[Effects of the new compound-XW630 on osteoblast].
This study aimed to observe the influence of the new compound-XW630 on the proliferation of osteoblast, ALP action, and the forming of osteoclast. The MTT method and the alkaline phosphatase method were adopted to investigate the influence of XW630 on ALP action and the proliferation of osteoblast cultured from grown rat's skull. The Tartrate-resistant acid phosphatase dyeing method was used to observe the influence of XW630 on forming of cultured osteoclast in vitro. The result showed that, when XW630 concentration was 10(-6)-10(-8) mol/L, it obviously enhanced the proliferation of osteoblast and improved the activity of ALP, and it evidently provented PTH from stimulating the forming of osteoclast. It is concluded that XW630 is obviously effective for stimulating the proliferation of cultivated osteoblast in vitro and for inhibiting the forming of osteoclast.
Deng
L
L
Department of Pharmacochemistry, School of Pharmacy, Chengdu.
Chen
H
H
Zheng
H
H
Weng
L
L
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010012:Osteogenesis / Q000187:drug effects*; D010024:Osteoporosis / Q000188:drug therapy; D051381:Rats; D017208:Rats, Wistar
D000469:Alkaline Phosphatase