Separation of D-lysergic acid diethylamide derivatives using micellar electrokinetic capillary chromatography.
By adjusting column temperature and applied electric field, a fast separation in micellar electrokinetic capillary chromatography was developed for the separation of D-lysergic acid diethylamide derivatives. A baseline separation of nine derivatives was accomplished with a run time of less than 12 min by utilizing elevated column temperature (60 degrees C) and an applied electric field of 387 V/cm. The number of plates generated per unit time for the separations completed at elevated temperatures was significantly higher when compared to separations at the same applied electric field but at lower temperatures (20 degrees C).
Djordjevic
M N
MN
Novartis Pharma Inc., Pharma Discovery, Basel, Switzerland. nebojsa.djordjevic@pharma.novartis.com
Fitzpatrick
F
F
Houdiere
F
F
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation / Q000379:methods*; D008238:Lysergic Acid Diethylamide / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D013329:Structure-Activity Relationship
D008238:Lysergic Acid Diethylamide