The formation of heterodimers by vancomycin group antibiotics.
The formation of heterodimers in mixtures of glycopeptide antibiotics has been detected by electrospray ionization mass spectrometry (ESI-MS), and dimerization constants have been determined. By using NMR spectroscopy, it has been shown that these heterodimers indeed exist in aqueous solution. The dimerization constants obtained by NMR spectroscopy are in good agreement with those determined by ESI-MS. Structural information on the heterodimer interface of some of the heterodimers is obtained by using two-dimensional NMR techniques and reveals that these heterodimers are similar in structure to the homodimers.
Staroske
T
T
Dominic Cambridge Centre for Molecular Recognition, University Chemical Laboratory, Cambridge, UK.
O'Brien
D P
DP
Jørgensen
T J
TJ
Roepstorff
P
P
Williams
D H
DH
Heck
A J
AJ
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D019281:Dimerization; D006020:Glycopeptides; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008956:Models, Chemical; D008958:Models, Molecular; D012310:Ristocetin / Q000378:metabolism; D018629:Spectrometry, Mass, Secondary Ion; D014640:Vancomycin / Q000031:analogs & derivatives / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; C112142:chloroeremomycin; C053759:eremomycin; D012310:Ristocetin; C016719:ristocetin A; D014640:Vancomycin