Aluminium triggers genotoxic adaptation to methyl mercuric chloride and ethyl methane sulfonate, but not to maleic hydrazide in plant cells in vivo.
Non-toxic, conditioning doses of aluminium chloride were tested for induction of adaptive response to the genotoxic challenge doses of methyl mercuric chloride (MMCl), maleic hydrazide (MH) and ethyl methane sulfonate (EMS). Embryonic shoot cells of Hordeum vulgare and root meristem cells of Allium cepa were employed as the assay systems. Plant tissues fixed at different recovery hours following the challenge treatments with or without prior Al-conditioning were analyzed for cells with genotoxicity markers that include spindle and/or chromosome aberrations and micronuclei (MNC). The results provided evidence that Al(3+) triggered adaptive response that protected the plant cells from the genotoxicity of MMCl and EMS. Al(3+), however, failed to induce adaptive response against the genotoxicity of MH. A comparison of Al-induced adaptive response with that induced by heavy metals: Cd(2+), Cu(2+), Hg(2+), Ni(2+), Pb(2+), Zn(2+) and oxidative agents: hydrogen peroxide (H(2)O(2)) and paraquat (PQ) pointed to the similarity of Al-adaptive response to that of PQ rather than to other heavy metals or H(2)O(2). Al-induced adaptive response demonstrated in the present study to MMCl and EMS possibly involved antioxidant defense and DNA repair systems, respectively.
Patra
J
J
Department of Botany, Genecology and Tissue Culture Laboratory, Berhampur University, Berhampur 760 007, Orissa, India.
Baisakhi
B
B
Mohapatro
M K
MK
Panda
B B
BB
D000222:Adaptation, Physiological / Q000187:drug effects; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology*; D002869:Chromosome Aberrations; D004260:DNA Repair; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D001467:Hordeum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008300:Maleic Hydrazide / Q000633:toxicity*; D019216:Metals, Heavy / Q000494:pharmacology; D008767:Methylmercury Compounds / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D019697:Onions / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010269:Paraquat / Q000494:pharmacology; D010944:Plants / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
D017607:Aluminum Compounds; D000975:Antioxidants; D002712:Chlorides; D019216:Metals, Heavy; D008767:Methylmercury Compounds; D009153:Mutagens; D000077410:Aluminum Chloride; D005020:Ethyl Methanesulfonate; D006861:Hydrogen Peroxide; D010269:Paraquat; C004925:methylmercuric chloride; D008300:Maleic Hydrazide