title: Antagonistic activity of Lactobacillus acidophilus LB against intracellular Salmonella enterica serovar Typhimurium infecting human enterocyte-like Caco-2/TC-7 cells.
abstract: To gain further insight into the mechanism by which lactobacilli develop antimicrobial activity, we have examined how Lactobacillus acidophilus LB inhibits the promoted cellular injuries and intracellular lifestyle of Salmonella enterica serovar Typhimurium SL1344 infecting the cultured, fully differentiated human intestinal cell line Caco-2/TC-7. We showed that the spent culture supernatant of strain LB (LB-SCS) decreases the number of apical serovar Typhimurium-induced F-actin rearrangements in infected cells. LB-SCS treatment efficiently decreased transcellular passage of S. enterica serovar Typhimurium. Moreover, LB-SCS treatment inhibited intracellular growth of serovar Typhimurium, since treated intracellular bacteria displayed a small, rounded morphology resembling that of resting bacteria. We also showed that LB-SCS treatment inhibits adhesion-dependent serovar Typhimurium-induced interleukin-8 production.
authors.affiliation: Pathogènes et Fonctions des Cellules Epithéliales Polarisées, Institut National de la Santé et de la Recherche Médicale, Unité 510, Faculté de Pharmacie, Université Paris XI, F-92296 Châtenay-Malabry, France.
authors.forename: M H
authors.initials: MH
authors.lastname: Coconnier
authors.forename: V
authors.initials: V
authors.lastname: Liévin
authors.forename: M
authors.initials: M
authors.lastname: Lorrot
authors.forename: A L
authors.initials: AL
authors.lastname: Servin
mesh_terms: D000199:Actins / Q000187:drug effects; D000898:Antibiosis; D001422:Bacterial Adhesion / Q000187:drug effects; D018938:Caco-2 Cells; D016764:Cell Polarity; D017077:Culture Media, Conditioned / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D020895:Enterocytes / Q000382:microbiology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007421:Intestine, Small / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000502:physiology*; D019779:Salmonella enterica / Q000472:pathogenicity*
chemical_list: D000199:Actins; D017077:Culture Media, Conditioned; D016209:Interleukin-8