Fluorescence spectroscopy of normal, SV40-transformed human keratinocytes, and carcinoma cells.
Native fluorescence emission and excitation spectra of SV40 infected human keratinocytes, A431 and SCC324 carcinoma cells, and normal human keratinocytes were measured and compared. A difference in the intracellular metabolic state of NADH was found between the normal cells and the cancer or virus-transformed cells. The observed difference, namely an increased proportion of bound, mitochondrial NADH in the cancer and virus-infected cells, manifests as a blue spectral shift in the emission spectra.
Papadopoulos
A J
AJ
Department of Physics, The City College of the City University of New York, NY 10031, USA.
Zhadin
N N
NN
Steinberg
M L
ML
Alfano
R R
RR
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002461:Cell Line, Transformed / Q000378:metabolism; D002472:Cell Transformation, Viral; D002478:Cells, Cultured / Q000378:metabolism; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D013539:Simian virus 40 / Q000502:physiology; D012878:Skin Neoplasms / Q000473:pathology*; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000378:metabolism
D009243:NAD