Evaluating GA733-2 mRNA as a marker for the detection of micrometastatic breast cancer in peripheral blood and bone marrow.
The GA733-2 gene encodes the epithelial glycoprotein 40, a homophilic cell-cell adhesion molecule, which is expressed on the surface of epithelial cells and associated with a variety of carcinomas, e.g. breast, colorectal and lung carcinomas. To test if it could serve as a tumor marker, we have analysed the expression of GA733-2 in bone marrow (BM) and peripheral blood (PB) from healthy donors, and of patients with haematological malignancies or breast cancer using reverse transcriptase-polymerase chain reaction (RT-PCR). The GA733-2 nested PCR was positive in 100% (8/8) of BM and 40% (16/40) of PB from healthy donors, in 100% (33/33) of BM from patients with breast cancer who had no evidence of distant metastases and also in BM and PB from patients with haematological malignancies. GA733-2 mRNA is not specific as a marker for the detection of breast cancer cells in BM and PB.
Zhong
X Y
XY
Department of Obstetrics and Gynaecology, University Basel.
Kaul
S
S
Eichler
A
A
Bastert
G
G
D000595:Amino Acid Sequence / Q000235:genetics; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000097:blood / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D001853:Bone Marrow / Q000473:pathology; D019046:Bone Marrow Neoplasms / Q000175:diagnosis* / Q000556:secondary*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000097:blood / Q000235:genetics; D018929:Cell Culture Techniques; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D019337:Hematologic Neoplasms / Q000097:blood / Q000473:pathology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule