On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis--fluorescence line narrowing spectroscopy.
The benzo[a]pyrene (BP)-derived 7-(benzo[a]pyren-6-yl)guanine (BP-6-N7Gua) depurinating one-electron oxidation adduct was identified in the urine extracts of coal-smoke-exposed humans for the first time. Urine samples were prepared by solid-phase extraction and reversed-phase high-performance liquid chromatography. Subsequently, the BP-6-N7Gua adduct was identified on-line with capillary electrophoresis-- fluorescence line narrowing spectroscopy (CE-FLNS) at 4.2 K. The daily excretion of BP-6-N7Gua in human urine of individuals exposed to coal smoke was approximately 226 pmol per micromol of creatinine. Due to the high level of excretion we propose that BP-6-N7Gua adducts found in urine could serve as effective biomarkers for risk assessment of BP exposure. The results demonstrate that CE-FLNS allows for on-line separation and DNA adducts identification in complex fluid extracts.
Roberts
K P
KP
USDOE and Department of Chemistry, Iowa State University, Ames 50011, USA.
Lin
C H
CH
Singhal
M
M
Casale
G P
GP
Small
G J
GJ
Jankowiak
R
R
D001580:Benzopyrenes / Q000032:analysis*; D003031:Coal; D003404:Creatinine / Q000097:blood; D018736:DNA Adducts / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D004781:Environmental Exposure; D004867:Equipment Design; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D009862:Online Systems; D012680:Sensitivity and Specificity; D012906:Smoke; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
C102824:7-(benzo(a)pyren-6-yl)guanine; D001580:Benzopyrenes; D003031:Coal; D018736:DNA Adducts; D012906:Smoke; D006147:Guanine; D003404:Creatinine