[Information behavior of microsatellite loci in genome scanning].
OBJECTIVE
To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population.


METHODS
Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan( TM) and Genotyper(TM).


RESULTS
The heterozygosities are between 0.35 to 0.89, with the heterozygosities of 88% loci >0.60. PICs are in the range of 0.32 and 0.88, with PICs of 95% loci >0.50.


CONCLUSIONS
The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.
Yuan
W
W
Molecular Medical Center, Ruijin Hospital, Shanghai Second Medical University, Shanghai, 200025 P. R. China.
Xu
H
H
Zhao
J
J
Ding
W
W
Jiang
H
H
Gu
M
M
Xue
J
J
Chen
J
J
Fang
F
F
Chen
Z
Z
Jin
L
L
Huang
W
W
D000368:Aged; D000369:Aged, 80 and over; D002874:Chromosome Mapping; D005260:Female; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic