Isolation of shiga toxin-producing Escherichia coli (STEC) from foods using EHEC agar.
Enterohaemorrhagic Escherichia coli (EHEC) agar was evaluated for its ability to recover one isolate of each of three serotypes (O157:H7, O26 and O113:H21) of shiga toxin-producing E. coli (STEC) from raw mince, pasteurized milk and salami after enrichment. The method detected around one colony-forming unit (cfu) in 25 ml in milk, but was less sensitive with salami, requiring 10-1000 cfu 25 g-1 (depending on serotype) for detection. In raw minced beef any enterohaemolysin-producing colonies were outnumbered by other colonies and only one of 12 enrichments yielded the inoculum serotype. Additional tests were conducted on 15 retail meat products. One 25-g sample of each product was processed as purchased, while another was inoculated with 157-185 cfu of a cocktail of E. coli O157, O113 and O26 cultures. Recovery was easily achieved with cooked meat products and salami. Recovery from raw minced meat was again difficult, but sometimes possible. Testing more suspect colonies than were tested in this study would presumably increase the sensitivity of the method.
Hudson
J A
JA
ESR Ltd, Christchurch Science Centre, Christchurch and ESR Ltd, Kenepuru Science Centre, Porirua, New Zealand. andrew.hudson@esr.cri.nz
Nicol
C
C
Capill
J
J
Bennett
J
J
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D002417:Cattle; D015169:Colony Count, Microbial; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D019453:Escherichia coli O157 / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D005516:Food Microbiology; D008461:Meat Products / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D012703:Serotyping; D022603:Shiga Toxins
D001427:Bacterial Toxins; D003470:Culture Media; D022603:Shiga Toxins