title: Lysyl-tRNA synthetase of Bacillus stearothermophilus molecular cloning and expression of the gene.
abstract: The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids. The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takita
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Sukata
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: E
authors.initials: E
authors.lastname: Akita
authors.forename: T
authors.initials: T
authors.lastname: Yokota
authors.forename: N
authors.initials: N
authors.lastname: Esaki
authors.forename: K
authors.initials: K
authors.lastname: Soda
authors.forename: K
authors.initials: K
authors.lastname: Inouye
authors.forename: B
authors.initials: B
authors.lastname: Tonomura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000235:genetics*; D008250:Lysine-tRNA Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D003062:Codon; D004269:DNA, Bacterial; D011994:Recombinant Proteins; D008250:Lysine-tRNA Ligase