Association of bovine embryos produced by in vitro fertilization with a noncytopathic strain of bovine viral diarrhea virus type II.
In the first experiment, heifers were infected experimentally with bovine viral diarrhea virus type II (BVDV-type II, strain CD87; characterized by high morbidity and mortality). Subsequently, in vitro fertilized embryos were produced from oocytes collected on Day 4, 8, and 16 post infection. In a total of 29 heifers, the infectious virus was detected in 55% of the samples of the follicular fluid, in 10% of the oviductal cells, in 10% of the uterine flushes and in 41% of the in vitro fertilized embryos. The highest number of embryos associated with the virus was detected in the group of animals slaughtered on Day 8 post infection (58%). The amount of the virus (10(1.5-2.0) TCID50/mL) associated with the washed single embryos generated from oocytes of heifers 8 and 16 d post infection was sufficient for disease transmission by intravenous inoculation to the seronegative recipients (6/15). In the second experiment, uninfected oocytes were exposed in vitro to BVDV (10(5) TCID50/mL) in the maturation medium and then fertilized and cultured prior to viral assay. Virus was detected in 4 of 7 samples containing embryos but not in samples of embryos produced from the control group of uninfected oocytes. The presence of BVDV in the IVF system did not affect embryonic development in vitro. In conclusion, it appears that BVDV-type II has the ability to be transferred with oocytes through the IVF system, resulting in infectious embryos with normal morphological appearance which may have a potential for disease transmission.
Bielanski
A
A
Canadian Food Inspection Agency, Animal Diseases Research Institute, Ontario, Canada.
Sapp
T
T
Lutze-Wallace
C
C
D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000196:embryology* / Q000473:pathology / Q000635:transmission; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D018920:Coculture Techniques; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification / Q000472:pathogenicity*; D004622:Embryo, Mammalian / Q000821:virology; D005187:Fallopian Tubes / Q000821:virology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D015571:Follicular Fluid / Q000821:virology; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D009865:Oocytes / Q000821:virology; D011247:Pregnancy; D014599:Uterus / Q000821:virology