Reductive cleavage of demeton-S-methyl by Corynebacterium glutamicum in cometabolism on more readily metabolizable substrates.
Corynebacterium glutamicum is able to biotransform demeton-S-methyl, an organophosphorus compound, during cometabolism with more readily metabolizable substrates. Among the cosubstrates used, fructose is the growth substrate that is most favorable for demeton-S-methyl biotransformation. The reaction mechanism of demeton-S-methyl biotransformation involves reductive cleavage of an S-C bond, which leads to accumulation of dimethyl thiophosphate in the culture medium.
Girbal
L
L
Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504 and UMR INRA 792, Centre de Bioingénierie Gilbert Durand, INSA, 31077 Toulouse cedex 4, France. girbal@insa-tsle.fr
Hilaire
D
D
Leduc
S
S
Delery
L
L
Rols
J L
JL
Lindley
N D
ND
D001673:Biodegradation, Environmental; D003352:Corynebacterium / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010755:Organophosphates / Q000378:metabolism; D063086:Organothiophosphates / Q000378:metabolism*; D010084:Oxidation-Reduction; D010759:Phosphorus Isotopes; D015262:Xenobiotics / Q000378:metabolism
D007306:Insecticides; D010755:Organophosphates; D063086:Organothiophosphates; D010759:Phosphorus Isotopes; D015262:Xenobiotics; D005632:Fructose; C030064:demeton-S-methyl