Synthesis of immunoreagents for detection of deoxypyrrololine, a cross-link of bone collagen.
Two immunogens (5,6) and two probes (fluorescent 7 and chemiluminescent 8) were prepared from benzyl ester (-)-10. These immunoreagents (5,6 and 7,8) are useful for detection of collagen cross-link (+)-deoxypyrrololine (Dpl, 4), and for development of assays for osteoporosis.
Adamczyk
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M
Department of Chemistry, Abbott Laboratories, Abbott Park, IL 60064-6016, USA.
Johnson
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Reddy
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D000596:Amino Acids / Q000032:analysis*; D003094:Collagen / Q000737:chemistry*; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D007093:Imidazoles / Q000032:analysis*; D007202:Indicators and Reagents / Q000138:chemical synthesis*; D008163:Luminescent Measurements; D015335:Molecular Probes; D010024:Osteoporosis / Q000175:diagnosis
D000596:Amino Acids; D007093:Imidazoles; D007202:Indicators and Reagents; D015335:Molecular Probes; C405607:deoxypyrrololine; D003094:Collagen