Monoclonal antibody to human sperm acrosomal protein.
A new monoclonal antibody designated Hs-14 was generated after immunization of BALB/c mice with the acid extract of human sperm. In indirect immunofluorescence Hs-14 mAb binds to the acrosome of permeabilized sperm cells and consequently recognizes some intra-acrosomal protein. Western blotting analysis revealed that under non-reducing conditions the Hs-14 mAb detects a protein with a molecular mass of 220 kDa. Under reducing conditions the Hs-14 recognizes several peptide bands within the range from 55 kDa to 110 kDa. Beside human sperm the antibody positively reacts also with sperm of some other mammalian species. Using Hs-14 mAb it is possible to evaluate the acrosomal integrity of spermatozoa and to reveal sperm pathology.
Capková
J
J
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. capkova@biomed.cas.cz
Geussová
G
G
Peknicová
J
J
D000177:Acrosome / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000276:immunology*; D013045:Species Specificity; D013094:Spermatozoa / Q000276:immunology*; D013552:Swine
D000911:Antibodies, Monoclonal; D011506:Proteins