[(35)S]-GTPgammaS autoradiography reveals alpha(2) adrenoceptor-mediated G-protein activation in amygdala and lateral septum.
alpha(2)-adrenoceptor-mediated G-protein activation was examined by [(35)S]-GTPgammaS autoradiography. In alpha(2)-adrenoceptor-rich regions (amygdala, lateral septum), noradrenaline stimulated [(35)S]-GTPgammaS binding. These actions were abolished by the selective alpha(2) antagonist, atipamezole. Conversely, in caudate nucleus, which expresses few alpha(2) receptors, noradrenaline-induced stimulation was not inhibited by atipamezole, suggesting that it is not mediated by alpha(2)-adrenoceptors.
Newman-Tancredi
A
A
Department of Psychopharmacology, Institut de Recherches Servier, 125 Chemin de Ronde, 78290 Croissy-sur-Seine, Paris, France. newman_tancredi@hotmail.com
Chaput
C
C
Touzard
M
M
Millan
M J
MJ
D018674:Adrenergic Antagonists / Q000494:pharmacology; D000679:Amygdala / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D019204:GTP-Binding Proteins / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism*; D007093:Imidazoles / Q000494:pharmacology; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D018341:Receptors, Adrenergic, alpha-2 / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D020665:Septum of Brain / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D018674:Adrenergic Antagonists; D007093:Imidazoles; D018341:Receptors, Adrenergic, alpha-2; D013462:Sulfur Radioisotopes; C050701:atipamezole; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins; D009638:Norepinephrine