title: Crystal structure of the cysteine-rich domain of mannose receptor complexed with a sulfated carbohydrate ligand.
abstract: The macrophage and epithelial cell mannose receptor (MR) binds carbohydrates on foreign and host molecules. Two portions of MR recognize carbohydrates: tandemly arranged C-type lectin domains facilitate carbohydrate-dependent macrophage uptake of infectious organisms, and the NH(2)-terminal cysteine-rich domain (Cys-MR) binds to sulfated glycoproteins including pituitary hormones. To elucidate the mechanism of sulfated carbohydrate recognition, we determined crystal structures of Cys-MR alone and complexed with 4-sulfated-N-acetylgalactosamine at 1.7 and 2.2 A resolution, respectively. Cys-MR folds into an approximately three-fold symmetric beta-trefoil shape resembling fibroblast growth factor. The sulfate portions of 4-sulfated-N-acetylgalactosamine and an unidentified ligand found in the native crystals bind in a neutral pocket in the third lobe. We use the structures to rationalize the carbohydrate binding specificities of Cys-MR and compare the recognition properties of Cys-MR with other beta-trefoil proteins.
authors.affiliation: Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: A J
authors.initials: AJ
authors.lastname: Chirino
authors.forename: Z
authors.initials: Z
authors.lastname: Misulovin
authors.forename: C
authors.initials: C
authors.lastname: Leteux
authors.forename: T
authors.initials: T
authors.lastname: Feizi
authors.forename: M C
authors.initials: MC
authors.lastname: Nussenzweig
authors.forename: P J
authors.initials: PJ
authors.lastname: Bjorkman
mesh_terms: D000116:Acetylgalactosamine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002236:Carbohydrate Conformation; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002461:Cell Line, Transformed; D018360:Crystallography, X-Ray; D003545:Cysteine; D006801:Humans; D037181:Lectins, C-Type; D008024:Ligands; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D037181:Lectins, C-Type; D008024:Ligands; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C046735:N-acetylgalactosamine 4-sulfate; D003545:Cysteine; D000116:Acetylgalactosamine