The reduction of the Rieske iron-sulfur cluster in naphthalene dioxygenase by X-rays.
Naphthalene 1,2 dioxygenase (NDO) displays characteristic UV-Vis spectra depending on the oxidation state of the Rieske center. Investigations on crystals of NDO grown for X-ray diffraction experiments showed spectra characteristic of the oxidized form. Crystals reduced in an anaerobic glovebox using sodium-dithionite showed a characteristic reduced spectrum. Spectra of crystals (cooled to 100 K) after being exposed to X-rays for data collection showed spectra corresponding to a reduced Rieske iron center, demonstrating the ability of X-rays to change the oxidation state of the Rieske iron-sulfur cluster in NDO.
Karlsson
A
A
Department of Molecular Biology, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala.
Parales
J V
JV
Parales
R E
RE
Gibson
D T
DT
Eklund
H
H
Ramaswamy
S
S
D003460:Crystallization; D049308:Dioxygenases; D004926:Escherichia coli / Q000201:enzymology; D007501:Iron / Q000378:metabolism / Q000528:radiation effects*; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D015591:Microspectrophotometry; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D013455:Sulfur / Q000378:metabolism / Q000528:radiation effects*; D013696:Temperature; D013997:Time Factors; D014965:X-Rays
D007506:Iron-Sulfur Proteins; D009097:Multienzyme Complexes; D013455:Sulfur; D007501:Iron; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase