Liver and kidney kynurenine aminotransferase activity in different strains of rats.
Variations in liver and kidney kynurenine aminotransferase (KAT) activity in Pittsburg-Yoshida, Brown-Norway, albino Wistar, Sprague-Dawley, Long Evans and heterozygous Gunn rats were studied. In liver, values of KAT specific activity, expressed as mumoles of kynurenic acid formed per hour per mg of protein, were different in the groups of Brown-Norway and Pittsburg-Yoshida rats versus Long Evans and Sprague-Dawley rats. The activity expressed as mumoles of kynurenic acid per g of fresh liver showed other differences, being significantly higher in Gunn with respect to other strains of rats and lower in Pittsburg-Yoshida and Brown-Norway rats. In addition, KAT activity was significantly lower in Pittsburg-Yoshida than in Brown-Norway rats. In kidney, the specific activity of kynurenine aminotransferase showed significant differences in the values of Sprague-Dawley and Long Evans rats versus the other strains. The activity expressed per g of fresh tissue was significantly higher in Wistar, Sprague-Dawley, Long Evans and Gunn than in Pittsburg-Yoshida and Brown-Norway rats. No significant differences were found in values between hyperlipidemic Pittsburg-Yoshida and their control Brown-Norway rats. These results high-light the importance of considering various rat strains when inbred animal experimental models are used.
Costa
C V
CV
Department of Pharmaceutical Sciences, University of Padova, Italy. costa@dsfarm.unipd.it
Ragazzi
E
E
Caparrotta
L
L
Bertazzo
A
A
Biasiolo
M
M
Allegri
G
G
D000818:Animals; D006579:Heterozygote; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008190:Lyases; D008297:Male; D051381:Rats; D011911:Rats, Gunn; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains / Q000378:metabolism*; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013045:Species Specificity; D000637:Transaminases / Q000378:metabolism*
D000637:Transaminases; C427605:glutamine - phenylpyruvate transaminase; C021481:kynurenine-oxoglutarate transaminase; D008190:Lyases