[Kinetics of the reaction of nitroblue tetrazolium reduction by human blood neutrophils].
Kinetics of Nitro blue tetrazolium (NBT) reduction to diformasan by neutrophils was investigated using 27 samples of human blood. Analysis of alteration in the share of activated neutrophils (ANP) and activated neutrophil index (ANI) was done in relation to the reaction time. The former reaction is an irreversible reaction of zero (pseudozero) order, while the latter is an irreversible reaction of the first (pseudofirst) order. It has been found out that an induced NBT reduction occurs in parallel with a spontaneous reaction, and that neutrophils have essentially different oxidizing power. The kinetic approach enabled us to discover some indices (NBT quantity involved in the reaction, and reaction speed constant of the first order) which in different samples varied within broader limits than ANP or ANI (within the limits of an order), i.e. provided a possibility to make a more delicate analysis of processes in neutrophils.
Gerasimov
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IG
Research Institute of Family Problems, Donetsk Medical University, Donetsk.
Kalutskaia
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OA
D000328:Adult; D005260:Female; D006801:Humans; D007700:Kinetics; D018375:Neutrophil Activation; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000737:chemistry* / Q000378:metabolism
D009580:Nitroblue Tetrazolium