title: Full and fractionated experimental designs for robustness testing in the high-performance liquid chromatographic analysis of codeine phosphate, pseudoephedrine hydrochloride and chlorpheniramine maleate in a pharmaceutical preparation.
abstract: This paper describes the testing of a saturated factorial design using a full factorial design. Saturated factorial designs are often used to test the robustness of high-performance liquid chromatography (HPLC) methods, however they are based on several assumptions. A full factorial design relies on fewer assumptions and hence could be used to evaluate the effectiveness of the saturated design. Both designs were used to test a gradient HPLC method for the assay of codeine phosphate, pseudoephedrine hydrochloride and chlorpheniramine maleate. Six HPLC conditions, including wavelength, mobile phase pH and ion pairing reagent concentration were tested using the saturated design. Three of these factors were selected for full evaluation using a full factorial design. The results showed that the main effects calculated by each design were comparable. However, the saturated design showed higher standard errors, probably due to the effects of changing several more factors. One interaction effect was indicated as a confounding effect by the saturated design and this was confirmed by the calculation of the same interaction effect using the full design. Overall the method was shown to be robust under the variety of HPLC conditions tested.
authors.affiliation: Department of Chemistry, Materials and Forensic Science, University of Technology, Sydney, NSW, Australia.
authors.forename: R
authors.initials: R
authors.lastname: Ragonese
authors.forename: M
authors.initials: M
authors.lastname: Mulholland
authors.forename: J
authors.initials: J
authors.lastname: Kalman
mesh_terms: D002744:Chlorpheniramine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000032:analysis*; D004809:Ephedrine / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*
chemical_list: D004364:Pharmaceutical Preparations; D002744:Chlorpheniramine; D004809:Ephedrine; D003061:Codeine