title: Fluorescent amplified-fragment length polymorphism genotyping of Salmonella enterica subsp. enterica serovars and comparison with pulsed-field gel electrophoresis typing.
abstract: We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogen Salmonella enterica subsp. enterica comprising seven different serovars using the restriction enzymes EcoRI and MseI. From the total FAFLP fingerprinted strains, 81 were compared with pulsed-field gel electrophoresis (PFGE) typing of the same strains. The FAFLP method showed a discriminatory power equal to that of PFGE. We report a fast, robust, and high-resolution adaptation of the AFLP assay for fingerprinting S. enterica subsp. enterica serovars with capillary electrophoresis that can be scaled to high throughput on automated analysis instruments.
authors.affiliation: Department of Bacteriology, National Institute of Public Health, N-0403 Oslo, Norway. bjorn-arne.lindstedt@flokehelsa.no
authors.forename: B A
authors.initials: BA
authors.lastname: Lindstedt
authors.forename: E
authors.initials: E
authors.lastname: Heir
authors.forename: T
authors.initials: T
authors.lastname: Vardund
authors.forename: G
authors.initials: G
authors.lastname: Kapperud
mesh_terms: D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D005453:Fluorescence; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012478:Salmonella Food Poisoning / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics*