title: Analysis of measles virus binding sites of the CD46 gene in patients with subacute sclerosing panencephalitis.
abstract: Measles virus (MV) binding sites of the CD46 gene were sequenced in patients with subacute sclerosing panencephalitis (SSPE) and in controls. There were 3 novel polymorphisms, including C/T at nucleotide position 38 (C/T38), G/A at position 176 (G/A176), and C/T at position 453 (C/T453), at allele frequencies of.97:.03, .99:.01, and.97:.03, respectively. The G/A176 polymorphism causes an Arg/Gln amino acid change within the essential binding sites of MV, whereas the C/A38 polymorphism causes a Ser/Phe change outside the MV binding sites. The C/T453 polymorphism does not produce an amino acid change. Two of the 40 SSPE patients and 2 of the 32 controls had both C/T38 and C/T453 polymorphisms in heterozygous patterns. One control subject, but no SSPE patients, had the G/A176 polymorphism in a heterozygous pattern. Thus, it is not likely that CD46 gene alteration has a role as a host susceptibility factor in the development of SSPE.
authors.affiliation: Dept. of Pediatrics, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. kkusuhar@pediatr.med.kyushu-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Kusuhara
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.forename: F
authors.initials: F
authors.lastname: Nakao
authors.forename: K
authors.initials: K
authors.lastname: Ihara
authors.forename: H
authors.initials: H
authors.lastname: Hattori
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: K
authors.initials: K
authors.lastname: Nihei
authors.forename: N
authors.initials: N
authors.lastname: Koide
authors.forename: H
authors.initials: H
authors.lastname: Aiba
authors.forename: K
authors.initials: K
authors.lastname: Takeshita
authors.forename: T
authors.initials: T
authors.lastname: Hara
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000235:genetics*; D001665:Binding Sites; D002648:Child; D002675:Child, Preschool; D005091:Exons; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008459:Measles virus / Q000378:metabolism*; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D013344:Subacute Sclerosing Panencephalitis / Q000235:genetics* / Q000821:virology
chemical_list: D015703:Antigens, CD; C497857:CD46 protein, human; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins