title: [Restriction endonucleases from various bacterial strains exhibit an ice-nucleating activity].
abstract: Six strains containing site-specific endonucleases II were selected from a collection of 45 ice-nucleating bacterial strains isolated from rhizosphere of plants growing in various geographical regions. Endonucleases Pfl211I, Psp8I, and Psp23I were isolated and purified from two Pseudomonas sp. strains and a Pseudomonas fluorescens strain. Restriction endonucleases Pfl21I and Psp23I were shown to recognize and cleave the DNA nucleotide sequence 5'-CTGCA decrease G-3'. Endonuclease Psp8I recognized and cleaved the DNA nucleotide sequence 5'-G decrease GATCC-3'. These endonucleases were found to be true isoschizomers of PstI and BamHI, respectively.
authors.affiliation: Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia. pertsev@ibpm.serpukhov.su
authors.forename: A V
authors.initials: AV
authors.lastname: Pertsev
authors.forename: M M
authors.initials: MM
authors.lastname: Den'mukhametov
authors.forename: A G
authors.initials: AG
authors.lastname: Anoshkin
authors.forename: E V
authors.initials: EV
authors.lastname: Ariskina
authors.forename: I A
authors.initials: IA
authors.lastname: Berezin
authors.forename: A S
authors.initials: AS
authors.lastname: Solonin
authors.forename: N P
authors.initials: NP
authors.lastname: Kuz'min
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D007053:Ice; D011549:Pseudomonas / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D007053:Ice; D015252:Deoxyribonucleases, Type II Site-Specific