title: Proteolytic processing and degradation of human presenilin-1 expressed in yeast.
abstract: Numerous mutations causing early-onset familial Alzheimer's disease have been identified in the presenilin-1 gene. Presenilin-1 protein is produced as a 47 kDa holoprotein and proteolytically processed to an N-terminal 28 kDa and a C-terminal 19 kDa fragments by unidentified presenilinase in mammalian cells. We have demonstrated that this proteolytic processing also occurs in yeast. We also show that degradation of C-terminal fragment of presenilin-1 is dependent of proteasomal function. This yeast system will be a good tool to identify presenilinase and to study the role of presenilin-1 in amyloid precursor protein processing.
authors.affiliation: Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Song
authors.forename: M
authors.initials: M
authors.lastname: Ohba
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Honda
authors.forename: A
authors.initials: A
authors.lastname: Takashima
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism; D009154:Mutation; D053764:Presenilin-1; D046988:Proteasome Endopeptidase Complex; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D009097:Multienzyme Complexes; C508853:PSEN1 protein, human; D053764:Presenilin-1; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex