Intrinsic regulation of differentiation markers in human epidermis, hard palate and buccal mucosa.
Different epithelia show extensive variation in differentiation. Epidermis and epithelium from the hard palate are both typical examples of orthokeratinized epithelia whereas buccal mucosa is an example of a non-keratinized epithelium. Each of these tissues can be distinguished morphologically and also by the expression of a number of structural proteins. Tissue explants derived from epidermis, hard palate or buccal mucosa were cultured at the air-liquid interface on collagen gels containing human dermal fibroblasts. Reconstructed epithelia that retained many of the morphological and immunohistochemical characteristics of the original tissue were formed. Cultures derived from epidermis and the hard palate both had a well-defined stratum basale, stratum spinosum, stratum granulosum and stratum corneum whereas cultures derived from buccal mucosa had no stratum granulosum or corneum and the cells retained their nuclei. Significantly more living cell layers were observed in both types of epithelia obtained from the mouth than in epidermis. The specific localization of proliferation and differentiation markers (Ki67, loricrin, involucrin, SPRR2, SPRR3 and keratin 10) closely resembled that of the tissue from which the cultures were derived. As identical three-dimensional culture models were used here, it is concluded that the differences observed between these epithelia were due to intrinsic properties of the keratinocytes.
Gibbs
S
S
Department of Dermatology, Leiden University Medical Centre, Sylvius Laboratory, The Netherlands. gibbs@rullf2.medfac.leidenuniv.nl
Ponec
M
M
D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003094:Collagen; D055233:Cornified Envelope Proline-Rich Proteins; D003470:Culture Media; D000078404:Epidermal Cells; D004847:Epithelial Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D005782:Gels; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D015603:Keratinocytes / Q000166:cytology; D007633:Keratins / Q000032:analysis; D019394:Ki-67 Antigen / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D009061:Mouth Mucosa / Q000166:cytology*; D010159:Palate / Q000166:cytology*; D010455:Peptides; D055232:Proline-Rich Protein Domains; D011498:Protein Precursors / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012867:Skin / Q000166:cytology
D015415:Biomarkers; D055233:Cornified Envelope Proline-Rich Proteins; D003470:Culture Media; D005782:Gels; D007381:Intermediate Filament Proteins; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; C491986:SPRR2B protein, human; C491987:SPRR2D protein, human; C112254:SPRR3 protein, human; C064554:loricrin; C032253:involucrin; D007633:Keratins; D003094:Collagen