Brachyury is a target gene of the Wnt/beta-catenin signaling pathway.
To identify target genes of the Wnt/beta-catenin signaling pathway in early mouse embryonic development we have established a co-culture system consisting of NIH3T3 fibroblasts expressing different Wnts as feeder layer cells and embryonic stem (ES) cells expressing a green fluorescent protein (GFP) reporter gene transcriptionally regulated by the TCF/beta-catenin complex. ES cells specifically respond to Wnt signal as monitored by GFP expression. In GFP-positive ES cells we observe expression of Brachyury. Two TCF binding sites located in a 500 bp Brachyury promoter fragment bind the LEF-1/beta-catenin complex and respond specifically to beta-catenin-dependent transactivation. From these results we conclude that Brachyury is a target gene for Wnt/beta-catenin signaling.
Arnold
S J
SJ
Max-Planck Institut für Immunbiologie, Stübeweg 51, D-79108, Freiburg, Germany.
Stappert
J
J
Bauer
A
A
Kispert
A
A
Herrmann
B G
BG
Kemler
R
R
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005326:Fetal Proteins; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D020825:T-Box Domain Proteins / Q000235:genetics*; D015534:Trans-Activators; D015533:Transcriptional Activation; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin; D000098908:Brachyury Protein
D003598:Cytoskeletal Proteins; D005326:Fetal Proteins; D011518:Proto-Oncogene Proteins; D020825:T-Box Domain Proteins; D015534:Trans-Activators; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin; D000098908:Brachyury Protein; C495267:CTNNB1 protein, mouse