title: Analysis of the fine specificity of Tn-binding proteins using synthetic glycopeptide epitopes and a biosensor based on surface plasmon resonance spectroscopy.
abstract: Using synthetic Tn (GalNAc-O-Ser/Thr) glycopeptide models and a biosensor based on surface plasmon resonance spectroscopy we have determined that isolectin B4 from Vicia villosa (VVLB4) binds to one Tn determinant whereas the anti-Tn monoclonal antibodies 83D4 and MLS128 require at least two Tn residues for recognition. When an unglycosylated amino acid is introduced between the Tn residues, both antibodies do not bind. MLS128 affinity was higher on a glycopeptide with three consecutive Tn residues. These results indicate that Tn residues organized in clusters are essential for the binding of these antibodies and indicate a different Tn recognition pattern for VVLB4.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Av. Gral. Flores 2125, CP 11800, Montevideo, Uruguay. eosinaga@lobbm.fmed.edu.uy
authors.forename: E
authors.initials: E
authors.lastname: Osinaga
authors.forename: S
authors.initials: S
authors.lastname: Bay
authors.forename: D
authors.initials: D
authors.lastname: Tello
authors.forename: A
authors.initials: A
authors.lastname: Babino
authors.forename: O
authors.initials: O
authors.lastname: Pritsch
authors.forename: K
authors.initials: K
authors.lastname: Assemat
authors.forename: D
authors.initials: D
authors.lastname: Cantacuzene
authors.forename: H
authors.initials: H
authors.lastname: Nakada
authors.forename: P
authors.initials: P
authors.lastname: Alzari
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology; D015374:Biosensing Techniques; D000939:Epitopes / Q000737:chemistry; D006020:Glycopeptides / Q000138:chemical synthesis / Q000276:immunology; D006031:Glycosylation; D007700:Kinetics; D037102:Lectins / Q000276:immunology; D051379:Mice; D037121:Plant Lectins; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D000939:Epitopes; D006020:Glycopeptides; D037102:Lectins; D037121:Plant Lectins; C040286:Tn antigen; C042242:Vicia lectins