title: The effect of vigorous fluid resuscitation in uncontrolled hemorrhagic shock after massive splenic injury.
abstract: OBJECTIVE
  Using a standardized massive splenic injury model of uncontrolled hemorrhagic shock, we studied the effect of vigorous fluid resuscitation on the hemodynamic response and survival time in rats.
  
  
  METHODS
  Randomized, controlled study. Duration of follow-up was 4 hrs.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Adult male Sprague-Dawley rats, weighing 240-430 g.
  
  
  METHODS
  Standardized massive splenic injury was induced by two transverse incisions in the rat's spleen. The animals were randomized into four groups: group 1 (n = 8) underwent sham operation; in group 2 (n = 15), massive splenic injury was untreated; in group 3 (n = 15), massive splenic injury was treated with 41.5 mL/kg 0.9% sodium chloride (large-volume normal saline); and in group 4 (n = 15), massive splenic injury was treated with 5 mL/kg 7.5% sodium chloride (hypertonic saline).
  
  
  RESULTS
  The hemodynamic and metabolic variables in the sham-operated group 1 were stable throughout the experiment. Mean arterial pressure in group 2 decreased from 86.5 +/- 4.0 to 50.3 +/- 6.3 mm Hg (p < .001) in the first 15 mins after massive splenic injury. Mean survival time in group 2 was 127.5 +/- 17.0 mins; total blood loss was 33.8% +/-2.6% of blood volume; and the mortality rate at 1 hr was 13.3%. Bolus infusion of large-volume normal saline after 15 mins resulted in an early increase in mean arterial pressure from 48.6 +/-7.4 to 83.3 +/- 7.2 mm Hg (p < .01); it then rapidly decreased to 24.6 +/- 8.6 mm Hg (p < .001) after 60 mins. The mean survival time (95.3 +/- 16.4 mins) was significantly lower than in group 2 (p < .01); total blood loss (48.0% +/- 4.3%) was significantly higher than in group 2 (p < .01); and mortality rate in the first hour was 33.3% (p < .05). Bolus infusion of hypertonic saline also decreased survival time to 93.3 +/- 20.3 mins (p < .01), but total blood loss was 35.2% +/- 3.0%, which was not significantly different from the blood loss in group 2. The mortality rate in the first hour (60.0%) was significantly higher than in group 2 (p < .005).
  
  
  CONCLUSIONS
  Vigorous infusion of normal saline after massive splenic injury resulted in a significant increase in intra-abdominal bleeding and decreased survival time. The hemodynamic response to crystalloid infusion in blunt abdominal trauma is primarily dependent on the severity of injury and the rate of blood loss.
authors.affiliation: Department of Surgery A, Rambam Medical Center, Haifa, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Solomonov
authors.forename: M
authors.initials: M
authors.lastname: Hirsh
authors.forename: A
authors.initials: A
authors.lastname: Yahiya
authors.forename: M M
authors.initials: MM
authors.lastname: Krausz
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005440:Fluid Therapy; D008297:Male; D015999:Multivariate Analysis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation / Q000379:methods*; D012771:Shock, Hemorrhagic / Q000209:etiology / Q000628:therapy*; D013154:Spleen / Q000293:injuries*; D016019:Survival Analysis; D014949:Wounds, Nonpenetrating / Q000150:complications / Q000628:therapy