title: The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd) is localized centromeric to the CD1 gene cluster on human Chromosome 1.
abstract: The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd), was isolated from a human Chromosome (Chr) 1 cosmid library (LLNL, UK HGMP). The gene spans 11 kilobases and contains nine exons and eight introns. Four alternative transcript variants were observed in activated T cells. Three single-nucleotide polymorphisms were identified within intron 2. A variable number of GA repeats was found at position -340 from the first coding ATG. Linkage analysis using this marker in eight CEPH families showed that the SH2D2A gene is located close to the D1S2624 marker on Chr 1q21-1q22. Physical mapping of a PAC and BAC contig containing the CD1 gene cluster telomeric to D1S2624 failed to identify a clone harboring the SH2D2A gene. Thus the SH2D2A gene is located centromeric to the CD1 gene cluster on Chr 1.
authors.affiliation: Institute of Immunology, The National Hospital, Oslo, Norway.
authors.forename: K Z
authors.initials: KZ
authors.lastname: Dai
authors.forename: G
authors.initials: G
authors.lastname: Vergnaud
authors.forename: A
authors.initials: A
authors.lastname: Ando
authors.forename: H
authors.initials: H
authors.lastname: Inoko
authors.forename: A
authors.initials: A
authors.lastname: Spurkland
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D017398:Alternative Splicing; D018949:Antigens, CD1 / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002503:Centromere; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes; D018909:src Homology Domains
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018949:Antigens, CD1; D002352:Carrier Proteins; D018076:DNA, Complementary; C405691:SH2D2A protein, human