Crystallization and preliminary X-ray study of beta--mannosidase from Trichoderma reesei.
beta-Mannosidase from Trichoderma reesei, a 105 kDa glycoprotein, has been crystallized. The crystals belong to the space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell dimensions a = b = 165.86, c = 122.46 A, and diffract beyond 2.75 A resolution. X-ray diffraction data were collected from a frozen crystal on a synchrotron X-ray source.
Aparicio
R
R
National Synchrotron Light Laboratory, LNLS, Caixa Postal 6192, CEP 13083-970, Campinas, SP, Brazil.
Eneiskaya
E V
EV
Kulminskaya
A A
AA
Savel'ev
A N
AN
Golubev
A M
AM
Neustroev
K N
KN
Kobarg
J
J
Polikarpov
I
I
D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008361:Mannosidases / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D011487:Protein Conformation; D014242:Trichoderma / Q000201:enzymology*; D044902:beta-Mannosidase
D005656:Fungal Proteins; D008361:Mannosidases; D044902:beta-Mannosidase