title: In vivo gene gun-mediated DNA delivery into rodent brain tissue.
abstract: Various types of gene transfer into live tissues have been tried. However, in vivo gene transfer into brain tissue or neuronal cells without virus vector has required a great effort. Particle-mediated gene transfer into live brain tissue was thought to be impossible because of its fragility and the mechanical problem of a previous type of gene gun. In addition, particle-mediated DNA transfer into monolayer-cultured cells without mechanical damage has been difficult. We successfully transferred DNA into rodent live brain tissue and also into monolayer-cultured cells without mechanical damage by using a new type of gene gun and also confirmed gene expression in the brain. This new method represents another variation of gene transfer into the brain.
authors.affiliation: Department of Neurosurgery, Yokohama City University School of Medicine, Fukuura 3-9, Kanazawa-ku, Yokohama, 236-0004, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Hattori
authors.forename: S
authors.initials: S
authors.lastname: Kawamoto
authors.forename: I
authors.initials: I
authors.lastname: Kudoh
authors.forename: A
authors.initials: A
authors.lastname: Hayashi
authors.forename: I
authors.initials: I
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Yoshinari
authors.forename: M
authors.initials: M
authors.lastname: Minami
authors.forename: H
authors.initials: H
authors.lastname: Kanno
mesh_terms: D000818:Animals; D019470:Biolistics / Q000295:instrumentation*; D001921:Brain / Q000601:surgery / Q000648:ultrastructure; D002478:Cells, Cultured; D015870:Gene Expression; D005822:Genetic Vectors; D006046:Gold; D007763:Lac Operon; D051379:Mice; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D006046:Gold; D001616:beta-Galactosidase