title: Production of viral antigens in culture fluid of C6/36 mosquito cell line infected with dengue type 4 virus strains isolated from patients with different clinical severities.
abstract: Viral antigen production was examined in the culture fluid of Aedes albopictus clone C6/36 cell line incubated at 28 degrees C and 37 degrees C after infection with four strains of dengue type 4 (DEN-4) virus which were isolated from patients with different clinical severities. During the observation period from day 1 to day 18, the number of infected cells at each day for all four strains did not show any significant difference (P >0.05). Antigen production as determined by the hemagglutination (HA) test and sandwich enzyme linked immunosorbent assay (ELISA) was higher at 28 degrees C than at 37 degrees C for all four DEN-4 virus strains examined. The amount of viral antigen produced was highest for CT93-74 strain (dengue hemorrhagic fever syndrome (DHF) grade II) and was significantly different in comparison to other strains (P <0.001). This strain was followed by CT93-158 and CT93-129 strains (both of DHF grade I), and CT93-77 strain (dengue fever (DF)). The viral antigen production was apparently proportional to the clinical severity of the patient from whom the virus was isolated. These results show that CT93-74 strain could be used to produce DEN-4 virus antigen of sufficiently high titer in the C6/36 cell culture instead of classical extraction of this antigen from suckling mouse brains.
authors.affiliation: Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand. phccy@mahidol.ac.th
authors.forename: C
authors.initials: C
authors.lastname: Chanyasanha
authors.forename: F
authors.initials: F
authors.lastname: Hasebe
authors.forename: R
authors.initials: R
authors.lastname: Matias
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D002460:Cell Line; D003470:Culture Media; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D019595:Severe Dengue / Q000821:virology*
chemical_list: D000956:Antigens, Viral; D003470:Culture Media