Promotion of hepatocarcinogenesis by hexachlorobenzene in energy-restricted rats.
The interaction between dietary energy restriction and low dose of the fungicide hexachlorobenzene (HCB) was evaluated in a rat liver medium-term bioassay for carcinogenesis. Male Wistar rats were fed a control or a 50% energy-restricted diet, both added or not with 50 ppm HCB, for 6 weeks. HCB exposure or energy restriction separately did not exert any influence on the development of glutathione S-transferase placental form (GST-P(+)) foci of hepatocytes. Simultaneous HCB exposure and energy restriction induced a significant increase in liver centrilobular hypertrophy and GST-P(+) foci development. Our findings suggest that energy restriction increases liver response to low dose of HCB, unmasking the promoting potential of this fungicide.
Kishima
M O
MO
Departamento de Patologia Aplicada, Legislação e Deontologia, Faculdade de Medicina, UEL, Londrina, PR, Brazil.
Barbisan
L F
LF
Estevão
D
D
Rodrigues
M A
MA
Viana de Camargo
J L
JL
D000818:Animals; D001835:Body Weight; D003043:Cocarcinogenesis; D002149:Energy Intake; D005659:Fungicides, Industrial / Q000633:toxicity; D006581:Hexachlorobenzene / Q000633:toxicity*; D008099:Liver / Q000033:anatomy & histology / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D008297:Male; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar
D005659:Fungicides, Industrial; D006581:Hexachlorobenzene