Detection of ischemic changes in the cytoplasm of neurocytes from rat brain and spinal cord by densitometric measurement of methylene blue binding.
Ischemic changes in neurocytes from brain and spinal cord of rats were studied by densitometric measurement of bound basic stain--methylene blue. Statistically significant differences in integrated optical density (I.O.D.) of cytoplasm near to cell nucleus in brain and spinal cord neurocytes were detected after ischemia. After 10 minutes of ischemia, the average values of I.O.D. decreased to 65% and to 69.9% of I.O.D. values of controls. After 2 hours of ischemia, the average values of I.O.D. in brain cell cytoplasm reached only 43.6% and in the spinal cord cells they fell to 54.5% of control values.
Guller
L
L
Department of Anatomy, School of Medicine, Comenius University, Bratislava, Slovakia. guller@fmed.uniba.sk
Benuska
J
J
Mráz
P
P
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D003593:Cytoplasm / Q000378:metabolism / Q000473:pathology; D003720:Densitometry; D008751:Methylene Blue / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D020760:Spinal Cord Ischemia / Q000378:metabolism* / Q000473:pathology
D008751:Methylene Blue