Use of rep-PCR to define genetic relatedness among Bacteroides fragilis strains.
Bacteroides fragilis, a component of the normal flora and an important anaerobic pathogen in non-intestinal endogenous infections, has recently been associated with enteric diseases. In this study, 41 B. fragilis strains were analysed in relation to their genetic diversity. This collection included two reference strains (ATCC 23745 and 25285), 20 isolates from non-intestinal infections, six from intestinal infections, five from intestinal microflora and eight from an aquatic environment. The fingerprints were generated by using two repetitive sequences (REP and ERIC) as primers to PCR (rep-PCR). A dendrogram was obtained with the Taxotron Program. Three clusters (threshold genotypes I, II and III) were observed when the genetic distance was 0.30. These results confirm previous data found regarding the genotypical diversity of B. fragilis.
Moraes
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Gonçalves
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Instituto de Microbiologia Prof. Paulo de Góes, UFRJ, Rio de Janeiro, Brasil *Faculdade de Odontologia de Piracicaba/UNICAMP, Brasil and †Faculté de Medicine Dentaire, Universite Laval, Quebec, Canada.
Mouton
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Instituto de Microbiologia Prof. Paulo de Góes, UFRJ, Rio de Janeiro, Brasil *Faculdade de Odontologia de Piracicaba/UNICAMP, Brasil and †Faculté de Medicine Dentaire, Universite Laval, Quebec, Canada.
Seldin
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Ferreira
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Domingues
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RMCP
D001442:Bacteroides Infections / Q000382:microbiology*; D001441:Bacteroides fragilis / Q000145:classification* / Q000235:genetics; D016000:Cluster Analysis; D016384:Consensus Sequence; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000737:chemistry*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007422:Intestines / Q000382:microbiology; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D014871:Water Microbiology
D004269:DNA, Bacterial