A flagellar gene cluster from the oral spirochaete Treponema maltophilum.
A flagellar gene cluster from the oral spirochaete Treponema maltophilum ATCC 51939T was cloned. Sequence analysis revealed six putative ORFs, two of which encode the flagellar subunit proteins FlaB2 (286 aa) and FlaB3 (285 aa). Northern blot analysis revealed two flagellin transcripts with the expected size of monocistronic mRNAs. Sequence analysis and primer extension experiments indicated that the transcription of the flaB2 gene is directed by a sigma28-like FliA factor. Using fliA and fliA+ Escherichia coli K-12 strains, it was shown that flaB2 expression in E. coli required the sigma28 factor using an initiation site identical to that in Treponema maltophilum. Primer extension analysis revealed two transcriptional start sites 5' of the flaB3 gene, a strong promoter with a sigma28-like -10 promoter element and a weak promoter with a putative sigma54 promoter consensus sequence. Downstream of flaB3, a putative fliD homologue was found, probably encoding the flagellar cap protein of Treponema maltophilum. Flagellin-gene-specific DNA probes hybridized to all 13 Treponema strains investigated, whereas a fliD-specific DNA probe only hybridized to Treponema maltophilum, other treponemal group IV isolates and Treponema brennaborense.
Heuner
Klaus
K
Institut für Mikrobiologie und Hygiene1 and Institut für Pharmakologie und Toxikologie2, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Dorotheenstr. 96, 10117 Berlin, Germany.
Große
Karin
K
Institut für Mikrobiologie und Hygiene1 and Institut für Pharmakologie und Toxikologie2, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Dorotheenstr. 96, 10117 Berlin, Germany.
Schade
Rüdiger
R
Institut für Mikrobiologie und Hygiene1 and Institut für Pharmakologie und Toxikologie2, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Dorotheenstr. 96, 10117 Berlin, Germany.
Göbel
Ulf B
UB
Institut für Mikrobiologie und Hygiene1 and Institut für Pharmakologie und Toxikologie2, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Dorotheenstr. 96, 10117 Berlin, Germany.
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017931:DNA Primers; D005407:Flagella / Q000235:genetics* / Q000378:metabolism; D005408:Flagellin / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014208:Treponema / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D017931:DNA Primers; C064971:FliA protein, Bacteria; D012808:Sigma Factor; D005408:Flagellin; C416532:flaB flagellin; C076733:FlaD protein, Bacteria