Crystallization and preliminary X-ray diffraction studies of d(ACGTAGCTACGT)2:[actinomycin D, (echinomycin)2] and d(ACGTAGCTACGT)2:[actinomycin D, (triostin A)2] complexes.
A DNA-multiple drug complex, d(ACGTAGCTACGT)(2):[actinomycin D, (echinomycin)(2)] has been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 85.6, b = 72.8, c = 56.6 A, beta = 101.5 degrees at 93 K and Z = 8. The crystal diffracted to 3.0 A resolution along the DNA fiber axis and to 3.5 A resolution in other directions. The Patterson maps indicate that all complexes in the crystal are oriented along their helical axes in the [101;] direction.
Takusagawa
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HL
Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045--2106, USA. xraymain@ukans.edu
Takusagawa
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F
D003460:Crystallization; D018360:Crystallography, X-Ray; D003609:Dactinomycin / Q000737:chemistry* / Q000378:metabolism; D004448:Echinomycin / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D011810:Quinoxalines / Q000737:chemistry / Q000378:metabolism
D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D011810:Quinoxalines; C031945:triostin A; D003609:Dactinomycin; D004448:Echinomycin