Modelling of conditions for the enantiomeric separation of beta2-adrenergic sympathicomimetics by capillary electrophoresis using cyclodextrins as chiral selectors in a polyethylene glycol gel.
A two-factor central composite design was used to determine a mathematical model for prediction of the optimal conditions for the separation of the enantiomers of some widely used beta2-sympathicomimetic drugs (beta2-agonists) by capillary electrophoresis using cyclodextrins (CD) as a chiral selector in a polyethylene glycolgel. The effects of the chemical structure of these drugs along with the addition of polyethylene glycol to the cyclodextrin solution on the resolution of their enantiomers were studied. To allow impurity studies down to 0.1% (distomer eutomer) a resolution of 2.5 should be warranted. Those beta2-agonists containing two hydroxylic groups in the aromatic ring structure show the highest enantiomeric separation, due to the fact that one of their enantiomers has a better geometric structure to fit into the beta-cyclodextrin cavity.
de Boer
T
T
Department of Analytical Chemistry and Toxicology, University Center for Pharmacy, Groningen Institute for Drug Studies, The Netherlands.
Bijma
R
R
Ensing
K
K
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000737:chemistry* / Q000302:isolation & purification; D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods; D005069:Evaluation Studies as Topic; D008956:Models, Chemical; D008962:Models, Theoretical; D011092:Polyethylene Glycols / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013566:Sympathomimetics / Q000737:chemistry* / Q000302:isolation & purification
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; D003505:Cyclodextrins; D013566:Sympathomimetics; D011092:Polyethylene Glycols