Microvessel count, proliferating cell nuclear antigen and Ki-67 indices in gastric adenocarcinoma.
The aim of the present study was to immunohistochemically investigate the prognostic value of neovascularization (expressed as microvessel count-MVC) and tumor cell proliferation (expressed as PCNA labeling index PLI and Ki-67 labeling index KLI) in gastric adenocarcinoma. Correlations with clinicopathologic features were also evaluated. Tumor specimens from 74 patients diagnosed as gastric adenocarcinoma were included in this study. Formalin fixed, paraffin embedded tissue sections stained immunohistochemically with F-VIII, PC10 and MIB-1 monoclonal antibodies. By ocular grid subdivided into 100 areas, number of microvessels and PC10, MIB-1 positive and negative cells were counted at x400 magnification. Chi-square test, Kaplan-Meier method and cox regression analysis were used for statistical analysis. The results showed that, MVC and PLI had a significant correlation with invasion and lymph node metastasis. The prognosis was significantly worse in patients with high MVC (>14 ) and with high PLI (>49%). However any relationship was not observed between KLI (38%) and clinicopathologic parameters, so KLI failed to predict the prognosis. Cox model showed that, MVC and PLI were independent prognostic variables. Ki-67 labeling index in gastric carcinomas has no prognostic relevance. However, the evaluation of microvessel count and proliferating cell nuclear antigen index in gastric carcinomas could be reliable indicators of prognosis.
Elpek
G O
GO
Akdeniz University, Medical School, Department of Pathology Týp Fakültesi, Patoloji ABD, Yeni Týp, Dekanlýk, Antalya, 07070, Turkey. elpek@hipokrat.med.akdeniz.edu.tr
Gelen
T
T
Aksoy
N H
NH
Karpuzoglu
T
T
Keles
N
N
D000230:Adenocarcinoma / Q000098:blood supply / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis*; D034961:Antigens, Nuclear; D015415:Biomarkers; D014408:Biomarkers, Tumor / Q000032:analysis*; D002455:Cell Division; D004730:Endothelium, Vascular / Q000737:chemistry; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000032:analysis*; D015993:Life Tables; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic; D009687:Nuclear Proteins / Q000032:analysis; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D016016:Proportional Hazards Models; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000098:blood supply / Q000737:chemistry / Q000401:mortality / Q000473:pathology*; D016019:Survival Analysis; D014421:Turkey / Q000453:epidemiology; D014841:von Willebrand Factor / Q000032:analysis
D000951:Antigens, Neoplasm; D034961:Antigens, Nuclear; D015415:Biomarkers; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D014841:von Willebrand Factor