The preparation, characterisation, and DNA adduct profile of 2-amino-2-methyl-3-butanoneoximedichloroplatinum(II), a platinum(II) complex designed to bind to GpA sequences of DNA.
The complex, 2-amino-2-methyl-3-butanoneoximedichloroplatinum(II), [Pt(ambo)Cl2], was chosen because of its potential to bind to GpA sequences of duplex DNA. Crystals of [Pt(ambo)Cl2] are monoclinic, space group, P2(1)/n, a = 6.799(4), b = 17.642(5), c = 8.193(2) A, beta = 102.10(3) degrees, Z = 4, R = 0.033 (1864 F). The binding of [Pt(ambo)Cl2] to salmon-sperm DNA was studied using enzymatic digestion and HPLC analysis. [Pt(ambo)Cl2] was found to form fewer GpG and ApG intrastrand adducts and more monofunctional adducts than [Pt(en)Cl2]. Binding to GpA sequences could not be established, but [Pt(ambo)Cl2] forms substantially more adducts with adenine than does [Pt(en)Cl2].
Ling
E C
EC
School of Chemistry, University of Sydney, NSW, Australia.
Allen
G W
GW
Vickery
K
K
Hambley
T W
TW
D000225:Adenine / Q000378:metabolism; D001665:Binding Sites; D001669:Biochemical Phenomena; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000031:analogs & derivatives; D003461:Crystallography; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts / Q000138:chemical synthesis* / Q000378:metabolism*; D004798:Enzymes; D006860:Hydrogen Bonding; D015394:Molecular Structure; D009841:Oligonucleotides / Q000378:metabolism*; D009944:Organoplatinum Compounds / Q000138:chemical synthesis* / Q000378:metabolism* / Q000494:pharmacology* / Q000592:standards; D012015:Reference Standards
C406386:2-amino-2-methyl-3-butanoneoximedichloroplatinum(II); D018736:DNA Adducts; D004798:Enzymes; D009841:Oligonucleotides; D009944:Organoplatinum Compounds; D004247:DNA; D000225:Adenine; D002945:Cisplatin