Isolation and characterization of a Pseudomonas sp. strain PH1 utilizing meta-aminophenol.
Pseudomonas sp. strain PH1 was isolated from soil contaminated with pharmaceutical and dye industry waste. The isolate PH1 could use m-aminophenol as a sole source of carbon, nitrogen, and energy to support the growth. PH1 could degrade up to 0.32 mM m-aminophenol in 120 h, when provided as nitrogen source at 0.4 mM concentration with citrate (0.5 mM) as a carbon source in the growth medium. The presence of ammonium chloride as an additional nitrogen source repressed the degradation of m-aminophenol by PH1. To identify strain PH1, the 16S rDNA sequence was amplified by PCR using conserved eubacterial primers. The FASTA program was used to analyze the 16S rDNA sequence and the resulting homology patterns suggested that PH1 is a Pseudomonas.
Kutty
R
R
National Environmental Engineering Research Institute, Nagpur, India.
Purohit
H J
HJ
Khanna
P
P
D000627:Aminophenols / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D007220:Industrial Waste; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
D000627:Aminophenols; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C055528:3-aminophenol; D010100:Oxygen