Mammalian cell transformation and aneuploidy induced by five bisphenols.
Bisphenol-A (BP-A), a monomer of plastics used in numerous consumer products and a xenoestrogen, induces cellular transformation and aneuploidy in Syrian hamster embryo (SHE) cells. In this study, the abilities of 4 other bisphenols to induce cellular transformation and genetic effects in SHE cells were examined and compared to BP-A. Cellular growth was inhibited by all bisphenols in a concentration-related manner. The growth inhibitory effect of the bisphenols ranked: BP-5 > BP-4 > BP-3 > BP-2 or BP-A. Morphological transformation of SHE cells was induced by BP-A, BP-3, BP-4 and BP-5, and the induced-transformation frequencies were highest with BP-4. None of the bisphenols induced gene mutations at the Na(+)/K(+) ATPase locus or the hprt locus, or chromosomal aberrations in SHE cells. By contrast, aneuploidy induction in the near-diploid range was exhibited by BP-A, BP-3, BP-4 or BP-5, corresponding to the transforming activity of each compound. The results indicate that BP-A, BP-3, BP-4 and BP-5 exhibit transforming activity in SHE cells, while BP-2 does not, and that aneuploidy induction may be a causal mechanism of the transforming activity.
Tsutsui
T
T
Department of Pharmacology, The Nippon Dental University, Tokyo, Japan.
Tamura
Y
Y
Suzuki
A
A
Hirose
Y
Y
Kobayashi
M
M
Nishimura
H
H
Metzler
M
M
Barrett
J C
JC
D000782:Aneuploidy; D000818:Animals; D001559:Benzhydryl Compounds; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D006224:Cricetinae; D004622:Embryo, Mammalian; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008647:Mesocricetus; D016296:Mutagenesis; D010636:Phenols / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D007041:Hypoxanthine Phosphoribosyltransferase; D010636:Phenols; D000254:Sodium-Potassium-Exchanging ATPase; C006780:bisphenol A; D000098826:Bisphenol A Compounds