title: Constitutive expression of the UGA4 gene in Saccharomyces cerevisiae depends on two positive-acting proteins, Uga3p and Uga35p.
abstract: The first specific precursor of porphyrin biosynthesis is delta-aminolevulinic acid. delta-Aminolevulinic acid enters Saccharomyces cerevisiae cells through the gamma-aminobutyric acid specific permease Uga4p. It was described that this permease is inducible by gamma-aminobutyric acid and its regulation involves several specific and pleiotropic transcriptional factors. However, some studies showed that under certain growth conditions the synthesis of Uga4p was not dependent on the presence of gamma-aminobutyric acid. To study the effect of the trans-acting factors Uga43p, Uga3p, Uga35p, Ure2p and Gln3p on the expression of UGA4, we measured gamma-aminobutyric acid and delta-aminolevulinic acid uptake in yeast mutant cells, lacking one of these regulatory factors, grown under different conditions. Experiments analyzing the UGA4 promoter using a fusion construction UGA4::lacZ were also carried out. The results show that the constitutive expression of the UGA4 gene found in cells under certain growth conditions depends on the presence of Uga3p and Uga35p. In contrast, Gln3p and Ure2p do not seem to have any effect on this constitutive mechanism.
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias. CIPYP (CONICET, FCEyN, UBA), Ciudad Universitaria, Pabellón II, 2o Piso, 1428, Buenos Aires, Argentina.
authors.forename: S C
authors.initials: SC
authors.lastname: Garcia
authors.forename: M B
authors.initials: MB
authors.lastname: Moretti
authors.forename: A
authors.initials: A
authors.lastname: Batlle
mesh_terms: D000409:Alanine / Q000378:metabolism; D000622:Aminolevulinic Acid / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D050485:GABA Plasma Membrane Transport Proteins; D015966:Gene Expression Regulation, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D027361:Organic Anion Transporters; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: C067023:DAL81 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D050485:GABA Plasma Membrane Transport Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C092661:UGA3 protein, S cerevisiae; C513599:UGA4 protein, S cerevisiae; D005680:gamma-Aminobutyric Acid; D000622:Aminolevulinic Acid; D001616:beta-Galactosidase; D000409:Alanine