title: Alteration in the radiation-induced LD release in HeLa cells by acyclovir.
abstract: The aim of the present investigation was to study the effect of various concentrations of acyclovir (ACV) alone or the effect of 0.1 micromol/l acycloir pretreatment on the radion-induced lactate dehydrogenase (LD) release by HeLa cells exposed to 0-3 Gy of gamma-radiation. The plateau-phase cells were treated with 0-100 micromol/l ACV for 8 h or 0.1 micromol/l ACV before exposure to 0-3 Gy of gamma-radiation. The whole media was removed from the culture and the LD release was measured at 0, 0.5, 1, 2, and 4 h post-drug treatment or post-irradiation. The ACV treatment resulted in a concentration-dependent elevation in the LD release at all the post-treatment time periods. A peak level for LD release was observed at 0 h up to 1 micromol/l ACV and declined thereafter. However, at higher doses a highest elevation in LD release was observed at 1 h post-treatment. Pretreatment of HeLa cells with 0.1 micromol/l ACV before irradiation to different doses of gamma-radiation resulted in significant elevation in the release of LD in the medium. The LD release was twofold greater in the ACV+irradiation group at 0 h post-irridiation when compared with the concurrent PBS+gamma-radiation group. The highest increase in LD activity was observed at 0 h post-irradiation and a gradual decline thereafter, without restoration to non-drug treated control level up to 4 h post-irradiation. The LD measurements can serve as a short-term assay to evaluate the cytotoxicity of ACV or radiation in vitro.
authors.affiliation: Department of Radiobiology, Kasturba Medical College, Manipal, India. info@kmc.ernet.in
authors.forename: G C
authors.initials: GC
authors.lastname: Jagetia
authors.forename: R
authors.initials: R
authors.lastname: Aruna
authors.forename: B S
authors.initials: BS
authors.lastname: Nayak
mesh_terms: D000212:Acyclovir / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D007770:L-Lactate Dehydrogenase; D000212:Acyclovir