Polyomavirus large T antigen mutants affected in viral DNA replication.
We have characterized two polyomavirus large T antigen mutants with different properties in viral DNA replication. dl-97, a mutant active in immortalization, exerts a dominant negative effect in viral DNA replication. 13val, which is defective in both immortalization and viral DNA replication, has a lesion in the putative DnaJ domain affecting the block of Rb function.
Lemieux
B
B
Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada.
Bastin
M
M
D016475:3T3 Cells; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000502:physiology*; D002460:Cell Line; D004261:DNA Replication; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D051381:Rats; D014162:Transfection; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming