Expression of plant group 2 and group 3 lea genes in Saccharomyces cerevisiae revealed functional divergence among LEA proteins.
To study functions of late embryogenesis abundant (LEA) proteins, which accumulate in plant cells under water deficit conditions, in vivo functional analyses were carried out using a yeast (Saccharomyces cerevisiae) heterologous expression system. Two lea genes, tomato le4 (group 2) and barley HVA1 (group 3), were expressed under the GAL1 promoter, and the gene products were detected using specific antisera. The growth of the transformants was scored and compared with a control strain to analyze the effect of these proteins on yeast cells under stress conditions. The yeast cells expressing HVA1 showed shorter lag period when transferred to a medium containing 1.2 M NaCl as compared to a control strain, while the cells expressing le4 did not show improved growth. Attenuated growth inhibition in a medium containing 1.2 M KCl was observed in the yeast cells expressing le4 and HVA1. No obvious growth improvement was observed in a high sorbitol medium in the cells expressing either le4 or HVA1. Increased freezing tolerance was observed in both lea-expressing cells, while no effect on heat tolerance was observed. These results support the hypothesis that different LEA proteins play a distinctive role in the protection against cellular dehydration.
Zhang
L
L
Department of Biotechnology, University of Tokyo, Yayoi, Japan.
Ohta
A
A
Takagi
M
M
Imai
R
R
D005615:Freezing; D005690:Galactose / Q000494:pharmacology; D015870:Gene Expression; D017343:Genes, Plant; D006360:Heat-Shock Proteins / Q000378:metabolism; D001467:Hordeum / Q000235:genetics; D006358:Hot Temperature; D018551:Solanum lycopersicum / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
C090398:HVA1 protein, Hordeum vulgare; D006360:Heat-Shock Proteins; D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; D005690:Galactose