title: A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control.
abstract: Phospholipase C and two inositol polyphosphate (IP) kinases constitute a signaling pathway that regulates nuclear messenger RNA export through production of inositol hexakisphosphate (IP6). The inositol 1,4,5-trisphosphate kinase of this pathway in Saccharomyces cerevisiae, designated Ipk2, was found to be identical to Arg82, a regulator of the transcriptional complex ArgR-Mcm1. Synthesis of inositol 1,4,5,6-tetrakisphosphate, but not IP6, was required for gene regulation through ArgR-Mcm1. Thus, the phospholipase C pathway produces multiple IP messengers that modulate distinct nuclear processes. The results reveal a direct mechanism by which activation of IP signaling may control gene expression.
authors.affiliation: Departments of Pharmacology and Cancer Biology and of Biochemistry, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Odom
authors.forename: A
authors.initials: A
authors.lastname: Stahlberg
authors.forename: S R
authors.initials: SR
authors.lastname: Wente
authors.forename: J D
authors.initials: JD
authors.lastname: York
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D026341:Minichromosome Maintenance 1 Protein; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D007295:Inositol Phosphates; D026341:Minichromosome Maintenance 1 Protein; D014157:Transcription Factors; C081651:inositol-1,4,5,6-tetrakisphosphate; D010833:Phytic Acid; D015544:Inositol 1,4,5-Trisphosphate; D001120:Arginine; D017853:Phosphotransferases (Alcohol Group Acceptor); C051038:Inositol 1,4,5-trisphosphate 3-kinase; C072095:inositol 1,3,4,5,6-pentakisphosphate-1-3-phosphatase; D010744:Phosphoric Monoester Hydrolases; D010738:Type C Phospholipases