Biotransformation of the Trichoderma metabolite 6-n-pentyl-2H-pyran-2-one by cell suspension cultures of Pinus radiata.
Cell suspension cultures of Pinus radiata metabolize the antifungal Trichoderma secondary metabolite 6-n-pentyl-2H-pyran-2-one (6PAP) (1) via hydroxylation of the pentyl side chain. Examination of the culture medium following dosing studies with 1 revealed that 79-85% of this bioactive compound had been metabolised after 144 h. At that time, 34-40% of the metabolized dose was recovered as a series of monohydroxylated isomers of 1, the principal metabolite being 5-(2-pyron-6-yl)pentan-5-ol (7).
Cooney
J M
JM
The Horticultural and Food Research Institute Ltd., Ruakura Research Centre, Hamilton, New Zealand. jcooney@hort.cri.nz
Hotter
G S
GS
Lauren
D R
DR
D000935:Antifungal Agents / Q000378:metabolism*; D001711:Biotransformation; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D011753:Pyrones / Q000378:metabolism*; D014197:Trees / Q000378:metabolism*; D014242:Trichoderma / Q000378:metabolism*
C107766:6-n-pentyl-2H-pyran-2-one; D000935:Antifungal Agents; D011753:Pyrones