title: Cloning of the bovine leukemia virus proteinase in Escherichia coli and comparison of its specificity to that of human T-cell leukemia virus proteinase.
abstract: The proteinase of bovine leukemia virus (BLV) was cloned into pMal-c2 vector with N-terminal or with N- as well as C-terminal flanking sequences, and expressed in fusion with maltose binding protein. The proteinase self-processed itself from the fusion protein during expression and formed inclusion bodies. The enzyme was purified from inclusion bodies by cation-exchange chromatography followed by gel filtration. Specificity of the enzyme was compared to that of human T-cell leukemia proteinase type 1. Although the two viruses belong to the same subfamily of retroviruses, the differences in their proteinase specificity, based on kinetics with oligopeptide substrates representing naturally occurring cleavage sites as well as on inhibition pattern, appear to be pronounced.
authors.affiliation: Department of Biochemistry and Molecular Biology, University Medical School of Debrecen, P.O. Box 6, H-4012, Debrecen, Hungary.
authors.forename: G
authors.initials: G
authors.lastname: Zahuczky
authors.forename: P
authors.initials: P
authors.lastname: Boross
authors.forename: P
authors.initials: P
authors.lastname: Bagossi
authors.forename: G
authors.initials: G
authors.lastname: Emri
authors.forename: T D
authors.initials: TD
authors.lastname: Copeland
authors.forename: S
authors.initials: S
authors.lastname: Oroszlan
authors.forename: J M
authors.initials: JM
authors.lastname: Louis
authors.forename: J
authors.initials: J
authors.lastname: Tözsér
mesh_terms: D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D001909:Leukemia Virus, Bovine / Q000201:enzymology*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity
chemical_list: D004791:Enzyme Inhibitors; D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C079711:HTLV-1 protease