title: [Microbiological study of gallbladder bile in a high risk zone for gallbladder cancer].
abstract: BACKGROUND
  Gallbladder cancer is frequent in Chile and there is sparse information about the association between this type of cancer and the presence of bacteria in the gallbladder bile.
  
  
  OBJECTIVE
  To determine the presence of aerobic bacteria in gallbladder bile in patients with and without gallbladder cancer.
  
  
  METHODS
  A microbiological analysis of bile and pathological study was performed in 608 gallbladders, obtained during to cholecystectomies performed to 513 women and 95 men aged 44 years old as a mean.
  
  
  RESULTS
  Pathological study showed a chronic cholecystitis in 468 cases (77%), an acute cholecystitis in 140 (33%), cancer in 24 (3.9%) and dysplasia in 5 cases (0.8%). A positive culture was obtained in 22.5% of women and 28.5% of males. Twenty seven percent of women over 30 years old had positive cultures compared with 10% of younger women (p < 0.001). Thirty two percent of acute cholecystitis had positive cultures, compared with 24% of chronic cholecystitis (p = 0.03). E Coli was isolated in 51% of positive cases, Streptococci-Enterococci in 24%, Enterobacter sp in 9%, Klebsiella and Proteus in lower proportions. Salmonella sp was isolated in 4 cases, being all women with chronic cholecystitis. Thirteen of 29 cases with cancer or dysplasia had positive cultures (45%), compared with 25% of patients with inflammatory gallbladder diseases (p = 0.02). Streptococci-Enterococci were isolated in 7 cases and Enterobacter sp in three.
  
  
  CONCLUSIONS
  The presence of Salmonella sp in gallbladder bile was not frequent in the studied patients. Its role in the pathogenesis of gallbladder cancer must be reassessed.
authors.forename: I
authors.initials: I
authors.lastname: Roa
authors.forename: G
authors.initials: G
authors.lastname: Ibacache
authors.forename: J
authors.initials: J
authors.lastname: Carvallo
authors.forename: A
authors.initials: A
authors.lastname: Melo
authors.forename: J
authors.initials: J
authors.lastname: Araya
authors.forename: X
authors.initials: X
authors.lastname: De Aretxabala
authors.forename: M
authors.initials: M
authors.lastname: Figueroa
authors.forename: F
authors.initials: F
authors.lastname: Barrientos
authors.forename: C
authors.initials: C
authors.lastname: Figueroa
mesh_terms: D000328:Adult; D000368:Aged; D001420:Bacteria, Aerobic / Q000302:isolation & purification*; D001646:Bile / Q000382:microbiology*; D005260:Female; D005704:Gallbladder / Q000382:microbiology*; D005705:Gallbladder Diseases / Q000175:diagnosis / Q000382:microbiology; D005706:Gallbladder Neoplasms / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012307:Risk Factors