Crystallization and preliminary X-ray diffraction studies of phospholipase D from Streptomyces sp.
Crystals of purified phospholipase D (E.C. 3.1.4.4) from Streptomyces sp. strain PMF have been grown under two different crystallization conditions using vapour diffusion. Both conditions gave monoclinic crystals in space group P2(1). The unit-cell parameters were a = 57.28, b = 57.42, c = 68.70 A, beta = 93.17 degrees. The crystals diffract at 110 K to a resolution beyond 1.4 A using synchrotron radiation.
Leiros
I
I
Department of Chemistry, Faculty of Science, University of Tromso, Norway.
Hough
E
E
D'Arrigo
P
P
Carrea
G
G
Pedrocchi-Fantoni
G
G
Secundo
F
F
Servi
S
S
D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010739:Phospholipase D / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D013302:Streptomyces / Q000201:enzymology*
D010739:Phospholipase D