Protein phosphatase 1beta is required for the maintenance of muscle attachments.
Type 1 serine/threonine protein phosphatases (PP1) are important regulators of many cellular and developmental processes, including glycogen metabolism, muscle contraction, and the cell cycle [1] [2] [3] [4] [5]. Drosophila and humans both have multiple genes encoding PP1 isoforms [3] [6] [7]; each has one beta and several alpha isoform genes (alpha(1), alpha(2), alpha(3) in flies, alpha and gamma in humans; mammalian PP1beta is also known as PP1delta). The alpha/beta subtype differences are highly conserved between flies and mammals [6]. Though all these proteins are >85% identical to each other and have indistinguishable activities in vitro, we show here that the Drosophila beta isoform has a distinct biological role. We show that PP1beta9C corresponds to flapwing (flw), previously identified mutants of which are viable but flightless because of defects in indirect flight muscles (IFMs) [8]. We have isolated a new, semi-lethal flw allele that shows a range of defects, especially in muscles, which break away from their attachment sites and degenerate.
Raghavan
S
S
National Centre for Biological Sciences, UAS/GKVK Campus, Bangalore, 560065, India.
Williams
I
I
Aslam
H
H
Thomas
D
D
Szöor
B
B
Morgan
G
G
Gross
S
S
Turner
J
J
Fernandes
J
J
VijayRaghavan
K
K
Alphey
L
L
D000483:Alleles; D000818:Animals; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D008957:Models, Genetic; D005810:Multigene Family; D009132:Muscles / Q000737:chemistry / Q000502:physiology*; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000502:physiology*; D020033:Protein Isoforms; D054645:Protein Phosphatase 1; D013696:Temperature; D014921:Wings, Animal / Q000502:physiology
D020033:Protein Isoforms; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1