Identification of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, arcobacter butzleri, and A. butzleri-like species based on the glyA gene.
Currently, the detection and identification of Campylobacter and Arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. We have developed a PCR-hybridization strategy, wherein degenerate primers are used to amplify glyA fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, Arcobacter butzleri, and an A. butzleri-like species. Evaluation of this strategy with genomic DNA from different type strains suggests that this approach is both specific and sensitive and thus may be applicable in a diagnostic assay to identify and differentiate these highly related species.
Al Rashid
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Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada M5S 1A8.
Dakuna
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I
Louie
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Ng
D
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Vandamme
P
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Johnson
W
W
Chan
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VL
D020640:Arcobacter / Q000145:classification* / Q000201:enzymology; D001483:Base Sequence; D015139:Blotting, Southern; D002167:Campylobacter / Q000145:classification* / Q000201:enzymology; D002169:Campylobacter Infections / Q000382:microbiology; D012696:Glycine Hydroxymethyltransferase / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D015345:Oligonucleotide Probes; D012696:Glycine Hydroxymethyltransferase