title: The Yersinia enterocolitica phospholipase gene yplA is part of the flagellar regulon.
abstract: Yersinia enterocolitica yplA encodes a phospholipase required for virulence. Virulence genes are often regulated in response to environmental signals; therefore, yplA expression was examined using a yplA::lacZY transcriptional fusion. Maximal yplA expression occurred between pH 6.5 and pH 7.5 and was induced in the mid-logarithmic growth phase. Potential Fnr, cyclic AMP (cAMP)-cAMP receptor protein (Crp), and sigma(F) regulatory sites were identified in the nucleotide sequence. Reduction of yplA expression by aeration, addition of glucose and sucrose, and application of high temperature and salt is consistent with Fnr-, cAMP-Crp-, and sigma(F)-mediated regulation, respectively. Expression of yplA was reduced in flhDC and fliA null strains, indicating that yplA is part of the flagellar regulon.
authors.affiliation: Department of Molecular Microbiology, Department of Pediatrics, Washington University School of Medicine, Missouri 63110, USA.
authors.forename: D H
authors.initials: DH
authors.lastname: Schmiel
authors.forename: G M
authors.initials: GM
authors.lastname: Young
authors.forename: V L
authors.initials: VL
authors.lastname: Miller
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D029968:Escherichia coli Proteins; D005407:Flagella / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D010740:Phospholipases / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D018085:Regulon; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D014774:Virulence / Q000235:genetics; D015008:Yersinia enterocolitica / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C064971:FliA protein, Bacteria; D012808:Sigma Factor; D015534:Trans-Activators; C090552:flhC protein, E coli; C090553:flhD protein, E coli; D010740:Phospholipases