[Expression of TGFb1 and its mRNA in liver tissues of patients with chronic viral hepatitis].
OBJECTIVE
To investigate the relationship between expression of transforming growth factor beta 1(TGF beta 1), TGF beta 1 mRNA in liver tissue of patients with chronic viral hepatitis and degree of liver fibrosis.


METHODS
TGF beta 1 protein was detected in 45 patients by immunohistochemistry and TGF beta 1 mRNA was detected in 21 of the 45 cases by in situ hybridization.


RESULTS
TGF beta 1 protein was 88.89%(40 of 45) positive in their liver tissues and was mainly distributed in interstitial cells, portal areas and the areas where fibrosis actively occurred, and the expression of TGF beta 1 protein was positively correlated with degree of liver fibrosis(r = 0.73, P < 0.05). TGF beta 1 mRNA was 80.95%(19 of 21)positive in their liver tissues. Positive stains were not only detected in sinusoidal cells and mononuclear cells, but also in some parenchymal cells.


CONCLUSIONS
The expression of TGF beta 1 correlated closely with liver fibrosis of viral hepatitis. Not only interstitial cells, but also parenchymal cells in liver might participate in the fibrogenisis by producing TGF beta 1.
Zhang
M
M
Hospital of PLA, Beijing.
Zhang
L
L
Long
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006521:Hepatitis, Chronic / Q000378:metabolism*; D006525:Hepatitis, Viral, Human / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis*; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta