Resolution of the 1H-1H NOE spectrum of RNA into three dimensions using 15N-1H two-bond couplings.
The feasibility of using two-bond 15N-1H couplings to resolve the 1H-1H nuclear Overhauser effect spectrum of RNA into a third dimension was investigated, using the 36-nucleotide gene 32 messenger RNA pseudoknot of bacteriophage T2 as an example. The two-bond 15N-1H couplings present in adenosine and guanosine were found to be suitable for generating a three-dimensional 1H-1H-15N NOESY-HSQC spectrum with reasonably good sensitivity, as well as favorable chemical shift dispersion in the nitrogen dimension. The described NMR experiment provides a tool that can be used to complement other heteronuclear methods in the analysis of RNA structure.
Hoffman
D W
DW
Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, University of Texas at Austin, 78712, USA. dhoffman@mail.utexas.edu
D004926:Escherichia coli / Q000235:genetics; D006859:Hydrogen; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000235:genetics; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000648:ultrastructure; D012317:RNA Phages / Q000737:chemistry; D012329:RNA, Bacterial / Q000737:chemistry
D009587:Nitrogen Isotopes; D009711:Nucleotides; D012329:RNA, Bacterial; D012313:RNA; D006859:Hydrogen