Absence of enteroviral RNA in hearts explanted from patients with dilated cardiomyopathy.
BACKGROUND
The role of enterovirus infection in the pathogenesis of dilated cardiomyopathy (DCM) remains unclear. The objective of this study was to determine the prevalence of enterovirus in hearts explanted from patients with DCM and to compare it with enterovirus prevalence in hearts explanted from patients with other etiologies and in healthy donor hearts.


METHODS
A total of 138 cardiac samples were analyzed, 70 from heart donors and 68 from transplant recipients (22 with DCM). A highly sensitive enterovirus-specific nested RT-PCR was used to test for enterovirus.


RESULTS
All tests were negative except for one positive result that was attributed to carryover because sequencing of the amplification product showed it to be identical to the positive control.


CONCLUSIONS
In this study the sample of explanted hearts nested RT-PCR showed no evidence of the presence of enteroviral RNA. This suggests that if enterovirus had a role in the genesis of DCM, it does not require or lead to the persistence of the virus in myocardial tissue.
Crespo-Leiro
M G
MG
Heart Transplant Programme, Hospital Juan Canalejo and Instituto de Ciencias de la Salud, Universidad de La Coruña, Spain. MarisaCrespo@canalejo.cesga.es
Hermida-Prieto
M
M
Peña
F
F
Portela
F
F
Muñiz
J
J
Hermida
L F
LF
Juffe-Stein
A
A
Castro-Beiras
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D002311:Cardiomyopathy, Dilated / Q000821:virology*; D004770:Enterovirus / Q000302:isolation & purification*; D005260:Female; D006321:Heart / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification*
D012367:RNA, Viral