title: RPP13 is a simple locus in Arabidopsis thaliana for alleles that specify downy mildew resistance to different avirulence determinants in Peronospora parasitica.
abstract: Disease resistance (R) genes are found in plants as either simple (single allelic series) loci, or more frequently as complex loci of tandemly repeated genes. These different loci are likely to be under similar evolutionary forces from pathogens, but the contrast between them suggests important differences in mechanisms associated with DNA structure and recombination that generate and maintain R gene diversity. The RPP13 locus in Arabidopsis represents an important paradigm for studying the evolution of an R gene at a simple locus. The RPP13 allele from the accession Nd-1, designated RPP13-Nd, confers resistance to five different isolates of the biotrophic oomycete, Peronospora parasitica (causal agent of downy mildew), and encodes an NBS-LRR type R protein with a putative amino-terminal leucine zipper. The RPP13-Rld allele, cloned from the accession Rld-2, encodes a different specificity. Comparison of three RPP13 alleles revealed a high rate of amino acid divergence within the LRR domain, less than 80% identity overall, compared to the remainder of the protein (> 95% identity). We also found evidence for positive selection in the LRR domain for amino acid diversification outside the core conserved beta-strand/beta-turn motif, suggesting that more of the LRR structure is available for interaction with target molecules than has previously been reported for other R gene products. Furthermore, an amino acid sequence (LLRVLDL) identical in an LRR among RPP13 alleles is conserved in other LZ NBS-LRR type R proteins, suggesting functional significance.
authors.affiliation: Horticulture Research International, Wellesbourne, Warwick, UK.
authors.forename: P D
authors.initials: PD
authors.lastname: Bittner-Eddy
authors.forename: I R
authors.initials: IR
authors.lastname: Crute
authors.forename: E B
authors.initials: EB
authors.lastname: Holub
authors.forename: J L
authors.initials: JL
authors.lastname: Beynon
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins; D002874:Chromosome Mapping; D008969:Molecular Sequence Data; D009868:Oomycetes / Q000302:isolation & purification / Q000472:pathogenicity*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; C408688:RPP13 protein, Arabidopsis