Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems.
The origin of the Cre recombinase gene is bacteriophage P1, and thus the codon usages are different from in mammals. In order to adapt this codon usage for mammals, we synthesized a "mammalian Cre recombinase gene" and examined its expression in Chinese hamster ovarian tumor (CHO) cells. Significant increases in protein production as well as mRNA levels were observed. When the recombination efficiency was compared using CHO cell transfectants having a cDNA containing loxP sites, the "mammalian Cre recombinase gene" recombined the loxP sites much more efficiently than the wild-type Cre recombinase gene.
Koresawa
Y
Y
Division of Organ Transplantation, Biomedical Research Center, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Osaka 565-0871, Japan.
Miyagawa
S
S
Ikawa
M
M
Matsunami
K
K
Yamada
M
M
Shirakura
R
R
Okabe
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D016466:CHO Cells; D003062:Codon; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D013997:Time Factors; D014162:Transfection; D014764:Viral Proteins
D003062:Codon; D018076:DNA, Complementary; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases