Decrease of adenosine A-1 receptor gene expression in cerebral cortex of aged rats.
Changes of adenosine A-1 receptor (A1-AR) gene expression in aging were investigated in cerebral cortex using the rat aged from 2 months (adult) to 24 months (aged). Quantification of A1-AR protein level by immunoblotting analysis showed an age-related decrease of A1-AR in cerebral cortex of Wistar rats. Compared to the preparations from 2-month-old animals, the levels of A1-AR in the 6-, 12-, and 24-month-old rats were reduced by 14.3+/-5.2, 32.5+/-4.5 and 28.2+/-5.7%, respectively. Similar decrease of mRNA level in A1-AR was also obtained using Northern blotting analysis. Two representative spots of mRNA, a 3.4-kb transcript and a 5.6-kb transcript, were observed in X-ray film from cerebral cortex of rat hybridized with rat A1-AR cDNA probe. Compared to the 2 month-old rats, levels of the 5.6-kb transcript were decreased by 17.9+/-2.5, 27.4+/-3.2 and 23.1+/-2.1% in the 6-, 12- and 24-month-old rats, respectively. These results indicated an age-related decrease of A1-AR in cerebral cortex of the rat that seems responsible for the change of response to adenosine.
Cheng
J T
JT
Department of Pharmacology College of Medicine, National Cheng Kung University, Tainan City, Taiwan, China. jtcheng@mail.ncku.edu.tw
Liu
I M
IM
Juang
S W
SW
Jou
S B
SB
D000375:Aging / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry / Q000235:genetics; D002540:Cerebral Cortex / Q000378:metabolism* / Q000502:physiology; D015536:Down-Regulation / Q000235:genetics*; D005786:Gene Expression Regulation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018047:Receptors, Purinergic P1 / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification
D018047:Receptors, Purinergic P1