title: Cyclin D1 expression is predictive of occult metastases in head and neck cancer patients with clinically negative cervical lymph nodes.
abstract: BACKGROUND
  The aim of this study was to investigate the value of p53 and cyclin D1 gene expression in predicting the risk of occult lymph node metastases in patients with head and neck squamous cell carcinoma (HNSCC).
  
  
  METHODS
  The expression of cyclin D1 and p53 was evaluated by means of immunohistochemical analysis in 32 HNSCC patients with clinically and radiologically negative lymph nodes in whom metastatic involvement was subsequently demonstrated at histologic examination (pN+). A group of 64 head and neck cancer patients with histologically negative laterocervical lymph nodes (pN0) was used as a control.
  
  
  RESULTS
  Cyclin D1 and p53 expression were observed respectively in 42 (43.7%) and 48 cases (50%). Cyclin D1 expression significantly correlated with tumor extension and advanced clinical stage (p =.002 and p =.001, respectively). At univariate regression analysis, cyclin D1 expression significantly correlated with the presence of occult lymph node metastases (p =. 0007), and it remained an independent predictor at multivariate regression analysis (p =.0059).
  
  
  CONCLUSIONS
  Our study indicates that the expression of cyclin D1 correlates with the presence of occult cervical metastases in head and neck carcinoma patients, thus suggesting that its immunohistochemical evaluation in biopsy samples may be used as an additional tool for identifying patients to be treated with elective neck dissection.
authors.affiliation: Dipartimento di Scienze Otorinolaringologiche, Ospedale Maggiore, Istituto di Ricovero e Cura a Carattere Scientifico (I.R.C.C.S.), Via F. Sforza 35, 20122 Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Capaccio
authors.forename: G
authors.initials: G
authors.lastname: Pruneri
authors.forename: N
authors.initials: N
authors.lastname: Carboni
authors.forename: A V
authors.initials: AV
authors.lastname: Pagliari
authors.forename: M
authors.initials: M
authors.lastname: Quatela
authors.forename: B M
authors.initials: BM
authors.lastname: Cesana
authors.forename: L
authors.initials: L
authors.lastname: Pignataro
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis*; D001707:Biopsy, Needle; D002294:Carcinoma, Squamous Cell / Q000401:mortality / Q000473:pathology* / Q000556:secondary*; D019938:Cyclin D1 / Q000032:analysis*; D005260:Female; D006258:Head and Neck Neoplasms / Q000401:mortality / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1