title: The IFN-alpha-inducing capacity of Sendai Virus in human leukocytes is independent from the hemagglutinating and hemolytic activities and from the viral proteins of the Sendai Virus preparations.
abstract: About 30 Sendai Virus (SV) preparations, examined for their capacity to induce natural human interferon alpha from fresh human leukocytes (Le-IFN-alpha) of healthy donors, were characterized for hemagglutinating (HA) and hemolytic (HemA) activities and for SDS-PAGE proteic pattern. The SV preparations were produced by a single passage or by serial propagations through eggs in different conditions of multiplicity of infection (m.o.i.). The produced SV subpopulations showed variable IFN-inducing capacity, the values of which are distributed over a 6-fold range resembling a Gaussian distribution. No detectable difference was observed comparing the SV preparation obtained by serial propagations with those obtained by a single passage. The variability of the measured HA activity and HemA activity and as well as the SDS-PAGE proteic pattern of the SV preparations did not correlate with the induced amount of IFN per cell. In the same experimental conditions to produce Le-IFN-alpha, u.v.-treated SV preparations were unable to induce interferon depending on the u.v.-treatment. So it can be concluded that the distinct nHu-IFN-alpha-inducing capacity of SV subpopulation could be mainly associated with divergent compositions of the viral RNAs rather than with a different contents of viral proteins, among those detectable in SDS-PAGE and those responsible for HA activity and for HemA activity.
authors.affiliation: Alfa Wassermann SpA--Biotechnology and Immunology Department, Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Mattana
authors.forename: L
authors.initials: L
authors.lastname: Scapol
authors.forename: S
authors.initials: S
authors.lastname: Silvestri
authors.forename: G C
authors.initials: GC
authors.lastname: Viscomi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006387:Hemagglutination, Viral / Q000187:drug effects / Q000276:immunology*; D006460:Hemolysin Proteins / Q000276:immunology / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis*; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D012367:RNA, Viral / Q000276:immunology / Q000528:radiation effects; D018108:Respirovirus / Q000254:growth & development / Q000276:immunology* / Q000528:radiation effects; D012692:Serial Passage; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D006460:Hemolysin Proteins; D016898:Interferon-alpha; D012367:RNA, Viral; D014764:Viral Proteins