title: Identification of a methylated tea catechin as an inhibitor of degranulation in human basophilic KU812 cells.
abstract: We examined the constituents of tea that had an inhibitory effect on the degranulation process in the human basophilic cell line, KU812. Among the constituents purified from a extract of 'Benihomare' oolong tea by column chromatography, a methylated (-)-epigallocatechin gallate ((-)-epigallocatechin 3-O-(3-O-methyl) gallate) was found to inhibit the degranulation of KU812 cells that had been stimulated with calcium ionophore A23187. The inhibitory effect of this methylated (-)-epigallocatechin gallate on degranulation was greater than that of (-)-epigallocatechin gallate. This result indicates that methylation of (-)-epigallocatechin gallate may be an effective modification for the catechin to inhibit degranulation from human basophils.
authors.affiliation: Division of Bioresources and Bioenvironmental Science, Graduate School, Kyushu University, Fukuoka, Japan. tatibana@agr.kyushu-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Tachibana
authors.forename: Y
authors.initials: Y
authors.lastname: Sunada
authors.forename: T
authors.initials: T
authors.lastname: Miyase
authors.forename: M
authors.initials: M
authors.lastname: Sano
authors.forename: M
authors.initials: M
authors.lastname: Maeda-Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Yamada
mesh_terms: D001491:Basophils / Q000166:cytology / Q000187:drug effects*; D002392:Catechin / Q000378:metabolism / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D008745:Methylation
chemical_list: D002392:Catechin