title: Glycosylated major urinary protein of the house mouse: characterization of its N-linked oligosaccharides.
abstract: A minor component of the major urinary protein complex of the house mouse was chromatographically isolated and ascertained to be a previously suspected glycoprotein. Using highly sensitive mass-spectrometric techniques for sequencing and linkage analysis, the N-linked oligosaccharides of this glycoprotein were characterized. They were determined to be of the complex type with a wide heterogeneity. The heterogeneity was due to both the degree of sialylation and the presence of galactose residues in either beta(1-3) or beta(1-4) linkages. The biantennary structures were the most pronounced glycans, while tri- and tetraantennary entities were minor.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Mechref
authors.forename: L
authors.initials: L
authors.lastname: Zidek
authors.forename: W
authors.initials: W
authors.lastname: Ma
authors.forename: M V
authors.initials: MV
authors.lastname: Novotny
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry; D006031:Glycosylation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D011506:Proteins; C035737:major urinary proteins