Salivary amylase activity of the phlebotomine sand fly, Lutzomyia longipalpis.
Both male and female adult stages of the sand fly Lutzomyia longipalpis have detectable amylase activity in their salivary glands, as indicated by formation of p-nitrophenyl-alpha-D-maltoside from p-nitrophenyl-alpha-D-octoside and by hydrolysis of 4-nitrophenyl-alpha-D-maltoheptaoside-4,6,-O-ethylidene. No salivary alpha-glucosidase was detected. Amylase activity was also found in the crop and midgut of female flies, although in a smaller amount. Salivary amylase is significantly reduced from the salivary glands immediately after a blood meal, as is the case with salivary alpha-glucosidases in mosquitoes. Presence of salivary gland amylase in these sand flies, and absence of salivary alpha-glucosidase, indicates that in nature these insects may have a significant intake of carbohydrates in the form of starch, as suggested by their plant-feeding behavior, previously demonstrated by Schlein and Warburg (Schlein, Y., Warburg, A., 1986. Phytophagy and the feeding cycle of Phlebotomus papatasi (Diptera: Psychodidae) under experimental conditions. Journal of Medical Entomology 23, 11-15), and Alexander and Usma (Alexander, B., Usma, M.C., 1994. Potential sources of sugar for the phlebotomine sandfly Lutzomyia youngi (Diptera: Psychodidae) in a Columbia coffee plantation. Ann. Trop. Med. Parasitol. 88, 543-549).
Ribeiro
J M
JM
Section of Medical Entomology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, MSC-0425, NIH, Bethesda 20892-0425, USA. jribeiro@nih.gov
Rowton
E D
ED
Charlab
R
R
D000818:Animals; D005260:Female; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D011576:Psychodidae / Q000201:enzymology*; D012469:Salivary Glands / Q000201:enzymology*; D013379:Substrate Specificity; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
D000516:alpha-Amylases