title: Ultrastructural and immunohistochemical study of basal lamina of the testis in adolescent varicocele.
abstract: OBJECTIVE
  To evaluate a possible involvement of the extracellular matrix (ECM) in the testes of adolescents with varicocele.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  University-based medical center.
  
  
  METHODS
  Twenty-four adolescents aged between 13 and 18 years underwent surgical treatment for repair of left idiopathic varicocele.
  
  
  METHODS
  A testis biopsy was performed at time of surgery.
  
  
  METHODS
  Transmission electron microscopy study of basal lamina and immunofluorescence studies of collagen type IV and laminin, two major components of basal lamina.
  
  
  RESULTS
  Transmission electron microscopy observations showed an uneven profile of the basal lamina with a variable thickness. Immunofluorescence studies showed an irregular immunofluorescent line that appeared interrupted in some observations. Collagen type IV showed some areas of strong immunostaining with other areas with reduced immunoreactivity.
  
  
  CONCLUSIONS
  Our ultrastructural and immunohistochemical observations highlight focal damage at the level of peritubular basal lamina, but this damage is not as severe as that described in adult varicocele. Initial involvement of basal lamina could represent one of the mechanisms responsible for varicocele-induced histologic alterations of the testes.
authors.affiliation: Faculty of Medicine and Surgery, University of Messina, Messina, Italy. santorof@unime.it
authors.forename: G
authors.initials: G
authors.lastname: Santoro
authors.forename: C
authors.initials: C
authors.lastname: Romeo
authors.forename: P
authors.initials: P
authors.lastname: Impellizzeri
authors.forename: C
authors.initials: C
authors.lastname: Gentile
authors.forename: G
authors.initials: G
authors.lastname: Anastasi
authors.forename: A
authors.initials: A
authors.lastname: Santoro
mesh_terms: D000293:Adolescent; D003094:Collagen / Q000378:metabolism / Q000648:ultrastructure; D003430:Cross-Sectional Studies; D005109:Extracellular Matrix / Q000473:pathology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D013737:Testis / Q000473:pathology* / Q000648:ultrastructure*; D014646:Varicocele / Q000473:pathology*
chemical_list: D007797:Laminin; D003094:Collagen