In vivo-induced genes in Pseudomonas aeruginosa.
In vivo expression technology was used for testing Pseudomonas aeruginosa in the rat lung model of chronic infection and in a mouse model of systemic infection. Three of the eight ivi proteins found showed sequence identity to known virulence factors involved in iron acquisition via an open reading frame (called pvdI) implicated in pyoverdine biosynthesis, membrane biogenesis (FtsY), and adhesion (Hag2).
Handfield
M
M
Microbiologie Moléculaire et Génie des Protéines, Pavillon Charles-Eugène Marchand, Faculté de Médecine, Université Laval, Sainte-Foy, Québec, Canada G1K 7P4.
Lehoux
D E
DE
Sanschagrin
F
F
Mahan
M J
MJ
Woods
D E
DE
Levesque
R C
RC
D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D015169:Colony Count, Microbial; D015723:Gene Library; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009842:Oligopeptides; D016366:Open Reading Frames; D010860:Pigments, Biological / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D011552:Pseudomonas Infections / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014774:Virulence
D001426:Bacterial Proteins; C087406:FtsY protein, Bacteria; D009842:Oligopeptides; D010860:Pigments, Biological; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; C042453:pyoverdin