Active steroidogenesis in the normal rat skin.
Using the radiolabeled precursors of adrenal steroids (14)C-11-deoxycorticosterone (DOC) and (14)C-progesterone ((14)C-PROG) we demonstrate that rat skin can synthesize a number of steroids. TLC separation of labeled metabolites show that among the (14)C-steroid products, two co-migrate with corticosterone (B) and 11-dehydrocorticosterone (A) standards. Thus, normal rodent skin possesses steroidogenic activity that can be shown using progesterone or DOC as primary substrates.
Slominski
A
A
Department of Pathology, Medical Center, Loyola University, Maywood, IL 60153, USA. aslomin@wpo.it.luc.edu
Gomez-Sanchez
C E
CE
Foecking
M F
MF
Wortsman
J
J
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D003345:Corticosterone / Q000096:biosynthesis; D003900:Desoxycorticosterone / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D020303:Rats, Inbred Dahl; D012867:Skin / Q000378:metabolism*; D013256:Steroids / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D013256:Steroids; D003900:Desoxycorticosterone; D011374:Progesterone; D003345:Corticosterone