[Mutation analysis of transforming growth factor-beta type II receptor gene in human gastric cancer tissues].
OBJECTIVE
Mutations which cause abarrent expression of transforming type II receptor (TGF-beta RII) are responsible for the escape of some tumor cells from growth control midiated by TGF-beta. Two mutation hot spots (cDNA 709-718, 1931-1936) have been reported on human colon cancer cell lines. To elucidate the situation of this gene in human gastric cancer, 44 gastric cancer tissues were examined for mutation at the two hot spots.


METHODS
Genomic DNA extracted from the cancer tissues was PCR amplified and analysed by silver stain for single stranded conformation polymorphism. Positive samples were sequenced by dry-primer automatic sequencing method.


RESULTS
The data revealed a deletion rate of 6.8% (3/44) of the 1st hot spot; CA insertion of the 2nd hot spot was not found in all 44 gastric cancer tissues.


CONCLUSIONS
The mutation incidence of TGF-beta R II in human gastric cancer is low.
Ke
Y
Y
Beijing Institute for Cancer Research.
Hagiwara
K
K
Su
X
X
D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*
D018125:Receptors, Transforming Growth Factor beta