title: A rapid method for PCR detection of bovine materials in animal feedstuffs.
abstract: Rapid identification of bovine materials in animal feedstuffs is essential for effective control of a potential source of bovine spongiform encephalopathy. We have developed a rapid method for the detection of the presence of bovine materials in animal feeds. Animal feed samples were prepared by a Chelex-100 treatment method, then subjected to polymerase chain reaction (PCR) detection. The assay can be completed in 2 h including 30 min for sample preparation, 35-65 min for PCR cycling and 30 min for gel electrophoresis. This method is not only rapid, simple and consistent, but also avoids a hazardous waste disposal issue associated with a previously described guanidine thiocyanate (GuSCN) extraction-PCR method.
authors.affiliation: Microbiology Division, National Center for Toxicological Research, Jefferson, AR 72079, USA. rwang@nctr.fda.gov
authors.forename: R F
authors.initials: RF
authors.lastname: Wang
authors.forename: M J
authors.initials: MJ
authors.lastname: Myers
authors.forename: W
authors.initials: W
authors.lastname: Campbell
authors.forename: W W
authors.initials: WW
authors.lastname: Cao
authors.forename: D
authors.initials: D
authors.lastname: Paine
authors.forename: C E
authors.initials: CE
authors.lastname: Cerniglia
mesh_terms: D000821:Animal Feed / Q000032:analysis / Q000592:standards*; D000818:Animals; D001688:Biological Products; D002417:Cattle / Q000235:genetics; D017931:DNA Primers; D016643:Encephalopathy, Bovine Spongiform / Q000517:prevention & control / Q000635:transmission; D008460:Meat / Q000032:analysis; D008903:Minerals / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D001688:Biological Products; D017931:DNA Primers; D008903:Minerals; C027762:bone meal