title: Ruminant relaxin in the pregnant one-humped camel (Camelus dromedarius).
abstract: We have determined the cDNA sequence of preprorelaxin in the pregnant one-humped camel by employing reverse transcription- and rapid amplification of cDNA ends-polymerase chain reaction. Camel preprorelaxin consisted of 600 base pairs (bp) encoding a protein of 199 amino acids (aa) with a signal peptide of 25 aa (75 bp), a B domain of 28 aa (84 bp), a C domain of 121 aa (366 bp), and an A domain of 24 aa (72 bp). The N terminus of the C domain of camel prorelaxin contained the unique proline-rich repetitive sequence (-RPAP)(3)-(-K/RPAL-)(2), and within the B domain the classical -GRELVR- receptor binding motif was found. Camel preprorelaxin showed highest homology with porcine (74.6%) and equine (65.4%) relaxin. The ovary and the uteroplacental unit were a dual source of relaxin in the pregnant dromedary. Within the ovary, weak expression of relaxin was detected in large luteal cells of the mature corpus luteum. In the ovarian follicles, immunoreactive relaxin, but not relaxin mRNA, was detected in the granulosa and theca interna cell layer. Beginning at around Day 93 of gestation and coinciding with increasing interdigitation of the fetal villus with the underlying maternal endometrium, uterine luminal epithelial cells in the uteroplacental tissue expressed relaxin. Weak expression of immunoreactive relaxin, but not relaxin mRNA, was observed in villous trophoblast cells. Pseudostratified trophoblast cells at the base of the placental villi and multinucleate giant cells did not express relaxin.
authors.affiliation: Department of Anatomy and Cell Biology, Martin Luther University, Faculty of Medicine, D-06097 Halle (Saale), Germany. sabine.hombach-klonisch@medizin.uni-halle.de
authors.forename: S
authors.initials: S
authors.lastname: Hombach-Klonisch
authors.forename: M
authors.initials: M
authors.lastname: Abd-Elnaeim
authors.forename: J A
authors.initials: JA
authors.lastname: Skidmore
authors.forename: R
authors.initials: R
authors.lastname: Leiser
authors.forename: B
authors.initials: B
authors.lastname: Fischer
authors.forename: T
authors.initials: T
authors.lastname: Klonisch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000502:physiology*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004076:Digoxigenin / Q000378:metabolism; D005260:Female; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D012065:Relaxin / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012065:Relaxin; D004247:DNA; D004076:Digoxigenin