Release of gram-negative outer-membrane proteins into human serum and septic rat blood and their interactions with immunoglobulin in antiserum to Escherichia coli J5.
Prior studies indicate that 3 bacterial outer-membrane proteins (OMPs) are released into serum associated with lipopolysaccharide (LPS) and are bound by IgG in antiserum to Escherichia coli J5 (anti-J5 IgG). The present studies analyzed the interaction of the OMPs with anti-J5 IgG and evaluated their release in an infected burn model of gram-negative sepsis. Affinity purification studies were performed on filtrates of bacteria incubated in human serum and plasma from rats with sepsis by use of O chain-specific anti-LPS IgG and anti-J5 IgG. All 3 OMPs were captured from septic rat blood by anti-LPS IgG. Release of OMPs into serum was highest for immature bacterial cultures and was increased by antibiotics in vitro and in vivo. Anti-J5 IgG selectively captured an 18-kDa OMP released into serum and into plasma from septic rats. The results raise the possibility that anti-J5 IgG may, in part, protect via anti-OMP antibodies.
Hellman
J
J
Infectious Disease Unit, Massachusetts General Hospital (CNY), Charlestown, MA, USA. Hellman@etherdome.mgh.harvard.edu
Loiselle
P M
PM
Zanzot
E M
EM
Allaire
J E
JE
Tehan
M M
MM
Boyle
L A
LA
Kurnick
J T
JT
Warren
H S
HS
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D011817:Rabbits; D051381:Rats; D018805:Sepsis / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D007074:Immunoglobulin G