title: Interstitial fluid pressure is increased in renal cell carcinoma xenografts.
abstract: The purpose of this study was to test the hypothesis that renal cell carcinoma (RCC) exhibits an increased intratumoral interstitial fluid pressure (IT-IFP). Therefore, resected tumors from human primary (n = 23) or metastatic RCC (n = 3) were xenografted in SCID mice. The IFP of single tumor nodules (n = 65) and normal mouse tissue (n = 195) was measured by means of the "wick-in-needle" technique. Data demonstrate that the mean IT-IFP at neoplasia was 35 times greater than in normal tissue, and decreased precipitously at the tumor boundary. IT-IFP values tended to increase with the grade of malignancy of the tumor cells and tumor size. The mean IT-IFP of xenografts derived from primary RCC was twice as high as that from metastatic RCC tissue. These findings indicate a biophysical barrier to drug delivery in RCC; this may, in concert with cellular-based drug resistance mechanisms, be an additional explanation for resistance of the tumor to certain blood-borne anticancer therapies.
authors.affiliation: Department of Urology, Medical Faculty, University of Aachen, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Rohde
authors.forename: C
authors.initials: C
authors.lastname: Wiesner
authors.forename: D
authors.initials: D
authors.lastname: Graf
authors.forename: J
authors.initials: J
authors.lastname: Wolff
authors.forename: L
authors.initials: L
authors.lastname: Füzesi
authors.forename: G
authors.initials: G
authors.lastname: Jakse
mesh_terms: D000818:Animals; D002292:Carcinoma, Renal Cell / Q000503:physiopathology* / Q000556:secondary; D005110:Extracellular Space / Q000502:physiology*; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000503:physiopathology*; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011312:Pressure; D014183:Transplantation, Heterologous