title: Identification and characterization of cell lines with a defect in a post-adsorption stage of Sendai virus-mediated membrane fusion.
abstract: In the early stage of infection, Sendai virus delivers its genome into the cytoplasm by fusing the viral envelope with the cell membrane. Although the adsorption of virus particles to cell surface receptors has been characterized in detail, the ensuing complex process that leads to the fusion between the lipid bilayers remains mostly obscure. In the present study, we identified and characterized cell lines with a defect in the Sendai virus-mediated membrane fusion, using fusion-mediated delivery of fragment A of diphtheria toxin as an index. These cells, persistently infected with the temperature-sensitive variant Sendai virus, had primary viral receptors indistinguishable in number and affinity from those of parental susceptible cells. However, they proved to be thoroughly defective in the Sendai virus-mediated membrane fusion. We also found that viral HN protein expressed in the defective cells was responsible for the interference with membrane fusion. These results suggested the presence of a previously uncharacterized, HN-dependent intermediate stage in the Sendai virus-mediated membrane fusion.
authors.affiliation: Department of Neurovirology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, 565-0871, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Eguchi
authors.forename: T
authors.initials: T
authors.lastname: Kondoh
authors.forename: H
authors.initials: H
authors.lastname: Kosaka
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Momota
authors.forename: A
authors.initials: A
authors.lastname: Masago
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Taira
authors.forename: A
authors.initials: A
authors.lastname: Ishii-Watabe
authors.forename: J
authors.initials: J
authors.lastname: Okabe
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.forename: N
authors.initials: N
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Taki
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
authors.forename: M
authors.initials: M
authors.lastname: Nakanishi
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D019556:COS Cells; D002460:Cell Line; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology; D004167:Diphtheria Toxin / Q000032:analysis / Q000493:pharmacokinetics; D019084:Fluorescent Antibody Technique, Indirect; D016679:Genome, Viral; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D008561:Membrane Fusion / Q000502:physiology*; D010446:Peptide Fragments / Q000032:analysis / Q000493:pharmacokinetics; D018108:Respirovirus / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D004167:Diphtheria Toxin; D008081:Liposomes; D010446:Peptide Fragments; C029795:diphtheria toxin fragment A