Aspirin and salicylic acid do not inhibit methyl jasmonate-inducible expression of a gene for ornithine decarboxylase in tobacco BY-2 cells.
Similar to the prostanoid-mediated inflammatory response in mammals, jasmonate-mediated wound response in plant leaves is inhibited by salicylic acid (SA) or acetylsalicylate (aspirin). In tobacco BY-2 cells, expression of the gene for ornithine decarboxylase (ODC) involved in putrescine synthesis is rapidly inducible by methyl jasmonate (MeJA). A nuclear gene for ODC isolated from tobacco, gNtODC-1, was an intron-less gene and MeJA induced the expression of a GUS fusion gene with the gNtODC-1 promoter in transformed tobacco cells. Although SA alone did not induce the expression, 0.2 to 20 microM SA increased the MeJA-induced expression of the fusion gene to about two-fold. A similar increase was observed with aspirin but not with 3- or 4-hydroxybenzoic acids. SA at concentrations up to 200 microM did not inhibit the MeJA-induction of mRNAs for the GUS fusion gene and the endogenous gene for ODC.
Imanishi
S
S
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University, Japan.
Nakakita
M
M
Yamashita
K
K
Furuta
A
A
Utsuno
K
K
Muramoto
N
N
Kojima
H
H
Nakamura
K
K
D000085:Acetates / Q000494:pharmacology*; D000596:Amino Acids / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000494:pharmacology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D007192:Indenes / Q000494:pharmacology; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics*; D054883:Oxylipins; D010947:Plants, Toxic; D020156:Salicylic Acid / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology*
D000085:Acetates; D000596:Amino Acids; D001427:Bacterial Toxins; D003517:Cyclopentanes; D007192:Indenes; D054883:Oxylipins; C058487:coronatine; C072239:methyl jasmonate; D009955:Ornithine Decarboxylase; D020156:Salicylic Acid; D001241:Aspirin