Restriction fragment length polymorphism analysis of Mycobacterium avium isolates from animal and human sources.
Restriction fragment length polymorphism (RFLP) analysis using probes derived from the insertion sequences IS901, IS1245 and IS1311, was carried out on Mycobacterium avium isolates obtained from 18 human patients, 44 deer, 14 pigs and five cattle in the Republic of Ireland. Forty-two of the cervine isolates and two of the bovine isolates contained IS901, while this insertion sequence was absent from all of the human and porcine isolates. RFLP analysis with IS901 probe differentiated the 44 field isolates which contained this element into three types. All of the IS901-positive isolates had a characteristic three-band IS1245 hybridisation pattern and a characteristic single-band IS1311 hybridisation pattern. The IS901-negative isolates exhibited highly polymorphic IS1245 and IS1311 hybridisation patterns which differentiated the human and porcine isolates into a wide diversity of strain types.
O'Grady
D
D
Central Veterinary Research Laboratory, Abbotstown, Castleknock, Ireland. eamoncos@indigo.ie
Flynn
O
O
Costello
E
E
Quigley
F
F
Gogarty
A
A
McGuirk
J
J
O'Rourke
J
J
Gibbons
N
N
D000818:Animals; D002417:Cattle; D016172:DNA Fingerprinting; D003670:Deer; D006801:Humans; D009162:Mycobacterium avium / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D013552:Swine