title: Ultraviolet spectrometric determination of neutral monosaccharides by HPLC with ethanolamine.
abstract: The reaction between ethanolamine and sugars in a borate solution gave intense UV absorption at 310 nm. By adding ethanolamine in the mobile phase, simple and sensitive ion-exchange HPLC of sugar-borate complexes was achieved. This method can be used to measure as little as 100 pmole of seven monosaccharides from pectic polysaccharides.
authors.affiliation: Hannan R&D Center, Fuji Oil Co. Ltd., Osaka, Japan. 940080@so.fujioil.co.jp
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: C
authors.initials: C
authors.lastname: Hatanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Nagamatsu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019856:Ethanolamine; D009005:Monosaccharides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D009005:Monosaccharides; D019856:Ethanolamine