Activation of neurospecific gene expression by antennapedia homeobox peptide.
Antennapedia homeobox peptide has been reported to enhance neurite outgrowth and branching. Thus it is of interest to investigate whether antennapedia peptide is capable of modulating the expression of genes related to different events of neuronal development. In this paper we report the enhancement of a 68 KDa neurofilament subunit, choline acetyltransferase and acetylcholinesterase expression in spinal cord neurons, elicited by antennapedia peptide. Modulation of gene expression is different with respect to each gene product analyzed, suggesting a specific action of the peptide on diverse genes controlling different events of neuronal differentiation.
Biagioni
S
S
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma "La Sapienza", Piazzale A. Moro, 5, 00185, Roma, Italy.
Ciuffini
L
L
De Jaco
A
A
Vignoli
A L
AL
Augusti-Tocco
G
G
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D051943:Antennapedia Homeodomain Protein; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D002642:Chick Embryo; D002795:Choline O-Acetyltransferase / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018398:Homeodomain Proteins / Q000032:analysis / Q000235:genetics*; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology; D016501:Neurites / Q000737:chemistry / Q000201:enzymology*; D016900:Neurofilament Proteins / Q000032:analysis / Q000235:genetics; D009474:Neurons / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure*; D009687:Nuclear Proteins; D012333:RNA, Messenger / Q000032:analysis; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics; D015533:Transcriptional Activation / Q000502:physiology*
D051943:Antennapedia Homeodomain Protein; D018398:Homeodomain Proteins; D016900:Neurofilament Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C066858:neurofilament protein NF 68; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase