title: Clustering of clinical strains of Helicobacter pylori analyzed by two-dimensional gel electrophoresis.
abstract: Strain variations of Helicobacter pylori have been tested by numerous methods and compared among different patient groups. The aim of this study was to investigate whether H. pylori expresses disease-specific proteins that can be detected by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). H. pylori strains isolated from duodenal ulcer, gastric cancer, and gastritis patients were analyzed. Extensive variation in spot patterns was observed between the strains, but a dendrogram analysis revealed that some strains within each disease group clustered together. Eight proteins were sequenced and found in the H. pylori genome sequence. 2-D PAGE is a useful method for studies of protein expression and for highlighting the extensive strain variation that H. pylori exhibits.
authors.affiliation: Department of Medical Epidemiology, Karolinska Institute, Stockholm, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Enroth
authors.forename: T
authors.initials: T
authors.lastname: Akerlund
authors.forename: A
authors.initials: A
authors.lastname: Sillén
authors.forename: L
authors.initials: L
authors.lastname: Engstrand
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D004381:Duodenal Ulcer / Q000382:microbiology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005756:Gastritis / Q000382:microbiology; D016480:Helicobacter pylori / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D017421:Sequence Analysis; D013274:Stomach Neoplasms / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins