title: Protein patterns of gel-entrapped Escherichia coli cells differ from those of free-floating organisms.
abstract: The two-dimensional electrophoretic patterns of cellular proteins from gel-entrapped Escherichia coli cells were compared to those of exponential- and stationary-phase free-floating organisms. The amounts of several proteins in immobilized cells were significantly different from those in free bacteria. Immobilized organisms rapidly produced a high level of dipeptide permease and a single-strand binding protein, and progressively accumulated an aldehyde dehydrogenase. Immobilization also induced a decrease in the levels of two proteins, i.e., the YFID protein and a DNA-binding, stationary-phase protein. The possible role of these proteins in the high resistance of immobilized bacteria to stresses is discussed.
authors.affiliation: UMR 6522 CNRS, Faculté des Sciences de Rouen, Mont-Saint-Aignan, France.
authors.forename: F
authors.initials: F
authors.lastname: Perrot
authors.forename: M
authors.initials: M
authors.lastname: Hébraud
authors.forename: R
authors.initials: R
authors.lastname: Charlionet
authors.forename: G A
authors.initials: GA
authors.lastname: Junter
authors.forename: T
authors.initials: T
authors.lastname: Jouenne
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins