[Effect of laminin antisense RNA on the expression of five intracellular proteins stimulated by transforming growth factor beta(1)].
OBJECTIVE
To investigate the effect of laminin antisense RNA on laminin antisense RNA on the expression of E-cadherin, alpha-, beta-, gamma-catenins and focal adhesion kinase(FAK) stimulated by transforming growth factor beta(1)(TGFbeta(1)).


METHODS
Western blot technique was used to study their expression at protein level with ECL reagent.


RESULTS
The expression of alpha- and gamma-catenins was up regulated by TGF beta(1) without the interference of laminin antisense RNA. The up regulation of E-cadherin stimulated by TGFbeta(1) was inhibited in the presence of laminin antisense RNA. No change was found in the expression of FAK when stimulated by TGFbeta(1), alone, but its expression was down regulated with the help of laminin antisense RNA.


CONCLUSIONS
The inhibition of TGFbeta (1) on the malignancy of colon cancer cell may partially be mediated by the up regulation of E-cadherin and catenins. The effect of TGFbeta(1) on the expression of E-cadherin and FAK may be the secondary results of the change in laminin expression.
Liu
B
B
Department of Oncology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi, 710032 P.R. China. brliu@fmmu.edu.cn.
Liu
W
W
Ning
G
G
D015820:Cadherins / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics; D051180:Desmoplakins; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007797:Laminin / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D016372:RNA, Antisense / Q000494:pharmacology*; D015534:Trans-Activators; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D051178:alpha Catenin; D051176:beta Catenin
C495273:CTNNA1 protein, human; C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D007797:Laminin; D016372:RNA, Antisense; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D051178:alpha Catenin; D051176:beta Catenin; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human