title: The structures of the H(C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding.
abstract: The entry of tetanus neurotoxin into neuronal cells proceeds through the initial binding of the toxin to gangliosides on the cell surface. The carboxyl-terminal fragment of the heavy chain of tetanus neurotoxin contains the ganglioside-binding site, which has not yet been fully characterized. The crystal structures of native H(C) and of H(C) soaked with carbohydrates reveal a number of binding sites and provide insight into the possible mode of ganglioside binding.
authors.affiliation: Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Emsley
authors.forename: C
authors.initials: C
authors.lastname: Fotinou
authors.forename: I
authors.initials: I
authors.lastname: Black
authors.forename: N F
authors.initials: NF
authors.lastname: Fairweather
authors.forename: I G
authors.initials: IG
authors.lastname: Charles
authors.forename: C
authors.initials: C
authors.lastname: Watts
authors.forename: E
authors.initials: E
authors.lastname: Hewitt
authors.forename: N W
authors.initials: NW
authors.lastname: Isaacs
mesh_terms: D000116:Acetylgalactosamine / Q000737:chemistry; D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry*; D018360:Crystallography, X-Ray; D005690:Galactose / Q000737:chemistry; D005732:Gangliosides / Q000378:metabolism*; D007785:Lactose / Q000737:chemistry; D008958:Models, Molecular; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry*; D011485:Protein Binding; D013744:Tetanus Toxin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D005732:Gangliosides; D010446:Peptide Fragments; D013744:Tetanus Toxin; D019158:N-Acetylneuraminic Acid; D007785:Lactose; D000116:Acetylgalactosamine; D005690:Galactose