A novel C-terminal kinesin subfamily may be involved in chromosomal movement in caenorhabditis elegans.
C-terminal kinesin motor proteins, such as the Drosophila NCD and yeast KAR3, are involved in chromosomal segregation. Previously we have described two orthologs of NCD in Caenorhabditis elegans, KLP-3 and KLP-17, which also participate in chromosome movement. Here we report cDNA cloning of klp-15 and klp-16, and the expression pattern of the genes encoding C-terminal motor kinesins including klp-15 and klp-16. Interestingly KLP-15 and KLP-16 form a unique class of C-terminal kinesins, distinct from the previously known C-terminal motors in other organisms. Using in situ hybridization and RNA interference assay, we show that although all of these motors mediate chromosome segregation, they do so in a combination of unique and overlapping manners, suggesting a complex hierarchy of kinesin motor function in metazoans.
Ali
M Y
MY
Laboratory of Molecular Biology, Department of Ecological Engineering, Toyohashi University of Technology, Tempaku-cho, Toyohashi, Japan.
Siddiqui
Z K
ZK
Malik
A B
AB
Siddiqui
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D020090:Chromosome Segregation; D003001:Cloning, Molecular; D018509:DNA, Helminth; D015870:Gene Expression; D015801:Helminth Proteins / Q000235:genetics / Q000502:physiology*; D017403:In Situ Hybridization; D016547:Kinesins / Q000235:genetics / Q000502:physiology*; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D018510:RNA, Helminth; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; D015801:Helminth Proteins; C407129:KLP-15 protein, C elegans; C407128:KLP-16 protein, C elegans; D008869:Microtubule-Associated Proteins; D018510:RNA, Helminth; D016547:Kinesins