title: E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow.
abstract: The aim of this study was to determine whether colon cancer cells flowing in blood exhibit the same adhesion pattern to the vascular bed as leucocytes using a flow adhesion system. In shear flow conditions, five colon cancer cell lines showed less tethering to E-selectin substrates than polymorphonuclear cells (PMN). However, some of the Colo201 cells formed complete arrest on E-selectin in continuous shear flow which was never observed in PMN cells. Colo201 cells expressed both sialyl Le-x and sialyl Le-a at similar levels in flow cytometry. However, the staining pattern showed marked contrast under the fluorescein microscope. The cell membrane of Colo201 cells was uniformly stained with anti-sialyl Le-a MAb, whereas anti-sialyl Le-x MAb only stained in the patchy areas. Pretreatment of Colo201 cells with anti-sLe-a decreased tethering, while anti-sLe-x significantly inhibited the arrest formation. Our data suggest that E-selectin alone can mediate colon cancer cell lodgement and subsequent metastasis without the contribution of integrin molecules and that the different distribution of E-selectin ligands may affect the adhesion behaviour of colon cancer cells in flow conditions.
authors.affiliation: Department of Surgery, University of Tokyo, Japan. kitayama-1SU@h.u-tokyo.ac.jp
authors.forename: J
authors.initials: J
authors.lastname: Kitayama
authors.forename: N
authors.initials: N
authors.lastname: Tsuno
authors.forename: E
authors.initials: E
authors.lastname: Sunami
authors.forename: T
authors.initials: T
authors.lastname: Osada
authors.forename: T
authors.initials: T
authors.lastname: Muto
authors.forename: H
authors.initials: H
authors.lastname: Nagawa
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D003110:Colonic Neoplasms / Q000503:physiopathology*; D019040:E-Selectin / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D019040:E-Selectin