Structure, measurement, and secretion of human glucagon-like peptide-2.
By using radioimmunoassays toward the cDNA-predicted amino acid sequence of human glucagon-like peptide-2, a peptide was isolated from extracts of human ileum. By mass spectrometry and Edman sequencing, this peptide was identified as human proglucagon 126-158. High-performance liquid chromatography analyses indicated that a similar immunoreactive peptide (iGLP-2) was present in human plasma. Human plasma concentrations of iGLP-2 were elevated 3- to 4-fold at 1 to 2 h after ingestion of 800 to 1200 kcal meals.
Hartmann
B
B
Department of Medical Physiology, The Panum Institute, University of Copenhagen, DK-2200, Copenhagen, Denmark.
Johnsen
A H
AH
Orskov
C
C
Adelhorst
K
K
Thim
L
L
Holst
J J
JJ
D002851:Chromatography, High Pressure Liquid; D005934:Glucagon / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D053767:Glucagon-Like Peptide 2; D004763:Glucagon-Like Peptides; D006801:Humans; D007082:Ileum / Q000737:chemistry; D013058:Mass Spectrometry; D010179:Pancreas / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D052336:Proglucagon; D011498:Protein Precursors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods
D053767:Glucagon-Like Peptide 2; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D052336:Proglucagon; D004763:Glucagon-Like Peptides; D005934:Glucagon