title: WECHE: a novel hematopoietic regulatory factor.
abstract: Previously, we described AGM-derived endothelial cell lines that either inhibited or permitted the development of erythroid or B cells. We utilized a differential gene expression method to isolate a chemokine, termed WECHE, from one of these cell lines. WECHE inhibited the formation of erythroid cells but had no effect on either myeloid or B cell formation. WECHE repressed BFU-E development from either mouse fetal liver or bone marrow progenitor cells but had no effect on colony formation induced by IL-3 or IL-7. WECHE reduced HPP-CFC production from fetal liver-derived stem cells. WECHE hindered the growth of yolk sac-derived endothelial cells. WECHE was also chemotactic for bone marrow cells. Thus, WECHE is a novel chemokine that regulates hematopoietic differentiation.
authors.affiliation: Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan. oohneda@kaiju.medic.kumamoto-u.ac.jp
authors.forename: O
authors.initials: O
authors.lastname: Ohneda
authors.forename: K
authors.initials: K
authors.lastname: Ohneda
authors.forename: H
authors.initials: H
authors.lastname: Nomiyama
authors.forename: Z
authors.initials: Z
authors.lastname: Zheng
authors.forename: S A
authors.initials: SA
authors.lastname: Gold
authors.forename: F
authors.initials: F
authors.lastname: Arai
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: B E
authors.initials: BE
authors.lastname: Taillon
authors.forename: R A
authors.initials: RA
authors.lastname: McIndoe
authors.forename: R A
authors.initials: RA
authors.lastname: Shimkets
authors.forename: D A
authors.initials: DA
authors.lastname: Lewin
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: L A
authors.initials: LA
authors.lastname: Lasky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D018925:Chemokines / Q000235:genetics / Q000502:physiology*; D019743:Chemokines, CXC; D002633:Chemotaxis / Q000502:physiology; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D018925:Chemokines; D019743:Chemokines, CXC; C118893:Cxcl15 protein, mouse