A method to produce Ponceau replicas from blots: application for Western analysis.
We describe a fast and simple method to produce print-quality like Ponceau replicas from blots. These have many applications for Western analysis, primarily to alleviate localization of antigens in complex protein patterns generated by two-dimensional electrophoresis.
Gotzmann
J
J
Institute of Tumor Biology, Cancer Research, University of Vienna, Austria. josef.gotzmann@univie.ac.at
Gerner
C
C
D001391:Azo Compounds / Q000737:chemistry*; D015153:Blotting, Western / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional
D001391:Azo Compounds; D004396:Coloring Agents; C032756:ponceau S