Determination of barbiturates in urine by micellar liquid chromatography and direct injection of sample.
A liquid chromatographic procedure for the determination of six barbiturates (barbital, diallyl barbituric acid, phenobarbital, butabarbital, amobarbital and pentobarbital) in urine samples is described. The proposed system uses a Spherisorb octadecyl-silane ODS-2 C18 analytical column and a guard column of similar characteristics. The UV detector was set at 240 nm. A study to select adequate composition of the micellar mobile phase for the separation of these compounds in urine samples is performed. Maximum resolution was achieved with a 0.07 M sodium dodecylsulphate-0.3% propanol at pH 7.4 eluent. Limits of detection at 240 nm were ranged between 0.13 microg ml(-1) for diallyl barbituric acid and 2.7 microg ml(-1) for amobarbital. The procedure allows for the determination of these compounds in 20 minutes, it does not require prior a sample preparation step and it can be very useful to the investigation of intoxication.
Martín-Biosca
Y
Y
Departamento de Química Analítica, Universitat de València, Burjassot, Spain.
Sagrado
S
S
Villaneuva-Camañas
R M
RM
Medina-Hernández
M J
MJ
D000433:1-Propanol; D017738:Alkanesulfonic Acids; D000654:Amobarbital / Q000652:urine; D001462:Barbital / Q000652:urine; D001463:Barbiturates / Q000652:urine*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010424:Pentobarbital / Q000652:urine; D010634:Phenobarbital / Q000652:urine
D017738:Alkanesulfonic Acids; D001463:Barbiturates; C017273:dodecylsulfonic acid; D001462:Barbital; C084828:allobarbital; D000433:1-Propanol; D000654:Amobarbital; D010424:Pentobarbital; C084833:secbutabarbital; D010634:Phenobarbital