title: Equistatin, a protease inhibitor from the sea anemone actinia equina, is composed of three structural and functional domains.
abstract: A cDNA encoding a precursor of equistatin, a potent cysteine and aspartic proteinase inhibitor, was isolated from the sea anemone Actinia equina. The deduced amino acid sequence of a 199-amino-acid residue mature protein with 20 cysteine residues, forming three structurally similar thyroglobulin type-1 domains, is preceded by a typical eukaryotic signal peptide. The mature protein region and those coding for each of the domains were expressed in the periplasmic space of Escherichia coli, isolated, and characterized. The whole recombinant equistatin and its first domain, but not the second and third domains, inhibited the cysteine proteinase papain (K(i) 0.60 nM) comparably to natural equistatin. Preliminary results on inhibition of cathepsin D, supported by structural comparison, show that the second domain is likely to be involved in activity against aspartic proteinases.
authors.affiliation: Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Jamova 39, Ljubljana, SI-1000, Slovenia. borut.strukelj@ijs.si
authors.forename: B
authors.initials: B
authors.lastname: Strukelj
authors.forename: B
authors.initials: B
authors.lastname: Lenarcic
authors.forename: K
authors.initials: K
authors.lastname: Gruden
authors.forename: J
authors.initials: J
authors.lastname: Pungercar
authors.forename: B
authors.initials: B
authors.lastname: Rogelj
authors.forename: V
authors.initials: V
authors.lastname: Turk
authors.forename: D
authors.initials: D
authors.lastname: Bosch
authors.forename: M A
authors.initials: MA
authors.lastname: Jongsma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D012615:Sea Anemones / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*
chemical_list: D011506:Proteins; D011994:Recombinant Proteins; D015842:Serine Proteinase Inhibitors; C106356:equistatin