title: The retinoblastoma-interacting zinc-finger protein RIZ is a downstream effector of estrogen action.
abstract: Co-immunoprecipitation experiments in cell extract from cultured cells or target tissues indicated that estrogen receptor was complexed with the retinoblastoma binding protein RIZ in a ligand-dependent manner. Mapping of interaction sites indicated that in both proteins the same regions and motifs responsible for the interaction of transcriptional co-activator and nuclear receptors were involved. In cultured cells, estradiol induced a redistribution of RIZ protein within the nucleus and in the cytoplasm. A similar effect was produced in vivo, in prepuberal rat endometrium, by administration of a physiological dose of estradiol. Therefore, RIZ protein could be a specific effector of estrogen action downstream of the hormone-receptor interaction, presumably involved in proliferation control.
authors.affiliation: Istituto di Patologia generale ed Oncologia, Seconda Università degli studi di Napoli, Larghetto Sant'Aniello a Caponapoli 2, I-80138 Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Abbondanza
authors.forename: N
authors.initials: N
authors.lastname: Medici
authors.forename: V
authors.initials: V
authors.lastname: Nigro
authors.forename: V
authors.initials: V
authors.lastname: Rossi
authors.forename: L
authors.initials: L
authors.lastname: Gallo
authors.forename: G
authors.initials: G
authors.lastname: Piluso
authors.forename: A
authors.initials: A
authors.lastname: Belsito
authors.forename: A
authors.initials: A
authors.lastname: Roscigno
authors.forename: P
authors.initials: P
authors.lastname: Bontempo
authors.forename: A A
authors.initials: AA
authors.lastname: Puca
authors.forename: A M
authors.initials: AM
authors.lastname: Molinari
authors.forename: B
authors.initials: B
authors.lastname: Moncharmont
authors.forename: G A
authors.initials: GA
authors.lastname: Puca
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004268:DNA-Binding Proteins; D004967:Estrogens / Q000502:physiology*; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism; D014157:Transcription Factors; D016335:Zinc Fingers
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D004967:Estrogens; D009687:Nuclear Proteins; D011960:Receptors, Estrogen; D014157:Transcription Factors; D011495:Histone-Lysine N-Methyltransferase; C093563:PRDM2 protein, human