[Examining interaction of phages with microorganisms by fluorometry and electro-orientation spectroscopy].
Bacterial sensitivity to different various phages was examined by electro-orientation spectroscopy, fluorometry, and electron microscopy. The strains of Pseudomonas aeruginosa, Staphylococcus aureus, Yersinia pestis, Mycobacterium smegmatis, and Xanthomonas campestris were used. The fluorescence intensity of a membranotropic agent in the ANS-cell-phage system was shown to depend on the interaction of a bacterial virus and a microorganism. Fluorometric data correlated with electro-orientation spectroscopic findings. An analysis of the low-frequency site makes it possible to determine phage adsorption on the bacterial surface. The changes in electro-orientation effects at high frequencies suggest that there are barrier dysfunctions in the external membranes and that there is cellular phage reproductions. Whether fluorometry and electro-orientation spectroscopy can be further used for rapid identification of microorganisms by using phages is discussed.
Zhilenkov
E L
EL
Fomchenkov
V M
VM
Novikov
I A
IA
Sadomov
V E
VE
Oborotov
M V
MV
Gremiakova
T A
TA
D001435:Bacteriophages / Q000502:physiology* / Q000648:ultrastructure; D005470:Fluorometry; D008242:Lysogeny / Q000502:physiology; D008561:Membrane Fusion; D009161:Mycobacterium / Q000502:physiology* / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000502:physiology* / Q000648:ultrastructure; D013057:Spectrum Analysis; D013211:Staphylococcus aureus / Q000502:physiology* / Q000648:ultrastructure; D016959:Xanthomonas campestris / Q000502:physiology* / Q000648:ultrastructure; D015010:Yersinia pestis / Q000502:physiology* / Q000648:ultrastructure