title: Prokaryotic diversity in Zostera noltii-colonized marine sediments.
abstract: The diversity of microorganisms present in a sediment colonized by the phanerogam Zostera noltii has been analyzed. Microbial DNA was extracted and used for constructing two 16S rDNA clone libraries for Bacteria and Archaea. Bacterial diversity was very high in these samples, since 57 different sequences were found among the 60 clones analyzed. Eight major lineages of the Domain Bacteria were represented in the library. The most frequently retrieved bacterial group (36% of the clones) was delta-Proteobacteria related to sulfate-reducing bacteria. The second most abundant group (27%) was gamma-Proteobacteria, including five clones closely related to S-oxidizing endosymbionts. The archaeal clone library included members of Crenarchaeota and Euryarchaeota, with nine different sequences among the 15 analyzed clones, indicating less diversity when compared to the Bacteria organisms. None of these sequences was closely related to cultured Archaea organisms.
authors.affiliation: División de Microbiología, Universidad Miguel Hernández, Campus de San Juan, 03550 San Juan, Alicante, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Cifuentes
authors.forename: J
authors.initials: J
authors.lastname: Antón
authors.forename: S
authors.initials: S
authors.lastname: Benlloch
authors.forename: A
authors.initials: A
authors.lastname: Donnelly
authors.forename: R A
authors.initials: RA
authors.lastname: Herbert
authors.forename: F
authors.initials: F
authors.lastname: Rodríguez-Valera
mesh_terms: D001105:Archaea / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003001:Cloning, Molecular; D019641:DNA, Archaeal / Q000032:analysis / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D006109:Poaceae / Q000254:growth & development*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S