Comparative genomic hybridization and conventional cytogenetic analyses in childhood acute myeloid leukemia.
Comparative genomic hybridization (CGH) analysis was performed on bone marrow specimens from 19 children with acute myeloid leukemia (AML) at diagnosis. The results of CGH were compared to those of conventional cytogenetic analysis. The most common CGH aberrations were gains of whole chromosomes 6 and 8, both of which appeared three times. Two losses were seen twice; losses of whole chromosomes 7 and X. The CGH findings were concordant with the results of conventional karyotyping. CGH did not add new information to the karyotypes. Since no high-level amplification was found among the samples and standard karyotyping was highly successful, we do not advocate routine use of CGH in the diagnostic evaluation of childhood AML.
Huhta
T
T
Department of Medical Genetics, Haartman Institute, University of Helsinki, Finland.
Vettenranta
K
K
Heinonen
K
K
Kanerva
J
J
Larramendy
M L
ML
Mahlamäki
E
E
Saarinen-Pihkala
U M
UM
Knuutila
S
S
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D020732:Cytogenetic Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm