[Activities of trypsin-like serine proteases and their inhibitors in blood and subretinal fluid of patients with different stages of proliferative vitreoretinopathy in rhegmatogenous retinal detachment].
Concentrations of protein, trypsin-like and antitryptic activities, and the level of alpha 2-macroglobulin were measured in the subretinal fluid (SRF) and blood of patients with different stages of proliferative vitreoretinopathy (PVR). The content of protein in SRF of patients with retinal detachment with PVR was by an order of magnitude higher than in the vitreous body and increased from stage B to stage D1, which indicates increased permeability of the blood-eye barrier. Trypsin-like proteolytic enzymes and their main inhibitors are present in the SRF, and changes in their activity indicate augmentation of the inflammatory component of PVR from stage B to stage C3 and transition to a process similar to tissue cicatrization during stage D1. Changes in the proteinase-inhibitor balance in the peripheral blood in PVR are characteristic of a sluggish inflammatory process which activates from stage B to stage C and eventuates in stage D1. Increase in the blood level of alpha 2-macroglobulin during stages C3 and D1 seems to indicate the development of total autoimmune reaction. Antiinflammatory steroid therapy before surgery had a favorable impact on the course of the postoperative period.
Katsnel'son
L A
LA
Chesnokova
N B
NB
Antelava
N D
ND
D015415:Biomarkers; D001813:Blood-Retinal Barrier / Q000502:physiology; D005122:Exudates and Transudates / Q000378:metabolism*; D006801:Humans; D012163:Retinal Detachment / Q000150:complications / Q000201:enzymology* / Q000473:pathology; D012720:Severity of Illness Index; D014357:Trypsin / Q000097:blood / Q000378:metabolism*; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000201:enzymology* / Q000473:pathology; D000515:alpha 1-Antitrypsin / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism*
D015415:Biomarkers; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D014357:Trypsin