title: Polyclonal antibodies from patients immunized with a globo H-keyhole limpet hemocyanin vaccine: isolation, quantification, and characterization of immune responses by using totally synthetic immobilized tumor antigens.
abstract: We have previously reported on a carbohydrate-based vaccine program for immunotherapy in cancer patients. One such vaccine, based on the globo H antigen conjugated to the protein keyhole limpet hemocyanin (KLH), has been in clinical evaluation. Although this and other carbohydrate vaccines have been shown to induce antibody responses, there are currently no quantitative data on the antibody levels achieved in immunized patients by these or other anti-cancer vaccines. We report herein an efficient route to complex synthetic oligosaccharides attached to an affinity matrix for identifying and isolating antibodies elicited against such a carbohydrate-based vaccine in humans. Pre- and postvaccination profiles from serum samples of patients immunized with globo H-KLH were compared. All anti-globo H antibody activity was efficiently separated from other serum constituents. The isolated antibodies were readily quantified, and their specificities were analyzed. Since no comparable data were available on antibodies resulting from the vaccination of other cancer patients, we compared the observed levels with those quoted in studies with bacterial polysaccharide vaccines that had been quantified. Remarkably, cancer patients immunized with globo H-KLH produce anti-globo H antibody levels often exceeding those formed by immunization with bacterial polysaccharides. In addition, substantial quantities of both IgG and IgM antibodies were elicited, clearly indicating a class switch to IgG. Taken together, these analyses serve to clarify several aspects of the immune response to the vaccine and give several new insights to the carbohydrate-based vaccination strategy. Furthermore, antibodies so isolated could well have applications in clinical therapy.
authors.affiliation: Division of Genitourinary Oncology, Department of Medicine, and Laboratory of Bio-organic Chemistry, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Wang
authors.forename: L J
authors.initials: LJ
authors.lastname: Williams
authors.forename: X F
authors.initials: XF
authors.lastname: Zhang
authors.forename: A
authors.initials: A
authors.lastname: Zatorski
authors.forename: V
authors.initials: V
authors.lastname: Kudryashov
authors.forename: G
authors.initials: G
authors.lastname: Ragupathi
authors.forename: M
authors.initials: M
authors.lastname: Spassova
authors.forename: W
authors.initials: W
authors.lastname: Bornmann
authors.forename: S F
authors.initials: SF
authors.lastname: Slovin
authors.forename: H I
authors.initials: HI
authors.lastname: Scher
authors.forename: P O
authors.initials: PO
authors.lastname: Livingston
authors.forename: K O
authors.initials: KO
authors.lastname: Lloyd
authors.forename: S J
authors.initials: SJ
authors.lastname: Danishefsky
mesh_terms: D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D019496:Cancer Vaccines / Q000276:immunology* / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007983:Lewis Blood Group Antigens / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000276:immunology; D018074:Vaccines, Conjugate / Q000276:immunology* / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000627:therapeutic use*
chemical_list: D000906:Antibodies; D019496:Cancer Vaccines; D007074:Immunoglobulin G; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D018074:Vaccines, Conjugate; D014614:Vaccines, Synthetic; C119370:globo H-keyhole limpet hemocyanin QS-21 vaccine