title: Immunocytochemical techniques to investigate the pathogenesis of infectious micro-organisms and the concurrent immune response of the host.
abstract: For poultry as well as for mammalian species used for scientific research, many immunocytochemical techniques have been developed to investigate in detail the interaction between infectious micro-organisms and the nonspecific and specific immune systems of the host. In this review three techniques have been described with all technical details necessary to perform them correctly: (1) single immunocytochemical staining to detect the infectious micro-organisms in situ at their site of infection, (2) double immunocytochemical staining to visualize simultaneously the infectious micro-organism and the host cellular response to investigate their interactions, and (3) detection of plasma cells producing antibodies specific to the micro-organism. Of the three techniques the results are described when applied on chicken tissues infected with various micro-organisms, such as Marek's disease virus, chicken anemia virus, infectious bursal disease virus and Eimeria tenella.
authors.affiliation: Institute for Animal Science and Health ID-DLO, Department of Immunology, Lelystad, The Netherlands. s.h.m.jeurissen@id.wag-ur.nl
authors.forename: S H
authors.initials: SH
authors.lastname: Jeurissen
authors.forename: E
authors.initials: E
authors.lastname: Claassen
authors.forename: A G
authors.initials: AG
authors.lastname: Boonstra-Blom
authors.forename: L
authors.initials: L
authors.lastname: Vervelde
authors.forename: E
authors.initials: E
authors.lastname: Marga Janse
mesh_terms: D000818:Animals; D002645:Chickens; D003141:Communicable Diseases / Q000382:microbiology / Q000469:parasitology / Q000473:pathology* / Q000662:veterinary* / Q000821:virology; D007107:Immune System / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology / Q000821:virology; D007150:Immunohistochemistry / Q000379:methods*; D011201:Poultry Diseases / Q000382:microbiology / Q000469:parasitology / Q000473:pathology / Q000821:virology