Novel process for enzymatic hydrolysis of proteins in an aqueous two-phase system for the production of peptide mixture.
A novel process for the production of peptide mixtures is proposed. Biologically active peptides were synthesized using a thermolysin-catalyzed hydrolysis of a corn protein (zein) in an aqueous two-phase system. The mixture of peptides which was selectively recovered from the dextran-rich bottom phase had a higher angiotensin-converting enzyme (ACE) inhibitory activity than native zein.
Murakami
Y
Y
Department of Chemical Engineering, Waseda University, Tokyo, Japan.
Hirata
A
A
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D006868:Hydrolysis; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000187:drug effects / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry; D015028:Zein / Q000378:metabolism* / Q000494:pharmacology
D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D015028:Zein; D010450:Endopeptidases; D007703:Peptidyl-Dipeptidase A