The Emp24 complex recruits a specific cargo molecule into endoplasmic reticulum-derived vesicles.
Members of the yeast p24 family, including Emp24p and Erv25p, form a heteromeric complex required for the efficient transport of selected proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. The specific functions and sites of action of this complex are unknown. We show that Emp24p is directly required for efficient packaging of a lumenal cargo protein, Gas1p, into ER-derived vesicles. Emp24p and Erv25p can be directly cross-linked to Gas1p in ER-derived vesicles. Gap1p, which was not affected by emp24 mutation, was not cross-linked. These results suggest that the Emp24 complex acts as a cargo receptor in vesicle biogenesis from the ER.
Muñiz
M
M
Biozentrum of the University of Basel, CH-4056 Basel, Switzerland.
Nuoffer
C
C
Hauri
H P
HP
Riezman
H
H
D000906:Antibodies / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational / Q000502:physiology; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D033921:Vesicular Transport Proteins
D000906:Antibodies; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; C093262:EMP24 protein, S cerevisiae; C102179:ERV25 protein, S cerevisiae; D005656:Fungal Proteins; C066709:GAS1 protein, S cerevisiae; D018840:HSP70 Heat-Shock Proteins; C060153:KAR2 protein, yeast; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D013439:Sulfhydryl Reagents; D033921:Vesicular Transport Proteins; D005033:Ethylmaleimide