Lipid removal from human serum samples.
The efficacy of lipid removal from human serum samples obtained by using Cleanascite HC, a commercially available product, was compared to that obtained by the standard chloroform method. Separate samples of 21 frozen, banked human serum samples used in the preparation of samples for proficiency testing were treated with either Cleanascite HC or chloroform. The lipid content was measured before and after treatment. The total percentages of lipid removed ranged from 61 to 70% with Cleanascite HC and from 60 to 62% with chloroform. The advantage of Cleanascite HC over chloroform is based on the simplicity of the procedure with Cleanascite HC without the environmental concerns inherent in the use of chloroform. In 15 serum samples known to contain antibodies to treponemal and nontreponemal syphilis antigens, Cleanascite HC bound some immunoglobulin, but with only minimal loss of reactivity in the serologic tests for syphilis. Cleanascite HC is therefore an acceptable alternative to chloroform for lipid reduction in human serum samples.
Castro
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Division of AIDS, STD, and TB Laboratory Research, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. ajc5@cdc.gov
Morrill
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Pope
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V
D002725:Chloroform; D006801:Humans; D008055:Lipids / Q000097:blood / Q000302:isolation & purification*; D012822:Silicon Dioxide; D013587:Syphilis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000378:metabolism
D008055:Lipids; D012822:Silicon Dioxide; D002725:Chloroform