[A new potential promoter region of the human connexin 26 gene].
OBJECTIVE
To explain regulatory mechanism of the human connexin 26(Cx26) gene.


METHODS
A DNase-1 hypersensitive 1.6 kb fragment upstream of the 5'-terminus of the Cx26 gene was sequenced and assayed with CAT reporter system.


RESULTS
The Cx26-1.6 kb sequence serves as a powerful promoter.


CONCLUSIONS
The Cx26-1.6 kb fragment contains two GT boxes (centering at-6158 and -6213 bp), and a TATA-less TTAAAA box (-6237/-6232 bp) which is another promoter region of the human Cx26.
Peng
B
B
Cancer Research Institute, Hunan Medical University, Changsha, Hunan, 410078 P.R. China.
Zhang
X
X
Zhou
M
M
Zeng
Z
Z
Li
G
G
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D000072259:Connexin 26; D017630:Connexins / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
D017630:Connexins; C000607042:GJB2 protein, human; D000072259:Connexin 26; D015500:Chloramphenicol O-Acetyltransferase