The steady-state levels of oxidative DNA damage and of lipid peroxidation (F2-isoprostanes) are not correlated in healthy human subjects.
Oxidative damage to DNA in human tissues can be determined by measuring multiple products of oxidative damage to the purine and pyrimidine bases using gas chromatography-mass spectrometry (GC-MS). Oxidative damage to lipids (lipid peroxidation) can be quantitated by the mass spectrometry-based determination of F2-isoprostanes, specific end-products of the peroxidation of arachidonic acid residues in lipids. For both DNA base damage products and 8-epi prostaglandin F2alpha (PGF2alpha), there is a wide variation in levels between different healthy human subjects. We measured multiple products of oxidative damage to DNA bases in white cells, and 8-epi PGF2alpha in plasma, from blood samples obtained from healthy human subjects in the UK and in Portugal. No correlation of 8-epi PGF2alpha levels with levels of any modified DNA base (including 8-hydroxyguanine) was observed. We conclude that no single parameter can be measured as an index of "oxidative stress" or "oxidative damage" in vivo.
England
T
T
International Antioxidant Research Centre, King's College, Guys Campus, London, UK.
Beatty
E
E
Rehman
A
A
Nourooz-Zadeh
J
J
Pereira
P
P
O'Reilly
J
J
Wiseman
H
H
Geissler
C
C
Halliwell
B
B
D000328:Adult; D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism; D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011174:Portugal; D006113:United Kingdom
C075750:8-epi-prostaglandin F2alpha; D016718:Arachidonic Acid; C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA; D015237:Dinoprost