title: Development of a hypersensitive detection method for human parvovirus B19 DNA.
abstract: A new detection method for human parvovirus B19 DNA was established using PCR coupled with a hybridization protection assay. The amplified product was detected using acridinium ester-labeled DNA probes. By this method, a few copies of B19 DNA were detected in human serum albumin.
authors.affiliation: Chugai Pharmaceutical Co. Ltd., Central Research Laboratories, 3-41-8 Takada, Toshima-ku, Tokyo 171-8545, Japan. satokza@chugai-pharm.co.jp
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: E
authors.initials: E
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Kamisango
authors.forename: H
authors.initials: H
authors.lastname: Iwasaki
authors.forename: S
authors.initials: S
authors.lastname: Matsubara
authors.forename: Y
authors.initials: Y
authors.lastname: Matsunaga
mesh_terms: D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D010322:Parvoviridae Infections / Q000097:blood / Q000175:diagnosis*; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D012709:Serum Albumin; D019562:Viral Load
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D012709:Serum Albumin