Enhanced apoptosis in retrovirally transfected osteosarcoma cells after exposure to sodium butyrate.
We investigated the effects of sodium butyrate (NaBu) on two retrovirally transfected osteosarcoma cell lines (1547-TK and 1547-LacZ cells) compared to the corresponding untransfected cell line. The first finding was an inhibitory effect only on the proliferation of both transfected cell lines. This antiproliferative effect was associated with apoptosis induction, which was detected using techniques that monitor either characteristic biochemical or morphological processes. Our findings show that 1547-TK and 1547-LacZ cells were much more sensitive to NaBu treatment than untransfected 1547 cells as concerns both proliferation and apoptosis induction.
Moalic
S
S
Faculté de Pharmacie, 87025 Limoges Cedex, France.
Liagre
B
B
Labrousse
F
F
Beneytout
J L
JL
D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000473:pathology*; D002087:Butyrates / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000473:pathology; D004249:DNA Damage; D053938:DNA Fragmentation; D006801:Humans; D012516:Osteosarcoma / Q000473:pathology*; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D002087:Butyrates