title: Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin.
abstract: The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g-1 fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g-1 fr. wt., while the growth of the calli was enhanced (25% increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding 13C2-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C2-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally--and biogenetically--related naphthylisoquinoline alkaloids.
authors.affiliation: Institut für Organische Chemie der Universität, Universität Würzburg, Germany. bringman@chemie.uni-wuerzburg.de
authors.forename: G
authors.initials: G
authors.lastname: Bringmann
authors.forename: H
authors.initials: H
authors.lastname: Rischer
authors.forename: M
authors.initials: M
authors.lastname: Wohlfarth
authors.forename: J
authors.initials: J
authors.lastname: Schlauer
authors.forename: L A
authors.initials: LA
authors.lastname: Assi
mesh_terms: D002478:Cells, Cultured; D009285:Naphthoquinones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D059828:Plant Cells; D010944:Plants / Q000378:metabolism*
chemical_list: D009285:Naphthoquinones