title: Tissue-specific expression of c-series gangliosides in the extraneural system.
abstract: c-Series gangliosides in extraneural tissues from young and adult rats were examined using thin-layer chromatographic (TLC) immunostaining with a specific monoclonal antibody A2B5. The composition of c-series gangliosides significantly differed among tissues. In adult rats, while liver tissue contained GT1c, GQ1c, and GP1c, renal tissue had GT3 as the major c-series ganglioside with GT2 in a lesser amount. Pancreatic tissue expressed c-series gangliosides that consisted of GT3, GT2, GQ1c, and GP1c. In other tissues including adrenal, thyroid, and eye lens, GT3 constituted the main c-series ganglioside species. While total ganglioside contents of extraneural tissues were much lower than that of brain tissue, the proportions of c-series gangliosides to total gangliosides were higher in many extraneural tissues. Interestingly, eye lens had the highest GT3 content among rat tissues examined. The compositions and concentrations of c-series gangliosides in liver and kidney significantly differed between 5-day-old and 7-week-old rats, suggesting the development-dependent expression of c-series gangliosides in these tissues. These results suggest that the expression of c-series gangliosides in extraneural tissues is regulated in a tissue-specific manner.
authors.affiliation: Department of Clinical Pharmacology and Therapeutics, School of Pharmaceutical Sciences University of Shizuoka, 52-1 Yada, Shizuoka, Japan. saitom@ys2.u-shizuoka-ken.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
mesh_terms: D000367:Age Factors; D000818:Animals; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis / Q000378:metabolism*; D015870:Gene Expression; D007159:Immunologic Tests; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005732:Gangliosides; C048178:pentasialoganglioside GP1c; C082014:tetrasialoganglioside GQ1c