title: Selective inhibition of neuronal nitric oxide synthase fails to alter the resting tension and the relaxant effect of bradykinin in isolated rat middle cerebral arteries.
abstract: The role of the neuronal isoform of the nitric oxide (NO) synthase (nNOS) in the regulation of the cerebrovascular tone was studied in vitro. Selective inhibition of nNOS by 7-nitro indazole monosodium salt (7-NINA) failed to alter the resting tension and the relaxant effect of bradykinin in isolated rat middle cerebral arteries. These results indicate that 1./ 7-NINA is selective for nNOS and 2./ cerebrovascular nNOS is involved neither in the resting NO production nor in the mediation of the relaxant effect of bradykinin. Therefore, nNOS-derived NO that contributes to the maintenance of the resting cerebral blood flow in vivo appears to be released from neurons and/or glial cells.
authors.affiliation: Department of Physiology, Ludwig-Maximilians University, Munich, Germany. benyo@elet2.sote.hu
authors.forename: Z
authors.initials: Z
authors.lastname: Benyó
authors.forename: Z
authors.initials: Z
authors.lastname: Lacza
authors.forename: C
authors.initials: C
authors.lastname: Görlach
authors.forename: M
authors.initials: M
authors.lastname: Wahl
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D066298:In Vitro Techniques; D007191:Indazoles / Q000494:pharmacology*; D008297:Male; D020768:Middle Cerebral Artery / Q000187:drug effects* / Q000201:enzymology; D009129:Muscle Tonus / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052248:Nitric Oxide Synthase Type I; D051381:Rats; D017208:Rats, Wistar; D014544:Uridine Triphosphate / Q000494:pharmacology; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D007191:Indazoles; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D001920:Bradykinin; D014544:Uridine Triphosphate; C080122:7-nitroindazole