Preparation and characterization of polyclonal antibodies against human chaperonin 10.
Early pregnancy factor (EPF) has been identified as an extracellular homologue of chaperonin 10 (Cpn10), a heat shock protein that functions within the cell as a molecular chaperone. Here, we report the production of polyclonal antibodies directed against several different regions of the human Cpn10 molecule and their application to specific protein quantitation and localization techniques. These antibodies will be valuable tools in further studies to elucidate the mechanisms underlying the differential spatial and temporal localization of EPF and Cpn10 and in studies to elucidate structure and function.
Somodevilla-Torres
M J
MJ
Department of Surgery, University of Queensland, Brisbane, Australia.
Hillyard
N C
NC
Morton
H
H
Alewood
D
D
Halliday
J A
JA
Alewood
P F
PF
Vesey
D A
DA
Walsh
M D
MD
Cavanagh
A C
AC
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002277:Carcinoma / Q000737:chemistry; D018835:Chaperonin 10 / Q000032:analysis / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis; D010446:Peptide Fragments / Q000276:immunology; D011233:Precipitin Tests; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology
D000906:Antibodies; D018835:Chaperonin 10; D007106:Immune Sera; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins