title: Comparison of in vitro activity of danofloxacin, florfenicol, oxytetracycline, spectinomycin and tilmicosin against Mycoplasma mycoides subspecies mycoides small colony type.
abstract: Minimum inhibitory concentrations (MIC) and minimum mycoplasmacidal concentrations (MMC) of the antimicrobials danofloxacin, florfenicol, oxytetracycline, spectinomycin and tilmicosin were determined in vitro for 20 isolates of Mycoplasma mycoides subspecies mycoides small colony type (MmmSC), the causative agent of contagious bovine pleuropneumonia (CBPP). The majority of strains were most susceptible to tilmicosin, followed by danofloxacin, oxytetracycline, florfenicol and spectinomycin with MIC50 values of 0.015, 0.25, 0.5, 1 and 8 microg/ml, and MMC50 values of 0.06, 0.5, 8, 8 and 16 microg/ml, respectively. However, tilmicosin had poor mycoplasmacidal activity against two recent strains from Portugal. There was no evidence of resistance to danofloxacin in any of the strains.
authors.affiliation: Veterinary Laboratories Agency (Weybridge), Addlestone, Surrey.
authors.forename: R D
authors.initials: RD
authors.lastname: Ayling
authors.forename: S E
authors.initials: SE
authors.lastname: Baker
authors.forename: R A
authors.initials: RA
authors.lastname: Nicholas
authors.forename: M L
authors.initials: ML
authors.lastname: Peek
authors.forename: A J
authors.initials: AJ
authors.lastname: Simon
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002418:Cattle Diseases / Q000188:drug therapy / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D024841:Fluoroquinolones; D018942:Macrolides; D009176:Mycoplasma mycoides / Q000187:drug effects*; D010118:Oxytetracycline / Q000494:pharmacology; D011002:Pleuropneumonia, Contagious / Q000188:drug therapy*; D000198:Spectinomycin / Q000494:pharmacology; D013839:Thiamphenicol / Q000031:analogs & derivatives / Q000494:pharmacology; D015645:Tylosin / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D018942:Macrolides; C068581:danofloxacin; D000198:Spectinomycin; C035534:florfenicol; D013839:Thiamphenicol; D010118:Oxytetracycline; C052319:tilmicosin; D015645:Tylosin