Serum antibody responses of cats to soluble whole cell antigens of feline Porphyromonas gingivalis.
The whole cell soluble antigens of two strains (VPB 3457 and VPB 3492) of feline Porphyromonas gingivalis were analysed by Western blotting using serum taken from 40 domestic cats with various grades of periodontal disease. Five strongly immunogenic protein bands (70, 34, 27, 24 and 19kDa) from VPB 3457 and seven from VPB 3492 (58, 44, 34, 27, 25, 24 and 21kDa) were selected for further study. A significant positive correlation was found between the serum antibody response to the 70, 34, 27, 24 and 19kDa bands of VPB 3457 and the 58, 44, 25, 24 and 21kDa bands of VPB 3492 and the overall periodontal grade. A significant positive correlation was also found between the serum antibody response to the 24kDa band of VPB 3457 and the total colony forming units of P. gingivalis. N-terminal sequencing of the 44kDa band of VPB 3492 showed 75% identity with the translated amino acids from the hag A (haemagglutinin) gene of a human strain of P. gingivalis and N-terminal amino acid sequence of the 27kDa band of VPB 3457 showed 88% identity with the amino acid sequences translated from DNA of purported genes coding for variously named proteinases of human strains of P. gingivalis.
Norris
J M
JM
Department of Veterinary Anatomy and Pathology, University of Sydney, Sydney, Australia.
Love
D N
DN
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D016866:Bacteroidaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D002371:Cat Diseases / Q000276:immunology* / Q000382:microbiology; D002415:Cats; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000276:immunology*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial