title: Immobilisation and evaluation of a vancomycin chiral stationary phase for capillary electrochromatography.
abstract: The macrocyclic antibiotic, vancomycin, is covalently bonded to LiChrospher diol silica packed columns and evaluated in capillary electrochromatography (CEC) both in the reversed-phase and polar organic mode. Initially, capillaries were packed with 5 microm LiChrospher 100 A diol silica and evaluated in CEC with a reversed-phase biphenyl-pyrene achiral test resulting in resolution and efficiency values of ca. 2.5 and 100000 plates meter(-1), respectively. Repeatability for this test (resolution and efficiency) was also examined and found to be acceptable for both run-to-run (n=5, 0.74% and 1.5%) and column-to-column (n=5, 3.4% and 9.0%), respectively. Similar results were obtained when the 10 microm LiChrospher 1000 A diol silica was examined with the exception of efficiency, where a reduced plate height value of four times lower was obtained compared to the 100 A material. A simple three step in-situ vancomycin immobilisation procedure was subsequently carried out on these packed diol columns. Selectivity was obtained for thalidomide enantiomers on this vancomycin chiral stationary phase in reversed-phase CEC with resolution and efficiency values of ca. 2.5 and 80000 plates meter(-1), with acceptable repeatability (n=8) 0.9% and 3.0%, respectively. Selectivity was also obtained for thalidomide enantiomers on this phase in the polar organic mode with resolution and efficiency values of ca. 2.5 and 120000 plates meter(-1), with acceptable repeatability (n=7) 0.9% and 2.0%, respectively. It was possible to deduce from a chemometric design carried out for evaluating the mobile phase component effects that organic modifier ratio, MeOH/MeCN, played a significant role in controlling both resolution and efficiency. It was also possible to separate a number of basic analytes including four beta-adrenergic blocking agents in the polar organic mode albeit with lower resolution and efficiency values, ca. 1.5 and 45000 plates meter(-1), respectively.
authors.affiliation: Analytical Chemistry, Pharmaceutical Research & Development, AstraZeneca R&D Mölndal, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Wikström
authors.forename: L A
authors.initials: LA
authors.lastname: Svensson
authors.forename: A
authors.initials: A
authors.lastname: Torstensson
authors.forename: P K
authors.initials: PK
authors.lastname: Owens
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D014640:Vancomycin