Protection of mitochondrial functions against oxidative stresses by isoflavans from Glycyrrhiza glabra.
Isoflavan derivatives, glabridin (1), hispaglabridin A (2), hispaglabridin B (3), 4'-Omethylglabridin (4) and 3'-hydroxy-4'-O-methylglabridin (5), isolated from Glycyrrhiza glabra, were investigated for their ability to protect liver mitochondria against oxidative stresses. Mitochondrial lipid peroxidation linked to respiratory electron transport and that induced non-enzymatically were inhibited by these isoflavans. Hispaglabridin A (2) strongly inhibited both peroxidations and 3'-hydroxy-4'-O-methylglabridin (5) was the most effective at preventing NADH-dependent peroxidation. 3'-Hydroxy-4'-O-methylglabridin (5) protected mitochondrial respiratory enzyme activities against NADPH-dependent peroxidation injury. Dihydroxyfumarate-induced mitochondrial peroxidation was also prevented by this isoflavan. Isoflavans from G. glabra were shown to be effective in protecting mitochondrial function against oxidative stresses.
Haraguchi
H
H
Faculty of Engineering, Fukuyama University, Japan.
Yoshida
N
N
Ishikawa
H
H
Tamura
Y
Y
Mizutani
K
K
Kinoshita
T
T
D000818:Animals; D006035:Glycyrrhiza / Q000737:chemistry*; D007529:Isoflavones / Q000494:pharmacology*; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress; D010636:Phenols / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar
D007529:Isoflavones; D010636:Phenols; C107601:glabridin