title: Experimental design and multivariate calibration in the development, set-up and validation of a differential pulse polarographic and UV spectrophotometric method for the simultaneous plasmatic determination of the therapeutic metronidazole-pefloxacin combination.
abstract: Partial least squares regression (PLS1 and PLS2) and GOLPE variable selection procedures were used for the treatment of differential pulse polarographic and UV spectrophotometric data obtained from the analysis of the therapeutic combination of metronidazole and pefloxacin. The analytical method used for the determination was set up using experimental design strategies (Doehlert's design, full factorial design, fractional face center cube design, etc.) and by involving the simultaneous optimization of several responses (desirability function). Method validation was also performed, determining accuracy, precision, linearity and range, detection and quantification limits and robustness. The quantitative prediction abilities in determining metronidazole and pefloxacin plasma levels of the PLS1 and PLS2 models were tested on spiked plasma samples and good results were obtained (metronidazole, 97.5%, RSD = 4.8%, n = 3; pefloxacin, 100.6%, RSD = 3.6%, n = 3). The use of multivariate calibration was particularly useful for spectrophotometric quantification because of the highly overlapping spectra of the binary mixture.
authors.affiliation: Department of Pharmaceutical Science, Florence University, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Gratteri
authors.forename: G
authors.initials: G
authors.lastname: Cruciani
mesh_terms: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000097:blood*; D002138:Calibration; D004359:Drug Therapy, Combination / Q000097:blood; D006801:Humans; D008795:Metronidazole / Q000097:blood*; D015366:Pefloxacin / Q000097:blood*; D011048:Polarography; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D008795:Metronidazole; D015366:Pefloxacin