Kinetic evidence of an on-pathway intermediate in the folding of lysozyme.
By means of a kinetic test, it was demonstrated that one of the folding intermediates (Ialpha) of hen lysozyme with alpha-domain folded and beta-domain unfolded is on the folding pathway under the classical definition. Ialpha folds to the native (N) state directly (unfolded (U) <==> Ialpha <==> N) without having to unfold to U and then refold to N through alternative folding pathways as in Ialpha <==> U <==> N.
Bai
Y
Y
Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20850, USA. yawen@helix.nih.gov
D000818:Animals; D002645:Chickens; D004529:Egg White; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry*; D017510:Protein Folding
D009113:Muramidase