title: Differences in rat rbc cytosol induced after in vivo phenylhydrazine treatment.
abstract: Analysis of rat red blood cell (RBC) cytosol protein fraction after in vivo phenylhydrazine treatment revealed increased amounts of 68-kDa protein. This protein is present in trace amounts in normal rat RBC cytosol. We also present data that 68-kDa protein from RBC cytosol has an identical isoelectric point, partial proteolytic map and immunological determinants as a protein of the same molecular mass from rat exosomes. These data indicate that 68-kDa protein is normally present in rat RBC cytosol, is exported via exosomes during reticulocyte maturation, and is increased in induced haemolytic anaemia.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
authors.forename: A
authors.initials: A
authors.lastname: Djurković
authors.forename: J
authors.initials: J
authors.lastname: Zarić
authors.forename: M
authors.initials: M
authors.lastname: Lusić
authors.forename: V
authors.initials: V
authors.lastname: Glisin
authors.forename: Z
authors.initials: Z
authors.lastname: Popović
mesh_terms: D000743:Anemia, Hemolytic / Q000139:chemically induced / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism; D015153:Blotting, Western; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D004912:Erythrocytes / Q000187:drug effects*; D005089:Exocytosis / Q000187:drug effects; D010449:Peptide Mapping; D010659:Phenylhydrazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D001798:Blood Proteins; D010659:Phenylhydrazines; C030299:phenylhydrazine; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin