[Leukocyte adhesion on a fibrinogen-coated surface under static conditions: experimentation and creation of a model].
The adhesion of polymorphonuclear leukocytes (PMNs) on the vascular endothelium is a complex process that occurs during different biological and pathological events and involves numerous molecules. The adhesion cascade is induced after PMN stimulation by various molecular or cellular signals. Fibrinogen is one of the substrates for CD11b/CD18 B2-integrins expressed at the PMN surface; fibrinogen-neutrophil binding is induced by inflammatory reactions. In order to understand this process, we have carried out studies on the basis of preliminary experiments on red blood cells and synthetic particles. The modelization of quiescent PMNs adhesion on a fibrinogen substrate was investigated with a sedimentation cell chamber. Two different physiological conditions were tested: the activated state of PMN by a synthetic pro-inflammatory activator (FMLP). The activated state of PMNs was both quantified by flow cytometry and controlled by fluorescence microscopy. The results suggest that quiescent neutrophils deposit in accordance with the ballistic deposition model. This random adsorption model differs from random sequential adsorption (RSA) in that the cells arriving at the surface are able to roll along cells previously adsorbed introducing the notion of gravitational attraction of cells. The preliminary results obtained with stimulated PMN do not allow to choose between one of this two deposition models. Nevertheless, the qualitative and quantitative effects of FMLP on neutrophils were demonstrated by modifications of adhesion molecules expression.
Labrador
V
V
Laboratoire d'Angiohématologie-Hémorhéologie, LEMTA UMR CNRS 7563, Faculté de Médecine, F-54500 Vandoeuvre-les-Nancy.
Legrand
S
S
Muller
S
S
Carl
P
P
Senger
B
B
Voegel
J C
JC
Latger-Cannard
V
V
Riha
P
P
Stoltz
J F
JF
D015703:Antigens, CD / Q000502:physiology; D018821:CD18 Antigens / Q000502:physiology; D002448:Cell Adhesion; D005340:Fibrinogen / Q000502:physiology*; D006454:Hemoglobins / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000502:physiology*; D016177:Macrophage-1 Antigen / Q000502:physiology; D008954:Models, Biological
D015703:Antigens, CD; D018821:CD18 Antigens; D006454:Hemoglobins; D016177:Macrophage-1 Antigen; D005340:Fibrinogen