Modulation of calcium-induced clot formation of human plasma by Malassezia furfur.
The effect of Malassezia furfur on clot formation by human plasma was examined. The clotting time in the presence of M. furfur or Candida albicans was measured. M. furfur shortened the clotting time of calcium-induced clots by human plasma in a concentration-dependent manner. However, the protein content of the clots formed were not significantly different between the M. furfur-treated and the control group. The clotting time of clots triggered by thrombin or by placing plasma in glass tubes, which artificially activate the blood coagulation systems, were not affected by treatment with M. furfur. Moreover, acetone-treated M. furfur also shortens the calcium-induced clot formation time, while treatment with zymolyase, which causes decomposition of beta-glucan, did not shorten it. These results suggest that M. furfur activates the blood coagulation systems, and the beta-glucan portion of M. furfur plays a key role in shortening calcium-induced clot formation time.
Suzuki
T
T
Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, Japan.
Ohno
N
N
Ohshima
Y
Y
Yadomae
T
T
D000096:Acetone / Q000494:pharmacology; D001777:Blood Coagulation / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D002176:Candida albicans / Q000502:physiology; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D008290:Malassezia / Q000187:drug effects / Q000502:physiology*; D013917:Thrombin / Q000494:pharmacology; D013997:Time Factors
D000096:Acetone; C037833:zymolyase; D006867:Hydrolases; D013917:Thrombin; D002118:Calcium