Investigation of expression of HOX 2C and HOX 4B homeobox genes in human colorectal cancer by using an RT-PCR method.
Investigation of expression of both HOX 2C and HOX 4B homeobox genes in the same patient with colorectal cancer was proposed by using an RT-PCR method. In order to conduct this investigation, PCR products of 445 bp of HOX 2C and 301 bp of HOX 4B were amplified in both tumor and normal samples of ten patients. Expressions of HOX 2C gene were observed in both tumor and normal samples of four patients and in only a tumor sample of one patient, while the expression was not observed in both tumor and normal samples of five patients. Expressions of HOX 4B gene were not observed in both tumor and normal samples of ten patients. In the present study, it was found that individuality seems to be important. The results of these two genes, observed in patients with colorectal cancer, should be taken into consideration for further researches.
Polat
M F
MF
Biotechnology Application and Research Center, Atatürk University, Erzurum, Turkey.
Nalbantoglu
B
B
D015179:Colorectal Neoplasms / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D015972:Gene Expression Regulation, Neoplastic; D005801:Genes, Homeobox; D006801:Humans; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012334:RNA, Neoplasm