title: A whole-genome assembly of Drosophila.
abstract: We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99. 99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.
authors.affiliation: Celera Genomics, Inc., 45 West Gude Drive, Rockville, MD 20850, USA. Gene.Myers@celera.com
authors.forename: E W
authors.initials: EW
authors.lastname: Myers
authors.forename: G G
authors.initials: GG
authors.lastname: Sutton
authors.forename: A L
authors.initials: AL
authors.lastname: Delcher
authors.forename: I M
authors.initials: IM
authors.lastname: Dew
authors.forename: D P
authors.initials: DP
authors.lastname: Fasulo
authors.forename: M J
authors.initials: MJ
authors.lastname: Flanigan
authors.forename: S A
authors.initials: SA
authors.lastname: Kravitz
authors.forename: C M
authors.initials: CM
authors.lastname: Mobarry
authors.forename: K H
authors.initials: KH
authors.lastname: Reinert
authors.forename: K A
authors.initials: KA
authors.lastname: Remington
authors.forename: E L
authors.initials: EL
authors.lastname: Anson
authors.forename: R A
authors.initials: RA
authors.lastname: Bolanos
authors.forename: H H
authors.initials: HH
authors.lastname: Chou
authors.forename: C M
authors.initials: CM
authors.lastname: Jordan
authors.forename: A L
authors.initials: AL
authors.lastname: Halpern
authors.forename: S
authors.initials: S
authors.lastname: Lonardi
authors.forename: E M
authors.initials: EM
authors.lastname: Beasley
authors.forename: R C
authors.initials: RC
authors.lastname: Brandon
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: P J
authors.initials: PJ
authors.lastname: Dunn
authors.forename: Z
authors.initials: Z
authors.lastname: Lai
authors.forename: Y
authors.initials: Y
authors.lastname: Liang
authors.forename: D R
authors.initials: DR
authors.lastname: Nusskern
authors.forename: M
authors.initials: M
authors.lastname: Zhan
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Zheng
authors.forename: G M
authors.initials: GM
authors.lastname: Rubin
authors.forename: M D
authors.initials: MD
authors.lastname: Adams
authors.forename: J C
authors.initials: JC
authors.lastname: Venter
mesh_terms: D000465:Algorithms; D000818:Animals; D002843:Chromatin / Q000235:genetics; D019295:Computational Biology; D020451:Contig Mapping; D004331:Drosophila melanogaster / Q000235:genetics*; D022041:Euchromatin; D017344:Genes, Insect; D016678:Genome; D006570:Heterochromatin / Q000235:genetics; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites
chemical_list: D002843:Chromatin; D022041:Euchromatin; D006570:Heterochromatin