Th2 immune regulation induced by T cell vaccination in patients with multiple sclerosis.
T cell responses to myelin basic protein (MBP) are potentially involved in the pathogenesis of multiple sclerosis (MS). In this study, we demonstrated that subcutaneous inoculations with irradiated autologous MBP-reactive T cell clones (T cell vaccination) elicited CD8+ anti-idiotypic T cell responses and CD4+ Th2 cell responses in patients with MS. Both regulatory cell types induced by T cell vaccination contributed to the inhibition of MBP-reactive T cells while they differed in the recognition pattern and functional properties. We describe for the first time that the Th2 regulatory cells reacted with activated but not resting T cells in the context of MHC class II molecules and inhibited the proliferation of MBP-reactive T cells through the secretion of IL-4 and IL-10. The T-T cell interaction mediated by Th2 regulatory cells was independent of the antigen specificity of activated T cells. The findings have important implications for our understanding of the regulatory mechanism induced by T cell vaccination.
Zang
Y C
YC
Multiple Sclerosis Research Laboratory, Department of Neurology, Baylor College of Medicine, Houston 77030, USA. jzang@bcm.tmc.edu
Hong
J
J
Tejada-Simon
M V
MV
Li
S
S
Rivera
V M
VM
Killian
J M
JM
Zhang
J Z
JZ
D015551:Autoimmunity; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation; D009103:Multiple Sclerosis / Q000276:immunology* / Q000628:therapy*; D004676:Myelin Basic Protein / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014611:Vaccination
D016207:Cytokines; D004676:Myelin Basic Protein