The mechanisms of action of interleukin-1 on rabbit intestinal epithelial cells.
Interleukin-1 (IL-1) is an inflammatory mediator that increases Cl- secretion in intestinal epithelial cells. To identify the signal transduction pathway(s) involved in IL-1's action, cells were treated with IL-1 and the levels of cyclooxygenase (COX) enzymes, prostaglandin E2 (PGE2) and phospholipase A2-activating protein (PLAP), and the activity of phospholipase A2 (PLA2) were measured. IL-1 caused concentration- and time-dependent increases in the levels of PLA2 activity, and/or in the levels of PLAP, COX-2 and PGE2. The IL-induced increase in PGE2 levels was biphasic, with the first peak due to the increase in PLAP levels, and the second peak due to the increase in COX-2 levels. This increase in PGE2 levels may provide a mechanism for acute and chronic inflammation in the intestine.
Homaidan
F R
FR
Department of Physiology, Faculty of Medicine, American University of Beirut, Lebanon. fh01@aub.edu.1b
Zhao
L
L
Chakroun
I
I
Martin
C A
CA
Burakoff
R
R
D000818:Animals; D002478:Cells, Cultured; D003106:Colon; D051546:Cyclooxygenase 2; D003593:Cytoplasm / Q000201:enzymology; D015232:Dinoprostone / Q000378:metabolism; D004789:Enzyme Activation; D007375:Interleukin-1 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000502:physiology; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects
D007375:Interleukin-1; D007527:Isoenzymes; D011506:Proteins; D011994:Recombinant Proteins; C055324:phospholipase A2-activating protein; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2; D015232:Dinoprostone