title: Molecular epidemiology of an outbreak due to IRT-2 beta-lactamase-producing strains of Klebsiella pneumoniae in a geriatric department.
abstract: In February 1998, 195 patients in the geriatric department of a French hospital were screened for the presence of co-amoxiclav-resistant Klebsiella pneumoniae. Eleven co-amoxiclav-resistant isolates obtained all produced an identical IRT-2 beta-lactamase. These K. pneumoniae isolates were clonally related and harboured a c. 55 kb non-conjugative plasmid encoding a non-class-1 integron-located blaIRT-2 gene. This study underlines that geriatric departments may be a reservoir for antibiotic-resistant strains and that IRT beta-lactamase-producing strains may be nosocomial pathogens.
authors.affiliation: Service de Bactériologie-Virologie, Service de Gériatrie, Hôpital de Bicêtre, 78 rue du Général Leclerc, Assistance Publique/Hôpitaux de Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Girlich
authors.forename: A
authors.initials: A
authors.lastname: Karim
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: M H
authors.initials: MH
authors.lastname: Cavin
authors.forename: C
authors.initials: C
authors.lastname: Verny
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D019980:Amoxicillin-Potassium Clavulanate Combination / Q000494:pharmacology; D001426:Bacterial Proteins; D003428:Cross Infection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D018274:Electroporation; D005602:France; D005853:Geriatrics; D006748:Hospital Departments; D017403:In Situ Hybridization; D007525:Isoelectric Focusing; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D019980:Amoxicillin-Potassium Clavulanate Combination; C121000:TEM24 beta-lactamase; C104731:beta-lactamase IRT-2; D001618:beta-Lactamases