cAMP response element-binding protein-binding protein binds to human papillomavirus E2 protein and activates E2-dependent transcription.
cAMP response element-binding protein-binding protein (CBP) is a eucaryotic transcriptional co-activator that contains multiple protein-protein interaction domains for association with various transcription factors, components of the basal transcriptional apparatus, and other co-activator proteins. Here, we report that CBP is also a co-activator of the human papillomavirus (HPV) E2 protein, which is a sequence-specific transcription/replication factor. We provide biochemical, genetic, and functional evidence that CBP binds directly to HPV E2 in vivo and in vitro and activates E2-dependent transcription. Mutations in an amphipathic helix within HPV-18 E2 abolish its transcriptional activation properties and its ability to bind to CBP. Furthermore, the binding of CBP to E2 was shown to be necessary for E2-dependent transcription. Interestingly, the histone acetyltransferase activity of CBP plays a role in CBP activation of E2-dependent transcription.
Lee
D
D
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, Korea.
Lee
B
B
Kim
J
J
Kim
D W
DW
Choe
J
J
D000123:Acetyltransferases / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D051548:Histone Acetyltransferases; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D015533:Transcriptional Activation
D017362:Cyclic AMP Response Element-Binding Protein; D009856:Oncogene Proteins, Viral; D029701:Saccharomyces cerevisiae Proteins; C069102:oncogene protein E2, Human papillomavirus type 18; D000123:Acetyltransferases; D051548:Histone Acetyltransferases