title: Use of calibrated viral load standards for group M subtypes of human immunodeficiency virus type 1 to assess the performance of viral RNA quantitation tests.
abstract: Optimal management of human immunodeficiency virus type 1 (HIV-1) disease requires accurate quantitation of viral RNA concentrations in plasma. Evidence for increasing geographic intermixing of HIV-1 subtypes makes equivalent quantitation of all subtypes essential. The performances of six quantitative viral RNA tests are described, for the first time, with calibrated viral isolates of diverse subtypes.
authors.affiliation: Henry M. Jackson Foundation, Walter Reed Army Institute of Research, Rockville, Maryland 20850, USA. liagodzinski@hiv.hjf.org
authors.forename: L L
authors.initials: LL
authors.lastname: Jagodzinski
authors.forename: D L
authors.initials: DL
authors.lastname: Wiggins
authors.forename: J L
authors.initials: JL
authors.lastname: McManis
authors.forename: S
authors.initials: S
authors.lastname: Emery
authors.forename: J
authors.initials: J
authors.lastname: Overbaugh
authors.forename: M
authors.initials: M
authors.lastname: Robb
authors.forename: S
authors.initials: S
authors.lastname: Bodrug
authors.forename: N L
authors.initials: NL
authors.lastname: Michael
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000175:diagnosis*; D002138:Calibration; D005843:Geography; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D019562:Viral Load / Q000379:methods*
chemical_list: D012367:RNA, Viral