Altered ischemic induction of immediate early gene and heat shock protein 70 mRNAs after preconditioning in rat hearts.
Immediate early genes and heat shock protein (HSP) 70s, which may play a role in adaptation and cellular protection, respectively, are induced by ischemia in hearts. We examined if the induction of immediate early gene (c-fos, c-myc, c-jun, and junB) and HSP70 mRNAs by ischemia is affected by ischemic preconditioning. Transient ischemia (5 or 10 minute) was applied to Wistar rat (n=75) hearts, by tightening a snare placed around left coronary arterial branches 7 days before applying ischemia. Rats without earlier ischemia (control group, C) and rats with 5-minute ischemia 12 or 24 hours earlier (EI12 or 24 group) were given 10-minute ischemia and sacrificed at 0, 0.5, 1, 2, and 4 hour. RNA was extracted from the ischemic region and Northern blot analysis was performed. The induction of c-fos and c-myc mRNAs was significantly increased in EI12 but not in EI24 compared with that in C. The induction of c-jun and junB mRNAs showed no change in both EI12 and EI24 compared with that in C. The induction of HSP72 and 73 mRNAs was decreased in EI12 and decreased further in EI24. Thus, ischemic preconditioning altered the induction of immediate early gene and HSP70 mRNAs by ischemia. The effect of preconditioning differed among genes studied and changed with time after preconditioning. Ischemic preconditioning alters protective and adaptive responses to ischemia at the gene level.
Nitta-Komatsubara
Y
Y
Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, Japan.
Abe
K
K
Aoki
M
M
Isoyama
S
S
D000222:Adaptation, Physiological / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D017781:Genes, Immediate-Early / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger