title: Understanding ion transport by quantification of mitochondrial swelling.
abstract: Suspensions of mitochondria are turbid and scatter light. An increase in the matrix volume (swelling) due to the influx of permeable solutes results in a decrease in the amount of light scattered. This property can be used to study solute fluxes across the mitochondrial inner membrane. A rapid method for isolating mitochondria is presented along with three swelling experiments using energized and non-energized mitochondria to illustrate ion transport across energy transducing membranes.
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana, Iztapalapa, Av. Michoacán y La Purísima, 09340, México, Mexico
authors.initials: M
authors.lastname: Königsberg