Estrogen receptor beta mRNA in colon cancer cells: growth effects of estrogen and genistein.
Knowledge regarding the expression of the recently cloned estrogen receptor beta (ERbeta) in colonic mucosa is limited. In this study, we demonstrated that five human colon cancer cell lines, HT29, Colo320, Lovo, SW480, and HCT116, expressed ERbeta mRNA, but lacked ERalpha mRNA. Results from a cell growth assay demonstrated that these colon cancer cells were not influenced by estrogen, while genistein possessed slight growth inhibitory effects on HT29, Colo320 and Lovo cells at 10 microM, at which concentration is stimulated the growth of ERalpha-positive human breast cancer MCF-7 cells. Tamoxifen inhibited the growth of HT29 and Colo320 cells, dose-dependently, as well as MCF-7 cells. A transfected reporter plasmid containing a vitellogenin estrogen response element could be activated by estradiol in Colo320 cells. Taken together with previous reports, these data suggest that ERalpha and ERbeta may have different biological functions in colon cells.
Arai
N
N
Department of Medical Nutrition, Karolinska Institute, NOVUM, Huddinge, S-141 84, Sweden.
Ström
A
A
Rafter
J J
JJ
Gustafsson
J A
JA
D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics; D004815:Epidermal Growth Factor / Q000494:pharmacology; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*; D013629:Tamoxifen / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics; D000070584:Tripartite Motif Proteins; D014407:Tumor Cells, Cultured; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers
D017931:DNA Primers; D004268:DNA-Binding Proteins; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004967:Estrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D013629:Tamoxifen; D004815:Epidermal Growth Factor; D019833:Genistein; C084523:TRIM25 protein, human; D044767:Ubiquitin-Protein Ligases