DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines.
We investigated the relationship between DNA-dependent protein kinase (DNA-PK) activity and radiation sensitivity using 14 esophageal cancer cell lines, TE 1-14. DNA-PK activities differed significantly among the cell lines. The highest DNA-PK activity observed in TE-8 was more than two times higher than the lowest DNA-PK activity observed in TE-5. Significant correlation was observed between DNA-PK activity and D0 (r = 0.766; P = 0.0008). Western blots analysis revealed a significant correlation between DNA-PK activity and Ku70 expression, suggesting that the regulation in DNA-PK activity was associated with Ku70 expression. The data suggest that the measurement of DNA-PK activity and/or Ku70 expression may provide a useful way to predict radiation sensitivity.
Zhao
H J
HJ
Department of Radiation Research, Tohoku University School of Medicine, Sendai, Japan.
Hosoi
Y
Y
Miyachi
H
H
Ishii
K
K
Yoshida
M
M
Nemoto
K
K
Takai
Y
Y
Yamada
S
S
Suzuki
N
N
Ono
T
T
D034961:Antigens, Nuclear; D015153:Blotting, Western; D002455:Cell Division; D002457:Cell Extracts / Q000737:chemistry; D004265:DNA Helicases; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D003627:Data Interpretation, Statistical; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D000072200:Ku Autoantigen; D009687:Nuclear Proteins / Q000096:biosynthesis*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects
D034961:Antigens, Nuclear; D002457:Cell Extracts; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen