title: Estimation of nonheme-iron bioavailability from meal composition.
abstract: BACKGROUND
  Considerable data are available on the individual effects of dietary factors on nonheme-iron absorption, but their combined effect when they are present in the same meal is not known.
  
  
  OBJECTIVE
  Our objective was to predict the bioavailability of iron from complex meals that are consumed commonly in the United States on the basis of the contents of factors that are known to promote or inhibit food iron absorption.
  
  
  METHODS
  Radioisotopic measurements of nonheme-iron absorption from 25 meals were made in 86 volunteer subjects by using extrinsic radioiron labeling. The meal contents of nonheme iron, calcium, ascorbic acid, polyphenols, and phytic acid were determined by biochemical analysis; energy and protein contents were estimated from food-composition tables. Animal tissue content was based on weight or was obtained from the manufacturer.
  
  
  RESULTS
  After adjusting iron absorption for differences in iron status, the significant biochemical predictors of iron absorption as determined by multiple regression analysis were the contents of animal tissue (P = 0.0001), phytic acid (P = 0.0001), and ascorbic acid (P = 0. 0441). Collectively, these 3 variables accounted for 16.4% of the variation in absorption. On the basis of the multiple regression analysis, we developed the following equation to estimate iron absorption: Ln absorption, % (adjusted to serum ferritin concentration of 30 microg/L) = 1.9786 + (0.0123 x animal tissue in g) - (0.0034 x phytic acid in mg) + (0.0065 x ascorbic acid in mg).
  
  
  CONCLUSIONS
  For the 25 meals evaluated, only the contents of animal tissue, phytic acid, and ascorbic acid were useful for estimating nonheme-iron absorption.
authors.affiliation: Department of Medicine, Kansas University Medical Center, Kansas City, USA. mbreddy@iastate.edu
authors.forename: M B
authors.initials: MB
authors.lastname: Reddy
authors.forename: R F
authors.initials: RF
authors.lastname: Hurrell
authors.forename: J D
authors.initials: JD
authors.lastname: Cook
mesh_terms: D000328:Adult; D001205:Ascorbic Acid / Q000008:administration & dosage; D001682:Biological Availability; D002136:Calcium, Dietary / Q000008:administration & dosage; D004032:Diet; D004044:Dietary Proteins / Q000008:administration & dosage; D002149:Energy Intake; D005260:Female; D005419:Flavonoids; D005502:Food; D006801:Humans; D007408:Intestinal Absorption; D007504:Iron Radioisotopes; D019266:Iron, Dietary / Q000493:pharmacokinetics*; D008297:Male; D008460:Meat; D010636:Phenols / Q000008:administration & dosage; D010833:Phytic Acid / Q000008:administration & dosage; D011108:Polymers / Q000008:administration & dosage; D059808:Polyphenols; D012044:Regression Analysis
chemical_list: D002136:Calcium, Dietary; D004044:Dietary Proteins; D005419:Flavonoids; D007504:Iron Radioisotopes; D019266:Iron, Dietary; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D010833:Phytic Acid; D001205:Ascorbic Acid