Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach.
We propose a rational approach to the generation of live viral vaccines: alteration of virally encoded type I IFN antagonists to attenuate virulence while retaining immunogenicity. We have explored this concept by using the influenza virus. Previously we have shown that the NS1 protein of influenza A virus possesses anti-IFN activity. We now present evidence that influenza A and B viruses encoding altered viral NS1 proteins are highly attenuated in the mouse host, yet provide protection from challenge with wild-type viruses.
Talon
J
J
Department of Microbiology, Mount Sinai School of Medicine, Box 1124, One Gustave L. Levy Place, New York, NY 10029, USA.
Salvatore
M
M
O'Neill
R E
RE
Nakaya
Y
Y
Zheng
H
H
Muster
T
T
García-Sastre
A
A
Palese
P
P
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D009981:Influenza B virus / Q000235:genetics* / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016297:Mutagenesis, Site-Directed; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000276:immunology
D017931:DNA Primers; C085820:INS1 protein, influenza virus; D007252:Influenza Vaccines; D017361:Viral Nonstructural Proteins