Lysyl-tRNA synthetase of Bacillus stearothermophilus molecular cloning and expression of the gene.
The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids. The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.
Takita
T
T
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Japan.
Shimizu
N
N
Sukata
T
T
Hashimoto
S
S
Akita
E
E
Yokota
T
T
Esaki
N
N
Soda
K
K
Inouye
K
K
Tonomura
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D003062:Codon; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D008250:Lysine-tRNA Ligase