A new HLA-A*02 allele, A*0234, detected by polymerase chain reaction using sequence-specific primers (PCR-SSP).
A novel HLA-A*02 allele, A*0234, was identified in a potential unrelated bone marrow donor typed by polymerase chain reaction using sequence-specific primers (PCR-SSP). Positive reactions obtained upon testing with PCR-SSP did not fit any known combination of alleles indicating the possible presence of a novel allele. Sequencing of clones from this individual revealed the presence of a novel allele, HLA-A*0234. The sequence of exons 2, 3 and part of exon 4 showed that A*0234 differed from A*02011 by a single nucleotide in exon 2 at position 282 (C to G). The nucleotide substitution results in an amino acid change at residue 70 (Histidine to Glutamine) in the alpha1 domain.
Moses
J H
JH
Tissue Typing Services, Australian Red Cross Blood Service, North East Region, Sydney, NSW. jmoses@arcbs.redcross.org.au
Greville
W D
WD
Downes
J
J
McClenahan
W
W
Kennedy
A
A
Dunckley
H
H
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D015789:HLA-A2 Antigen / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D018076:DNA, Complementary; D015789:HLA-A2 Antigen