Suppressive effects of dominant negative ras mutant N116Y on transformed phenotypes of human bladder cancer cells.
To investigate the suppressive effect of dominant negative H-ras mutant N116Y on transformed phenotypes, we established two N116Y ras mutant stable transfectant clones (C5, C13) of human bladder cancer cell line, UMUC-2. These N116Y ras mutant transfectants, especially the C5 cells, showed a dramatic change of cellular morphology and significantly reduced growth in soft agar compared to their control. Furthermore, phosphorylation of the Jun NH2-terminal kinase (JNK) was significantly decreased in these transfectants compared to the control. These results suggest that the N116Y-induced suppression of transformed phenotypes in UMUC-2 cells is associated with inhibition of JNK phosphorylation.
Watanabe
T
T
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Shinohara
N
N
Sazawa
A
A
Kobayashi
Y
Y
Ogiso
Y
Y
Kimura
T
T
Takiguchi
M
M
Yasuda
J
J
Hashimoto
A
A
Koyanagi
T
T
Kuzumaki
N
N
D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002999:Clone Cells; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D005799:Genes, Dominant; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*