Crystallization of engineered Thermus flavus 5S rRNA under earth and microgravity conditions.
Thermus flavus 5S rRNA with a molecular weight of about 40 kDa was modified at the 5' and 3' ends. Crystals were obtained under earth and microgravity conditions. The best crystals were obtained during NASA space mission STS 94. For the first time, it was possible to collect a complete data set from 5S rRNA crystals to 7.8 A resolution and to assign the space group as R32, with unit-cell parameters a = b = 110.3, c = 387.6 A, alpha = beta = 90, gamma = 120 degrees.
Lorenz
S
S
Institut für Biochemie, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany.
Perbandt
M
M
Lippmann
C
C
Moore
K
K
DeLucas
L J
LJ
Betzel
C
C
Erdmann
V A
VA
D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D005818:Genetic Engineering; D006112:Gravitation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012341:RNA, Ribosomal, 5S / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013824:Thermus / Q000737:chemistry* / Q000235:genetics; D014893:Weightlessness
D012329:RNA, Bacterial; D012341:RNA, Ribosomal, 5S