Molecular cloning and characterization of a cDNA for Glycyrrhiza glabra cycloartenol synthase.
A cDNA clone (GgCAS1) encoding cycloartenol synthase (CAS) has been isolated from Glycyrrhiza glabra (licorice) by cross-hybridization with that of Pisum sativum CAS as a probe. The deduced amino acid sequence of GgCAS1 exhibits 89%, 83% and 81% identity to those of Pisum sativum, Panax ginseng and Arabidopsis thaliana CASs, respectively. CAS activity has been detected in the homogenate of the yeast transformed with the expression vector containing the open reading frame of GgCAS1. Southern blot analysis suggested that at least two CAS genes exist in the licorice genome. In Northern blot analysis, the strong signal for CAS mRNA is detected in the cultured licorice cells of all growth phases, but no significant increase of CAS mRNA expression was observed in the cells treated with the 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor, pravastatin.
Hayashi
H
H
Niigata College of Pharmacy, Japan.
Hiraoka
N
N
Ikeshiro
Y
Y
Kushiro
T
T
Morita
M
M
Shibuya
M
M
Ebizuka
Y
Y
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000235:genetics*; D018744:DNA, Plant / Q000096:biosynthesis / Q000235:genetics; D015723:Gene Library; D006035:Glycyrrhiza / Q000201:enzymology* / Q000235:genetics*; D017403:In Situ Hybridization; D019751:Intramolecular Transferases / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D005894:Panax / Q000235:genetics; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D019751:Intramolecular Transferases; C021923:cycloartenol synthase