title: Positioning of the mitotic spindle by a cortical-microtubule capture mechanism.
abstract: Correct positioning of the mitotic spindle is critical for cell division and development. Spindle positioning involves a search-and-capture mechanism whereby dynamic microtubules find and then interact with specific sites on the submembrane cortex. Genetic, biochemical, and imaging experiments suggest a mechanism for cortical-microtubule capture. Bim1p, located at microtubule distal ends, bound Kar9p, a protein associated with the daughter cell cortex. Bim1p is the yeast ortholog of human EB1, a binding partner for the adenomatous polyposis coli tumor suppressor. EB1 family proteins may have a general role in linking the microtubule cytoskeleton to cortical polarity determinants.
authors.affiliation: Departments of Pediatric Oncology, The Dana-Farber Cancer Institute, and Pediatric Hematology, The Children's Hospital, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
authors.forename: L
authors.initials: L
authors.lastname: Lee
authors.forename: J S
authors.initials: JS
authors.lastname: Tirnauer
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: S C
authors.initials: SC
authors.lastname: Schuyler
authors.forename: J Y
authors.initials: JY
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Pellman
mesh_terms: D025601:Adenomatous Polyposis Coli Protein; D001665:Binding Sites; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D016193:G1 Phase; D008868:Microtubule Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D008941:Spindle Apparatus / Q000502:physiology*
chemical_list: D025601:Adenomatous Polyposis Coli Protein; C119683:BIM1 protein, S cerevisiae; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C405804:EB1 microtubule binding proteins; C110443:KAR9 protein, S cerevisiae; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins