title: Sequence of the gene encoding hsp90e from Cryptosporidium parvum.
abstract: A composite 2364 nt DNA sequence with an open reading frame (ORF) encoding an endoplasmic reticulum-associated heat shock protein 90 (CpHsp90e) was determined from clones isolated from genomic libraries constructed from the KSU-1 isolate of Cryptosporidium parvum. Transcription was verified by isolation of a clone from a cDNA library with a similar restriction map to that observed with genomic DNA. The predicted protein consists of 787 amino acids, has a predicted molecular size of 89.2 kDa, and was found to share strong homology with other endoplasmic reticulum-associated hsp90 proteins.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506, USA. kemew@ksu.edu
authors.forename: K M
authors.initials: KM
authors.lastname: Woods
authors.forename: M
authors.initials: M
authors.lastname: Tilley
authors.forename: A
authors.initials: A
authors.lastname: Iseli
authors.forename: S J
authors.initials: SJ
authors.lastname: Upton
authors.forename: B A
authors.initials: BA
authors.lastname: Montelone
authors.forename: N V
authors.initials: NV
authors.lastname: Khramtsov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016785:Cryptosporidium parvum / Q000235:genetics*; D016054:DNA, Protozoan; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid
chemical_list: D016054:DNA, Protozoan; D018841:HSP90 Heat-Shock Proteins