Effect of mare's age and recovery methods on the recovery rate of equine follicular oocytes for IVM procedures.
Mares (n = 39) were classified according to age as young (less than 1.5 yr, n = 17) or old (more than 1.5 yr, n = 22) and sacrificed. Ovaries were measured and weighed, and the number of follicles and CL were counted. Follicle size and distribution were recorded (external: > 20 mm, < 20 mm; internal: > 5 mm, < 5 mm). External follicles were aspirated while internal follicles were sliced. The number and Type of oocytes recovered using each method were recorded. Oocyte recovery rates (oocytes/ovary) resulted in a mean of 0.92 oocytes by aspiration and 1.36 oocytes by additional slicing. The mean numbers of available follicles (8.11 and 5.02) oocytes recovered (4.94 and 3.02) and oocytes selected per ovary (3.29 and 2.32) were not significantly different in the young or old mares, respectively.
Erice
I
I
Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Spain.
Gil
L
L
Josa
A
A
Echegaray
A
A
Martinez
F
F
Espinosa
E
E
D000818:Animals; D002469:Cell Separation / Q000379:methods; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D006736:Horses; D066298:In Vitro Techniques; D001239:Inhalation; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D009866:Oogenesis / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology*; D010053:Ovary / Q000033:anatomy & histology / Q000502:physiology