title: Dual-viral vector approach induced strong and long-lasting protective immunity against very virulent infectious bursal disease virus.
abstract: To induce strong protective immunity against very virulent infectious bursal disease virus (vvIBDV) in chickens, two viral vector systems, Marek's disease and Fowlpox viruses expressing the vvIBDV host-protective antigen VP2 (rMDV, rFPV), were used. Most of chickens vaccinated with the rFPV or rMDV alone, or vaccinated simultaneously with both at their hatch (rMDV-rFPV(1d)), were protected against developing clinical signs and mortality; however, only zero to 14% of the chickens were protected against gross lesions. In contrast, gross lesions were protected in 67% of chickens vaccinated primarily with the rMDV followed by boosting with the rFPV 2 weeks later (rMDV-rFPV(14d)). Protection against the severe histopathological lesions of rFPV, rMDV, rMDV-rFPV(1d), and rMDV-rFPV(14d) vaccine groups were 33, 42, 53, and 73%, respectively. Geometric mean antibody titers to VP2 of chickens vaccinated with the rFPV, rMDV, rMDV-rFPV(1d), and rMDV-rFPV(14d) before the challenge were 110, 202, 254, and 611, respectively. Persistent infection of the rMDV in chickens after the booster vaccination with rFPV was suggested by detection of the rMDV genes from peripheral blood lymphocyte DNA at 28 weeks of age. These results indicate that the dual-viral vector approach is useful for quickly and safely inducing strong and long-lasting protective immunity against vvIBDV in chickens.
authors.affiliation: Department of Virology, National Institute of Animal Health, Tsukuba, Ibaraki, 305-0856, Japan. ktsukamo@niah.affrc.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Tanimura
authors.forename: N
authors.initials: N
authors.lastname: Hamazaki
authors.forename: M
authors.initials: M
authors.lastname: Mase
authors.forename: S
authors.initials: S
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control*; D002645:Chickens; D005587:Fowlpox virus; D005822:Genetic Vectors; D008381:Herpesvirus 2, Gallid; D007243:Infectious bursal disease virus / Q000276:immunology; D011201:Poultry Diseases / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary; D015678:Viral Structural Proteins / Q000008:administration & dosage / Q000276:immunology*
chemical_list: C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins