Characterization of 3-epi-1alpha,25-dihydroxyvitamin D3 involved in 1alpha,25-dihydroxyvitamin D3 metabolic pathway in cultured cell lines.
Using six different cultured cell models representing osteoblast, intestine, kidney and keratinocyte, we have demonstrated that 1alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)2D3) is metabolized into 3-epi-1alpha,25(OH)2D3 in vitamin D-target cells. Although differences existed in the amount of 3-epi-1alpha,25(OH)2D3 formed with different cell types, it was apparent that 1alpha,25(OH)2D3 was subjected to metabolism both through the C24-oxidation and 3-epimerization pathways. Time course and dose response studies showed that the production of 3-epi-1alpha,25(OH)2D3 was enzymatic. It is interesting to note that this epimerization proceeded from 3beta towards 3alpha unidirectionally, and this conversion was not inhibited by ketoconazole. These data suggest that cytochrome P450 related enzymes including the 24-hydroxylase would not affect this reaction. The biological activity of 3-epi-1alpha,25(OH)2D3 was found to be lower than the native 1alpha,25(OH)2D3 in suppressing of proliferation of HL-60 cells, while the affinity of 3-epi-1alpha,25(OH)2D3 for vitamin D-binding protein was 2.5-fold higher than that of 1alpha,25(OH)2D3. The results indicate that 3-epimerization may change the pharmacokinetics and catabolism of 1alpha,25(OH)2D3 in vitamin D-target cells.
Masuda
S
S
Department of Hygienic Sciences, Kobe Pharmaceutical University, Japan.
Kamao
M
M
Schroeder
N J
NJ
Makin
H L
HL
Jones
G
G
Kremer
R
R
Rhim
J
J
Okano
T
T
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002117:Calcitriol / Q000031:analogs & derivatives / Q000378:metabolism* / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D018922:HL-60 Cells; D006801:Humans; D007654:Ketoconazole / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D009928:Organ Specificity; D051381:Rats; D018167:Receptors, Calcitriol / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013552:Swine; D014809:Vitamin D-Binding Protein / Q000378:metabolism
D000935:Antifungal Agents; D018167:Receptors, Calcitriol; D014809:Vitamin D-Binding Protein; D002117:Calcitriol; D007654:Ketoconazole