title: Molecular cloning and expression analysis of a putative nuclear protein, SR-25.
abstract: We cloned a full-length mouse cDNA and its human homologue encoding a novel protein designated as "SR-25." In Northern blot analysis, SR-25 mRNA was expressed in all organs tested, and relatively abundant in testis and thymus. Deduced amino acid sequences of mouse SR-25 and human SR-25 showed 77.7% identity. SR-25 has a serine-arginine repeat (SR repeat) and two types of amino acid clusters: a serine cluster and a highly basic cluster. Based on the presence of many nuclear localizing signals and a similarity to RNA splicing proteins, SR-25 is strongly suggested to be a nuclear protein and may contribute to RNA splicing.
authors.affiliation: Department of Pediatrics, School of Medicine, Tokushima, 770-8503, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sasahara
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
authors.forename: M
authors.initials: M
authors.lastname: Moritani
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Iwahana
authors.forename: K
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: J
authors.initials: J
authors.lastname: Miyagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kuroda
authors.forename: M
authors.initials: M
authors.lastname: Itakura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: C406330:ARL6IP4 protein, human; C485788:Arl6ip4 protein, mouse; D018076:DNA, Complementary; D009687:Nuclear Proteins; D012333:RNA, Messenger