Role of activator protein-1 in TCR-mediated regulation of the murine fasl promoter.
The present study demonstrates that transcription factor interactions are important in regulating the murine fasl promoter following TCR-mediated activation. We used DNase I-footprinting, EMSAs, and transient transfection assays to identify the minimal TCR signal-responsive region within the fasl promoter. This region contains the previously identified binding sites for NF-kappaB and Egr and the AP-1 site identified in this study. We found that TCR signaling induces AP-1 binding to this site and regulates the fasl promoter function in a fashion dependent on NF-kappaB binding. However, mutation in the AP-1 site alone did not show a significant effect on the promoter function. The data suggest that the minimal promoter required at least two transcription factors to function.
Matsui
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Department of Medicine, Arthritis Center, Boston University School of Medicine, Boston, MA 02118, USA.
Xiao
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D000818:Animals; D018983:DNA Footprinting; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D003850:Deoxyribonuclease I; D004357:Drug Synergism; D051767:Early Growth Response Protein 2; D004591:Electrophoresis, Polyacrylamide Gel; D053222:Fas Ligand Protein; D005786:Gene Expression Regulation / Q000276:immunology*; D017874:Immediate-Early Proteins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009154:Mutation; D016328:NF-kappa B / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000276:immunology*; D011485:Protein Binding / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D017384:Sequence Deletion; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D017874:Immediate-Early Proteins; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D011948:Receptors, Antigen, T-Cell; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D003850:Deoxyribonuclease I