title: Experimental infection of nonenveloped DNA virus (TTV) in rhesus monkey.
abstract: Virus fragments homologous to TTV were detected previously from an enterically transmitted outbreak of non-A-E hepatitis [Luo et al., 1999]. To test the susceptibility of the Rhesus monkey to this virus and to establish its transmission routes, 6 Rhesus monkeys were inoculated, 3 orally and another 3 intravenously. The inoculum was prepared by extracting and filtering feces collected from a patient during the incubation period identified in the described outbreak. A second group of 3 monkeys was used for the passage study. The feces and blood samples were collected for detection of the virus by polymerase chain reaction (PCR). Four animals were subjected to liver biopsies and bile aspiration by open surgery for in situ virus detection. Viremia occurred in 4-7 days after intravenous and 7-10 days after oral inoculation. The virus was excreted in feces a few days after oral infection and simultaneously with viremia after intravenous inoculation. The virus was also detected in bile during the viremic phase. There was a prolonged carrier state with persistent viremia and virus excretion in feces for more than 6 months. Serum transaminase levels were not raised during the infection. The virus was present in both the cytoplasm and nuclei of hepatocytes, but no significant pathology was found. Therefore, the Rhesus monkey is susceptible to TT virus infection, but the virus seems nonpathogenic. Infection of the liver may be established either by oral or parenteral inoculation. The virus may be released from liver into the blood or via bile into feces, so it may be transmitted by both blood and fecal routes.
authors.affiliation: Department of Infectious Diseases, Nanfang Hospital, Guangzhou, China.
authors.forename: K
authors.initials: K
authors.lastname: Luo
authors.forename: W
authors.initials: W
authors.lastname: Liang
authors.forename: H
authors.initials: H
authors.lastname: He
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Xiao
authors.forename: D
authors.initials: D
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004266:DNA Virus Infections / Q000635:transmission* / Q000821:virology; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D005243:Feces / Q000821:virology; D006524:Hepatitis, Viral, Animal / Q000635:transmission* / Q000821:virology; D006525:Hepatitis, Viral, Human / Q000635:transmission* / Q000821:virology; D006801:Humans; D008099:Liver / Q000648:ultrastructure / Q000821:virology; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004279:DNA, Viral; D000410:Alanine Transaminase