Flow injection analysis of mercury(II) in pharmaceuticals based on enzyme inhibition and biosensor detection.
An enzymatic amperometric procedure for measurement of mercury(II) in pharmaceuticals, based on the inhibition of invertase and on a glucose electrode was studied. Analytical parameters for measurements in batch and flow injection analysis (FIA) have been optimised. Mercury(II) was detected in the 10-60 ppb range with RSD < or =2%. A sample throughput of 6 h(-1) for batch and 15 h(-1) for FIA was obtained. The total mercury(II) from thimerosal (thiomersal, sodium ethylmercurithiosalicylate) in eye-drop samples was measured with the amperometric procedure after oxidative cleavage treatment. Results for both batch and FIA procedures correlated well with atomic absorbtion spectroscopy (AAS) data.
Bertocchi
P
P
Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanita, Rome, Italy.
Ciranni
E
E
Compagnone
D
D
Magearu
V
V
Palleschi
G
G
Pirvutoiu
S
S
Valvo
L
L
D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004800:Enzymes, Immobilized; D017022:Flow Injection Analysis; D005947:Glucose / Q000032:analysis; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors*; D007202:Indicators and Reagents; D008628:Mercury / Q000032:analysis* / Q000494:pharmacology; D013054:Spectrophotometry, Atomic; D013395:Sucrose / Q000032:analysis; D043324:beta-Fructofuranosidase
D004791:Enzyme Inhibitors; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D013395:Sucrose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D008628:Mercury; D005947:Glucose