Myeloperoxidase-catalyzed formation of PCDD/F from chlorophenols.
Chlorophenols (CP) are transformed in vitro to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) by a biochemical-catalyzed oxidation. This is shown for 2,4,5-tri-, 2,3,4,6-tetra-and pentachlorophenol with myeloperoxidase recovered from human leucocytes in the presence of hydrogen peroxide. The yield, the reaction, and the PCDD/F-pattern found depend on the CP. The formation rates are in the micromol-per-mol range for all substrates. The experiments confirm the suspicion that a biochemical formation of PCDD/F from precursors such as CPs can take place in the human body and that this metabolic pathway may lead to a higher inner exposure with PCDD/F than is now assumed.
Wittsiepe
J
J
Ruhr-Universität Bochum, Abteilung für Hygiene, Sozial- und Umweltmedizin, Germany. wittsiepe@hygiene.ruhr-uni-bochum.de
Kullmann
Y
Y
Schrey
P
P
Selenka
F
F
Wilhelm
M
M
D001572:Benzofurans / Q000378:metabolism*; D002733:Chlorophenols / Q000378:metabolism*; D000072338:Dibenzofurans, Polychlorinated; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000378:metabolism
D001572:Benzofurans; D002733:Chlorophenols; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D009195:Peroxidase