title: Suppression of chimpanzee NORS in hamster/chimpanzee hybrid: report on cell line R48-26.
abstract: We present the first documented NOR suppression in a hybridoma other than man-mouse for the hamster-chimpanzee hybrid cell line R48-26. Alu PCR and chromosome painting showed that in this cell line chimpanzee chromosomes 13-15-23 are maintained. NORs on chimpanzee chromosomes 15-23, whose presence was directly verified by FISH with H 28s rDNA, resulted inactive while telomeric rDNA on hamster chromosomes resulted active even if hamster chromosomes presented extensive rearrangements. We observed an all or nothing model in accordance with a model of regulation by selective transcriptional factors. The rearrangements of hamster chromosomes have not involved the location of NORs because they maintain a telomeric position.
authors.affiliation: Istituto di Antropologia Università di Firenze.
authors.forename: P
authors.initials: P
authors.lastname: Cavagna
authors.forename: R
authors.initials: R
authors.lastname: Marzella
authors.forename: M
authors.initials: M
authors.lastname: Rocchi
authors.forename: B
authors.initials: B
authors.lastname: Chiarelli
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D020223:Chromosome Painting; D006224:Cricetinae; D003412:Cricetulus / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006822:Hybrid Cells / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D009697:Nucleolus Organizer Region / Q000235:genetics*; D002679:Pan troglodytes / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D016615:Telomere / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S