Frequency and clinicopathology associations of K-ras mutations in colorectal cancer in a northeast Mexican population.
Activation of the ras family gene has been implicated in colorectal tumorigenesis, K-ras being the most frequently altered gene. The frequency of K-ras codon 12, 13 and 61 point mutations in patients with colorectal neoplasias was examined. We employed a polymerase chain reaction-restriction fragment length polymorphism assay and single-strand conformational polymorphism to detect mutations. We found that point mutations at codons 12 and 13 were present in 53% and 39% of the tumors, respectively, but none at codon 61. These results agree with previous reports. Point mutations were more frequent in adenomas than in carcinomas, with villous adenomas presenting a higher incidence of mutations than other adenomas. The association between clinical and histopathological parameters was investigated. Our study is the beginning of a new research line in molecular epidemiology of colorectal cancer and is the first to be carried out in one part of the Mexican population.
Martinez-Garza
S G
SG
Departamento de Bioquimica, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico.
Núñez-Salazar
A
A
Calderon-Garcidueñas
A L
AL
Bosques-Padilla
F J
FJ
Niderhauser-García
A
A
Barrera-Saldaña
H A
HA
D000230:Adenocarcinoma / Q000453:epidemiology* / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D003113:Colonoscopy; D015179:Colorectal Neoplasms / Q000453:epidemiology* / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D015994:Incidence; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D017354:Point Mutation; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm