title: PLGamide characterization and role in osmoregulation in leech brain.
abstract: Neurons immunoreactive to an antiserum specifically directed against the Prolyl-Leucyl-Glycinamide peptide (PLGamide: Melanocyte Inhibiting Factor: MIF) were detected in the brain of the leech Theromyzon tessulatum. Radioimmunoassay titrations of the PLGamide-like material at different physiological stages of the life cycle indicated a maximal amount at stage 3B, which is correlated to phase of both maximal water uptake and coelomic vitellogenin accumulation. In vivo experiments demonstrate that this cerebral PLGamide-like material is an anti-diuretic factor that would act at stage 3B in order to permit a water uptake leading to water retention allowing coelomic yolk protein accumulation. In brains of the Gnatobdellid leech Hirudo medicinalis and the Pharyngobdellid leech Erpobdella octoculata, anti-PLGamide material was also detected with an amount not differing with the degree of sex maturation of the animals, confirming the link between osmoregulation and ovogenesis in rhynchobdellid leeches. Using a combination of biochemical techniques including high-pressure gel permeation chromatography followed by reversed-phase HPLC on brain extracts and Edman degradation, we demonstrated the presence of an authentic MIF-1 peptide in leech brain. Finally, since in vertebrates MIF-1 belongs to the non-classical opioid peptide family, we studied its binding displacement, in contrast to morphine, on mu-receptors and on nitric oxide (NO) release experiments in leech brain. PLGamide did not bind to mu-alkaloid opioid receptors and did not stimulate NO release.
authors.affiliation: Laboratoire d'Endocrinologie des Annélides, UPRES-A CNRS 8017, SN3, Université des Sciences et Technologies de Lille, 59650 Villeneuve d'Ascq, France. michel.salzet@univ-lille1.fr
authors.forename: M
authors.initials: M
authors.lastname: Salzet
authors.forename: L
authors.initials: L
authors.lastname: Deloffre
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007118:Immunoassay; D007865:Leeches; D008018:Life Cycle Stages; D009075:MSH Release-Inhibiting Hormone / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000378:metabolism; D014882:Water-Electrolyte Balance
chemical_list: D017450:Receptors, Opioid, mu; D009569:Nitric Oxide; D009075:MSH Release-Inhibiting Hormone