[Study on the synthesis and secretion rate of transferrin in hepatoma and peritumor hepatocytes].
OBJECTIVE
To study the changes of synthesis and secretion of transferrin (Tf) in human hepatocellular carcinoma (HCC) cells and search the causes of reduced membrane-bond transferrin in HCC tissue.


METHODS
By radioisotope incorporation and immunoprecipitation to determine the synthesis and secretion rate of transferrin in human HCC cells and peritumor hepatocytes.


RESULTS
When compared with peritumor hepatocytes the quantity of transferrin synthesized was not changed in HCC cells, but its secretion rate was accelerated significantly while the transferrin storage was significantly decreased.


CONCLUSIONS
The results suggest that HCC growth might be enhanced by increased Tf secretion and help explain the decreased membrane-bond form of Tf in HCC tissue.
Xie
T
T
Tumor Immunology and Gene Therapy Center, Second Military Medical University, Shanghai.
Wu
M
M
Shen
F
F
D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000503:physiopathology*; D011233:Precipitin Tests; D014168:Transferrin / Q000096:biosynthesis / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D014168:Transferrin