Virulence-related DNA sequences and adherence patterns in strains of enteropathogenic Escherichia coli.
The presence of the genes for Escherichia coli adherence factor (EAF), attaching and effacing lesion (eae) and bundle-forming pili (bfp) in 72 strains identified as enteropathogenic E. coli (EPEC) by slide agglutination was evaluated using hybridization and PCR. The adherence property of these strains was assayed using 3h HeLa cells adherence assay. The results obtained indicated that virulence-associated genes were present in 65% of the strains but only ten (13.9%) isolates were positive for all the three markers (typical EPEC), 37 (51.4%) isolates carried either one or two of these determinants (atypical EPEC) and the remaining 25 (34.7%) were negative for all these genes. In vitro adherence assay showed that 44 (61.1%) strains adhered to HeLa cells with a defined pattern, 13 (18.1%) isolates adhered loosely with no definite pattern and the remaining 15 (20.8%) were non-adherent. Analysis of the results showed a statistically significant association between the presence of the virulence-related genes with adherence of the strains with a defined pattern (P</=0.0001). These results indicated that since over 60% of the strains identified by serogrouping carried at least one of the putative virulence markers, it therefore seems that this simple test is still of value in our setting although the need for a confirmatory test is also indicated.
Bouzari
S
S
Molecular Biology Unit, Pasteur Institute of Iran, Pasteur Ave., Tehran, Iran. bouzari@institute.pasteur.ac.ir
Jafari
M N
MN
Shokouhi
F
F
Parsi
M
M
Jafari
A
A
D001422:Bacterial Adhesion; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial