title: HIV-1 subtype C in commerical sex workers in Addis Ababa, Ethiopia.
abstract: In this study, we have investigated the diversity of the current HIV-1 strains circulating in Addis Ababa, Ethiopia; in addition, we have evaluated the applicability of peptide enzyme-linked immunosorbent assay (ELISA) and heteroduplex mobility assay (HMA) for HIV-1 subtyping. Previous studies have indicated that HIV-1 subtype C is the major subtype present in HIV-positive samples collected from various risk groups between 1988 and 1995 in Addis Ababa. To assess the possible influx of new HIV-1 subtypes, 150 commercial sex workers (CSW) reporting in 1997 to two Health Centers in Addis Ababa were enrolled in an unlinked anonymous cross-sectional study. Subtyping was performed according to the World Health Organization algorithm of peptide ELISA, followed by HMA and DNA sequencing. As a result, the HIV-1 prevalence among these CSWs was found to be 45% (67 of 150). Of the 67 samples, 66 contained HIV-1 of subtype C and only one was of subtype D. This confirms the persistent overall presence of HIV-1 subtype C in Addis Ababa and a low influx of other subtypes into this location.
authors.affiliation: Ethiopian Health and Nutrition Research Institute, Addis Ababa. ENARP@telecom.net.et
authors.forename: M
authors.initials: M
authors.lastname: Hussein
authors.forename: A
authors.initials: A
authors.lastname: Abebe
authors.forename: G
authors.initials: G
authors.lastname: Pollakis
authors.forename: M
authors.initials: M
authors.lastname: Brouwer
authors.forename: B
authors.initials: B
authors.lastname: Petros
authors.forename: A L
authors.initials: AL
authors.lastname: Fontanet
authors.forename: T F
authors.initials: TF
authors.lastname: Rinke de Wit
mesh_terms: D000595:Amino Acid Sequence; D004279:DNA, Viral / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005002:Ethiopia / Q000453:epidemiology; D015699:HIV Envelope Protein gp120 / Q000032:analysis; D015528:HIV Seroprevalence; D015497:HIV-1 / Q000145:classification* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D011477:Sex Work
chemical_list: D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments