Determination of quinolones in animal tissues and eggs by high-performance liquid chromatography with photodiode-array detection.
A rapid, specific reversed-phase HPLC method is described, with solid-phase extraction, for assaying five quinolones (ciprofloxacin, difloxacin, enrofloxacin, norfloxacin and marbofloxacin) with confirmative diode-array detection in samples of bovine kidney, muscle and eggs. The least efficient extraction was marbofloxacin from kidney tissue (64%). The lower detection limit for each quinolone was: enrofloxacin and ciprofloxacin, 1 ng; norfloxacin and difloxacin, 2 ng; marbofloxacin, 4 ng injected. The intra-day relative standard deviations were lower than 7.9% and lower than 8.6% for inter-day assays. These results indicate that the developed method had an acceptable precision.
Gigosos
P G
PG
Laboratorio de Saude Pública, Consellería de Sanidade e Servicios Sociais, Lugo, Spain.
Revesado
P R
PR
Cadahía
O
O
Fente
C A
CA
Vazquez
B I
BI
Franco
C M
CM
Cepeda
A
A
D042462:4-Quinolones; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D042462:4-Quinolones; D000890:Anti-Infective Agents