title: Characterization of the interactions between the glycine transporters GLYT1 and GLYT2 and the SNARE protein syntaxin 1A.
abstract: In this study we have examined the effect of the SNARE protein syntaxin 1A on the glycine transporters GLYT1 and GLYT2. Our results demonstrate a functional and physical interaction between both glycine transporters and syntaxin 1A. Co-transfection of syntaxin 1A with GLYT1 or GLYT2 in COS cells resulted in approximately 40% inhibition in glycine transport. This inhibition was reversed by the syntaxin 1A-binding protein, Munc18. Furthermore, immunoprecipitation studies showed a physical interaction between syntaxin 1A and both transporters in COS cells and in rat brain tissue. Finally, we conclude that this physical interaction resulted in a partial removal of the glycine transporters from the plasma membrane as demonstrated by biotinylation studies.
authors.affiliation: Centro de Biología Molecular 'Severo Ochoa', Facultad de Ciencias, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Geerlings
authors.forename: B
authors.initials: B
authors.lastname: López-Corcuera
authors.forename: C
authors.initials: C
authors.lastname: Aragón
mesh_terms: D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D050487:Glycine Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D000954:Antigens, Surface; D002352:Carrier Proteins; D050487:Glycine Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C493269:Slc6a5 protein, rat; C493266:Slc6a9 protein, rat; C494210:Snap25 protein, rat; C494213:Stx1a protein, rat; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1