Reversed-phase high performance liquid chromatographic separation of the enantiomers of terbutaline by derivatization with 2,3,4,6-tetra-o-acetyl-beta-D-glucopyranosyl isothiocyanate.
The enantiomers of the bronchodilator terbutaline were separated by reversed-phase high performance liquid chromatography after derivatization with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate(GITC) reagent. The derivatization proceeded quantitatively within 1 h at room temperature. The corresponding diastereomeric thiourea derivatives were well resolved on an ODS column with acetonitrile-acetate buffer as a mobile phase. Elution orders of the diastereomers were confirmed by derivatization of R-(-)-terbutaline and S-(+)-terbutaline which were collected by semi-preparative chiral HPLC using Sumichiral OA-4700 column. The native fluorescence of terbutaline was quenched by derivatization with GITC. The detection limit was 25 ng when monitored at UV 278 nm.
Kim
K H
KH
College of Pharmacy, Kangwon National University, Chunchon, Korea. kyeong@cc.kangwon.ac.kr
Kim
D S
DS
Hong
S P
SP
Keon
O S
OS
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D017879:Isothiocyanates; D013237:Stereoisomerism; D013726:Terbutaline / Q000032:analysis*
D000318:Adrenergic beta-Agonists; D017879:Isothiocyanates; C041943:2,3,4,6-tetra-O-acetylglucopyranosylisothiocyanate; D013726:Terbutaline