title: Release of TNFalpha in response to SiC fibres: differential effects in rodent and human primary macrophages, and in macrophage-like cell lines.
abstract: Asbestos has been implicated in the pathogenesis of several lung diseases, but its mechanism of action is not fully understood. However, asbestos-induced oxidative stress and production of inflammatory cytokines may play a significant role. TNFalpha is an inflammatory cytokine which has a central role in inflammation and fibrosis due to its ability to stimulate fibroblasts and collagen deposition. In this study, a panel of fibres designated either pathogenic or non-pathogenic in recent animal studies, were utilized. The amount of TNFalpha released after a 16-hour exposure to the panel of fibres was compared in four different cell types; two primary macrophage cell types and two cell lines. TNFalpha release by cells exposed to the panel did not equate to pathogenicity, although the most pathogenic fibre caused three out of the four cell types tested, to produce the greatest amount of TNFalpha. Primary rat cells and primary human cells behaved in a similar manner as regards to TNFalpha production; the cell lines behaved quite differently to their primary counterparts with regards to TNFalpha production in this study.
authors.affiliation: Department of Biological Sciences, Napier University, Edinburgh EH10 5DT, UK.
authors.forename: C E
authors.initials: CE
authors.lastname: Fisher
authors.forename: A G
authors.initials: AG
authors.lastname: Rossi
authors.forename: J
authors.initials: J
authors.lastname: Shaw
authors.forename: P H
authors.initials: PH
authors.lastname: Beswick
authors.forename: K
authors.initials: K
authors.lastname: Donaldson
mesh_terms: D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D019016:Mineral Fibers / Q000633:toxicity*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D019016:Mineral Fibers; D014409:Tumor Necrosis Factor-alpha; D001194:Asbestos