Lipid oxidation deletes the nanodomain organization of artificial membranes.
Nanoscopic domains with different crystal structures have been induced in closed artificial membranes and have been directly imaged by atomic force microscopy at a spatial resolution better than 0.3 nm. These observations provide experimental evidence to the hydrophobic mismatching theory of lateral phase separation phenomena. Under oxidant conditions, the lipid-lipid assembly reorganizes into a new steady-state structure with disappearance of specific nanodomains. This finding may contribute to understanding the mechanism of peroxidative damage to membrane properties. In fact, alterations of specific modes of molecular conformation and packing may lead to perturbation of specific properties.
Muscatello
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U
Department of Biomedical Sciences, University of Modena, Italy. muscatel@unimo.it
Alessandrini
A
A
Valdré
G
G
Vannini
V
V
Valdré
U
U
D008055:Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D018625:Microscopy, Atomic Force; D010084:Oxidation-Reduction; D014961:X-Ray Diffraction
D008055:Lipids; D008567:Membranes, Artificial