title: Crystallization and preliminary X-ray study of beta--mannosidase from Trichoderma reesei.
abstract: beta-Mannosidase from Trichoderma reesei, a 105 kDa glycoprotein, has been crystallized. The crystals belong to the space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell dimensions a = b = 165.86, c = 122.46 A, and diffract beyond 2.75 A resolution. X-ray diffraction data were collected from a frozen crystal on a synchrotron X-ray source.
authors.affiliation: National Synchrotron Light Laboratory, LNLS, Caixa Postal 6192, CEP 13083-970, Campinas, SP, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Aparicio
authors.forename: E V
authors.initials: EV
authors.lastname: Eneiskaya
authors.forename: A A
authors.initials: AA
authors.lastname: Kulminskaya
authors.forename: A N
authors.initials: AN
authors.lastname: Savel'ev
authors.forename: A M
authors.initials: AM
authors.lastname: Golubev
authors.forename: K N
authors.initials: KN
authors.lastname: Neustroev
authors.forename: J
authors.initials: J
authors.lastname: Kobarg
authors.forename: I
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008361:Mannosidases / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D011487:Protein Conformation; D014242:Trichoderma / Q000201:enzymology*; D044902:beta-Mannosidase
chemical_list: D005656:Fungal Proteins; D008361:Mannosidases; D044902:beta-Mannosidase