Peptide YY stimulates the expression of apolipoprotein A-IV gene in Caco-2 intestinal cells.
The effect of peptide YY, a gastrointestinal hormone, on the expression of the apolipoprotein A-IV gene in the intestinal epithelial cell line Caco-2 was examined by semiquantitative RT-PCR followed by Southern hybridization with an inner oligonucleotide probe. Apolipoprotein A-IV mRNA levels were increased in response to peptide YY in a dose- and time-dependent fashion. Western blotting revealed that the exogenous peptide YY increased the intracellular concentration of apolipoprotein A-IV. In contrast, apolipoprotein A-I, B, and C-III mRNA did not respond to peptide YY. Differentiated Caco-2 cells expressed Y1- but not Y2- and Y5-receptor subtype mRNA. The present results suggest that peptide YY modulates apolipoprotein A-IV gene expression, likely via the Y1-receptor subtype in intestinal epithelial cells.
Sonoyama
K
K
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan. ksnym@chem.agr.hokudai.ac.jp
Suzuki
K
K
Kasai
T
T
D001054:Apolipoproteins A / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D018938:Caco-2 Cells; D005786:Gene Expression Regulation; D006801:Humans; D007422:Intestines / Q000166:cytology; D008074:Lipoproteins / Q000378:metabolism; D019894:Peptide YY / Q000502:physiology*
D001054:Apolipoproteins A; D008074:Lipoproteins; C022205:apolipoprotein A-IV; D019894:Peptide YY