Latexin, a carboxypeptidase A inhibitor, is expressed in rat peritoneal mast cells and is associated with granular structures distinct from secretory granules and lysosomes.
Latexin, a protein possessing inhibitory activity against rat carboxypeptidase A1 (CPA1) and CPA2, is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Although latexin also inhibits mast-cell CPA (MCCPA), the expression of latexin in rat mast cells has not previously been confirmed. In the present study we examined the expression and subcellular localization of latexin in rat peritoneal mast cells. Western blot and reverse-transcriptase-mediated PCR analyses showed that latexin was contained and expressed in the rat peritoneal mast cells. Immunocytochemically, latexin immunofluorescence was localized on granular structures distinct from MCCPA-, histamine- or cathepsin D-immunopositive granules. Immunoelectron microscopy revealed that latexin was associated with a minority population of granules. The latexin-associated granules were separated from MCCPA- or histamine-containing granules on a self-generating density gradient of polyvinylpyrrolidone-coated silica-gel particles (Percoll). Treatments with high ionic strength and heparinase released latexin from the granules, suggesting that latexin is non-covalently associated with a heparin-like component of the granules. MCCPA and histamine were released from the mast cells after non-immunological and immunological stimulation with compound 48/80, A23187 and anti-IgE antibody, whereas latexin was not released. These results show that latexin is synthesized in rat peritoneal mast cells and suggest that it is associated with a unique type of intracellular granules distinct from MCCPA- and histamine-containing secretory granules and lysosomes.
Uratani
Y
Y
Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194- 8511, Japan. uraty@libra.ls.m-kagaku.co.jp
Takiguchi-Hayashi
K
K
Miyasaka
N
N
Sato
M
M
Jin
M
M
Arimatsu
Y
Y
D000818:Animals; D000941:Antigens / Q000494:pharmacology*; D001483:Base Sequence; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D043422:Carboxypeptidases A; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019763:Heparin Lyase / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism; D007150:Immunohistochemistry; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism*; D008407:Mast Cells / Q000201:enzymology / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats
D000941:Antigens; D017931:DNA Primers; D004791:Enzyme Inhibitors; C086812:Lxn protein, rat; D006632:Histamine; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D019763:Heparin Lyase