Determination of intracranial tumor volumes in a rodent brain using magnetic resonance imaging, Evans blue, and histology: a comparative study.
The measurement of tumor volumes is a practical and objective method of assessing the efficacy of a therapeutic agent. However, the relative accuracy of different methods of assessing tumor volume has been unclear. Using T1-weighted, gadolinium-enhanced magnetic resonance Imaging (T1-MRI), Evans Blue infusion and histology we measured intracranial tumor volumes in a rodent brain tumor model (RT2) at days 10, 16 and 18 after implantation of cells in the caudate putamen. There is a good correlation between tumor volumes comparing T1-MRI and Evans Blue (r2 = 0.99), T1-MRI and Histology (r2 = 0.98) and histology and Evans Blue (r2 = 0.93). Each of these methods is reliable in estimating tumor volumes in laboratory animals. There was significant uptake of gadolinium and Evans Blue in the tumor suggesting a wide disruption of the blood-brain barrier.
Prabhu
S S
SS
Division of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA.
Broaddus
W C
WC
Oveissi
C
C
Berr
S S
SS
Gillies
G T
GT
D000818:Animals; D001812:Blood-Brain Barrier; D001932:Brain Neoplasms / Q000473:pathology*; D002138:Calibration; D005070:Evans Blue / Q000032:analysis; D005260:Female; D019786:Gadolinium DTPA; D006652:Histological Techniques; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D005070:Evans Blue; D019786:Gadolinium DTPA