Characterization of an avidin-bonded column for direct injection in reversed-phase high-performance liquid chromatography.
A denatured avidin-bonded column was suitable for use in the reversed-phase HPLC and complete recovery of serum protein over a wide range of pH. Retention property of the denatured avidin-bonded column was very nearly to a non-denatured avidin-bonded column already reported and, however, showed very high stability to organic solvent. A performance of the denatured avidin-bonded column was maintained even if 500 continuous injections of human serum (total 10 ml).
Tanaka
H
H
GL Sciences, Saitama, Japan. rd@gls.co.jp
Takahashi
K
K
Ohira
M
M
D001360:Avidin / Q000737:chemistry*; D001769:Blood; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration
D002021:Buffers; D001360:Avidin