title: Mechanistic studies on type I and type II dehydroquinase with (6R)- and (6S)-6-fluoro-3-dehydroquinic acids.
abstract: (6R)- and (6S)-6-Fluoro-3-dehydroquinic acids are shown to be substrates for type I and type II dehydroquinases. Their differential reactivity provides insight into details of the reaction mechanism and enables a novel enzyme-substrate imine to be trapped on the type I enzyme.
authors.affiliation: University Chemical Laboratory, Cambridge, UK.
authors.forename: E J
authors.initials: EJ
authors.lastname: Parker
authors.forename: C
authors.initials: C
authors.lastname: González Bello
authors.forename: J R
authors.initials: JR
authors.lastname: Coggins
authors.forename: A R
authors.initials: AR
authors.lastname: Hawkins
authors.forename: C
authors.initials: C
authors.lastname: Abell
mesh_terms: D003509:Cyclohexanecarboxylic Acids / Q000378:metabolism*; D003512:Cyclohexanones / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D013237:Stereoisomerism
chemical_list: D003509:Cyclohexanecarboxylic Acids; D003512:Cyclohexanones; D007527:Isoenzymes; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase