The role of ABI3 and FUS3 loci in Arabidopsis thaliana on phase transition from late embryo development to germination.
Arabidopsis abi3 and fus3 mutants are defective in late embryo development and their embryos show precocious growth. To understand the function and role of ABI3 and FUS3, we analyzed expression patterns of genes which were normally activated during late embryo development and germination in these mutants. Using the differential display method, both upregulated and downregulated genes were observed in immature siliques of the abi3 fus3 double mutant. Four clones having more abundant expression in the abi3 fus3 double mutant than in wild type were isolated. These genes were activated during wild-type germination, suggesting that some genes that are activated during wild-type germination are precociously activated in the abi3 fus3 mutant during late embryo development. Also, genes that were activated during wild-type germination were isolated and their expression patterns during late embryo development in the wild type and in abi3, fus3, and abi3 fus3 mutants were analyzed. Sixteen such clones were found, and 11 of these showed derepression or precocious activation of gene expression in the mutants. These results indicate that ABI3 and FUS3 negatively regulate a particular set of genes during late embryo development. We also showed that immature fus3 siliques accumulated one-third of the wild-type level of abscisic acid (ABA), but mature fus3 siliques accumulated ABA at a level comparable to that in the wild type. The possible mechanisms of controlling developmental timing in late embryo development as well as collaborative and distinct roles of ABI3 and FUS3 are discussed.
Nambara
E
E
Division of Applied Bioscience, Hokkaido University, Sapporo, 060-8589, Japan.
Hayama
R
R
Tsuchiya
Y
Y
Nishimura
M
M
Kawaide
H
H
Kamiya
Y
Y
Naito
S
S
D017360:Arabidopsis / Q000196:embryology*; D029681:Arabidopsis Proteins; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D018525:Germination / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D013997:Time Factors; D014157:Transcription Factors; D015854:Up-Regulation
C091601:ABI3 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C075580:FUS3 protein, S cerevisiae; D020928:Mitogen-Activated Protein Kinases