title: Semi-automated 96-well liquid-liquid extraction for quantitation of drugs in biological fluids.
abstract: A semi-automated liquid-liquid extraction (LLE) technique for biological fluid sample preparation was introduced for the quantitation of four drugs in rat plasma. All liquid transferring during the sample preparation was automated using a Tomtec Quadra 96 Model 320 liquid handling robot, which processed up to 96 samples in parallel. The samples were either in 96-deep-well plate or tube-rack format. One plate of samples can be prepared in approximately 1.5 h, and the 96-well plate is directly compatible with the autosampler of an LC/MS system. Selection of organic solvents and recoveries are discussed. Also, precision, relative error, linearity and quantitation of the semi automated LLE method are estimated for four example drugs using LC/MS/MS with a multiple reaction monitoring (MRM) approach. The applicability of this method and future directions are evaluated.
authors.affiliation: Department of Pharmacokinetics, Dynamics and Metabolism, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert, Ann Arbor, MI 48105, USA.
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: K L
authors.initials: KL
authors.lastname: Hoffman
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: D T
authors.initials: DT
authors.lastname: Rossi
mesh_terms: D000465:Algorithms; D000818:Animals; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008838:Microcomputers; D004364:Pharmaceutical Preparations / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D012997:Solvents
chemical_list: D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D012997:Solvents