title: 2-Substituted tryptamines: agents with selectivity for 5-HT(6) serotonin receptors.
abstract: Several 2-alkyl-5-methoxytryptamine analogues were designed and prepared as potential 5-HT(6) serotonin agonists. It was found that 5-HT(6) receptors accommodate small alkyl substituents at the indole 2-position and that the resulting compounds can bind with affinities comparable to that of serotonin. In particular, 2-ethyl-5-methoxy-N, N-dimethyltryptamine (8) binds with high affinity at human 5-HT(6) receptors (K(i) = 16 nM) relative to 5-HT (K(i) = 75 nM) and was a full agonist, at least as potent (8: K(act) = 3.6 nM) as serotonin (K(act) = 5.0 nM), in activating adenylate cyclase. Compound 8 displays modest affinity for several other populations of 5-HT receptors, notably h5-HT(1A) (K(i) = 170 nM), h5-HT(1D) (K(i) = 290 nM), and h5-HT(7) (K(i) = 300 nM) receptors, but is otherwise quite selective. Compound 8 represents the first and most selective 5-HT(6) agonist reported to date. Replacing the 2-ethyl substituent with a phenyl group results in a compound that retains 5-HT(6) receptor affinity (i.e., 10: K(i) = 20 nM) but lacks agonist character. 2-Substituted tryptamines, then, might allow entry to a novel class of 5-HT(6) agonists and antagonists.
authors.affiliation: Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond, Virginia 23298, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Glennon
authors.forename: M
authors.initials: M
authors.lastname: Lee
authors.forename: J B
authors.initials: JB
authors.lastname: Rangisetty
authors.forename: M
authors.initials: M
authors.lastname: Dukat
authors.forename: B L
authors.initials: BL
authors.lastname: Roth
authors.forename: J E
authors.initials: JE
authors.lastname: Savage
authors.forename: A
authors.initials: A
authors.lastname: McBride
authors.forename: L
authors.initials: L
authors.lastname: Rauser
authors.forename: S
authors.initials: S
authors.lastname: Hufeisen
authors.forename: D K
authors.initials: DK
authors.lastname: Lee
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation; D006801:Humans; D008732:Methoxydimethyltryptamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D011869:Radioligand Assay; D011985:Receptors, Serotonin / Q000187:drug effects*; D012702:Serotonin Antagonists / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D017366:Serotonin Receptor Agonists / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014363:Tryptamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
chemical_list: C406233:2-ethyl-5-methoxy-N,N-dimethyltryptamine; D008732:Methoxydimethyltryptamines; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; D014363:Tryptamines; C097216:serotonin 6 receptor; D000262:Adenylyl Cyclases