Comparison of the hypersensitive response induced by the tomato Cf-4 and Cf-9 genes in Nicotiana spp.
We have previously shown that tomato Cf-9 induces an Avr9-dependent hypersensitive response (HR) in Nicotiana tabacum and potato. We show here that Cf-4 also induces an Avr4-dependent HR in two tobacco species (N. tabacum and N. benthamiana). The HR induced by Cf-4 and Cf-9 was compared in stable tobacco transgenics by a seedling lethal assay and resistance to recombinant Potato virus X expressing Avr4 or Avr9. We also compared HR induction with Agrobacterium-mediated transient expression. The Cf-4/Avr4 combination induced a more rapid HR than Cf-9/Avr9. Sensitive assays for Cf-9 and Cf-4 function should prove useful for structure/function analyses of these resistance proteins in tobacco.
Thomas
C M
CM
Sainsbury Laboratory, John Innes Centre, Norwich Research Park, UK.
Tang
S
S
Hammond-Kosack
K
K
Jones
J D
JD
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D005656:Fungal Proteins / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000821:virology; D010947:Plants, Toxic; D017863:Potexvirus / Q000472:pathogenicity*; D014026:Nicotiana / Q000235:genetics* / Q000821:virology
C085987:AVR4 protein, Cladosporium fulvum; C109609:Cf protein, Lycopersicon esculentum; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D010940:Plant Proteins; C073605:AVR9 protein, Cladosporium fulvum