title: Sciatic nerve of normal and T200x5R Swiss white mice fails to support multiplication of intraneurally injected M. leprae.
abstract: In a preliminary study we have shown that freshly harvested Mycobacterium leprae, when injected into the sciatic nerve in normal and immunosuppressed (TR) mice, induce massive but localized epithelioid and macrophage granuloma, respectively, in 3-4 weeks. In order to determine the fate of M. leprae injected intraneurally into normal and TR mice, in the present study we measured sequentially the viability, fold increase and clearance, if any, using semi-quantitative methods. The average M. leprae yield per nerve assessed at regular intervals, beginning at 24 hr and including 72 hr, 1 week, 2, 3, 4, 12, 24 and 48 weeks, showed neither a significant increase nor a decrease in either the normal or the TR mice. The viability of M. leprae, assessed using the standard mouse foot pad method, showed a significant decrease as compared to baseline growth effective at 24 hr and remained static until approximately 4 weeks. A further decline and total loss of viability was noted by 12 months. The results show that injection of M. leprae via the intraneural route in both normal and TR mice failed to sustain the viability and failed to support the multiplication of the organisms.
authors.affiliation: Foundation for Medical Research, Thadani Marg, Worli, Mumbai, India. frchbom@bom2.vsnl.net.in
authors.forename: V P
authors.initials: VP
authors.lastname: Shetty
authors.forename: P S
authors.initials: PS
authors.lastname: Matharu
authors.forename: N H
authors.initials: NH
authors.lastname: Antia
mesh_terms: D000818:Animals; D005260:Female; D007165:Immunosuppression Therapy; D015441:Leprosy, Tuberculoid / Q000382:microbiology* / Q000473:pathology; D008297:Male; D051379:Mice; D009166:Mycobacterium leprae / Q000254:growth & development*; D012584:Sciatic Nerve / Q000382:microbiology* / Q000473:pathology; D013934:Thymectomy; D014916:Whole-Body Irradiation