Isolation and characterization of a human cDNA encoding a protein homologous to the 7.2-kDa protein (subunit X) of bovine ubiquinol-cytochrome C reductase.
Through large-scale sequencing of clones randomly selected from a library of human cDNAs, we have isolated a novel human gene termed hUQCR10. Its open reading frame encodes 63 amino acids that share 88.5% identity with the sequence of bovine ubiquinol-cytochrome C reductase 7.2-kDa protein (subunit X). A single 0.6-kb transcript was expressed in all human tissues examined, but was particularly abundant in heart and skeletal muscle, tissues that consume a large amount of oxygen. The gene product therefore may play a significant role in the cellular respiratory system. In support of this hypothesis, our immunohistochemical analysis revealed that the hUQCR10 protein is located in mitochondria. A homology search using computer programs determined the chromosomal localization of the gene at 22q12.
Akashi
H
H
Laboratory of Molecular Medicine, University of Tokyo, Japan.
Han
H J
HJ
Iizaka
M
M
Nakajima
Y
Y
Furukawa
Y
Y
Sugano
S
S
Imai
K
K
Nakamura
Y
Y
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003593:Cytoplasm / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D014450:Electron Transport Complex III / Q000235:genetics*; D006801:Humans; D008928:Mitochondria / Q000737:chemistry; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D014450:Electron Transport Complex III