Screening for Drosophila proteins with distinct expression patterns during development by use of monoclonal antibodies.
Kc 167 is a cell line established from Drosophila embryonic hemocytes and has been shown to express many extracellular matrix (ECM) and other proteins important during development. We have screened monoclonal antibodies (mAbs) raised against heparin affinity purified proteins from conditioned medium of Kc 167 cells to identify novel proteins with important roles for development. One mAb recognized a protein expressed with temporary and tissue specific patterns during Drosophila embryogenesis and larval development. This approach is an alternative to screening of Expression Sequence Tag (EST) clones by in situ hybridization to initiate reverse genetics. In addition, a number of mAbs recognizing ECM proteins were also identified. These mAbs will be useful for biochemical and cell biological analyses of Drosophila ECM proteins.
Kumagai
T
T
Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Yokoyama
H
H
Goto
A
A
Hirose
J
J
Kadowaki
T
T
Narita
H
H
Kitagawa
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002460:Cell Line; D002846:Chromatography, Affinity; D004330:Drosophila / Q000196:embryology* / Q000254:growth & development; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000276:immunology; D017403:In Situ Hybridization; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D016324:Sequence Tagged Sites
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D011506:Proteins