Charcoal suspension for tumor labelling modifies macrophage activity in mice.
We have previously developed a charcoal suspension for injection into human breast cancers in order to facilitate their location during surgery. We observed that charcoal particles were ingested by intra and peritumoral macrophages, some of which carried the particles at some distance from the injection site. We studied the influence of the formulation parameters of the charcoal suspension for intratumoral injection on in vitro and in vivo activation and in vivo mobilization of mouse peritoneal macrophages after intra-peritoneal injection of 2 mL of each preparation. The influence of the charcoal origin (peat vs wood), granulometry, suspension vehicle (water for parenteral injection, vs saline), concentration and excipients were studied. Micronized peat charcoal in water for injection at the highest studied concentration reduced macrophage activation in vitro and in vivo. However, macrophage mobilization was weaker than after thioglycolate injection and did not seem to be charcoal dose-dependent. The additives incorporated in the charcoal suspension led in vivo to increased peritoneal macrophage activation and mobilization (mannitol, and glucose), only increased activation (polysorbate 80 and pluronic F68) or mobilization (dextran 40, egg lecithin, and cabosil), or inhibited both activation and mobilization (cremophor EL).
Bonhomme-Faivre
L
L
Laboratoire de Pharmacologie, Service Pharmacie, Hôpital Paul-Brousse, Villejuif, France. laurence.bonhomme-faivre@pbr.ap-hop-paris.fr
Depraetere
P
P
Savelli
M P
MP
Amdidouche
D
D
Bizi
E
E
Seiller
M
M
Orbach-Arbouys
S
S
D000818:Animals; D019540:Area Under Curve; D002606:Charcoal / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008163:Luminescent Measurements; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology*; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects; D013501:Surface-Active Agents / Q000494:pharmacology; D013535:Suspensions
D013501:Surface-Active Agents; D013535:Suspensions; D002606:Charcoal