title: Automatic quantification of liver fibrosis: design and validation of a new image analysis method: comparison with semi-quantitative indexes of fibrosis.
abstract: OBJECTIVE
  Liver fibrosis is one of the most important and characteristic histologic alterations in progressive and chronic liver diseases. Thus, in both clinical and experimental practice, it is fundamental to have a reliable and objective method for its precise quantification. Several semi-quantitative scoring systems have been described. All are time-consuming and produce partially subjective fibrosis evaluations that are not very precise. This paper describes the design and validation of an original image analysis-based application, FibroQuant, for automatically and rapidly quantifying perisinusoidal, perivenular and portal-periportal and septal fibrosis and portal-periportal and septal morphology in liver histologic specimens.
  
  
  METHODS
  The implemented image-processing algorithms automatically segment interstitial fibrosis areas, while extraction of portal-periportal and septal region is carried out with an automatic algorithm and a simple interactive step. For validation, all automatically extracted areas were also manually segmented and quantified.
  
  
  RESULTS
  Statistical analysis showed significant intra- and interoperator variability in manual segmentation of all areas. Automatic quantifications did not significantly differ from mean manual evaluations of the same areas. Comparison of our image analysis quantifications with staging histologic evaluations of liver fibrosis showed significant correlations (Spearman's, 0.72<r<0.83; p<0.0001) and that the latter are based more on the distribution patterns than on the quantity of fibrosis.
  
  
  CONCLUSIONS
  FibroQuant is a sensitive, precise, objective and reproducible method of fibrosis quantification, which complements semi-quantitative histologic evaluation systems. This novel tool could be of special value in clinical trials and for improving the prognosis and follow-up among patients with fibrosis-inducing hepatic diseases.
authors.affiliation: Department of Pathology, School of Medicine and University Hospital, University of Granada, Spain. masseroli@biomed.polimi.it
authors.forename: M
authors.initials: M
authors.lastname: Masseroli
authors.forename: T
authors.initials: T
authors.lastname: Caballero
authors.forename: F
authors.initials: F
authors.lastname: O'Valle
authors.forename: R M
authors.initials: RM
authors.lastname: Del Moral
authors.forename: A
authors.initials: A
authors.lastname: Pérez-Milena
authors.forename: R G
authors.initials: RG
authors.lastname: Del Moral
mesh_terms: D000465:Algorithms; D001652:Bile Ducts / Q000473:pathology; D001808:Blood Vessels / Q000473:pathology; D005069:Evaluation Studies as Topic; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods* / Q000592:standards; D008099:Liver / Q000473:pathology*; D008103:Liver Cirrhosis / Q000473:pathology*; D011168:Portal System / Q000473:pathology