Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers.
We assessed Stanniocalcin-1 (STC-1) mRNA expression in human normal tissues, various types of human cancer cell lines, and cancer tissues obtained during surgery. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay developed to detect STC-1 mRNA, the transcripts were detected in 20 out of 21 cancer cell lines and in all tumor tissues from various types of cancer. Semi-quantitative analyses with multiplex RT-PCR showed that STC-1 mRNA tended to be enhanced in cancer tissues of hepatocellular carcinoma and colorectal cancer compared to background cancer-free tissues. Analysis of blood samples obtained from 11 patients with hepatocellular carcinoma before, after and during hepatectomy showed STC-1 mRNA expression in 8 out of 11 patients at least one time. Normal donor blood samples (n=31) were all-negative for STC-1 mRNA expression. Our results indicate that STC-1 mRNA might be a useful molecular marker for detection of tumor cells in blood from patients with various types of malignancies.
Fujiwara
Y
Y
Department of Surgery and Clinical Oncology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
Sugita
Y
Y
Nakamori
S
S
Miyamoto
A
A
Shiozaki
K
K
Nagano
H
H
Sakon
M
M
Monden
M
M
D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000097:blood; D006023:Glycoproteins / Q000235:genetics*; D006728:Hormones / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D012333:RNA, Messenger / Q000032:analysis* / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D006023:Glycoproteins; D006728:Hormones; D012333:RNA, Messenger; C051795:teleocalcin