title: In vitro model for frontal sinus obliteration with bioactive glass S53P4.
abstract: An in vitro model was used to investigate the behavior of a massive frontal sinus obliteration with bioactive glass S53P4 (BG) for clinical purposes. Two sizes of granules (0.63-0.8 mm or 0.8-1.0 mm) in 16 separate BG amounts, weight 25 g, were tested both in simulated body fluid (SBF) and a buffer containing trishydroxymethyl aminomethane citric acid (TRIS-c.a) in standard conditions. The dissolution of silicon (Si) and phosphate (P) was detected with direct current plasma atom emission spectroscopy (DCP-AES) monthly up to 6 months. The BG masses were scanned by computer tomography (CT) and the scans analyzed by Region of Interest (ROI) technique. Calcium phosphate (CaP)- and silica (Si)-gel-layers were studied by scanning electron microscopy (SEM) at 1, 3, and 6 months. Cumulative loss of Si and P was stronger in TRIS -c.a than in SBF (p < 0.0001), and it was higher with smaller than with larger granules in both solutions (p < 0.0001). This was shown correspondingly by the decrease in Hounsfield units (HU) by ROI analysis (p < 0.0001). In SBF-soaked BG masses, the CaP-layer occurred on the uppermost granules, and in TRIS-c.a at 3-6 months, on the granules in the center and lower parts. The decrease of HU seems to reveal indirectly the resorption of BG.
authors.affiliation: Department of Otorhinolaryngology - Head and Neck Surgery, Turku University Central Hospital, Turku, Finland.
authors.forename: M J
authors.initials: MJ
authors.lastname: Peltola
authors.forename: J T
authors.initials: JT
authors.lastname: Suonpää
authors.forename: H
authors.initials: H
authors.lastname: Andersson
authors.forename: H S
authors.initials: HS
authors.lastname: Määttänen
authors.forename: K M
authors.initials: KM
authors.lastname: Aitasalo
authors.forename: A
authors.initials: A
authors.lastname: Yli-Urpo
authors.forename: P J
authors.initials: PJ
authors.lastname: Laippala
mesh_terms: D001826:Body Fluids; D018786:Bone Substitutes; D004577:Electron Probe Microanalysis; D005626:Frontal Sinus / Q000000981:diagnostic imaging / Q000601:surgery*; D015522:Frontal Sinusitis / Q000000981:diagnostic imaging / Q000601:surgery*; D005898:Glass; D006801:Humans; D007202:Indicators and Reagents; D008855:Microscopy, Electron, Scanning; D014057:Tomography, X-Ray Computed
chemical_list: D018786:Bone Substitutes; D007202:Indicators and Reagents; C403922:bioactive glass S53P4