title: [Changes in the virulence factor expression level in Listeria monocytogenes under various environmental conditions].
abstract: Effects of chelators Chelex-100 and activated charcoal on the production of proteins responsible for virulence of Listeria monocytogenes, facultative intracellular parasite were studied. Bivalent cation chelator Chelex-100 stimulates the production of only thiol-dependent hemolysin listeriolysin O. The presence of activated charcoal, a nonspecific chelator, in culture medium stimulated the expression of listeriolysin O and other main virulence factors by increasing the level of their transcription.
authors.forename: S A
authors.initials: SA
authors.lastname: Ermolaeva
authors.forename: Iu F
authors.initials: IuF
authors.lastname: Belyĭ
authors.forename: I S
authors.initials: IS
authors.lastname: Tartakovskiĭ
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins; D002606:Charcoal / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000378:metabolism; D006460:Hemolysin Proteins; D008074:Lipoproteins / Q000378:metabolism; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010740:Phospholipases / Q000378:metabolism; D012117:Resins, Synthetic; D010738:Type C Phospholipases / Q000378:metabolism; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002614:Chelating Agents; D003470:Culture Media; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D008074:Lipoproteins; C116063:P60 protein, bacteria; D012117:Resins, Synthetic; C024997:Chelex 100; D002606:Charcoal; D010740:Phospholipases; D010738:Type C Phospholipases; D043265:Phosphatidylinositol Diacylglycerol-Lyase; C038705:hlyA protein, Listeria monocytogenes