Atypical findings of protein studies using capillary zone electrophoresis.
We present two atypical cases of protein studies that were evaluated by immunofixation and immunosubtraction using capillary zone electrophoresis and high-resolution agarose gel electrophoresis. The first study showed an abnormal peak in the beta region by capillary zone electrophoresis that was located in the gamma region of the high-resolution agarose gel electrophoresis. Further investigation showed that this monoclonal protein was displaced due to binding with beta-lipoproteins. In the second case, a large peak was detected in the alpha-2 region and was shown by capillary zone electrophoresis to be a non-proteinaceous material that mimicked a paraprotein.
Cutlan
R T
RT
Department of Pathology, University of Tennessee, Memphis, USA.
Smalley
D L
DL
Mayer
R P
RP
Mullersman
J E
JE
Blessum
C R
CR
D000368:Aged; D016477:Artifacts; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010265:Paraproteinemias / Q000175:diagnosis; D010266:Paraproteins / Q000032:analysis*
D010266:Paraproteins