Mapping of four simple sequence repeat (SSR) markers on rat chromosome 4.
We previously reported that several markers on rat chromosome (Chr) 4 cosegregated with the occurrence of cerebral stroke and brain edema in stroke-prone spontaneously hypertensive rats (SHRSP). To obtain insights into the positional candidate genes for stroke susceptibility in this region, we mapped four genes, Taurine transporter (Tau), tumor necrosis factor receptor (Tnfr), GABA transporter (Gat1) and glucose transporter-3 (Glut3) genes, using newly developed simple sequence repeat (SSR) markers on rat Chr 4. We isolated the SSRs for the genes either by screening a rat genomic library or by searching the GenBank database. By linkage analysis using two sets of backcrosses, Gat1 and Tnfr were mapped in the region associated with stroke, while Taut was located distant from the region. The Glut3 locus was also assigned to rat Chr 4 using a rat x mouse hybrid clone panel. These results indicated that the Tnfr, Gat1 and Glut3 genes were good positional candidates for the stroke susceptibility in SHRSP, suggesting that further evaluation of these genes by functional studies could prove useful.
Mashimo
T
T
Graduate School of Human and Environmental Studies, Kyoto University, Japan.
Nabika
T
T
Kitada
K
K
Serikawa
T
T
Matsumoto
C
C
Ikeda
K
K
Nara
Y
Y
Masuda
J
J
Yamori
Y
Y
Ohno
Y
Y
Saruta
T
T
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D050485:GABA Plasma Membrane Transport Proteins; D008040:Genetic Linkage; D005819:Genetic Markers; D015698:Genomic Library; D005838:Genotype; D051274:Glucose Transporter Type 3; D008562:Membrane Glycoproteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D018598:Minisatellite Repeats / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins; D027361:Organic Anion Transporters; D051381:Rats; D011914:Rats, Inbred BN / Q000235:genetics; D011918:Rats, Inbred SHR / Q000235:genetics; D011921:Rats, Inbred WKY / Q000235:genetics; D020318:Rats, Long-Evans / Q000235:genetics; D017208:Rats, Wistar / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013654:Taurine / Q000235:genetics; D005680:gamma-Aminobutyric Acid / Q000378:metabolism; D000099247:Taurine Transporters
D002352:Carrier Proteins; D004247:DNA; D017931:DNA Primers; D050485:GABA Plasma Membrane Transport Proteins; D005819:Genetic Markers; D051274:Glucose Transporter Type 3; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D027361:Organic Anion Transporters; D018124:Receptors, Tumor Necrosis Factor; D013654:Taurine; D005680:gamma-Aminobutyric Acid; D000099247:Taurine Transporters; C495690:Slc2a3 protein, rat; C493414:Slc6a1 protein, mouse; C493415:Slc6a1 protein, rat