title: Blood profile during and after hemoglobin substitute administration.
abstract: The in vivo effects of Diaspirin Crosslinked Hemoglobin (DCLHb, Baxter Healthcare Corp.) on hematology and biochemistry are unknown. This study includes 6 calves (71.2+/-1.3 kg). In each animal a total of 2 litres of blood was exchanged for the same amount of hydroxylethyl starch (Haes, Fresenius) (n=3) or DCLHb (n=3), which is equivalent to 28cc/kg of blood substitute, over a period of 5 hours. The animals were allowed to survive 7 days. Blood samples were taken hourly during the perfusion protocol, at postoperative day (POD) 1, 2 and 7. ANOVA test was used for repeated measurements. Blood cell profiles were similar in both groups. Peak methemoglobinemia was 4.2% in the DCLHb group. Osmolarity was significantly higher in the DCLHb group with the greatest difference at POD 1 and 2. Postmortem analysis of the major organs did not show any sign of hemoglobin deposit in the DCLHb group. In the given setup DCLHb can be administered in a large quantity with good hematological tolerance and without any deposits in major organs. A prolonged plasma expander effect was observed.
authors.affiliation: Clinic for Cardiovascular Surgery, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors.forename: X M
authors.initials: XM
authors.lastname: Mueller
authors.forename: H T
authors.initials: HT
authors.lastname: Tevaearai
authors.forename: J P
authors.initials: JP
authors.lastname: Gardaz
authors.forename: D
authors.initials: D
authors.lastname: Jegger
authors.forename: L K
authors.initials: LK
authors.lastname: von Segesser
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives* / Q000494:pharmacology; D001772:Blood Cell Count; D001802:Blood Substitutes / Q000494:pharmacology*; D002315:Cardiopulmonary Bypass; D002417:Cattle; D006454:Hemoglobins / Q000494:pharmacology*; D008708:Methemoglobinemia / Q000209:etiology; D009994:Osmolar Concentration
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin