title: Simultaneous stereoselective high-performance liquid chromatographic determination of 10-hydroxycarbazepine and its metabolite carbamazepine-10,11-trans-dihydrodiol in human urine.
abstract: An enantioselective HPLC method for the simultaneous determination of the concentration of the enantiomers of the oxcarbazepine metabolites 10-hydroxycarbazepine (MHD) and carbamazepine-10,11-trans-dihydrodiol (DHD) in human urine is described. The method is based on extraction with tert.-butylmethyl ether-dichloromethane (2:1, v/v) under alkaline conditions, separation and evaporation of the organic phase and dissolution of the residue in the mobile phase. Enantiomers are resolved on a Diacel Chiralcel OD column (250 mm x 4.6 mm I.D.) under isocratic conditions using as mobile phase n-hexane-ethanol-2-propanol (18:2:1, v/v/v) with addition of glacial acetic acid (0.1%). The enantiomers are detected by UV at 215 nm. The method allows reliable determination of the MHD and DHD enantiomers in human urine with limits of quantification of 0.2 mg/l and 0.4 mg/l, respectively.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, The Hebrew University of Jerusalem, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Volosov
authors.forename: M
authors.initials: M
authors.lastname: Bialer
authors.forename: S
authors.initials: S
authors.lastname: Xiaodong
authors.forename: E
authors.initials: E
authors.lastname: Perucca
authors.forename: A
authors.initials: A
authors.lastname: Sintov
authors.forename: B
authors.initials: B
authors.lastname: Yagen
mesh_terms: D002220:Carbamazepine / Q000031:analogs & derivatives* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: C039775:10,11-dihydro-10-hydroxycarbamazepine; C407264:carbamazepine-10,11-dihydrodiol; D002220:Carbamazepine