SHV-13, a novel extended-spectrum beta-lactamase, in Klebsiella pneumoniae isolates from patients in an intensive care unit in Amsterdam.
Eleven clonally related Klebsiella pneumoniae isolates were examined. These had been isolated at an intensive care unit in Amsterdam in 1994. Their resistance was associated with a conjugative 170-kb plasmid which encoded a novel SHV beta-lactamase designated SHV-13. The SHV-13 enzyme had two substitutions compared with SHV-1: Leu35Gln and Gly238Ala. It hydrolyzed cefotaxime much more rapidly than ceftazidime or aztreonam.
Yuan
M
M
Department of Medical Microbiology, St. Bartholomew's and The Royal London School of Medicine and Dentistry, London E1 2AD, United Kingdom.
Hall
L M
LM
Savelkoul
P H
PH
Vandenbroucke-Grauls
C M
CM
Livermore
D M
DM
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003428:Cross Infection / Q000201:enzymology* / Q000382:microbiology; D016172:DNA Fingerprinting; D007363:Intensive Care Units, Neonatal; D007700:Kinetics; D007710:Klebsiella Infections / Q000201:enzymology* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009426:Netherlands; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C407007:beta-lactamase SHV-13; D001618:beta-Lactamases