Immunoglobulin in atherosclerotic lesions of human aorta.
An augmented expression of mRNA for IgG light chain was demonstrated age-dependently on atheromatous lesions of the aorta in Watanabe heritable hyperlipidemic (WHHL) rabbits. The present study was designed to determine factors related to inflammation in human vessels excised during surgery. We detected IgG mRNAs using RT-PCR in human atherosclerotic lesions but not in human umbilical arteries which have no atheromatous lesions. To determine the clonality of IgGs, cDNAs encoding variable regions of IgG heavy chain were examined using RT-PCR. Atherosclerotic lesions had several subtypes of IgG gene families' suggesting the involvement of polyclonal B-cells. mRNAs of interleukins-6 (IL-6), -1alpha (IL-1alpha), and -1beta (IL-1beta) were also detected in the same samples. In summary, inflammatory reactions were present in the atherosclerosis lesion.
Shiga
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First Department of Internal Medicine, Yamagata University School of Medicine, Yamagata-shi, Japan.
D000368:Aged; D000818:Animals; D001011:Aorta / Q000737:chemistry*; D001161:Arteriosclerosis / Q000378:metabolism*; D005260:Female; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000097:blood* / Q000235:genetics; D007375:Interleukin-1 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007147:Immunoglobulin Light Chains; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger