title: Stable expression of a new chimeric fluorescent reporter in the human malaria parasite Plasmodium falciparum.
abstract: Stable transfection of a new, chimeric reporter in the human malaria parasite Plasmodium falciparum confers green fluorescence and methotrexate resistance that can be quantitated by Western blotting and flow cytometry. This provides a sensitive, live reporter for exploitation of genomic and high-throughput assays for the identification of new pathogenic determinants.
authors.affiliation: Department of Pathology, Northwestern University School of Medicine, Chicago, Illinois 60611, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kadekoppala
authors.forename: K
authors.initials: K
authors.lastname: Kline
authors.forename: T
authors.initials: T
authors.lastname: Akompong
authors.forename: K
authors.initials: K
authors.lastname: Haldar
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004351:Drug Resistance; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D015870:Gene Expression; D017930:Genes, Reporter; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology; D008957:Models, Genetic; D010957:Plasmids; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D007166:Immunosuppressive Agents; D011993:Recombinant Fusion Proteins; D008727:Methotrexate