Nanopores and nucleic acids: prospects for ultrarapid sequencing.
DNA and RNA molecules can be detected as they are driven through a nanopore by an applied electric field at rates ranging from several hundred microseconds to a few milliseconds per molecule. The nanopore can rapidly discriminate between pyrimidine and purine segments along a single-stranded nucleic acid molecule. Nanopore detection and characterization of single molecules represents a new method for directly reading information encoded in linear polymers. If single-nucleotide resolution can be achieved, it is possible that nucleic acid sequences can be determined at rates exceeding a thousand bases per second.
Deamer
D W
DW
Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA. deamer@hydrogen.ucsc.edu
Akeson
M
M
D001427:Bacterial Toxins / Q000737:chemistry; D005544:Forecasting; D006460:Hemolysin Proteins / Q000737:chemistry; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry; D011687:Purines / Q000737:chemistry; D011743:Pyrimidines / Q000737:chemistry; D012313:RNA / Q000032:analysis; D017421:Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D009696:Nucleic Acids; D011687:Purines; D011743:Pyrimidines; C021091:staphylococcal alpha-toxin; D012313:RNA; C030986:pyrimidine; C030985:purine