Cell surface presentation of recombinant (poly-) peptides including functional T-cell epitopes by the AIDA autotransporter system.
For the efficient surface presentation and release of virulence factors especially pathogenic Gram-negative bacteria have developed several distinct secretion mechanisms. An increasing number of pathogens in various species employs a mechanism denoted the 'autotransporter' pathway. This pathway is characterised by an outer membrane translocator module representing the C-terminal domain of the transported protein itself. An intriguing potential application of such systems involves the transport and surface expression of recombinant proteins or peptides, like e.g. the presentation of antigens for the generation of live oral vectors as vaccine carriers. Here we report on the incorporation of heterologous (poly-) peptides in permissive sites of the translocator module of the adhesin-involved-in-diffuse-adherence (AIDA) autotransporter system. We demonstrate the presentation of the B subunit of the heat labile enterotoxin of Escherichia coli (LTB) as well as of functional T-cell epitopes of Yersinia enterocolitica heat-shock protein 60 (Y-hsp60) on the surface of E. coli.
Konieczny
M P
MP
Institut für Infektiologie-Zentrum für Molekularbiologie der Entzündung (ZMBE), Westfälische Wilhelms-Universität Münster, Von-Esmarch-Str. 56, 48149, Münster, Germany.
Suhr
M
M
Noll
A
A
Autenrieth
I B
IB
Alexander Schmidt
M
M
D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D018834:Chaperonin 60 / Q000276:immunology / Q000378:metabolism; D003001:Cloning, Molecular; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D015008:Yersinia enterocolitica / Q000276:immunology / Q000378:metabolism
C075559:AIDA-I protein, E coli; D018830:Adhesins, Escherichia coli; D000954:Antigens, Surface; D001427:Bacterial Toxins; D002352:Carrier Proteins; D018834:Chaperonin 60; D004768:Enterotoxins; D018984:Epitopes, T-Lymphocyte; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; C027538:heat-labile enterotoxin, E coli