Sexing of chicken feather follicle, blastodermal and blood cells.
The production of chicken chimeras using donor and acceptor cells which can be of opposite sex has necessitated the utilization of methods developed to distinguish the sex of chickens. We demonstrate one of these methods, based on the polymerase chain reaction which amplifies the EcoRI repeat unit of the fowl W chromosome, and how this technique may be used to sex various cell types in chickens as well as small numbers of blastodermal cells. Our results demonstrate the ability to sex chickens using EcoRI primers, specific for the W chromosome, from as little as 2 ng of female genomic DNA isolated from blood and feathers--the latter being the result of DNA extraction from only one feather. Also evident in this study is the detection of the W chromosome by PCR from approximately 50 blastodermal cells originating from the developing blastodisc at stage X.
Trefil
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P
Biopharm, Research Institute of Biopharmacy and Veterinary Drugs, Inc., Jílové u Prahy, Czech Republic.
Bruno
M M
MM
Mikus
T
T
Thoraval
P
P
D000818:Animals; D001756:Blastoderm / Q000166:cytology*; D001773:Blood Cells / Q000737:chemistry*; D002642:Chick Embryo; D002645:Chickens / Q000502:physiology*; D002678:Chimera; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D005241:Feathers / Q000737:chemistry*; D005260:Female; D008297:Male; D010858:Pigmentation / Q000235:genetics; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012730:Sex Chromosomes / Q000737:chemistry; D012732:Sex Determination Analysis / Q000379:methods*
D004247:DNA; D015246:Deoxyribonuclease EcoRI