A detailed linkage map of medaka, Oryzias latipes: comparative genomics and genome evolution.
We mapped 633 markers (488 AFLPs, 28 RAPDs, 34 IRSs, 75 ESTs, 4 STSs, and 4 phenotypic markers) for the Medaka Oryzias latipes, a teleost fish of the order Beloniformes. Linkage was determined using a reference typing DNA panel from 39 cell lines derived from backcross progeny. This panel provided unlimited DNA for the accumulation of mapping data. The total map length of Medaka was 1354.5 cM and 24 linkage groups were detected, corresponding to the haploid chromosome number of the organism. Thirteen to 49 markers for each linkage group were obtained. Conserved synteny between Medaka and zebrafish was observed for 2 independent linkage groups. Unlike zebrafish, however, the Medaka linkage map showed obvious restriction of recombination on the linkage group containing the male-determining region (Y) locus compared to the autosomal chromosomes.
Naruse
K
K
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. naruse@biol.s.u-tokyo.ac.jp
Fukamachi
S
S
Mitani
H
H
Kondo
M
M
Matsuoka
T
T
Kondo
S
S
Hanamura
N
N
Morita
Y
Y
Hasegawa
K
K
Nishigaki
R
R
Shimada
A
A
Wada
H
H
Kusakabe
T
T
Suzuki
N
N
Kinoshita
M
M
Kanamori
A
A
Terado
T
T
Kimura
H
H
Nonaka
M
M
Shima
A
A
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002460:Cell Line; D002874:Chromosome Mapping; D017931:DNA Primers; D005801:Genes, Homeobox; D008040:Genetic Linkage; D016678:Genome; D008285:Major Histocompatibility Complex / Q000235:genetics; D009990:Oryzias / Q000235:genetics*; D012730:Sex Chromosomes
D017931:DNA Primers