Activity of some 3-formylchromone derivatives on the induction of chloroplast-free mutants in Euglena gracilis.
The hereditary bleaching test on Euglena gracilis was used for detecting extranuclear mutations. The highest bleaching activity (induction of the chloroplast-free mutants) was shown by the 6-R-3-formylchromones. On the other hand, bleaching-inactive 6-R-3-formylchromone acylhydrazones (derived from gallic and salicylic acids), added at sufficient concentrations in the case of chloroplast mutagenesis in E. gracilis, act as a potent antimutagen. This effect appeared to be a unique feature of chromone derivatives, but was dependent on the type of mutagen. These substances were very effective against the bleaching activity of acridine orange, and were less effective against N-methyl-N'-nitro-N-nitrosoguanidine. The genotoxic effects of these mutagens was reduced, especially during the first stages of induction of this specific cytoplasmic mutation. The experimental study of mutagenicity and antimutagenicty of 3-formylchromone hydrazones was reinforced by data obtained by the semi-empirical AM1 method and lipophilicity values.
Foltínová
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P
Institute of Cell Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic.
Lácová
M
M
Loos
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D
D000818:Animals; D002867:Chromones / Q000737:chemistry / Q000494:pharmacology*; D005056:Euglena gracilis / Q000187:drug effects* / Q000235:genetics; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D009154:Mutation
D002867:Chromones; D009153:Mutagens; D008769:Methylnitronitrosoguanidine