title: Structure and function of mutationally generated monomers of dimeric phosphoribosylanthranilate isomerase from Thermotoga maritima.
abstract: BACKGROUND
  Oligomeric proteins may have been selected for in hyperthermophiles because subunit association provides extra stabilization. Phosphoribosylanthranilate isomerase (PRAI) is monomeric and labile in most mesophilic microorganisms, but dimeric and stable in the hyperthermophile Thermotoga maritima (tPRAI). The two subunits of tPRAI are associated back-to-back and are locked together by a hydrophobic loop. The hypothesis that dimerization is important for thermostability has been tested by rationally designing monomeric variants of tPRAI.
  
  
  RESULTS
  The comparison of tPRAI and PRAI from Escherichia coli (ePRAI) suggested that levelling the nonplanar dimer interface would weaken the association. The deletion of two residues in the loop loosened the dimer. Subsequent filling of the adjacent pocket and the exchange of polar for apolar residues yielded a weakly associating and a nonassociating monomeric variant. Both variants are as active as the parental dimer but far more thermolabile. The thermostability of the weakly associating monomer increased significantly with increasing protein concentration. The X-ray structure of the nonassociating monomer differed from that of the parental subunit only in the restructured interface. The orientation of the original subunits was maintained in a crystal contact between two monomers.
  
  
  CONCLUSIONS
  tPRAI is dimeric for reasons of stability. The clearly separated responsibilities of the betaalpha loops, which are involved in activity, and the alphabeta loops, which are involved in protein stability, has permitted the evolution of dimers without compromising their activity. The preserved interaction in the crystal contacts suggests the most likely model for dimer evolution.
authors.affiliation: Abteilung für Biophysikalische Chemie, Biozentrum der Universität Basel, Basel, CH-4056, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Thoma
authors.forename: M
authors.initials: M
authors.lastname: Hennig
authors.forename: R
authors.initials: R
authors.lastname: Sterner
authors.forename: K
authors.initials: K
authors.lastname: Kirschner
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D018360:Crystallography, X-Ray; D017931:DNA Primers; D019281:Dimerization; D019143:Evolution, Molecular; D008958:Models, Molecular; D013329:Structure-Activity Relationship; D020124:Thermotoga maritima / Q000201:enzymology*
chemical_list: D017931:DNA Primers; D019747:Aldose-Ketose Isomerases; C029865:phosphoribosylanthranilate isomerase