title: Proton translocation by cytochrome c oxidase: a rejoinder to recent criticism.
abstract: Ten years ago, intermediate reaction steps in the catalytic cycle of cytochrome c oxidase were titrated with phosphorylation potential in isolated mitochondria, and the results were interpreted as evidence for thermodynamic linkage of proton translocation exclusively to the oxidative reaction steps of the catalytic cycle [Wikström, M. (1989) Nature 338, 776-778]. Michel has recently argued that this work was flawed, and proposed a mechanism in which one of the four steps of proton translocation is linked to the reductive phase of the catalytic cycle [Michel, H. (1999) Biochemistry 38, 15129-15140]. Here, the original data are scrutinized and related to information that has accumulated since this work was published. The analysis shows that the main conclusions from this work still hold. Michel's mechanism of proton translocation is briefly discussed, and found to be at odds with some experimental observations.
authors.affiliation: Helsinki Bioenergetics Group, Department of Medical Chemistry, Institute of Biomedical Sciences and Biocentrum Helsinki, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Wikström
mesh_terms: D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008956:Models, Chemical; D010766:Phosphorylation; D017494:Proton Pumps / Q000737:chemistry / Q000378:metabolism; D011522:Protons
chemical_list: D017494:Proton Pumps; D011522:Protons; D003576:Electron Transport Complex IV