Role of chicken IL-2 on gammadelta T-cells and Eimeria acervulina-induced changes in intestinal IL-2 mRNA expression and gammadelta T-cells.
Continuous culture of concanavalin A (Con A)-activated spleen cells in the presence of chicken recombinant IL-2 (rIL-2) promoted preferential growth of gammadelta T-cells. These cells displayed a high level of spontaneous cytotoxicity against LSCC-RP9 tumor cells, an avian NK cell target. Stimulation of IL-2-dependent gammadelta T-cells with Con A induced IFN-gamma and IL-2 mRNA transcripts, whereas stimulation with rIL-2 induced only IFN-gamma mRNA. Subcutaneous injection of 3-week-old chickens with IL-2 DNA increased splenic cells, expressing the CD8 and gammadelta TCR antigens. To investigate the role of IL-2 and gammadelta T-cells in parasitic infection, chickens were orally infected with Eimeria acervulina and the expression of IL-2 mRNA transcripts in the spleen and duodenum and the percentage of gammadelta T-cells in the duodenum were examined. Following both, the primary and secondary infections, a significant enhancement of IL-2 mRNA transcripts in the spleen and intestine and increased percentage of intraepithelial gammadelta T-cells in the duodenum were observed. These results indicate that host immune responses to E. acervulina involve an up-regulation of IL-2 secretion and an increased duodenum gammadelta T-cells.
Choi
K D
KD
Immunology and Disease Resistance Laboratory, Livestock and Poultry Sciences Institute, Building 1040, BARC-East, USDA, Beltsville, MD 207O5, USA.
Lillehoj
H S
HS
D000818:Animals; D002645:Chickens / Q000276:immunology*; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary*; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D017931:DNA Primers / Q000737:chemistry; D004386:Duodenum / Q000276:immunology*; D004539:Eimeria; D005434:Flow Cytometry / Q000662:veterinary; D007371:Interferon-gamma / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology* / Q000469:parasitology; D012333:RNA, Messenger / Q000096:biosynthesis*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D015854:Up-Regulation
D017931:DNA Primers; D007376:Interleukin-2; D012333:RNA, Messenger; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011994:Recombinant Proteins; D003208:Concanavalin A; D007371:Interferon-gamma