title: Characterization of newly developed SSLP markers for the rat.
abstract: We have isolated more than 12,000 clones containing microsatellite sequences, mainly consisting of (CA)n dinucleotide repeats, using genomic DNA from the BN strain of laboratory rat. Data trimming yielded 9636 non-redundant microsatellite sequences, and we designed oligonucleotide primer pairs to amplify 8189 of these. PCR amplification of genomic DNA from five different rat strains yielded clean amplification products for 7040 of these simple-sequence-length-polymorphism (SSLP) markers; 3019 markers had been mapped previously by radiation hybrid (RH) mapping methods (Nat Genet 22, 27-36, 1998). Here we report the characterization of these newly developed microsatellite markers as well as the release of previously unpublished microsatellite marker information. In addition, we have constructed a genome-wide linkage map of 515 markers, 204 of which are derived from our new collection, by genotyping 48 F2 progeny of (OLETFxBN)F2 crosses. This map spans 1830.9 cM, with an average spacing of 3.56 cM. Together with our ongoing project of preparing a whole-genome radiation hybrid map for the rat, this dense linkage map should provide a valuable resource for genetic studies in this model species.
authors.affiliation: Otsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
authors.forename: T K
authors.initials: TK
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Ono
authors.forename: S
authors.initials: S
authors.lastname: Okuno
authors.forename: A
authors.initials: A
authors.lastname: Mizoguchi-Miyakita
authors.forename: Y
authors.initials: Y
authors.lastname: Yamasaki
authors.forename: N
authors.initials: N
authors.lastname: Kanemoto
authors.forename: H
authors.initials: H
authors.lastname: Hishigaki
authors.forename: K
authors.initials: K
authors.lastname: Oga
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Irie
authors.forename: M T
authors.initials: MT
authors.lastname: Bihoreau
authors.forename: M R
authors.initials: MR
authors.lastname: James
authors.forename: G M
authors.initials: GM
authors.lastname: Lathrop
authors.forename: T
authors.initials: T
authors.lastname: Takagi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Tanigami
mesh_terms: D000818:Animals; D008040:Genetic Linkage; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D013045:Species Specificity
chemical_list: D005819:Genetic Markers