[Constructing of system of genetic analysis in Pseudomonas mendocina].
A Tn10-containing variant of the pRK2013 plasmid, pRK2013-7, was used in the genetic analysis of Pseudomonas mendocina as chromosome-mobilizing inheritable factor that is able to integrate into the bacterial chromosome and transfer genetic markers with a frequency ranging from 3.2 x 10(-7) to 3.5 x 10(-3). The results of interrupted matings allowed localization of 10 genetic markers. This system of genetic analysis is suitable for P. mendocina mapping.
Vasilenko
S L
SL
Belorussian State University, Department of Genetics and Biotechnology, Minsk, Belarus.
Titok
M A
MA
Maksimova
N P
NP
D002874:Chromosome Mapping / Q000379:methods*; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D011549:Pseudomonas / Q000235:genetics*