Detailed monitoring of hematopoietic chimerism in a child treated by adoptive immunotherapy for high risk of relapse after BMT for acute myeloid leukemia.
We report a case of a 13-year-old boy who was transplanted for relapse of acute myeloid leukemia (AML). A detailed study of hematopoietic chimerism was performed using polymerase chain reaction (PCR) of variable number of tandem repeats (VNTR) at very short time intervals. We used discontinuation of post-transplant immunosuppression and donor lymphocyte infusions (DLI) in order to prevent leukemia relapse that was indicated by a progressive increase in autologous hematopoiesis. Despite the fact that the boy relapsed 10 months after BMT, we could see a significant influence of adoptive immunotherapy on the mixed chimerism status during the post-transplant period.
Formánková
R
R
Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Honzátková
L
L
Sieglová
Z
Z
Starý
J
J
Sedlácek
P
P
Brdicka
R
R
D000208:Acute Disease; D000293:Adolescent; D016026:Bone Marrow Transplantation; D003131:Combined Modality Therapy; D006410:Hematopoiesis / Q000235:genetics / Q000276:immunology; D006801:Humans; D016219:Immunotherapy, Adoptive; D007951:Leukemia, Myeloid / Q000276:immunology / Q000628:therapy*; D008297:Male; D055502:Secondary Prevention; D018183:Transplantation Chimera; D014184:Transplantation, Homologous