Cell adhesion inhibition by glycoliposomes: effects of vesicle diameter and ligand density.
The Thomsen-Friedenreich antigen (TF) is a pancarcinoma marker which is involved in the development of liver metastasis by binding tumour cells to the asialoglycoprotein receptor on hepatocytes. Blocking of this receptor prevents metastasis under certain circumstances. We report on conditions for an effective inhibition of the adhesion of KG-1 leukaemia cells expressing TF by lactosylated liposomes. In order to reach strong inhibition, carbohydrate blocking probes must be multivalent. Glycoliposomes are able to carry a large number of glycolipids accommodated in the lipid bilayer. They should be able to adapt their glycolipid pattern in order to achieve multiple binding. We found that, in addition to the number of carbohydrates on the liposome surface, their size, and probably the arrangement of neutral glycolipids in clustered domains, determine the inhibitory properties of glycoliposomes.
Stahn
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Max Delbrück Center for Molecular Medicine, Berlin, Germany. rstahn@mdc-berlin.de
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D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D005690:Galactose / Q000378:metabolism; D006017:Glycolipids / Q000032:analysis*; D006031:Glycosylation; D006801:Humans; D020128:Inhibitory Concentration 50; D007785:Lactose / Q000378:metabolism; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D008024:Ligands; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008954:Models, Biological; D009362:Neoplasm Metastasis / Q000473:pathology; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D012276:Ricin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D015295:Antigens, Tumor-Associated, Carbohydrate; D006017:Glycolipids; D008024:Ligands; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C016467:Thomsen-Friedenreich antigen; D012276:Ricin; D007785:Lactose; D005690:Galactose