title: A rapid and reliable solid-phase extraction--LC/MS/MS assay for the determination of two novel human leukocyte elastase inhibitors, SYN-1390 and SYN-1396, in rat plasma.
abstract: A rapid and rugged solid-phase extraction-liquid chromatographic-electrospray tandem mass spectrometric method has been developed to quantitate two novel human leukocyte elastase inhibitors, SYN-1390 and SYN-1396, in rat plasma. A reversed-phase column and an isocratic mobile phase consisting of acetonitrile-water-formic acid (70:30:0.2, v/v/v) were used. The mass spectrometer was operated in the multiple reaction monitoring mode. For both analytes, standard curves were linear over a working range of 0.1-20 microg ml(-1) (r> or =0.995) and the limit of quantitation was 0.1 microg ml(-1) with a 150 microl plasma volume. This assay proved to be useful for the determination of SYN-1390 and SYN-1396 in plasma samples from pharmacokinetic study.
authors.affiliation: Department of Biopharmaceutics and Pharmacokinetics, SynPhar Laboratories, Inc., Edmonton, Alberta, Canada.
authors.forename: J K
authors.initials: JK
authors.lastname: Khan
authors.forename: H Z
authors.initials: HZ
authors.lastname: Bu
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Samarendra
authors.forename: N
authors.initials: N
authors.lastname: Maiti
authors.forename: R G
authors.initials: RG
authors.lastname: Micetich
mesh_terms: D000818:Animals; D002511:Cephalosporins / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D015843:Serpins / Q000097:blood* / Q000737:chemistry
chemical_list: D002511:Cephalosporins; C407749:SYN 1390; C407748:SYN 1396; D015843:Serpins