title: Antigenic variation of Borrelia turicatae Vsp surface lipoproteins occurs in vitro and generates novel serotypes.
abstract: As a means of avoiding the host immune response, the tick-borne relapsing fever spirochete Borrelia turicatae undergoes antigenic variation in its abundant surface lipoproteins. In this study, B. turicatae strain Oz1, serotype B, was subcultured in vitro and cloned by limited dilutions after 50 passages. Four different serotypes (serotypes A, B, E, and F) differing by their expressed Vsp lipoproteins were isolated. Using pulsed-field gel electrophoresis, we showed that the variability in surface-exposed proteins is correlated with rearrangement between different linear plasmids, defining serotype-specific plasmid profiles. Moreover, we determined the nucleotide sequence of genes encoding the VspE and VspF lipoproteins, corresponding to the two novel serotypes E and F, respectively. Our results showed that antigenic variation in B. turicatae occurs spontaneously in vitro, in the absence of immune selection.
authors.affiliation: Unité de bactériologie moléculaire et médicale, Institut Pasteur, Paris, France.
authors.forename: N M
authors.initials: NM
authors.lastname: Ras
authors.forename: D
authors.initials: D
authors.lastname: Postic
authors.forename: P
authors.initials: P
authors.lastname: Ave
authors.forename: M
authors.initials: M
authors.lastname: Huerre
authors.forename: G
authors.initials: G
authors.lastname: Baranton
mesh_terms: D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D015139:Blotting, Southern; D001898:Borrelia / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008074:Lipoproteins; C121437:Vsp protein, bacterial