CYP11B2 gene polymorphisms in idiopathic hyperaldosteronism.
Primary aldosteronism is characterized by autonomous production of aldosterone and arterial hypertension, and it occurs in 2 principal forms: aldosterone-producing adenoma (APA) and idiopathic hyperaldosteronism (IHA). APA can be cured through removal of the adenoma, whereas IHA leads to hypertension that must be treated with medication. The origin of the autonomous aldosterone production in IHA is poorly understood, but genetic factors may contribute to its cause. To test the hypothesis that variants of the aldosterone synthase gene may contribute to susceptibility to IHA, we compared genotypes at 3 polymorphic sites in the CYP11B2 gene in patients with IHA (n=90) with those found in patients with APA (n=38), in patients with essential hypertension (n=72), and in normotensive individuals (n=102). We observed significant linkage disequilibrium among the 3 polymorphisms with 2 frequent haplotypes in all groups studied. One haplotype (C2R) was found to be increased in frequency in the IHA group (47%) compared with the other groups, which had a similar haplotype frequency (36%). The 3 polymorphisms studied have been implicated in either essential hypertension or excess aldosterone production in previous studies. Because of the strong linkage disequilibrium, the observed results could be due to the action of any 1 of the 3 alleles or to another allele in linkage disequilibrium with them. Our results suggest that variations in the CYP11B2 gene may contribute to dysregulation of aldosterone synthesis and lead to susceptibility to IHA.
Mulatero
P
P
Department of Medicine and Experimental Oncology, Hypertension Unit, University of Torino, Torino, Italy. mulatero@tin.it
Schiavone
D
D
Fallo
F
F
Rabbia
F
F
Pilon
C
C
Chiandussi
L
L
Pascoe
L
L
Veglio
F
F
D000483:Alleles; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D006239:Haplotypes; D006801:Humans; D006929:Hyperaldosteronism / Q000209:etiology / Q000235:genetics*; D006977:Hypertension, Renal / Q000209:etiology / Q000235:genetics*; D007438:Introns / Q000235:genetics; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers; D019405:Cytochrome P-450 CYP11B2