Intensity modulated HSQC and HMQC: two simple methods to measure 3J(HNH)alpha in proteins.
Two methods for the measurement of homonuclear 3J(HNH)alpha coupling constants are described. Both HSQC- and HMQC-type experiments employ 'quantitative J-correlation', in which the coupling constant of interest is obtained from the intensity ratio of cross peaks of two spectra. The first spectrum is acquired with 3J(HNH)alpha evolution and the second with alpha-proton decoupling. The resolution of these methods in the F1-domain is not restricted.
Permi
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P
Institute of Biotechnology, University of Helsinki, Finland.
Kilpeläinen
I
I
Annila
A
A
Heikkinen
S
S
D000577:Amides / Q000737:chemistry*; D000596:Amino Acids / Q000737:chemistry*; D008962:Models, Theoretical; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D011522:Protons
D000577:Amides; D000596:Amino Acids; D011506:Proteins; D011522:Protons