Multifunctional DNA conjugates for the in vitro selection of new catalysts.
DNA-substrate conjugates are required for the direct in vitro selection of novel DNA catalysts for reactions between two small reactants. Here we describe the introduction of all necessary features into ssDNA by a novel, multifunctional primer containing a flexible PEG spacer, an o-nitrobenzyl moiety allowing for selective photocleavage, and anthracene as a reactant, a fluorescence label and/or an immobilization tag. These components were checked individually and by a mock selection.
Hausch
F
F
Freie Universität Berlin, Fachbereich Biologie, Chemie und Pharmazie, Institut für Chemie, Thielallee 63, 14195 Berlin, Germany.
Jäschke
A
A
D000873:Anthracenes / Q000737:chemistry; D002384:Catalysis; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D009578:Nitrobenzenes / Q000737:chemistry; D010777:Photochemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D016133:Polymerase Chain Reaction
D000873:Anthracenes; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D009578:Nitrobenzenes; D011092:Polyethylene Glycols; C034020:anthracene