title: Myelin glycosphingolipid/cholesterol-enriched microdomains selectively sequester the non-compact myelin proteins CNP and MOG.
abstract: Plasma membranes are complex arrays of protein and lipid subdomains. Detergent-insoluble, glycosphingolipid/cholesterol-enriched micro-domains (DIGCEMs) have been implicated in protein sorting and/or as sites for signaling cascades in the plasma membrane. We previously identified the presence of DIGCEMs in oligodendrocytes in culture and purified myelin and characterized a novel DIGCEM-associated tetraspan protein, MVP17/rMAL (Kim et al. (1995) Journal of Neuroscience Research 42, 413-422). We have now analyzed the association of known myelin proteins with DIGCEMs in order to provide a better understanding of their roles during myelin biogenesis. We used four well-established criteria to identify myelin DIGCEM-associated proteins: insolubility in a non-ionic detergent Triton X-100 at low temperature (4 degrees C), flotation of the insoluble complexes to low density fractions in sucrose gradients, and TX-100 solubilization at 37 degrees C, or at 4 degrees C following treatment with the cholesterol-binding detergent saponin. We demonstrate that these proteins fall into four distinct groups. Although all tested proteins could be floated to a low-density fraction, proteolipid protein (PLP), myelin basic protein (MBP) and myelin associated glycoprotein (MAG) were solubilized by the detergent extraction, and connexin32 (Cx32) and oligodendrocyte-specific protein (OSP) met only some of the criteria for DIGCEMs. Only the non-compact myelin proteins 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and myelin/oligodendrocyte glycoprotein (MOG) satisfied all four criteria for DIGCEM-associated proteins. Significantly, only approximately 40% of CNP and MOG were selectively associated with DIGCEMs. This suggests that they may have both non-active "soluble", and functionally active DIGCEM-associated, forms in the membrane, consistent with current views that DIGCEMs provide platforms for bringing together and activating components of the signal transduction apparatus. We therefore propose that CNP and MOG may have unique roles among the major myelin proteins in signaling pathways mediated by lipid-protein microdomains formed in myelin.
authors.affiliation: Department of Microbiology, MC-3205, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3205, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kim
authors.forename: S E
authors.initials: SE
authors.lastname: Pfeiffer
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001923:Brain Chemistry / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism*; D017630:Connexins / Q000032:analysis / Q000378:metabolism; D004676:Myelin Basic Protein / Q000032:analysis / Q000378:metabolism; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein / Q000032:analysis / Q000378:metabolism; D009186:Myelin Sheath / Q000737:chemistry / Q000201:enzymology*; D018992:Myelin-Associated Glycoprotein / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D063308:Myelin-Oligodendrocyte Glycoprotein; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D013347:Subcellular Fractions / Q000737:chemistry / Q000201:enzymology; D000097002:Gap Junction beta-1 Protein
chemical_list: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D000911:Antibodies, Monoclonal; D002784:Cholesterol; D017630:Connexins; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D000097002:Gap Junction beta-1 Protein; C571328:Mog protein, rat