title: Characterization of P1-deficient isogenic mutant of Haemophilus influenzae biogroup aegyptius associated with Brazilian purpuric fever.
abstract: Haemophilus influenzae biogroup aegyptius (formerly H. aegyptius) is the etiologic agent of Brazilian purpuric fever (BPF). A surface-exposed epitope on the outer membrane protein P1 is present on most strains of H. influenzae biogroup aegyptius associated with BPF but is absent in almost all non-disease associated strains. The role of the outer membrane protein P1 in the pathogenesis of this disease was evaluated by utilizing an isogenic P1-deficient mutant. We compared the ability of the wild type and P1 isogenic mutant to grow under various conditions. The P1-deficient strain grew at a similar rate to the wild type in both complex and chemically defined medium. The P1-deficient mutant also had a similar growth rate to the wild type under anaerobic conditions. Anaerobic growth, however, resulted in up-regulation of the P1 protein in the wild type strain. Three assays were used to examine the pathophysiologic role of the P1 protein in BPF: 1) serum resistance; 2) sustained bacteremia in the infant rat model; and 3) the human microvascular endothelial cell (HMEC) cytotoxicity assay. Both the mutant and wild-type strains were resistant to killing in 95% normal human serum. The P1-deficient strain was also as virulent as the wild type in both the infant rat model of bacteremia and in the HMEC-1 tissue culture model. These results demonstrate that serum resistance, sustained bacteremia in the infant rat, and cytotoxicity of HMEC cells occur in the absence of P1. The P1 protein is not essential for the pathogenic potential identified by these assays. However, these results demonstrate that an anaerobic environment is a potent physiologic regulator of P1 protein expression. The impact of anaerobiosis on protein expression and pathogenesis will require further investigations.
authors.affiliation: Department of Microbiology, State University of New York at Buffalo, New York, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Segada
authors.forename: G M
authors.initials: GM
authors.lastname: Carlone
authors.forename: L L
authors.initials: LL
authors.lastname: Gheesling
authors.forename: A J
authors.initials: AJ
authors.lastname: Lesse
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D000831:Animals, Newborn; D016470:Bacteremia / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001938:Brazil; D002460:Cell Line; D003470:Culture Media; D003603:Cytotoxins / Q000235:genetics; D004730:Endothelium, Vascular / Q000187:drug effects; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D006801:Humans; D015151:Immunoblotting; D009154:Mutation; D051381:Rats; D015610:Serum Bactericidal Test; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D003603:Cytotoxins; C067559:outer membrane protein P1, Haemophilus influenzae type b