Alterations in the properties and isoforms of sciatic nerve Na(+), K(+)-ATPase in methylcyclopentadienyl manganese tricarbonyl-treated mice.
The in vivo effect of methylcyclopentadienyl manganese tricarbonyl (MMT), an organic manganese-containing compound, on the mouse motor nerve was studied. The motor nerve conduction velocity was markedly decreased in MMT-treated mice. The Na(+),K(+)-ATPase activity of sciatic nerve isolated from MMT-treated mice was decreased; however, the sciatic nerve Na(+),K(+)-ATPase activity was not affected by the in vitro treatment of MMT. Moreover, [(3)H]ouabain binding of sciatic nerve isolated from MMT-treated mice was decreased. Using Western blot analysis, the amount of Na(+),K(+)-ATPase catalytic alpha1 subunit polypeptide in sciatic nerve of MMT-treated mice was also decreased. These results indicate that a causal relationship may exist between reduced nerve Na(+),K(+)-ATPase activity and motor nerve conduction velocity in MMT-treated mice and that a measurable decrease in alpha1 catalytic subunit isoform of Na(+),K(+)-ATPase may be necessary for the development of peripheral neuropathy by MMT.
Liu
S H
SH
Institute of Toxicology, National Taiwan University, Taipei, 10043, Taiwan.
Wang
J H
JH
Kang
J J
JJ
Lin
R H
RH
Lin-Shiau
S Y
SY
D000818:Animals; D007527:Isoenzymes; D051379:Mice; D009431:Neural Conduction / Q000187:drug effects*; D009942:Organometallic Compounds / Q000009:adverse effects*; D010042:Ouabain; D012584:Sciatic Nerve / Q000187:drug effects / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D007527:Isoenzymes; D009942:Organometallic Compounds; C009907:2-methylcyclopentadienyl manganese tricarbonyl; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase