Expression of protein kinase C genes in normal (+/+) and W mutant alleles (Wsh/Wsh, W/Wv) mice testes.
In the present study, we investigated the expression of mRNA of protein kinase C (PKC) isoenzymes (alpha, beta, gamma, delta, epsilon, zeta, eta, and theta) in normal (+/+) and W mutant alleles mice testes. In +/+ mice testes, abundant expression of PKCdelta and PKCtheta was observed, while other PKCs (alpha, beta, gamma, epsilon, zeta, and eta) generally were not detected by Northern blotting. The PKCdelta and PKCtheta isoenzymes demonstrated a distinctive cellular distribution when evaluated by in situ hybridization. We have previously shown that PKCdelta gene was selectively expressed in spermatid of +/+ testes. Here we show that PKCdelta gene is also present in spermatid of Wsh/Wsh mice testes and PKCtheta gene was present in interstitial cells of +/+, Wsh/Wsh, and W/Wv mice testes. These studies provide the evidence of selective cell distributions of the PKC isoenzymes and suggest that PKC has the functional significance in testes.
Kim
H M
HM
Department of Oriental Pharmacy, College of Pharmacy, Center of Oriental Medicinal Science, Iksan, Chonbuk, South Korea.
Jin
E
E
Park
S T
ST
Kim
J J
JJ
Yoon
H S
HS
Oh
Y K
YK
Oh
K S
KS
Chung
Y T
YT
D000483:Alleles; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D005838:Genotype; D005854:Germ Cells / Q000502:physiology; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D013737:Testis / Q000378:metabolism*
D007527:Isoenzymes; D012333:RNA, Messenger; D011493:Protein Kinase C