title: The alpha(E)C domain of human fibrinogen-420 is a stable and early plasmin cleavage product.
abstract: Human fibrinogen-420, (alpha(E)betagamma)(2), was isolated from plasma and evaluated for its ability to form clots and for its susceptibility to proteolysis. Clotting parameters, including cross-linking of subunit chains, of this subclass and of the more abundant fibrinogen-340 (alphabetagamma)(2), were found to be similar, suggesting little impact of the unique alpha(E)C domains of fibrinogen-420 on coagulation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of plasmic digestion patterns revealed production from fibrinogen-420 of the conventional fibrinogen degradation products, X, Y, D, and E, to be comparable to that from fibrinogen-340 in all respects except the presence of at least 2 additional cleavage products that were shown by Western blot analysis to contain the alpha(E)C domain. One was a stable fragment (alpha(E)CX) comigrating with a 34-kd yeast recombinant alpha(E)C domain, and the other was an apparent precursor. Their release occurred early, before that of fragments D and E. Two bands of the same mobility and antibody reactivity were found in Western blots of plasma collected from patients with myocardial infarction shortly after the initiation of thrombolytic therapy.
authors.affiliation: Lindsley F. Kimball Research Institute of the New York Blood Center, New York, NY, USA.
authors.forename: D
authors.initials: D
authors.lastname: Applegate
authors.forename: L S
authors.initials: LS
authors.lastname: Steben
authors.forename: K M
authors.initials: KM
authors.lastname: Hertzberg
authors.forename: G
authors.initials: G
authors.lastname: Grieninger
mesh_terms: D000818:Animals; D001777:Blood Coagulation; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism*; D005340:Fibrinogen / Q000302:isolation & purification / Q000378:metabolism*; D005341:Fibrinolysin / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009203:Myocardial Infarction / Q000097:blood; D010446:Peptide Fragments / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D013917:Thrombin / Q000378:metabolism; D011503:Transglutaminases / Q000378:metabolism
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D010446:Peptide Fragments; D011108:Polymers; D005340:Fibrinogen; D011503:Transglutaminases; D013917:Thrombin; D005341:Fibrinolysin