Mitochondrial membrane potential and neuronal glutamate excitotoxicity: mortality and millivolts.
In the past few years it has become apparent that mitochondria have an essential role in the life and death of neuronal and non-neuronal cells. The central mitochondrial bioenergetic parameter is the protonmotive force, Deltap. Much research has focused on the monitoring of the major component of Deltap, the mitochondrial membrane potential Deltapsim, in intact neurones exposed to excitotoxic stimuli, in the hope of establishing the causal relationships between cell death and mitochondrial dysfunction. Several fluorescent techniques have been used, and this article discusses their merits and pitfalls.
Nicholls
D G
DG
Neurosciences Institute, Dept of Pharmacology and Neuroscience, University of Dundee, Dundee, UK DD1 9SY.
Ward
M W
MW
D000818:Animals; D004594:Electrophysiology; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D008566:Membranes; D008928:Mitochondria / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D017470:Receptors, Glutamate / Q000187:drug effects*
D018690:Excitatory Amino Acid Agonists; D017470:Receptors, Glutamate