Human herpesvirus 8 and Epstein Barr-virus in a cutaneous B-cell lymphoma and a malignant cell line established from the blood of an AIDS patient.
Human Herpesvirus 8 (HHV-8) has been consistently associated with Primary Effusion Lymphoma (PEL or body-cavity-based lymphoma) but not with other lymphomas. This paper reports on an AIDS patient without obvious malignant effusion in body cavities but with a cutaneous lymphoma where HHV-8 and Epstein-Barr virus (EBV) were detected by PCR and electron microscopy. Both viruses were also detected in all the cells of a malignant cell line (BBG1) established from the patient's peripheral blood mononuclear cells. As in PEL and PEL-derived cell lines, both the tumor and the lines lacked B-antigen expression in immunological studies but were of the same B origin as shown by clonal immunoglobulin gene rearrangements. In contrast to other co-infected cell lines, BBG1 and subclones spontaneously expressed the HHV-8 lytic antigens p40, p27, p60 and the EBV transforming latent antigen EBNA2. These data suggest that the clinical and biological features of HHV-8-and EBV-associated lymphomas could be wider than has been described to date in PEL particularly with the in vivo presence of circulating malignant dually-infected cells engaged in a spontaneous HHV-8 lytic infection.
Morand
P
P
Laboratoire de Virologie Médicale Moléculaire (RHAP), Grenoble, France. Patrice.Morand@ujf-grenoble.fr
Buisson
M
M
Collandre
H
H
Chanzy
B
B
Genoulaz
O
O
Bourgeat
M J
MJ
Pinel
N
N
Leclercq
P
P
Leroux
D
D
Marechal
V
V
Fritsch
L
L
Ruigrok
R
R
Seigneurin
J M
JM
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000150:complications / Q000473:pathology / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000209:etiology / Q000821:virology*; D016393:Lymphoma, B-Cell / Q000209:etiology / Q000821:virology*; D008969:Molecular Sequence Data; D012878:Skin Neoplasms / Q000209:etiology / Q000821:virology*; D014407:Tumor Cells, Cultured