Cardiac membrane proteins and phospholipids in L-NAME induced hypertension.
This study deals with qualitative alterations of membranes in cardiac cells after chronic inhibition of NO synthesis in rats induced by administration of N(G)-nitro-L-arginine methyl ester (L-NAME) in a dose 40 mg/kg/day for 4 weeks. Concentration of proteins did not change either in the cytosolic fraction or in the particulate fraction of the cardiac homogenate from L-NAME treated rats. The concentration of phospholipids and consequently the ratio of phospholipids to membrane proteins increased by 100% and 88%, respectively. The concentration of conjugated dienes (CD), often used as an indirect marker for the production of free oxygen radicals, increased by 141% after calculation per gram of tissue. However, evaluation of CD concentration directly in phospholipids revealed no change, suggesting that phospholipids in cardiac tissue after L-NAME treatment were not damaged additionally, by increased level of free oxygen radicals. The increase of the CD concentration in the cardiac tissue is therefore, a consequence of the elevated phospholipid concentration.
Ziaciková
J
J
Institute for Heart Research, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Pechánová
O
O
Bernátová
I
I
Vrbjar
N
N
D000818:Animals; D004791:Enzyme Inhibitors / Q000633:toxicity; D006973:Hypertension / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000633:toxicity*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D017382:Reactive Oxygen Species / Q000378:metabolism
D004791:Enzyme Inhibitors; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D017382:Reactive Oxygen Species; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester