Determination of N1-methylnicotinamide by HPLC postcolumn photoirradiation.
A method for the determination of N1-Methylnicotinamide (NMNA) was developed by the fluorescence formed by UV irradiation after separation by HPLC with a mobile phase containing hydrogen peroxide and 18-crown-6. This method was applied to human urine and serum.
Mawatari
K
K
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Iinuma
F
F
Watanabe
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D043844:Crown Ethers; D004988:Ethers, Cyclic; D006801:Humans; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D014466:Ultraviolet Rays
D043844:Crown Ethers; D004988:Ethers, Cyclic; D007202:Indicators and Reagents; D009536:Niacinamide; C015762:18-crown-6; D006861:Hydrogen Peroxide; C024058:N(1)-methylnicotinamide