title: Structural analyses of new tri- and tetrasaccharides produced from disaccharides by transglycosylation of purified Trichoderma viride beta-glucosidase.
abstract: A new beta-glucosidase was partially purified from Trichoderma viride cellulase. This beta-glucosidase catalyzed a transglycosylation reaction of cellobiose to give beta-D-Glc-(1-->6)-beta-D-Glc-(1-->4)-D-Glc (1, yield: 18.8%) and beta-D-Glc-(1-->6)-beta-D-Glc-(1-->6)-beta-D-Glc-(1-->4)-D-Glc (2, 3.7%), regioselectively. Furthermore, the enzyme regioselectively converted laminaribiose and gentiobiose into beta-D-Glc-(1-->6)-beta-D-Glc-(1-->3)-D-Glc (3, 15.3%) and beta-D-Glc-(1-->6)-beta-D-Glc-(1-->6)-D-Glc (4, 20.2%), respectively. The structures (1-4) of the products were determined by 1H and 13C NMR spectroscopies. This high regio- and stereoselectively of the beta-glucosidase could be applied for oligosaccharide synthesis.
authors.affiliation: Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan. kohno@dove-mc.eng.hokudai.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Kono
authors.forename: S
authors.initials: S
authors.lastname: Kawano
authors.forename: K
authors.initials: K
authors.lastname: Tajima
authors.forename: T
authors.initials: T
authors.lastname: Erata
authors.forename: M
authors.initials: M
authors.lastname: Takai
mesh_terms: D002240:Carbohydrate Sequence; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D013379:Substrate Specificity; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology*; D001617:beta-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D001617:beta-Glucosidase