title: Two-state expansion and collapse of a polypeptide.
abstract: The initial phase of folding for many proteins is presumed to be the collapse of the polypeptide chain from expanded to compact, but still denatured, conformations. Theory and simulations suggest that this collapse may be a two-state transition, characterized by barrier-crossing kinetics, while the collapse of homopolymers and random heteropolymers is continuous and multi-phasic. A new rapid-mixing flow technique has been used to resolve the late stages of polypeptide collapse, at time scales >/=45 microseconds. We have used a laser temperature-jump with fluorescence spectroscopy to resolve the complete time-course of the collapse of denatured cytochrome c with nanosecond time resolution. We find the process to be exponential in time and thermally activated, with an apparent activation energy approximately 9 k(B)T (after correction for solvent viscosity). These results indicate that polypeptide collapse is kinetically a two-state transition. Because of the observed free energy barrier, the time scale of polypeptide collapse is dramatically slower than is predicted by Langevin models for homopolymer collapse.
authors.affiliation: Laboratory of Chemical Physics, NIDDK National Institutes of Health, Building 5, Bethesda, MD, 20892-0520, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Hagen
authors.forename: W A
authors.initials: WA
authors.lastname: Eaton
mesh_terms: D000818:Animals; D003198:Computer Simulation; D003574:Cytochrome c Group / Q000737:chemistry* / Q000378:metabolism*; D004058:Diffusion; D019791:Guanidine / Q000494:pharmacology; D006418:Heme / Q000378:metabolism; D006736:Horses; D007700:Kinetics; D007834:Lasers; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding; D012997:Solvents; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013816:Thermodynamics; D014364:Tryptophan / Q000378:metabolism; D014783:Viscosity
chemical_list: D003574:Cytochrome c Group; D010455:Peptides; D012997:Solvents; D006418:Heme; D014364:Tryptophan; D019791:Guanidine