Genetic diversity of the Campylobacter genes coding immunodominant proteins.
Three Campylobacter jejuni 72Dz/92 genes (cjaA (ompH1), cjaC (hisJ) and cjaD (omp18)) encoding immunodominant proteins are considered to be potential chicken vaccine candidates. The presence and conservation of cjaA, cjaC and cjaD genes among different Campylobacter clinical isolates were determined. The genes were detected in thirty Campylobacter strains using hybridization as well as Western blot analysis. However, PCR products of the predicted size were amplified only from ten out of thirty examined strains regardless of the employed primer pair. The nucleotide sequence of the C. jejuni 72Dz/92 genes was compared with the nucleotide sequences of their homologs cloned from other Campylobacter strains as well as with the whole genome sequence of C. jejuni NCTC 11168. The examined sequences revealed 0 to 16% divergence. Strain-dependent levels of divergence were observed. The polymorphism detected in cjaC was mainly within the 5' region of the gene, while the nucleotide substitutions in cjaA and cjaD are distributed uniformly along the whole genes. Most of the observed nucleotide substitutions occurred at the third base of the codons. This observation is consistent with the results of Western blot experiments.
Pawelec
D P
DP
Institute of Microbiology, Warsaw University, Nowy OEwiat 67, 00-046, Warsaw, Poland.
Korsak
D
D
Wyszyńska
A K
AK
Rozynek
E
E
Popowski
J
J
Jagusztyn-Krynicka
E K
EK
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins; D001483:Base Sequence; D002167:Campylobacter / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000382:microbiology*; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D002648:Child; D017124:Conserved Sequence; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000235:genetics*; D007231:Infant, Newborn; D008074:Lipoproteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods
D018528:ATP-Binding Cassette Transporters; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C111021:CjaC protein, Campylobacter jejuni; D004269:DNA, Bacterial; D016056:Immunodominant Epitopes; D008074:Lipoproteins