[Effects of MMP-1 expressing plasmid on rat liver fibrosis].
OBJECTIVE
To observe the effects of MMP-1(Matrix Metalloproteinase-1) expressing plasmid on rat liver fibrosis.


METHODS
We constructed the rat's MMP-1 recombinant plasmid which can be expressed in eucaryotic cells. After encapsulating the MMP-1 recombinant plasmid by lipofectin, we transferred it to the rat's fibrotic liver in vivo intraperitoneally, then observed the effects of the plasmid on the fibrotic liver by RT-PCR, immunohistochemistry and the observation of pathology.


RESULTS
The recombinant plasmid of MMP-1 could increase the degradation of type I and type III collagen in the rat's fibrotic liver according to immunohistochemistry compared with fibrotic modal group(P < 0.01) or compared with colchicine group(P < 0.05). According to the observation of pathology, recombinant plasmid of MMP-1 has some reverse effects on the rat's fibrotic liver compared with fibrotic model group(P < 0.05), but no obvious difference was found between the plasmid and colchicine(P > 0.05).


CONCLUSIONS
The recombinant plasmid of MMP-1 has some reverse effects on liver fibrosis, but the effects is limited.
Yang
C
C
Institute of Infectious Disease, Xiangya Hospital, Hunan Medical University, Changsha.
Hu
G
G
Tan
D
D
D000818:Animals; D003094:Collagen / Q000378:metabolism; D007150:Immunohistochemistry; D008099:Liver / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D010957:Plasmids; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003094:Collagen; D020781:Matrix Metalloproteinase 1