[Neuronal expression of foreign genes with recombinant rabies virus variants].
Rabies virus variants obtained by recombinant DNA techniques enabled us to use the high neurotropism of rabies virus to express foreign genes (e.g: Chloramphenicol Acetyl Transferase gene) in neuronal cell cultures as well as in rodent brain. The foreign gene was inserted in the viral pseudogene region; this insertion did not affect the neurotropism of rabies virus, as shown by infection of neuronal cell cultures without any major cytopathic effects for several days. Stereotaxic inoculation of these rabies virus variants into rat striatum indicated that insertion of the foreign gene did not alter the viral axonal transport and the subsequent widespread brain infection. These data allow to consider rabies virus as a vector for the selective expression of foreign genes in neurons.
Etessami
R
R
Unité de la Rage, Institut Pasteur, Paris, France.
Conzelmann
K K
KK
Marion
R
R
Tsiang
H
H
Ceccaldi
P E
PE
D000818:Animals; D001921:Brain / Q000276:immunology / Q000473:pathology / Q000821:virology*; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics / Q000276:immunology; D014644:Genetic Variation; D005822:Genetic Vectors / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D009474:Neurons / Q000276:immunology* / Q000821:virology; D011544:Pseudogenes / Q000235:genetics; D011819:Rabies Vaccines / Q000235:genetics / Q000276:immunology; D011820:Rabies virus / Q000235:genetics* / Q000276:immunology; D051381:Rats; D014158:Transcription, Genetic / Q000235:genetics; D018523:Tropism / Q000235:genetics; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology
D004251:DNA Transposable Elements; D011819:Rabies Vaccines; D019444:Vaccines, DNA; D015500:Chloramphenicol O-Acetyltransferase