Oligobodies: bench made synthetic antibodies.
Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA sequence from a database provides enough information to make them.
Radrizzani
M
M
Instituto de Investigaciones Bioquímicas-Fundación Campomar, Buenos Aires, Argentina.
Broccardo
M
M
González Solveyra
C
C
Bianchini
M
M
Reyes
G B
GB
Cafferata
E G
EG
Santa-Coloma
T A
TA
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000276:immunology; D019151:Peptide Library; D010749:Phosphoprotein Phosphatases / Q000276:immunology; D016133:Polymerase Chain Reaction; D054648:Protein Phosphatase 2; D011817:Rabbits; D017422:Sequence Analysis, DNA
D009841:Oligonucleotides; D019151:Peptide Library; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2