A novel thermophilic glycosynthase that effects branching glycosylation.
A novel thermophilic glycosynthase that effects branching glycosylation has been obtained by mutation of the nucleophile in the active site of the glycosidase from Sulfolobus solfataricus. Two methods for the use of this mutant are reported.
Trincone
A
A
Istituto per la Chimica di Molecole di Interesse Biologico, C.N.R., Naples, Italy. atrincone@icmib.na.cnr.it
Perugino
G
G
Rossi
M
M
Moracci
M
M
D019943:Amino Acid Substitution; D001665:Binding Sites / Q000235:genetics; D002384:Catalysis; D005947:Glucose / Q000031:analogs & derivatives / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D006031:Glycosylation / Q000187:drug effects; D006358:Hot Temperature; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D016944:Sulfolobus / Q000201:enzymology
C030364:glucosyl fluoride; D006026:Glycoside Hydrolases; D005947:Glucose