title: Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin 3.
abstract: A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients.
authors.affiliation: Service de Pharmacie-Toxico-Pharmacologie, Hôpital Robert Debre, Paris, France. francoise.brion@rdb.ap-hop-paris.fr
authors.forename: A
authors.initials: A
authors.lastname: Paci
authors.forename: A
authors.initials: A
authors.lastname: Rieutord
authors.forename: D
authors.initials: D
authors.lastname: Guillaume
authors.forename: F
authors.initials: F
authors.lastname: Traoré
authors.forename: J
authors.initials: J
authors.lastname: Ropenga
authors.forename: H P
authors.initials: HP
authors.lastname: Husson
authors.forename: F
authors.initials: F
authors.lastname: Brion
mesh_terms: D000171:Acrolein / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011807:Quinolizines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; C409683:Luminarin 3; D011807:Quinolizines; D000171:Acrolein