Mass spectrometry in viral proteomics.
Mass spectrometry is a valuable tool in structural and functional viral proteomics, where it has been used to identify viral capsid proteins, viral mutants, and posttranslational modifications. Further, mass-based approaches combined with time-resolved proteolysis (mass mapping) have revealed the dynamic nature of viral particles in solution; this method is contributing to an understanding of the dynamic domains of the viral capsid which may have significant value in developing new approaches for viral inactivation. As a result of these experiments, and by comparison with complementary data from X-ray crystallography, a new dimension to viral protein structure and function is emerging.
Thomas
J J
JJ
Beckman Center for Chemical Sciences, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. jthomas@scripps.edu
Bakhtiar
R
R
Siuzdak
G
G
D013058:Mass Spectrometry / Q000379:methods*; D011487:Protein Conformation; D020543:Proteome / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
D020543:Proteome; D014764:Viral Proteins