Protection of pigs against classical swine fever with DNA-delivered gp55.
Classical swine fever virus causes significant mortality and morbidity in commercial piggeries in many countries in Europe and Asia. The protective antigen, gp55, is highly conformation-dependent and thus killed virus or bacterially produced proteins are not protective. This report demonstrates that DNA vaccination with the gene encoding gp55 can provide protective immunity with inoculation of two doses of 25 microg DNA or a single shot of 200 microg. Furthermore, the DNA can be delivered intramuscularly or by a simple spring-loaded needleless inoculator. In addition it is shown that inoculation of the DNA at a single site conveys the same level of immunity as division of the dose between two sites.
Andrew
M E
ME
CSIRO Animal Health, PO Bag 24, Geelong, Australia. marion.andrew@dah.csiro.au
Morrissy
C J
CJ
Lenghaus
C
C
Oke
P G
PG
Sproat
K W
KW
Hodgson
A L
AL
Johnson
M A
MA
Coupar
B E
BE
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D006691:Classical Swine Fever / Q000276:immunology / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004279:DNA, Viral / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D007273:Injections, Intramuscular; D009500:Neutralization Tests; D013552:Swine; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000627:therapeutic use; D014759:Viral Envelope Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology* / Q000627:therapeutic use; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics*
D000914:Antibodies, Viral; D004279:DNA, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus