Involvement of two plasmids in fenitrothion degradation by Burkholderia sp. strain NF100.
A bacterium capable of utilizing fenitrothion (O,O-dimethyl O-4-nitro-m-tolyl phosphorothioate) as a sole carbon source was isolated from fenitrothion-treated soil. This bacterium was characterized taxonomically as being a member of the genus Burkholderia and was designated strain NF100. NF100 first hydrolyzed an organophosphate bond of fenitrothion, forming 3-methyl-4-nitrophenol, which was further metabolized to methylhydroquinone. The ability to degrade fenitrothion was found to be encoded on two plasmids, pNF1 and pNF2.
Hayatsu
M
M
Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. ahmhaya@agr.shizuoka.ac.jp
Hirano
M
M
Tokuda
S
S
D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003227:Conjugation, Genetic; D018274:Electroporation / Q000379:methods; D005278:Fenitrothion / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D012988:Soil Microbiology
D007306:Insecticides; D005278:Fenitrothion