title: Las21 participates in extracellular/cell surface phenomena in Saccharomyces cerevisiae.
abstract: Las21 (Yj1062W) is a member of the major facilitator super family, possessing multimembrane spanning domains. The LAS21 gene was identified as a responsible gene for a Saccharomyces cerevisiae mutan which shows sensitivity to a local anestheticum, tetracaine. The null las21 mutant (las21 delta) is viable but shows temperature sensitive growth. We found, in addition to this phenotype, that the las21 delta strain shows a number of defects; mating deficiency, calcofluor resistance, and formation of Zymolyase sensitive spores. Temperature sensitive growth of the las21 delta mutant was found to be suppressed by 0.1 M MgSO4. Two multicopy suppressors were obtained. They are ECM33 (YBR078W) and PIR2/HSP150 (YJR159W) both have some roles in an extracellular function. The common features of the suppressors, genetic and physiological, of the las21 delta mutation suggest that Las21 participates in a global activity of extracellular phenomena. The las 21 phenotypes are consistent with the idea that Las21/Gpi7 acts in metabolism of glycosylphosphatidylinositol.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo.
authors.forename: A
authors.initials: A
authors.lastname: Tohe
authors.forename: T
authors.initials: T
authors.lastname: Oguchi
mesh_terms: D001557:Benzenesulfonates / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D017261:Glycosylphosphatidylinositols / Q000502:physiology; D006867:Hydrolases / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D013172:Spores, Fungal
chemical_list: D001557:Benzenesulfonates; D005656:Fungal Proteins; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; C037833:zymolyase; C007061:C.I. Fluorescent Brightening Agent 28; D006867:Hydrolases; D008274:Magnesium