title: Effects of antioxidants on the development of bovine IVM/IVF embryos in various concentrations of glucose.
abstract: This study was undertaken to determine the effects of glucose, antioxidants and different oxygen tensions on the development of bovine embryos cultured in modified synthetic oviduct fluid (m-SOF) medium. In vitro matured (IVM) and fertilized (IVF) oocytes were incubated for 48 h. Embryos reaching at least the 4-cell stage were selected for further culture under various conditions for 6 d. Supplementing the m-SOF media with 4.5 mM glucose resulted in a significantly lower (P < 0.01) embryo developmental rate (21%; Day 8) than was obtained with 1.5 mM glucose (58%; Day 8) or no glucose (53%; Day 8). Antioxidants such as SOD, catalase and mannitol had no positive effect on embryo development in m-SOF medium supplemented with 1.5 mM glucose. However, in m-SOF medium supplemented with 4.5 mM glucose, SOD and mannitol significantly (P < 0.05) improved embryo development: SOD increased the developmental rate from 19 to 35% (Day 8), while mannitol increased it from 13 to 30% (Day 8). Low oxygen concentration improved embryo development significantly (P < 0.05) in m-SOF medium supplemented with 4.5 mM glucose (low O2: 31% vs high O2: 14%; Day 8) but not 0 mM glucose (low O2: 58% vs high O2: 55%; Day 8). Our data suggest that low concentration of glucose during culture of bovine embryos is beneficial, and that generation of free oxygen radicals is partly caused by a high concentration of glucose in the medium.
authors.affiliation: Kobe City Horticulture Promoting Association, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: S
authors.initials: S
authors.lastname: Akamatsu
authors.forename: N
authors.initials: N
authors.lastname: Minami
authors.forename: M
authors.initials: M
authors.lastname: Yamada
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001755:Blastocyst; D002374:Catalase / Q000494:pharmacology; D002417:Cattle / Q000196:embryology*; D046508:Culture Techniques; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005947:Glucose / Q000008:administration & dosage*; D008353:Mannitol / Q000494:pharmacology; D010100:Oxygen / Q000008:administration & dosage; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008353:Mannitol; D002374:Catalase; D013482:Superoxide Dismutase; D005947:Glucose; D010100:Oxygen