title: NMR measurements of proton exchange between solvent and peptides and proteins.
abstract: Scope and limitations of the NMR based methods, equilibration and magnetization transfer, for measuring proton exchange rates of amide protons in peptides and proteins with water protons are discussed. Equilibration is applied to very slow processes detected by hydrogen-deuterium exchange after a solute is dissolved in D2O. Magnetization transfer allows to study moderately rapid processes in H2O. A number of precautions should be undertaken in order to avoid systemic errors inherent in the magnetization transfer method.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.
authors.forename: J
authors.initials: J
authors.lastname: Wójcik
authors.forename: K
authors.initials: K
authors.lastname: Ruszczyńska
authors.forename: I
authors.initials: I
authors.lastname: Zhukov
authors.forename: A
authors.initials: A
authors.lastname: Ejchart
mesh_terms: D000595:Amino Acid Sequence; D017666:Deuterium Oxide; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011522:Protons; D012997:Solvents; D014867:Water
chemical_list: D010455:Peptides; D011506:Proteins; D011522:Protons; D012997:Solvents; D014867:Water; D017666:Deuterium Oxide