title: Biosynthesis and secretion of several enzymes in Escherichia coli dnaK and dnaJ mutants.
abstract: Escherichia coli null dnaJ and dnaKdnaJ mutants were defective in the biosynthesis and secretion of several enzymes. The synthesis of beta-galactosidase induced in delta dnaJ and delta dnaKdnaJ mutants was abolished at 42 degrees C and significantly decreased at 30 and 37 degrees C. The activity of alkaline phosphatase in the periplasm in both mutant strains at high temperature was lower than in the wild-type strain. The synthesis of b-type cytochromes was defective in two deletion mutants while the synthesis of nitrate reductase-A at 42 degrees C was influenced by dnaK mutation only. The lack of DnaK and DnaJ does not impair the activity of catechol 2,3-dioxygenase irrespective of growth temperature.
authors.affiliation: Department of Bacterial Genetics, Warsaw University, Poland.
authors.forename: K I
authors.initials: KI
authors.lastname: Wolska
authors.forename: B
authors.initials: B
authors.lastname: Lobacz
authors.forename: D
authors.initials: D
authors.lastname: Jurkiewicz
authors.forename: E
authors.initials: E
authors.lastname: Bugajska
authors.forename: M
authors.initials: M
authors.lastname: Kuć
authors.forename: A
authors.initials: A
authors.lastname: Jóźwik
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism; D050557:Catechol 2,3-Dioxygenase; D003573:Cytochrome b Group / Q000096:biosynthesis / Q000378:metabolism; D049308:Dioxygenases; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006361:Heating; D018832:Molecular Chaperones / Q000235:genetics*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000096:biosynthesis / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase; C063842:dnaK protein, E coli