Plasma membrane calcium ATPase gene expression in bovine lens epithelium.
Plasma membrane calcium adenosine triphosphatase (Ca(2+)-ATPase) is an energy-dependent protein responsible for transporting cytosolic calcium across the plasma membrane. Multiple plasma membrane Ca(2+)-ATPase isoforms are expressed from four genes (PMCA1-4) and alternative mRNA splicing. We have studied PMCA gene expression in bovine lens epithelium tissues by reverse transcription-polymerase chain reaction, Southern blot, and Northern blot hybridization. All four PMCA genes are expressed in the lens epithelium, the PMCA3 transcript being the most abundant. The transcripts for PMCA1, PMCA2, and PMCA4 exist in decreasing order of abundance. There is no evidence for the expression of any novel PMCA genes in bovine lens epithelium.
Bian
L
L
Department of Ophthalmology and Visual Sciences, University of Louisville School of Medicine, Louisville, KY 40202, USA.
Zeng
J
J
Borchman
D
D
Paterson
C A
CA
D000818:Animals; D015415:Biomarkers; D015152:Blotting, Northern; D015139:Blotting, Southern; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D017931:DNA Primers / Q000737:chemistry; D004848:Epithelium / Q000201:enzymology*; D015870:Gene Expression; D007903:Lens Capsule, Crystalline / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; D017931:DNA Primers; D012333:RNA, Messenger; D000252:Calcium-Transporting ATPases