title: Doxapram accentuates white matter injury in neonatal rats following bilateral carotid artery occlusion.
abstract: The effect of the respiratory stimulant, doxapram, on white matter damage was investigated in neonatal rats under cerebral ischemia. Five-day-old rats underwent bilateral carotid artery occlusion with or without 50 mg/kg i.p. of doxapram. Their brains were neuropathologically examined 48 h later. Doxapram induced about a 20% decrease of PCO(2) for 90 min, but did not cause any neuropathological abnormalities. Bilateral carotid artery occlusion resulted in mild cerebrocortical lesions in 67% of pups, and white matter lesions in the internal capsule in 44%. Doxapram, in addition to bilateral carotid artery occlusion, produced more severe white matter injury in the internal capsule (injury score; 0.67+/-0.87 vs. 1.70+/-0.48, P<0.05) and in the subcortical white matter (0.33+/-0. 67 vs. 1.10+/-0.54, P<0.05). These results demonstrated that the use of doxapram under an ischemic condition accentuates white matter damage in neonatal rats.
authors.affiliation: Department of Pediatrics, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Uehara
authors.forename: H
authors.initials: H
authors.lastname: Yoshioka
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: R
authors.initials: R
authors.lastname: Ochiai
authors.forename: T
authors.initials: T
authors.lastname: Naito
authors.forename: K
authors.initials: K
authors.lastname: Hasegawa
authors.forename: T
authors.initials: T
authors.lastname: Sawada
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001157:Arterial Occlusive Diseases / Q000150:complications*; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D002339:Carotid Arteries / Q000473:pathology*; D004315:Doxapram / Q000009:adverse effects*; D006801:Humans; D007231:Infant, Newborn; D007969:Leukomalacia, Periventricular / Q000209:etiology / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D019141:Respiratory System Agents / Q000009:adverse effects*
chemical_list: D019141:Respiratory System Agents; D004315:Doxapram