title: A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NoIL in Rhizobium leguminosarum.
abstract: Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).
authors.affiliation: Leiden University, Institute of Molecular Plant Sciences, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Pacios Bras
authors.forename: M A
authors.initials: MA
authors.lastname: Jordá
authors.forename: A H
authors.initials: AH
authors.lastname: Wijfjes
authors.forename: M
authors.initials: M
authors.lastname: Harteveld
authors.forename: N
authors.initials: N
authors.lastname: Stuurman
authors.forename: J E
authors.initials: JE
authors.lastname: Thomas-Oates
authors.forename: H P
authors.initials: HP
authors.lastname: Spaink
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D001426:Bacterial Proteins; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000302:isolation & purification; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000382:microbiology*; D016961:Rhizobium leguminosarum / Q000235:genetics / Q000378:metabolism*; D013559:Symbiosis / Q000235:genetics* / Q000502:physiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D010940:Plant Proteins; C023023:lipid-linked oligosaccharides; C117779:noIL protein, Rhizobium etli; D005647:Fucosyltransferases; C103941:NodZ protein, bacteria