title: Human osteoblast-like cells phagocytose metal particles and express the macrophage marker CD68 in vitro.
abstract: Periprosthetic osteolysis is a major cause of aseptic loosening in artificial joint replacement. It is assumed to occur in conjunction with the activation of macrophages. We have shown in vitro that human osteoblast-like cells, isolated from bone specimens obtained from patients undergoing hip replacement, phagocytose fine particles of titanium alloy (TiAlV). The human osteoblast-like cells were identified immunocytochemically by the presence of bone-specific alkaline phosphatase (BAP). With increasing duration of culture, a variable number of the osteoblastic cells became positive for the macrophage marker CD68, independent of the phagocytosis of particles, with a fine granular cytoplasmic staining which was coexpressed with BAP as revealed by immunodoublestaining. The metal particles were not toxic to the osteoblastic cells since even in culture for up to four weeks massively laden cells were vital and had a characteristic morphology. Cells of the human osteosarcoma cell line (HOS 58) were also able to phagocytose metal particles but had only a low expression of the CD68 antigen. Fluorescence-activated cell scanning confirmed our immunocytochemical results. Additionally, the cells were found to be negative for the major histocompatibility complex-II (MHC-II) which is a marker for macrophages and other antigen-presenting cells. Negative results of histochemical tests for tartrate-resistant acid phosphatase excluded the contamination by osteoclasts or macrophages in culture. Our observations suggest that the osteoblast can either change to a phagocytosing cell or that the phagocytosis is an underestimated property of the osteoblast. The detection of the CD68 antigen is insufficient to prove the monocytic lineage. In order to discriminate between macrophages and osteoblasts additional markers should be used. To our knowledge, this is the first demonstration of cells of an osteoblastic origin which have acquired a mixed phenotype of both osteoblasts and macrophages.
authors.affiliation: Department of Orthopaedics, Georg-August-University, Goettingen, Germany.
authors.forename: D E
authors.initials: DE
authors.lastname: Heinemann
authors.forename: C
authors.initials: C
authors.lastname: Lohmann
authors.forename: H
authors.initials: H
authors.lastname: Siggelkow
authors.forename: F
authors.initials: F
authors.lastname: Alves
authors.forename: I
authors.initials: I
authors.lastname: Engel
authors.forename: G
authors.initials: G
authors.lastname: Köster
mesh_terms: D000368:Aged; D000497:Alloys; D015703:Antigens, CD / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D008262:Macrophage Activation / Q000502:physiology; D008264:Macrophages / Q000648:ultrastructure*; D008875:Middle Aged; D010006:Osteoblasts / Q000648:ultrastructure*; D012516:Osteosarcoma; D010587:Phagocytosis / Q000502:physiology*; D011475:Prosthesis Failure; D014025:Titanium; D014407:Tumor Cells, Cultured / Q000648:ultrastructure; D000097382:CD68 Molecule
chemical_list: D000497:Alloys; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D014025:Titanium; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C031462:titanium alloy (TiAl6V4)