Effects of interleukin-15 on in vitro human T cell proliferation and activation.
Interleukin-15 (IL-15) has been reported to have many activities on T cell populations, including a potential role in improving antigen-specific proliferation in HIV-1 disease. We tested this response in healthy adults by studying the response of T cell populations after stimulation with medium, tetanus, cytomegalovirus (CMV) antigens in cultures from 21 volunteers. IL-15 caused a dose-dependent increase in medium and antigen-induced proliferation. The expansion was due to CD8>natural killer (NK)>CD4 lymphocytes and memory > naive cells. The IL-15-stimulated CD8 cells had increased levels of the activation markers CD69 and DR. The published CMV-induced expression of CD57 on CD8+ cells was increased in CMV seronegative and seropositive subjects by IL-15. IL-15 appears to be a stimulator of T cell populations in healthy adults and may be useful in settings to enhance nonspecific NK activity or antigen-specific CD8 activity.
Hasan
M S
MS
Infectious Diseases Unit, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
Kallas
E G
EG
Thomas
E K
EK
Looney
J
J
Campbell
M
M
Evans
T G
TG
D000328:Adult; D000956:Antigens, Viral / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D019409:Interleukin-15 / Q000008:administration & dosage / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D013745:Tetanus Toxoid / Q000494:pharmacology
D000956:Antigens, Viral; D019409:Interleukin-15; D007376:Interleukin-2; D013745:Tetanus Toxoid