title: Uniform distribution of transcription complexes over the entire Leishmania donovani clpB (hsp 100) gene locus.
abstract: We have analyzed the RNA polymerase density on the Leishmania donovani clpB gene locus. Our results show an even distribution of RNA polymerase over the clpB locus indicating an undiscriminative transcription. We conclude that, unlike the hsp70 genes, the clpB gene is not transcribed individually, but rather as part of a larger, polycistronic transcription unit.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wiesgigl
authors.forename: J
authors.initials: J
authors.lastname: Clos
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D049071:Endopeptidase Clp; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D007893:Leishmania donovani / Q000235:genetics* / Q000648:ultrastructure; D046911:Macromolecular Substances; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D016053:RNA, Protozoan / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: D016054:DNA, Protozoan; D006360:Heat-Shock Proteins; D046911:Macromolecular Substances; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D012321:DNA-Directed RNA Polymerases; D049071:Endopeptidase Clp; C095049:ClpB protein, Leishmania