Mutation in bombesin receptor subtype-3 gene is not a major cause of obesity in the Japanese.
Bombesin receptor subtype-3 (BRS-3) is one of the candidate genes of obesity. The mice lacking BRS-3 have been shown to develop mild obesity. These mice also showed hypertension and impaired glucose metabolism, supporting these mice as a good model for human obesity. We screened 104 Japanese obese men (BMI > 26.4, 26.5-44.1) to investigate whether there is any genetic defect in BRS-3 gene. The DNA fragments containing each exon of BRS-3 gene were amplified by polymerase chain reaction (PCR) and were directly sequenced. No mutation, nor polymorphism was found in the coding region of BRS-3, suggesting that mutation of this gene is not a major cause of obesity in humans.
Hotta
K
K
Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Suita, Japan. khotta@imed2.med.osaka-u.ac.jp
Matsukawa
Y
Y
Nishida
M
M
Kotani
K
K
Takahashi
M
M
Kuriyama
H
H
Nakamura
T
T
Wada
K
K
Yamashita
S
S
Funahashi
T
T
Matsuzawa
Y
Y
D017931:DNA Primers; D005820:Genetic Testing; D006801:Humans; D007564:Japan; D009154:Mutation; D009765:Obesity / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018004:Receptors, Bombesin / Q000235:genetics*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018004:Receptors, Bombesin; C107120:bombesin receptor subtype 3