title: Effect of stable fish oil on arterial thrombogenesis, platelet aggregation, and superoxide dismutase activity.
abstract: We examined the influence of dietary stable fish oil on aortic thrombosis, platelet aggregation, and superoxide dismutase (SOD) activity in a rat model. Twenty-nine Sprague-Dawley rats were fed regular chow supplemented with stable fish oil preparation (for 1 or 3 weeks), and 37 rats fed regular chow served as controls. The abdominal cavity was opened, and the abdominal aorta isolated. Whatman paper impregnated with 35% FeCl3 was wrapped around the surface of the aorta, and aortic flow was continuously recorded. In control rats, an occlusive platelet-fibrin-rich thrombus was formed in 21 +/- 3 min. Dietary fish oil in a time-dependent fashion delayed time to thrombus formation (24 +/- 2 min in rats fed fish oil for 1 week and 31 +/- 2 min in rats fed fish oil for 3 weeks), inhibited platelet aggregation (21 +/- 5% vs. 45 +/- 6%; p < 0.01) and increased SOD activity (p < 0.01). We conclude that dietary supplementation with stable fish oil delays formation of arterial thrombus, probably by reducing platelet aggregation and oxidative stress-associated arterial injury.
authors.affiliation: Department of Surgery, Forensic Medicine, University of Uppsala, Sweden.
authors.forename: L Y
authors.initials: LY
authors.lastname: Chen
authors.forename: R
authors.initials: R
authors.lastname: Jokela
authors.forename: D Y
authors.initials: DY
authors.lastname: Li
authors.forename: A A
authors.initials: AA
authors.lastname: Bavry
authors.forename: H
authors.initials: H
authors.lastname: Sandler
authors.forename: M
authors.initials: M
authors.lastname: Sjöquist
authors.forename: T
authors.initials: T
authors.lastname: Saldeen
authors.forename: J L
authors.initials: JL
authors.lastname: Mehta
authors.forename: A
authors.initials: A
authors.lastname: Bowry
mesh_terms: D000818:Animals; D004032:Diet; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism*; D013927:Thrombosis / Q000517:prevention & control*
chemical_list: D005395:Fish Oils; D013482:Superoxide Dismutase