Adaptation of an insect cell line of Spodoptera frugiperda to grow at 37 degrees C: characterization of an endodiploid clone.
Sf21 and Sf9 cell lines established from the lepidoptera Spodoptera frugiperda do not display major induction of heat shock proteins when exposed to a temperature of 37 degrees C. After some months of adaptation at 37 degrees C we obtained two new cell lines, Sf21-HT and Sf9-HT, which have now been established for several years in our laboratory. The Sf9-HT line displays a slightly shorter doubling time at 37 degrees C than the wild type at 28 degrees C, but cell lethality gives rise to an earlier growth arrest. The composition of total lipid extract from heat-adapted cells reveals a higher sphingomyelin to phosphatidylcholine ratio and a higher percentage of saturated fatty acids, which are expected for the lower membrane fluidity, required for thermotolerance. The cell volume of Sf9-HT is doubled, and by flow cytometry we showed that the DNA content is twice that in the parental cell line. Karyotypic examination of metaphasic cells achieved under epifluorescence microscopy revealed a doubled chromosome number in Sf9-HT.
Gerbal
M
M
Laboratoire de Pathologie Coanparée, INRA-CNRS URA 2209, Saint-Christol-lèz-Alès, France.
Fournier
P
P
Barry
P
P
Mariller
M
M
Odier
F
F
Devauchelle
G
G
Duonor-Cerutti
M
M
D000818:Animals; D002455:Cell Division; D002460:Cell Line / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004171:Diploidy; D005434:Flow Cytometry; D006360:Heat-Shock Proteins / Q000378:metabolism; D006358:Hot Temperature; D050356:Lipid Metabolism; D020401:Primed In Situ Labeling; D018411:Spodoptera / Q000166:cytology*
D006360:Heat-Shock Proteins; D004247:DNA; D002784:Cholesterol