NADPH dependent superoxide generation in the ovary and uterus of mice during estrous cycle and early pregnancy.
NADPH dependent O2- production in the ovary and uterus of cycling and pregnant mice was estimated employing a novel technique of time-lapse electron spin resonance enhancement of a superoxide-trapping spin trap. The NADPH dependent O2- generation in the mouse ovary increased during the early pre-ovulatory phase in cycling females and during extended luteal phase in pregnant animals. A peak of uterine NADPH dependent O2- production at proestrous in the cycling animals at pre-implantation phase in pregnant animals suggested a contribution of this enzyme towards generating high levels of superoxide anion radical during the respective stages. Both ovarian and uterine NADPH dependent O2- production appeared to be LH-inducible.
Jain
S
S
School of Life Sciences, Devi Ahilya University, Vigyan Bhawan, Indore, India.
Saxena
D
D
Kumar
G P
GP
Laloraya
M
M
D000818:Animals; D004050:Ditiocarb / Q000494:pharmacology; D004971:Estrus / Q000502:physiology*; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology; D051379:Mice; D009249:NADP / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011359:Proestrus / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D013481:Superoxides; D009249:NADP; D007986:Luteinizing Hormone; D004050:Ditiocarb; D013482:Superoxide Dismutase