Increased expression of endogenous biotin, but not BDNF, in telencephalic song regions during zebra finch vocal learning.
Brain-derived neurotrophic factor (BDNF) is thought to regulate multiple aspects of brain development and neural plasticity in vertebrates. We have examined BDNF expression in two telencephalic nuclei (RA and HVC) in the zebra finch brain that control song learning by juvenile males and the production of already-learned song by adults. Using two different antibody-labeling techniques (avidin-biotin complex and horseradish peroxidase), we were unable to detect BDNF-like immunoreactivity in RA of juvenile or adult birds. BDNF-like immunoreactive labeling of somata was detected in HVC, but the density of labeled cells was not different between juvenile and adult birds. Immunocytochemical findings were confirmed by RT-PCR for BDNF mRNA. Thus, in contrast to a previous report (Akutagawa and Konishi, Proc. Natl. Acad. Sci. USA 95 (1998) 11429-11434), we did not observe elevated levels of BDNF immunoreactivity in RA and HVC of juvenile birds that were learning to sing. However, RA and HVC of juvenile birds were found to express elevated levels of endogenous biotin (as detected by avidin peroxidase), suggesting a possible role for biotin-regulated mechanisms in songbird vocal learning.
Johnson
F
F
Department of Psychology and Program in Neuroscience, Florida State University, Tallahassee, FL, USA. johnson@psy.fsu.edu
Norstrom
E
E
Soderstrom
K
K
D000367:Age Factors; D000818:Animals; D000906:Antibodies; D001307:Auditory Perception / Q000502:physiology; D001710:Biotin / Q000096:biosynthesis*; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D004193:Discrimination Learning / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D008297:Male; D009473:Neuronal Plasticity / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012737:Sex Factors; D020308:Songbirds / Q000502:physiology*; D013687:Telencephalon / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D014828:Vocalization, Animal / Q000502:physiology*
D000906:Antibodies; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger; D001710:Biotin; D006735:Horseradish Peroxidase