Apoptosis in coxsackievirus B3-caused diseases: interaction between the capsid protein VP2 and the proapoptotic protein siva.
Coxsackievirus B3 (CVB3) is a common factor in human myocarditis. Apoptotic events are present in CVB3-induced disease, but it is unclear how CVB3 is involved in apoptosis and which viral proteins may induce the apoptotic pathway. In this report we demonstrate that the human and murine proapoptotic protein Siva specifically interact with the CVB3 capsid protein VP2. Furthermore, the transcription of Siva is strongly induced in tissue of CVB3-infected mice and is present in the same area which is positively stained for apoptosis, CD27, and CD70. It has been proposed that Siva is involved in the CD27/CD70-transduced apoptosis. Therefore, we suggest a molecular mechanism through which apoptotic events contributes to CVB3-caused pathogenesis.
Henke
A
A
Institute of Virology, Medical Center, Friedrich Schiller University Jena, Germany. i6hean@rz.uni-jena.de
Launhardt
H
H
Klement
K
K
Stelzner
A
A
Zell
R
R
Munder
T
T
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D053282:CD27 Ligand; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003384:Coxsackievirus Infections / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D029822:Enterovirus B, Human / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution; D014158:Transcription, Genetic; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000032:analysis
D015703:Antigens, CD; D051017:Apoptosis Regulatory Proteins; D053282:CD27 Ligand; C505771:CD70 protein, human; D036022:Capsid Proteins; D002352:Carrier Proteins; C505772:Cd70 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C106549:SIVA1 protein, human; C484569:Siva protein, mouse; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; C442554:VP2 protein, Echovirus