title: The targeting and accumulation of ectopically expressed oleosin in non-seed tissues of Arabidopsis thaliana.
abstract: Full-length and N-terminal deletions of a sunflower (Helianthus annuus L.) oleosin protein were expressed ectopically in transgenic Arabidopsis thaliana (L.) Heynh. Immunological detection of the sunflower protein revealed that it accumulated in a range of non-oil-storing tissues, including leaves, roots and petals. This accumulation was shown to result from deposition in the microsomal membrane fraction. Expression in oil-storing tissues (such as seeds) of oleosin N-terminal deletions revealed impaired transfer from the endoplasmic reticulum to the oil body. In non-oil-storing tissues, accumulation in the microsomal membrane fraction was progressively reduced by N-terminal deletion. These data confirm the role of the endomembrane system in the targeting of the oleosin and its intimate relationship with oil-body biogenesis.
authors.affiliation: IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Briston, Long Ashton, UK.
authors.forename: F
authors.initials: F
authors.lastname: Beaudoin
authors.forename: J A
authors.initials: JA
authors.lastname: Napier
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D006368:Helianthus / Q000235:genetics*; D008861:Microsomes / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D018514:Plant Structures / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D010938:Plant Oils; D010940:Plant Proteins; D011994:Recombinant Proteins; C079350:oleosin, Helianthus annuus