title: Development and analysis of various clonal alloantigen-dependent cytotoxic cell lines from channel catfish.
abstract: To determine the phenotypes of cytotoxic cells in channel catfish, clonal alloantigen-dependent leukocyte lines were established from mixed leukocyte cultures. Each clone was analyzed for expression of TCR alpha and beta genes by RT-PCR and for target cell specificity by 51Cr-release assay. Based on the above criteria, the following five different cell types were identified among the 19 clones analyzed: 1) TCR alphabeta+ allospecific cytotoxic cells, 2) TCR alphabeta+ nonspecific cytotoxic cells, 3) allospecific TCR alphabeta+ noncytotoxic cells, 4) TCR alphabeta- nonspecific cytotoxic cells, and 5) TCR alphabeta- allospecific cytotoxic cells. The demonstration of cloned, TCR alphabeta+, allospecific cytotoxic effectors provides the strongest evidence to date for the existence of cytotoxic T cells in fish.
authors.affiliation: Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA.
authors.forename: T B
authors.initials: TB
authors.lastname: Stuge
authors.forename: M R
authors.initials: MR
authors.lastname: Wilson
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: K S
authors.initials: KS
authors.lastname: Barker
authors.forename: E
authors.initials: E
authors.lastname: Bengtén
authors.forename: G
authors.initials: G
authors.lastname: Chinchar
authors.forename: N W
authors.initials: NW
authors.lastname: Miller
authors.forename: L W
authors.initials: LW
authors.lastname: Clem
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation; D002454:Cell Differentiation / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells; D003601:Cytotoxicity Tests, Immunologic; D007059:Ictaluridae / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000302:isolation & purification; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology / Q000378:metabolism
chemical_list: D007519:Isoantigens; D016693:Receptors, Antigen, T-Cell, alpha-beta