title: Characterization and functional role of leptin receptor in bovine adrenal medullary cells.
abstract: We report here the characterization and functional roles of the leptin receptor (ObR) in bovine adrenal medullary cells. The plasma membranes isolated from bovine adrenal medulla showed a single class of specific binding sites of (125)I-leptin with an apparent K(d) of 6.6 nM and B(max) of 62 fmol/mg protein. ObRa but not ObRb mRNA was detected in bovine adrenal medulla by reverse transcriptase-polymerase chain reaction. Incubation of cultured adrenal medullary cells with leptin (3-30 nM) for 20 min resulted in a significant increase in [(14)C]catecholamine synthesis from [(14)C]tyrosine without any change in catecholamine secretion. These findings suggest that leptin stimulates catecholamine synthesis through its receptors in bovine adrenal medullary cells.
authors.affiliation: Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan. yanagin@med.uoeh-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Yanagihara
authors.forename: K
authors.initials: K
authors.lastname: Utsunomiya
authors.forename: T B
authors.initials: TB
authors.lastname: Cheah
authors.forename: H
authors.initials: H
authors.lastname: Hirano
authors.forename: K
authors.initials: K
authors.lastname: Kajiwara
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: E
authors.initials: E
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Toyohira
authors.forename: Y
authors.initials: Y
authors.lastname: Uezono
authors.forename: S
authors.initials: S
authors.lastname: Ueno
authors.forename: F
authors.initials: F
authors.lastname: Izumi
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002395:Catecholamines / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D007457:Iodine Radioisotopes; D020738:Leptin / Q000378:metabolism*; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D002395:Catecholamines; D007457:Iodine Radioisotopes; D020738:Leptin; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin