title: Functional correlation of GABA(A) receptor alpha subunits expression with the properties of IPSCs in the developing thalamus.
abstract: GABA(A) receptor alpha1 and alpha2 subunits are expressed differentially with ontogenic period in the brain, but their functional roles are not known. We have recorded GABA(A) receptor-mediated IPSCs from laterodorsal (LD) thalamic relay neurons in slices of rat brain at various postnatal ages and found that decay times of evoked IPSCs and spontaneous miniature IPSCs undergo progressive shortening during the first postnatal month. With a similar time course, expression of transcripts and proteins of GABA(A) receptor alpha2 subunit in LD thalamic region declined, being replaced by those of alpha1 subunit. To further address the causal relationship between alpha subunits and IPSC decay time kinetics, we have overexpressed GABA(A) receptor alpha1 subunit together with green fluorescent protein in LD thalamic neurons in organotypic culture using recombinant Sindbis virus vectors. Miniature IPSCs recorded from the LD thalamic neurons overexpressed with alpha1 subunit had significantly faster decay time compared with control expressed with beta-galactosidase. We conclude that the alpha2-to-alpha1 subunit switch underlies the developmental speeding in the decay time of GABAergic IPSCs.
authors.affiliation: Department of Neurophysiology, University of Tokyo Faculty of Medicine, Tokyo 113-0033, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: K
authors.initials: K
authors.lastname: Onodera
authors.forename: C
authors.initials: C
authors.lastname: Van Renterghem
authors.forename: W
authors.initials: W
authors.lastname: Sieghart
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005822:Genetic Vectors; D015640:Ion Channel Gating / Q000502:physiology; D007700:Kinetics; D020647:Lateral Thalamic Nuclei / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000502:physiology*; D009474:Neurons / Q000737:chemistry / Q000502:physiology; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000502:physiology; D011963:Receptors, GABA-A / Q000737:chemistry / Q000235:genetics*; D012845:Sindbis Virus / Q000235:genetics; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011963:Receptors, GABA-A; D013779:Tetrodotoxin