Comparison of three methods of acute administration of progesterone on ovarian follicular development and the timing and synchrony of ovulation in Bos indicus heifers.
The aim of this study was to induce the formation of a persistent dominant ovarian follicle and to compare the effects of 3 methods of acute administration of P4 on ovarian follicular development and on the timing and synchrony of ovulation. Stage of the estrous cycle was initially synchronized in Bos indicus heifers with a norgestomet implants (3 mg) for 10 d and with an analogue of PGF2 alpha (15 mg) on the first and last day of norgestomet treatment. Eight days after removal of the implants, heifers were randomly assigned to 4 groups. All heifers received a norgestomet implant (Day 0), which was removed 17 d later (Day 17); PGF2 alpha was administered on Days 0 and 4. Heifers in the control group (n = 5) received no other treatment. On Day 10 heifers in Group P4C (n = 5) were treated with a CIDR for 24 h; heifers in Group P4O (n = 5) were administered 100 mg i.m. of P4 in oil, while heifers in Group P4S (n = 5) were administered 100 mg i.m. of P4 in saline/alcohol. Data were analyzed using bootstrap estimates of location (mean) and spread (standard deviation; SD). Compared with the control heifers, day of emergence of the ovulatory follicle was delayed, and age and duration of dominance of the ovulatory follicle were reduced in the P4C and P4O heifers (P < 0.05) but not in the P4S heifers (P > 0.05). In all groups treated with P4 both the mean and variability (SD) in the timing of ovulation did not differ with that of the control group (P > 0.05) but there was less variability in the day of emergence, age, duration of dominance and diameter of the ovulatory follicle than in the control group (P < 0.05). Delayed timing and reduced synchrony (SD) of ovulation and greater age of the ovulatory follicle (P < 0.05) occurred in P4S heifers than in P4C heifers. We conclude that administration of 100 mg of P4 in oil is as effective as treatment with a CIDR for synchronizing emergence and ovulation of a newly recruited dominant follicle. However, reduced synchrony of ovulation, greater age of the ovulatory follicle and delayed timing of ovulation occurred following administration 100 mg of P4 in saline/alcohol compared with the CIDR device.
Cavalieri
J
J
Australian Institute of Tropical Veterinary & Animal Science, James Cook University, Townsville, Australia.
Coleman
C
C
Kinder
J E
JE
Fitzpatrick
L A
LA
D000282:Administration, Intravaginal; D000818:Animals; D002417:Cattle / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D004973:Estrus Synchronization / Q000187:drug effects / Q000502:physiology*; D005260:Female; D007273:Injections, Intramuscular / Q000662:veterinary; D015999:Multivariate Analysis; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D010053:Ovary / Q000000981:diagnostic imaging; D011282:Pregnenediones / Q000627:therapeutic use; D011374:Progesterone / Q000008:administration & dosage* / Q000097:blood / Q000502:physiology; D045167:Progesterone Congeners / Q000627:therapeutic use; D011461:Prostaglandins F, Synthetic / Q000627:therapeutic use; D011863:Radioimmunoassay / Q000662:veterinary; D011897:Random Allocation; D012044:Regression Analysis; D014463:Ultrasonography
D011282:Pregnenediones; D045167:Progesterone Congeners; D011461:Prostaglandins F, Synthetic; C100284:norgestomet; D011374:Progesterone; D004958:Estradiol; C049643:luprostenol