title: Assays for activation of recombinant expressed opsins by all-trans-retinals.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA.
authors.forename: M
authors.initials: M
authors.lastname: Han
authors.forename: T P
authors.initials: TP
authors.lastname: Sakmar
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012172:Retinaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*; D017299:Rod Opsins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D017299:Rod Opsins; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D012243:Rhodopsin; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D012172:Retinaldehyde