No evidence of correlation between mutation at codon 531 of src and the risk of colon cancer in Chinese.
The protein tyrosine kinase activity of c-src proto-oncogene product, pp60(c-src), is elevated in a number of human cancers, including colon cancer. Phosphorylation of human pp60(c-src) carboxy-terminal tyrosine 530 suppresses its kinase activity. A recent report suggested that the risk of colon cancer is higher for those who carry a C-->T transition mutation on codon 531 (Gln-531-->Amber-531) of src gene. This mutation caused a prematured translation termination and up-regulated the kinase activity. To examine whether this mutation could be a risk factor for colon carcinoma in the Chinese population, we used the same PCR-based assay to analyze src genotypes of 131 colon cancers and other various types of carcinoma. No mutation was detected in all specimens that were screened in this study. Thus, mutation at Gln-531 of src gene does not seem to be involved in the development of colon cancer in Chinese ethnicity.
Wang
N M
NM
Division of Molecular Medicine, Department of Medical Research, China Medical College Hospital, Taichung, Taiwan.
Yeh
K T
KT
Tsai
C H
CH
Chen
S J
SJ
Chang
J G
JG
D002681:China; D003062:Codon; D003110:Colonic Neoplasms / Q000208:ethnology / Q000209:etiology / Q000235:genetics*; D016391:Genes, src; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas
D003062:Codon; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas