Properties of a polyamine transporter regulated by antizyme.
The regulation of polyamine transport by antizyme, a protein that is involved in the rapid degradation of ornithine decarboxylase (ODC), was studied in FM3A mouse cells overproducing ODC. Both artificial (Z1) and natural antizymes not only inhibited polyamine uptake but also stimulated polyamine excretion. The properties of the polyamine transporter regulated by antizyme were characterized. The uptake of radiolabelled polyamines was inhibited by excess acetylpolyamines and a protonophore, CCCP (carbonyl cyanide m-chlorophenylhydrazone), whereas the excretion of radiolabelled polyamines was stimulated by unlabelled polyamines, acetylpolyamines and CCCP in the medium. Furthermore, it is shown that polyamines and acetylpolyamines are excreted from cells. On the basis of the results, it is discussed how antizyme regulates polyamine transport negatively.
Sakata
K
K
Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
Kashiwagi
K
K
Igarashi
K
K
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D007202:Indicators and Reagents; D007700:Kinetics; D051379:Mice; D009861:Onium Compounds / Q000493:pharmacokinetics; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D014162:Transfection
D002352:Carrier Proteins; D007202:Indicators and Reagents; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D011073:Polyamines; D011506:Proteins; D011994:Recombinant Proteins; C031017:ornithine decarboxylase antizyme; D013096:Spermine; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D009955:Ornithine Decarboxylase; D013095:Spermidine; C013289:tetraphenylphosphonium; D011700:Putrescine