An in vitro system from Plasmodium falciparum active in endogenous mRNA translation.
An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.
Ferreras
A
A
Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad de Carabobo, Maracay, Aragua, Venezuela.
Triana
L
L
Correia
H
H
Sánchez
E
E
Herrera
F
F
D000818:Animals; D002474:Cell-Free System; D004912:Erythrocytes / Q000469:parasitology; D010963:Plasmodium falciparum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D016053:RNA, Protozoan / Q000032:analysis*
D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan