title: Octylcyanoacrylate for the treatment of contaminated partial-thickness burns in swine: a randomized controlled experiment.
abstract: OBJECTIVE
  To compare infection and reepithelialization rates of contaminated second-degree burns treated with octylcyanoacrylate (OCA), silver sulfadiazine (SSD), polyurethane (PU) film, and dry gauze (control; C) in swine.
  
  
  METHODS
  Eighty standardized burns were created by applying an aluminum bar preheated to 80 degrees C to the backs and flanks of young pigs for 20 seconds. All burns were immediately contaminated with 0.1 mL of Staphylococcus aureus 10(5)/mL and randomly treated with OCA spray, SSD, PU, or dry gauze (C). Full-thickness biopsies were taken at 3, 7, and 14 days for blinded histopathologic evaluation using hematoxylin and eosin (H&E)-stained slides. Burns were considered infected in the presence of interstitial reticular dermal neutrophils containing bacteria (intraobserver agreement, kappa = 1.00). Quantitative wound cultures were performed on a second day-3 specimen.
  
  
  RESULTS
  At day 3, wound infection rates were 30% (OCA), 50% (SSD), 55% (PU), and 50% (C); p = 0.40 (Kruskal-Wallis test). At day 7, infection rates were 35% (OCA), 85% (SSD), 70% (PU), and 65% (C); p = 0.01. Median bacterial counts at day 3 were 6,500 (OCA), 20,000 (SSD), 1,000,000 (PU), and 650,000 (C); p = 0.29. The proportion of completely reepithelialized wounds at day 14 were 75% (OCA), 90% (SSD), 85% (PU), and 90% (C); p = 0.50.
  
  
  CONCLUSIONS
  Treatment of contaminated partial-thickness burns with OCA spray resulted in fewer infections at one week than with the other three treatments.
authors.affiliation: Department of Emergency Medicine, State University of New York, Stony Brook 11794-7400, USA. asinger@epo.som.sunysb.edu
authors.forename: A J
authors.initials: AJ
authors.lastname: Singer
authors.forename: M
authors.initials: M
authors.lastname: Mohammad
authors.forename: G
authors.initials: G
authors.lastname: Tortora
authors.forename: H C
authors.initials: HC
authors.lastname: Thode
authors.forename: S A
authors.initials: SA
authors.lastname: McClain
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001458:Bandages; D002056:Burns / Q000188:drug therapy* / Q000382:microbiology; D003487:Cyanoacrylates / Q000627:therapeutic use*; D003646:Debridement; D005260:Female; D013411:Sulfadiazine / Q000627:therapeutic use; D013552:Swine; D016896:Treatment Outcome; D014946:Wound Infection / Q000188:drug therapy*
chemical_list: D000900:Anti-Bacterial Agents; D003487:Cyanoacrylates; C119247:octylcyanoacrylate; D013411:Sulfadiazine