title: Protein extraction from Atriplex lampa leaves: potential use as forage for animals used for human diets.
abstract: The purpose of this study was to evaluate the effect of pH on the extraction of protein nitrogen from Atriplex lampa leaves (Moquin) Dietrich. The chemical characterization of the dry matter indicated the following (g/100 g): protein, 26.93; ash, 21.80; ether extract, 4.65; dry matter, 37.30; sodium, 6.05; and calcium, 0.41. Non-critical values were obtained for saponins and nitrates. The high concentration of oxalic acid (8.52 g/100 g), together with elevated salt content account for the low palatability of the studied species. In order to determine the parameters needed to improve the extraction in protein nitrogen from leaves, fresh material was macerated with 2% sodium metasulfite, followed by pulping with a hand-driven grinder. Extractions were performed at different pH values (2-12) adjusting the value with 5N HCL or NaOH, with agitation followed by centrifugation and pressing. Supernatants were collected and kept. The last extraction was performed with Tween 20 in order to obtain maximum nitrogen recovery from the residue cake. Highest extraction (41.23%) was obtained at pH 10 with a 1:5 ratio (leaf: deionized water, w/v). It is proposed that this regional natural resource may be used to elaborate a protein concentrate, which can be made more palatable by decreasing potassium and sodium salt content with the use of membrane technology.
authors.affiliation: Departamento de Farmacia, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Argentina.
authors.forename: S S
authors.initials: SS
authors.lastname: Fernández
authors.forename: A P
authors.initials: AP
authors.lastname: Padilla
authors.forename: S
authors.initials: S
authors.lastname: Mucciarelli
mesh_terms: D000821:Animal Feed; D000818:Animals; D004044:Dietary Proteins / Q000302:isolation & purification*; D005502:Food; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification*
chemical_list: D004044:Dietary Proteins; D010940:Plant Proteins