title: Shigella actin-based motility in the presence of truncated vinculin.
abstract: Mounting evidence supports the role of truncated vinculin in the intracellular actin-based motility of Shigella flexneri. Vinculin's role was recently questioned by Goldberg [1997: Cell Motil Cytoskeleton 37:44-53] who observed Shigella motility in mouse embryonal carcinoma 5.51 cells, a genetically modified cell line that reputedly lacked vinculin. That challenge implicitly relied on the assumption that 5.51 cells had no detectable vinculin polypeptide and lacked full-length vinculin mRNA. Despite the appearance of being an unambiguous test of vinculin's role in Shigella motility, 5.51 cells were shown to contain adequate amounts of truncated vinculin (as well as the corresponding mRNA transcript) to support bacterial locomotion. We also examined Shigella locomotion in gamma229 cells, a related embryonal carcinoma cell line containing approximately one-half the vinculin content found in 5.51 cells. We observed that there was a commensurate twofold decrease in the Shigella motility rate, as compared to 5.51 cells; this finding raises the possibility that vinculin can become a rate-limiting factor under some circumstances. Immunofluorescence microscopy using vin 11-5 monoclonal antibody directed against the vinculin head domain showed intense staining of Shigella rocket tails in both gamma229 and 5.51 cells. Our findings clearly demonstrate that motility in 5.51 cells cannot be regarded as a valid criterion for evaluating the role of truncated vinculin in Shigella motility.
authors.affiliation: Department of Medicine, University of Florida College of Medicine, Health Science Center, Gainesville 32610, USA. Southfs@medmac.ufl.edu
authors.forename: F S
authors.initials: FS
authors.lastname: Southwick
authors.forename: E D
authors.initials: ED
authors.lastname: Adamson
authors.forename: D L
authors.initials: DL
authors.lastname: Purich
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement; D002474:Cell-Free System; D051379:Mice; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012763:Shigella flexneri / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D016596:Vinculin / Q000494:pharmacology / Q000502:physiology*
chemical_list: D000199:Actins; D016596:Vinculin