Lipid peroxidation in workers exposed to hexavalent chromium.
The aim of this study was to investigate whether exposure to hexavalent chromium induces lipid peroxidation in human. This study involved 25 chrome-plating factory workers and a reference group of 28 control subjects. The whole-blood and urinary chromium concentrations were determined by graphite furnace atomic absorption spectrophotometry. Malondialdehyde (MDA), the product of lipid peroxidation, was determined by high-performance liquid chromatography, and the activities of protective enzymes were measured by ultraviolet-visible spectrophotometry. In the chrome-plating workers, the mean concentrations of chromium in blood and urine were 5.98 microg/L and 5.25 microg/g creatinine, respectively; the mean concentrations of MDA in blood and urine were 1.7 micromol/L and 2.24 micromol/g creatinine. The concentrations of both chromium and MDA in blood and urine were significantly higher in the chromium-exposed workers. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) were not markedly different between control and exposed workers. Data suggest that MDA may be used as a biomarker for occupational chromium exposure. Antioxidant enzymic activities are not a suitable marker for chromium exposure.
Huang
Y L
YL
Graduate Institute of Medicine and School of Technology for Medical Sciences, Kaohsiung Medical College, Taiwan, Republic of China.
Chen
C Y
CY
Sheu
J Y
JY
Chuang
I C
IC
Pan
J H
JH
Lin
T H
TH
D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002274:Carcinogens, Environmental / Q000009:adverse effects*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D002857:Chromium / Q000009:adverse effects* / Q000097:blood / Q000652:urine; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000652:urine; D008669:Metallurgy; D009784:Occupational Diseases / Q000097:blood / Q000139:chemically induced* / Q000652:urine; D016273:Occupational Exposure / Q000009:adverse effects*
D015415:Biomarkers; D002274:Carcinogens, Environmental; D002857:Chromium; C074702:chromium hexavalent ion; D008315:Malondialdehyde