title: Effect of hemostatics used during operations for digestive organ on cancer cells present in the peritoneal cavity.
abstract: We investigated effects of hemostatics used during operations for digestive organ on cancer cells present in the peritoneal cavity using BALB/c mice inoculated with Meth A tumor cells (fibrosarcoma) intraperitoneally (i.p.) and C3H/He mice inoculated with MH134 tumor cell (hepatic cell carcinoma) i.p. Microfibrillar collagen hemostat (Avitene) or fibrinogen preparation (Beriplast P) did not affect survivals of those tumor-bearing mice. Gelatin sponge (Spongel)prolonged survivals of MH134 tumor-bearing mice. Liquid form gelatin used instead of Spongel displayed in vitro antitumor effect on MH134 tumor cells at the concentration of 15 mg/ml. Radioactive sodium chromate-labeled MH134 and Meth A tumor cells were not lysed when they were incubated with 15 mg/ml of liquid form gelatin for 24 hours. On the other hand, the tritium thymidine (3H-TdR) uptake by MH134 or RL male 1 tumor cells was suppressed when they were incubated with 15 mg/ml of liquid form gelatin for 24 hours. Proliferation of Meth A tumor cells were not affected by the treatment. Effect of liquid form gelatin on phytohemagglutinin (PHA)-stimulated spleen cells as a benign counter-part of RL male 1 tumor cells (T cell lymphoma) was examined. Liquid form gelatin (15 mg/ml) did not suppress 3H-TdR uptake by PHA-stimulated spleen cells.
authors.affiliation: First Department of Surgery, Aichi Medical University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Naruse
mesh_terms: D000818:Animals; D003094:Collagen / Q000627:therapeutic use*; D013505:Digestive System Surgical Procedures; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D005354:Fibrosarcoma / Q000188:drug therapy / Q000473:pathology; D005780:Gelatin / Q000627:therapeutic use*; D006490:Hemostatics / Q000627:therapeutic use*; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000188:drug therapy / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010529:Peritoneal Cavity
chemical_list: D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D005780:Gelatin; D003094:Collagen