Haemostatic factors in human peripheral afferent lymph.
Peripheral afferent lymph was obtained by cannulation of a collecting vessel in 17 healthy men (mean age 26 years). Lymph/plasma ratios of all vitamin K-dependent factors were lower than expected from molecular weight. Factor VII, factor IX and tissue factor pathway inhibitor (TFPI) lymph/plasma activity ratios were higher than antigen ratios. Activated factor VII (FVIIa) and TFPI-Xa complex concentrations were higher in lymph than plasma, and the raised FVIIa did not appear to be due to cannulation. The fibrinogen lymph/plasma activity (Clauss) ratio averaged about 20% of the antigen ratio. The result of an ELISA for D-dimer was higher in lymph than plasma, often more than five-fold. This high level in lymph was not explored but may indicate proteolysis of fibrinogen and fibrin with release of D-like and D-dimer-like fragments in interstitial fluid.
Miller
G J
GJ
Medical Research Council Epidemiology and Medical Care Unit, London, UK. g.miller@mds.qmw.ac.uk
Howarth
D J
DJ
Attfield
J C
JC
Cooke
C J
CJ
Nanjee
M N
MN
Olszewski
W L
WL
Morrissey
J H
JH
Miller
N E
NE
D000328:Adult; D005164:Factor IX / Q000378:metabolism; D005167:Factor VII / Q000378:metabolism; D015942:Factor VIIa / Q000378:metabolism; D015951:Factor Xa / Q000378:metabolism; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000097:blood; D006487:Hemostasis / Q000502:physiology*; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000378:metabolism; D008196:Lymph / Q000502:physiology*; D008297:Male
D005338:Fibrin Fibrinogen Degradation Products; D005343:Fibrinolytic Agents; D008074:Lipoproteins; C036309:fibrin fragment D; C051928:lipoprotein-associated coagulation inhibitor; D005167:Factor VII; D005164:Factor IX; D005340:Fibrinogen; D015942:Factor VIIa; D015951:Factor Xa