title: Quantitative multi-modal functional MRI with blood oxygenation level dependent exponential decays adjusted for flow attenuated inversion recovery (BOLDED AFFAIR).
abstract: A magnetic resonance imaging (MRI) method is described that allows interleaved measurements of transverse (R(2)(*) and R(2)) and longitudinal (R(1)) relaxation rates of tissue water in conjunction with spin labeling. The image-contrasts are intrinsically blood oxygenation level dependent (BOLD) and cerebral blood flow (CBF) weighted, but each contrast is made quantitative by two echo time (TE) and inversion recovery time (TIR) acquisitions with gradient echo (GE) and spin echo (SE) weighted echo-planar imaging (EPI). The EPI data were acquired at 7 Tesla with nominal spatial resolution of 430 x 430 x 1000 microm(3) in rat brain in vivo. The method is termed as blood oxygenation level dependent exponential decays adjusted for flow attenuated inversion recovery (BOLDED AFFAIR) and allows acquisition of R(2)(*), R(2), and CBF maps in an interleaved manner within approximately 12 minute. The basic theory of the method, associated experimental/systematic errors, and temporal restrictions are discussed. The method is validated by comparison of multi-modal maps obtained by BOLDED AFFAIR (i.e., two TE and TIR values with GE and SE sequences) and conventional approach (i.e., multiple TE and TIR values with GE and SE sequences) during varied levels of whole brain activity. Preliminary functional data from a rat forepaw stimulation model demonstrate the feasibility of this method for functional MRI (fMRI) studies. It is expected that with appropriate precautions this method in conjunction with contrast agent-based MRI has great potential for quantitative fMRI studies of mammalian cortex.
authors.affiliation: Department of Diagnostic Radiology, Magnetic Resonance Center, PO Box 208043, Yale University, New Haven, CT 06510, USA. fahmeed.hyder@yale.edu
authors.forename: F
authors.initials: F
authors.lastname: Hyder
authors.forename: R
authors.initials: R
authors.lastname: Renken
authors.forename: R P
authors.initials: RP
authors.lastname: Kennan
authors.forename: D L
authors.initials: DL
authors.lastname: Rothman
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D001834:Body Water / Q000378:metabolism; D001921:Brain / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001931:Brain Mapping / Q000295:instrumentation / Q000379:methods*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D017352:Echo-Planar Imaging / Q000379:methods*; D004558:Electric Stimulation; D005069:Evaluation Studies as Topic; D005552:Forelimb; D007091:Image Processing, Computer-Assisted; D008297:Male; D008962:Models, Theoretical; D010100:Oxygen / Q000097:blood*; D010424:Pentobarbital / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013113:Spin Labels
chemical_list: D013113:Spin Labels; D010424:Pentobarbital; D010100:Oxygen