Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F.
Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (CsCl-EtBr) density gradient ultracentrifugation. Agarose gel electrophoresis of plasmid DNA and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Pediococcus acidilactici F. Plasmid encoding bacteriocin production in P. acidilactici F was examined for restriction enzyme cleavage patterns and its map has been constructed. An Escherichia coli strain transformed with the recombinant plasmid, pQE322, produced and, most probably, secreted pediocin F.
Osmanağaoğlu
O
O
Animal Science Department, University of Wyoming, Laramie 82071, USA. ozlemos@quark.fev.gazi.edu.tr
Beyatli
Y
Y
Gündüz
U
U
D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005516:Food Microbiology; D008461:Meat Products / Q000382:microbiology; D010376:Pediococcus / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis
D001430:Bacteriocins; D011994:Recombinant Proteins