Establishment of bovine chromosome region specific high resolution maps using microdissection.
An alternative approach for the direct analysis of chromosome regions corresponding to economical traits on the basis of chromosome microdissection is described. Large fragment clones isolated with primer pairs designed from chromosome fragment-specific DNA sequences were localized by FISH to the scraped chromosome region of interest. The chromosome fragment-specific clones are a useful tool for the generation of region specific high density marker and gene maps and represent the source material for the development of a DNA contig including the economical trait.
Goldammer
T
T
Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Forschungsbereich Molekularbiologie, Dummerstorf, Germany.
Brunner
R M
RM
Kang'a
S
S
Hanotte
O
O
Schwerin
M
M
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000648:ultrastructure*; D003001:Cloning, Molecular; D017931:DNA Primers; D017404:In Situ Hybridization, Fluorescence
D017931:DNA Primers