Postmortem changes in cytochrome c oxidase activity in various organs of the rat and in human heart.
Cytochrome c oxidase (COX), a mitochondrial enzyme, is inactivated by cyanide or carbon monoxide (CO) intoxication. To test whether cytochrome c has potential as an indicator of these toxins in cadavers, we measured COX activity in the main organs of the rat, and in the human heart, at various times after death. Each tissue sample or organ was homogenized and the COX activity in the mitochondrial fraction was measured using ferrous cytochrome c as the substrate. COX activity was significantly higher in rat brain, heart and kidney than in lung and liver from 0 to 4 days after death. The loss of COX activity was significantly slower in the brain and heart than in the lung, liver and kidney. Most importantly, COX activity correlated with the time-since-death for each of the rat organs we tested (r2=0.70-0.95), but for the human heart (r2=0.47). It may be possible that COX activity is likely to be a useful indicator of the time-since-death, and is worth pursuing as an indicator of the tissue cyanide and CO content.
Ikegaya
H
H
Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, Japan. ikegaya@m.u-tokyo.ac.jp
Iwase
H
H
Hatanaka
K
K
Sakurada
K
K
Matsuda
Y
Y
Kobayashi
M
M
Takatori
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000201:enzymology; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D003576:Electron Transport Complex IV / Q000032:analysis* / Q000037:antagonists & inhibitors; D005554:Forensic Medicine; D006801:Humans; D007223:Infant; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D011180:Postmortem Changes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D013997:Time Factors; D014018:Tissue Distribution
D015415:Biomarkers; D003576:Electron Transport Complex IV