title: [Low expression of nm23-H1 mRNA and its protein in breast cancer].
abstract: OBJECTIVE
  To explore the expression of nm23-H1 mRNA and its protein in breast cancer and its correlation to the biologic behavior.
  
  
  METHODS
  RT-PCR and immunohistochemical method were employed to detect the expression of nm23-H1 mRNA and its protein in 58 breast cancer tissues.
  
  
  RESULTS
  Low level expression of nm23-H1 mRNA and protein was shown in 44.8% and 48.2% of examined cases respectively. The low expression of nm23-H1 mRNA was correlated, but not always parallel to the protein expression. The low level expression of nm23-H1 mRNA or the protein was significantly associated with lymphatic metastasis of the cancer.
  
  
  CONCLUSIONS
  Examination of nm23-H1 expression may be of significance in evaluating metastatic potentiality and prognosis of breast cancer.
authors.affiliation: Department of General Surgery, Dalian Central Hospital, Dalian, Liaoning, 116033 P.R.China.
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Zhuang
authors.forename: Y
authors.initials: Y
authors.lastname: Bai
authors.forename: J
authors.initials: J
authors.lastname: Zhang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis / Q000235:genetics*; D054778:NM23 Nucleoside Diphosphate Kinases; D009362:Neoplasm Metastasis; D009701:Nucleoside-Diphosphate Kinase; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*
chemical_list: D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins