Cryptic polyadenylation sites within the coding sequence of three yeast genes expressed in tobacco.
Three yeast genes, MIP (mitochondrial DNA polymerase) and two genes, YCF1 (yeast cadmium factor 1) and PDR5 (pleiotropic drug resistance 5), conferring multidrug resistance, were provided with the cauliflower mosaic virus 35S transcription promoter and introduced into tobacco using an Agrobacterium tumefaciens T-DNA-derived vector. Transcripts of each gene much shorter than those expected were found in the transgenic plants. RT-PCR and S1 nuclease mapping of the PDR5 and MIP transcripts demonstrated the presence of one (PDR5), or several close (MIP), cryptic polyadenylation site(s) within the coding sequence of these yeast genes. Possible sequences involved in polyadenylation are discussed.
Grec
S
S
Unité de Biochimie Physiologique, Université Catholique de Louvain, Louvain la Neuve, Belgium.
Wang
Y
Y
Le Guen
L
L
Negrouk
V
V
Boutry
M
M
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D004256:DNA Polymerase I / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D005800:Genes, Fungal / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics; D010947:Plants, Toxic; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic
D018528:ATP-Binding Cassette Transporters; D005656:Fungal Proteins; D008565:Membrane Proteins; C092438:PDR5 protein, S cerevisiae; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C089179:YCF1 protein, S cerevisiae; D011061:Poly A; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase; C068352:MIP1 protein, S cerevisiae