title: EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans.
abstract: BACKGROUND
  Cell-fate determination requires that cells choose between alternative developmental pathways. For example, germ cells in the nematode worm Caenorhabditis elegans choose between mitotic and meiotic division, and between oogenesis and spermatogenesis. Germ-line mitosis depends on a somatic signal that is mediated by a Notch-type signaling pathway. The ego-1 gene was originally identified on the basis of genetic interactions with the receptor in this pathway and was also shown to be required for oogenesis. Here, we provide more insight into the role of ego-1 in germ-line development.
  
  
  RESULTS
  We have determined the ego-1 gene structure and the molecular basis of ego-1 alleles. Putative ego-1 null mutants had multiple, previously unreported defects in germ-line development. The ego-1 transcript was found predominantly in the germ line. The predicted EGO-1 protein was found to be related to the tomato RNA-directed RNA polymerase (RdRP) and to Neurospora crassa QDE-1, two proteins implicated in post-transcriptional gene silencing (PTGS). For a number of germ-line-expressed genes, ego-1 mutants were resistant to a form of PTGS called RNA interference.
  
  
  CONCLUSIONS
  The ego-1 gene is the first example of a gene encoding an RdRP-related protein with an essential developmental function. The ego-1 gene is also required for a robust response to RNA interference by certain genes. Hence, a protein required for germ-line development in C. elegans may be a component of the RNA interference/PTGS machinery.
authors.affiliation: Department of Biology, Syracuse University, 108 College Place, New York 13244, USA.
authors.forename: A
authors.initials: A
authors.lastname: Smardon
authors.forename: J M
authors.initials: JM
authors.lastname: Spoerke
authors.forename: S C
authors.initials: SC
authors.lastname: Stacey
authors.forename: M E
authors.initials: ME
authors.lastname: Klein
authors.forename: N
authors.initials: N
authors.lastname: Mackin
authors.forename: E M
authors.initials: EM
authors.lastname: Maine
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics; D029742:Caenorhabditis elegans Proteins; D005718:Gametogenesis; D005854:Germ Cells; D008297:Male; D008540:Meiosis; D008938:Mitosis; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010641:Phenotype; D012323:RNA Processing, Post-Transcriptional; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
chemical_list: D029742:Caenorhabditis elegans Proteins; C406772:EGO-1 protein, C elegans; D012324:RNA-Dependent RNA Polymerase