Dexamethasone represses 3,5,3'-triiodothyronine-stimulated expression of intercellular adhesion molecule-1 in the human cell line ECV 304.
The effect of the thyroid hormone L-3,5,3'-triiodothyronine (T3) on the expression of ICAM-1, a cell surface glycoprotein playing a pivotal role in inflammatory responses, was investigated. In ECV 304 cells, T3 (30 nmol/L) markedly increased ICAM-1 protein expression, with a peak after 24 h of treatment. ECV 304 human cells express both alpha1 and beta1 T3 receptors. In an attempt to understand the molecular mechanisms leading to the induction of the ICAM-1 gene by T3, we have studied the effects of a synthetic glucocorticoid, dexamethasone, and of a sesquiterpene lactone, parthenolide, on this T3-stimulated expression of ICAM-1. Our results demonstrate a repressive effect of dexamethasone and parthenolide on the expression of the protein ICAM-1 stimulated by T3. Both anti-inflammatory compounds interfere with this T3-mediated pathway in a dose-dependent manner.
Dietrich
J B
JB
Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, CNRS, Strasbourg, France. jbdietrich@neurochem.u-strasbg.fr
Zaepfel
M
M
Kuchler-Bopp
S
S
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007249:Inflammation; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000235:genetics*; D007700:Kinetics; D016253:Microscopy, Immunoelectron; D012717:Sesquiterpenes / Q000494:pharmacology; D014284:Triiodothyronine / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000911:Antibodies, Monoclonal; D012717:Sesquiterpenes; D014284:Triiodothyronine; D018799:Intercellular Adhesion Molecule-1; C002669:parthenolide; D003907:Dexamethasone