title: Alterations of DNA methylation patterns in germ cells and Sertoli cells from developing mouse testis.
abstract: In situ alterations of DNA methylation were studied between 14 d postcoitum and 4 d postpartum in Sertoli cells and germ cells from mouse testis, using anti-5-methylcytosine antibodies. Compared to cultured fibroblasts, Sertoli cells display strongly methylated juxtacentromeric heterochromatin, but hypomethylated chromatids. Germ cells always possess hypomethylated heterochromatin, whereas their euchromatin passes from a demethylated to a strongly methylated status between days 16 and 17 postcoitum. This hypermethylation occurs in the absence of DNA replication, germ cells being blocked in the G(0)-G(1) phase from day 15 postcoitum to birth. The DNA hypermethylation of germ cells is maintained until birth and could be visualized on both chromatids of metaphase chromosomes at the first postpartum cell division. Subsequently, the DNA hypermethylation is lost semiconservatively, being replaced by a methylation pattern recalling the typical fibroblast pattern. These alterations of DNA methylation follow a strict chronology, are chromosome structure and cell-type dependent, and may underlie profound changes of genome function.
authors.affiliation: Département de Radiobiolgie et Radiopathologie, DSV/CEA, Fontenay-aux-Roses France.
authors.forename: H
authors.initials: H
authors.lastname: Coffigny
authors.forename: C
authors.initials: C
authors.lastname: Bourgeois
authors.forename: M
authors.initials: M
authors.lastname: Ricoul
authors.forename: J
authors.initials: J
authors.lastname: Bernardino
authors.forename: A
authors.initials: A
authors.lastname: Vilain
authors.forename: A
authors.initials: A
authors.lastname: Niveleau
authors.forename: B
authors.initials: B
authors.lastname: Malfoy
authors.forename: B
authors.initials: B
authors.lastname: Dutrillaux
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics; D002478:Cells, Cultured; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D002842:Chromatids / Q000235:genetics / Q000378:metabolism; D002871:Chromosome Banding; D002875:Chromosomes / Q000235:genetics / Q000378:metabolism*; D018899:CpG Islands / Q000235:genetics*; D019175:DNA Methylation; D004261:DNA Replication / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006570:Heterochromatin / Q000235:genetics / Q000378:metabolism; D007399:Interphase; D008297:Male; D008677:Metaphase; D051379:Mice; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*; D013737:Testis / Q000254:growth & development; D013997:Time Factors
chemical_list: D006570:Heterochromatin