title: Identification of unusual (modified and non-encoded) amino acid residues in peptides by combinations of high-performance liquid chromatography and high-performance capillary electrophoresis with matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
abstract: The techniques for micro-level analysis of some widespread unusual amino acids (phosphorylated and hydroxylated ones) as well as of some genetically non-encoded amino acids were developed for their subsequent identification in the peptide and protein amino acid sequence by narrow-bore column high-performance liquid chromatography (approximately 10 pmol of the sample), high-performance capillary electrophoresis (approximately 1-10 pmol), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (approximately 1-10 pmol), and automatic protein gas phase sequencing (approximately 1-50 pmol).
authors.affiliation: Analytical Protein Chemistry Department, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. arivanov@ibch.siobc.ras.ru
authors.forename: A R
authors.initials: AR
authors.lastname: Ivanov
authors.forename: I V
authors.initials: IV
authors.lastname: Nazimov
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000596:Amino Acids; D010455:Peptides