Epithelial cell kinetics in the inflammatory process of chicken trachea infected with infectious bronchitis virus.
All stages of degeneration and regeneration in chicken tracheal epithelium were studied morphologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 1 to 7 days post-inoculation (d.p.i.) with a peak on 3 d.p.i. At 1 d.p.i., almost all epithelial cells were involved in the degeneration. At this time, labelling index of bromodeoxyuridine (BrdU) in the basal cells showed significantly high value compared with control. At 2 and 3 d.p.i., a great number of basal cells were recognized, but the BrdU labelling index tended to decrease. At 4 and 5 d.p.i., the BrdU labelling index of basal cells significantly decreased than 1 d.p.i., and a few number of regenerated immature ciliated epithelia appeared. At 6 to 11 d.p.i., the ciliated columnar epithelia increased rapidly in number, and returned to the normal appearance except for non-ciliated patch by 13 d.p.i. These results suggested that the tracheal epithelial cells infected with IBV degenerated within 24 hours and proliferating activity of basal cells functioned immediately, and 3 to 4 days later, these basal cells were differentiated to the ciliated epithelia.
Kotani
T
T
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Shiraishi
Y
Y
Tsukamoto
Y
Y
Kuwamura
M
M
Yamate
J
J
Sakuma
S
S
Gohda
M
M
D000818:Animals; D002645:Chickens; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary*; D004847:Epithelial Cells / Q000473:pathology; D001351:Infectious bronchitis virus; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D009092:Mucous Membrane / Q000473:pathology; D011201:Poultry Diseases / Q000473:pathology* / Q000821:virology; D014132:Trachea / Q000473:pathology*; D014136:Tracheitis / Q000473:pathology / Q000662:veterinary* / Q000821:virology