Water and monovalent ions in the minor groove of B-DNA oligonucleotides as seen by NMR.
During the past 8 years, two complementary nmr techniques-magnetic relaxation dispersion and nuclear Overhauser effect spectroscopy-have been applied extensively to the study of water and monovalent ions in the minor groove of B-DNA oligonucleotides in solution. In this review, the possibilities and limitations of the two methods are outlined, with emphasis on the interpretational steps whereby molecular-level information is extracted from the primary data. The results on sequence-dependent hydration and ion-DNA interactions obtained so far by these methods is summarized and critically assessed. The nmr results are also compared with structural data from x-ray crystallography.
Halle
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B
Physical Chemistry 2, Lund University, P. O. Box 124, S-22100 Lund, Sweden. bertil.halle@fkem2.lth.se
Denisov
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VP
D001483:Base Sequence; D002414:Cations, Monovalent / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000648:ultrastructure; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000737:chemistry* / Q000378:metabolism
D002414:Cations, Monovalent; D009838:Oligodeoxyribonucleotides; D014867:Water; D004247:DNA