Mechanistic studies on type I and type II dehydroquinase with (6R)- and (6S)-6-fluoro-3-dehydroquinic acids.
(6R)- and (6S)-6-Fluoro-3-dehydroquinic acids are shown to be substrates for type I and type II dehydroquinases. Their differential reactivity provides insight into details of the reaction mechanism and enables a novel enzyme-substrate imine to be trapped on the type I enzyme.
Parker
E J
EJ
University Chemical Laboratory, Cambridge, UK.
González Bello
C
C
Coggins
J R
JR
Hawkins
A R
AR
Abell
C
C
D003509:Cyclohexanecarboxylic Acids / Q000378:metabolism*; D003512:Cyclohexanones / Q000378:metabolism*; D006836:Hydro-Lyases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D013237:Stereoisomerism
D003509:Cyclohexanecarboxylic Acids; D003512:Cyclohexanones; D007527:Isoenzymes; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase