A forced-flow membrane reactor for transfructosylation using ceramic membrane.
A forced-flow membrane reactor system for transfructosylation was investigated using several ceramic membranes having different pore sizes. beta-Fructofuranosidase from Aspergillus niger ATCC 20611 was immobilized chemically to the inner surface of a ceramic membrane activated by a silane-coupling reagent. Sucrose solution was forced through the ceramic membrane by crossflow filtration while transfructosylation took place. The saccharide composition of the product, which was a mixture of fructooligosaccharides (FOS), was a function of the permeate flux, which was easily controlled by pressure. Using 0.2 micrometer pore size of symmetric ceramic membrane, the volumetric productivity obtained was 3.87 kg m(-3) s(-1), which was 560 times higher than that in a reported batch system, with a short residence time of 11 s. The half-life of the immobilized enzyme in the membrane was estimated to be 35 days by a long-term operation.
Nishizawa
K
K
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, 2-1-2, Kannondai, Tsukuba-shi, Ibaraki, 305-8642, Japan.
Nakajima
M
M
Nabetani
H
H
D001234:Aspergillus niger / Q000201:enzymology*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002516:Ceramics; D004800:Enzymes, Immobilized; D005632:Fructose / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005632:Fructose