Rearrangements of human mitochondrial DNA (mtDNA): new insights into the regulation of mtDNA copy number and gene expression.
Mitochondria from patients with Kearns-Sayre syndrome harboring large-scale rearrangements of human mitochondrial DNA (mtDNA; both partial deletions and a partial duplication) were introduced into human cells lacking endogenous mtDNA. Cytoplasmic hybrids containing 100% wild-type mtDNA, 100% mtDNA with partial duplications, and 100% mtDNA with partial deletions were isolated and characterized. The cell lines with 100% deleted mtDNAs exhibited a complete impairment of respiratory chain function and oxidative phosphorylation. In contrast, there were no detectable respiratory chain or protein synthesis defects in the cell lines with 100% duplicated mtDNAs. Unexpectedly, the mass of mtDNA was identical in all cell lines, despite the fact that different lines contained mtDNAs of vastly different sizes and with different numbers of replication origins, suggesting that mtDNA copy number may be regulated by tightly controlled mitochondrial dNTP pools. In addition, quantitation of mtDNA-encoded RNAs and polypeptides in these lines provided evidence that mtDNA gene copy number affects gene expression, which, in turn, is regulated at both the post-transcriptional and translational levels.
Tang
Y
Y
Department of Genetics and Development, Columbia University, New York, New York 10032, USA. yt62@columbia.edu
Schon
E A
EA
Wilichowski
E
E
Vazquez-Memije
M E
ME
Davidson
E
E
King
M P
MP
D002455:Cell Division; D004272:DNA, Mitochondrial / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D015321:Gene Rearrangement / Q000235:genetics* / Q000502:physiology; D006801:Humans; D006822:Hybrid Cells; D007625:Kearns-Sayre Syndrome / Q000235:genetics* / Q000473:pathology; D010085:Oxidative Phosphorylation; D018741:Replication Origin
D004272:DNA, Mitochondrial