Controlled oxidation of calf thymus DNA to produce standard samples for 8-oxodeoxyguanosine analysis; effects of freeze-drying, storage and hydrolysis conditions.
Calf thymus DNA containing defined levels of 8-hydroxy-2'-deoxyguanosine (8-oxodG) was prepared by treatment with visible light in the presence of photosensitiser Ro 19-8022. The DNA was checked for stability; after freeze-drying, the amount of 8-oxodG did not increase during 6 weeks' storage at room temperature. However, freeze-drying itself can introduce additional oxidative damage. Two enzymic hydrolysis regimes (DNase I, phosphodiesterases I and II, and alkaline phosphatase; or P1 nuclease and alkaline phosphatase) give similar values for 8-oxodG.
Wood
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DNA Instability Group, Rowett Research Institute, Aberdeen, Scotland, UK.
Gedik
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Collins
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D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004355:Drug Stability; D005612:Freeze Drying; D006868:Hydrolysis; D008027:Light; D010084:Oxidation-Reduction; D017319:Photosensitizing Agents / Q000494:pharmacology; D011759:Pyrrolidines / Q000494:pharmacology; D011807:Quinolizines / Q000494:pharmacology; D013950:Thymus Gland / Q000737:chemistry
D017319:Photosensitizing Agents; D011759:Pyrrolidines; D011807:Quinolizines; C076809:Ro 19-8022; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine