Cloning and expression of nope, a new mouse gene of the immunoglobulin superfamily related to guidance receptors.
The novel mouse gene Nope was identified due to its proximity to the Punc gene on chromosome 9. With a domain structure of four immunoglobulin domains, five fibronectin type III repeats, a single transmembrane domain, and a cytoplasmic domain, Nope encodes a new member of the immunoglobulin superfamily of cell surface proteins. It displays a high level of similarity to Punc, as well as to guidance receptors such as the Deleted in Colorectal Cancer protein and Neogenin. Nope is expressed during embryonic development in the notochord, in developing skeletal muscles, and later in the ventricular zone of the nervous system. In the adult brain, Nope can be detected in the hippocampus. Radiation hybrid mapping of Nope, Punc, and Neogenin placed all three genes in close vicinity on mouse chromosome 9.
Salbaum
J M
JM
The Neurosciences Institute, 10640 John J. Hopkins Drive, San Diego, CA 92121, USA. msalbaum@unmc.edu
Kappen
C
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms; D000075389:DCC Receptor; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D017434:Protein Structure, Tertiary; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D025521:Tumor Suppressor Proteins
D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D000075389:DCC Receptor; C501909:DCC protein, human; D018076:DNA, Complementary; C501910:Dcc protein, mouse; D007136:Immunoglobulins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C408892:Nope protein, mouse; C112440:Punc protein, mouse; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; C091279:neogenin