Allogeneic class I major histocompatibility complex gene transfer in murine neuroblastoma in vivo.
In vivo intratumoral gene transfer of allogeneic class I major histocompatibility complex (MHC) genes augments the immune response against weak tumor antigens. In this study, mice inoculated with the allogeneic MHC molecule (H-2Kb), had transduced-murine neuroblastoma C1300S3 cells showed prolonged survival relative to non-transduced or neo transduced tumors (p < 0.005). Interestingly, direct in vivo gene transfer of H-2Kb plasmid DNA complexed with HVJ-liposomes into S3 tumors was highly efficient, resulting in transduction of 8% of the interstitial cells within the tumor but rarely within tumor cells. Regression of established tumors and prolonged survival occurred in 50% of mice injected with H-2Kb, in contrast to no tumor regression in mice receiving control plasmid (p < 0.005). This study concludes that interstitial cells could serve as an important target of intratumoral gene transfer, and further that HVJ-liposome complexes could be a vehicle for in vivo gene transfer.
Ishida
H
H
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Kaneda
Y
Y
Yamane
S
S
Sawada
T
T
Plautz
G E
GE
Matsumura
T
T
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005805:Genes, MHC Class I; D008081:Liposomes; D051379:Mice; D009447:Neuroblastoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D008081:Liposomes