Iron overload and gene expression in HepG2 cells: analysis by differential display.
The aim of the present study was to evaluate the effect of iron overload on gene expression in HepG2 cells by differential display. Iron-treated cells showed a 50% decrease in apolipoprotein B100 (Apo B100) and a 2- and 3-fold increase in semaphorin cd100 and aldose reductase mRNA, respectively, with parallel variations in Apo B100 and aldose reductase proteins. These effects were time-dependent. Vitamin E prevented the increase in aldose reductase expression, but had no effect on Apo B100 and semaphorin cd100. Treatment with hydrogen peroxide and 4-hydroxy-2,3-nonenal increased only aldose reductase mRNA. These data suggest that iron can affect mRNA levels by lipid peroxidation-dependent and -independent pathways.
Barisani
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Cattedra di Gastroenterologia, Padiglione Granelli 3 P, IRCCS Ospedale Maggiore, Via F. Sforza 35, 20122, Milan, Italy. dbarisani@excite.com
Meneveri
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Ginelli
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Cassani
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Conte
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D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism; D015703:Antigens, CD; D001055:Apolipoproteins B / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D020869:Gene Expression Profiling; D006801:Humans; D007501:Iron / Q000378:metabolism*; D019190:Iron Overload / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000502:physiology; D008099:Liver / Q000166:cytology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D039961:Semaphorins; D014810:Vitamin E / Q000494:pharmacology
D015703:Antigens, CD; D001055:Apolipoproteins B; C093224:CD100 antigen; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D039961:Semaphorins; D014810:Vitamin E; D007501:Iron; D000449:Aldehyde Reductase