The distribution of aminoacylase I among mammalian species and localization of the enzyme in porcine kidney.
Aminoacylase I (Acy-1, EC 3.5.1.14) is found in many mammalian tissues, with highest activities occurring in kidney. The enzyme hydrolyzes a variety of N-acylated amino acids; however, the physiological role and the exact cellular localization of Acy-1 are still a matter of debate. The comparison of Acy-1 activities in kidney and liver homogenates of 11 mammalian species showed that the enzyme is most abundant in true herbivores such as sheep and cattle as well as in omnivores, while activities were very low in both rodents and the cat. Acy-1 activity was not detected in livers of dogs of five different breeds. Using in situ hybridization of porcine kidney sections with DIG-labeled RNA probes, Acy-1 mRNA was shown to be evenly distributed throughout the tubular system, while glomeruli and the interstitium were free of stain. During subcellular fractionation, porcine Acy-1 behaved like a typical cytosolic enzyme. Commonly, Acy-1 is thought to catalyze hydrolytic reactions, i.e., the formation of free amino acids from acylated derivatives. Based on the present results and literature data, we propose a novel hypothesis, i.e., that Acy-1 catalyzes the synthesis (rather than the hydrolysis) of hippurate that is formed as a detoxification product of aromatic compounds.
Lindner
H
H
Institute of Physiological Chemistry, School of Medicine, Philipps-University, Institute of Physiological Chemistry, Karl von Frisch Strasse 1, 35033, Marburg, Germany.
Höpfner
S
S
Täfler-Naumann
M
M
Miko
M
M
Konrad
L
L
Röhm
K H
KH
D000581:Amidohydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002499:Centrifugation, Density Gradient; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D007668:Kidney / Q000201:enzymology*; D008322:Mammals; D013045:Species Specificity; D013347:Subcellular Fractions; D013552:Swine
D000581:Amidohydrolases; C019672:aminoacylase I