title: Cell populations in lesions of cutaneous leishmaniasis of Leishmania (L.) amazonensis- infected rhesus macaques, Macaca mulatta.
abstract: The cellular nature of the infiltrate in cutaneous lesion of rhesus monkeys experimentally infected with Leishmania (L.) amazonensis was characterized by immunohistochemistry. Skin biopsies from infected animals with active or healing lesions were compared to non-infected controls (three of each type) to quantitate inflammatory cell types. Inflammatory cells (composed of a mixture of T lymphocyte subpopulations, macrophages and a small number of natural killer cells and granulocytes) were more numerous in active lesions than in healing ones. T-cells accounted for 44.7 +/- 13.1% of the infiltrate in active lesions (versus CD2+ = 40.3 +/- 5.7% in healing lesions) and T-cell ratios favor CD8+ cells in both lesion types. The percentage of cells expressing class II antigen (HLA-DR+) in active lesions (95 +/- 7.1%) was significantly higher (P < 0.005) from the healing lesions (42.7 +/- 12.7%). Moreover, the expression of the activation molecules CD25 (@ 16%), the receptor for interleukin-2, suggests that many T cells are primed and proliferating in active lesions. Distinct histopathological patterns were observed in lesions at biopsy, but healing lesions contained more organized epithelioid granulomas and activated macrophages, followed by fibrotic substitution. The progression and resolution of skin lesions appears to be very similar to that observed in humans, confirming the potential for this to be used as a viable model to study the immune response in human cutaneous leishmaniasis.
authors.affiliation: Departamento de Imunologia, Fundação do Instituto Oswaldo Cruz, Rio de Janeiro, RJ, 21045-900, Brasil.
authors.forename: V
authors.initials: V
authors.lastname: Amaral
authors.forename: C
authors.initials: C
authors.lastname: Pirmez
authors.forename: A
authors.initials: A
authors.lastname: Gonçalves
authors.forename: V
authors.initials: V
authors.lastname: Ferreira
authors.forename: G
authors.initials: G
authors.lastname: Grimaldi
mesh_terms: D000818:Animals; D001706:Biopsy; D016022:Case-Control Studies; D004195:Disease Models, Animal; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology; D007111:Immunity, Cellular; D007150:Immunohistochemistry; D007894:Leishmania mexicana / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D008253:Macaca mulatta; D008297:Male; D012882:Skin Tests; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens