title: Growth factor-like effects of placental alkaline phosphatase in human fetus and mouse embryo fibroblasts.
abstract: Human placental alkaline phosphatase (PALP) is synthesized in the placenta during pregnancy and is also expressed in many cancer patients; however, its physiological role is unknown. Here we show that in human fetus fibroblasts as well as normal and H-ras-transformed mouse embryo fibroblasts PALP stimulates DNA synthesis and cell proliferation in synergism with insulin, zinc and calcium. The mitogenic effects of PALP are associated with the activation of c-Raf-1, p42/p44 mitogen-activated protein kinases, p70 S6 kinase, Akt/PKB kinase and phosphatidylinositol 3'-kinase. The results suggest that in vivo PALP may promote fetus development as well as the growth of cancer cells which express oncogenic Ras.
authors.affiliation: The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA.
authors.forename: Q B
authors.initials: QB
authors.lastname: She
authors.forename: J J
authors.initials: JJ
authors.lastname: Mukherjee
authors.forename: J S
authors.initials: JS
authors.lastname: Huang
authors.forename: K S
authors.initials: KS
authors.lastname: Crilly
authors.forename: Z
authors.initials: Z
authors.lastname: Kiss
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002455:Cell Division; D002461:Cell Line, Transformed; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D005347:Fibroblasts / Q000378:metabolism*; D058851:GPI-Linked Proteins; D011905:Genes, ras; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010920:Placenta / Q000201:enzymology* / Q000378:metabolism
chemical_list: D058851:GPI-Linked Proteins; D006133:Growth Substances; D007328:Insulin; D007527:Isoenzymes; D020928:Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental; D002118:Calcium