Meltrin beta (ADAM19) gene: cloning, mapping, and analysis of the regulatory region.
Meltrin beta (ADAM19) is a member of the metalloprotease-disintegrin family. We report here chromosomal mapping of the mouse and rat meltrin beta genes and cloning and analysis of the mouse upstream regulatory regions. The meltrin beta transcript shows a spatially and temporally restricted expression pattern during morphogenesis, indicating that the actions of this membrane-bound protease are regulated, at least in part, at the transcriptional level. Analysis of the promoter revealed positive and negative regulatory regions upstream of the gene. The former includes a GC-box that appears to be a critical cis-element for activation of the promoter in muscle cells.
Kurohara
K
K
Department of Cell Biology, The Metropolitan Institute of Medical Science, Honkomagome 3-18-22, Bunkyo-ku, Tokyo, 113-8613, Japan.
Matsuda
Y
Y
Nagabukuro
A
A
Tsuji
A
A
Amagasa
T
T
Fujisawa-Sehara
A
A
D051722:ADAM Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019483:Disintegrins; D049452:Green Fluorescent Proteins; D017404:In Situ Hybridization, Fluorescence; D008164:Luminescent Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D008666:Metalloendopeptidases; D045726:Metalloproteases; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid
D018076:DNA, Complementary; D019483:Disintegrins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D009124:Muscle Proteins; D049452:Green Fluorescent Proteins; D045726:Metalloproteases; D051722:ADAM Proteins; C411902:ADAM19 protein, human; C096116:Adam19 protein, mouse; D008666:Metalloendopeptidases