title: Cutaneous CD30+ cells in children with atopic dermatitis.
abstract: BACKGROUND
  CD30 expression can be considered a marker of Th2 cells. We investigated the presence of CD30+ cells in the lesional skin of children with atopic dermatitis (AD). We also analyzed the possible relationship between CD30+ cells and serum soluble CD 30 (sCD30) levels, and IgE, soluble interleukin-2 (IL-2) receptor (sIL-2R) or soluble CD23 (sCD23) levels in the blood, and clinical score.
  
  
  METHODS
  Ten eczematous children (4 males, 6 females; median age: 4 years and 5 months; range: 11 months to 14 years), 9 sex- and age-matched control children and an adult control group were studied. A clinical score (SCORAD index), was given to eczematous lesions. Blood was taken for the determination of IgE, sCD30, sIL-2R and sCD23 levels. Punch biopsies of lesional skin were stained with hematoxylin and eosin or incubated with anti-CD30 monoclonal antibodies. Skin prick tests (SPTs) were also performed.
  
  
  RESULTS
  In the biopsy specimens, CD30 expression was observed in high proportions of infiltrating cells. In children with AD, total serum IgE, sCD30, sIL-2R, sCD23 and eosinophils were significantly elevated compared to controls. CD30+ cells were not associated with serum IgE, sCD30, sIL-2R, sCD23, or SPT results, score of inflammatory cells in lesional skin or clinical score. Children with AD who had high total IgE and specific IgE antibodies did not differ from those with normal total IgE and negative specific IgE in respect of age, clinical score, number of CD30+ cells, sCD30, sIL-2R and sCD23 levels, score of inflammatory cells in skin or clinical score.
  
  
  CONCLUSIONS
  Our results showed remarkable numbers of CD30+ cells in the lesional skin and high sCD30 in the serum of children with AD. CD30+ cells did not correlate with systemic markers of IgE reaction.
authors.affiliation: Dipartimento Pediatria, Azienda Sanitaria, Modena, Italy. g.cavagni@ausl.mo.it
authors.forename: G
authors.initials: G
authors.lastname: Cavagni
authors.forename: C
authors.initials: C
authors.lastname: Caffarelli
authors.forename: F
authors.initials: F
authors.lastname: Facchetti
authors.forename: D
authors.initials: D
authors.lastname: Brugnoni
authors.forename: L D
authors.initials: LD
authors.lastname: Notarangelo
authors.forename: C
authors.initials: C
authors.lastname: Tosoni
authors.forename: R
authors.initials: R
authors.lastname: Altobelli
mesh_terms: D000293:Adolescent; D000328:Adult; D002465:Cell Movement / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology* / Q000473:pathology; D004485:Eczema / Q000097:blood / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D017730:Ki-1 Antigen / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D017455:Receptors, IgE / Q000097:blood; D015375:Receptors, Interleukin-2 / Q000097:blood; D012867:Skin / Q000737:chemistry / Q000276:immunology* / Q000473:pathology
chemical_list: D017730:Ki-1 Antigen; D017455:Receptors, IgE; D015375:Receptors, Interleukin-2; D007073:Immunoglobulin E