Cytoskeletal organization and incorporation of beta 3 integrin in thrombin-stimulated platelets: effect of acetylsalicylic acid.
Platelet stimulation by agonists is followed by changes in cytoskeletal organization that includes actin polymerization and association of the membrane skeleton (which is connected with the integrin alpha IIb beta 3) with the underlying cytoplasmic actin filaments. The effect of orally administered acetylsalicylic acid to healthy volunteers on incorporation of contractile protein and beta 3 integrin into the cytoskeletal core of thrombin-stimulated platelets was studied. Stimulation was followed by increased contractile protein and beta 3 incorporation into the cytoskeleton. Acetylsalicylic acid intake resulted in decreased incorporation of myosin and actin (32% and 20%, respectively), and a decrease (36%) in the association of beta 3 integrin with the cytoskeletal elements was evident. In conclusion, we have shown that acetylsalicylic acid, besides the known inhibitory effect on thromboxane synthesis, promotes changes in the cytoskeletal organization of thrombin-stimulated platelets that could limit thrombus formation.
Maeda
N Y
NY
Fundação Pró-Sangue Hemocentro de São Paulo, Brazil.
Lopes
A A
AA
Bydlowski
S P
SP
D000185:Actinin / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000187:drug effects / Q000378:metabolism; D015703:Antigens, CD / Q000187:drug effects* / Q000378:metabolism*; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology; D001792:Blood Platelets / Q000737:chemistry; D001797:Blood Protein Electrophoresis; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000187:drug effects / Q000378:metabolism; D003599:Cytoskeleton / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D039661:Integrin beta3; D016023:Integrins / Q000187:drug effects / Q000378:metabolism; D009218:Myosins / Q000187:drug effects / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects*; D010980:Platelet Membrane Glycoproteins / Q000187:drug effects* / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology*
D000199:Actins; D015703:Antigens, CD; D003598:Cytoskeletal Proteins; D039661:Integrin beta3; D016023:Integrins; D010980:Platelet Membrane Glycoproteins; D000185:Actinin; D017830:Octoxynol; D013917:Thrombin; D009218:Myosins; D001241:Aspirin