Parasympathetic non-adrenergic, non-cholinergic-induced protein synthesis and mitogenic activity in rat parotid glands.
Electrical stimulation of the parasympathetic auriculo-temporal nerve (40 Hz, 30 min), in the anaesthetized rat under - and -adrenoceptor blockade, increased [3H]thymidine and [3H]leucine uptake into the parotid glands by 80 and 263 %, respectively. The increase in response to parasympathetic stimulation was almost the same ([3H]thymidine 82 % and [3H]leucine 283 %) when atropine (2 mg kg-1 I.P. or I.V.) was included in the pretreatment. Neither intravenous infusion of vasoactive intestinal peptide (0.5-20 mg kg-1 min-1, 30 min) nor of bethanechol (10 mg kg-1 min-1, 30 min), under adrenoceptor blockade, increased the uptake of [3H]thymidine into the glands. However, these drugs increased [3H]leucine uptake, and in combination they interacted positively. Whereas vasoactive intestinal peptide is likely to be involved in the parasympathetic nerve-evoked protein synthesis, the nature of the non-adrenergic, non-cholinergic component(s) involved in the mitogenic response is presently unknown.
Ekström
J
J
Department of Pharmacology, Institute of Physiology and Pharmacology, Goteborg University, Box 431, 405 30 Göteborg, Sweden.
Havel
G E
GE
Reinhold
A
A
D018674:Adrenergic Antagonists / Q000494:pharmacology; D000818:Animals; D018723:Bethanechol / Q000494:pharmacology; D018680:Cholinergic Antagonists / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010275:Parasympathetic Nervous System / Q000502:physiology*; D010277:Parasympathomimetics / Q000494:pharmacology; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects / Q000294:innervation* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D018674:Adrenergic Antagonists; D018680:Cholinergic Antagonists; D009419:Nerve Tissue Proteins; D010277:Parasympathomimetics; D018723:Bethanechol; D014660:Vasoactive Intestinal Peptide