title: CD10 expression in cultured human osteoblast-like cells.
abstract: Morphological features, bone nodule formation and alkaline phosphatase activity are currently used to identify osteoblasts. CD10 (cALLa antigen) is a glycoprotein with endopeptidase activity and it is present on the surface of many cell types. We have studied the expression of CD10 in osteoblast-like cells by immunocytochemistry and flow cytometry in order to identify other markers of the osteoblast lineage. We isolated osteoblast-like cells from specimens obtained in the course of oral surgery. Expression of the cALLa antigen (CD10) may also be an indicator of the osteoblast phenotype.
authors.affiliation: Departamento Estomatología, Instituto de Neurociencias, Universidad de Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Reyes-Botella
authors.forename: M J
authors.initials: MJ
authors.lastname: Montes
authors.forename: A C
authors.initials: AC
authors.lastname: Abadía-Molina
authors.forename: M F
authors.initials: MF
authors.lastname: Vallecillo-Capilla
authors.forename: C
authors.initials: C
authors.lastname: Ruiz
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D019070:Cell Lineage; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques; D015260:Neprilysin / Q000096:biosynthesis* / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism*; D015675:Osteocalcin / Q000032:analysis; D010641:Phenotype
chemical_list: D015415:Biomarkers; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D015260:Neprilysin