title: A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria.
abstract: A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required.
authors.affiliation: Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.
authors.forename: J
authors.initials: J
authors.lastname: McKerrow
authors.forename: S
authors.initials: S
authors.lastname: Vagg
authors.forename: T
authors.initials: T
authors.lastname: McKinney
authors.forename: E M
authors.initials: EM
authors.lastname: Seviour
authors.forename: A M
authors.initials: AM
authors.lastname: Maszenan
authors.forename: P
authors.initials: P
authors.lastname: Brooks
authors.forename: R J
authors.initials: RJ
authors.lastname: Seviour
mesh_terms: D002473:Cell Wall / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003960:Diaminopimelic Acid / Q000032:analysis*; D006094:Gram-Positive Bacteria / Q000737:chemistry*
chemical_list: D003960:Diaminopimelic Acid