Simultaneous detection of endogenous lectins and their binding capacity at the single-cell level--a technical note.
Endogenous lectins are proteins/glycoproteins which selectively recognize distinct saccharide ligands and are different from immunoglobulins and carbohydrate-utilizing enzymes. Expression of these molecules can be detected immunohistochemically using nonblocking monoclonal antibodies (A1D6: anti-galectin-3, MR-15-2-2: anti-175 kD mannose receptor). Alternatively, biotinylated (neo)glycoconjugates which are recognized by a studied lectin can be employed as convenient probes to demonstrate specific binding of sugar epitopes by carbohydrate recognition domains (CRD) of endogenous lectins. In this study, we describe a new procedure for immunocytochemical visualization of the expression of endogenous lectins and glycochemical visualization of the reactivity of carbohydrate recognition domain(s), performed simultaneously at the single-cell level.
Fronková
V
V
Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
Holíková
Z
Z
Liu
F T
FT
Homolka
J
J
Rijken
D C
DC
André
S
S
Bovin
N V
NV
Smetana
K
K
Gabius
H J
HJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000032:analysis / Q000276:immunology; D019921:Biotinylation; D000939:Epitopes / Q000276:immunology; D037502:Galectin 3; D015726:Giant Cells / Q000737:chemistry*; D006001:Glycoconjugates / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000032:analysis* / Q000276:immunology; D037181:Lectins, C-Type; D008024:Ligands; D016676:Macrophages, Alveolar / Q000737:chemistry*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis / Q000276:immunology; D011975:Receptors, Mitogen / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000939:Epitopes; D037502:Galectin 3; D006001:Glycoconjugates; D037102:Lectins; D037181:Lectins, C-Type; D008024:Ligands; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D009844:Oligosaccharides; D011956:Receptors, Cell Surface; D011975:Receptors, Mitogen