Inhibition of early DNA-damage and chromosomal aberrations by Trianthema portulacastruml. In carbon tetrachloride-induced mouse liver damage.
The underlying molecular mechanisms of the antihepatotoxic activity of Trianthema portulacastrum by monitoring its effect on mouse liver DNA-chain break, sugar-base damage and chromosomal aberrations, during chronic or acute treatment with carbon tetrachloride (CCl(4)) have been studied. Daily oral feeding with the ethanolic extract (150 mg/kg basal diet, per os) was given 2 weeks before CCl(4)treatment and continued until the end of the experiment (13 weeks). T. portulacastrum extract offer unique protection (P< 0.05-0. 001) against the induction of liver-specific structural-type chromosomal anomalies 15, 30 or 45 days after the last CCl(4)insult, compared to control mice. This was further evidenced by extract-mediated protection (15 days prior feeding following a single necrogenic dose of CCl(4)) of the generation of DNA chain-break and Fe-sugar-base damage assays. The observed hepatoprotective mechanism could be due to its ability to counteract oxidative injury to DNA in the liver of mouse.
Sarkar
A
A
Division of Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Calcutta, 700 032, India.
Pradhan
S
S
Mukhopadhyay
I
I
Bose
S K
SK
Roy
S
S
Chatterjee
M
M
D000208:Acute Disease; D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000235:genetics; D056486:Chemical and Drug Induced Liver Injury; D002869:Chromosome Aberrations; D002908:Chronic Disease; D004247:DNA / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects*; D003855:Deoxyribose / Q000737:chemistry; D000431:Ethanol; D007501:Iron; D008107:Liver Diseases / Q000188:drug therapy*; D019684:Magnoliopsida; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012997:Solvents
D012997:Solvents; D000431:Ethanol; D003855:Deoxyribose; D004247:DNA; D007501:Iron