title: Adoptive transfer of infectious bronchitis virus primed alphabeta T cells bearing CD8 antigen protects chicks from acute infection.
abstract: Infectious bronchitis virus (IBV) infection and associated illness may be dramatically modified by passive transfer of immune T lymphocytes. Lymphocytes collected 10 days postinfection were transferred to naive chicks before challenge with virus. As determined by respiratory illness and viral load, transfer of syngeneic immune T lymphocytes protected chicks from challenge infection, whereas no protection was observed in the chicks receiving the MHC compatible lymphocytes from uninfected chicks. Protection following administration of T lymphocytes could be observed in chicks with three distinct MHC haplotypes: B(8)/B(8), B(12)/B(12), and B(19)/B(19). Nearly complete elimination of viral infection and illness was observed in chicks receiving cells enriched in alphabeta lymphocytes. In contrast, removal of gammadelta T lymphocytes had only a small effect on their potential to protect chicks. The adoptive transfer of enriched CD8(+) or CD4(+) T lymphocytes indicated that protection was also a function primarily of CD8-bearing cells. These results indicated that alphabeta T lymphocytes bearing CD8(+) antigens are critical in protecting chicks from IBV infection.
authors.affiliation: Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Seo
authors.forename: J
authors.initials: J
authors.lastname: Pei
authors.forename: W E
authors.initials: WE
authors.lastname: Briles
authors.forename: J
authors.initials: J
authors.lastname: Dzielawa
authors.forename: E W
authors.initials: EW
authors.lastname: Collisson
mesh_terms: D000208:Acute Disease; D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology / Q000821:virology*; D018352:Coronavirus Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006239:Haplotypes / Q000235:genetics; D001351:Infectious bronchitis virus / Q000276:immunology* / Q000502:physiology; D007668:Kidney / Q000821:virology; D008168:Lung / Q000276:immunology / Q000503:physiopathology / Q000821:virology; D008285:Major Histocompatibility Complex / Q000235:genetics / Q000276:immunology; D011828:Radiation Chimera; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology* / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology / Q000378:metabolism; D019562:Viral Load
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta