title: Salmon antithrombin has only three carbohydrate side chains, and shows functional similarities to human beta-antithrombin.
abstract: Antithrombin, a major coagulation inhibitor in mammals, has for the first time been cDNA cloned from a fish species. The predicted mature liver antithrombin of Atlantic salmon (Salmo salar) consists of 430 amino acids and shows about 67% sequence identity to mammalian and chicken antithrombins. Due to a single nucleotide replacement, Asn135 of the antithrombin in higher vertebrates is substituted by Asp in the salmon homolog. Hence, in contrast to the vertebrate antithrombins known so far, salmon antithrombin lacks the potential glycosylation site located close to the heparin binding site. The existence of only three N-linked side chains is evidenced by the sequential removal of three carbohydrate chains from salmon antithrombin during timed-digestion with N-glycosidase F. The high heparin binding affinity of the salmon inhibitor, Kd of 2.2 and 48 nM at I = 0.15 and 0.3, respectively, is very similar to that of the minor human isoform beta-antithrombin, which is not glycosylated at Asn135. Furthermore, the invariant third-position Ser137 at this glycosylation site of mammalian and chicken antithrombins is substituted by Thr in the salmon, a replacement that has been shown to induce full glycosylation in human antithrombin. Thus a rapidly reacting pool of antithrombin may have evolved in two different ways: absence of a glycosylation site in lower vertebrates vs. incomplete glycosylation of a part of the circulating antithrombin in higher vertebrates. Salmon antithrombin appears to have three complex oligosaccharide side chains containing sialic acid terminally linked alpha(2-3) to galactose, while trace amounts of Galbeta(1-4)GlcNAc suggest microheterogeneity due to partial loss of sialic acid.
authors.affiliation: Institute of Aquaculture Research; Department of Chemistry and Biotechnology, Agricultural University of Norway, Aas, Norway.
authors.forename: O
authors.initials: O
authors.lastname: Andersen
authors.forename: R
authors.initials: R
authors.lastname: Flengsrud
authors.forename: K
authors.initials: K
authors.lastname: Norberg
authors.forename: R
authors.initials: R
authors.lastname: Salte
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000991:Antithrombins / Q000737:chemistry*; D001483:Base Sequence; D002241:Carbohydrates / Q000737:chemistry*; D003001:Cloning, Molecular; D006031:Glycosylation; D006493:Heparin / Q000378:metabolism; D006801:Humans; D007448:Invertebrates / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011499:Protein Processing, Post-Translational; D020317:Salmo salar / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015843:Serpins / Q000737:chemistry; D013045:Species Specificity; D014714:Vertebrates / Q000378:metabolism
chemical_list: D000991:Antithrombins; D002241:Carbohydrates; D015843:Serpins; D006493:Heparin