title: Detailed monitoring of hematopoietic chimerism in a child treated by adoptive immunotherapy for high risk of relapse after BMT for acute myeloid leukemia.
abstract: We report a case of a 13-year-old boy who was transplanted for relapse of acute myeloid leukemia (AML). A detailed study of hematopoietic chimerism was performed using polymerase chain reaction (PCR) of variable number of tandem repeats (VNTR) at very short time intervals. We used discontinuation of post-transplant immunosuppression and donor lymphocyte infusions (DLI) in order to prevent leukemia relapse that was indicated by a progressive increase in autologous hematopoiesis. Despite the fact that the boy relapsed 10 months after BMT, we could see a significant influence of adoptive immunotherapy on the mixed chimerism status during the post-transplant period.
authors.affiliation: Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Formánková
authors.forename: L
authors.initials: L
authors.lastname: Honzátková
authors.forename: Z
authors.initials: Z
authors.lastname: Sieglová
authors.forename: J
authors.initials: J
authors.lastname: Starý
authors.forename: P
authors.initials: P
authors.lastname: Sedlácek
authors.forename: R
authors.initials: R
authors.lastname: Brdicka
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D016026:Bone Marrow Transplantation; D003131:Combined Modality Therapy; D006410:Hematopoiesis / Q000235:genetics / Q000276:immunology; D006801:Humans; D016219:Immunotherapy, Adoptive; D007951:Leukemia, Myeloid / Q000276:immunology / Q000628:therapy*; D008297:Male; D055502:Secondary Prevention; D018183:Transplantation Chimera; D014184:Transplantation, Homologous