From sequence to chromosome: the tip of the X chromosome of D. melanogaster.
One of the rewards of having a Drosophila melanogaster whole-genome sequence will be the potential to understand the molecular bases for structural features of chromosomes that have been a long-standing puzzle. Analysis of 2.6 megabases of sequence from the tip of the X chromosome of Drosophila identifies 273 genes. Cloned DNAs from the characteristic bulbous structure at the tip of the X chromosome in the region of the broad complex display an unusual pattern of in situ hybridization. Sequence analysis revealed that this region comprises 154 kilobases of DNA flanked by 1.2-kilobases of inverted repeats, each composed of a 350-base pair satellite related element. Thus, some aspects of chromosome structure appear to be revealed directly within the DNA sequence itself.
Benos
P V
PV
The European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton Hall, Cambridge CB10 1SD, UK.
Gatt
M K
MK
Ashburner
M
M
Murphy
L
L
Harris
D
D
Barrell
B
B
Ferraz
C
C
Vidal
S
S
Brun
C
C
Demailles
J
J
Cadieu
E
E
Dreano
S
S
Gloux
S
S
Lelaure
V
V
Mottier
S
S
Galibert
F
F
Borkova
D
D
Minana
B
B
Kafatos
F C
FC
Louis
C
C
Sidén-Kiamos
I
I
Bolshakov
S
S
Papagiannakis
G
G
Spanos
L
L
Cox
S
S
Madueño
E
E
de Pablos
B
B
Modolell
J
J
Peter
A
A
Schöttler
P
P
Werner
M
M
Mourkioti
F
F
Beinert
N
N
Dowe
G
G
Schäfer
U
U
Jäckle
H
H
Bucheton
A
A
Callister
D M
DM
Campbell
L A
LA
Darlamitsou
A
A
Henderson
N S
NS
McMillan
P J
PJ
Salles
C
C
Tait
E A
EA
Valenti
P
P
Saunder
R D
RD
Glover
D M
DM
D000818:Animals; D002871:Chromosome Banding; D019295:Computational Biology; D003360:Cosmids; D004251:DNA Transposable Elements; D004276:DNA, Satellite; D004331:Drosophila melanogaster / Q000235:genetics*; D017344:Genes, Insect; D017403:In Situ Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D014960:X Chromosome / Q000235:genetics* / Q000648:ultrastructure
D004251:DNA Transposable Elements; D004276:DNA, Satellite