Expression of CD27 on murine hematopoietic stem and progenitor cells.
Hematopoietic stem cells (HSC) are defined by self-renewal and multilineage differentiation potentials. In order to uncover the genetic program of HSC, we utilized high-density arrays to compare gene expression in highly purified mouse HSC and their mature progeny. One molecule specifically expressed in immature cells is CD27, a member of the TNF receptor family previously shown to play roles in lymphoid proliferation, differentiation, and apoptosis. We show here that the CD27 protein is expressed by about 90% of cells in a purified HSC population. Interestingly, the CD27pos cells are enriched for cells with short-term hematopoietic activities (colony forming potential in vivo and in vitro), while the minority CD27neg population is more effective in clonal long-term transplantation.
Wiesmann
A
A
Department of Oncological Sciences, University of Utah, Salt Lake City 84132, USA.
Phillips
R L
RL
Mojica
M
M
Pierce
L J
LJ
Searles
A E
AE
Spangrude
G J
GJ
Lemischka
I
I
D000818:Animals; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000096:biosynthesis*
D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7