Evaluation of the cytotoxicity of calcium phosphate root canal sealers by MTT assay.
The purpose of this study was to evaluate the cytotoxicity of some calcium phosphate-based sealers (Sankin apatite root canal sealers (SARCS) types 1 to 3) in comparison with currently used sealers (CRCS, Ketac Endo, AH26, and Endomethasone) by using MTT assay on L929 cells. Monolayer cell cultures were prepared on 96-well plates. After incubation at 37 degrees C in a humidified 5% CO2-containing air atmosphere for 24 h in the presence of each sealer extracts, 25 microliters of 5 mg/ml of MTT in saline were added into each well and incubated a further 3 h at 37 degrees C. A solubilization buffer consisting of 23% sodium dodecyl sulfate in 50% N,N-dimethylformamide (pH 4.7) was used to dissolve formazan precipitate. The optical densities of the plates were then read by a microplate spectrophotometer at 570 nm. Greater magnitude of optical density due to intense blue coloring is regarded as showing a higher percentage of cell viability. Among the different types of sealers, SARCS types 1 to 3 and CRCS did not exert any cytotoxic effects, whereas AH26, Ketac Endo, and Endomethasone produced some cytotoxicity.
Telli
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Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
Serper
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Dogan
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Guc
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D000818:Animals; D002130:Calcium Phosphates / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005562:Formazans; D007739:L Cells / Q000187:drug effects; D016014:Linear Models; D051379:Mice; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013778:Tetrazolium Salts; D018675:Toxicity Tests
D002130:Calcium Phosphates; D005562:Formazans; D012387:Root Canal Filling Materials; D013778:Tetrazolium Salts; C079782:MTT formazan