A novel method for GHB detection in urine and its application in drug-facilitated sexual assaults.
A confirmation procedure for the identification and quantification of gamma-hydroxybutyric acid (GHB) in urine is presented. This method is unique in that it does not involve the conversion of GHB to the gamma-butyrolactone (GBL). The urine samples were extracted using ethyl acetate, evaporated and derivatized with N, O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS), and analyzed by gas chromatography-mass spectrometry. Quantification was performed using selective ion monitoring (SIM), using GHB-d(6) as the internal standard. This method is simple and provides excellent linearity and sensitivity for GHB in urine.
Elian
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Commonwealth of Massachusetts, State Police Crime Laboratory, Forensic Toxicology Unit, 59 Horsepond Road, Sudbury, MA 01776, USA. albert/elian@pol.state.ma.us
D000085:Acetates; D003890:Desiccation; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006885:Hydroxybutyrates / Q000009:adverse effects / Q000652:urine*; D013287:Illicit Drugs / Q000009:adverse effects / Q000652:urine*; D007202:Indicators and Reagents; D017736:Ion-Selective Electrodes; D011902:Rape; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection; D014297:Trimethylsilyl Compounds
D000085:Acetates; D006885:Hydroxybutyrates; D013287:Illicit Drugs; D007202:Indicators and Reagents; D014297:Trimethylsilyl Compounds; C111420:4-hydroxybutyric acid; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; C039293:trimethylchlorosilane; C007650:ethyl acetate