Combined assessment of tau and neuronal thread protein in Alzheimer's disease CSF.
OBJECTIVE
Comparative study of CSF levels of tau and AD7C-neuronal thread protein (NTP) in patients with AD and control subjects.


BACKGROUND
AD is characterized by neurofibrillary tangles composed of the abnormally hyperphosphorylated microtubule-associated protein tau. AD7C-NTP is a proposed AD marker expressed at early stages of neurofibrillary degeneration.


METHODS
Enzyme-linked immunosorbent assays specific for tau and AD7C-NTP. CSF samples were obtained from 35 demented patients (25 with antemortem clinical diagnosis of probable AD, 5 with neuropathologic diagnosis of definite AD, 5 with Lewy body pathology), 29 nondemented patients with PD, and 16 elderly healthy control subjects. Receiver operating characteristics (ROC) and multivariate discriminant analysis for AD versus controls. Correlational analysis of CSF tau and AD7C-NTP and of each marker with Mini-Mental State Examination (MMSE) scores was performed.


RESULTS
Levels of both tau and AD7C-NTP were significantly elevated in the AD patients compared with control subjects. ROC analysis showed that CSF tau distinguished between patients with AD and nondemented control subjects with 63% sensitivity and 89% specificity, AD7C-NTP with 70% sensitivity and 87% specificity. Combined evaluation of both markers with discriminant analysis raised the specificity to 93% at a 63% sensitivity level. Both markers positively correlated with each other within the AD group, but not among control subjects. CSF levels of AD7C-NTP, but not of tau, showed a small but significant inverse correlation (r = -0.43) with MMSE scores of AD patients.


CONCLUSIONS
CSF levels of tau and AD7C-NTP may be useful biomarkers for AD.
Kahle
P J
PJ
Departments of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jakowec
M
M
Teipel
S J
SJ
Hampel
H
H
Petzinger
G M
GM
Di Monte
D A
DA
Silverberg
G D
GD
Möller
H J
HJ
Yesavage
J A
JA
Tinklenberg
J R
JR
Shooter
E M
EM
Murphy
G M
GM
D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D016002:Discriminant Analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009419:Nerve Tissue Proteins / Q000134:cerebrospinal fluid*; D009483:Neuropsychological Tests; D010300:Parkinson Disease / Q000134:cerebrospinal fluid; D011237:Predictive Value of Tests; D012372:ROC Curve; D012680:Sensitivity and Specificity; D016875:tau Proteins / Q000134:cerebrospinal fluid*
C109804:AD7c-NTP; D015415:Biomarkers; D009419:Nerve Tissue Proteins; D016875:tau Proteins