Functional assessment of precursors from murine bone marrow suggests a sequence of early B lineage differentiation events.
Most lineage marker-negative (Lin-)TdT+ cells from murine marrow lack CD34 but display c-kit at low density as well as IL-7Ralpha and Flk-2/Flt-3 receptors. Single cells with these characteristics generated CD45RA+CD19- as well as CD19+ lymphocytes in culture. CD45RA+CD19- marrow cells were resolved into three nonoverlapping subsets. One subset, lacking DX5 and Ly-6C antigens, yielded CD19+ cells in culture. Further analysis demonstrated CD24 on most Lin-TdT+ cells and all CD45R+CD19-DX5-Ly-6C- cells. Mac-1/CD11b was absent from these two subsets of B lineage precursors, while IL-7Ralpha was retained during subsequent differentiation to a CD19+ and stromal cell-independent stage. These findings contrast with previous descriptions of B lymphocyte precursors and suggest a sequence of early differentiation events.
Tudor
K S
KS
Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Payne
K J
KJ
Yamashita
Y
Y
Kincade
P W
PW
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018941:Antigens, CD19 / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D051927:CD24 Antigen; D002454:Cell Differentiation; D019070:Cell Lineage; D004253:DNA Nucleotidylexotransferase / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D017493:Leukocyte Common Antigens / Q000378:metabolism; D019891:Leukopoiesis / Q000502:physiology; D051917:Leukosialin; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D020395:Receptors, Interleukin-7 / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism
D015703:Antigens, CD; D018941:Antigens, CD19; D015415:Biomarkers; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D051917:Leukosialin; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D020395:Receptors, Interleukin-7; D012795:Sialoglycoproteins; C497863:Spn protein, mouse; D004253:DNA Nucleotidylexotransferase; D017493:Leukocyte Common Antigens