title: Start sites of bidirectional DNA synthesis at the human lamin B2 origin.
abstract: The initiation sites of bidirectional synthesis at the DNA replication origin located at the 3' end of the human lamin B2 gene were investigated. RNA-primed nascent DNA molecules were subjected to second-strand synthesis with appropriate primers, amplified by ligation-mediated polymerase chain reaction, and size fractionated. Evidence for precise start sites was obtained. Exploration of close to 1 kilobase, coupled to inhibition of Okazaki fragment synthesis, demonstrates that the leading strands initiate at precise nucleotides on either helix, overlapping by three base pairs, within the area bound to a protein complex possibly analogous to the prereplicative complex of yeast.
authors.affiliation: Molecular Biology and Molecular Medicine Units, International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012 Trieste, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Abdurashidova
authors.forename: M
authors.initials: M
authors.lastname: Deganuto
authors.forename: R
authors.initials: R
authors.lastname: Klima
authors.forename: S
authors.initials: S
authors.lastname: Riva
authors.forename: G
authors.initials: G
authors.lastname: Biamonti
authors.forename: M
authors.initials: M
authors.lastname: Giacca
authors.forename: A
authors.initials: A
authors.lastname: Falaschi
mesh_terms: D020856:AT Rich Sequence; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis; D017931:DNA Primers / Q000378:metabolism; D004261:DNA Replication; D004640:Emetine / Q000494:pharmacology; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D034921:Lamin Type B; D034882:Lamins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D011485:Protein Binding; D018741:Replication Origin; D016196:S Phase
chemical_list: D017931:DNA Primers; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; C022262:Okazaki fragments; D004247:DNA; D004640:Emetine