title: Relevance of commercial diagnostic tests to detection of enteric adenovirus infections in South Africa.
abstract: The prevalence of enteric adenoviruses detected by an in-house enzyme-linked immunosorbent assay (the RIVM-ELISA) ranged from 13 to 38%, and subgroup F adenoviruses comprised 86%. All subgroup F adenoviruses reacted with both RIVM anti-adenovirus type 40 (Ad40) and anti-adenovirus type 41 (Ad41) monoclonal antibodies but were not detected by Adenoclone Type 40/41 enzyme immunoassay (EIA). The correlation between the Biotrin EIA and RIVM-ELISA results was low (26%). Immunospecific tests suggest that a significant proportion of enteric adenoviruses, possibly comprising previously unidentified or emerging types, are not detected by commercial diagnostic tests in South Africa.
authors.affiliation: Department of Microbiology, University of the Witwatersrand, Johannesburg, South Africa. p.moore@rfc.ucl.ac.uk
authors.forename: P L
authors.initials: PL
authors.lastname: Moore
authors.forename: A D
authors.initials: AD
authors.lastname: Steele
authors.forename: J J
authors.initials: JJ
authors.lastname: Alexander
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005759:Gastroenteritis / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013019:South Africa / Q000453:epidemiology
chemical_list: D011933:Reagent Kits, Diagnostic