Diesel exhaust (DE)-induced cytokine expression in human bronchial epithelial cells: a study with a new cell exposure system to freshly generated DE in vitro.
We devised a new in vitro cell exposure system to freshly generated diesel exhaust (DE), different from conventional in vitro culture systems, to examine the effects of DE on human epithelial cells. Using this system, we investigated the effects of DE on cytokine gene expressions in BET-1A human bronchial epithelial cells. DE significantly decreased [(3)H]thymidine incorporation into BET-1A cells. DE had a significant stimulatory effect on interleukin (IL)-8 release to a marked degree. IL-8 and transforming growth factor (TGF)-beta1 messenger RNA (mRNA) expression were induced by DE in a time-dependent manner. The gas obtained by filtration of DE alone did not show a sustained increase in IL-8 protein levels and showed no induction of IL-8 mRNA, suggesting that DE particles (DEPs) play an important role in the induction of IL-8 at both mRNA and protein levels. Antioxidants, pyrrolidine dithiocarbamate, and N-acetyl-L-cysteine significantly inhibited IL-8 mRNA and protein levels by BET-1A cells. These results indicate that freshly generated DEPs may be important in the induction of cytokines such as IL-8 and TGF-beta1 relevant to allergic airway inflammation.
Abe
S
S
Fourth Department of Internal Medicine, Nippon Medical School, Japan.
Takizawa
H
H
Sugawara
I
I
Kudoh
S
S
D000111:Acetylcysteine / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology*; D002461:Cell Line, Transformed; D004847:Epithelial Cells / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D011759:Pyrrolidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D013859:Thiocarbamates / Q000494:pharmacology; D013936:Thymidine / Q000493:pharmacokinetics; D016212:Transforming Growth Factor beta / Q000032:analysis; D014316:Tritium; D001335:Vehicle Emissions / Q000009:adverse effects*
D000975:Antioxidants; D016166:Free Radical Scavengers; D016209:Interleukin-8; D011759:Pyrrolidines; D012333:RNA, Messenger; D013859:Thiocarbamates; D016212:Transforming Growth Factor beta; D001335:Vehicle Emissions; D014316:Tritium; C020972:pyrrolidine dithiocarbamic acid; D013936:Thymidine; D000111:Acetylcysteine