A new method for direct introduction of chemicals into a single sieve tube of intact rice plants.
We present a novel method for direct introduction of chemicals into a sieve tube of intact rice plants-namely, the method of micro-introduction using stylet of insect (the MUSI method). Fluorescent dyes were successfully introduced into a sieve tube through a severed stylet of planthopper and the distribution of the dyes was observed.
Fujimaki
S
S
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. aa67029@mail.ecc.u-tokyo.ac.jp
Fujiwara
T
T
Hayashi
H
H
D000818:Animals; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007313:Insecta; D008845:Microinjections / Q000379:methods; D008856:Microscopy, Fluorescence; D012275:Oryza / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D012235:Rhodamines / Q000008:administration & dosage / Q000493:pharmacokinetics
D005456:Fluorescent Dyes; D012235:Rhodamines; C005358:tetramethylrhodamine; D016650:Fluorescein-5-isothiocyanate