Modulation of indoleamine 2,3-dioxygenase by interferon-gamma in human placental chorionic villi.
The effect of interferon-gamma on indoleamine 2,3-dioxygenase, a tryptophan catabolizing enzyme, was studied in cultured human placental chorionic villi. The activity of indoleamine 2,3-dioxygenase was markedly stimulated by interferon-gamma in a time- and concentration-dependent manner. Interferon-alpha and interferon-beta also showed a slight stimulatory effect on indoleamine 2,3-dioxygenase activity. The level of indoleamine 2,3-dioxygenase mRNA expression (determined by reverse transcription-polymerase chain reaction) was also enhanced by interferon-gamma. Interleukin-4 showed a dose-dependent inhibitory effect on interferon-gamma-induced stimulation of indoleamine 2,3-dioxygenase activity and mRNA expression.
Kudo
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Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.
Boyd
C A
CA
Sargent
I L
IL
Redman
C W
CW
D002824:Chorionic Villi / Q000187:drug effects / Q000201:enzymology*; D046508:Culture Techniques; D015870:Gene Expression; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism* / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D010920:Placenta / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger; D014366:Tryptophan Oxygenase / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D015847:Interleukin-4; D007371:Interferon-gamma; D014366:Tryptophan Oxygenase