Correlation of TGF beta 1 overexpression with down-regulation of proliferation-inducing molecules in HPV-11 transformed human tissue xenografts.
The epidemiologic association of human papillomavirus (HPV) infection with dysplasia and cervical cancer is well established. Transforming growth factor beta 1 (TGF beta 1) has regulatory effects on a broad spectrum of cell types and is a growth inhibitory protein for epithelial cells. To examine the phenotype of experimentally generated, HPV-11 transformed human tissues, we looked at expression of TGF beta 1 and a number of proliferation-enhancing molecules which are known to be regulated by TGF beta 1, including bcl-2, c-myc, c-Ha-ras, c-jun and NFkB. HPV-11 transformed xenografts showed up-regulation of TGF beta 1 expression and down-regulation of the expression levels of bcl-2, c-myc, c-Ha-ras, c-jun and NFkB. These results suggest that TGF beta 1 may exert antiproliferative effects on HPV-11 transformed papillomas by down-regulating different proliferation-enhancing molecules.
Shier
M K
MK
Department of Microbiology and Immunology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine 17033, USA.
Neely
E B
EB
Ward
M G
MG
Richards
M E
ME
Manders
E C
EC
Meyers
C
C
Howett
M K
MK
D001483:Base Sequence; D002455:Cell Division / Q000502:physiology*; D002472:Cell Transformation, Viral; D017931:DNA Primers; D015536:Down-Regulation; D006801:Humans; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D027383:Papillomaviridae / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000502:physiology*; D015854:Up-Regulation
D017931:DNA Primers; D016328:NF-kappa B; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta