Real-time quantification of human telomerase reverse transcriptase mRNA in tumors and healthy tissues.
BACKGROUND
Expression of the hTERT gene, which codes for the catalytic subunit of telomerase, is associated with malignancy. We recently developed a real-time reverse transcription-PCR assay, based on TaqMan technology, for accurate and reproducible determination of hTERT mRNA expression (Lab Investig 1999;79:911-2). This method may be of interest for molecular tumor diagnostics in tissues and corresponding body fluids, washings, or brushes.


METHODS
In this study, we measured hTERT expression in a subset of healthy tissues and tumors to select those tumor types with the best potential for quantification of hTERT in corresponding body fluids. To demonstrate the use of the method in body fluids, we quantified hTERT expression in voided urine of patients with bladder cancer and controls.


RESULTS
Real-time measurement of hTERT expression could discriminate between all healthy and malignant tissue samples from pancreas, lung, esophagus, and bladder, but not for colon tissues. Moreover, in five of nine (55%) urine samples, hTERT could be quantified.


CONCLUSIONS
The present study demonstrates that accurate quantitative measurement of hTERT expression has high potential for discrimination between healthy and tumor cells in tissues and urine and supports future measurements in pancreatic fluid, bronchoalveolar lavage fluid, esophageal brushings, and urine or bladder washings.
de Kok
J B
JB
Departments of Clinical Chemistry, Surgery, Pathology, and Urology, University Hospital Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. J.dekok@ckcl.azn.nl
Ruers
T J
TJ
van Muijen
G N
GN
van Bokhoven
A
A
Willems
H L
HL
Swinkels
D W
DW
D004268:DNA-Binding Proteins; D006801:Humans; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012313:RNA; D012333:RNA, Messenger / Q000032:analysis* / Q000652:urine; D019098:Telomerase / Q000235:genetics* / Q000652:urine; D001749:Urinary Bladder Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000652:urine
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase