[Influence of transfection of sense- and antisense-TGF beta 1 gene on human pulmonary giant cell carcinoma cell proliferation].
OBJECTIVE
To investigate the effect of human sense- and antisense-transforming growth factor beta 1 (TGF beta 1) gene on human pulmonary giant cell carcinoma cell (PLA-801D) proliferation.


METHODS
PLA-801D cells were transfected with an expression vector containing a full length human sense- or antisense-TGF beta 1 gene by gene transfer. The changes of cell proliferation, expressions of nm23 mRNA and c-myc mRNA and the feature of apoptosis were studied on transfected cells and untransfected cells.


RESULTS
The cells transfected by sense-TGF beta 1 gene showed higher TGF beta 1 activity in the conditioned media, growth inhibition in vitro and decreased coloning formation in anchorage-independent assay. The tumorigenic rate was reduced about 47%, the metastasis was inhibited in athymic nude mice as compared with untransfected cells group. The mechanism of such changes described may be associated with the higher expression of nm23 mRNA, and the lower expression of c-myc mRNA as well as the apoptosis in sense-TGF beta 1 gene transfected cells. However, opposing changes were observed in the antisense-TGF beta 1 gene transfected cells.


CONCLUSIONS
TGF beta 1 acts as a negative growth regulator of the PLA-801D in vitro as well as in vivo.
Yang
H
H
Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing.
Lu
Y
Y
Shen
H
H
D000818:Animals; D016375:Antisense Elements (Genetics) / Q000235:genetics*; D017209:Apoptosis; D018286:Carcinoma, Giant Cell / Q000473:pathology*; D002455:Cell Division; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis; D054778:NM23 Nucleoside Diphosphate Kinases; D009368:Neoplasm Transplantation; D009701:Nucleoside-Diphosphate Kinase; D014157:Transcription Factors / Q000096:biosynthesis; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000502:physiology; D014407:Tumor Cells, Cultured
D016375:Antisense Elements (Genetics); D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins