title: Molecular typing and exopolysaccharide biosynthesis of Burkholderia cepacia isolates from a Portuguese cystic fibrosis center.
abstract: This work describes the first epidemiological survey of Burkholderia cepacia involved in pulmonary infections among the Portuguese population with cystic fibrosis (CF) who attended the major CF treatment Center in Lisbon at Sta. Maria Hospital from 1995 to the end of 1997. The characterization of the genomic relatedness of the isolates was based on the analysis of their ribopatterns (with EcoRI) followed by construction of a ribotype-based phylogenetic tree. This study was complemented with macrorestriction fragment analysis by pulsed-field gel electrophoresis. After optimization of the solid growth medium, we found that exopolysaccharide (EPS) production by B. cepacia CF isolates is not as rare a phenomenon as was thought before; indeed, 70% of the isolates examined were EPS producers.
authors.affiliation: Centro de Engenharia Biológica e Química, Instituto Superior Técnico, 1049-001 Lisbon.
authors.forename: J A
authors.initials: JA
authors.lastname: Richau
authors.forename: J H
authors.initials: JH
authors.lastname: Leitão
authors.forename: M
authors.initials: M
authors.lastname: Correia
authors.forename: L
authors.initials: L
authors.lastname: Lito
authors.forename: M J
authors.initials: MJ
authors.lastname: Salgado
authors.forename: C
authors.initials: C
authors.lastname: Barreto
authors.forename: P
authors.initials: P
authors.lastname: Cescutti
authors.forename: I
authors.initials: I
authors.lastname: Sá-Correia
mesh_terms: D015373:Bacterial Typing Techniques; D019121:Burkholderia Infections / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011174:Portugal; D015183:Restriction Mapping
chemical_list: D011135:Polysaccharides, Bacterial; D015246:Deoxyribonuclease EcoRI