title: Partial liquid ventilation with perfluorocarbon in acute lung injury: light and transmission electron microscopy studies.
abstract: Liquid ventilation using perfluorocarbon has been shown to improve gas exchange in animal models of acute lung injury as well as in children with acute respiratory distress syndrome. This study was designed to define structural features of lung injury following partial liquid ventilation (PLV) using light and transmission electron microscopy in a rabbit model of acute respiratory distress. Animals were treated with either conventional mechanical ventilation (CMV-gas) (n = 6) or PLV (n = 5) for 4 h after the induction of acute lung injury with saline lavage. Control animals were killed after the lung injury. PLV significantly improved alveolar-arterial oxygen tension and the oxygen index compared with CMV (P < 0.05). Morphometric studies using light microscopy show less alveolar hemorrhage, less edema, and fewer hyaline membranes in the PLV group (P < 0.05). Polymorphonuclear leukocyte sequestration in lung capillaries (11.4 +/- 1.5 versus 19.2 +/- 3 x 10(8)/ml, P < 0.05, PLV versus CMV) and migration into airspaces (3.1 +/- 1.2 versus 4.5 +/- 1.1 x 10(8)/ml, P < 0.05, PLV versus CMV) were lower in the gravity-dependent lung regions. There were fewer alveolar macrophages in the PLV group compared with other groups (P < 0.05). Fluorescence microscopy analysis shows fewer type II alveolar epithelial cells in the CMV group and brighter type II cells in the PLV group. Transmission electron microscopy studies show more alveolar wall damage in the CMV group, with type II cells detached from their basement membrane with fewer surfactant-containing lamellar bodies. We conclude that quantitative histologic analysis shows less lung damage and inflammation when perfluorocarbon is combined with CMV in the management of acute respiratory distress syndrome.
authors.affiliation: University of British Columbia, Pulmonary Research Laboratory, St. Paul's Hospital, and Children's Hospital, Vancouver, British Columbia, Canada.
authors.forename: S F
authors.initials: SF
authors.lastname: van Eeden
authors.forename: M E
authors.initials: ME
authors.lastname: Klut
authors.forename: M A
authors.initials: MA
authors.lastname: Leal
authors.forename: J
authors.initials: J
authors.lastname: Alexander
authors.forename: Z
authors.initials: Z
authors.lastname: Zonis
authors.forename: P
authors.initials: P
authors.lastname: Skippen
mesh_terms: D000208:Acute Disease; D000818:Animals; D005466:Fluorocarbons / Q000008:administration & dosage / Q000627:therapeutic use*; D006470:Hemorrhage / Q000209:etiology / Q000517:prevention & control; D007249:Inflammation; D008168:Lung / Q000187:drug effects / Q000648:ultrastructure*; D055370:Lung Injury; D016676:Macrophages, Alveolar / Q000648:ultrastructure; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism; D011175:Positive-Pressure Respiration; D011650:Pulmonary Alveoli / Q000648:ultrastructure; D011659:Pulmonary Gas Exchange; D011817:Rabbits; D012121:Respiration, Artificial / Q000379:methods*; D012128:Respiratory Distress Syndrome / Q000473:pathology / Q000628:therapy*; D012965:Sodium Chloride / Q000633:toxicity; D013500:Surface Tension
chemical_list: D005466:Fluorocarbons; C119237:perflubronperfluorocytlbromide; D012965:Sodium Chloride; D010100:Oxygen