title: Mutually exclusive expression of beta(III)-tubulin and vimentin in adrenal cortex carcinoma SW13 cells.
abstract: During embryogenesis, the maturation of neuroblasts into neurones is accompanied by the down-regulation of vimentin and by the expression of neuronal microtubular proteins. Here, we show that human adrenal cortex SW13 cells express beta(III)-tubulin, MAP2b and tau. Analysis of vimentin-positive and -negative subclones of SW13 cells revealed that, under defined cultured conditions, beta(III)-tubulin and MAP2b were present only in vimentin-deficient cells and that beta(III)-tubulin repression occurred at the transcriptional level in vimentin-positive cells. These results suggest that vimentin repression and beta(III)-tubulin expression are co-ordinated by an upstream mechanism relevant to the control of cytoskeletal protein expression during neuronal development.
authors.affiliation: Department of Neurology, Institute of Psychiatry, King's College London, De Crespigny Park, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Butler
authors.forename: J
authors.initials: J
authors.lastname: Robertson
authors.forename: J M
authors.initials: JM
authors.lastname: Gallo
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000306:Adrenal Cortex Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D020868:Gene Silencing; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics; D014404:Tubulin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; C498585:TUBB3 protein, human; D014404:Tubulin; D014746:Vimentin; D016875:tau Proteins