title: Programmed cell death in the myocardium of arrhythmogenic right ventricular cardiomyopathy in children and adults.
abstract: Arrhythmogenic right ventricular cardiomyopathy (ARVC) is characterized by fibrofatty replacement of the right ventricular myocardium. Recently, the myocardial loss in ARVC has been suggested to be related to apoptosis. However, it is still unknown whether this phenomenon is already established in the myocardium of pediatric cases with this disease. We examined the histopathologic characteristics of the ventricular myocardium in specimens obtained from 10 patients, including 3 children with ARVC, and investigated the occurrence of apoptosis in the myocardium by terminal deoxyribonucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay and agarose-gel electrophoresis of DNA. Endomyocardial biopsy specimens from the 10 cases and a necropsy sample from one adult case with ARVC were examined. Histopathologic examination of biopsy specimens from the pediatric cases revealed extensive fibrosis. Typical fatty infiltration was demonstrated in one of the 3 pediatric cases. These findings were similar to those in adult cases; the histopathologic index based on the severity of myocardial damage, including myocyte degeneration and fibrosis, was not significantly different from that in adult cases. TUNEL assay revealed positive reactivity of the myocardial cells. The apoptotic index was 1.4 +/- 0.4% in children and 1.6 +/- 0.5% in adults (difference not statistically significant). Agarose-gel electrophoresis of a DNA extract of the myocardial tissue of the autopsy case revealed DNA fragmentation. Cases with idiopathic ventricular tachycardia and control cases with a cardiac transplant (with no rejection) had minimal histopathologic findings and negative reactivity in the TUNEL assay. These results indicate that myocardial damage is already established in cases diagnosed as ARVC in childhood, and suggest that the myocardial damage is closely related to apoptosis in children, as well as in adults, in this disease.
authors.affiliation: Department of Pathology, Tokyo Women's Medical University, Shinjuku-ku, Japan. nisikawa@clabo.zengaku.twmu.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Nishikawa
authors.forename: S
authors.initials: S
authors.lastname: Ishiyama
authors.forename: M
authors.initials: M
authors.lastname: Nagata
authors.forename: Y
authors.initials: Y
authors.lastname: Sakomura
authors.forename: M
authors.initials: M
authors.lastname: Nakazawa
authors.forename: K
authors.initials: K
authors.lastname: Momma
authors.forename: M
authors.initials: M
authors.lastname: Hiroe
authors.forename: T
authors.initials: T
authors.lastname: Kasajima
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D019571:Arrhythmogenic Right Ventricular Dysplasia / Q000473:pathology*; D001706:Biopsy; D002648:Child; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation; D004587:Electrophoresis, Agar Gel; D004699:Endocardium / Q000473:pathology; D005260:Female; D006352:Heart Ventricles / Q000473:pathology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*
chemical_list: D004247:DNA