Development of a plasmid vector for easy selection of strong promoters.
A promoter vector pACPR33 for Escherichia coli based on the promotorless ampicillin-resistance gene from pBR322 has been constructed. The promoter of the ampicillin-resistance gene was deleted and replaced by a suitable multiple cloning site. Molecular cloning of promoters into the polylinker resulted in activation of the ampicillin resistance in E. coli. The plasmid contains a functional origin of DNA replication and a tetracycline resistance gene for E. coli, and a chloramphenicol resistance gene for S. aureus. The vector permitted direct detection of promoter activity, especially strong promoters, by easy iodometric determination of beta-lactamase activity in liquid or solid media.
Piñeiro
S A
SA
Laboratorio BioSidus S.A., (1254) Constitución 4234, Buenos Aires, Argentina.
Sordelli
D O
DO
Centrón
D
D
D000668:Ampicillin Resistance / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics*; D011401:Promoter Regions, Genetic; D001618:beta-Lactamases / Q000378:metabolism
D001618:beta-Lactamases