Evaluation of mini-VIDAS rapid test for detection of Listeria monocytogenes from production lines of fresh to cold-smoked fish.
This study was conducted to evaluate the efficacy of the mini-VIDAS Listeria monocytogenes (LMO) system (BioMérieux Vitek, Inc., Missouri, USA) for detection of L. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VIDAS-LMO is a fully automated system that uses fluorescent ELFA (Enzyme Linked Fluorescent Assay) technology for detection of Listeria monocytogenes antigens in food. It can be a rapid screening method alternative to time consuming classical isolation and identification. Two hundred and ninety five samples were tested in mini-VIDAS-LMO and in parallel by the ISO 11290-1 traditional protocol. The mini-VIDAS-LMO detected 8 of the 11 confirmed positive samples and presented 11 false positive results. The specificity of the mini-VIDAS-LMO found in this experiment was 0.96 and the sensitivity 0.73.
Vaz-Velho
M
M
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal. manuela@esb.ucp.pt
Duarte
G
G
Gibbs
P
P
D000818:Animals; D015373:Bacterial Typing Techniques; D005399:Fishes / Q000382:microbiology*; D019084:Fluorescent Antibody Technique, Indirect; D005511:Food Handling; D005516:Food Microbiology; D007124:Immunoenzyme Techniques / Q000379:methods; D008089:Listeria monocytogenes / Q000145:classification / Q000302:isolation & purification*; D010473:Perciformes / Q000382:microbiology; D016929:Rhodococcus equi / Q000145:classification / Q000302:isolation & purification; D012474:Salmon / Q000382:microbiology; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification; D014337:Trout / Q000382:microbiology