Cytotoxicity in pig hepatocytes induced by 8-quinolinol, chloramine-T and natamycin.
The potential cytotoxic effects of the compounds 8-quinolinol, chloramine-T and natamycin have been studied in isolated pig hepatocytes. The relative cytotoxicity of these compounds was evaluated on the basis of the leakage of cytosolic lactate dehydrogenase (LDH), 3-(4,5 dimethyl)thiazol-2-yl,-2,5-diphenyl tetrazolium bromide (MTT) reduction by mitochondrial dehydrogenases, uptake of neutral red (NR) by cytosolic lysosomes, glutathion (GSH) depletion and oxidized glutathion (GSSG) efflux after 24 h exposure. Evaluation of the 20%, 50% and 80% reduced absorbance data obtained from the parameters NR20, NR50, and NR80, and MTT20, MTT50 and MTT80 enabled us to rank these compounds in decreasing order of cytotoxicity: 8-quinolinol > natamycin > chloramine-T. Also for the parameters LDH and GSH, chloramine-T appears to be less cytotoxic than natamycin and 8-quinolinol. Our study demonstrated that pig hepatocytes may be a useful model for examining cytotoxic events of drugs to be used in pigs, therefore avoiding possible extrapolation problems due to species differences.
Martínez-Larrañaga
M R
MR
Department of Toxicology and Pharmacology, Faculty of Veterinary Medicine, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Anadón
A
A
Fernandez-Cruz
M L
ML
Díaz
M J
MJ
Martinez
M A
MA
Frejo
M T
MT
Martínez
M
M
Tafur
M
M
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000633:toxicity*; D002478:Cells, Cultured; D002700:Chloramines / Q000633:toxicity*; D008099:Liver / Q000166:cytology; D008928:Mitochondria / Q000187:drug effects* / Q000502:physiology*; D010866:Natamycin / Q000633:toxicity*; D015125:Oxyquinoline / Q000633:toxicity*; D013552:Swine; D014105:Tosyl Compounds / Q000633:toxicity*
D000891:Anti-Infective Agents, Local; D002700:Chloramines; D014105:Tosyl Compounds; C016300:chloramine-T; D015125:Oxyquinoline; D010866:Natamycin