The structure and expression of the murine wildtype p53-induced phosphatase 1 (Wip1) gene.
The human wildtype p53-induced phosphatase 1 (Wip1; GenBank symbol Ppm1d) gene encodes a type 2C protein phosphatase (PP2C) that is induced by ionizing radiation in a p53-dependent manner. We have cloned and sequenced the mouse Wip1 gene and its encoded mRNA. The mouse Wip1 gene is composed of six exons and spans over 36 kb of DNA. The mouse cDNA sequence predicts a 598-amino-acid protein with a molecular mass of roughly 66 kDa. Comparison of human and mouse Wip1 sequences revealed 83% overall identity at the amino acid level. The 5'-flanking region of exon 1 had promoter elements characteristic of a housekeeping gene. The Wip1 coding sequences share conserved functional regions with other PP2Cs from a diverse array of species. Expression of Wip1 mRNA was detected ubiquitously in adult and embryonic tissues, though expression in the testis was much higher than in other tissues. Wip1 has been mapped near the p53 gene on mouse chromosome 11.
Choi
J
J
Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 77030, USA.
Appella
E
E
Donehower
L A
LA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D005333:Fetus / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009928:Organ Specificity; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D016159:Tumor Suppressor Protein p53; C000607458:PPM1D protein, human; C087358:PTC1 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; C000607468:Ppm1d protein, mouse; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C