title: Cellular expression of the neurokinin 1 receptor in the human antrum.
abstract: The localization of the neurokinin 1 receptor in rat and guinea pig gastrointestinal tract has been extensively studied but not in human tissues. The present study used antibodies to characterize the cellular expression of neurokinin 1 receptors in human antrum. Cryostat sections (40-80 microm) were immunostained for the neurokinin 1 receptor double labeled with substance P, von Willebrand's factor, c-kit, fibronectin, S-100, serotonin, gastrin and somatostatin. Neurokinin 1 receptor-immunoreactivity was observed on neurons within the myenteric and submucosal plexuses surrounded by substance P-immunoreactive fibers and on von Willebrand's factor-immunoreactive endothelial cells lining blood vessels throughout the antral wall. c-Kit-immunoreactive interstitial cells of Cajal and gastrin cells were co-stained by the monoclonal neurokinin 1 receptor antibody. Finally, there was no evidence for the presence of the neurokinin 1 receptor on fibroblasts, Schwann, somatostatin, serotonin or smooth muscle cells. This study clearly demonstrates an expanded cellular expression of the neurokinin 1 receptor in the human antrum.
authors.affiliation: Department of Physiology, 2146 Health Sciences Mall, University of British Columbia, Vancouver, Canada.
authors.forename: V C
authors.initials: VC
authors.lastname: Smith
authors.forename: M A
authors.initials: MA
authors.lastname: Sagot
authors.forename: H
authors.initials: H
authors.lastname: Wong
authors.forename: A M
authors.initials: AM
authors.lastname: Buchan
mesh_terms: D000293:Adolescent; D000328:Adult; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D011706:Pyloric Antrum / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D013368:Submucous Plexus / Q000166:cytology / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D005755:Gastrins; D018040:Receptors, Neurokinin-1; D014841:von Willebrand Factor; D013373:Substance P; D019009:Proto-Oncogene Proteins c-kit