title: Attenuation of luminol-amplified chemiluminescent intensity and lipid peroxidation in the livers of quercetin-fed mice.
abstract: We assessed the antioxidative effects of quercetin-feeding on ddY strain male mice by measuring luminol-amplified chemiluminescence that was enhanced by a hydrophilic free radical initiator 2, 2'-azobis(2-amidinopropane) dihydrochloride. Quercetin suppressed chemiluminescent intensity in a dose-dependent manner in vitro with a half-inhibition concentration (IC(50)) of 3x10(-8) M, which was lower than the value of synthetic antioxidant 2, 6-di-tert-butyl-p-cresol (IC(50): 7x10(-7) M). Lysosomal (12000xg pellet) and supernatant fractions obtained from the livers of mice fed a diet containing 0.2% quercetin for 7 days showed more inhibition of chemiluminescent intensity than those from non-treated mice. Quercetin feeding also resulted in the inhibition of lipid peroxidation as determined by a thiobarbituric acid reactive substance test which detected suppression of the release of lysosomal enzymes induced by lipophilic free radical initiator 2, 2'-azobis(2,4-dimethylvaleronitrile). These results probably reflect radical quenching activity of quercetin, indicating that the measurement of chemiluminescence is a useful tool for the assessment of total antioxidant activity in biological systems.
authors.affiliation: Department of Food Science, Kyoto Women's University, Higashiyama-ku, Kyoto, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawagoe
authors.forename: K
authors.initials: K
authors.lastname: Nakagawa
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000284:Administration, Oral; D000578:Amidines / Q000494:pharmacology; D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009570:Nitriles / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D011794:Quercetin / Q000008:administration & dosage*; D013347:Subcellular Fractions / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000578:Amidines; D001391:Azo Compounds; D009570:Nitriles; D016877:Oxidants; D010636:Phenols; D017392:Thiobarbituric Acid Reactive Substances; C055792:2,2'-azobis(2,4-dimethylvaleronitrile); D002084:Butylated Hydroxytoluene; D008165:Luminol; C046728:2,2'-azobis(2-amidinopropane); D011794:Quercetin; D000135:Acid Phosphatase; D000118:Acetylglucosaminidase