title: [Effect of various methods of immobilization on stability of a microbial biosensor based on Pseudomonas rathonis T during detection of surfactants].
abstract: The operating and storage stability of a receptor element of an amperometric biosensor based on the Pseudomonas rathonis strain T capable of degrading surfactants was tested. Microbial cells were immobilized by incorporation in gels (agar, agarose, and calcium-alginate), polyvinyl alcohol membrane, adhesion to the chromatographic paper GF/A, or by the cross-linking induced by glutaric aldehyde. Incorporation of microbial cells in agar gel provides the long-standing conservation of their activity and viability during measurements of high concentrations of surfactants and allows the receptory element of the biosensor to be rapidly recovered after the measurements.
authors.affiliation: Dumanskii Institute of Colloid Chemistry and Chemistry of Water, National Academy of Sciences of Ukraine, Kiev, Ukraine.
authors.forename: I N
authors.initials: IN
authors.lastname: Semenchuk
authors.forename: L A
authors.initials: LA
authors.lastname: Taranova
authors.forename: A A
authors.initials: AA
authors.lastname: Kaleniuk
authors.forename: P V
authors.initials: PV
authors.lastname: Il'iasov
authors.forename: A N
authors.initials: AN
authors.lastname: Reshetilov
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D018914:Cells, Immobilized; D011549:Pseudomonas; D013501:Surface-Active Agents / Q000032:analysis*
chemical_list: D013501:Surface-Active Agents