title: Integration of an immunosorbent assay system: analysis of secretory human immunoglobulin A on polystyrene beads in a microchip.
abstract: An immunosorbent assay system was integrated into a glass microchip. Polystyrene beads were introduced into a microchannel, and then human secretory immunoglobulin A (s-IgA) adsorbed on the bead surface was reacted with colloidal gold conjugated anti-s-IgA antibody and detected by a thermal lens microscope. The scale merits of liquid microspace on the molecular behavior remarkably contributed to reduced assay time. The integration cut the time necessary for the antigen-antibody reaction by 1/90, thus shortening the overall analysis time from 24 h to less than 1 h. Moreover, troublesome operations required for conventional immunosorbent assays could be replaced by simple operations.
authors.affiliation: Kanagawa Academy of Science and Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Tokeshi
authors.forename: T
authors.initials: T
authors.lastname: Odake
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Ooi
authors.forename: M
authors.initials: M
authors.lastname: Nakao
authors.forename: T
authors.initials: T
authors.lastname: Kitamori
mesh_terms: D000937:Antigen-Antibody Reactions; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000276:immunology; D008904:Miniaturization; D011137:Polystyrenes / Q000737:chemistry*; D012666:Semiconductors
chemical_list: D007071:Immunoglobulin A, Secretory; D011137:Polystyrenes