The molecular structure of the fastest myosin from green algae, Chara.
Chara myosin in green algae, Chara corallina, is the fastest myosin of all those observed so far. To shed light on the molecular mechanism of this fast sliding, we determined the primary structure of Chara myosin heavy chain (hc). It has a motor domain, six IQ motifs for calmodulin binding, a coiled-coil structure to dimerize, and a globular tail. Chara myosin hc is very similar to some plant myosins and has been predicted to belong to the class XI. Short loop 1 and loop 2 may account for the characteristics of mechanochemical properties of Chara myosin.
Morimatsu
M
M
Department of Molecular Biology, School of Science, Nagoya University, Chikusaku, Nagoya, 464-8602, Japan.
Nakamura
A
A
Sumiyoshi
H
H
Sakaba
N
N
Taniguchi
H
H
Kohama
K
K
Higashi-Fujime
S
S
D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D000460:Chlorophyta / Q000737:chemistry* / Q000648:ultrastructure; D003595:Cytoplasmic Streaming; D005455:Fluorescent Antibody Technique; D015723:Gene Library; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains / Q000737:chemistry* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000737:chemistry; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D020409:Molecular Motor Proteins; D010446:Peptide Fragments; D018995:Myosin Heavy Chains