Chiral separation and quantitation of pentazocine enantiomers in pharmaceuticals by capillary zone electrophoresis using maltodextrins.
The chiral separation of pentazocine was achieved by capillary electrophoresis using oligosaccharides. Enantiomers were separated on 100 mM Tris/H3PO4 buffer (pH 2.5) with 5% maltodextrin as a chiral selector, and migration behavior was monitored at 200 nm. Under these conditions, (-)- and (+)-pentazocine and dextromethorphan (internal standard) migrated within 9 min, and the resolution of pentazocine enantiomers was 2.54. Linear calibration curves were obtained in the range 5-50 microg/ml(-1) for each enantiomer. The detection limit of pentazocine enantiomers was 29 pg, and the recoveries of(-)- and (+)-pentazocine were 98.9 (R.S.D., 3.4%) and 101.4% (R.S.D., 4.3%) with 10 microg/ml(-1), respectively.
Watanabe
T
T
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan. watanabe@hoshi.ac.jp
Takahashi
K
K
Horiuchi
M
M
Kato
K
K
Nakazawa
H
H
Sugimoto
T
T
Kanazawa
H
H
D000701:Analgesics, Opioid / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010423:Pentazocine / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D013237:Stereoisomerism
D000701:Analgesics, Opioid; D004364:Pharmaceutical Preparations; D011134:Polysaccharides; C008315:maltodextrin; D010423:Pentazocine