[The use of in situ polymerase chain reaction for detection the intracellular localization of Mycoplasma hominis in cultured HeLa cells].
Mycoplasma hominis was incubated with HeLa cells up to 72 h. Infection of HeLa cells was estimated by in situ polymerase chain reaction with a subsequent use of computer digital image analysis for result processing. The localization of mycoplasma in the cell nucleus was demonstrated after a 48 h incubation. The quantity of infected HeLa cells was increased with time to make 58 and 75% on days 2 and 3, respectively. No M. hominis was found in the cytoplasma of HeLa cells.
Momynaliev
K T
KT
Research Institute of Physico-Chemical Medicine, Ministry of Public Health, Russian Federation, Moscow.
Orlova
V F
VF
Zhukotskiĭ
A V
AV
Kogan
E M
EM
Govorun
V M
VM
D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D019535:Mycoplasma hominis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity