Human megakaryocyte polyploidization is associated with a decrease in GPIIIA expression.
Megakaryocytes are platelet forming cells and are characterized by polyploidization, a phenomenon by which nuclear division occurs without corresponding cytoplasmic separation. Among the markers allowing to identify megakaryocytes, glycoprotein (GP) IIIa with GPIb and GPIIb are the most important. Using GPIIIa as a marker to recognize megakaryocytes in the bone marrow, we have estimated GPIIIa expression by flow cytometry in megakaryocyte populations from normal individuals and from patients with chronic myelogenous leukemia, immune thrombocytopenic purpura or polycythemia vera. We showed that the expression of GPIIIa is decreasing during megakaryocyte polyploidization in normal and pathological situations.
Baatout
S
S
Laboratory of Experimental Hematology and Oncology, Catholic University of Louvain, Brussels, Belgium.
Chatelain
B
B
Staquet
P
P
Symann
M
M
Chatelain
C
C
D015703:Antigens, CD / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D039661:Integrin beta3; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology; D008533:Megakaryocytes / Q000378:metabolism* / Q000473:pathology; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011087:Polycythemia Vera / Q000378:metabolism / Q000473:pathology; D011123:Polyploidy; D011696:Purpura, Thrombocytopenic / Q000378:metabolism / Q000473:pathology
D015703:Antigens, CD; D039661:Integrin beta3; D010980:Platelet Membrane Glycoproteins