[Spreading of porcine kidney epithelial cells under normal condition and under the effect of inhibitors of energy metabolism. I. Dynamics of spreading].
In the present work dynamics of cell spreading on a solid substratum has been investigated in normal conditions and under ATP-synthesis inhibitors. Sodium aside (20 mM), which blocks ATP-synthesis in mitochondria, and N,N-dicyclohexylcarbodiimide (DCCD) (100 mM), which blocks both ATP-synthesis and glycolysis, were ATP-synthesis inhibitors of choice. In the range from the moment of cell plating to 24 h three stages of cell spreading could be distinguished according to the dynamical change of the average projected cell area. At the first stage, within 1.5 h in the control culture the cell area increases rapidly. The slowing of spreading occurs at the second stage, within 1.5-4.0 h. The third stage is characterized by a slow but pronounced increase in cell spreading, which ceases in 24 h. Under inhibitory treatment, the pattern of cell spreading during the first 4 h is essentially the same as in control conditions. The subsequent DCCD action results in cell spreading inhibition; sodium aside, on the contrary, accelerates the spreading. The cell shape analysis has demonstrated that even as early as in 0.5 h the first small polarized cells appear simultaneously with the nonpolarized cells. In control cells, the share of polarized cells increases in almost 2 h, conversely, under drug actions the process of polarization begins earlier to be more pronounced in the presence of sodium aside. Thus, it has been shown that the spreading of PK cells does not require any additional ATP-synthesis. At early stages in normal conditions and under inhibitory treatment the picture of cell spreading is the same. A complete inhibition of ATP-synthesis slow down the process of cell spreading. However, an activation of these processes was observed in cells with low content of ATP, resulting from glycolysis retaining.
Kisurina-Evgen'eva
O P
OP
Department of Cytology and Histology, Biological Faculty, Moscow State University.
Onishchenko
G E
GE
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D004734:Energy Metabolism / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019810:Sodium Azide / Q000494:pharmacology*; D013552:Swine
D004024:Dicyclohexylcarbodiimide; D000255:Adenosine Triphosphate; D019810:Sodium Azide