title: Separation of related opiate compounds using capillary electrochromatography.
abstract: Capillary electrophoretic separations have been investigated for six controlled narcotic analgesic compounds having related structures. Owing to the similar charge-to-mass ratios of these compounds, capillary zone electrophoresis failed to provide a satisfactory separation, whereas a baseline-resolved separation was achieved in 10 min using micellar electrokinetic chromatography. Column efficiencies of 40,000-150,000 plates/m were obtained with a 50 cm long, 50 microm inner diameter (ID) capillary using 50 mM sodium dodecyl sulfate (SDS) in a 50 mM borate solution containing 12% isopropanol. In contrast, separation of this mixture by capillary electrochromatography proved to be significantly superior. The capillary was 15 cm long, with an ID of 75 microm, and was packed with 1.5 microm nonporous octadecyl silica (ODS) particles. The mobile phase consisted of 80% 10 mM tris(hydroxymethyl)aminomethane (Tris) and 20% acetonitrile, and contained 5 mM SDS. A complete separation was obtained in 2.5 min with an efficiency of 250,000-500,000 plates/m.
authors.affiliation: Department of Chemistry, Stanford University, CA 94305, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Lim
authors.forename: R N
authors.initials: RN
authors.lastname: Zare
authors.forename: C G
authors.initials: CG
authors.lastname: Bailey
authors.forename: D J
authors.initials: DJ
authors.lastname: Rakestraw
authors.forename: C
authors.initials: C
authors.lastname: Yan
mesh_terms: D000701:Analgesics, Opioid / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D007202:Indicators and Reagents; D009294:Narcotics / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000701:Analgesics, Opioid; D007202:Indicators and Reagents; D009294:Narcotics