Pressurization facilitates adenovirus-mediated gene transfer into vein graft.
We investigated whether application of non-distending hydrostatic pressure facilitates gene transfer into vein grafts. An external jugular vein was placed in a chamber with 100 microl adenovirus solution at a titer of 10(10) pfu/ml and was pressurized to up to 8 atm above ambient pressure for 10 min. Histochemical analysis demonstrated a positive transgene expression in all layers of the vessel wall. Gene transfer with 8 atm pressurization resulted in an approximately 50 times higher transgene expression than that without pressurization. Under 8 atm pressurization, the efficiency of gene transfer reached a plateau at 7.5 min. The application of hydrostatic pressure may improve the effectiveness of intraoperative genetic engineering of vein grafts.
Suzuki
M
M
Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo, Japan.
Ishizaka
N
N
Tsukamoto
K
K
Minami
K
K
Taguchi
J
J
Nagai
R
R
Ohno
M
M
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051547:Heme Oxygenase-1; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D007601:Jugular Veins / Q000166:cytology / Q000378:metabolism* / Q000601:surgery / Q000637:transplantation*; D007763:Lac Operon / Q000235:genetics; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012996:Solutions; D019076:Transgenes / Q000235:genetics*; D014182:Transplantation, Autologous
D000911:Antibodies, Monoclonal; D012996:Solutions; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1