title: Nucleotide sequence and vaccinia expression of the nucleoprotein of a highly virulent, neurotropic strain of Newcastle disease virus.
abstract: The nucleoprotein (NP) of Newcastle disease virus (NDV) was selected to study the relative importance of an internal structural protein in the avian immune response. The NP gene of the virulent, neurotropic NDV Texas GB (TGB) strain was cloned and sequenced. Nucleotide sequence data for the NP gene allowed comparison of the deduced amino acid sequences for the NP genes of NDV-TGB and the avirulent duck isolate NDV-D26. These comparisons demonstrated an 89% nucleotide sequence homology and a 97% homology between the deduced amino acid sequences. The NDV-TGB NP expressed in recombinant vaccinia virus (rVAC) was electrophoretically and immunologically identical to the wild-type NDV-TGB. Although inoculation of chickens with the recombinant vaccinia virus expressing the NDV NP gene elicited anti-NDV antibodies in higher titers than in birds inoculated with live LaSota NDV, this strong anti-NDV response did not protect against lethal challenge with NDV-TGB.
authors.affiliation: North Carolina State University, College of Veterinary Medicine, Department of Microbiology, Pathology, and Parasitology, Raleigh 27606, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Ward
authors.forename: F J
authors.initials: FJ
authors.lastname: Fuller
authors.forename: Y
authors.initials: Y
authors.lastname: Mehrotra
authors.forename: E V
authors.initials: EV
authors.lastname: De Buysscher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002645:Chickens; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005347:Fibroblasts / Q000821:virology; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics / Q000472:pathogenicity*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014162:Transfection; D014611:Vaccination / Q000662:veterinary; D014616:Vaccinia virus / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*
chemical_list: D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014764:Viral Proteins; C409209:nucleoprotein, Newcastle disease virus