title: Monkey pulpal response and microtensile bond strength beneath a one-application resin bonding system in vivo.
abstract: OBJECTIVE
  The aim of this in vivo study was to investigate the biocompatibility and microtensile bond strength of a one-application resin bonding system.
  
  
  METHODS
  Class V cavities were prepared on the facial surfaces of 36 intact monkey teeth, and the cavities were restored with an experimental one-application resin bonding system (TOF-1; Tokuyama Corp., Tokuyama, Japan) and a hybrid resin composite (PALFIQUE ESTELITE; Tokuyama Corp., Tokuyama, Japan). Histopathological changes of the restored teeth were evaluated at 3, 30 and 90 days after operation (N = 10). Microtensile bond tests were performed at 3 and 90 days after operation (N = 10).
  
  
  RESULTS
  Only two of 30 pulps showed a slight inflammatory cell infiltration. There were no statistically significant differences in the incidence of slight inflammatory cell infiltration among time periods. Bacterial penetration along the cavity walls could not be detected in any specimen. The mean microtensile bond strength at 3 days after operation was 20.6 MPa, and that at 90 days was 14.9 MPa. Differences in bond strengths between the 3 day specimen and the 90 day specimen were statistically significant (p < 0.05).
  
  
  CONCLUSIONS
  The one-application resin bonding system exhibited acceptable biologic compatibility to the monkey pulp. Although there were statistically significant differences in bond strengths between the 3 day specimen and the 90 day specimen, this material provided a hermetic seal, eliminating bacterial microleakage.
authors.affiliation: Department of Operative Dentistry, Faculty of Dentistry, Tokyo Medical and Dental University, Japan. y.kitasako.odnt@dent.tmd.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kitasako
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: P N
authors.initials: PN
authors.lastname: Pereira
authors.forename: M
authors.initials: M
authors.lastname: Okuda
authors.forename: H
authors.initials: H
authors.lastname: Sonoda
authors.forename: M
authors.initials: M
authors.lastname: Otsuki
authors.forename: J
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects* / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods* / Q000706:statistics & numerical data; D003737:Dental Cavity Preparation / Q000379:methods; D003782:Dental Pulp / Q000187:drug effects* / Q000473:pathology; D019232:Dental Restoration Failure; D000882:Haplorhini; D008422:Materials Testing / Q000379:methods / Q000706:statistics & numerical data; D011671:Pulpitis / Q000139:chemically induced / Q000473:pathology; D019279:Resin Cements / Q000009:adverse effects* / Q000737:chemistry; D018709:Statistics, Nonparametric; D013718:Tensile Strength; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D019279:Resin Cements