title: Modification of disaccharidase activity measurement in rat bowel.
abstract: This study describes a modification of the existing disaccharidase assay in rat small bowel in which whole bowel, rather than mucosa, is utilized. In addition, the use of total vs. specific activity as a more accurate unit of measurement of disaccharidase activity is discussed.
authors.affiliation: Department of Surgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, USA.
authors.forename: J
authors.initials: J
authors.lastname: Arcuni
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Franson
authors.forename: R E
authors.initials: RE
authors.lastname: Sonnino
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004186:Disaccharidases / Q000032:analysis / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000502:physiology; D007421:Intestine, Small / Q000201:enzymology* / Q000502:physiology; D043322:Lactase; D008297:Male; D009130:Muscle, Smooth / Q000201:enzymology / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D015203:Reproducibility of Results; D013393:Sucrase / Q000032:analysis / Q000378:metabolism; D000520:alpha-Glucosidases / Q000032:analysis / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004186:Disaccharidases; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase