Pseudo-symmetry characterization and refinement of a trigonal crystal form of naphthalene 1,2-dioxygenase.
Two trigonal crystal structures of naphthalene 1,2-dioxygenase from Pseudomonas sp. NCIB 9816-4 have been refined at 2.6 A resolution. The space group is R3, with four heterodimers in the asymmetric unit. The crystallographic threefold axis coincides with the symmetry axis of the active molecule, a mushroom-shaped alpha(3)beta(3) hexamer. The crystal is formed by symmetrical contacts between the hexamers on three different interaction surfaces, one on the beta-subunit and the other two on the alpha--subunits. Nickel ions mediate one of the alpha-subunit interactions. The two other types of packing contacts sustain two interlaced and almost independent crystal patterns with significantly different temperature factors. The space group of the individual crystal patterns is R32, with the corresponding twofold axes parallel to each other. The interactions between the crystal patterns separate the two parallel twofolds, eliminating the twofold symmetry for the whole crystal. The differences in temperature factors among the molecules in the asymmetric unit have been refined and are different for the two refined structures. An analysis of the structure factors of the pseudo-equivalent reflections showed that their differences lie in their phases and not in their amplitudes, suggesting that R(merge) is not an appropriate indicator for revealing the correct point group.
Carredano
E
E
Department of Molecular Biology, Swedish University of Agricultural Sciences, Box 590, Biomedical Center, S-751 24 Uppsala, Sweden.
Kauppi
B
B
Choudhury
D
D
Ramaswamy
S
S
D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D019281:Dimerization; D049308:Dioxygenases; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D010105:Oxygenases / Q000737:chemistry*; D011487:Protein Conformation; D011489:Protein Denaturation; D011549:Pseudomonas / Q000201:enzymology*; D013696:Temperature
D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D009532:Nickel; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase