title: Forensic identification of skeletal remains from members of Ernesto Che Guevara's guerrillas in Bolivia based on DNA typing.
abstract: We report the positive identification of several members of the guerrillas led by Ernesto "Che" Guevara on the 1960 s in Bolivia by means of DNA fingerprinting. Successful DNA typing of both short tandem repeat loci and the hypervariable region of the human mitochondrial DNA was achieved after extracting total DNA from bones obtained from two burial sites. Given the size of the Cuban database for the STR allele frequencies, a conservative approach was followed to estimate the statistical significance of the genetic evidence. The estimated probabilities of paternity for the two cases in which the paternity logic was applied were higher than 99%. One case was analyzed using mitochondrial DNA and could not be excluded from the identity proposed by the forensic anthropology team. A fourth case was identified by exclusion, on the basis of the positive identification of the other remains, the historical and other anthropological evidence.
authors.affiliation: Mammalian Cell Genetics Division, Center for Genetic, Engineering and Biotechnology, Havana, Cuba. orgsup@cigb.edu.cu
authors.forename: R
authors.initials: R
authors.lastname: Lleonart
authors.forename: E
authors.initials: E
authors.lastname: Riego
authors.forename: M V
authors.initials: MV
authors.lastname: Saínz de la Peña
authors.forename: K
authors.initials: K
authors.lastname: Bacallao
authors.forename: F
authors.initials: F
authors.lastname: Amaro
authors.forename: M
authors.initials: M
authors.lastname: Santiesteban
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: H
authors.initials: H
authors.lastname: Currenti
authors.forename: A
authors.initials: A
authors.lastname: Puentes
authors.forename: F
authors.initials: F
authors.lastname: Rolo
authors.forename: L
authors.initials: L
authors.lastname: Herrera
authors.forename: J
authors.initials: J
authors.lastname: de la Fuente
mesh_terms: D000483:Alleles; D001838:Bolivia; D001842:Bone and Bones; D004272:DNA, Mitochondrial / Q000235:genetics*; D005554:Forensic Medicine; D005787:Gene Frequency; D006801:Humans; D016133:Polymerase Chain Reaction; D014857:Warfare
chemical_list: D004272:DNA, Mitochondrial