Phosphorylated MAP-1B isoforms in the developing mouse barrel cortex.
Developmental expression of two phosphorylation modes of microtubule-associated protein 1B (MAP-1B) has been studied in the barrel cortex of mice at postnatal days (P)5, P12, P21 and P90 using immunocytochemistry with antibodies 125 and 150 that recognize phosphorylation modes II and I, respectively. The antibody 125 immunoreactive processes, identified as dendrites, are not yet detectable at P5; they are already present at P12 and become more evident at P21. In the barrel cortex of P90 animals the antibody 125 immunopositive dendrites are still present, although they are much less pronounced. The antibody 150 punctate immunostaining seen at P5 is not detectable at P12. At P21, however, thin immunopositive fibres appear, implicating a re-expression of the microtubule-associated protein 1B phosphorylation mode I in a portion of axons. The antibody 150 immunopositive axons are no longer present in the P90 barrel cortex. The re-expression of the MAP-1B phosphorylation mode I, which is a juvenile isoform characteristic for growing axons, may imply induction of mechanisms providing mouse barrel cortex neurons with the potency for plastic changes at a terminal stage of synaptogenesis.
Majewska
B
B
Nencki Institute of Experimental Biology, Department of Neurophysiology, 3 Pasteur St, 02-093, Warsaw, Poland. majewska@nencki.gov.pl
Skangiel-Kramska
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003423:Critical Period, Psychological; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D016501:Neurites / Q000737:chemistry / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D010766:Phosphorylation; D013003:Somatosensory Cortex / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*; D014738:Vibrissae / Q000294:innervation
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C113540:MAP1-B protein, Cowdria ruminantium; D008565:Membrane Proteins