title: Surface hydrophobicity of the rat colonic mucosa is a defensive barrier against macromolecules and toxins.
abstract: BACKGROUND
  Mucosal surface hydrophobicity is a key factor of the gastric acid defence barrier. In the colon, surface hydrophobicity is high but its biological function remains unexplored.
  
  
  OBJECTIVE
  To investigate the functional changes of the barrier due to removal of the surface active phospholipid layer by a detergent, or to reinforcement of the surface active phospholipid by local application of a suspension of lipids.
  
  
  METHODS
  Surface hydrophobicity (contact angle measurement), colonic permeability (lumen to blood clearance of mannitol and dextran), and mucosal resistance against luminal aggression (distal colitis induced by dextran sodium sulphate, DSS) were investigated in three study groups: (a) rats pretreated with a detergent (Brij 35) known to remove surfactant lipids; (b) rats pretreated with a suspension of surface active lipids (tripalmitin and dipalmitoyl-phosphatidylcholine); and (c) control rats pretreated with the corresponding vehicles.
  
  
  RESULTS
  In controls, surface hydrophobicity was low on the caecal mucosa and high in colon and rectum. Detergent treatment reduced surface hydrophobicity, and increased colonic permeability to mannitol and dextran. Conversely, treatment with lipids increased surface hydrophobicity, and reduced colonic permeability. Administration of DSS induced a progressive loss of colonic surface hydrophobicity, and an increase in permeability to mannitol and dextran. Detergent treatment increased susceptibility to epithelial damage and mucosal inflammation by DSS. Treatment with lipids reduced susceptibility to DSS colitis.
  
  
  CONCLUSIONS
  Colonic surface hydrophobicity modulates permeability to hydrophilic molecules and protects against toxins.
authors.affiliation: Digestive System Research Unit, Hospital General Vall d'Hebron, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Lugea
authors.forename: A
authors.initials: A
authors.lastname: Salas
authors.forename: J
authors.initials: J
authors.lastname: Casalot
authors.forename: F
authors.initials: F
authors.lastname: Guarner
authors.forename: J R
authors.initials: JR
authors.lastname: Malagelada
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology*; D003106:Colon; D016264:Dextran Sulfate / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000502:physiology*; D008297:Male; D008353:Mannitol / Q000493:pharmacokinetics; D010743:Phospholipids / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010743:Phospholipids; D008353:Mannitol; D016264:Dextran Sulfate