Adaptive responses of human monocytes infected by bordetella pertussis: the role of adenylate cyclase hemolysin.
The activation/adaptive responses of human monocytes exposed to Bordetella pertussis parental or mutant strains were evaluated and correlated to the expression of two bacterial toxins: adenylate cyclase-hemolysin and pertussis toxin. The marked rise in intracellular cyclic adenosine monophosphate (cAMP) observed in monocytes infected by B. pertussis parental strain, inversely correlated with (1) the production of tumor necrosis factor alpha; (2) the release of superoxide anion; and (3) the expression of the 72-kDa heat shock/stress protein, Hsp70. Experiments performed with mutants deficient in adenylate cyclase-hemolysin or with purified bacterial toxins confirmed the key role of adenylate cyclase-hemolysin in the control of monocytes' response to infection by B. pertussis. This bacterial strategy primarily involves evasion from antimicrobial defenses and, eventually, the sacrifice of the host cell.
Njamkepo
E
E
Laboratoire des Bordetella, Institut Pasteur, Paris, France.
Pinot
F
F
François
D
D
Guiso
N
N
Polla
B S
BS
Bachelet
M
M
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins / Q000097:blood* / Q000633:toxicity; D001886:Bordetella pertussis / Q000472:pathogenicity*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000097:blood; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000235:genetics; D006460:Hemolysin Proteins / Q000097:blood* / Q000633:toxicity; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008070:Lipopolysaccharides / Q000633:toxicity; D009000:Monocytes / Q000382:microbiology* / Q000502:physiology* / Q000648:ultrastructure; D011498:Protein Precursors / Q000097:blood* / Q000633:toxicity; D013481:Superoxides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D018840:HSP70 Heat-Shock Proteins; D006460:Hemolysin Proteins; D008070:Lipopolysaccharides; D011498:Protein Precursors; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D000242:Cyclic AMP; D007770:L-Lactate Dehydrogenase