Intraventricular infusion of apolipoprotein E ameliorates acute neuronal damage after global cerebral ischemia in mice.
The ability of intraventricular infusion of apolipoprotein E (apoE) to reduce neuronal damage after global cerebral ischemia was investigated in apoE-deficient and wild-type mice. ApoE (5 microg/mL lipid-conjugated derived from human plasma; 1 microL/h, continuous infusion) significantly reduced neuronal damage in the caudate nucleus and CA2 pyramidal cell layer by approximately 50% in apoE-deficient mice after global ischemia compared to vehicle infusion. In wild-type mice infused with apoE, there was a trend for ischemic neuronal damage to be reduced. ApoE-infused mice had a marked reduction in 4-hydroxynonenal immunoreactivity, as a marker of lipid peroxidation. The results show that the presence of apoE at or after the time of injury can be neuroprotective, possibly via an anti-oxidant mechanism.
Horsburgh
K
K
University Department of Neuropathology, Institute of Neurological Sciences, South Glasgow University Hospitals NHS Trust, University of Glasgow, UK.
McCulloch
J
J
Nilsen
M
M
McCracken
E
E
Large
C
C
Roses
A D
AD
Nicoll
J A
JA
D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001057:Apolipoproteins E / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000378:metabolism / Q000473:pathology*; D002421:Caudate Nucleus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D006801:Humans; D007276:Injections, Intraventricular; D008054:Lipid Peroxides / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D009474:Neurons / Q000187:drug effects* / Q000473:pathology*; D017966:Pyramidal Cells / Q000187:drug effects / Q000473:pathology
D000447:Aldehydes; D001057:Apolipoproteins E; D008054:Lipid Peroxides; C027576:4-hydroxy-2-nonenal