title: [Preparative dephosphorylation of calcium salts of 2'(3)-mononucleotides by extracellular phosphatase from Spicaria violacea].
abstract: The properties of the phosphatase present in the culture liquid of Spicaria violacea were investigated. Based on these results, a method for preparative dephosphorylation of calcium salts of 2'(3')-mononucleotides was proposed. A 96-98% yield was achieved at a substrate concentration of 100 mg/ml. Mild quantitative hydrolysis of RNA to nucleosides can be performed by RNA digestion to mononucleotides with Ca2+ followed by the proposed dephosphorylation procedure.
authors.affiliation: Institute of Microbiology, National Academy of Sciences of Belarus.
authors.forename: V N
authors.initials: VN
authors.lastname: Baraĭ
authors.forename: I A
authors.initials: IA
authors.lastname: Severina
authors.forename: T A
authors.initials: TA
authors.lastname: Kukharskaia
authors.forename: A I
authors.initials: AI
authors.lastname: Zinchenko
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D005656:Fungal Proteins; D000249:Adenosine Monophosphate; D010744:Phosphoric Monoester Hydrolases