An efficient, reliable and inexpensive device for the rapid homogenization of multiple tissue samples by centrifugation.
Homogenization of tissue samples is a common first step in the majority of current protocols for RNA, DNA, and protein isolation. This report describes a simple device for centrifugation-mediated homogenization of tissue samples. The method presented is applicable to RNA, DNA, and protein isolation, and we show examples where high quality total cell RNA, DNA, and protein were obtained from brain and other tissue samples. The advantages of the approach presented include: (1) a significant reduction in time investment relative to hand-driven or individual motorized-driven pestle homogenization; (2) easy construction of the device from inexpensive parts available in any laboratory; (3) high replicability in the processing; and (4) the capacity for the parallel processing of multiple tissue samples, thus allowing higher efficiency, reliability, and standardization.
Ilyin
S E
SE
Molecular Biology, School of Life and Health Sciences, University of Delaware, Newark 19716-2590, USA. 78742@udel.edu
Plata-Salamán
C R
CR
D000818:Animals; D001923:Brain Chemistry; D002498:Centrifugation; D004247:DNA / Q000302:isolation & purification; D008967:Molecular Biology / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D051381:Rats
D009419:Nerve Tissue Proteins; D012313:RNA; D004247:DNA