Method for rapid purification of class IIa bacteriocins and comparison of their activities.
A three-step method was developed for the purification of mesentericin Y105 (60% yield) from the culture supernatant of Leuconostoc mesenteroides Y105. The same procedure was successfully applied to the purification of five other anti-Listeria bacteriocins identified by mass spectrometry. Specific activities of the purified bacteriocins were compared.
Guyonnet
D
D
IFR-CNRS, FR 59, "Communication Cellulaire,", France.
Fremaux
C
C
Cenatiempo
Y
Y
Berjeaud
J M
JM
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D007934:Leuconostoc / Q000254:growth & development / Q000378:metabolism*; D008087:Listeria / Q000187:drug effects*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D001430:Bacteriocins; D003470:Culture Media