Analysis of ochratoxin A in pig kidney and rye flour using liquid chromatography tandem mass spectrometry (LC/MS/MS).
A liquid chromatography electrospray tandem mass spectrometric (LC/MS/MS) method is described for analysis and confirmation of ochratoxin A in pig kidney and rye flour using derivatization of ochratoxin A to the methyl ester. Ochratoxin A methyl(d3)ester is used as internal standard. The method works well, the detection limit is 0.02 microgram/kg and the repeatability (coefficient of variation) is between 6% and 16% in the contamination range 0.5 to 8 micrograms/kg.
Jørgensen
K
K
Institute of Food Research and Nutrition, Danish Veterinary and Food Administration, Soborg, Denmark. kej@vfd.dk
Vahl
M
M
D000818:Animals; D002273:Carcinogens / Q000032:analysis; D002853:Chromatography, Liquid; D005433:Flour / Q000032:analysis*; D005506:Food Contamination; D006801:Humans; D007668:Kidney / Q000737:chemistry; D013058:Mass Spectrometry; D008460:Meat / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D012434:Secale / Q000737:chemistry; D013552:Swine
D002273:Carcinogens; D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A