title: Natural killer cell and alphabeta and gammadelta lymphocyte traffic into the liver graft immediately after liver transplantation.
abstract: BACKGROUND
  The persistence and migration of donor leukocytes has been well established, but cellular kinetics immediately after revascularization and the potential relevance of these different lymphocyte populations to spontaneous tolerance remain unclear. During the early hours of revascularization, there is a transitory "congestion" of the liver graft, which is evidence of an early phase that we have termed "first cellular contact."
  
  
  METHODS
  We have carried out by flow cytometry a prospective comparative study of the peak kinetics of lymphocyte subpopulations contained in: (a) peripheral blood and liver grafts at the time of multi-organ extraction from 14 brain-dead donors, (b) recipient peripheral blood before transplantation, and (c) recipient peripheral blood and liver grafts after (t=2 h) declamping and vascularization of the liver graft.
  
  
  RESULTS
  Before transplantation, the liver grafts contained large numbers of natural killer (NK) and NK-like cells with early lymphocyte activation. Immediately after revascularization, there was an influx of recipient NK and NK-like cells into the liver.
  
  
  CONCLUSIONS
  NK and CD3+CD56+ (NK-like) cells flooding into the liver graft immediately after revascularization could rapidly destroy allogeneic cells. However, spontaneous tolerance and the persistence of donor lymphocytes after orthotopic liver transplant could be a result of donor TCRalphabeta NK1.1 liver graft lymphocytes, which may be involved in the destruction of CD8+ T lymphocytes that would have received the apoptosis signal, and to NK and NK-like cell inhibition via inhibitory NK receptors. The decrease in gammadelta T lymphocytes in the two compartments suggests a mechanism of recirculation and capture in other lymphoid organs.
authors.affiliation: Department of Digestive Surgery C, Montpellier University Hospital Center, France.
authors.forename: F
authors.initials: F
authors.lastname: Navarro
authors.forename: P
authors.initials: P
authors.lastname: Portalès
authors.forename: S
authors.initials: S
authors.lastname: Candon
authors.forename: F R
authors.initials: FR
authors.lastname: Pruvot
authors.forename: G
authors.initials: G
authors.lastname: Pageaux
authors.forename: J M
authors.initials: JM
authors.lastname: Fabre
authors.forename: J
authors.initials: J
authors.lastname: Domergue
authors.forename: J
authors.initials: J
authors.lastname: Clot
mesh_terms: D000328:Adult; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D006650:Histocompatibility Testing; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology; D016031:Liver Transplantation / Q000473:pathology*; D016131:Lymphocyte Subsets / Q000737:chemistry*; D008297:Male; D018919:Neovascularization, Physiologic; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D014019:Tissue Donors
chemical_list: D006680:HLA Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta