Dependence of elevated human leukocyte antigen class I molecule expression on increased heavy chain, light chain (beta 2-microglobulin), transporter associated with antigen processing, tapasin, and peptide.
Human leukocyte antigen (HLA) class I molecule expression was investigated by DNA-mediated gene transfer. Cell surface expression was increased up to 75% by transfection of HLA-A2 or HLA-B8 heavy chain genes but not genes encoding light chains (beta(2)-microglobulin (beta(2)m)), transporter associated with antigen processing (TAP), or tapasin. Interferon (IFN) treatment further increased expression of transfected heavy chains, suggesting that IFN inducible molecules support heavy chain expression. IFN induces beta(2)m, TAP, and tapasin mRNAs. Transfected heavy chain expression increased upon cotransfection with genes encoding TAP1 and TAP2 but not individual TAP subunits, beta(2)m, or tapasin. Tetracycline inducible heavy chain gene expression was also increased by IFN treatment or TAP cotransfection, suggesting that IFN-induced TAP supports heavy chain maturation. Expression of a mutant that does not interact strongly with TAP, HLA-A2-T134K, was also increased by IFN. Inhibition of TAP-dependent peptide transport by ICP47 reduced heavy chain expression. Expression of HLA-A2, but not HLA-B8, was restored in ICP47 cells by HLA-A2-binding (IP-30) signal peptides. However, these peptides did not further increase transfected HLA-A2 expression, suggesting that peptide availability does not limit heavy chain expression in the absence of ICP47. These results suggest that cytokine-induced TAP supports maturation of HLA class I molecules through combined chaperone and peptide supply functions.
Johnson
D R
DR
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA. david.johnson@yale.edu
Mook-Kanamori
B
B
D017920:Antiporters / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D017931:DNA Primers; D015789:HLA-A2 Antigen / Q000235:genetics*; D015795:HLA-B8 Antigen / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; D021382:Protein Sorting Signals / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D014162:Transfection; D001613:beta 2-Microglobulin / Q000378:metabolism*
D017920:Antiporters; D002352:Carrier Proteins; D017931:DNA Primers; D015789:HLA-A2 Antigen; D015795:HLA-B8 Antigen; D007136:Immunoglobulins; D026901:Membrane Transport Proteins; D021382:Protein Sorting Signals; D011506:Proteins; D001613:beta 2-Microglobulin; C107762:tapasin; D010088:Oxidoreductases; C056859:IFI30 protein, human; D050862:Oxidoreductases Acting on Sulfur Group Donors