Identification of a 195 kDa protein in the striated rootlet: its expression in ciliated and ciliogenic cells.
Little is known about the molecular composition of the ciliary rootlet. We raised monoclonal antibodies to a crude preparation of striated rootlets isolated from the human oviduct, and obtained a clone (R4109) that specifically labeled the ciliary rootlets. Rootlets associated with the solitary cilium in secretory cells and fibroblasts were also labeled. R4109 identified a 195-kDa protein by immunoblotting. Ciliogenic cells in the oviduct epithelium of young mice were labeled in the globular and/or granular pattern by R4109 by immunofluorescence microscopy. Immunoelectron microscopy showed that they corresponded to fibrogranular complex and dense granule, respectively. The result demonstrated that the 195-kDa protein is a component common to the striated rootlet and dense granule, and thus suggested that dense granules are involved in the rootlet formation.
Hagiwara
H
H
Department of Anatomy and Cell Biology, Gunma University School of Medicine, Maebashi, Japan.
Aoki
T
T
Ohwada
N
N
Fujimoto
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D002923:Cilia / Q000378:metabolism* / Q000648:ultrastructure; D005187:Fallopian Tubes / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D008871:Microvilli / Q000378:metabolism* / Q000648:ultrastructure; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D011506:Proteins