[TGF beta 1 on murine tumor growth following direct intratumoral injection of plasmid DNA].
OBJECTIVE
To investigate TGF beta 1 gene expression and its effect on murine tumor growth following direct intratumoral injection of naked plasmid DNA encoding human TGF beta 1.


METHODS
LM3 murine lung adenocarcinoma cells, were injected subcutaneously to T739 mice and grew to tumor nodules in 2 weeks, Multiple direct intratumoral injections of plasmid DNA, PMAM-neo-TGF beta 1, were given and were compared with saline or vector plasmid administration groups. The growth of tumor was observed till the 8th week when the mice were killed for Northern blot analysis and histopathological study of tumoral tissue.


RESULTS
The growth of tumor was boosted in the TGF beta 1 gene treated mice as compared with the control groups, whereas there was no significant difference in the metastatic behavior. Northern blot showed efficient expression of TGF beta 1 mRNA in the treated group.


CONCLUSIONS
TGF beta 1 may stimulate tumor growth in vivo through certain mechanisms. Direct intratumoral injection of nude plasmid DNA may be a promising gene transfer strategy in vivo.
Gao
P
P
General Hospital PLA, Beijing.
Lu
Y
Y
Ge
X
X
D000230:Adenocarcinoma / Q000473:pathology / Q000628:therapy*; D000818:Animals; D004247:DNA / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000628:therapy*; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D016212:Transforming Growth Factor beta; D004247:DNA