title: Survival and growth of Burkholderia cepacia within the free-living amoeba Acanthamoeba polyphaga.
abstract: The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia.
authors.affiliation: Department of Microbiology, University of Leeds, UK.
authors.forename: P
authors.initials: P
authors.lastname: Landers
authors.forename: K G
authors.initials: KG
authors.lastname: Kerr
authors.forename: T J
authors.initials: TJ
authors.lastname: Rowbotham
authors.forename: J L
authors.initials: JL
authors.lastname: Tipper
authors.forename: P M
authors.initials: PM
authors.lastname: Keig
authors.forename: E
authors.initials: E
authors.lastname: Ingham
authors.forename: M
authors.initials: M
authors.lastname: Denton
mesh_terms: D000048:Acanthamoeba / Q000254:growth & development / Q000382:microbiology*; D000818:Animals; D016956:Burkholderia cepacia / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D006801:Humans
chemical_list: D003470:Culture Media