[Experimental study on value of carcino-embryonic gama-glutamyl transferase isoenzymes for monitoring carcinogenesis of hepatocytes].
OBJECTIVE
To explore the value of gama-glutamyl transferase (GGT) abnormal expression in monitoring carcinogenesis of hepatocytes.


METHODS
The kinetic alterations of rat (SD) liver pathology, enzymatic histochemistry, total RNA levels, different molecular form GGT and its isoenzymes were investigated on experimental hepatomas inducing with 0.05% 2-fluoenylacetamide (2-FAA).


RESULTS
The GGTs of livers were overexpressed during canceration, liver GGT specific activities (U/g) including soluble and membrane-combined GGT activities of each group in experiment rats were significantly higher (q=4.90, P<0.01) than those of control group. The total RNA concentration was highly positively correlated with liver GGT specific activities or serum GGT activities (r=0.90, r=0.79, P<0.01). The mixed isoenzyme patterns of control rats' and embryo rats' GGT were found in sera and livers of experimental rats, the carcino-embryonic GGT bands can be detected at early stages of rat hepatocyte canceration.


CONCLUSIONS
The present data suggest that GGT is a sensitive enzyme for carcinogenesis of hepatocytes, the analysis of carcino-embryonic GGT is a useful marker for early diagnosis of rat hepatoma.
Yao
D
D
Affiliated Hospital, Nantong Medical College, Nantong 226001, China.
Jiang
D
D
Wu
X
X
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000175:diagnosis / Q000201:enzymology*; D008297:Male; D012313:RNA / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D005723:gamma-Glutamyltransferase / Q000235:genetics / Q000378:metabolism*
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D012313:RNA; D005723:gamma-Glutamyltransferase