[Temperature-dependent changes in immunochemical properties of lipopolysaccharides of yersinia pestis].
The preparations of pilopolysaccharides (LPS) of virulent and avirulent Y. pestis strains, which have a different composition, were cultivated at different temperatures. Despite the cultivation temperature of parental cells, all LPS preparation inhibited the passive hemagglutination reaction (PHR) of the red blood cells sensitized by LPS isolated from the cultures grown at 26 degrees C by using homologous antisera. In contrast, the homologous system consisting of the red blood cells sensitized by LPS from the EV NIIEG, cultured at 37 degrees C, and the antiserum to these cells proved to be more specific and it was inhibited only by the homologous LPS isolated from the strain EV NIIEG. The similar reaction of the interaction of the red blood cells sensitized by high temperature LPS agents from plasmid-free strains with the same serum was inhibited by all plague LPS preparations. The LPS preparations from the strains Y. pestis 1146 and Y. pseudotuberculosis 9532 obtained when cultivated at 37 degrees C. RHR of the red blood cells sensitized by these preparations was inhibited by homologous LPS irrespective of the temperature of cell cultivation. In all cases, the reaction was specific for Yersinia strains and it was inhibited by LPS from Ra-Rd2 cultures, variants of Salmonella and E. coli.
Gremiakova
T A
TA
Volkovoĭ
K I
KI
Shaĭkhutdinova
R Z
RZ
Stepanov
A V
AV
D000942:Antigens, Bacterial / Q000276:immunology*; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D013696:Temperature; D014774:Virulence; D015010:Yersinia pestis / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides