Plasmid elimination and immunomodulation by 3-benzazepines in vitro.
For studying the mechanisms of biological activity on 3-benzazepines, antimicrobial effect, F'lac plasmid elimination activity (a plasmid curing effect on F'lac plasmid) and antibody-dependent cellular cytotoxicity (ADCC) test were performed. A weak antiplasmid effect was found at sub-inhibitory concentrations. A combination of [KF4] with verapamil [2] did not alter the ineffectivity, however, [KF4] could inhibit the antiplasmid effect of promethazine, as compared to the control (promethazine alone) plasmid curing effect. A competition between promethazine and [KF4] might exist in plasmid elimination effect. ADCC activity of human leukocytes was enhanced by KF1, KF2, KF3, DA and NE at 1.0 microgram/mL concentrations. The majority of 3-benzazepines [KS02, KM57, KN50, KE04, KI10, KP80] was ineffective for plasmid curing, however, inhibited the ADCC reaction, but they did not show a real dose-dependent effect.
Motohashi
N
N
Meiji Pharmaceutical University, Tokyo, Japan.
Kawase
M
M
Saito
S
S
Miskolci
C
C
Berek
L
L
Molnár
J
J
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology; D000920:Antibody-Dependent Cell Cytotoxicity; D001552:Benzazepines / Q000494:pharmacology*; D006801:Humans; D010957:Plasmids; D014700:Verapamil / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000890:Anti-Infective Agents; D001552:Benzazepines; D014700:Verapamil