Methylene blue reverts multidrug resistance: sensitivity of multidrug resistant cells to this dye and its photodynamic action.
Photodynamic action has been advocated as an alternative treatment of tumors but the most common used dyes, hematoporphyrin derivatives, are substrate for P-glycoprotein. This study investigated the MDR-reverting properties of methylene blue (MB) and compared the sensitivity to its photodynamic action (PDA) in five cell lines that either express or do not express the MDR phenotype. MB was able to revert the MDR phenotype and there was no difference in sensitivity to MB-PDA between MDR and non-MDR cells, suggesting that MB has the advantage of being used simultaneously as a MDR reverser and a photodynamic agent.
Trindade
G S
GS
Departamento de Ciências Fisiológicas, FURG, Rio Grande, Brazil.
Farias
S L
SL
Rumjanek
V M
VM
Capella
M A
MA
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D020014:K562 Cells; D007668:Kidney / Q000166:cytology / Q000187:drug effects; D004915:Leukemia, Erythroblastic, Acute / Q000188:drug therapy / Q000473:pathology; D008751:Methylene Blue / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D010641:Phenotype; D010778:Photochemotherapy; D014750:Vincristine / Q000494:pharmacology
D014750:Vincristine; D008751:Methylene Blue