title: Visualization of the in vivo generation of donor antigen-specific effector CD8+ T cells during mouse cardiac allograft rejection: in vivo effector CD8+ T cell generation during allograft rejection.
abstract: BACKGROUND
  An adoptive transfer system was used to study the fate of alloreactive CD8+ H-2Kb-specific TCR transgenic (DES+) T cells in vivo after transplantation.
  
  
  METHODS
  A trace population of 2.0x10(6) CD8+DES+ T cells were adoptively transferred into syngeneic CBA.Ca (H-2k) mice 24 hr before transplantation of an H-2Kb+ or H-2Kb- cardiac allograft.
  
  
  RESULTS
  H-2Kb specific T cells proliferated and produced interleukin-2 and interferon-gamma in response to H-2Kb+, but not H-2Kb- cardiac allografts. CD8+DES+ T cells that infiltrated the H-2Kb+ cardiac allografts developed a distinct cell surface and cytokine phenotype compared with the CD8+DES+ T cells that remained in the periphery. H-2Kb-specific graft infiltrating T cells (a) underwent a larger number of cell divisions (> =3), (b) increased in size, (c) up-regulated CD69, and (d) down-regulated CD62L.
  
  
  CONCLUSIONS
  These results demonstrate that alloantigen-specific T cells can be monitored in vivo during the immune response to an allograft and that the fate of CD8+ T cells specific for the allogeneic class I molecules expressed by the graft is different between cells in the periphery and those that infiltrate the graft.
authors.affiliation: Nuffield Department of Surgery, University of Oxford, Headington, United Kingdom.
authors.forename: B J
authors.initials: BJ
authors.lastname: Gilot
authors.forename: M
authors.initials: M
authors.lastname: Hara
authors.forename: N D
authors.initials: ND
authors.lastname: Jones
authors.forename: A
authors.initials: A
authors.lastname: van Maurik
authors.forename: M
authors.initials: M
authors.lastname: Niimi
authors.forename: V
authors.initials: V
authors.lastname: Hadjianastassiou
authors.forename: P J
authors.initials: PJ
authors.lastname: Morris
authors.forename: K J
authors.initials: KJ
authors.lastname: Wood
mesh_terms: D019264:Adoptive Transfer / Q000379:methods; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology; D002455:Cell Division / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D000939:Epitopes; D005434:Flow Cytometry; D006084:Graft Rejection / Q000378:metabolism / Q000473:pathology; D006085:Graft Survival / Q000276:immunology; D016027:Heart Transplantation / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000187:drug effects; D015759:Ionomycin / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014019:Tissue Donors; D014184:Transplantation, Homologous / Q000473:pathology
chemical_list: D016207:Cytokines; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D015759:Ionomycin; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate