CD30+ T cells in rheumatoid synovitis: mechanisms of recruitment and functional role.
High serum levels of soluble CD30 (sCD30) have been reported to better predict the response to second line therapy in rheumatoid arthritis (RA). It is believed that sCD30 is released by CD30+ T cells present in the RA synovium. However, both the mechanism of recruitment to the joint and the functional role of this T cell subset in the pathogenesis of the disease remain unknown. This study confirmed higher levels of sCD30 in the serum and synovial fluid (SF) of RA patients compared with normal controls. However, analysis of mRNA and cell surface CD30 expression showed that CD30+ T cells are detectable in the SF, but not in the synovial membrane. In contrast, T cells expressing the CD30 transcript, but not the surface molecule, were found in the peripheral blood of both RA and normal controls. CD30 surface expression was up-regulated by adhesion and migration through endothelium in vitro and in a delayed-type hypersensitivity model in vivo. Although the great majority of fresh or cloned CD30+ T cells from SF produced both IFN-gamma and IL-4, CD30 expression strictly correlated with IL-4 synthesis in synovial T cell clones. In addition, CD30+ T cell clones also produced high amounts of the anti-inflammatory cytokine IL-10. On this basis, we would like to propose that synovial CD30+ cells may play a role in the control of the inflammatory response. Serum sCD30 may reflect such cell activity and, therefore, explain the previously demonstrated correlation between high sCD30 serum levels and positive response to therapy.
Gerli
R
R
Department of Clinical and Experimental Medicine, Section of Internal Medicine and Oncological Sciences, Center for the Study of Rheumatic Diseases, University of Perugia, Perugia, Italy.
Pitzalis
C
C
Bistoni
O
O
Falini
B
B
Costantini
V
V
Russano
A
A
Lunardi
C
C
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology*; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D003593:Cytoplasm / Q000276:immunology / Q000378:metabolism; D005260:Female; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D017730:Ki-1 Antigen / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis; D012995:Solubility; D013585:Synovitis / Q000097:blood / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D016207:Cytokines; D006684:HLA-DR Antigens; D017730:Ki-1 Antigen; D037181:Lectins, C-Type; D017493:Leukocyte Common Antigens; D012333:RNA, Messenger; D000099192:CD69 Antigens