title: New mechanistic insights from structural studies of the oxygen-sensing domain of Bradyrhizobium japonicum FixL.
abstract: The FixL heme domain serves as the dioxygen switch in the FixL/FixJ two-component system of Rhizobia. Recent structural studies of the Bradyrhizobium japonicum FixL heme domain (BjFixLH) have suggested an allosteric mechanism that is distinct from the classical hemoglobin model. To gain further insight into the FixL sensing mechanism, structures of BjFixLH bound to dioxygen, imidazole, and nitric oxide have been determined. These structures, particularly the structure of BjFixLH bound to its physiological ligand, dioxygen, have helped to address a number of important issues relevant to the BjFixLH sensing mechanism. On the basis of the oxy-BjFixLH structure, a conserved arginine is found to stabilize the dioxygen ligand in a mode reminiscent of the distal histidine in classical myoglobins and hemoglobins. The structure of BjFixLH bound to imidazole elucidates the structural requirements for accommodating sterically bulky ligands. Finally, the structure of BjFixLH bound to nitric oxide provides evidence for a structural intermediate in the heme-driven conformational change.
authors.affiliation: Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio 43210, USA.
authors.forename: W
authors.initials: W
authors.lastname: Gong
authors.forename: B
authors.initials: B
authors.lastname: Hao
authors.forename: M K
authors.initials: MK
authors.lastname: Chan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D020369:Bradyrhizobium / Q000737:chemistry* / Q000378:metabolism; D006224:Cricetinae; D006420:Hemeproteins / Q000737:chemistry* / Q000378:metabolism; D000071677:Histidine Kinase; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D011487:Protein Conformation; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006420:Hemeproteins; D011494:Protein Kinases; C067929:FixL protein, Bacteria; D000071677:Histidine Kinase; D010100:Oxygen