Suppression of heat-induced HSP-70 by simultaneous exposure to 50 mT magnetic field.
Effect of extremely low frequency magnetic field (ELFMF) at 50 mT and 60 Hz on heat-induced expression of heat shock protein 70 (hsp-70) was examined in HL60RG cells. No increase in hsp-70 production was observed in the cells after exposure to 50 mT ELFMF alone. Simultaneous exposure to 50 mT ELFMF in combination with mild heat at 42 and 40 degrees C suppressed heat-induced hsp-70 expression. The suppression of hsp-70 occurred when cells were simultaneously exposed to both for longer periods of more than 5 h. However, the suppression of hsp-70 was not observed at a magnetic density of 5 and 0.5 mT. This result suggests that exposure to 50 mT ELFMF may act on a protection against the concomitant mild heat stress in HL60RG cells.
Miyakoshi
J
J
Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Japan. miyakosh@mfour.med.kyoto-u.ac.jp
Mori
Y
Y
Yaguchi
H
H
Ding
G
G
Fujimori
A
A
D015153:Blotting, Western; D002460:Cell Line; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D008280:Magnetics; D014407:Tumor Cells, Cultured
D018840:HSP70 Heat-Shock Proteins