Fine specificity of anti-Le(X) monoclonal antibody TEC-01.
TEC-01 monoclonal antibody recognizes the oligosaccharide structure Galbeta1-->4[Fucalpha1-->3] GlcNAc (Le(X) hapten). To determine its fine specificity, reactivity of TEC-01 antibody with Le(X) glycosphingolipids, isolated from human liver metastasis of adenocarcinoma, and Le(X) neoglycolipid were analysed. Immunostaining of Le(X) glycosphingolipids, fractionated by thin-layer chromatography, showed that TEC-01 reacted with difucosyl and trifucosyl Le(X) glycosphingolipids but not with Le(X) pentasaccharide ceramide. Interestingly, TEC-01 also reacted with Le(X) neoglycolipid prepared by reductive amination from Le(X) pentasaccharide, lacto-N-fucopentaose III, and L-1,2-dipalmitoyl-sn-glycerophosphoethanolamine. The combined data suggest that TEC-01 recognizes Le(X) hapten in glycolipids with longer oligosaccharide chain moiety.
Bohata
J
J
1st Department of Medicine, 1st Medical Faculty, Charles University, Prague, Czech Republic.
Smíd
F
F
Dráber
P
P
D000230:Adenocarcinoma / Q000737:chemistry / Q000276:immunology / Q000556:secondary; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D006028:Glycosphingolipids / Q000737:chemistry / Q000276:immunology; D006241:Haptens / Q000737:chemistry; D006801:Humans; D016256:Lewis X Antigen / Q000737:chemistry / Q000276:immunology*; D008113:Liver Neoplasms / Q000737:chemistry / Q000276:immunology / Q000556:secondary; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D006241:Haptens; D016256:Lewis X Antigen; D009844:Oligosaccharides