title: The reduction of the Rieske iron-sulfur cluster in naphthalene dioxygenase by X-rays.
abstract: Naphthalene 1,2 dioxygenase (NDO) displays characteristic UV-Vis spectra depending on the oxidation state of the Rieske center. Investigations on crystals of NDO grown for X-ray diffraction experiments showed spectra characteristic of the oxidized form. Crystals reduced in an anaerobic glovebox using sodium-dithionite showed a characteristic reduced spectrum. Spectra of crystals (cooled to 100 K) after being exposed to X-rays for data collection showed spectra corresponding to a reduced Rieske iron center, demonstrating the ability of X-rays to change the oxidation state of the Rieske iron-sulfur cluster in NDO.
authors.affiliation: Department of Molecular Biology, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala.
authors.forename: A
authors.initials: A
authors.lastname: Karlsson
authors.forename: J V
authors.initials: JV
authors.lastname: Parales
authors.forename: R E
authors.initials: RE
authors.lastname: Parales
authors.forename: D T
authors.initials: DT
authors.lastname: Gibson
authors.forename: H
authors.initials: H
authors.lastname: Eklund
authors.forename: S
authors.initials: S
authors.lastname: Ramaswamy
mesh_terms: D003460:Crystallization; D049308:Dioxygenases; D004926:Escherichia coli / Q000201:enzymology; D007501:Iron / Q000378:metabolism / Q000528:radiation effects*; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D015591:Microspectrophotometry; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D013455:Sulfur / Q000378:metabolism / Q000528:radiation effects*; D013696:Temperature; D013997:Time Factors; D014965:X-Rays
chemical_list: D007506:Iron-Sulfur Proteins; D009097:Multienzyme Complexes; D013455:Sulfur; D007501:Iron; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase