The genome of Eimeria spp., with special reference to Eimeria tenella--a coccidium from the chicken.
Eimeria spp. contain at least four genomes. The nuclear genome is best studied in the avian species Eimeria tenella and comprises about 60 Mbp DNA contained within ca. 14 chromosomes; other avian and lupine species appear to possess a nuclear genome of similar size. In addition, sequence data and hybridisation studies have provided direct evidence for extrachromosomal mitochondrial and plastid DNA genomes, and double-stranded RNA segments have also been described. The unique phenotype of "precocious" development that characterises some selected lines of Eimeria spp. not only provides the basis for the first generation of live attenuated vaccines, but offers a significant entrée into studies on the regulation of an apicomplexan life-cycle. With a view to identifying loci implicated in the trait of precocious development, a genetic linkage map of the genome of E. tenella is being constructed in this laboratory from analyses of the inheritance of over 400 polymorphic DNA markers in the progeny of a cross between complementary drug-resistant and precocious parents. Other projects that impinge directly or indirectly on the genome and/or genetics of Eimeria spp. are currently in progress in several laboratories, and include the derivation of expressed sequence tag data and the development of ancillary technologies such as transfection techniques. No large-scale genomic DNA sequencing projects have been reported.
Shirley
M W
MW
Institute for Animal Health, Compton Laboratory, Compton, Newbury, UK. shirley@bbsrc.ac.uk
D000818:Animals; D002645:Chickens / Q000469:parasitology*; D002874:Chromosome Mapping; D003062:Codon; D004539:Eimeria / Q000235:genetics*; D016786:Eimeria tenella / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005111:Extrachromosomal Inheritance; D015723:Gene Library; D018503:Genome, Protozoan; D012091:Repetitive Sequences, Nucleic Acid
D003062:Codon