title: Crystallization and initial X-ray analysis of alkaline xylanase.
abstract: Crystals of alkaline xylanase (xylanase J) from alkaliphilic Bacillus sp. 41M-1 were grown by decreasing the temperature of the protein solution. Initial X-ray analysis of the crystal showed a tetragonal system with space group P4(1) or P4(3) and unit-cell parameters a = b = 115.7, c = 46.0 A. Assuming two molecules per asymmetric unit, V(m) = 2.31 A(3) Da(-1). The crystals diffract X-rays to 2.6 A resolution at 100 K.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Lu
authors.forename: H
authors.initials: H
authors.lastname: Moriyama
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D001407:Bacillus / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase