Cycloamylose (cyclodextrin) glucanotransferase degrades intact granules of potato raw starch.
Cycloamylose (cyclodextrin) glucanotransferase (EC 2.4.1.19, CGTase) originated from Bacillus macerans degraded intact granules of potato raw starch and converted them into cyclodextrins (CDs). The degradation required sufficient stirring of starch-CGTase suspension. The morphology of the degraded starch granules was unique; that is, the inner part of the granules was observed by scanning electron microscope to be more susceptible to CGTase than the outer part. Effects of pH, temperature, starch concentration, and enzyme amount on CD production were studied.
Yamamoto
K
K
National Food Research Institute, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan. kazutaka@nfri.affrc.go.jp
Zhang
Z Z
ZZ
Kobayashi
S
S
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000378:metabolism; D005524:Food Technology / Q000379:methods; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011198:Solanum tuberosum / Q000378:metabolism* / Q000648:ultrastructure; D013213:Starch / Q000378:metabolism* / Q000648:ultrastructure
D003505:Cyclodextrins; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase