Isolation and identification of the probing stimulants in the rice plant for the white-back planthopper, Sogatella furcifera (homoptera: delphacidae).
Adult females of the white-back planthopper, Sogatella furcifera, showed characteristic behavior of stylet sheath deposit on a parafilm membrane when fed on a 2% aqueous crude rice leaf and stem extract containing 15% sucrose. Subsequent bioassays revealed that the butanol-soluble fraction of the extract was highly active against the insects. When the butanol fraction was chromatographed on an ODS open column and eluted in sequence with a mixture of an increasing concentration of methanol in water, the 40 % methanol fraction was separated as the most active. A further bioassay of the HPLC components in the active fraction revealed that two major components (1 and 3) stimulated the high probing activity of the white-back planthopper only when they were combined. Of the active components, one component (3) was identified to be tricin 5-O-glucoside by spectroscopic analyses.
Adjei-Afriyie
F
F
Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan.
Kim
C S
CS
Takemura
M
M
Ishikawa
M
M
Horiike
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007313:Insecta / Q000502:physiology*; D007605:Juvenile Hormones / Q000737:chemistry*; D013058:Mass Spectrometry; D012275:Oryza / Q000737:chemistry*
D007605:Juvenile Hormones