Immobilization of antibodies onto glass wool.
The immobilization of antibodies onto solid phases in an efficient and activity-retaining form is an important goal for both research and industry. Methods have been developed for the site-directed attachment of antibodies to agarose by oxidation of the carbohydrate moieties in their Fc region. Similar attachment to silianized supports have not been as successful. Here we describe a novel combination protocol for the site-directed attachment of periodate oxidized, goat polyclonal antibodies to glass wool fibers activated with 3-aminopropyltriethoxysilane. The study demonstrates that this procedure results in effective immobilization of polyclonal antibodies that retain their antigen-binding capacity. This protocol should prove useful in the development of more efficient and effective glass-based immunosupports.
Nisnevitch
M
M
E. Katzir Biotechnology Program, Research Institute, College of Judea and Samaria, Ariel, Israel.
Kolog-Gulco
M
M
Trombka
D
D
Green
B S
BS
Firer
M A
MA
D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D005898:Glass
D000906:Antibodies