title: Changes of serum levels of interleukin-2, intercellular adhesion molecule-1, endothelial leukocyte adhesion molecule-1 and Th1 and Th2 cell in severe atopic dermatitis after intravenous immunoglobulin therapy.
abstract: OBJECTIVE
  To evaluate the effect of intravenous immunoglobulin (IVIG) in the treatment of severe intractable atopic dermatitis (AD) in children and to investigate the inflammatory markers used to measure their disease activity.
  
  
  METHODS
  Serum levels of interleukin-2 receptor (sIL-2R), intercellular adhesion molecule-1 (ICAM-1), endothelial leukocyte adhesion molecule (ELAM-1), and eosinophil cationic protein (ECP) were measured in five children with AD (group A) who had a mean age of 9.4 months (range 7 to 12 months) before and after IVIG therapy. Seven age-matched patients with similar severity of AD who only received topical corticosteroid therapy served as the control group (group B). Ten normal control serum samples were collected from well-baby clinic (group C). T helper 1 (Th1) was defined by IFN-gamma/CD4+ and Th2 by IL4/CD4+, using 3-colored flow cytometry. Clinical severity of AD was evaluated with the SCORAD index. Intravenous immunoglobulin (2 g/kg/dose) was administered monthly for a total of 3 doses.
  
  
  RESULTS
  The serum levels of ICAM-1, ELAM-1, and IL-2R in patients with AD were significantly higher than normal control infants. After IVIG therapy, the SCORAD index and the inflammatory markers (ICAM-1, ELAM-1, and ECP) in group A were significantly decreased (P = .01 for SCORAD index; .034, .043, and .03 for ICAM-1, ELAM-1 and ECP, respectively). The serum levels of ICAM-1, ELAM-1, ECP and IL-2R in group B did not show a significant reduction after 3 months of topical corticosteroid therapy. In comparison to normal healthy children, patients with AD had decreased Th2 cells (P = .009) and higher ratio of Th1/Th2 (P = .009) in peripheral blood mononuclear cells (PBMC). There was no significant difference of Th1, Th2 cells, and ratio of Th1/Th2 in PBMC before and after IVIG therapy in patients with AD.
  
  
  CONCLUSIONS
  Intravenous immunoglobulin can be safely and effectively given for the treatment of severe intractable AD. The determination of ICAM-1, ELAM-1, and ECP levels may be useful in monitoring disease activity of AD in childhood. The IVIG may play a role in treatment.
authors.affiliation: Department of Pediatrics, Chang Gung Children's Hospital and Chang Gung University, Taoyuan, Taiwan.
authors.forename: J L
authors.initials: JL
authors.lastname: Huang
authors.forename: W Y
authors.initials: WY
authors.lastname: Lee
authors.forename: L C
authors.initials: LC
authors.lastname: Chen
authors.forename: M L
authors.initials: ML
authors.lastname: Kuo
authors.forename: K H
authors.initials: KH
authors.lastname: Hsieh
mesh_terms: D003876:Dermatitis, Atopic / Q000097:blood* / Q000188:drug therapy / Q000473:pathology*; D019040:E-Selectin / Q000097:blood; D005260:Female; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use*; D007223:Infant; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D007376:Interleukin-2 / Q000097:blood*; D018655:Lymphocyte Count; D008297:Male; D018417:Th1 Cells / Q000473:pathology*; D018418:Th2 Cells / Q000473:pathology*
chemical_list: D019040:E-Selectin; D016756:Immunoglobulins, Intravenous; D007376:Interleukin-2; D018799:Intercellular Adhesion Molecule-1