title: Clink, a nanovirus-encoded protein, binds both pRB and SKP1.
abstract: Clink, a 20-kDa protein of faba bean necrotic yellows virus, a single-stranded DNA plant virus, interacts with pRB family members and a SKP1 homologue from Medicago sativa. An LxCxE motif and an F-box of Clink mediate the interactions with the respective proteins. The capacity of Clink to bind pRB correlates with its ability to stimulate viral replication. Interaction of a single protein with the cell cycle regulator pRB and SKP1, a constituent of the ubiquitin-protein turnover pathway, appears to be a novel feature. Hence, Clink may represent a new class of viral cell cycle modulators.
authors.affiliation: Institut des Sciences Végétales, CNRS, 91198 Gif sur Yvette, France.
authors.forename: M N
authors.initials: MN
authors.lastname: Aronson
authors.forename: A D
authors.initials: AD
authors.lastname: Meyer
authors.forename: J
authors.initials: J
authors.lastname: Györgyey
authors.forename: L
authors.initials: L
authors.lastname: Katul
authors.forename: H J
authors.initials: HJ
authors.lastname: Vetten
authors.forename: B
authors.initials: B
authors.lastname: Gronenborn
authors.forename: T
authors.initials: T
authors.lastname: Timchenko
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D016160:Retinoblastoma Protein / Q000378:metabolism*; D044786:S-Phase Kinase-Associated Proteins; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C406062:Clink protein, faba bean necrotic yellows virus; D004279:DNA, Viral; D016160:Retinoblastoma Protein; D044786:S-Phase Kinase-Associated Proteins; D014764:Viral Proteins