Colocalization of aldehyde dehydrogenases and Fe/NADPH-induced lipid peroxidation in tissue sections of rat retina.
Epidemiological and experimental studies suggest the involvement of lipid peroxidation (LPO) in retinal diseases. Clinicians usually prescribe antioxidants to help in the treatment of proliferative diabetic vitreoretinopathy and age-related macular degeneration. In spite of this, these processes inexorably induce visual impairment and may progress towards blindness. In addition to other pathogenic mechanisms not fully understood, it may be that peroxidic aldehydes from LPO occurring in the eyes, acting as cytotoxic chemicals, mediate in these chronic disorders. To test the mechanisms of removing peroxidic aldehydes from retinal cells and in an attempt to understand long-lasting changes induced by LPO, the distribution and activity of aldehyde dehydrogenases (ALDH) in the rat retina were studied and compared with the LPO sites induced by iron/nicotine adenine dinucleotide phosphate. Histochemical and immunocytochemical assays revealed the colocalization of LPO and ALDH, mainly in the photoreceptors and inner retinal layers. This suggests the involvement of ALDH in detoxifying peroxidic aldehydes from the retina. Any change in ALDH retinal expression and distribution might be of crucial importance in assessing the paths of LPO-mediated vitreoretinopathies. Further research is needed to evaluate these findings and their application to new ophthalmic therapy.
Pinazo-Durán
M D
MD
Centro de Investigación, Hospital Universitario La Fé, Valencia, España. pinaaoduran@retemail.es
Verdejo
C
C
Azorín
I
I
Renau-Piqueras
J
J
Iborra
F J
FJ
D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000818:Animals; D019084:Fluorescent Antibody Technique, Indirect; D007150:Immunohistochemistry; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D009249:NADP / Q000494:pharmacology*; D051381:Rats; D012160:Retina / Q000201:enzymology* / Q000648:ultrastructure
D008054:Lipid Peroxides; D009249:NADP; D007501:Iron; D000444:Aldehyde Dehydrogenase