title: Clathrin coat construction in endocytosis.
abstract: Electron cryomicroscopy of the clathrin coat and X-ray crystallography of parts of the clathrin heavy chain combine to give a detailed picture of the clathrin molecule, assembled as a cage. Recently determined domain structures of other components of the endocytic machinery, particularly the mu2 subunit and the alpha-appendage domain of the AP2 adaptor complex, provide important information on the sequence of recognition events involved in the dynamic process of clathrin coat assembly.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, CB2 2QH, UK. bulbeck@mrc-lmb.cam.acuk
authors.forename: B M
authors.initials: BM
authors.lastname: Pearse
authors.forename: C J
authors.initials: CJ
authors.lastname: Smith
authors.forename: D J
authors.initials: DJ
authors.lastname: Owen
mesh_terms: D033961:Adaptor Protein Complex 1; D033962:Adaptor Protein Complex 2; D033963:Adaptor Protein Complex 3; D033965:Adaptor Protein Complex alpha Subunits; D033983:Adaptor Protein Complex mu Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D002966:Clathrin / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D003034:Coated Pits, Cell-Membrane / Q000737:chemistry*; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000502:physiology; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: C499064:AP1M2 protein, human; C510070:AP3M2 protein, human; D033961:Adaptor Protein Complex 1; D033962:Adaptor Protein Complex 2; D033963:Adaptor Protein Complex 3; D033965:Adaptor Protein Complex alpha Subunits; D033983:Adaptor Protein Complex mu Subunits; D033942:Adaptor Proteins, Vesicular Transport; D002966:Clathrin; D008565:Membrane Proteins; C499065:adaptor protein complex 1, mu 2 subunit; C438886:adaptor protein complex 2, mu 2 subunit