Toxicity of cinnamomin--a new type II ribosome-inactivating protein to bollworm and mosquito.
The toxicity of cinnamomin, a new type II ribosome-inactivating protein purified from the seeds of camphor tree (Cinnamomum camphora), to bollworm (Helicoverpa armigera) and mosquito (Culex pipines pallens) during larval stage was tested. The LC50 of cinnamomin to bollworm larvae fed on diet containing cinnamomin was 1839 ppm and the LC50 to larvae of mosquito was 168 ppm. The gut extract of bollworm larvae could apparently hydrolyze cinnamomin. The inhibition of protein synthesis by cinnamomin was tested in in vitro translation system of bollworm larvae, and its LC50 was determined to be approx. 14 nM. Bollworm larvae ribosome treated with cinnamomin produced a specific RNA fragment (R-fragment) characterized on urea-denatured polyacrylamide gel. Evidence was provided that hidden breaks exist in the largest ribosomal RNA of bollworm larvae.
Zhou
X
X
Shanghai Institute of Biochemistry, Academia Sinica, China.
Li
X D
XD
Yuan
J Z
JZ
Tang
Z H
ZH
Liu
W Y
WY
D020418:Algal Proteins; D000818:Animals; D002474:Cell-Free System; D003465:Culex; D007300:Insect Control / Q000379:methods; D007306:Insecticides; D007814:Larva; D009036:Moths; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D010940:Plant Proteins; D011506:Proteins; D054788:Ribosome Inactivating Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes
D020418:Algal Proteins; D007306:Insecticides; D010940:Plant Proteins; D011506:Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C061930:cinnamomin, Phytophthora cinnamomi; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins