Protective immunity induced by a Trypanosoma cruzi soluble extract antigen in experimental Chagas' disease. Role of interferon gamma.
CBA/J mice can be protected against lethal infection with Trypanosoma cruzi by treatment using T. cruzi soluble extract antigen (TCSE). In vivo administration of TCSE (400 microg/mouse) into naive mice increased the cellular proliferative response to Con A and elevated the levels of IFN-gamma. The production of IFN-gamma was extremely important in controlling the replication of the parasite since the protective activity of TCSE was completely abrogated by in vivo treatment with an anti IFN-gamma neutralizing antibody. These results suggest that depending on the level, cytokine production results in the control of replication of the parasite in experimental Chagas' disease.
Garcia
C A
CA
Department of Microbiology and Immunology, University of Campinas--UNICAMP, SP, Brazil.
Oliveira
E C
EC
Sakurada
J K
JK
Santos
L M
LM
D000818:Animals; D019138:Antibodies, Blocking / Q000009:adverse effects / Q000494:pharmacology; D000953:Antigens, Protozoan / Q000276:immunology / Q000627:therapeutic use*; D002474:Cell-Free System / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control*; D003208:Concanavalin A / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D016052:Protozoan Vaccines / Q000037:antagonists & inhibitors / Q000276:immunology / Q000627:therapeutic use; D012995:Solubility; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000276:immunology*
D019138:Antibodies, Blocking; D000953:Antigens, Protozoan; D016052:Protozoan Vaccines; D003208:Concanavalin A; D007371:Interferon-gamma