title: Effect of prostaglandin E2 on cytosolic calcium ion and morphology in cultured bovine retinal pigment epithelium.
abstract: Inflammatory conditions of the eye may cause migration and proliferation of retinal pigment epithelium (RPE) cells. Therefore we evaluated the effects of prostaglandin E2 (PGE2) on cytosolic calcium ion concentration ([Ca2+]i) and on morphological changes in cultured bovine RPE cells. PGE2 caused an increase in [Ca2+]i, and contraction of RPE cells noted on phase-contrast biomicroscopy. The increase of [Ca2+]i was restrained by removing calcium ion from the medium and the addition of verapamil. Contraction of RPE cells was evaluated further by measuring the diffusion of 3H-dextran. The PGE2-induced increase in diffusion of 3H-dextran was suppressed by verapamil. We conclude that the contraction of RPE cells induced by PGE2 is dependent upon calcium ion transient and may be countered by calcium-blocking agents.
authors.affiliation: Department of Ophthalmology, Fukushima Medical University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sekiryu
authors.forename: T
authors.initials: T
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D003911:Dextrans / Q000378:metabolism; D004058:Diffusion; D015232:Dinoprostone / Q000494:pharmacology*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D003911:Dextrans; D015232:Dinoprostone; D002118:Calcium