Beta-lactamase activity of anaerobic bacteroides strains isolated from clinical samples and their susceptibility to antimicrobial agents.
Beta-lactamase production and susceptibility to 13 antimicrobial agents (penicillin-G, amoxycillin, amoxycillin/clavulanic acid, cefoxitin, imipenem, clindamycin, metronidazole, piperacillin, ticarcillin, rifampicin, chloramphenicol, tetracycline and erythromycin) of 32 isolated Bacteroides strains were determined. The strains included 23 isolates of B. frugilis, 2 B. thetaioatomicron, 2 B. ovatus, 1 B. distasonis, 1 B. capillosus, 1 B. uniformis, 1 B. ureolyticus and 1 B. merdae. beta-Lactamase production was detected in 65% of the Bacteroides using the nitrocefin test, All the antibiotic agents showed excellent activity against beta-lactamase negative strains (for tetracycline, ticarcillin and clindamycin, 90% were susceptible, whereas for the other drugs, 100% were susceptible). beta-Lactamase-positive Bacteroides strains showed 95% susceptibility to metronidazole and rifampicin; 90% susceptibility to piperacillin and cefoxitin; 85% susceptibility to tetracycline and erythromycin; 80% susceptibility to clindamycin and amoxycillin/clavulanic acid, and 76% susceptibility to ticarcillin. All beta-lactamase-positive strains were found to be susceptible to imipenem and chloramphenicol.
Uraz
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Microbiology Department, Faculty of Science and Arts Gazi University, Ankara, Turkey.
Türkmen
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D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001439:Bacteroides / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000706:statistics & numerical data; D001618:beta-Lactamases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D001618:beta-Lactamases