title: Vascular smooth muscle cell apoptosis in aneurysmal, occlusive, and normal human aortas.
abstract: OBJECTIVE
  Apoptosis is a physiologic mechanism of cell death that regulates mass and architecture in many tissues. Apoptosis has been described as a feature in human vascular atherosclerosis and large vessel structural integrity. We examined the extent of vascular smooth muscle cell (VSMC) apoptosis in aneurysmal, occlusive, and normal human aortic tissue.
  
  
  METHODS
  Tissue samples of aneurysmal, occlusive, and normal human infrarenal aorta were evaluated. DNA fragmentation detection methods, immunohistochemistry, and DNA electrophoresis determined VSMC density, VSMC apoptosis, and apoptosis markers. Apoptotic cells and VSMC nuclei were counted with the use of computer-generated image analysis. Aortic subtypes were compared statistically by analysis of variance.
  
  
  RESULTS
  Seventeen aneurysmal, ten occlusive, and five normal human aortas were evaluated. By alpha(1)-actin immunostaining, VSMC density was least in aneurysmal aortas (271.8 +/- 13.5 cells/high-power field [HPF]) compared with occlusive aorta (278.2 +/- 39.4 cells/HPF) and normal aortas (291.0 +/- 25.4 cells/HPF; P = not significant). Presence of apoptotic VSMCs was demonstrated by terminal deoxynucleotidyl transferase fragment end labeling and propidium iodide nuclear staining. VSMC apoptosis was greatest within aneurysmal aortas with 11.7 +/- 1.5 cells/HPF compared with occlusive aortas with 3.3 +/- 0. 8 cells/HPF (P <.05) and normal aortas with 3.75 +/- 4.6 cells/HPF (P <.05). Significant differences in apoptosis markers, p53 or bcl-2, could not be demonstrated by immunohistochemistry or DNA electrophoresis in aortic subtypes.
  
  
  CONCLUSIONS
  Apoptosis of VSMCs is increased and VSMC density is decreased within the medial layer of aneurysmal aortic tissue. Structural degeneration of aortic tissue at the cellular level contributes to aneurysmal formation.
authors.affiliation: Departments of Vascular Surgery and Transplant Surgery, University of Tennessee Medical Center, Knoxville, TN, USA.
authors.forename: V L
authors.initials: VL
authors.lastname: Rowe
authors.forename: S L
authors.initials: SL
authors.lastname: Stevens
authors.forename: T T
authors.initials: TT
authors.lastname: Reddick
authors.forename: M B
authors.initials: MB
authors.lastname: Freeman
authors.forename: R
authors.initials: R
authors.lastname: Donnell
authors.forename: R C
authors.initials: RC
authors.lastname: Carroll
authors.forename: M H
authors.initials: MH
authors.lastname: Goldman
mesh_terms: D000368:Aged; D001012:Aorta, Abdominal / Q000378:metabolism / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal / Q000473:pathology*; D017209:Apoptosis; D001157:Arterial Occlusive Diseases / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000473:pathology*