title: Triple helix assembly and processing of human collagen produced in transgenic tobacco plants.
abstract: The use of tobacco plants as a novel expression system for the production of human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding the human proalpha1(I) chain to generate transgenic tobacco plants expressing collagen I. The recombinant proalpha1(I) chains were expressed as disulfide-bonded trimers and were shown to fold into a stable homotrimeric triple helix. Moreover, the recombinant procollagen was subsequently processed to collagen as it occurs in animals. Large amounts of recombinant collagen were purified from field grown plant material. The data suggest that plants are a valuable alternative for the recombinant production of collagen for various medical and scientific purposes.
authors.affiliation: Institut de Biologie et Chimie des Protéines, CNRS UPR 412, Université Lyon I, 7 passage du Vercors, F-69367, Lyon, France.
authors.forename: F
authors.initials: F
authors.lastname: Ruggiero
authors.forename: J Y
authors.initials: JY
authors.lastname: Exposito
authors.forename: P
authors.initials: P
authors.lastname: Bournat
authors.forename: V
authors.initials: V
authors.lastname: Gruber
authors.forename: S
authors.initials: S
authors.lastname: Perret
authors.forename: J
authors.initials: J
authors.lastname: Comte
authors.forename: B
authors.initials: B
authors.lastname: Olagnier
authors.forename: R
authors.initials: R
authors.lastname: Garrone
authors.forename: M
authors.initials: M
authors.lastname: Theisen
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011347:Procollagen / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000648:ultrastructure; D014026:Nicotiana / Q000235:genetics*; D014357:Trypsin
chemical_list: D011347:Procollagen; D011994:Recombinant Proteins; D014357:Trypsin