An isocratic LC method for the simultaneous determination of vitamins A, C, E and beta-carotene.
An isocratic liquid chromatographic method for the separation and simultaneous determination of retinyl acetate, propionate or palmitate (esters of vitamin A), beta-carotene (pro-vitamin A), ascorbic acid (vitamin C) and DL-alpha-tocopherol (vitamin E) is described. Samples are analysed by means of a reversed-phase column (LiChrospher 100 RP-18), using methanol as mobile phase. The UV Vis detector used was set at a wavelength of 300 nm and switched to 450 nm at 17 min, allowing the determination of beta-carotene. These vitamins were separated within 25 min and the detection limits ranged from 7 (beta-carotene) to 65 ng ml(-1) (ascorbic acid).
Paulo
M G
MG
Faculdade de Farmácia da Universidade de Lisboa, Unidade de Ciências e Tecnologia Farmacêuticas, Portugal.
Marques
H M
HM
Morais
J A
JA
Almeida
A J
AJ
D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005079:Excipients / Q000737:chemistry; D000432:Methanol; D012044:Regression Analysis; D015203:Reproducibility of Results; D013997:Time Factors; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*; D019207:beta Carotene / Q000032:analysis*
D005079:Excipients; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; D001205:Ascorbic Acid; D000432:Methanol