title: Stapled versus hand-sewn vascular anastomoses. An experimental study in the rabbit.
abstract: OBJECTIVE
  Narrowing of vascular anastomoses is a frequently encountered surgical problem, with intimal hyperplasia being one of its most important causes. The aim of the present study was to compare in a rabbit model 'manual' (hand-sewn) with 'stapled' anastomoses (using a staple device) with respect to occurrence and severity of intimal hyperplasia.
  
  
  METHODS
  Twenty-four male rabbits (mean weight 2,849 g) were randomly allocated to one of two groups (n = 12). An end-to-end anastomosis of the left femoral artery was performed in all animals under general anesthesia. The anastomosis was hand sewn in group 1, while a vascular closure stapler (VCS) was used in group 2. Both anastomotic time and total operation time were recorded. After 28 days, the rabbits were sacrificed. The femoral artery of operated and nonoperated sides were removed and prepared for anatomopathological examination. The I/M ratio (= difference between tunica intima and tunica media) was determined on hematoxylin-eosin stained slides. All results were analyzed using Student's t test.
  
  
  RESULTS
  Mean anastomotic times were 25 +/- 7 min for the 'manual' group and 17 +/- 9 min for the 'stapled' group (p = 0.02). There was no significant difference in the total operation time (55 +/- 15 vs. 41 +/- 18 min, p = 0.057). All animals survived the anastomosis procedure. In the group of 'manual' anastomosis, morbidity was significantly higher. At the moment of sacrifice, all anastomoses were patent. There was no difference in the I/M ratio between the groups.
  
  
  CONCLUSIONS
  The use of VCS is a promising alternative to hand-sewn anastomoses. It takes less time to perform a stapled anastomosis, the technique has a shorter learning period and morbidity seems to be lower when vascular anastomoses are applied with the VCS in this rabbit model.
authors.affiliation: Laboratory for Surgical Research, University of Antwerp, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Pauli
authors.forename: P
authors.initials: P
authors.lastname: Lauwers
authors.forename: R
authors.initials: R
authors.lastname: Van Hee
authors.forename: R
authors.initials: R
authors.lastname: Discart
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D005263:Femoral Artery / Q000601:surgery*; D008297:Male; D011817:Rabbits; D013536:Suture Techniques; D013537:Sutures