title: Transient and permanent resolution of ischemic lesions on diffusion-weighted imaging after brief periods of focal ischemia in rats : correlation with histopathology.
abstract: OBJECTIVE
  The early ischemic lesions demonstrated by diffusion-weighted imaging (DWI) are potentially reversible. The purposes of this study were to determine whether resolution of initial DWI lesions is transient or permanent after different brief periods of focal brain ischemia and to evaluate histological outcomes.
  
  
  METHODS
  Sixteen rats were subjected to 10 minutes (n=7) or 30 minutes (n=7) of temporary middle cerebral artery occlusion or sham operation (n=2). DWI, perfusion-weighted imaging (PWI), and T(2)-weighted imaging (T(2)WI) were performed during occlusion; immediately after reperfusion; and at 0.5, 1.0, 1.5, 12, 24, 48, and 72 hours after reperfusion. After the last MRI study, the brains were fixed, sectioned, stained with hematoxylin and eosin, and evaluated for neuronal necrosis.
  
  
  RESULTS
  No MRI or histological abnormalities were observed in the sham-operated rats. In both the 10-minute and 30-minute groups, the perfusion deficits and DWI hyperintensities that occurred during occlusion disappeared shortly after reperfusion. The DWI, PWI, and T(2)WI results remained normal thereafter in the 10-minute group, whereas secondary DWI hyperintensity and T(2)WI abnormalities developed at the 12-hour observation point in the 30-minute group. Histological examinations demonstrated neuronal necrosis in both groups, but the number of necrotic neurons was significantly higher in the 30-minute group (95+/-4%) than in the 10-minute group (17+/-10%, P<0.0001).
  
  
  CONCLUSIONS
  Transient or permanent resolution of initial DWI lesions depends on the duration of ischemia. Transient resolution of DWI lesions is associated with widespread neuronal necrosis; moreover, permanent resolution of DWI lesions does not necessarily indicate complete salvage of brain tissue from ischemic injury.
authors.affiliation: Departments of Neurology, UMass Memorial Health Care, Worcester, MA 01605, USA. fhli@wpi.edu
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: K F
authors.initials: KF
authors.lastname: Liu
authors.forename: M D
authors.initials: MD
authors.lastname: Silva
authors.forename: T
authors.initials: T
authors.lastname: Omae
authors.forename: C H
authors.initials: CH
authors.lastname: Sotak
authors.forename: J D
authors.initials: JD
authors.lastname: Fenstermacher
authors.forename: M
authors.initials: M
authors.lastname: Fisher
authors.forename: C Y
authors.initials: CY
authors.lastname: Hsu
authors.forename: W
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000175:diagnosis* / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008297:Male; D009336:Necrosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D013997:Time Factors