Selective interactions between epithelial tumour cells and bone marrow mesenchymal stem cells.
This work is a comparative study on the features displayed by an epithelial metastatic breast cancer cell line (MCF-7) when set in co-culture with human bone marrow mesenchymal stem cells (MSC) or a feeder layer of 3T3 fibroblasts. MSC, a subset of non-haematopoietic cells in the marrow stroma, display a potential for self-renewal, proliferation and differentiation into precursors for bone, cartilage, connective and muscular tissue. Adhesion of MCF-7 cells to monolayers of MSC or 3T3 was high (95 and 85% respectively). Once attached, MCF-7 grow well on both monolayers. Morphology of MCF-7 cells, as analysed by light and epifluorescence microscopy, revealed that MCF-7 cells grow in clusters on 3T3, but disperse on MSC. Concomitant with the lost of their aggregation status, MCF-7 on MSC express low levels of the intercellular adhesion molecules, E-cadherin and epithelial-specific antigen (ESA). These results suggest that MSC represent an appropriate cell target to investigate the cellular and molecular events occurring at the interface of epithelial-marrow stromal interactions. Together, the model here described should permit to further evaluate the significance and prognostic impact of the shift of micrometastatic cells from a cluster-aggregated into a single-cell status.
Hombauer
H
H
Unidad de Biología Celular, INTA, Universidad de Chile, Santiago.
Minguell
J J
JJ
D001943:Breast Neoplasms / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002449:Cell Aggregation; D002450:Cell Communication; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D014407:Tumor Cells, Cultured / Q000502:physiology
D015815:Cell Adhesion Molecules