title: A simplified purification procedure of alpha-lactalbumin from milk using Ca(2+)-dependent adsorption in hydrophobic expanded bed chromatography.
abstract: The technique of expanded bed adsorption is originally designed for a direct recovery of proteins from fermentor feedstocks. In this article we describe the use of expanded bed adsorption for the recovery of alpha-lactalbumins from defatted milk using the hydrophobic Streamline Phenyl gel. alpha-Lactalbumins are Ca(2+)-binding proteins. Upon Ca2+ removal, they undergo a significant conformation change rendering them more hydrophobic. Based on this unique property we develop a protocol for fast and efficient purification of alpha-lactalbumin from milk. The use of this technique results in a reduction of the number of chromatographic purification steps.
authors.affiliation: Interdisciplinary Research Centre, Katholieke Universiteit Leuven, Kortrijk, Belgium. wim.noppe@kulak.ac.be
authors.forename: W
authors.initials: W
authors.lastname: Noppe
authors.forename: P
authors.initials: P
authors.lastname: Haezebrouck
authors.forename: I
authors.initials: I
authors.lastname: Hanssens
authors.forename: M
authors.initials: M
authors.lastname: De Cuyper
mesh_terms: D000327:Adsorption; D000818:Animals; D002118:Calcium / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods*; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D007768:Lactalbumin / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007768:Lactalbumin; D002118:Calcium