title: Molecular and immunological analyses of the Borrelia turicatae Bdr protein family.
abstract: Here, we describe the molecular and immunological characterization of the bdr gene family of Borrelia turicatae, a relapsing-fever spirochete. Nine bdr alleles belonging to two different subfamilies were sequenced and localized to linear plasmids. Anti-Bdr antiserum was generated and used to analyze Bdr expression in pre- and postinfection isogenic populations. The analyses presented here provide a detailed characterization of the Bdr proteins in a relapsing-fever spirochete species, enhancing our understanding of these proteins at the genus-wide level.
authors.affiliation: Department of Microbiology, Medical College of Virginia at Virginia Commonwealth University, Richmond, Virginia 23298, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Carlyon
authors.forename: D M
authors.initials: DM
authors.lastname: Roberts
authors.forename: M
authors.initials: M
authors.lastname: Theisen
authors.forename: C
authors.initials: C
authors.lastname: Sadler
authors.forename: R T
authors.initials: RT
authors.lastname: Marconi
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry*; D001898:Borrelia / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D019143:Evolution, Molecular; D015723:Gene Library; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D010957:Plasmids; D017386:Sequence Homology, Amino Acid; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D015345:Oligonucleotide Probes