title: Coping with replication 'train wrecks' in Escherichia coli using Pol V, Pol II and RecA proteins.
abstract: DNA replication machineries tend to stall when confronted with damaged DNA template sites, causing the biochemical equivalent of a major 'train wreck'. A newly discovered bacterial DNA polymerase, Escherichia coli Pol V, acting in conjunction with the RecA protein, can exchange places with the stalled replicative Pol III core and catalyse 'error-prone' translesion synthesis. In contrast to Pol V-catalysed 'brute-force, sloppier copying', another SOS-induced DNA polymerase, Pol II, plays a pivotal role in an 'error-free', replication-restart DNA repair pathway and probably involves RecA-mediated homologous recombination.
authors.affiliation: Dept of Biological Sciences and Chemistry, University of Southern California, University Park, Los Angeles, CA 90089-1340, USA. mgoodman@mizar.usc.edu
authors.forename: M F
authors.initials: MF
authors.lastname: Goodman
mesh_terms: D002384:Catalysis; D004257:DNA Polymerase II / Q000378:metabolism*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D016296:Mutagenesis; D013014:SOS Response, Genetics
chemical_list: D029968:Escherichia coli Proteins; D004257:DNA Polymerase II; C120926:DNA polymerase V, E coli; D004259:DNA-Directed DNA Polymerase