title: An adipocyte-derived plasma protein, adiponectin, adheres to injured vascular walls.
abstract: Adipose tissue secretes a variety of proteins into the bloodstream. We have previously reported a novel cDNA, apM1 (adipose most abundant gene transcript 1), which is specifically and abundantly expressed in adipose tissue [1]. Primary structure analysis predicted that the apM1 gene product possesses significant homology to collagens VIII, X and complement factor C1q, and we named it adiponectin. In the current study, we analyzed characteristics of adiponectin in vitro and in vivo. Adiponectin protein was proved to be secreted into the medium when the cDNA was transfected to COS cells. Anti-adiponectin cross-reactivities were abundantly detected in the human plasma. In solid-phase binding assays, adiponectin specifically bound to collagen types I, III and V, which are present in vascular intima. Immunohistochemical analysis revealed that adiponectin was detected in the walls of the catheter-injured vessels but not in the intact vascular walls. These data suggest that adiponectin is a plasma protein produced by adipose tissue and accumulates in vascular walls when the endothelial barrier is injured.
authors.affiliation: Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Arita
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: M
authors.initials: M
authors.lastname: Muraguchi
authors.forename: N
authors.initials: N
authors.lastname: Ouchi
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Igura
authors.forename: Y
authors.initials: Y
authors.lastname: Inui
authors.forename: S
authors.initials: S
authors.lastname: Kihara
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: J
authors.initials: J
authors.lastname: Miyagawa
authors.forename: T
authors.initials: T
authors.lastname: Funahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuzawa
mesh_terms: D017667:Adipocytes / Q000737:chemistry* / Q000378:metabolism; D052242:Adiponectin; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D019556:COS Cells; D020212:Carotid Artery Injuries / Q000378:metabolism*; D003094:Collagen / Q000032:analysis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D008297:Male; D011485:Protein Binding / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D052242:Adiponectin; D001798:Blood Proteins; D016326:Extracellular Matrix Proteins; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D011506:Proteins; D003094:Collagen