Chromosomal aberrations evaluated by CGH, FISH and GTG-banding in a case of AIDS-related Burkitt's lymphoma.
OBJECTIVE
We have previously reported on a complex chromosome rearrangement [der(17)] in a B-cell line, BRG A, established from an AIDS patient with Burkitt's lymphoma (BL). The aim of the present study was the definition of der(17) composition and the identification of complete or partial chromosome gains and losses in two cell clones (BRG A and BRG M) derived from this patient.


METHODS
We applied comparative genome hybridization (CGH) to detect the DNA misrepresentations in the genome of the two cell clones. Findings from CGH and banding analysis could then direct the choice of probes for chromosome painting experiments to elucidate der(17) composition.


RESULTS
CGH analysis identified gains of chromosomes 1q, 7q, 12q, 13q, 15q, 17p, 20p,q and losses of chromosomes 3p and 5q in BRG A and gain of chromosome 1q and loss in chromosome 6q in BRG M. Some of the detected alterations had already been described in lymphomas, while others appeared to be new. The combination of these techniques allowed a precise definition of der(17), composed by translocated regions from chromosomes 12 and 15.


CONCLUSIONS
We demonstrated CGH to be a powerful tool in the identification of recurrent chromosome aberrations in an AIDS-related BL and in ascertaining the origin of marker chromosomes. We were also able to identify a different pattern of aberrations and assess an independent sequence of events leading to the 1p gain in the two subclones.
Zunino
A
A
Mutagenesis Laboratory, National Cancer Institute (IST), largo Rosanna Benzi 10, 16132 Genova, Italy. zuninoa@hp380.ist.unige.it
Viaggi
S
S
Ottaggio
L
L
Fronza
G
G
Schenone
A
A
Roncella
S
S
Abbondandolo
A
A
D000782:Aneuploidy; D002051:Burkitt Lymphoma / Q000209:etiology / Q000235:genetics*; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002878:Chromosomes, Human, Pair 1; D002881:Chromosomes, Human, Pair 12; D002882:Chromosomes, Human, Pair 13; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002890:Chromosomes, Human, Pair 20; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D002897:Chromosomes, Human, Pair 7; D002999:Clone Cells; D003582:Cytogenetics / Q000379:methods*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D016483:Lymphoma, AIDS-Related / Q000235:genetics*; D014407:Tumor Cells, Cultured