Regulation of ATP-sensitive potassium channel mRNA expression in rat kidney following ischemic injury.
ATP-sensitive potassium (K(ATP)) channels are involved in the regulation of potassium homeostasis in kidneys. In the event of renal ischemia, they are thought to contribute to the important intracellular potassium loss observed in proximal tubules and thus to hypoxic injury. We have analyzed the transcriptional regulation of K(ATP) genes in rat kidney following transient renal ischemia. We observed that mRNA expression level was down-regulated for Kir1.1 and Kir4.1 potassium channels between 24 and 120 h after ischemia. In contrast, a strong increase in mRNA expression was observed for Kir6.1 shortly (2-6 h) after ischemia. Thus, renal ischemia followed by reperfusion provokes differential regulation of K(ATP) channel gene expression.
Sgard
F
F
Department of Molecular and Functional Genomics, Sanofi-Synthélabo, 10 Rue des Carrières, Rueil Malmaison, 92500, France.
Faure
C
C
Drieu la Rochelle
C
C
Graham
D
D
O'Connor
S E
SE
Janiak
P
P
Besnard
F
F
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D007511:Ischemia / Q000235:genetics*; D007668:Kidney / Q000098:blood supply / Q000378:metabolism*; D008297:Male; D015221:Potassium Channels / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley
D015221:Potassium Channels; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases