Dihydrotestosterone prevents glucocorticoid-negative effects on fetal rat metatarsal bone in vitro.
The effects of dihydrotestosterone (DHT) on glucocorticoid-pretreated fetal rat long bone were studied in an in vitro culture system. First, dose-response curves of corticosterone, hydrocortisone, and dexamethasone were studied at several concentrations. Then, hydrocortisone (H) at 10(-5) M was selected for the second part of the study, as it slackened rudiment mineralization (104 +/- 16% of the initial dark zone vs. 141 +/- 9% in control bones), as well as its lengthening (140 +/- 4% of the harvesting day length vs. 160 +/- 1% in control bones), by both inhibition of cell proliferation and stimulation of resorption. On the contrary, in H-pretreated metatarsal bones, DHT (10(-7) M) partly limited slackening of mineralization (124 +/- 5%) and lengthening (153 +/- 2%). Moreover, a control-like cell proliferation was re-established and resorption holes were filled in. Thus, in this study, DHT partly limited hydrocortisone-induced impairment of fetal rat metatarsal bone development.
Picherit
C
C
INRA Clermont/Theix, Saint-Genès-Champanelle, France.
Coxam
V
V
Oudadesse
H
H
Martini
B
B
Gaumet
N
N
Davicco
M J
MJ
Lebecque
P
P
Miller
S
S
Irrigaray
J L
JL
Barlet
J P
JP
D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000139:chemically induced / Q000517:prevention & control; D003345:Corticosterone / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology*; D008682:Metatarsal Bones / Q000196:embryology* / Q000378:metabolism / Q000473:pathology; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar
D005938:Glucocorticoids; D013196:Dihydrotestosterone; D003907:Dexamethasone; D003345:Corticosterone; D006854:Hydrocortisone