Solid-phase synthesis and biological screening of N-alpha-mercaptoamide template-based matrix metalloprotease inhibitors.
A series of N-alpha-mercaptoacetyl containing dipeptides have been prepared on solid-phase supports as putative matrix metalloprotease (MMP) inhibitors. Inhibitor design was based on a positional scanning approach of the amino acids present within a template molecule, previously shown to be an MMP inhibitor with good pharmacological characteristics. This study is the first step in a unique programme, designed to expand the repertoire of molecular templates which can be chosen as starting points for the development of more focused parallel and/or combinatorial libraries of MMP inhibitors as a means to accelerate the lead discovery process. This paper reports the success of such an approach in the development of agents with activity against a number of pathologically important MMPs. After screening of these positional scanning libraries, we have obtained important SAR information, in particular, pharmacophores with the ability to impart selectivity for particular MMP species.
Lynas
J F
JF
Division of Biomedicinal Chemistry, School of Pharmacy, Queen s University Belfast, Medical Biology Centre, 79 Lisburn Road, Belfast, BT9 7BL, Northern Ireland, UK. j.lynas@qub.ac.uk
Martin
S L
SL
Walker
B
B
Baxter
A D
AD
Bird
J
J
Bhogal
R
R
Montana
J G
JG
Owen
D A
DA
D005470:Fluorometry; D061965:Matrix Metalloproteinase Inhibitors; D011480:Protease Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D061965:Matrix Metalloproteinase Inhibitors; C406102:N-mercaptoamide; D011480:Protease Inhibitors; D013438:Sulfhydryl Compounds