[PCR detection of Chlamydia pnumoniae DNA in nasopharyngolaryngeal swab samples from patients with rhinitis and pharyngolaryngitis].
A PCR assay was used to detect Chlamydia pneumoniae specific Pst I 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and nasosinusitis. C. pneumoniae specific antibodies in sera were also assayed with microimmuno-fluoresence (MIF). About 28% of the patients (49/175) were PCR positive and 25.7% (45/175) were MIF antibodies positive. The accordance rate of the two methods was 91.8%. It is suggested that the C. pneumoniae Pst I 474 specific PCR is sensitive and specific for detecting C. pneumoniae in pharyngolaryngitis or rhinitis and nasosinusitis.
Zhang
G
G
Third Clinical College, Sun Yat-sen University of Medical Sciences, Guangzhou.
Ning
B
B
D002690:Chlamydia Infections; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D007827:Laryngitis / Q000382:microbiology; D010612:Pharyngitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012220:Rhinitis / Q000382:microbiology; D012852:Sinusitis / Q000382:microbiology
D004269:DNA, Bacterial