title: Effects of parenteral nutrition supplemented with glutamine or glutamine dipeptides on liver antioxidant and detoxication systems in rats.
abstract: Our aim was to determine the effects of glutamine or alanyl glutamine parenteral supplementation on the liver oxidant/antioxidant balance and on cytochrome-P450-mediated detoxication in rats. Animals were infused for 5 d with standard total parenteral nutrition (TPN), glutamine-enriched TPN, or alanyl glutamine-enriched TPN. The hepatic concentration of glutathione was reduced, and the levels of thiobarbituric-acid-reactive substances (TBARS) were increased in animals receiving standard TPN. Both glutamine and alanyl glutamine supplementation normalized glutathione, but thiobarbituric-acid-reactive substance concentration was only decreased by ananyl glutamine. This effect was parallel to a partial recovery of the activity of antioxidant enzymes. Cytochrome-P450 liver content, cytochrome-P450-dependent monooxygenases, and antipyrine clearance were not modified by glutamine or alanyl glutamine. Our data suggest a better protection against free radicals by alanyl glutamine supplementation and an absence of effects of both glutamine and alanyl glutamine on liver oxidative metabolism.
authors.affiliation: Hospital of León, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Matilla
authors.forename: J
authors.initials: J
authors.lastname: Ortíz
authors.forename: P
authors.initials: P
authors.lastname: González
authors.forename: F
authors.initials: F
authors.lastname: García-Díez
authors.forename: F
authors.initials: F
authors.lastname: Jorquera
authors.forename: J M
authors.initials: JM
authors.lastname: Culebras
authors.forename: J
authors.initials: J
authors.lastname: González-Gallego
authors.forename: M J
authors.initials: MJ
authors.lastname: Tuñón
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004151:Dipeptides / Q000008:administration & dosage*; D005973:Glutamine / Q000008:administration & dosage*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008658:Inactivation, Metabolic; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism*; D008297:Male; D010289:Parenteral Nutrition, Total; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004151:Dipeptides; D017392:Thiobarbituric Acid Reactive Substances; D005973:Glutamine; D003577:Cytochrome P-450 Enzyme System; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; C054122:alanylglutamine