[Adaptation of acrylamide producer Rhodococcus rhodochrous M8 to change in ammonium concentration in medium].
The mechanism of adaptation of the acrylamide producing strain Rhodococcus rhodochrous M8 to changes in ammonium concentrations in the medium was studied. An increase in the content of ammonium in the medium changed the activity of glutamine synthetase (GS) (EC 6.3.1.2) and glutamine dehydrogenase (GD) (EC 1.4.1.4), the enzymes of ammonium assimilation, as well as the activities of enzymes responsible for nitrile utilization: nitrile hydratase (EC 4.2.1.84) and amidase (EC 3.5.1.4). This also caused inhibition of activation of GS induced by phosphodiesterase (EC 3.1.4.1). Increases in the activities of nitrile hydratase and amidase and resistance of these enzymes to ammonium were observed in mutant of R. rhodichrous resistant to phosphotricine, an inhibitor of GS. An important role of GS in the mechanism of adaptation is suggested.
Astaurova
O B
OB
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Leonova
T E
TE
Poliakova
I N
IN
Sineokaia
I V
IV
Gordeev
V K
VK
Ianenko
A S
AS
D020106:Acrylamide; D000222:Adaptation, Physiological; D000581:Amidohydrolases / Q000502:physiology; D003470:Culture Media; D018086:Glutamate Dehydrogenase (NADP+) / Q000502:physiology; D005974:Glutamate-Ammonia Ligase / Q000502:physiology; D006836:Hydro-Lyases / Q000502:physiology; D000644:Quaternary Ammonium Compounds; D012240:Rhodococcus / Q000502:physiology*
D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D020106:Acrylamide; D018086:Glutamate Dehydrogenase (NADP+); D000581:Amidohydrolases; C019665:amidase; D006836:Hydro-Lyases; C048831:nitrile hydratase; D005974:Glutamate-Ammonia Ligase