title: Processing of carnitine octanoyltransferase pre-mRNAs by cis and trans-splicing.
abstract: Trans-splicing is a mechanism by which two pre-mRNAs are processed to produce a mature transcript that contains exons from both precursors. This process has been described mostly in trypanosoma, nematodes, plant/algal chloroplasts and plant mitochondria [Bonen et al. (1993) FASEB J. 7, 40-46]. Our studies clearly demonstrate that a trans-splicing reaction occurs in the processing of the carnitine octanoyltransferase (COT) gene in rat liver. Three different mature transcripts of COT have been found in vivo, the canonical cis-spliced mRNA and two trans-spliced transcripts, in which either exon 2 or exons 2 and 3 are repeated. Splicing experiments in vitro also indicate the capacity of exon 2 to act either as a donor or as an acceptor of splicing, allowing the trans-splicing reactions to occur.
authors.affiliation: Department of Biochemistry, School of Pharmacy, University of Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Caudevilla
authors.forename: D
authors.initials: D
authors.lastname: Serra
authors.forename: A
authors.initials: A
authors.lastname: Miliar
authors.forename: C
authors.initials: C
authors.lastname: Codony
authors.forename: G
authors.initials: G
authors.lastname: Asins
authors.forename: M
authors.initials: M
authors.lastname: Bach
authors.forename: F G
authors.initials: FG
authors.lastname: Hegardt
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002333:Carnitine Acyltransferases / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D005091:Exons; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000201:enzymology; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D012322:RNA Precursors; D012333:RNA, Messenger; D002333:Carnitine Acyltransferases; C030162:carnitine octanoyltransferase