title: Simultaneous spectrophotometric and volumetric determinations of amoxycillin, ampicillin and cloxacillin in drug formulations: reaction mechanism in the base catalysed hydrolysis followed by oxidation with iodate in dilute acid solution.
abstract: A method for the analysis of degraded products of amoxycillin, ampicillin and cloxacillin in drug formulations, obtained as a result of their base hydrolysis is described. Simultaneous spectrophotometric and volumetric determinations of the antibiotic is based on the neutralization of the degraded product by dilute hydrochloric acid to get a pH approximately 2 to be conducive for redox titration using potassium iodate as titrant. A red purple colour is developed in carbon tetrachloride at the end point. Spectrophotometry is done after separating the organic layer and measuring the absorbance of red-purple colour at lambda(max) 520 nm. The pathways of different degraded products and their oxidation mechanism is described on IR, TLC and UV spectroscopic studies.
authors.affiliation: Department of Chemistry, Analytical Research Laboratory, Aligarh Muslim University, India.
authors.forename: S Z
authors.initials: SZ
authors.lastname: Qureshi
authors.forename: T
authors.initials: T
authors.lastname: Qayoom
authors.forename: M I
authors.initials: MI
authors.lastname: Helalet
mesh_terms: D000143:Acids / Q000737:chemistry; D000658:Amoxicillin / Q000032:analysis; D000667:Ampicillin / Q000032:analysis; D002384:Catalysis; D002626:Chemistry, Pharmaceutical; D002855:Chromatography, Thin Layer / Q000379:methods*; D003023:Cloxacillin / Q000032:analysis; D006868:Hydrolysis; D007452:Iodates / Q000737:chemistry; D010084:Oxidation-Reduction; D010406:Penicillins / Q000032:analysis*; D013055:Spectrophotometry, Infrared / Q000379:methods
chemical_list: D000143:Acids; D007452:Iodates; D010406:Penicillins; D000667:Ampicillin; D000658:Amoxicillin; D003023:Cloxacillin