title: Plum pox virus capsid protein mobility in SDS-polyacrylamide gel electrophoresis.
abstract: Slovak plum pox virus (PPV) isolates BOR-3 and KR-4 behaved atypically in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE) of their capsid proteins (CPs). Whereas other tested PPV isolates could be sorted by SDS-PAGE into groups corresponding to the M or D strains, as controlled by restriction fragment length polymorphism (RFLP) analysis, the two above mentioned isolates behaved as the M strain according to RFLP analysis but not SDS-PAGE. Slight mobility differences were observed also among the isolates belonging to the D strain. SDS-PAGE of the CP thus cannot clearly distinguish between these two main PPV strains. BOR-3 isolate has been shown atypical also in its biological properties, and it reacted very weakly with a monoclonal antibody (MAb) recognizing well both M and D strains in immunoblot analysis.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic. virusubr@savba.savba.sk
authors.forename: Z
authors.initials: Z
authors.lastname: Subr
authors.forename: M
authors.initials: M
authors.lastname: Glasa
mesh_terms: D002213:Capsid / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D010935:Plant Diseases / Q000821:virology*; D017801:Plum Pox Virus / Q000145:classification / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D018154:Slovakia; D012967:Sodium Dodecyl Sulfate
chemical_list: D012967:Sodium Dodecyl Sulfate