Hematopoiesis in larval Pseudoplusia includens and Spodoptera frugiperda.
Maintenance of circulating hemocytes in larval Lepidoptera has been attributed to both mitosis of hemocytes already in circulation and the release of hemocytes from hematopoietic organs. In this study, we compared hematopoiesis in the noctuids Pseudoplusia includens and Spodoptera frugiperda. For both species, hemocyte densities per microl of blood increased with instar. Differential hemocyte counts indicated that plasmatocytes were the most abundant hemocyte type during early instars but granular cells were the most abundant hemocyte type in the last instar. Hematopoietic organs were located in the meso- and metathorax of S. Frugiperda and P. Includens. These organs contained large numbers of hemocytes in S. Frugiperda, but contained few hemocytes in P. Includens. The majority of the hemocytes recovered from hematopoietic organs were identified as plasmatocytes. Using hemocyte type-specific markers and bromodeoxyuridine (BrdU) incorporation experiments, we determined that all hemocyte types with the exception of oenocytoids synthesize DNA. BrdU labeling indices for both species also fluctuated with the molting cycle. Ligation experiments suggested that hematopoietic organs are an important source of circulating plasmatocytes in S. Frugiperda but not in P. Includens. Injection of heat killed bacteria into larvae induced higher levels of BrdU labeling than injection of sterile saline, suggesting that infection and wounding induce different levels of hemocyte proliferation. Arch.
Gardiner
E M
EM
Department of Entomology, 237 Russell Laboratories, University of Wisconsin-Madison, 53706, USA.
Strand
M R
MR
D000818:Animals; D016590:Aphidicolin / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count; D004926:Escherichia coli; D006410:Hematopoiesis; D006434:Hemocytes; D007267:Injections; D007814:Larva / Q000254:growth & development; D009036:Moths / Q000254:growth & development; D018411:Spodoptera / Q000254:growth & development
D016590:Aphidicolin; D001973:Bromodeoxyuridine