Pulsatility of systemic FSH and LH concentrations during follicular-wave development in cattle.
Changes in systemic FSH and LH pulsatility in temporal association with follicular-wave emergence and follicle deviation were studied in cattle. Wave emergence was defined as occurring when the future dominant follicle first reached 4 mm, as established retrospectively. Follicle deviation was defined as the beginning of a change in growth rates between the 2 largest follicles and occurred 60.4 +/- 4.2 h after wave emergence. Follicles were tracked by transrectal ultrasound scanning every 8 h, and blood samples for pulse characterization were collected every 20 min from before emergence until after deviation. Pulses were characterized by the Pulsar program applied to each 8-h increment, centered on the hour of follicle scanning in each heifer (n = 6). Pulsatility of FSH was not detected for any of the 8-h increments. The mean FSH concentrations for the 24 samples per 8 h increased (P < 0.05) between 8 h before and 8 h after wave emergence, followed by a decrease 40 to 16 h before deviation. The low mean values continued for 24 h after deviation. Pulses of LH were detected for all 8-h increments. The LH mean of all concentrations per 8 h and pulse frequency increased (P < 0.05) between the hour of wave emergence and 32 h after emergence and then pulse frequency plateaued at a mean interpeak interval of 1.3 h. Increased LH means for all concentrations per 8 h and basal concentration were reached 32 h before deviation. The results indicated that elevated concentrations of LH and reduced concentrations of FSH were present 32 to 16 h before to at least 24 h after the beginning of follicle deviation. However, an abrupt, short-term change in FSH concentrations or in LH pulsatility in close temporal association with follicle deviation that could act as an acute stimulus to initiate deviation was not found.
Ginther
O J
OJ
University of Wisconsin-Madison 53706, USA.
Bergfelt
D R
DR
Kulick
L J
LJ
Kot
K
K
D000818:Animals; D002417:Cattle / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D007986:Luteinizing Hormone / Q000097:blood*; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000502:physiology*; D010507:Periodicity; D014463:Ultrasonography
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015237:Dinoprost