title: Effect of glutathione-modulating compounds on platinum compounds-induced cytotoxicity in human glioma cell lines.
abstract: The relation between the effect of glutathione(GSH)-modulating compounds and platinum compounds (Cisplatin, Nedaplatin)-induced cytotoxicity was investigated. Pretreatment of human glioblastoma (T98G, U87MG) and glioma (KG1C) cell lines with L-buthionine-[S,R]-sulfoximine, which decrease the intracellular GSH concentration, remarkably increased their sensitivity against platinum compounds, whereas pretreatment with N-acetyl-L-cysteine, which increase the intracellular GSH concentration, only marginally protected the cells from the cytotoxic effect of platinum compounds. The results suggest that platinum compounds-induced cytotoxicity can be modified by GSH-modulating compounds in glioblastoma and glioma cell lines.
authors.affiliation: Department of Neurosurgery, Tokyo Metropolitan Ebara Hospital, Japan. masaiida-e@ebara-hp.ota.tokyo.jp
authors.forename: M
authors.initials: M
authors.lastname: Iida
authors.forename: H
authors.initials: H
authors.lastname: Doi
authors.forename: S
authors.initials: S
authors.lastname: Asamoto
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Sakagami
authors.forename: N
authors.initials: N
authors.lastname: Kuribayashi
authors.forename: M
authors.initials: M
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Okamura
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D001932:Brain Neoplasms / Q000473:pathology*; D019328:Buthionine Sulfoximine / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D005910:Glioma / Q000473:pathology*; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D019328:Buthionine Sulfoximine; C053989:nedaplatin; D005978:Glutathione; D002945:Cisplatin; D000111:Acetylcysteine