Activation of a capacitative Ca(2+) entry pathway by store depletion in cultured hippocampal neurones.
Intracellular Ca(2+) ([Ca(2+)](i)) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo-3. In the absence of external Ca(2+), the cholinergic agonist carbachol (200 microM) and the sarcoendoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (0.4 microM) both transiently elevated [Ca(2+)](i). A subsequent addition of Ca(2+) into the bathing medium caused a second [Ca(2+)](i) change which was blocked by lanthanum (50 microM). Taken together, these experiments indicate that stores depletion can activate a capacitative Ca(2+) entry pathway in cultured hippocampal neurones and further demonstrate the existence of such a Ca(2+) entry in excitable cells.
Bouron
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A
Department of Pharmacology, University of Bern, Friedbuehlstrasse 49, Bern, Switzerland. alexandre.bouron@pcs.u-bordeaux2.fr
D018750:6-Cyano-7-nitroquinoxaline-2,3-dione / Q000494:pharmacology; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D018679:Cholinergic Agonists / Q000494:pharmacology; D004553:Electric Conductivity; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology* / Q000378:metabolism; D007811:Lanthanum / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013779:Tetrodotoxin / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology; D014966:Xanthenes / Q000378:metabolism
D000814:Aniline Compounds; D018679:Cholinergic Agonists; D018691:Excitatory Amino Acid Antagonists; D014966:Xanthenes; C059715:Fluo-3; D013779:Tetrodotoxin; D019284:Thapsigargin; D007811:Lanthanum; D018750:6-Cyano-7-nitroquinoxaline-2,3-dione; D002217:Carbachol; D000252:Calcium-Transporting ATPases; D011188:Potassium; D002118:Calcium