title: Gene-specific DNA repair of pyrimidine dimers does not decline during cellular aging in vitro.
abstract: A large number of studies have demonstrated that various kinds of DNA damage accumulate during aging and one of the causes for this could be a decrease in DNA repair capacity. However, the level of total genomic repair has not been strongly correlated with aging. DNA repair of certain kinds of damage is known to be closely connected to the transcription process; thus, we chose to investigate the level of gene-specific repair of UV-induced damage using in vitro aging of human diploid skin fibroblasts and trabecular osteoblasts as model systems for aging. We find that the total genomic repair is not significantly affected during cellular aging of cultures of both human skin fibroblasts and trabecular osteoblasts. Gene-specific repair was analyzed during cellular aging in the dihydrofolate reductase housekeeping gene, the p53 tumor suppressor gene, and the inactive region X(754). There was no clear difference in the capacity of young and old cells to repair UV-induced pyrimidine dimers in any of the analyzed genes. Thus, in vitro senescent cells can sustain the ability to repair externally induced damage.
authors.affiliation: Danish Centre for Molecular Gerontology, University of Aarhus, Aarhus C, DK-8000, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Christiansen
authors.forename: T
authors.initials: T
authors.lastname: Stevnsner
authors.forename: V A
authors.initials: VA
authors.lastname: Bohr
authors.forename: B F
authors.initials: BF
authors.lastname: Clark
authors.forename: S I
authors.initials: SI
authors.lastname: Rattan
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology / Q000528:radiation effects; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000528:radiation effects; D012150:Polymorphism, Restriction Fragment Length; D011740:Pyrimidine Dimers / Q000378:metabolism*; D015183:Restriction Mapping; D012867:Skin / Q000166:cytology*; D016615:Telomere / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; D013762:Tetrahydrofolate Dehydrogenase