Diagnosis of sporotrichosis in a donkey using direct fluorescein-labeled antibody testing.
A 4-year-old female donkey residing in an open field in Indiana was admitted for evaluation of facial lesions of 2 years duration. Cytologic and histologic examination of exudate and tissue from the lesions revealed a pyogranulomatous inflammatory reaction with numerous yeasts. Sporothrix schenckii was suspected to be the infectious agent; however, multiple culture attempts did not provide positive identification of the organism. Serologic examination supported infection with S. schenckii. A specific direct immunofluorescent antibody test performed on paraffin-embedded tissue sections confirmed the organism as S. schenckii. Clinical signs resolved after appropriate iodide therapy.
Irizarry-Rovira
A R
AR
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907, USA.
Kaufman
L
L
Christian
J A
JA
Reberg
S R
SR
Adams
S B
SB
DeNicola
D B
DB
Rivers
W
W
Hawkins
J F
JF
D000818:Animals; D003937:Diagnosis, Differential; D018397:Equidae / Q000382:microbiology*; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D013175:Sporothrix / Q000276:immunology; D013174:Sporotrichosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*