LC method development for ibuprophen and validation in different pharmaceuticals.
An isocratic LC method for the simultaneous determination of ibuprophen (IBU) and its degradation product (4-isobutylacetophenone) has been developed and validated for different pharmaceuticals (sachets, tablets and gels). The chromatographic separation was achieved with phosphoric acid solution (pH 3.2)-acetonitrile (50:50, v/v) as mobile phase, a Hypersil C18 column and UV detection at 254 nm. In all cases, sample preparation was required before HPLC analysis.
Gasco-Lopez
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AI
Departamento de Química Analítica, Facultad de Química, Universidad Complutense, Madrid, Spain.
Izquierdo-Hornillos
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R
Jimenez
A
A
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D007052:Ibuprofen / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen