Identification of a novel allele, B*15012, by polymerase chain reaction using sequence-specific primers (PCR-SSP) and sequence-based typing.
We report here a new allele, B*15012, identified by polymerase chain reaction using sequence-specific primers (PCR-SSP) and sequence-based typing (SBT). B*15012 differs to B*15011 (previously named B*1501) by a single nucleotide, at position 435 of codon 120. B*15011 encodes AAG and B*15012 encodes AAA at codon 120, this difference does not result in an amino acid change.
Day
S
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UKTSSA, Stoke Gifford, Bristol, UK.
van Dam
M
M
Buckland
E
E
Dunn
P P
PP
Ross
J
J
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D015235:HLA-B Antigens / Q000235:genetics*; D059910:HLA-B15 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D015235:HLA-B Antigens; D059910:HLA-B15 Antigen