title: Rapid confirmation/quantitation of cocaine and benzoylecgonine in urine utilizing high performance liquid chromatography and tandem mass spectrometry.
abstract: A rapid, but sensitive and selective method for confirmation and quantitation of benzoylecgonine (BZE) and cocaine (COC) in urine by fast-gradient liquid chromatography/tandem mass spectrometry (LC/MS/MS) is described. The chromatographic separation was performed on a reversed phase column employing fast-gradient techniques. Matrix prepared standards, blanks, and QC's were filtered then aliquots were transferred into a 96 well plate. Injection volumes of 25 microL were made onto the analytical column, with the flow diverted from the atmospheric pressure ionization source for the first 0.5 min of the analysis. Simultaneous multiple reaction monitoring (MRM) of three discrete transitions for each compound were used to identify BZE and COC. Quantitation was achieved utilizing the most prominent parent-daughter transition and internal standard calibration techniques. The coefficients of variation (CV) for the analysis of these drugs ranged from 0.6% to 6.8% at a concentration of 150 ng/mL (n = 155). This method suggests that fast-gradient LC/MS/MS may be suitable for routine confirmation of immunoassay cocaine-positive samples.
authors.affiliation: Drug Metabolism Technology Department, Pfizer Inc., Groton, Connecticut 06340, USA. Patrick_Jeanville@groton.pfizer.com
authors.forename: P M
authors.initials: PM
authors.lastname: Jeanville
authors.forename: E S
authors.initials: ES
authors.lastname: Estapé
authors.forename: S R
authors.initials: SR
authors.lastname: Needham
authors.forename: M J
authors.initials: MJ
authors.lastname: Cole
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D015203:Reproducibility of Results; D015813:Substance Abuse Detection
chemical_list: D007202:Indicators and Reagents; C005618:benzoylecgonine; D003042:Cocaine