title: Glucose uptake increases relative to oxygen consumption during short-term hibernation.
abstract: BACKGROUND
  Although glucose uptake is increased in chronically hypoperfused, viable myocardium, the dynamic changes in glucose uptake relative to oxygen consumption in "short-term" models of hibernation have not been fully explored.
  
  
  METHODS
  14 anesthetized swine were instrumented with an hydraulic occluder and flow probe on the proximal LAD artery. Blood flow was reduced approximately 30% for 1 hour. Myocardial blood flow and uptake of oxygen, free fatty acids, glucose and lactate were determined in the LAD region at baseline and at 10, 30, and 60 minutes of ischemia. Transmural biopsies for ATP and creatine phosphate (CP) were obtained in the LAD region prior to and at 15 and 45 minutes of ischemia. In 5 animals, glycogen was assayed at baseline and at the end of 60 minutes of ischemia.
  
  
  RESULTS
  In the LAD region, myocardial oxygen consumption was reduced from 2.06 +/- 0.16 micromol/min/gram to 1.46 +/- 0.13 micromol/min/gram (P < 0.05). By 15 minutes of ischemia, transmural creatine phosphate fell from 7.48 +/- 0.76 micromol/g-wet weight at baseline to 6.19 +/- 0.32 micromol/g-wet weight (P < 0.05) but normalized by 45 minutes of ischemia (7.39 +/- 0.56 micromol/g-wet weight; NS). Between 10 and 60 minutes of constant flow reduction, glucose uptake as a percentage of MVO2 increased from 3 +/- 2% to 10 +/- 2% (P < 0.05) while lactate uptake increased from -9 +/- 9% to -1 +/- 2% (P < 0.05). Glycogen decreased from 27.8 +/- 3.7 at baseline to 16.9 +/- 1.2 micromol/g-wet weight at end-ischemia.
  
  
  CONCLUSIONS
  In this model of short-term hibernation, glucose and lactate uptake increase relative to oxygen consumption during sustained ischemia, and temporally coincide with the recovery of bioenergetics. The findings are consistent with the notion that glycolytically derived ATP is important for the maintainance of energy supply during sustained ischemia.
authors.affiliation: Cardiology and Cardiovascular Surgery, VA Medical Center, Minneapolis, MN 55417, USA. mcfal1001@maroon.tc.umn.edu
authors.forename: E O
authors.initials: EO
authors.lastname: McFalls
authors.forename: D R
authors.initials: DR
authors.lastname: Baldwin
authors.forename: D
authors.initials: D
authors.lastname: Marx
authors.forename: K
authors.initials: K
authors.lastname: Maxwell
authors.forename: H B
authors.initials: HB
authors.lastname: Ward
mesh_terms: D000818:Animals; D004734:Energy Metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006605:Hibernation / Q000502:physiology*; D008297:Male; D010101:Oxygen Consumption; D013552:Swine; D013997:Time Factors
chemical_list: D005947:Glucose