CD10 expression in cultured human osteoblast-like cells.
Morphological features, bone nodule formation and alkaline phosphatase activity are currently used to identify osteoblasts. CD10 (cALLa antigen) is a glycoprotein with endopeptidase activity and it is present on the surface of many cell types. We have studied the expression of CD10 in osteoblast-like cells by immunocytochemistry and flow cytometry in order to identify other markers of the osteoblast lineage. We isolated osteoblast-like cells from specimens obtained in the course of oral surgery. Expression of the cALLa antigen (CD10) may also be an indicator of the osteoblast phenotype.
Reyes-Botella
C
C
Departamento Estomatología, Instituto de Neurociencias, Universidad de Granada, Spain.
Montes
M J
MJ
Abadía-Molina
A C
AC
Vallecillo-Capilla
M F
MF
Ruiz
C
C
D000469:Alkaline Phosphatase / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D019070:Cell Lineage; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D015260:Neprilysin / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism*; D015675:Osteocalcin / Q000032:analysis; D010641:Phenotype
D015415:Biomarkers; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D015260:Neprilysin