title: Isolation and identification of the probing stimulants in the rice plant for the white-back planthopper, Sogatella furcifera (homoptera: delphacidae).
abstract: Adult females of the white-back planthopper, Sogatella furcifera, showed characteristic behavior of stylet sheath deposit on a parafilm membrane when fed on a 2% aqueous crude rice leaf and stem extract containing 15% sucrose. Subsequent bioassays revealed that the butanol-soluble fraction of the extract was highly active against the insects. When the butanol fraction was chromatographed on an ODS open column and eluted in sequence with a mixture of an increasing concentration of methanol in water, the 40 % methanol fraction was separated as the most active. A further bioassay of the HPLC components in the active fraction revealed that two major components (1 and 3) stimulated the high probing activity of the white-back planthopper only when they were combined. Of the active components, one component (3) was identified to be tricin 5-O-glucoside by spectroscopic analyses.
authors.affiliation: Department of Bioresources Science, Faculty of Agriculture, Kochi University, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Adjei-Afriyie
authors.forename: C S
authors.initials: CS
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Takemura
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Horiike
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007313:Insecta / Q000502:physiology*; D007605:Juvenile Hormones / Q000737:chemistry*; D013058:Mass Spectrometry; D012275:Oryza / Q000737:chemistry*
chemical_list: D007605:Juvenile Hormones