title: The phage infection process: a functional role for the distal linker region of bacteriophage protein 3.
abstract: The filamentous bacteriophage infects Escherichia coli by interaction with the F pilus and the TolQRA complex. The virus-encoded protein initiating this process is the gene 3 protein (g3p). The g3p molecule can be divided into three different domains separated by two glycine-rich linker regions. Though there has been extensive evaluation of the importance of the diverse domains of g3p, no proper function has so far been assigned to these linker regions. Through the design of mutated variants of g3p that were displayed on the surface of bacteriophage, we were able to elucidate a possible role for the distal glycine-rich linker region. A phage that displayed a g3p comprised of only the N1 domain, the first linker region, and the C-terminal domain was able to infect cells at almost the same frequency as the wild-type phage. This infection was proven to be dependent on the motif between amino acid residues 68 and 86 (i.e., the first glycine-rich linker region of g3p) and on F-pilus expression.
authors.affiliation: Department of Immunotechnology, Lund University, S-220 07 Lund, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Nilsson
authors.forename: A C
authors.initials: AC
authors.lastname: Malmborg
authors.forename: C A
authors.initials: CA
authors.lastname: Borrebaeck
mesh_terms: D001665:Binding Sites; D002122:Calcium Chloride / Q000494:pharmacology; D036022:Capsid Proteins; D003090:Coliphages / Q000187:drug effects / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000821:virology*; D005998:Glycine / Q000378:metabolism; D018933:Pili, Sex / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D004268:DNA-Binding Proteins; D014760:Viral Fusion Proteins; D002122:Calcium Chloride; D005998:Glycine