title: Enantioselective analysis of methadone in saliva by liquid chromatography-mass spectrometry.
abstract: Saliva was tested and evaluated as a biological matrix for methadone (Mtd) monitoring. Conventional method using a narrow bore C18 column, and an enantioselective method using a narrow bore alpha1-acid glycoprotein column, were developed using liquid chromatography coupled with a mass spectromeric (MS) detector. After optimisation of MS conditions by flow injection analysis, selected ion monitoring detection was used to enhance sensitivity. The total Mtd concentration and the enantiomeric ratio in saliva were validated using an experimental design. The methods were applied to samples provided by heroin addicts undergoing a Mtd treatment. Results on total Mtd determination showed a very poor correlation between saliva and serum, whereas the enantiomeric ratios of Mtd gave a very good one.
authors.affiliation: Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Ortelli
authors.forename: S
authors.initials: S
authors.lastname: Rudaz
authors.forename: A F
authors.initials: AF
authors.lastname: Chevalley
authors.forename: A
authors.initials: A
authors.lastname: Mino
authors.forename: J J
authors.initials: JJ
authors.lastname: Deglon
authors.forename: L
authors.initials: L
authors.lastname: Balant
authors.forename: J L
authors.initials: JL
authors.lastname: Veuthey
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry; D008691:Methadone / Q000032:analysis* / Q000652:urine; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008691:Methadone