title: Plasmid curing effect of trovafloxacin.
abstract: The effect of sub-inhibitory concentrations of trovafloxacin, a recently developed fluoroquinolone molecule, on the capability of Escherichia coli cells to maintain three different types of plasmids has been investigated by a number of approaches, including the quantification of the loss of plasmid-borne functions and of plasmid DNA by quantitative PCR. The results obtained demonstrate that at concentrations ranging from the MIC to 1/8 of the MIC, trovafloxacin induces a clear, albeit incomplete, 'episome-curing' effect which was observed with plasmids differing in copy number, size and nature of the replication origin of the episome. This effect was most likely not due to an alteration of DNA supercoiling.
authors.affiliation: Laboratory of Genetics, Department of Biology MCA, University of Camerino, 62032, Camerino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Brandi
authors.forename: M
authors.initials: M
authors.lastname: Falconi
authors.forename: S
authors.initials: S
authors.lastname: Ripa
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D004278:DNA, Superhelical / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D024841:Fluoroquinolones; D008826:Microbial Sensitivity Tests; D009287:Naphthyridines / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D000890:Anti-Infective Agents; D004278:DNA, Superhelical; D024841:Fluoroquinolones; D009287:Naphthyridines; C080163:trovafloxacin