title: An open assay model to induce subclincal non-erythematous irritation.
abstract: To avoid the acute irritation and dryness that a single occlusive surfactant application (24-h patch test) may cause, and to approximate clinical use, an open application model was chosen to define subclinical non-erythematous irritation in the stratum corneum. This human test combined a supervised washing at the laboratory with at-home use of the test products by the subjects. Effects of washing with the surfactants on the dorsal hand and volar forearm were compared. The results demonstrated that this situational use model permits the exploration of subclinical surfactant-induced irritation. The forearm appeared to be more discriminative as compared to the dorsal hand. Squamometry proved to be a sensitive, complementary assessment method for detecting surfactant-induced subclinical skin surface alterations and for differentiating surfactant effects in this open assay.
authors.affiliation: Department of Dermatology, School of Medicine, University of California, San Francisco 94143-0989, USA.
authors.forename: V
authors.initials: V
authors.lastname: Charbonnier
authors.forename: B M
authors.initials: BM
authors.lastname: Morrison
authors.forename: M
authors.initials: M
authors.lastname: Paye
authors.forename: H I
authors.initials: HI
authors.lastname: Maibach
mesh_terms: D000328:Adult; D016001:Confidence Intervals; D017453:Dermatitis, Irritant / Q000175:diagnosis* / Q000209:etiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010328:Patch Tests / Q000379:methods*; D012016:Reference Values; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D012867:Skin / Q000737:chemistry / Q000473:pathology* / Q000503:physiopathology; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects / Q000031:analogs & derivatives*; D013501:Surface-Active Agents / Q000009:adverse effects
chemical_list: D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C408148:sodium laureth sulfate