title: Lung injury, inflammation, and inflammatory stimuli in rats exposed to ozone.
abstract: The effects of ozone (O3) on airway epithelia, inflammation, and expression of inflammatory stimuli were investigated to delineate the mechanisms of inflammatory reactions relevant to lung injury. Because the airway responses to O3 develop gradually, this investigation included a time-sequence analysis. Rats exposed for 3 h to 1 ppm O3 were studied at 4-h intervals up to 20 h postexposure. Bronchoalveolar lavage fluid (BAL) was analyzed for albumin as an indicator of increased permeability, polymorphonuclear leukocytes (PMNs) to assess the inflammatory status, macrophage inflammatory protein-2 (MIP-2, an inflammatory chemokine), and cell adhesion molecules for their role in inflammation and PMN functions. The time-related increase in albumin was matched by a similar significant increase for PMNs, MIP-2, and intercellular adhesion molecule-1 (ICAM-1). However, no marked change occurred for beta-2 integrin (CD-18) and leukotriene B4 (LTB4). The results establish a temporal correlation of epithelial permeability with changes in inflammatory activity and stimuli responsible for PMN recruitment in the lung. The observations of elevated MIP-2 and ICAM-1 levels are consistent with their role in injury and inflammation. An early expression of MIP-2 mRNA in BAL cells, that is, immediately post O3 exposure, and the peak increase in BAL MIP-2 levels 4 h later support the chemotactic role of MIP-2 in PMN recruitment at 4- and 12-h time points. The rapid drop in MIP-2 and ICAM-1 levels appears to signal the termination of inflammatory cell recruitment, which is accompanied by an onset of recovery.
authors.affiliation: Department of Occupational and Environmental Health Sciences, Wayne State University, Detroit, Michigan 48202, USA. ad6268@wayne.edu
authors.forename: D K
authors.initials: DK
authors.lastname: Bhalla
authors.forename: S K
authors.initials: SK
authors.lastname: Gupta
mesh_terms: D000397:Air Pollution; D000418:Albumins / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D018821:CD18 Antigens / Q000032:analysis; D017931:DNA Primers; D004781:Environmental Exposure; D004797:Enzyme-Linked Immunosorbent Assay; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007975:Leukotriene B4 / Q000032:analysis; D008168:Lung / Q000473:pathology*; D008171:Lung Diseases / Q000139:chemically induced* / Q000473:pathology; D019402:Macrophage Inflammatory Proteins / Q000032:analysis / Q000235:genetics; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010083:Oxidants, Photochemical / Q000633:toxicity*; D010126:Ozone / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors
chemical_list: D000418:Albumins; D018821:CD18 Antigens; D017931:DNA Primers; D019402:Macrophage Inflammatory Proteins; D010083:Oxidants, Photochemical; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D007975:Leukotriene B4; D010126:Ozone