title: Effect of environmental tobacco smoke on intracellular free calcium of boar sperm incubated in seminal plasma.
abstract: The extractable-respirable particulate of environmental tobacco smoke (ERP-ETS) was collected and tested for its effect on the intracellular free Ca(2+) concentration, [Ca(2+)](i), in boar sperm incubated in seminal plasma in vitro. The intracellular free Ca(2+) level is reportedly closely related to in sperm capacitation and acrosome reaction, which must take place to confer upon sperm the full fertilizing potential. In the absence of ERP-ETS [Ca(2+)](i) in the boar sperm increased dramatically from initially 75 nM to about 248 nM after a 5-h incubation in seminal plasma. However, such an increase was virtually inhibited by ERP-ETS at a concentration of 10 microg/ml, without any significant reduction of the sperm motility and viability. We conclude that application of ERP-ETS negatively affected the [Ca(2+)](i) of boar sperm in vitro.
authors.affiliation: Pesticide Research Center and Microbiology Department, Michigan State University, East Lansing, MI 48824, USA. zhourenq@pilot.msu.edu
authors.forename: R
authors.initials: R
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: S
authors.initials: S
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Haug
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002451:Cell Compartmentation; D002470:Cell Survival / Q000187:drug effects; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D016257:Fura-2 / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D012661:Semen / Q000166:cytology / Q000378:metabolism; D013075:Sperm Capacitation / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013552:Swine; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D005456:Fluorescent Dyes; D014028:Tobacco Smoke Pollution; C049925:fura-2-am; D002118:Calcium; D016257:Fura-2