Identification of a 49-kDa hydrophobic cell wall mannoprotein present in velum yeast which may be implicated in velum formation.
Analysis of velum-forming yeast cell wall components released by beta-1,3-glucanase treatment were compared with those of a non velum-forming yeast. SDS-PAGE electrophoresis and Western blotting with ConA-peroxidase staining of mannoproteins allowed us to identify a 49-kDa mannoprotein present in the cell wall of the velum-forming yeast and hardly visible in the control. The cell wall nature of this protein was confirmed by labelling with the non-permeable sulfosuccinimydiyl-6-(biotinamido)hexanoate reagent. A partial purification of this mannoprotein by anion exchange HPLC followed by surface hydrophobicity determination revealed that the fraction containing the 49-kDa mannoprotein was the most hydrophobic. Since cell surface hydrophobicity plays an important role in aggregate formation, it is likely that this mannoprotein is involved in velum formation.
Alexandre
H
H
Laboratoire d'oenologie, Institut Jules Guyot, Université de Bourgogne, BP 27877, 21078, Dijon, France. rvalex@u-bourgogne.fr
Blanchet
S
S
Charpentier
C
C
D019921:Biotinylation; D015153:Blotting, Western; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D013499:Surface Properties; D014920:Wine / Q000382:microbiology*
D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C025363:mannoproteins