title: Characterization of the selectivity filter of the epithelial sodium channel.
abstract: The epithelial sodium channel (ENaC) is composed of three homologous subunits termed alpha, beta, and gamma. Previous studies suggest that selected residues within a hydrophobic region immediately preceding the second membrane-spanning domain of each subunit contribute to the conducting pore of ENaC. We probed the pore of mouse ENaC by systematically mutating all 24 amino acids within this putative pore region of the alpha-subunit to cysteine and co-expressing these mutants with wild type beta- and gamma-subunits of mouse ENaC in Xenopus laevis oocytes. Functional characteristics of these mutants were examined by two-electrode voltage clamp and single channel recording techniques. Two distinct domains were identified based on the functional changes associated with point mutations. An amino-terminal domain (alpha-Val(569)-alpha-Gly(579)) showed minimal changes in cation selectivity or amiloride sensitivity following cysteine substitution. In contrast, cysteine substitutions within the carboxyl-terminal domain (alpha-Ser(580)-alpha-Ser(592)) resulted in significant changes in cation selectivity and moderately altered amiloride sensitivity. The mutant channels containing alphaG587C or alphaS589C were permeable to K(+), and mutation of a GSS tract (positions alpha587-alpha589) to GYG resulted in a moderately K(+)-selective channel. Our results suggest that the C-terminal portion of the pore region within the alpha-subunit contributes to the selectivity filter of ENaC.
authors.affiliation: Departments of Medicine and Physiology, School of Medicine, University of Pennsylvania and the Veteran Affairs Medical Center, Philadelphia, Pennsylvania 19104, USA.
authors.forename: S
authors.initials: S
authors.lastname: Sheng
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: K A
authors.initials: KA
authors.lastname: McNulty
authors.forename: D
authors.initials: D
authors.lastname: Avery
authors.forename: T R
authors.initials: TR
authors.lastname: Kleyman
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002414:Cations, Monovalent / Q000378:metabolism; D004252:DNA Mutational Analysis; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D053503:Epithelial Sodium Channels; D008094:Lithium / Q000378:metabolism; D051379:Mice; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D009865:Oocytes; D018408:Patch-Clamp Techniques; D017354:Point Mutation; D011188:Potassium / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*; D014981:Xenopus
chemical_list: D002414:Cations, Monovalent; D053503:Epithelial Sodium Channels; D011994:Recombinant Proteins; D015222:Sodium Channels; D000584:Amiloride; D008094:Lithium; D012964:Sodium; D011188:Potassium