A new Mesorhizobium loti HAMBI 1129 phage isolated from Polish soil.
Phage A1 isolated from the rhizosphere of Lotus corniculatus was studied. It had a very narrow host range, as it was active only against Mesorhizobium loti HAMBI 1129. Phage A1 was classified as belonging to C Bradley's group bacteriophages. The latent period of A1 was 120-130 min and a burst size 13-17 particles per cell. The nature of the phage receptor was examined. Lipopolysaccharide from the phage-sensitive strain inactivated phage A1 in contrast to LPS from the phage-resistant bacteria. Purified LPS obtained from M. loti HAMBI 1129 had a high receptor activity with PhI(50) value of 0. 025 microgram/ml.
Turska-Szewczuk
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Department of General Microbiology, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, Poland.
Russa
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D020561:Alphaproteobacteria / Q000821:virology*; D001435:Bacteriophages / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology; D008854:Microscopy, Electron; D011991:Receptors, Virus / Q000378:metabolism; D012988:Soil Microbiology
D011991:Receptors, Virus