Targeted insertion of a lacZ reporter gene into the mouse Cer1 locus reveals complex and dynamic expression during embryogenesis.
The mouse Cer1 (mCer1, Cer-l, Cerr1) gene encodes one member of a family of cytokines structurally and functionally related to the Xenopus head-inducing factor, Cerberus (xCer). We generated a mouse line in which the Cer1 gene was inactivated by replacing the first coding exon with a lacZ reporter gene. Mice homozygous for this allele (Cer1(lacZ)) showed no apparent perturbation of embryogenesis or later development. However, the lacZ reporter revealed a number of hitherto uncharacterised sites of Cer1 expression in late fetal and adult tissues. Preliminary analysis suggests that Cer1 is not essential for their morphogenesis, differentiation, or homeostasis.
Stanley
E G
EG
Biben
C
C
Allison
J
J
Hartley
L
L
Wicks
I P
IP
Campbell
I K
IK
McKinley
M
M
Barnett
L
L
Koentgen
F
F
Robb
L
L
Harvey
R P
RP
D000818:Animals; D029681:Arabidopsis Proteins; D005314:Embryonic and Fetal Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D010940:Plant Proteins / Q000235:genetics*
D029681:Arabidopsis Proteins; C101547:CER protein, Arabidopsis; D010940:Plant Proteins