Influenza A virus-binding activity of glycoglycerolipids of aquatic bacteria.
As the aqueous sphere has been proposed to be an important source medium for the virus infection of land animals, the glycolipids of some aquatic organisms were examined for human influenza A virus-binding activity. Active compounds were not found among the eight echinoderm gangliosides, but two active non-sialylated glycoglycerolipids were isolated from an aquatic bacterium, Corynebacterium aquaticum. The structural formula of one of them, H632A, was elucidated to be 1-14-methyl-hexadecanoyl-3-alpha-D-galactopyranosyl-(1-->3)-6-(12-met hyl-tetradecanoyl)-1-alpha-D-mannopyranosyl]-sn-glycerol. The latter together with reported one elsewhere, S365A, 1-14-methyl-hexadecanoyl-3-[alpha-D-mannopyranosyl-(1-->3)-6-(12-meth yl-tetradecanoyl)-1-alpha-D-mannopyranosyl]-sn-glycerol, apparently bound to three human influenza viruses, A/PR/8/34 (H1N1), A/Aichi/2/68 (H3N2), and A/Memphis/1/71 (H3N2), exhibiting 7-12% (H632A) and 10-22% (S365A) of the activities of the control substances (Neu5Acalpha2-3-paragloboside and Neu5Acalpha2-6- paragloboside). Additionally, these glycolipids were assumed to have virus-neutralizing activities for the following two reasons: (i) The hemagglutination and hemolysis activities of the viruses were inhibited by the glycolipid. (ii) The leakage of a cytosolic enzyme (lactate dehydrogenase) from Madin-Darby canine kidney cells on virus infection was prevented by the glycolipids to nearly the same extent as by fetuin. This is the first evidence of the binding- and neutralizing-abilities of native glycoglycerolipids as to influenza viruses.
Nakata
K
K
Central Research Laboratories, Mercian Corporation, Johnan, Fujisawa 251-0057, Japan. knakata@mb.infoweb.ne.jp.
Guo
C T
CT
Matsufuji
M
M
Yoshimoto
A
A
Inagaki
M
M
Higuchi
R
R
Suzuki
Y
Y
D000818:Animals; D001419:Bacteria / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D002240:Carbohydrate Sequence; D002460:Cell Line; D003352:Corynebacterium / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D004285:Dogs; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D006386:Hemagglutination Tests; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D009980:Influenza A virus / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000821:virology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D014871:Water Microbiology
D006017:Glycolipids; D007770:L-Lactate Dehydrogenase