Expression and regulation of CNTF receptor-alpha in the in situ and in oculo grafted adult rat adrenal medulla.
Cultured and transplanted adrenal medullary cells respond to ciliary neurotrophic factor (CNTF) with neurite formation and improved cell survival although the presence of the CNTF receptor-alpha (CNTFRalpha) has been unclear. This study show that CNTFRalpha mRNA was expressed in the postnatal day 1 as well as in the adult rat adrenal medulla. The highest CNTFRalpha mRNA signal was found in the ganglion cells of the adrenal medulla. After transplantation of adrenal medullary tissue the CNTFRalpha mRNA levels were down-regulated in the chromaffin cells. CNTF treatment of grafts did not normalize the receptor levels, but treatment with nerve growth factor (NGF) did. Thus, we demonstrate that CNTFRalpha mRNA is expressed in adrenal medulla, the levels becomes down-regulated after transplantation, but normalized after treatment with NGF.
Förander
P
P
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Brené
S
S
Strömberg
I
I
D000313:Adrenal Medulla / Q000166:cytology / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D019439:Chromaffin Cells / Q000378:metabolism / Q000637:transplantation*; D005123:Eye; D005260:Female; D017403:In Situ Hybridization; D020932:Nerve Growth Factor / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020801:Receptor, Ciliary Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values; D016042:Transplantation, Heterotopic
D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D020932:Nerve Growth Factor