Cyborg lectins: novel leguminous lectins with unique specificities.
Bauhinia purpurea lectin (BPA) is one of the beta-galactose-binding leguminous lectins. Leguminous lectins contain a long metal-binding loop, part of which determines their carbohydrate-binding specificities. Random mutations were introduced into a portion of the cDNA coding BPA that corresponds to the carbohydrate-binding loop of the lectin. An library of the mutant lectin expressed on the surface of lambda foo phages was screened by the panning method. Several phage clones with an affinity for mannose or N-acetylglucosamine were isolated. These results indicate the possibility of making artificial lectins (so-called "cyborg lectins") with distinct and desired carbohydrate-binding specificities.
Yamamoto
K
K
Laboratory of Molecular Medicine, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Japan. yamamoto@k.u-tokyo.ac.jp
Maruyama
I N
IN
Osawa
T
T
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D010582:Bacteriophage lambda / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D007887:Fabaceae / Q000737:chemistry / Q000235:genetics*; D005822:Genetic Vectors / Q000138:chemical synthesis; D037102:Lectins / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000235:genetics* / Q000378:metabolism; D008358:Mannose / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D037121:Plant Lectins; D010940:Plant Proteins / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000235:genetics* / Q000378:metabolism; D010946:Plants, Medicinal; D015202:Protein Engineering; D012709:Serum Albumin / Q000235:genetics / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000378:metabolism
C039340:Bauhinia purpurea lectin; D037102:Lectins; D008565:Membrane Proteins; C050080:N-acetylglucosamine-bovine serum albumin conjugate; D010446:Peptide Fragments; D019151:Peptide Library; D037121:Plant Lectins; D010940:Plant Proteins; D012709:Serum Albumin; C023840:mannose-bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D008358:Mannose; D000117:Acetylglucosamine