title: Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects.
abstract: OBJECTIVE
  To determine whether changes in subcutaneous adipose tissue plasminogen activator inhibitor-1 (PAI-1) expression influence plasma PAI-1 level during weight loss in obese humans.
  
  
  METHODS
  Study of the variations of PAI-1 levels both in plasma and in subcutaneous abdominal adipose tissue in 15 volunteer non-diabetic obese subjects, body mass index (BMI) 40.4.+/-1.9 kg/m2, aged 48+/-3 y, before and after a 3 week very low calorie diet (VLCD) programme (3.9+/-0.1 MJ/day).
  
  
  METHODS
  Plasma and adipose tissue PAI-1 protein levels were measured by enzyme-linked immunosorbent assay and PAI-1 mRNA levels were quantified by quantitative RT-competitive PCR.
  
  
  RESULTS
  VLCD induced weight loss (5.8+/-0.8 kg) and decreased plasma PAI-1 concentration (-26% (P<0. 01)). Surprisingly, PAI-1 mRNA and protein abundance in subcutaneous adipose tissue increased by 87% (P<0.05) and by 44% (P<0.01), respectively.
  
  
  CONCLUSIONS
  These data indicate thus that changes in subcutaneous adipose tissue PAI-1 expression are not involved in the decrease of plasma PAI-1 levels during VLCD in obese subjects. International Journal of Obesity (2000)24, 70-74
authors.affiliation: Service de Biochimie, Hôpital de la Salpêtrière, AP-HP, Paris. jean-philippe.bastard@psl.ap-hop-paris.fr
authors.forename: J P
authors.initials: JP
authors.lastname: Bastard
authors.forename: H
authors.initials: H
authors.lastname: Vidal
authors.forename: C
authors.initials: C
authors.lastname: Jardel
authors.forename: E
authors.initials: E
authors.lastname: Bruckert
authors.forename: D
authors.initials: D
authors.lastname: Robin
authors.forename: P
authors.initials: P
authors.lastname: Vallier
authors.forename: P
authors.initials: P
authors.lastname: Blondy
authors.forename: G
authors.initials: G
authors.lastname: Turpin
authors.forename: C
authors.initials: C
authors.lastname: Forest
authors.forename: B
authors.initials: B
authors.lastname: Hainque
mesh_terms: D000005:Abdomen; D000273:Adipose Tissue / Q000378:metabolism*; D004038:Diet, Reducing; D002149:Energy Intake / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy / Q000235:genetics / Q000378:metabolism*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000378:metabolism; D015431:Weight Loss / Q000502:physiology
chemical_list: D017395:Plasminogen Activator Inhibitor 1