Influence of the dominant follicle on oocytes from subordinate follicles.
As the oocyte grows within the follicle, a number of factors influence its health and developmental competence. These factors include follicle size, day of estrous cycle, level of atresia and influence of other follicles such as the dominant follicle. Follicles were dissected from ovaries of synchronized dairy cows on four days during the estrous cycle, and the oocyte from each follicle collected, matured, fertilized and cultured singly until Day 8. Development to blastocyst was greater in oocytes collected during phases of follicular growth than those collected during phases of follicular dominance (P<0.001) over all follicle size categories. Oocyte competence tended to increase with increasing follicle size (P<0.1). Follicular cells analyzed by flow cytometry showed an increase in proportion of apoptotic cells in subordinate follicles during the dominant phase compared to growth phase (P<0.05). Thus, the dominant follicle on both oocyte competence and levels of atresia. Further studies on the effect of dominance has shown that lactate production in cumulus-oocyte-complexes (COCs) from medium-sized follicles collected during a dominance phase and small follicles collected during a growth phase are no different from other follicles, despite having significantly lower uptake of glucose (P<0.1). Thus, COCs from different follicle subclasses differ in their nutrient requirements, and current IVM technology needs further improvement to better assist those oocytes that are developmentally challenged.
Hagemann
L J
LJ
AgResearch Ruakura, Hamilton, New Zealand.
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle / Q000502:physiology*; D004971:Estrus / Q000502:physiology; D004973:Estrus Synchronization; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D005947:Glucose / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis; D008297:Male; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D006080:Ovarian Follicle / Q000502:physiology* / Q000648:ultrastructure
D019344:Lactic Acid; D007334:Insulin-Like Growth Factor I; D005947:Glucose