Screening of non-Saccharomyces wine yeasts for the production of beta-D-xylosidase activity.
Fifty-four yeast strains belonging to the genera Candida, Dekkera, Hanseniaspora, Metschnikowia, Pichia, Rhodotorula, Schizosaccharomyces and Zygosaccharomyces, mainly isolated from grapes and wines, were screened for the production of beta-D-xylosidase activity. Beta-D-xylosidase activity was only detected in eight yeast strains belonging to the genera Hanseniaspora (H. osmophila and H. uvarum) and Pichia (P. anomala). Beta-D-xylosidase preparations active against p-nitrophenyl-beta-D-xyloside were characterised with respect to their optimal pH and temperature conditions. H. uvarum 11105 and 11107 and P. anomala 10320 beta-D-xylosidase preparations were active at pH and temperature ranges and at concentrations of glucose and ethanol usually found during winemaking processes.
Manzanares
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P
Departamento de Biotecnologia de Alimentos, Instituto de Agroquímica y Tecnologia de Alimentos (CSIC), Valencia, Spain. pmanz@iata.csic.es
Ramón
D
D
Querol
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A
D000431:Ethanol / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000032:analysis; D009596:Nitrophenols / Q000032:analysis; D010843:Pichia / Q000201:enzymology / Q000254:growth & development; D004718:Saccharomycetales / Q000201:enzymology / Q000254:growth & development; D013696:Temperature; D014920:Wine / Q000382:microbiology*; D014995:Xylosidases / Q000032:analysis / Q000096:biosynthesis*; D015003:Yeasts / Q000201:enzymology* / Q000254:growth & development
D009596:Nitrophenols; D000431:Ethanol; D006923:Hymecromone; D014995:Xylosidases; D005947:Glucose