Study of the in vitro maturation of mouse oocytes induced by microinjection of maturation promoting factor (MPF).
OBJECTIVE
Maturation promoting factor (MPF) acts at the resumption of meiosis and nonspecifically throughout the animal species. There exists a considerable body of literature on MPF, but little work has been done to study the induction of maturation of mammalian oocytes by microinjection of extracted MPF.


METHODS
Immature (GV-stage) mouse oocytes were microinjected MPF extracted from matured Xenopus eggs in the presence of dbcAMP.


RESULTS
The rate of germinal vesicle, breakdown (GVBD) induced at 24 hr after MPF injection was significantly higher (90.5%) than that of the control (2.2%), which was injected with HTF medium containing dbcAMP (P < 0.0001). The rate of extrusion of the first polar body at 24 hr after MPF injection was significantly higher (84.1%) than that of the same control (1.1%) (P < 0.0001).


CONCLUSIONS
From these results, it is concluded that the maturation of mammalian oocytes can be induced by the microinjection of MPF extracted from other species.
Nakano
H
H
First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan.
Kubo
H
H
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D008297:Male; D016200:Maturation-Promoting Factor / Q000302:isolation & purification / Q000494:pharmacology*; D000090204:Mesothelin; D051379:Mice; D008813:Mice, Inbred ICR; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D014982:Xenopus laevis
C000715528:Msln protein, mouse; D000242:Cyclic AMP; D016200:Maturation-Promoting Factor; D000090204:Mesothelin