title: A 6-Mb high-resolution physical and transcription map encompassing the hereditary prostate cancer 1 (HPC1) region.
abstract: Several hereditary disease loci have been genetically mapped to the chromosome 1q24-q31 interval, including the hereditary prostate cancer 1 (HPC1) locus. Here, we report the construction of a 20-Mb yeast artificial chromosome contig and a high-resolution 6-Mb sequence-ready bacterial artificial chromosome (BAC)/P1-derived artificial chromosome (PAC) contig of 1q25 by sequence and computational analysis, STS content mapping, and chromosome walking. One hundred thirty-six new STSs, including 10 novel simple sequence repeat polymorphisms that are being used for genetic refinement of multiple disease loci, have been generated from this contig and are shown to map to the 1q25 interval. The integrity of the 6-Mb BAC/PAC contig has been confirmed by restriction fingerprinting, and this contig is being used as a template for human chromosome 1 genome sequencing. A transcription mapping effort has resulted in the precise localization of 18 known genes and 31 ESTs by database searching, exon trapping, direct cDNA hybridization, and sample sequencing of BACs from the 1q25 contig. An additional 11 known genes and ESTs have been placed within the larger 1q24-q31 interval. These transcription units represent candidate genes for multiple hereditary diseases, including HPC1.
authors.affiliation: Cancer Genetics Branch, Bethesda, Maryland 20892, USA. jdc@nhgri.nih.gov
authors.forename: J D
authors.initials: JD
authors.lastname: Carpten
authors.forename: I
authors.initials: I
authors.lastname: Makalowska
authors.forename: C M
authors.initials: CM
authors.lastname: Robbins
authors.forename: N
authors.initials: N
authors.lastname: Scott
authors.forename: R
authors.initials: R
authors.lastname: Sood
authors.forename: T D
authors.initials: TD
authors.lastname: Connors
authors.forename: T I
authors.initials: TI
authors.lastname: Bonner
authors.forename: J R
authors.initials: JR
authors.lastname: Smith
authors.forename: M U
authors.initials: MU
authors.lastname: Faruque
authors.forename: D A
authors.initials: DA
authors.lastname: Stephan
authors.forename: H
authors.initials: H
authors.lastname: Pinkett
authors.forename: S D
authors.initials: SD
authors.lastname: Morgenbesser
authors.forename: K
authors.initials: K
authors.lastname: Su
authors.forename: C
authors.initials: C
authors.lastname: Graham
authors.forename: S G
authors.initials: SG
authors.lastname: Gregory
authors.forename: H
authors.initials: H
authors.lastname: Williams
authors.forename: L
authors.initials: L
authors.lastname: McDonald
authors.forename: A D
authors.initials: AD
authors.lastname: Baxevanis
authors.forename: K W
authors.initials: KW
authors.lastname: Klingler
authors.forename: G M
authors.initials: GM
authors.lastname: Landes
authors.forename: J M
authors.initials: JM
authors.lastname: Trent
mesh_terms: D001483:Base Sequence; D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1; D020451:Contig Mapping; D016172:DNA Fingerprinting / Q000379:methods; D018076:DNA, Complementary; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary