In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats.
To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt-Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased at each time point. Our results suggest that in vivo transfer of the HGF gene into liver accelerates proliferation of hepatic oval cells in the Solt-Farber model in rats.
Shiota
G
G
Second Department of Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Japan. gshiota@grape.med.tottori-u.ac.jp
Kunisada
T
T
Oyama
K
K
Udagawa
A
A
Nomi
T
T
Tanaka
K
K
Tsutsumi
A
A
Isono
M
M
Nakamura
T
T
Hamada
H
H
Sakatani
T
T
Sell
S
S
Sato
K
K
Ito
H
H
Kawasaki
H
H
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000502:physiology*; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D019089:Stem Cell Factor / Q000235:genetics; D013997:Time Factors
D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D019089:Stem Cell Factor; D017228:Hepatocyte Growth Factor; D015073:2-Acetylaminofluorene; D019009:Proto-Oncogene Proteins c-kit; D019859:Proto-Oncogene Proteins c-met