Hemopexin as a carrier protein of tumor-localizing Ga-metalloporphyrin-ATN-2.
During size exclusion HPLC, ATN-2 binding protein separated from human and mouse sera, SCCVII and colon 26 tumor tissues were found in fraction 13 (A: estimated molecular weight 70,000). Fraction 13(A) of human sera was exclusively reactive to the human hemopexin antibody. During two-dimensional electrophoresis and amino acid sequence analysis, Fraction 13(A) of C3H/He mouse sera was found to have partial homology with the mouse hemopexin precursor. Glycoprotein with the same domain structure of hemopexin has been proposed to be an important carrier protein that forms the tumor-localizing activity of water-soluble porphyrin.
Nakajima
S
S
Division of Surgical Operation, Asahikawa Medical College, Japan. nakajima@asahikawa-med.ac.jp
Moriyama
T
T
Hayashi
H
H
Sakata
I
I
Nakae
Y
Y
Takemura
T
T
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006466:Hemopexin / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011166:Porphyrins / Q000032:analysis / Q000378:metabolism*
D002352:Carrier Proteins; D011166:Porphyrins; D006466:Hemopexin