Biomonitoring of environmental polycyclic aromatic hydrocarbon exposure by simultaneous measurement of urinary phenanthrene, pyrene and benzo[a]pyrene hydroxides.
A high-performance liquid chromatographic method with fluorescence detection was developed which enables the simultaneous determination of the urinary polycyclic aromatic hydrocarbon metabolites 3-hydroxyphenanthrene, 1-hydroxypyrene and 3-hydroxybenzo[a]pyrene. The method has small solvent consumption because of the use of a microbore RP C18 column and a relatively short run time. Low detection limits of 0.02 nmol/l for 3-hydroxypyrene to 0.19 nmol/l for 3-hydroxybenzo[a]pyrene were attained. In contrast, the detection limits of alpha-naphthol and 9,10-dihydroxy-9,10-dihydrophenanthrene were not adequate for the determination of environmental exposure. The developed method was successfully used for the analysis of urine samples from children.
Hollender
J
J
Institute of Hygiene and Environmental Medicine, RWTH Aachen, Germany. juliane.hollender@post.rwth-aachen.de
Koch
B
B
Dott
W
W
D000393:Air Pollutants / Q000032:analysis / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000032:analysis* / Q000737:chemistry; D015415:Biomarkers / Q000652:urine; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004781:Environmental Exposure; D006801:Humans; D010616:Phenanthrenes / Q000652:urine*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000652:urine*; D011721:Pyrenes / Q000032:analysis*; D012140:Respiratory Tract Diseases / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods
D000393:Air Pollutants; D015415:Biomarkers; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D001564:Benzo(a)pyrene