Anti-herpes simplex virus component isolated from Maclura cochinchinensis.
The powerful anti-herpes simplex virus (HSV) activity of Maclura cochinchinensis in vitro prompted us to carry out biologically-guided separation of the active component(s). Ethyl acetate and methanol extracts exhibited anti-HSV-2 activity at EC50 values of 38.5 micrograms/ml and 50.8 micrograms/ml, respectively. Although petroleum ether extract was inactive, a chloroform extract was too toxic to the test cell culture to perform the test. Biologically-guided chromatographic separation of the ethyl acetate extract yielded compound A, identified as morin using a spectroscopic method. Morin exhibited anti-HSV-2 activity at an EC50 value of 53.5 micrograms/ml. Morin pentaacetate was synthetized, but was inactive. This result suggested that free hydroxyl groups are required for anti-HSV-activity, as demonstrated previously for the antiviral activity of other flavonoids.
Bunyapraphatsara
N
N
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. pynby@mahidol.ac.th
Dechsree
S
S
Yoosook
C
C
Herunsalee
A
A
Panpisutchai
Y
Y
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000254:growth & development; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry*; D014709:Vero Cells; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D010936:Plant Extracts