title: Dimorphic features of the different alpha-containing GABA-A receptor subtypes in the cortico-basal ganglia system of two distantly related mammals (hedgehog and rat)
abstract: This investigation represents a first study dealing with the dimorphic differences of the main alpha-containing gamma-aminobutyric acid (GABA(A)) receptors in the brain of two distantly related mammals (hedgehog and rat). The labeling of these receptors in the presence of zolpidem (highly selective benzodiazepine agonist) and under the different degree of GABA(A)/benzodiazepine allosteric coupling activities accounted for a heterogeneous colocalization of alpha1-enriched and of alpha2/3-enriched and alpha5-enriched GABA(A) receptors in some areas of the cortico-basal ganglia system (including the important ventrolateral thalamic station) of both mammalian sexes. In the hedgehog, the greatest (P<0.001) GABA-dependent reduction of zolpidem inhibition constants was mostly registered in alpha1- and/or alpha5-enriched areas, such as the frontoparietal cortex lamina III (235%), ventrolateral thalamic nucleus (128%), and substantia nigra pars reticulata (110%) of the male. However, the greatest reductions in the rat were instead detected in the male substantia nigra pars reticulata (192%) and female striatum (120%), areas which are enriched either by the colocalization of alpha1- with alpha2/3-subunits or by all three alpha-subunits. These results support the contention that a sex-related alpha-containing GABA(A) receptor sensitivity constitutes an important element in the execution of skilled motor activities during the different socio-sexual behaviors of the two mammals.
authors.affiliation: Ecology Department, University of Calabria, Cosenza, Italy.
authors.forename: R M
authors.initials: RM
authors.lastname: Facciolo
authors.forename: R
authors.initials: R
authors.lastname: Alo'
authors.forename: R
authors.initials: R
authors.lastname: Tavolaro
authors.forename: M
authors.initials: M
authors.lastname: Canonaco
authors.forename: M F
authors.initials: MF
authors.lastname: Franzoni
mesh_terms: D000818:Animals; D001345:Autoradiography; D001479:Basal Ganglia / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D005260:Female; D005445:Flunitrazepam / Q000493:pharmacokinetics; D006364:Hedgehogs; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D010296:Parietal Lobe / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000032:analysis / Q000378:metabolism*; D012727:Sex Characteristics; D013378:Substantia Nigra / Q000378:metabolism; D013787:Thalamic Nuclei / Q000378:metabolism; D000077334:Zolpidem
chemical_list: D011725:Pyridines; D011963:Receptors, GABA-A; D005445:Flunitrazepam; D000077334:Zolpidem