title: Intracellular trafficking and release of intact edible mushroom lectin from HT29 human colon cancer cells.
abstract: Our previous studies have shown that the Galbeta1-3GalNAcalpha- (Thomsen-Friedenreich antigen)-binding lectin from the common edible mushroom Agaricus bisporus (ABL) reversibly inhibits cell proliferation, and this effect is a consequence of inhibition of nuclear localization sequence-dependent nuclear protein import after ABL internalization [Yu, L.G., Fernig, D.G., White, M.R.H., Spiller, D.G., Appleton, P., Evans, R.C., Grierson, I., Smith, J.A., Davies, H., Gerasimenko, O.V., Petersen, O.H., Milton, J.D. & Rhodes, J.M. (1999) J. Biol. Chem. 274, 4890-4899]. Here, we have investigated further the intracellular trafficking and fate of ABL after internalization in HT29 human colon cancer cells. Internalization of 125I-ABL occurred within 30 min of the lectin being bound to the cell surface. Subcellular fractionation after pulse labelling of the cells with 125I-ABL for 2 h at 4 degrees C followed by culture of the cells at 37 degrees C demonstrated a steady increase in radioactivity in a crude nuclear extract. The radioactivity in this extract reached a maximum after 10 h and declined after 20 h. Release of ABL from the cell, after pulse labelling, was assessed using both fluorescein isothiocyanate-labelled ABL and 125I-ABL and was slow, with a t1/2 of 48 h. Most of the 125I-ABL both inside cells and in the medium remained intact, as determined by trichloroacetic acid precipitation and SDS/PAGE, and after 48 h only 22 +/- 2% of ABL in the medium and 14 +/- 2% inside the cells was degraded. This study suggests that the reversibility of the antiproliferative effect of ABL is associated with its release from cells after internalization. The internalization and subsequent slow release, with little degradation of ABL, reflects the tendency of lectins to resist biodegradation and implies that other endogenous or exogenous lectins may be processed in this way by intestinal epithelial cells.
authors.affiliation: Department of Medicine and School of Biological Sciences, University of Liverpool, Liverpool L69 3GA, UK.
authors.forename: L G
authors.initials: LG
authors.lastname: Yu
authors.forename: D G
authors.initials: DG
authors.lastname: Fernig
authors.forename: J M
authors.initials: JM
authors.lastname: Rhodes
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D001692:Biological Transport; D019073:HT29 Cells; D006801:Humans; D007457:Iodine Radioisotopes; D037102:Lectins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: C041433:Agaricus lectins; D007457:Iodine Radioisotopes; D037102:Lectins