Induction and repression of a Streptomyces lividans chitinase gene promoter in response to various carbon sources.
Induction and repression of a gene for chitinase (chiA) in Streptomyces lividans was investigated using a catechol 2,3-dioxygenase gene (xylE) as the reporter gene. Of various substrates examined, expression of the promoter (PchiA) was observed after a delay when colloidal chitin or small chitin-oligosaccharides were added to the medium. N-acetylglucosamine completely repressed the chiA promoter. The duration of the delay in expression of PchiA differed with the inducer used, with chitobiose inducing the activity most rapidly. The minimum concentration of chitobiose needed for induction was 1 microM. It appears, therefore, that an efficient inducer of the gene for chitinase in S. lividans is chitobiose.
Miyashita
K
K
National Institute of Agro-Environmental Sciences, Tsukuba, Japan. kmiyas@niaes.affrc.go.jp
Fujii
T
T
Saito
A
A
D050557:Catechol 2,3-Dioxygenase; D002688:Chitinases / Q000096:biosynthesis / Q000235:genetics*; D049308:Dioxygenases; D004187:Disaccharides / Q000494:pharmacology; D004790:Enzyme Induction; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D009844:Oligosaccharides / Q000494:pharmacology; D010105:Oxygenases / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D014312:Trisaccharides / Q000494:pharmacology
D004187:Disaccharides; D009844:Oligosaccharides; D014312:Trisaccharides; C041161:chitotriose; C012238:chitotetrose; C032438:chitobiose; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D002688:Chitinases