A Cdc7p-Dbf4p protein kinase activity is conserved from yeast to humans.
DBF4 and CDC7 were identified as budding yeast cell cycle mutants that arrest immediately before S phase. The Dbf4p and Cdc7p proteins interact to form a protein kinase, Cdc7p being the catalytic subunit and Dbf4p is a cyclin-like molecule that activates the kinase in late G1. Dbf4p also targets Cdc7p to origins of replication where likely substrates include the Mcm proteins. Dbf4p and Cdc7p related proteins occur in the fission yeast and in metazoans. These also phosphorylate Mcm proteins and preliminary evidence indicates a similar function to Dbf4p/Cdc7p in budding yeast. The Dbf4p/Cdc7p activity will therefore very likely be conserved in all eukaryotes.
Johnston
L H
LH
Division of Yeast Genetics, National Institute for Medical Research, London, U.K.
Masai
H
H
Sugino
A
A
D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D005057:Eukaryotic Cells; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity; D015003:Yeasts
D018797:Cell Cycle Proteins; C088096:Dbf4 protein, S cerevisiae; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C489404:CDC7 protein, S cerevisiae; C068619:CDC7 protein, human; D017346:Protein Serine-Threonine Kinases