Apoptosis induction in HCT116 cells by cytochalasins isolated from the fungus Daldinia vernicosa.
We examined the induction of apoptosis by cytochalasin (cc) derivatives (1-14) isolated from the Japanese fungus Daldinia vernicosa to HCT116 human colon cancer cell line based on their cytotoxicity, DNA ladder and DNA fragmentation ratio in agarose gel electrophoresis, and morphological changes. Most cc derivatives tested here induced apoptosis. Particularly cytochalasin 1 (cc1), monoacetate of 1 (cc1Ac), and cc14 were the most potent apoptosis inducers. These apoptotic activities were stronger than that of cytochalasin D as a known apoptosis inducer in HCT116 cell. However, cc4 and cc12 induced necrosis. The structure-activity relationship including their cytotoxicity will be discussed.
Nagasawa
H
H
Department of Biological Science & Technology, Faculty of Engineering, University of Tokushima, Japan.
Nagura
F
F
Mohamad
S B
SB
Uto
Y
Y
Zhu
J W
JW
Tsukuda
T
T
Hashimoto
T
T
Asakawa
Y
Y
Hori
H
H
D017209:Apoptosis / Q000187:drug effects*; D003572:Cytochalasins / Q000302:isolation & purification / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D005658:Fungi / Q000737:chemistry*; D006801:Humans; D014407:Tumor Cells, Cultured
D003572:Cytochalasins