title: SYT-SSX fusion proteins in synovial sarcomas: detection and characterization with new antibodies.
abstract: To identify and characterize the SYT-SSX fusion proteins in synovial sarcomas, we developed two polyclonal antibodies against the N-terminal part and for the C-terminal part of the SYT-SSX2 protein. Specificity was demonstrated on COS-7 cells transfected with two subtypes of SYT-SSX fusion genes, SYT-SSX1 and SYT-SSX2. Both antibodies recognized a single protein of 61 kDa in an immunoprecipitation of the transfected COS-7 cell lysates. These antibodies also detected the native protein of 61 kDa in the lysate of a human synovial sarcoma cell line (HS-SY41) with immunoprecipitation, and in extracts of human synovial sarcomas with western blot analysis. An immunohistochemical study, using human synovial sarcoma tissues, demonstrated that the SYT-SSX fusion proteins localized in the nucleus of the tumor cells. These antibodies provide a useful method for studying the expression of the SYT-SSX fusion proteins.
authors.affiliation: Department of Orthopedic Surgery, Osaka University Medical School, Suita, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hashimoto
authors.forename: N
authors.initials: N
authors.lastname: Araki
authors.forename: H
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: A
authors.initials: A
authors.lastname: Myoui
authors.forename: A
authors.initials: A
authors.lastname: Matsumine
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: H
authors.initials: H
authors.lastname: Sonobe
authors.forename: T
authors.initials: T
authors.lastname: Ochi
mesh_terms: D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D015514:Oncogene Proteins, Fusion / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D013584:Sarcoma, Synovial / Q000276:immunology / Q000378:metabolism*
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D015514:Oncogene Proteins, Fusion; C116512:SYT-SSX fusion protein