Modification of disaccharidase activity measurement in rat bowel.
This study describes a modification of the existing disaccharidase assay in rat small bowel in which whole bowel, rather than mucosa, is utilized. In addition, the use of total vs. specific activity as a more accurate unit of measurement of disaccharidase activity is discussed.
Arcuni
J
J
Department of Surgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, USA.
Wang
L
L
Franson
R
R
Sonnino
R E
RE
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004186:Disaccharidases / Q000032:analysis / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000502:physiology; D007421:Intestine, Small / Q000201:enzymology* / Q000502:physiology; D043322:Lactase; D008297:Male; D009130:Muscle, Smooth / Q000201:enzymology / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D015203:Reproducibility of Results; D013393:Sucrase / Q000032:analysis / Q000378:metabolism; D000520:alpha-Glucosidases / Q000032:analysis / Q000378:metabolism; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism
D015415:Biomarkers; D004186:Disaccharidases; D043322:Lactase; D000520:alpha-Glucosidases; D001616:beta-Galactosidase; D013393:Sucrase