Separation of nontarget compounds by DNA aptamers.
The ability of DNA aptamers to separate nontarget compounds is demonstrated. Two G-quarter forming aptamers, a 15-mer and a 20-mer, were covalently linked to fused silica capillary columns to serve as stationary-phase reagents in capillary electrochromatography. Separations of binary mixtures of amino acids (D-trp and D-tyr), enantiomers (D-trp and L-trp), and polycyclic aromatic hydrocarbons were achieved. Aptamers offer several attractive features for stationary-phase reagents, including ease of synthesis and of attachment to surfaces and modification of their binding properties through minor changes in sequence.
Kotia
R B
RB
Department of Chemistry, P. M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708-0346, USA.
Li
L
L
McGown
L B
LB
D004247:DNA / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification*; D013237:Stereoisomerism
D009841:Oligonucleotides; D011084:Polycyclic Aromatic Hydrocarbons; D004247:DNA