Induction of a protection against S. mansoni with a MAP containing epitopes of Sm37-GAPDH and Sm10-DLC. Effect of coadsorption with GM-CSF on alum.
Studies of anti-S. mansoni immunological responses in individuals living in endemic areas identified immunogens (Sm37-GAPDH and Sm10-DLC) with vaccine candidate properties. Analysis of the epitopes of these immunogens indicated that: (i) Sm37-5 is a major B-cell epitope of Sm37-GAPDH and the IgG antibody reactivity toward this determinant is associated with resistance to reinfection; (ii) Sm10-T is a T-cell epitope of the major T-cell immunogen Sm10-DLC. This led us to test a multiple antigen peptide (MAP) containing Sm37-5 and Sm10-T as an anti-schistosome vaccine. This MAP induced a significant protective immune response in mice when injected in Freund's adjuvant or coadsorbed with GM-CSF on aluminium hydroxide. In the latter case the physical link between the cytokine and the antigen via the coadsorption on alum was necessary to obtain a protective response. Results of the antibody response indicated that when the MAP and GM-CSF were coadsorbed on alum, the antibody response against the Sm10-T epitope located in the NH(2)-terminal position was significantly amplified up to 30% of the anti-Sm37-5 response.
Argiro
L
L
Laboratory of Immunology and Genetics of Parasitic Diseases INSERM U399, Faculté de Médecine, Université de la Méditerranée, 27 bd Jean Moulin, 13385, Marseille, France.
Henri
S
S
Dessein
H
H
Kouriba
B
B
Dessein
A J
AJ
Bourgois
A
A
D000534:Alum Compounds; D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology; D004398:Dyneins / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000008:administration & dosage / Q000235:genetics; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006801:Humans; D007163:Immunosorbent Techniques; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000235:genetics
D000534:Alum Compounds; D000947:Antigens, Helminth; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; C041524:aluminum sulfate; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D004398:Dyneins