Promotion of vesicular stomatitis virus nucleocapsid protein production by arthopod saliva.
In a previous study (Hajnicka, V. et al., Parasitology 116, 533-538, 1998), the infectivity titer of vesicular stomatitis virus (VSV) was shown to increase up to 10,000-fold when mouse L cells were treated with tick salivary gland extract (SGE) prior to infection. To examine this effect at the level of viral protein production, radiolabeled VSV-infected cells were analyzed by double-dimensional gel electrophoresis. A pre-treatment of cells with SGE from partially fed ticks in amounts corresponding to 1 or 3 salivary glands increased the level of both viral nucleocapsid (N) protein and phosphoprotein (P) in a dose-dependent manner. The effect was more pronounced for N protein and could account for the dramatic increase in infectious virus yield. Promotion of viral infectivity by arthropod saliva may support the arthropode-borne transmission cycle of VSV.
Kocáková
P
P
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Hajnická
V
V
Slovák
M
M
Nuttall
P A
PA
Fuchsberger
N
N
D000818:Animals; D001096:Arachnid Vectors / Q000502:physiology; D002460:Cell Line; D003870:Dermacentor / Q000502:physiology*; D051379:Mice; D019251:Nucleocapsid / Q000096:biosynthesis*; D019590:Nucleocapsid Proteins; D010750:Phosphoproteins; D012463:Saliva; D012469:Salivary Glands / Q000737:chemistry / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism* / Q000502:physiology; D015678:Viral Structural Proteins / Q000378:metabolism; D014779:Virus Replication
D019590:Nucleocapsid Proteins; C081250:P protein, Vesicular stomatitis virus; D010750:Phosphoproteins; D015678:Viral Structural Proteins