Practical measures to improve in vitro blastocyst production in the bovine.
Media, chemicals, sera and protocols used for in vitro production of bovine embryos are different from one laboratory to another. This paper describes some of the critical steps required to produce embryos in vitro, suggests quality control measures to lower variations in blastocyst yield and describes different tests which may be used when implementing new procedures in a routine production system.
Marquant-Leguienne
B
B
UNCEIA Services Techniques, Maisons Alfort, France.
Humblot
P
P
D000818:Animals; D001755:Blastocyst; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods / Q000592:standards; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000592:standards / Q000662:veterinary*; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D011786:Quality Control; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology