Spectral light quality affects protein profile of Synechococcus sp. PCC 7942: a comparative 2-dimensional gel electrophoresis (2-DGE) analysis.
We report here a comparative analysis of the effect of blue (450 nm), red (660 nm), and white light (400-700 nm) on the protein profile of cyanobacteria Synechococcus sp. PCC 7942. In vivo labeling of cells with [(35)S] methionine and their subsequent analysis by two-dimensional gel electrophoresis (2-DGE) showed that eight polypeptides were unique to dark adapted cells, ten were blue light specific, and four were specifically induced in red light. The results show that Synechococcus sp. respond to various light treatments rapidly and synthesize new polypeptides in dark and blue/red light.
Pandey
R
R
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Chauhan
S
S
Singhal
G S
GS
Kale
R K
RK
D001426:Bacterial Proteins / Q000378:metabolism*; D000458:Cyanobacteria / Q000254:growth & development / Q000378:metabolism*; D003624:Darkness; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008027:Light; D008715:Methionine / Q000378:metabolism; D013462:Sulfur Radioisotopes / Q000378:metabolism
D001426:Bacterial Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine