cDNA cloning, characterization and chromosome mapping of the gene encoding human cartilage associated protein (CRTAP).
We have recently isolated and characterized cDNA clones coding for a novel developmentally regulated avian and mouse embryo protein, CASP for Cartilage Associated Protein. Here we describe the isolation and characterization of the gene coding for the human CASP. The comparison of the putative human and mouse protein sequences with the chick sequence revealed an overall high identity (89% and 51%, respectively). Homology search with known DNA and protein sequences showed that CASPs are related to two mammalian nuclear proteins. Here we demonstrate definitively that CASPs are distinct from these nuclear proteins. However, sequence comparison analyses suggest that all of these proteins belong to a new family. In all human tissues examined two CASP mRNA species were detected, whereas a single mRNA and three mRNAs were found in chick and mouse, respectively. The human CASP gene (CRTAP) was assigned to chromosome 3p22 by fluorescence in situ hybridization.
Tonachini
L
L
Istituto Nazionale per la Ricerca sul Cancro, Centro di Biotecnologie Avanzate, Genova, Italy.
Morello
R
R
Monticone
M
M
Skaug
J
J
Scherer
S W
SW
Cancedda
R
R
Castagnola
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016326:Extracellular Matrix Proteins; D020869:Gene Expression Profiling; D006031:Glycosylation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D021382:Protein Sorting Signals / Q000235:genetics; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D009626:Terminology as Topic
C108053:CRTAP protein, human; C486354:Crtap protein, mouse; D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D018832:Molecular Chaperones; D021382:Protein Sorting Signals; D011506:Proteins; D012333:RNA, Messenger