Selective isolation of eae-positive strains of shiga toxin-producing Escherichia coli.
Culture on cefixime, tellurite, and sorbitol-MacConkey agar after HCl treatment facilitated the growth of 410 (94%) of 436 eae-positive Shiga toxin-producing Escherichia coli (STEC) strains and 17 (16%) of 107 eae-negative STEC strains. This selectivity was closely related to acid resistance in E. coli and tellurite resistance in eae-positive STEC strains.
Fukushima
H
H
Public Health Institute of Shimane Prefecture, Matsue, Shimane 690-0122, Japan. hiroshi@joho-shimane.or.jp
Hoshina
K
K
Gomyoda
M
M
D018829:Adhesins, Bacterial; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002352:Carrier Proteins; D002417:Cattle; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D022603:Shiga Toxins; D013691:Tellurium / Q000494:pharmacology; D014774:Virulence
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D022603:Shiga Toxins; C073819:eaeA protein, E coli; C026660:tellurous acid; D013691:Tellurium; D006851:Hydrochloric Acid