title: Removal of silver from photographic wastewater effluent using Acinetobacter baumannii BL54.
abstract: Acinetobacter baumannii BL54, a silver (Ag) resistant micro-organism was isolated from clinical samples collected at the Armed Forces Medical College hospital in Pune, India. The strain BL54 removed a high quantity of silver (2.85 mg/g biomass) from photographic wastewater effluent. Treatment of the cells with 10 mM EDTA or agitating the culture did not affect the removal process, while altering pH of the wastewater or pre-treating the cells with 0.5 mM 2,4-dinitrophenol (DNP), 20 microM N,N'-dicyclohexylcarbodiimide (DCC), 25 micrograms/mL cefotaxime, and polymyxin-B resulted in considerable decrease in removal of silver by the organism. Dead cells, or a Ags plasmid-cured derivative (BL54.1) removed little silver, which was mainly surface bound. The results, compared with accumulation of Ag by a sensitive culture of Escherichia coli K12 J53.2, suggest that A. baumannii BL54 has good potential for bioremediation of silver from photographic wastewater effluents.
authors.affiliation: Department of Genetics, Center for Research, Kerman University of Medical Sciences, Iran. shakibai@arg3.uk.ac.ir
authors.forename: M R
authors.initials: MR
authors.lastname: Shakibaie
authors.forename: B P
authors.initials: BP
authors.lastname: Kapadnis
authors.forename: P
authors.initials: P
authors.lastname: Dhakephalker
authors.forename: B A
authors.initials: BA
authors.lastname: Chopade
mesh_terms: D000150:Acinetobacter / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D010781:Photography; D012834:Silver / Q000378:metabolism* / Q000494:pharmacology; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D007220:Industrial Waste; D012834:Silver