title: Co-localization of FAM and AF-6, the mammalian homologues of Drosophila faf and canoe, in mouse eye development.
abstract: The Drosophila fat facets and canoe genes regulate non-neural cell fate decisions during ommatidium formation. We have shown previously that the FAM (fat facets in mouse) de-ubiquitinating enzyme regulates the function of AF-6, (mammalian canoe homologue), in the MDCK epithelial cell line (Taya et al., 1998. The Ras target AF-6 is a substrate of the fam de-ubiquitinating enzyme. J. Cell Biol. 142, 1053-1062). We report here that the expression of the FAM and AF-6 proteins overlaps extensively in the mouse eye from embryogenesis to maturity, especially in the non-neural epithelia including the retinal pigment epithelium, subcapsular epithelium of the lens and corneal epithelium. Expression is not limited to the epithelia however, as FAM and AF-6 also co-localize during lens fibre development as well as in sub-populations of the neural retina.
authors.affiliation: Centre for Molecular and Cellular Biology, The University of Queensland, Brisbane, QLD, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Kanai-Azuma
authors.forename: J S
authors.initials: JS
authors.lastname: Mattick
authors.forename: K
authors.initials: K
authors.lastname: Kaibuchi
authors.forename: S A
authors.initials: SA
authors.lastname: Wood
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004330:Drosophila; D029721:Drosophila Proteins; D010450:Endopeptidases / Q000032:analysis* / Q000276:immunology; D005123:Eye / Q000196:embryology*; D019476:Insect Proteins; D016547:Kinesins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009218:Myosins / Q000032:analysis* / Q000276:immunology; D043222:Ubiquitin Thiolesterase
chemical_list: C490657:Afdn protein, mouse; D029721:Drosophila Proteins; D019476:Insect Proteins; C092747:cno protein, Drosophila; D010450:Endopeptidases; D043222:Ubiquitin Thiolesterase; C404328:Usp9x protein, mouse; D009218:Myosins; D016547:Kinesins