title: A new HLA-A*02 allele, A*0234, detected by polymerase chain reaction using sequence-specific primers (PCR-SSP).
abstract: A novel HLA-A*02 allele, A*0234, was identified in a potential unrelated bone marrow donor typed by polymerase chain reaction using sequence-specific primers (PCR-SSP). Positive reactions obtained upon testing with PCR-SSP did not fit any known combination of alleles indicating the possible presence of a novel allele. Sequencing of clones from this individual revealed the presence of a novel allele, HLA-A*0234. The sequence of exons 2, 3 and part of exon 4 showed that A*0234 differed from A*02011 by a single nucleotide in exon 2 at position 282 (C to G). The nucleotide substitution results in an amino acid change at residue 70 (Histidine to Glutamine) in the alpha1 domain.
authors.affiliation: Tissue Typing Services, Australian Red Cross Blood Service, North East Region, Sydney, NSW. jmoses@arcbs.redcross.org.au
authors.forename: J H
authors.initials: JH
authors.lastname: Moses
authors.forename: W D
authors.initials: WD
authors.lastname: Greville
authors.forename: J
authors.initials: J
authors.lastname: Downes
authors.forename: W
authors.initials: W
authors.lastname: McClenahan
authors.forename: A
authors.initials: A
authors.lastname: Kennedy
authors.forename: H
authors.initials: H
authors.lastname: Dunckley
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D015789:HLA-A2 Antigen / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D015789:HLA-A2 Antigen