Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene.
We have analyzed the DNA fragment localized about 11 to 17.5 kb upstream of the chicken alpha-globin gene domain (the fragment was designed as alpha-0). The nucleotide sequence of its 3.3 kb-long 5' part was established and interactions with nuclear matrix proteins were studied. The DNA region localized about 16 kb upstream of the embryonic pi-globin gene showed high affinity to nuclear matrices in vitro. Two palindromes and a cluster of inverted repeats were co-localized in the same region. The whole 6.6 kb alpha-0 fragment decreased the activity of linked CAT reporter gene when transfected into chicken erythroblastoid cells.
Pietrowska
M
M
Department of Experimental and Clinical Radiobiology, Center of Oncology, Gliwice, Poland.
Rusin
M
M
Widłak
P
P
Razin
S V
SV
Rzeszowska-Wolny
J
J
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002642:Chick Embryo; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004900:Erythroblasts / Q000378:metabolism; D017930:Genes, Reporter; D005914:Globins / Q000235:genetics*; D015530:Nuclear Matrix / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
D009687:Nuclear Proteins; D005914:Globins; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase