title: Characterization of L-carnitine transport into rat skeletal muscle plasma membrane vesicles.
abstract: Transport of L-carnitine into skeletal muscle was investigated using rat sarcolemmal membrane vesicles. In the presence of an inwardly directed sodium chloride gradient, L-carnitine transport showed a clear overshoot. The uptake of L-carnitine was increased, when vesicles were preloaded with potassium. When sodium was replaced by lithium or cesium, and chloride by nitrate or thiocyanate, transport activities were not different from in the presence of sodium chloride. However, L-carnitine transport was clearly lower in the presence of sulfate or gluconate, suggesting potential-dependent transport. An osmolarity plot revealed a positive slope and a significant intercept, indicating transport of L-carnitine into the vesicle lumen and binding to the vesicle membrane. Displacement experiments revealed that approximately 30% of the L-carnitine associated with the vesicles was bound to the outer and 30% to the inner surface of the vesicle membrane, whereas 40% was unbound inside the vesicle. Saturable transport could be described by Michaelis-Menten kinetics with an apparent Km of 13.1 microM and a Vmax of 2.1 pmol.(mg protein-1).s-1. L-Carnitine transport could be trans-stimulated by preloading the vesicles with L-carnitine but not with the carnitine precursor butyrobetaine, and was cis-inhibited by L-palmitoylcarnitine, L-isovalerylcarnitine, and glycinebetaine. On comparing carnitine transport into rat kidney brush-border membrane vesicles and OCTN2, a sodium-dependent high-affinity human carnitine transporter, cloned recently from human kidney also expressed in muscle, the Km values are similar but driving forces, pattern of inhibition and stereospecificity are different. This suggests the existence of more than one carnitine carrier in skeletal muscle.
authors.affiliation: Division of Clinical Pharmacology and Toxicology, Department of Internal Medicine, University Hospital, Swiss Federal Institute of Technology, Biochemie III, Zürich, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Berardi
authors.forename: B
authors.initials: B
authors.lastname: Stieger
authors.forename: B
authors.initials: B
authors.lastname: Hagenbuch
authors.forename: E
authors.initials: E
authors.lastname: Carafoli
authors.forename: S
authors.initials: S
authors.lastname: Krähenbühl
mesh_terms: D000818:Animals; D001692:Biological Transport; D002331:Carnitine / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008564:Membrane Potentials; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014982:Xenopus laevis
chemical_list: D002331:Carnitine