title: Effect of modified dairy fat on fasting and postprandial haemostatic variables in healthy young men.
abstract: It has been suggested that milk fat, due to its content of saturated fatty acids, may have a thrombogenic effect. In the present study the fatty acid profile of milk fat was modified by changing the feeding regimens of cows and the effect on haemostatic variables of a diet containing the modified milk fat (M) was compared with that of a diet containing milk fat of typical Danish composition (D). In the modified fat 16% of the saturated fatty acid (C12-C16) content was replaced mainly by oleic acid. Eighteen subjects were fed on two strictly controlled isoenergetic diets containing 40% energy from total fat (30% energy from the test fats) for periods of 4 weeks in a study with a crossover design. Fasting samples were taken in the last week of each study period. Postprandial samples were taken on day 21, 3 h after lunch (n 18), and on the last day of the study 2, 4, 6 and 8 h after a fat load containing 1.2 g of one of the milk fats/kg body weight (n 8). After 4 weeks' dietary intervention fasting plasma factor VII coagulant (FVIIc) activity, tissue-type plasminogen activator (t-PA) activity, plasminogen activator inhibitor (PAI-1) antigen and beta-thromboglobulin did not differ between diets M and D. Postprandially FVIIc and t-PA activities increased (P < 0.001) and PAI-1 antigen and PAI-1 activity decreased (P < 0.001) as compared with fasting values, regardless of diet. After the fat load, the postprandial increase in FVIIc was marginally lower after diet M than diet D (diet effect, P < 0.05). In conclusion, the modified milk fat obtained by the applied feeding strategy had virtually the same effects on haemostatic variables as conventional milk fat.
authors.affiliation: Research Department of Human Nutrition, Royal Veterinary and Agricultural University, Frederiksberg, Denmark. TineTholstrup@fhe.kvl.dk
authors.forename: T
authors.initials: T
authors.lastname: Tholstrup
authors.forename: P
authors.initials: P
authors.lastname: Marckmann
authors.forename: J
authors.initials: J
authors.lastname: Hermansen
authors.forename: G
authors.initials: G
authors.lastname: Hølmer
authors.forename: B
authors.initials: B
authors.lastname: Sandström
mesh_terms: D000328:Adult; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002417:Cattle; D018592:Cross-Over Studies; D005167:Factor VII / Q000032:analysis; D005215:Fasting / Q000097:blood; D005227:Fatty Acids / Q000032:analysis*; D005526:Food, Formulated; D006487:Hemostasis; D006801:Humans; D008297:Male; D008892:Milk / Q000737:chemistry*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D019518:Postprandial Period; D010959:Tissue Plasminogen Activator / Q000097:blood; D014280:Triglycerides / Q000097:blood*; D001620:beta-Thromboglobulin / Q000032:analysis
chemical_list: D005227:Fatty Acids; D017395:Plasminogen Activator Inhibitor 1; D014280:Triglycerides; D001620:beta-Thromboglobulin; D005167:Factor VII; D010959:Tissue Plasminogen Activator