title: Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells.
abstract: We have previously shown that the transcription factor AP-1 regulates the expression of genes which allow neoplastically transformed rat fibroblasts to become invasive. Searches for further AP-1 target genes led to the identification of a gene encoding a novel rat kelch family member, named kelch related protein 1 (Krp1). Kelch family members are characterized by a series of repeats at their carboxyl terminus and a BTB/POZ domain near their amino terminus. Rat Krp1 has a primarily cytoplasmic localization, and a small fraction appears to accumulate and co-localize with F-actin at membrane ruffle-like structures in the tips of pseudopodia. Overexpression of Krp1 in transformed rat fibroblasts led to the formation of dramatically elongated pseudopodia, while expression of truncated Krp1 polypeptides resulted in a reduction in the length of pseudopodia. We propose that the transformation-specific expression of Krp1 is required for pseudopod elongation, which are structures that are required for cell motility and invasion.
authors.affiliation: Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD.
authors.forename: H J
authors.initials: HJ
authors.lastname: Spence
authors.forename: I
authors.initials: I
authors.lastname: Johnston
authors.forename: K
authors.initials: K
authors.lastname: Ewart
authors.forename: S J
authors.initials: SJ
authors.lastname: Buchanan
authors.forename: U
authors.initials: U
authors.lastname: Fitzgerald
authors.forename: B W
authors.initials: BW
authors.lastname: Ozanne
mesh_terms: D000199:Actins / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification*; D002471:Cell Transformation, Neoplastic; D003598:Cytoskeletal Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D016761:Oncogene Proteins v-fos / Q000235:genetics; D011554:Pseudopodia / Q000502:physiology*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation; D018631:ras Proteins / Q000235:genetics
chemical_list: D000199:Actins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C586302:KLHL41 protein, human; C405542:Klhl41 protein, rat; D016761:Oncogene Proteins v-fos; D018631:ras Proteins