Oxidative stress-related proteins A170 and heme oxygenase-1 are differently induced in the rat cerebellum under kainate-mediated excitotoxicity.
A170 and heme oxygenase-1 (HO-1) are characterized as oxidative stress-inducible proteins whose induction depends upon common transcription factor via antioxidant responsive element. We investigated the expression of A170 and HO-1 in the cerebellum after kainate administration. In situ hybridization showed constitutive expression of A170 and HO-1 mRNA in Purkinje cell layer; mild induction of A170 or HO-1 was detected, respectively, 8 or 24 h after kainate administration. Immunohistochemical studies also demonstrated that constitutive expression and the induction of A170 protein in Purkinje cells; the induction of HO-1 protein was detected in Bergmann glia but not in Purkinje cells. Thus, the transcription factors involved in the induction of A170 might be different from those in the induction of HO-1 under kainate-mediated excitotoxicity. The existence of cell type-specific stress response was suggested.
Nakaso
K
K
Department of Biochemistry, Faculty of Medicine, Tottori University, Yonago, Japan. kazuhiro@grape.med.tottori-u.ac.jp
Kitayama
M
M
Fukuda
H
H
Kimura
K
K
Yanagawa
T
T
Ishii
T
T
Nakashima
K
K
Yamada
K
K
D000818:Animals; D015152:Blotting, Northern; D002531:Cerebellum / Q000201:enzymology / Q000378:metabolism*; D004790:Enzyme Induction; D018690:Excitatory Amino Acid Agonists; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D051547:Heme Oxygenase-1; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007608:Kainic Acid; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D000071456:Sequestosome-1 Protein
D018690:Excitatory Amino Acid Agonists; D006360:Heat-Shock Proteins; D000071456:Sequestosome-1 Protein; C491079:Sqstm1 protein, rat; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; D007608:Kainic Acid