title: Molecular characterization of Cryptosporidium isolates obtained from human immunodeficiency virus-infected individuals living in Switzerland, Kenya, and the United States.
abstract: A total of 22 Cryptosporidium isolates from human immunodeficiency virus-infected patients from Kenya, Switzerland, and the United States were examined at three genetic loci: the 18S ribosomal DNA, HSP-70, and acetyl coenzyme A synthetase genes. Four distinct Cryptosporidium genotypes were identified: (i) the Cryptosporidium parvum "human" genotype, (ii) the C. parvum "cattle" genotype, (iii) Cryptosporidium felis, and (iv) Cryptosporidium meleagridis. This is the first report of C. meleagridis in a human host. These results and those of others indicate that immunocompromised individuals are susceptible to a wide range of Cryptosporidium species and genotypes. Future studies are required to understand the full public health significance of Cryptosporidium genotypes and species in immunocompromised hosts.
authors.affiliation: World Health Organisation Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and State Agricultural Biotechnology Centre, Murdoch University, Murdoch, WA 6150, Australia. morgan@numbat.murdoch.edu.au
authors.forename: U
authors.initials: U
authors.lastname: Morgan
authors.forename: R
authors.initials: R
authors.lastname: Weber
authors.forename: L
authors.initials: L
authors.lastname: Xiao
authors.forename: I
authors.initials: I
authors.lastname: Sulaiman
authors.forename: R C
authors.initials: RC
authors.lastname: Thompson
authors.forename: W
authors.initials: W
authors.lastname: Ndiritu
authors.forename: A
authors.initials: A
authors.lastname: Lal
authors.forename: A
authors.initials: A
authors.lastname: Moore
authors.forename: P
authors.initials: P
authors.lastname: Deplazes
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis* / Q000469:parasitology; D000106:Acetate-CoA Ligase / Q000235:genetics; D000818:Animals; D003457:Cryptosporidiosis / Q000175:diagnosis*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D015658:HIV Infections / Q000150:complications / Q000635:transmission; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018451:Homosexuality, Male; D006801:Humans; D007630:Kenya; D008297:Male; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D015819:Substance Abuse, Intravenous; D013557:Switzerland; D014481:United States
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D018840:HSP70 Heat-Shock Proteins; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D000106:Acetate-CoA Ligase