Enhancement of mitomycin C efficiency by vitamin C, E-acetate and beta-carotene under irradiation. A study in vitro.
Using E.coli bacteria (AB 1157) and leukemia cells (HL 60) as a model for in vitro studies it was established that the efficiency of mitomycin C (MMC) can be influenced in the presence of antioxidant vitamins. This synergistic effect of the vitamins C, E-acetate and beta-carotene on MMC activity is rather strong for E.coli bacteria under irradiation (15 and 50 Gy) in the presence of air. Vitamin C contributes more efficiently to the MMC-activity in leukemia cells than the other two vitamins. The effect is explained by a cascade electron transfer process from the vitamins to MMC, where vitamin C is acting as a major electron source. These results might be of importance in cancer therapy.
Kammerer
C
C
Ludwig Boltzmann Institute for Radiation Chemistry and Radiation Biology, Vienna, Austria.
Czermak
I
I
Getoff
N
N
Kodym
R
R
D001205:Ascorbic Acid / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000187:drug effects / Q000528:radiation effects; D018922:HL-60 Cells; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology*; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*; D019207:beta Carotene / Q000494:pharmacology*
D019207:beta Carotene; D014810:Vitamin E; D016685:Mitomycin; D024502:alpha-Tocopherol; D001205:Ascorbic Acid; D024505:Tocopherols