Investigation of spectroscopic intermediates during copper-binding and TPQ formation in wild-type and active-site mutants of a copper-containing amine oxidase from yeast.
Copper amine oxidases possess the unusual ability to generate autocatalytically their organic cofactor, which is subsequently utilized in turnover. This cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ), is formed within the active site of these enzymes by the oxidation of a single tyrosine residue. In vitro, copper(II) and oxygen are both necessary and sufficient for the conversion of tyrosine to TPQ. In this study, the biogenesis of TPQ has been characterized in an amine oxidase from Hansenula polymorpha expressed as the apo-enzyme in Escherichia coli. With the WT enzyme, optical absorbances which are copper or oxygen dependent are observed and characterized. Active-site mutants are used to investigate further the nature of these spectral species. Evidence is presented which suggests that tyrosine is activated for reaction with oxygen by liganding to Cu(II). In the following paper in this issue [Schwartz, B., Dove, J. E., and Klinman, J. P. (2000) Biochemistry 39, 3699-3707], the initial reaction of precursor protein with oxygen is characterized kinetically. Taken together, the available data suggest a mechanism for the oxidation of tyrosine to TPQ where the role of the copper is to activate substrate.
Dove
J E
JE
Departments of Chemistry and Molecular and Cell Biology, University of California, Berkeley, 94720, USA.
Schwartz
B
B
Williams
N K
NK
Klinman
J P
JP
D006631:Amine Oxidase (Copper-Containing) / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001216:Asparagine / Q000235:genetics; D001224:Aspartic Acid / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D003545:Cysteine / Q000235:genetics; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D018698:Glutamic Acid / Q000235:genetics; D005973:Glutamine / Q000235:genetics; D006639:Histidine / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010101:Oxygen Consumption / Q000235:genetics; D010843:Pichia / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013054:Spectrophotometry, Atomic; D013056:Spectrophotometry, Ultraviolet
C086758:2,4,5-trihydroxyphenylalanine quinone; D003067:Coenzymes; D011994:Recombinant Proteins; D005973:Glutamine; D001224:Aspartic Acid; D018698:Glutamic Acid; D006639:Histidine; D004295:Dihydroxyphenylalanine; D001216:Asparagine; D003300:Copper; D006631:Amine Oxidase (Copper-Containing); D003545:Cysteine