Comparison of polymorphonuclear cells from healthy donors and differentiated HL-60 cells as phagocytes in an opsonophagocytic assay using antigen-coated fluorescent beads.
Polymorphonuclear cells (PMNs) from healthy donors and differentiated HL-60 cells were compared in an opsonophagocytic assay using fluorescent latex beads coated with Streptococcus pneumoniae polysaccharide conjugates. Serum-specific phagocytosis was efficiently mediated by both sources of cells, as measured by flow cytometry, but the mean number of beads ingested per cell was three- to fivefold higher when PMNs were used than when HL-60 cells were used. Nevertheless, differentiated HL-60 cells could be a convenient and standardized source of cells to evaluate the functionality of specific antibodies to vaccine candidates as a coating on fluorescent beads.
Guy
B
B
Research Department, Aventis Pasteur, Campus Merieux, 69280 Marcy L'Etoile, France. Bruno.Guy@aventis.com
Testart
C
C
Gimenez
S
S
Sanchez
V
V
Lheritier
P
P
Rossin
D
D
Mignon
M
M
Danve
B
B
Trannoy
E
E
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D016667:Bacterial Capsules / Q000276:immunology; D001782:Blood Donors; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006304:Health Status; D006801:Humans; D008863:Microspheres; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins; D010586:Phagocytes; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011817:Rabbits; D013296:Streptococcus pneumoniae / Q000276:immunology
D000942:Antigens, Bacterial; D005456:Fluorescent Dyes; D009895:Opsonin Proteins; D011135:Polysaccharides, Bacterial; C056696:pneumococcal polysaccharide, type 14; C053136:pneumonococcal polysaccharide, type 4; D016650:Fluorescein-5-isothiocyanate