Isotope-labeled immunoassays without radiation waste.
The practice of immunoassay has experienced a widespread transition from radioisotopic labeling to nonisotopic labeling over the last two decades. Radioisotope labels have drawbacks that hamper their applications: (i) perceived radiation hazards of reagents, (ii) regulatory requirements and disposal problems of working with radioactive materials, and (iii) short shelf-life of the labeled reagents. The advantage of isotopic labeling is the incorporation into analytes without altering structure or reactivity, as is often the case with ELISA or fluorescent detection systems. We developed a format for isotope label immunoassay with the long-life isotope (14)C as the label and accelerator mass spectrometer (AMS) as the detection system. AMS quantifies attomole levels of several isotopes, including (14)C. With this exquisite sensitivity, the sensitivity of an immunoassay is limited by the K(d) of the antibody and not the detection system. The detection limit of the assays for atrazine and 2,3,7,8-tetrachlorodibenzo-p-dioxin was 2.0 x 10(-10) M and 2.0 x 10(-11) M, respectively, approximately an order of magnitude below the standard enzyme immunoassay. Notably, <1 dpm (0.45 pCi) of (14)C-labeled compound was used in each assay, which is well below the limit of disposal (50 nCi per g) as nonradioactive waste. Thus, endogenous reporter ligands quantified by AMS provide the advantages of an RIA without the associated problems of radioactive waste.
Shan
G
G
Department of Entomology, University of California, Davis, CA 95616, USA.
Huang
W
W
Gee
S J
SJ
Buchholz
B A
BA
Vogel
J S
JS
Hammock
B D
BD
D000818:Animals; D000915:Antibody Affinity; D001280:Atrazine / Q000032:analysis; D002250:Carbon Radioisotopes; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D005240:Feasibility Studies; D006241:Haptens / Q000378:metabolism; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis; D011850:Radioactive Waste / Q000517:prevention & control*
D002250:Carbon Radioisotopes; D006241:Haptens; D007074:Immunoglobulin G; D000072317:Polychlorinated Dibenzodioxins; D011850:Radioactive Waste; D001280:Atrazine