Molecular modeling of the oxytocin receptor/bioligand interactions.
Oxytocin is a nonapeptide hormone (CYIQNCPLG-NH2, OT), controlling labor and lactation in mammalian females, via interactions with specific cellular membrane receptors (OTRs). The native hormone is cyclized via a 1-6 disulfide and its receptor belongs to the GTP-binding (G) protein-coupled receptor (GPCR) family, also known as heptahelical transmembrane (7TM) or serpentine receptors. Using a technique combining multiple sequence alignments with available experimental constraints, a reliable OTR model was built. Subsequently, the OTR complexes with a selective agonist [Thr4,Gly7]OT, a selective cyclohexapeptide antagonist L-366,948 and oxytocin itself were modeled and relaxed using a constrained simulated annealing (CSA) protocol. All three ligands seem to prefer similar modes of binding to the receptor, manifested by repeating receptor residues which directly interact with the ligands. Those involved in the three complexes are putative helices: TM3: R113, K116, Q119, M123; TM4: Q171, and TM5: I201 and T205. Most of them are the equivalent residues/positions to those found in our earlier studies, regarding related vasopressin V2 receptor/bioligand interactions.
Politowska
E
E
Faculty of Chemistry, University of Gdańsk, Poland.
Czaplewski
C
C
Ciarkowski
J
J
D000595:Amino Acid Sequence; D001665:Binding Sites; D005260:Female; D006727:Hormone Antagonists / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010121:Oxytocin / Q000819:agonists / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000737:chemistry; D010456:Peptides, Cyclic / Q000737:chemistry; D011247:Pregnancy; D011487:Protein Conformation; D018045:Receptors, Oxytocin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D006727:Hormone Antagonists; D008024:Ligands; D010456:Peptides, Cyclic; D018045:Receptors, Oxytocin; C066873:L 366948; D010121:Oxytocin; C045427:oxytocin, Thr(4)-Gly(7)-