Cloning and characterization of a gene complementing the mutation of an ethanol-sensitive mutant of sake yeast.
Ethanol-sensitive mutants (esl to es10) were isolated from sake yeast, Saccharomyces cerevisiae SY-32. These mutants were unable to grow at 7% ethanol at which the wild type strain SY-32 does grow. The mutants had a variety of fermentation rates and viabilities in the presence of ethanol. The gene ERG6, complementing the ethanol-sensitive mutation of es5, was cloned from an SY-32 gene library. ERG6 encodes S-adenosylmethionine: delta 24-sterol-C-methyltransferase (EC 2.1.1.41) in the ergosterol synthetic pathway. Mutant es5 had a reduced ability to synthesize ergosterol. An erg6 disruptant was also ethanol-sensitive. These results suggested that ERG6 plays an important role in the ethanol tolerance of S. cerevisiae.
Inoue
T
T
Nishinomiya Syuzo Co., Ltd., Hyogo, Japan. sakarikn@skyblue.ocn.ne.jp
Iefuji
H
H
Fujii
T
T
Soga
H
H
Satoh
K
K
D003001:Cloning, Molecular; D004361:Drug Tolerance / Q000235:genetics; D004875:Ergosterol / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology*; D005816:Genetic Complementation Test; D008780:Methyltransferases / Q000235:genetics; D009154:Mutation; D012275:Oryza; D014920:Wine / Q000382:microbiology*; D015003:Yeasts / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics
D000431:Ethanol; D008780:Methyltransferases; C022670:delta 24-sterol methyltransferase; D004875:Ergosterol