Effects of ovarian hormones on beta-adrenergic receptor-mediated relaxation in the female rabbit bladder.
The effects of ovarian hormones on beta-adrenergic receptor-mediated responses in female rabbit detrusor smooth muscles were investigated. Ovariectomized mature female New Zealand white rabbits were untreated or treated with estrogen and/or progesterone for 2 weeks. The contractile responses to carbachol and KCl in the detrusor strips were not significantly different in all groups. As compared with dobutamine and GS-332, isoproterenol and procaterol significantly relaxed the detrusor strips derived from all groups on KCl-induced tonic contractions. Combined with estrogen treatment, isoproterenol, procaterol and GS-332 caused a significant increase in this muscle relaxation. Furthermore, estrogen treatment caused a significant increase in relaxation as a result of forskolin and the cyclic AMP (cAMP) production that was induced by isoproterenol, procaterol and GS-332. However, estrogen treatment did not affect the relaxant response to dibutyryl cyclic AMP. Progesterone treatment did not affect beta-adrenergic receptor-mediated responses. These results suggest that estrogen treatment causes the increased relaxant responses mediated by beta2- and beta3-adrenergic receptor subtypes, which may be related to the increased cAMP content in female rabbit detrusor smooth muscles.
Yono
M
M
Department of Urology, Kumamoto University School of Medicine, Japan.
Yoshida
M
M
Takahashi
W
W
Inadome
A
A
Seshita
H
H
Miyamoto
Y
Y
Ueda
S
S
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004280:Dobutamine / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D010642:Phenoxyacetates / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D017265:Procaterol / Q000494:pharmacology; D011374:Progesterone / Q000502:physiology*; D011817:Rabbits; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
D000318:Adrenergic beta-Agonists; D004967:Estrogens; C119908:GS 332; D010642:Phenoxyacetates; D004280:Dobutamine; D011374:Progesterone; D011189:Potassium Chloride; D002217:Carbachol; D007545:Isoproterenol; D017265:Procaterol