Functional and pharmacological properties of GABArho1delta51 receptors.
Gamma-aminobutyrate is the main inhibitory neurotransmitter in the vertebrate brain, and the gamma-aminobutyric acid (GABA) receptor subunit GABArho1delta51 is an alternatively spliced form of the GABArho1 receptor that was recently isolated from human retina cDNA libraries. The rho1delta51 receptor subunit lacks 17 amino acids in the extracellular N-terminal domain and, when expressed in Xenopus oocytes, forms functional homomeric GABA receptors. Unexpectedly, even after a such a big deletion, the fundamental properties of the deleted variant receptors are very similar to those of the complete GABArho1 receptors. For example, both types of receptors are bicuculline resistant, desensitize very little, and are negatively modulated by Zn2+ and positively modulated by La3+. In spite of such similarities, the GABArho1delta51 receptors are more sensitive to GABA, to the specific GABA(C) antagonist (1,2,5,6-tetrahydropyridine-4-yl)methylphosphinic acid and to Zn2+, than the complete GABArho1 receptors. The GABArho1delta51 receptors extend the variety of inhibitory receptors in the retina. Their functional significance still remains to be determined.
Demuro
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Department of Neurobiology and Behavior, University of California, Irvine 92697, USA.
Martínez-Torres
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Miledi
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D000409:Alanine / Q000494:pharmacology; D000818:Animals; D005998:Glycine / Q000494:pharmacology; D007700:Kinetics; D007811:Lanthanum / Q000494:pharmacology; D018079:Receptors, GABA / Q000187:drug effects* / Q000378:metabolism; D014982:Xenopus laevis; D015032:Zinc / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000494:pharmacology*
D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid; D007811:Lanthanum; D015032:Zinc; D000409:Alanine; D005998:Glycine