Anticlastogenic potential of 1alpha,25-dihydroxyvitamin D3 in murine lymphoma.
Vitamin D3, having gained scientific interest for so long because of its role in mineral homeostasis, has now received great importance as a possible antitumor agent. This study was undertaken in an attempt to visualize the possible anticlastogenic potential of the vitamin in an ascitic mouse lymphoma model namely, Dalton's lymphoma. Frequencies of structural type chromosomal aberrations, sister chromatid exchanges and micronucleus assays have been chosen as the genotoxic endpoints in the proposed investigation. All these cytogenetic markers have been found to be markedly elevated during the progression of lymphoma in bone marrow cells. Vitamin D3 effectively suppressed the frequencies of chromosomal aberrations and sister chromatid exchanges in the lymphoma-bearing mice during the entire phase of tumor growth that significantly coupled with almost two-fold increase in survival time (37 +/- 2 and 68 +/- 2 days in lymphoma controls and vitamin D3-treated lymphoma-bearing mice, respectively), thus substantiating the antineoplastic efficacy of this secosteroid. The outcome of this study also is clearly reflected in the depletion of circulating (serum) vitamin D3 levels in the lymphoma control mice compared with normal (vehicle) controls while a still higher level was maintained in the VD3-treated lymphoma mice. This anticlastogenic property of the vitamin has so far been neglected and this is the first attempt to unravel the vitamin D3's effect in combating tumor development in vivo by limiting the frequencies of chromosomal aberrations, sister chromatid exchanges and micronuclei at least in transplantable murine model studied herein.
Sarkar
A
A
Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Saha
B K
BK
Basak
R
R
Mukhopadhyay
I
I
Karmakar
R
R
Chatterjee
M
M
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002117:Calcitriol / Q000097:blood / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000235:genetics; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000254:growth & development; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D009368:Neoplasm Transplantation; D009929:Organ Size / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects
D016587:Antimutagenic Agents; D002117:Calcitriol