title: Cloning and sequence determination of the chloroplast psbA gene in Magnolia pyramidata (Magnoliales; Magnoliaceae).
abstract: The full length gene encoding the D1 protein of photosynthesis (psbA) has been cloned and sequenced from Magnolia pyramidata (Magnoliaceae). Despite considerable investigation into psbA structure and function in many algal lineages and a few agricultural plants, there has been little effort invested toward characterizing a broader range of plant psbA genes. This is the first report of a psbA gene sequence from a primitive angiosperm. The DNA and deduced amino acid sequences maintain high overall conservation with other taxa, suggesting a role for psbA in broad based angiosperm phylogenetic reconstruction.
authors.affiliation: Tulane University, Department of Cell and Molecular Biology, New Orleans, LA 70118-5698, USA. djobes@mailhost.tcs.tulane.edu
authors.forename: D V
authors.initials: DV
authors.lastname: Jobes
authors.forename: D L
authors.initials: DL
authors.lastname: Hurley
authors.forename: L B
authors.initials: LB
authors.lastname: Thien
mesh_terms: D003001:Cloning, Molecular; D017343:Genes, Plant / Q000235:genetics; D019684:Magnoliopsida / Q000235:genetics*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase; D017385:Sequence Homology
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D012337:RNA, Ribosomal, 18S; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase