Formate-induced inhibition of the water-oxidizing complex of photosystem II studied by EPR.
The effects of various formate concentrations on both the donor and the acceptor sides in oxygen-evolving PS II membranes (BBY particles) were examined. EPR, oxygen evolution and variable chlorophyll fluorescence have been observed. It was found that formate inhibits the formation of the S(2) state multiline signal concomitant with stimulation of the Q(A)(-)Fe(2+) signal at g = 1.82. The decrease and the increase in intensities of the multiline and Q(A)(-)Fe(2+) signals, respectively, had a linear relation for formate concentrations between 5 and 500 mM. The g = 4.1 signal formation measured in the absence of methanol was not inhibited by formate up to 250 mM in the buffer. In the presence of 3% methanol the g = 4.1 signal evolved as formate concentration increased. The evolved signal could be ascribed to the inhibited centers. Oxygen evolution measured in the presence of an electron acceptor, phenyl-p-benzoquinone, was also inhibited by formate proportionally to the decrease in the multiline signal intensity. The inhibition seemed to be due to a retarded electron transfer from the water-oxidizing complex to Y(Z)(+), which was observed in the decay kinetics of the Y(Z)(+) signal induced by illumination above 250 K. These results show that formate induces inhibition of water oxidation reactions as well as electron transfer on the PS II acceptor side. The inhibition effects of formate in PS II were found to be reversible, indicating no destructive effect on the reaction center induced by formate.
Feyziev
Y M
YM
Institute of Botany, Academy of Sciences, Patamdar shosse 40, Baku 370073, Azerbaijan, Japan.
Yoneda
D
D
Yoshii
T
T
Katsuta
N
N
Kawamori
A
A
Watanabe
Y
Y
D002736:Chloroplasts / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005561:Formates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000737:chemistry; D007700:Kinetics; D045342:Light-Harvesting Protein Complexes; D010084:Oxidation-Reduction; D010100:Oxygen / Q000037:antagonists & inhibitors / Q000737:chemistry; D045322:Photosynthetic Reaction Center Complex Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D045332:Photosystem II Protein Complex; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D005561:Formates; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; C114979:tyrosyl radical YZ; D014867:Water; C030544:formic acid; D014443:Tyrosine; D010100:Oxygen