Isolation of a novel PDZ-containing myosin from hematopoietic supportive bone marrow stromal cell lines.
Stromal cells in bone marrow provide an optimal microenvironment for hematopoiesis. The established stromal cell lines from bone marrow showed various cellular heterogeneities and differed in their hematopoietic supportive ability. By a differential display method, we cloned a gene whose expression levels were correlated with the hematopoietic supportive ability of stromal cells. Its deduced amino acid sequence shows a structure similar to myosins, except that it lacks an actin binding site. Interestingly, it contains a KE-rich sequence and a PDZ domain in the NH(2)-terminal, which are protein-protein interaction domains; therefore we termed this novel myosin Myosin containing PDZ domain (MysPDZ). Western blot analysis showed that its protein levels positively correlated with the supportive ability of stromal cells and immunostaining suggested that MysPDZ was present at cytoskeleton in a filamentous and/or network form. Thus MysPDZ may be involved in the maintenance of the stromal cell architectures required for cell to cell contact.
Furusawa
T
T
Department of Cell Biology, Institute of Development, Aging, and Cancer, Tohoku University, Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
Ikawa
S
S
Yanai
N
N
Obinata
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000737:chemistry; D019143:Evolution, Molecular; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D020409:Molecular Motor Proteins; D008969:Molecular Sequence Data; D009218:Myosins / Q000235:genetics / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D017154:Stromal Cells / Q000737:chemistry* / Q000166:cytology
D020409:Molecular Motor Proteins; C407101:Myo18a protein, mouse; D009218:Myosins