Medium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the gamma-decalactone production by Yarrowia lipolytica.
Size of methyl ricinoleate droplets during biotransformation into gamma-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium, droplets had an average volume surface diameter d32 of 2.5 microm whereas it was 0.7 microm in homogenized and shaken medium. But as soon as yeast cells were inoculated, both diameters became similar at about 0.7 microm and did not vary significantly until the end of the culture. The growth of Y. lipolytica in both media was very similar except for the lag phase which was lowered in homogenized medium conditions.
Waché
Y
Y
Laboratoire de Biotechnologie and Laboratoire de Biochimie alimentaire, ENSBANA-Université de Bourgogne, Dijon and Laboratoire de Génétique des Microorganismes, INRA-CNRS, Thiverval-Grignon, France. ywache@u-bourgogne.fr
Bergmark
K
K
Courthaudon
J L
JL
Aguedo
M
M
Nicaud
J M
JM
Belin
J M
JM
D001711:Biotransformation; D003470:Culture Media; D007783:Lactones / Q000378:metabolism*; D018613:Microscopy, Confocal; D010316:Particle Size; D012277:Ricinoleic Acids / Q000378:metabolism*; D013997:Time Factors; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D007783:Lactones; D012277:Ricinoleic Acids; C109757:decan-4-olide; C005235:methyl ricinoleate