title: Reactions of pentachlorophenol with laccase from Coriolus versicolor.
abstract: Laccase, purified from Coriolus versicolor, removed pentachlorophenol (PCP) from solution at pH 5, depending on initial PCP concentration and amount of laccase. With 100 units of laccase, 100% of 25 microg ml(-1) PCP and 60% of 200 microg ml(-1) PCP were removed respectively over 72 h. No free chloride was released in the reaction. In reaction with 100 microg PCP, products were primarily polymers (about 80,000 MW) with only 2-3 pg of o- and p-chloranils formed. Polymers were stable to acid hydrolysis and no release of PCP, or other low-molecular-weight products, was detected over several weeks. Laccase has a potential use in the biotreatment of aqueous effluents containing PCP, with polymerised products being removed from solution due to their high molecular weight.
authors.affiliation: Fungal Biotechnology Group, School of Biosciences, University of Westminster, London, UK.
authors.forename: M A
authors.initials: MA
authors.lastname: Ullah
authors.forename: C T
authors.initials: CT
authors.lastname: Bedford
authors.forename: C S
authors.initials: CS
authors.lastname: Evans
mesh_terms: D001673:Biodegradation, Environmental; D002850:Chromatography, Gel; D005658:Fungi / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D042845:Laccase; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D010416:Pentachlorophenol / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D013696:Temperature
chemical_list: D011108:Polymers; D010416:Pentachlorophenol; D010088:Oxidoreductases; D042845:Laccase