Optimization of the extraction of polycyclic aromatic hydrocarbons from wood samples by the use of microwave energy.
A simple and rapid microwave-assisted extraction (MAE) procedure was developed and optimized for benzo[a]anthracene, benzo[e]pyrene, benzo[b]fluoranthene, benzo[k]fluoranthene and benzo[a]pyrene in wood samples. The spiked wood used was prepared 3 months before analysis to simulate weathering processes and to allow the formation of analyte-matrix interaction. The samples, immersed in acetonitrile were irradiated with microwaves in a closed-vessel system. Optimization of the method was achieved by using a factorial design approach on parameters such as extraction time, temperature and sample amount. The analysis of extracts has been carried out by reversed-phase high-performance liquid chromatography with fluorescence detection for quantification and UV-diode-array detection for confirmation. The MAE procedure yielded extracts that could be analyzed directly without any preliminary clean-up or solvent exchange steps.
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Departamento de Química Analítica, Nutrición y Bromatología, Facultad de Química, Universidad de Santiago de Compostela, Spain.
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D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008872:Microwaves; D011083:Polycyclic Compounds / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D014934:Wood
D011083:Polycyclic Compounds