Difference in hydrophobicity between botulinum type B activated and non-activated neurotoxins under low pH conditions.
We showed that botulinum type B activated neurotoxin with a di-chain structure became hydrophobic more quickly and extensively than did the non-activated toxin with a single-chain structure on low pH exposure. The activated toxin possessed 50-fold higher toxicity than did the non-activated type. The difference in the susceptibility to hydrophobic change may be one clue to answering the question of why the activated toxin possesses a higher toxicity than does the non-activated type.
Kamata
Y
Y
Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka, Japan. kamata@riast.osakafu-u.ac.jp
Tahara
R
R
Kozaki
S
S
D001905:Botulinum Toxins / Q000737:chemistry* / Q000633:toxicity; D019274:Botulinum Toxins, Type A; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002848:Chromatography, DEAE-Cellulose; D005456:Fluorescent Dyes; D005732:Gangliosides / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009282:Naphthalenesulfonates; D009498:Neurotoxins / Q000737:chemistry* / Q000633:toxicity; D010743:Phospholipids / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D005732:Gangliosides; D009282:Naphthalenesulfonates; D009498:Neurotoxins; D010743:Phospholipids; C096323:rimabotulinumtoxinB; C009497:2-(4-toluidino)-6-naphthalenesulfonic acid; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A