title: An in vitro system from Plasmodium falciparum active in endogenous mRNA translation.
abstract: An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.
authors.affiliation: Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad de Carabobo, Maracay, Aragua, Venezuela.
authors.forename: A
authors.initials: A
authors.lastname: Ferreras
authors.forename: L
authors.initials: L
authors.lastname: Triana
authors.forename: H
authors.initials: H
authors.lastname: Correia
authors.forename: E
authors.initials: E
authors.lastname: Sánchez
authors.forename: F
authors.initials: F
authors.lastname: Herrera
mesh_terms: D000818:Animals; D002474:Cell-Free System; D004912:Erythrocytes / Q000469:parasitology; D010963:Plasmodium falciparum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D016053:RNA, Protozoan / Q000032:analysis*
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan