Flow-injection fluorimetric determination of 1,4-benzodiazepines in pharmaceutical formulations after acid hydrolysis.
A simple, rapid and fully automated flow injection method with fluorimetric detection after hydrolysis with H2SO4 in ethanolic or methanolic medium at room temperature has been developed for the determination of 1,4-benzodiazepines (oxazepam, diazepam and nitrazepam) in pharmaceutical formulations. The calibration curves are linear in the ranges (mg ml(-1)) of oxazepam (0.025-0.150), diazepam (0.010-0.125) and nitrazepam (0.010-0.150), with detection limits of 0.01, 0.005 and 0.005 mg ml(-1), respectively, and RSD (1% (n = 10). The measurement throughput is 60 h(-1) using a 200-microl sample volume obtained by the direct dissolution of formulations in alcohol.
Dolejsová
J
J
Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic.
Solich
P
P
Polydorou
C K
CK
Koupparis
M A
MA
Efstathiou
C E
CE
D014151:Anti-Anxiety Agents / Q000032:analysis; D001322:Autoanalysis; D001569:Benzodiazepines / Q000032:analysis*; D002138:Calibration; D003975:Diazepam / Q000032:analysis; D017022:Flow Injection Analysis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D009567:Nitrazepam / Q000032:analysis; D010076:Oxazepam / Q000032:analysis; D012044:Regression Analysis; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D014151:Anti-Anxiety Agents; D007202:Indicators and Reagents; D001569:Benzodiazepines; D010076:Oxazepam; D009567:Nitrazepam; D003975:Diazepam