ARF1 regulates pH-dependent COP functions in the early endocytic pathway.
Coat proteins of the COP family were recently shown by us and others to be involved in membrane transport in the endocytic pathway, in addition to their known functions in the biosynthetic pathway. We have also shown that membrane association of endosomal COPs depends on the acidic endosomal pH, in contrast to biosynthetic COPs. In this paper, we report that both membrane recruitment of endosomal COPs and in vitro biogenesis of transport intermediates destined for late endosomes, depend on a cytosolic factor, which we identified as the small GTP-binding protein ARF1. Our data indicate that ARF1 does not act via activation of an endosomal phospholipase D. We also find that ARF1 membrane association is regulated by the endosomal pH, and that this controls the pH-dependent association of endosomal COPs. These studies thus show that ARF1 regulates COP functions in the endocytic pathway, and indicate that ARF1 acts as the cytosolic component of a transmembrane pH-sensing mechanism.
Gu
F
F
Department of Biochemistry, Sciences II, University of Geneva, 30 quai E. Ansermet, CH-1211 Geneva 4, Switzerland.
Gruenberg
J
J
D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism*; D000818:Animals; D020756:Coatomer Protein / Q000378:metabolism*; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D011485:Protein Binding
D020756:Coatomer Protein; D010712:Phosphatidic Acids; D010739:Phospholipase D; D020823:ADP-Ribosylation Factor 1