title: Telomere shortening is an in vivo marker of myocyte replication and aging.
abstract: To determine whether adult cardiac myocytes are capable of multiple divisions and whether this form of growth is restricted to a subpopulation of cells that retain this capacity with age, telomere lengths were measured in myocyte nuclei isolated from the left ventricle of fetal and neonatal Fischer 344 rats and rats at 4, 12, and 27 months after birth. Two independent methodologies were used for this analysis: laser scanning cytometer and confocal microscopy. In each case, fluorescence intensity of a peptide nucleic acid probe specific for telomeric sequence was evaluated. The two techniques yielded comparable results. Telomeric shortening increased with age in a subgroup of myocytes that constituted 16% of the entire cell population. In the remaining nondividing cells, progressive accumulation of a senescent associated nuclear protein, p16(INK4), was evidenced. In conclusion, a significant fraction of myocytes divides repeatedly from birth to senescence, counteracting the continuous death of cells in the aging mammalian rat heart.
authors.affiliation: Department of Medicine, New York Medical College, Valhalla, New York 10595, USA. jan_kajstura@nymc.edu
authors.forename: J
authors.initials: J
authors.lastname: Kajstura
authors.forename: B
authors.initials: B
authors.lastname: Pertoldi
authors.forename: A
authors.initials: A
authors.lastname: Leri
authors.forename: C A
authors.initials: CA
authors.lastname: Beltrami
authors.forename: A
authors.initials: A
authors.lastname: Deptala
authors.forename: Z
authors.initials: Z
authors.lastname: Darzynkiewicz
authors.forename: P
authors.initials: P
authors.lastname: Anversa
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D015415:Biomarkers; D002455:Cell Division / Q000502:physiology*; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis; D004247:DNA / Q000032:analysis; D005314:Embryonic and Fetal Development; D005333:Fetus / Q000196:embryology; D019084:Fluorescent Antibody Technique, Indirect; D006352:Heart Ventricles / Q000737:chemistry / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D019044:Image Cytometry / Q000379:methods; D007834:Lasers; D018613:Microscopy, Confocal / Q000379:methods; D009206:Myocardium / Q000737:chemistry / Q000166:cytology; D051381:Rats; D011916:Rats, Inbred F344; D016615:Telomere / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D015415:Biomarkers; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004247:DNA