Human IgG1 and IgG3 recognition of Schistosoma mansoni 14kDa fatty acid-binding recombinant protein.
The Schistosoma mansoni gene coding for a 14-kDa fatty acid-binding protein was amplified by PCR and subcloned into the prokaryotic expression vector pMAL-c2. Escherichia coli DH5alpha was transformed with the pMAL-Sm14 construct, and gene expression was induced hr isopropyl-beta-D-thiogalactopyranoside. The resulting recombinant (r) fusion protein was purified by affinity chromatography and confirmed by immunoblot analysis using antimaltose-binding protein or anti-Sm14 antibodies. Additionally, an antibody isotype profile was determined in sera of schistosomiasis patients to rSm14 or soluble adult worm antigen preparation. IgG1 and IgG3 subclass antibodies to rSm14 were predominant in sera of all patients studied whereas low levels of IgM, IgA or IgE were measured. Expression of a S. mansoni gene encoding a vaccine candidate is an important step to better study human immune responses to defined antigens.
Brito
C F
CF
Department of Biochemistry and Immunology, Belo Horizonte-MG, São Paulo, Brazil.
Fonseca
C T
CT
Goes
A M
AM
Azevedo
V
V
Simpson
A J
AJ
Oliveira
S C
SC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology; D003001:Cloning, Molecular; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D008297:Male; D026901:Membrane Transport Proteins; D008875:Middle Aged; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*
D000909:Antibodies, Helminth; D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D015801:Helminth Proteins; D007074:Immunoglobulin G; D026901:Membrane Transport Proteins; D011993:Recombinant Fusion Proteins; C068709:SM14 protein, Schistosoma mansoni