title: Characterization of proanthocyanidins from grape seeds.
abstract: Leucoselect (grape seed selected proanthocyanidins) was analyzed. HPLC thermospray mass spectrometry (TSP-MS) allowed the detection of monomeric flavan-3-ols and dimeric proanthocyanidins. Fractionation over Sephadex LH-20 resin and analysis of the isolated fractions by gel permeation chromatography (GPC) and electrospray mass spectrometry (ESI-MS) led to the complete characterization of the proanthocyanidin constituents of Leucoselect. The analysis revealed the presence of approximately 15% of (+)-catechin (1) and (-)-epicatechin (2), 80% of (-)-epicatechin 3-O-gallate (3), dimers, trimers, tetramers and their gallates and 5% of pentamers, hexamers, heptamers and their gallates.
authors.affiliation: Indena SpA, Laboratori Ricerca e Sviluppo, Via Don Minzoni 6, 20090, Settala, Milano, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Gabetta
authors.forename: N
authors.initials: N
authors.lastname: Fuzzati
authors.forename: A
authors.initials: A
authors.lastname: Griffini
authors.forename: E
authors.initials: E
authors.lastname: Lolla
authors.forename: R
authors.initials: R
authors.lastname: Pace
authors.forename: T
authors.initials: T
authors.lastname: Ruffilli
authors.forename: F
authors.initials: F
authors.lastname: Peterlongo
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry; D044945:Proanthocyanidins; D019685:Rosales; D012639:Seeds
chemical_list: D000872:Anthocyanins; D044945:Proanthocyanidins; C013221:proanthocyanidin