Herpes simplex virus UL17 protein is associated with B capsids and colocalizes with ICP35 and VP5 in infected cells.
A previous study using a mutant lacking the UL17 gene has suggested that the UL17 protein of herpes simplex virus type 1 (HSV-1) is required for the cleavage/packaging of viral DNA. In this study, we have raised a rabbit polyclonal antiserum which specifically reacted with the UL17 protein which has an apparent molecular mass of 78-kDa in the lysates of HSV types 1- and 2-infected Vero cells. Western blot analysis of intracellular capsids demonstrates that the UL17 protein was associated with B and C capsids. Indirect immunofluorescence studies reveal that it colocalized with the major capsid protein VP5 and the scaffolding protein ICP35 within the nucleus. These results suggest that the association of the UL17 protein with immature B-type capsids is important for its role in cleavage/packaging.
Goshima
F
F
Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Watanabe
D
D
Takakuwa
H
H
Wada
K
K
Daikoku
T
T
Yamada
M
M
Nishiyama
Y
Y
D000818:Animals; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins; D002522:Chlorocebus aethiops; D019084:Fluorescent Antibody Technique, Indirect; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000502:physiology; D006801:Humans; D018613:Microscopy, Confocal; D011817:Rabbits; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D036022:Capsid Proteins; C054582:ICP35 protein, Human herpesvirus 1; C442523:VP5 protein, Herpes simplex virus type 1; D014764:Viral Proteins; C069185:DNA cleavage and packaging proteins, Herpesvirus