title: Increased levels of GALbeta1-4GLCNACalpha2-6 sialyltransferase pretransplant predict delayed graft function in kidney transplant recipients.
abstract: BACKGROUND
  Galbeta1-4GlcNAcalpha2-6 sialyltransferase (ST6GalI) is an acute phase reactant whose release from cells can be induced by proinflammatory cytokines. Because patients with chronic renal failure have high circulating levels of proinflammatory cytokines, we hypothesized that patients on the renal transplant waiting list would have high circulating levels of ST6GalI, which might adversely affect post-transplant events.
  
  
  METHODS
  Levels of ST6GalI were measured in the serum of 70 patients immediately before renal transplant; these were correlated with posttransplant events, such as delayed graft function and rejection.
  
  
  RESULTS
  The mean serum level of ST6GalI was significantly higher in the patients (3162+/-97 U) than in 19 controls (2569 +/- 125 U; P<0.003). Patients who required dialysis posttransplant for treatment of delayed graft function (n=20) had significantly higher levels of ST6GalI pretransplant (3735+/-228 U) than patients (n=50) who did not require dialysis (2933+/-83 U; P<0.0001). In a multivariate analysis the ST6GalI level and cold ischemic time were found to be independent risk factors for the development of delayed graft function.
  
  
  CONCLUSIONS
  ST6GalI levels are high in renal failure patients awaiting a renal transplant and may be a risk factor for the development of delayed graft function. The assessment and perhaps modulation of a potential transplant recipient's ST6GalI systemic level may be beneficial.
authors.affiliation: Department of Chemistry, University of Manitoba, Winnipeg, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Thorne-Tjomsland
authors.forename: T
authors.initials: T
authors.lastname: Hosfield
authors.forename: J C
authors.initials: JC
authors.lastname: Jamieson
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Nickerson
authors.forename: J C
authors.initials: JC
authors.lastname: Gough
authors.forename: D N
authors.initials: DN
authors.lastname: Rush
authors.forename: J R
authors.initials: JR
authors.lastname: Jeffery
authors.forename: R M
authors.initials: RM
authors.lastname: McKenna
mesh_terms: D015925:Cryopreservation; D005260:Female; D006801:Humans; D007668:Kidney / Q000503:physiopathology*; D016030:Kidney Transplantation; D008297:Male; D015999:Multivariate Analysis; D011182:Postoperative Care; D011379:Prognosis; D017582:Renal Replacement Therapy; D012307:Risk Factors; D012799:Sialyltransferases / Q000097:blood*; D013997:Time Factors; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase