title: Collecting duct (Na+/K+)-ATPase activity is correlated with urinary sodium excretion in rat nephrotic syndromes.
abstract: In puromycin aminonucleoside (PAN)-treated nephrotic rats, sodium retention is associated with increased (Na+/K+)-ATPase activity in the cortical collecting ducts (CCD). This study was undertaken to determine whether stimulation of (Na+/K+)-ATPase in the CCD is a feature of other experimental nephrotic syndromes, whether it might be responsible for renal sodium retention, and whether it is mediated by increased plasma vasopressin levels or activation of calcineurin. For this purpose, the time courses of urinary excretion of sodium and protein, sodium balance, ascites, and (Na+/K+)-ATPase activities in microdissected CCD were studied in rats with PAN or adriamycin nephrosis or HgCl2 nephropathy. The roles of vasopressin and calcineurin in PAN nephrosis were evaluated by measuring these parameters in Brattleboro rats and in rats treated with cyclosporin or tacrolimus. Despite different patterns of changes in urinary sodium and protein excretion in the three nephrotic syndrome models, there was a linear relationship between CCD (Na+/K+)-ATPase activities and sodium excretion in all three cases. The results also indicated that there was no correlation between proteinuria and sodium retention, but ascites was present only when proteinuria was associated with marked reduction of sodium excretion. Finally, the lack of vasopressin in Brattleboro rats or the inhibition of calcineurin by administration of either cyclosporin or tacrolimus did not prevent development of the nephrotic syndrome in PAN-treated rats or stimulation of CCD (Na+/K+)-ATPase. It is concluded that stimulation of Na(+/K+)-ATPase in the CCD of nephrotic rats might be responsible for sodium retention and that this phenomenon is independent of proteinuria and vasopressin and calcineurin activities.
authors.affiliation: Service de Néphrologie Pédiatrique, Hôpital Armand-Trousseau, Assistance Publique-Hôpitaux de Paris, Paris, France.
authors.forename: Georges
authors.initials: G
authors.lastname: Deschênes
authors.affiliation: Laboratoire de Biologie Intégrée des Cellules Rénales, Centre National de la Recherche Scientifique Unité de Recherche Associée 1859, Service de Biologie Cellulaire, Commissariat à l'Energie Atomique, Saclay, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Doucet
mesh_terms: D000818:Animals; D019703:Calcineurin / Q000502:physiology; D004317:Doxorubicin; D015433:Glomerulonephritis, Membranous / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology; D007685:Kidney Tubules, Collecting / Q000201:enzymology*; D008297:Male; D008627:Mercuric Chloride; D009318:Natriuresis; D009401:Nephrosis / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology; D009404:Nephrotic Syndrome / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology*; D011692:Puromycin Aminonucleoside; D051381:Rats; D011910:Rats, Brattleboro / Q000502:physiology; D011914:Rats, Inbred BN; D020318:Rats, Long-Evans / Q000502:physiology; D017207:Rats, Sprague-Dawley / Q000502:physiology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D008627:Mercuric Chloride; D011692:Puromycin Aminonucleoside; D004317:Doxorubicin; D019703:Calcineurin; D000254:Sodium-Potassium-Exchanging ATPase