title: Lectin ligands on human dendritic cells and identification of a peanut agglutinin positive subset in blood.
abstract: As only a few cell surface markers for dendritic cells (DC) have been identified to date, this study examined the expression of ligands for lectin on different human DC populations. The ability of Concanavalin A (Con A), Wheat Germ Agglutinin (WGA), peanut agglutinin (PNA), and Helix pomatia (HPA) to bind to cell lines and PBMC and DC populations was analyzed by flow cytometry and specificity of binding confirmed using inhibitory and noninhibitory sugars. The cell lines showed non-lineage-restricted binding with Con A and WGA, independent of sialidase treatment. HPA and PNA bound to a restricted number of lines, but showed broad reactivity after sialidase treatment. The peripheral blood mononuclear cells (PBMC) and directly isolated blood DC, activated CD83(+) blood DC, epidermal Langerhans cells (LC), and monocyte-derived DC (Mo-DC) showed strong binding of Con A and WGA, both before and after sialidase treatment. No HPA binding ligands were detected on PBMC populations, including directly isolated blood DC. Following sialidase treatment CD3(+), CD16(+), and a subset of CD19(+) lymphocytes bound HPA. The lectin PNA bound weakly to CD14(+) monocytes and a subpopulation of circulating DC that were HLA-DR(hi)CDw123 Dr(hi)CDw123(dim)/(neg)CD11c(+). The HLA-DR(mod)CDw123(hi)CD11c(neg) subpopulation did not bind PNA. Without sialidase treatment LC expressed both HPA and PNA ligands, but these were either absent on activated CD83(+) blood DC or weakly expressed on Mo-DC. Following sialidase treatment PBMC populations, activated CD83(+) blood DC, and Mo-DC became PNA positive. Thus human DC express several lectin ligands and PNA binding identifies a subset of blood DC. That may reflect discrete changes associated with stages of DC development or functional maturation.
authors.affiliation: Haematology/Immunology Research Group, Christchurch Hospital, Christchurch, New Zealand.
authors.forename: H
authors.initials: H
authors.lastname: El Sherbini
authors.forename: B
authors.initials: B
authors.lastname: Hock
authors.forename: D
authors.initials: D
authors.lastname: Fearnley
authors.forename: A
authors.initials: A
authors.lastname: McLellan
authors.forename: S
authors.initials: S
authors.lastname: Vuckovic
authors.forename: D N
authors.initials: DN
authors.lastname: Hart
mesh_terms: D001665:Binding Sites; D015415:Biomarkers; D001773:Blood Cells / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006684:HLA-DR Antigens; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008024:Ligands; D019887:Peanut Agglutinin / Q000276:immunology*; D011485:Protein Binding
chemical_list: D015415:Biomarkers; D006684:HLA-DR Antigens; D008024:Ligands; D019887:Peanut Agglutinin