title: Sensitivity enhancement of HCACO by using an HMQC magnetization transfer scheme.
abstract: Previous theoretical calculations have demonstrated that the multiquantum relaxation rate of (1)H(alpha)-(13)C(alpha)(R(MQ)) is, on average, 1.3 +/- 0.4 or 1.7 +/- 0.6 times slower than the single-quantum relaxation rate of (13)C(alpha)(R(C)) for a sample with or without, respectively, amide protons. By taking advantage of this fact and by using the PEP sensitivity enhancement scheme, an HMQC version of the HCACO experiment has been developed. We demonstrate that this new experiment is 23 and 55% more sensitive than the original HSQC version of the HCACO experiment, at constant times of 7 and 27 ms, respectively, for a sample of the BC domain of the ciliary neurotrophic factor receptor protein dissolved in D(2)O at 20 degrees C.
authors.affiliation: Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
authors.forename: Y
authors.initials: Y
authors.lastname: Xia
authors.forename: X
authors.initials: X
authors.lastname: Kong
authors.forename: D K
authors.initials: DK
authors.lastname: Smith
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Man
authors.forename: G
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000465:Algorithms; D000577:Amides / Q000737:chemistry; D002247:Carbon Isotopes; D017666:Deuterium Oxide; D004578:Electron Spin Resonance Spectroscopy; D004735:Energy Transfer; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008280:Magnetics; D008956:Models, Chemical; D011522:Protons; D011789:Quantum Theory; D020801:Receptor, Ciliary Neurotrophic Factor / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000577:Amides; D002247:Carbon Isotopes; D011522:Protons; D020801:Receptor, Ciliary Neurotrophic Factor; D006859:Hydrogen; D017666:Deuterium Oxide