Comparative in vitro isolation of Trypanosoma theileri from cattle in Belgium.
Ten blood samples randomly collected from cows on a farm nearby Antwerp, Belgium, were inoculated into KIVI culture medium (Kit for In Vitro Isolation of trypanosomes) and RPMI 10%+feeder medium. Within 3 weeks of incubation all KIVI cultures and four RPMI 10%+feeder revealed presence of Trypanosoma theileri. Some practical implications regarding the use of KIVI for isolation of pathogenic African trypanosomes from cattle and other Bovidae are discussed.
Verloo
D
D
Department of Parasitology, Institute of Tropical Medicine, Nationalestraat 155, B-2000, Antwerp, Belgium. dverloo@itg.be
Brandt
J
J
Van Meirvenne
N
N
Büscher
P
P
D000818:Animals; D001530:Belgium; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D003470:Culture Media; D014345:Trypanosoma / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000469:parasitology / Q000662:veterinary*
D003470:Culture Media