title: Concentrations of leucocyte-associated chemotaxin C5a correlate with the mobilisation of polymorphonuclear leucocytes during operations for rectal cancer.
abstract: OBJECTIVE
  To describe the profile of potential chemotaxins C5a, interleukin 8 and tumour-necrosis factor alpha, and the peripheral blood leucocyte (PBL) response in five patients having uncomplicated operations for rectal cancer.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  University hospital, Norway.
  
  
  METHODS
  Five patients, four men and one woman, median age 66 years (range 48-77) who were operated on for rectal cancer.
  
  
  METHODS
  Four had low anterior resections and total mesorectal excision (TME) and one patient had a diverting end sigmoidostomy because of local perirectal spread of the cancer and liver metastases. Blood samples were taken at the start of the operation; peroperatively after 1, 2, and 3 hours; postoperatively at 5, 8, and 24 hours, for analysis of potential chemotaxins.
  
  
  RESULTS
  The number of PBL tripled between the start and end of the operation and declined to a slightly lower plateau between 5 and 24 hours. Peroperatively, the association of C5a with PBL increased six-fold resulting in a doubled concentration of C5a/PBL, whereas the corresponding concentration of C5a in plasma remained relatively constant. Postoperatively, the concentration of C5a associated with the PBL and in plasma fluctuated with the maximums being at 8 and 24 hours, respectively. In contrast to C5a in plasma, the concentration of cell-associated C5a correlated with number of PBL or polymorphonuclear leucocytes (PMN). The plasma concentration of IL-8 doubled during the operation, reached a six-fold maximum at 5 hours, and declined after 24 hours to twice the initial concentration. There were no correlations between plasma IL-8 and either number of PBL, plasma C5a, or PBL-associated C5a. No TNF alpha was detected in the plasma.
  
  
  CONCLUSIONS
  Tissue trauma caused by uncomplicated excision of rectal cancer leads to leucocyte mobilisation peroperatively, probably partly by complement activation and subsequent generation and binding of the chemotaxin C5a to PBL. However, other chemotaxins may also play a role.
authors.affiliation: Department of Gastroenterological Surgery, Ullevål Hospital, Oslo, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Johnson
authors.forename: H
authors.initials: H
authors.lastname: Langeggen
authors.forename: G
authors.initials: G
authors.lastname: Hetland
mesh_terms: D000368:Aged; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D015936:Complement C5a / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*; D011446:Prospective Studies; D012004:Rectal Neoplasms / Q000276:immunology* / Q000601:surgery*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D015936:Complement C5a