Effect of copper speciation on whole-cell soluble methane monooxygenase activity in Methylosinus trichosporium OB3b.
Soluble methane monooxygenase (sMMO) activity in Methylosinus trichosporium OB3b was found to be more strongly affected as copper-to-biomass ratios changed in a newly developed medium, M2M, which uses pyrophosphate for metal chelation, than in nitrate mineral salts (NMS), which uses EDTA. When M2M medium was amended with EDTA, sMMO activity was similar to that in NMS medium, indicating that EDTA-bound copper had lower bioavailability than pyrophosphate-bound copper. EDTA did not limit the association of copper with the cells; rather, copper was sequestered in a form which did not affect sMMO activity.
Morton
J D
JD
Environmental and Water Resources Engineering, Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan 48109-2125, USA.
Hayes
K F
KF
Semrau
J D
JD
D002021:Buffers; D002614:Chelating Agents; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D003470:Culture Media; D011756:Diphosphates; D004492:Edetic Acid; D020602:Methylosinus trichosporium / Q000201:enzymology*; D010105:Oxygenases / Q000378:metabolism*
D002021:Buffers; D002614:Chelating Agents; D003470:Culture Media; D011756:Diphosphates; D003300:Copper; D004492:Edetic Acid; D010105:Oxygenases; C021555:methane monooxygenase