A novel HLA-B*51 allele (B*5116) identified by nucleotide sequencing.
We report here an additional HLA-B*51 variant designated HLA-B*5116. Detected by an abnormal serological reactivity pattern, this variant was identified as a B*51 allele by polymerase chain reaction using sequence-specific primers (PCR-SSP) and characterized by nucleotide sequencing. The new variant sequence match closely with the classical HLA-B*5101 excepted two adjacent nucleotide substitutions at positions 216 and 217 of the third exon and the subsequent Leucine to Glutamic acid change at codon 163 of the alpha2 domain (CTG-->GAG). This new variant was not detected in three different ethnic groups (French, Algerian and Lebanese) suggesting a very rare frequency.
Tamouza
R
R
Laboratoire d'Immunologie et d'Histocompatibilité, Hôpital Saint Louis, and INSERM U396, Paris, France. tamouza@histo.chu-stlouis.fr
Carbonnelle
E
E
Schaeffer
V
V
Sadki
K
K
Abed
Y
Y
Marzais
F
F
Poirier
J C
JC
Fortier
C
C
Toubert
A
A
Raffoux
C
C
Charron
D
D
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D059949:HLA-B51 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D015235:HLA-B Antigens; D059949:HLA-B51 Antigen