Identification of iron-regulated genes of Helicobacter pylori by a modified fur titration assay (FURTA-Hp).
The Escherichia coli-based Fur titration assay (FURTA), although a powerful tool for identification of genes regulated by the ferric uptake regulator (Fur), was unsuccessful for the gastric pathogen Helicobacter pylori. The FURTA was modified by construction of an E. coli indicator strain producing H. pylori Fur only. The promoter regions of the ferric citrate receptor homolog fecA2 and the riboflavin synthesis gene ribBA were both positive in the modified FURTA, but negative in the original FURTA. Transcription of fecA2 and ribBA was demonstrated to be iron-repressed in H. pylori. This type of modification should allow FURTA analysis for bacteria with Fur binding sequences poorly recognized by E. coli Fur.
Fassbinder
F
F
University of Freiburg, Institute of Medical Microbiology and Hygiene, Department of Microbiology, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany.
van Vliet
A H
AH
Gimmel
V
V
Kusters
J G
JG
Kist
M
M
Bereswill
S
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D016480:Helicobacter pylori / Q000235:genetics* / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins / Q000378:metabolism; D018085:Regulon; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D015203:Reproducibility of Results; D012256:Riboflavin / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C028589:FecA protein, E coli; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron; D012256:Riboflavin