Camel (camelus dromedarius) milk PP3: evidence for an insertion in the amino-terminal sequence of the camel milk whey protein.
The camel (camelus dromedarius) milk proteose peptone 3 (PP3) was purified successively by size exclusion fast protein liquid chromatography and reversed phase high performance liquid chromatography and then characterized by amino acid residue composition determination and chemical microsequencing after CNBr or trypsin cleavages. In comparison with the previously reported structure of camel milk whey protein, the camel PP3 contains an insertion in the N-terminal region which has approximately 24 residues, whereas the remaining C-terminal regions of these two homologous proteins are essentially identical. The camel PP3 seems to contain a potential O-glycosylation site localized in this insertion and 2 or 3 phosphorylated serine residues. PP3 belongs to the glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) family and could therefore play an immunological role in the camel or its suckling young.
Girardet
J M
JM
Laboratoire des BioSciences de l'Aliment, Université Henri Poincaré-Nancy, Vandoeuvre-lès-Nancy, France. Jean-Michel.Girardet@scbiol.uhp-nancy.fr
Saulnier
F
F
Gaillard
J L
JL
Ramet
J P
JP
Humbert
G
G
D000595:Amino Acid Sequence; D000818:Animals; D002162:Camelus; D002364:Caseins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006023:Glycoproteins / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010449:Peptide Mapping; D020539:Sequence Analysis, Protein; D000067816:Whey Proteins
D002364:Caseins; D006023:Glycoproteins; D008894:Milk Proteins; C085522:PP3 protein, Bos taurus; D010446:Peptide Fragments; D000067816:Whey Proteins