title: Scanning electron microscopic observations of human dentine after mechanical caries excavation.
abstract: OBJECTIVE
  The structural integrity and surface characteristics of dentine remaining after caries excavation may be relevant to the subsequent bonding of adhesive restorative materials to the prepared cavity. This in vitro investigation aimed to analyse the different surface characteristics of the dentine cavity floor created after preparation using five different mechanical and chemo-mechanical methods of excavation: hand excavation, slow-speed bur, sono-abrasion, air-abrasion and Carisolv gel.
  
  
  METHODS
  Ten cavities were prepared using each excavation method in extracted teeth with occlusal carious lesions. Epoxy resin replicas of the 50 cavities were manufactured from silicone impressions and then analysed using secondary electron scanning electron microscopy (SEM) to ascertain the surface characteristics of the dentine at the cavity floor.
  
  
  CONCLUSIONS
  Results from the 50 cavities examined suggested that each alternative excavation technique produced a different and characteristic dentine surface. Carisolv gel was the only method examined that consistently removed the smear layer during excavation to leave exposed dentine tubules at the end of cavity preparation.
authors.affiliation: Division of Conservative Dentistry, Guy's, King's and St. Thomas' Dental Institute, Guy's Dental Hospital, London, UK. avijit.banerjee@kcl.ac.uk
authors.forename: A
authors.initials: A
authors.lastname: Banerjee
authors.forename: E A
authors.initials: EA
authors.lastname: Kidd
authors.forename: T F
authors.initials: TF
authors.lastname: Watson
mesh_terms: D020906:Air Abrasion, Dental / Q000295:instrumentation / Q000379:methods; D003731:Dental Caries / Q000473:pathology / Q000628:therapy*; D003737:Dental Cavity Preparation / Q000295:instrumentation / Q000379:methods*; D003804:Dentin / Q000648:ultrastructure*; D005069:Evaluation Studies as Topic; D005782:Gels; D018698:Glutamic Acid / Q000627:therapeutic use; D006801:Humans; D066298:In Vitro Techniques; D007930:Leucine / Q000627:therapeutic use; D008239:Lysine / Q000627:therapeutic use; D008334:Mandible; D008855:Microscopy, Electron, Scanning; D008963:Molar; D013010:Sonication / Q000295:instrumentation; D013499:Surface Properties
chemical_list: C119934:Carisolv; D005782:Gels; D018698:Glutamic Acid; D007930:Leucine; D008239:Lysine