A simple drying method for field emission scanning electron microscopy for chromosomes.
Hexamethyldisilazane treatment and subsequent air drying of spread plant chromosomes is compared with critical point drying. The two procedures are equivalent for preparing chromosomes for examination by field emission scanning electron microscopy at low voltage.
Bock
C
C
Max-Planck Institute for Behavioral Physiology Seewiesen, Starnberg, Germany. Bock@mpi-seewiesen.mpg.de
Duschanek
I
I
Martin
R
R
D002875:Chromosomes / Q000648:ultrastructure*; D003890:Desiccation; D005404:Fixatives; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D017646:Organosilicon Compounds; D012434:Secale; D014908:Triticum
D005404:Fixatives; D017646:Organosilicon Compounds; C024548:hexamethylsilazane