[Restriction endonucleases from various bacterial strains exhibit an ice-nucleating activity].
Six strains containing site-specific endonucleases II were selected from a collection of 45 ice-nucleating bacterial strains isolated from rhizosphere of plants growing in various geographical regions. Endonucleases Pfl211I, Psp8I, and Psp23I were isolated and purified from two Pseudomonas sp. strains and a Pseudomonas fluorescens strain. Restriction endonucleases Pfl21I and Psp23I were shown to recognize and cleave the DNA nucleotide sequence 5'-CTGCA decrease G-3'. Endonuclease Psp8I recognized and cleaved the DNA nucleotide sequence 5'-G decrease GATCC-3'. These endonucleases were found to be true isoschizomers of PstI and BamHI, respectively.
Pertsev
A V
AV
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia. pertsev@ibpm.serpukhov.su
Den'mukhametov
M M
MM
Anoshkin
A G
AG
Ariskina
E V
EV
Berezin
I A
IA
Solonin
A S
AS
Kuz'min
N P
NP
D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D007053:Ice; D011549:Pseudomonas / Q000201:enzymology*; D013379:Substrate Specificity
D001426:Bacterial Proteins; D007053:Ice; D015252:Deoxyribonucleases, Type II Site-Specific