[Effects of anti-inflammatory drugs on adhesion and migration of lymphocytes].
The first crucial step of cell recruitment to the sites of inflammation is adhesion and motility, which are interdependent phenomena. The purpose of our study was investigate the influence used anti-inflammatory drugs (Aspirin, Cyclosporin A, Methotrexate) and endogenous substances of anti-inflammatory activity (Cortisol, TGF-beta) on these phenomena. Lymphocytes from healthy subjects were preincubated with various concentrations of these substances and adhesion to plastic bound CD11a/CD18, CD11c/CD18, CD44 and CD62L mAbs was determined. Simultaneously, cell motility was investigated after Boyden method. We found significant reduction of cell adhesion to all the ligands used. Furthermore we observed significant dose dependent increase in motility of these cells. These data indicate that simultaneous enhancement of motility and reduction of adhesion might be a common pathway for the mechanisms of action of anti-inflammatory agents.
Zak-Nejmark
T
T
Katedra i Klinika Chorób Wewnetrznych i Alergologii AM we Wrocławiu.
Kraus-Filarska
M
M
Małolepszy
J
J
Jankowska
R
R
Nadobna
G
G
Nowak
I
I
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015703:Antigens, CD / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008727:Methotrexate / Q000494:pharmacology; D012016:Reference Values; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D015703:Antigens, CD; D016212:Transforming Growth Factor beta; D016572:Cyclosporine; D001241:Aspirin; D006854:Hydrocortisone; D008727:Methotrexate