Inhibition of LPS-induced NO production and NF-kappaB activation by a sesquiterpene from Saussurea lappa.
To elucidate the molecular mechanisms for the suppression of LPS-induced nitric oxide (NO) production by a dehydrocostus lactone (DL) from Saussurea lappa, we examined the preventive effect of this compound on NF-kappaB activation in LPS-treated RAW 264.7 macrophages and U937 human monocytic cells. The results suggest that the suppression of NO production is mediated by the inhibitory action on the i-NOS gene expression through the inactivation of NF-kappaB and this sesquiterpene lactone can act as a pharmacological inhibitor of the NF-kappaB activation.
Jin
M
M
Research Institutes of Pharmaceutical Sciences, Seoul, Korea.
Lee
H J
HJ
Ryu
J H
JH
Chung
K S
KS
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012717:Sesquiterpenes / Q000494:pharmacology*; D020298:U937 Cells
D008070:Lipopolysaccharides; D016328:NF-kappa B; D012717:Sesquiterpenes; D009569:Nitric Oxide; D004247:DNA; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse