title: Relationships between natural T cells, atopy, IgE levels, and IL-4 production.
abstract: BACKGROUND
  Th2 cells govern allergic disorders. Mechanisms leading to the Th2 commitment are dominated by the requirement of IL-4. A potential source of this triggering IL-4 could be the CD4 + subset of a small population of T cells, natural T (NT) cells. Indeed, this subset is involved in IgE responses in mice and produces promptly high amounts of IL-4 in both mice and man.
  
  
  METHODS
  NT cells were identified in peripheral blood by flow cytometry with antibodies against Valpha24 and Vbeta11, recognizing the T-cell receptor specific for NT cells. Simultaneous staining with anti-CD3, anti-CD4, or anti-CD8 antibodies was performed. The frequency of NT cells in man was studied according to the presence of atopy defined by the positivity of skin tests, according to total IgE levels in serum, and according to IL-4 concentration of whole-blood culture supernatants determined by a flow cytometer microsphere-based assay.
  
  
  RESULTS
  Seventy subjects were included, of whom 30 were atopic. The number of CD4+ NT cells was higher in atopics than in nonatopics (P=0.009). This number was correlated to the total IgE levels (r = 0.34, P = 0.03). In addition, the number of CD4 + NT cells, but also of CD8 + NT cells, was correlated to the levels of IL-4 (r=0.71, P=0.01, and r=0.6, P=0.03, respectively).
  
  
  CONCLUSIONS
  These results show that the number of NT cells, particularly the CD4+ subset, is related to atopy, IL-4 production, and IgE levels. Therefore, this population of T cells is likely to play a role in the Th2 commitment initiating atopic diseases.
authors.affiliation: UPRES 2050, Service de Pneumo-Allergologie, INSERM, Assistance Publique Hôpitaux de Marseille, Hopital Ste Marguerite, France.
authors.forename: A
authors.initials: A
authors.lastname: Magnan
authors.forename: L
authors.initials: L
authors.lastname: Mély
authors.forename: S
authors.initials: S
authors.lastname: Prato
authors.forename: D
authors.initials: D
authors.lastname: Vervloet
authors.forename: F
authors.initials: F
authors.lastname: Romagné
authors.forename: C
authors.initials: C
authors.lastname: Camilla
authors.forename: A
authors.initials: A
authors.lastname: Necker
authors.forename: B
authors.initials: B
authors.lastname: Casano
authors.forename: F
authors.initials: F
authors.lastname: Montero-Jullian
authors.forename: V
authors.initials: V
authors.lastname: Fert
authors.forename: B
authors.initials: B
authors.lastname: Malissen
authors.forename: P
authors.initials: P
authors.lastname: Bongrand
mesh_terms: D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018929:Cell Culture Techniques; D005434:Flow Cytometry; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis*; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000096:biosynthesis*; D007428:Intradermal Tests; D018655:Lymphocyte Count; D008875:Middle Aged; D018418:Th2 Cells / Q000276:immunology
chemical_list: D015847:Interleukin-4; D007073:Immunoglobulin E