Determination of alpha- and beta-trenbolone in bovine muscle and liver by liquid chromatography with fluorescence detection.
A sensitive and rapid high-performance liquid chromatographic screening method is described for the determination of anabolic steroid trenbolone in bovine muscle and liver. Trenbolone was analyzed as alpha- and beta-trenbolone. Samples were extracted with ethyl acetate and cleaned up on a silica solid-phase extraction (SPE) cartridge. Liver samples were cleaned up on a multifunctional SPE cartridge before using a silica SPE cartridge. Analysis of alpha- and beta-trenbolone was performed by reversed-phase high-performance liquid chromatography (HPLC) with a fluorescence detector. The detection limits for this method were estimated to be 0.2 and 1.0 ng/g in bovine muscle and liver, respectively. The mean recoveries spiked in muscle at 2 ng/g and in liver at 10 ng/g were over 80%.
Yoshioka
N
N
Hyogo Prefectural Institute of Public Health, Kobe, Japan. yoshioka@iph.pref.hyogo.jp
Akiyama
Y
Y
Takeda
N
N
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014204:Trenbolone Acetate / Q000031:analogs & derivatives* / Q000032:analysis
D014204:Trenbolone Acetate