title: Morphofunctional plasticity in the adult hypothalamus induces regulation of polysialic acid-neural cell adhesion molecule through changing activity and expression levels of polysialyltransferases.
abstract: Polysialic acid-neural cell adhesion molecule (PSA-NCAM) expression in the adult nervous system is restricted to regions retaining a capacity for morphological plasticity. For the female rat hypothalamoneurohypophysial system (HNS), we have previously shown that lactation induces a dramatic decrease in PSA-NCAM, while leaving the level of total NCAM protein unchanged. Here, we wanted to elucidate the molecular mechanisms leading to a downregulation of PSA, thereby stabilizing newly established synapses and neurohemal contacts that accompany the increased activity of oxytocinergic neurons. First, we show that the overall specific activity of polysialyltransferases present in tissue extracts from supraoptic nuclei decreases by approximately 50% during lactation. So far, two polysialyltransferase enzymes, STX and PST, have been characterized for their capacity to transfer PSA onto NCAM in vitro. Using a competitive RT-PCR on RNA extracts from the HNS, we demonstrate furthermore a significant decrease in the expression levels of both STX and PST mRNAs in lactating versus virgin animals. Interestingly, this downregulation of NCAM polysialylation is not correlated with the post-transcriptional regulation of variable alternative spliced exon splicing, in contrast to neural development. The control of polysialylation via a regulation of both enzyme activity and expression underlines the important role of this post-translational modification of NCAM in morphofunctional plasticity in adult brain.
authors.affiliation: Institut Alfred Fessard, Centre National de la Recherche Scientifique UPR 2212, Gif sur Yvette, 91198, France.
authors.forename: S
authors.initials: S
authors.lastname: Soares
authors.forename: Y
authors.initials: Y
authors.lastname: von Boxberg
authors.forename: M
authors.initials: M
authors.lastname: Ravaille-Veron
authors.forename: J D
authors.initials: JD
authors.lastname: Vincent
authors.forename: F
authors.initials: F
authors.lastname: Nothias
mesh_terms: D000818:Animals; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007031:Hypothalamus / Q000502:physiology*; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology*; D009842:Oligopeptides / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000378:metabolism*
chemical_list: D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D009842:Oligopeptides; D012333:RNA, Messenger; D012794:Sialic Acids; C086869:VASE peptide; C103469:polysialyl neural cell adhesion molecule