title: Large scale, liquid phase oligonucleotide synthesis by alkyl H-phosphonate approach.
abstract: A new approach to the liquid phase synthesis of oligonucleotide is described, it is based on oxidative coupling using alkyl H-phosphonate synthon and polyethylene glycol (PEG5000) as a soluble support. Nucleoside alkyl H-phosphonate undergoes oxidative coupling in presence of NBS. The use of polyethylene glycol as a soluble polymeric support preserves some convenient features of the solid phase synthesis with new interesting advantages. This liquid phase method appears effective in terms of speed and coupling yield and can be evaluated for the production of large amount of oligonucleotide (100 microM).
authors.affiliation: Department of Chemistry, The Institute of Science, Mumbai, India.
authors.forename: K J
authors.initials: KJ
authors.lastname: Padiya
authors.forename: M M
authors.initials: MM
authors.lastname: Salunkhe
mesh_terms: D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D009841:Oligonucleotides; D063065:Organophosphonates