Ezrin links syndecan-2 to the cytoskeleton.
The syndecan family of heparan sulfate proteoglycans is known to associate with the actin cytoskeleton, possibly transducing signals from the extracellular matrix. In the search for proteins that could mediate the association of syndecan-2 with the actin cytoskeleton we found that ezrin, a protein which links membrane receptors to the cytoskeleton, coimmunoprecipitated with syndecan-2 in COS-1 cells. In vitro assays indicated a direct association between the amino-terminal domain of ezrin and the cytoplasmic domain of syndecan-2. Confocal microscopy showed colocalization of ezrin and syndecan-2 in actin-rich microspikes in COS-1 cells. The syndecan-2/ezrin protein complex was resistant to 0.2% Triton X-100 extraction but the syndecan-2/amino-terminal domain of ezrin complex was not, which indicated that carboxi-terminal domain of ezrin is involved in the cytoskeleton anchorage of this protein complex. Additionally we observed that the activation of rhoA GTPase increased syndecan-2 insolubility in 0.2% Triton X-100 and syndecan-2/ezrin association. Taken together, these results indicate that ezrin connects syndecan-2 to the actin cytoskeleton.
Granés
F
F
Department of Cellular Biology, Faculty of Biology, University of Barcelona, Avenue Diagonal 645, Spain.
Urena
J M
JM
Rocamora
N
N
Vilaró
S
S
D000818:Animals; D019556:COS Cells; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000378:metabolism / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D018613:Microscopy, Confocal; D010446:Peptide Fragments / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism / Q000502:physiology*; D010957:Plasmids / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011509:Proteoglycans / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D053669:Syndecan-2; D014162:Transfection; D020742:rhoA GTP-Binding Protein / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D011509:Proteoglycans; D053669:Syndecan-2; D020742:rhoA GTP-Binding Protein; D000099149:Ezrin