Determination by high-performance liquid chromatography of ketoprofen in vitro in rat skin permeation samples.
A direct, simple and rapid high-performance liquid chromatographic method has been developed for the determination of ketoprofen with ibuprofen as internal standard. Samples were chromatographed on a 5 microm Kromasil 100 C18 column. The mobile phase was a mixture of acetonitrile-0.01 M KH2PO4 adjusted to pH 1.5 with orthophosphoric acid 85% (60:40, v/v). Detection was at 260 nm and the run time was 10 min. The detector response was found to be linear in the concentration range 0.02 to 40 microg/ml. This HPLC assay has been applied to measure the "in vitro" percutaneous penetration of ketoprofen through rat skin.
de Jalón
E G
EG
Centro Galénico, Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Navarra, Pamplona, Spain.
Josa
M
M
Campanero
M A
MA
Santoyo
S
S
Ygartua
P
P
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007660:Ketoprofen / Q000032:analysis* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012869:Skin Absorption
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007660:Ketoprofen