Association of EBV strains, defined by multiple loci analyses, in non-Hodgkin lymphomas and reactive tissues from HIV positive and HIV negative patients.
Epstein-Barr virus (EBV) associated with lymphoid neoplasms demonstrates preferential association with certain viral strains. Previous subtyping studies have however been confined to analysis of sequence variability within a single locus in EBV. Variations have now been reported for several latently expressed EBV genes, including, EBNAs-1, 2 and LMP-1. Variant EBNA-1 strains have been identified in Burkitt's lymphomas and clustering of subtypes for LMP and EBNA-2 have been associated with either malignancy and/or clinical disease. To investigate the linkage between the variability in these three loci in EBV associated with lymphoid malignancies, we subclassified EBV-associated lymphoproliferations (9 reactive and 24 malignant) from HIV-negative and HIV-positive patients by analysis of the EBNA-1, LMP1, and EBNA-2 genes. Our results demonstrate that (1) EBV identical to the prototype B95.8 strain (Type 1 EBNA-2, wild type EBNA-1 and LMP-1) is very rarely associated with tumors. (2) The EBNA-1 variant V-leucine, restricted to malignant lymphomas in immunocompetent patients, was readily identified in non-malignant lesions in HIV infected patients. (3) Variations of EBNA-1 occur independent of variations at other loci.
Gutiérrez
M I
MI
Lymphoma Biology Section, Pediatric Branch, Division of Clinical Sciences, National Cancer Institute, NIH, Bethesda, MD, USA.
Kingma
D W
DW
Sorbara
L
L
Tran
M
M
Raffeld
M
M
Jaffe
E S
ES
Magrath
I
I
Bhatia
K
K
D000956:Antigens, Viral / Q000235:genetics; D002051:Burkitt Lymphoma / Q000235:genetics / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000821:virology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D018023:HIV Seronegativity / Q000235:genetics; D006679:HIV Seropositivity / Q000235:genetics / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008223:Lymphoma / Q000821:virology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000821:virology
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D005819:Genetic Markers