The GABA-withdrawal syndrome: a model of local status epilepticus.
The GABA-withdrawal syndrome (GWS) is a model of local status epilepticus following the interruption of a chronic GABA infusion into the rat somatomotor cortex. GWS is characterized by focal epileptic electroencephalographic discharges and associated contralateral myoclonus. In neocortical slices obtained from GWS rats, most neurons recorded in the GABA-infused area are pyramidal neurons presenting bursting properties. The bursts are induced by white-matter stimulation and/or intracellular depolarizing current injection and correlate with a decrease of cellular sensitivity to GABA, caused by its prolonged infusion. This effect is related to a calcium influx that may reduce the GABAA receptor-mediated inward current and is responsible for the bursting properties. Here we present evidence for the involvement of calcium- and NMDA-induced currents in burst genesis. We also report modulatory effects of noradrenaline appearing as changes on firing patterns of bursting and nonbursting cells. Complementary histochemical data reveal the existence of a local noradrenergic hyperinnervation and an ectopic expression of tyrosine hydroxylase mRNAs in the epileptic zone.
Silva-Barrat
C
C
Laboratoire de Génétique de la Neurotransmission et des Processus Neurodégénératifs, UMR 9923, CNRS, Paris, France. silvabar@ccr.jussieu.fr
Champagnat
J
J
Menini
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C
D000818:Animals; D002118:Calcium / Q000502:physiology; D004195:Disease Models, Animal; D004351:Drug Resistance; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D009474:Neurons / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000502:physiology; D009638:Norepinephrine / Q000502:physiology; D013226:Status Epilepticus / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D013375:Substance Withdrawal Syndrome; D005680:gamma-Aminobutyric Acid / Q000009:adverse effects* / Q000494:pharmacology
D018377:Neurotransmitter Agents; D005680:gamma-Aminobutyric Acid; D002118:Calcium; D009638:Norepinephrine