Biliary excretion of bile acids and organic anions in zone 1- and zone 3-injured rats.
OBJECTIVE
Hepatocytes in zone 1 of the hepatic lobule play a role in the uptake and biliary excretion of bile acids and organic anions under physiological conditions, and those in zone 3 may play a role only with a high-dose load. To further elucidate the role of hepatic zonation on the handling of bile acids and organic anions, biliary excretion of these compounds was studied in zone 1- and zone 3-injured rats.


METHODS
Biliary excretion of bile acids and organic anions was studied in zone 1- and zone 3-injured rats, caused by allyl alcohol and bromobenzene, respectively.


RESULTS
Biliary excretion of a tracer dose of taurocholate was unchanged in the injury in both zones, but that of leukotriene C4 was decreased in zone 1 injury. The excretory maximum of taurocholate was decreased with zone 1 and the zone 3 injuries. Biliary excretion of deoxycholate metabolites was decreased in zone 3 injury, although the profile of metabolites in the bile was unchanged. Sulfobromophthalein excretion was decreased in zone 1 injury, but unchanged in zone 3 injury.


CONCLUSIONS
These findings indicate that zone 1 is very important for biliary excretion of both organic anions and bile acids. In contrast, zone 3 is considered not to have a role in biliary excretion of organic anions, but to play a role in the excretion of bile acids.
Aiso
M
M
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Takikawa
H
H
Yamanaka
M
M
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001652:Bile Ducts / Q000378:metabolism*; D001969:Bromobenzenes / Q000633:toxicity; D017997:Leukotriene C4 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020005:Propanols / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013448:Sulfobromophthalein / Q000378:metabolism; D013656:Taurocholic Acid / Q000378:metabolism*
D001969:Bromobenzenes; D020005:Propanols; D013448:Sulfobromophthalein; D017997:Leukotriene C4; C006463:allyl alcohol; D013656:Taurocholic Acid; C032036:bromobenzene; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase