Improvement of the identification of staphylococci isolated from bovine mammary infections using molecular methods.
Fifty-six Staphylococcus strains isolated from cases of bovine mammary infections were identified by using phenotypic and genotypic methods. Twenty-eight strains (50%) were identified at the species level according to their phenotypic characteristics, whereas the remaining 28 strains presented atypical or unreliable profiles. A combination of phenotypic and genotypic methods allowed the 56 strains studied to be classified. Internal transcribed spacer-polymerase chain reaction (ITS-PCR) based on the polymorphism of the 16S-23S rDNA spacer region appeared as a rapid and reliable method for the classification of bovine staphylococcal isolates at the species and subspecies levels.
Bes
M
M
Centre National de Référence des Toxémies à Staphylocoques, UPRES EA 1655, Laboratoire de Bactériologie, Faculté de Médecine R.T.H. Laennec, Lyon, France.
Guérin-Faublée
V
V
Meugnier
H
H
Etienne
J
J
Freney
J
J
D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial / Q000662:veterinary; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D005260:Female; D005838:Genotype; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic / Q000235:genetics; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal