title: Isolation of avian pneumovirus from an outbreak of respiratory illness in Minnesota turkeys.
abstract: Antibodies to avian pneumovirus (APV) were first detected in Minnesota turkeys in 1997. Virus isolation was attempted on 32 samples (28 tracheal swabs, 4 pools of trachea and turbinates) that were positive for APV by reverse transcriptase polymerase chain reaction (RT-PCR). The cell cultures used were chicken embryo fibroblast (CEF), Vero cells, and QT-35 cells. Five virus isolates were obtained from these samples, and the identity of the isolates was confirmed by RT-PCR. Four isolates were obtained by inoculation of CEF cells, and 1 isolate was obtained in QT-35 cells after 3-7 blind passages in cell cultures. Vero cells did not yield any isolate on primary isolation; however, all 5 isolates could be adapted to grow in Vero cells following primary isolation in CEF or QT-35 cells. This is the first report of isolation of APV in Minnesota and also the first report of primary isolation of APV in QT-35 cells.
authors.affiliation: Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Goyal
authors.forename: S J
authors.initials: SJ
authors.lastname: Chiang
authors.forename: A M
authors.initials: AM
authors.lastname: Dar
authors.forename: K V
authors.initials: KV
authors.lastname: Nagaraja
authors.forename: D P
authors.initials: DP
authors.lastname: Shaw
authors.forename: D A
authors.initials: DA
authors.lastname: Halvorson
authors.forename: V
authors.initials: V
authors.lastname: Kapur
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000821:virology*; D002642:Chick Embryo; D004196:Disease Outbreaks / Q000662:veterinary; D008910:Minnesota / Q000453:epidemiology; D018111:Pneumovirus / Q000302:isolation & purification*; D018186:Pneumovirus Infections / Q000473:pathology / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014422:Turkeys / Q000821:virology*