Doublecortin mutations cluster in evolutionarily conserved functional domains.
Mutations in the X-linked gene doublecortin ( DCX ) result in lissencephaly in males or subcortical laminar heterotopia ('double cortex') in females. Various types of mutation were identified and the sequence differences included nonsense, splice site and missense mutations throughout the gene. Recently, we and others have demonstrated that DCX interacts and stabilizes microtubules. Here, we performed a detailed sequence analysis of DCX and DCX-like proteins from various organisms and defined an evolutionarily conserved Doublecortin (DC) domain. The domain typically appears in the N-terminus of proteins and consists of two tandemly repeated 80 amino acid regions. In the large majority of patients, missense mutations in DCX fall within the conserved regions. We hypothesized that these repeats may be important for microtubule binding. We expressed DCX or DCLK (KIAA0369) repeats in vitro and in vivo. Our results suggest that the first repeat binds tubulin but not microtubules and enhances microtubule polymerization. To study the functional consequences of DCX mutations, we overexpressed seven of the reported mutations in COS7 cells and examined their effect on the microtubule cytoskeleton. The results demonstrate that some of the mutations disrupt microtubules. The most severe effect was observed with a tyrosine to histidine mutation at amino acid 125 (Y125H). Produced as a recombinant protein, this mutation disrupts microtubules in vitro at high molar concentration. The positions of the different mutations are discussed according to the evolutionarily defined DC-repeat motif. The results from this study emphasize the importance of DCX-microtubule interaction during normal and abnormal brain development.
Sapir
T
T
Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot, Israel.
Horesh
D
D
Caspi
M
M
Atlas
R
R
Burgess
H A
HA
Wolf
S G
SG
Francis
F
F
Chelly
J
J
Elbaum
M
M
Pietrokovski
S
S
Reiner
O
O
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D017124:Conserved Sequence; D017931:DNA Primers; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D008869:Microtubule-Associated Proteins; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009479:Neuropeptides / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics
C000714328:DCX protein, human; D017931:DNA Primers; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D011994:Recombinant Proteins