title: Evaluation of the postprandial effects of a fast-food meal on human plasma F(2)-isoprostane levels.
abstract: Measurement of the F(2)-isoprostane, 8-epi-PGF(2alpha) is increasingly used as a sensitive and reliable marker of lipid peroxidation in vivo. Because the majority of 8-epi-PGF(2alpha) in plasma is associated with lipoproteins, it is possible that 8-epi-PGF(2alpha) derived from polyunsaturated fatty acid-rich food may become incorporated within these lipoproteins during synthesis and could contribute to the levels detected in plasma. In this study, we evaluated the postprandial effect of a single fast-food meal (McDonald's Big Mac meal, McDonald's Corp., London, England) on plasma total 8-epi-PGF(2alpha) in nine healthy subjects. Blood was collected before and 2 h postprandially. 8-Epi-PGF(2alpha) was measured by immunoaffinity extraction and gas chromatography-mass spectrometry. Fasting plasma 8-epi-PGF(2alpha) (875 +/- 25 pM) increased postprandially (956 +/- 23 pM, p <.05), although no significant change was observed in the normalized concentrations (2. 78 +/- 0.1 vs. 2.95 +/- 0.3 nmol/mmol arachidonic acid). Plasma lipid hydroperoxides, fatty acids, vitamin E, total antioxidant status, cholesterol, and triglycerides were not altered. Plasma glucose increased postmeal (4.4 +/- 0.1 vs. 4.9 +/- 0.1 mM, p <.05). These results indicate that the overall contribution of this lipid-rich meal to plasma 8-epi-PGF(2alpha) and other lipid peroxidation markers was small.
authors.affiliation: The William Harvey Research Institute, St. Bartholomew's and The Royal London School of Medicine and Dentistry, London, England, United Kingdom. n.k.gopaul@mds.qmw.ac.uk
authors.forename: N K
authors.initials: NK
authors.lastname: Gopaul
authors.forename: K
authors.initials: K
authors.lastname: Zacharowski
authors.forename: B
authors.initials: B
authors.lastname: Halliwell
authors.forename: E E
authors.initials: EE
authors.lastname: Anggård
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D016718:Arachidonic Acid / Q000097:blood; D001161:Arteriosclerosis / Q000209:etiology; D015415:Biomarkers; D004041:Dietary Fats / Q000008:administration & dosage* / Q000009:adverse effects; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D004435:Eating / Q000502:physiology*; D028441:F2-Isoprostanes; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004041:Dietary Fats; D028441:F2-Isoprostanes; C075750:8-epi-prostaglandin F2alpha; D016718:Arachidonic Acid; D015237:Dinoprost