title: Experimental inoculation with human granulocytic Ehrlichia agent derived from high- and low-passage cell culture in horses.
abstract: We report the successful infection throughout intravenous inoculation with low and high passage of in vitro-grown human granulocytic ehrlichiosis (HGE) agent in horses. Differences in disease severity but not in incubation time, hematological changes, PCR detection, ehrlichial load, seroconversion time, and titer range were noted between horses infected with a low and a high passage of in vitro-grown HGE agent.
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA. npusterla@ucdavis.edu
authors.forename: N
authors.initials: N
authors.lastname: Pusterla
authors.forename: J E
authors.initials: JE
authors.lastname: Madigan
authors.forename: K M
authors.initials: KM
authors.lastname: Asanovich
authors.forename: J S
authors.initials: JS
authors.lastname: Chae
authors.forename: E
authors.initials: E
authors.lastname: Derock
authors.forename: C M
authors.initials: CM
authors.lastname: Leutenegger
authors.forename: J B
authors.initials: JB
authors.lastname: Pusterla
authors.forename: H
authors.initials: H
authors.lastname: Lutz
authors.forename: J S
authors.initials: JS
authors.lastname: Dumler
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D004536:Ehrlichia / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D016873:Ehrlichiosis / Q000097:blood / Q000503:physiopathology / Q000662:veterinary*; D005260:Female; D006098:Granulocytes / Q000382:microbiology; D018922:HL-60 Cells; D006734:Horse Diseases / Q000382:microbiology / Q000503:physiopathology*; D006736:Horses; D006801:Humans; D008297:Male; D009919:Orchiectomy; D016133:Polymerase Chain Reaction