Construction of HIV Rev peptides containing peptide nucleic acid that bind HIV RRE IIB RNA.
Peptides containing peptide nucleic acid (PNA) have been designed and synthesized to construct molecules recognizing a bulge or a loop structure of RNA. Such peptides were here designed from the HIV Rev protein that can bind the stem-loop IIB of the Rev responsive element (RRE) RNA. Variations of PNA modulated the binding affinities of the peptides to RRE IIB RNA.
Kumagai
I
I
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohoma, Japan.
Takahashi
T
T
Hamasaki
K
K
Ueno
A
A
Mihara
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002942:Circular Dichroism; D015195:Drug Design; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D015694:Gene Products, rev / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics*; D015752:Genes, env / Q000235:genetics*; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D017433:Protein Structure, Secondary / Q000187:drug effects; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000138:chemical synthesis / Q000737:chemistry; D012235:Rhodamines; D014764:Viral Proteins / Q000138:chemical synthesis / Q000235:genetics; D054321:rev Gene Products, Human Immunodeficiency Virus
D005456:Fluorescent Dyes; D015694:Gene Products, rev; D020135:Peptide Nucleic Acids; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012235:Rhodamines; D014764:Viral Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D001120:Arginine