Detection of Ureaplasma diversum in cattle using a newly developed PCR-based detection assay.
Ureaplasma diversum has been associated with different clinical manifestations including bovine vulvitis, endometritis, salpingitis, spontaneous abortion and infertility. Because the isolation of this ureaplasma from clinical samples is difficult, there is a need for improved detection methods. We developed a PCR assay based on amplification of a region of the gene encoding 16S rRNA. The specificity of the amplification was verified by sequence analysis. Female bovine vaginal swabs (n=168) were collected and the presence of U. diversum evaluated by both culture methods and by the PCR assay. Culture was positive for 60 samples (35.7%), and PCR-specific amplification was obtained for 89 samples (52.9%). These results indicated a high prevalence of U. diversum in the selected animals and the higher sensitivity of this PCR assay as compared to culture.
Vasconcellos Cardoso
M
M
Laboratório de Doenças Bacterianas da Reprodução, Centro de Sanidade Animal, Instituto Biológico, São Paulo, Brazil.
Blanchard
A
A
Ferris
S
S
Verlengia
R
R
Timenetsky
J
J
Florio Da Cunha
R A
RA
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D005779:Immunodiffusion / Q000662:veterinary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014509:Ureaplasma / Q000235:genetics / Q000302:isolation & purification*; D016869:Ureaplasma Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D014621:Vagina / Q000382:microbiology; D014847:Vulvitis / Q000209:etiology / Q000662:veterinary
D000907:Antibodies, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S