The genetic analysis of F1 hybrid larvae between female Trichinella spiralis and male Trichinella britovi.
Hybrids between female Trichinella spiralis and male Trichinella britovi were constructed. Then, hybrid genotype was characterized by DNA markers including mitochondrial cytochrome c oxidase subunit I (CO I) gene, the gene encoding the 43-kDa excretory-secretory (ES) protein, and genomic DNA fragments specific for T. spiralis and T. britovi identified from random amplified polymorphism DNA (RAPD). PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial CO I gene revealed that all hybrids carried a T. spiralis pattern. The same analysis of the gene encoding the 43-kDa ES protein showed that each hybrid carried both T. spiralis and T. britovi gene type simultaneously. In the analysis of genomic DNA using RAPD-derived PCR primers, some hybrids carried T. spiralis and T. britovi-specific RAPD markers, while others carried the RAPD marker of T. spiralis only.
Wu
Z
Z
Department of Parasitology, Gifu University School of Medicine, Tsukasa 40, Gifu 500-8705, Japan.
Nagano
I
I
Matsuo
A
A
Takahashi
Y
Y
D000818:Animals; D000947:Antigens, Helminth; D003433:Crosses, Genetic; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D005260:Female; D005838:Genotype; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics; D007814:Larva / Q000235:genetics; D008297:Male; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D014234:Trichinella / Q000737:chemistry / Q000235:genetics*; D017160:Trichinella spiralis / Q000737:chemistry / Q000235:genetics*; D014235:Trichinellosis / Q000235:genetics / Q000469:parasitology
D000947:Antigens, Helminth; D017931:DNA Primers; D018509:DNA, Helminth; D015801:Helminth Proteins; C097419:excretory-secretory antigen, Trichinella; D003576:Electron Transport Complex IV