title: O-(N-succinimidyl)-1,1,3,3-tetramethyluronium tetrafluoroborate-N-hydroxysuccinimide-CuCl2: a facile and reliable system for racemization-free coupling of peptides having a carboxy-terminal N-methylamino acid.
abstract: Simultaneous use of N-hydroxysuccinimide (HOSu) and CuCl2 with a HOSu-based uronium coupling reagent, O-(N-succinimidyl)-1,1,3,3-tetramethyluronium tetrafluoroborate, has been found to eliminate the racemization of the carboxy-terminal N-methylamino acid residue during the segment condensation.
authors.affiliation: Department of Industrial Chemistry, Faculty of Engineering, Seikei University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: S
authors.initials: S
authors.lastname: Ishizuka
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Kurita
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D007202:Indicators and Reagents; D008782:Methylurea Compounds / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013388:Succinimides / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D007202:Indicators and Reagents; D008782:Methylurea Compounds; C087069:N,N,N',N'-tetramethyl(succinimido)uronium tetrafluoroborate; D010455:Peptides; D013388:Succinimides; D003300:Copper; C001426:N-hydroxysuccinimide; C029892:cupric chloride