title: Proteins in the early golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p.
abstract: The yeast tSNARE Sed5p is considered to mainly reside in the early Golgi compartment at the steady state of its intracellular cycling. To better understand this compartment, we immunoisolated a membrane subfraction having Sed5p on the surface (the Sed5 vesicles). Immunoblot studies showed that considerable portions (20-30%) of the Golgi mannosyltransferases (Mnt1p, Van1p, and Mnn9p) were simultaneously recovered while the late Golgi (Kex2p) or endoplasmic reticulum (Sec71p) proteins were almost excluded. The N-terminal sequences of the polypeptides detectable by Coomassie blue staining indicated that the prominent components of the Sed5 vesicles include Anp1p, Emp24p, Erv25p, Erp1p, Ypt52p, and a putative membrane protein of unknown function (Yml067c).
authors.affiliation: Department of Biotechnology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
authors.forename: J H
authors.initials: JH
authors.lastname: Cho
authors.forename: Y
authors.initials: Y
authors.lastname: Noda
authors.forename: K
authors.initials: K
authors.lastname: Yoda
mesh_terms: D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D015151:Immunoblotting; D008364:Mannosyltransferases / Q000032:analysis / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D050765:Qa-SNARE Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins; D020539:Sequence Analysis, Protein; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins / Q000032:analysis / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins
chemical_list: D002352:Carrier Proteins; C093262:EMP24 protein, S cerevisiae; C102179:ERV25 protein, S cerevisiae; D005656:Fungal Proteins; D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; C077554:Sed5 protein, S cerevisiae; D033921:Vesicular Transport Proteins; C090211:ANP1 protein, S cerevisiae; D008364:Mannosyltransferases; C086867:YPT52 protein, S cerevisiae; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins