title: Evaluation of the H-point standard additions method (HPSAM) and the generalized H-point standard additions method (GHPSAM) for the UV-analysis of two-component mixtures.
abstract: The H-point standard additions method (HPSAM) and two versions of the generalized H-point standard additions method (GHPSAM) are evaluated for the UV-analysis of two-component mixtures. Synthetic mixtures of anhydrous caffeine and phenazone as well as of atovaquone and proguanil hydrochloride were used. Furthermore, the method was applied to pharmaceutical formulations that contain these compounds as active drug substances. This paper shows both the difficulties that are related to the methods and the conditions by which acceptable results can be obtained.
authors.affiliation: ChemoAC, Pharmaceutical Institute, Vrije Universiteit Brussel, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Hund
authors.forename: D L
authors.initials: DL
authors.lastname: Massart
authors.forename: J
authors.initials: J
authors.lastname: Smeyers-Verbeke
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry; D000962:Antimalarials / Q000737:chemistry*; D000983:Antipyrine / Q000737:chemistry*; D053626:Atovaquone; D002110:Caffeine / Q000737:chemistry*; D000697:Central Nervous System Stimulants / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D005069:Evaluation Studies as Topic; D008956:Models, Chemical; D009285:Naphthoquinones / Q000737:chemistry; D002727:Proguanil / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000962:Antimalarials; D000697:Central Nervous System Stimulants; D009285:Naphthoquinones; D002110:Caffeine; D002727:Proguanil; D000983:Antipyrine; D053626:Atovaquone