Low surfactant concentration increases fungal mineralization of a polychlorinated biphenyl congener but has no effect on overall metabolism.
Three white rot fungi were compared for their ability to attack polychlorinated biphenyl (PCB) congeners in the presence and absence of the non-ionic Triton X-100 or the anionic Dowfax 8390 surfactants at half their critical micelle concentrations. Neither surfactant affected PCB biodegradation monitored by gas chromatography but the release of 14CO2 from 2,4',5-[U-14C]trichlorobiphenyl by Trametes versicolor was stimulated 12% by Triton X-100. Since mineralization is the complete metabolism of the congener and biodegradation was measured as substrate disappearance, Triton X-100 is proposed to aid intracellular solubilization of 2,4',5-trichlorobiphenyl for complete oxidation by T. versicolor.
Beaudette
L A
LA
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Ward
O P
OP
Pickard
M A
MA
Fedorak
P M
PM
D001487:Basidiomycota / Q000187:drug effects* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media; D017830:Octoxynol / Q000494:pharmacology; D010544:Peroxidases / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003470:Culture Media; D013501:Surface-Active Agents; C015550:2,4',5-trichlorobiphenyl; D017830:Octoxynol; D011078:Polychlorinated Biphenyls; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; C030375:2,3-dichlorobiphenyl