[The action of catecholamine-synthesis inhibitors and of spiperone on sea urchin and mouse embryos].
We studied the effects of three inhibitors of catecholamine synthesis on the development of sea urchins Sphaerechinus granularis and Paracentrotus lividus. These drugs affected the early embryogenesis, which was expressed in inhibition of the cleavage divisions, appearance of abnormal embryos, and developmental arrest. The addition of arachidonic acid amide and dopamine to the incubation medium weakened the effects of the inhibitors. Spiperone induced developmental defects in preimplantation mouse embryos and sea urchin embryos. Arachidonic acid amide with dopamine exerted a protective effect against spiperone when introduced to sea urchin embryos at the blastula or late gastrula stages, rather than after fertilization. In murine embryos, this amide induced developmental defects and arrest itself and its effect was reversible. Possible mechanisms underlying the effects of these drugs are discussed.
Markova
L N
LN
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Sakharova
N Iu
NIu
Bezuglov
V V
VV
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects*; D002395:Catecholamines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004298:Dopamine / Q000031:analogs & derivatives / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008750:Methyldopa / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D007470:Monoiodotyrosine / Q000494:pharmacology*; D012617:Sea Urchins / Q000187:drug effects / Q000196:embryology; D013134:Spiperone / Q000494:pharmacology*; D019805:alpha-Methyltyrosine / Q000494:pharmacology*
D002395:Catecholamines; D016895:Culture Media, Serum-Free; D018492:Dopamine Antagonists; D004791:Enzyme Inhibitors; D013134:Spiperone; D008750:Methyldopa; D019805:alpha-Methyltyrosine; D007470:Monoiodotyrosine; D004298:Dopamine