Genetic analysis of SecA-SecY interaction required for spore development in Bacillus subtilis.
All spontaneous suppressor mutations obtained from a secA12 sporulation-defective mutant in Bacillus subtilis were localized in highly conserved membrane-spanning regions of SecY. The expression of early sporulation genes, kinA and spo0A encoding a histidine kinase and a transcription regulator for several sporulation genes, respectively, was restored in these suppressor mutants. These results indicate that the secretion function of translocase combined with Sec proteins is required for sporulation in B. subtilis.
Kobayashi
H
H
Laboratory of Molecular Genetics, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo, Japan.
Ohashi
Y
Y
Nanamiya
H
H
Asai
K
K
Kawamura
F
F
D018528:ATP-Binding Cassette Transporters; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins; D009154:Mutation; D010675:Pheromones / Q000378:metabolism; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D015398:Signal Transduction; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D010675:Pheromones; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli; C087737:Spo0H protein, Bacillus subtilis; D014157:Transcription Factors; C085800:modF protein, E coli; D001616:beta-Galactosidase; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins