Evaluation of bone tissue on metallic implants by energy-dispersive x-ray analysis: an experimental study.
Osseointegration capacity of the different metallic implants depends on several variables. Osseointegration can be evaluated by using different methodologies, such as light microscopy and scanning or transmission electron microscopy. The aim of this study was to develop a qualitative and quantitative method to evaluate the presence of bone tissue on large metallic surfaces. A laminar implant was placed in each tibia of 10 Wistar rats. The animals were killed 30 days after surgery. Tibiae were resected, one for embedding in methyl methacrylate and the other for evaluation by energy-dispersive x-ray analysis. Light microscopy revealed osseointegration. Observation of the implant surface by scanning electron microscopy revealed the presence of a coating on the metallic surface that was rough in some areas and smooth in others. Analysis of the coating by energy-dispersive x-ray analysis showed the presence of Ca and P. Eighty percent (+/- 10%) of the metallic implant surface exhibited bone tissue. After confirmation of the occurrence of osseointegration capacity using light microscopy, the method described here allows qualitative and quantitative evaluation of the bone tissue found on large metallic surfaces.
Guglielmotti
M B
MB
Department of Oral Pathology, Faculty of Dentistry, University of Buenos Aires, Argentina. info@odon.uba.ar
Renou
S
S
Cabrini
R L
RL
D000818:Animals; D001842:Bone and Bones / Q000737:chemistry / Q000648:ultrastructure; D002118:Calcium / Q000032:analysis; D003758:Dental Implantation, Endosseous; D004577:Electron Probe Microanalysis; D020007:Implants, Experimental; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D016348:Osseointegration; D010758:Phosphorus / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia; D014025:Titanium
D010758:Phosphorus; D014025:Titanium; D002118:Calcium