Tumor-associated glycoprotein 72 (TAG-72) expression in salivary gland neoplasia: an immunohistochemical study using the monoclonal antibody (MAb) CC49.
OBJECTIVE
The purpose of this study was to investigate immunohistochemically the expression of tumor-associated glycoprotein 72 (TAG-72) using the monoclonal antibody (MAb) CC49 in salivary gland neoplasia and normal salivary glands in an attempt to determine the potential usefulness of MAb CC49 in diagnostic and therapeutic applications.


METHODS
Eighty-six specimens (21 benign tumors, 41 malignant, and 24 normal salivary glands), fixed in 10% formalin and embedded in paraffin, were retrieved from the files of the Department of Oral Medicine and Oral Pathology at the Dental School of Aristotle University, Thessaloniki, Greece, and were retrospectively studied with hematoxylin and eosin and with the streptavidin-biotin-complex method using the MAb CC49.


RESULTS
Strong immunoreactivity for TAG-72 was observed in salivary duct carcinoma, adenocarcinoma, papillary cystadenocarcinoma, low-grade mucoepidermoid carcinoma, normal submandibular, sublingual, and minor salivary glands. Weak or no immunoreactivity was found in adenoid cystic carcinoma, basal cell adenocarcinoma, polymorphous low-grade adenocarcinoma, and normal parotid gland.


CONCLUSIONS
Our results suggest the potential use of MAb CC49 in the differential diagnosis of some salivary gland neoplasms in which their histopathologic features overlap, and in the radiation immunolocalization and immunotherapy of malignant tumors that are localized in the parotid gland.
Epivatianos
A
A
Department of Oral Medicine and Oral Pathology, Dental School, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece.
Poulopoulos
A
A
Kayavis
I
I
Papanayotou
P
P
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002277:Carcinoma / Q000235:genetics / Q000473:pathology; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000473:pathology; D018277:Carcinoma, Mucoepidermoid / Q000235:genetics / Q000473:pathology; D004396:Coloring Agents; D018283:Cystadenocarcinoma, Papillary / Q000235:genetics / Q000473:pathology; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics; D006416:Hematoxylin; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D010306:Parotid Gland / Q000166:cytology / Q000378:metabolism; D012189:Retrospective Studies; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000473:pathology*; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism; D012470:Salivary Glands, Minor / Q000166:cytology / Q000378:metabolism; D013361:Sublingual Gland / Q000166:cytology / Q000378:metabolism; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C091202:B72.3 antibody; D014408:Biomarkers, Tumor; D004396:Coloring Agents; D005456:Fluorescent Dyes; D006023:Glycoproteins; C047052:tumor-associated antigen 72; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin