Differential effects of acute cold and footshock on the sleep of rats.
Several studies have shown that 1 h of immobilisation stress during the rat's active period results in rebound of paradoxical (PS) and slow wave sleep (SWS). Since the effects of stress on behaviour and physiological parameters vary according to the stimulus, the present study sought to examine the activation of the hypothalamic-pituitary-adrenal (HPA) axis and the sleep pattern of rats submitted to 1 h of footshock, immobilisation or cold, or 18 h of PS deprivation (PSD). Stress sessions began between 0900 and 0930 h. Immediately after the end of the stress session, or at the corresponding time for controls, animals were blood sampled for determination of ACTH and corticosterone (CORT) plasma levels. In Experiment 2, animals were implanted with electrodes for basal and post-stress polysomnographic recording (6 h long). The results showed that all stressors produced an activation of the HPA axis; however, footshock induced the largest ACTH levels, whereas cold resulted in the highest CORT levels. In regard to the sleep data, immobilisation and PSD led to a rebound of SWS (+16.87% and +9.37%, respectively) and PS (+42.45% and +55.25%, respectively). Immobilisation, however, induced an increased number of PS episodes, whereas PSD resulted in longer PS episodes. Cold stress produced an exclusive rebound of SWS (+14.23%) and footshock promoted sustained alertness during the animal's resting period (+47.18%). These results indicate that different stimuli altered the sleep pattern in a distinct manner; and these alterations might be related to the state of the HPA axis activation.
Palma
B D
BD
Department of Psychobiology, Universidade Federal de São Paulo, São Paulo, Brazil.
Suchecki
D
D
Tufik
S
S
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D003080:Cold Temperature; D003345:Corticosterone / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D012890:Sleep / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology*; D013312:Stress, Physiological / Q000097:blood*
D000324:Adrenocorticotropic Hormone; D003345:Corticosterone