Expression of cancer-testis antigens in lung cancer: definition of bromodomain testis-specific gene (BRDT) as a new CT gene, CT9.
In an effort to define new cancer-testis (CT) genes, we investigated whether BRDT, a testis-restricted member of the RING3 family of transcriptional regulators, is also expressed in cancer. Standard RT-PCR expression analysis detected BRDT transcripts in 12 of 47 cases of non-small cell lung cancer and single cases of both squamous cell carcinoma of the head and neck (1/12) and esophagus (1/12) but not in melanoma or in cancers of the colon, breast, kidney and bladder. Typing of 33 non-small cell lung cancers for coexpression of a panel of CT antigens revealed a high incidence (60%) of MAGE-3 mRNA expression, followed by MAGE-1 (36%), CT7/MAGE-C1 (30%), CT10 (30%), SSX4 (23%), BRDT (21%), NY-ESO-1 (21%) and HOM-MEL-40/SSX2 (15%). The coexpression pattern of these antigens provides a foundation for developing a polyvalent lung cancer vaccine.
Scanlan
M J
MJ
Ludwig Institute for Cancer Research, New York Branch at Memorial Sloan-Kettering Cancer Center, New York 10021, USA. scanlanm@mskcc.org
Altorki
N K
NK
Gure
A O
AO
Williamson
B
B
Jungbluth
A
A
Chen
Y T
YT
Old
L J
LJ
D000951:Antigens, Neoplasm / Q000235:genetics*; D019496:Cancer Vaccines / Q000276:immunology; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D013737:Testis / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D000092468:Bromodomain Containing Proteins
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D009687:Nuclear Proteins; D012333:RNA, Messenger; C109570:BRDT protein, human; D000092468:Bromodomain Containing Proteins