title: Autologous transplantation of ex vivo expanded bone marrow cells grown from small aliquots after high-dose chemotherapy for breast cancer.
abstract: The collection of small aliquots of bone marrow (BM), followed by ex vivo expansion for autologous transplantation may be less morbid, and more cost-effective, than typical BM or blood stem cell harvesting. Passive elimination of contaminating tumor cells during expansion could reduce reinoculation risks. Nineteen breast cancer patients underwent autotransplants exclusively using ex vivo expanded small aliquot BM cells (900-1200 x 10(6)). BM was expanded in media containing recombinant flt3 ligand, erythropoietin, and PIXY321, using stromal-based perfusion bioreactors for 12 days, and infused after high-dose chemotherapy. Correlations between cell dose and engraftment times were determined, and immunocytochemical tumor cell assays were performed before and after expansion. The median volume of BM expanded was 36.7 mL (range 15.8-87.0). Engraftment of neutrophils greater than 500/microL and platelets greater than 20,000/microL were 16 (13-24) and 24 (19-45) days, respectively; 1 patient had delayed platelet engraftment, even after infusion of back-up BM. Hematopoiesis is maintained at 24 months, despite posttransplant radiotherapy in 18 of the 19 patients. Transplanted CD34(+)/Lin(-) (lineage negative) cell dose correlated with neutrophil and platelet engraftment, with patients receiving greater than 2.0 x 10(5) CD34(+)/Lin(-) cells per kilogram, engrafting by day 28. Tumor cells were observed in 1 of the 19 patients before expansion, and in none of the 19 patients after expansion. It is feasible to perform autotransplants solely with BM cells grown ex vivo in perfusion bioreactors from a small aliquot. Engraftment times are similar to those of a typical 1000 to 1500 mL BM autotransplant. If verified, this procedure could reduce the risk of tumor cell reinoculation with autotransplants and may be valuable in settings in which small stem cell doses are available, eg, cord blood transplants. (Blood. 2000;95:2169-2174)
authors.affiliation: Department of Medicine, Loyola University Medical Center, Maywood, IL 60153, USA.
authors.forename: P
authors.initials: P
authors.lastname: Stiff
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Franklin
authors.forename: D
authors.initials: D
authors.lastname: Oldenberg
authors.forename: E
authors.initials: E
authors.lastname: Hsi
authors.forename: R
authors.initials: R
authors.lastname: Bayer
authors.forename: E
authors.initials: E
authors.lastname: Shpall
authors.forename: J
authors.initials: J
authors.lastname: Douville
authors.forename: R
authors.initials: R
authors.lastname: Mandalam
authors.forename: D
authors.initials: D
authors.lastname: Malhotra
authors.forename: T
authors.initials: T
authors.lastname: Muller
authors.forename: R D
authors.initials: RD
authors.lastname: Armstrong
authors.forename: A
authors.initials: A
authors.lastname: Smith
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000276:immunology; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D001943:Breast Neoplasms / Q000097:blood / Q000188:drug therapy / Q000628:therapy*; D016190:Carboplatin / Q000008:administration & dosage; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000276:immunology; D003520:Cyclophosphamide / Q000008:administration & dosage; D005260:Female; D006801:Humans; D008875:Middle Aged; D012008:Recurrence; D013852:Thiotepa / Q000008:administration & dosage; D013997:Time Factors; D019172:Transplantation Conditioning / Q000379:methods*; D014182:Transplantation, Autologous / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D018952:Antigens, CD34; D000970:Antineoplastic Agents; D003520:Cyclophosphamide; D013852:Thiotepa; D016190:Carboplatin