Minicircle kDNA microheterogeneity in Endotrypanum indicate diversity within this genus.
A comparison of kDNA restriction-endonuclease fragment patterns from strains representing selected Endotrypanum zymodemes was done by schizodeme analysis. As the degree of heterogeneity within mini-circles varied among species or strains of Endotrypanum, the fingerprint obtained with each of the restriction enzymes was unique for each of these parasites. The data have revealed that this trypanosomatid genus is much more complex than it was originally thought to be.
Franco
A M
AM
Departamento de Imunologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, 21045-900, Brasil.
Machado
G M
GM
Moreira
C F
CF
Grimaldi
G
G
D000818:Animals; D018105:DNA, Kinetoplast / Q000032:analysis*; D018740:Genetic Heterogeneity; D014644:Genetic Variation / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D007891:Leishmania / Q000145:classification / Q000201:enzymology / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D014351:Trypanosomatina / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D018105:DNA, Kinetoplast; D007527:Isoenzymes