Quantification of anthranilic acid and its related enzyme activity in several different species.
Anthranilic acid (AA) has been attracted considerable attention as one of the L-tryptophan-kynurenine pathway metabolites in the central nervous system. In this study, the concentration of L-kynurenine (L-KYN) and AA in serum and CSF, and its related enzyme activities were determined in several species. In rabbits, CSF AA concentrations were lower and serum AA concentrations were slightly higher than those in other species. However, the concentrations of L-KYN were substantially higher in rabbits in both serum and CSF compared with other species. Tissue enzyme activities varied among species. In rabbits, lung IDO activities were higher, but liver kynurenine 3-hydroxylase activities were lower than those of the other species tested. Furthermore, brain kynurenine 3-hydroxylase activities were higher in gerbils than those in other species. These results clearly demonstrated that kynurenine pathway enzyme activities and metabolite concentrations vary with species.
Fujigaki
S
S
Department of Laboratory Medicine, Gifu University School of Medicine, Japan. fujigaki@cc.gifu-u.ac.jp
Saito
K
K
Fujii
H
H
Wada
H
H
Seishima
M
M
D000818:Animals; D001921:Brain / Q000201:enzymology; D005849:Gerbillinae; D006168:Guinea Pigs; D006867:Hydrolases / Q000378:metabolism; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007737:Kynurenine / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D050601:Kynurenine 3-Monooxygenase; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D006899:Mixed Function Oxygenases / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D014366:Tryptophan Oxygenase / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*
D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D007737:Kynurenine; D006899:Mixed Function Oxygenases; D014366:Tryptophan Oxygenase; D050601:Kynurenine 3-Monooxygenase; D006867:Hydrolases; C021480:kynureninase