disease Center, Agricultural Research Service, USDA, Ames, Iowa 50010, USA. bbricker@nadc.ars.usda.gov
authors.forename: B J
authors.initials: BJ
authors.lastname: Bricker
authors.forename: D R
authors.initials: DR
authors.lastname: Ewalt
authors.forename: A P
authors.initials: AP
authors.lastname: MacMillan
authors.forename: G
authors.initials: G
authors.lastname: Foster
authors.forename: S
authors.initials: S
authors.lastname: Brew
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002002:Brucella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002003:Brucella abortus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017347:Brucella melitensis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D004275:DNA, Ribosomal / Q000235:genetics; D004285:Dogs; D004289:Dolphins / Q000382:microbiology*; D006041:Goats; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012053:Reindeer; D012377:Rodentia; D046048:Seals, Earless / Q000382:microbiology*; D012756:Sheep; D013552:Swine
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S