Characterization of the genetic locus responsible for production and immunity of carnobacteriocin A: the immunity gene confers cross-protection to enterocin B.
Carnobacteriocin A (CbnA) is a regulated bacteriocin produced by Carnobacterium piscicola LV17A that is encoded on a 72 kb plasmid. A 10.0 kb fragment from this plasmid that contained information necessary for bacteriocin production and immunity was cloned and sequenced. Genetic analysis showed the presence of the previously sequenced structural gene for CbnA, as well as genes encoding proteins homologous to dedicated bacteriocin transport proteins and proteins of three-component signal transduction systems. The induction factor (CbnX) was chemically synthesized and induced CbnA production at 10(-11) M or higher in a C. piscicola LV17A culture that had lost the ability to produce bacteriocin as a result of dilution. The gene cbiA for the immunity protein is not located in typical close proximity to the structural gene for CbnA and is encoded in the opposite orientation. CbiA has homology with EniB, the immunity protein for enterocin B that is also encoded in the opposite orientation to the bacteriocin gene. CbiA and EniB cross-protected against the corresponding bacteriocins.
Franz
Charles M A P
CMAP
Departments of Agricultural, Food and Nutritional Science1 and Chemistry2, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
van Belkum
Marco J
MJ
Departments of Agricultural, Food and Nutritional Science1 and Chemistry2, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
Worobo
Randy W
RW
Departments of Agricultural, Food and Nutritional Science1 and Chemistry2, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
Vederas
John C
JC
Departments of Agricultural, Food and Nutritional Science1 and Chemistry2, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
Stiles
Michael E
ME
Departments of Agricultural, Food and Nutritional Science1 and Chemistry2, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007777:Lactobacillaceae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins; D015398:Signal Transduction
D001426:Bacterial Proteins; D001430:Bacteriocins; C409491:CbiA protein, Carnobacterium piscicola; D011993:Recombinant Fusion Proteins; C093812:dvnA protein, Carnobacterium divergens; C107799:enterocin A; C107801:enterocin B; C087892:cbnBA protein, Carnobacterium piscicola