Angiotensin-converting enzyme polymorphism and the risk of coronary heart disease in the Saudi male population.
OBJECTIVE
To determine the relevance of angiotensin I-converting enzyme (ACE) gene polymorphism for coronary artery disease (CAD) in the Saudi population.


RESULTS
DNA of 84 male Saudi patients with established CAD, 36 male controls who underwent angiography, and 327 healthy Saudi male blood donors was amplified by polymerase chain reaction, using oligonucleotide primers flanking the insertion (I)/deletion (D) sites in the polymorphic region of intron 16 of the ACE gene. Polymerase chain reaction amplification resulted in 490-bp (II), 190-bp (DD), or 490- and 190-bp (ID) fragments. The genotype II distribution was 16.7% in the control group, 7.3% in the blood donor group, and 7.2% in the patients with CAD, and the distribution for DD was 58.3%, 47.1%, and 41.0%, respectively. Notably, 61.9% (P <.0001) of CAD patients presented with angina on admission, and 52.4% had diabetes mellitus.


CONCLUSIONS
The results show no increased risk of CAD in association with either the II or DD genotypes in the Saudi population. However, further investigation of genotype II as a predictor for atherosclerosis rather than increased risk of coronary heart disease may be indicated.
Dzimiri
N
N
Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Basco
C
C
Moorji
A
A
Meyer
B F
BF
D000328:Adult; D001782:Blood Donors; D016022:Case-Control Studies; D003327:Coronary Disease / Q000201:enzymology / Q000453:epidemiology* / Q000235:genetics*; D004251:DNA Transposable Elements; D005838:Genotype; D006801:Humans; D007438:Introns; D008297:Male; D016017:Odds Ratio; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012529:Saudi Arabia / Q000453:epidemiology; D017384:Sequence Deletion
D004251:DNA Transposable Elements; D007703:Peptidyl-Dipeptidase A