title: Water and monovalent ions in the minor groove of B-DNA oligonucleotides as seen by NMR.
abstract: During the past 8 years, two complementary nmr techniques-magnetic relaxation dispersion and nuclear Overhauser effect spectroscopy-have been applied extensively to the study of water and monovalent ions in the minor groove of B-DNA oligonucleotides in solution. In this review, the possibilities and limitations of the two methods are outlined, with emphasis on the interpretational steps whereby molecular-level information is extracted from the primary data. The results on sequence-dependent hydration and ion-DNA interactions obtained so far by these methods is summarized and critically assessed. The nmr results are also compared with structural data from x-ray crystallography.
authors.affiliation: Physical Chemistry 2, Lund University, P. O. Box 124, S-22100 Lund, Sweden. bertil.halle@fkem2.lth.se
authors.forename: B
authors.initials: B
authors.lastname: Halle
authors.forename: V P
authors.initials: VP
authors.lastname: Denisov
mesh_terms: D001483:Base Sequence; D002414:Cations, Monovalent / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000648:ultrastructure; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002414:Cations, Monovalent; D009838:Oligodeoxyribonucleotides; D014867:Water; D004247:DNA