[Preparative dephosphorylation of calcium salts of 2'(3)-mononucleotides by extracellular phosphatase from Spicaria violacea].
The properties of the phosphatase present in the culture liquid of Spicaria violacea were investigated. Based on these results, a method for preparative dephosphorylation of calcium salts of 2'(3')-mononucleotides was proposed. A 96-98% yield was achieved at a substrate concentration of 100 mg/ml. Mild quantitative hydrolysis of RNA to nucleosides can be performed by RNA digestion to mononucleotides with Ca2+ followed by the proposed dephosphorylation procedure.
Baraĭ
V N
VN
Institute of Microbiology, National Academy of Sciences of Belarus.
Severina
I A
IA
Kukharskaia
T A
TA
Zinchenko
A I
AI
D000249:Adenosine Monophosphate / Q000378:metabolism*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010766:Phosphorylation
D005656:Fungal Proteins; D000249:Adenosine Monophosphate; D010744:Phosphoric Monoester Hydrolases