title: Up-regulation of sigma(1) receptor mRNA in rat brain by a putative atypical antipsychotic and sigma receptor ligand.
abstract: Sigma(1) (sigma(1)) receptor mRNA expression was studied in the prefrontal cortex, striatum, hippocampus and cerebellum of rat brain by northern blot and in situ hybridization. The effects of a chronic treatment with antipsychotic drugs (haloperidol and clozapine), and with E-5842, a sigma(1) receptor ligand and putative atypical antipsychotic on sigma(1) receptor expression were examined. A significant increase in the levels of sigma(1) receptor mRNA in the prefrontal cortex and striatum after E-5842 administration was observed, while no apparent changes were seen with either haloperidol or clozapine. Our results suggest a long-term adaptation of the sigma(1) receptor at the level of mRNA expression in specific areas of the brain as a response to a sustained treatment with E-5842.
authors.affiliation: Department of Neuropharmacology, Research Center, Laboratorio Esteve S.A., Verge de Montserrat 221, 08041, Barcelona, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Zamanillo
authors.forename: F
authors.initials: F
authors.lastname: Andreu
authors.forename: S
authors.initials: S
authors.lastname: Ovalle
authors.forename: M P
authors.initials: MP
authors.lastname: Pérez
authors.forename: G
authors.initials: G
authors.lastname: Romero
authors.forename: A J
authors.initials: AJ
authors.lastname: Farré
authors.forename: X
authors.initials: X
authors.lastname: Guitart
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D017403:In Situ Hybridization; D008024:Ligands; D008297:Male; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017480:Receptors, sigma / Q000378:metabolism*; D014230:Triazoles / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D014150:Antipsychotic Agents; C117757:E 5842; D008024:Ligands; D011725:Pyridines; D012333:RNA, Messenger; D017480:Receptors, sigma; D014230:Triazoles; C101491:sigma1-binding protein, rat