Extracellular matrix proteins increase the expression of pro-TRH and pro-protein convertase PC1 in fetal hypothalamic neurons in vitro.
External clues for neuron development include extracellular matrix (ECM) molecules. To explore ECM influence on the early development of peptide phenotype in the CNS, we have compared pro-TRH levels in primary cultures of rat hypothalamic cells plated either on poly-lysine (PL) (control) or on PL plus one of various ECM molecules at 10 microgram/ml. Fetal day 17 cells plated at a density of 1250/mm(2) were grown in a serum free medium made of Neurobasal medium supplemented with B27 (GIBCO). Cultures, consisting mainly of neurons, were analyzed at DIV 2. ECM proteins induced morphological effects in agreement with previously published studies. The amount of pro-TRH per dish, quantified by Western blotting, was increased to 275% for laminin, 191% for fibronectin and 173% for tenascin-C (control=100%); there was no effect of vitronectin. Laminin or fibronectin did not change pro-TRH mRNA or TRH levels but enhanced levels of the pro-protein convertase PC1 suggesting that the ECM molecules did regulate the translational status of pro-TRH. In conclusion, we have shown that some ECM proteins increased pro-TRH level in vitro; this may contribute to the enhancement of pro-TRH levels observed early in vivo in the hypothalamus.
Niquet
J
J
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P. 510-3, Cuernavaca, Mexico.
Pérez-Martínez
L
L
Guerra
M
M
Grouselle
D
D
Joseph-Bravo
P
P
Charli
J
J
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005333:Fetus / Q000166:cytology; D005353:Fibronectins / Q000032:analysis; D018507:Gene Expression Regulation, Developmental; D007031:Hypothalamus / Q000166:cytology* / Q000196:embryology; D066298:In Vitro Techniques; D007797:Laminin / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D043484:Proprotein Convertases; D014176:Protein Biosynthesis / Q000502:physiology; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D019063:Tenascin / Q000032:analysis; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D019096:Vitronectin / Q000032:analysis
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D011498:Protein Precursors; D012333:RNA, Messenger; D019063:Tenascin; D019096:Vitronectin; D013973:Thyrotropin-Releasing Hormone; C046418:pro-thyrotropin releasing hormone; D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases; D011761:Pyrrolidonecarboxylic Acid