Homologous and heterologous interference requires bovine herpesvirus-1 glycoprotein D at the cell surface during virus entry.
Expression of glycoprotein D (gD) of alphaherpesviruses protects cells from superinfection by homologous and heterologous viruses by a mechanism termed interference. We recently showed that MDBK cells expressing bovine herpesvirus (BHV)-1gD (MDBK(gD)) resist BHV-1, pseudorabies virus (PRV) and herpes simplex virus-1 (HSV-1) but not the more closely related BHV-5 infection as determined by the number of plaques produced. However, the plaque size is reduced in all four viral infections suggesting a block in cell-to-cell transmission. Here, we show that MDBK cells expressing truncated BHV-1 gD, designated MDBK(t-gD), secreted soluble gD and were fully susceptible to infection by all the four viruses when the cells were washed prior to infection. When MDBK cells or MDBK(t-gD) cells were treated with medium containing truncated gD prior to infection, they partially resisted BHV-1, PRV and HSV-1 but not BHV-5. Interestingly, both BHV-1 and BHV-5 formed normal-sized plaques in MDBK(t-gD) cells suggesting that the viruses were able to spread efficiently. Thus BHV-1 gD is required at the cell surface at the time of infection in order to block BHV-1, HSV-1 and PRV infections, consistent with a common coreceptor for the three gDs.
Dasika
G K
GK
Animal Health and Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, Madison, WI 53706, USA. Dasika@uthscsa.edu
Letchworth
G J
GJ
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D005822:Genetic Vectors; D006566:Herpesviridae Infections / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D008969:Molecular Sequence Data; D014162:Transfection; D014764:Viral Proteins / Q000502:physiology*; D014779:Virus Replication
D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins