Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects.
OBJECTIVE
To determine whether changes in subcutaneous adipose tissue plasminogen activator inhibitor-1 (PAI-1) expression influence plasma PAI-1 level during weight loss in obese humans.


METHODS
Study of the variations of PAI-1 levels both in plasma and in subcutaneous abdominal adipose tissue in 15 volunteer non-diabetic obese subjects, body mass index (BMI) 40.4.+/-1.9 kg/m2, aged 48+/-3 y, before and after a 3 week very low calorie diet (VLCD) programme (3.9+/-0.1 MJ/day).


METHODS
Plasma and adipose tissue PAI-1 protein levels were measured by enzyme-linked immunosorbent assay and PAI-1 mRNA levels were quantified by quantitative RT-competitive PCR.


RESULTS
VLCD induced weight loss (5.8+/-0.8 kg) and decreased plasma PAI-1 concentration (-26% (P<0. 01)). Surprisingly, PAI-1 mRNA and protein abundance in subcutaneous adipose tissue increased by 87% (P<0.05) and by 44% (P<0.01), respectively.


CONCLUSIONS
These data indicate thus that changes in subcutaneous adipose tissue PAI-1 expression are not involved in the decrease of plasma PAI-1 levels during VLCD in obese subjects. International Journal of Obesity (2000)24, 70-74
Bastard
J P
JP
Service de Biochimie, Hôpital de la Salpêtrière, AP-HP, Paris. jean-philippe.bastard@psl.ap-hop-paris.fr
Vidal
H
H
Jardel
C
C
Bruckert
E
E
Robin
D
D
Vallier
P
P
Blondy
P
P
Turpin
G
G
Forest
C
C
Hainque
B
B
D000005:Abdomen; D000273:Adipose Tissue / Q000378:metabolism*; D004038:Diet, Reducing; D002149:Energy Intake / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy / Q000235:genetics / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D015431:Weight Loss / Q000502:physiology
D017395:Plasminogen Activator Inhibitor 1