Capillary zone electrophoretic separation and determination of imidazolic antifungal drugs.
Capillary zone electrophoresis (CZE) was adapted to the simultaneous determination of a mixture of three imidazolic antifungal drugs. Separation was achieved by using a fused-silica capillary column with an acetic acid-Tris buffer at pH 5.18 and UV detection at 196 nm. Several electrophoretic parameters were investigated: pH and buffer concentration, applied voltage, temperature and injection conditions. The optimized CZE method was applied to the individual determination of ketoconazole, clotrimazole and econazole in pharmaceutical forms, after a previous single extraction step in methanol, with recoveries of 98.00, 99.96 and 99.58% respectively. The antifungal drugs can be determined at a concentration level lower than 1.0 x 10(-7) M.
Arranz
A
A
Departamento de Química Analítica, Facultad de Farmacia, Universidad del País Vasco/EHU, Vitoria, Spain. qaparvaj@vc.ehu.es
Echevarría
C
C
Moreda
J M
JM
Cid
A
A
Arranz
J F
JF
D000935:Antifungal Agents / Q000032:analysis / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007093:Imidazoles / Q000032:analysis / Q000302:isolation & purification*
D000935:Antifungal Agents; D007093:Imidazoles