Native extraction of phosphotyrosine-containing proteins: requirement of tyrosine kinase inhibitors to obtain specific phosphorylation signals.
Johannsen
M
M
Department of Medicine I, Klinikum B. Franklin, Free University of Berlin, Hindenburgdamm 30, Berlin, 12200, Germany.
Rühl
M
M
Manski
D
D
Somasundaram
R
R
Riecken
E O
EO
Schuppan
D
D
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D003094:Collagen; D004791:Enzyme Inhibitors; D006801:Humans; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000032:analysis*; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D010750:Phosphoproteins; D019000:Phosphotyrosine; D003094:Collagen; D011505:Protein-Tyrosine Kinases; D019950:Mitogen-Activated Protein Kinase 1