Chromatographic aspects in high throughput liquid chromatography/mass spectrometry.
Important parameters to consider when developing a liquid chromatography/mass spectrometry (LC/MS) method are buffer type and concentration, and column geometry. In the work presented here the choice of buffer for the analysis of basic compounds using a polar embedded phase (HyPURITYtrade mark ADVANCE) is illustrated for the analysis of tricyclic antidepressants. Method transfer from a 4.6 mm i.d. column to a 2.1 mm i.d. column is demonstrated for the analysis of triazines and anabolic steroids and their metabolites, with no change in selectivity and with added speed of analysis. Analysis of eight beta-blockers is achieved in 65 seconds by using a short 30 x 4.6 mm C18 column.
Pereira
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L
ThermoQuest Chromatography Supplies, Hypersil Division, 112 Chadwick Road, Astmoor, Runcorn, Cheshire WA7 1PR, UK.
Ross
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P
Woodruff
M
M
D000319:Adrenergic beta-Antagonists / Q000737:chemistry*; D000929:Antidepressive Agents, Tricyclic / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D013256:Steroids / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry*
D000319:Adrenergic beta-Antagonists; D000929:Antidepressive Agents, Tricyclic; D013256:Steroids; D014227:Triazines