A somatic gene transfer approach using recombinant fusion proteins to map muscle-motoneuron projections in Xenopus spinal cord.
A combination of somatic gene transfer with fusion protein technology has been developed, thus providing an innovative means of mapping muscle-motoneuronal connections in Xenopus tadpole spinal cord. We analyzed whether a neuronal tracer created by the fusion of the LacZ gene to the tetanus toxin C fragment (LacZ-TTC) could be produced from plasmid DNA injected into muscle, and whether it could be released and undergo retrograde transport into motoneurons. Plasmids encoding various fusion protein constructions, with or without a signal peptide, were injected into dorsal or caudal muscles of premetamorphic tadpoles. The marker was produced in the muscle at constantly high levels. At one month post-injection, the fusion protein passed the neuromuscular junction and underwent retrograde transport into motoneurons. Transfer into motoneurons was seen for every animal injected, emphasizing the high reproducibility and efficiency of the process. No uptake of beta-gal protein into motoneurons was observed in the absence of the TTC fragment. Furthermore, no enhancement was obtained by adding a signal peptide. These results provide the first demonstration of the synthesis and transport of a TTC fusion protein produced directly from exogenous DNA in a vertebrate system.
Coen
L
L
Laboratoire de Physiologie Générale et Comparée, UMR 8572 CNRS, Muséum National d'Histoire Naturelle, Paris, France.
Kissa
K
K
le Mevel
S
S
Brulet
P
P
Demeneix
B A
BA
D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active; D004274:DNA, Recombinant / Q000235:genetics; D018014:Gene Transfer Techniques; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000166:cytology*; D009132:Muscles / Q000294:innervation / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013116:Spinal Cord / Q000254:growth & development*; D014982:Xenopus laevis / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins