WECHE: a novel hematopoietic regulatory factor.
Previously, we described AGM-derived endothelial cell lines that either inhibited or permitted the development of erythroid or B cells. We utilized a differential gene expression method to isolate a chemokine, termed WECHE, from one of these cell lines. WECHE inhibited the formation of erythroid cells but had no effect on either myeloid or B cell formation. WECHE repressed BFU-E development from either mouse fetal liver or bone marrow progenitor cells but had no effect on colony formation induced by IL-3 or IL-7. WECHE reduced HPP-CFC production from fetal liver-derived stem cells. WECHE hindered the growth of yolk sac-derived endothelial cells. WECHE was also chemotactic for bone marrow cells. Thus, WECHE is a novel chemokine that regulates hematopoietic differentiation.
Ohneda
O
O
Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan. oohneda@kaiju.medic.kumamoto-u.ac.jp
Ohneda
K
K
Nomiyama
H
H
Zheng
Z
Z
Gold
S A
SA
Arai
F
F
Miyamoto
T
T
Taillon
B E
BE
McIndoe
R A
RA
Shimkets
R A
RA
Lewin
D A
DA
Suda
T
T
Lasky
L A
LA
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D018925:Chemokines / Q000235:genetics / Q000502:physiology*; D019743:Chemokines, CXC; D002633:Chemotaxis / Q000502:physiology; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D018925:Chemokines; D019743:Chemokines, CXC; C118893:Cxcl15 protein, mouse