Zona pellucida autoantibodies in women undergoing ART.
The presence of ZP autoantibodies in serum and Ffl samples of 109 women attending the ART Center in Kiev was investigated using IIF and ELISA. Positive serum and Ffl samples examined by both methods were found in 20 (18.34%) and in 19 (17.43%) patients respectively; 31 (28.44%) serum samples and 33 (30.27 %) Ffl samples analyzed by IIF were positive; of the samples analyzed by ELISA 21 (19.26%) and 20 (18.34%), respectively, were positive. No relationship was found between ZP autoantibody incidence and the type and cause of infertility. A significant prevalence of ZP autoantibodies detected by ELISA in Ffl was found in patients with fertilization failure (39.13%) and with low fertilization rate (42.85%) when compared to patients with middle fertilization rate (5.71%) and high fertilization rate (8.1%). The clinical significance of ZP autoantibodies in Ffls for in vitro fertilization outcome was suggested.
Ivanova
M
M
Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia.
Djarkova
T
T
Mollova
M
M
Petrov
M
M
Tikhomirova
T
T
Dakhno
F
F
D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001826:Body Fluids / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005306:Fertilization / Q000276:immunology*; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000276:immunology* / Q000628:therapy; D006080:Ovarian Follicle / Q000276:immunology; D015044:Zona Pellucida / Q000276:immunology*
D001323:Autoantibodies