Kinetics of excision repair of UV-induced DNA damage, measured using the comet assay.
The kinetics of UV- (254 nm) irradiation-induced DNA single-strand breaks (SSBs), generated during the excision repair of UV-induced DNA damage, in leukemic lymphocytes and in normal blood mononuclear cells (MNCs) were studied using the alkaline comet assay. The cells were isolated by density gradient centrifugation from peripheral blood of patients with chronic lymphocytic leukemia (CLL) and from healthy study subjects. The cytotoxicity of UV irradiation was determined in vitro in peripheral blood mononuclear lymphocytes from 36 CLL patients and from eight healthy donors using the incorporation of radioactive leucine in 4-day cultures. A remarkable difference in excision repair capability was observed between normal and leukemic lymphocytes. In contrast to normal lymphocytes, there was always a subpopulation of CLL cells that did not complete the repair of UV-induced DNA damage during the 24-h repair period. Furthermore, differences were also recorded between UV-sensitive and UV-resistant CLL cases. The differences in DNA migration between the maximum increase (59-77 microm) and that at 24 h after irradiation (21-66 microm) was statistically significant in two of three patients exhibiting UV-resistance. Correspondingly, only in one of three patients exhibiting UV-sensitivity was the difference in DNA migration statistically significant (maximum increase: 44-107 microm, vs. 24 h after: 42-100 microm). Our results confirm an abnormal pattern of the CLL cell response to UV irradiation. Furthermore, we identified defective processing of UV-induced DNA damage in CLL versus normal lymphocytes, particularly in UV-sensitive cases.
Myllyperkiö
M H
MH
Laboratory of Molecular Hematology, Department of Clinical Chemistry, Tampere University Hospital, P.O. Box 2000, 33521, Tampere, Finland.
Koski
T R
TR
Vilpo
L M
LM
Vilpo
J A
JA
D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000528:radiation effects; D020552:Comet Assay / Q000379:methods; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000528:radiation effects; D005260:Female; D006801:Humans; D007700:Kinetics; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000532:radiotherapy*; D007963:Leukocytes, Mononuclear / Q000528:radiation effects; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D008875:Middle Aged; D011836:Radiation Tolerance; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded