BN52021 inhibits activation of peritoneal mast cells caused by hemorrhage and blood volume restoration.
An extensive blood loss activates generalized inflammatory response. Abdominal organs and especially intestines are very sensitive to the ischemia-reperfusion insults due to hemorrhagic shock (HS) and blood volume restoration. Previously obtained results suggest that studies on peritoneal lavage fluid (PLF) can contribute to elucidation of inflammatory processes in abdominal organs in HS. Histamine (H) levels, total cell, and mast cell (MC) numbers, and MC ultrastructure in the fluid lavaged from peritoneal cavity were compared in the following groups of rats: control (gr. 1), sham operation (gr. 2), untreated hemorrhagic shock (gr. 3), shock treated with blood volume restoration with lactated Ringer's solution (LR) (gr. 4), shock treated with platelet activating factor (PAF)-receptor antagonist Ginkgolide B (BN52021), and LR (gr. 5). A shock-related significant increase in total cell numbers, MC numbers, MC degranulation, and histamine levels in PLF were observed. The restoration of blood volume caused further elevation of the above phenomena (gr. 4) while BN52021 seemed to inhibit peritoneal MC mobilization and degranulation as well as to attenuate increase in peritoneal H level (gr. 5). The peritoneal cavity is a place of rapid and strong reaction to hemorrhage. Evaluation of peritoneal histamine levels might be helpful in the monitoring of shock dependent intra-abdominal processes. Peritoneal MC mobilization and degranulation, and increase in histamine level is inhibited by BN52021.
Debek
W
W
Department of Pediatric Surgery, Medical Academy, Białystok, Poland. debek_w@2n.pl
Chyczewski
L
L
Oldhafer
K J
KJ
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology* / Q000276:immunology; D001810:Blood Volume; D002452:Cell Count / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004224:Diterpenes; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects; D005260:Female; D046934:Ginkgolides; D006632:Histamine / Q000032:analysis; D007783:Lactones / Q000494:pharmacology* / Q000627:therapeutic use; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*
D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D006632:Histamine; C045856:ginkgolide B