title: A novel member of the BTB/POZ family, PATZ, associates with the RNF4 RING finger protein and acts as a transcriptional repressor.
abstract: We have identified a novel human gene encoding a 59-kDa POZ-AT hook-zinc finger protein (PATZ) that interacts with RNF4, a mediator of androgen receptor activity, and acts as a transcriptional repressor. PATZ cDNA was isolated through a two-hybrid interaction screening using the RING finger protein RNF4 as a bait. In vitro and in vivo interaction between RNF4 and PATZ was demonstrated by protein-protein affinity chromatography and coimmunoprecipitation experiments. Such interaction occurred through a small region of PATZ containing an AT-hook DNA binding domain. Immunofluorescence staining and confocal microscopy showed that PATZ localizes in distinct punctate nuclear regions and colocalizes with RNF4. Functional analysis was performed by cotransfection assays: PATZ acted as a transcriptional repressor, whereas its partner RNF4 behaved as a transcriptional activator. When both proteins were overexpressed a strong repression of the basal transcription was observed, indicating that the association of PATZ with RNF4 switches activation to repression. In addition, RNF4 was also found to associate with HMGI(Y), a chromatin-modeling factor containing AT-hook domains.
authors.affiliation: Centro di Endocrinologia ed Oncologia Sperimentale del CNR "G. Salvatore" Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano" Università degli Studi di Napoli "Federico II" via S. Pansini, 5, 80131 Napoli, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Fedele
authors.forename: G
authors.initials: G
authors.lastname: Benvenuto
authors.forename: R
authors.initials: R
authors.lastname: Pero
authors.forename: B
authors.initials: B
authors.lastname: Majello
authors.forename: S
authors.initials: S
authors.lastname: Battista
authors.forename: F
authors.initials: F
authors.lastname: Lembo
authors.forename: E
authors.initials: E
authors.lastname: Vollono
authors.forename: P M
authors.initials: PM
authors.lastname: Day
authors.forename: M
authors.initials: M
authors.lastname: Santoro
authors.forename: L
authors.initials: L
authors.lastname: Lania
authors.forename: C B
authors.initials: CB
authors.lastname: Bruni
authors.forename: A
authors.initials: A
authors.lastname: Fusco
authors.forename: L
authors.initials: L
authors.lastname: Chiariotti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000378:metabolism; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011485:Protein Binding; D012097:Repressor Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers
chemical_list: C402243:ABTB1 protein, human; C509774:Abtb1 protein, mouse; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; C412784:PATZ1 protein, human; C114070:RNF4 protein, human; D012097:Repressor Proteins; D014157:Transcription Factors; C485875:Zfp278 protein, mouse; D025741:HMGA1a Protein; C486410:Rnf4 protein, mouse; D044767:Ubiquitin-Protein Ligases