title: A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains.
abstract: Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM16 and HVT strains.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Xing
authors.forename: Q
authors.initials: Q
authors.lastname: Xie
authors.forename: R W
authors.initials: RW
authors.lastname: Morgan
authors.forename: K A
authors.initials: KA
authors.lastname: Schat
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D002642:Chick Embryo; D019084:Fluorescent Antibody Technique, Indirect; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000276:immunology*; D006825:Hybridomas; D007114:Immunization; D008214:Lymphocytes / Q000166:cytology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012703:Serotyping; D015534:Trans-Activators / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D014162:Transfection; D014764:Viral Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D015534:Trans-Activators; D014764:Viral Proteins; C075882:ICP4 protein, Gallid herpesvirus 2