IL-1beta and ICE mRNA are not altered upon beta-amyloid(25-35) induced neurotoxicity in human neuroblastoma cells.
The specific beta-amyloid(25-35) fragment induced cellular degradation of the human neuroblastoma cell line SH-SY5Y, but did not elicit an effect on the levels of interleukin-1beta and interleukin-1beta converting enzyme, as determined by semiquantitative reverse transcription-polymerase chain reaction and immunocytochemical analysis. The assays revealed constitutive expression of these proteins both at mRNA and protein level. It is conceivable that in the absence of glial elements, such as in the present neuroblastoma cell line, beta-amyloid triggers the toxicity through a direct action and/or through the production of other harmful molecules.
Diana
A
A
Department of Clinical Neuroscience, Occupational Therapy and Elderly Care Research, Division of Geriatric Medicine, Karolinska Institute, Novum, KFC, S-141 86, Huddinge, Sweden. diana@unica.it
Eriksson
C
C
Winblad
B
B
Schultzberg
M
M
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D016229:Amyloid beta-Peptides / Q000633:toxicity*; D020170:Caspase 1 / Q000032:analysis / Q000235:genetics*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D020287:In Situ Nick-End Labeling; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics*; D009410:Nerve Degeneration / Q000139:chemically induced / Q000201:enzymology; D009447:Neuroblastoma; D009474:Neurons / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D010446:Peptide Fragments / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology
D000590:Amino Acid Chloromethyl Ketones; D016229:Amyloid beta-Peptides; D015853:Cysteine Proteinase Inhibitors; D007375:Interleukin-1; C098738:N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone; D010446:Peptide Fragments; D012333:RNA, Messenger; C074636:amyloid beta-protein (25-35); D020170:Caspase 1