Human aortocoronary grafts and nitric oxide release: relationship to pulsatile pressure.
BACKGROUND
Short and long-term failure of saphenous vein grafts continues to be a significant problem for cardiac surgeons. The purpose of this study was to elucidate the early adaptive changes of human artery and vein conduits with respect to nitric oxide (NO) production under various pressure and pulsatile distention conditions.


METHODS
Real-time amperometric NO determinations were made in an in vitro model using human saphenous vein segments (n = 12) and internal thoracic artery segments (n = 8) between 70 and 170 mm Hg, under static conditions recorded with a pressure transducer. Exposing the tissue to morphine (10(-6) M) also stimulated NO release. Under conditions in which the conduits were exposed to the respective pressures for 1 hour, they were then examined for their granulocyte-adhering potential using computer-assisted imaging techniques.


RESULTS
A pressure-dependent decrease of NO release was found after 32 minutes of pulsatile pressure (170 mm Hg) in artery and vein, the latter of which appeared to be affected more negatively (p < 0.05; because many more observation points differed significantly after 32 minutes compared to 110 mm Hg values). In vessels maintained for 1 hour at these different pressures and then exposed to morphine (1 microM), stimulated NO release significantly diminished in the veins (artery 37.4 nM NO versus vein 18.1 nM NO; p < 0.05). Increased pressures also correlated with an increase in granulocyte adhesion to veins that could not be reduced following morphine exposure.


CONCLUSIONS
Increased pressure and cyclic distention lead to loss of NO release and increased immunocyte adhesion, which are significantly more pronounced in saphenous vein than in internal thoracic artery, suggesting that in the long term this may contribute to the failure of saphenous vein conduits in coronary revascularization.
Bilfinger
T V
TV
Department of Surgery, State University of New York at Stony Brook, 11794-8191, USA. bilfinge@surg.som.sunysb.edu
Stefano
G B
GB
D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000378:metabolism / Q000601:surgery; D006801:Humans; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000378:metabolism*; D011312:Pressure; D011673:Pulsatile Flow; D012501:Saphenous Vein / Q000378:metabolism* / Q000637:transplantation; D013895:Thoracic Arteries / Q000378:metabolism* / Q000637:transplantation
D009569:Nitric Oxide