Involvement of acyl coenzyme A oxidase isozymes in biotransformation of methyl ricinoleate into gamma-decalactone by Yarrowia lipolytica.
We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lactone production. Monodisrupted strains produced similar levels of lactone as the wild-type strain (50 mg/liter) except for Deltapox3, which produced 220 mg of gamma-decalactone per liter after 24 h. The Deltapox2 Deltapox3 double-disrupted strain, although slightly affected in growth, produced about 150 mg of lactone per liter, indicating that Aox2p was not essential for the biotransformation. The Deltapox2 Deltapox3 Deltapox5 triple-disrupted strain produced and consumed lactone very slowly. On the contrary, the Deltapox2 Deltapox3 Deltapox4 Deltapox5 multidisrupted strain did not grow or biotransform methyl ricinoleate into gamma-decalactone, demonstrating that Aox4p is essential for the biotransformation.
Waché
Y
Y
Laboratoire de Biotechnologie, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, 21000 Dijon, France. ywache@u-bourgogne.fr
Laroche
C
C
Bergmark
K
K
Møller-Andersen
C
C
Aguedo
M
M
Le Dall
M T
MT
Wang
H
H
Nicaud
J M
JM
Belin
J M
JM
D042962:Acyl-CoA Oxidase; D001711:Biotransformation; D007527:Isoenzymes / Q000378:metabolism; D007783:Lactones / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D012277:Ricinoleic Acids / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*
D007527:Isoenzymes; D007783:Lactones; D012277:Ricinoleic Acids; C109757:decan-4-olide; C005235:methyl ricinoleate; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase