Nonenzymatic template-directed reactions on altritol oligomers, preorganized analogues of oligonucleotides.
Altritol nucleic acids (ANAs) are RNA analogues with a phosphorylated D-altritol backbone. The nucleobase is attached at the 2-(S)-position of the carbohydrate moiety. We report that ANA oligomers are superior to the corresponding DNA, RNA, and HNA (hexitol nucleic acid) in supporting efficient nonenzymatic template-directed synthesis of complementary RNAs from nucleoside-5'-phosphoro-2-methyl imidazolides. Activated ANA and HNA monomers do not oligomerize efficiently on DNA, RNA, HNA, or ANA templates.
Kozlov
I A
IA
The Salk Institute for Biological Studies, San Diego, California 92186, USA.
Zielinski
M
M
Allart
B
B
Kerremans
L
L
Van Aerschot
A
A
Busson
R
R
Herdewijn
P
P
Orgel
L E
LE
D002621:Chemistry / Q000379:methods; D004247:DNA / Q000737:chemistry*; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives / Q000737:chemistry; D009696:Nucleic Acids / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013402:Sugar Alcohols / Q000737:chemistry*; D013698:Templates, Genetic
D009696:Nucleic Acids; D009841:Oligonucleotides; D013402:Sugar Alcohols; C042890:altritol; D012313:RNA; C036896:guanosine 5'-phospho-2-methylimidazolide; D006157:Guanosine Monophosphate; D004247:DNA