Virulence factors and genetic relatedness of Escherichia coli strains isolated from pigs with post-weaning diarrhea.
Forty-six Escherichia coli strains isolated from post-weaning diarrhea of pigs were analysed for their phenotypic and genotypic properties. The isolates were of serogroups O138, O139, and O141 and most of them possessed hemolytic activities. PCR analysis showed that 34 of the isolates harboured the genes for shiga toxin 2e and 32 strains possessed the genes for heat-stable enterotoxins I and II. Ten strains had the fedA gene of F18 fimbriae. The genetic relationships among all isolates were tested by random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) PCR analyses. Using the RAPD test with two different primers, six fingerprints were distinguished whereas the ERIC analysis revealed only three DNA patterns. Some strains possessing identical phenotypic and genotypic virulence determinants exhibited distinct RAPD profiles and some isolates with different pathogenic markers showed the same RAPD and ERIC pictures. Thus, RAPD, and to a less extent ERIC techniques, revealed intra- and interserogroup genotypic variations among the E. coli strains analyzed.
Osek
J
J
National Veterinary Research Institute, Department of Microbiology, Pulawvy, Poland. josek@piwet.pulawy.pl
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000737:chemistry; D001427:Bacterial Toxins / Q000235:genetics; D016384:Consensus Sequence / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000235:genetics; D019081:O Antigens / Q000737:chemistry / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D022603:Shiga Toxins; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D019081:O Antigens; D022603:Shiga Toxins