Integration of an immunosorbent assay system: analysis of secretory human immunoglobulin A on polystyrene beads in a microchip.
An immunosorbent assay system was integrated into a glass microchip. Polystyrene beads were introduced into a microchannel, and then human secretory immunoglobulin A (s-IgA) adsorbed on the bead surface was reacted with colloidal gold conjugated anti-s-IgA antibody and detected by a thermal lens microscope. The scale merits of liquid microspace on the molecular behavior remarkably contributed to reduced assay time. The integration cut the time necessary for the antigen-antibody reaction by 1/90, thus shortening the overall analysis time from 24 h to less than 1 h. Moreover, troublesome operations required for conventional immunosorbent assays could be replaced by simple operations.
Sato
K
K
Kanagawa Academy of Science and Technology, Japan.
Tokeshi
M
M
Odake
T
T
Kimura
H
H
Ooi
T
T
Nakao
M
M
Kitamori
T
T
D000937:Antigen-Antibody Reactions; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000276:immunology; D008904:Miniaturization; D011137:Polystyrenes / Q000737:chemistry*; D012666:Semiconductors
D007071:Immunoglobulin A, Secretory; D011137:Polystyrenes