title: Microsecond to minute dynamics revealed by EX1-type hydrogen exchange at nearly every backbone hydrogen bond in a native protein.
abstract: A previous comprehensive analysis of the pH dependence of native-state amide hydrogen (NH) exchange in turkey ovomucoid third domain (OMTKY3) yielded apparent opening and closing rate constants (k(op) and k(cl)) at 14 NH groups involved in global conformational changes. This analysis has been extended to 18 additional slowly exchanging NH groups. Quench-flow experiments were performed to monitor NH exchange in native OMTKY3 from neutral to very alkaline pH ( approximately 12) conditions. Above pH 10 the mechanism of exchange switched from one governed by a rapid equilibrium preceding the chemistry of exchange (i.e. EX2 exchange), to one where exchange was limited by the rate of opening (i.e. EX1 exchange). Kinetics of solvent exposure are now known for nearly all backbone NH groups in native OMTKY3, yielding rate constants that span five orders of magnitude, 0.004 to 200 s(-1).
authors.affiliation: Department of Biochemistry, the University of Iowa, Iowa City 52242, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Arrington
authors.forename: A D
authors.initials: AD
authors.lastname: Robertson
mesh_terms: D000577:Amides / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D006859:Hydrogen / Q000378:metabolism*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009038:Motion; D019906:Nuclear Magnetic Resonance, Biomolecular; D010059:Ovomucin / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012997:Solvents; D013816:Thermodynamics; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000378:metabolism; D014422:Turkeys; D014867:Water / Q000378:metabolism
chemical_list: D000577:Amides; D012997:Solvents; D014361:Trypsin Inhibitors; D014867:Water; D010059:Ovomucin; D006859:Hydrogen