Simultaneous determination of 5-hydroxyindoles and catecholamines by HPLC with fluorometric precolumn derivatization.
We have developed a highly sensitive and simple precolumn derivatization method for simultaneous determination of 5-hydroxyindoles (5HIs) and catecholamines (CAs) with 4-dimethylaminobenzylamine (DMBA). The method was successfully applied to the determination of 5HIs and CAs in plasma and urine.
Iizuka
H
H
School of Pharmaceutical Sciences, Toho University, Chiba, Japan. iizuka@phar.toho-u.ac.jp
Ishige
T
T
Ohta
Y
Y
Yajima
T
T
D015127:9,10-Dimethyl-1,2-benzanthracene; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007211:Indoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D002395:Catecholamines; D007211:Indoles; C026785:5-hydroxyindole; D015127:9,10-Dimethyl-1,2-benzanthracene