Liquid chromatographic determination of hippuric acid for the evaluation of ethacrynic acid as angiotensin converting enzyme inhibitor.
A rapid, simple and interference-free method is described to evaluate the inhibitory effects of organic compounds on the activity of angiotensin converting enzyme irrespective of their acid-base properties. The assay is based on the high performance liquid chromatographic separation of the synthetic substrate hippuryl-L-histidyl-L-leucine, the hydrolysis product hippuric acid and the test compound. Using the new method, the diuretic drug ethacrynic acid was found to act as an inhibitor for the enzyme in a non competitive mode.
Mehanna
A S
AS
Division of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Allied Health Sciences, Boston 02115, USA. amehanna@mcp.edu
Dowling
M
M
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004976:Ethacrynic Acid / Q000032:analysis*; D006626:Hippurates / Q000032:analysis*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D012015:Reference Standards; D015203:Reproducibility of Results
D000806:Angiotensin-Converting Enzyme Inhibitors; D006626:Hippurates; D007703:Peptidyl-Dipeptidase A; D004976:Ethacrynic Acid; C030514:hippuric acid