title: Kinetic study of porcine kidney betaine aldehyde dehydrogenase.
abstract: Porcine kidney betaine aldehyde dehydrogenase (EC 1.2.1.8) kinetic properties were determined at low substrate concentrations. The double-reciprocal plots of initial velocity versus substrate concentration are linear and intersect at the left of the 1/v axis and showed substrate inhibition with betaine aldehyde. Studies of inhibition by NADH and dead-end analogs showed that NADH is a mixed inhibitor against NAD(+) and betaine aldehyde. AMP is competitive with respect to NAD(+) and mixed with betaine aldehyde. Choline is competitive against betaine aldehyde and uncompetitive with respect to NAD(+). The kinetic behavior is consistent with an Iso-Ordered Bi-Bi Steady-State mechanism.
authors.affiliation: Dirección de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo, Sonora, CP 83100, Mexico.
authors.forename: C G
authors.initials: CG
authors.lastname: Figueroa-Soto
authors.forename: E M
authors.initials: EM
authors.lastname: Valenzuela-Soto
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D050728:Betaine-Aldehyde Dehydrogenase; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D013379:Substrate Specificity; D013552:Swine
chemical_list: D009243:NAD; D000445:Aldehyde Oxidoreductases; D050728:Betaine-Aldehyde Dehydrogenase