title: Establishment and analysis of biological characteristics of a human duodenal carcinoma cell line, WDC-1.
abstract: BACKGROUND
  Duodenal carcinoma is very rare and its culture cell lines have rarely been established.
  
  
  METHODS
  Tumor cells separated from a surgically resected primary tumor of duodenal carcinoma were put into culture. The patient was an 81-year-old female and had metastatic lymph nodes. We investigated the biological characteristics of the culture cells including in vitro cell kinetics, karyotype, expression of tumor markers and integrins and tumorigenicity and histology in nude mice.
  
  
  RESULTS
  A new cell line, designated WDC-1, was established. This duodenal carcinoma cell line proliferated in a monolayered sheet with a doubling time of 50 h. The histological findings of the xenograft in nude mice were similar to those of the primary tumor. WDC-1 cells produced carcinoembryonic antigen and expressed 1 integrin and very late antigen (VLA)-4d in vitro.
  
  
  CONCLUSIONS
  A duodenal carcinoma cell line was established, which is rare and may contribute to progress in understanding the biological features of duodenal cancer.
authors.affiliation: Second Department of Surgery, Wakayama Medical School, Kimiidera, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tani
authors.forename: H
authors.initials: H
authors.lastname: Tanimura
authors.forename: H
authors.initials: H
authors.lastname: Terasawa
authors.forename: N
authors.initials: N
authors.lastname: Yamade
authors.forename: Y
authors.initials: Y
authors.lastname: Shono
authors.forename: H
authors.initials: H
authors.lastname: Yamaue
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D018395:CA-19-9 Antigen / Q000378:metabolism; D002272:Carcinoembryonic Antigen / Q000096:biosynthesis; D004379:Duodenal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D016023:Integrins / Q000378:metabolism; D008207:Lymphatic Metastasis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D018395:CA-19-9 Antigen; D002272:Carcinoembryonic Antigen; D016023:Integrins; D019010:Vascular Cell Adhesion Molecule-1