Differential expression of four human dynamin-like protein variants in brain tumors.
Dynamin-like protein, a large GTP-binding protein, has recently been cloned, and studies have suggested that it is involved in the formation of coated vesicles. In this report, the differential expression of four human dynamin-like protein splice variants (HdynIV-wildtype [WT], -11, -26, and -37) from various brain tumors was identified by reverse transcription/polymerase chain reaction (RT-PCR). One novel variant (HdynIV-11), not described previously, was identified. The four alternatively spliced variants exhibited tissue specificity in normal tissues. The HdynIV-WT was strongly expressed in the brain, whereas HdynIV-37 was expressed in all tissues examined. Moreover, HdynIV-26 was dominant in the liver and apparently overexpressed in all astrocytomas and most meningiomas and adenomas. This report suggests that HdynIV-26 may cause aberrant protein trafficking and alter vesicle formation in brain tumors. Our results also suggest that dynamin-like protein is associated with various brain tumors and, more importantly, that aberrant expression of the HdynIV-26 variant may play a role in brain tumorigenesis.
Chen
C H
CH
Graduate Institute of Biochemistry, Kaohsiung Medical College, Taiwan, ROC.
Howng
S L
SL
Hwang
S L
SL
Chou
C K
CK
Liao
C H
CH
Hong
Y R
YR
D000236:Adenoma / Q000235:genetics / Q000473:pathology; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D034281:Dynamins; D005260:Female; D020558:GTP Phosphohydrolases; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics / Q000473:pathology; D008579:Meningioma / Q000235:genetics / Q000473:pathology; D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010911:Pituitary Neoplasms / Q000235:genetics / Q000473:pathology; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D008869:Microtubule-Associated Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D011994:Recombinant Proteins; D020558:GTP Phosphohydrolases; C110768:DNM1L protein, human; C519851:Dnm1l protein, rat; D034281:Dynamins