title: Identification of a polymorphic ryanodine receptor gene from Heliothis virescens (Lepidoptera: noctuidae).
abstract: cDNAs encoding the C-terminal 1172 amino acids of a ryanodine receptor (RyR) from the lepidopteran pest Heliothis virescens (Hv-RyR) have been cloned and characterised. Sequence comparisons, organisational studies on corresponding genomic regions and a genetic segregation analysis provide evidence for two polymorphic alleles of the Hv-RyR locus. Comparison of the Hv-RyR C-terminal amino acid sequence with equivalent regions of other RyRs reveals a high level of overall amino acid homology (74% identity with D. melanogaster and between 47.9 and 50.1% with vertebrate isoforms). Homologies are however not uniformly distributed, though regions of high and low similarity are consistent with patterns in other RyR isoforms. The structural similarity of Hv-RyR with other RyRs is also indicated by comparison of hydropathy profiles and other previously described functional domains. Such results are consistent with this region of Hv-RyR containing the Ca(2+) channel itself and being intimately involved in RyR regulation. Potential uses of the cDNAs described in the discovery and development of novel ryanodine like insecticides are discussed.
authors.affiliation: Jealott's Hill Research Station, Zeneca Agrochemicals, Bracknell, UK.
authors.forename: E
authors.initials: E
authors.lastname: Puente
authors.forename: M
authors.initials: M
authors.lastname: Suner
authors.forename: A D
authors.initials: AD
authors.lastname: Evans
authors.forename: A R
authors.initials: AR
authors.lastname: McCaffery
authors.forename: J D
authors.initials: JD
authors.lastname: Windass
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D011110:Polymorphism, Genetic; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D019837:Ryanodine Receptor Calcium Release Channel