title: A flavonoid sulfate antigen activates human alphabeta CD8+ Th2 lymphocytes in pollen allergy.
abstract: Cellular immune responses are initiated when T lymphocytes expressing alphabeta TCR recognize peptide antigens bound to MHC molecules or, less frequently, double-stranded glycolipid antigens bound to CD1 molecules. In the allergy to Parietaria judaica, human alphabeta CD8+ Th2 lymphocytes react to a non-peptide pollinic antigen presented by B cells. The environmental allergen was purified and identified as a new flavonoid pigment: 2'-O-sulfate, 6-O-betaD-glucuronopyranosyl, 2',5,6-trihydroxy-isoflavone. Its specific recognition by alphabeta CD8+ Th2 T cells (1) depends upon an MHC- and CD1-independent presentation mediated by B cells, (2) is determined by the flavonoid carbohydrate and sulfate groups and (3) leads to positive skin prick test in allergic patients. Hence, an unusual mode of aromatic sulfated antigen recognition by alphabeta CD8+ Th2 T lymphocytes might underlie the cellular mediation of human allergy to plant allergens.
authors.affiliation: Basel Institute for Immunology, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Sallusto
authors.forename: R
authors.initials: R
authors.lastname: Poupot
authors.forename: M
authors.initials: M
authors.lastname: Clergue
authors.forename: R
authors.initials: R
authors.lastname: DePalma
authors.forename: J J
authors.initials: JJ
authors.lastname: Fournié
mesh_terms: D000485:Allergens / Q000737:chemistry; D017951:Antigen Presentation; D000941:Antigens / Q000737:chemistry*; D016827:CD8 Antigens / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007529:Isoflavones / Q000737:chemistry* / Q000276:immunology*; D008213:Lymphocyte Activation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D015394:Molecular Structure; D011058:Pollen / Q000737:chemistry / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D019685:Rosales / Q000737:chemistry* / Q000276:immunology*; D012882:Skin Tests; D018418:Th2 Cells / Q000276:immunology*
chemical_list: C406382:6-O-glucuronopyranosyl-2',5,6-trihydroxyisoflavone, 2'-O-sulfate; D000485:Allergens; D000941:Antigens; D016827:CD8 Antigens; D005960:Glucosides; D007529:Isoflavones; D016693:Receptors, Antigen, T-Cell, alpha-beta