[Closely relationship between expression of endoplasmic reticulum molecular chaperone Grp94 and c-myc oncogene in human colorectal carcinoma cell lines].
OBJECTIVE
To study the biological significance of Grp94 deleted product (Grp94 beta) expressed in human colorectal carcinoma cells.


METHODS
The relationship between molecular chaperone Grp94 and c-myc oncogene expression in the human colorectal carcinoma cell line CCL229, CX-1 and retinoic acid (RA) induced CCL229 by both of digoxin labelled c-myc cDNA probe spot by-bridization and RT-PCR with endoplasmic reticulum molecular chaperone Grp94 proximal 3' end oligonucletide primers was investigated.


RESULTS
In CCL229 cells, the expression of Grp94 beta and c-myc oncogene are significantly higher than those in CX-1 cells. Along with the RA inducing, both decreased with one accord, and to the lowest level after six days induction.


CONCLUSIONS
The Grp94 normal expression (Grp94 alpha), which was undetectable in CCL229 cells increased after RA induction. The results showed that the expression of Grp94 beta may be closely related to that of c-myc oncogene. It is suggested that the Grp94 be related to some biological characteristics of cancer, for example, the invasiveness.
Chen
Y
Y
Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang.
Song
J
J
D000970:Antineoplastic Agents / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C018551:glucose-regulated proteins; D014212:Tretinoin