title: Targeted insertion of a lacZ reporter gene into the mouse Cer1 locus reveals complex and dynamic expression during embryogenesis.
abstract: The mouse Cer1 (mCer1, Cer-l, Cerr1) gene encodes one member of a family of cytokines structurally and functionally related to the Xenopus head-inducing factor, Cerberus (xCer). We generated a mouse line in which the Cer1 gene was inactivated by replacing the first coding exon with a lacZ reporter gene. Mice homozygous for this allele (Cer1(lacZ)) showed no apparent perturbation of embryogenesis or later development. However, the lacZ reporter revealed a number of hitherto uncharacterised sites of Cer1 expression in late fetal and adult tissues. Preliminary analysis suggests that Cer1 is not essential for their morphogenesis, differentiation, or homeostasis.
authors.forename: E G
authors.initials: EG
authors.lastname: Stanley
authors.forename: C
authors.initials: C
authors.lastname: Biben
authors.forename: J
authors.initials: J
authors.lastname: Allison
authors.forename: L
authors.initials: L
authors.lastname: Hartley
authors.forename: I P
authors.initials: IP
authors.lastname: Wicks
authors.forename: I K
authors.initials: IK
authors.lastname: Campbell
authors.forename: M
authors.initials: M
authors.lastname: McKinley
authors.forename: L
authors.initials: L
authors.lastname: Barnett
authors.forename: F
authors.initials: F
authors.lastname: Koentgen
authors.forename: L
authors.initials: L
authors.lastname: Robb
authors.forename: R P
authors.initials: RP
authors.lastname: Harvey
mesh_terms: D000818:Animals; D029681:Arabidopsis Proteins; D005314:Embryonic and Fetal Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D010940:Plant Proteins / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; C101547:CER protein, Arabidopsis; D010940:Plant Proteins