2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide: evaluation of the spin trapping properties.
The 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-l-oxide (EMPO), an easily prepared pyrroline-N-oxide has been tested as a free radical scavenger. Spin adducts of superoxide, hydroxyl radical, and other free radicals were characterized in phosphate buffer at pH 7.0 and 5.6. At pH 7 in phosphate buffer, the EMPO/O(2)(-*) spin adduct was estimated to be about five times more persistent than its DMPO analogue. Furthermore, its decay does not produce the EMPO/HO&z.rad; adduct.
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Laboratoire Structure et Réactivité des Espèces Paramagnétiques, CNRS UMR 6517, "Chimie, Biologie et Radicaux Libres", Universités d'Aix-Marseille I et III, Centre de St Jérôme, Service 521, Marseille, France.
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D000818:Animals; D002021:Buffers; D002417:Cattle; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000201:enzymology; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D015394:Molecular Structure; D010710:Phosphates; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000097:blood; D014969:Xanthine Oxidase / Q000378:metabolism
C406273:2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide; D002021:Buffers; D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010710:Phosphates; D011758:Pyrroles; D013113:Spin Labels; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase