Characterization of proanthocyanidins from grape seeds.
Leucoselect (grape seed selected proanthocyanidins) was analyzed. HPLC thermospray mass spectrometry (TSP-MS) allowed the detection of monomeric flavan-3-ols and dimeric proanthocyanidins. Fractionation over Sephadex LH-20 resin and analysis of the isolated fractions by gel permeation chromatography (GPC) and electrospray mass spectrometry (ESI-MS) led to the complete characterization of the proanthocyanidin constituents of Leucoselect. The analysis revealed the presence of approximately 15% of (+)-catechin (1) and (-)-epicatechin (2), 80% of (-)-epicatechin 3-O-gallate (3), dimers, trimers, tetramers and their gallates and 5% of pentamers, hexamers, heptamers and their gallates.
Gabetta
B
B
Indena SpA, Laboratori Ricerca e Sviluppo, Via Don Minzoni 6, 20090, Settala, Milano, Italy.
Fuzzati
N
N
Griffini
A
A
Lolla
E
E
Pace
R
R
Ruffilli
T
T
Peterlongo
F
F
D000872:Anthocyanins / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D044945:Proanthocyanidins; D019685:Rosales; D012639:Seeds
D000872:Anthocyanins; D044945:Proanthocyanidins; C013221:proanthocyanidin