title: Small heat shock proteins, IbpA and IbpB, are involved in resistances to heat and superoxide stresses in Escherichia coli.
abstract: To investigate the function of Escherichia coli small heat shock proteins, IbpA and IbpB, we constructed ibpA-, ibpB- and ibpAB-overexpressing strains and also an ibpAB-disrupted strain. The ibpA-, ibpB- and ibpAB-overexpressing strains were found to be resistant not only to heat but also to superoxide stress. However, the ibpAB-disrupted strain was not more sensitive to these stresses than the wild-type strain. The heat sensitivity of a rpoH amber mutant was partially suppressed by the overexpression of plac::ibpAB. These results suggest that IbpA and IbpB may be involved in the resistances to heat and oxidative stress.
authors.affiliation: Department of Biotechnology and High Technology Research Center, Kansai University, Yamate-cho 3-3-35, Suita, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumura
authors.forename: T
authors.initials: T
authors.lastname: Tsuchido
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D012808:Sigma Factor; D013481:Superoxides / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; C097578:IbpA protein, E coli; C097579:IbpB protein, E coli; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; D013481:Superoxides; D006861:Hydrogen Peroxide; D010269:Paraquat