Radioimmunoassay for a novel benzodiazepine inverse agonist, S-8510, in human plasma and urine.
A radioimmunoassay (RIA) was developed for a new benzodiazepine inverse agonist, 2-(isoxazol-3-yl)-3,6,7,9-tetrahydroimidazo [4,5-d] pyrano [4,3-b] pyridine monophosphate monohydrate (S-8510), in human plasma and urine. For competitive RIA, three amino derivatives of S-8510 were labelled by the Bolton and Hunter method and rabbit antisera were prepared using three immunogens, conjugates of three carboxyl derivatives of S-8510 with bovine serum albumin. All combinations of the labelled antigens and antisera were examined and homologous combinations were selected for the competitive RIA. One of the three homologous combinations had the best selectivity after investigations of cross-reactivity using 12 related compounds and was very sensitive for S-8510. Next, a pretreatment for biological samples was developed using mixed mode solid-phase extraction (SPE) column followed by the RIA (SPE/RIA). The assay recoveries for human plasma and urine were both excellent and the limits of quantitation were extremely low, 80 and 200 pg ml(-1), respectively. Human plasma samples and urine samples after administration of this drug were successfully measured by the SPE/RIA. No cross-reactive metabolites were detected in any fractions after RP-HPLC separation of the plasma samples. The RIA using carefully selected antiserum and labelled antigen was highly specific for unchanged S-8510. To simplify the RIA procedure, a scintillation proximity assay (SPA) using the same labelled antigen and antiserum was developed for analyzing S-8510 in human plasma and found to be very promising.
Kominami
G
G
Shionogi Research Laboratories, Toyonaka, Osaka, Japan. goro.kominami@shionogi.co.jp
Nakamura
M
M
Chomei
N
N
Takada
S
S
D000918:Antibody Specificity; D001569:Benzodiazepines / Q000032:analysis* / Q000097:blood / Q000652:urine; D058785:GABA-A Receptor Agonists; D006801:Humans; D007202:Indicators and Reagents; D018697:Nootropic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000032:analysis; D012711:Serum Albumin, Radio-Iodinated
D058785:GABA-A Receptor Agonists; D007202:Indicators and Reagents; D018697:Nootropic Agents; C105893:S 8510; D012711:Serum Albumin, Radio-Iodinated; D001569:Benzodiazepines; D012710:Serum Albumin, Bovine