Control of column temperature in reversed-phase liquid chromatography.
When separations by reversed-phase liquid chromatography (RP-LC) are carried out at temperatures other than ambient, resulting retention times and bandwidths can depend on the equipment used. As a result, an RP-LC separation that is adequate when carried out on one LC system may prove inadequate when the separation is repeated on a second system. In the present study, various temperature-related problems which can result in a failure of method transfer for non-ambient RP-LC methods were examined. Means for correcting for such effects and thereby ensuring method transferability are described.
Wolcott
R G
RG
Department of Chemistry, Linfield College, McMinnville, OR 97128, USA.
Dolan
J W
JW
Snyder
L R
LR
Bakalyar
S R
SR
Arnold
M A
MA
Nichols
J A
JA
D002853:Chromatography, Liquid / Q000379:methods*; D013696:Temperature