Patterns of cell death induced by eluates from denture base acrylic resins in U-937 human monoblastoid cells.
The purpose of this study was to investigate in vitro the apoptosis- and necrosis-inducing potential of eluates from three heat-polymerized and four autopolymerized poly(methyl methacrylate)-based denture base resins. Our hypothesis was that the rate of cell death by apoptosis and/or necrosis induced by such denture base resins could be an important indicator of their cytotoxicity degree. U-937 human monoblastoid cells were exposed for 24 h and 48 h to eluates of 0.1 g/ml, 0.2 g/ml, 0.4 g/ml, and 0.8 g/ml extracted for 24 h and 48 h. The characteristics of apoptosis and necrosis were evaluated by flow cytometry and light and electron microscopy. Eluates from all resins enhanced cell death by apoptosis and necrosis in U-937 cells in a dose- and time-dependent fashion. Eluates from autopolymerized resins yielded higher percentages of apoptosis and necrosis than the heat-polymerized ones. The results support our hypothesis that eluates of poly(methyl methacrylate)-based denture base acrylic resins activate death-signaling pathways, and that the extent of this process reflects their biocompatibility degree.
Cimpan
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MR
Institute of Odontology-Department of Dental Biomaterials, Faculty of Dentistry, University of Bergen, Norway. Mihaela-Roxana.Cimpan@cih.uib.no
Cressey
L I
LI
Skaug
N
N
Halstensen
A
A
Lie
S A
SA
Gjertsen
B T
BT
Matre
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R
D017304:Annexin A5; D017209:Apoptosis / Q000187:drug effects*; D001672:Biocompatible Materials / Q000633:toxicity; D002462:Cell Membrane / Q000187:drug effects; D053938:DNA Fragmentation; D003819:Denture Bases / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D016014:Linear Models; D008422:Materials Testing; D008854:Microscopy, Electron; D009336:Necrosis; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000633:toxicity*; D011419:Propidium; D020298:U937 Cells
D017304:Annexin A5; D001672:Biocompatible Materials; D011419:Propidium; D019904:Polymethyl Methacrylate