Prostaglandin F(2alpha) (PGF(2alpha)) induces cyclin D1 expression and DNA synthesis via early signaling mechanisms in Swiss mouse 3T3 cells.
Prostaglandin F(2alpha) (PGF(2alpha)), a mitogen for Swiss 3T3 cells, triggers cyclin D1 mRNA/protein expression prior to cellular entry into the S phase, but fails to raise cdk4 or cyclin D3 levels, while 1-oleoyl-2-diacylglycerol (OAG), a protein kinase C (PKC) and tyrosine kinase (TK) activator, induces only cyclin D1 expression with no mitogenic response. In contrast, in PKC-depleted or -inhibited cells, PGF(2alpha), but not OAG, increases cyclin D1 expression with no mitogenic response. Finally, OAG, in the presence of orthovanadate (Na(3)VO(4)) or TGF(beta1), induces DNA synthesis. Thus, it appears that PGF(2alpha) triggers cyclin D1 expression via two independent signaling events that complement with TGF(beta1)-triggered events to induce DNA synthesis.
Sauane
M
M
Instituto de Investigaciones Bioquímicas "Luis F. Leloir" Fundación Campomar, Avenida Patricias Argentinas 435, Buenos Aires, 1405, Argentina.
Correa
L
L
Rogers
F
F
Krasnapolski
M
M
Barraclough
R
R
Rudland
P S
PS
de Asúa
L J
LJ
D016475:3T3 Cells; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D019938:Cyclin D1 / Q000096:biosynthesis* / Q000235:genetics; D004261:DNA Replication / Q000187:drug effects; D015237:Dinoprost / Q000494:pharmacology*; D005786:Gene Expression Regulation; D005989:Glycerides / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D008954:Models, Biological; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
D005989:Glycerides; D007476:Ionophores; D008934:Mitogens; D016212:Transforming Growth Factor beta; D019938:Cyclin D1; D014638:Vanadates; D015237:Dinoprost; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C