Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1.
Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specificity of amplification.
Cândido
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Laboratório de Virologia Comparada, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 30161-970, Brasil.
Bontempo
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Resende
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D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000032:analysis; D016679:Genome, Viral; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012997:Solvents; D013045:Species Specificity
D017931:DNA Primers; D012997:Solvents