title: Quantitative analysis of acid-base balance in show jumpers before and after exercise.
abstract: The acid-base status of venous blood was studied in 17 show jumpers before and after exercise using both a traditional and a quantitative approach. Partial pressure of carbon dioxide (PCO(2)), pH, haemoglobin, and plasma concentrations of sodium (Na(+)), chloride (Cl(-)), potasium (K(+)), ionized calcium (Ca(2+)), total proteins, albumin, lactate and phosphorus were measured in jugular venous blood samples obtained before and immediately after finishing a show jumping competition. Bicarbonate, anion gap and globulin concentration were calculated from the measured parameters. 'Quantitative analysis' of acid-base balance was performed utilising values for three independent variables: PCO(2), strong ion difference [SID = (Na(+)+ K(+)+ Ca(2+)) - (Cl(-)+ Lact)] and total concentration of weak acids [A(T)= Alb (1 paragraph sign23 pH - 6 paragraph sign31) + Pi (0 paragraph sign309 pH - 0 paragraph sign469) 10/30 paragraph sign97]; plasma concentrations of hydrogen ion ([H(+)]) were also calculated from these variables using Stewart's equation. No significant changes in blood pH were detected after the show jumping competition. Exercise resulted in a significant increase in lactate, Na(+), K(+), haemoglobin, total proteins, albumin, globulin and anion gap, and a decrease in bicarbonate, Cl(-)and Ca(2+). PCO(2)decreased after exercise while SID and A(T)increased. A significant correlation between measured and calculated [H(+)] was found both before and after exercise. However, individual [H(+)] values were not accurately predicted from Stewart's equation. In conclusion, even though pH did not change, significant modifications in the acid-base balance of horses have been found after a show jumping competition. In addition, quantitative analysis has been shown to provide an adequate interpretation of acid-base status in show jumpers before and after exercise.
authors.affiliation: Departamento de Medicina y Cirugía Animal, Universidad de Córdoba, Campus Universitario de Rabanales, Ctra. Madrid-Cádiz km. 396, Córdoba, 14014, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Aguilera-Tejero
authors.forename: J C
authors.initials: JC
authors.lastname: Estepa
authors.forename: I
authors.initials: I
authors.lastname: López
authors.forename: S
authors.initials: S
authors.lastname: Bas
authors.forename: R
authors.initials: R
authors.lastname: Mayer-Valor
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez
mesh_terms: D000136:Acid-Base Equilibrium / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000097:blood; D006736:Horses / Q000502:physiology*; D019344:Lactic Acid / Q000097:blood; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011188:Potassium / Q000097:blood; D011522:Protons; D012964:Sodium / Q000097:blood; D013177:Sports
chemical_list: D011522:Protons; D019344:Lactic Acid; D012964:Sodium; D011188:Potassium; D002118:Calcium