Growth and differentiation of the vascular smooth muscle and endothelial cells cultured on fluorine ion-implanted polystyrene.
The rat vascular (SMCs) and bovine endothelial cells (BECs) were cultured on conventional or fluorine ion-implanted polystyrene (5 x 10(12) and 5 x 10(14) fluorine ions/cm2). The cells grown on the implanted growth supports showed better adherence, higher volume and higher total protein content. The immunocytochemical analysis revealed that SMCs contained more of the cytoskeletal vimentin and the vascular SMC-specific alpha-actin as well as several cell adhesion-mediating molecules (vinculin, talin, alpha(v)-integrin and ICAM-1). In BECs, only the content of vimentin and talin increased, while expression of ICAM-1 was unchanged. The data suggest that cells on the ion implanted polymers could be more viable and that increased expression of some adhesion molecules mediating interactions with the host immune system is cell type-dependent.
Bacáková
L
L
Institute of Physiology, Academy of Sciences of Czech Republic, Prague. lucy@biomed.cas.cz
Mares
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Bottone
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MG
Pellicciari
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Lisá
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Svorcík
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D000199:Actins / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D005461:Fluorine; D007150:Immunohistochemistry; D039564:Integrin alphaV; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D011137:Polystyrenes; D051381:Rats; D016608:Talin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D000199:Actins; D015703:Antigens, CD; D039564:Integrin alphaV; D011137:Polystyrenes; D016608:Talin; D014746:Vimentin; D018799:Intercellular Adhesion Molecule-1; D005461:Fluorine