Preparation, evaluation and application of new pseudo-affinity chromatographic supports for penicillin acylase purification.
New pseudo-affinity chromatographic supports for penicillin acylase were prepared and evaluated with three different samples: pure penicillin acylase, industrial clarified feedstock and crude extract. The different gels were studied for their purification fold (three to six) and their recovery power (80-100%). The best support was characterized by its dynamic capacity, (20 mg/ml) and its recovery power was tested at five flow-rates (30, 150, 300 and 750 cm/h) to determine the optimal flow-rate (300 cm/h). In addition we used cleaning in place to test the resistance to hard conditions of sanitization by 1 M NaOH (90% of recovery for 12 h of contact). These gels may therefore be used on an industrial scale.
Santarelli
X
X
Ecole Supérieure de Technologie des Biomolécules de Bordeaux, Université Victor Segalen Bordeaux 2, France. xavier.santarelli@biomemb.u-bordeaux2.fr
Fitton
V
V
Verdoni
N
N
Cassagne
C
C
D002846:Chromatography, Affinity / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry; D005069:Evaluation Studies as Topic; D008024:Ligands; D010399:Penicillin Amidase / Q000302:isolation & purification*
D008024:Ligands; D010399:Penicillin Amidase