Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen.
A clinical assay to evaluate total acrosin activity developed for human semen has been optimized for use in boar spermatozoa. The main modifications included a decrease of sperm number per assay from 1.0 to 10.0 x 10(6) to 12.5 to 75.0 x 10(3) spermatozoa, and the time of incubation from 180 to 60 min. Linearity of response for differing quantities of spermatozoa was maintained. Extensive washing of spermatozoa was necessary to eliminate seminal plasma, the source of acrosin inhibitors. Seminal plasma that was diluted 1000 times inhibited acrosin activity by about 50%. To abolish the inhibitory effect of seminal plasma it was necessary to use 25,000-fold dilution. Total acrosin activity of boar spermatozoa was about 100 times higher than that of human spermatozoa. Acrosin activity of boar spermatozoa in extended semen decreased during 7 d of storage. These results indicate that the clinical assay of acrosin activity can be used for boar spermatozoa to evaluate the quality of boar semen.
Glogowski
J
J
Department of Molecular Andrology, Polish Academy of Sciences, Olsztyn, Poland.
Demianowicz
W
W
Piros
B
B
Ciereszko
A
A
D000176:Acrosin / Q000378:metabolism*; D000818:Animals; D008297:Male; D017830:Octoxynol / Q000494:pharmacology; D012661:Semen / Q000201:enzymology*; D012662:Semen Preservation; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000201:enzymology; D013552:Swine / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D017830:Octoxynol; D000176:Acrosin