title: Hydroxyurea inhibits intracellular Toxoplasma gondii multiplication.
abstract: Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected host cells were treated with hydroxyurea for periods varying from 5 to 48 h, and the survival and morphology of the parasite were determined. Hydroxyurea arrested intracellular T. gondii multiplication in all periods tested. After 48 h of incubation with hydroxyurea, intracellular parasites were not easily observed in Vero cells. Ultrastructural observations showed that infected host cells treated with hydroxyurea for 24 h or more presented disrupted intracellular parasites within the PV. However, the host cells exhibited a normal morphology. Our observations suggest that hydroxyurea was able to interfere with the cycle of the intracellular parasite, leading to the complete destruction of the T. gondii without affecting the host cells.
authors.affiliation: Laboratório de Biologia Celular, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego 2000, Campos dos Goytacazes, CEP 28015-620, Rio de Janeiro, Brazil. ejtm@cbb.uenf.br
authors.forename: E J
authors.initials: EJ
authors.lastname: de Melo
authors.forename: R O
authors.initials: RO
authors.lastname: Mayerhoffer
authors.forename: W
authors.initials: W
authors.lastname: de Souza
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D006790:Host-Parasite Interactions; D006918:Hydroxyurea / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D014122:Toxoplasma / Q000187:drug effects* / Q000254:growth & development; D014709:Vero Cells / Q000469:parasitology / Q000648:ultrastructure
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D006918:Hydroxyurea