title: A putative 12 transmembrane domain cotransporter expressed in thymic cortical epithelial cells.
abstract: We have isolated a full-length cDNA clone (thymic stromal origin (TSO)-1C12) from a SCID thymus library using a probe from a PCR-based subtractive library enriched for sequences from fetal thymic stromal cells. TSO-1C12 mRNA is expressed mainly in the thymic cortex and is highly enriched in SCID thymus. Expression per cell is highest during fetal thymus development and decreases after day 16. Antipeptide Abs immunoprecipitated a hydrophobic, plasma membrane glycoprotein (thymic stromal cotransporter, TSCOT) whose translated sequence has weak homology to bacterial antiporters and mammalian cation cotransporters with 12 transmembrane domains. TSCOT represents a new member of this superfamily that is highly expressed in thymic cortical epithelial cells.
authors.affiliation: Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda MD 20892, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Kim
authors.forename: F A
authors.initials: FA
authors.lastname: Flomerfelt
authors.forename: K N
authors.initials: KN
authors.lastname: Lee
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: R H
authors.initials: RH
authors.lastname: Schwartz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004847:Epithelial Cells / Q000378:metabolism* / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary / Q000235:genetics; D017154:Stromal Cells / Q000378:metabolism / Q000502:physiology; D027981:Symporters; D013950:Thymus Gland / Q000166:cytology / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D027981:Symporters; C498632:Tscot protein, mouse