title: Type II and type III monodeiodinase activities in the skin of untreated and propylthiouracil-treated cashmere goats.
abstract: The presence or absence of types I, II and III iodothyronine monodeiodinase enzymes (MDI, MDII and MDIII) and their levels of activity in the skin of goats, which were orally dosed for 60 days with 0, 1.1, 2.2, 4.4, 8.8, 17.5, or 35 mg(-1)kg liveweight day(-1)of the anti-thyroid, enzyme-inhibiting drug, propylthiouracil (PTU), were determined. Contrary to our earlier report that PTU did not influence skin MDII activity, the currect more thorough investigation (in terms of numbers of observations and the efficiency of the enzyme extraction procedure) indicated that doses of 1.1.to 17.5 mg kg(-1)liveweight induced a 2 to 3 fold increase (P = 0.01) in MDII activity. However, in three of the four goats treated with 35 mg kg(-1)group, activity was similar to that of control animals. There were no significant differences between treatments in MDIII activity but there was a trend towards lower levels of activity in the goats dosed with 17.5 and 35 mg kg(-1). It is concluded that there is significant MDII and MDIII activity in the skin of goats and that although there is none of the PTU -sensitive MDI enzyme, synthesis of T3 within the skin could nevertheless be modified through increases in MDII activity induced by lower T4 concentrations in the circulation caused by PTU. Changes in pattern of fibre moult induced by treatment with low doses of MD-inhibiting drugs may therefore be achieved through this effect. Since MDII and MDIII enzyme activity may be reduced by high doses of PTU, prolonged treatment with high doses of PTU may have adverse effects on skin tissue.
authors.affiliation: Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen, AB15 8QH.
authors.forename: D
authors.initials: D
authors.lastname: Villar
authors.forename: F
authors.initials: F
authors.lastname: Nicol
authors.forename: J R
authors.initials: JR
authors.lastname: Arthur
authors.forename: P
authors.initials: P
authors.lastname: Dicks
authors.forename: A
authors.initials: A
authors.lastname: Cannavan
authors.forename: D G
authors.initials: DG
authors.lastname: Kennedy
authors.forename: S M
authors.initials: SM
authors.lastname: Rhind
mesh_terms: D000818:Animals; D002369:Castration; D004305:Dose-Response Relationship, Drug; D006041:Goats / Q000378:metabolism*; D007453:Iodide Peroxidase / Q000378:metabolism*; D008297:Male; D011441:Propylthiouracil / Q000008:administration & dosage / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000201:enzymology*; D000097564:Iodothyronine Deiodinase Type II
chemical_list: D007453:Iodide Peroxidase; D011441:Propylthiouracil; D000097564:Iodothyronine Deiodinase Type II; C117274:iodothyronine deiodinase type III