Determination of a new asiatic acid derivative, AS 2-006A in rat plasma and urine, and human plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic method was developed for the determination of a new wound healing agent, AS 2-006A (ethoxymethyl 2-oxo-3, 23-O-isopropylideneasiatate), in rat plasma and urine, and human plasma. The sample preparation was simple: 2 volumes of acetonitrile were added to the biological samples to deproteinize it. A 50-microl aliquot of the supernatant was injected onto the reversed-phase column. The mobile phase employed was acetonitrile : H2O (9:1, v/v) and run at a flow rate of 1.1 ml/min. The column effluent was monitored by a UV detector set at 205 nm. The retention time for AS 2-006A was approximately 29.5 min. The detection limits for AS 2-006A in rat and human plasma were both 1 microg/ml, and in rat urine was 2 microg/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 10.8%) for rat plasma and urine, and human plasma. No interferences from endogenous substances were found.
Kim
G B
GB
College of Pharmacy, Seoul National University, South Korea.
Kim
Y G
YG
Jew
S S
SS
Lee
M G
MG
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D053978:Pentacyclic Triterpenes; D051381:Rats; D017207:Rats, Sprague-Dawley; D014315:Triterpenes / Q000032:analysis* / Q000097:blood / Q000737:chemistry* / Q000493:pharmacokinetics / Q000652:urine
C406665:AS 2-006A; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C017032:asiatic acid