title: A novel viral alpha2,3-sialyltransferase (v-ST3Gal I): transfer of sialic acid to fucosylated acceptors.
abstract: The substrate specificity of an alpha2,3-sialyltransferase (v-ST3Gal I) obtained from myxoma virus infected RK13 cells has been determined. Like mammalian sialyltransferase enzymes, the viral enzyme contains the characteristic L- and S-sialyl motif sequences in its catalytic domain. Analysis of the deduced amino acid sequences of cloned sialyltransferases suggests that v-ST3Gal I is closely related to mammalian ST3Gal IV. v-ST3Gal I catalyzes the transfer of sialic acid from CMP-NeuAc to Type I (Galbeta1-3GlcNAcbeta) II (Galbeta1-4GlcNAcbeta) and III (Galbeta1-3GalNAcbeta) acceptors. In addition, the viral enzyme also transfers sialic acid to the fucosylated acceptors Lewis(x) and Lewis(a). This substrate specificity is unlike any sialyltransferases described to date, though it is most comparable with those of mammalian ST3Gal IV enzymes. The products from reactions with fucosylated acceptors were characterized by capillary zone electrophoresis, (1)H-NMR spectroscopy and mass spectrometry. They were shown to be 2,3-sialylated Lewis(x) and 2,3-sialylated Lewis(a), respectively.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada,
authors.forename: K
authors.initials: K
authors.lastname: Sujino
authors.forename: R J
authors.initials: RJ
authors.lastname: Jackson
authors.forename: N W
authors.initials: NW
authors.lastname: Chan
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
authors.forename: M M
authors.initials: MM
authors.lastname: Palcic
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002522:Chlorocebus aethiops; D019075:Electrophoresis, Capillary; D008969:Molecular Sequence Data; D009233:Myxoma virus / Q000201:enzymology*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D010802:Phylogeny; D011817:Rabbits; D012799:Sialyltransferases / Q000145:classification / Q000378:metabolism*; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase