Isolation of U(VI) reduction-deficient mutants of Shewanella putrefaciens.
A U(VI) reduction-deficient mutant (Urr) screening technique was developed and combined with chemical mutagenesis procedures to identify a Urr mutant of Shewanella putrefaciens strain 200. The Urr mutant lacked the ability to grow anaerobically on U(VI) and NO(2)(-), yet retained the ability to grow anaerobically on eight other compounds as terminal electron acceptor. All 11 members of previously isolated sets of Fe(III) and Mn(IV) reduction-deficient mutants of S. putrefaciens 200 displayed Urr-positive phenotypes with the Urr screen and were capable of anaerobic growth on U(VI). This is the first reported isolation of a respiratory mutant that is unable to grow anaerobically on U(VI) as terminal electron acceptor.
Wade
R
R
School of Biology, Georgia Institute of Technology, Atlanta, GA 30332-0230, USA.
DiChristina
T J
TJ
D000693:Anaerobiosis; D003470:Culture Media; D016296:Mutagenesis; D009154:Mutation; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D020614:Shewanella putrefaciens / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D014501:Uranium / Q000378:metabolism*
D003470:Culture Media; D014501:Uranium