Plasma cell generation from B-lymphocytes via CD27/CD70 interaction.
To produce antibodies, the differentiation of B cells into antibody-secreting cells, plasma cells, is required. We describe that ligation of CD27, which belongs to the tumor necrosis factor receptor (TNFR) family and is a memory marker of B cells, yields crucial signals that positively control the entry of B cells into the pathway to plasma cells. The triggering via CD27 by CD27 ligand (CD70) on purified peripheral blood B cells yielded an increase in the number of plasma cells in the presence of interleukin-10 (IL-10). The differentiation into plasma cells by a combination of IL-10 and CD70-transfectants occurred in CD27+ B cells, but not in CD27- B cells. Moreover, the addition of IL-2 to the IL-10 and CD70-transfectants greatly induced the differentiation into plasma cells. In the presence of only IL-2, IL-4 or IL-6, CD70-transfectants did not promote the differentiation into plasma cells. On the other hand, CD40 signaling increased the expansion of a B cell pool from peripheral blood B cells primarily activated by IL-2, IL-10 and anti-CD40 mAb. These data demonstrate that CD27 ligand (CD70) is a key molecule to direct the differentiation of CD27+ memory B cells toward plasma cells in cooperation with IL-10.
Agematsu
K
K
From the Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan. agemats@gipac.shinshu-u.ac.jp
Hokibara
S
S
Nagumo
H
H
Shinozaki
K
K
Yamada
S
S
Komiyama
A
A
D000818:Animals; D015703:Antigens, CD; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D053282:CD27 Ligand; D002454:Cell Differentiation / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D010950:Plasma Cells / Q000276:immunology* / Q000473:pathology; D015398:Signal Transduction / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology*
D015703:Antigens, CD; D053282:CD27 Ligand; C505771:CD70 protein, human; D008565:Membrane Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7