Simultaneous determination of rifampicin, isoniazid and pyrazinamide in tablet preparations by multivariate spectrophotometric calibration.
The use of multivariate spectrophotometric calibration is presented for the simultaneous determination of the active components of tablets used in the treatment of pulmonary tuberculosis. The resolution of ternary mixtures of rifampicin, isoniazid and pyrazinamide has been accomplished by using partial least squares (PLS-1) regression analysis. Although the components show an important degree of spectral overlap, they have been simultaneously determined with high accuracy and precision, rapidly and with no need of nonaqueous solvents for dissolving the samples. No interference has been observed from the tablet excipients. A comparison is presented with the related multivariate method of classical least squares (CLS) analysis, which is shown to yield less reliable results due to the severe spectral overlap among the studied compounds. This is highlighted in the case of isoniazid, due to the small absorbances measured for this component.
Goicoechea
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HC
Departamento de Química, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Paraje El Pozo, Santa Fe, Argentina.
Olivieri
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AC
D000995:Antitubercular Agents / Q000032:analysis* / Q000737:chemistry; D007538:Isoniazid / Q000032:analysis* / Q000737:chemistry; D015999:Multivariate Analysis; D011718:Pyrazinamide / Q000032:analysis* / Q000737:chemistry; D012293:Rifampin / Q000032:analysis* / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets
D000995:Antitubercular Agents; D013607:Tablets; D011718:Pyrazinamide; D007538:Isoniazid; D012293:Rifampin