title: Analysis of cis-trans isomers and enantiomers of sertraline by cyclodextrin-modified micellar electrokinetic chromatography.
abstract: In this work development, optimization and validation of a cyclodextrin-modified micellar electrokinetic chromatography (CD-modified MEKC) method is proposed to resolve separation of the sertraline hydrochloride and synthesis-related substances. Sertraline hydrochloride, the cis-(1S,4S) enantiomer form, is used as an antidepressant therapeutic agent. A buffer concentration composed of 20 mM sodium borate, pH 9.0 with 50 mM sodium cholate, 15 mM sulfated beta-cyclodextrin and 5 mM hydroxypropyl-beta-cyclodextrin was found to be the most suitable background electrolyte. Quantitation of the impurities at levels of 0.1% in different samples of the bulk drug was determined. A comparison of the results with those obtained by HPLC methodology was also accomplished. The method proved appropriate for testing the purity of sertraline hydrochloride in bulk drug.
authors.affiliation: Faculty of Pharmacy and Biochemistry, Department of Analytical Chemistry and Physicochemistry, University of Buenos Aires, Junín, Argentina.
authors.forename: S E
authors.initials: SE
authors.lastname: Lucangioli
authors.forename: L G
authors.initials: LG
authors.lastname: Hermida
authors.forename: V P
authors.initials: VP
authors.lastname: Tripodi
authors.forename: V P
authors.initials: VP
authors.lastname: Rodríguez
authors.forename: E E
authors.initials: EE
authors.lastname: López
authors.forename: P D
authors.initials: PD
authors.lastname: Rouge
authors.forename: C N
authors.initials: CN
authors.lastname: Carducci
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D007536:Isomerism; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis* / Q000737:chemistry; D020280:Sertraline / Q000032:analysis* / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline