Cloning, sequence analysis and expression of the cDNAs encoding the canine and equine homologues of the mouse double minute 2 (mdm2) proto-oncogene.
The mdm2 oncogene is amplified and overexpressed in a variety of human tumours and the oncogenic potential of MDM2 is partly due to its ability to inactivate tumour suppressor p53 function. In the present communication we describe the cloning, sequence analysis and expression of the complete wildtype canine and equine mdm2 cDNAs. The encoded full-length canine and equine cDNAs show strong sequence homology with MDM2 proteins from other species and both cDNAs generate recombinant proteins of approximately 90 kDa. These data will allow for the role of this oncogene to be established in companion animal oncology.
Nasir
L
L
Molecular Therapeutics Research Group, Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden Road, Glasgow, UK. lnasir@udcf.gla.ac.uk
Burr
P D
PD
McFarlane
S T
ST
Gault
E
E
Thompson
H
H
Argyle
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004285:Dogs; D006736:Horses; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D051736:Proto-Oncogene Proteins c-mdm2; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; C497207:Mdm2 protein, mouse; D051736:Proto-Oncogene Proteins c-mdm2