Quantitative analysis of phagocytosis of Cryptococcus neoformans by adherent phagocytic cells by fluorescence multi-well plate reader.
Macrophages and monocytes are adherent phagocytic cells which play an important role in host defence against the yeast-like fungus Cryptococcus neoformans. Before, phagocytosis by adherent phagocytes could only be measured by means of microscopy or by a radioactive assay, which both have obvious disadvantages. We have developed a new, rapid and objective method to measure phagocytosis of C. neoformans by adherent phagocytes (e.g. alveolar macrophages) using a fluorescence multi-well plate reader. This method allows us to discriminate accurately between adherence and internalisation of C. neoformans by macrophages during long term incubation. In addition, the method was used to study the role of the mannose receptor in phagocytosis of the acapsular yeast in the absence of serum by human monocyte-derived macrophages (MDM).
Walenkamp
A M
AM
The Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Division of Vaccines, University Hospital Utrecht, The Netherlands.
Scharringa
J
J
Schramel
F M
FM
Coenjaerts
F E
FE
Hoepelman
I M
IM
D002448:Cell Adhesion; D003455:Cryptococcus neoformans / Q000276:immunology*; D005453:Fluorescence; D006801:Humans; D037181:Lectins, C-Type; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000382:microbiology; D008351:Mannans / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D009895:Opsonin Proteins; D010587:Phagocytosis / Q000187:drug effects; D011956:Receptors, Cell Surface / Q000378:metabolism
D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D009895:Opsonin Proteins; D011956:Receptors, Cell Surface; D008358:Mannose