[The role of topoisomerase II in mammalian cells response to ionizing radiation. II. Effect of a small concentration of novobiocin on cell cycle in x-rays irradiated HeLa cells].
Flow cytometry was used to study cell cycle recovery in X-irradiated HeLa cells after the action of novobiocin, an inhibitor of topoisomerase II. A prolonged treatment with 0.4 mM novobiocin (30 h) of intact cells did not affect cell cycle progression. Novobiocin treatment of 5 Gy-irradiated cells resulted in a significant decrease of G2-block, if compared with X-irradiated cells. These data, together with our previous results on the effect of 1 mM novobiocin on cell cycle in X-irradiated CHO K1 cells, allow to suggest an involvement of topoisomerase II in cell response to ionizing radiation. But one cannot exclude a concurrent effect of novobiocin with proteins other than topoisomerase II, for example, with protein p34cdc2-kinase, known to be involved in cell cycle regulation.
Kirrilova
T V
TV
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006367:HeLa Cells / Q000166:cytology* / Q000201:enzymology / Q000528:radiation effects*; D006801:Humans; D009675:Novobiocin / Q000494:pharmacology*; D059005:Topoisomerase II Inhibitors; D014965:X-Rays
D004791:Enzyme Inhibitors; D059005:Topoisomerase II Inhibitors; D009675:Novobiocin; D004250:DNA Topoisomerases, Type II