[Genistein suppresses growth stimulatory effect of growth factors in HCE 16/3 cells].
OBJECTIVE
The role of genistein in the effect of growth factors (KGF and EGF) on the growth of HPV 16 DNA-immortalized human cervical epithelial cells (HCE 16/3 cells) was studied.


METHODS
Genistein affected the modulation effect of the growth factors on the growth of HCE 16/3 cells using [3H]-thymidine incorporation, immunocapture assay of uPA and tyrosine kinase assay.


RESULTS
The growth factors stimulated growth of HCE 16/3 cells in serum-free medium. However, genistein suppressed the growth stimulatory effect of growth factors on HCE 16/3 cells and also reduced KGF-enhanced uPA secretion in this cell line. The inhibitory effects might be associated with its attenuation of activity of tyrosine kinases of growth factors.


CONCLUSIONS
The results suggest that genistein have potential value in the prevention and treatment of some tumors in vivo.
Zheng
J
J
Cancer Institute (Hospital), Chinese Academy of Medical Science, Peking Union Medical College, Beijing.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018914:Cells, Immobilized; D002584:Cervix Uteri / Q000166:cytology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D019833:Genistein / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D000970:Antineoplastic Agents; C496453:FGF7 protein, human; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D019833:Genistein; D014568:Urokinase-Type Plasminogen Activator