title: Characterization of a zebrafish/mouse somatic cell hybrid panel.
abstract: We have characterized a collection of zebrafish/mouse somatic cell hybrids with 211 genes and markers chosen from the 25 zebrafish linkage groups. Most of the zebrafish genome is represented in this collection with 88% of genes/markers present in at least one hybrid cell line. Although most hybrids contain chromosomal fragments, there are a few instances where a complete or nearly complete zebrafish chromosome has been maintained in a mouse background, based on multiple markers covering the entire chromosome. In addition to their use in mapping studies, this collection of somatic cell hybrids should constitute an important tool as a source of specific chromosome fragments and for assessing the function of genome regions.
authors.affiliation: Urology Division, Department of Surgery, McGill University, Montreal, Quebec, H3G 1A4, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Chevrette
authors.forename: L
authors.initials: L
authors.lastname: Joly
authors.forename: P
authors.initials: P
authors.lastname: Tellis
authors.forename: E W
authors.initials: EW
authors.lastname: Knapik
authors.forename: J
authors.initials: J
authors.lastname: Miles
authors.forename: M
authors.initials: M
authors.lastname: Fishman
authors.forename: M
authors.initials: M
authors.lastname: Ekker
mesh_terms: D000818:Animals; D002460:Cell Line; D008040:Genetic Linkage; D005819:Genetic Markers; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D015027:Zebrafish / Q000235:genetics*
chemical_list: D005819:Genetic Markers