Determination of nicarbazin in feeds using liquid chromatography-electrospray mass spectrometry.
A method is presented for the determination of the 4,4'-dinitrocarbanilide component of the coccidiostat nicarbazin in animal feeds. Samples are extracted by shaking with methanol and analysed, without further clean-up, using liquid chromatography-electrospray mass spectrometry. A deuterated form of the analyte is employed as internal standard to improve the repeatability of the method. The method has been validated at levels between 0.1 and 100 mg kg-1 with internal standard corrected recoveries between 88 and 101% and RSD values < 8%.
Cannavan
A
A
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast, UK.
Ball
G
G
Kennedy
D G
DG
D000821:Animal Feed / Q000032:analysis*; D002853:Chromatography, Liquid; D003049:Coccidiostats / Q000032:analysis*; D013058:Mass Spectrometry; D009528:Nicarbazin / Q000032:analysis*
D003049:Coccidiostats; D009528:Nicarbazin