title: Interaction of the Neurospora crassa heat shock factor with the heat shock element during heat shock and different developmental stages.
abstract: The interaction of the heat shock factor (HSF) with the heat shock element (HSE) was determined by a non-radioactive electrophoretic mobility shift assay, in order to analyze HSF regulation in Neurospora crassa. HSF binds to HSE under normal, non-stress conditions and is thus constitutively trimerized. Upon heat shock, the HSF-HSE complex shows a retarded mobility. This was also observed in Saccharomyces cerevisiae, where this mobility shift was shown to be due to HSF phosphorylation [Sorger and Pelham (1988) Cell 54, 855-864]. In N. crassa, HSE-dependent electrophoretic mobility shift is temperature- and time-dependent. Under normal growth conditions, the HSF is located in the cytoplasm as well as in the nucleus. In germinating conidia the HSF shows a retarded mobility typical for heat shock even at normal growth temperatures. No HSF-dependent mobility shift was detectable in aerial hyphae.
authors.affiliation: Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, Leobener Strasse, NW2, D-28359, Bremen, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Meyer
authors.forename: C
authors.initials: C
authors.lastname: Monnerjahn
authors.forename: D
authors.initials: D
authors.lastname: Techel
authors.forename: L
authors.initials: L
authors.lastname: Rensing
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C067168:HSF1 protein, S cerevisiae; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors