Production of isopropyl cis-6-hexadecenoate by regiospecific desaturation of isopropyl palmitate by a double mutant of a Rhodococcus strain.
Resting cells of a double mutant noted as KSM-MT66, derived from Rhodococcus sp. strain KSM-B-3 by UV irradiation, were found to cis-desaturate isopropyl hexadecanoate, yielding isopropyl cis-6-hexadecenoate. Addition of sodium glutamate (1.0%), Mg SO4 (2 mM), and thiamine (2 mM) increased the productivity of the unsaturated product in phosphate buffer. Optimal temperature and pH for the reaction were around 26 degrees C and 7, respectively. Under the optimized conditions, more than 50 g/l of isopropyl cis-6-hexadecenoate was produced after a 3-day incubation by resting cells of the mutant. Thus, cis-6-hexadecenoic acid, the main component of human sebaceous lipids, can be manufactured economically by the rhodococcal bioconversion.
Koike
K
K
Tochigi Research Laboratories of Kao Corporation, Haga, Japan.
Takaiwa
M
M
Ara
K
K
Inoue
S
S
Kimura
Y
Y
Ito
S
S
D000596:Amino Acids / Q000378:metabolism; D004952:Esters / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000378:metabolism; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism*; D012240:Rhodococcus / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014815:Vitamins / Q000378:metabolism
D000596:Amino Acids; D004952:Esters; D005229:Fatty Acids, Monounsaturated; D008670:Metals; D010168:Palmitates; D014815:Vitamins; C408379:isopropyl 6-hexadecenoate; C005060:isopropyl palmitate; D010100:Oxygen