title: Cytotoxic activity of flavonoids and extracts from Retama sphaerocarpa Boissier.
abstract: Seven flavonoids isolated from chloroform, ethyl acetate and butanol extracts, obtained from the aerial parts of Retama sphaerocarpa, have been assessed for cytotoxic activity against three human cancer cell lines: TK-10 (renal adenocarcinoma), MCF-7 (breast adenocarcinoma) and UACC-62 (melanoma), using the SRB assay. All of them, extracts and flavonoids, were actives in, at least, one of the three cell lines at the recommended National Cancer Institute doses. They produce a dose-dependent inhibition of cell growth at concentrations in the 10(-6)-10(-4) M and 25-250 microg/ml range for the flavonoids and extracts respectively, being the flavonol rhamnazin the most cytotoxic.
authors.affiliation: Departamento de Farmacología, Falcultad de Farmacia, Universidad de Sevilla, España.
authors.forename: M
authors.initials: M
authors.lastname: López-Lázaro
authors.forename: C
authors.initials: C
authors.lastname: Martín-Cordero
authors.forename: F
authors.initials: F
authors.lastname: Cortés
authors.forename: J
authors.initials: J
authors.lastname: Piñero
authors.forename: M J
authors.initials: MJ
authors.lastname: Ayuso
mesh_terms: D000230:Adenocarcinoma; D001943:Breast Neoplasms; D002240:Carbohydrate Sequence; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000633:toxicity*; D044948:Flavonols / Q000737:chemistry / Q000633:toxicity*; D006027:Glycosides / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D007680:Kidney Neoplasms; D019684:Magnoliopsida / Q000737:chemistry*; D008545:Melanoma; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000633:toxicity*; D014407:Tumor Cells, Cultured
chemical_list: D005419:Flavonoids; D044948:Flavonols; D006027:Glycosides; D010936:Plant Extracts; C000597862:rhamnazin