The chaperonin-related protein Tcm62p ensures mitochondrial gene expression under heat stress.
Tcm62p, distantly related to chaperonins, is required for the assembly of succinate dehydrogenase in mitochondria of Saccharomyces cerevisiae and was proposed to exert chaperone activity. We demonstrate here crucial functions of Tcm62p under heat stress. It ensures mitochondrial gene expression at elevated temperatures and prevents heat-aggregation of the ribosomal subunit Var1p. Similar to chaperonins, Tcm62p forms a high molecular mass protein complex of approximately 850 kDa in the mitochondrial matrix space. These results suggest a more general chaperone function of Tcm62p in mitochondria.
Klanner
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Adolf-Butenandt-Institut für Physiologische Chemie der Universität München, Goethestr. 33, 80336, Munich, Germany.
Neupert
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Langer
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D019069:Cell Respiration / Q000235:genetics; D018834:Chaperonin 60 / Q000378:metabolism; D018833:Chaperonins / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000235:genetics*; D019286:Endopeptidase K / Q000378:metabolism; D005656:Fungal Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D007106:Immune Sera; D008565:Membrane Proteins; D008928:Mitochondria / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D011233:Precipitin Tests; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics; D012269:Ribosomal Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013816:Thermodynamics
D018834:Chaperonin 60; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D007106:Immune Sera; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; C116028:TCM62 protein, S cerevisiae; C026383:VAR1 protein, S cerevisiae; D019286:Endopeptidase K; D018833:Chaperonins