title: The determination of busulphan in dissolution samples by flow injection analysis.
abstract: A robust colourimetric flow injection analysis (FIA) procedure is described for the determination of busulphan in dissolution samples of a 2 mg tablet formulation. The sample solution is injected directly into a reagent stream containing 4-(4-nitrobenzyl)pyridine/potassium hydrogen phthalate. An on-line heating stage allows the formation of a coloured pyrridinium salt species, which following stabilisation is detected spectrophotometrically at 570 nm. The method has been fully validated and is linear over the concentration range 0.004-0.024 mg of busulphan ml(-1). The method can also been applied to uniformity of content and bulk assay testing.
authors.affiliation: Pharmaceutical Development, GlaxoWellcome Research and Development, Hertfordshire, UK. AD47219@ggr.co.uk
authors.forename: A D
authors.initials: AD
authors.lastname: Dow
authors.forename: G
authors.initials: G
authors.lastname: Finlay
authors.forename: M S
authors.initials: MS
authors.lastname: Bloomfield
mesh_terms: D002066:Busulfan / Q000032:analysis*; D003124:Colorimetry; D005069:Evaluation Studies as Topic; D017022:Flow Injection Analysis / Q000379:methods; D011725:Pyridines / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013607:Tablets
chemical_list: D011725:Pyridines; D013607:Tablets; C008633:4-(4-nitrobenzyl)pyridine; D002066:Busulfan