title: Aetiology of visceral leishmaniasis in Mexico.
abstract: Two children with visceral leishmaniasis (VL), were studied by DNA analysis. DNA from liver biopsy samples from both patients, was amplified by PCR with broad primers specific for the Leishmania subgenus. DNA from the patient from Chiapas was also amplified with primers specific for the Leismania donovani complex and hybridised with a probe specific for L. donovani complex. The second patient, who is the first reported case of visceral leishmaniasis in the Mexican state of Tabasco, where localised cutaneous leishmaniasis and DCL predominate, had a co-infection with Toxoplasma gondii. The DNA from this patient was not amplified with primers specific for the L. donovani complex, did not hybridise with a probe specific for the L. donovani complex, but did hybridise with kDNA from a Mexican Leishmania mexicana strain used as a probe. We therefore, suggest that members of the L. donovani or L. mexicana complexes cause VL in Mexico.
authors.affiliation: E.N.C.B. Departamento de Inmunologia, IPN. Carpio y Plan de Ayala, Mexico City, Mexico. amonroy@alqimia.encb.ipn.mx
authors.forename: A
authors.initials: A
authors.lastname: Monroy-Ostria
authors.forename: O
authors.initials: O
authors.lastname: Hernandez-Montes
authors.forename: D C
authors.initials: DC
authors.lastname: Barker
mesh_terms: D000818:Animals; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis; D006801:Humans; D007223:Infant; D007891:Leishmania / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007893:Leishmania donovani / Q000235:genetics; D018314:Leishmania infantum / Q000235:genetics; D007894:Leishmania mexicana / Q000235:genetics; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D008099:Liver / Q000469:parasitology; D008297:Male; D008800:Mexico; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan