title: Changing subcellular distribution and activity-dependent utilization of a dendritically localized mRNA in developing Purkinje cells.
abstract: In cerebellar Purkinje neurons, the degree of dendritic segregation of the Purkinje cell-specific mRNA L7/pcp-2 is correlated with their development and synaptic investment. This developmental pattern is also observed in Purkinje cells in primary dissociated culture. Short-term (12-48 h) stimulation of cultured Purkinje cells by potassium-induced depolarization or blockade of their inhibitory GABAergic input results in an increased incidence of Purkinje cells with L7/pcp-2 mRNA-positive dendrites and increased levels of L7 protein expression, the latter by a posttranscriptional mechanism. None of these treatments affected the localization of the mRNA encoding calbindin D28k nor the level of this protein in Purkinje neurons. Protracted exposure to depolarizing levels of potassium or elimination of GABAergic transmission resulted in conspicuous changes of Purkinje cell dendritic morphology. These data suggest a scenario in which activity-driven translation of subcellularly segregated mRNAs may contribute to the developmental and functional plasticity of nerve cells.
authors.affiliation: Abteilung Anatomie und Zellbiologie, Universität Ulm, Ulm, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Wanner
authors.forename: S L
authors.initials: SL
authors.lastname: Baader
authors.forename: J
authors.initials: J
authors.lastname: Oberdick
authors.forename: K
authors.initials: K
authors.lastname: Schilling
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development*; D003609:Dactinomycin / Q000494:pharmacology; D003712:Dendrites / Q000378:metabolism* / Q000648:ultrastructure; D018756:GABA Antagonists / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D020662:Guanine Nucleotide Exchange Factors; D051379:Mice; D009473:Neuronal Plasticity; D009479:Neuropeptides / Q000235:genetics*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D010852:Picrotoxin / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011689:Purkinje Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis*; D013347:Subcellular Fractions / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D003609:Dactinomycin; D018756:GABA Antagonists; D020662:Guanine Nucleotide Exchange Factors; D009479:Neuropeptides; D019384:Nucleic Acid Synthesis Inhibitors; D010852:Picrotoxin; D011188:Potassium; D012333:RNA, Messenger; D005680:gamma-Aminobutyric Acid; C104971:Pcp2 protein, mouse