Polymyxin B binds to anandamide and inhibits its cytotoxic effect.
Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to be a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol. We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock.
Wang
Y
Y
Department of Laboratory and Molecular Medicine, Faculty of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, Japan.
Liu
Y
Y
Sarker
K P
KP
Nakashima
M
M
Serizawa
T
T
Kishida
A
A
Akashi
M
M
Nakata
M
M
Kitajima
I
I
Maruyama
I
I
D000327:Adsorption / Q000187:drug effects; D000818:Animals; D001095:Arachidonic Acids / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D001798:Blood Proteins / Q000494:pharmacology; D002186:Cannabinoids / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D063388:Endocannabinoids; D006801:Humans; D007022:Hypotension / Q000139:chemically induced / Q000188:drug therapy; D008070:Lipopolysaccharides / Q000633:toxicity; D008863:Microspheres; D016716:PC12 Cells; D011112:Polymyxin B / Q000378:metabolism* / Q000494:pharmacology* / Q000627:therapeutic use; D053284:Polyunsaturated Alkamides; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D012772:Shock, Septic / Q000139:chemically induced / Q000188:drug therapy; D020349:Surface Plasmon Resonance
D001095:Arachidonic Acids; D001798:Blood Proteins; D002186:Cannabinoids; D063388:Endocannabinoids; D008070:Lipopolysaccharides; D053284:Polyunsaturated Alkamides; D011112:Polymyxin B; C078814:anandamide