title: mtDNA tandem repeats in domestic dogs and wolves: mutation mechanism studied by analysis of the sequence of imperfect repeats.
abstract: The mitochondrial (mt) DNA control region (CR) of dogs and wolves contains an array of imperfect 10 bp tandem repeats. This region was studied for 14 domestic dogs representing the four major phylogenetic groups of nonrepetitive CR and for 5 wolves. Three repeat types were found among these individuals, distributed so that different sequences of the repeat types were formed in different molecules. This enabled a detailed study of the arrays and of the mutation events that they undergo. Extensive heteroplasmy was observed in all individuals; 85 different array types were found in one individual, and the total number of types was estimated at 384. Among unrelated individuals, no identical molecules were found, indicating a high rate of evolution of the region. By performing a pedigree analysis, array types which had been inherited from mother to offspring and array types which were the result of somatic mutations, respectively, could be identified, showing that about 20% of the molecules within an individual had somatic mutations. By direct pairwise comparison of the mutated and the original array types, the physiognomy of the inserted or deleted elements (indels) and the approximate positions of the mutations could be determined. All mutations could be explained by replication slippage or point mutations. The majority of the indels were 1-5 repeats long, but deletions of up to 17 repeats were found. Mutations were found in all parts of the arrays, but at a higher frequency in the 5' end. Furthermore, the inherited array types within the mother-offspring pair were aligned and compared so that germ line mutations could be studied. The pattern of the germ line mutations was approximately the same as that of the somatic mutations.
authors.affiliation: Department of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Savolainen
authors.forename: L
authors.initials: L
authors.lastname: Arvestad
authors.forename: J
authors.initials: J
authors.lastname: Lundeberg
mesh_terms: D000818:Animals; D004247:DNA / Q000097:blood; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D019143:Evolution, Molecular; D017354:Point Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences / Q000235:genetics*; D019596:Wolves / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D004247:DNA