Serum and lung levels of thiamphenicol after administration of its glycinate N-acetylcysteinate ester in experimentally infected guinea pigs.
Thiamphenicol is an analogue of chloramphenicol and is characterised by a broad spectrum of action. In this study, serum and lung levels of thiamphenicol (TAP) were studied in infected guinea pigs after the administration of thiamphenicol glycinate N-acetylcysteinate (TGA). Animals received a single dose of TGA (15 mg/kg, subcutaneously) immediately after intra-tracheal infection with Haemophilus influenzae (about 10(7) CFU/animal). Serum and lung concentrations of TAP were determined at 0, 1, 3, 6, 12 and 24 h after drug administration by means of HPLC. TAP serum levels were elevated at 1 h and remained detectable for 24 h after drug administration. Tissue lung levels were comparable to peak serum concentrations but remained higher and decreased more slowly than serum concentrations.
Drago
L
L
Department of Preclinical Science, L.I.T.A. Vialba, L. Sacco Teaching Hospital, University of Milan, Italy. microbio@mailserver.csi.unimi.it
De Vecchi
E
E
Fassina
M C
MC
Mombelli
B
B
Gismondo
M R
MR
D000111:Acetylcysteine / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D015169:Colony Count, Microbial; D004338:Drug Combinations; D005260:Female; D006168:Guinea Pigs; D006192:Haemophilus Infections / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D006193:Haemophilus influenzae; D007279:Injections, Subcutaneous; D008168:Lung / Q000378:metabolism* / Q000382:microbiology; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics
D000900:Anti-Bacterial Agents; D004338:Drug Combinations; C028814:thiamphenicol glycinate acetylcysteinate; D013839:Thiamphenicol; D000111:Acetylcysteine