Quantitative high-performance liquid chromatographic determination of acrolein in plasma after derivatization with Luminarin 3.
A rapid, sensitive and specific high-performance liquid chromatographic method for the quantification of acrolein (1), one of the toxic metabolites of oxazaphosphorine alkylating agents (cyclophosphamide and ifosfamide) was developed. Condensation of acrolein with Luminarin 3 afforded a fluorescent derivative that could be specifically detected and quantified. Chromatographic conditions involved a C18 RP column Uptisphere and a gradient elution system to optimize resolution and time analysis. The method showed high sensitivity with a limit of detection of 100 pmol/ml and a limit of quantification of 300 pmol/ml. This technique is particularly suitable for pharmacokinetic studies on plasma of oxazaphosphorine-receiving patients.
Paci
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A
Service de Pharmacie-Toxico-Pharmacologie, Hôpital Robert Debre, Paris, France. francoise.brion@rdb.ap-hop-paris.fr
Rieutord
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A
Guillaume
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D
Traoré
F
F
Ropenga
J
J
Husson
H P
HP
Brion
F
F
D000171:Acrolein / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011807:Quinolizines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; C409683:Luminarin 3; D011807:Quinolizines; D000171:Acrolein