title: The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells.
abstract: The 56-kDa B1 subunit of the vacuolar H(+)ATPase has a C-terminal DTAL amino acid motif typical of PDZ-binding proteins that associate with the PDZ protein, NHE-RF (Na(+)/H(+) exchanger regulatory factor). This B1 isoform is amplified in renal intercalated cells, which play a role in distal urinary acid-base transport. In contrast, proximal tubules express the B2 isoform that lacks the C-terminal PDZ-binding motif. Both the B1 56-kDa subunit and the 31-kDa (E) subunit of the H(+)ATPase are pulled down by glutathione S-transferase NHE-RF bound to GSH-Sepharose beads. These subunits associate in vivo as part of the cytoplasmic V1 portion of the H(+)ATPase, and the E subunit was co-immunoprecipitated from rat kidney cytosol with NHE-RF antibodies. The interaction of H(+)ATPase subunits with NHE-RF was inhibited by a peptide derived from the C terminus of the B1 but not the B2 isoform. NHE-RF colocalized with H(+)ATPase in either the apical or the basolateral region of B-type intercalated cells, whereas NHE-RF staining was undetectable in A-intercalated cells. In proximal tubules, NHE-RF was located in the apical brush border. In contrast, H(+)ATPase was concentrated in a distinct membrane domain at the base of the brush border, from which NHE-RF was absent, consistent with the expression of the truncated B2 subunit isoform in this tubule segment. The colocalization of NHE-RF and H(+)ATPase in B- but not A-intercalated cells suggests a role in generating, maintaining, or modulating the variable H(+)ATPase polarity that characterizes the B-cell phenotype.
authors.affiliation: Renal Unit and Program in Membrane Biology and the Molecular Neurogenetics Unit, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA. sbreton@receptor.mgh.harvard.edu
authors.forename: S
authors.initials: S
authors.lastname: Breton
authors.forename: T
authors.initials: T
authors.lastname: Wiederhold
authors.forename: V
authors.initials: V
authors.lastname: Marshansky
authors.forename: N N
authors.initials: NN
authors.lastname: Nsumu
authors.forename: V
authors.initials: V
authors.lastname: Ramesh
authors.forename: D
authors.initials: D
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007150:Immunohistochemistry; D007672:Kidney Cortex / Q000737:chemistry* / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000032:analysis*; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017923:Sodium-Hydrogen Exchangers / Q000032:analysis*
chemical_list: D010750:Phosphoproteins; D017923:Sodium-Hydrogen Exchangers; C093376:sodium-hydrogen exchanger regulatory factor; D006180:Proton-Translocating ATPases