The effects of DNA containing CpG motif on dendritic cells.
Dendritic cells (DC) are specialized antigen-presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co-stimulatory signals to T cells. We describe an immunostimulatory oligonucleotide containing a CpG motif that stimulated murine DC to up-regulate co-stimulatory molecules, induce T-cell proliferative responses and secrete interleukin-12 in vitro. Administration of this oligonucleotide, but not of a control oligonucleotide lacking this motif, to mice led to the disappearance of DC from the marginal zone and T-cell areas of spleen, but not from heart or kidney. The same CpG did not cause maturation of monocyte-derived human DC in vitro, but lipopolysaccharide-treated monocyte-derived DC showed enhanced functional activity and up-regulated co-stimulatory molecules.
Behboudi
S
S
Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Oxford, UK.
Chao
D
D
Klenerman
P
P
Austyn
J
J
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D051940:B7-2 Antigen; D019013:CD40 Antigens / Q000032:analysis; D002465:Cell Movement; D002478:Cells, Cultured; D018899:CpG Islands; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016167:Integrin alphaXbeta2 / Q000276:immunology; D018664:Interleukin-12 / Q000096:biosynthesis*; D037181:Lectins, C-Type; D008070:Lipopolysaccharides / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens; D009841:Oligonucleotides / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000032:analysis; D013154:Spleen / Q000276:immunology
D015703:Antigens, CD; D051940:B7-2 Antigen; D019013:CD40 Antigens; C497964:CD86 protein, human; C497965:Cd86 protein, mouse; C093569:DEC-205 receptor; D000949:Histocompatibility Antigens Class II; D016167:Integrin alphaXbeta2; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D015778:Minor Histocompatibility Antigens; D009841:Oligonucleotides; D011956:Receptors, Cell Surface; D018664:Interleukin-12