title: The relationship of lymphocytes in blood and in lymph to sleep/wake states in sheep.
abstract: Based on evidence of a role for immune-associated cytokines in sleep induction, we investigated the possibility that lymphocyte distribution between blood and lymphatics could be altered as a function of sleep/wakefulness. Blood and lymph sample were obtained from 5 sheep during periods of slow-wave sleep and wake. Blood and lymph lymphocytes were phenotyped using monoclonal antibodies against CD4, CD8, gd T-cell receptors and a surface marker on ovine B cells. Lymph flow rates and efferent lymph cell output were measured. Lymph flow and prescapular efferent lymphocyte output were reduced during sleep compared to wakefulness (p<0.0005). There were no differences in lymphocyte subsets in the blood and in the lymph during sleep/wake brain states. These data indicate that migration of cells in the peripheral lymphatic system is altered during sleep compared to wakefulness.
authors.affiliation: Centre for Sleep and Chronobiology, Toronto Western Hospital, University of Toronto, Ontario, Canada.
authors.forename: J B
authors.initials: JB
authors.lastname: Dickstein
authors.forename: J B
authors.initials: JB
authors.lastname: Hay
authors.forename: F A
authors.initials: FA
authors.lastname: Lue
authors.forename: H
authors.initials: H
authors.lastname: Moldofsky
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002465:Cell Movement / Q000502:physiology; D016207:Cytokines / Q000097:blood; D005260:Female; D008196:Lymph / Q000378:metabolism*; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D012756:Sheep / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D016207:Cytokines