title: A conserved "hydrophobic staple motif" plays a crucial role in the refolding of human glutathione transferase P1-1.
abstract: The specific (i, i+5) hydrophobic staple interaction involving a helix residue and a second residue located in the turn preceding the helix is a recurrent motif at the N terminus of alpha-helices. This motif is strictly conserved in the core of all soluble glutathione transferases (GSTs) as well as in other protein structures. Human GSTP1-1 variants mutated in amino acid Ile(149) and Tyr(154) of the hydrophobic staple motif of the alpha6-helix were analyzed. In particular, a double mutant cycle analysis has been performed to evaluate the role of the hydrophobic staple motif in the refolding process. The results show that this local interaction, by restricting the number of conformations of the alpha6-helix relative to the alpha1-helix, favors the formation of essential interdomain interactions and thereby accelerates the folding process. Thus, for the first time it is shown that the hydrophobic staple interaction has a role in the folding process of an intact protein. In P(i) class GSTs, Tyr(154) appears to be of particular structural importance, since it interacts with conserved residues Leu(21), Asp(24), and Gln(25) of the adjacent alpha1-helix which contributes to the active site. Human GSTP1-1 variants L21A and Y154F have also been analyzed in order to distinguish the role of interdomain interactions from that of the hydrophobic staple. The experimental results reported here suggest that the strict conservation of the hydrophobic staple motif reflects an evolutionary pressure for proteins to fold rapidly.
authors.affiliation: Department of Biochemistry, Uppsala University, Biomedical Center, Box 576, S-751 23 Uppsala, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Stenberg
authors.forename: B
authors.initials: B
authors.lastname: Dragani
authors.forename: R
authors.initials: R
authors.lastname: Cocco
authors.forename: B
authors.initials: B
authors.lastname: Mannervik
authors.forename: A
authors.initials: A
authors.lastname: Aceto
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D003198:Computer Simulation; D017124:Conserved Sequence; D004795:Enzyme Stability; D014644:Genetic Variation; D005982:Glutathione Transferase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D007532:Isoleucine; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics; D014443:Tyrosine
chemical_list: D007527:Isoenzymes; D011994:Recombinant Proteins; D007532:Isoleucine; D014443:Tyrosine; D005982:Glutathione Transferase