Angiotensin II modulates the activity of the Na+/K+ATPase in eel kidney.
We have previously shown that angiotensin II (Ang II) has a role at the level of the eel gill chloride cell regulating sodium balance, and therefore osmoregulation; the purpose of the present study was to extend these findings to another important osmoregulatory organ, the kidney. By catalytic histochemistry Na(+)/K(+)ATPase activity was found in both sea water (SW)- and freshwater (FW)-adapted eel kidney, particularly at the level of both proximal and distal tubules. Quantitation of tubular cell Na(+)/K(+)ATPase activity, by imaging, gave values in SW-adapted eels which were double those found in FW-adapted eels (Student's t-test: P<0.0001). This was due to a reduced number of positive tubules present in FW-adapted eels compared with SW-adapted eels. By conventional enzymatic assay, the Na(+)/K(+)ATPase activity in isolated tubular cells from SW-adapted eels showed values 1.85-fold higher those found in FW-adapted eels (Student's ttest: P<0.0001). Perfusion of kidney for 20 min with 100 nM Ang II provoked a significant increase (1.8-fold) in Na(+)/K(+)ATPase activity in FW, due to up-regulation of Na(+)/K(+)ATPase activity in a significantly larger number of tubules (Student's t-test: P<0.0001). The effect of 100 nM Ang II in SW-adapted kidneys was not significant. Stimulation with increasing Ang II concentrations was performed on isolated kidney tubule cells: Ang II provoked a dose-dependent stimulation of the Na(+)/K(+)ATPase activity in FW-adapted eels, reaching a maximum at 100 nM (1.82-fold stimulation), but no significant effect was found in SW-adapted eels (ANOVA: P<0.001 and P>0.05 respectively). Isolated tubule cells stimulated with 100 nM Ang II showed a significant generation of inositol trisphosphate (InsP(3)) and an increment in calcium release from intracellular stores. In conclusion, our results suggest that tubular Na(+)/K(+)ATPase is modulated by environmental salinity, and that Ang II has a role in regulating its activity in FW-adapted eels, probably through an InsP(3)-dependent mechanism.
Marsigliante
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S
Dipartimento di Biologia, Laboratorio di Fisiologia, Università di Lecce, Via Provinciale per Monteroni, 73100, Lecce, Italy. smarsi@ilenic.unile.it
Muscella
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A
Barker
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S
Storelli
C
C
D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004524:Eels / Q000502:physiology*; D005618:Fresh Water; D007668:Kidney / Q000201:enzymology*; D012623:Seawater; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*; D015854:Up-Regulation; D014882:Water-Electrolyte Balance / Q000502:physiology
D000804:Angiotensin II; D000254:Sodium-Potassium-Exchanging ATPase