[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization].
OBJECTIVE
Use of monoclonal antibodies against sperm proteins in human medicine.


METHODS
Experimental and clinical studies.


METHODS
Dep. Biology and Biochemistry of Fertilization, Institute of Molecular Genetics, Prague, Laboratory IVF, Iscare IVF, Prague, Dep. of Immunobiology, Institute for the Care of Mother and Child, Prague.


METHODS
Monoclonal antibodies against human sperm intra-acrosomal and cell surface proteins were used for quantitative analysis of these proteins by the immunofluorescence test in samples of human sperm of good and poor qualities.


RESULTS
The detection of intra-acrosomal proteins was decreased and, on the other hand, detection of surface proteins was the same or higher in pathological spermatozoa.


CONCLUSIONS
Monoclonal antibodies can be used for diagnostics of sperm pathology (quantitative detection of proteins) and for evaluation of the physiological state of sperm cells (state of acrosome before or after acrosome reaction). Finally, monoclonal antibodies could be useful for selection of a suitable method of fertilization (IUI, standard IVF, ICSI) in the laboratories of assisted reproduction.
Chládek
D
D
Ustav molekulární genetiky AV CR, Praha-Krc.
Pĕknicová
J
J
Capková
J
J
Geussová
G
G
Teplá
O
O
Madar
J
J
D000177:Acrosome / Q000276:immunology; D000911:Antibodies, Monoclonal; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis / Q000276:immunology / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D012099:Reproductive Techniques; D013094:Spermatozoa / Q000276:immunology*
D000911:Antibodies, Monoclonal