Snake venom proteases affecting hemostasis and thrombosis.
The structure and function of snake venom proteases are briefly reviewed by putting the focus on their effects on hemostasis and thrombosis and comparing with their mammalian counterparts. Up to date, more than 150 different proteases have been isolated and about one third of them structurally characterized. Those proteases are classified into serine proteases and metalloproteinases. A number of the serine proteases show fibrin(ogen)olytic (thrombin-like) activities, which are not susceptible to hirudin or heparin and perhaps to most endogenous serine protease inhibitors, and form abnormal fibrin clots. Some of them have kininogenase (kallikrein-like) activity releasing hypotensive bradykinin. A few venom serine proteases specifically activate coagulation factor V, protein C, plasminogen or platelets. The venom metalloproteinases, belonging to the metzincin family, generally show fibrin(ogen)olytic and extracellular matrix-degrading (hemorrhagic) activities. A few venom metalloproteinases show a unique substrate specificity toward coagulation factor X, platelet membrane receptors or von Willebrand factor. A number of the metalloproteinases have chimeric structures composed of several domains such as proteinase, disintegrin-like, Cys-rich and lectin-like domains. The disintegrin-like domain seems to facilitate the action of those metalloproteinases by interacting with platelet receptors. A more detailed analysis of snake venom proteases should find their usefulness for the medical and pharmacological applications in the field of thrombosis and hemostasis.
Matsui
T
T
Division of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi, Japan.
Fujimura
Y
Y
Titani
K
K
D000595:Amino Acid Sequence; D000818:Animals; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D006487:Hemostasis / Q000187:drug effects; D006801:Humans; D008666:Metalloendopeptidases / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000737:chemistry / Q000378:metabolism; D012910:Snake Venoms / Q000737:chemistry / Q000378:metabolism*; D013917:Thrombin / Q000737:chemistry / Q000378:metabolism; D013927:Thrombosis / Q000209:etiology
D012910:Snake Venoms; D010450:Endopeptidases; D012697:Serine Endopeptidases; D013917:Thrombin; D008666:Metalloendopeptidases; C115407:acutolysin A; C089232:atrolysin C; C028220:Crotalus adamanteus proteinase II