Adhesive strength of autologous fibrin glue.
To establish an easy and rapid method for measuring the adhesive strength of fibrin glue and to clarify the factor(s) most affecting the strength, a study was made on the effect of the concentration of plasma components on the strength of cryoprecipitate (Cryo) prepared from a subject's own autologous plasma to be used as fibrin glue. The adhesive strength of the Cryo was measured with various supporting materials instead of animal skin using a tester of tension and compression. The results were as follows: (1) the strength of Cryo applied to ground flat glass (4 cm2) was significantly greater than that applied to clear glass, clear plastic, or smooth and flat wood chips; (2) the adhesive strength of Cryo depended on the concentration of thrombin with the optimal concentration being 50 units/ml; (3) the concentration of CaCl2 did not affect the adhesive strength of Cryo; (4) the adhesive reaction was dependent on the temperature and the adhesive strength more quickly reached a steady state at 37 degrees C than at lower temperature; (5) the adhesive strength was correlated well with the total concentration of fibrinogen and fibronectin. These results indicate that the adhesive strength of Cryo can be easily and quickly evaluated using a tester and ground glass with thrombin at 50 units/ml, and that the adhesive strength of Cryo can be predicted from the total concentration of fibrinogen and fibronectin.
Yoshida
H
H
Department of Pharmacy, Yamaguchi University Hospital, Ube, Japan.
Hirozane
K
K
Kamiya
A
A
D002122:Calcium Chloride / Q000032:analysis; D005337:Fibrin; D006801:Humans; D008422:Materials Testing; D015203:Reproducibility of Results; D013696:Temperature; D013917:Thrombin / Q000032:analysis; D014014:Tissue Adhesives / Q000737:chemistry
D014014:Tissue Adhesives; D005337:Fibrin; D013917:Thrombin; D002122:Calcium Chloride