RAPD polymorphisms detected among the flax genotrophs.
The occurrence of environmentally induced heritable changes in certain flax varieties has been shown to be accompanied by changes in the genomic DNA. A large difference in nuclear DNA contents has been characterized between the extreme types, termed genotrophs. The genomic variation between a series of genotrophs has been studied by the polymerase chain reaction using random arbitrary oligonucleotide primers. A total of 320 primers were used in the reactions and 253 polymorphic bands observed. The polymorphic bands were derived from all parts of the genome, namely the highly repetitive. middle-repetitive and low-copy-number sequences. They were also shown to be distributed throughout the genome. In one group of genotrophs, all of which were induced by temperature treatment, there was a clustering of the polymorphisms with a high degree of shared polymorphisms. These results are in agreement with earlier studies showing that a dispersed fraction of the genome is susceptible to variation when environmentally induced heritable changes occur.
Cullis
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CA
Department of Biology, Case Western Reserve University, Cleveland, OH 44106-7080, USA.
Swami
S
S
Song
Y
Y
D018744:DNA, Plant / Q000235:genetics*; D019597:Flax / Q000235:genetics*; D014644:Genetic Variation; D018745:Genome, Plant; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique
D018744:DNA, Plant