Manganese peroxidase isoenzymes produced by Pleurotus ostreatus grown on wood sawdust.
The white rot basidiomycete Pleurotus ostreatus produces two manganese peroxidase (MnP) isoenzymes when grown in solid stationary conditions on poplar sawdust, whereas a lower production of these same enzymes is observed on fir sawdust. Addition of Mn(2+) to poplar culture resulted in a threefold increase of MnP activity; the same addition to fir culture was able to increase tenfold the MnP production. The two MnP isoenzymes (MnP2 and MnP3) were purified from P. ostreatus poplar culture. The isoenzymes differ in their pI values, molecular masses, and N-terminal sequences. MnP3 has the same N-terminal sequence as that of a P. ostreatus MnP previously reported. Both isoenzymes exhibit Mn(2+)-dependent and Mn(2+)-independent peroxidase activities when tested on phenolic substrates. The gene coding for the new isoenzyme MnP2 was cloned and sequenced and the promoter region analyzed. Furthermore, the chromosomal localization of all known P. ostreatus genes was determined.
Giardina
P
P
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, via Mezzocannone, 16, Naples, I-80134, Italy.
Palmieri
G
G
Fontanella
B
B
Rivieccio
V
V
Sannia
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D007526:Isoelectric Point; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008345:Manganese / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014934:Wood
D017931:DNA Primers; D004271:DNA, Fungal; D007527:Isoenzymes; D008345:Manganese; D010544:Peroxidases; C051129:manganese peroxidase