title: Basal nitric oxide production is enhanced by hydraulic pressure in cultured human periodontal ligament fibroblasts.
abstract: To understand orthodontic tooth movement and determine optimal orthodontic force from a biological viewpoint, nitric oxide production in cultured human periodontal ligament fibroblasts was measured at varying levels of hydraulic pressure. The fibroblasts in a culture flask were exposed to the controlled change in hydraulic pressure, and intracellular nitric oxide levels were measured in real time by a nitric oxide-binding fluorescent dye, diaminofluorescein-2. The fibroblasts produced a significantly larger amount of nitric oxide at the pressure of 75 and 100 mmHg, compared with the pressure of 0, 25, and 50 mmHg (P <.0001, one-way ANOVA, and P <.05, Tukey-Kramer test). Immunohistochemically, the cultured fibroblasts expressed brain nitric oxide synthase. The pressure level to enhance nitric oxide production was comparable to the magnitude of clinically used orthodontic force (80 g/cm(2)). Nitric oxide might be a key regulator in orthodontic tooth movement.
authors.affiliation: Kyoyama Dental Center, Okayama City, Okayama, Japan. matsuot@cc.okayama-u.ac.jp
authors.forename: C
authors.initials: C
authors.lastname: Nakago-Matsuo
authors.forename: T
authors.initials: T
authors.lastname: Matsuo
authors.forename: T
authors.initials: T
authors.lastname: Nakago
mesh_terms: D000704:Analysis of Variance; D002469:Cell Separation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis*; D010513:Periodontal Ligament / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D011312:Pressure; D013050:Spectrometry, Fluorescence
chemical_list: D009569:Nitric Oxide