title: Distribution of dopamine-immunoreactive fibers in the rat brainstem.
abstract: We describe the distribution of axons immunoreactive for dopamine in pons and medulla oblongata of rat under normal conditions or after inhibition of monoamine oxidase or dopamine beta-hydroxylase. In the pons of non-treated animal, fairly dense plexuses of dopamine-immunoreactive varicose fibers were found in the locus coeruleus, dorsal parabrachial and dorsal raphe nuclei, central gray and reticular formation dorsal to the superior olive. In the medulla oblongata, the immunoreactive fibers were abundant in the dorsal vagal complex, lateral paragigantocellular nucleus, midline raphe nuclei and spinal trigeminal nucleus. Monoamine oxidase inhibition made it possible to increase the intensity of immunoreactivity and consequently the number of labeled fibers in these areas, indicating that dopamine is perpetually oxidized by monoamine oxidase, and consequently in low concentration under normal conditions. Sparse dopamine-immunoreactive fibers were observed in the pontine gray, motor trigeminal nucleus, inferior olive and major axon bundles such as the dorsal and ventral tegmental bundles, where numerous noradrenergic fibers have been reported. In axons of these areas, intense dopamine-immunoreactivity was seen only after inhibition of dopamine-beta-hydroxylase. It appears that dopamine is released and oxidized in response to autonomic changes such as hypoxia, hemorrhage, and cardiovascular variation in the caudal brainstem, as we have described elsewhere.
authors.affiliation: Département de Médecine Expérimentale, INSERM U480, Faculté de Médecine, Université Claude Bernard, 8, avenue Rockefeller, 69373, Lyon, France. kitahama@sommeil.univ-lyon1.fr
authors.forename: K
authors.initials: K
authors.lastname: Kitahama
authors.forename: I
authors.initials: I
authors.lastname: Nagatsu
authors.forename: M
authors.initials: M
authors.lastname: Geffard
authors.forename: T
authors.initials: T
authors.lastname: Maeda
mesh_terms: D000818:Animals; D005406:Bis(4-Methyl-1-Homopiperazinylthiocarbonyl)disulfide / Q000494:pharmacology; D004298:Dopamine / Q000032:analysis / Q000378:metabolism*; D004299:Dopamine beta-Hydroxylase / Q000037:antagonists & inhibitors; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008636:Mesencephalon / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009412:Nerve Fibers / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D010293:Pargyline / Q000494:pharmacology; D011149:Pons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
chemical_list: D008996:Monoamine Oxidase Inhibitors; D005406:Bis(4-Methyl-1-Homopiperazinylthiocarbonyl)disulfide; D010293:Pargyline; D004299:Dopamine beta-Hydroxylase; D004298:Dopamine