Granulocyte-colony stimulating factor (G-CSF)-primed, delayed marrow harvests as a source of hematopoietic stem and progenitor cells for allogeneic transplantation.
We evaluated the ability of G-CSF to increase the number of hematopoietic stem cells obtained by "delayed" BM harvest for allogeneic transplantation. Five normal donors received G-CSF @ 10 mcg/kg/day x 5 followed by repeat PB and BM assays at day 6 and 16, and BM harvest at day 16. Stem cells were not increased in the BM at day 16. Five patients underwent BMT and engrafted at +10 to +19 days. While the tested strategy offers no intrinsic advantages, its potential cannot be evaluated fully without alternative timing and/or additional, "early acting" growth factors.
Phillips
G L
GL
Department of Medicine and Markey Cancer Center, University of Kentucky Chandler Medical Center, Chandler Lexington, USA. gordon@pop.uky.edu
Davey
D D
DD
Hale
G A
GA
Marshall
K W
KW
Munn
R K
RK
Nath
R
R
Reece
D E
DE
Van Zant
G
G
D001782:Blood Donors; D002452:Cell Count; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D013997:Time Factors; D014184:Transplantation, Homologous
D016179:Granulocyte Colony-Stimulating Factor