title: VPREB3: cDNA characterization and expression in human and chromosome mapping in human and mouse.
abstract: The pre-B cell receptor (pre-BCR) regulates pre-B cell expansion and allelic exclusion at the immunoglobulin (Ig) heavy chain locus and mediates the selection of Ig heavy chain variable gene segments. During the early phase of pre-BCR assembly in the mouse, the membrane Ig mu heavy chain transiently associates with the VPREB3 protein in the endoplasmic reticulum. Here, we present the human VPREB3 cDNA sequence and its B cell-specific expression in hematopoietic cell lines. We have localized this gene to chromosome 22q11 close to IGLL genes in human and to chromosome 10C in mouse.
authors.affiliation: Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Marseille, France. rosnet@ciml.univ-mrs.fr
authors.forename: O
authors.initials: O
authors.lastname: Rosnet
authors.forename: M G
authors.initials: MG
authors.lastname: Mattéi
authors.forename: O
authors.initials: O
authors.lastname: Delattre
authors.forename: C
authors.initials: C
authors.lastname: Schiff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D054420:Pre-B Cell Receptors; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011947:Receptors, Antigen, B-Cell
chemical_list: D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D054420:Pre-B Cell Receptors; D012333:RNA, Messenger; D011947:Receptors, Antigen, B-Cell; C515476:pre-B lymphocyte protein 3, human