title: [Changes to the hepatocytes and muscle fibers of the diaphragm during adaptation to cold under normal conditions and in combination with high-altitude hypoxia].
abstract: Comparative morphometric analysis of muscular fibres of the diaphragm and hepatocytes of albino rats was carried out in adaptation to cold (0-2 degrees C for 42 d) and to combined effect of cold and high-mountain hypoxia (2-5 degrees C temperature, 4000 m height, 40 d duration of the experiment). It was demonstrated that in adaptation to cold in normobaric conditions muscular fibres of the diaphragm develop moderate hypertrophy of mitochondria, accumulation of lipid inclusions with glycogen content remaining close control level. In combined effect of cold and high-mountain hypoxia direction of these changes was remained while their manifestation was increased. Relative volume of mitochondria was increased in hepatocytes in both experiments while volumetric indexes of rough endoplasmic reticulum and glycogen grew smaller. On one hand, the peculiarities of the dynamics of quantitative parameters of diaphragm muscular fibres and hepatocytes revealed in conditions of the long-term effect of the cold and in its combination with high mountain hypoxia indicate different efficiency of adaptive processes in the organ depending on the character of the effect and their organ-specificity on the other.
authors.affiliation: Laboratory of Electron Microscopy, Siberia Section, Russian Academy of Medical Sciences, Novosibirsk.
authors.forename: M D
authors.initials: MD
authors.lastname: Shmerling
authors.forename: E E
authors.initials: EE
authors.lastname: Filiushina
authors.forename: I I
authors.initials: II
authors.lastname: Buzueva
mesh_terms: D000222:Adaptation, Physiological; D000531:Altitude; D000818:Animals; D003080:Cold Temperature; D003964:Diaphragm / Q000503:physiopathology / Q000648:ultrastructure*; D000860:Hypoxia / Q000473:pathology* / Q000503:physiopathology; D008099:Liver / Q000503:physiopathology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D018485:Muscle Fibers, Skeletal / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D012800:Siberia; D013696:Temperature; D013997:Time Factors