Effect of vitamin E on aspirin-induced gastric mucosal injury in rats.
We investigated the effect of vitamin E on aspirin-induced gastric mucosal injury in rats. Twenty-eight male Sprague-Dawley rats were divided into four groups and were fed for 20 weeks with a diet containing <0.1 mg/100 g of alpha-tocopherol (vitamin E-deficient), 2 mg/100 g of alpha-tocopherol (normal and vitamin E-sufficient), or 50 mg/100 g of alpha-tocopherol (vitamin E-supplemented). In vitamin E-deficient rats, oral administration of aspirin (200 mg/kg) plus HCI created more severe hemorrhagic erosions than in other rats. Vitamin E-deficient rats had higher levels of thiobarbituric acid reactive substances, myeloperoxidase activity, and cytokine-induced neutrophil chemoattractant in the gastric mucosa. Flow cytometry showed that CD18 expression on stimulated neutrophils was higher in vitamin E-deficient rats than in vitamin E-supplemented rats. These results suggest that vitamin E protects against aspirin-induced gastric mucosal injury by inhibiting lipid peroxidation and accumulation of activated neutrophils.
Sugimoto
N
N
Kyoto Prefectural University of Medicine, First Department of Internal Medicine, Japan.
Yoshida
N
N
Yoshikawa
T
T
Nakamuara
Y
Y
Ichikawa
H
H
Naito
Y
Y
Kondo
M
M
D000818:Animals; D001241:Aspirin / Q000009:adverse effects*; D002630:Chemotactic Factors / Q000032:analysis; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013858:Thiobarbiturates / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000473:pathology
D002630:Chemotactic Factors; D013858:Thiobarbiturates; D014810:Vitamin E; D009195:Peroxidase; D001241:Aspirin