Sequential chromosome aberration analysis following radiotherapy - no evidence for enhanced genomic instability.
Chromosome analysis of peripheral blood lymphocytes using block staining was performed on 18 cancer patients who had received fractionated radiotherapy doses totalling 35-80 Gy. Samples were obtained from 13 individuals within 1 year of treatment and thereafter approximately annually up to a maximum of eight times (range: three to eight samples per individual). Sampling of the remaining five patients started later. Frequencies of cells with unstable chromosome aberrations showed a steady decline whereas frequencies of cells with just chromatid aberrations and gaps were initially low and remained so. There was no subsequent rise in any aberrant cell type in later years and thus no suggestion that the radiation exposure had induced a persistent or late manifesting state of genomic instability.
Tawn
E J
EJ
Genetics Unit, Westlakes Research Institute, Westlakes Science and Technology Park, Moor Row, Cumbria, UK. ejt@westlake.ac.uk
Whitehouse
C A
CA
Martin
F A
FA
D000328:Adult; D002869:Chromosome Aberrations; D005260:Female; D006689:Hodgkin Disease / Q000235:genetics / Q000532:radiotherapy; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000532:radiotherapy; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics / Q000532:radiotherapy; D011878:Radiotherapy / Q000009:adverse effects*; D013736:Testicular Neoplasms / Q000235:genetics / Q000532:radiotherapy; D013997:Time Factors