title: Fundoplication: a model for immunologic aspects of laparoscopic and conventional surgery.
abstract: OBJECTIVE
  Immunologic investigations of laparoscopic and conventional procedures have recently been performed during cholecystectomy or colon resection, but the results might have been influenced by the amount of dissection or the presence of malignant tumor. Because fundoplication is characterized by moderate dissection and no resection, we supposed it to be an appropriate procedure for comparing immunologic changes during laparoscopic and conventional surgery.
  
  
  METHODS
  Immunologic analysis (interleukin [IL]-6, IL-10, leukocytes, HLA-DR monocytes) was carried out on the peripheral blood of 34 patients who underwent elective Nissen fundoplication by the laparoscopic (LAP; N = 26) or conventional (OPEN; N = 8) technique for gastroesophageal reflux disease. Blood samples were obtained before and 1 and 4 hours after the beginning of the operation and on days 1, 2, 4, 7 after the procedure.
  
  
  RESULTS
  A very fast and significant (P < 0.01) increase of the proinflammatory cytokines (IL-6, IL-10) and leukocytes and a decrease of cell-mediated functions (HLR-DR monocytes) were detected. Most of the analyzed measures had returned to preoperative values by 2 days after the procedure. All of the changes were similar in the two groups with the exception of IL-6. Throughout the post-operative study period, IL-6 concentrations were higher in the OPEN group, being significant 4 hours, 1 day, 2 days, and 4 days after the operation.
  
  
  CONCLUSIONS
  The investigation measures do not give evidence that laparoscopic fundoplication is superior to conventional fundoplication in its immunologic effects.
authors.affiliation: Department of Surgery, Charité, Humboldt University of Berlin, Germany. zieren@rz.charite.hu-berlin.de
authors.forename: J
authors.initials: J
authors.lastname: Zieren
authors.forename: C A
authors.initials: CA
authors.lastname: Jacobi
authors.forename: F A
authors.initials: FA
authors.lastname: Wenger
authors.forename: H D
authors.initials: HD
authors.lastname: Volk
authors.forename: J M
authors.initials: JM
authors.lastname: Müller
mesh_terms: D000328:Adult; D016207:Cytokines / Q000097:blood*; D005260:Female; D018662:Fundoplication / Q000379:methods*; D005764:Gastroesophageal Reflux / Q000601:surgery*; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D007111:Immunity, Cellular; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D010535:Laparoscopy; D008297:Male; D011446:Prospective Studies
chemical_list: D016207:Cytokines; D006684:HLA-DR Antigens; D015850:Interleukin-6; D016753:Interleukin-10