title: Development of lentiviral vectors for gene therapy for human diseases.
abstract: Retroviral vectors derived from murine retroviruses are being used in several clinical gene therapy trials. Recently, progress has been made in the development of vectors based on the lentivirus genus of retroviruses, which ironically includes a major human pathogen, human immunodeficiency virus (HIV). As these vector systems for clinical gene transfer are developed, it is important to understand the rationale behind their design and development. This article reviews the fundamental features of retrovirus replication and of the elements necessary for development of a retroviral vector system, and it discusses why vector systems based on HIV or other lentiviruses have the potential to become important tools in clinical gene therapy. (Blood. 2000;95:2499-2504)
authors.affiliation: Division of Hematology/Oncology and the Departments of Medicine and Biology, University of California at San Diego, La Jolla, CA 92093-0665, USA. gbuchschacher@ucsd.edu
authors.forename: G L
authors.initials: GL
authors.lastname: Buchschacher
authors.forename: F
authors.initials: F
authors.lastname: Wong-Staal
mesh_terms: D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus