Enzymatically modified ion-selective electrodes for flow injection analysis.
The application of potentiometric biosensors based on membrane ion-selective electrodes for flow injection analysis (FIA) is presented. The biosensors have been obtained by covalent binding of enzyme molecules directly to surface of ion-selective membranes. The biosensor/FIA systems enable the determination of urea and penicillin in the millimolar ranges of concentration. About 30 samples per hour can be analysed in the performed FIA systems. The operational stability of the bioanalytical systems exceeds 1 month.
Koncki
R
R
Department of Chemistry, University of Warsaw, Poland. rkoncki@chem.uw.edu.pl
Wałcerz
I
I
Leszczyńska
E
E
D015374:Biosensing Techniques / Q000295:instrumentation*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D017022:Flow Injection Analysis / Q000295:instrumentation*; D017736:Ion-Selective Electrodes; D008567:Membranes, Artificial; D010405:Penicillinase / Q000737:chemistry / Q000378:metabolism; D010406:Penicillins / Q000032:analysis*; D011199:Potentiometry / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014508:Urea / Q000032:analysis*; D014510:Urease / Q000737:chemistry / Q000378:metabolism
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010406:Penicillins; D014508:Urea; D014510:Urease; D010405:Penicillinase