Structural organization of the human glutathione reductase gene: determination of correct cDNA sequence and identification of a mitochondrial leader sequence.
The primary structure of human glutathione reductase gene (GSR) was determined by genomic cloning. The gene structure of human GSR spans 50 kb, consists of 13 exons, and was found to be highly similar to the mouse GSR gene. The coding sequence of human GSR resides on all 13 exons. An N-terminal arginine-rich mitochondrial leader sequence was present, with high homology to the murine leader sequence, between two in-frame start codons in the first exon. The 5' and 3' intron/exon splice junctions, with one exception, followed the general consensus sequences for intron spliced donor and acceptance sites.
Kelner
M J
MJ
Department of Pathology, University of California, San Diego, 200 West Arbor Drive, San Diego, California, 92103-8320, USA. mkelner@ucsd.edu
Montoya
M A
MA
D000818:Animals; D001483:Base Sequence; D018387:Codon, Initiator; D004247:DNA; D018076:DNA, Complementary; D005091:Exons; D005980:Glutathione Reductase / Q000235:genetics*; D006801:Humans; D007438:Introns; D051379:Mice; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics*; D015183:Restriction Mapping
D018387:Codon, Initiator; D018076:DNA, Complementary; D021382:Protein Sorting Signals; D004247:DNA; D005980:Glutathione Reductase