Human immunodeficiency virus type 1 p24 concentration measured by boosted ELISA of heat-denatured plasma correlates with decline in CD4 cells, progression to AIDS, and survival: comparison with viral RNA measurement.
Human immunodeficiency virus type 1 (HIV-1) RNA and p24 antigen concentrations were determined in plasma samples from 169 chronically infected patients (median CD4 cell count, 140 cells/microL; range, 0-1500 cells/microL). p24 quantification involved heat-mediated immune complex dissociation and tyramide signal amplification-boosted ELISA, which has a diagnostic sensitivity similar to that of RNA quantification by a commercial polymerase chain reaction kit. In Cox's proportional hazard models adjusted for CD4 cell count, both RNA (P<.005) and p24 (P=.043) levels were significant predictors of progression to AIDS. Measurement of p24 was superior to measurement of RNA in the model for survival (P=.032 vs. P=.19). p24 level was a significant predictor of CD4 cell decline in models adjusted for CD4 cell counts and was superior or equivalent to RNA level, depending on the group analyzed. Stratification by CD4 cell counts at baseline showed that the superiority of p24 measurement was more pronounced at lower levels of CD4 cells (<200/microL). p24 level may be of interest as a simple and inexpensive predictive marker of disease progression.
Ledergerber
B
B
Division of Infectious Diseases, Department of Medicine, Zurich University Hospital, Zurich, Switzerland.
Flepp
M
M
Böni
J
J
Tomasik
Z
Z
Cone
R W
RW
Lüthy
R
R
Schüpbach
J
J
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D015415:Biomarkers; D018791:CD4 Lymphocyte Count; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016655:HIV Core Protein p24 / Q000097:blood*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D008297:Male; D011489:Protein Denaturation; D012367:RNA, Viral / Q000032:analysis; D012307:Risk Factors; D015996:Survival Rate; D019562:Viral Load
D015415:Biomarkers; D016655:HIV Core Protein p24; D012367:RNA, Viral