Suppression of chimpanzee NORS in hamster/chimpanzee hybrid: report on cell line R48-26.
We present the first documented NOR suppression in a hybridoma other than man-mouse for the hamster-chimpanzee hybrid cell line R48-26. Alu PCR and chromosome painting showed that in this cell line chimpanzee chromosomes 13-15-23 are maintained. NORs on chimpanzee chromosomes 15-23, whose presence was directly verified by FISH with H 28s rDNA, resulted inactive while telomeric rDNA on hamster chromosomes resulted active even if hamster chromosomes presented extensive rearrangements. We observed an all or nothing model in accordance with a model of regulation by selective transcriptional factors. The rearrangements of hamster chromosomes have not involved the location of NORs because they maintain a telomeric position.
Cavagna
P
P
Istituto di Antropologia Università di Firenze.
Marzella
R
R
Rocchi
M
M
Chiarelli
B
B
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D020223:Chromosome Painting; D006224:Cricetinae; D003412:Cricetulus / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006822:Hybrid Cells / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D009697:Nucleolus Organizer Region / Q000235:genetics*; D002679:Pan troglodytes / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D016615:Telomere / Q000235:genetics; D014407:Tumor Cells, Cultured
D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S