Experimental African trypanosomiasis: differences in cytokine and nitric oxide production by macrophages from resistant and susceptible mice.
Immunosuppression in experimental infections with Trypanosoma congolense is mediated by the synergistic action of macrophages and a novel lymphocyte(s), which involves the activity of IFN-gamma as well as IL-10. BALB/c mice are highly susceptible while C57Bl/6 mice are relatively resistant to T. congolense infections. Plasma and/or supernatants of spleen cell cultures of infected susceptible BALB/c mice have more IL-10 but less IL-12 than those of infected relatively resistant C57Bl/6 mice. Cells of a BALB/c macrophage cell line, when pulsed with T. congolense, produce more IL-10 and IL-6, but have less TNF-alpha mRNA, than equally treated cells of a C57Bl/6 cell line. Peritoneal and/or bone marrow-derived macrophages obtained from BALB/c mice, pulsed with T. congolense in culture, produce less nitric oxide, TNF-alpha and IL-12, but more IL-6 and IL-10 than equally treated macrophages isolated from C57Bl/6 mice. We suggest that genetic resistance to African trypanosomiasis is expressed at the level of the macrophage.
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Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
Kaushik
R S
RS
Uzonna
J
J
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology / Q000469:parasitology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D004195:Disease Models, Animal; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D007108:Immune Tolerance; D007113:Immunity, Innate / Q000276:immunology; D008213:Lymphocyte Activation; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D010587:Phagocytosis; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000469:parasitology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014348:Trypanosoma congolense / Q000276:immunology; D014353:Trypanosomiasis, African / Q000235:genetics / Q000276:immunology*
D016207:Cytokines; D009569:Nitric Oxide