title: Crystallization and preliminary X-ray diffraction studies of phospholipase D from Streptomyces sp.
abstract: Crystals of purified phospholipase D (E.C. 3.1.4.4) from Streptomyces sp. strain PMF have been grown under two different crystallization conditions using vapour diffusion. Both conditions gave monoclinic crystals in space group P2(1). The unit-cell parameters were a = 57.28, b = 57.42, c = 68.70 A, beta = 93.17 degrees. The crystals diffract at 110 K to a resolution beyond 1.4 A using synchrotron radiation.
authors.affiliation: Department of Chemistry, Faculty of Science, University of Tromso, Norway.
authors.forename: I
authors.initials: I
authors.lastname: Leiros
authors.forename: E
authors.initials: E
authors.lastname: Hough
authors.forename: P
authors.initials: P
authors.lastname: D'Arrigo
authors.forename: G
authors.initials: G
authors.lastname: Carrea
authors.forename: G
authors.initials: G
authors.lastname: Pedrocchi-Fantoni
authors.forename: F
authors.initials: F
authors.lastname: Secundo
authors.forename: S
authors.initials: S
authors.lastname: Servi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010739:Phospholipase D / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D013302:Streptomyces / Q000201:enzymology*
chemical_list: D010739:Phospholipase D