Cultured tumor cells of murine submandibular gland origin: a model to investigate pHi regulation of salivary cells.
Intracellular pH (pHi) and several ion transport mechanisms in cultured murine salivary gland cells (SCA-9) were studied using a videomicrofluorometric method and the H+-specific probe C-SNARF-1. The aim of this study was to test SCA-9 cells' pHi regulation mechanisms and evaluate if this cell line is representative of submandibular gland cells. Resting pHi in unstimulated cells was estimated to be 7.17+/-0.07. To investigate the presence of Na+/H+ and Cl-/HCO3- antiports as well as Na+/K+/2Cl- symports in SCA-9 cells, we used different specific blockers, dimethyl-amiloride, disulfonic stilbene, bumetanide and furosemide. In order to study SCA-9 cell capacity to regulate their pHi in response to alkaline and acid loads, we applied the NH4Cl prepulse method to all these blockers. The results showed that SCA-9 cells possess both antiports and symports involved in pHi regulation, and that this cell line can be used as a convenient model to study pHi regulatory mechanisms in salivary cells.
Trzaskawka
E
E
Faculty of Odontology, Laboratory of Physiology, University of Montpellier I, France. trzaskaw@univ-perp.fr
Vigo
J
J
Egea
J C
JC
Goldsmith
M C
MC
Salmon
J M
JM
De Periere
D D
DD
D000136:Acid-Base Equilibrium; D000643:Ammonium Chloride; D000818:Animals; D017920:Antiporters / Q000037:antagonists & inhibitors / Q000378:metabolism; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D027963:Chloride-Bicarbonate Antiporters; D005470:Fluorometry; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D017136:Ion Transport; D051379:Mice; D018715:Microscopy, Video; D008954:Models, Biological; D017923:Sodium-Hydrogen Exchangers / Q000037:antagonists & inhibitors / Q000378:metabolism; D028021:Sodium-Potassium-Chloride Symporters; D013363:Submandibular Gland / Q000166:cytology* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D017920:Antiporters; D002352:Carrier Proteins; D027963:Chloride-Bicarbonate Antiporters; D017923:Sodium-Hydrogen Exchangers; D028021:Sodium-Potassium-Chloride Symporters; D000643:Ammonium Chloride; D006859:Hydrogen