title: Some evidences for the involvement of plasmid in diuron herbicide degradation.
abstract: Pseudomonas sp. strain Bk8 was isolated from field soil contaminated with different urea-herbicides. This strain is a plasmid (pBkB)-harbouring organism capable of complete degradation of diuron herbicide. Plasmid-cured strain Bk8M was obtained by treatment of Pseudomonas sp. Bk8 with Mitomycin C. This cured strain is capable of only partial degradation of diuron side chain and accumulated a phenolic compound in the medium during growing on diuron as a sole source of carbon and energy. Conjugation experiment was carried out using Bk8M as a recipient and Bk8 as a donor of pBk8 plasmid. The transconjugant was able to degrade a diuron without accumulation of phenolic compound. It was proposed that plasmid pBk8 is self-transmissible and involved in the degradation of diuron aromatic ring but it is not connected with the transformation of diuron into diuron phenol compound.
authors.affiliation: Department of Botany, Faculty of Science, Sohag, Egypt.
authors.forename: B A
authors.initials: BA
authors.lastname: El-Deeb
authors.forename: A M
authors.initials: AM
authors.lastname: Ali
authors.forename: K A
authors.initials: KA
authors.lastname: Ali
mesh_terms: D001673:Biodegradation, Environmental; D003227:Conjugation, Genetic; D003470:Culture Media; D004237:Diuron / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D006540:Herbicides / Q000378:metabolism*; D019216:Metals, Heavy / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006540:Herbicides; D019216:Metals, Heavy; D012989:Soil Pollutants; D004237:Diuron