Preservation of 5'-end integrity of a potyvirus genomic RNA is not dependent on template specificity.
Full-length in vitro transcripts of plum pox potyvirus (PPV) genomic RNA with mutations altering the number of 5'-terminal adenosine residues were able to infect Nicotiana clevelandii plants, whereas a mutant with a substitution of adenosine in position 2 by guanosine failed to infect. The genomic 5' end was template-independently repaired during in vivo RNA synthesis producing wild-type viral progeny. Putative models of replication initiation are discussed.
Simón-Buela
L
L
Centro Nacional de Biotecnología, CSIC, Campus UAM, Madrid, 28049, Spain.
Osaba
L
L
García
J A
JA
López-Moya
J J
JJ
D016296:Mutagenesis; D010935:Plant Diseases; D010947:Plants, Toxic; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000502:physiology*; D017423:Sequence Analysis, RNA; D013329:Structure-Activity Relationship; D013698:Templates, Genetic; D014026:Nicotiana; D014779:Virus Replication
D012367:RNA, Viral