Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons.
The results reported herein address the question of synaptogenesis between adrenal chromaffin cells and striatal neurons. The release of dopamine from chromaffin cells in the presence of striatal neurons was also examined. Co-culture of newborn rat chromaffin cells and striatal neurons at 1:1 ratio was made. Cultures were examined morphologically using immunocytochemistry and ultrastructural techniques (transmission electron microscopy), while quantitation of dopamine in the culture media by HPLC-ECD was also determined. Neurite outgrowth from chromaffin cells was enhanced in the presence of striatal neurons and numerous synaptic-like contacts between these two cell types were observed. Higher concentration of dopamine was also present in the co-culture medium as compared with those containing only chromaffin cells. The development of synapses between these two types of cells may give support to the functionality of transplants in human cases of Parkinson disease (PD).
Zhang
L
L
Depto. Fisiología, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico City.
Castell
A
A
Avila
E
E
Drucker-Colín
R
R
Escobar
A
A
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques; D003342:Corpus Striatum / Q000166:cytology; D017077:Culture Media, Conditioned / Q000737:chemistry; D004298:Dopamine / Q000032:analysis / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009474:Neurons / Q000166:cytology*; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure
D017077:Culture Media, Conditioned; D016650:Fluorescein-5-isothiocyanate; D004298:Dopamine; D009638:Norepinephrine