title: Quantitative determination of total coliforms and Escherichia coli in marine waters with chromogenic and fluorogenic media.
abstract: This study compared the performance of LMX(R) broth (LMX), Chromocult Coliform(R) agar (CC) and Chromocult Coliform agar plus cefsulodin (10 microg ml-1) (CC-CFS), with standard methods multiple tube fermentation (MTF), for the enumeration of total coliforms and Escherichia coli from marine recreational waters. LMX and CC are two media designed to concurrently detect total coliform (TC) bacteria and E. coli by the specific action of beta-galactosidase (total coliforms) and beta-glucuronidase (E. coli). Overall results for the TC test showed that LMX, CC and MTF recovered 2.63, 1.95 and 1.90 times as many TCs as CC-CFS, respectively. Data from the multiple range test showed significant differences (P < 0.05) between TC counts on CC-CFS and LMX. The traditional MTF was less sensitive for E. coli enumeration. However, there was no statistically significant differences between LMX, CC, CC-CFS and the MTF method for E. coli enumeration. Background interference was reduced on CC-CFS and the counts obtained reflected more accurately the number of TCs. Therefore, the contribution of beta-galactosidase positive, non coliform bacteria (Aeromonas spp. and Vibrio spp.) to TC counts should not be neglected.
authors.affiliation: Hygiene Institute, University of Vienna, Wien, Austria and Instituto de Hidrología y Medio Natural, Universidad Politécnica, Valencia, Spain.
authors.forename: K
authors.initials: K
authors.lastname: Geissler
authors.forename: M
authors.initials: M
authors.lastname: Manafi
authors.forename: I
authors.initials: I
authors.lastname: Amorós
authors.forename: J L
authors.initials: JL
authors.lastname: Alonso
mesh_terms: D001431:Bacteriological Techniques; D002863:Chromogenic Compounds / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media; D004755:Enterobacteriaceae / Q000254:growth & development* / Q000302:isolation & purification*; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification*; D005456:Fluorescent Dyes / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media; D005456:Fluorescent Dyes; D001616:beta-Galactosidase; D005966:Glucuronidase