title: Cytokine expression in vitro by cultured human endothelial cells in contact with polyethylene terephthalate coated with pyrolytic carbon and collagen.
abstract: In order to evaluate whether or not polyethylene terephthalate coated with pyrolytic carbon and collagen (PET+PC) favors inflammatory or hyperplastic reactions, the expression of mRNAs specific for interleukin-6 (IL-6), platelet-derived growth factor-A (PDGF-A), PDGF-B, transforming growth factor-beta1 (TGF-beta1), and TGF-beta2 were tested in vitro by cultured human umbilical vein endothelial cells (HUVEC). The cultures were put in contact with PET+PC for 1, 24, 48, and 72 h. The same cells cultured on polystyrene without biomaterials were tested as negative controls; cultures incubated with LPS were the positive control. The expression of mRNAs was evaluated by RT-PCR with specific primers. PET+PC did not determine any differences in the expression of IL-6-specific mRNA at any of the incubation times compared to the negative control while LPS (the positive control) induced expression after 24, 48, and 72 h. PET+PC induced a more precocious expression of mRNA specific for PDGF-A than did the negative control; however, the expression no longer was present after 48 h while in the negative control the expression stopped after 72 h. PET+PC induced a less frequent expression of PDGF-B-specific mRNA than did the negative control and LPS, especially after 24 h. PET+PC induced a later expression of TGF-beta2-specific mRNA than did the negative control and a less frequent expression of mRNA specific for TGF-beta1 after 24 and 72 h.
authors.affiliation: Istituti Ortopedici Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Cenni
authors.forename: D
authors.initials: D
authors.lastname: Granchi
authors.forename: G
authors.initials: G
authors.lastname: Ciapetti
authors.forename: L
authors.initials: L
authors.lastname: Savarino
authors.forename: A
authors.initials: A
authors.lastname: Corradini
authors.forename: A
authors.initials: A
authors.lastname: Di Leo
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002244:Carbon / Q000494:pharmacology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics*; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics; D010982:Platelet-Derived Growth Factor / Q000235:genetics; D011093:Polyethylene Terephthalates / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics; D014471:Umbilical Veins
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D015850:Interleukin-6; D010982:Platelet-Derived Growth Factor; D011093:Polyethylene Terephthalates; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C075278:platelet-derived growth factor A; C009139:pyrolytic carbon; D002244:Carbon; D003094:Collagen