Development of a hypersensitive detection method for human parvovirus B19 DNA.
A new detection method for human parvovirus B19 DNA was established using PCR coupled with a hybridization protection assay. The amplified product was detected using acridinium ester-labeled DNA probes. By this method, a few copies of B19 DNA were detected in human serum albumin.
Sato
K
K
Chugai Pharmaceutical Co. Ltd., Central Research Laboratories, 3-41-8 Takada, Toshima-ku, Tokyo 171-8545, Japan. satokza@chugai-pharm.co.jp
Matsuda
E
E
Kamisango
K
K
Iwasaki
H
H
Matsubara
S
S
Matsunaga
Y
Y
D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D010322:Parvoviridae Infections / Q000097:blood / Q000175:diagnosis*; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D012709:Serum Albumin; D019562:Viral Load
D015342:DNA Probes; D004279:DNA, Viral; D012709:Serum Albumin