Mild hyperthermia-induced apoptosis is dependent on p53 in human lymphoid cells.
Mild hyperthermia is known to enhance apoptosis. The p53 tumor-suppressor gene product has been shown to function in apoptosis in response to genotoxic stress. However, there is little information regarding the mechanism of p53-dependent apoptosis induced by heat stress. In present study, a p53 contribution in mild hyperthermia-induced apoptosis was investigated in human lymphoid system. After 30-minute exposure at 44 degrees C, the accumulation of p53 protein was clearly observed in TK6 and ML-1 cells. Using comet assay, the more significant sensitivity to hyperthermic apoptosis was found in TK6 (wild-type p53) than in WI-L2-NS (mutated in p53). Furthermore, the significantly rapid shifting from early apoptotic phase to late apoptotic was observed in heat-induced p53 TK6 cells. These findings suggest that p53-dependent apoptosis is efficaciously induced by mild hyperthermia as non-genotoxic stress in human lymphoid system.
Seo
Y R
YR
Toxicology Laboratory, Korea Institute of Science and Technology, Cheongryang, Seoul.
Smith
M L
ML
Han
S S
SS
Fairbairn
D W
DW
O'Neill
K L
KL
Ryu
J C
JC
D017209:Apoptosis / Q000502:physiology*; D002460:Cell Line; D020552:Comet Assay; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D009154:Mutation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53