title: Effect of maturation on nerve excitability in an experimental model of threshold electrotonus.
abstract: Threshold electrotonus (TE) is a new tool for investigating axonal function noninvasively in vivo. To increase its potential clinical value, we developed a rat model of TE, and examined the effects of maturation and pharmacological intervention. We recorded TE in 92 male rats (body weight 90-650 g) by stimulating the motor nerve in the tail, and applying 100-ms conditioning currents. Motor conduction velocities increased up to a body weight of 330 g, and remained constant thereafter. TE in mature rats was similar to that in humans, and two parameters were analyzed: TEd(10-20) or the mean threshold reduction 10-20 ms after the onset of the depolarizing conditioning current at 40% of threshold intensity; and TEh(10-20) or the corresponding threshold decrease on hyperpolarization. Like latency, the absolute value of TEh(10-20) decreased up to 330 g, and then stabilized thereafter, probably reflecting the progressive increase in the axonal diameter and relative reduction in internodal impedance. In contrast, TEd(10-20) gradually decreased up to 330 g, and then jumped to a higher level, which was maintained for animals of >400 g. 4-Aminopyridine, a blocker of fast potassium channels, selectively increased TEd(10-20) only in the immature or young (<330 g) rats. This suggests that, in the mature animals, fast potassium channels become sequestrated from the nodal membrane and not activated in response to nodal depolarization. These findings indicate that mature rats (>400 g) may provide a useful experimental model for interpreting abnormal TE responses in humans, and provide evidence for nonlinear maturation of potassium channel function in myelinated axons.
authors.affiliation: Department of Neurology, Kyoto University Faculty of Medicine, Shogoin Sakyoku, Kyoto 606-8507, Japan.
authors.forename: Q
authors.initials: Q
authors.lastname: Yang
authors.forename: R
authors.initials: R
authors.lastname: Kaji
authors.forename: N
authors.initials: N
authors.lastname: Hirota
authors.forename: Y
authors.initials: Y
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Takagi
authors.forename: N
authors.initials: N
authors.lastname: Kohara
authors.forename: J
authors.initials: J
authors.lastname: Kimura
authors.forename: H
authors.initials: H
authors.lastname: Shibasaki
authors.forename: H
authors.initials: H
authors.lastname: Bostock
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000375:Aging; D000818:Animals; D002586:Cesium / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004558:Electric Stimulation; D004594:Electrophysiology / Q000379:methods*; D006801:Humans; D008297:Male; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D024510:Muscle Development; D009129:Muscle Tonus; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000294:innervation / Q000502:physiology; D009431:Neural Conduction / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D012684:Sensory Thresholds / Q000502:physiology*; D013623:Tail / Q000294:innervation
chemical_list: D002712:Chlorides; D002586:Cesium; D015761:4-Aminopyridine; C028019:cesium chloride