PLGA microspheres phagocytosis by pig alveolar macrophages: influence of poly(vinyl alcohol) concentration, nature of loaded-protein and copolymer nature.
The study aimed to investigate on a pig alveolar macrophage culture model the influence of: (1) poly(D,L-lactide-co-glycolide) characteristics, (2) the residual poly(vinyl alcohol) (PVA) and (3) the nature of encapsulated proteins, immunoglobulin Y (IgY) or bovine serum albumin, on the microspheres phagocytosis efficiency. The phagocytosis evaluation was performed by flow cytometry and has allowed a screening of microspheres formulations. The hydrophilicity of microspheres resulting from the nature of the polymer and/ or from the residual hydrophilic surface agent (PVA) led to a decrease of phagocytosis intensity. The phagocytosis results of IgY-loaded microspheres strongly suggested that the phagocytosis was increased when the phagocytic cell possessed a receptor for this protein on its surface.
Torche
A M
AM
Laboratoire de Pharmacie Galénique, Biopharmacie et Pharmacie Clinique, Université de Rennes, France. galenic@univ-rennes1.fr
Le Corre
P
P
Albina
E
E
Jestin
A
A
Le Verge
R
R
D000818:Animals; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000008:administration & dosage; D019344:Lactic Acid / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008863:Microspheres; D010587:Phagocytosis; D011100:Polyglycolic Acid / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013552:Swine
C016512:IgY; D007136:Immunoglobulins; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D011142:Polyvinyl Alcohol