Simultaneous determination of paracetamol and chlorpheniramine in human plasma by liquid chromatography-tandem mass spectrometry.
An analytical method for the determination of paracetamol and chlorpheniramine in human plasma has been developed, validated and applied to the analysis of samples from a phase I clinical trial. The analytical method consists in the extraction of paracetamol and chlorpheniramine with diethyl ether, followed by the determination of both drugs by an LC-MS-MS method, using 2-acetamidophenol as internal standard. The intra-assay and inter-assay precision and accuracy of this technique were good and the limit of quantitation was 0.5 microg/ml of plasma for paracetamol and 0.2 ng/ml for chlorpheniramine. The concentration working range was established between 0.5 microg/ml and 25 microg/ml for paracetamol and between 0.2 ng/ml and 50 ng/ml for chlorpheniramine. This method has been used for analyzing more than 1200 human plasma samples from a clinical study with 24 volunteers.
Celma
C
C
Metabolism and Pharmacokinetics Department, Ipsen Pharma, Sant Feliu de Llobregat (Barcelona), Spain. carles.celma@beaufour-ipsen.com
Allué
J A
JA
Pruñonosa
J
J
Peraire
C
C
Obach
R
R
D000082:Acetaminophen / Q000097:blood*; D002744:Chlorpheniramine / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000082:Acetaminophen; D002744:Chlorpheniramine