A simple approach for the simultaneous isolation and immobilization of invertase using crude extracts of yeast and Jack bean meal.
Crude cell-free extract of yeast cells was mixed with sufficient amount of Jack bean meal extract so as to precipitate all the invertase. The precipitate was then cross-linked using 2% glutaraldehyde retaining over 60% of the activity. The immobilized invertase could be reused for over ten batches without loss in activity.
Melo
J S
JS
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India.
D'Souza
S F
SF
D002474:Cell-Free System; D003208:Concanavalin A; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D007887:Fabaceae / Q000201:enzymology; D005433:Flour; D005976:Glutaral; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D037121:Plant Lectins; D010946:Plants, Medicinal; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D043324:beta-Fructofuranosidase
D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D037121:Plant Lectins; D003208:Concanavalin A; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005976:Glutaral