Induction of experimental allergic encephalomyelitis in the NIH minipig.
Experimental allergic encephalomyelitis (EAE) was induced in the NIH minipig to create a large animal model of multiple sclerosis with a well-characterized immune system. Sixteen NIH minipigs were inoculated with minipig spinal cord homogenate (SCH). The clinical course was primarily monophasic, but re-induction was possible. CNS and blood lymphocytes specifically proliferated to SCH. Flow cytometry of CNS-isolated cells and SCH-stimulated PBMC revealed a shift to CD4(+) CD8(+) cells. Pathology demonstrated demyelination in the CNS white matter with perivascular mononuclear cell infiltrates of CD3(+)CD4(+)CD45(+) lymphocytes with a subset CD8(+). Pathology and in vitro SCH responses implicate a central role of CD4(+) lymphocytes in swine EAE.
Singer
B A
BA
Neuroimmunology Branch, National Institute of Neurological Disease and Stroke National Institutes of Health, 10 Center Drive, Bldg. 10, Rm. 5B16, Bethesda, MA 20892, USA. bnvsinger@earthlink.net
Tresser
N J
NJ
Frank
J A
JA
McFarland
H F
HF
Biddison
W E
WE
D000818:Animals; D001921:Brain / Q000276:immunology; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D009103:Multiple Sclerosis / Q000209:etiology; D013552:Swine; D013556:Swine, Miniature