Fluorimetric determination of prenalterol hydrochloride in pharmaceuticals and biological fluids based on its oxidation reaction by hexacyanoferrate(III).
A rapid and sensitive fluorimetric method for the determination of prenalterol hydrochloride is presented, based on the oxidation of the analyte with potassium hexacyanoferrate(III) in a slightly alkaline medium (pH 9.23). The different experimental parameters were carefully studied and incorporated into the procedure. The oxidation product exhibits a blue fluorescence with its emission maximum at 427 nm, and excitation maximum at 314 nm. Fluorescence intensity is a linear function of prenalterol hydrochloride concentration over the range of 0.2-3.6 microg/ml(-1) in the solution finally measured. The method was successfully applied to the determination of prenalterol hydrochloride in pharmaceutical formulations and biological fluids. A proposal for the reaction pathway is suggested.
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Department of Chemistry, College of Science, Women Student-Medical Studies and Sciences Sections, King Saud University, Riyadh, Saudi Arabia.
D001826:Body Fluids / Q000737:chemistry; D002138:Calibration; D005295:Ferrocyanides; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D011294:Prenalterol / Q000032:analysis* / Q000097:blood / Q000652:urine; D013566:Sympathomimetics / Q000032:analysis / Q000097:blood / Q000652:urine
D005295:Ferrocyanides; D013566:Sympathomimetics; C116383:ferrocyn; D011294:Prenalterol