Unique N-linked glycosylation of murine coronavirus MHV-2 membrane protein at the conserved O-linked glycosylation site.
The membrane (M) proteins of murine coronavirus (MHV) strains have been reported to contain only O-linked oligosaccharides. The predicted O-glycosylation site consisting of four amino acid residues of Ser-Ser-Thr-Thr is located immediately adjacent to the initiator Met and is well conserved among MHV strains investigated so far. We analyzed the nucleotide sequence of a highly virulent strain MHV-2 M-coding region and demonstrated that MHV-2 had a unique amino acid, Asn, at position 2 at the conserved O-glycosylation site. We also demonstrated that this substitution added N-linked glycans to MHV-2 M protein resulting in increment of molecular mass of MHV-2 M protein compared with JHM strain having only O-linked glycans.
Yamada
Y K
YK
Division of Experimental Animal Research, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo, Japan. ykyamada@nih.go.jp
Yabe
M
M
Ohtsuki
T
T
Taguchi
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000032:analysis; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D000086862:Coronavirus M Proteins; D017931:DNA Primers / Q000235:genetics; D005814:Genes, Viral; D006031:Glycosylation; D051379:Mice; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000737:chemistry / Q000235:genetics*; D011509:Proteoglycans / Q000235:genetics*; D016415:Sequence Alignment; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics*
D000086862:Coronavirus M Proteins; D017931:DNA Primers; C000706235:M protein, Murine hepatitis virus strain 2; D011509:Proteoglycans; D014763:Viral Matrix Proteins; D001216:Asparagine