A BAC-based physical map of the major autosomes of Drosophila melanogaster.
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.
Hoskins
R A
RA
Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. hoskins@bdgp.lbl.gov
Nelson
C R
CR
Berman
B P
BP
Laverty
T R
TR
George
R A
RA
Ciesiolka
L
L
Naeemuddin
M
M
Arenson
A D
AD
Durbin
J
J
David
R G
RG
Tabor
P E
PE
Bailey
M R
MR
DeShazo
D R
DR
Catanese
J
J
Mammoser
A
A
Osoegawa
K
K
de Jong
P J
PJ
Celniker
S E
SE
Gibbs
R A
RA
Rubin
G M
GM
Scherer
S E
SE
D000818:Animals; D002503:Centromere / Q000235:genetics; D002843:Chromatin / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D020451:Contig Mapping; D016172:DNA Fingerprinting; D004331:Drosophila melanogaster / Q000235:genetics*; D022041:Euchromatin; D015723:Gene Library; D017344:Genes, Insect; D005819:Genetic Markers; D005822:Genetic Vectors; D016678:Genome; D017403:In Situ Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D016615:Telomere / Q000235:genetics
D002843:Chromatin; D022041:Euchromatin; D005819:Genetic Markers