[Effects of icariin on the differentiation of HL-60 cells].
OBJECTIVE
In order to search for new tumor differentiation inducer, the effects and mechanism of Icariin were studied.


METHODS
Icariin is an monomer purified from Epimedium Koreanum Nakai. The differentiation of induction effects of Icariin were observed on the human promyelocytic leukemic cell (HL-60) by NBT reduction test, 125I-cAMP and 125I-cGMP double antibody radioimmune methods, scanning electronic microscopy technique.


RESULTS
After treating HL-60 cells with Icariin at concentration 0.1 g/L, reduction of NBT and cAMP/cGMP ratio were increased. There were many rugosities and ball-like processes on the cell surface.


CONCLUSIONS
Icariin had the effects of induction of differentiation on HL-60 cells, the mechanism might be related to elevating the cAMP/cGMP ratio.
Zhao
Y
Y
Shandong Provincial Qianfoshan Hospital, Jinan.
Cui
Z
Z
Zhang
L
L
D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D018922:HL-60 Cells / Q000648:ultrastructure; D006801:Humans
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D000242:Cyclic AMP; D006152:Cyclic GMP; C056599:icariin