Effects of Re-arterialization on early graft function and regeneration in the rat model of heterotopic auxiliary liver transplantation.
In the rat model of heterotopic auxiliary liver transplantation, graft re-arterialization may influence the outcome of inter-liver competition. This was investigated in the current study using two transplanted groups with or without graft re-arterialization. Immediately after reperfusion, the re-arterialized grafts showed significantly higher bile flow rate and bilirubin excretion than the grafts without re-arterialization. DNA synthesis rate was also increased more drastically in the re-arterialized group following the transplantation. Without re-arterialization, the rats developed more pronounced cytolysis and cholestasis. Among the long-term survivors, all healthy re-arterialized grafts regenerated, whereas 5/6 non-re-arterialized grafts atrophied. These data demonstrate that the re-arterialization increases graft survival by improving early hepatic function, enhancing regenerative response and preventing post-transplant biliary complications in this rat model.
Fan
Y D
YD
Division of Experimental Surgery, Department of Surgery, University Hospital of Ghent, Belgium. yedong.fan@rug.ac.be
Praet
M
M
Vanzieleghem
B
B
Vanwynsberghe
D
D
Stoop
D
D
Leroux-Roels
G
G
Delanghe
J
J
de Hemptinne
B
B
D000818:Animals; D001646:Bile / Q000502:physiology; D004247:DNA / Q000096:biosynthesis; D006085:Graft Survival; D006499:Hepatic Artery / Q000601:surgery*; D008099:Liver / Q000098:blood supply / Q000503:physiopathology; D008115:Liver Regeneration; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D016042:Transplantation, Heterotopic
D004247:DNA