title: Synthesis of N-substituted 4-(4-hydroxyphenyl)piperidines, 4-(4-hydroxybenzyl)piperidines, and (+/-)-3-(4-hydroxyphenyl)pyrrolidines: selective antagonists at the 1A/2B NMDA receptor subtype.
abstract: Antagonists at the 1A/2B subtype of the NMDA receptor (NR1A/2B) are typically small molecules that consist of a 4-benzyl- or a 4-phenylpiperidine with an omega-phenylalkyl substituent on the heterocyclic nitrogen. Many of these antagonists, for example ifenprodil (1), incorporate a 4-hydroxy substituent on the omega-phenyl group. In this study, the position of this 4-hydroxy substituent was transferred from the omega-phenyl group to the benzyl or phenyl group located on the 4-position of the piperidine ring. Analogues incorporating pyrrolidine in lieu of piperidine were also prepared. Electrical recordings using cloned receptors expressed in Xenopus oocytes show that high-potency antagonists at the NR1A/2B subtype are obtained employing N-(omega-phenylalkyl)-substituted 4-(4-hydroxyphenyl)piperidine, 4-(4-hydroxybenzyl)piperidine, and (+/-)-3-(4-hydroxyphenyl)pyrrolidine as exemplified by 21 (IC(50) = 0.022 microM), 33 (IC(50) = 0.059 microM), and 40 (IC(50) = 0.017 microM), respectively. These high-potency antagonists are >1000 times more potent at the NR1A/2B subtype than at either the NR1A/2A or NR1A/2C subtypes. The binding affinities of 21 at alpha(1)-adrenergic receptors ([(3)H]prazosin, IC(50) = 0.54 microM) and dopamine D2 receptors ([(3)H]raclopride, IC(50) = 1.2 microM) are reduced by incorporating a hydroxy group onto the 4-position of the piperidine ring and the beta-carbon of the N-alkyl spacer to give (+/-)-27: IC(50) NR1A/2B, 0.026; alpha(1), 14; D2, 105 microM. The high-potency phenolic antagonist 21 and its low-potency O-methylated analogue 18 are both potent anticonvulsants in a mouse maximal electroshock-induced seizure (MES) study (ED(50) (iv) = 0.23 and 0.56 mg/kg, respectively). These data indicate that such compounds penetrate the blood-brain barrier but their MES activity may not be related to NMDA receptor antagonism.
authors.affiliation: CoCensys, Inc., 213 Technology Drive, Irvine, California 92618, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Guzikowski
authors.forename: A P
authors.initials: AP
authors.lastname: Tamiz
authors.forename: M
authors.initials: M
authors.lastname: Acosta-Burruel
authors.forename: S
authors.initials: S
authors.lastname: Hong-Bae
authors.forename: S X
authors.initials: SX
authors.lastname: Cai
authors.forename: J E
authors.initials: JE
authors.lastname: Hawkinson
authors.forename: J F
authors.initials: JF
authors.lastname: Keana
authors.forename: S R
authors.initials: SR
authors.lastname: Kesten
authors.forename: C T
authors.initials: CT
authors.lastname: Shipp
authors.forename: M
authors.initials: M
authors.lastname: Tran
authors.forename: E R
authors.initials: ER
authors.lastname: Whittemore
authors.forename: R M
authors.initials: RM
authors.lastname: Woodward
authors.forename: J L
authors.initials: JL
authors.lastname: Wright
authors.forename: Z L
authors.initials: ZL
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D004597:Electroshock; D018691:Excitatory Amino Acid Antagonists / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009865:Oocytes / Q000502:physiology; D010880:Piperidines / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011759:Pyrrolidines / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats; D017207:Rats, Sprague-Dawley; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism; D011954:Receptors, Dopamine / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors*; D012640:Seizures / Q000188:drug therapy / Q000209:etiology; D014982:Xenopus laevis
chemical_list: D000927:Anticonvulsants; D018691:Excitatory Amino Acid Antagonists; D010880:Piperidines; D011759:Pyrrolidines; D018340:Receptors, Adrenergic, alpha-1; D011954:Receptors, Dopamine; D016194:Receptors, N-Methyl-D-Aspartate