Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome.
Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.
Griffin
D K
DK
Department of Biological Sciences, Brunel University, Uxbridge, Middlesex, UK. Darren.Griffin@brunel.ac.uk
Haberman
F
F
Masabanda
J
J
O'Brien
P
P
Bagga
M
M
Sazanov
A
A
Smith
J
J
Burt
D W
DW
Ferguson-Smith
M
M
Wienberg
J
J
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D020223:Chromosome Painting / Q000379:methods*; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D015342:DNA Probes; D005434:Flow Cytometry; D005777:Geese / Q000235:genetics; D016678:Genome; D007621:Karyotyping / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D020161:Physical Chromosome Mapping / Q000379:methods*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides