Immunostimulatory activity of the bacterial extract OM-8.
The bacterial extract OM-89 used for the prevention and treatment of recurrent urinary tract infections constitutes an effective immunostimulant in vitro and in vivo. Here we demonstrate that OM-89 shows mitogenic properties towards murine spleen cell cultures from LPS responder and non-responder mice. In macrophages the extract induces the translocation of NF-kappaB into the cell nucleus and RNI (radical nitrogen intermediates) release, which could be attributed to single fractions of the extract. Our findings on the in vitro immunostimulatory effect of OM-89, as well as its immunogenic and adjuvant properties, are of importance for understanding its therapeutic efficacy as demonstrated in clinical studies.
Huber
M
M
Institut für Molekulare Medizin und Zellforschung, AG Tumorimmunologie/Vakzine, Medizinische Fakultät der Universität, Stefan-Meier-Str. 8, D-79104 Freiburg, Germany.
Ayoub
M
M
Pfannes
S D
SD
Mittenbühler
K
K
Weis
K
K
Bessler
W G
WG
Baier
W
W
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology; D002460:Cell Line; D002478:Cells, Cultured; D004926:Escherichia coli; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000502:physiology; D011994:Recombinant Proteins; D012475:Salmonella; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011994:Recombinant Proteins; C050501:OM 89; D009569:Nitric Oxide; D007371:Interferon-gamma