title: A quantitative minimally invasive assay for the detection of metals in the stratum corneum.
abstract: A quantitative, minimally invasive tape-stripping assay for the detection of metals on and in skin that also has application to the detection of metallic elements on dry surfaces (where human contact could occur) has been developed. This development included construction, using commercial products, of an approximately 25 microm thick, low-metal content tape suitable both for tape-stripping and elemental analysis. Individual tapes were sequentially applied to the skin surface and then removed, taking with them a sample of the dead outer layer of the skin (stratum corneum). Analysis of such tape strip samples by particle induced X-ray emission (PIXE)--a well-characterized, sensitive, analytical technique based on X-ray spectrometry--identified and accurately quantified the metals in the sample. The assay had elemental sensitivities of approximately 1 ng/cm2 for many metals and analysis of elemental contents could be performed in as little as 5 min. The feasibility of the assay for measuring metals in the stratum corneum was demonstrated on the forearms of healthy human volunteers. Samples from approximately half the subjects were found to contain zirconium, possibly arising from the use of roll-on antiperspirants. The assay has potential as a tool: (1) for risk assessment, (2) to identify exposure levels following possible contact with a hazardous metal, and (3) to determine the effectiveness of cleanup or removal measures.
authors.affiliation: Department of Biopharmaceutical Sciences, School of Pharmacy, University of California San Francisco, 94143, USA. ccc@rebus.ucsf.edu
authors.forename: C
authors.initials: C
authors.lastname: Cullander
authors.forename: S
authors.initials: S
authors.lastname: Jeske
authors.forename: D
authors.initials: D
authors.lastname: Imbert
authors.forename: P G
authors.initials: PG
authors.lastname: Grant
authors.forename: G
authors.initials: G
authors.lastname: Bench
mesh_terms: D006801:Humans; D008670:Metals / Q000032:analysis*; D012016:Reference Values; D012867:Skin / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission
chemical_list: D008670:Metals