title: The L17 ribosomal protein of Bacillus subtilis binds preferentially to curved DNA.
abstract: We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 respectively. The overproduction of L17 from B. subtilis in Escherichia coli was shown to have a strong effect on nucleoid morphology and segregation.
authors.affiliation: Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
authors.forename: M
authors.initials: M
authors.lastname: Zouine
authors.forename: C
authors.initials: C
authors.lastname: Beloin
authors.forename: C
authors.initials: C
authors.lastname: Ghelis
authors.forename: F
authors.initials: F
authors.lastname: Le Hégarat
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins; D002455:Cell Division / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C075779:Hbsu protein, bacteria; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C032493:ribosomal protein L17