title: The effect of acute immobilization stress on the abundance of corticotropin-releasing factor receptor in lymphoid organs.
abstract: We have previously found a dramatic increase of corticotropin-releasing factor receptor (CRF-R1) production in splenic neutrophils of male C57BL/6N mice after application of an immunological stimulus. We demonstrate here that immobilization, a predominantly psychological stress, exhibited a similar effect. Shortly after 90 min of immobilization, the number of splenic CRF-RI+ cells was transiently increased by nearly 8-fold, while it was reduced in thymus and unchanged in lymph nodes. The CRF-R1+ cells were detected by an affinity-purified polyclonal antibody directed against the N-terminus of CRF-R1, and identified as neutrophils, eosinophils or their immature precursors on the basis of their nuclear shapes, Wright-Giemsa staining and colocalization of CRF-R1 with the ER-MP58 antigen.
authors.affiliation: Department of Molecular Neuroendocrinology, Max Planck Institute for Experimental Medicine, Goettingen, Germany. mradulovic@mail.mpiem.gwdg.de
authors.forename: M
authors.initials: M
authors.lastname: Radulovic
authors.forename: C
authors.initials: C
authors.lastname: Weber
authors.forename: J
authors.initials: J
authors.lastname: Spiess
mesh_terms: D000818:Animals; D002452:Cell Count; D004804:Eosinophils / Q000166:cytology / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D007103:Immobilization / Q000502:physiology; D008198:Lymph Nodes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013312:Stress, Physiological / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D000099052:CRF Receptor, Type 1
chemical_list: D018019:Receptors, Corticotropin-Releasing Hormone; D000099052:CRF Receptor, Type 1