title: In vivo effects of olive oil-based lipid emulsion on lymphocyte activation in rats.
abstract: Numerous studies suggest that immune function may be compromised by lipid emulsions rich in polyunsaturated fatty acids, especially linoleic acid. In our study, we compared the effect of a new olive oil-based lipid emulsion (ClinOleic(R)) containing 18% linoleic acid, and an emulsion based on soybean oil (Ivelip(R); 52% linoleic acid) on lymphocyte functions. Weaning Wistar rats (n= 24) were fed for 4 weeks on an oral diet that contained 12% of total energy as lipids from soybean oil. Then they received, during 6 days, a total parenteral nutrition (260 kcal/kg/d) in which 12% of total energy was brought by one of the two lipid emulsions. The fatty acid profile of spleen lymphocyte phospholipids reflected lipid intakes, with a higher content of oleic acid in ClinOleic(R) group and linoleic acid in Ivelip(R) group. A greater proportion of cells expressed the interleukin-2 receptor a-chain (CD25) after administration of ClinOleic(R) when compared to Ivelip(R) (55.43 +/- 3.47 vs 45.48 +/- 3.26%, P<< 0.05). Moreover, the CD25 expression was positively correlated with oleic acid content of spleen lymphocyte phospholipids (r= 0.500, P<< 0.018). These results show that ClinOleic(R) is able to induce, in vivo, a greater proportion of cells expressing CD25, and suggest that oleic acid could have a role in the observed effects.
authors.affiliation: Groupe d'Etudes en Nutrition Infantile, Toulouse, France.
authors.forename: M
authors.initials: M
authors.lastname: Moussa
authors.forename: J
authors.initials: J
authors.lastname: Le Boucher
authors.forename: J
authors.initials: J
authors.lastname: Garcia
authors.forename: J
authors.initials: J
authors.lastname: Tkaczuk
authors.forename: J
authors.initials: J
authors.lastname: Ragab
authors.forename: G
authors.initials: G
authors.lastname: Dutot
authors.forename: E
authors.initials: E
authors.lastname: Ohayon
authors.forename: J
authors.initials: J
authors.lastname: Ghisolfi
authors.forename: J P
authors.initials: JP
authors.lastname: Thouvenot
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D007376:Interleukin-2 / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D016131:Lymphocyte Subsets; D008214:Lymphocytes / Q000737:chemistry / Q000276:immunology; D008297:Male; D000069463:Olive Oil; D010289:Parenteral Nutrition, Total; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology
chemical_list: D004042:Dietary Fats, Unsaturated; D005217:Fat Emulsions, Intravenous; D005227:Fatty Acids; D007376:Interleukin-2; D000069463:Olive Oil; D010938:Plant Oils