title: Microdissection and FISH investigations in acute myeloid leukemia: a step forward to full identification of complex karyotypic changes.
abstract: Complex chromosomal rearrangements in malignant hemopathies frequently remain unclarified because of paucity of material for further fluorescence in situ hybridization analyses and/or lack of suitable probes. Chromosome microdissection (MD) can be an adequate approach to elucidate chromosome aberrations unrecognizable by conventional karyotyping. We applied MD in two patients with acute myeloid leukemia (AML) and unidentified chromosome changes at karyotype. Microdissection of a ring chromosome in an AML-M5 case revealed 21q polysomy. In an AML-M4 case, MD of an add(15p) disclosed a t(8;15) with over-representation of both 8q22 and 8q24 bands. YAC probes were helpful in showing duplication of the ETO gene at 8q22, and amplification of C-MYC, at 8q24.
authors.affiliation: Department of Hematology, University of Perugia, Perugia, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Falzetti
authors.forename: J R
authors.initials: JR
authors.lastname: Vermeesch
authors.forename: C
authors.initials: C
authors.lastname: Matteucci
authors.forename: S
authors.initials: S
authors.lastname: Ciolli
authors.forename: M F
authors.initials: MF
authors.lastname: Martelli
authors.forename: P
authors.initials: P
authors.lastname: Marynen
authors.forename: C
authors.initials: C
authors.lastname: Mecucci
mesh_terms: D000782:Aneuploidy; D002869:Chromosome Aberrations / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D005260:Female; D005784:Gene Amplification / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D007948:Leukemia, Monocytic, Acute / Q000235:genetics*; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012303:Ring Chromosomes; D014178:Translocation, Genetic
chemical_list: D015342:DNA Probes