[Antitumor and immunological activities of oxalysine].
OBJECTIVE
To study the antitumor and immunological activities of oxalysine (OXL).


METHODS
Growth of mouse ascites hepatoma-22 (H-22) cells and splenocyte proliferation were investigated in vitro by MTT colorimetric assay.


RESULTS
Suppression of OXL (6.3-200 micrograms/ml) on H-22 cell growth was weaker when used singly. But a marked synergetic inhibition was observed when OXL was used in combination with 5-fluorouracil (5-Fu), with suppression percentage on hepatoma growth increased to 43.1% from 13.8% (25 micrograms/ml of OXL alone) or 18.9% (3.1 micrograms/ml of 5-Fu alone). OXL (6.3-100 micrograms/ml) in vitro produced an inhibitory action on splenocyte proliferation induced by concanvilian A (Con A, 5 micrograms/ml) in the normal mice, but was able to enhance obviously the proliferative response in mice bearing H-22.


CONCLUSIONS
OXL inhibits H-22 cell growth. The immunoregulatory action of OXL may contribute to its antihepatoma activity.
Wang
X
X
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Xu
B
B
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000600:Amino Acids, Dicarboxylic / Q000502:physiology; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D005260:Female; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D013154:Spleen / Q000166:cytology*; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D000600:Amino Acids, Dicarboxylic; D000903:Antibiotics, Antineoplastic; C027136:4-oxalysine