[Tumor samples as a tool for large-scale monitoring of antigen-specific lymphocytes in gastric and colorectal neoplasms].
The characterization of tumor-associated antigens has enabled to direct the host immune response towards the autologous tumor through appropriate loading and presentation of the antigen. In vivo conditions that generate large numbers of tumor antigens would be an important step in vaccine strategies. In this study we have therefore tested the ability of freshly isolated gastric and colorectal cancer cells to induce a specific anti-tumor response in autologous T lymphocytes. Because dendritic cells (DC) are critically involved in both initiating and boosting host immune responses, they have been used to present apoptotic bodies generated by irradiated tumor cells. Results show that these native antigens stimulate T cytotoxic response against tumor, but not peritumor normal tissues. Induction of IFN-gamma secreting cell activity, which is a standard readout in current cancer vaccine protocols, was also demonstrated by Elispot single-cells assay. These data show the antigenicity of gastric and colorectal tumor cells and open new perspectives in immunotherapy.
Matera
L
L
Dipartimento di Medicina Interna, Università degli Studi di Torino.
Galetto
A
A
Contarini
M
M
Sapino
A
A
Consalvo
M
M
Cassoni
P
P
Moro
F
F
Mussa
B
B
Mussa
A
A
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology*; D000368:Aged; D000920:Antibody-Dependent Cell Cytotoxicity; D015179:Colorectal Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured