[Optimization of conditions for cultivation of the basidiomycete Coriolus hirsutus--producer of extracellular laccase].
The effects of various factors on the biosynthesis of extracellular laccase (EC 1.14.18.1) by the basidiomycete Coriolus hirsutus (Wulf.: Fr.) Quel. no. 072 during submerged cultivation were examined. Optimal parameters for cultivation in a fermenter of 10 l were determined: temperature, 28 degrees C; stirrer rotation speed, 160 rpm; and the inoculum volume, 15% of the working volume of the fermenter. The filtrate contained peroxidase, laccase, and phenol oxidase activities and displayed a high thermal stability.
Koroleva
O V
OV
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Stepanova
E V
EV
Gavrilova
V P
VP
Iakovleva
N S
NS
Landesman
E O
EO
Iaropolov
A I
AI
D001487:Basidiomycota / Q000166:cytology / Q000378:metabolism*; D003470:Culture Media; D042845:Laccase; D010088:Oxidoreductases / Q000096:biosynthesis*
D003470:Culture Media; D010088:Oxidoreductases; D042845:Laccase