Protective effects of bergenin, the major constituent of Mallotus japonicus, on D-galactosamine-intoxicated rat hepatocytes.
This study was designed to investigate the effects of bergenin against D-galactosamine-induced injury in primary cultured rat hepatocytes. Bergenin (100 microM) decreased the release of glutamic pyruvic transaminase and sorbitol dehydrogenase by 62 and 50%, respectively, into hepatocyte medium incubated for 14 h with 1.5 mM galactosamine. Decreased RNA synthesis by 1.5 mM galactosamine was recovered 2.5 times compared with that of control hepatocytes at 100 microM bergenin. Therefore, the present results suggest that bergenin show hepatoprotective effects against galactosamine-intoxicated rat hepatocytes by inhibiting the release of glutamic pyruvic transaminase and sorbitol dehydrogenase as well as by increasing RNA synthesis.
Lim
H K
HK
Department of Pharmacology, College of Pharmacy, Chungbuk National University, Cheongju, South Korea.
Kim
H S
HS
Chung
M W
MW
Kim
Y C
YC
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002469:Cell Separation; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D005688:Galactosamine / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D010946:Plants, Medicinal / Q000737:chemistry*; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013385:Succinate Dehydrogenase / Q000097:blood
D001578:Benzopyrans; D012313:RNA; D005688:Galactosamine; D013385:Succinate Dehydrogenase; D000410:Alanine Transaminase; C006741:bergenin