title: A tetrodotoxin-producing Vibrio strain, LM-1, from the puffer fish Fugu vermicularis radiatus.
abstract: Identification of tetrodotoxin (TTX) and its derivatives produced from a Vibrio strain in the intestine of the puffer fish Fugu vermicularis radiatus was performed by thin-layer chromatography, electrophoresis, high-performance liquid chromatography, and gas chromatography-mass spectrometry, together with a mouse bioassay for toxicity. It was demonstrated that the isolated bacterium produced TTX, 4-epi-TTX, and anhTTX during cultivation, suggesting that Vibrio strains are responsible for the toxification of the puffer fish.
authors.affiliation: Department of Diagnosis, Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, Kyung-Ju 780-714, Korea.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lee
authors.forename: D Y
authors.initials: DY
authors.lastname: Jeong
authors.forename: W S
authors.initials: WS
authors.lastname: Kim
authors.forename: H D
authors.initials: HD
authors.lastname: Kim
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: W W
authors.initials: WW
authors.lastname: Park
authors.forename: Y H
authors.initials: YH
authors.lastname: Park
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: D S
authors.initials: DS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001681:Biological Assay; D003470:Culture Media; D005400:Fishes, Poisonous / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology; D051379:Mice; D013779:Tetrodotoxin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D014733:Vibrio / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013779:Tetrodotoxin