Voltammetric determination of lactate dehydrogenase using a carbon paste electrode.
A voltammetric study was performed by linear sweep voltammetry using a carbon paste electrode, in -0.1 to +1.3 V potential range, with the view of elaborating an assay for lactate dehydrogenase (LDH) in different enzymatic preparations and biological fluids. There have been performed studies concerning pH influence upon the enzymatic reaction, as well as the electrochemical behavior of LDH in the presence of modified carbon paste electrodes saturated with sodium pyruvate and/or NADH.
Tarmure
C
C
Biochemistry and Clinical Laboratory Department, University of Medicine and Pharmacy Iuliu Haţieganu, Faculty of Pharmacy, Cluj-Napoca, Romania.
Săndulescu
R
R
Ionescu
C
C
D002138:Calibration; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*
D007770:L-Lactate Dehydrogenase