Closely similar values obtained when the ME7 strain of scrapie agent was titrated in parallel by two individuals in separate laboratories using two sublines of C57BL mice.
A single C57BL mouse-brain infected with the ME7 strain of mouse-passaged scrapie agent was used to carry out four parallel infectivity titrations in mice. These were carried out by two individuals in two laboratories using two sublines of C57BL mice. The titre values obtained by the four assays were very similar, and showed no significant differences between the two different operatives, the two different laboratories or the two different sublines of C57BL mice. The data confirm the validity of comparing these types of transmission data generated in different laboratories when a common methodology is used.
Taylor
D M
DM
Neuropathogenesis Unit, Institute for Animal Health, West Mains Road, Edinburgh, UK. david-m.taylor@bbsrc.ac.uk
McConnell
I
I
Ferguson
C E
CE
D000818:Animals; D001921:Brain / Q000473:pathology; D007753:Laboratories; D007928:Lethal Dose 50; D051379:Mice; D008810:Mice, Inbred C57BL; D015588:Observer Variation; D018620:PrPSc Proteins / Q000472:pathogenicity*; D017096:Prion Diseases / Q000473:pathology*; D014774:Virulence
D018620:PrPSc Proteins