Pathogenic role of SEF14, SEF17, and SEF21 fimbriae in Salmonella enterica serovar enteritidis infection of chickens.
Very little is known about the contribution of surface appendages of Salmonella enterica serovar Enteritidis to pathogenesis in chickens. This study was designed to clarify the role of SEF14, SEF17, and SEF21 fimbriae in serovar Enteritidis pathogenesis. Stable, single, defined sefA (SEF14), agfA (SEF17), and fimA (SEF21) insertionally inactivated fimbrial gene mutants of serovar Enteritidis were constructed. All mutant strains invaded Caco-2 and HT-29 enterocytes at levels similar to that of the wild type. Both mutant and wild-type strains were ingested equally well by chicken macrophage cell lines HD11 and MQ-NCSU. There were no significant differences in the abilities of these strains to colonize chicken ceca. The SEF14(-) strain was isolated in lower numbers from the livers of infected chickens and was cleared from the spleens faster than other strains. No significant differences in fecal shedding of these strains were observed.
Rajashekara
G
G
Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota 55108, USA.
Munir
S
S
Alexeyev
M F
MF
Halvorson
D A
DA
Wells
C L
CL
Nagaraja
K V
KV
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018938:Caco-2 Cells; D002460:Cell Line; D002645:Chickens / Q000382:microbiology; D003470:Culture Media; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D019073:HT29 Cells; D006801:Humans; D008264:Macrophages / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012477:Salmonella enteritidis / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence
C107306:AgfA protein, Salmonella enterica; D001426:Bacterial Proteins; D003470:Culture Media; C063055:fimbrillin; C080931:sefA protein, Salmonella enteritidis; D036781:Fimbriae Proteins