HLA-A*2416: a new allele of the HLA-A19 lineage.
We here report on a new HLA-A19 allele (A*2416) found in a German kidney recipient. Serological class I typing revealed HLA-A11,19 without clear definition of the A19 split antigen. As with serology, polymerase chain reaction (PCR)-based typing also revealed inconclusive results. We therefore sequenced the gene from the 5' flanking region through the 3'-end of exon 4 of this allele after haplotype-specific PCR amplification. The sequence analysis revealed a new HLA-A allele which is identical to A*3101 with the exception of the 3' half of exon 2 which is identical to the common A9 alleles. The phylogenetic analysis constructed with the nearest-neighbor algorithm and based on exons 1-4 or introns 1-3 clearly indicated, that A*2416 belongs unequivocally to the A19 lineage.
Binder
T
T
Institute of Transfusion Medicine, Friedrich-Schiller University, Jena, Germany.
Heym
J
J
Horn
B
B
Blasczyk
R
R
D000483:Alleles; D001483:Base Sequence; D018076:DNA, Complementary; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data
D018076:DNA, Complementary; D015234:HLA-A Antigens; C043205:HLA-A19 antigen