Novel mechanism that Trypanosoma cruzi uses to adhere to the extracellular matrix mediated by human galectin-3.
Binding of Trypanosoma cruzi trypomastigotes to laminin is enhanced by galectin-3, a beta-galactoside binding lectin. The galectin-3 enhanced binding of trypanosomes to laminin is inhibited by lactose. Co-immunoprecipitations indicate that galectin-3 binds to the 45, 32 and 30 kDa trypanosome surface proteins. Binding of galectin-3 to the 45, 32 and 30 kDa surface proteins is inhibited by lactose. Polyclonal and a monoclonal antibodies to galectin-3 immunoprecipitated a major 64 kDa trypanosome surface protein. T. cruzi monoclonal antibody to mucin recognized the 45 kDa surface protein. The 45, 32 and 30 kDa surface proteins interact with galectin-3 in order to enhance trypanosome adhesion to laminin.
Moody
T N
TN
Department of Microbiology, School of Medicine, Meharry Medical College, 1005 Dr. D.B. Todd Jr. Blvd., Nashville, TN 37208, USA.
Ochieng
J
J
Villalta
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D003429:Cross Reactions / Q000276:immunology; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism* / Q000469:parasitology; D037502:Galectin 3; D006801:Humans; D007785:Lactose / Q000378:metabolism / Q000494:pharmacology; D007797:Laminin / Q000378:metabolism*; D008970:Molecular Weight; D009077:Mucins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding / Q000187:drug effects; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D037502:Galectin 3; D007797:Laminin; D009077:Mucins; D014643:Variant Surface Glycoproteins, Trypanosoma; D003094:Collagen; D007785:Lactose