Effect of hyaluronic acid on development of in vitro produced bovine embryos.
The present study was carried out to evaluate the effect of hyaluronic acid (HA) added to the culture medium on bovine embryo development to the blastocyst stage as well as embryo quality and viability after freezing and thawing. In vitro matured and fertilized (IVM/IVF) bovine oocytes from slaughterhouse ovaries were cultured for 8 d in SOFm supplemented with 4 mg/mL fatty acid-free BSA, either in the absence or presence of 1 or 0.5 mg/mL HA. There was a significant increase in blastocyst yield in the presence of 1 mg/mL HA (P < 0.01), whereas 0.5 mg/mL HA was ineffective. Cleavage rate and mean number of days to blastocyst formation were unaffected by HA at any concentration. At 1 mg/mL, HA did not affect either post-freeze survival of Grade 1 and 2 blastocysts or the number of nuclei per blastocyst. Supplementation with HA at 1 mg/mL also significantly enhanced embryo development up to the blastocyst stage (P < 0.05) in a chemically-defined culture medium without a protein source. It is concluded that supplementation of both semi-defined and defined culture media with 1 mg/mL HA improves the development of IVM/IVF bovine embryos to the blastocyst stage, without affecting embryo quality and post-freeze survival. These results open the possibility of including HA in culture media in order to increase the efficiency of in vitro blastocyst production from in vitro-matured bovine oocytes.
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Centro de Investigaciones Reproductivas Pérez Companc, Buenos Aires, Argentina. cirpc@datamarkets.com.ar
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Martínez
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D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry; D001755:Blastocyst / Q000502:physiology; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D015925:Cryopreservation / Q000662:veterinary; D003470:Culture Media; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005456:Fluorescent Dyes / Q000737:chemistry; D006820:Hyaluronic Acid / Q000494:pharmacology* / Q000502:physiology; D008297:Male; D008856:Microscopy, Fluorescence / Q000662:veterinary; D009865:Oocytes / Q000502:physiology
D001562:Benzimidazoles; D003470:Culture Media; D005456:Fluorescent Dyes; D006820:Hyaluronic Acid; C017807:bisbenzimide ethoxide trihydrochloride