Comparison between micellar electrokinetic chromatography and HPLC for the determination of Betamethasone Dipropionate, Clotrimazole and their related substances.
The complete separation of a composite mixture that consisted of Betamethasone Dipropionate (BMD), Clotrimazole and their derivatives in a pharmaceutical dosage form was achieved within 15 min using sodium dodecyl sulfate (SDS) micellar electrokinetic chromatography (MEKC). For the MEKC separations, electrophoretic media consisting of SDS-phosphate buffer and various concentrations of alcohols or acetonitrile were used. The optimal condition for separating BMD, Clotrimazole and their analogues was found to be 50 mM SDS-15% acetonitrile-5% butanol at pH 7.2. The results demonstrated that the method was valid for the quantitation of BMD, Clotrimazole and analogues with selectivity and precision comparable to that of High-Performance Liquid Chromatography (HPLC).
Lin
M
M
Department of Chemistry, State University of New York at Birmingham, 13902-6000, USA.
Wu
N
N
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000302:isolation & purification; D000935:Antifungal Agents / Q000032:analysis / Q000302:isolation & purification; D001623:Betamethasone / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003022:Clotrimazole / Q000032:analysis* / Q000302:isolation & purification; D005240:Feasibility Studies; D005938:Glucocorticoids; D011786:Quality Control; D015203:Reproducibility of Results
D000893:Anti-Inflammatory Agents; D000935:Antifungal Agents; D005938:Glucocorticoids; C011175:betamethasone-17,21-dipropionate; D001623:Betamethasone; D003022:Clotrimazole