title: Immune response with biodegradable nanospheres and alum: studies in rabbits using staphylococcal enterotoxin B-toxoid.
abstract: In this study, the adjuvant effect of the sustained release biodegradable nanospheres (100-150 nm in diameter) has been compared with alum. Nanospheres were formulated using a biodegradable polylactic polyglycolic acid copolymer (PLGA, 50:50) containing Staphylococcal Enterotoxin B (SEB) toxoid as a model vaccine antigen. Systemic immune response of the nanospheres containing toxoid was studied in rabbits by subcutaneous immunization. The data demonstrated that approximately 30% of the toxoid activity was lost following its encapsulation into nanospheres. Under in vitro conditions, nanospheres demonstrated sustained release of the toxoid. However, only 20% of the antigenic toxoid was released over the first 2 weeks of the release study. Immunization of animals with equal doses of toxoid, either using nanospheres or alum induced a comparable systemic immune response (IgG, IgM and IgA). The immune response reached a maximum level at 7 weeks post-immunization, which then gradually declined with time. The booster dose of toxoid at 19 weeks, either using alum or nanospheres induced similar immune response in both the groups, but was greater than the primary immune response. The studies, thus, suggest that biodegradable nanospheres could be used as a vaccine adjuvant.
authors.affiliation: University of Michigan, Ann Arbor 48109, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Desai
authors.forename: J M
authors.initials: JM
authors.lastname: Hilfinger
authors.forename: G L
authors.initials: GL
authors.lastname: Amidon
authors.forename: R J
authors.initials: RJ
authors.lastname: Levy
authors.forename: V
authors.initials: V
authors.lastname: Labhasetwar
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000534:Alum Compounds / Q000008:administration & dosage / Q000737:chemistry*; D000818:Animals; D001673:Biodegradation, Environmental; D004306:Dose-Response Relationship, Immunologic; D004337:Drug Carriers; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007279:Injections, Subcutaneous; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D011817:Rabbits; D013208:Staphylococcal Toxoid / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D004337:Drug Carriers; D004768:Enterotoxins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013208:Staphylococcal Toxoid; C041524:aluminum sulfate; C031818:enterotoxin B, staphylococcal