Clink, a nanovirus-encoded protein, binds both pRB and SKP1.
Clink, a 20-kDa protein of faba bean necrotic yellows virus, a single-stranded DNA plant virus, interacts with pRB family members and a SKP1 homologue from Medicago sativa. An LxCxE motif and an F-box of Clink mediate the interactions with the respective proteins. The capacity of Clink to bind pRB correlates with its ability to stimulate viral replication. Interaction of a single protein with the cell cycle regulator pRB and SKP1, a constituent of the ubiquitin-protein turnover pathway, appears to be a novel feature. Hence, Clink may represent a new class of viral cell cycle modulators.
Aronson
M N
MN
Institut des Sciences Végétales, CNRS, 91198 Gif sur Yvette, France.
Meyer
A D
AD
Györgyey
J
J
Katul
L
L
Vetten
H J
HJ
Gronenborn
B
B
Timchenko
T
T
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D016160:Retinoblastoma Protein / Q000378:metabolism*; D044786:S-Phase Kinase-Associated Proteins; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C406062:Clink protein, faba bean necrotic yellows virus; D004279:DNA, Viral; D016160:Retinoblastoma Protein; D044786:S-Phase Kinase-Associated Proteins; D014764:Viral Proteins