Synthesis of the fatty sterol bound protein for a new sterol antibody.
For the purpose of applying the particular antibodies as a new diagnostic procedure for atherosclerosis and related diseases, we successfully achieved the synthesis of the fatty sterol with a linker, then linked the target protein to this sterol. Synthesis was started from pregnenolone and achieved by the Grignard reaction with pentenyl magnesium bromide, regioselective photoaddition of thiolacetic acid toward the 25-double bond, esterification of 3-OH with linoleic anhydride, in situ conjunction of the cross-linker (MBS) to the thiol group after selective deprotection from its acetyl ester, and finally by the reaction with protein such as KLH or albumin through this linker.
Kim
B J
BJ
Departmnent of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Japan.
Yamada
S
S
Funada
T
T
Kadoma
Y
Y
Morita
H
H
D000276:Adjuvants, Immunologic / Q000138:chemical synthesis / Q000378:metabolism; D000418:Albumins / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000378:metabolism; D006433:Hemocyanins / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011284:Pregnenolone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism; D011506:Proteins / Q000138:chemical synthesis / Q000276:immunology*; D013261:Sterols / Q000138:chemical synthesis / Q000276:immunology* / Q000378:metabolism
D000276:Adjuvants, Immunologic; D000418:Albumins; D000906:Antibodies; D000941:Antigens; D003432:Cross-Linking Reagents; D011506:Proteins; D013261:Sterols; D011284:Pregnenolone; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin