title: [The use of in situ polymerase chain reaction for detection the intracellular localization of Mycoplasma hominis in cultured HeLa cells].
abstract: Mycoplasma hominis was incubated with HeLa cells up to 72 h. Infection of HeLa cells was estimated by in situ polymerase chain reaction with a subsequent use of computer digital image analysis for result processing. The localization of mycoplasma in the cell nucleus was demonstrated after a 48 h incubation. The quantity of infected HeLa cells was increased with time to make 58 and 75% on days 2 and 3, respectively. No M. hominis was found in the cytoplasma of HeLa cells.
authors.affiliation: Research Institute of Physico-Chemical Medicine, Ministry of Public Health, Russian Federation, Moscow.
authors.forename: K T
authors.initials: KT
authors.lastname: Momynaliev
authors.forename: V F
authors.initials: VF
authors.lastname: Orlova
authors.forename: A V
authors.initials: AV
authors.lastname: Zhukotskiĭ
authors.forename: E M
authors.initials: EM
authors.lastname: Kogan
authors.forename: V M
authors.initials: VM
authors.lastname: Govorun
mesh_terms: D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D019535:Mycoplasma hominis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity