[Sequence alterations in p53 gene of hepatocellular carcinoma from high aflatoxin risk area in Guangxi].
OBJECTIVE
Check on the sequence alterations of the p53 gene of hepatocellular carcinoma (HCC) in some areas of Guangxi.


METHODS
40 cases of archival HCC samples were divided into two groups. One includes 26 cases from Fusui--an area notorious for high risk in HCC, AFB1 and HBV; The other includes 14 cases from Yulin where both HCC and HBV were high but AFB1 may be low because rice low in AFB1 was the staple food. Exon 7 were sequenced by DNA direct sequencing. p53 mutational protein and HBsAg were examined by immunostaing.


RESULTS
In the Fusui group, 16/26 (61.5%) cases showed mutation at codon 249 third position G to T transversion to form a hot-spot which is generally considered to be caused by AFB1. In the Yulin group, only one single place-Guigang showed p53 mutation clustered at the hot-spot like Fusui in 3/5 cases (60%), while the other cases demonstrated mutational points in various positions.


CONCLUSIONS
Besides Qidong and South Africa, a third AFB1 high risk area was demonstrated where HCC is accompanied by p53 gene specific mutation.
Deng
Z
Z
Guangxi Medical University, Nanning.
Pan
L
L
Ma
Y
Y
D000328:Adult; D016604:Aflatoxin B1; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000235:genetics*; D005260:Female; D005516:Food Microbiology; D016158:Genes, p53; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D003313:Zea mays / Q000382:microbiology
D006514:Hepatitis B Surface Antigens; D016159:Tumor Suppressor Protein p53; D016604:Aflatoxin B1