Actions and interactions of ADP, 5- HT, histamine and PAF on equine platelets.
Platelets are thought to play a role in equine diseases such as acute laminitis and verminous arteritis and may be involved in allergic disease. Mediators implicated in the pathophysiology of these conditions activate platelets and responses may be enhanced by interactions between mediators. The present study compared platelet aggregation, thromboxane production and release of radiolabelled [(3)H]5- HT in response to 5- HT, histamine, ADP and PAF alone and in combination in vitro.PAF caused concentration-related aggregation, [(3)H]5- HT release and thromboxane production. In contrast, ADP caused aggregation and 5- HT induced the release of [(3)H]5- HT with little effect on other platelet functions. Histamine had little or no effect on equine platelets. Addition of 5- HT (10 microM) prior to ADP significantly displaced the aggregation response curve to the left. The profile of responses to PAF, ADP and 5- HT suggests differential activation of intracellular signalling pathways regulating these events. The enhanced response to ADP in the presence of 5- HT may have implications in thromboembolic disease in the horse.
Bailey
S R
SR
Department of Veterinary Basic Sciences, The Royal Veterinary College, North Mymms, Hatfield, Hertfordshire, AL9 7TA.
Andrews
M J
MJ
Elliott
J
J
Cunningham
F
F
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D004347:Drug Interactions; D006632:Histamine / Q000494:pharmacology*; D006736:Horses / Q000097:blood / Q000502:physiology*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis
D010972:Platelet Activating Factor; D012701:Serotonin; D013929:Thromboxane B2; D000244:Adenosine Diphosphate; D006632:Histamine