title: Antibody responses of goldfish (Carassius auratus L.) to DNA-immunisation at different temperatures and feeding levels.
abstract: DNA vaccines which work in winter are needed for fish. At 18 weeks after intramuscular injection of a plasmid containing lacZ goldfish at 15 degrees C had five-fold more antibody to betagalactosidase than those at 25 degrees C or those at </=9 degrees C. Expression persisted for 1 week at 25 degrees C and for 4 weeks at 15 degrees C. Expression was highest after 18 weeks at </=9 degrees C and was correlated to antibody titre in this group. Goldfish on low or high rations (0.33 or 3% body weight daily) made the same amount of antibody whilst losing 20% or gaining 50% of their body-weight over 2 months at 22 degrees C.
authors.affiliation: Department of Pathology, The Royal Veterinary College, Royal College Street, London, UK. prussell@rvc.ac.uk
authors.forename: P H
authors.initials: PH
authors.lastname: Russell
authors.forename: T
authors.initials: T
authors.lastname: Kanellos
authors.forename: M
authors.initials: M
authors.lastname: Negrou
authors.forename: A A
authors.initials: AA
authors.lastname: Ambali
mesh_terms: D000818:Animals; D000917:Antibody Formation; D004435:Eating; D015870:Gene Expression; D006054:Goldfish / Q000276:immunology*; D007273:Injections, Intramuscular; D007763:Lac Operon; D018485:Muscle Fibers, Skeletal / Q000201:enzymology; D010957:Plasmids / Q000235:genetics; D012621:Seasons; D013696:Temperature; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology
chemical_list: D019444:Vaccines, DNA; D001616:beta-Galactosidase