A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria.
A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required.
McKerrow
J
J
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria, Australia.
Vagg
S
S
McKinney
T
T
Seviour
E M
EM
Maszenan
A M
AM
Brooks
P
P
Seviour
R J
RJ
D002473:Cell Wall / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003960:Diaminopimelic Acid / Q000032:analysis*; D006094:Gram-Positive Bacteria / Q000737:chemistry*
D003960:Diaminopimelic Acid