Determination of amino acids in overlapped capillary electrophoresis peaks by means of partial least-squares regression.
Amino acid derivatives of 1,2-naphthoquinone-4-sulfonate (NQS) can be separated by capillary electrophoresis at 30 kV in a fused-silica capillary by using a 40 mM sodium tetraborate-isopropanol (3:1, v/v) solution as background electrolyte. This procedure was suitable for the most common amino acids. However, the peaks of three amino acids (phenylalanine, isoleucine and tyrosine) were only partially resolved and peaks of histidine and leucine derivatives overlapped completely. Partial least-squares regression (PLS) may overcome the lack of selectivity for these amino acids. Spectroelectropherograms of the corresponding amino acid derivative peaks were monitored with a diode-array spectrophotometer in the range 225 to 540 nm. Both spectra and electropherograms can be used as multivariate data for further analysis. In general, the best predictions were obtained using the time domain.
Latorre
R M
RM
Department of Analytical Chemistry, University of Barcelona, Spain.
Saurina
J
J
Hernández-Cassou
S
S
D000596:Amino Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D016018:Least-Squares Analysis
D000596:Amino Acids