title: The genetic analysis of F1 hybrid larvae between female Trichinella spiralis and male Trichinella britovi.
abstract: Hybrids between female Trichinella spiralis and male Trichinella britovi were constructed. Then, hybrid genotype was characterized by DNA markers including mitochondrial cytochrome c oxidase subunit I (CO I) gene, the gene encoding the 43-kDa excretory-secretory (ES) protein, and genomic DNA fragments specific for T. spiralis and T. britovi identified from random amplified polymorphism DNA (RAPD). PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial CO I gene revealed that all hybrids carried a T. spiralis pattern. The same analysis of the gene encoding the 43-kDa ES protein showed that each hybrid carried both T. spiralis and T. britovi gene type simultaneously. In the analysis of genomic DNA using RAPD-derived PCR primers, some hybrids carried T. spiralis and T. britovi-specific RAPD markers, while others carried the RAPD marker of T. spiralis only.
authors.affiliation: Department of Parasitology, Gifu University School of Medicine, Tsukasa 40, Gifu 500-8705, Japan.
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: I
authors.initials: I
authors.lastname: Nagano
authors.forename: A
authors.initials: A
authors.lastname: Matsuo
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D000947:Antigens, Helminth; D003433:Crosses, Genetic; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics; D007814:Larva / Q000235:genetics; D008297:Male; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D014234:Trichinella / Q000737:chemistry / Q000235:genetics*; D017160:Trichinella spiralis / Q000737:chemistry / Q000235:genetics*; D014235:Trichinellosis / Q000235:genetics / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D017931:DNA Primers; D018509:DNA, Helminth; D015801:Helminth Proteins; C097419:excretory-secretory antigen, Trichinella; D003576:Electron Transport Complex IV