Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors.
The presence of laminin-1, collagen-IV, alpha6 and beta1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-1-, beta1-, and alpha6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
Dubový
P
P
Department of Anatomy, Medical Faculty, Brno, Czech Republic.
Svízenská
I
I
Jancálek
R
R
Klusáková
I
I
Houstava
L
L
Haninec
P
P
Zítková
A
A
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D017679:Cryotherapy; D007150:Immunohistochemistry; D039503:Integrin alpha6; D019012:Integrin beta1 / Q000378:metabolism; D016023:Integrins / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*
D015703:Antigens, CD; D039503:Integrin alpha6; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; C121346:laminin 1; D003094:Collagen