title: A single C-terminal peptide segment mediates both membrane association and localization of lysyl hydroxylase in the endoplasmic reticulum.
abstract: Hydroxylation of lysyl residues is crucial for the unique glycosylation pattern found in collagens and for the mechanical strength of fully assembled extracellular collagen fibers. Hydroxylation is catalyzed in the lumen of the endoplasmic reticulum (ER) by a specific enzyme, lysyl hydroxylase (LH). The absence of the known ER-specific retrieval motifs in its primary structure and its association with the ER membranes in vivo have suggested that the enzyme is localized in the ER via a novel retention/retrieval mechanism. We have identified here a 40-amino acid C-terminal peptide segment of LH that is able to convert cathepsin D, normally a soluble lysosomal protease, into a membrane-associated protein. The same segment also markedly slows down the transport of the reporter protein from the ER into post-ER compartments, as assessed by our pulse-chase experiments. The retardation efficiency mediated by this C-terminal peptide segment is comparable with that of the intact LH but lower than that of the KDEL receptor-based retrieval mechanism. Within this 40-amino acid segment, the first 25 amino acids appear to be the most crucial ones in terms of membrane association and ER localization, because the last 15 C-terminal amino acids did not possess substantial retardation activity alone. Our findings thus define a short peptide segment very close to the extreme C terminus of LH as the only necessary determinant both for its membrane association and localization in the ER.
authors.affiliation: Department of Anatomy and Cell Biology, University of Oulu, PL 5000, FIN-90401 Oulu, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Suokas
authors.forename: R
authors.initials: R
authors.lastname: Myllyla
authors.forename: S
authors.initials: S
authors.lastname: Kellokumpu
mesh_terms: D000818:Animals; D019556:COS Cells; D002402:Cathepsin D / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D006056:Golgi Apparatus / Q000201:enzymology; D006801:Humans; D007425:Intracellular Membranes / Q000201:enzymology; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018000:Receptors, Peptide / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection
chemical_list: C064459:KDEL receptor; D010446:Peptide Fragments; D018000:Receptors, Peptide; D011994:Recombinant Proteins; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase; D002402:Cathepsin D