title: Hematopoietic reconstitution after lethal irradiation and bone marrow transplantation: effects of different hematopoietic cytokines on the recovery of thymus, spleen and blood cells.
abstract: Lethally irradiated mice were grafted with syngeneic bone marrow cells or left ungrafted. Mice of each group were injected with different hematopoietic cytokines for 5 consecutive days starting immediately after irradiation or left uninjected. The recovery of lymphoid tissues induced by hematopoietic cytokines 7 days after irradiation and bone marrow cell transplantation was comparable to that observed at days 21-28 in irradiated, bone marrow-grafted, but cytokine-uninjected mice. IL-11 or IL-6, in combination with IL-3, was able to hasten thymus, spleen and blood cell numbers and functions. SCF also displayed a detectable effect when used with IL-3. Conversely, the IL-6 superagonist K-7/D-6 was able, when injected alone, to induce significant recovery of thymus, spleen and blood cells. Thus, K-7/D-6 appears to be a most efficient cytokine for fast reconstitution of lymphoid tissues after irradiation and bone marrow transplantation.
authors.affiliation: Laboratory of Immunology, AMB-PRO-TOSS, ENEA CR Casaccia, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Frasca
authors.forename: F
authors.initials: F
authors.lastname: Guidi
authors.forename: M
authors.initials: M
authors.lastname: Arbitrio
authors.forename: C
authors.initials: C
authors.lastname: Pioli
authors.forename: F
authors.initials: F
authors.lastname: Poccia
authors.forename: R
authors.initials: R
authors.lastname: Cicconi
authors.forename: G
authors.initials: G
authors.lastname: Doria
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000473:pathology / Q000502:physiology; D016026:Bone Marrow Transplantation; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D006410:Hematopoiesis; D017370:Interleukin-11 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000503:physiopathology; D013950:Thymus Gland / Q000503:physiopathology; D014185:Transplantation, Isogeneic; D014916:Whole-Body Irradiation
chemical_list: D017370:Interleukin-11; D007377:Interleukin-3; D015850:Interleukin-6