Establishment and epitope analysis of allo-specific cytotoxic T lymphocytes at a tumor site recognizing a spouse's HLA-A0206 molecule.
OBJECTIVE
The molecular basis of allo-reactivity in reproductive immunity has not been fully clarified.


METHODS
Cytotoxic T lymphocytes (CTLs) were established from tumor-infiltrating lymphocytes (TILs). The allo-reactivity of the CTLs against various tumor cell lines or human leukocyte antigen (HLA)-A allele-transfected COS-7 cells was measured by 51Cr-release or interferon-gamma production assay.


CONCLUSIONS
We have established CTLs reacting to an HLA-A0206 molecule that matched a spouse's HLA-A allele from the TILs of a 68-year-old multiparous patient with gastric cancer. The amino acids at positions 66 and 88 in the alpha1 domain of HLA-A0206, both of which were common in the other HLA-A2 subtypes, were involved in the recognition by the CTLs. Endogenous peptides in the groove were not involved in the recognition. These results suggest the presence of long-lasting memory CTLs raised by the reproduction process, and may facilitate a better understanding of the molecular basis of allo-recognition during reproduction.
Seki
N
N
Department of Immunology, Kurume University School of Medicine, Japan.
Yamada
A
A
Suefuji
Y
Y
Mine
T
T
Tanaka
S
S
Gomi
S Y
SY
Kawagoe
N
N
Koufuji
K
K
Itoh
K
K
D000368:Aged; D000483:Alleles; D000818:Animals; D019556:COS Cells; D018984:Epitopes, T-Lymphocyte / Q000032:analysis*; D005260:Female; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007156:Immunologic Memory; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008297:Male; D018454:Spouses; D013274:Stomach Neoplasms / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D018984:Epitopes, T-Lymphocyte; D015789:HLA-A2 Antigen