title: Evidence for the stimulatory effect of resveratrol on Ca(2+)-activated K+ current in vascular endothelial cells.
abstract: OBJECTIVE
  Resveratrol, a natural phytoalexin compound, is present in grapes and wine, and it can produce vasorelaxation. However, little is known of its mechanisms of action on ionic currents in endothelial cells.
  
  
  METHODS
  The effect of resveratrol on Ca(2+)-activated K+ currents in an endothelial cell line (HUV-EC-C) originally derived from human umbilical vein was investigated with the aid of the patch-clamp technique.
  
  
  RESULTS
  In the whole-cell configuration, resveratrol reversibly increased the amplitude of K+ outward currents. The increase in outward current caused by resveratrol was greatly inhibited by iberiotoxin (200 nM) or paxilline (1 microM), but not by glibenclamide (10 microM), tamoxifen (10 microM), or beta-bungarotoxin (200 nM). Thus, this outward current is believed to be Ca(2+)-activated K+ current (I K(Ca)). In the inside-out configuration, bath application of resveratrol (30 microM) caused no change in the single-channel conductance, but increased the activity of large-conductance Ca(2+)-activated K+ (BKCa) channels. Resveratrol enhanced the channel activity in a concentration-dependent manner. The EC50 value for resveratrol-induced channel activity was 20 microM. The resveratrol-stimulated increase in the channel activity was independent of internal Ca2+. Resveratrol (30 microM) also shifted the activation curve of BKCa channels to less positive membrane potentials. The change in the kinetic behavior of BKCa channels caused by resveratrol in these cells in due to an increase in mean open time and a decrease in mean closed time. In a pancreatic islet endothelial cell line (MS1), resveratrol (30 microM) also increased the activity of intermediate-conductance KCa channels.
  
  
  CONCLUSIONS
  These results provide evidence that in addition to the presence of antioxidative activity, resveratrol can also stimulate KCa channels in endothelial cells. The direct stimulation of these KCa channels by resveratrol may be responsible for its effect on the functional activities of endothelial cells.
authors.affiliation: Department of Medical Education and Research, Veterans General Hospital-Kaohsiung, Taiwan.
authors.forename: H F
authors.initials: HF
authors.lastname: Li
authors.forename: S A
authors.initials: SA
authors.lastname: Chen
authors.forename: S N
authors.initials: SN
authors.lastname: Wu
mesh_terms: D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects*; D007515:Islets of Langerhans / Q000187:drug effects; D018408:Patch-Clamp Techniques; D051419:Paxillin; D010455:Peptides / Q000494:pharmacology; D010750:Phosphoproteins / Q000494:pharmacology; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000187:drug effects*; D013267:Stilbenes; D013268:Stimulation, Chemical; D014471:Umbilical Veins / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D003598:Cytoskeletal Proteins; D005960:Glucosides; C496061:PXN protein, human; D051419:Paxillin; D010455:Peptides; D010750:Phosphoproteins; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D013267:Stilbenes; D014665:Vasodilator Agents; C109945:resveratrol 3-O-glucopyranoside; C064719:iberiotoxin