title: Bcl-2 and Bax proteins are nuclear matrix associated proteins.
abstract: Bcl-2 and Bax proteins are implicated in the regulation of apoptosis. Nuclear matrix has been demonstrated to be associated with a vast array of functional and regulatory properties of cells. NuMA is one member of a class of nuclear matrix proteins that resides in both the nucleus and mitotic apparatus. The nuclear lamins appear to form a thin fibrous structure immediately underlying the inner nuclear membrane of eukaryotic cell nuclei. The association of bcl-2 and Bax protein with nuclear matrix in glioblastoma cell line U343 was studied by confocal microscopy and Western blotting. Confocal microscopic images display that bcl-2 was localized at the peripheral of the nuclear matrix and Bax protein was located in the nuclear matrix. Western blotting detected a 26 kDa bcl-2 band and a specific band of Bax at around 66 kDa in nuclear matrix proteins. Our results suggest that bcl-2 and Bax proteins are nuclear matrix associated proteins.
authors.affiliation: Department of Anatomy, Chinese University of Hong Kong, Shatin, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Wang
authors.forename: M X
authors.initials: MX
authors.lastname: Ding
authors.forename: S B
authors.initials: SB
authors.lastname: Chew-Cheng
authors.forename: J P
authors.initials: JP
authors.lastname: Yun
authors.forename: E C
authors.initials: EC
authors.lastname: Chew
mesh_terms: D034961:Antigens, Nuclear; D015153:Blotting, Western; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D018613:Microscopy, Confocal; D009687:Nuclear Proteins / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D051028:bcl-2-Associated X Protein
chemical_list: D034961:Antigens, Nuclear; C494826:BAX protein, human; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein