Chromate-induced human erythrocytes haemoglobin oxidation and peroxidation: influence of vitamin E, vitamin C, salicylate, deferoxamine, and N-ethylmaleimide.
In order to attenuate or to prevent chromate-induced human erythrocytes injury, the influence of vitamin E, vitamin C, salicylate, deferoxamine, and N-ethylmaleimide on chromate-induced human erythrocytes haemoglobin oxidation and peroxidation were investigated. It was observed that pretreatment of human erythrocytes with vitamin E (20 microM), vitamin C (1 mM), salicylate (3 mM), and deferoxamine (4 mM) significantly increased (P=0.0001) chromate-induced human erythrocytes haemoglobin oxidation in a time dependent manner, while it was significantly decreased (P=0.0001) by pretreatment with N-ethylmaleimide (1 mM). In contrast, pretreatment of human erythrocytes with deferoxamine (4 mM) immediately inhibited (P=0.0001) chromate-induced human erythrocytes peroxidation, while it was significantly increased (P=0.0001) by pretreatment with N-ethylmaleimide (1 mM) during the first 4 h of cells exposition to chromate. For time periods superior to 6 h pretreatment with N-ethylmaleimide (1 mM) significantly decreased (P=0.0001) chromate-induced human erythrocytes peroxidation. It was concluded that care must be taken as these drugs are used to prevent against toxicity induced by chromium(VI) compounds.
Fernandes
M A
MA
Departamento de Zoologia, Faculdade de Ciências, Universidade de Coimbra, 3004-517, Coimbra, Portugal.
Geraldes
C F
CF
Oliveira
C R
CR
Alpoim
M C
MC
D001205:Ascorbic Acid / Q000494:pharmacology; D002840:Chromates / Q000633:toxicity*; D003676:Deferoxamine / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology; D006454:Hemoglobins / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010084:Oxidation-Reduction / Q000187:drug effects; D012459:Salicylates / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors; D014810:Vitamin E / Q000494:pharmacology
D002840:Chromates; D006454:Hemoglobins; D012459:Salicylates; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; C016104:sodium bichromate; D003676:Deferoxamine; D005033:Ethylmaleimide; D001205:Ascorbic Acid