Inhibitory effect of sanguiin H-11 on chemotaxis of neutrophil.
Neutrophil is accumulated by chemoattractants at inflammatory sites. In order to find a new substance to regulate the chemotaxis of neutrophil, we examined the effects of purified tannins and related compounds (33 species). Among those studied, sanguiin H-11, purified from the plant Sanguisorba officinalis, was the most potent inhibitor of rat cytokine induced neutrophil chemoattractant-1 (CINC-1, a counterpart of human GRO (melanoma growth-stimulating activity)) dependent chemotaxis in rat neutrophils. Sanguiin H-11 inhibited platelet-activating factor- and N-formylmethionyl-leucyl-phenylalanine-dependent chemotaxis of neutrophil in addition to CINC-1-dependent reactions. Sanguiin H-11 also inhibited chemokinesis of neutrophil, but did not drastically affect the increase of intracellular free [Ca2+] level or degranulation monitored by the secretion of elastase, following chemoattractant stimulation. These results indicated that sanguiin H-11 is a potent inhibitor of chemoattractant-dependent and independent neutrophil movement.
Konishi
K
K
Department of Microbiology, Nippon Dental University School of Dentistry at Tokyo, Japan.
Urada
M
M
Adachi
I
I
Tanaka
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D015550:Cell Degranulation / Q000187:drug effects; D018932:Chemokine CCL2 / Q000302:isolation & purification / Q000494:pharmacology; D054360:Chemokine CXCL1; D018925:Chemokines / Q000494:pharmacology*; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000302:isolation & purification / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology; D047348:Hydrolyzable Tannins; D036341:Intercellular Signaling Peptides and Proteins; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification; D013634:Tannins / Q000494:pharmacology*
C475829:CXCL1 protein, human; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C513297:Cxcl1 protein, rat; D006133:Growth Substances; D047348:Hydrolyzable Tannins; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D013634:Tannins; C406326:sanguiin H 11; D010196:Pancreatic Elastase; D002118:Calcium