Internal transcribed spacer rDNA can be used to infer the phylogenetic relationships of species within the genus Nematodirus (Nematoda: molineoidea).
Sequences of the first internal transcribed spacer rDNA were characterised for four veterinary important species of gastrointestinal nematodes from the genus Nematodirus. The sequence data were combined with previously published data of the second internal transcribed spacer to determine whether these rDNA regions provided a suitable number of informative characters to determine the phylogenetic relationships of species within the genus. A total of 32 alignment positions of the first internal transcribed spacer data set and 33 characters from the second internal transcribed spacer data set were informative in phylogenetic analyses. Irrespective of whether the data from each spacer were analysed separately or combined, only one most parsimonious tree was produced, with the relationships of the four species fully resolved. In addition, several regions of conservatism in the first internal transcribed spacer sequence among the four Nematodirus species suggests that this rDNA region may also provide phylogenetic information for higher taxonomic levels within the Molineoidea.
Audebert
F
F
Muséum National d'Histoire Naturelle, Laboratoire de Biologie Parasitaire, 61 rue Buffon, 75231, Paris, France.
Durette-Desset
M C
MC
Chilton
N B
NB
D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry*; D008969:Molecular Sequence Data; D009348:Nematoda / Q000235:genetics*; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid
D004275:DNA, Ribosomal