A simple and rapid method for the preparation of a cell-free extract with CCAAT-binding activity from filamentous fungi.
A simple and rapid method for the preparation of a cell-free extract with the CCAAT-binding activity was established with Aspergillus nidulans as a model fungus. Proteins were extracted with 6 M guanidine hydrochloride directly from mycelia and renatured by dialysis. This method was found applicable to other filamentous fungi such as Aspergillus oryzae and Trichoderma viride.
Kato
M
M
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Japan. kato@agr.nagoya-u.ac.jp
Hayashi
K
K
Kobayashi
T
T
Tsukagoshi
N
N
D001230:Aspergillus / Q000378:metabolism*; D022762:CCAAT-Enhancer-Binding Proteins; D002474:Cell-Free System; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004586:Electrophoresis; D005656:Fungal Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D014242:Trichoderma / Q000378:metabolism*
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins