Coping with replication 'train wrecks' in Escherichia coli using Pol V, Pol II and RecA proteins.
DNA replication machineries tend to stall when confronted with damaged DNA template sites, causing the biochemical equivalent of a major 'train wreck'. A newly discovered bacterial DNA polymerase, Escherichia coli Pol V, acting in conjunction with the RecA protein, can exchange places with the stalled replicative Pol III core and catalyse 'error-prone' translesion synthesis. In contrast to Pol V-catalysed 'brute-force, sloppier copying', another SOS-induced DNA polymerase, Pol II, plays a pivotal role in an 'error-free', replication-restart DNA repair pathway and probably involves RecA-mediated homologous recombination.
Goodman
M F
MF
Dept of Biological Sciences and Chemistry, University of Southern California, University Park, Los Angeles, CA 90089-1340, USA. mgoodman@mizar.usc.edu
D002384:Catalysis; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D016296:Mutagenesis; D013014:SOS Response, Genetics
D029968:Escherichia coli Proteins; D004257:DNA Polymerase II; C120926:DNA polymerase V, E coli; D004259:DNA-Directed DNA Polymerase