Protective effect of the antioxidant stobadine against cyclophosphamide and irradiation induced oxidative stress.
The antioxidant stobadine was tested for its efficiency against oxidative stress in model experiments with ICR nonpregnant mice exposed either to cyclophosphamide (80 mg/kg) or whole body 60Co (6.5 Gy) irradiation. In a teratological experiment, pregnant mice were exposed to cyclophosphamide (10 mg/kg) from day 11 to 17 of gestation. Toxicity was measured by determining the lysosomal enzymes acid phosphatase and N-acetyl-beta-D-glucosaminidase. Cyclophosphamide and irradiation caused a significant increase in acid phosphatase and N-acetyl-beta-D-glucosaminidase activity in the spleen of nonpregnant mice. In the liver, lysosomal enzyme activities were unchanged and no changes in protein levels were recorded. In pregnant mice, acid phosphatase and N-acetyl-beta-D-glucosaminidase activities were increased in the spleen. An increase in foetal acid phosphatase liver activity was found. Pretreatment with stobadine prior to cyclophosphamide and irradiation significantly diminished the biochemical changes in both nonpregnant and pregnant mice. We conclude that stobadine is able to protect mice against cyclophosphamide- or irradiation-induced oxidative stress.
Navarová
J
J
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava. exfajana@savba.sk
Ujházy
E
E
Dubovický
M
M
D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002243:Carbolines / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005260:Female; D005720:Gamma Rays; D008099:Liver / Q000196:embryology / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D011247:Pregnancy; D013154:Spleen / Q000201:enzymology
D000975:Antioxidants; D002243:Carbolines; D003520:Cyclophosphamide; D000135:Acid Phosphatase; D000118:Acetylglucosaminidase; C100106:dicarbine