title: Evaluation of fluids from cystic follicles for in vitro maturation and fertilization of bovine oocytes.
abstract: Follicular cysts are defined as cystic structures derived from unovulated follicles. The formation of the cysts appears to be related to failure of the oocyte to resume meiosis. The aim of this study was to evaluate in the bovine: 1) the ability of the fluid from cystic follicles to promote in vitro oocyte maturation and fertilization, 2) the predictive value of the morphology of oocytes derived from cystic follicles on the ability of the follicular fluid to promote in vitro maturation/fertilization as well as the oocytes to undergo maturation and fertilization. In Experiment 1, the ability of fluid from cystic (and normal) follicles from live and slaughtered cows (to promote) in vitro maturation and fertilization of bovine cumulus-oocyte-complexes (COC's) was assessed by cumulus expansion, sperm penetration, male pronucleus formation and polyspermy rates. Concentrations of progesterone (P4) and estradiol-17 beta (E2) were measured in the fluid from cystic follicles collected from live and slaughtered cows. In Experiment 2, we investigated the relationship of the morphology of COC's from cystic follicles, and the effect of the follicular fluids on oocyte maturation as well as P4 and E2 concentrations. In Experiment 1, although sperm penetration and male pronucleus formation were inhibited significantly by fluid from some cystic follicles collected from live and slaughtered cows, there were no significant differences in sperm penetration, male pronucleus formation and polyspermy rates between fluid from cystic follicles collected from live cows, from slaughtered cows and from control groups, regardless of the P4/E2 ratio. In Experiment 2, the morphology of cumulus-oocyte complexes from cystic follicles varied and the pronucleus formation of oocytes after in vitro fertilization was abnormal. On the other hand, the male pronucleus formation rates were not significantly different between the cystic follicular fluids and control, regardless, of the P4/E2 ratio. The results of this study suggest that many of the bovine follicular fluids from cystic follicles possess the ability to induce cumulus expansion, nuclear maturation and male pronucleus formation following in vitro maturation and fertilization of bovine oocytes. The morphology of the cumulus-oocytes complexes from cystic follicles seems not to relate to the ability of the cystic follicular fluids to induce oocyte maturation, and oocytes from cystic follicles possess the ability to form male pronucleus even though most were abnormal after in vitro fertilization.
authors.affiliation: Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: Y H
authors.initials: YH
authors.lastname: Choi
authors.forename: H
authors.initials: H
authors.lastname: Kamishita
authors.forename: M
authors.initials: M
authors.lastname: Ohtani
authors.forename: T J
authors.initials: TJ
authors.lastname: Acosta
authors.forename: M P
authors.initials: MP
authors.lastname: Wijayagunawardane
authors.forename: A
authors.initials: A
authors.lastname: Miyamoto
authors.forename: K
authors.initials: K
authors.lastname: Miyazawa
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: E
authors.initials: E
authors.lastname: Sato
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005497:Follicular Cyst / Q000503:physiopathology*; D015571:Follicular Fluid / Q000502:physiology*; D008297:Male; D009865:Oocytes / Q000254:growth & development*; D013084:Sperm-Ovum Interactions