title: Two reproducible and sensitive liquid chromatographic methods to quantify atenolol and propranolol in human plasma and determination of their associated analytical error functions.
abstract: Two liquid chromatography (LC) methods with fluorimetric detection have been developed to measure atenolol and propranolol in human plasma. The same 5 microm Nucleosil RP-18 column, extraction procedure and mobile phase (containing acetonitrile, water, triethylamine and phosphoric acid, pH 3) were used. The linearity ranges were 25-800 ng/ml for atenolol and 3.13-100 ng/ml for propranolol. The coefficients of variation for validation assays were lower than 15% at the concentration assayed. The functions of the analytical error were linear: SD (ng/ml)=7.698+0.037C for atenolol and SD (ng/ml)=0.126+0.036C for propranolol.
authors.affiliation: Department of Pharmacy and Pharmaceiutical Technology, Faculty of Pharmacy, University of Barcelona, Spain.
authors.forename: A J
authors.initials: AJ
authors.lastname: Braza
authors.forename: P
authors.initials: P
authors.lastname: Modamio
authors.forename: E L
authors.initials: EL
authors.lastname: Mariño
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood*; D001262:Atenolol / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D011433:Propranolol / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D000319:Adrenergic beta-Antagonists; D001262:Atenolol; D011433:Propranolol