Effect of glutathione-modulating compounds on platinum compounds-induced cytotoxicity in human glioma cell lines.
The relation between the effect of glutathione(GSH)-modulating compounds and platinum compounds (Cisplatin, Nedaplatin)-induced cytotoxicity was investigated. Pretreatment of human glioblastoma (T98G, U87MG) and glioma (KG1C) cell lines with L-buthionine-[S,R]-sulfoximine, which decrease the intracellular GSH concentration, remarkably increased their sensitivity against platinum compounds, whereas pretreatment with N-acetyl-L-cysteine, which increase the intracellular GSH concentration, only marginally protected the cells from the cytotoxic effect of platinum compounds. The results suggest that platinum compounds-induced cytotoxicity can be modified by GSH-modulating compounds in glioblastoma and glioma cell lines.
Iida
M
M
Department of Neurosurgery, Tokyo Metropolitan Ebara Hospital, Japan. masaiida-e@ebara-hp.ota.tokyo.jp
Doi
H
H
Asamoto
S
S
Sugiyama
H
H
Sakagami
H
H
Kuribayashi
N
N
Takeda
M
M
Okamura
Y
Y
Matsumoto
K
K
D000111:Acetylcysteine / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology; D001932:Brain Neoplasms / Q000473:pathology*; D019328:Buthionine Sulfoximine / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D004357:Drug Synergism; D005910:Glioma / Q000473:pathology*; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D019328:Buthionine Sulfoximine; C053989:nedaplatin; D005978:Glutathione; D002945:Cisplatin; D000111:Acetylcysteine