A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper.
The hypersensitive resistance to tomato spotted wilt virus (TSWV) in pepper is determined by a single dominant gene (resistant allele: Tsw) in several Capsicum chinense genotypes. In order to facilitate the selection for this resistance, four RAPD (among 250 10-mer primers tested) were found linked to the Tsw locus using the bulked segregant analysis and 153 F2 individuals. A close RAPD marker was converted into a codominant cleaved amplified polymorphic sequence (CAPS) using specific PCR primers and restriction enzymes. This CAPS marker is tightly linked to Tsw (0.9 +/- 0.6 cM) and is helpful for marker-assisted selection in a wide range of genetic intercrosses.
Moury
B
B
Institut National de la Recherche Agronomique (INRA), Unité de génétique et amélioration des fruits et légumes, Montfavet, France.
Pflieger
S
S
Blattes
A
A
Lefebvre
V
V
Palloix
A
A
D000483:Alleles; D001947:Breeding; D002212:Capsicum / Q000235:genetics* / Q000821:virology; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D005799:Genes, Dominant; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D007113:Immunity, Innate; D010935:Plant Diseases / Q000235:genetics; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D017865:Tospovirus / Q000502:physiology*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers