SYT-SSX fusion proteins in synovial sarcomas: detection and characterization with new antibodies.
To identify and characterize the SYT-SSX fusion proteins in synovial sarcomas, we developed two polyclonal antibodies against the N-terminal part and for the C-terminal part of the SYT-SSX2 protein. Specificity was demonstrated on COS-7 cells transfected with two subtypes of SYT-SSX fusion genes, SYT-SSX1 and SYT-SSX2. Both antibodies recognized a single protein of 61 kDa in an immunoprecipitation of the transfected COS-7 cell lysates. These antibodies also detected the native protein of 61 kDa in the lysate of a human synovial sarcoma cell line (HS-SY41) with immunoprecipitation, and in extracts of human synovial sarcomas with western blot analysis. An immunohistochemical study, using human synovial sarcoma tissues, demonstrated that the SYT-SSX fusion proteins localized in the nucleus of the tumor cells. These antibodies provide a useful method for studying the expression of the SYT-SSX fusion proteins.
Hashimoto
N
N
Department of Orthopedic Surgery, Osaka University Medical School, Suita, Japan.
Araki
N
N
Yoshikawa
H
H
Myoui
A
A
Matsumine
A
A
Kaneko
M
M
Sonobe
H
H
Ochi
T
T
D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D013584:Sarcoma, Synovial / Q000276:immunology / Q000378:metabolism*
D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D015514:Oncogene Proteins, Fusion; C116512:SYT-SSX fusion protein