title: Isolation and characterization of an equine foamy virus.
abstract: Foamy viruses (FVs) are complex retroviruses which have been isolated from different animal species including nonhuman primates, cattle, and cats. Here, we report the isolation and characterization of a new FV isolated from blood samples of horses. Similar to other FVs, the equine foamy virus (EFV) exhibits a highly characteristic ultrastructure and induces syncytium formation and subsequent cell lysis on a large number of cell lines. Molecular cloning of EFV reveals that the general organization is that of other known FVs, whereas sequence similarity with its bovine FV counterpart is only 40%. Interestingly, EFV buds exclusively from the plasma membrane and not from the endoplasmic reticulum (ER), as previously shown for other FVs. The absence of the ER retrieval dilysine motif in EFV Env is likely responsible for this unexpected sorting pathway.
authors.affiliation: CNRS UPR9051, Université Paris 7, Hôpital Saint-Louis, 75475 Paris Cedex 10, France.
authors.forename: J
authors.initials: J
authors.lastname: Tobaly-Tapiero
authors.forename: P
authors.initials: P
authors.lastname: Bittoun
authors.forename: M
authors.initials: M
authors.lastname: Neves
authors.forename: M C
authors.initials: MC
authors.lastname: Guillemin
authors.forename: C H
authors.initials: CH
authors.lastname: Lecellier
authors.forename: F
authors.initials: F
authors.lastname: Puvion-Dutilleul
authors.forename: B
authors.initials: B
authors.lastname: Gicquel
authors.forename: S
authors.initials: S
authors.lastname: Zientara
authors.forename: M L
authors.initials: ML
authors.lastname: Giron
authors.forename: H
authors.initials: H
authors.lastname: de Thé
authors.forename: A
authors.initials: A
authors.lastname: Saïb
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D019556:COS Cells; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis; D015752:Genes, env; D015750:Genes, gag; D015751:Genes, pol; D006734:Horse Diseases / Q000097:blood / Q000276:immunology / Q000821:virology*; D006736:Horses; D006801:Humans; D008969:Molecular Sequence Data; D011533:Proviruses / Q000235:genetics; D011817:Rabbits; D012192:Retroviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016092:Spumavirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D020079:Terminal Repeat Sequences; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral