[A study on monoclonal antibodies against epithelial cell of endolymphatic sac in guinea pigs].
Using a microdissected endolymphatic sac of the guinea pig (n = 35) as an initial antigen preparation, serials of monoclonal antibodies were established which were used to label the epithelial cells of endolymphatic sac. The antibodies showed strong immunoreactivity with kidney, but not with other organs. It also showed isotopes IgG1, IgG2b and IgM. The results of Western blotting and SDS-PAGE indicated that the epitopes of monoclonal antibodies were proteins or glyoprotein with a molecular weight of approximately 7,400. The locations of the epitopes in epithelial cells suggest that it may play some roles in construction and function of the endolymphatic sac.
Zhang
R
R
First Affiliated Hospital, Fujian Medical College, Fuzhou.
Hozawa
K
K
Wataya
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004712:Endolymphatic Sac / Q000166:cytology / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology*; D006168:Guinea Pigs; D006825:Hybridomas / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M