Deferiprone (L1) induced conformation change of hemoglobin: A fluorescence and CD spectroscopic study.
The interaction of deferiprone (1,2-dimethyl-3-hydroxy-pyrid-4-one) L1, the first clinically available oral iron chelator, with the tetrameric allosteric protein hemoglobin from human red blood cells has been investigated spectrofluorometrically and by circular dichroism spectroscopy. The interaction is hydrogenbond like electrostatic in nature, the binding constant being 4.54 x 10(3) M(-1) in 0.15 M NaCl. Circular dichroism studies indicate a conformational change of hemoglobin in presence of deferiprone, helicity of hemoglobin being reduced in presence of increasing concentration of the drug L1.
Chakraborty
D
D
Department of Biochemistry, University college of Science, University of Calcutta, India.
Bhattacharyya
M
M
D002942:Circular Dichroism; D000077543:Deferiprone; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D011728:Pyridones / Q000378:metabolism / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence / Q000379:methods
D006454:Hemoglobins; D007502:Iron Chelating Agents; D011728:Pyridones; D000077543:Deferiprone; D010100:Oxygen