In vitro response of human gingival epithelial S-G cells to resveratrol.
WST-1 (mitochondrial dehydrogenase activities). Arrest of cell growth, due to inhibition of DNA synthesis, may explain the leveling of toxicity between day 2 and 3 for a 3-day continuous exposure to resveratrol. Irreversible damage to cell proliferation was noted in S-G cells exposed to 75-150 microM resveratrol for 2 days and then subsequently maintained for another 3 days in resveratrol-free medium. The cytotoxicity of resveratrol was neither potentiated nor ameliorated in the presence of an hepatic S9 microsomal fraction. The cytotoxicity of hydrogen peroxide to S-G cells was lessened by N-acetyl-L-cysteine and quercetin, but not by resveratrol. For nitric oxide, only N-acetyl-L-cysteine reduced toxicity. The ability of resveratrol to function as an antioxidant was, therefore, not noted under these test conditions.
Babich
H
H
Stern College for Women, Yeshiva University, Department of Biology, 245 Lexington Avenue, New York, NY 10016, USA. babich@ymail.yu.edu
Reisbaum
A G
AG
Zuckerbraun
H L
HL
D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents / Q000378:metabolism; D003520:Cyclophosphamide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents / Q000493:pharmacokinetics; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009499:Neutral Red / Q000493:pharmacokinetics; D010078:Oxazines; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology; D014966:Xanthenes
D018906:Antineoplastic Agents, Alkylating; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D004396:Coloring Agents; D007202:Indicators and Reagents; D010078:Oxazines; D013267:Stilbenes; D014966:Xanthenes; C005843:resazurin; D009499:Neutral Red; D003520:Cyclophosphamide; D001973:Bromodeoxyuridine; D000077185:Resveratrol