Local distributions of oviductal estradiol, progesterone, prostaglandins, oxytocin and endothelin-1 in the cyclic cow.
The cyclic patterns of hormones which regulate the activity of the oviduct in the cow have not been adequately reported. We studied progesterone (P4), estradiol 17 beta (E2), prostaglandin E2 (PGE2), prostaglandin F2 alpha (PGF2 alpha), oxytocin (OT) and endothelin-1 (ET-1) concentrations in the cow oviduct. Reproductive tracts from cyclic Holstein cows in the follicular phase (n = 5), post ovulation phase (n = 5) and luteal phase (n = 5) were collected at a slaughterhouse. Oviducts were separated from the uterus, the lumen vas washed with physiological saline, and the enveloping connective tissues were removed. The fimbria was then separated at first and then the rest was divided into 2 parts of equal length (proximal and distal). After extraction, levels of different hormones in the tissues were measured using double antibody enzyme immunoassays (EIAs). There were no differences in any hormone concentration between the 3 parts of the oviduct at any stage of the estrous cycle. The highest concentration of oviductal P4 was observed during the luteal phase and in the oviduct ipsilateral to the functioning CL. Oviductal OT was unchanged throughout the cycle. The highest E2 concentration was observed during the follicular phase in the oviduct ipsilateral to the dominant follicle. The oviduct ipsilateral to the dominant follicle during the follicular phase and ipsilateral to the ovulation site post ovulation showed higher levels of PGE2, PGF2 alpha and ET-1 than those on the contralateral side or during the luteal phase. The highest PGE2 was observed in the oviduct ipsilateral to the ovulation site during the post ovulation phase. The results suggest that the ovarian products (P4, OT and E2) and the local oviductal products (PGE2, PGF2 alpha, and ET-1) may synergistically control oviductal contraction for optimal embryo transport during the periovulatory period, and provide further evidence for the local delivery of ovarian steroids to the adjacent reproductive tract.
Wijayagunawardane
M P
MP
Department of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Miyamoto
A
A
Cerbito
W A
WA
Acosta
T J
TJ
Takagi
M
M
Sato
K
K
D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000033:anatomy & histology* / Q000737:chemistry* / Q000502:physiology; D015237:Dinoprost / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis; D019332:Endothelin-1 / Q000032:analysis; D004958:Estradiol / Q000032:analysis*; D004971:Estrus / Q000502:physiology*; D005187:Fallopian Tubes / Q000737:chemistry / Q000502:physiology; D005260:Female; D010060:Ovulation / Q000502:physiology; D010121:Oxytocin / Q000032:analysis*; D011374:Progesterone / Q000032:analysis*
D019332:Endothelin-1; D011374:Progesterone; D004958:Estradiol; D010121:Oxytocin; D015237:Dinoprost; D015232:Dinoprostone