title: Spatial and temporal changes in natural and target deprivation-induced cell death in the mouse inferior olive.
abstract: The survival of inferior olive neurons is dependent on contact with cerebellar Purkinje cells. There is evidence that this dependence changes with time. Because inferior olivary axons, called climbing fibers, already show significant topographical ordering in cerebellar target zones during late embryogenesis in mice, the question arises as to whether olive neurons are dependent on target Purkinje cells for their survival at this early age. To better characterize this issue, inferior olive development was studied in two transgenic mouse mutants, wnt-1 and L7ADT, with embryonic and early postnatal loss of cerebellar target cells, respectively, and compared to that in the well-studied mutant, Lurcher. Morphological criteria as well as quantitative measures of apoptosis were considered in this developmental analysis. Survival of inferior olive neurons is observed to be independent of Purkinje cells throughout embryogenesis, but dependence begins immediately at birth in both wild types and mutants. Thereafter, wild types and mutants show a rapid increase in olive cell apoptosis, with a peak at postnatal day 4, followed by a period of low-level, but significant, apoptosis that continues to at least postnatal day 11; the main difference is that apoptosis is quantitatively enhanced in the mutants compared to wild types. The multiphasic course of these effects roughly parallels the known phases of climbing fiber synaptogenesis. In addition, despite significant temporal differences among the mutants with respect to absolute numbers of dying cells, there are common spatial features suggestive of distinct intrinsic programs linking different olivary subnuclei to their targets.
authors.affiliation: Department of Neuroscience and the Neurobiotechnology Center, The Ohio State University College of Medicine, Columbus, Ohio, USA.
authors.forename: T
authors.initials: T
authors.lastname: Chu
authors.forename: H
authors.initials: H
authors.lastname: Hullinger
authors.forename: K
authors.initials: K
authors.lastname: Schilling
authors.forename: J
authors.initials: J
authors.lastname: Oberdick
mesh_terms: D000818:Animals; D017209:Apoptosis; D002450:Cell Communication; D002454:Cell Differentiation; D002470:Cell Survival; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009847:Olivary Nucleus / Q000166:cytology / Q000378:metabolism*; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism; D013997:Time Factors