title: The sulfur-regulated arylsulfatase gene cluster of Pseudomonas aeruginosa, a new member of the cys regulon.
abstract: A gene cluster upstream of the arylsulfatase gene (atsA) in Pseudomonas aeruginosa was characterized and found to encode a putative ABC-type transporter, AtsRBC. Mutants with insertions in the atsR or atsB gene were unable to grow with hexyl-, octyl-, or nitrocatecholsulfate, although they grew normally with other sulfur sources, such as sulfate, methionine, and aliphatic sulfonates. AtsRBC therefore constitutes a general sulfate ester transport system, and desulfurization of aromatic and medium-chain-length aliphatic sulfate esters occurs in the cytoplasm. Expression of the atsR and atsBCA genes was repressed during growth with sulfate, cysteine, or thiocyanate. No expression of these genes was observed in the cysB mutant PAO-CB, and the ats genes therefore constitute an extension of the cys regulon in this species.
authors.affiliation: Institute of Microbiology, Swiss Federal Institute of Technology, ETH-Zentrum, CH-8092 Zürich, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Hummerjohann
authors.forename: S
authors.initials: S
authors.lastname: Laudenbach
authors.forename: J
authors.initials: J
authors.lastname: Rétey
authors.forename: T
authors.initials: T
authors.lastname: Leisinger
authors.forename: M A
authors.initials: MA
authors.lastname: Kertesz
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001192:Arylsulfatases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001692:Biological Transport / Q000235:genetics; D003001:Cloning, Molecular; D004952:Esters / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018085:Regulon / Q000235:genetics*; D013431:Sulfates / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism* / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; C062886:CysB protein, Bacteria; D004952:Esters; D013431:Sulfates; D015534:Trans-Activators; D013455:Sulfur; D001192:Arylsulfatases