Chemokines are produced in the brain early during the course of experimental African trypanosomiasis.
African trypanosomiasis is characterized by progressive central nervous system (CNS) involvement. Using single and double immunohistochemistry, we evaluated the induction of alpha- and beta-chemokines in brains of Sprague-Dawley rats infected with Trypanosoma brucei brucei (T. b. brucei) and identified their cellular source. The results showed high production of MIP-2, RANTES and MIP-1alpha and to a lower extend MCP-1 in infected animals compared to controls. MIP-2, RANTES and MIP-1alpha were produced early by astrocytes and microglia and later by macrophages and T-cells. These findings suggest that chemokines may contribute to the immunopathogenesis that occurs in the CNS early during infections.
Sharafeldin
A
A
Division of Infectious Diseases, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden. ahmed.sharafeldin@impi.ki.se
Eltayeb
R
R
Pashenkov
M
M
Bakhiet
M
M
D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000276:immunology / Q000378:metabolism* / Q000469:parasitology / Q000473:pathology; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000096:biosynthesis; D054426:Chemokine CXCL2; D018925:Chemokines / Q000096:biosynthesis*; D007150:Immunohistochemistry; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D017628:Microglia / Q000378:metabolism / Q000473:pathology; D015846:Monokines / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014346:Trypanosoma brucei brucei / Q000472:pathogenicity; D014353:Trypanosomiasis, African / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054426:Chemokine CXCL2; D018925:Chemokines; D019402:Macrophage Inflammatory Proteins; D015846:Monokines