title: The effects of intraamniotic human neonatal urine and meconium on the intestines of the chick embryo with gastroschisis.
abstract: OBJECTIVE
  Urinary waste products in the amniotic fluid has been implicated as a cause of intestinal damage (ID) in gastroschisis based on the fact that fetus urinates physiologically into the amniotic cavity. However, experimental and clinical data suggest that intrauterine defecation is a physiological event, thus gastrointestinal waste products also may be responsible for ID in gastroschisis. An experimental study was performed to investigate the effects of intraamniotic human neonatal urine and diluted meconium on the intestines of chick embryo with gastroschisis.
  
  
  METHODS
  Five-day-old fertilized chick eggs (Gallus domesticus) were used. Gastroschisis was created through amniotic cavity without opening the allantoic cavity. Sterile urine and meconium were obtained from newborn humans, and 1% meconium suspension was prepared. The eggs were divided in to 3 groups. In the first group, gastroschisis was created, and amniotic fluid was reinstilled without changing its composition (control group). Equal amounts of amniotic fluid and urine mixture was instilled into the amniotic cavity in second group (urine group) and 1% meconium suspension was instilled in similar fashion in the third group after creation of gastroschisis (meconium group).
  
  
  RESULTS
  Histopathologic features of the intestines of the urine group did not differ from the intestines of the control group. The meconium group's bowel showed serosal thickening, inflammation, focal fibrin, and collagen deposits. Histopathologic changes of intestines induced by intraamniotic diluted meconium are consistent with the ones described for human gastroschisis specimens.
  
  
  CONCLUSIONS
  Gastrointestinal waste products seem responsible for the ID in gastroschisis rather than urinary waste products.
authors.affiliation: Department of Pediatric Surgery, Dokuz Eylül University Medical School, Izmir, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Olguner
authors.forename: F M
authors.initials: FM
authors.lastname: Akgür
authors.forename: A
authors.initials: A
authors.lastname: Api
authors.forename: E
authors.initials: E
authors.lastname: Ozer
authors.forename: T
authors.initials: T
authors.lastname: Aktuğ
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D002642:Chick Embryo; D020139:Gastroschisis / Q000473:pathology*; D006801:Humans; D007422:Intestines / Q000473:pathology*; D008470:Meconium; D051381:Rats; D014556:Urine