Effect of ionizing radiation on the differentiation of ROS 17/2.8 osteoblasts through free radicals.
Although the acceleration of bone regeneration by radiation has been reported, the mechanisms of action of radiation on bone are unclear. The present results indicate that ionizing radiation-stimulated differentiation could result from the generation of reactive oxygen species during radiation exposure. The free radical release is considered as the most important mechanism of bone effect by radiation treatment. In addition, we report that radiation induced transient activation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) activation and the transcription factor, AP-1. The JNK and AP-1 activation is mediated with radiation-released free radicals in ROS 17/2.8 osteoblasts. These results indicate that ionizing radiation at a single dose of up to 5 Gray stimulates differentiation of ROS 17/2.8 osteoblasts via free radial release which may affect JNK/SAPK and AP-1 activities.
Chae
H J
HJ
Department of Dental Pharmacology, School of Dentistry, Wonkwang University, Iksan, Chonbuk, Korea.
Chae
S W
SW
Kang
J S
JS
Bang
B G
BG
Han
J I
JI
Moon
S R
SR
Park
R K
RK
So
H S
HS
Jee
K S
KS
Kim
H M
HM
Kim
H R
HR
D000818:Animals; D002454:Cell Differentiation / Q000528:radiation effects*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000528:radiation effects; D005609:Free Radicals; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases / Q000528:radiation effects; D010006:Osteoblasts / Q000528:radiation effects*; D011839:Radiation, Ionizing; D051381:Rats; D018808:Transcription Factor AP-1 / Q000528:radiation effects
D005609:Free Radicals; D018808:Transcription Factor AP-1; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases