A modified staining method for connective tissue in semi-thin histological sections of peripheral and cranial nerves.
A simple staining method for collagen in semi-thin sections of nerves is described. This technique consists of applying basic fuchsin (0.05%) solution for 2 min. to +/- 1.25 microns plastic resin-embedded sections after routine toluidine blue staining. This staining method clearly demonstrates the amount and orientation of collagenous connective tissue in the nerve, both in transverse and longitudinal sections.
Menovsky
T
T
Department of Neurosurgery, University Hospital Nijmegen St. Radboud, The Netherlands.
van den Bergh Weerman
M
M
D000818:Animals; D003094:Collagen / Q000648:ultrastructure*; D004396:Coloring Agents; D003238:Connective Tissue / Q000033:anatomy & histology; D003391:Cranial Nerves / Q000033:anatomy & histology*; D008867:Microtomy; D010525:Peripheral Nerves / Q000033:anatomy & histology*; D016613:Plastic Embedding; D051381:Rats; D012394:Rosaniline Dyes; D014048:Tolonium Chloride
D004396:Coloring Agents; D012394:Rosaniline Dyes; D014048:Tolonium Chloride; C025485:basic fuchsin; D003094:Collagen