[Factors influencing transperitoneal transport of glucose in vitro].
The effect of mixing fluid intensification, damage of mesothelial cells and gentamicin on the diffusive glucose transport across the peritoneal membrane were evaluated in the presented in vitro studies. A mathematical model of the mass transport was used to calculate the diffusive permeability, expressed as a diffusive permeability coefficient P [cm x s-1], for the investigated specimens. In the control conditions glucose transfer from the interstitial to the mesothelial side of membrane (I-->M) and in the opposite direction (M-->I) remained constant and P value at mean was 2.731 +/- 1.493 [cm x s-1 x 10(-4)]. The change of the stirring rate from 5.5 to 11 mL/min increased P values by about 74% for transport direction I-->M and 58% for M-->I, but change from 11 to 22 mL/min enhanced P at mean by about 42% for the both direction. The damage of the mesothelial layer, using of sodium deoxycholate (2.5 mmol/L; 103.6 mg%), increased the glucose transfer from the interstitial to the mesothelial side of the peritoneum by 41% and to the opposite direction by 57%. Furthermore, gentamicin did not change the I-->M transfer, but diminished M-->I transport by about 12%.


CONCLUSIONS
1. The reducing of unstirred fluid layer at the mesothelium and interstitium--fluid interface and the mesothelium damage increase the diffusive glucose transport in vitro; 2. The unstirred fluid layer restricts glucose transfer more than tissue barrier (mesothelium); 3. The peritoneal glucose transport, directed from the mesothelial to the interstitial side of the peritoneum (but not transfer to the opposite direction), decreases slightly after gentamicin introduction.
Czyzewska
K
K
Katedra i Zakład Biochemii Klinicznej AM im. Karola Marcinkowskiego w Poznaniu.
Szary
B
B
Waniewski
J
J
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003840:Deoxycholic Acid / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D008954:Models, Biological; D010537:Peritoneum / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D011817:Rabbits
D005839:Gentamicins; D003840:Deoxycholic Acid; D005947:Glucose