Use of a micro-expanded bed containing immobilised lysozyme for cell disruption in flow injection analysis.
A method for cell disruption in Flow Injection Analysis (FIA) systems has been developed. The principle involves on-line cell disruption by means of immobilised lysozyme followed by an ultrasonic treatment. In order to avoid flow problems in the analytical system, the lysozyme was immobilised to Streamline that was used in an expanded bed in the flow system. Samples of suspensions of Micrococcus lysodeikticus were treated and the success of the treatment was evaluated in terms of released protein and as a decrease in the optical density at 450 nm. The new technology offers a powerful tool in flow injection analyses for quantification of intracellular compounds. The concept of integration, i.e. combining cell disruption with handling of cell debris and assay procedure in one continuous flow process facilitates its use and increases the probability of reaching reproducible and reliable results.
Nandakumar
M P
MP
Department of Biotechnology, Lund University, Sweden.
Tocaj
A
A
Mattiasson
B
B
D004800:Enzymes, Immobilized / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods*; D008837:Micrococcus / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry*
D004800:Enzymes, Immobilized; D009113:Muramidase