Characterization of pig connective tissue growth factor (CTGF) cDNA, mRNA and protein from uterine tissue.
Connective tissue growth factor (CTGF) is a 38kDa mitogen and chemotactic factor for fibroblasts that is transcriptionally activated by serum or transforming growth factor-beta and may play a role in wound healing and various skin diseases. In these studies, pig endometrium was shown to contain a single CTGF transcript of 2.4kb and to produce a 38kDa CTGF-immunoreactive protein. Cloning and sequencing of a 1.5kb pig uterine CTGF cDNA revealed that the predicted pCTGF primary translation product displayed 92% identity to human CTGF and 93% identity to mouse CTGF. The pCTGF cDNA encoded a 26 amino acid signal peptide followed by a 323-residue sequence containing 38 highly conserved cysteine residues. In common with mouse and human CTGF proteins, pCTGF is predicted to resemble a multi-functional mosaic protein that contains four distinct modules.
Harding
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Department of Surgery, The Ohio State University and Children's Hospital, Columbus 43205, USA.
Surveyor
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Brigstock
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D055513:Connective Tissue Growth Factor; D018076:DNA, Complementary / Q000235:genetics*; D004717:Endometrium / Q000737:chemistry*; D005260:Female; D006133:Growth Substances / Q000737:chemistry / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics*; D016415:Sequence Alignment; D013552:Swine
C526317:CCN2 protein, human; C526318:CCN2 protein, mouse; D018076:DNA, Complementary; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor