title: The effects of RAD52 epistasis group genes on various types of spontaneous mitotic recombination in Saccharomyces cerevisiae.
abstract: The role of RAD52 epistasis group genes on spontaneous mitotic recombination was examined using three different types of spontaneous mitotic recombination in Saccharomyces cerevisiae. The spontaneous recombination between homologous sequences in a plasmid and a chromosome was essentially unaffected by null mutations in any of the RAD52 epistasis group genes. Recombination between genes in separate autonomously replicating plasmids was reduced 833-fold in a rad52 null mutant, but only 2- to at most 20-fold in rad50, 51, 54, 55, 57 null mutants. Recombination between tandemly repeated heteroalleles in an autonomously replicating plasmid was reduced almost 100-fold in a rad52 null mutant, but is either unaffected or slightly increased in rad50, 51, 54, 55, 57 null mutants. The finding that RAD50, 51, 54, 55, 57 are dispensable or marginally involved in these spontaneous recombinations suggests further that spontaneous mitotic recombination in S. cerevisiae might be processed by other than RAD52 epistasis group.
authors.affiliation: Department of Pathology, College of Medicine, Catholic University of Korea, Seoch-gu Banpo-dong 505, Seoul, 137-701, Korea. jiyou@cmc.cuk.ac.kr
authors.forename: J C
authors.initials: JC
authors.lastname: You
mesh_terms: D000483:Alleles; D015825:Chromosomes, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004843:Epistasis, Genetic; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008938:Mitosis / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D051721:Rad52 DNA Repair and Recombination Protein; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins