[A molecular cytogenetic study on chromosome anomalies of chronic myeloid leukemia].
OBJECTIVE
To study the bcr/abl fusion gene in chronic myeloid leukemia (CML) cases with Philadelphia chromosome(Ph) negative, and variant Ph translocations and other chromosome anomalies.


METHODS
The status of bcr/abl fusion gene in 8 kinds of chromosome anomalies was tested with dual-color fluorescence in situ hybridization (D-FISH) technique.


RESULTS
The positive hybridization was demonstrated in 3 cases of variant Ph CML and other 7 cases of standard Ph, respectively. In 2 cases of Ph-negative CML, one case that had karyotype such as 46,XY,t(9;18) showed positive hybridization, the other case with 46,XY,inv(2) showed negative result.


CONCLUSIONS
The result demonstrates that D-FISH technique is reliable for detecting bcr/abl fusion gene. The existence of negative bcr/abl fusion gene CML implies that some other initial events inducing CML might have occurred in such cases.
Zhu
B
B
Medical Genetics Center of Yunnan Province, The First Provincial Hospital of Yunnan, Kunming, Yunnan, 650032 P.R. China. bszhu@public. km. yn.cn.
Shen
X
X
Li
C
C
Lai
X
X
Li
Y
Y
Shi
K
K
Feng
H
H
Wang
R
R
D000328:Adult; D002869:Chromosome Aberrations; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged
D016044:Fusion Proteins, bcr-abl