Cloning, sequencing, and role in serum susceptibility of porin II from mesophilic Aeromonas hydrophila.
We cloned and sequenced the structural gene for Aeromonas hydrophila porin II from strain AH-3 (serogroup O:34). The genetic position of this gene, like that of ompF in Escherichia coli, is adjacent to aspC and transcribed in the same direction. However, upstream of the porin II gene no similarities with E. coli were found. We obtained defined insertion mutants in porin II gene either in A. hydrophila (O:34) or A. veronii sobria (serogroup O:11) serum-resistant or -sensitive strains. Furthermore, we complemented these mutants with a plasmid harboring only the porin II gene, which allowed us to define the role of porin II as an important surface molecule involved in serum susceptibility and C1q binding in these strains.
Nogueras
M M
MM
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, 08071 Barcelona, Spain.
Merino
S
S
Aguilar
A
A
Benedi
V J
VJ
Tomás
J M
JM
D016980:Aeromonas hydrophila / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins; D001769:Blood / Q000382:microbiology; D015153:Blotting, Western; D003001:Cloning, Molecular; D015922:Complement C1q / Q000378:metabolism; D005816:Genetic Complementation Test; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D018272:Porins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D018272:Porins; C406585:porin II protein, Aeromonas; D015922:Complement C1q