Antibody responses of goldfish (Carassius auratus L.) to DNA-immunisation at different temperatures and feeding levels.
DNA vaccines which work in winter are needed for fish. At 18 weeks after intramuscular injection of a plasmid containing lacZ goldfish at 15 degrees C had five-fold more antibody to betagalactosidase than those at 25 degrees C or those at </=9 degrees C. Expression persisted for 1 week at 25 degrees C and for 4 weeks at 15 degrees C. Expression was highest after 18 weeks at </=9 degrees C and was correlated to antibody titre in this group. Goldfish on low or high rations (0.33 or 3% body weight daily) made the same amount of antibody whilst losing 20% or gaining 50% of their body-weight over 2 months at 22 degrees C.
Russell
P H
PH
Department of Pathology, The Royal Veterinary College, Royal College Street, London, UK. prussell@rvc.ac.uk
Kanellos
T
T
Negrou
M
M
Ambali
A A
AA
D000818:Animals; D000917:Antibody Formation; D004435:Eating; D015870:Gene Expression; D006054:Goldfish / Q000276:immunology*; D007273:Injections, Intramuscular; D007763:Lac Operon; D018485:Muscle Fibers, Skeletal / Q000201:enzymology; D010957:Plasmids / Q000235:genetics; D012621:Seasons; D013696:Temperature; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology
D019444:Vaccines, DNA; D001616:beta-Galactosidase