title: Expression of CD27 on murine hematopoietic stem and progenitor cells.
abstract: Hematopoietic stem cells (HSC) are defined by self-renewal and multilineage differentiation potentials. In order to uncover the genetic program of HSC, we utilized high-density arrays to compare gene expression in highly purified mouse HSC and their mature progeny. One molecule specifically expressed in immature cells is CD27, a member of the TNF receptor family previously shown to play roles in lymphoid proliferation, differentiation, and apoptosis. We show here that the CD27 protein is expressed by about 90% of cells in a purified HSC population. Interestingly, the CD27pos cells are enriched for cells with short-term hematopoietic activities (colony forming potential in vivo and in vitro), while the minority CD27neg population is more effective in clonal long-term transplantation.
authors.affiliation: Department of Oncological Sciences, University of Utah, Salt Lake City 84132, USA.
authors.forename: A
authors.initials: A
authors.lastname: Wiesmann
authors.forename: R L
authors.initials: RL
authors.lastname: Phillips
authors.forename: M
authors.initials: M
authors.lastname: Mojica
authors.forename: L J
authors.initials: LJ
authors.lastname: Pierce
authors.forename: A E
authors.initials: AE
authors.lastname: Searles
authors.forename: G J
authors.initials: GJ
authors.lastname: Spangrude
authors.forename: I
authors.initials: I
authors.lastname: Lemischka
mesh_terms: D000818:Animals; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000096:biosynthesis*
chemical_list: D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7