title: Liquid chromatography-electrospray time-of-flight mass spectrometry for on-line accurate mass determination and identification of cyclodepsipeptides in a crude extract of the fungus Metarrhizium anisopliae.
abstract: Electrospray ionisation time-of-flight mass spectrometry (ESI-TOF-MS) has been used for the detection and identification of destruxins (cyclodepsipeptides) in a crude extract of the fungus Metarrhizium anisopliae. HPLC-MS analyses were performed with a post-column addition of erythromycin as a reference compound (lock mass procedure). Seven destruxin derivatives could be identified on-line from their accurate masses (deviation from calculated values <5.5 ppm) through elemental composition calculations. As a highly sensitive and accurate method, ESI-TOF-MS proved to be very powerful for the analysis and dereplication of natural products in complex mixtures.
authors.affiliation: Boehringer Ingelheim Austria GmbH, Vienna.
authors.forename: O
authors.initials: O
authors.lastname: Potterat
authors.forename: K
authors.initials: K
authors.lastname: Wagner
authors.forename: H
authors.initials: H
authors.lastname: Haag
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D003904:Mitosporic Fungi / Q000737:chemistry*; D008970:Molecular Weight; D010456:Peptides, Cyclic / Q000737:chemistry*
chemical_list: D010456:Peptides, Cyclic