Phage display of RNase A and an improved method for purification of phages displaying RNases.
Functional ribonuclease A was presented on the surface of the filamentous phage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modified method for preparing RNase-displaying phages without contaminating host RNases.
Korn
K
K
Institut für Biochemie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Germany.
Foerster
H H
HH
Hahn
U
U
D000818:Animals; D001435:Bacteriophages / Q000201:enzymology* / Q000302:isolation & purification*; D036022:Capsid Proteins; D002417:Cattle; D004268:DNA-Binding Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000138:chemical synthesis; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins; D012259:Ribonuclease, Pancreatic / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000378:metabolism
D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; D012259:Ribonuclease, Pancreatic