title: The SCAN domain mediates selective oligomerization.
abstract: The SCAN domain is described as a highly conserved, leucine-rich motif of approximately 60 amino acids found at the amino-terminal end of zinc finger transcription factors. Although no specific biological function has been attributed to the SCAN domain, its predicted amphipathic secondary structure led to the suggestion that this domain may mediate protein-protein associations. A yeast two-hybrid screen identified members of two SCAN domain protein families that interact with the SCAN domain of the zinc finger protein ZNF202. The interacting ZNF191 protein represents the family of SCAN domain-containing zinc finger proteins, whereas the novel SDP1 protein establishes a new family of genes that encode an isolated SCAN domain. Isolated SCAN domain proteins may form asymmetric homodimers in solution. Biochemical binding studies confirmed the associations of ZNF191 and SDP1 with ZNF202 and established the SCAN domain as a selective hetero- and homotypic oligomerization domain. SCAN mediated protein associations might therefore represent a new regulatory mechanism of transcriptional activity.
authors.affiliation: Novartis Institute for Biomedical Research, Summit, New Jersey 07901 and Myriad Genetics, Salt Lake City, Utah 84108, USA. schumacher@pharma.novartis.com
authors.forename: C
authors.initials: C
authors.lastname: Schumacher
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Honer
authors.forename: W
authors.initials: W
authors.lastname: Ding
authors.forename: J
authors.initials: J
authors.lastname: Koehn
authors.forename: Q
authors.initials: Q
authors.lastname: Lawrence
authors.forename: C M
authors.initials: CM
authors.lastname: Coulis
authors.forename: L L
authors.initials: LL
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Ballinger
authors.forename: B R
authors.initials: BR
authors.lastname: Bowen
authors.forename: S
authors.initials: S
authors.lastname: Wagner
mesh_terms: D000595:Amino Acid Sequence; D001704:Biopolymers; D002460:Cell Line; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
chemical_list: D001704:Biopolymers; D047908:Intracellular Signaling Peptides and Proteins; C408468:SCAND1 protein, human; D015534:Trans-Activators; D014157:Transcription Factors