The salivary 5'-nucleotidase/phosphodiesterase of the hematophagus sand fly, Lutzomyia longipalpis [corrected].
Salivary gland homogenates from adult female Lutzomyia longipalpis sand flies contain large amounts of 5'-nucleotidase and phosphodiesterase activities. Phosphodiesterase activity was found to be associated with 5'-nucleotidase in several independent experiments: (i) it coelutes with 5'-nucleotidase on a molecular sieving column, (ii) it coelutes with 5'-nucleotidase on a chromatofocusing column, and (iii) it has the same thermal inactivation kinetics as the 5'-nucleotidase activity. Additionally, both activities are independent of divalent cations, and both are decreased following a blood meal, suggesting that they reside in the same molecule. The role of salivary nucleotidases and purine nucleotides in blood-feeding by sand flies is discussed.
Ribeiro
J M
JM
Section of Medical Entomology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892-0425, USA. jribeiro@nih.gov
Rowton
E D
ED
Charlab
R
R
D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D005260:Female; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011576:Psychodidae / Q000201:enzymology*; D012469:Salivary Glands / Q000201:enzymology*
D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases