title: Effect of cisplatin on Allium cepa root meristem cells.
abstract: Allium cepa root growth was retarded by cisplatin treatment in a dose-dependent manner. A decrease in the mitotic index (MI) and an increase in the number of interphase cells was seen in cisplatin treated root tips. An increase in the frequency of abnormal mitoses and chromosomal aberrations was also observed in cisplatin treated groups which indicates its genotoxic effect on plant cells. The endogenous glutathione (GSH) level in the root tips decreased significantly after cisplatin treatment which may favour its increased interaction with cellular DNA thereby developing enhanced chromosomal aberrations and affecting cell divisions and root growth. It is suggested that the decrease in endogenous GSH may be related to the development of cisplatin-mediated genotoxic effects in plants.
authors.affiliation: Cell and Tumour Biology Laboratory, Department of Zoology, School of Life Sciences, North-Eastern Hill University, India.
authors.forename: D
authors.initials: D
authors.lastname: Khynriam
authors.forename: S B
authors.initials: SB
authors.lastname: Prasad
mesh_terms: D002945:Cisplatin / Q000494:pharmacology*; D018744:DNA, Plant / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D018519:Meristem / Q000187:drug effects / Q000254:growth & development; D019697:Onions; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development
chemical_list: D018744:DNA, Plant; D005978:Glutathione; D002945:Cisplatin