title: The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica.
abstract: The structure was solved at 2.5 A resolution using multiwavelength anomalous dispersion (MAD) scattering by Se-Met residues. The subunit of N(10)-formyltetrahydrofolate synthetase is composed of three domains organized around three mixed beta-sheets. There are two cavities between adjacent domains. One of them was identified as the nucleotide binding site by homology modeling. The large domain contains a seven-stranded beta-sheet surrounded by helices on both sides. The second domain contains a five-stranded beta-sheet with two alpha-helices packed on one side while the other two are a wall of the active site cavity. The third domain contains a four-stranded beta-sheet forming a half-barrel. The concave side is covered by two helices while the convex side is another wall of the large cavity. Arg 97 is likely involved in formyl phosphate binding. The tetrameric molecule is relatively flat with the shape of the letter X, and the active sites are located at the end of the subunits far from the subunit interface.
authors.affiliation: Department of Chemistry, University of South Carolina, Columbia, South Carolina 29208, USA.
authors.forename: R
authors.initials: R
authors.lastname: Radfar
authors.forename: R
authors.initials: R
authors.lastname: Shin
authors.forename: G M
authors.initials: GM
authors.lastname: Sheldrick
authors.forename: W
authors.initials: W
authors.lastname: Minor
authors.forename: C R
authors.initials: CR
authors.lastname: Lovell
authors.forename: J D
authors.initials: JD
authors.lastname: Odom
authors.forename: R B
authors.initials: RB
authors.lastname: Dunlap
authors.forename: L
authors.initials: L
authors.lastname: Lebioda
mesh_terms: D000595:Amino Acid Sequence; D003013:Clostridium / Q000737:chemistry / Q000201:enzymology*; D003460:Crystallization; D005574:Formate-Tetrahydrofolate Ligase / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment
chemical_list: D005574:Formate-Tetrahydrofolate Ligase