Development of a serological test for Haemophilus ducreyi for seroprevalence studies.
We developed a new enzyme immunoassay (rpEIA) for use in determining the seroprevalence of chancroid. Three highly conserved outer membrane proteins from Haemophilus ducreyi strain 35000 were cloned, overexpressed, and purified from Escherichia coli for use as antigens in the rpEIA. Serum specimens from patients with and without chancroid were assayed to determine optimum sensitivity and specificity and to establish cutoff values. On the basis of these data, rpEIA was found to be both sensitive and specific when used to test a variety of serum specimens from patients with genital ulcers and urethritis and from healthy blood donors.
Elkins
C
C
Departments of Medicine, School of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA.
Yi
K
K
Olsen
B
B
Thomas
C
C
Thomas
K
K
Morse
S
S
D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002602:Chancroid / Q000453:epidemiology* / Q000382:microbiology; D003001:Cloning, Molecular; D006191:Haemophilus ducreyi / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins