Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants.
Transgenic plants expressing recombinant proteins from pathogenic microorganisms provide an inexpensive edible vaccine for induction of local immunity. Three transgenic plant lines were generated expressing the spike (S) protein of transmissible gastroenteritis virus (TGEV), a protein crucial for establishing mucosal immunity. All three of them were driven by a strong plant promoter. One construct contained the 3.7 kb 5' end of the native S gene sequence. In the second construct part of the S gene, from nucleotide 49 to 1785, was modified for optimal plant recognition and was fused to a plant signal peptide coding sequence. The third construct contained the D epitope-coding region of the S gene, from nucleotide 1201 to 1591, which was fused to the alfalfa beta-amylase gene. The S gene products were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting. Antigens from all three transgenic plant lines induced TGEV-specific immune responses in pigs as determined by virus neutralization and ELISA, and the resultant antibody titers for all three constructs were similar.
Tuboly
T
T
Department of Pathobiology, University of Guelph, Guelph, Canada.
Yu
W
W
Bailey
A
A
Degrandis
S
S
Du
S
S
Erickson
L
L
Nagy
E
E
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology / Q000517:prevention & control; D015870:Gene Expression; D005814:Genes, Viral; D005822:Genetic Vectors; D009500:Neutralization Tests; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013552:Swine; D014026:Nicotiana / Q000235:genetics; D005760:Transmissible gastroenteritis virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C064484:spike protein S, Transmissible gastroenteritis virus