Purification and characterization of UDP-glucuronate: baicalein 7-O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures.
UDP-glucuronate: baicalein 7-O-glucuronosyltransferase (UBGAT) catalyzes the transfer of glucuronic acid from UDP-glucuronic acid to the 7-OH of baicalein. UBGAT was purified from cultured cells of Scutellaria baicalensis Georgi (Lamiaceae). It was purified 95-fold using various chromatography and chromatofocusing procedures to apparent homogeneity. The Mr was estimated to be 110 kDa by gel filtration chromatography with a 52 kDa subunit by SDS-PAGE. The isoelectric point was pH 4.8. UBGAT was specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such its baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe).
Nagashima
S
S
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Hirotani
M
M
Yoshikawa
T
T
D002850:Chromatography, Gel; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D044950:Flavanones; D005419:Flavonoids / Q000737:chemistry; D014453:Glucuronosyltransferase / Q000737:chemistry / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D013379:Substrate Specificity; D014535:Uridine Diphosphate Glucuronic Acid / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D010940:Plant Proteins; D014535:Uridine Diphosphate Glucuronic Acid; C006680:baicalein; C411122:UDP-glucuronate baicalein 7-O-glucuronosyltransferase; D014453:Glucuronosyltransferase