Bioanalytical applications of polyion-sensitive electrodes.
Recent developments in the design and bioanalytical applications of polyion-sensitive electrodes (PSEs) are reviewed. The general electrochemical principles governing the potentiometric response of such polymer membrane-based devices are summarized and new directions for the use of these novel sensors are detailed. These new directions include basic fundamental studies aimed at determining the thermodynamics of polyion extraction into ion exchanger-doped polymeric membranes, new methods to quantitate the anticoagulant drug heparin in whole blood via titrations with polycationic protamine, selective assays of protease activities (and inhibitors of such activities) using natural and synthetic polyionic peptides as substrates, and novel homogeneous immunoassay schemes based on potentiometric polyion detection.
Dai
S
S
Department of Chemistry, The University of Michigan, Ann Arbor 48109-1055, USA.
Esson
J M
JM
Lutze
O
O
Ramamurthy
N
N
Yang
V C
VC
Meyerhoff
M E
ME
D001695:Biology / Q000295:instrumentation*; D020811:Biomedical Technology; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004566:Electrodes