Survey of solasodine-type glycoalkaloids by western blotting and ELISA using anti-solamargine monoclonal antibody.
Solasodine glycosides were analyzed for the selection of higher-producing natural resources in the fruits of Solanum spp. by western blotting and ELISA using anti-solamargine monoclonal antibody (MAb). Western blotting of Solanum khasianum showed a correlation with the analysis by ELISA. The results indicated that the fruits of S. khasianum contained the highest level of solasodine glycosides, compared to other species having 53.43 +/- 3.90 microg/mg dry wt. The high level of solasodine glycosides in S. khasianum suggests that it may be suitable for commercial production.
Putalun
W
W
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Tanaka
H
H
Yahara
S
S
Lhieochaiphan
S
S
Shoyama
Y
Y
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002138:Calibration; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D010840:Phytosterols / Q000276:immunology*; D019657:Solanaceae / Q000737:chemistry*; D012991:Solanaceous Alkaloids / Q000032:analysis* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006027:Glycosides; D010840:Phytosterols; D012991:Solanaceous Alkaloids; C026609:beta-solamarine