A potassium channel protein encoded by chlorella virus PBCV-1.
The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.
Plugge
B
B
Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen, 37073 Göttingen, Germany.
Gazzarrini
S
S
Nelson
M
M
Cerana
R
R
Van Etten
J L
JL
Derst
C
C
DiFrancesco
D
D
Moroni
A
A
Thiel
G
G
D000547:Amantadine / Q000494:pharmacology; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002586:Cesium / Q000494:pharmacology; D002708:Chlorella / Q000821:virology; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009865:Oocytes; D018408:Patch-Clamp Techniques; D017862:Phycodnaviridae / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D015221:Potassium Channels / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D010948:Viral Plaque Assay; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects; D014982:Xenopus laevis
C405463:Kcv potassium channel, Chlorella virus; D015221:Potassium Channels; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014764:Viral Proteins; D002586:Cesium; D001464:Barium; D012964:Sodium; D000547:Amantadine; D011188:Potassium