title: Modulation of the Ca(2+)-activated Cl(-) channel by 14-3-3epsilon.
abstract: We have previously reported an association of 14-3-3epsilon isoform with calmodulin. Using the voltage-clamp technique, the present study investigated the potential role of 14-3-3 in modulating the Ca(2+)-activated Cl(-) channel (CaCC) endogenously expressed in Xenopus oocytes. Injection of 14-3-3epsilon antisense oligodeoxynucleotides resulted in potentiation of the ionomycin-induced Cl(-) current, while 14-3-3 peptide and calmodulin inhibitor, W13, suppressed the antisense-potentiated current. The data suggest that 14-3-3epsilon plays an inhibitory role in modulating the CaCC by interacting with the calmodulin-dependent pathway. The potential role of 14-3-3epsilon in other tissues and its therapeutic potential for cystic fibrosis are discussed.
authors.affiliation: Epithelial Cell Biology Research Centre, Department of Physiology, Faculty of Medicine, Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
authors.forename: H C
authors.initials: HC
authors.lastname: Chan
authors.forename: W L
authors.initials: WL
authors.lastname: Wu
authors.forename: S C
authors.initials: SC
authors.lastname: So
authors.forename: Y W
authors.initials: YW
authors.lastname: Chung
authors.forename: L L
authors.initials: LL
authors.lastname: Tsang
authors.forename: X F
authors.initials: XF
authors.lastname: Wang
authors.forename: Y C
authors.initials: YC
authors.lastname: Yan
authors.forename: S C
authors.initials: SC
authors.lastname: Luk
authors.forename: S S
authors.initials: SS
authors.lastname: Siu
authors.forename: S K
authors.initials: SK
authors.lastname: Tsui
authors.forename: K P
authors.initials: KP
authors.lastname: Fung
authors.forename: C Y
authors.initials: CY
authors.lastname: Lee
authors.forename: M M
authors.initials: MM
authors.lastname: Waye
mesh_terms: D048948:14-3-3 Proteins; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D065101:Chloride Channel Agonists; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase; D014981:Xenopus
chemical_list: D048948:14-3-3 Proteins; D065101:Chloride Channel Agonists; D018118:Chloride Channels; D016376:Oligonucleotides, Antisense; D011506:Proteins; D015759:Ionomycin; D014446:Tyrosine 3-Monooxygenase; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D002118:Calcium