Role of Atf1 and Pap1 in the induction of the catalase gene of fission yeast schizosaccharomyces pombe.
We examined the induction of the catalase gene (ctt1(+)) of fission yeast Schizosaccharomyces pombe in response to several stresses by using mutants of transcription factors (Atf1 and Pap1) and a series of deletion mutants of the ctt1(+) promoter region. A transcription factor, Atf1, and its binding site are necessary for the induction of ctt1(+) by osmotic stress, UV irradiation, and heat shock. Induction by menadione treatment, which produces superoxide anion, required element A, the region from -111 to -90 (numbered with the transcription start site as +1). The factor responsible for the induction of the gene by oxidative stress via element A was identified as the transcription factor Pap1. We also found that Atf1 is activated by menadione treatment in pap1 mutant cells, although it is not activated by menadione treatment in pap1(+) cells. The activity of catalase is not increased in pap1 cells by several stresses, despite mRNA induction, suggesting that Pap1 plays some role in the expression of catalase activity.
Nakagawa
C W
CW
Department of Genetics, Institute for Developmental Research, Kagiya-cho, Kasugai, Aichi 480-0392, Japan. normutoh@inst-hsc.pref. aichi.jp
Yamada
K
K
Mutoh
N
N
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D051697:Activating Transcription Factor 1; D000222:Adaptation, Physiological / Q000235:genetics; D050976:Basic-Leucine Zipper Transcription Factors; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal; D009997:Osmotic Pressure; D018384:Oxidative Stress; D000073718:Pancreatitis-Associated Proteins; D010750:Phosphoproteins; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D051697:Activating Transcription Factor 1; D050976:Basic-Leucine Zipper Transcription Factors; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; D010750:Phosphoproteins; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C106740:atf1 protein, S pombe; D002374:Catalase; D000123:Acetyltransferases