title: Suppressive effects of dominant negative ras mutant N116Y on transformed phenotypes of human bladder cancer cells.
abstract: To investigate the suppressive effect of dominant negative H-ras mutant N116Y on transformed phenotypes, we established two N116Y ras mutant stable transfectant clones (C5, C13) of human bladder cancer cell line, UMUC-2. These N116Y ras mutant transfectants, especially the C5 cells, showed a dramatic change of cellular morphology and significantly reduced growth in soft agar compared to their control. Furthermore, phosphorylation of the Jun NH2-terminal kinase (JNK) was significantly decreased in these transfectants compared to the control. These results suggest that the N116Y-induced suppression of transformed phenotypes in UMUC-2 cells is associated with inhibition of JNK phosphorylation.
authors.affiliation: Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: N
authors.initials: N
authors.lastname: Shinohara
authors.forename: A
authors.initials: A
authors.lastname: Sazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Ogiso
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Takiguchi
authors.forename: J
authors.initials: J
authors.lastname: Yasuda
authors.forename: A
authors.initials: A
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Koyanagi
authors.forename: N
authors.initials: N
authors.lastname: Kuzumaki
mesh_terms: D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002999:Clone Cells; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D005799:Genes, Dominant; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*