[Identification of herba taraxaci and its adulterants in Hong Kong market by DNA fingerprinting with random primed PCR].
DNA fingerprinting for Taraxacum mongolicum and its adulterants of six species of Compositae was demonstrated with random-primed polymerase chain reaction (PCR) including arbitrarily-primed PCR (AP-PCR) and random-amplified polymorphic DNA(RAPD). Distinctive genomic fingerprints from DNA of Taraxacum mongolicum and its adulterants were generated with two long (20-24 mer) and one short (10 mer) random-chosen primers Taraxacum mongolicum can be distinguished from six adulterants according to the banding patterns of their amplified DNA on agarose gels. Moreover, that Taraxacum mongolicum and its adulterant are unrelated. The Similarity Indexes values of the genomic fingerprints. Results showed AP-PCR and RAPD might be used for identifying Chinese drugs.
Cao
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Department of Biology, Chinese University of Hong Kong.
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PP
Shaw
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D019659:Asteraceae / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics*; D004340:Drug Contamination; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant