Disruption of the Borrelia burgdorferi gac gene, encoding the naturally synthesized GyrA C-terminal domain.
The C-terminal domain of the A subunit of DNA gyrase, which we term Gac, is naturally synthesized in Borrelia burgdorferi as an abundant DNA-binding protein. Full-length GyrA, which includes the C-terminal domain, is also synthesized by the spirochete and functions as a subunit of DNA gyrase. We have disrupted synthesis of Gac as an independent protein and demonstrated that it is not essential for growth in a coumarin-resistant background. We detected no alterations in DNA maintenance, condensation, or topology in B. burgdorferi lacking this small DNA-binding protein.
Knight
S W
SW
Division of Biological Sciences, The University of Montana, Missoula, Montana 59812, USA.
Kimmel
B J
BJ
Eggers
C H
CH
Samuels
D S
DS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D003374:Coumarins / Q000494:pharmacology; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics
D001426:Bacterial Proteins; D003374:Coumarins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C030123:coumarin; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II