Enhancement of the transformed shape phenotype by microtubule inhibitors and reversal by an inhibitor combination.
Differences between transformed cells and their normal counterparts were defined by analyzing the cells' three-dimensional distribution of mass. Variables called factors, which explained the covariance of the real variables, were extracted from the data. We found that factors #4 (sharp, tapering projections) and #12 (rounding up), corresponded to G-protein functions. Then, the signature-type mass distribution of transformed cells was defined by factor values. Agents that caused signature-type mimicry could quantitatively shift factor values, for example, those affecting endocytic processing disproportionately reduced values of #4. Signature-type reversal was also observed and may be valuable in predicting the efficacy of chemotherapeutic agents.
Heckman
C A
CA
Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403-0212, USA.
Plummer
H K
HK
Mukherjee
R
R
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003078:Colchicine / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects*; D008985:Monensin / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D010641:Phenotype; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
D007328:Insulin; D014634:Valinomycin; D008985:Monensin; D013755:Tetradecanoylphorbol Acetate; D017239:Paclitaxel; D003078:Colchicine