title: Use of experimental design to optimise a flow injection analysis assay for L-N-monomethylarginine.
abstract: A flow injection analysis (FIA) method to determine L-N-monomethylarginine, based on the reaction with ortho-phthalaldehyde in the presence of a suitable thiol-group, was optimised using experimental design. Two different approaches were followed wherein, (i) critical factors were identified in a screening design, and (ii) the simplex algorithm was used for further optimisation. In the first approach, the chemical reaction was optimised off-line and the optimal chemical conditions were transferred to the FIA-system. In the second approach the reaction and the FIA-system parameters were optimised together. The on-line approach is preferred.
authors.affiliation: ChemoAc, Vrije Universiteit Brussel, Pharmaceutical Institute, Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Vannecke
authors.forename: M S
authors.initials: MS
authors.lastname: Bloomfield
authors.forename: Y
authors.initials: Y
authors.lastname: Vander Heyden
authors.forename: D L
authors.initials: DL
authors.lastname: Massart
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry; D000465:Algorithms; D017022:Flow Injection Analysis / Q000379:methods; D015394:Molecular Structure; D012107:Research Design; D009764:o-Phthalaldehyde / Q000737:chemistry; D019323:omega-N-Methylarginine / Q000032:analysis* / Q000737:chemistry
chemical_list: D019323:omega-N-Methylarginine; D009764:o-Phthalaldehyde; D000111:Acetylcysteine