[Effect of various methods of immobilization on stability of a microbial biosensor based on Pseudomonas rathonis T during detection of surfactants].
The operating and storage stability of a receptor element of an amperometric biosensor based on the Pseudomonas rathonis strain T capable of degrading surfactants was tested. Microbial cells were immobilized by incorporation in gels (agar, agarose, and calcium-alginate), polyvinyl alcohol membrane, adhesion to the chromatographic paper GF/A, or by the cross-linking induced by glutaric aldehyde. Incorporation of microbial cells in agar gel provides the long-standing conservation of their activity and viability during measurements of high concentrations of surfactants and allows the receptory element of the biosensor to be rapidly recovered after the measurements.
Semenchuk
I N
IN
Dumanskii Institute of Colloid Chemistry and Chemistry of Water, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Taranova
L A
LA
Kaleniuk
A A
AA
Il'iasov
P V
PV
Reshetilov
A N
AN
D015374:Biosensing Techniques / Q000379:methods*; D018914:Cells, Immobilized; D011549:Pseudomonas; D013501:Surface-Active Agents / Q000032:analysis*
D013501:Surface-Active Agents