In vitro airway responsiveness of Flinders sensitive and resistant line rats.
Recently, we reported that freely moving Flinders sensitive line rats (FSL, selectively bred for their cholinergic hyperresponsiveness) are more susceptible to allergen-induced airway hyperresponsiveness than their control counterparts-Flinders resistant line (FRL) rats. In this study the two Flinders lines were compared for responsiveness of excised tracheal and primary bronchial smooth muscle in vitro. FSL tissues were slightly but significantly more sensitive to cholinergic stimulation than FRL tissues (slightly lower EC(50) value for carbachol) but the FRL tissues were more responsive, exhibiting larger amplitude of response. Surprisingly, previous exposure to allergen challenge was accompanied by reduced in vitro responses to spasmogens in both rat lines. We conclude that FSL and FRL airways do not differ greatly with respect to sensitivity to cholinergic stimulation in vitro and that inflamed airways show reduced in vitro responses to spasmogens. The discrepancy between the in vivo and in vitro findings suggests that responsiveness of airway smooth muscle involves regulation from both proximal and distal sites.
Janssen
L J
LJ
Department of Medicine, McMaster University.
Djurić
V J
VJ
Wattie
J
J
Otis
J
J
O'Byrne
P M
PM
D000403:Airway Resistance / Q000187:drug effects / Q000235:genetics*; D000485:Allergens / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001980:Bronchi / Q000187:drug effects; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000235:genetics*; D016084:Bronchoconstriction / Q000187:drug effects / Q000235:genetics*; D016085:Bronchoconstrictor Agents / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D018678:Cholinergic Agents / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D009130:Muscle, Smooth / Q000187:drug effects / Q000294:innervation; D010047:Ovalbumin / Q000494:pharmacology / Q000633:toxicity; D010275:Parasympathetic Nervous System / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains / Q000502:physiology*; D012129:Respiratory Function Tests; D014132:Trachea / Q000187:drug effects
D000485:Allergens; D016085:Bronchoconstrictor Agents; D018678:Cholinergic Agents; D011189:Potassium Chloride; D002217:Carbachol; D010047:Ovalbumin