Effect of temperature on the separation of soybean 11 S and 7 S protein fractions during bipolar membrane electroacidification.
The purpose of this study was to evaluate the effect of temperature (10 and 27 degrees C) on the efficiency of bipolar membrane electroacidification (BMEA) to fractionate soybean proteins. BMEA is a technology derived from electrodialysis, based on the isoelectric precipitation of proteins. It appears that temperature has a significant effect on the selective precipitation of the soybean protein fractions, mainly 11 S and 7 S, during BMEA. At 27 degrees C, the precipitation profile of the four protein fractions is situated in a pH range from 6.6 to 4.4, with no possibility of separating any of theses fractions. However, at 10 degrees C, the 11 S globulin precipitates at a higher pH than at 27 degrees C, pH 6.7 vs 5.9, allowing the fractionation of 11 S from the other fractions. Using electroacidification it is possible to obtain a precipitate solution enriched in the 11 S fraction (71.8% of 11 S and 10.8% of 7 S) and a supernatant solution enriched in the 7 S fraction (46.6% of 7 S and 4.6% of 11S).
Bazinet
L
L
Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600 Casavant Blvd. West, St. Hyacinthe (Québec), Canada J2S 8E3.
Ippersiel
D
D
Labrecque
R
R
Lamarche
F
F
D005591:Chemical Fractionation / Q000379:methods; D004563:Electrochemistry / Q000379:methods*; D008567:Membranes, Artificial; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D012995:Solubility; D013025:Glycine max / Q000737:chemistry*; D013696:Temperature
D008567:Membranes, Artificial; D010940:Plant Proteins