Case-control study in a subtropical Australian population to assess the relation between non-melanoma skin cancer and epidermodysplasia verruciformis human papillomavirus DNA in plucked eyebrow hairs. The Nambour Skin Cancer Prevention Study Group.
Epidermodysplasia verruciformis human papillomavirus (EV-HPV) DNA has been demonstrated in malignant and benign skin lesions and in hairs plucked from renal transplant recipients and immunocompetent patients. We investigated the association between EV-HPV DNA in hairs plucked from eyebrows and the occurrence of non-melanoma skin cancer (NMSC) in a community-based study. Within a cohort of residents of a Queensland township (Nambour), nested case-control studies of recently developed NMSC (64 cases), basal-cell carcinoma (BCC) (51 cases) and squamous-cell carcinoma (SCC) (25 cases) were conducted. EV-HPV DNA in hair and a small number of available tumour samples was detected using a nested PCR specific for EV-HPV types. EV-HPV DNA was detected in hairs from 94 of 143 individuals (66%), and 36 (39%) of the samples contained 2 or more different EV-HPV types. Only known or putatively new EV-HPV types were detectable after sequencing 93 samples. EV-HPV status agreed for 12 of 20 subjects who had both hair and skin tumour samples available. In 4 of 5 pairs of positive samples, the same EV-HPV type was found. There were non-significant negative associations between EV-HPV and NMSC (OR 0.77, 95% CI 0.34-1.8) and BCC (OR 0.58, 95% CI 0.23-1.5) but a non-significant positive association with SCC (OR 2.00, 95% CI 0.50-8.0).
Boxman
I L
IL
Department of Virology, Academic Medical Centre, Amsterdam, The Netherlands. i.l.boxman@amc.uva.nl
Russell
A
A
Mulder
L H
LH
Bavinck
J N
JN
Schegget
J T
JT
Green
A
A
D000328:Adult; D000368:Aged; D001315:Australia / Q000453:epidemiology; D002280:Basal Cell Carcinoma / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000821:virology*; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000821:virology*; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis*; D004819:Epidermodysplasia Verruciformis / Q000235:genetics / Q000276:immunology / Q000821:virology*; D005138:Eyebrows / Q000821:virology*; D005260:Female; D006801:Humans; D007121:Immunocompetence; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000821:virology; D015995:Prevalence; D012878:Skin Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000821:virology*; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000821:virology*
D004279:DNA, Viral