title: DR2 antigens are associated with severity of disease in toxic oil syndrome (TOS).
abstract: Toxic oil syndrome (TOS) was an epidemic which broke out in Spain in 1981, caused by the ingestion of rapeseed oil denatured with 2% aniline and sold illegally as edible oil. More than 20,000 people were affected and mortality rate was 8.4%. Genetic susceptibility appears to be involved in the pathology of this disease. Several reports have described association between the chronic stage of the disease and DR-DQ antigens (DR3, DR4, DR2 and DQ8). In the present work, we have reassessed the HLA class II antigens in a well-designed case-control study. Triplets of subjects (n=265) composed by chronic patients (n=117), non-affected family members (n=71) and non-related controls (n=77) were studied. Also, HLA class II antigens were analyzed in patients who had died from TOS (n= 34) and in TOS control patients who died from other non-TOS related causes (n=13). Regarding surviving patients no significant association was found between HLA and disease. In contrast, an increase in phenotypic frequency of DR2 antigen, was found in patients who had died from TOS (73.5%) compared with the whole study group: TOS-affected alive patients (25.6%, corrected P<0.001), non-affected family members (28.5%, corrected P<0.001), non-related controls (23.9%, corrected P<0.001) and dead controls (38.4%, P=0.03).
authors.affiliation: Immunology Department, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Cárdaba
authors.forename: J
authors.initials: J
authors.lastname: Ezendam
authors.forename: S
authors.initials: S
authors.lastname: Gallardo
authors.forename: V
authors.initials: V
authors.lastname: del Pozo
authors.forename: M
authors.initials: M
authors.lastname: Izquierdo
authors.forename: C
authors.initials: C
authors.lastname: Martín
authors.forename: I
authors.initials: I
authors.lastname: Cortegano
authors.forename: E
authors.initials: E
authors.lastname: Aceituno
authors.forename: M
authors.initials: M
authors.lastname: Rojo
authors.forename: I
authors.initials: I
authors.lastname: Arrieta
authors.forename: P
authors.initials: P
authors.lastname: Palomino
authors.forename: M
authors.initials: M
authors.lastname: Posada
authors.forename: C
authors.initials: C
authors.lastname: Lahoz
mesh_terms: D000814:Aniline Compounds / Q000009:adverse effects*; D001327:Autoimmune Diseases / Q000453:epidemiology / Q000276:immunology* / Q000503:physiopathology; D016022:Case-Control Studies; D002908:Chronic Disease; D004196:Disease Outbreaks; D005229:Fatty Acids, Monounsaturated; D015802:HLA-DR2 Antigen / Q000032:analysis* / Q000145:classification / Q000235:genetics; D016612:Paraffin Embedding; D010938:Plant Oils / Q000009:adverse effects*; D000074262:Rapeseed Oil; D013030:Spain / Q000453:epidemiology; D017741:Survivors; D013577:Syndrome
chemical_list: D000814:Aniline Compounds; D005229:Fatty Acids, Monounsaturated; D015802:HLA-DR2 Antigen; D010938:Plant Oils; D000074262:Rapeseed Oil; C023650:aniline