Induction of human cytotoxic T lymphocytes by artificial antigen-presenting cells.
The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were retrovirally transduced with a single HLA-peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any specific peptide.
Latouche
J B
JB
Department of Human Genetics Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Sadelain
M
M
D016475:3T3 Cells; D019264:Adoptive Transfer; D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D018968:CD58 Antigens / Q000235:genetics; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005822:Genetic Vectors; D015789:HLA-A2 Antigen / Q000235:genetics* / Q000276:immunology; D015793:HLA-B7 Antigen / Q000235:genetics; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology; D012190:Retroviridae; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
D018968:CD58 Antigens; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1