title: Purine metabolites and malondialdehyde in platelets of diabetic patients.
abstract: The concentration of some of the purine nucleotides and their metabolites together with that of malondialdehyde (MDA) have been measured in resting and stimulated platelets of type 1 and type 2 diabetic patients. While control platelets show a net decrease of guanosine triphosphate (GTP) (3.1 vs. 2.3 nmol per 10(9) platelets) and guanosine diphosphate (GDP) (3.0 vs. 2.0 nmol per 10(9) platelets) and a significant increase of adenosine (0.04 vs. 0.55 nmol per 10(9) platelets) with platelet stimulation, platelets of type 1 and type 2 diabetic patients have a lesser change of these metabolites (GTP, 2.6 vs. 2.4; GDP, 2.3 vs. 2.4; adenosine, 0.04 vs. 0.30 (P < 0.05 vs. control) nmol per 10(9) platelets in type 1 diabetics; GTP, 2.4 vs. 2.7; GDP, 2.4 vs. 2.1; adenosine, 0.08 vs. 0.32 (P < 0.05 vs. control) nmol per 10(9) platelets in type 2 diabetics). These results indicate that the change (stimulated minus resting) of GTP, GDP and adenosine in diabetic platelets is significantly different from that of controls (P < 0.001). Moreover, the amount of MDA produced during platelet activation seems to be lower than controls only in type 2 diabetes (1.81 vs. 2.86 nmol per 10(9) platelets, P < 0.05). These results seem to indicate that a difference in the pattern of platelet nucleotides could be an important feature even in well-controlled diabetes, while MDA is probably modified only in association with the late vascular complications of diabetes.
authors.affiliation: Lab. Di Chimica Clinica-Policlinico A. Gemelli, Istituto di Chimica e Chimica Clinica and Centro CNR per lo Studio della Chimica dei Recettori e delle Sostanze Biologicamente Attive, Rome, Italy. b.zappacosta@uniserv.ccr.rm.cnr.it
authors.forename: B
authors.initials: B
authors.lastname: Zappacosta
authors.forename: P
authors.initials: P
authors.lastname: De Sole
authors.forename: S
authors.initials: S
authors.lastname: Persichilli
authors.forename: D
authors.initials: D
authors.lastname: Pitocco
authors.forename: G
authors.initials: G
authors.lastname: Marra
authors.forename: G
authors.initials: G
authors.lastname: Ghirlanda
authors.forename: B
authors.initials: B
authors.lastname: Giardina
mesh_terms: D000241:Adenosine / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000293:Adolescent; D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006150:Guanine Nucleotides / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D011687:Purines / Q000378:metabolism*; D012016:Reference Values
chemical_list: D006150:Guanine Nucleotides; D011687:Purines; D000249:Adenosine Monophosphate; D008315:Malondialdehyde; D000241:Adenosine