Contributions of immunohistochemistry and in situ hybridization to the functional analysis of pituitary adenomas.
Immunohistochemistry (IHC) and recently in situ hybridization (ISH) have elucidated various aspects of human pituitary adenomas, i.e., functional differentiation and classification, transcription factors and mechanism of hormone production, regulation of hormone secretion, and processing of prohormones. Recently, the use of tyramide (catalyzed signal amplification; TSA or CSA) and RT-PCR has been effective for detection of trivial amount of proteins (peptides) and mRNA, respectively. Immunomolecular histochemistry is expected to further clarify the function and biology of human pituitary adenomas.
Osamura
R Y
RY
Department of Pathology, Tokai University School of Medicine, Boseidai Isehara-city, Kanagawa, Japan.
Tahara
S
S
Kurotani
R
R
Sanno
N
N
Matsuno
A
A
Teramoto
A
A
D000236:Adenoma / Q000145:classification / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D010902:Pituitary Gland / Q000378:metabolism; D010907:Pituitary Hormones / Q000096:biosynthesis / Q000378:metabolism; D010911:Pituitary Neoplasms / Q000145:classification / Q000378:metabolism* / Q000503:physiopathology; D014157:Transcription Factors / Q000378:metabolism
D010907:Pituitary Hormones; D014157:Transcription Factors