An inductively coupled plasma method for determination of cyclophosphamide loaded to polymeric systems.
A new method for the determination of cyclophosphamide content of polyalkylcyanoacrylate nanoparticles was developed. The analyses were carried out by inductively coupled plasma atomic emission spectrometry (ICP-AES) by measuring the phosphorus content in the drug. The results obtained by this non-selective technique were compared with those given by high performance liquid chromatography (HPLC) a selective procedure that permits the detection of the cyclophosphamide molecule, and its degradation products. Sensitivity and reproducibility of both procedures were also determined. The ICP-AES method was demonstrated to be valid for sensitivity, precision, accuracy and specificity. In spite of ICP method is not a suitable procedure to analyze the degradation products of cyclophosphamide, the sensitivity of ICP is higher than chromatographic technique. Nevertheless, both procedures are appropriate for the determination of cyclophosphamide-loaded nanoparticles.
Salgueiro
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A
Physicochemistry Department, Faculty of Pharmacy, University of Barcelona, Spain.
Egea
M A
MA
Valls
R
R
Espina
M
M
García
M L
ML
D018906:Antineoplastic Agents, Alkylating / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003520:Cyclophosphamide / Q000032:analysis*; D004355:Drug Stability; D011108:Polymers / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D013052:Spectrometry, X-Ray Emission / Q000379:methods
D018906:Antineoplastic Agents, Alkylating; D011108:Polymers; D003520:Cyclophosphamide