title: Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay.
abstract: Glucocorticoids have profound effects on macrophage function and are widely used as anti-inflammatory drugs. Glucocorticoids receptor (GR) ligand binding capacity is a major determinant of cellular glucocorticoid sensitivity. The number and affinity of GR can be measured in a whole cell binding assay using (3)H-dexamethasone. Here, we describe a rapid and simple microplate assay for GR measurement using the human promonocytic cell line THP-1.
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Vrije University, Amsterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Jansen
authors.forename: B
authors.initials: B
authors.lastname: Uitdehaag
authors.forename: J W
authors.initials: JW
authors.lastname: Koper
authors.forename: T K
authors.initials: TK
authors.lastname: van Den Berg
mesh_terms: D002460:Cell Line; D003907:Dexamethasone / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D008024:Ligands; D009000:Monocytes / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
chemical_list: D008024:Ligands; D011965:Receptors, Glucocorticoid; C028697:dexamethasone receptor; D003907:Dexamethasone