title: Distinct recognition of collagen subtypes by alpha(1)beta(1) and alpha(2)beta(1) integrins. Alpha(1)beta(1) mediates cell adhesion to type XIII collagen.
abstract: Two integrin-type collagen receptors, alpha(1)beta(1) and alpha(2)beta(1), are structurally very similar. However, cells can concomitantly express the both receptors and they might have independent functions. Here, Chinese hamster ovary (CHO) cells, which lack endogenous collagen receptors, were transfected with either alpha(1) or alpha(2) integrin cDNA. Cells were allowed to adhere to various collagen types and their integrin function was tested by observing the progression of cell spreading. The cells expressing alpha(1)beta(1) integrin could spread on collagen types I, III, IV, and V but not on type II, while alpha(2)beta(1) integrin could mediate cell spreading on collagen types I-V. Type XIII is a transmembrane collagen and its interaction with the integrins has not been previously studied. CHO-alpha1beta1 cells could spread on human recombinant type XIII collagen, unlike CHO-alpha2beta1 cells. Integrins alpha(1)beta(1) and alpha(2)beta(1) recognize collagens with the specific alphaI domains. The alpha(1)I and alpha(2)I domains were produced as recombinant proteins, labeled with europium and used in a sensitive solid-phase binding assay based on time-resolved fluorescence. alpha(1)I domain, unlike the alpha(2)I domain, could attach to type XIII collagen. The results indicate, that alpha(1)beta(1) and alpha(2)beta(1) have different ligand binding specificity. Distinct recognition of different collagen subtypes by the alphaI domains can partially explain the differences seen in cell spreading. However, despite the fact that CHO-alpha1beta1 cells could not spread on type II collagen alpha(1)I domain could bind to this collagen type. Thus, the cell spreading on collagens may also be regulated by factors other than the integrins.
authors.affiliation: Department of Biological and Environmental Science, University of Jyväskylä, FIN-40351 Jyväskylä, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Nykvist
authors.forename: H
authors.initials: H
authors.lastname: Tu
authors.forename: J
authors.initials: J
authors.lastname: Ivaska
authors.forename: J
authors.initials: J
authors.lastname: Käpylä
authors.forename: T
authors.initials: T
authors.lastname: Pihlajaniemi
authors.forename: J
authors.initials: J
authors.lastname: Heino
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology*; D048429:Cell Size; D003094:Collagen / Q000145:classification / Q000378:metabolism*; D006224:Cricetinae; D006801:Humans; D039222:Integrin alpha1beta1; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D038981:Receptors, Collagen; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D039222:Integrin alpha1beta1; D016023:Integrins; D008024:Ligands; D038981:Receptors, Collagen; D011994:Recombinant Proteins; D003094:Collagen