Purification, crystallization and x-ray analysis of crystals of pectate lyase A from Exwinia chrysanthemi.
Pectate lyase A is secreted by Erwinia chrysanthemi and is a virulence factor for soft rot diseases in plants. Crystals of pectate lyase A were obtained by vapor-diffusion techniques in the presence of polyethylene glycol. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 48.96, b = 148.86, c = 78.61 A, beta = 97.32 degrees. The crystals contain two protein molecules of 38 kDa per asymmetric unit and diffract to 2.4 A using Cu Kalpha radiation.
Doan
C N
CN
School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110--2499, USA.
Caughron
M K
MK
Myers
J C
JC
Breakfield
N W
NW
Oliver
R L
RL
Yoder
M D
MD
D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D016974:Dickeya chrysanthemi / Q000201:enzymology*; D008958:Models, Molecular; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation
D001426:Bacterial Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase