Species-dependent pharmacological properties of the melanocortin-5 receptor.
The genes encoding the melanocortin-3 receptor and melanocortin-5 receptor have been cloned from rhesus monkey. Heterologous expression in CHO cells indicated species dependent in vitro pharmacological properties for the human and rhesus melanocortin-5 receptors. Several peptides including NDP-alpha-MSH, alpha-MSH, MT-II and ACTH1-24 are more potent at the rhesus melanocortin-5 receptor than the human melanocortin-5 receptor by more than 10-fold. In contrast, we found no species difference in pharmacological properties between the human and rhesus melanocortin-3 receptors. Such a species-dependent pharmacological difference for melanocortin-5 receptor appears to be an exception compared to other G protein-coupled receptors from human and rhesus monkey.
Huang
R R
RR
Department of Obesity Research, Merck Research Laboratories, Rahway, NJ 07065, USA.
Singh
G
G
Van der Ploeg
L H
LH
Fong
T M
TM
D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D044104:Receptor, Melanocortin, Type 3; D018043:Receptors, Corticotropin / Q000737:chemistry / Q000502:physiology*; D044101:Receptors, Melanocortin; D013045:Species Specificity
D044104:Receptor, Melanocortin, Type 3; D018043:Receptors, Corticotropin; D044101:Receptors, Melanocortin; C086971:melanocortin 5 receptor