title: Expression of type XVI collagen in cultured skin fibroblasts is related to cell growth arrest.
abstract: The expression of type XVI collagen in various phases of cell growth in cultured skin fibroblasts was studied. A marked increase in type XVI collagen mRNA level was found in stationary phases of cell growth (non-adherent and confluent phases), whereas the expression of type I and III collagens was undetectable in the non-adherent phase but became greater in the confluent phase. When suspended cells were further cultured over 72 h (suspension arrest), mRNA level and gene transcription of type XVI collagen were time-dependently increased whereas those of type I collagen remained undetectable. When the confluent cells were further cultured for 72 h under the condition of serum deprivation (serum deprivation arrest), mRNA levels of both type XVI collagen and type I collagen were elevated. The level of type XVI collagen polypeptide in the culture media of suspension-arrested and serum deprivation-arrested cells paralleled the mRNA level of type XVI collagen. The results indicate that expression of type XVI collagen (a member of the fibril-associated collagens with interrupted triple helices), unlike interstitial collagens (type I collagen), is related to cell growth arrest brought about by two different growth inhibiting systems, suspension arrest and serum deprivation arrest.
authors.affiliation: Department of Dermatology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, Japan. tajimas@ndmc.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Tajima
authors.forename: A
authors.initials: A
authors.lastname: Akagi
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Ishibashi
authors.forename: A
authors.initials: A
authors.lastname: Kawada
authors.forename: N
authors.initials: N
authors.lastname: Yamaguchi
mesh_terms: D002448:Cell Adhesion; D002455:Cell Division / Q000235:genetics*; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D016895:Culture Media, Serum-Free; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
chemical_list: D016895:Culture Media, Serum-Free; D012333:RNA, Messenger; D003094:Collagen