title: Primary HIV-1 infection of human CD4+ T cells passaged into SCID mice leads to selection of chronically infected cells through a massive fas-mediated autocrine suicide of uninfected cells.
abstract: We have recently shown that a human CD4+ T cell line (CEM-SS) acquires the permissiveness to M-tropic strains and primary isolates of HIV-1 after transplantation into SCID mice. This permissiveness was associated with the acquisition of a memory (CD45RO+) phenotype as well as of a functional CCR5 coreceptor. In this study, we have used this model for invest-igating in vivo the relationships between HIV-1 infection, apoptosis and T cell differentiation. When an in vivo HIV-1 infection was performed, the CEM cell tumors grew to a lower extent than the uninfected controls. CEM cells explanted from uninfected SCID mice (ex vivo CEM) underwent a significant level of spontaneous apoptosis and proved to be CD45RO+, Fas+ and Fas-L+, while Bcl-2 expression was significantly reduced as compared to the parental cells. Acute HIV-1 infection markedly increased apoptosis of uninfected ex vivo CEM cells, through a Fas/Fas-L-mediated autocrine suicide/fratricide, while parental cells did not undergo apoptosis following viral infection. The susceptibility to apoptosis of ex vivo CEM cells infected with the NSI strain of HIV-1, was progressively lost during culture, in parallel with the loss of Fas-L and marked changes in the Bcl-2 cellular distribution. On the whole, these results are strongly reminiscent of a series of events possibly occurring during HIV-1 infection. After an initial depletion of bystander CD4+ memory T cells during acute infection, latently or chronically infected CD4+ T lymphocytes are progressively selected and are protected against spontaneous apoptosis through the development of an efficient survival program. Studies with human cells passaged into SCID mice may offer new opportunities for an in vivo investigation of the mechanisms involved in HIV-1 infection and CD4+ T cell depletion.
authors.affiliation: Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Parlato
authors.forename: S M
authors.initials: SM
authors.lastname: Santini
authors.forename: C
authors.initials: C
authors.lastname: Lapenta
authors.forename: M
authors.initials: M
authors.lastname: Spada
authors.forename: M
authors.initials: M
authors.lastname: Logozzi
authors.forename: P
authors.initials: P
authors.lastname: Rizza
authors.forename: E
authors.initials: E
authors.lastname: Proietti
authors.forename: F
authors.initials: F
authors.lastname: Belardelli
authors.forename: S
authors.initials: S
authors.lastname: Fais
mesh_terms: D000818:Animals; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000637:transplantation / Q000821:virology*; D002460:Cell Line; D015658:HIV Infections / Q000473:pathology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009374:Neoplasms, Experimental / Q000503:physiopathology; D010641:Phenotype; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D019014:fas Receptor