Mechanism of DNA hydrodynamic separation in chromatography.
An alternative chromatographic procedure for the separation of large double-stranded DNA molecules was discovered recently and called "slalom chromatography". This fractionation is based on a new hydrodynamic process that is determined by the progression of the mobile-phase flow through the interstitial spaces created between the highly packed particles inside the column. Here, the separation is treated as the result of a slowing down of the large double-stranded DNA fragments in relation to their size with the flow direction changing around the particles. A model, based on the concept derived from the reorientation time of macromolecules, was adequate to describe the hydrodynamic phenomenon. This model constitutes an attractive tool to enhance the expansion of this chromatographic procedure and provide valuable information on the dynamic behavior of biological polymers.
Peyrin
E
E
Laboratoire de Chimie Analytique, UFR de Pharmacie, La Tronche, France.
Guillaume
Y C
YC
Villet
A
A
Favier
A
A
D010582:Bacteriophage lambda / Q000737:chemistry; D005591:Chemical Fractionation / Q000379:methods; D002845:Chromatography / Q000379:methods*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification*; D008956:Models, Chemical; D008970:Molecular Weight
D004279:DNA, Viral