title: Differentiating between memory and effector CD8 T cells by altered expression of cell surface O-glycans.
abstract: Currently there are few reliable cell surface markers that can clearly discriminate effector from memory T cells. To determine if there are changes in O-glycosylation between these two cell types, we analyzed virus-specific CD8 T cells at various time points after lymphocytic choriomeningitis virus infection of mice. Antigen-specific CD8 T cells were identified using major histocompatibility complex class I tetramers, and glycosylation changes were monitored with a monoclonal antibody (1B11) that recognizes O-glycans on mucin-type glycoproteins. We observed a striking upregulation of a specific cell surface O-glycan epitope on virus-specific CD8 T cells during the effector phase of the primary cytotoxic T lymphocyte (CTL) response. This upregulation showed a strong correlation with the acquisition of effector function and was downregulated on memory CD8 T cells. Upon reinfection, there was again increased expression of this specific O-glycan epitope on secondary CTL effectors, followed once more by decreased expression on memory cells. Thus, this study identifies a new cell surface marker to distinguish between effector and memory CD8 T cells. This marker can be used to isolate pure populations of effector CTLs and also to determine the proportion of memory CD8 T cells that are recruited into the secondary response upon reencounter with antigen. This latter information will be of value in optimizing immunization strategies for boosting CD8 T cell responses.
authors.affiliation: Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors.forename: L E
authors.initials: LE
authors.lastname: Harrington
authors.forename: M
authors.initials: M
authors.lastname: Galvan
authors.forename: L G
authors.initials: LG
authors.lastname: Baum
authors.forename: J D
authors.initials: JD
authors.lastname: Altman
authors.forename: R
authors.initials: R
authors.lastname: Ahmed
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology*; D007700:Kinetics; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011134:Polysaccharides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D011134:Polysaccharides