title: The determination of delta9-tetrahydrocannabinol and 11-nor-9-carboxy-delta9-tetrahydrocannabinol in whole blood using solvent extraction combined with polar solid-phase extraction.
abstract: A method for the determination of delta9-tetrahydrocannabinol (THC) and 11-nor-9-carboxy-delta9-tetrahydrocannabinol (THCCOOH) in whole blood using conventional solvent extraction followed by a cleanup using polar solid-phase cartridges is described. The method uses 0.5 mL of blood, and detection is by benchtop gas chromatography-mass selective detection using selected ion monitoring. The limit of detection is better than 1 ng/mL, and extraction efficiencies are greater than 80% for THC and 70% for THCCOOH. The method is robust and highly reproducible.
authors.affiliation: Department for Administrative and Information Services, Forensic Science Centre, Adelaide, South Australia, Australia.
authors.forename: P D
authors.initials: PD
authors.lastname: Felgate
authors.forename: A C
authors.initials: AC
authors.lastname: Dinan
mesh_terms: D013759:Dronabinol / Q000031:analogs & derivatives* / Q000097:blood* / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D015813:Substance Abuse Detection / Q000379:methods
chemical_list: D012997:Solvents; C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; D013759:Dronabinol