A T-extended vector using a green fluorescent protein as an indicator.
T-extended vector (T-vector) is a useful tool for cloning PCR products directly. We exploited a novel T-vector using a green fluorescent protein (GFP) as an indicator based on insertional inactivation. The brightest GFP mutant was used for easy detection even under daylight. The 100bp and 0.9kb of PCR products were cloned, and the transformant colonies with inserts were adjudged by the fluorescent green-white screening. The GFP system was more sensitive to insertional inactivation than the beta-galactosidase system at the conventional insertion sites.
Ito
Y
Y
Department of Functional Materials Science, Saitama University, Urawa, Japan.
Suzuki
M
M
Husimi
Y
Y
D001483:Base Sequence; D002999:Clone Cells; D004926:Escherichia coli / Q000235:genetics; D005453:Fluorescence; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000737:chemistry; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014170:Transformation, Genetic
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins