Nonaqueous versus aqueous capillary electrophoresis for the dosage of N-butylscopolamine in various pharmaceutical formulations.
A simple nonaqueous capillary electrophoresis method is described for the separation of several atropine and scopolamine related drugs. The analysis of these pharmaceutical compounds was performed in a methanol-acetonitrile (25/5, v/v) mixture containing 25 mM ammonium acetate and 1 M acetic acid. The robustness was proved using a full factorial design at two levels. The method was validated and successfully applied for the determination of N-butylscopolamine in different pharmaceutical preparations. Results were compared to those obtained by a capillary electrophoresis method based on aqueous media.
Cherkaoui
S
S
Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Pavillon des Isotopes, Switzerland.
Mateus
L
L
Christen
P
P
Veuthey
J L
JL
D002086:Butylscopolammonium Bromide / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D019075:Electrophoresis, Capillary / Q000379:methods*; D018727:Muscarinic Antagonists / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results
D018727:Muscarinic Antagonists; D002086:Butylscopolammonium Bromide