Utility and quality of autologous fresh frozen plasma and autologous fibrin glue for surgical patients.
The quality of autologous fresh frozen plasma and autologous fibrin glue prepared in our hospital was evaluated. The Auto-FFP was separated from whole blood or collected using a cell separator and the Auto-FG was then prepared from the Auto-FFP. The Auto-FFP showed significantly higher levels of fibrinogen, Factor-V and Factor-VIII than commercially available FFP. The Auto-FG contained approximately 10 times the concentration of fibrinogen, Factor-VIII, Factor-XIII, von Willbrand factor and fibronectin and 1.5 times that of alpha 2-Plasminogen inhibitor compared to those of the Auto-FFP. When the Auto-FG was sprayed over diffusely bleeding sites from surgical wounds, hemostasis occurred within 5 s. These results suggest that Auto-FFP and Auto-FG may have sufficient utility and quality to reduce the use of allogeneic blood products.
Komatsu
F
F
Blood Transfusion Service, School of Medicine, Tokyo Medical and Dental University, Japan.
Yoshida
S
S
D000328:Adult; D000368:Aged; D001779:Blood Coagulation Factors / Q000032:analysis; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D001798:Blood Proteins / Q000032:analysis; D001804:Blood Transfusion, Autologous / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005260:Female; D005337:Fibrin / Q000302:isolation & purification; D005340:Fibrinogen / Q000032:analysis; D006488:Hemostasis, Surgical / Q000379:methods*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D010949:Plasma; D012189:Retrospective Studies; D013997:Time Factors; D014014:Tissue Adhesives
D001779:Blood Coagulation Factors; D001798:Blood Proteins; D014014:Tissue Adhesives; D005337:Fibrin; D005340:Fibrinogen