Expression of cholesteryl glucoside by heat shock in human fibroblasts.
We investigated the heat-induced alteration of glycolipids in human cultured cells, TIG-3 fibroblasts, to show the expression of steryl glucoside by heat shock. A glycolipid band was detected on a thin-layer chromatography plate in lipid extracts from TIG-3 cells exposed to high temperature (42 degrees C) for 15 and 30 minutes, while it was hardly detectable without heat shock. Both cholesterol and glucose were almost exclusively detected by gas liquid chromatography as degradation products of the lipid. The structure of the lipid molecule was elucidated by electrospray mass spectrometry to be a cholesteryl glucoside. This is the first report to show the occurrence of a steryl glucoside in mammalian cells, and this substance is considered to have a significant role in heat shock responses in mammalian cells.
Kunimoto
S
S
Graduate School of Human Environmental Science, Department of Advanced Biosciences, Faculty of Science, Ochanomizu University, Tokyo, Japan.
Kobayashi
T
T
Kobayashi
S
S
Murakami-Murofushi
K
K
D002478:Cells, Cultured; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism; D006017:Glycolipids / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D013058:Mass Spectrometry; D013312:Stress, Physiological / Q000378:metabolism
D006017:Glycolipids; C027578:cholesteryl glucoside; D002784:Cholesterol; D005964:Glucosyltransferases; C023256:UDPglucose-sterol glucosyltransferase