Analysis of biogenic amines by solid-phase microextraction and high-performance liquid chromatography with electrochemical detection.
We investigated the new solid-phase microextraction method by high-performance liquid chromatography with electrochemical detection for the analysis of biogenic amines. The Carbowax-Templated Resin 50 microm (purple) fibre coating offers good performances for dopamine and serotonin separation, i.e., good selectivity and high sensibility (0.1 microg l(-1)). We also tested this fibre for biogenic amines quantification of rat striatum. The coating seems to be selective towards the amines and has low affinity for the metabolites, allowing a good separation and preventing drawbacks from the biological matrix. These first results obtained using this original separation method offer large perspectives of application to many biological samples.
Auger
J
J
Institut de Recherche sur la Biologie de l'Insecte, Université François Rabelais, Tours, France. auger@univ-tours.fr
Boulay
R
R
Jaillais
B
B
Delion-Vancassel
S
S
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D001679:Biogenic Amines