title: [Rapid microdose 14C-urea breath test for detection of Helicobacter pylori infection].
abstract: The purpose of this study was to develop and evaluate a rapid microdose 14C-urea breath test (14C-UBT) with a simplified protocol for detecting the infection of hilicobacter pylori (HP). 157 fasting patients who underwent endoscopy with histological examination and rapid urease test (RUT) were given a drink of 37 kBq of 14C-urea. Samples of breath carbon dioxide (1 mmol) were collected at baseline and 10, 20 and 30 min after administration by trapping in hyamine solution. 14C activity was measured by liquid scintillation counting. Results were expressed as cpm. Histolal examination and RUT were used as gold standard for the detection of HP infection. The cutoff value was selected as 200 cpm at 10 min. The results showed that the sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 94.79%, 90.16%, 93.81%, 91.60% and 92.99% respectively. In this study, a 10 min, single sample, 37 kBq 14C-urea breath test for detection HP was developed. The test has good diagnostic accuracy with minimal radiation exposure and low cost. Thus, the authors considered the test to be reliable, safe, convenient and cost-effective to clinical use.
authors.affiliation: Department of Nuclear Medicine, First Affiliated Hospital, Chengdu.
authors.forename: A
authors.initials: A
authors.lastname: Kuang
authors.forename: Z
authors.initials: Z
authors.lastname: Liang
authors.forename: T
authors.initials: T
authors.lastname: Tan
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Ma
authors.forename: K
authors.initials: K
authors.lastname: Si
authors.forename: Q
authors.initials: Q
authors.lastname: Ouyang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001944:Breath Tests; D002250:Carbon Radioisotopes; D004381:Duodenal Ulcer / Q000382:microbiology*; D005260:Female; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D013276:Stomach Ulcer / Q000382:microbiology; D014508:Urea
chemical_list: D002250:Carbon Radioisotopes; D014508:Urea