Dictyostelium amoebae lacking an F-box protein form spores rather than stalk in chimeras with wild type.
Using a selection for Dictyostelium mutants that preferentially form spores, we have recovered a mutant called CheaterA. In chimeras with isogenic wild-type cells, the CheaterA mutant preferentially forms viable spores rather than inviable stalk cells. The mutant causes wild-type cells that have begun to express spore-specific genes to accumulate in the prestalk compartment of the developing organism. In the wild-type cells, the chtA transcript is absent in growing cells and appears early in development. No transcript was detected in the mutant by Northern blot. The chtA gene codes for a protein with an F-box and WD40 domains. This class of protein usually forms part of an Skp1, cullin, F-box (SCF) complex that targets specific protein substrates for ubiquitination and degradation.
Ennis
H L
HL
Department of Anatomy and Cell Biology, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
Dao
D N
DN
Pukatzki
S U
SU
Kessin
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002678:Chimera; D017931:DNA Primers; D004023:Dictyostelium / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D044783:F-Box Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D015800:Protozoan Proteins; D013172:Spores, Fungal
D002352:Carrier Proteins; C406731:ChtA protein, Dictyostelium; D017931:DNA Primers; D044783:F-Box Proteins; D015800:Protozoan Proteins