Effect of latrunculin-B on outflow facility in monkeys.
Latrunculin-B (LAT-B), a macrolide derived from the marine sponge Latrunculia magnifica, sequesters monomeric G-actin, leading to the disassembly of actin filaments in cultured cells. In this study, we determined the effect of LAT-B on outflow facility in living monkeys. Total outflow facility was measured by 2-level constant pressure perfusion of the anterior chamber (AC) before and immediately after AC exchange infusion or 2 hr after topical application of LAT-B or vehicle. Both AC exchange infusion and topical application of LAT-B dose- and time-dependently increased outflow facility by two- to four-fold. Those findings suggest that pharmacological disorganization of the actin cytoskeleton in the trabecular meshwork by specific actin inhibitors such as LAT-B may be a useful anti-glaucoma strategy.
Peterson
J A
JA
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, WI, 53792-3220, USA.
Tian
B
B
Geiger
B
B
Kaufman
P L
PL
D000818:Animals; D001082:Aqueous Humor / Q000502:physiology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008252:Macaca fascicularis; D008387:Marine Toxins / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology*; D053778:Thiazolidines; D014129:Trabecular Meshwork / Q000187:drug effects* / Q000502:physiology
D019086:Bridged Bicyclo Compounds, Heterocyclic; D008387:Marine Toxins; D013844:Thiazoles; D053778:Thiazolidines; C037068:latrunculin B