Glial and neuronal localization of ionotropic glutamate receptor subunit-immunoreactivities in the median eminence of female rats: GluR2/3 and GluR6/7 colocalize with vimentin, not with glial fibrillary acidic protein (GFAP).
Female rat median eminence was immunostained with anti-NR1, GluR1, GluR2/3, GluR6/7, or KA2. GluR2/3- and GluR6/7-immunoreactivities were detected in cells lining the basal portion of the third ventricle. To identify these cells as tanycytes, the median eminence was dual-immunostained with glutamate receptors and glial cytoskeletal marker proteins, such as vimentin or glial fibrillary acidic protein (GFAP). Both GluR2/3 and GluR6/7 were shown to colocalize with vimentin, not with GFAP. These results suggest the potential role for tanycytes in conducting glutamate signaling.
Kawakami
S
S
School of Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan. skawak@knaes.affrc.go.jp
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007150:Immunohistochemistry; D008473:Median Eminence / Q000378:metabolism* / Q000648:ultrastructure; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018091:Receptors, AMPA / Q000378:metabolism; D017470:Receptors, Glutamate / Q000378:metabolism*; D018092:Kainic Acid Receptors / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism*; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor
D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger; D018091:Receptors, AMPA; D017470:Receptors, Glutamate; D018092:Kainic Acid Receptors; D014746:Vimentin; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor; C104723:glutamate receptor ionotropic, AMPA 3; C104722:glutamate receptor ionotropic, AMPA 2