Use of the two-hybrid system to identify Rab-interacting proteins.
The yeast two-hybrid system has been useful for identifying many partners and effectors of small GTPases of the Rab family. We describe here such a screen using Rab6, a protein involved in the regulation of intracellular transport at the level of the Golgi apparatus, as bait.
Janoueix-Lerosey
I
I
INSERM U 509, Laboratoire Mécanismes moléculaires du transport intracellulaire, UMR CNRS 144, Institut Curie, 26 rue d'Ulm, Paris Cedex 05, 75248, France.
Goud
B
B
D020730:Guanine Nucleotide Dissociation Inhibitors / Q000235:genetics / Q000302:isolation & purification*; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification; D020798:Two-Hybrid System Techniques; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
D020730:Guanine Nucleotide Dissociation Inhibitors; D020033:Protein Isoforms; C094135:Rab6 protein; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D020691:rab GTP-Binding Proteins