Study of vitamin E net mass transfer between alpha-tocopherol-enriched HDL and erythrocytes: application to asymptomatic hypercholesterolemic men.
We previously showed that hypercholesterolemic asymptomatic men had lower erythrocyte vitamin E content, despite normal plasma concentrations compared to normocholesterolemic men. We hypothesized that the reduced erythrocyte vitamin E concentration could be due to an impairment of transfer of vitamin E from plasma lipoproteins. We first developed a model for testing the ability of erythrocytes to accept vitamin E from high-density lipoproteins (HDL) pre-enriched in vitamin E, which allows to measure a net mass transfer of vitamin E from HDL to erythrocytes. Vitamin E-enriched HDL were obtained in controlled conditions of concentration and incubation time with a good reproducibility (CV </= 10%). The kinetic study of the net mass transfer of vitamin E to erythrocytes of healthy volunteers shows small inter- and intraindividual variations. The application of this model to erythrocytes of hyper- and normocholesterolemic men demonstrates that the reduced erythrocyte vitamin E content observed in hypercholesterolemic men was not due to a reduced ability of these cells to accept vitamin E from HDL. It might rather be due to an impairment of lipoproteins in the delivery of vitamin E to tissues, or to an oxidative stress which consumes antioxidants.
Simon
E
E
Laboratoire de Biochimie Appliquée, Faculté des Sciences pharmaceutigques et biologiques, Châtenay-Malabry, France.
Paul
J L
JL
Atger
V
V
Simon
A
A
Moatti
N
N
D001693:Biological Transport, Active; D016022:Case-Control Studies; D004912:Erythrocytes / Q000378:metabolism*; D005609:Free Radicals / Q000097:blood; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D066298:In Vitro Techniques; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008954:Models, Biological; D018384:Oxidative Stress; D014810:Vitamin E / Q000097:blood*
D005609:Free Radicals; D008075:Lipoproteins, HDL; D014810:Vitamin E