Retroviral vector targeting to human immunodeficiency virus type 1-infected cells by receptor pseudotyping.
We report the generation of retroviral vectors based on Moloney murine leukemia virus that specifically transduce cells infected with T-cell-tropic human immunodeficiency virus type 1 (HIV-1). This vector was pseudotyped with T-cell-tropic HIV-1 receptors CD4 and CXCR4. We demonstrate that transduction is contingent upon HIV-1 gp120 and gp41 expression.
Somia
N V
NV
Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Miyoshi
H
H
Schmitt
M J
MJ
Verma
I M
IM
D000818:Animals; D015704:CD4 Antigens / Q000235:genetics / Q000378:metabolism*; D018390:Gene Targeting; D005822:Genetic Vectors / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000378:metabolism*; D015700:HIV Envelope Protein gp41 / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D019718:Receptors, CXCR4