HLA-DR and HLA-DQ gene typing of infertile women possessing sperm-immobilizing antibody.
Thirty-eight infertile women, possessing sperm-immobilizing antibody (SIA), were examined for their HLA-DR and -DQ types using DNA obtained from peripheral blood cells. The typing of HLA-DR and DQ was performed by polymerase chain reaction sequence specific oligonucleotide probes (PCR-SSOP) and PCR-restriction fragment length polymorphism (RFLP), respectively. In comparison to the normal Japanese population, the SIA positive patient population had higher genes frequencies in HLA-DRB1*0901 (26.3 vs. 13.6%, P<0.005), DQB1*0602 (13.2 vs. 6.2%, P<0.05) and -DQB1*0303 (26.3 vs. 14.8%, P<0.01), but not in any HLA-DQA1 gene types by chi2 test. After Bonferroni correction, the high frequency of HLA-DRB1*0901 remained significant (P<0.05) and HLA-DQB1*303 was slightly significant (P<0.07) but no other genes had a gene frequency significantly higher than that of the normal Japanese population. HLA-DRB1*0901 and HLA-DQB1*0303 are very rare among Caucasians but characteristically high among Japanese. The high frequency of HLA-DRB1*0901 and DQB1*0303 genes in the Japanese population may account for higher frequency of sperm-immobilizing antibody in Japanese compared to other ethnic groups.
Tsuji
Y
Y
Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya, Japan. yoshi416@hyo-med.ac.jp
Mitsuo
M
M
Yasunami
R
R
Sakata
K
K
Shibahara
H
H
Koyama
K
K
D000918:Antibody Specificity; D044466:Asian People / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005802:Genes, MHC Class II; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D007247:Infertility, Female / Q000235:genetics / Q000276:immunology*; D007518:Isoantibodies / Q000032:analysis*; D007564:Japan; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013081:Sperm Motility / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D007518:Isoantibodies