A simple method to evaluate the concentration of pentachlorophenol degraders in contaminated soils.
A new most probable number (MPN) method for the determination of pentachlorophenol (PCP) degraders in soil using the change in pH due to PCP degradation is compared with a well documented MPN method using radiolabeled PCP. The results of all MPN counts were similar within a 95% confidence limit. The results obtained in MPN per gram of dry soil using pH measurements were 1.8 (+3.1, -1.03) x10 (4) compared to 0.64 (+1.34, -0.42) x 10(4) when using production of [(14)C]CO(2).
Bécaert
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V
NSERC Industrial Chair for Site Remediation and Management, Department of Chemical Engineering, Ecole Polytechnique, P.O. Box 6079, Station Centre-ville, Montréal, Que., Canada.
Deschênes
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L
Samson
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R
D001419:Bacteria / Q000254:growth & development* / Q000378:metabolism*; D001431:Bacteriological Techniques; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D015169:Colony Count, Microbial; D006863:Hydrogen-Ion Concentration; D010416:Pentachlorophenol / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
D002250:Carbon Radioisotopes; D012989:Soil Pollutants; D002245:Carbon Dioxide; D010416:Pentachlorophenol