A new model of chronic cardiac ischemia in rabbits.
Chronic cardiac ischemia has mainly been studied in large species such as pigs or dogs. Little research has been performed using small species such as rabbits. In the present study, 1-3 wk after implantation of a novel device (ameroid) on the circumflex coronary artery of New Zealand White rabbits, vessel patency was evaluated by coronary angiography, corrosion cast, and radiolabeled microspheres. Coronary angiograms showed, after 21 days, either total occlusion or severe stenosis in seven of eight arteries, which was confirmed by corrosion casts. The ameroid group had less blood flow in the epicardial (-62%) and endocardial (-54%) layers of the ischemic area compared with sham-operated rabbits (P < 0.05). Blood flow increased in the ischemic area compared with day 0 during acute occlusion, suggesting that progressive coronary occlusion initiated the growth of de novo collateral vessels. Thus we have developed a new model of chronic cardiac ischemia in rabbits with documented progressive coronary stenosis and occlusion that is suitable to test various therapeutic angiogenesis strategies.
Operschall
C
C
Pharma Division, Cardiovascular Preclinical Research, F. Hoffmann-La Roche Ltd., 4070 Basel, Switzerland. christine.operschall@roche.com
Falivene
L
L
Clozel
J P
JP
Roux
S
S
D000818:Animals; D001672:Biocompatible Materials; D001783:Blood Flow Velocity; D002364:Caseins; D017023:Coronary Angiography; D003326:Coronary Circulation / Q000502:physiology*; D003331:Coronary Vessels / Q000502:physiology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004285:Dogs; D020100:Hydrogels; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology*; D009206:Myocardium / Q000473:pathology; D019736:Prostheses and Implants; D011817:Rabbits; D013552:Swine
D001672:Biocompatible Materials; D002364:Caseins; D020100:Hydrogels; C009719:ameroid