Growth behaviour and indole acetic acid (IAA) production by a Rhizobium isolated from root nodules of Alysicarpus vaginalis DC.
From the root nodules of Alysicarpus vaginalis DC, the symbiont was isolated and identified as a Rhizobium sp. The bacteria produced a high amount (107 microg/ml) of indole acetic acid (IAA) in culture from tryptophan supplemented yeast extract mannitol medium. The isolate preferred L-isomer of tryptophan for maximum IAA production. The production was maximum when the bacteria reached its stationary phase of growth. The production of IAA could be increased up to 70% over yeast extract glucose medium by supplementing ZnSO4, 7H2O (0.5 microg/ml). L-asparagine (0.2%) and sodium dodecyl sulfate (1.0 microg/ml). The possible relationship between the rhizobial IAA production and legume-rhizobia symbiosis is discussed.
Bhattacharyya
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P.G. Department of Botany, Darjeeling Government College, West Bengal, India.
Pati
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D002244:Carbon / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media; D007887:Fabaceae / Q000382:microbiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D007536:Isomerism; D009584:Nitrogen / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology*; D010946:Plants, Medicinal; D012231:Rhizobium / Q000254:growth & development* / Q000302:isolation & purification* / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
D003470:Culture Media; D007210:Indoleacetic Acids; C030737:indoleacetic acid; D002244:Carbon; D014364:Tryptophan; D009584:Nitrogen