Induction of a novel cellular homolog of interleukin-10, AK155, by transformation of T lymphocytes with herpesvirus saimiri.
Although herpesvirus saimiri-transformed T lymphocytes retain multiple normal T-cell functions, only a few changes have been described. By subtractive hybridization, we have isolated a novel cellular gene, ak155, a sequence homolog of the interleukin-10 gene. Specifically herpesvirus saimiri-transformed T cells overexpress ak155 and secrete the protein into the supernatant. In other T-cell lines and in native peripheral blood cells, but not in B cells, ak155 is transcribed at low levels. AK155 forms homodimers similarly to interleukin-10. As a lymphokine, AK155 may contribute to the transformed phenotype of human T cells after infection by herpesvirus saimiri.
Knappe
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Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91054 Erlangen, Germany.
Hör
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S
Wittmann
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Fickenscher
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H
D000595:Amino Acid Sequence; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D006567:Herpesvirus 2, Saimiriine / Q000502:physiology*; D006801:Humans; D016753:Interleukin-10 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007378:Interleukins; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D014158:Transcription, Genetic
C406767:IL26 protein, human; D007378:Interleukins; D016753:Interleukin-10