Semi-automated 96-well liquid-liquid extraction for quantitation of drugs in biological fluids.
A semi-automated liquid-liquid extraction (LLE) technique for biological fluid sample preparation was introduced for the quantitation of four drugs in rat plasma. All liquid transferring during the sample preparation was automated using a Tomtec Quadra 96 Model 320 liquid handling robot, which processed up to 96 samples in parallel. The samples were either in 96-deep-well plate or tube-rack format. One plate of samples can be prepared in approximately 1.5 h, and the 96-well plate is directly compatible with the autosampler of an LC/MS system. Selection of organic solvents and recoveries are discussed. Also, precision, relative error, linearity and quantitation of the semi automated LLE method are estimated for four example drugs using LC/MS/MS with a multiple reaction monitoring (MRM) approach. The applicability of this method and future directions are evaluated.
Zhang
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Department of Pharmacokinetics, Dynamics and Metabolism, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert, Ann Arbor, MI 48105, USA.
Hoffman
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KL
Li
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Rossi
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DT
D000465:Algorithms; D000818:Animals; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008838:Microcomputers; D004364:Pharmaceutical Preparations / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D012997:Solvents
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D012997:Solvents