Plasma progesterone profiles and fertility status of anestrus Zebu cattle treated with norgestomet-estradiol-eCG regimen.
Zebu cattle are notorious for poor fertility characterized by late maturity and long intercalving intervals attributed to a variety of factors, including genetic, nutritional and climatic. The aim of the present investigation, therefore, was to induce fertile estrus in acyclic pubertal heifers and postpartum anestrous Zebu cows by hormonal intervention. Pubertal Hariana and Sahiwal anestrous heifers (n = 51) and postpartum cows (n = 55) were either assigned a placebo (controls, n = 6 for each breed and parity) or treated with 10-d norgestomet (3 mg) subcutaneous ear implants, with an initial injection of 3 mg, i.m. norgestomet + 5 mg estradiol valerate, followed by 500 IU eCG at implant withdrawal (NOR-treated groups). Jugular venous plasma samples were obtained from a total of 28 animals (controls: 4 heifers and 4 cows; NOR-treated: 12 heifers and 8 cows) on Days 0 (implant insertion), 3, 7, 9 and Day 10 (implant withdrawal), every 12 h on Days 11 and 12, and then once daily on Days 17, 24 and 31. All the samples were assayed for progesterone. Almost all (97%) heifers and 81% cows were induced to estrus, the majority (92% heifers and 79% cows) within 120 h of implant removal. Synchrony of the induced estrus was better in cows, but interval to estrus and estrus duration were significantly longer in heifers (P < 0.05). Post-treatment fertility, based on Day 28 nonreturn rate, first service, and overall conception rates, was better in heifers (78.9, 60.5 and 73.7%, respectively) than cows (77.1, 48.6 and 62.9%, respectively), but the differences were significant only for the overall pregnancy rate (71.8% for heifers and 51.2% for cows; P < 0.05). Low pre-treatment plasma progesterone values (< 0.5 ng/mL) were consistent with ovarian inactivity, confirming the true anestrus status of experimental animals. Controls failed to exhibit estrus and maintained low progesterone concentrations throughout the study. In treated animals, high progesterone values from Day 17 onwards suggested ovulatory estrus. These early luteal phase progesterone concentrations in nonpregnant (P = 0.06) and nonpregnant, nonreturn (P < 0.05) animals were low in comparison with those of pregnant animals. Good fertility resulting from breeding according to estrus, inspite of variable intervals to estrus and estrus duration, advocates its advantage over fixed-time insemination in norgestomet-treated anestrous Zebu cattle.
Singh
U
U
Department of Gynaecology & Obstetrics, College of Veterinary Sciences, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India.
Khurana
N K
NK
Inderjeet
D000780:Anestrus; D000818:Animals; D002417:Cattle / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage*; D004343:Drug Implants; D004958:Estradiol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D004971:Estrus; D005260:Female; D005298:Fertility; D007700:Kinetics; D011247:Pregnancy; D011282:Pregnenediones / Q000008:administration & dosage*; D011374:Progesterone / Q000097:blood*; D045167:Progesterone Congeners / Q000008:administration & dosage
D006063:Chorionic Gonadotropin; D004343:Drug Implants; D011282:Pregnenediones; D045167:Progesterone Congeners; C100284:norgestomet; D011374:Progesterone; D004958:Estradiol