Validation of protein crystal structures.
Since the process of building and refining a model of a biomacromolecule based on crystallographic data is subjective, quality-control techniques are required to assess the validity of such models. During the 1990s, much experience was gained; the methods used and some of the lessons learned are reviewed here. In addition, an extensive compendium of quality criteria and quality-control methods that are or have been used to validate models of biomacromolecules has been compiled. The emphasis in this compendium is on the validation of protein crystal structures.
Kleywegt
G J
GJ
Department of Cell Biology, Uppsala University, Biomedical Centre, Box 596, SE-751 24 Uppsala, Sweden. gerard@xray.bmc.uu.se
D001671:Biochemistry / Q000193:education; D018360:Crystallography, X-Ray; D005069:Evaluation Studies as Topic; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015588:Observer Variation; D011487:Protein Conformation