title: Detection of RFLP markers associated with antibody response in meat-type chickens: haplotype/genotype, single-band, and multiband analyses of RFLP in the major histocompatibility complex.
abstract: Improving disease resistance in poultry by direct selection or by selecting for immune response is hardly feasible due to the quantitative nature of these traits, their low heritability, and the difficulties associated with reliable measurements. In this situation, marker-assisted selection (MAS) is expected to be a more effective breeding approach. The major histocompatibility complex (MHC), known to affect immune response and disease resistance, was examined as a set of candidate genes for association between DNA markers and antibody response. Backcross (BC1) and F2 families were generated from a cross between lines divergently selected for high or low antibody response to Escherichia coli vaccination. Restriction fragment length polymorphism (RFLP) analysis of the highly polymorphic MHC class IV (B-G) region suggested an association with antibody response to several antigens (E. coli, SRBC, NDV). The multiband data generated with the class IV probe were used to compare the efficacies of three alternative analyses: "single-band" (carriers versus noncarriers of each RFLP band separately), "multiband" (multiple regression on all RFLP bands), and "genotype" (determined from family analysis of RFLP patterns/haplotypes). Groups of birds identified by the "multiband" analysis were identical to the haplotype-based genotypes, suggesting that the laborious step of haplotype determination can be omitted without unduly sacrificing power of analysis.
authors.affiliation: Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel. nyonash@ansc1.cag.uconn.edu
authors.forename: N
authors.initials: N
authors.lastname: Yonash
authors.forename: E D
authors.initials: ED
authors.lastname: Heller
authors.forename: J
authors.initials: J
authors.lastname: Hillel
authors.forename: A
authors.initials: A
authors.lastname: Cahaner
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation / Q000235:genetics*; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D003433:Crosses, Genetic; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D005838:Genotype; D006239:Haplotypes; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology; D008297:Male; D008460:Meat; D009522:Newcastle disease virus / Q000276:immunology; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep; D014612:Vaccines / Q000276:immunology / Q000494:pharmacology
chemical_list: D000906:Antibodies; D014612:Vaccines