Site-specific recombination in human cells catalyzed by phage lambda integrase mutants.
Phage lambda Integrase (Int) is the prototype of the so-called integrase family of conservative site-specific recombinases, which includes Cre and FLP. The natural function of Int is to execute integration and excision of the phage into and out of the Escherichia coli genome, respectively. In contrast to Cre and FLP, however, wild-type Int requires accessory proteins and DNA supercoiling of target sites to catalyze recombination. Here, we show that two mutant Int proteins, Int-h (E174 K) and its derivative Int-h/218 (E174 K/E218 K), which do not require accessory factors, are proficient to perform intramolecular integrative and excisive recombination in co-transfection assays inside human cells. Intramolecular integrative recombination is also detectable by Southern analysis in human reporter cell lines harboring target sites attB and attP as stable genomic sequences. Recombination by wild-type Int, however, is not detectable by this method. The latter result implies that eukaryotic co-factors, which could functionally replace the prokaryotic ones normally required for wild-type Int, are most likely not present in human cells.
Lorbach
E
E
Institute of Genetics, University of Cologne, Weyertal 121, Cologne, D-50931, Germany.
Christ
N
N
Schwikardi
M
M
Droge
P
P
D001287:Attachment Sites, Microbiological / Q000235:genetics; D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics; D015139:Blotting, Southern; D002384:Catalysis; D002460:Cell Line; D004278:DNA, Superhelical / Q000235:genetics; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics*; D011995:Recombination, Genetic / Q000235:genetics*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
D004278:DNA, Superhelical; D014764:Viral Proteins; D019426:Integrases