Tissue-specific expression of a type I adenylyl cyclase rescues the rutabaga mutant memory defect: in search of the engram.
Most attempts to localize physical correlates of memory in the central nervous system (CNS) rely on ablation techniques. This approach has the limitation of defining just one of an unknown number of structures necessary for memory formation. We have used the Drosophila rutabaga type I Ca(2+)/CaM-dependent adenylyl cyclase (AC) gene to determine in which CNS region AC expression is sufficient for memory formation. Using pan-neural and restricted CNS expression with the GAL4 binary transcription activation system, we have rescued the memory defect of the rutabaga mutant in a fast robust spatial learning paradigm. The ventral ganglion, antennal lobes, and median bundle are likely the CNS structures sufficient for rutabaga AC- dependent spatial learning.
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Theodor Boveri Institut fuer Biowissenschaften, Lehrstuhl fuer Genetik, (Biozentrum) Am Hubland, D97074, Wuerzburg, Germany.
Wolf
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Davis
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Heisenberg
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D000262:Adenylyl Cyclases / Q000096:biosynthesis* / Q000235:genetics; D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning / Q000502:physiology; D001522:Behavior, Animal / Q000502:physiology; D002490:Central Nervous System / Q000201:enzymology* / Q000502:physiology; D003216:Conditioning, Operant / Q000502:physiology; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000032:analysis*; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D004742:Enhancer Elements, Genetic; D005260:Female; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D017952:Ganglia, Invertebrate / Q000378:metabolism; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D008568:Memory / Q000502:physiology*; D016296:Mutagenesis; D009928:Organ Specificity / Q000235:genetics / Q000502:physiology; D010641:Phenotype; D029701:Saccharomyces cerevisiae Proteins; D013037:Spatial Behavior / Q000502:physiology; D013696:Temperature; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000262:Adenylyl Cyclases; C106670:adenylyl cyclase 1