title: Chromosomal diagnosis in each individual blastomere of 5- to 10-cell bovine embryos derived from in vitro fertilization.
abstract: Chromosomal normality and sex were diagnosed in each blastomere of bovine embryos derived from in vitro fertilization (IVF). Bovine embryos developing to the 5- to 10-cell stage were separated into individual blastomeres with 0.5% protease. After treatment with 100 ng/mL vinblastine sulfate for 8 to 10 h, they were prepared for chromosome samples. In total, 33 bovine embryos and 185 blastomeres were examined. Chromosomal normality was analyzed in 43.8% (81/185) of the blastomeres and 60.6% (20/33) of the embryos; while chromosomal anomalies were found in 16 (80%, 16/20) of the embryos, 5 haploid embryos and 11 mosaic (n/2n) embryos. Mosaicism characteristic of the opposite sex in X-and Y-chromosomes was found in 2 haploid embryos, and that of a Y-chromosome and of XX chromosomes in 1 n/2n embryo. Various sex-chromosome compositions were also observed in the other 10 chromosomal mosaic n/2n embryos.
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshizawa
authors.forename: H
authors.initials: H
authors.lastname: Konno
authors.forename: S
authors.initials: S
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Kageyama
authors.forename: E
authors.initials: E
authors.lastname: Fukui
authors.forename: S
authors.initials: S
authors.lastname: Muramatsu
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Araki
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000648:ultrastructure*; D002417:Cattle / Q000196:embryology*; D002869:Chromosome Aberrations; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D006238:Haploidy; D009030:Mosaicism; D019836:Preimplantation Diagnosis / Q000662:veterinary*; D012730:Sex Chromosomes