title: [The study of relations of cellular DNA content and proliferative activity to lymph node metastasis in gastric carcinoma].
abstract: OBJECTIVE
  To evaluate DNA/Cytokeratin(CK) multiparametric method in detection to the gastric cancer and to study the relations of cellular DNA content, S-phase fraction (SPF) and G2/M-phase fraction (G2/MPF) to lymph node (LN) metastasis.
  
  
  METHODS
  50 cases of fresh tumor samples, were examined by using single (DNA) and two-colored DNA/CK flow cytometry respectively.
  
  
  RESULTS
  The rate of aneuploid, the mean and the range of SPF and G2/MPF were higher in the multiparametric method than that in single one. In aneuploid carcinoma, the incidence of LN metastasis was significantly higher, the G2/MPF values with LN metastasis were higher than those without metastasis; values were higher in metastasis to n2 or n3 than that to n1. The SPF or G2/MPF values correlated with the number of LN metastasis, being significantly higher in metastasis to 5 LN than to 1-4 LN.
  
  
  CONCLUSIONS
  Multiparametric DNA/CK cytometric analysis is better than single DNA one; correlations between cellular DNA content, SPF, G2/MPF and the extent of LN matastasis exist.
authors.affiliation: First Hospital, China Medical University, Shenyang.
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007633:Keratins / Q000032:analysis; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000737:chemistry / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm; D007633:Keratins