Complete sequence analysis of the A*1103 allele.
Here we report the full-length sequence of a novel A*11 variant. The variant was identified by ARMS-PCR and serology, the sequence was confirmed by cloning and subsequent sequencing. This variant, A*1103, found in a family of oriental origin, resembles the A*1101 sequence in exon 2 but differs in exon 3 with regard to codons 151 and 152. The polymorphism's result in two amino acid substitutions (one conserved (His->Arg), one introducing a negative charge (Ala->Glu)) located in the alpha2 helical region. The arginine at amino acid position 151 is rare amongst Alocus alleles and is besides A*1103 only observed in A*29 variants, the glutamine at amino acid position 152 is shared with A*0301, A*25, *26, *34 variants and the A*02 subtypes A*0203, *0213 and *0226. In fact, the amino acid motif comprising codons 151 and 152 is unique to A*1103 among Alocus alleles, but is common to C-locus alleles.
Tijssen
H J
HJ
Blood Transfusion Service, University Hospital Nijmegen, The Netherlands.
Sistermans
E A
EA
van den Beucken
M
M
Krausa
P
P
Joosten
I
I
D000483:Alleles; D019943:Amino Acid Substitution; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000032:analysis; D015234:HLA-A Antigens / Q000235:genetics*; D059870:HLA-A11 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D003062:Codon; D015234:HLA-A Antigens; D059870:HLA-A11 Antigen; D004247:DNA