Dietary docosahexaenoic acid enhances ferric nitrilotriacetate-induced oxidative damage in mice but not when additional alpha-tocopherol is supplemented.
Weaning mice were fed a diet supplemented with beef tallow (BT) or BT plus docosahexaenoic acid (DHA) containing 100 mg alpha-tocopherol/kg (alpha-Toc100) or 500 mg alpha-tocopherol/kg (alpha-Toc500) for 4 wk to modify membrane fatty acid unsaturation, and then were administered ferric nitrilotriacetate (Fe-NTA). The mortality caused by Fe-NTA was higher in the group fed the DHA (alpha-Toc100) diet than in the BT diet groups but the DHA (alpha-Toc500) diet suppressed this increase. Serum and kidney alpha-tocopherol contents were slightly influenced by the dietary fatty acids but not significantly. These results indicate that the increased unsaturation of tissue lipids enhances oxidative damage induced by Fe-NTA in mice fed DHA (alpha-Toc100) but not when additional alpha-tocopherol is supplemented. The apparent discrepancy between the observed enhancement by dietary DHA of oxidative damage and the beneficial effects of dietary DHA on the so-called free radical diseases is discussed in terms of strong bolus oxidative stress and moderate chronic oxidative stress.
Yasuda
S
S
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Watanabe
S
S
Kobayashi
T
T
Hata
N
N
Misawa
Y
Y
Utsumi
H
H
Okuyama
H
H
D000818:Animals; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage* / Q000633:toxicity*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000633:toxicity*; D004347:Drug Interactions; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D009571:Nitrilotriacetic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005290:Ferric Compounds; D010743:Phospholipids; D014810:Vitamin E; D004281:Docosahexaenoic Acids; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate