title: Transport studies of beta-lactam antibiotics and their degradation products across electrified water/oil interface.
abstract: An electrochemical method for quantifying beta-lactam antibiotics (cephalexin and ampicillin) and their hydrolysis products is described. Cyclic voltammetry at the water/nitrobenzene interface in a four-electrode system was used. The zwitterionic compounds were ionized to the necessary electrochemical form by pH adjustment. The pH change, however, resulted also in hydrolysis of the antibiotics. Hydrolysis products were characterized across UV-vis spectrum. The various hydrolysis products as well as the ionized antibiotics were studied in voltammetric transfer from water to nitrobenzene using the method of the interface between two immiscible electrolyte solutions (ITIES). It was concluded that this electrochemical method is suitable for the quantification of beta-lactam antibiotics and their hydrolysis products.
authors.affiliation: Departamento de Química Analítica e Inorgánica, Facultad de Ciencias Químicas, Universidad de Concepción, Chile.
authors.forename: L
authors.initials: L
authors.lastname: Basáez
authors.forename: P
authors.initials: P
authors.lastname: Vanýsek
mesh_terms: D000667:Ampicillin / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry*; D002506:Cephalexin / Q000032:analysis; D004563:Electrochemistry; D004566:Electrodes; D004573:Electrolytes; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D009821:Oils; D010783:Photometry; D013056:Spectrophotometry, Ultraviolet; D014867:Water
chemical_list: D000900:Anti-Bacterial Agents; D004573:Electrolytes; D007202:Indicators and Reagents; D009821:Oils; D014867:Water; D000667:Ampicillin; D002506:Cephalexin