The retinoblastoma-interacting zinc-finger protein RIZ is a downstream effector of estrogen action.
Co-immunoprecipitation experiments in cell extract from cultured cells or target tissues indicated that estrogen receptor was complexed with the retinoblastoma binding protein RIZ in a ligand-dependent manner. Mapping of interaction sites indicated that in both proteins the same regions and motifs responsible for the interaction of transcriptional co-activator and nuclear receptors were involved. In cultured cells, estradiol induced a redistribution of RIZ protein within the nucleus and in the cytoplasm. A similar effect was produced in vivo, in prepuberal rat endometrium, by administration of a physiological dose of estradiol. Therefore, RIZ protein could be a specific effector of estrogen action downstream of the hormone-receptor interaction, presumably involved in proliferation control.
Abbondanza
C
C
Istituto di Patologia generale ed Oncologia, Seconda Università degli studi di Napoli, Larghetto Sant'Aniello a Caponapoli 2, I-80138 Naples, Italy.
Medici
N
N
Nigro
V
V
Rossi
V
V
Gallo
L
L
Piluso
G
G
Belsito
A
A
Roscigno
A
A
Bontempo
P
P
Puca
A A
AA
Molinari
A M
AM
Moncharmont
B
B
Puca
G A
GA
D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004268:DNA-Binding Proteins; D004967:Estrogens / Q000502:physiology*; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism; D014157:Transcription Factors; D016335:Zinc Fingers
D017931:DNA Primers; D004268:DNA-Binding Proteins; D004967:Estrogens; D009687:Nuclear Proteins; D011960:Receptors, Estrogen; D014157:Transcription Factors; D011495:Histone-Lysine N-Methyltransferase; C093563:PRDM2 protein, human