title: Effect of exogenous melatonin on the ovarian follicles in gamma-irradiated mouse.
abstract: The present study was performed to obtain the evidence of the radioprotective function of melatonin on gamma-radiation-induced follicular atresia in mouse ovary. Three-week-old immature mice received 10 and 100 microg of melatonin dissolved in 100 microl of the alcoholic saline. Two hours after the treatments, they were whole-body irradiated with a dose of LD(80(30)) (8.3 Gy). The ovaries were dissected out of the animals at -2, 2, 8, 14 h after the onset of irradiation. The total number of follicles including the normal and atretic follicles examined in the largest cross sections was 125. The number was reduced to 103 in the irradiated group. The number of primordial follicles of the irradiation group or the melatonin-treated group was smaller than that of the control group. However, the number of primary, preantral, and antral follicles was not different from that of the control group. In the group pretreated with 100 microg of melatonin before irradiation, the ratio of normal primordial follicles was significantly higher than that of the irradiation group at any time point after irradiation. The high concentration of melatonin also reduced the radiation-induced degeneration of the primary follicles at 14 h after irradiation. On the other hand, the pretreatment of 10 microg of melatonin had little or no effect on the radiation-induced degeneration of primary follicles. However, it gave a protective effect on the radiation-induced degeneration in the primordial follicles at 2 h after irradiation, and 14 h after irradiation in preantral and antral follicles. From the above results, it is concluded that the exogenous melatonin has different functions depending on the follicle stages, and that the radioprotective effect of exogenous melatonin on the follicular degeneration is related to its concentration.
authors.affiliation: Korea Atomic Energy Research Institute, 150 Dukjin-dong, Yusong-gu, Taejon, South Korea. jkkim@nanum.kaeri.re.kr
authors.forename: J K
authors.initials: JK
authors.lastname: Kim
authors.forename: C J
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D005260:Female; D005720:Gamma Rays; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D006080:Ovarian Follicle / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
chemical_list: D011837:Radiation-Protective Agents; D008550:Melatonin