The influence of enucleation on the ultrastructure of in vitro matured and enucleated cattle oocytes.
The enucleation of recipient oocytes in nuclear transfer experiments is generally carried out by aspirating one third of the ooplasm adjacent to the first polar body. It was supposed that this enucleation step affects the ultrastructure of the remaining cytoplast, resulting in a decline or destruction of its cellular compartments. Even if the transferred nucleus had the potential to support the development of a single-cell nucleus transfer embryo to the blastocyst stage, meiotic division could be stopped at any stage if the destruction of the ultrastructure of host cytoplasm resulted in a limited metabolism. The present study was conducted to investigate the influence of the enucleation procedure on the ultrastructure of the remaining ooplast. In vitro matured oocytes; in vitro matured and enucleated oocytes; and in vitro matured and enucleated oocytes that were subsequently cultivated in vitro for additional 4 h were prepared for transmission electron microscopy (TEM). An examination of ultra-thin sections showed that the arrangement of organelles in all matured oocytes was in accordance with that already described for normal oocyte development. Immediately after enucleation no major differences in the arrangement of cortical granules, mitochondria, smooth endoplasmic reticulum (SER), lipid droplets and vacuoles were found compared with nonmanipulated oocytes. After enucleation and 4 h of culture, 24- and 36-h matured oocytes differed from each other in the arrangement of large aggregates of SER surrounded by a wall of mitochondria and lipid droplets. These complexes were still found in the 24-h but not in 36-h matured, enucleated and cultivated oocytes. Clusters of SER, mitochondria and lipid droplets were described by different authors as having metabolic activity. The results of this study in connection with results from nuclear transfer experiments suggest that these aggregates and their metabolic activity can be transferred with cytoplasm from 24- but not 36-h matured oocytes. Only cytoplasm from the 24-h matured oocytes showed a development-supporting effect when fused to enucleated recipient cells before nuclear transfer.
Greising
T
T
Research Institute for the Biology of Farm Animals, Department for Reproductive Biology, Dummerstorf, Germany.
Jonas
L
L
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary; D018871:Endoplasmic Reticulum, Smooth / Q000648:ultrastructure; D005260:Female; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D009865:Oocytes / Q000166:cytology / Q000648:ultrastructure*
D008055:Lipids