Ex vivo T-lymphocyte derived cytokine production in SIRS patients is influenced by experimental procedures.
Ex vivo production of interleukin-2 (IL-2), IL-4, IL-5, and IL-10 by peripheral blood mononuclear cells (PBMC) was studied in 13 septic patients with infectious systemic inflammatory response syndrome (SIRS) and 13 patients undergoing cardiac surgery with cardiopulmonary bypass (CPB) (noninfectious SIRS). We have investigated the levels of cytokines after activation by either concanavalin A (ConA), phytohemagglutinin (PHA), or anti-CD3 antibodies. In whole blood assays, ConA-induced IL-10 was significantly reduced in both groups of patients compared with healthy controls. In sepsis patients, IL-2, IL-5, and IL-10 productions by isolated PBMC were diminished on ConA-induced activation but not in response to PHA and anti-CD3; in CPB patients, only anti-CD3-induced IL-10 production was significantly reduced. Our data indicate that subtle modifications of the reactivity of circulating cells occur during infectious and noninfectious SIRS. Production of both Th1 and Th2 cytokines can be down-regulated; however, the nature of the SIRS, of the cell population, and of the activator may influence the observation.
Muret
J
J
Department of Anaesthesia and Intensive Care Medicine, Hôpital Lariboisière, Paris, France.
Marie
C
C
Fitting
C
C
Payen
D
D
Cavaillon
J M
JM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000758:Anesthesia / Q000009:adverse effects; D000906:Antibodies / Q000494:pharmacology; D017252:CD3 Complex; D016022:Case-Control Studies; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D007378:Interleukins / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D018746:Systemic Inflammatory Response Syndrome / Q000378:metabolism* / Q000382:microbiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
D000906:Antibodies; D017252:CD3 Complex; D016207:Cytokines; D007378:Interleukins; D010835:Phytohemagglutinins; D003208:Concanavalin A; D016753:Interleukin-10