The role of amino-acid residues in the hydrophobic patch surrounding the haem group of cytochrome f in the interaction with plastocyanin.
Soluble turnip cytochrome f has been purified from the periplasmic fraction of Escherichia coli expressing a truncated petA gene encoding the precursor protein lacking the C-terminal 33 amino-acid residues. The protein is identical [as judged by 1H-NMR spectroscopy, midpoint redox potential (+ 365 mV) and electron transfer reactions with plastocyanin] to cytochrome f purified from turnip leaves. Several residues in the hydrophobic patch surrounding the haem group have been changed by site-directed mutagenesis, and the proteins purified from E. coli. The Y1F and Q7N mutants showed only minor changes in the plastocyanin-binding constant Ka and the second-order rate constant for electron transfer to plastocyanin, whereas the Y160S mutant showed a 30% decrease in the overall rate of electron transfer caused in part by a 60% decrease in binding constant and partially compensated by an increased driving force due to a 27-mV decrease in redox potential. In contrast, the F4Y mutant showed increased rates of electron transfer which may be ascribed to an increased binding constant and a 14-mV decrease in midpoint redox potential. This indicates that subtle changes in the hydrophobic patch can influence rates of electron transfer to plastocyanin by changing the binding constants and altering the midpoint redox potential of the cytochrome haem group.
Gong
X S
XS
Department of Plant Sciences and Cambridge Centre for Molecular Recognition, University of Cambridge, UK.
Wen
J Q
JQ
Gray
J C
JC
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001665:Binding Sites; D001937:Brassica / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002942:Circular Dichroism; D003580:Cytochromes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D045348:Cytochromes f; D004579:Electron Transport; D004926:Escherichia coli; D006418:Heme / Q000737:chemistry; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010970:Plastocyanin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
D003580:Cytochromes; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D006418:Heme; D010970:Plastocyanin; D045348:Cytochromes f