An HPLC assay for the determination of ketoconazole in common pharmaceutical preparations.
An HPLC method is described using octadecylsilica (3 microns) with an acetonitrile phosphate buffer mobile phase containing diethylamine which is capable of separating ketoconazole [(+/-)-cis-1-acetyl-4-(4[2-(dichlorophenyl)-2- (1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl)pi perazine] from four related compounds, (R049223, R063600, R053165 and R039519) and from excipients in tablets, cream and shampoo. The method was validated using an external calibration method for tablets, shampoo and creams and a standard addition method for cream. The limits of detection for the related compounds in the presence of ketoconazole are also reported.
Low
A S
AS
School of Pharmacy, Robert Gordon University, Schoolhill, Aberdeen, UK.
Wangboonskul
J
J
D000935:Antifungal Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006203:Hair Preparations / Q000737:chemistry; D007654:Ketoconazole / Q000032:analysis*; D009824:Ointments; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013607:Tablets
D000935:Antifungal Agents; D006203:Hair Preparations; D009824:Ointments; D004364:Pharmaceutical Preparations; D013607:Tablets; D007654:Ketoconazole