Effects of Fusarium mycotoxins on levels of serotonin, melatonin, and 5-hydroxytryptophan in pineal cell cultures.
Analysis of melatonin (MEL) in pineal cell cultures by enzyme linked immunosorbent assay showed its concentration was increased by fusaric acid (FA), a mycotoxin produced by Fusarium species and associated with toxic duck and ostrich feeds. Subsequent cell culture studies demonstrated the precursors of MEL, 5-hydroxytryptophan (5HTP) and serotonin (5HT), were also affected by FA as well as other Fusarium mycotoxins. Herein we describe a technique for the analysis of 5HTP and 5HT in pineal cell cultures using HPLC with electrochemical detection (EC), and report on the effects of FA alone and in combination with fumonisin B1 (FB1) and deoxynivalenol (DON) on the levels of these MEL precursors.
Rimando
A M
AM
USDA ARS NPURU, University, MS 38677, USA. arimando@asrr.arsusda.gov
Porter
J K
JK
D006916:5-Hydroxytryptophan / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002264:Carboxylic Acids / Q000494:pharmacology; D002478:Cells, Cultured; D037341:Fumonisins; D005669:Fusaric Acid / Q000494:pharmacology*; D005670:Fusarium; D008550:Melatonin / Q000378:metabolism*; D009183:Mycotoxins / Q000494:pharmacology*; D010870:Pineal Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*; D014255:Trichothecenes / Q000494:pharmacology
D002264:Carboxylic Acids; D037341:Fumonisins; D009183:Mycotoxins; D014255:Trichothecenes; D012701:Serotonin; C056933:fumonisin B1; D006916:5-Hydroxytryptophan; D008550:Melatonin; C007262:deoxynivalenol; D005669:Fusaric Acid