Improvement purification of sulfated oligofucan by ion-exchange displacement centrifugal partition chromatography.
Centrifugal partition chromatography in ion-exchange displacement mode was used to fractionate mixtures of sulfated oligofucans obtained by partial depolymerization of brown seaweed fucoidans. Diluted (10%, v/v) protonated LA2 (a lipophilic secondary amine) is used as a weak exchanger. In an attempt to improve this method, several solvents (methyl isobutyl ketone, methyl tert.-butyl ether, BuOH) were tested to dissolve LA2H+. MtBE produced less bleeding than MiBK, whereas BuOH proved unsuitable. The sample injected needs to be highly diluted in water to ensure participation in the chromatographic process. A comparison of data (NMR, composition, molecular mass) indicated the homogeneity of the fractions obtained as well as the differences between them.
Chevolot
L
L
Unité de Recherche Marine 2: Laboratoire Biochimie et Molécules Marines, IFREMER, Nantes, France. chevolot@ifremer.fr
Foucault
A
A
Colliec-Jouault
S
S
Ratiskol
J
J
Sinquin
C
C
D002498:Centrifugation; D002852:Chromatography, Ion Exchange / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000302:isolation & purification*; D012997:Solvents
D011134:Polysaccharides; D012997:Solvents; C007789:fucoidan