MDP77: A novel neurite-outgrowth-promoting protein predominantly expressed in chick muscles.
A 4.7 kb chick cDNA clone that coded for the novel muscle-derived protein, MDP77, was isolated from a cDNA library of the denervated crus muscles using an antibody which inhibited the neurite outgrowth activity. MDP77 consisted of 676 aa with a calculated molecular mass of 77 k. The deduced amino acid sequence exhibited an extended coiled-coil domain and a leucine zipper motif. A recombinant protein promoted the neurite-outgrowth from the cultured chick neurons of the spinal cord in a dose-dependent manner. Northern blotting and in situ hybridization revealed that MDP77 was predominantly expressed in the cardiac and the skeletal muscles. In the COS-7 cells transfected with the cDNA of the epitope-tagged MDP77, the expressed protein was detected in the culture medium, suggesting that the MDP77 was secreted.
Uyeda
A
A
Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, 560-8531, Japan.
Fukui
I
I
Fujimori
K
K
Kiyosue
K
K
Nishimune
H
H
Kasai
M
M
Taguchi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002642:Chick Embryo; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D016501:Neurites; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
C406335:MDP77 protein, Gallus gallus; D009414:Nerve Growth Factors; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins