title: Helminthic transmission and isolation of Ehrlichia risticii, the causative agent of Potomac horse fever, by using trematode stages from freshwater stream snails.
abstract: We report successful helminthic transmission of Ehrlichia risticii, the causative agent of Potomac horse fever, using trematode stages collected from Juga yrekaensis snails. The ehrlichial agent was isolated from the blood of experimentally infected horses by culture in murine monocytic cells and identified as E. risticii ultrastructurally and by characterization of three different genes.
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616, USA. npusterla@ucdavis.edu
authors.forename: N
authors.initials: N
authors.lastname: Pusterla
authors.forename: J E
authors.initials: JE
authors.lastname: Madigan
authors.forename: J S
authors.initials: JS
authors.lastname: Chae
authors.forename: E
authors.initials: E
authors.lastname: DeRock
authors.forename: E
authors.initials: E
authors.lastname: Johnson
authors.forename: J B
authors.initials: JB
authors.lastname: Pusterla
mesh_terms: D000818:Animals; D004536:Ehrlichia / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D016873:Ehrlichiosis / Q000635:transmission / Q000662:veterinary*; D005260:Female; D005618:Fresh Water; D006376:Helminths / Q000382:microbiology*; D006734:Horse Diseases / Q000635:transmission*; D006736:Horses; D008264:Macrophages / Q000382:microbiology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009919:Orchiectomy; D016133:Polymerase Chain Reaction / Q000379:methods; D012908:Snails / Q000469:parasitology*; D014617:Vacuoles / Q000382:microbiology