title: In vivo evidence that erythropoietin has a neuroprotective effect during subarachnoid hemorrhage.
abstract: To ascertain in vivo whether recombinant human erythropoietin has a neuroprotective effect on the cortex during subarachnoid hemorrhage, 56 rabbits were divided into the following groups: Group 1 control sham operated plus placebo (n=14; saline solution - NaCl 0.9%); Group 2 control sham operated plus recombinant human erythropoietin (n=14); Group 3 subarachnoid hemorrhage plus placebo (n=14); Group 4 subarachnoid hemorrhage plus recombinant human erythropoietin (n=14; intraperitoneal administration of recombinant human erythropoietin immediately after inducing subarachnoid hemorrhage). In none of the Groups 1 and 2 animals was subarachnoid hemorrhage induced. In Group 3 rabbits, an increase in locomotor activity (open field apparatus) was observed 24, 48 and 72 h after surgery, and the mortality rate was 42.9% within 72 h after surgery, and, no increase in locomotor activity was observed in Group 4 rabbits, which survived for at least 72 h. Our findings suggest that recombinant human erythropoietin may be of benefit in the treatment of subarachnoid hemorrhage.
authors.affiliation: Department of Internal Medicine, University of Messina, Messina, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Buemi
authors.forename: G
authors.initials: G
authors.lastname: Grasso
authors.forename: F
authors.initials: F
authors.lastname: Corica
authors.forename: G
authors.initials: G
authors.lastname: Calapai
authors.forename: F M
authors.initials: FM
authors.lastname: Salpietro
authors.forename: T
authors.initials: T
authors.lastname: Casuscelli
authors.forename: A
authors.initials: A
authors.lastname: Sfacteria
authors.forename: C
authors.initials: C
authors.lastname: Aloisi
authors.forename: C
authors.initials: C
authors.lastname: Alafaci
authors.forename: A
authors.initials: A
authors.lastname: Sturiale
authors.forename: N
authors.initials: N
authors.lastname: Frisina
authors.forename: F
authors.initials: F
authors.lastname: Tomasello
mesh_terms: D000818:Animals; D004921:Erythropoietin / Q000627:therapeutic use*; D005260:Female; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D011817:Rabbits; D011994:Recombinant Proteins; D013345:Subarachnoid Hemorrhage / Q000188:drug therapy*
chemical_list: D018696:Neuroprotective Agents; D011994:Recombinant Proteins; D004921:Erythropoietin