title: Replicative forms of TT virus DNA in bone marrow cells.
abstract: TT virus (TTV) is a human virus consisting of a single-stranded, circular DNA genome of 3.8 kilobases (kb). To examine whether TTV replicates in peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BMCs), DNA was extracted from the PBMCs and/or BMCs of six TTV-infected individuals and separated by agarose gel electrophoresis. The TTV DNAs from the PBMCs migrated to the 2.0- to 2.5-kb region. The TTV DNAs from the BMCs migrated to the 2.0- to 2. 5-kb and 3.3- to 6.1-kb regions. The faster-migrating TTV DNAs were sensitive to S1 nuclease, while the slower-migrating TTV DNAs were resistant and their position on the agarose gel shifted to the position of the full genomic size upon digestion with restriction enzyme PstI. Full-length inverted polymerase chain reaction on the slower-migrating, double-stranded TTV DNAs from the BMCs amplified a 3.8-kb product. Replicative intermediate forms of TTV DNA are present in BMCs but not in PBMCs.
authors.affiliation: Immunology Division and Division of Molecular Virology, Tochigi-Ken, 329-0498, Japan. immunodiv@jichi.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Nishizawa
authors.forename: A
authors.initials: A
authors.lastname: Tawara
authors.forename: Y
authors.initials: Y
authors.lastname: Sugai
authors.forename: T
authors.initials: T
authors.lastname: Sai
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: F
authors.initials: F
authors.lastname: Tsuda
mesh_terms: D001483:Base Sequence; D001854:Bone Marrow Cells / Q000821:virology*; D017931:DNA Primers; D004261:DNA Replication; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004279:DNA, Viral