The utilization of 4-aminobutylphosphonate as sole nitrogen source by a strain of Kluyveromyces fragilis.
A strain of the yeast Kluyveromyces fragilis was screened for its ability to utilize a range of synthetic and natural organophosphonate compounds as the sole source of phosphorus, nitrogen or carbon. Only 4-aminobutylphosphonate was utilized as sole nitrogen source with protein yields increasing proportionally with substrate concentrations up to 10 mM. No 4-aminobutylphosphonate metabolizing enzyme activity was detectable in cell-free extracts prepared from K. fragilis pregrown on 2.5 mM 4-aminobutylphosphonate. None of the organophosphonates tested served as a source of carbon or phosphorus for K. fragilis.
Ternan
N G
NG
School of Environmental Studies, University of Ulster, Coleraine, UK. ng.ternan@ulst.ac.uk
McMullan
G
G
D001673:Biodegradation, Environmental; D002082:Butylamines / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D007716:Kluyveromyces / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*
D002082:Butylamines; D003470:Culture Media; D009943:Organophosphorus Compounds; C079360:4-aminobutylphosphonic acid; D009584:Nitrogen