Role of prostaglandin E2 and leukotriene B4 in skin reaction induced by transdermal application of propranolol.
Dermal application of propranolol (PRL) induced formation of erythema and edema, and pseudoeosinophil infiltration in epidermis and dermis at the application site in guinea pigs. We investigated the production of prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) at the application site of PRL and the role of these inflammatory chemical mediators in the occurrence of the skin reactions. PGE2 was found to be produced at the application site slightly after the accumulation of PRL released from the adhesive bandage in the patch test, and the amount of PGE2 increased continuously, with a peak value obtained at 24 h after application. The time-course changes resembled those of delta a* value, the index of erythema formation determined by colorimetric measurement, and edema formation. The production of PGE2 by dermal application of PRL was suppressed by local pretreatment with dexamethasone or indomethacin. However, no notable production of LTB4 was observed at the application site of PRL.
Kobayashi
I
I
Toxicological Research Center, Nitto Denko Corporation, Ibaraki, Osaka, Japan.
Hosaka
K
K
Maruo
H
H
Saeki
Y
Y
Kamiyama
M
M
Konno
C
C
Gemba
M
M
D000279:Administration, Cutaneous; D000287:Administration, Topical; D000319:Adrenergic beta-Antagonists / Q000008:administration & dosage / Q000009:adverse effects* / Q000493:pharmacokinetics; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D015232:Dinoprostone / Q000502:physiology*; D003875:Drug Eruptions / Q000473:pathology / Q000503:physiopathology*; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D006168:Guinea Pigs; D007202:Indicators and Reagents; D007975:Leukotriene B4 / Q000502:physiology*; D008297:Male; D011433:Propranolol / Q000008:administration & dosage / Q000009:adverse effects* / Q000493:pharmacokinetics; D012867:Skin / Q000473:pathology
D000319:Adrenergic beta-Antagonists; D000893:Anti-Inflammatory Agents; D007202:Indicators and Reagents; D007975:Leukotriene B4; D011433:Propranolol; D015232:Dinoprostone