title: Synthesis and antitumor activity of duocarmycin derivatives: modification at C-8 position of A-ring pyrrole compounds bearing the simplified DNA-binding groups.
abstract: A series of the 8-O-substituted A-ring pyrrole derivatives of duocarmycin bearing the simplified DNA-binding moieties such as cinnamoyl or heteroarylacryloyl groups were synthesized, and evaluated for in vitro anticellular activity against HeLa S3 cells and in vivo antitumor activity against murine sarcoma 180 in mice. In addition, the stability of the 8-O-substituted analogues in aqueous solution and the conversion to their active form (cyclopropane compound) from the 8-O-substituted analogues in mice or human serum were examined. The 8-O-substituted A-ring pyrrole derivatives bearing the simplified DNA-binding moieties showed remarkably potent in vivo antitumor activity and low peripheral blood toxicity compared with the 8-O-substituted A-ring pyrrole derivatives having the trimethoxyindole skeleton in segment-B (Seg-B), which were equal to 8-O-[(N-methylpiperazinyl)carbonyl] derivatives of 4'-methoxycinnamates and 4'-methoxy-beta-heteroarylacrylates. Moreover, among 8-O-substituted analogues, several compounds can be chemically or enzymatically converted to their active form in human serum. This result indicated that new 8-O-substituted derivatives were different prodrugs from KW-2189 and 8-O-substituted analogues being the same type of prodrug as KW-2189.
authors.affiliation: Pharmaceutical Research Institute, Kyowa Hakko Kogyo Company,. Ltd., Sunto, Shizuoka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Amishiro
authors.forename: S
authors.initials: S
authors.lastname: Nagamura
authors.forename: C
authors.initials: C
authors.lastname: Murakata
authors.forename: A
authors.initials: A
authors.lastname: Okamoto
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Asada
authors.forename: K
authors.initials: K
authors.lastname: Gomi
authors.forename: T
authors.initials: T
authors.lastname: Tamaoki
authors.forename: M
authors.initials: M
authors.lastname: Okabe
authors.forename: N
authors.initials: N
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Saito
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001772:Blood Cell Count / Q000187:drug effects; D004247:DNA / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D011758:Pyrroles / Q000737:chemistry*; D011760:Pyrrolidinones / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014407:Tumor Cells, Cultured
chemical_list: D000903:Antibiotics, Antineoplastic; D011758:Pyrroles; D011760:Pyrrolidinones; D004247:DNA