Size-exclusion chromatography study of the molecular-weight distribution of gamma-irradiated pullulan.
The polyelectrolyte behavior of gamma-irradiated pullulan in aqueous solutions leads to secondary effects of adsorption on a Shodex OHPak KB 806 column gel during size-exclusion chromatography. Suppression of the polyelectrolyte properties of gamma-irradiated pullulan is achieved by using a 0.05 M aqueous solution of NaH2PO4 (pH 4.95) as the mobile phase. Under these conditions, adequate molecular-weight distributions of gamma-irradiated pullulan samples are obtained.
Shingel
K I
KI
Scientific Pharmaceutical Centre, Belmedpreparaty Pharmaceutical Co., Minsk, Belarus.
Tsarenkov
V M
VM
Petrov
P T
PT
D002850:Chromatography, Gel; D004573:Electrolytes; D005720:Gamma Rays; D005936:Glucans / Q000737:chemistry / Q000528:radiation effects*; D008970:Molecular Weight; D014783:Viscosity
D004573:Electrolytes; D005936:Glucans; C009109:pullulan