title: Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A.
abstract: Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P2(1), with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 A, beta = 101. 33 degrees. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V(m)) of 2.27 A(3) Da(-1) and a solvent content of 46%. Native X-ray data to 2.15 A resolution have been collected using synchrotron X-rays.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151--742, South Korea.
authors.forename: K
authors.initials: K
authors.lastname: Min
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: H K
authors.initials: HK
authors.lastname: Song
authors.forename: C
authors.initials: C
authors.lastname: Chang
authors.forename: S J
authors.initials: SJ
authors.lastname: Cho
authors.forename: J
authors.initials: J
authors.lastname: Moon
authors.forename: J K
authors.initials: JK
authors.lastname: Yang
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: K J
authors.initials: KJ
authors.lastname: Lee
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D046911:Macromolecular Substances; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D046911:Macromolecular Substances; D011994:Recombinant Proteins; D009701:Nucleoside-Diphosphate Kinase