Evidence for the transport of zinc(II) ions via the pit inorganic phosphate transport system in Escherichia coli.
A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10dCam) mutagenesis. The mutation was located within the pitA gene, which encodes the low-affinity inorganic phosphate transport system (Pit). The pitA mutant accumulated reduced amounts of zinc(II) when exposed to 0.5-2.0 mM ZnSO(4) during growth in Luria-Bertani medium.
Beard
S J
SJ
Division of Life Sciences, King's College London, Campden Hill Road, London, UK.
Hashim
R
R
Wu
G
G
Binet
M R
MR
Hughes
M N
MN
Poole
R K
RK
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017136:Ion Transport; D016254:Mutagenesis, Insertional; D010710:Phosphates / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology
D002352:Carrier Proteins; D004251:DNA Transposable Elements; D010710:Phosphates; D015032:Zinc