title: Insulin-like growth factor I mediates high-fat diet-induced adipogenesis in Osborne-Mendel rats.
abstract: To determine if high-fat (HF) diet-induced changes in adipose tissue cellularity are associated with the presence of paracrine growth factor(s) that alter preadipocyte proliferation, Osborne-Mendel rats were fed either a HF (76% energy) or a low-fat (LF, 12% energy) diet for 85 days. HF-fed rats had greater (P < 0.05) fat pad size, total fat cell number, number of small (30-70 microm) and large (80-140 microm) adipocytes, and percentage of 100- to 140-microm adipocytes compared with LF-fed rats. Preadipocytes in primary cell culture treated with inguinal adipose tissue conditioned medium (ATCM) prepared from HF-fed rats had greater (P < 0.05) proliferation compared with cultures treated with ATCM from LF-fed rats. Proliferative capacity of ATCM prepared from HF-fed rats was attenuated after the stripping of the medium of insulin-like growth factor I using an immunomagnetic bead separation system. These data are consistent with the concept that insulin-like growth factor I is involved in the paracrine regulation of adipogenesis.
authors.affiliation: Department of Foods and Nutrition, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: B G
authors.initials: BG
authors.lastname: Marques
authors.forename: D B
authors.initials: DB
authors.lastname: Hausman
authors.forename: A M
authors.initials: AM
authors.lastname: Latimer
authors.forename: K M
authors.initials: KM
authors.lastname: Kras
authors.forename: B M
authors.initials: BM
authors.lastname: Grossman
authors.forename: R J
authors.initials: RJ
authors.lastname: Martin
mesh_terms: D000273:Adipose Tissue / Q000473:pathology / Q000502:physiology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004041:Dietary Fats / Q000008:administration & dosage*; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D051381:Rats
chemical_list: D017077:Culture Media, Conditioned; D004041:Dietary Fats; D007334:Insulin-Like Growth Factor I