Chromatin condensation during spermiogenesis in the golden hamster (Mesocricetus aureus): a flow cytometric study.
DNA-staining of hamster testis cell suspensions followed by flow cytometry demonstrated appearance of the first haploid cells at 23 days post partum (dpp) and of condensed chromatin (in elongated spermatids and spermatozoa) at 33-34 dpp. Mature spermatozoa were first observed in the caput epididymis at 36-37 dpp, thus completing the first spermatogenic wave. Testicular cell suspensions from animals from 23 to 38 dpp were stained with acridine orange, and flow cytometer gating was adjusted to include only the haploid cells. Acridine orange intercalated into double-stranded DNA to produce green fluorescence. The decrease in green fluorescence intensity from 23 until 37 dpp was caused by changes in the binding of DNA to basic proteins in such a fashion as to impede the access of the dye to the DNA double helix. When the green fluorescence values (of the most advanced spermatids) were plotted against the age of the hamsters (in dpp) or the corresponding steps of spermiogenesis, the decrease in fluorescence could be seen to occur in three phases. The inflection point between the first and second phases was observed at about spermiogenesis step 7, consistent with the hypothesis that this represents removal of histone from the chromatin. The second phase presumably represents the period in which transition proteins are bound to the DNA. At approximately steps 15 or 16 a further inflection point was seen where protamines replaced the transition proteins. The red fluorescence produced when acridine orange bound to RNA in spermatids, increased early in spermiogenesis and decreased dramatically at 34 dpp, consistent with the fact that elongating spermatids discard the bulk of their cytoplasm during the maturation process.
Golan
R
R
Department of Clinical Biochemistry, Sackler Medical School, Tel Aviv University, Ramat Aviv, Israel. rachelgo@post.tau.ac.il
Vigodner
M
M
Oschry
Y
Y
Shochat
L
L
Lewin
L M
LM
D000818:Animals; D002843:Chromatin / Q000502:physiology*; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D008297:Male; D008647:Mesocricetus; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000378:metabolism
D002843:Chromatin; D004247:DNA