Analysis of cis-trans isomers and enantiomers of sertraline by cyclodextrin-modified micellar electrokinetic chromatography.
In this work development, optimization and validation of a cyclodextrin-modified micellar electrokinetic chromatography (CD-modified MEKC) method is proposed to resolve separation of the sertraline hydrochloride and synthesis-related substances. Sertraline hydrochloride, the cis-(1S,4S) enantiomer form, is used as an antidepressant therapeutic agent. A buffer concentration composed of 20 mM sodium borate, pH 9.0 with 50 mM sodium cholate, 15 mM sulfated beta-cyclodextrin and 5 mM hydroxypropyl-beta-cyclodextrin was found to be the most suitable background electrolyte. Quantitation of the impurities at levels of 0.1% in different samples of the bulk drug was determined. A comparison of the results with those obtained by HPLC methodology was also accomplished. The method proved appropriate for testing the purity of sertraline hydrochloride in bulk drug.
Lucangioli
S E
SE
Faculty of Pharmacy and Biochemistry, Department of Analytical Chemistry and Physicochemistry, University of Buenos Aires, Junín, Argentina.
Hermida
L G
LG
Tripodi
V P
VP
Rodríguez
V P
VP
López
E E
EE
Rouge
P D
PD
Carducci
C N
CN
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D007536:Isomerism; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis* / Q000737:chemistry; D020280:Sertraline / Q000032:analysis* / Q000737:chemistry
D003505:Cyclodextrins; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline