Use of calibrated viral load standards for group M subtypes of human immunodeficiency virus type 1 to assess the performance of viral RNA quantitation tests.
Optimal management of human immunodeficiency virus type 1 (HIV-1) disease requires accurate quantitation of viral RNA concentrations in plasma. Evidence for increasing geographic intermixing of HIV-1 subtypes makes equivalent quantitation of all subtypes essential. The performances of six quantitative viral RNA tests are described, for the first time, with calibrated viral isolates of diverse subtypes.
Jagodzinski
L L
LL
Henry M. Jackson Foundation, Walter Reed Army Institute of Research, Rockville, Maryland 20850, USA. liagodzinski@hiv.hjf.org
Wiggins
D L
DL
McManis
J L
JL
Emery
S
S
Overbaugh
J
J
Robb
M
M
Bodrug
S
S
Michael
N L
NL
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000175:diagnosis*; D002138:Calibration; D005843:Geography; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D019562:Viral Load / Q000379:methods*
D012367:RNA, Viral