Apoptosis and expression of proliferative proteins in the developing nervous system and orofacial region of human embryos.
We have studied expression of PCNA and Ki-67 in the developing nervous system, sensory organs and orofacial regions in human embryos and fetuses, using monoclonal antibodies PC-10 and MIB-1 in three-step immunohistochemical method and apoptosis performed by TUNEL technique. Expression of PCNA and Ki-67 increased with the age. Apoptosis was rare in above mentioned regions.
Cernochová
D
D
Department of Histology and Embryology, Palacký University, Olomouc, Czech Republic.
Pospísilová
E
E
Nepozitková
D
D
Havelka
P
P
Lichnovský
V
V
D017209:Apoptosis; D002455:Cell Division; D005314:Embryonic and Fetal Development; D005147:Facial Bones / Q000196:embryology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D009420:Nervous System / Q000196:embryology* / Q000276:immunology / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D012160:Retina / Q000196:embryology / Q000276:immunology / Q000378:metabolism
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen