title: Electrotransformation of Clostridium acetobutylicum ATCC 824 using high-voltage radio frequency modulated square pulses.
abstract: Molecular biological improvement of industrial solventogenic clostridia could be enhanced by a higher efficiency of electrotransformation. In this research, we used a new approach to determine the frequency spontaneously generated by Clostridium acetobutylicum ATCC 824 cells during the application of a square high-voltage pulse. Once the frequency of 100 kHz was determined we transformed clostridial cells with pSOS84 plasmid DNA using radio-frequency modulated high-voltage square pulses (electric field strength 12 kVcm-1; pulse duration 22.5 ms; frequency of pulse modulation 100 kHz) to reach an efficiency exceeding 106 transformants microg-1 of plasmid DNA. We propose a possible role for cellular membrane structures in affecting the transformation yield.
authors.affiliation: Department of Biochemistry and Cell Biology, and Department of Ecology and Evolutionary Biology, Rice University, Houston, TX 77251-1892, USA.
authors.forename: M
authors.initials: M
authors.lastname: Tyurin
authors.forename: R
authors.initials: R
authors.lastname: Padda
authors.forename: K X
authors.initials: KX
authors.lastname: Huang
authors.forename: S
authors.initials: S
authors.lastname: Wardwell
authors.forename: D
authors.initials: D
authors.lastname: Caprette
authors.forename: G N
authors.initials: GN
authors.lastname: Bennett
mesh_terms: D003013:Clostridium / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D003470:Culture Media; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D018274:Electroporation / Q000379:methods*; D008854:Microscopy, Electron; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D003470:Culture Media