Comparison of skin prick test with serially diluted wild-type and genetically engineered recombinant Der f2.
BACKGROUND
C8/119S, a genetically engineered less allergenic mutant of group II allergen (Der f2) of house dust mite, Dermatophagoidesfarinae, was constructed in order to reduce the risk of anaphylactic reactions of allergen specific immunotherapy.


OBJECTIVE
To further evaluate, with a larger number of mite-allergic patients, the safety of C8/119S for the treatments of humans.


METHODS
We tested the dose-dependent responses of 20 mite-allergic volunteers to a skin prick test with C8/119S and wild-type recombinant Der f2 and compared the biologic potentials of these allergens to induce type I allergic reactions. In a separate experiment, we compared IgE binding capacities to C8/119S and to wild-type recombinant Der f2 in individual sera from 34 mite-allergic donors.


RESULTS
The concentration of C8/119S needed to induce positive skin prick test (SPT) reaction was at least 100 times more than that of recombinant Der f2 in 95% of the volunteers tested. Consistent with this result, IgE binding data showed that 85% of the mite-allergic donors had little or no detectable IgE bound to C8/119S. Our data also shows that a minority of mite-allergic patients responded in a similar manner to both C8/119S and wild-type recombinant Der f2.


CONCLUSIONS
Our data confirms that C8/119S is much less allergenic and thus can be used safely for immunotherapy of most of mite-allergic patients. Care should still be taken because, in a minority of patients, C8/119S may cause similar type I allergic reactions as does wild-type recombinant Der f2.
Kusunoki
T
T
Department of Pediatrics, Kyoto University, Japan. kusu@kuhp.kyoto-u.ac.jp
Inoue
Y
Y
Korematsu
S
S
Harazaki
M
M
Yokota
T
T
Hosoi
S
S
D000328:Adult; D000818:Animals; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism; D008925:Mites / Q000276:immunology; D009154:Mutation; D011485:Protein Binding / Q000276:immunology; D011852:Radioallergosorbent Test; D011994:Recombinant Proteins / Q000276:immunology; D012882:Skin Tests
D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E