title: SHV-13, a novel extended-spectrum beta-lactamase, in Klebsiella pneumoniae isolates from patients in an intensive care unit in Amsterdam.
abstract: Eleven clonally related Klebsiella pneumoniae isolates were examined. These had been isolated at an intensive care unit in Amsterdam in 1994. Their resistance was associated with a conjugative 170-kb plasmid which encoded a novel SHV beta-lactamase designated SHV-13. The SHV-13 enzyme had two substitutions compared with SHV-1: Leu35Gln and Gly238Ala. It hydrolyzed cefotaxime much more rapidly than ceftazidime or aztreonam.
authors.affiliation: Department of Medical Microbiology, St. Bartholomew's and The Royal London School of Medicine and Dentistry, London E1 2AD, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Yuan
authors.forename: L M
authors.initials: LM
authors.lastname: Hall
authors.forename: P H
authors.initials: PH
authors.lastname: Savelkoul
authors.forename: C M
authors.initials: CM
authors.lastname: Vandenbroucke-Grauls
authors.forename: D M
authors.initials: DM
authors.lastname: Livermore
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003428:Cross Infection / Q000201:enzymology* / Q000382:microbiology; D016172:DNA Fingerprinting; D007363:Intensive Care Units, Neonatal; D007700:Kinetics; D007710:Klebsiella Infections / Q000201:enzymology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009426:Netherlands; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C407007:beta-lactamase SHV-13; D001618:beta-Lactamases