[Amplification and sequence of human CD14 gene from human mononuclear cell in peripheral blood].
OBJECTIVE
To introduce a new method for getting human CD14 gene.


METHODS
Based on the character of CD14 gene, the authors amplified the CD14 gene directly from genome DNA of human mononuclear cell in peripheral blood by PCR for the first time.


RESULTS
A human CD14 gene about 1.1 kb was successfully amplified from genome DNA of human mononuclear cell in peripheral blood, and the sequence analysis has indicated that the CD14 gene cloned in this way is the same as the sequence reported by other authors.


CONCLUSIONS
It is a simple and effective method to obtain human CD14 gene from genome DNA of human mononuclear cell in peripheral blood.
Zhang
W
W
Research Unit of Infection & Immunity, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P.R. China.
Bao
L
L
Qiu
H
H
Yan
J
J
Hu
C
C
Zhang
H
H
D001483:Base Sequence; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016133:Polymerase Chain Reaction
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides