[Staphylococcus aureus-lysing enzymes: isolation and some properties of lysostaphine].
The authors investigated a procedure for isolating lysostaphine, a complex of enzymes that lyse the cells of virtually any S. aureus strains in the fermenter and large bottles. It was shown that the activity of the enzyme in the fermenter might be much higher than in the flask. The enzyme can be concentrated on the hollow fibers, purified on the ion exchanger and kept without decreasing its activity within a year. The process can be scaled and, lysostaphine may be used in the treatment of man and animals after its proper biological tests.
Surovtsev
V I
VI
Gusev
V V
VV
Borozdina
M A
MA
Paderin
Iu P
IuP
Khatiushin
Iu I
IuI
Chuprunov
V P
VP
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001433:Bacteriolysis / Q000187:drug effects*; D004339:Drug Compounding / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008248:Lysostaphin / Q000302:isolation & purification* / Q000494:pharmacology; D010455:Peptides; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects*; D013212:Staphylococcus epidermidis / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D010455:Peptides; D008248:Lysostaphin