Atypical clustering of gynecologic malignancies: A family study including molecular analysis of candidate genes.
OBJECTIVE
We set out to determine whether hereditary nonpolyposis colorectal cancer (HNPCC) was responsible for cancer susceptibility in a family with gynecologic malignancies in three consecutive generations.


METHODS
A detailed family history study, including review of medical records, was undertaken. Tumor DNAs from affected family members were evaluated for microsatellite instability (MSI). Linkage between cancer susceptibility and the candidate DNA mismatch repair genes MLH1, MSH2, MSH3, and MSH6 (GTBP) was investigated. MLH1 and MSH2 protein expression was evaluated by immunohistochemistry and MSH2 was investigated for mutation.


RESULTS
Four gynecologic malignancies in the core family were confirmed. MSI was seen in six of seven cancers studied. The only MSI-negative tumor was an ovarian cancer from the proband's maternal grandmother, which arose at the age of 92. Haplotype analysis using chromosome 2p markers implicated the MSH2 gene in this family's cancer susceptibility. MSH2 protein expression was absent in an MSI-positive colon cancer from an affected family member.


CONCLUSIONS
The inability to exclude linkage of MSH2 with the disease susceptibility, the presence of the MSI phenotype in cancers from family members sharing the same region of chromosome 2p, and the lack of immunodetectable MSH2 point to MSH2-associated HNPCC as a cause for this family's cancer susceptibility. Continued efforts to increase awareness of the heritability of endometrial cancer should improve our understanding of the disease, with resultant improved surveillance strategies, recommendations for surgical and chemoprophylaxis, and identification of patients at risk for malignancy as a result of HNPCC.
Cohn
D E
DE
Division of Gynecologic Oncology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. cohnd@msnotes.wustl.edu
Babb
S
S
Whelan
A J
AJ
Mutch
D G
DG
Herzog
T J
TJ
Rader
J S
JS
Elbendary
A
A
Goodfellow
P J
PJ
D000328:Adult; D000368:Aged; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D015179:Colorectal Neoplasms / Q000150:complications / Q000235:genetics*; D004252:DNA Mutational Analysis; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D005833:Genital Neoplasms, Female / Q000209:etiology / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D010375:Pedigree; D010641:Phenotype
D004273:DNA, Neoplasm