Human uterine myocytes retain their energy charge with No gross alterations in morphology for at least 8 days when cultured under anaerobic conditions.
OBJECTIVE
To investigate the ability of human uterine myocytes to grow under anaerobic conditions for a prolonged time period.


METHODS
Cells were isolated from fundal myometrium and cultured until subconfluency. The cell type was confirmed by immunostaining for the smooth muscle cell-specific cytoskeletal proteins alpha-actin and desmin. Some cells were further cultured under aerobic conditions and others under anaerobic conditions. Cells were harvested after 0, 4 and 8 days in culture and analyzed for their content of adenylates.


RESULTS
Immunostaining revealed that all three preparations contained almost only smooth muscle cells. Energy charge of the myocytes was 0.88 on average at the beginning of the culture experiment. A moderate decrease was noted on day 4 for myocytes grown under both aerobic and anaerobic conditions and no further decrease was noted between days 4 and 8. Morphologically the cells retained their normal appearance and they seemed healthy for at least 8 days in culture under both aerobic and anaerobic conditions.


CONCLUSIONS
The results of this study suggest that human myometrial cells can survive for an extended period of time under in vitro conditions regardless of oxygen availability for energy metabolism. This means that anaerobic energy metabolism is enough to sustain vital processes during that period of time.
Olovsson
M
M
Department of Women's and Children's Health, Section of Obstetrics and Gynecology, Uppsala University, Uppsala, Sweden. matts.olovsson@telia.com
Nordling
M
M
Ulmsten
U
U
Lindblom
B
B
Waldenström
A
A
Ronquist
G
G
D000199:Actins / Q000032:analysis; D000693:Anaerobiosis; D002478:Cells, Cultured; D003893:Desmin / Q000032:analysis; D004734:Energy Metabolism; D005260:Female; D006801:Humans; D009215:Myometrium / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D013997:Time Factors
D000199:Actins; D003893:Desmin