title: HLA class II alleles associations of anticardiolipin and anti-beta2GPI antibodies in a large series of European patients with systemic lupus erythematosus.
abstract: The objective of this study was to determine the HLA class II associations of the anticardiolipin (aCL) and anti-beta2GPI (abeta2GPI) antibodies in a large series of European patients with systemic lupus erythematosus (SLE). A cohort of 577 European SLE patients was enrolled. aCL and abeta2GPI were measured by ELISA methods. Molecular typing of HLA-DRB1, DRB3, DRB4, DRB5, DQA1 and DQB1 loci was performed by the polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) method. aCL of IgG, IgM and IgA isotypes were detected in 22.8%, 14% and 13.9% of patients, respectively. IgG and IgM abeta2GPI were detected in 20% of patients. aCL showed positive association with HLA DRB1*04, DRB1*0402, DRB1*0403, DRB1*07, DRB3*0301, DQA1*0201, DQA1*0301, DQB1*0302, and negative association with DQA1*0501, DRB3*0202. abeta2GPI showed positive association with DRB1*0402, DRB1*0403, DQB1*0302. DRB1*0402 carried the highest relative risk for the presence of both aCL (RR=8. 1) and abeta2GPI (RR=4.6). Our results confirm the already described associations of aCL with HLA DR4 and DR7, but also demonstrate that, among the alleles at the DRB1*04 locus, the *0402 was most represented both in aCL and in abeta2GPI positive patients. In addition, HLA class II associations of abeta2GPI are for the first time extensively examined in a large cohort of European SLE patients.
authors.affiliation: Istituto di Reumatologia, Università di Siena, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Galeazzi
authors.forename: G D
authors.initials: GD
authors.lastname: Sebastiani
authors.forename: A
authors.initials: A
authors.lastname: Tincani
authors.forename: J C
authors.initials: JC
authors.lastname: Piette
authors.forename: F
authors.initials: F
authors.lastname: Allegri
authors.forename: G
authors.initials: G
authors.lastname: Morozzi
authors.forename: F
authors.initials: F
authors.lastname: Bellisai
authors.forename: R
authors.initials: R
authors.lastname: Scorza
authors.forename: G B
authors.initials: GB
authors.lastname: Ferrara
authors.forename: C
authors.initials: C
authors.lastname: Carcassi
authors.forename: J
authors.initials: J
authors.lastname: Font
authors.forename: G
authors.initials: G
authors.lastname: Passiu
authors.forename: J
authors.initials: J
authors.lastname: Smolen
authors.forename: C
authors.initials: C
authors.lastname: Papasteriades
authors.forename: F
authors.initials: F
authors.lastname: Houssiau
authors.forename: A F
authors.initials: AF
authors.lastname: Nebro
authors.forename: E D
authors.initials: ED
authors.lastname: Ramon Garrido
authors.forename: A
authors.initials: A
authors.lastname: Jedryka-Goral
authors.forename: R
authors.initials: R
authors.lastname: Marcolongo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D017153:Antibodies, Anticardiolipin / Q000276:immunology*; D004247:DNA / Q000032:analysis; D015342:DNA Probes / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005060:Europe; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D053482:beta 2-Glycoprotein I
chemical_list: D017153:Antibodies, Anticardiolipin; D015342:DNA Probes; D006023:Glycoproteins; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D007132:Immunoglobulin Isotypes; D053482:beta 2-Glycoprotein I; D004247:DNA