title: Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization.
abstract: Animal studies and preliminary results in humans suggest that lower extremity and myocardial ischemia can be attenuated by treatment with angiogenic cytokines. The resident population of endothelial cells that is competent to respond to an available level of angiogenic growth factors, however, may potentially limit the extent to which cytokine supplementation enhances tissue neovascularization. Accordingly, we transplanted human endothelial progenitor cells (hEPCs) to athymic nude mice with hindlimb ischemia. Blood flow recovery and capillary density in the ischemic hindlimb were markedly improved, and the rate of limb loss was significantly reduced. Ex vivo expanded hEPCs may thus have utility as a "supply-side" strategy for therapeutic neovascularization.
authors.affiliation: Department of Medicine (Cardiovascular Research), St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA.
authors.forename: C
authors.initials: C
authors.lastname: Kalka
authors.forename: H
authors.initials: H
authors.lastname: Masuda
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: W M
authors.initials: WM
authors.lastname: Kalka-Moll
authors.forename: M
authors.initials: M
authors.lastname: Silver
authors.forename: M
authors.initials: M
authors.lastname: Kearney
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: J M
authors.initials: JM
authors.lastname: Isner
authors.forename: T
authors.initials: T
authors.lastname: Asahara
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008954:Models, Biological; D018919:Neovascularization, Physiologic