title: Promotion of hepatocarcinogenesis by hexachlorobenzene in energy-restricted rats.
abstract: The interaction between dietary energy restriction and low dose of the fungicide hexachlorobenzene (HCB) was evaluated in a rat liver medium-term bioassay for carcinogenesis. Male Wistar rats were fed a control or a 50% energy-restricted diet, both added or not with 50 ppm HCB, for 6 weeks. HCB exposure or energy restriction separately did not exert any influence on the development of glutathione S-transferase placental form (GST-P(+)) foci of hepatocytes. Simultaneous HCB exposure and energy restriction induced a significant increase in liver centrilobular hypertrophy and GST-P(+) foci development. Our findings suggest that energy restriction increases liver response to low dose of HCB, unmasking the promoting potential of this fungicide.
authors.affiliation: Departamento de Patologia Aplicada, Legislação e Deontologia, Faculdade de Medicina, UEL, Londrina, PR, Brazil.
authors.forename: M O
authors.initials: MO
authors.lastname: Kishima
authors.forename: L F
authors.initials: LF
authors.lastname: Barbisan
authors.forename: D
authors.initials: D
authors.lastname: Estevão
authors.forename: M A
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: J L
authors.initials: JL
authors.lastname: Viana de Camargo
mesh_terms: D000818:Animals; D001835:Body Weight; D003043:Cocarcinogenesis; D002149:Energy Intake; D005659:Fungicides, Industrial / Q000633:toxicity; D006581:Hexachlorobenzene / Q000633:toxicity*; D008099:Liver / Q000033:anatomy & histology / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D008297:Male; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005659:Fungicides, Industrial; D006581:Hexachlorobenzene