title: Cloning, sequencing, and expression of the pyruvate carboxylase gene in Lactococcus lactis subsp. lactis C2.
abstract: A functional pyc gene was isolated from Lactococcus lactis subsp. lactis C2 and was found to complement a Pyc defect in L. lactis KB4. The deduced lactococcal Pyc protein was highly homologous to Pyc sequences of other bacteria. The pyc gene was also detected in Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis bv. diacetylactis strains.
authors.affiliation: Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 55108, USA.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: D J
authors.initials: DJ
authors.lastname: O'Sullivan
authors.forename: K A
authors.initials: KA
authors.lastname: Baldwin
authors.forename: L L
authors.initials: LL
authors.lastname: McKay
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D011766:Pyruvate Carboxylase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D011766:Pyruvate Carboxylase