title: Purification and characterization of a human factor that assembles and remodels chromatin.
abstract: We have previously reported the isolation and characterization of a nucleosome remodeling and spacing factor, RSF. One of the RSF subunits is hSNF2h, a SNF2 homologue. Here we set out to isolate and characterize other hSNF2h-containing complexes. We have identified a novel hSNF2h complex that facilitates ATP-dependent chromatin assembly with the histone chaperone NAP-1. The complex possesses ATPase activity that is DNA-dependent and nucleosome-stimulated. This complex is capable of facilitating ATP-dependent nucleosome remodeling and transcription initiation from chromatin templates. In addition to hSNF2h, this complex also contains a 190-kDa protein encoded by the BAZ1A gene. Since both subunits are homologues of the Drosophila ACF complex (ATP-utilizing chromatin assembly and remodeling factor), we have named this factor human ACF or hACF.
authors.affiliation: Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
authors.forename: G
authors.initials: G
authors.lastname: LeRoy
authors.forename: A
authors.initials: A
authors.lastname: Loyola
authors.forename: W S
authors.initials: WS
authors.lastname: Lane
authors.forename: D
authors.initials: D
authors.lastname: Reinberg
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004330:Drosophila; D006367:HeLa Cells; D006801:Humans; D009707:Nucleosomes / Q000502:physiology* / Q000648:ultrastructure; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009707:Nucleosomes; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C409462:SMARCA5 protein, human