title: Transgenic expression of granulocyte-macrophage colony-stimulating factor induces the differentiation and activation of a novel dendritic cell population in the lung.
abstract: The role of granulocyte-macrophage colony-stimulating factor (GM-CSF) in the differentiation of dendritic cells (DCs) during pulmonary viral infection was investigated by using a mouse model of GM-CSF transgene expression established with an adenoviral vector (AdGM-CSF). GM-CSF gene transfer resulted in increased levels of GM-CSF in the lung, which peaked at day 4 and remained increased up to day 19. A striking cellular response composed predominantly of macrophage-like cells was observed in the lung receiving AdGM-CSF but not control vector. By FACS analysis, the majority of these cells were identified at an early time point as macrophages and later as mature/activated myeloid DCs characterized by CD11b(bright), CD11c(bright), MHC class II(bright), and B7.1(bright). In contrast, GM-CSF had a weak effect on a small DC population that was found present in normal lung and was characterized by CD11c(bright) and CD11b(low). By immunohistochemistry staining for MHC II, the majority of activated antigen-presenting cells were localized to the airway epithelium and peribronchial/perivascular areas in the lung. A concurrently enhanced Th1 immune response was observed under these conditions. The number of CD4 and CD8 T cells was markedly increased in the lung expressing GM-CSF, accompanied by increased release of interferon (IFN)gamma in the lung. Furthermore, lymphocytes isolated from either lung parenchyma or local draining lymph nodes of these mice but not the control mice released large amounts of IFNgamma on adenoviral antigen stimulation in vitro. These findings reveal that GM-CSF promotes the differentiation and activation of a myeloid DC population primarily by acting on macrophages during pulmonary immune responses.
authors.affiliation: Department of Pathology, Division of Infectious Diseases, Centre for Gene Therapeutics, McMaster University, Hamilton, ON, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: D P
authors.initials: DP
authors.lastname: Snider
authors.forename: B R
authors.initials: BR
authors.lastname: Hewlett
authors.forename: N W
authors.initials: NW
authors.lastname: Lukacs
authors.forename: J
authors.initials: J
authors.lastname: Gauldie
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.forename: Z
authors.initials: Z
authors.lastname: Xing
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology; D018845:CD11 Antigens / Q000032:analysis; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007700:Kinetics; D008168:Lung / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D018655:Lymphocyte Count; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D014162:Transfection
chemical_list: D018845:CD11 Antigens; D000949:Histocompatibility Antigens Class II; D016178:Granulocyte-Macrophage Colony-Stimulating Factor