Assays for activation of recombinant expressed opsins by all-trans-retinals.
Han
M
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
Sakmar
T P
TP
D019943:Amino Acid Substitution; D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012172:Retinaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D017299:Rod Opsins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014162:Transfection
D011994:Recombinant Proteins; D017299:Rod Opsins; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D012243:Rhodopsin; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D012172:Retinaldehyde