Identification of Trichoderma strains from building materials by ITS1 ribotyping, UP-PCR fingerprinting and UP-PCR cross hybridization.
To study the role of Trichoderma in sick building syndrome, it is essential to be able to accurately identify species. Forty-four strains of Trichoderma spp. isolated from Danish buildings damaged by water leaks were identified using ITS1 ribotyping and universally primed PCR, UP-PCR. Ribotyping allowed the assignment of the strains into three distinct groups. High similarity of UP-PCR banding profiles of the strains allowed species designation for almost all strains (43 out of 44) when compared with the UP-PCR banding profiles obtained from reference strains of T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum and T. viride. However, cross hybridization of UP-PCR products showed that the latter strain had high DNA homology to the ex-type strain of T. hamatum. The combined approach is a convenient way for reliable identification of Trichoderma strains.
Lübeck
M
M
Department of Plant Biology, Plant Pathology Section, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark. mette.lubeck@plbio.kvl.dk
Poulsen
S K
SK
Lübeck
P S
PS
Jensen
D F
DF
Thrane
U
U
D016172:DNA Fingerprinting; D003718:Denmark; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D020459:Genes, rRNA / Q000235:genetics; D015151:Immunoblotting / Q000379:methods; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D018877:Sick Building Syndrome / Q000382:microbiology*; D014242:Trichoderma / Q000145:classification* / Q000302:isolation & purification*
D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases