title: A RIA combined with SPE for the determination of a dual D2-receptor and beta2-adrenoceptor agonist, AR-C68397XX, in human plasma.
abstract: A radioimmunoassay has been developed for the determination of AR-C68397XX, a dual D2-receptor and beta2-adrenoceptor agonist, in human plasma. The method incorporates solid phase sample extraction and is suitable for the determination of the analyte at pg ml(-1) concentrations. The antiserum was raised in Suffolk cross sheep following primary and booster immunisations with an immunogen prepared by conjugating a carboxyphenylmethyl derivative of AR-C68397XX, to bovine serum albumin. The radioligand was prepared by the 125I-labelled iodination of a derivative of AR-C68397XX. The solid phase extraction procedure, using octadecyl sorbent, was introduced to remove matrix interferences in the plasma and to enhance method sensitivity. The calibration range is 20-500 pg ml(-1), using 0.5 ml of undiluted human plasma sample.
authors.affiliation: Department of Physical and Metabolic Sciences, AstraZeneca R&D Charnwood, Loughborough, Leics., UK. viv.willson@charnwood.ab.astra.com
authors.forename: V J
authors.initials: VJ
authors.lastname: Willson
authors.forename: J R
authors.initials: JR
authors.lastname: Preston
authors.forename: W J
authors.initials: WJ
authors.lastname: Lockley
authors.forename: A
authors.initials: A
authors.lastname: Mather
authors.forename: J
authors.initials: J
authors.lastname: Singh
authors.forename: D
authors.initials: D
authors.lastname: Wilkinson
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000097:blood* / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D018491:Dopamine Agonists / Q000097:blood* / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes / Q000032:analysis; D007553:Isotope Labeling; D019999:Pharmaceutical Solutions; D011863:Radioimmunoassay; D017448:Receptors, Dopamine D2 / Q000819:agonists*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012756:Sheep / Q000276:immunology; D013844:Thiazoles / Q000097:blood* / Q000276:immunology
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; D018491:Dopamine Agonists; D007457:Iodine Radioisotopes; D019999:Pharmaceutical Solutions; D017448:Receptors, Dopamine D2; D013844:Thiazoles; D012710:Serum Albumin, Bovine; C116267:sibenadet