Development and use of monoclonal antibodies to chicken fibronectin to show that the chicken hepatocellular carcinoma cell line, LMH, constitutively expresses fibronectin.
Fibronectin (Fn) is a high molecular weight glycoprotein and acute phase reactant that contributes to a variety of cellular activities including proliferation and wound healing. Production of Fn is influenced by cytokines such as IL-1alpha, IL-6 and TNF -alpha, and in serum Fn levels can function as an indicator of sepsis and reticulo-endothelial function. Here we describe the production of a panel of mAb to chicken Fn and give evidence that a chicken hepatocellular carcinoma cell line, LMH, constitutively expresses Fn. A capture ELISA to measure chicken Fn was developed using an IgG1 mAb (AV62) as the capture Ab, and biotinylated AV63 (IgG2b) as the detecting Ab. This study identified a single commercially available mAb directed against human Fn that also recognised chicken Fn. By contrast, the anti-chicken Fn mAbs did not cross-react with either human or bovine Fn.
Lynagh
G R
GR
Institute for Animal Health, Compton, Berkshire, RG20 7NN, UK.
Collins
R A
RA
Kaiser
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002642:Chick Embryo; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000276:immunology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000276:immunology*; D051379:Mice; D011201:Poultry Diseases / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D015850:Interleukin-6