Role of hyperbaric oxygen exposure in reduction of lipid peroxidation and in multiple organ failure induced by zymosan administration in the rat.
The aim of the present study was to evaluate the effects of hyperbaric oxygen (HBO) therapy on multiple organ failure induced by zymosan. Administration of zymosan (500 mg/kg) in the rat induced neutrophil infiltration in the lung, liver, and intestine as evaluated by increase in myeloperoxidase (MPO) activity. Therefore, lipid peroxidation was significantly increased in zymosan-treated rats. This inflammatory process coincided with the damage of lung, liver, and small intestine. Immunohistochemical examination demonstrated a marked increase in the immunoreactivity to nitrotyrosine in the lung, liver, and small intestine of zymosan-shocked rats. HBO (2 absolute Atmosphere) exposure attenuates the increase in the tissue levels of MPO and malondialdehyde (MDA) caused by zymosan in the lung, liver, and intestine. In addition, HBO (2 absolute Atmosphere) was effective in preventing the development of lung, liver, and intestine injury. Taken together, the present results demonstrate that HBO may also be an efficacious treatment in multiple organ failure induced by zymosan.
Cuzzocrea
S
S
Institute of Pharmacology, University of Messina, Italy.
Imperatore
F
F
Costantino
G
G
Luongo
C
C
Mazzon
E
E
Scafuro
M A
MA
Mangoni
G
G
Caputi
A P
AP
Rossi
F
F
Filippelli
A
A
D000818:Animals; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009102:Multiple Organ Failure / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D010538:Peritonitis / Q000139:chemically induced / Q000150:complications; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D015054:Zymosan / Q000633:toxicity
C002744:3-nitrotyrosine; D014443:Tyrosine; D008315:Malondialdehyde; D015054:Zymosan; D009195:Peroxidase