Identification of regulatory regions of the putative tumor suppressor gene DMBT1.
The lack of expression of the putative tumor suppressor gene DMBT1 has been described in gliomas, lung carcinomas, and other malignancies. To investigate the mechanisms regulating DMBT1 expression we have screened a human genomic library with a 5' cDNA probe and identified a fragment of approximately 3 kb upstream of the proposed coding sequence of DMBT1. This fragment contains subregions that may up- or down-regulate the expression of a reporter gene. These findings may help to elucidate the mechanisms regulating the alternative splicing and the tissue specific expression of DMBT1.
Lualdi
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E
Unit of Neuro-Oncology and Gene Therapy, Istituto Nazionale Neurologico Besta, via Celoria 11, Milan, 20133, Italy.
Chiariello
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E
Finocchiaro
G
G
D000373:Agglutinins; D001483:Base Sequence; D002135:Calcium-Binding Proteins; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D016147:Genes, Tumor Suppressor; D006801:Humans; D011956:Receptors, Cell Surface / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D025521:Tumor Suppressor Proteins
D000373:Agglutinins; D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D017931:DNA Primers; D004268:DNA-Binding Proteins; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D015500:Chloramphenicol O-Acetyltransferase