A new technique distinguishing alpha2-3 sialyl linkage from alpha2-6 linkage in sialyllactoses and sialyl-N-acetyllactosamines by post-source decay fragmentation method of MALDI-TOF mass spectrometry.
Alpha2-3 and alpha2-6 sialyl linkage types of sialyllactoses and sialyl-N-acetyllactosamines were analyzed by post-source decay (PSD) fragmentation method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. A new matrix of norharmane was suited for the MALDI-TOF measurements of sialyl oligosaccharides. The fragment ions B1 produced by the cleavage of alpha2-3 sialyl linkages indicate much higher intensity than those produced by the cleavage of alpha2-6 sialyl linkages in sialyllactoses and sialyl-N-acetyllactosamines. Thus, alpha2-3 sialyl linkages cleave much easier than alpha2-6 sialyl linkages in MALDI-PSD fragmentation method. These results suggest that the new techniques using PSD fragmentation of MALDI-TOF mass spectrometry enables us to distinguish alpha2-3 sialyl linkage from alpha2-6 linkage in sialyl oligosaccharides.
Yamagaki
T
T
National Institute of Bioscience & Human-Technology, Tsukuba, Ibaraki, Japan.
Nakanishi
H
H
D000606:Amino Sugars / Q000737:chemistry; D002240:Carbohydrate Sequence; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry; D008969:Molecular Sequence Data; D012794:Sialic Acids / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D000606:Amino Sugars; D012794:Sialic Acids; C032269:sialyl-N-acetyllactosamine; C000020:N-acetylneuraminoyllactose; D007785:Lactose