Considerations on photochemical genotoxicity: report of the International Workshop on Genotoxicity Test Procedures Working Group.
Recent toxicological observations have caused concern regarding the need to test, for example, pharmaceuticals and cosmetic products for photochemical genotoxicity. The objective of this report is to give assistance on how to adapt existing test methods to investigate the potential of light-absorbing compounds to induce genotoxic effects on photoactivation. In general, the Organization for Economic Co-Operation & Economic Development (OECD) draft guideline on in vitro phototoxicity testing served as a basis for consideration. Concomitant exposure of the cells to the test compound and solar simulated light was considered appropriate as the initial, basic test condition. Optimization of the exposure scheme, e.g., a change of the irradiation spectrum, might be indicated depending on the initial test results. Selection of test compound concentrations should be based on results obtained with the dark version of the respective test system but might have to be modified if phototoxic effects are observed. Selection of the irradiation dose has to be performed individually for each test system based on dose-effect studies. The irradiation should induce per se a small, reproducible toxic or genotoxic effect. The report includes a specification of necessary controls, discusses factors that might have an impact on the irradiation characteristics, and gives a rationale for the omission of an external metabolic activation system. It also addresses the question that physicochemical and pharmacokinetic properties might trigger the need to test a chemical for photochemical genotoxicity. Relevant experimental observations are presented to back up the recommendations. The working group did not reach a consensus as to whether a single, adequately perfomed in vitro test for clastogenicity would be sufficient to exclude a photogenotoxic liability or whether a test battery including a gene mutation assay would be needed for product safety testing regarding photochemical genotoxicity.
Gocke
E
E
F. Hoffmann-La Roche Ltd., Basel, Switzerland. elmar.gocke@roche.com
Müller
L
L
Guzzie
P J
PJ
Brendler-Schwaab
S
S
Bulera
S
S
Chignell
C F
CF
Henderson
L M
LM
Jacobs
A
A
Murli
H
H
Snyder
R D
RD
Tanaka
N
N
D000818:Animals; D002460:Cell Line; D004307:Dose-Response Relationship, Radiation; D017408:Guidelines as Topic; D009152:Mutagenicity Tests; D010777:Photochemistry; D014466:Ultraviolet Rays