title: [Optimization of conditions for cultivation of the basidiomycete Coriolus hirsutus--producer of extracellular laccase].
abstract: The effects of various factors on the biosynthesis of extracellular laccase (EC 1.14.18.1) by the basidiomycete Coriolus hirsutus (Wulf.: Fr.) Quel. no. 072 during submerged cultivation were examined. Optimal parameters for cultivation in a fermenter of 10 l were determined: temperature, 28 degrees C; stirrer rotation speed, 160 rpm; and the inoculum volume, 15% of the working volume of the fermenter. The filtrate contained peroxidase, laccase, and phenol oxidase activities and displayed a high thermal stability.
authors.affiliation: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Koroleva
authors.forename: E V
authors.initials: EV
authors.lastname: Stepanova
authors.forename: V P
authors.initials: VP
authors.lastname: Gavrilova
authors.forename: N S
authors.initials: NS
authors.lastname: Iakovleva
authors.forename: E O
authors.initials: EO
authors.lastname: Landesman
authors.forename: A I
authors.initials: AI
authors.lastname: Iaropolov
mesh_terms: D001487:Basidiomycota / Q000166:cytology / Q000378:metabolism*; D003470:Culture Media; D042845:Laccase; D010088:Oxidoreductases / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D010088:Oxidoreductases; D042845:Laccase