Midkine enhances early stages of collagen gel contraction.
Midkine (MK) is a heparin-binding growth/differentiation factor implicated in the control of development and repair of various tissues. To investigate the roles of MK in embryogenesis and in regulation of wound healing, we utilized a system of collagen gel contraction by dermal fibroblasts, which provides an in vitro model for studying fibroblast-collagen interactions important in various physiological and pathological phenomena. MK enhanced gel contraction 8-24 h after plating, and its effect was inhibited by anti-MK antibody. The effect was reduced after 48 h, while TGF-beta continued to be active in the later stage. Morphologically, MK-treated fibroblasts tended to be elongated more frequently than control fibroblasts. The effect of MK in the early stage of gel contraction suggests a role of MK as a modulator of cell-matrix interactions.
Sumi
Y
Y
Department of Biochemistry and Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
Muramatsu
H
H
Hata
K
K
Ueda
M
M
Muramatsu
T
T
D002352:Carrier Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000187:drug effects*; D016207:Cytokines; D005347:Fibroblasts / Q000187:drug effects*; D005782:Gels / Q000737:chemistry; D006801:Humans; D000078792:Midkine; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000378:metabolism
D002352:Carrier Proteins; D016207:Cytokines; D005782:Gels; D016212:Transforming Growth Factor beta; D000078792:Midkine; D003094:Collagen