[Effect of vasectomy on apoptosis in spermatogenic cells of the male rat].
To explore the effect of vasectomy on apoptosis of spermatogenic cells, we have detected apoptosis in the testis of vasectomized and sham-operated adult rats within a period of 12 postoperative weeks by means of flow cytometry and TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuride triphosphate nick-end labeling) method. Flow cytometry found that, in vasectomized rat, the sub-haploid peak was higher and the percentage of cells with sub-haploid was increased. The total number of positive TUNEL cells in the vasectomized group was greater than that in the sham-operated group (P < 0.001). Compared with the same time-phased sham-operated subgroups, the number of apoptotic spermatogonia and primary spermatocytes in the testes of the subgroups 2 weeks, 6 weeks and 10 weeks after vasectomy as well as the number of spermatogonia of the subgroup 12 weeks after vasectomy were significantly higher (P < 0.05). These findings demonstrate that vasectomy could enhance the apoptosis of spermatogenic cells.
Shen
K
K
Department of Urology, First Affiliated Hospital, Chengdu.
Yang
Y
Y
Tang
X
X
Huang
M
M
Li
H
H
Shen
H
H
Lei
S
S
Wei
Y
Y
Zhang
H
H
D000818:Animals; D017209:Apoptosis; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013090:Spermatocytes / Q000166:cytology*; D013093:Spermatogonia / Q000166:cytology*; D014659:Vasectomy / Q000009:adverse effects*