title: Escherichia coli O157:H7 induces attaching-effacing lesions in large intestinal mucosal explants from adult cattle.
abstract: Attaching-effacing (A/E) lesions following natural and experimental infection with Escherichia coli O157:H7 have been seen in neonatal and 3-4-month-old weanling but not older cattle. To test the hypothesis that the adult bovine large intestinal epithelium is resistant to the development of A/E lesions, colonic and rectal mucosal tissue explants from 18-month-old steers were inoculated with E. coli O157:H7 and examined. Epithelial cells of inoculated explants developed A/E lesions at the bacterial attachment sites, providing evidence that the large intestinal mucosal epithelium may be a site of infection that contributes to carriage of E. coli O157:H7 in adult cattle.
authors.affiliation: Department of Veterinary Sciences, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Baehler
authors.forename: R A
authors.initials: RA
authors.lastname: Moxley
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology; D003106:Colon / Q000382:microbiology / Q000648:ultrastructure; D046508:Culture Techniques / Q000379:methods; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000472:pathogenicity*; D007413:Intestinal Mucosa / Q000382:microbiology* / Q000648:ultrastructure; D007420:Intestine, Large / Q000382:microbiology* / Q000648:ultrastructure; D012007:Rectum / Q000382:microbiology / Q000648:ultrastructure