The GTP-binding protein RhoA localizes to the cortical granules of Strongylocentrotus purpuratas sea urchin egg and is secreted during fertilization.
The sea urchin egg has thousands of secretory vesicles known as cortical granules. Upon fertilization, these vesicles undergo a Ca2+-dependent exocytosis. G-protein-linked mechanisms may take place during the egg activation. In somatic cells from mammals, GTP-binding proteins of the Rho family regulate a number of cellular processes, including organization of the actin cytoskeleton. We report here that a crude membrane fraction from homogenates of Strongylocentrotus purpuratus sea urchin eggs, incubated with C3 (which ADP-ribosylates specifically Rho proteins) and [32P]NAD, displayed an [32P]ADP-ribosylated protein of 25 kDa that had the following characteristics: i) identical electrophoretic mobility in SDS-PAGE gels as the [32P]ADP-ribosylated Rho from sea urchin sperm; ii) identical mobility in isoelectro focusing gels as human RhoA; iii) positive cross-reactivity by immunoblotting with an antibody against mammalian RhoA. Thus, unfertilized S. purpuratus eggs contain a mammalian RhoA-like protein. Immunocytochemical analyses indicated that RhoA was localized preferentially to the cortical granules; this was confirmed by experiments of [32P]ADP-ribosylation with C3 in isolated cortical granules. Rho was secreted and retained in the fertilization membrane after insemination or activation with A23187. It was observed that the Rho protein present in the sea urchin sperm acrosome was also secreted during the exocytotic acrosome reaction. Thus, Rho could participate in those processes related to the cortical granules, i.e., in the Ca2+-regulated exocytosis or actin reorganization that accompany the egg activation.
Cuéllar-Mata
P
P
Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Mexico.
Martínez-Cadena
G
G
López-Godínez
J
J
Obregón
A
A
García-Soto
J
J
D000818:Animals; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure; D005306:Fertilization; D006801:Humans; D016253:Microscopy, Immunoelectron; D010063:Ovum / Q000502:physiology* / Q000648:ultrastructure; D012617:Sea Urchins / Q000502:physiology*; D020742:rhoA GTP-Binding Protein / Q000502:physiology*
D020742:rhoA GTP-Binding Protein