title: Flow cytometric detection and analysis of tailless sperm caused by sonication or a chemical agent.
abstract: Flow cytometric analysis has been developed to detect tailless sperm with heads detached from the tails at the neck position. When isolated tailless sperm suspension was subjected to flow cytometry, a second sperm population appeared alongside the normal sperm population on light scatter-histogram. The percentage of this second sperm population (85.2%) was in good agreement with that for the tailless sperm (88.7%) determined microscopically, indicating that the second sperm population would correspond to tailless sperm population in the light scatter-histogram. Rates for tailless sperm determined by flow cytometry significantly correlated with those estimated microscopically following exposure of sperm to either sonication (r = 0.94, P < 0.01), or nitrobenzene (r = 0.80, P < 0.01). The results indicated the utility of the light scatter-histogram in flow cytometry as a simple and convenient procedure for the detection of tailless sperm induced by chemical compounds.
authors.affiliation: Drug Development Laboratories, Yoshitomi Pharmaceutical Industries, Ltd., Hyogo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Yoneyama
authors.forename: M
authors.initials: M
authors.lastname: Imanishi
authors.forename: M
authors.initials: M
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D008027:Light; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation; D013010:Sonication; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects