Isolation of monoclonal antibodies directed against the V protein of human parainfluenza virus type 2 and localization of the V protein in virus-infected cells.
Two monoclonal antibodies (mAbs) specific for the human parainfluenza virus type 2 (hPIV-2) V protein were obtained by immunizing mice with the V protein recombinantly expressed in Escherichia coli. Both mAbs were found to react with the V protein in ELISA and in Western blot analysis. Using these mAbs and previously obtained mAbs specific for hPIV-2 nucleoprotein (NP) or hPIV-2 phospho-(P) protein, we examined the intracellular distributions of the V, P and NP proteins in hPIV-2-infected cells by indirect immunofluorescence analyses. The P and NP proteins were organized in numerous granules in the cytoplasm of hPIV-2 infected cells. In contrast, the V protein showed diffuse nuclear and cytoplasm distributions.
Nishio
M
M
Department of Microbiology, Mie University School of Medicine, Japan. nishio@doc.medic.mie-u.ac.jp
Tsurudome
M
M
Ito
M
M
Kawano
M
M
Kusagawa
S
S
Komada
H
H
Ito
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry / Q000821:virology; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000737:chemistry / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006367:HeLa Cells / Q000821:virology; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D009698:Nucleoproteins / Q000276:immunology; D010223:Parainfluenza Virus 2, Human / Q000276:immunology* / Q000302:isolation & purification; D010750:Phosphoproteins / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014709:Vero Cells / Q000821:virology; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C407031:NP protein, Human parainfluenza virus 2; D009698:Nucleoproteins; C407030:P protein, Human parainfluenza virus 2; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; C068854:V protein, human parainfluenza virus type 1; D014764:Viral Proteins; D015678:Viral Structural Proteins