title: Selection of a potent inhibitor of trihydroxynaphthalene reductase by sorting disease control data.
abstract: Compounds that control rice blast, but not other crop diseases, were selected for testing as inhibitors of trihydroxynaphthalene reductase of the fungal melanin biosynthetic pathway. A potent inhibitor of the enzyme (2) (Ki = 25 nM) was identified. An X-ray structure of the enzyme-NADPH-2 complex was determined at 2.1 A resolution.
authors.affiliation: DuPont Central Research and Development, Experimental Station, Wilmington DE, 19880, USA.
authors.forename: D I
authors.initials: DI
authors.lastname: Liao
authors.forename: G S
authors.initials: GS
authors.lastname: Basarab
authors.forename: A A
authors.initials: AA
authors.lastname: Gatenby
authors.forename: D B
authors.initials: DB
authors.lastname: Jordan
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D016208:Databases, Factual; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005656:Fungal Proteins; D005658:Fungi / Q000187:drug effects*; D008543:Melanins / Q000096:biosynthesis; D008968:Molecular Conformation; D009249:NADP / Q000737:chemistry; D012275:Oryza; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010935:Plant Diseases / Q000382:microbiology*; D014961:X-Ray Diffraction
chemical_list: D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D008543:Melanins; D009249:NADP; D010088:Oxidoreductases; C086024:tetrahydroxynaphthalene reductase; D044925:Oxidoreductases Acting on CH-CH Group Donors