A novel bacterium that utilizes monochlorobiphenyls and 4-chlorobenzoate as growth substrates.
A bacterial isolate, tentatively named SK-3, was isolated from tertiary lagoon sludge contaminated with polychlorinated biphenyls for over 25 years. SK-3 was able to grow on 2-chlorobiphenyl, 3-chlorobiphenyl, 4-chlorobiphenyl (4-CB), chloroacetate and 4-chlorobenzoic acid (4-CBA) as sole carbon and energy sources. During growth on 4-CB, a stoichiometric amount of chloride ion was produced and growth yields were comparable to those observed during growth on biphenyl. The production of 4-CBA as a metabolite was not observed. Protein yields during growth on 4-CB and biphenyl suggested that the presence of the chlorine did not impede use of carbons on both aromatic rings. Growth on 4-CBA also resulted in nearly stoichiometric production of chloride ion. Benzoate-grown SK-3 was also able to degrade several Aroclor 1242 congeners without the need for a primary substrate of previous growth on biphenyl.
Kim
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School of Public and Environmental Affairs, Indiana University, Room 231, Bloomington, IN, USA.
Picardal
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D001140:Aroclors / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D002723:Chlorobenzoates / Q000378:metabolism*; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology
D001140:Aroclors; D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D002712:Chlorides; D002723:Chlorobenzoates; D003470:Culture Media; D012722:Sewage; C027423:aroclor 1242; C040768:4-chlorobenzoic acid