Experimental data proving the presence of inhibitors of amylase activity in biliary and pancreatic juice.
Two parallel studies on albino male rats are performed. In the first study, there is a group which underwent resection of the proximal third of the small intestine. While the other group despite resection of the same segment also has a ligated common biliary and pancreatic duct. In the second study, one group of the experimental animals is only with ligated pancreatic duct and in the other group the same duct is implanted in the initial part of the ileum. On the 15th day after the surgical interventions the amylase activity and the absorption of glucose in the small intestine are studied by the method of turned sacs "in vitro". It is established that the glucose transport does not change after the four surgical interventions. However, the amylase activity increases about twice times after resection of the upper third of the small intestine and more than 4 times after resection of the same segment with simultaneous ligature of the common biliary and pancreas duct. Only at ligating the duct, the amylase activity is decreased in the jejunum and is significantly increased in the ileum, while its implantation in the initial part of the ileum does not change its activity in both studied segments of the small intestine. It is concluded that there are unknown inhibitors for the amylase activity in the biliary and pancreatic juice. The discussed issue is why they inhibit only the enzymatic compensatory processes without influencing the transport systems of the small intestine.
Popov
B
B
Department of Higiene, Medical University, Sofia, Bulgaria.
D000681:Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D001646:Bile / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000502:physiology; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D010189:Pancreatic Juice / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D004791:Enzyme Inhibitors; D000681:Amylases; D005947:Glucose