Solution structure of the E. coli 70S ribosome at 11.5 A resolution.
Over 73,000 projections of the E. coli ribosome bound with formyl-methionyl initiator tRNAf(Met) were used to obtain an 11.5 A cryo-electron microscopy map of the complex. This map allows identification of RNA helices, peripheral proteins, and intersubunit bridges. Comparison of double-stranded RNA regions and positions of proteins identified in both cryo-EM and X-ray maps indicates good overall agreement but points to rearrangements of ribosomal components required for the subunit association. Fitting of known components of the 50S stalk base region into the map defines the architecture of the GTPase-associated center and reveals a major change in the orientation of the alpha-sarcin-ricin loop. Analysis of the bridging connections between the subunits provides insight into the dynamic signaling mechanism between the ribosomal subunits.
Gabashvili
I S
IS
Howard Hughes Medical Institute, Health Research, Inc., Albany, New York 11201-0509, USA.
Agrawal
R K
RK
Spahn
C M
CM
Grassucci
R A
RA
Svergun
D I
DI
Frank
J
J
Penczek
P
P
D001426:Bacterial Proteins / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D004926:Escherichia coli / Q000648:ultrastructure*; D020558:GTP Phosphohydrolases / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D020653:Peptide Elongation Factor G / Q000648:ultrastructure; D012329:RNA, Bacterial / Q000648:ultrastructure; D012335:RNA, Ribosomal / Q000648:ultrastructure; D012358:RNA, Transfer, Met / Q000648:ultrastructure; D012269:Ribosomal Proteins / Q000648:ultrastructure; D012270:Ribosomes / Q000648:ultrastructure*; D012996:Solutions
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D020653:Peptide Elongation Factor G; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012358:RNA, Transfer, Met; D012269:Ribosomal Proteins; D012996:Solutions; C024988:tRNA, formylmethionine-; D020558:GTP Phosphohydrolases