Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin.
O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.
Kisiel
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Department of General and Organic Chemistry, Institute of Chemistry, Medical Academy of Białystok, Poland.
Gindzieński
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Gadek
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D000180:Acrylic Resins; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005752:Gastric Mucins / Q000737:chemistry*; D006834:Hydrazines; D066298:In Vitro Techniques; D009844:Oligosaccharides / Q000302:isolation & purification; D011134:Polysaccharides / Q000302:isolation & purification*; D012996:Solutions; D013552:Swine; D014867:Water
D000180:Acrylic Resins; D005752:Gastric Mucins; D006834:Hydrazines; D009844:Oligosaccharides; D011134:Polysaccharides; D012996:Solutions; D014867:Water; C029424:hydrazine; C046089:Bio-Gel P-4