The cancer germ-line genes MAGE-1, MAGE-3 and PRAME are commonly expressed by human myeloma cells.
In this study, we have investigated the mRNA expression of the cancer germ-line genes MAGE, BAGE, GAGE, RAGE and the tumor-overexpressed gene PRAME by human myeloma cell lines and malignant plasma cells from patients with multiple myeloma (MM). By reverse transcription-PCR, we show that all myeloma cell lines (n = 16) express at least one of these genes, except RAGE-1 that was never expressed. We show that malignant plasma cells from the majority of MM patients (n = 21) expressed MAGE-1, MAGE-3 and PRAME. On the contrary, polyclonal reactive plasma cells did not express any of these genes. By flow cytometry, we show that mage-1 protein is expressed within myeloma cells and cell lines and that anti-mage-1.HLA-A1 cytotoxic T lymphocytes efficiently killed MAGE-1+HLA-A1+ MDN myeloma cells. Taken together, our data show that mage-1 and mage-3 could constitute specific targets for tumor immunotherapy of MM patients.
Pellat-Deceunynck
C
C
Inserm U463, Institut de Biologie, Nantes, France. cpellat@nantes.inserm.fr
Mellerin
M P
MP
Labarrière
N
N
Jego
G
G
Moreau-Aubry
A
A
Harousseau
J L
JL
Jotereau
F
F
Bataille
R
R
D000951:Antigens, Neoplasm; D001402:B-Lymphocytes / Q000276:immunology; D005136:Eye Proteins / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D058965:MART-1 Antigen; D058950:Melanoma-Specific Antigens; D009101:Multiple Myeloma / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics*; D009857:Oncogenes; D010950:Plasma Cells / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; C092706:BAGE protein, human; D005136:Eye Proteins; C095365:GAGE1 protein, human; C095366:GAGE2A protein, human; C072011:MAGEA1 protein, human; C072013:MAGEA3 protein, human; D058965:MART-1 Antigen; C548667:MLANA protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; C404944:PRAME protein, human; D012333:RNA, Messenger; D012334:RNA, Neoplasm