Dielectric resonator-based side-access probe for muscle fiber EPR study.
We present a novel dielectric resonator (DR)-based resonant structure that accommodates aqueous sample capillaries in orientations that are either parallel (i.e., side-access) or perpendicular to the direction of an external (Zeeman) magnetic field, B(0). The resonant structure consists of two commercially available X-band DRs that are separated by a Rexolite spacer and resonate in the fundamental TE(01delta) mode. The separator between the DRs is used to tune the resonator to the desired frequency and, by appropriately drilled sample holes, to provide access for longitudinal samples, notably capillaries containing oriented, spin-labeled muscle fibers. In contrast to the topologically similar cylindrical TE(011) cavity, the DR-based structure has distinct microwave properties that favor its use for parallel orientation of lossy aqueous samples. For perpendicular orientation of a dilute (6.25 microM) aqueous solution of IASL spin label, the S/N ratio was at least one order of magnitude better for the side-access DR-based structure than for a standard TE(102) cavity. EPR spectra acquired for maleimide spin-labeled myosin filaments also revealed ca. 10 times better S/N ratio than those obtained with a standard TE(102) cavity. For the side-access DR with sample capillaries oriented either parallel or perpendicular to the external magnetic field, the Q- and filling factors are in good agreement with the theoretical estimates derived from the distribution of magnetic (H(1)) and electric (E(1)) components.
Sienkiewicz
A
A
Institute of Physics, Polish Academy of Sciences, Al. Lotnikow 32/46, Warsaw, 02-668, Poland.
Jaworski
M
M
Smith
B G
BG
Fajer
P G
PG
Scholes
C P
CP
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods; D066298:In Vitro Techniques; D018485:Muscle Fibers, Skeletal / Q000737:chemistry*; D011817:Rabbits