title: Alpha-tocopherol dietary supplement decreases titers of antibody against 5-hydroxymethyl-2'-deoxyuridine (HMdU).
abstract: This study evaluated the effects of vitamin E (alpha-tocopherol) on oxidative DNA damage in a randomized double-blind Phase II chemoprevention trial. Oxidative DNA damage was measured by the level of auto-antibody (Ab) against 5-hydroxymethyl-2'-deoxyuridine (HMdU) in plasma. After the baseline screening, eligible subjects (n = 31; plasma samples from 28 subjects were available for this study) were randomized to receive 15, 60, or 200 mg of alpha-tocopherol per day for 28 days. Biomarkers were measured twice at baseline--on day 1 (visit 1) and day 3 (visit 2)--and twice after intervention--on day 17 (visit 3) and day 31 (visit 4). At baseline, there was a highly significant inverse correlation between anti-HMdU Ab titer and plasma vitamin E level (r = -0.53; P = 0.004; n = 28). Smoking did not affect baseline anti-HMdU Ab titer; however, anti-HMdU Ab titer levels at baseline were significantly lower in subjects with above-median (0.75 ounce/day) alcohol consumption (P = 0.008). No significant change in anti-HMdU Ab level occurred at either visit 3 or visit 4 for subjects on the lowest dose, 15 mg alpha-tocopherol per day. Subjects receiving 60 mg of alpha-tocopherol per day had a significant decrease in anti-HMdU Ab level at visits 3 and 4 compared with baseline (P = 0.049 and P = 0.02, respectively). However, subjects receiving the highest dose, 200 mg/day, had less consistent results: a significant decrease in anti-HMdU Ab level was seen at visit 4 (P = 0.04) but not at visit 3. Our results demonstrate an inverse relationship between alpha-tocopherol and anti-HMdU Abs in plasma; oxidative DNA damage can be modulated by short-term dietary supplementation of alpha-tocopherol in some subjects.
authors.affiliation: Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20007, USA. jenhu@wfubmc.edu
authors.forename: J J
authors.initials: JJ
authors.lastname: Hu
authors.forename: C X
authors.initials: CX
authors.lastname: Chi
authors.forename: K
authors.initials: K
authors.lastname: Frenkel
authors.forename: B N
authors.initials: BN
authors.lastname: Smith
authors.forename: J J
authors.initials: JJ
authors.lastname: Henfelt
authors.forename: M
authors.initials: M
authors.lastname: Berwick
authors.forename: S
authors.initials: S
authors.lastname: Mahabir
authors.forename: R B
authors.initials: RB
authors.lastname: D'Agostino
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D004249:DNA Damage; D004260:DNA Repair; D003857:Deoxyuridine / Q000031:analogs & derivatives*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000517:prevention & control; D017382:Reactive Oxygen Species; D014810:Vitamin E / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D001323:Autoantibodies; D017382:Reactive Oxygen Species; D014810:Vitamin E; D003857:Deoxyuridine