title: Peripheral blood progenitor cell mobilization in healthy donors receiving recombinant human granulocyte colony-stimulating factor.
abstract: OBJECTIVE
  We analyzed the incidence of primitive (LTC-IC) and committed (CFU-mix, BFU-E, CFU-GM) hematopoietic progenitors detected under steady-state conditions and upon progenitor cell mobilization in a cohort of healthy donors receiving recombinant human granulocyte colony-stimulating factor (rhG-CSF).
  
  
  METHODS
  Healthy donors (n = 30) of HLA-mismatched or -matched stem cell transplants were mobilized with rhG-CSF (8 microg/Kg body weight subcutaneously twice daily until completion of leukapheresis). PBPC collections were started after 4 days of rhG-CSF therapy.
  
  
  RESULTS
  Steady-state incidence of bone marrow LTC-IC, but not committed progenitors, significantly correlated with the numbers of mobilized CD34+ cells (r = 0.6, p = 0.004), CFU-GM (r = 0.79, p = 0.0005) and CFC (r = 0.76, p = 0.001) detected after 4 days of rhG-CSF therapy. Statistically significant correlations were also found between steady-state blood CFU-GM and peak numbers of CD341 cells (r = 0.68, p = 0.001), numbers of day 4 CD341 cells (r = 0.52, p = 0.005), CFU-GM (r = 0.63, p = 0.002), and CFC (r = 0.61, p = 0.003).
  
  
  CONCLUSIONS
  Our data show that in normal volunteers baseline marrow LTC-IC and blood CFU-GM correlate with rhG-CSF-mobilized PBPC. The potential clinical relevance of these findings in the identification of poor mobilizers will be tested in a prospective study.
authors.affiliation: Department of Hematology, University of Parma, Italy. ccs@ipruniv.cce.unipr.it
authors.forename: C
authors.initials: C
authors.lastname: Carlo-Stella
authors.forename: C
authors.initials: C
authors.lastname: Cesana
authors.forename: E
authors.initials: E
authors.lastname: Regazzi
authors.forename: F
authors.initials: F
authors.lastname: Falzetti
authors.forename: F
authors.initials: F
authors.lastname: Aversa
authors.forename: V
authors.initials: V
authors.lastname: Rizzoli
authors.forename: M
authors.initials: M
authors.lastname: Martelli
authors.forename: A
authors.initials: A
authors.lastname: Tabilio
mesh_terms: D000328:Adult; D001782:Blood Donors; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D014184:Transplantation, Homologous
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor