Isolation, characterization, and chromosomal location of the tRNA(Met) genes in Atlantic salmon (Salmo salar) and brown trout (Salmo trutta).
This work describes the isolation, characterization, and physical location of the methionine tRNA in the genome of Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.). An Atlantic salmon genomic library was screened using a tRNA(Met) probe from Xenopus laevis. Two cosmid clones containing the Atlantic salmon tRNA(Met) gene were isolated, subcloned and sequenced. The tRNA(Met) was mapped to metaphase chromosomes by fluorescence in situ hybridization (FISH). Chromosomal data indicated that the tDNA of methionine is tandemly repeated in a single locus in both species. Analysis of genomic DNA by Southern hybridization confirmed the tandem organization of this gene.
Perez
J
J
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Moran
P
P
Garcia-Vazquez
E
E
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005796:Genes; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012358:RNA, Transfer, Met / Q000235:genetics*; D020317:Salmo salar / Q000235:genetics; D012474:Salmon / Q000235:genetics*; D013045:Species Specificity
D012358:RNA, Transfer, Met