A tetrodotoxin-producing Vibrio strain, LM-1, from the puffer fish Fugu vermicularis radiatus.
Identification of tetrodotoxin (TTX) and its derivatives produced from a Vibrio strain in the intestine of the puffer fish Fugu vermicularis radiatus was performed by thin-layer chromatography, electrophoresis, high-performance liquid chromatography, and gas chromatography-mass spectrometry, together with a mouse bioassay for toxicity. It was demonstrated that the isolated bacterium produced TTX, 4-epi-TTX, and anhTTX during cultivation, suggesting that Vibrio strains are responsible for the toxification of the puffer fish.
Lee
M J
MJ
Department of Diagnosis, Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, Kyung-Ju 780-714, Korea.
Jeong
D Y
DY
Kim
W S
WS
Kim
H D
HD
Kim
C H
CH
Park
W W
WW
Park
Y H
YH
Kim
K S
KS
Kim
H M
HM
Kim
D S
DS
D000818:Animals; D001681:Biological Assay; D003470:Culture Media; D005400:Fishes, Poisonous / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology; D051379:Mice; D013779:Tetrodotoxin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D014733:Vibrio / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
D003470:Culture Media; D013779:Tetrodotoxin