Epitopes involved in antibody-mediated protection from Ebola virus.
To determine the ability of antibodies to provide protection from Ebola viruses, monoclonal antibodies (mAbs) to the Ebola glycoprotein were generated and evaluated for efficacy. We identified several protective mAbs directed toward five unique epitopes on Ebola glycoprotein. One of the epitopes is conserved among all Ebola viruses that are known to be pathogenic for humans. Some protective mAbs were also effective therapeutically when administered to mice 2 days after exposure to lethal Ebola virus. The identification of protective mAbs has important implications for developing vaccines and therapies for Ebola virus.
Wilson
J A
JA
Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702-5011, USA.
Hevey
M
M
Bakken
R
R
Guest
S
S
Bray
M
M
Schmaljohn
A L
AL
Hart
M K
MK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000915:Antibody Affinity; D000956:Antigens, Viral / Q000276:immunology; D001667:Binding, Competitive; D003165:Complement System Proteins / Q000276:immunology; D029043:Ebolavirus / Q000276:immunology* / Q000502:physiology; D000939:Epitopes / Q000276:immunology; D005260:Female; D019142:Hemorrhagic Fever, Ebola / Q000517:prevention & control* / Q000628:therapy; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D013047:Specific Pathogen-Free Organisms; D014759:Viral Envelope Proteins / Q000276:immunology*; D010948:Viral Plaque Assay
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus; D003165:Complement System Proteins