title: Diagnosis of sporotrichosis in a donkey using direct fluorescein-labeled antibody testing.
abstract: A 4-year-old female donkey residing in an open field in Indiana was admitted for evaluation of facial lesions of 2 years duration. Cytologic and histologic examination of exudate and tissue from the lesions revealed a pyogranulomatous inflammatory reaction with numerous yeasts. Sporothrix schenckii was suspected to be the infectious agent; however, multiple culture attempts did not provide positive identification of the organism. Serologic examination supported infection with S. schenckii. A specific direct immunofluorescent antibody test performed on paraffin-embedded tissue sections confirmed the organism as S. schenckii. Clinical signs resolved after appropriate iodide therapy.
authors.affiliation: Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Irizarry-Rovira
authors.forename: L
authors.initials: L
authors.lastname: Kaufman
authors.forename: J A
authors.initials: JA
authors.lastname: Christian
authors.forename: S R
authors.initials: SR
authors.lastname: Reberg
authors.forename: S B
authors.initials: SB
authors.lastname: Adams
authors.forename: D B
authors.initials: DB
authors.lastname: DeNicola
authors.forename: W
authors.initials: W
authors.lastname: Rivers
authors.forename: J F
authors.initials: JF
authors.lastname: Hawkins
mesh_terms: D000818:Animals; D003937:Diagnosis, Differential; D018397:Equidae / Q000382:microbiology*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D013175:Sporothrix / Q000276:immunology; D013174:Sporotrichosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*