Structural studies of the O-specific polysaccharide from Plesiomonas shigelloides strain CNCTC 113/92.
The structure of the O-specific side chain of the lipopolysaccharide (LPS) of Plesiomonas shigelloides, strain CNCTC 113/92 has been investigated by NMR spectroscopy, matrix-assisted laser desorption/ionization time of flight mass spectrometry and sugar and methylation analysis. It was concluded that the polysaccharide is composed of a hexasaccharide repeating unit with the following structure: in which D-beta-D-Hepp is Dglycero-beta-Dmanno-heptopyranose and 6d-beta-D-Hep is 6-deoxy-beta-Dmanno-heptopyranose. This structure represents a novel hexasaccharide repeating unit of bacterial O-antigen that is characteristic and unique to the Plesiomonas shigelloides strain. Using the high-resolution magic angle spinning technique, 1H-NMR spectra were also obtained for the O-polysaccharide components of isolated LPS and in their original form directly on the surface of bacterial cells.
Czaja
J
J
L. Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland.
Jachymek
W
W
Niedziela
T
T
Lugowski
C
C
Aldova
E
E
Kenne
L
L
D002240:Carbohydrate Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000145:classification; D009844:Oligosaccharides / Q000737:chemistry*; D016981:Plesiomonas / Q000737:chemistry* / Q000145:classification; D012703:Serotyping; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D019081:O Antigens; D009844:Oligosaccharides