title: Crystal structure of the S15-rRNA complex.
abstract: In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. Here we report the 2.8 ¿ resolution crystal structure of the highly conserved S15-rRNA complex. Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. The present structure gives insights into the dual role of S15 in ribosome assembly and translational regulation.
authors.affiliation: Institute of Protein Research, Pushchino, Moscow Region, 142292, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Nikulin
authors.forename: A
authors.initials: A
authors.lastname: Serganov
authors.forename: E
authors.initials: E
authors.lastname: Ennifar
authors.forename: S
authors.initials: S
authors.lastname: Tishchenko
authors.forename: N
authors.initials: N
authors.lastname: Nevskaya
authors.forename: W
authors.initials: W
authors.lastname: Shepard
authors.forename: C
authors.initials: C
authors.lastname: Portier
authors.forename: M
authors.initials: M
authors.lastname: Garber
authors.forename: B
authors.initials: B
authors.lastname: Ehresmann
authors.forename: C
authors.initials: C
authors.lastname: Ehresmann
authors.forename: S
authors.initials: S
authors.lastname: Nikonov
authors.forename: P
authors.initials: P
authors.lastname: Dumas
mesh_terms: D000595:Amino Acid Sequence; D020029:Base Pairing / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites / Q000187:drug effects; D017124:Conserved Sequence / Q000235:genetics; D018360:Crystallography, X-Ray; D008274:Magnesium / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D016963:Thermus thermophilus / Q000737:chemistry* / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; C036004:ribosomal protein S15; D008274:Magnesium