Isolation and characterisation of wheat cDNA clones encoding PR4 proteins.
Two cDNA clones encoding the previously characterised PR4 proteins wheatwin 1 and wheatwin2 from wheat (Triticum aestivum cv. S. Pastore) have been identified and named wPR4a and wPR4b, respectively. The clones have been isolated by screening a cDNA library with a specific cDNA probe obtained by RT-PCR. The wPR4a and wPR4b cDNAs contain open reading frames of 441 and 447 bp that encode for wheatwin1 and wheatwin2, respectively.
Caruso
C
C
Dipartimento di Agrobiologia e Agrochimica, Universitá della Tuscia, Italy. caruso@unitus.it
Bertini
L
L
Tucci
M
M
Caporale
C
C
Leonardi
L
L
Saccardo
F
F
Bressan
R A
RA
Veronese
P
P
Buonocore
V
V
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006026:Glycoside Hydrolases / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; D006026:Glycoside Hydrolases