Long-range imino proton-13C J-couplings and the through-bond correlation of imino and non-exchangeable protons in unlabeled DNA.
Thanks to rather large (5-9 Hz) long-range imino proton-13C J-couplings, heteronuclear correlation experiments in H2O provide unambiguous assignment of imino protons by intranucleotide through-bond connectivities to guanosine H8 and thymidine CH3 protons, or sequence-specific assignment of non-exchangeable protons when the imino protons are identified independently. This method is demonstrated in the Dickerson dodecamer [d(CGCGAATTCGCG)]2 and in a human telomeric fragment of 22 nucleotides.
Phan
A T
AT
Groupe de Biophysique de l'Ecole Polytechnique et de l'UMR 7643 du CNRS, Palaiseau, France. pat@pmc.polytechnique.fr
D001483:Base Sequence; D002247:Carbon Isotopes; D004247:DNA / Q000737:chemistry*; D006151:Guanosine / Q000737:chemistry; D006801:Humans; D007098:Imino Acids / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011522:Protons; D016615:Telomere / Q000235:genetics; D013936:Thymidine / Q000737:chemistry; D013997:Time Factors; D014867:Water
D002247:Carbon Isotopes; D007098:Imino Acids; D011522:Protons; D014867:Water; D006151:Guanosine; D004247:DNA; D013936:Thymidine