Expression of cyclooxygenase-1 and -2 in rheumatoid arthritis synovium.
The aim of this study was to investigate the expression and localization of cyclooxygenase-1 and -2 (COX-1 and COX-2) in synovial tissues from patients with rheumatoid arthritis (RA). Synovial tissues from 9 patients with RA and 5 patients with osteoarthritis (OA) were examined for COX-1 and COX-2 expressions by immunohistochemical staining using 2 polydonal COX-1 and COX-2 antibodies. In RA synovia, synovial lining cells showed intense immunostaining for COX-1, whereas slight to moderate staining was observed in inflammatory cells, stromal fibroblast-like cells and vascular endothelial cells. There was no significant difference in COX-1 expression between RA and OA synovia. The localization of COX-2 expression dearly differed from that of COX-1 expression, being most intense in inflammatory cells. However, there was no difference in COX-1 and COX-2 expressions between RA and OA synovial tissues. Our observations support that inflammatory mechanisms modulated by COX-1 and COX-2 in chronic RA synovium might be similar to those in chronic OA synovium.
Lee
Y H
YH
Department of Internal Medicine and Anatomical Pathology, College of Medicine, Korea University, Seoul.
Choi
S J
SJ
Kim
A
A
Kim
C H
CH
Ji
J D
JD
Song
G G
GG
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000201:enzymology* / Q000473:pathology; D002455:Cell Division; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D005260:Female; D005337:Fibrin / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D020556:Neutrophil Infiltration; D010003:Osteoarthritis / Q000201:enzymology; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000378:metabolism; D017154:Stromal Cells / Q000201:enzymology / Q000473:pathology; D013583:Synovial Membrane / Q000201:enzymology* / Q000473:pathology
D007527:Isoenzymes; D008565:Membrane Proteins; D005337:Fibrin; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases