title: A new specialized cell-matrix interaction in actively resorbing osteoclasts.
abstract: We have identified a novel cell-matrix interaction in activated osteoclasts. Resorbing osteoclasts maintain a barrier adjacent to the bone surface that prevents the leakage of secreted protons and proteases from the resorption area. Using a series of fluorescent dyes of known molecular mass and different surface charge we established that negatively charged molecules with M(r )up to 10,000 rapidly accumulate underneath actively resorbing osteoclasts. Live cell imaging shows that staining could be detected underneath the osteoclasts as early as 30 seconds after the addition of the low molecular mass markers. We provide evidence that the actin cytoskeleton and the adhesion substrate in contact with the cells are critically involved in the maintenance of the sealing barrier. These data taken together suggest that the accumulation under resorbing osteoclasts is by diffusion rather than transcytotic delivery. Our results indicate that the net concentration of secreted and resorbed components is a balance between generation rate and limited diffusion rather than the presence of an impermeable barrier as previously suggested. This dynamic osteoclast sealing zone may, thus, provide the mechanism by which osteoclast migration and resorption can occur simultaneously.
authors.affiliation: Bone and Mineral Centre, University College London, London WC1E 6JJ, UK. g.stenbeck@ucl.ac.uk
authors.forename: G
authors.initials: G
authors.lastname: Stenbeck
authors.forename: M A
authors.initials: MA
authors.lastname: Horton
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001862:Bone Resorption; D056657:Cathepsin K; D002403:Cathepsins / Q000378:metabolism; D002448:Cell Adhesion; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007546:Isoquinolines / Q000378:metabolism; D018942:Macrolides; D008970:Molecular Weight; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D011817:Rabbits; D025262:Vacuolar Proton-Translocating ATPases
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007546:Isoquinolines; D018942:Macrolides; C057620:bafilomycin A; C017475:lucifer yellow; D002403:Cathepsins; D056657:Cathepsin K; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases