Purification and characterization of a catalase from the facultatively psychrophilic bacterium Vibrio rumoiensis S-1(T) exhibiting high catalase activity.
Catalase from the facultatively psychrophilic bacterium Vibrio rumoiensis S-1(T), which was isolated from an environment exposed to H(2)O(2) and exhibited high catalase activity, was purified and characterized, and its localization in the cell was determined. Its molecular mass was 230 kDa, and the molecule consisted of four identical subunits. The enzyme, which was not apparently reduced by dithionite, showed a Soret peak at 406 nm in a resting state. The catalytic activity was 527,500 U. mg of protein(-1) under standard reaction conditions at 40 degrees C, 1.5 and 4.3 times faster, respectively, than those of the Micrococcus luteus and bovine catalases examined under the same reaction conditions, and showed a broad optimum pH range (pH 6 to 10). The catalase from strain S-1(T) is located not only in the cytoplasmic space but also in the periplasmic space. There is little difference in the activation energy for the activity between strain S-1(T) catalase and M. luteus and bovine liver catalases. The thermoinstability of the activity of the former catalase were significantly higher than those of the latter catalases. The thermoinstability suggests that the catalase from strain S-1(T) should be categorized as a psychrophilic enzyme. Although the catalase from strain S-1(T) is classified as a mammal type catalase, it exhibits the unique enzymatic properties of high intensity of enzymatic activity and thermoinstability. The results obtained suggest that these unique properties of the enzyme are in accordance with the environmental conditions under which the microorganism lives.
Yumoto
I
I
Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan. yumoto@hniri.go.jp
Ichihashi
D
D
Iwata
H
H
Istokovics
A
A
Ichise
N
N
Matsuyama
H
H
Okuyama
H
H
Kawasaki
K
K
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D002384:Catalysis / Q000187:drug effects; D002417:Cattle; D003593:Cytoplasm / Q000201:enzymology; D004227:Dithionite / Q000378:metabolism; D004795:Enzyme Stability; D006418:Heme / Q000032:analysis; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight; D019897:Periplasm / Q000201:enzymology; D013057:Spectrum Analysis; D013696:Temperature; D014733:Vibrio / Q000166:cytology / Q000201:enzymology*
D000596:Amino Acids; D004227:Dithionite; D006418:Heme; D006861:Hydrogen Peroxide; D002374:Catalase