Adenylyl cyclase isoforms in the vestibular periphery of the rat.
The expression of adenylyl cyclase (AC) isoforms in the adult rat vestibular periphery was investigated using reverse transcription polymerase chain reaction (RT-PCR). AC II, IV and V mRNAs were expressed in both Scarpa's ganglion and vestibular end organs. In addition, in the vestibular end organs, an AC mRNA not previously reported in the rat was identified. The cloned sequence (GenBank accession no. AF184150) represented 95 amino acids with 100% similarity to the human AC VII and 94% to the bovine AC VII. AC VII mRNA also was found in the cerebellum but was undetectable in heart, kidney, liver and Scarpa's ganglion.
Wackym
P A
PA
Department of Otolaryngology, Medical College of Wisconsin, 9200 W. Wisconsin Avenue, Milwaukee, WI, USA. wackym@mcw.edu
Troyanovskaya
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M
Popper
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P
D000262:Adenylyl Cyclases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D017950:Ganglia, Sensory / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D014725:Vestibular Nerve / Q000166:cytology / Q000378:metabolism*; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000378:metabolism*
D020033:Protein Isoforms; D012333:RNA, Messenger; D000262:Adenylyl Cyclases