Strong immunoreactivity of platelet-derived growth factor and its receptor at human and mouse neuromuscular junctions.
Platelet-derived growth factor (PDGF) and its alpha-receptor were localized at human and mouse neuromuscular junctions (NMJs) using specific polyclonal antibodies against each, anti-PDGF-A and anti-PDGF alpha-receptor, respectively. By applying double fluorescence labeling, immunoreactivity for PDGF and its receptor was closely co-localized with acetylcholine receptors, which were identified with alpha-bungarotoxin. PDGF might be involved in the interaction between the presynaptic and postsynaptic components. This is the first demonstration of PDGF and its receptor concentrated at human and mouse NMJs.
Zhao
Y
Y
Department of Pediatrics, Tohoku University School of Medicine, Sendai, Japan.
Haginoya
K
K
Iinuma
K
K
D000818:Animals; D002038:Bungarotoxins; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000166:cytology / Q000294:innervation; D009469:Neuromuscular Junction / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000032:analysis*; D011950:Receptors, Cholinergic / Q000032:analysis
D002038:Bungarotoxins; D010982:Platelet-Derived Growth Factor; D011950:Receptors, Cholinergic; D020796:Receptor, Platelet-Derived Growth Factor alpha