title: Interaction of bull, stallion and boar seminal plasma proteins and sperms with acidic polysaccharides.
abstract: The interaction of seminal plasma proteins, sperms and detergent-released sperm proteins of three species with different types of acidic polysaccharides was studied. Heparin-binding activity of boar, bull and stallion seminal plasma proteins, sperms and sperm proteins was compared with their ability to interact with polysaccharides differing in the presence of the sulfate groups or in their saccharide moiety (chondroitin sulfate, dextran sulfate, fucoidan, hyaluronic acid). Bull seminal plasma proteins were characterized by higher affinity to heparin, fucoidan and dextran sulfate, while significant differences between different types of polysaccharides were detected in the case of boar proteins. Sperm protein interactions with acidic polysaccharides in bull and stallion were analogous to the binding of seminal plasma proteins. Bull and stallion seminal plasma proteins inhibited the interaction of corresponding sperm proteins with acidic polysaccharides.
authors.affiliation: Department of Biochemistry, Faculty of Natural Science, Charles University, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Liberda
authors.forename: M
authors.initials: M
authors.lastname: Tichá
authors.forename: Z
authors.initials: Z
authors.lastname: Zralý
authors.forename: D
authors.initials: D
authors.lastname: Svecová
authors.forename: Z
authors.initials: Z
authors.lastname: Vezník
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D006736:Horses; D066298:In Vitro Techniques; D008297:Male; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012661:Semen / Q000378:metabolism*; D013045:Species Specificity; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine
chemical_list: D011134:Polysaccharides; D011506:Proteins; D006493:Heparin