[Generation of Kruppel phenocopies by injecting into Drosophila embryos RNA complementary to mRNA in parallel orientation].
RNA preparations synthesized in vitro were used to study the influence of RNA interference on the Kruppel gene activity in Drosophila embryos. RNA complementary in parallel orientation to the mRNA fragment proved to induce the development of Kruppel phenocopies. The data obtained indicate that mechanisms of specific regulation of gene activity exist in Drosophila cells, which are sensitive to the formation of both parallel and antiparallel RNA-RNA duplexes that include mRNA of the corresponding gene.
Churikov
N A
NA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. tchur@imb.ac.ru
Shostak
N G
NG
Okladnova
O V
OV
Chernov
B K
BK
D000818:Animals; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005801:Genes, Homeobox; D008845:Microinjections; D010641:Phenotype; D018075:RNA, Complementary / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D018075:RNA, Complementary; D012333:RNA, Messenger