Protective effects of soyasapogenol A on liver injury mediated by immune response in a concanavalin A-induced hepatitis model.
The present study was carried out to analyze the effects of soyasapogenol A on the liver injury mediated by the immune response in concanavalin A-induced hepatitis in mice. Soyasapogenol A reduced the number of infiltrating inflammatory cells in the liver and significantly lowered the elevated level of plasma tumor necrosis factor-alpha (TNF-alpha) 2 h after concanavalin A treatment, and then markedly reduced the elevated plasma alanine aminotransferase activity and decreased the number of apoptotic bodies in the liver parenchymal cells but not in the sinusoidal cells at 24 h. Since the effect of soyasapogenol A on the elevated plasma TNF-alpha level was not appreciable compared to the preventive effect of soyasapogenol A on the elevated plasma alanine aminotransferase level, these results suggest that soyasapogenol A directly prevents apoptosis of hepatocytes, and secondly, inhibits the elevation of plasma TNF-alpha, which consequently resulted in the prevention of liver damage in the concanavalin A-induced hepatitis model.
Kuzuhara
H
H
Drug Discovery, Pharmaceutical Research Center, Meiji Seika, 760 Morooka, Kouhoku, Yokohama, Japan.
Nishiyama
S
S
Minowa
N
N
Sasaki
K
K
Omoto
S
S
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001219:Aspartate Aminotransferases / Q000097:blood; D003208:Concanavalin A; D016207:Cytokines / Q000097:blood; D006520:Hepatitis, Animal / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use
D016207:Cytokines; D003208:Concanavalin A; C116604:soyasapogenol A; D009828:Oleanolic Acid; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase