title: Intracerebral injection of autologous whole blood in rats: time course of inflammation and cell death.
abstract: Intracerebral hemorrhage is associated with stroke and head trauma. The purpose of this study was to study brain inflammation and cell death in adult rats 1 h to 4 weeks after injection of blood into the striatum. Terminal dUTP nick-end-labeling positive dying cells were evident 4 h to 4 weeks post-hemorrhage. Neutrophil infiltration was brief and peaked at 48 h. CD8a immunoreactive lymphocytes, possibly natural killer cells, became apparent at 48 h and persisted for 1 week. Microglial reaction was evident at 4 h and persisted for 4 weeks. We conclude that extravascular blood causes a mixed inflammatory cell reaction in brains that is maximal from 48-72 h following hemorrhage. This is associated with death of brain cells over a prolonged period of at least 4 weeks.
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Manitoba, D212-770 Bannatyne Avenue, Winnipeg, Manitoba, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Xue
authors.forename: M R
authors.initials: MR
authors.lastname: Del Bigio
mesh_terms: D000818:Animals; D001804:Blood Transfusion, Autologous / Q000379:methods; D016923:Cell Death; D002465:Cell Movement; D002543:Cerebral Hemorrhage / Q000097:blood* / Q000473:pathology*; D003342:Corpus Striatum / Q000473:pathology; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000097:blood / Q000473:pathology; D007276:Injections, Intraventricular; D007958:Leukocyte Count; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D017628:Microglia / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley