A cell adhesion protein from the crayfish Pacifastacus leniusculus, a serine proteinase homologue similar to Drosophila masquerade.
A cDNA encoding a protein resembling masquerade, a serine proteinase homologue expressed during embryogenesis, larval, and pupal development in Drosophila melanogaster, was identified in hemocytes of the adult freshwater crayfish, Pacifastacus leniusculus. The crayfish protein is similar to Drosophila masquerade in the following aspects: (a) overall sequence of the serine proteinase domain, such as the position of three putative disulfide bridges, glycine in the place of the catalytic serine residue, and the presence of a substrate-lining pocket typical for trypsins; (b) the presence of several copies of a disulfide-knotted motif in the putative propeptide. This masquerade-like protein is cleaved into a 27-kDa fragment, which could be detected by immunoblot analysis using an affinity-purified antibody against a synthetic peptide in the C-terminal domain of the protein. The 27-kDa protein could be immunoaffinity-purified from hemocyte lysate supernatant and exhibited cell adhesion activity in vitro, indicating that the C-terminal domain of the crayfish masquerade-like protein mediates cell adhesion.
Huang
T S
TS
Department of Comparative Physiology, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18A, SE-752 36 Uppsala, Sweden.
Wang
H
H
Lee
S Y
SY
Johansson
M W
MW
Söderhäll
K
K
Cerenius
L
L
D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000235:genetics* / Q000254:growth & development; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006434:Hemocytes / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D007814:Larva; D008969:Molecular Sequence Data; D011679:Pupa; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D015815:Cell Adhesion Molecules; D029721:Drosophila Proteins; D019476:Insect Proteins; D011994:Recombinant Proteins; C091914:mas protein, Drosophila; D012697:Serine Endopeptidases; C407342:masquerade-like protein, Pacifastacus leniusculus