Characterization of two members of the maize gene family, Incw3 and Incw4, encoding cell-wall invertases.
Two maize putative cell-wall invertase genes (Incw3 and Incw4) have been isolated by screening a genomic DNA library (Zea mays L. W22) using the cDNA probes encoding the two maize cell-wall invertases Incw1 and Incw2. The Incw3 and Incw4 genes contain six exons/five introns and five exons/four introns, respectively. The protein sequences deduced from both genes revealed a beta-fructosidase motif and a cysteine catalytic site known to be conserved in invertase genes. A detailed analysis of the protein and nucleotide sequences provides evidence that the Incw3 and the Incw4 genes encode putative cell-wall invertases. Furthermore, the isoelectric point deduced from the INCW4 protein sequence suggested that the Incw4 gene may encode a unique type of cell-wall invertase unbound in the apoplast. Gene expression studies using RT-PCR and in-situ RT-PCR hybridization showed that the Incw3 expression is organ/tissue-specific and developmentally regulated. In contrast, the Incw4 gene is constitutively expressed in all vegetative and reproductive tissues tested.
Kim
J Y
JY
Laboratoire Structure et Métabolisme des Plantes, Institut de Biotechnologie des Plantes, CNRS-UMR 8618, Bâtiment 630, Université de Paris-Sud (XI), 91405, Orsay, France.
Mahé
A
A
Guy
S
S
Brangeon
J
J
Roche
O
O
Chourey
P S
PS
Prioul
J L
JL
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D002473:Cell Wall / Q000201:enzymology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics*; D017403:In Situ Hybridization; D007438:Introns; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D003313:Zea mays / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D043324:beta-Fructofuranosidase
D018744:DNA, Plant; D007527:Isoenzymes; D018749:RNA, Plant; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase