title: The quantitative measurement of autoantibodies to thyroglobulin and thyroid peroxidase by automated microparticle based immunoassays in Hashimoto's disease, Graves' disease and a follow-up study on postpartum thyroid disease.
abstract: The aim of this study was to investigate the association between various autoimmune thyroid diseases and the presence of anti-TPO and anti-Tg antibodies using two novel automated microparticle based immunoassays developed for the AxSYM analyzer. Serum samples from 65 individuals with Hashimoto's Disease, 38 with Graves' Disease and 80 UK blood donors were assayed. In addition, samples were taken from 50 women known to be positive for TPO antibodies, for up to 24 weeks following delivery. Precision for both assays ranges from 5.7-9.1% CV, while analytical sensitivity was determined to be 1.0 IU/ml for Anti-Tg and 0.3 IU/ml for Anti-TPO. The Anti-TPO test showed positive results in 86% of Hashimoto's Disease and 87% of Graves' Disease. The figures obtained for Anti-Tg were 58% and 73% respectively. Specificity was 94% with Anti-TPO and 99% with Anti-Tg. The postpartum women were divided into 2 groups, group A remained symptomless while group B developed thyroid dysfunction. Within the 2 groups, medians calculated at each time point were compared between and within groups using the Mann-Whitney Rank Sum Test or the Kruskal-Wallis One Way ANOVA on Ranks. Anti-TPO baseline levels (week 6) were statistically different between both groups (median 36 vs. 167 IU/ml, p = 0.002). In group A, the median values increased from 36 to 87 IU/ml within the observation period, although the difference was not statistically significant. In group B, antibody titres showed a statistically significant increase from 168 IU/ml (week 6) up to 676 IU/ml after 20 weeks (p < 0.001). Anti-Tg baseline levels were not statistically different between the two groups. In group A, the median values did not change significantly over time (range: 47-86 IU/ml) whereas antibody titres in group B showed a statistically significant increase from 79 IU/ml (week 6) to 276 IU/ml after 24 weeks (p = 0.002). Results obtained indicate that these assays provide useful tools for the quantitative determination of autoantibodies in both primary diagnosis as shown with the Hashimoto's disease and Graves' disease samples and patient follow-up as demonstrated with the postpartum samples. The automation and high precision of the assays make them perfectly suited to routine diagnostic use.
authors.affiliation: Axis-Shield Diagnostics Limited, Dundee, Scotland, United Kingdom.
authors.forename: J
authors.initials: J
authors.lastname: Gilmour
authors.forename: Y
authors.initials: Y
authors.lastname: Brownlee
authors.forename: P
authors.initials: P
authors.lastname: Foster
authors.forename: C
authors.initials: C
authors.lastname: Geekie
authors.forename: P
authors.initials: P
authors.lastname: Kelly
authors.forename: S
authors.initials: S
authors.lastname: Robertson
authors.forename: E
authors.initials: E
authors.lastname: Wade
authors.forename: H B
authors.initials: HB
authors.lastname: Braun
authors.forename: U
authors.initials: U
authors.lastname: Staub
authors.forename: G
authors.initials: G
authors.lastname: Michel
authors.forename: J H
authors.initials: JH
authors.lastname: Lazarus
authors.forename: A B
authors.initials: AB
authors.lastname: Parkes
mesh_terms: D001322:Autoanalysis / Q000379:methods; D001323:Autoantibodies / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006111:Graves Disease / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007453:Iodide Peroxidase / Q000276:immunology*; D011644:Puerperal Disorders / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013954:Thyroglobulin / Q000276:immunology*; D013959:Thyroid Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D001323:Autoantibodies; D013954:Thyroglobulin; D007453:Iodide Peroxidase