Lobular distribution pattern of lactate dehydrogenase and 6-phosphogluconate dehydrogenase activity in rat liver.
Lactate dehydrogenase (LDH) and 6-phosphogluconate dehydrogenase (6-PGDH) activities were measured in lobular areas expanding between 3 portal tracts and an efferent central vein in the livers of male Wistar rats, using a Lowry technique. The maximum of LDH activity was found in a nearly uniform broad area in the lobular periphery. From that area values decreased along periportal/septal-->perivenous gradients, but only slightly within that area along the periportal-->septal axis of the vascular septum. Maximum values of 6-PGDH activity were present in an intermediate area close to the central vein demonstrating a rather inhomogeneous distribution pattern without a clear definition of zonal limits. Our data on the distribution pattern of LDH are in agreement with the concept of the metabolic lobulus and are supported by a recent evaluation of the vascular architecture in rat liver. The lobular distribution pattern of 6-PGDH cannot be interpreted without doubt in accordance with that concept.
Schmidt
B
B
Institut für Anatomie, Universität Münster, Vesaliusweg, Germany.
Vogelsang
M
M
Haubitz
I
I
Hildebrand
R
R
D000818:Animals; D006503:Hepatic Veins / Q000201:enzymology; D006651:Histocytochemistry / Q000379:methods; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D011168:Portal System / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar
D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase