Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine and 5-(hydroxymethyl) uracil in smokers.
Cigarette smoke is known to generate free radicals by various mechanisms. In this study involving 30 non-smokers and 30 smokers, we show that urinary excretion of 5-(hydroxymethyl) uracil (HMUra) was not different in the two groups (6.54+/-2.07 vs. 6.70+/-1.68 nmol/mmol creatinine). In contrast, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) excretion increased by 16% (1.16+/-0.35 vs. 1.35+/-0.50 nmol/mmol creatinine, p = 0.039). Results concerning 8-oxo-dGuo are in agreement with those of previous studies. We observed significant multiple correlations between HMUra and creatinine (r(p) = 0.44), BMI (r(p) = -0.27) and nicotine derivatives (r(p) = 0.26). Multiple correlation analysis showed relations between 8-oxo-dGuo on the one hand, and: creatinine (r(p) = 0.36), nicotine derivatives (r(p) = 0.29), BMI (r(p) = -0.24) on the other.
Pourcelot
S
S
LBSO-LCR CEA n8, Faculté de Médecine-Pharmacie, Domaine de la Merci, La Tronche, France.
Faure
H
H
Firoozi
F
F
Ducros
V
V
Tripier
M
M
Hee
J
J
Cadet
J
J
Favier
A
A
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D016022:Case-Control Studies; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010432:Pentoxyl / Q000031:analogs & derivatives* / Q000652:urine; D012907:Smoking / Q000378:metabolism / Q000652:urine*
D005609:Free Radicals; C008055:5-hydroxymethyluracil; D010432:Pentoxyl; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine