title: Ontogeny and regulation of variant thyroid hormone receptor isoforms in developing rat testis.
abstract: High affinity-low capacity nuclear triiodothyronine (T3) receptors (TRs), identified as a product of c-erbAalpha proto-oncogene, are expressed in prepubertal rat Sertoli cell. At this age, exogenous T3 treatment as well as hypothyroidism affects Sertoli cell functions. We examined the ontogenetic expression pattern of TRs in the rat testis. Northern analysis confirms that TRs are expressed at high level from fetal development until prepubertal period. RNase protection analysis demonstrates that TRalpha2, the variant isoform of TRalpha1, is constitutively expressed at all ages, while TRalpha3 is absent in the adult gonad. While TRalpha1 and TRalpha2 expression declines during development, Rev-erbAalpha (Rev), the antisense mRNA encoded by the same c-erbAalpha genomic locus, increases beginning 5 days after birth and maximizing in adulthood. TRalpha1, TRalpha2, and Rev mRNAs do not appear to be directly regulated by thyroid hormone in testis; however, short-term neonatal hypothyroidism leads to the expression of TRalpha1 and its variant in adult testis, which is absent in control coeval animals. Thus, during development of rat testis, the levels of messages of genes encoded in the c-erbAalpha. genomic locus have different ontogenetic control. The ontogenetic profile of TRalpha1 and its variant isoforms within the seminiferous epithelium suggests that these receptors are involved in the differentiation of the male gonad.
authors.affiliation: Department of Experimental Medicine, University of L'Aquila, Coppito, Italy.
authors.forename: E A
authors.initials: EA
authors.lastname: Jannini
authors.forename: E
authors.initials: E
authors.lastname: Carosa
authors.forename: N
authors.initials: N
authors.lastname: Rucci
authors.forename: E
authors.initials: E
authors.lastname: Screponi
authors.forename: M
authors.initials: M
authors.lastname: D'Armiento
mesh_terms: D000375:Aging; D000818:Animals; D015152:Blotting, Northern; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D013737:Testis / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine