Biosynthesis and secretion of several enzymes in Escherichia coli dnaK and dnaJ mutants.
Escherichia coli null dnaJ and dnaKdnaJ mutants were defective in the biosynthesis and secretion of several enzymes. The synthesis of beta-galactosidase induced in delta dnaJ and delta dnaKdnaJ mutants was abolished at 42 degrees C and significantly decreased at 30 and 37 degrees C. The activity of alkaline phosphatase in the periplasm in both mutant strains at high temperature was lower than in the wild-type strain. The synthesis of b-type cytochromes was defective in two deletion mutants while the synthesis of nitrate reductase-A at 42 degrees C was influenced by dnaK mutation only. The lack of DnaK and DnaJ does not impair the activity of catechol 2,3-dioxygenase irrespective of growth temperature.
Wolska
K I
KI
Department of Bacterial Genetics, Warsaw University, Poland.
Lobacz
B
B
Jurkiewicz
D
D
Bugajska
E
E
Kuć
M
M
Jóźwik
A
A
D000469:Alkaline Phosphatase / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism; D050557:Catechol 2,3-Dioxygenase; D003573:Cytochrome b Group / Q000096:biosynthesis / Q000378:metabolism; D049308:Dioxygenases; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006361:Heating; D018832:Molecular Chaperones / Q000235:genetics*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000096:biosynthesis / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000378:metabolism
D001426:Bacterial Proteins; D003573:Cytochrome b Group; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase; C063842:dnaK protein, E coli