Characterization of a 5'-flanking region supporting the transcription of mouse thymosin beta-4 in mouse NIH3T3 cells.
Expression of the gene coding for thymosin beta-4 (Tbeta-4), the major G-actin sequestering peptide in the cell, is regulated mainly at the level of transcription. In this study, we examined the nucleotide sequence of the 5'-flanking region (from -2202 to -881) of the mouse Tbeta-4 gene, and demonstrated that the DNA fragment from -278 to +410 of this gene was capable of directing the expression of a chloramphenicol acetyltransferase reporter gene in NIH3T3 cells. However, expression of the reporter gene in cells cannot be induced by interferon-alpha treatment even though a rapid activation of endogenous Tbeta-4 gene by this cytokine was observed. These results suggest that the projected interferon-stimulated response element (ISRE) might reside in other parts of the mouse Tbeta-4 gene.
Su
Y
Y
Institute of Pharmacology, College of Life Science, National Yang-Ming University, Shih-Pai, Taipei, Taiwan, Republic of China.
Chang
S L
SL
Hsiao
H L
HL
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D016898:Interferon-alpha / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013947:Thymosin / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*; D000099201:Thymosin beta(4)
D015500:Chloramphenicol O-Acetyltransferase; D004247:DNA; D016898:Interferon-alpha; D013947:Thymosin; D000099201:Thymosin beta(4)