Preventive effect of monoclonal antibodies to intercellular adhesion molecule-1 and leukocyte function-associate antigen-1 on murine spontaneous fetal resorption.
OBJECTIVE
Are cell adhesion molecules involved in the murine model of immunologically-mediated spontaneous abortion?


METHODS
Pregnant CBA/J female mice mated with DBA/2 male mice were injected with monoclonal antibodies (MAbs) to intercellular adhesion molecule-1 (ICAM-1) and leukocyte function-associate antigen-1 (LFA-1). On day 13 of gestation. viable and resorbed embryos were counted. Natural killer (NK) cell activity in the spleen, mixed lymphocyte reactions (MLR), mixed lymphocyte-placenta reactions (MLPR), and levels of interferon (IFN)-gamma were assayed.


RESULTS
Significant suppression of fetal resorption was observed by the injection of MAb to ICAM-1 and LFA-1. NK cell activity and the MLR anti-(CBA/J x DBA/ 2)F1 were reduced in the antibody-treated CBA/J spleen. Moreover, the level of IFN-gamma was significantly lower in the MLPR supernatants from the antibody-treated group than those of the control group.


CONCLUSIONS
One mechanism in the murine model of spontaneous abortion may be through the interaction of cell adhesion molecules, which may modulate NK cell activities and cytokine production.
Takeshita
T
T
Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo, Japan.
Satomi
M
M
Akira
S
S
Nakagawa
Y
Y
Takahashi
H
H
Araki
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000627:therapeutic use*; D002474:Cell-Free System / Q000276:immunology; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D010064:Embryo Implantation / Q000276:immunology; D005260:Female; D005327:Fetal Resorption / Q000276:immunology* / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000627:therapeutic use; D007274:Injections, Intraperitoneal; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D007371:Interferon-gamma / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007166:Immunosuppressive Agents; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma