Survival and growth of Burkholderia cepacia within the free-living amoeba Acanthamoeba polyphaga.
The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia.
Landers
P
P
Department of Microbiology, University of Leeds, UK.
Kerr
K G
KG
Rowbotham
T J
TJ
Tipper
J L
JL
Keig
P M
PM
Ingham
E
E
Denton
M
M
D000048:Acanthamoeba / Q000254:growth & development / Q000382:microbiology*; D000818:Animals; D016956:Burkholderia cepacia / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D006801:Humans
D003470:Culture Media