Conductimetric membrane strip immunosensor with polyaniline-bound gold colloids as signal generator.
For point-of-care examination, an immuno-chromatographic assay system based on conductimetric detection was investigated by utilizing, as signal generator, colloidal gold with polyaniline bound on the metal surface. Although the gold is a widely used label for antibodies to produce colorimetric signals, the tracer does not lend itself for a suitable electric conduction along the gold particles due to the presence of protein barriers (e.g. immunoglobulin and blocking agent) against electron transfer. To overcome this problem, we introduced a conducting polymer, for instance, polyaniline, as a conductivity-modulating agent on the gold surface after immobilizing an antibody specific to human albumin used as model analyte. This novel signal generator amplified the conductimetric signal 4.7 times compared with the plain gold, and the signal was also maximum 2.3-fold higher than that from the photometric system under the same analytical conditions. The latter effect resulted from an exponential pattern in the dose-response curve of the electric signal that was different from the conventional sigmoidal shape.
Kim
J H
JH
Graduate School of Biotechnology, Korea University, Seoul, South Korea.
Cho
J H
JH
Cha
G S
GS
Lee
C W
CW
Kim
H B
HB
Paek
S H
SH
D000814:Aniline Compounds; D015374:Biosensing Techniques; D002845:Chromatography; D004553:Electric Conductivity; D006046:Gold; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*
D000814:Aniline Compounds; D006046:Gold; C023650:aniline