A sensitive LC-MS/MS method for the quantification of fluticasone propionate in human plasma.
A sensitive and selective LC-(APCI) MS/MS method capable of quantifying fluticasone propionate (FP) at levels down to 10 pg ml(-1) in human plasma is reported. The method was validated over a linear range from 10 to 1000 pg ml(-1) using a previously published solid-phase extraction procedure with a 13C3-labeled internal standard. The inter and intra batch precision (coefficient of variation) and accuracy (% bias) of the quality controls samples (20, 25, 50, 100, 200, 500 and 1000 pg ml(-1)) were less than 15 and 11%, respectively. The method is robust, rapid (analysis time of 2 min), selective and hence is ideally suited for pharmacokinetic investigations involving inhalation of therapeutic doses of FP.
Krishnaswami
S
S
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville 32610, USA.
Möllmann
H
H
Derendorf
H
H
Hochhaus
G
G
D000280:Administration, Inhalation; D000730:Androstadienes / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D000068298:Fluticasone; D005615:Freezing; D006801:Humans; D013058:Mass Spectrometry; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
D000730:Androstadienes; D000893:Anti-Inflammatory Agents; D000068298:Fluticasone