title: Intracellular pH changes during oligodendrocyte differentiation in primary culture.
abstract: We have studied the characteristics of pH(i) regulation at different stages of rat oligodendrocyte differentiation in primary culture. pH(i) was measured at 37 degrees C using the pH-sensitive fluorescent probe BCECF. In immature oligodendrocyte progenitor (OLP), three distinct ionic mechanisms were involved in pH(i) regulation: (i) a sodium-independent Cl(-)/HCO(-)(3) exchanger, (ii) a Na(+)/H(+) exchanger and (iii) a voltage-dependent Na(+)-HCO(-)(3) cotransporter. The two latter mechanisms were also detected in more differentiated pro-oligodendrocytes and in mature oligodendrocytes whereas the Cl(-)/HCO(-)(3) exchanger was not active in these two later stages of differentiation. The presence of this Cl(-)/HCO(-)(3) exchanger (that acts as a chronic acidifying mechanism) only in immature OLP maintains in these cells a steady-state pH(i) value significantly lower than values measured in more differentiated cells. The possible involvement of this pH(i) change in triggering cell differentiation is discussed.
authors.affiliation: Laboratoire de Neurobiologie Cellulaire, CNRS, Centre de Neurochimie, Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: Boussouf
authors.forename: S
authors.initials: S
authors.lastname: Gaillard
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D017136:Ion Transport / Q000502:physiology*; D009103:Multiple Sclerosis / Q000378:metabolism / Q000503:physiopathology; D009836:Oligodendroglia / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000502:physiology
chemical_list: D001639:Bicarbonates; D002712:Chlorides; D002245:Carbon Dioxide