Uptake of lipoprotein-associated alpha-tocopherol by primary porcine brain capillary endothelial cells.
From the severe neurological syndromes resulting from vitamin E deficiency, it is evident that an adequate supply of the brain with alpha-tocopherol (alphaTocH), the biologically most active member of the vitamin E family, is of utmost importance. However, uptake mechanisms of alphaTocH in cells constituting the blood-brain barrier are obscure. Therefore, we studied the interaction of low (LDL) and high (HDL) density lipoproteins (the major carriers of alphaTocH in the circulation) with monolayers of primary porcine brain capillary endothelial cells (pBCECs) and compared the ability of these two lipoprotein classes to transfer lipoprotein-associated alphaTocH to pBCECs. With regard to potential binding proteins, we could identify the presence of the LDL receptor and a putative HDL3 binding protein with an apparent molecular mass of 100 kDa. At 4 degrees C, pBCECs bound LDL with high affinity (K(D) = 6 nM) and apolipoprotein E-free HDL3 with low affinity (98 nM). The binding capacity was 20,000 (LDL) and 200,000 (HDL3) lipoprotein particles per cell. alphaTocH uptake was approximately threefold higher from HDL3 than from LDL when [14C]alphaTocH-labeled lipoprotein preparations were used. The majority of HDL3-associated alphaTocH was taken up in a lipoprotein particle-independent manner, exceeding HDL3 holoparticle uptake 8- to 20-fold. This uptake route is less important for LDL-associated alphaTocH (alphaTocH uptake approximately 1.5-fold higher than holoparticle uptake). In line with tracer experiments, mass transfer studies with unlabeled lipoproteins revealed that alphaTocH uptake from HDL3 was almost fivefold more efficient than from LDL. Biodiscrimination studies indicated that uptake efficacy for the eight different stereoisomers of synthetic alphaTocH is nearly identical. Our findings indicate that HDL could play a major role in supplying the central nervous system with alphaTocH in vivo.
Goti
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Institute of Medical Biochemistry, University of Graza, Austria.
Hammer
A
A
Galla
H J
HJ
Malle
E
E
Sattler
W
W
D000818:Animals; D000975:Antioxidants / Q000493:pharmacokinetics; D001812:Blood-Brain Barrier / Q000502:physiology*; D001921:Brain / Q000098:blood supply* / Q000166:cytology; D001923:Brain Chemistry / Q000502:physiology; D002196:Capillaries / Q000378:metabolism; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D008078:Cholesterol, LDL / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D007536:Isomerism; D008075:Lipoproteins, HDL / Q000378:metabolism / Q000493:pharmacokinetics*; D053440:Lipoproteins, HDL3; D018110:Receptors, Lipoprotein / Q000378:metabolism; D013552:Swine; D014810:Vitamin E / Q000737:chemistry / Q000493:pharmacokinetics*
D000975:Antioxidants; D002250:Carbon Radioisotopes; D008078:Cholesterol, LDL; D008075:Lipoproteins, HDL; D053440:Lipoproteins, HDL3; D018110:Receptors, Lipoprotein; D014810:Vitamin E