Changes in lipoprotein(a) levels measured by six kit methods during growth hormone treatment of growth hormone-deficient adults.
Lipoprotein(a) [Lp(a)], an independent risk factor for cardiovascular disease, has previously been reported to increase, decrease or show no change in growth hormone (GH)-deficient individuals receiving GH replacement. To assess whether these inconsistencies could be attributed to differences in immunoassay methods, Lp(a) was measured by six commercial kits at 0, 3, 6 and 9 months in nine GH-deficient individuals (median age 68.3 years, six male) during 9 months GH therapy. There was a significant rise in Lp(a) with the INCStar immunoturbidimetric (IT) method and the Mercodia enzyme linked immunosorbent assay (ELISA) (P</=0.05, two-tailed Wilcoxon signed rank test), a non-significant rise with the Pharmacia immuno-radiometric assay and the Biopool ELISA methods (P =0.06), and no change with the Immuno ELISA and WAKO IT kits. There was also considerable variation in the values reported within each individual. These results suggest that the previously reported inconsistencies may in part be due to methodological differences, and that the effect of GH on Lp(a) remains unknown. This study highlights the need for a more common approach to the standardization of Lp(a) methods and the selection of antibodies used in them. Better performing methods may allow a more reliable interpretation of the effects of GH on Lp(a)
Wieringa
G
G
Departments of Biochemistry, Endocrinology, Medical Statistics, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, UK.
Toogood
A A
AA
Ryder
W D
WD
Anderson
J M
JM
Mackness
M
M
Shalet
S M
SM
D000368:Aged; D001774:Blood Chemical Analysis / Q000379:methods*; D005260:Female; D019382:Human Growth Hormone / Q000097:blood* / Q000172:deficiency* / Q000627:therapeutic use*; D006801:Humans; D017270:Lipoprotein(a) / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D013997:Time Factors
D017270:Lipoprotein(a); D019382:Human Growth Hormone