Characterization of a zebrafish/mouse somatic cell hybrid panel.
We have characterized a collection of zebrafish/mouse somatic cell hybrids with 211 genes and markers chosen from the 25 zebrafish linkage groups. Most of the zebrafish genome is represented in this collection with 88% of genes/markers present in at least one hybrid cell line. Although most hybrids contain chromosomal fragments, there are a few instances where a complete or nearly complete zebrafish chromosome has been maintained in a mouse background, based on multiple markers covering the entire chromosome. In addition to their use in mapping studies, this collection of somatic cell hybrids should constitute an important tool as a source of specific chromosome fragments and for assessing the function of genome regions.
Chevrette
M
M
Urology Division, Department of Surgery, McGill University, Montreal, Quebec, H3G 1A4, Canada.
Joly
L
L
Tellis
P
P
Knapik
E W
EW
Miles
J
J
Fishman
M
M
Ekker
M
M
D000818:Animals; D002460:Cell Line; D008040:Genetic Linkage; D005819:Genetic Markers; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D015027:Zebrafish / Q000235:genetics*
D005819:Genetic Markers