title: Alterations in T lymphocyte activity following chemical sympathectomy in young and old Fischer 344 rats.
abstract: In aged Fischer 344 (F344) rats, sympathetic noradrenergic (NA) innervation of the spleen is markedly diminished compared with young rats. To determine if diminished NA innervation can still provide functional signals to splenic T cells, young (3 months old) and old (17 months old) F344 rats were treated with the NA-selective neurotoxin, 6-hydroxydopamine (6-OHDA) to destroy peripheral NA nerve fibers. In 3-month-old rats, no alterations in spleen cell Con A-induced T cell proliferation, IL-2 or IFN-gamma production were observed up to 15 days after sympathectomy, when splenic NE was maximally depleted. By 21 days post-sympathectomy, when NE levels had partially recovered, Con A-induced proliferation and IFN-gamma production, but not IL-2 production, were reduced in sympathectomized animals. After day 21 post-sympathectomy, no alterations in T cell functions were observed in sympathectomized animals. In 17-month-old rats, spleen cell Con A-induced proliferation and IL-2 production were reduced 5 days after sympathectomy in the absence of changes in CD5+ T cells or IFN-gamma production. Desipramine pretreatment, to block 6-OHDA uptake and prevent sympathectomy, completely blocked the 6-OHDA-induced effects, demonstrating that the destruction of NA nerve fibers is required. After day 5 post-sympathectomy, no sympathectomy-induced alterations in Con A-induced T cell functions were observed in old animals. These differences between young and old rats demonstrate that old animals are more susceptible to loss of sympathetic NA innervation, perhaps because compensatory mechanisms are limited. The sympathectomy-induced reduction in T cell proliferation indicates that splenic NA innervation in old animals, though diminished, can exert a positive regulatory influence on T lymphocyte function. Further study of sympathetic neural-immune interactions in the aged rat may provide a means to improve T cell responsiveness in aging.
authors.affiliation: Department of Neurobiology and Anatomy and the Center for Psychoneuroimmunology, University of Rochester School of Medicine and Dentistry, NY 14642, USA. kelley_madden@urmc.rochester.edu
authors.forename: K S
authors.initials: KS
authors.lastname: Madden
authors.forename: S Y
authors.initials: SY
authors.lastname: Stevens
authors.forename: D L
authors.initials: DL
authors.lastname: Felten
authors.forename: D L
authors.initials: DL
authors.lastname: Bellinger
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003891:Desipramine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D016627:Oxidopamine / Q000037:antagonists & inhibitors; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000294:innervation / Q000378:metabolism; D013563:Sympathectomy, Chemical; D017779:Sympathetic Fibers, Postganglionic / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism
chemical_list: D003208:Concanavalin A; D016627:Oxidopamine; D003891:Desipramine; D009638:Norepinephrine