title: Role of cytosine deaminase and beta-alanine-pyruvate transaminase in pyrimidine base catabolism by Burkholderia cepacia.
abstract: A determination of the possible role of the salvage enzyme cytosine deaminase or beta-alanine-pyruvate transaminase in the catabolism of the pyrimidine bases uracil and thymine by the opportunistic pathogen Burkholderia cepacia ATCC 25416 was undertaken. It was of interest to learn whether these enzymes were influenced by cell growth on pyrimidine bases and their respective catabolic products to the same degree as the pyrimidine reductive catabolic enzymes were. It was found that cytosine deaminase activity was influenced very little by cell growth on the pyrimidines tested. Using glucose as the carbon source, only B. cepacia growth on 5-methylcytosine as a nitrogen source increased deaminase activity by about three-fold relative to (NH4)2SO4-grown cells. In contrast, the activity of beta-alanine-pyruvate transaminase was observed to be at least double in glucose-grown ATCC 25416 cells when pyrimidine bases and catabolic products served as nitrogen sources instead of (NH4)2SO4. Transaminase activity in the B. cepacia glucose-grown cells was maximal after the strain was grown on either uracil or 5-methylcytosine as a nitrogen source compared to (NH4)2SO4-grown cells. A possible role for beta-alanine-pyruvate transaminase in pyrimidine base catabolism by B. cepacia would seem to be suggested from the similarity in how its enzyme activity responded to cell growth on pyrimidine bases and catabolic products when compared to the response of the three reductive catabolic enzymes.
authors.affiliation: Department of Chemistry and Biochemistry, South Dakota State University, Brookings 57007, USA. westt@cc.sdstate.edu
authors.forename: T P
authors.initials: TP
authors.lastname: West
mesh_terms: D016956:Burkholderia cepacia / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D043525:Cytosine Deaminase; D009700:Nucleoside Deaminases / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D000637:Transaminases / Q000378:metabolism*; D051278:beta-Alanine-Pyruvate Transaminase
chemical_list: D003470:Culture Media; D011743:Pyrimidines; D000637:Transaminases; D051278:beta-Alanine-Pyruvate Transaminase; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase; C030986:pyrimidine