title: The steady-state levels of oxidative DNA damage and of lipid peroxidation (F2-isoprostanes) are not correlated in healthy human subjects.
abstract: Oxidative damage to DNA in human tissues can be determined by measuring multiple products of oxidative damage to the purine and pyrimidine bases using gas chromatography-mass spectrometry (GC-MS). Oxidative damage to lipids (lipid peroxidation) can be quantitated by the mass spectrometry-based determination of F2-isoprostanes, specific end-products of the peroxidation of arachidonic acid residues in lipids. For both DNA base damage products and 8-epi prostaglandin F2alpha (PGF2alpha), there is a wide variation in levels between different healthy human subjects. We measured multiple products of oxidative damage to DNA bases in white cells, and 8-epi PGF2alpha in plasma, from blood samples obtained from healthy human subjects in the UK and in Portugal. No correlation of 8-epi PGF2alpha levels with levels of any modified DNA base (including 8-hydroxyguanine) was observed. We conclude that no single parameter can be measured as an index of "oxidative stress" or "oxidative damage" in vivo.
authors.affiliation: International Antioxidant Research Centre, King's College, Guys Campus, London, UK.
authors.forename: T
authors.initials: T
authors.lastname: England
authors.forename: E
authors.initials: E
authors.lastname: Beatty
authors.forename: A
authors.initials: A
authors.lastname: Rehman
authors.forename: J
authors.initials: J
authors.lastname: Nourooz-Zadeh
authors.forename: P
authors.initials: P
authors.lastname: Pereira
authors.forename: J
authors.initials: J
authors.lastname: O'Reilly
authors.forename: H
authors.initials: H
authors.lastname: Wiseman
authors.forename: C
authors.initials: C
authors.lastname: Geissler
authors.forename: B
authors.initials: B
authors.lastname: Halliwell
mesh_terms: D000328:Adult; D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011174:Portugal; D006113:United Kingdom
chemical_list: C075750:8-epi-prostaglandin F2alpha; D016718:Arachidonic Acid; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; D015237:Dinoprost