Translocation and coamplification of loci from chromosome arms 8p and 11q in the MDA-MB-175 mammary carcinoma cell line.
Rearrangement and coamplification of the 8p12 and 11q13 chromosomal regions occurs in a significant proportion of breast cancers. It usually involves a complex hybrid structure in which the FGFR1 and CCND1 genes are amplified. We report here a different type of 8p12-11q13 rearrangement in the MDA-MB-175 mammary carcinoma cell line. This amplification contains the NRG1/HGL (from 8p12-21) and DOC4 (from 11q13) genes, encoding respectively a ligand for ERBB receptors and a stress-induced protein which is a mammalian ortholog of Drosophila Tenm/Odz. It has been shown previously (Wang et al, Oncogene 18: 5718-5721, 1999) that these two genes are rearranged and fused by a translocation event. This type of event was not found in 30 tumors tested that showed coamplification of the 8p12 and 11q13 regions.
Adélaïde
J
J
Laboratoire de Biologie des Tumeurs, Institut Paoli-Calmettes (IPC), Marseille, France.
Chaffanet
M
M
Mozziconacci
M J
MJ
Popovici
C
C
Conte
N
N
Fernandez
F
F
Sobol
H
H
Jacquemier
J
J
Pébusque
M
M
Ron
D
D
Lafage-Pochitaloff
M
M
Birnbaum
D
D
D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008565:Membrane Proteins; D020890:Neuregulin-1 / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D008565:Membrane Proteins; D020890:Neuregulin-1; D009687:Nuclear Proteins; C113761:Tenm4 protein, mouse