The structure of the cemento-dentinal junction in rat molars.
The cemento-dentinal junction was observed in the acellular and cellular cementum of rat molars by light and scanning electron microscopy. Scanning electron microscopy, combined with NaOH maceration, was used to observe the fibrous architecture directly in this region. Light microscopy revealed that the cemento-dentinal junction contains fewer collagen fibrils and more proteoglycans than the cementum and dentin. Scanning electron microscopy also showed that fibril intermingling is found only in some regions of the fibril-poor junction in macerated specimens. Prolonged maceration breaks down the cemento-dentinal junction in spite of the fibril intermingling. Only macerated specimens showed detachment here. It was established that NaOH maceration removes interfibrillar substances effectively, and does not damage the fibril structure or architecture. This suggests that the adhesion of proteoglycans is more important than fibril intermingling for preserving the cemento-dentinal attachment in the rat molar.
Yamamoto
T
T
Department of Oral Anatomy II, Hokkaido University School of Dentistry, Sapporo, Japan.
Domon
T
T
Takahashi
S
S
Islam
N M
NM
Suzuki
R
R
Wakita
M
M
D000818:Animals; D003094:Collagen / Q000032:analysis; D003739:Dental Cementum / Q000166:cytology* / Q000648:ultrastructure; D003804:Dentin / Q000166:cytology* / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000648:ultrastructure*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012972:Sodium Hydroxide
D012972:Sodium Hydroxide; D003094:Collagen