The interaction between O2 and CO2 exchange in rainbow trout during graded sustained exercise.
A quantitative analysis of O2 and CO2 transport was conducted in resting and exercising rainbow trout, and these data were used to quantify the magnitude of coupling between O2 and CO2 exchange, in vivo. The release of Bohr protons during haemoglobin-oxygenation was non-linear over the Hb-O2 equilibrium curve used in trout subjected to different levels of sustained exercise. At low swimming speeds, when venous blood O2 content (CvO2) was high, there was a small acidosis as blood passed through the gills, indicating more protons were released during oxygenation of Hb than were consumed during HCO3- dehydration. At higher swimming speeds, when CvO2 was low, there was a significant alkalosis in arterial relative to venous blood, indicating that fewer protons were released upon oxygenation than HCO3- ions were dehydrated to CO2. Haldane coefficients (moles of protons released per mole of O2 which binds to Hb), calculated from steady state arterial and mixed-venous parameters, revealed that under resting conditions all blood CO2 removed from the blood during gill transit was stoichiometrically related to O2 uptake through the release of Bohr protons during Hb oxygenation. The magnitude of coupling between CO2 excretion and O2 uptake decreased from 100% to less than 40% at the maximal swimming velocity when the largest region of the Hb-O2 equilibrium curve was used for gas exchange. The non-linear release of Bohr protons over the range of Hb-O2 saturation in the blood reduces HCO3- dehydration at the gills during greater work loads elevating arterial P(CO2) levels, leading to an increase in HCO3- buffer capacity of the blood and tissues.
Brauner
C J
CJ
Department of Zoology, University of British Columbia, Vancouver, Canada. braunerc@mcmaster.ca
Thorarensen
H
H
Gallaugher
P
P
Farrell
A P
AP
Randall
D J
DJ
D000818:Animals; D001692:Biological Transport; D002245:Carbon Dioxide / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D009043:Motor Activity / Q000502:physiology*; D017686:Oncorhynchus mykiss / Q000097:blood / Q000502:physiology*; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption; D010108:Oxyhemoglobins / Q000378:metabolism; D011659:Pulmonary Gas Exchange / Q000502:physiology*; D013550:Swimming
D010108:Oxyhemoglobins; D002245:Carbon Dioxide; D010100:Oxygen