A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NoIL in Rhizobium leguminosarum.
Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).
Pacios Bras
C
C
Leiden University, Institute of Molecular Plant Sciences, The Netherlands.
Jordá
M A
MA
Wijfjes
A H
AH
Harteveld
M
M
Stuurman
N
N
Thomas-Oates
J E
JE
Spaink
H P
HP
D020561:Alphaproteobacteria / Q000235:genetics; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000302:isolation & purification; D001426:Bacterial Proteins; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000302:isolation & purification; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000382:microbiology*; D016961:Rhizobium leguminosarum / Q000235:genetics / Q000378:metabolism*; D013559:Symbiosis / Q000235:genetics* / Q000502:physiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D010940:Plant Proteins; C023023:lipid-linked oligosaccharides; C117779:noIL protein, Rhizobium etli; D005647:Fucosyltransferases; C103941:NodZ protein, bacteria