title: NMR structure of activated CheY.
abstract: The CheY protein is the response regulator in bacterial chemotaxis. Phosphorylation of a conserved aspartyl residue induces structural changes that convert the protein from an inactive to an active state. The short half-life of the aspartyl-phosphate has precluded detailed structural analysis of the active protein. Persistent activation of Escherichia coli CheY was achieved by complexation with beryllofluoride (BeF(3)(-)) and the structure determined by NMR spectroscopy to a backbone r.m.s.d. of 0.58(+/-0.08) A. Formation of a hydrogen bond between the Thr87 OH group and an active site acceptor, presumably Asp57.BeF(3)(-), stabilizes a coupled rearrangement of highly conserved residues, Thr87 and Tyr106, along with displacement of beta4 and H4, to yield the active state. The coupled rearrangement may be a more general mechanism for activation of receiver domains.
authors.affiliation: Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA, 94720, USA.
authors.forename: H S
authors.initials: HS
authors.lastname: Cho
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: D
authors.initials: D
authors.lastname: Yan
authors.forename: X
authors.initials: X
authors.lastname: Pan
authors.forename: J S
authors.initials: JS
authors.lastname: Parkinson
authors.forename: S
authors.initials: S
authors.lastname: Kustu
authors.forename: D E
authors.initials: DE
authors.lastname: Wemmer
authors.forename: J G
authors.initials: JG
authors.lastname: Pelton
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001608:Beryllium / Q000378:metabolism* / Q000494:pharmacology; D001665:Binding Sites; D017124:Conserved Sequence; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D005459:Fluorides / Q000378:metabolism* / Q000494:pharmacology; D006860:Hydrogen Bonding; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D000072236:Methyl-Accepting Chemotaxis Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D011487:Protein Conformation / Q000187:drug effects
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; C000607606:cheY protein, E coli; C035982:beryllium fluoride; D001608:Beryllium; D005459:Fluorides