Immunoglobulin kappa light-chain V, J, and C gene sequences of the owl monkey Aotus nancymaae.
Sequences of Aotus nancymaae immunoglobulin kappa light-chain rearrangements were analyzed after reverse transcription polymerase chain reaction. Among 22 in-frame rearrangements analyzed, 12 IGKV genes belonging to the families 1, 2, or 3 were identified. Aotus counterparts for all five human IGKJ genes were found. The identity of the deduced human and Aotus amino acid sequences was between 83% and 92% for junctional regions and 74% for the constant region. Sequence comparisons between rearrangements indicated that somatic mutations, the addition of nongermline-encoded nucleotides, and exonuclease trimming contribute to the generation of diversity of Aotus immunoglobulin kappa chains. The high identity of Aotus and human IGK genes is comparable to that of T-cell receptor genes and further supports the proposal to use the Aotus Plasmodium falciparum infection model for the evaluation of malaria vaccine candidates.
Diaz
O L
OL
Instituto de Inmunologia, Hospital San Juan de Dios, Santafe de Bogota, Colombia.
Daubenberger
C A
CA
Rodriguez
R
R
Naegeli
M
M
Moreno
A
A
Patarroyo
M E
ME
Pluschke
G
G
D000595:Amino Acid Sequence; D000818:Animals; D016645:Aotidae / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D018076:DNA, Complementary; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D007127:Immunoglobulin Constant Regions; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains