Effect of exopolysaccharide V2-7, isolated from Halomonas eurihalina, on the proliferation in vitro of human peripheral blood lymphocytes.
The immunomodulatory activity of the exopolysaccharide V2-7, a sulfated polymer excreted by the moderately halophilic bacteria Halomonas eurihalina, was studied in vitro. [3H]thymidine incorporation and flow-cytometry measurements showed that this exopolysaccharide enhanced the unspecific proliferation of human lymphocytes in response to the presence of anti-CD3 monoclonal antibody. It was effective at concentrations of less than 1 microg/ml, maximum activity being achieved at 0.2 microg/ml.
Pérez-Fernández
M E
ME
Department of Microbiology, Faculty of Pharmacy, University of Granada, Spain.
Quesada
E
E
Gálvez
J
J
Ruiz
C
C
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D020632:Halomonas / Q000378:metabolism*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D016853:Muromonab-CD3 / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013936:Thymidine / Q000378:metabolism
D000276:Adjuvants, Immunologic; D007166:Immunosuppressive Agents; D016853:Muromonab-CD3; D011135:Polysaccharides, Bacterial; C411353:exopolysaccharide V2-7, Halomonas; D013936:Thymidine