The in vitro hepatic microsomal metabolism of 3,5-dimethyl-4-(phenylazo)-(1H)-pyrazole in rats.
The in vitro hepatic microsomal metabolism of 3,5-dimethyl-4-(phenylazo)-(1H)-pyrazole (DMPAP) was studied using washed rat hepatic microsomal preparations fortified with NADPH. The substrate, DMPAP, and its potential metabolites, i.e. the corresponding reduction product, 3,5-dimethyl-4-amino-(1H)-pyrazole (DMAP), and the oxidation product, 3,5-dimethyl-4-(phenylazoxy)-(1H)-pyrazole (DMAPO), were synthesized and their structures elucidated by use of their spectral characteristics. DMPAP and its potential metabolites were then separated using a reverse phase HPLC system consisting of a C18 column and a mobile phase of acetonitrile:water (50:50) at a flow rate of 1 ml/min with UV detection at 254 nm. DMPAP was incubated with rat microsomal preparations, extracted into DCM in the presence of NaCl, and finally evaporated under a stream of nitrogen. The results from HPLC studies showed that DMPAP was metabolised to the corresponding reduction and oxidation products in the presence of NADPH.
Kaymakçioğlu
B
B
University of Marmara, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Istanbul, Turkey.
Oruç
E
E
Ulgen
M
M
Rollas
S
S
D000818:Animals; D001391:Azo Compounds / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D010084:Oxidation-Reduction; D011720:Pyrazoles / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D001391:Azo Compounds; D011720:Pyrazoles; D019255:NADPH Oxidases