Prenatal X-irradiation increases GFAP- and calbindin D28k-immunoreactivity in the medial subdivision of the nucleus of solitary tract in the rat.
Glial fibrillary acidic protein- (GFAP) and calbindin D28k-immunoreactivity (IR) were investigated in the medial subdivision of the nucleus of the solitary tract (mNST) of prenatally X-irradiated rats. Pregnant rats were exposed to a single whole-body X-irradiation on day 11 or 16 of gestation at a dose of 1. 3 Gy. The offspring were killed at 7-14 days of age for the immunohistochemical observations. Rat pups showed strong GFAP-IR at the level rostral to the obex when receiving X-rays on day 11 of gestation, with hypertrophy of astrocyte cell bodies and cytoplasmic processes, but weak GFAP-IR when receiving X-rays on day 16 of gestation. Calbindin D28k-IR was stronger in the animals receiving X-rays on day 11 or 16 of gestation compared to that in the control animals. In the present study, the increase of GFAP- and calbindin D28k-IR cells in the mNST might indicate that adaptative mechanisms are taking place to preserve integrated nervous system function and possibly, to provide neuroprotection.
Jacquin
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TD
Laboratoire de Biologie Fonctionnelle du Neurone, IAF, CNRS, Gif sur Yvette, France.
Xie
Q
Q
Miki
T
T
Satriotomo
I
I
Itoh
M
M
Takeuchi
Y
Y
D000222:Adaptation, Physiological / Q000528:radiation effects; D000818:Animals; D000906:Antibodies; D064092:Calbindin 1; D064026:Calbindins; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D009457:Neuroglia / Q000737:chemistry / Q000528:radiation effects; D009474:Neurons / Q000737:chemistry / Q000528:radiation effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D017552:Solitary Nucleus / Q000166:cytology* / Q000196:embryology / Q000528:radiation effects*
D000906:Antibodies; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D005904:Glial Fibrillary Acidic Protein; D064030:S100 Calcium Binding Protein G