Ammonia concentration in bovine follicular fluid and its effect during in vitro maturation on subsequent embryo development.
10 mm) to determine ammonia concentration. Ammonia concentration was greater (P<0.001) in the bFF from smaller follicles compared with bFF from larger follicles, and ammonia concentration decreased (P10 mm follicles, respectively. In Experiment 2, abattoir oocytes (n=2885) were matured in vitro in media containing ammonia at the concentrations of 0 microM (Control), 29 microM (A1), 88 microM (A2), 132 microM (A3), 176 microM (A4) and 356 microM (A5), followed by in vitro fertilization and culture. There was no difference (P0.05) in cleavage rates and morula or blastocyst development between treatments, indicating that in vitro exposure of bovine oocytes to ammonia does not adversely influence subsequent embryonic development.
Hammon
D S
DS
Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 5600 Old Main Hill, Logan, UT 84332-5600, USA. hammon@cc.usu.edu
Wang
S
S
Holyoak
G R
GR
D000641:Ammonia / Q000009:adverse effects / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D015571:Follicular Fluid / Q000737:chemistry / Q000502:physiology*; D008297:Male; D009865:Oocytes / Q000254:growth & development; D011247:Pregnancy; D011897:Random Allocation
D000641:Ammonia