title: Translocation and coamplification of loci from chromosome arms 8p and 11q in the MDA-MB-175 mammary carcinoma cell line.
abstract: Rearrangement and coamplification of the 8p12 and 11q13 chromosomal regions occurs in a significant proportion of breast cancers. It usually involves a complex hybrid structure in which the FGFR1 and CCND1 genes are amplified. We report here a different type of 8p12-11q13 rearrangement in the MDA-MB-175 mammary carcinoma cell line. This amplification contains the NRG1/HGL (from 8p12-21) and DOC4 (from 11q13) genes, encoding respectively a ligand for ERBB receptors and a stress-induced protein which is a mammalian ortholog of Drosophila Tenm/Odz. It has been shown previously (Wang et al, Oncogene 18: 5718-5721, 1999) that these two genes are rearranged and fused by a translocation event. This type of event was not found in 30 tumors tested that showed coamplification of the 8p12 and 11q13 regions.
authors.affiliation: Laboratoire de Biologie des Tumeurs, Institut Paoli-Calmettes (IPC), Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Adélaïde
authors.forename: M
authors.initials: M
authors.lastname: Chaffanet
authors.forename: M J
authors.initials: MJ
authors.lastname: Mozziconacci
authors.forename: C
authors.initials: C
authors.lastname: Popovici
authors.forename: N
authors.initials: N
authors.lastname: Conte
authors.forename: F
authors.initials: F
authors.lastname: Fernandez
authors.forename: H
authors.initials: H
authors.lastname: Sobol
authors.forename: J
authors.initials: J
authors.lastname: Jacquemier
authors.forename: M
authors.initials: M
authors.lastname: Pébusque
authors.forename: D
authors.initials: D
authors.lastname: Ron
authors.forename: M
authors.initials: M
authors.lastname: Lafage-Pochitaloff
authors.forename: D
authors.initials: D
authors.lastname: Birnbaum
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008565:Membrane Proteins; D020890:Neuregulin-1 / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D008565:Membrane Proteins; D020890:Neuregulin-1; D009687:Nuclear Proteins; C113761:Tenm4 protein, mouse