title: Ca2+-bound calmodulin forms a compact globular structure on binding four trifluoperazine molecules in solution.
abstract: Small-angle X-ray scattering (SAXS), which determines the radius of gyration, R(g), and the pair distance distribution function, was used to investigate the conformational changes of calmodulin (CaM) on binding to an antagonist, trifluoperazine (TFP), with or without Ca(2+) in solution. We previously applied this SAXS method to CaM complexed with N-(6-aminohexyl)-5-chloro-1-naphthalenesulphonamide (W-7) [Osawa, Kuwamoto, Izumi, Yap, Ikura, Shibanuma, Yokokura, Hidaka and Matsushima (1999) FEBS Lett. 442, 173-177] and found that the binding of two W-7 TFP molecules to one Ca(2+)-saturated CaM molecule induces structural changes from a 'dumb-bell' shape to a compact globular shape. We report here that the most compact globular shape whose size is consistent with that of the 1:2 Ca(2+)-saturated CaM-W-7 complex is formed by the binding of four TFP molecules to one Ca(2+)-saturated CaM molecule. Even in the absence of Ca(2+), the conformational changes of CaM occur on TFP binding, giving a slightly smaller R(g) than Ca(2+)-free CaM alone.
authors.affiliation: Department of Biophysics, School of Health Sciences, Sapporo Medical University, Sapporo, Hokkaido 060-8556, Japan. matusima@shs.sapmed.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Matsushima
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Jinbo
authors.forename: Y
authors.initials: Y
authors.lastname: Izumi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012996:Solutions; D013449:Sulfonamides / Q000493:pharmacokinetics; D014268:Trifluoperazine / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D002147:Calmodulin; D011994:Recombinant Proteins; D012996:Solutions; D013449:Sulfonamides; D014268:Trifluoperazine; C017967:W 7; D002118:Calcium