title: Localization of mRNAs for phospholipase D (PLD) type 1 and 2 in the brain of developing and mature rat.
abstract: Phospholipase D (PLD) is known as one of the key enzymes in the lipid metabolism which produces several second messengers. We demonstrated the localization of the gene expression for PLD1 and PLD2 using in situ hybridization histochemistry in the brain of developing and mature rats. Whereas PLD1 mRNA expression was detected mainly in presumptive oligodendrocytes, PLD2 mRNA expression was detected mainly in presumptive astrocytes. In addition, the gene expression for PLDs were expressed in neuroepithelial cells of the ventricular/ependymal zones and the gene for PLD2 was expressed transiently in early postnatal gray matters, presumptive neurons.
authors.affiliation: Division of Histology, Department of Cell Biology, Graduate School of Medical Sciences, Tohoku University, 2-1, Seiryo-machi, Aoba-ku, Sendai, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Sakagami
authors.forename: H
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000367:Age Factors; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D009457:Neuroglia / Q000201:enzymology; D010713:Phosphatidylcholines / Q000378:metabolism; D010739:Phospholipase D / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010713:Phosphatidylcholines; D012333:RNA, Messenger; C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1