title: Characterization of an avidin-bonded column for direct injection in reversed-phase high-performance liquid chromatography.
abstract: A denatured avidin-bonded column was suitable for use in the reversed-phase HPLC and complete recovery of serum protein over a wide range of pH. Retention property of the denatured avidin-bonded column was very nearly to a non-denatured avidin-bonded column already reported and, however, showed very high stability to organic solvent. A performance of the denatured avidin-bonded column was maintained even if 500 continuous injections of human serum (total 10 ml).
authors.affiliation: GL Sciences, Saitama, Japan. rd@gls.co.jp
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Ohira
mesh_terms: D001360:Avidin / Q000737:chemistry*; D001769:Blood; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration
chemical_list: D002021:Buffers; D001360:Avidin