Cloning of a rat glia maturation factor-gamma (rGMFG) cDNA and expression of its mRNA and protein in rat organs.
We isolated a rat glia maturation factor-gamma(rGMFG) cDNA and examined the tissue distribution of GMFG in rat by Northern and Western blot analyses. Sequence analysis of the entire cDNA revealed an open reading frame of 426 nucleotides with a deduced protein of 142 amino acid residues. The deduced amino acid sequence of the putative product is highly homologous (78.9%) to rat glia maturation factor-beta (rGMFB). Northern blot analysis indicated that a 0.9-kb mRNA is predominantly expressed in rat thymus, testis, and spleen. GMFG showed a different tissue distribution from GMFB, being present predominantly in proliferative and differentiative organs.
Tsuiki
H
H
Department of Pediatrics and Department of Bioregulation Research, Nagoya City University Medical School, Mizuho-ku, Nagoya 467-8601, Japan. tsuiki@med.nagoya-cu.ac.jp
Asai
K
K
Yamamoto
M
M
Fujita
K
K
Inoue
Y
Y
Kawai
Y
Y
Tada
T
T
Moriyama
A
A
Wada
Y
Y
Kato
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D020904:Glia Maturation Factor / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution
D018076:DNA, Complementary; D020904:Glia Maturation Factor; D012333:RNA, Messenger; C117487:glia maturation factor gamma