Detection of intact megaDalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry.
Well-resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2%. The mass spectral data seem to reflect known solution-phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and pH.
van Berkel
W J
WJ
Department of Biomolecular Sciences, Wageningen University, The Netherlands.
van den Heuvel
R H
RH
Versluis
C
C
Heck
A J
AJ
D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008958:Models, Molecular; D008970:Molecular Weight; D009994:Osmolar Concentration; D020836:Protein Structure, Quaternary
D000429:Alcohol Oxidoreductases; C076865:vanillyl-alcohol oxidase