In vitro fermentation of swine ileal digesta containing oat bran dietary fiber by rat cecal inocula adapted to the test fiber increases propionate production but fermentation of wheat bran ileal digesta does not produce more butyrate.
This experiment evaluated three hypotheses: i) production of propionate is increased during fermentation of substrate containing oat bran (OB)(6); ii) production of butyrate is increased during fermentation of substrate containing wheat bran (WB) and iii) results of in vitro fermentations using physiological substrates and inocula agree with in vivo data. Ileal digesta collected from swine fed OB and WB were the substrates. Digesta was fermented for 0-96 h in an anaerobic in vitro system using inocula prepared from ceca of rats fed the same fiber sources. Carbohydrate and short-chain fatty acid (SCFA) contents in the fermentations were measured by gas chromatography. Fermentation of WB digesta did not produce more n-butyrate (P > 0.05) and was significantly slower (P < 0.05) than fermentation of OB digesta. OB digesta fermentation produced a significantly greater (P < 0.05) molar proportion of SCFA as propionate. Bacterial mass increased more and was maintained longer during fermentation of OB digesta than the WB digesta. Our results indicate that dilution of undigested WB fiber and not n-butyrate production is one mechanism by which WB may protect colonic mucosa; propionate production is increased during fermentation of beta-glucan in OB; and an in vitro system using physiological sources of inoculum and substrate containing WB and OB yields results that agree with in vivo findings in humans and rats.
Monsma
D J
DJ
Department of Nutritional Sciences. Research Animal Resources Center, the Graduate School, University of Wisconsin, Madison, 53706, USA.
Thorsen
P T
PT
Vollendorf
N W
NW
Crenshaw
T D
TD
Marlett
J A
JA
D000704:Analysis of Variance; D000818:Animals; D018554:Avena; D002087:Butyrates / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D004043:Dietary Fiber / Q000378:metabolism*; D004063:Digestion; D005232:Fatty Acids, Volatile / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D007082:Ileum / Q000378:metabolism*; D008297:Male; D011422:Propionates / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D014908:Triticum
D002087:Butyrates; D002241:Carbohydrates; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D011422:Propionates