Expression of the basal cell adhesion molecule (B-CAM) in normal and diseased human skin.
The basal cell adhesion molecule (B-CAM) is a 90-kD cell surface glycoprotein with a characteristic immunoglobulin domain structure. The pattern of B-CAM expression in cultured cells suggests that the molecule is associated with a substrate-adherent growth pattern in some lineages. We investigated the expression of B-CAM in normal and diseased human epidermis by means of immunohistochemistry employing a single batch of high-titer mouse monoclonal antibody G253. Snap-frozen biopsy material from normal skin (n = 8), psoriasis (n = 5), contact dermatitis (n = 6), basal cell carcinoma (n = 5) and fetal skin (n = 6) was studied. In normal human skin, B-CAM was found in varying degrees throughout the epidermis with a preference for suprabasal expression, hair follicles were regularly of a B-CAM-positive phenotype. There were no qualitative differences with regard to the B-CAM expression pattern in normal skin in comparison to psoriasis and contact dermatitis. In contrast, fetal skin (15th to 18th week of gestation) was characterized by B-CAM-positive cells in the basal layer of the epidermis as well as in the outer root sheath of hair follicles. Basal cell carcinomas also regularly expressed high levels of B-CAM. A strong B-CAM-positive phenotype can be found in the outer root sheath of hair follicles of adult and fetal human skin as well as in fetal basal keratinocytes.
Bernemann
T M
TM
Department of Dermatology, University of Frankfurt, Germany.
Podda
M
M
Wolter
M
M
Boehncke
W H
WH
D002280:Basal Cell Carcinoma / Q000378:metabolism / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003877:Dermatitis, Contact / Q000378:metabolism / Q000473:pathology; D005314:Embryonic and Fetal Development; D005333:Fetus; D018859:Hair Follicle / Q000196:embryology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism; D008188:Lutheran Blood-Group System; D009363:Neoplasm Proteins / Q000378:metabolism*; D011565:Psoriasis / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000196:embryology / Q000378:metabolism* / Q000473:pathology; D012871:Skin Diseases / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology
C090228:BCAM protein, human; D015815:Cell Adhesion Molecules; D008188:Lutheran Blood-Group System; D009363:Neoplasm Proteins