Effect of solvents on the fumonisins analysis by high-performance liquid chromatography with AccQ.Fluor as the derivatizing reagent.
The effect of several solvent systems on the chromatographic response of fumonisin B1 and B2 derived with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AccQ.Fluor) is described. Naturally contaminated corn samples were extracted and purified by a standard method. Then, samples were dissolved in different solvents, derived with AccQ.Fluor reagent and analysed using HPLC. Results were solvent dependent, methanol being the best one among all assayed solvents for both fumonisins studied and acetonitrile the poorest. o-Phthaldialdehyde (OPA) reagent was used as a reference method.
Velázquez
C
C
Phytochemistry Department, Facultad de Ciencias Químicas, Universidad Nacional de Asunción, Paraguay.
Llovera
M
M
Plana
J
J
Canela
R
R
D000634:Aminoquinolines / Q000737:chemistry*; D002219:Carbamates / Q000737:chemistry*; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037341:Fumonisins; D007202:Indicators and Reagents / Q000737:chemistry*; D012015:Reference Standards; D012997:Solvents; D013050:Spectrometry, Fluorescence
C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000634:Aminoquinolines; D002219:Carbamates; D002264:Carboxylic Acids; D037341:Fumonisins; D007202:Indicators and Reagents; D012997:Solvents; C056934:fumonisin B2; C056933:fumonisin B1