title: The eEFIA gene family is differentially expressed in maize endosperm.
abstract: eEF1A appears to be a multifunctional protein in eukaryotes, where it serves as a protein synthesis factor as well as a cytoskeletal protein. In maize endosperm, the eEF1A concentration is highly correlated with lysine content, and eEF1A synthesis is increased in opaque2 mutants compared to wild type. To investigate the basis for the increased synthesis of eEF1A in opaque2, we characterized the genes encoding this protein and measured their relative level of expression in endosperm and other tissues. Maize contains 10 to 15 eEF1A genes that are nearly identical in nucleotide and amino acid sequences. However, these genes can be distinguished based on their 3' non-coding sequences, which are less conserved. By screening endosperm and seedling cDNA libraries, we show that most of the maize eEF1A genes are expressed, and the relative level of their transcripts varies in different tissues. At least five genes are transcribed in the endosperm, and two account for ca. 80% of the RNA transcripts. The expression of several genes is enhanced in opaque2 endosperm, although the significance of this is unclear.
authors.affiliation: Department of Plant Sciences, University of Arizona, Tucson 85721, USA.
authors.forename: N P
authors.initials: NP
authors.lastname: Carneiro
authors.forename: P A
authors.initials: PA
authors.lastname: Hughes
authors.forename: B A
authors.initials: BA
authors.lastname: Larkins
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018628:Gene Dosage; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010944:Plants / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D020648:Peptide Elongation Factor 1; D018749:RNA, Plant