Gene-trap-based target site for cre-mediated transgenic insertion.
There is an increasing need for tissue-specific gene expression regulatory elements to study normal and disease development in the mouse. However, the cloning and characterization of these elements are time-consuming and costly. Thus, there is a particular need to be able to identify gene expression patterns without having to clone the promoter elements. Gene-trap strategies identify expression patterns assigned for endogenous genes using reporters, such as LacZ (Gossler et al., 1989; Skarnes, 1990) or green fluorescent protein (GFP) (Ishida and Leder, 1999; Zheng and Hughes, 1999). The gene-trap vector randomly inserts into the genome and "steals" regulatory elements for the reporter. Here we describe an improved gene-trap strategy, which allows an efficient Cre recombinase-mediated insertion of any transgene into the trapped loci as a post-integrational modification and links the expression of the transgene to that of the reporter.
Hardouin
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Nagy
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D000818:Animals; D002460:Cell Line; D020869:Gene Expression Profiling; D018390:Gene Targeting; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D019426:Integrases / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D014764:Viral Proteins
D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases