title: Effect of Shiga toxin 2 on water and ion transport in human colon in vitro.
abstract: Shiga toxin-producing Escherichia coli (STEC) colonize the lower segments of the human gastrointestinal tract, causing gastrointestinal and systemic diseases. In this study, the effects of Shiga toxin 2 (Stx2) on fluid absorption and ion transport in the human colon were examined. Net water movement (Jw) and short-circuit current (Isc) were simultaneously measured across the colonic mucosa incubated with crude or purified Stx2. Stx2 significantly inhibited the absorptive J(w) with no effect on the basal I(sc) after 60 min of exposure. These effects may be due to the inhibition of a nonelectrogenic transport system present in the surface colonic villus cells. Morphological studies of the colonic mucosa treated with crude or purified Stx2 demonstrated a selective damage in the absorptive villus epithelial cells. These findings suggest that Stx2 inhibits water absorption across the human colon by acting on a specific cell population: the mature, differentiated absorptive villus epithelium.
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Fiorito
authors.forename: J M
authors.initials: JM
authors.lastname: Burgos
authors.forename: M F
authors.initials: MF
authors.lastname: Miyakawa
authors.forename: M
authors.initials: M
authors.lastname: Rivas
authors.forename: G
authors.initials: G
authors.lastname: Chillemi
authors.forename: D
authors.initials: D
authors.lastname: Berkowski
authors.forename: E
authors.initials: E
authors.lastname: Zotta
authors.forename: C
authors.initials: C
authors.lastname: Silberstein
authors.forename: C
authors.initials: C
authors.lastname: Ibarra
mesh_terms: D001427:Bacterial Toxins / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D004768:Enterotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D017136:Ion Transport / Q000187:drug effects*; D022603:Shiga Toxins; D014867:Water / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D022603:Shiga Toxins; D014867:Water