Characterization of cephalosporin transfer between aqueous and colloidal phases by micellar electrokinetic chromatography.
A new electrokinetic chromatographic method was applied to the determination of the partition coefficient between water and micelle for a group of cephalosporins (cefmetazol, cephradin, cefaclor, ceftazidim, cefodizim, cephapirin, cephalothin and ceftriaxon) using sodium dodecyl sulphate as an anionic surfactant in microemulsion and in micellar systems. In the new method, the running buffer contains both the micelles and the drug, and the injected solution contains the same concentration of micelles as the running buffer but not the drug. The mobility of the drug can be measured from a negative peak recorded the chromatogram. The required parameters for the determination of the capacity factor (mu(aq) and /mu(me) are the electrophoretic mobilities of the solutes in the aqueous and the micelle phases, mu(eff) is the effective mobility in the micellar system or in the microemulsion) were measured by the new micellar and microemulsion electrokinetic chromatography technique. Linear log-log relationships were found between both the micelle-water partition coefficient and the capacity factor and the n-octanol-water partition coefficient.
Mrestani
Y
Y
Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University, Halle/S., Germany.
Neubert
R
R
D002511:Cephalosporins / Q000737:chemistry* / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
D002511:Cephalosporins; D014867:Water