title: Close association of the N terminus of Kv1.3 with the pore region.
abstract: The Shaker superfamily encodes voltage-gated potassium (Kv) channels. The N termini of Shaker proteins are located intracellularly and contain several domains shown to regulate important aspects of channel function, such as speed of inactivation, channel assembly (T1 domain), and steady state protein level (T0 domain, amino acids 3-39 in rabbit). Mutations and/or deletion of certain amino acids in the T0 domain lead to a 13-fold amplification of Kv current as compared with wild type channels, primarily by increasing the absolute number of channel proteins present in the membrane (Segal, A. S., Yao, X., and Desir, G. V. (1999) Biochem. Biophys. Res. Commun. 254, 54-64). Although T0 mutants have kinetic properties virtually indistinguishable from wild type, they were noted to have a slightly larger single channel conductance, suggesting that the T0 domain might also interact with the pore region. In the present study we show that although T0 does not affect pore selectivity, it does modulate the binding affinity of the pore blocker, charybdotoxin. These results suggest that the N terminus of Kv1.3 is closely associated with the pore region.
authors.affiliation: Department of Physiology, Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: X
authors.initials: X
authors.lastname: Yao
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Tian
authors.forename: H
authors.initials: H
authors.lastname: Rafi
authors.forename: A S
authors.initials: AS
authors.lastname: Segal
authors.forename: G V
authors.initials: GV
authors.lastname: Desir
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018999:Charybdotoxin / Q000494:pharmacology; D051664:Kv1.3 Potassium Channel; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D015221:Potassium Channels / Q000737:chemistry*; D024642:Potassium Channels, Voltage-Gated; D011817:Rabbits; D014982:Xenopus laevis
chemical_list: D051664:Kv1.3 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D018999:Charybdotoxin