title: A rationalization of the acidic pH dependence for stromelysin-1 (Matrix metalloproteinase-3) catalysis and inhibition.
abstract: The pH dependence of matrix metalloproteinase (MMP) catalysis is described by a broad bell-shaped curve, indicating the involvement of two unspecified ionizable groups in proteolysis. Stromelysin-1 has a third pK(a) near 6, resulting in a uniquely sharp acidic catalytic optimum, which has recently been attributed to His(224). This suggests the presence of a critical, but unidentified, S1' substructure. Integrating biochemical characterizations of inhibitor-enzyme interactions with active site topography from corresponding crystal structures, we isolated contributions to the pH dependence of catalysis and inhibition of active site residues Glu(202) and His(224). The acidic pK(a) 5.6 is attributed to the Glu(202).zinc.H(2)O complex, consistent with a role for the invariant active site Glu as a general base in MMP catalysis. The His(224)-dependent substructure is identified as a tripeptide (Pro(221)-Leu(222)-Tyr(223)) that forms the substrate cleft lower wall. Substrate binding induces a beta-conformation in this sequence, which extends and anchors the larger beta-sheet of the enzyme. substrate complex and appears to be essential for productive substrate binding. Because the PXY tripeptide is strictly conserved among MMPs, this "beta-anchor" may represent a common motif required for macromolecular substrate hydrolysis. The striking acidic profile of stromelysin-1 defined by the combined ionization of Glu(202) and His(224) allows the design of highly selective inhibitors.
authors.affiliation: Department of Cancer Research, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Co., Ann Arbor, Michigan 48105, USA. Linda.Johnson@wl.com
authors.forename: L L
authors.initials: LL
authors.lastname: Johnson
authors.forename: A G
authors.initials: AG
authors.lastname: Pavlovsky
authors.forename: A R
authors.initials: AR
authors.lastname: Johnson
authors.forename: J A
authors.initials: JA
authors.lastname: Janowicz
authors.forename: C F
authors.initials: CF
authors.lastname: Man
authors.forename: D F
authors.initials: DF
authors.lastname: Ortwine
authors.forename: C F
authors.initials: CF
authors.lastname: Purchase
authors.forename: A D
authors.initials: AD
authors.lastname: White
authors.forename: D J
authors.initials: DJ
authors.lastname: Hupe
mesh_terms: D001665:Binding Sites; D020134:Catalytic Domain; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019278:Matrix Metalloproteinase 3 / Q000737:chemistry / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors; D017433:Protein Structure, Secondary
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D019278:Matrix Metalloproteinase 3