Evaluation of a new commercially available immunoglobulin M and immunoglobulin G immunochromatographic test for diagnosis of melioidosis infection.
An immunochromatographic test for the rapid determination of immunoglobulin M (IgM) and IgG antibodies to Burkholderia pseudomallei was evaluated by using sera from bacteriologically confirmed melioidosis patients and high-risk and clinically suspected patients, along with disease control groups. The sensitivities were 100 and 93% for the IgG and IgM tests, respectively, while the specificity was 95% for both assays. The test was rapid and simple to perform, with results obtained in 10 min.
Cuzzubbo
A J
AJ
PanBio Pty., Ltd., Windsor, Queensland, Australia.
Chenthamarakshan
V
V
Vadivelu
J
J
Puthucheary
S D
SD
Rowland
D
D
Devine
P L
PL
D000907:Antibodies, Bacterial / Q000097:blood*; D016957:Burkholderia pseudomallei / Q000276:immunology*; D002845:Chromatography / Q000379:methods; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008554:Melioidosis / Q000175:diagnosis* / Q000382:microbiology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic