title: [Relevance of infection with equine herpesvirus 1 (EHV-1) in a German thoroughbred stud: vaccination, abortion and diagnosis].
abstract: The aim of the present study was to clarify whether an EHV-1 induced abortion can be prognosticated by an increase of antibody titres, virus shedding and/or viraemia and whether the current abortion diagnostic is suitable. In this context the immune response post immunization and a possible reactivation were of great interest. For this purpose blood samples of 32 mares between the ages of 5-21 years were regularly investigated during a period of two years before and after vaccination and pregnancy. Neutralization tests, indirect immunofluorescence tests as well as PCR and virus isolation were used for EHV-1 diagnostics. It could be shown that the horses reacted individually to vaccination. In 14 cases a EHV-1-reactivation was suggested. An abortion prognosis was not possible even using serological, virological and molecular biological parameters. In addition, virus shedding and antibody titres were individual. An acute infection was detectable by a significant rise of antibodies and viraemia as well as virus shedding in the secretions. For the abortion diagnostics the antigen detection in combination with virus isolation and PCR from fetal lungs gave reliable results. In addition, the virological and serological investigation of the mare is recommendable. For prophylaxis we would advise a regular vaccination and strict hygiene.
authors.affiliation: Institut für Virologie, Fachbereich Veterinärmedizin, Freie Universität Berlin.
authors.forename: U
authors.initials: U
authors.lastname: Schröer
authors.forename: A
authors.initials: A
authors.lastname: Lange
authors.forename: P
authors.initials: P
authors.lastname: Glatzel
authors.forename: H
authors.initials: H
authors.lastname: Ludwig
authors.forename: K
authors.initials: K
authors.lastname: Borchers
mesh_terms: D000034:Abortion, Veterinary / Q000821:virology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005260:Female; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000145:classification / Q000302:isolation & purification; D006734:Horse Diseases / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control*; D006736:Horses; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D014765:Viral Vaccines; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines