Determination of cyanocobalamin, betamethasone, and diclofenac sodium in pharmaceutical formulations, by high performance liquid chromatography.
The aim of this work was to develop an analytical method for a simultaneous determination of cyanocobalamin (Vitamin B12), betamethasone, and diclofenac, present in pharmaceutical formulations, by high performance liquid chromatography, assuring rapidity, accuracy, precision, and selectivity. The working conditions were as follows: RP18 column of 125 mm x 4 mm ID and a particle size of 5 microm; mobile phase acetonitrile-water (40:60; v/v) (pH* 3.45) adjusted with acetic acidl flow gradient from 0.8 to 1.9 ml/min.; injection volume of 20 microl; temperature 34 degrees C and detection at 240 nm. The method was adequately validated, and linearity, accuracy, as well as the system, method and interday precision, for each active principle, were determined.
González
L
L
Cátedra de Ensayo y Valoración de Medicamentos, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Yuln
G
G
Volonté
M G
MG
D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D001623:Betamethasone / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004008:Diclofenac / Q000032:analysis* / Q000737:chemistry; D005069:Evaluation Studies as Topic; D011358:Product Surveillance, Postmarketing; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D014805:Vitamin B 12 / Q000032:analysis* / Q000737:chemistry
D000097:Acetonitriles; D004008:Diclofenac; D001623:Betamethasone; D014805:Vitamin B 12; D019342:Acetic Acid