Ribotyping and rapid identification of Staphylococcus xylosus by 16-23S spacer amplification.
Ninety-five strains of Staphylococcus xylosus isolated from goat milk, French sausage or mice were analyzed together with 35 Staphylococcus type strains by 16-23S spacer amplification and ribotyping. The results obtained by PCR amplification of the 16-23S spacer region permitted the distinction of each type strain and additionally generated a DNA banding pattern characteristic for 93 of the 95 Staphylococcus xylosus strains. Ribotyping proved to be an efficient epidemiological tool for Staphylococcus xylosus species as it clustered the 95 strains into 23 distinct types.
Villard
L
L
Ecole Nationale Vétérinaire de Lyon, 1 Avenue Bourgelat, BP 83, 69280, Marcy l'Etoile, France. l.villard@vet-lyon.fr
Kodjo
A
A
Borges
E
E
Maurin
F
F
Richard
Y
Y
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D006041:Goats; D006801:Humans; D008461:Meat Products / Q000382:microbiology; D051379:Mice; D008892:Milk / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D015183:Restriction Mapping; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D015247:Deoxyribonuclease HindIII