title: cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stability of the expressed enzyme, and polymorphism among strains.
abstract: Pig testicular lactate dehydrogenase-C (LDHC) cDNA was cloned and sequenced. The deduced sequence of 332 amino acids from pig LDHC shows 73% and 67% identity with that of pig LDHA (muscle) and LDHB (heart) respectively, whereas pig LDHA and LDHB isozymes shows 74% sequence identity. Pig and mouse LDHC cDNAs were subcloned into bacterial expression vector, and the expressed pig LDHC isozyme was shown to be as thermally stable as mouse LDHC isozyme. Pig genomic DNAs from Chinese Meishan, English Yorkshire, Danish Landrace and American Duroc were shown to exhibit polymorphic sites for restriction enzymes EcoRI, BamHI and PstI.
authors.affiliation: Institute of Biomedical Sciences, National Sun Yat-Sen University, Taiwan, ROC.
authors.forename: H W
authors.initials: HW
authors.lastname: Huang
authors.forename: T Z
authors.initials: TZ
authors.lastname: Liu
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.forename: C F
authors.initials: CF
authors.lastname: Tu
authors.forename: W C
authors.initials: WC
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Shimogiri
authors.forename: H
authors.initials: H
authors.lastname: Mannen
authors.forename: S S
authors.initials: SS
authors.lastname: Li
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004795:Enzyme Stability; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D006358:Hot Temperature; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013552:Swine; D013696:Temperature; D013737:Testis / Q000201:enzymology*
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D004247:DNA; D007770:L-Lactate Dehydrogenase