[The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability].
Karyotypic variability has been investigated for nonimmortalized human embryonic lung cell line MRC-5, cultivated with Acholeplasma laidlawii strain PG-8 for 15-45 days. The character of cell distribution for chromosome number did not change during this time. In all investigated variants the number of polyploid cells increased considerably with the lengthening of the term after decryoconservation. The number of chromosomal aberrations in 15-45 days contaminated cells increased significantly as compared to the control at the expense of dicentrics (telomeric associations). The number of dicentrics had a tendency to increase with the lengthening of the term of contamination. Thus, in 45 days the number of dicentrics increased twice as much as that in 15 days. The increase of polyploids may be due presumably to the specific character of karyotypic variability in nonimmortalized cell lines with the long-term cultivation. Our present and previous results made it possible to suppose that the formation of dicentrics (telomeric associations) in nonimmortalized "markerless" cell line, following the long-term mycoplasmal contamination, may prove additionally the role played by dicentrics in cell adaptation to in vitro conditions whatever the degree of transformation may be--nonimmortalized line or immortalized nontumorogenic or high tumorogenic lines.
Polianskaia
G G
GG
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Efremova
T N
TN
Sakuta
G A
GA
D000128:Acholeplasma laidlawii; D002460:Cell Line; D002869:Chromosome Aberrations; D014644:Genetic Variation; D006801:Humans; D007621:Karyotyping; D008168:Lung / Q000382:microbiology