Catalytic decomposition of 3-chloroperoxybenzoic acid by immobilized catalase in a non-aqueous medium.
Catalytic activities of catalase (CAT) immobilized on graphite--GMZ, soot--"NORIT" and "PM-100" to mediate decomposition of 3-Cl-C6H4COOOH (3-CPBA) in acetonitrile have been investigated. Under these conditions, the kinetic parameters Km, k, Ea, Vmax, and Z0 were calculated. Conclusions on a probable mechanism of the catalytic process observed were drawn from the calculated values of deltaG*, deltaH*, and deltaS*. A quantitative UV-spectrophotometrical approach was used as the basic analytical tool. The electrochemical reduction of oxygen generated in enzyme catalysed 3-CPBA decomposition was examined with polarization curves method.
Horozova
E
E
Department of Physical and Theoretical Chemistry, University of Plovdiv, Bulgaria. horozova@argon.uni-plovdiv.bg
Dimcheva
N
N
Jordanova
Z
Z
D002374:Catalase / Q000378:metabolism*; D020134:Catalytic Domain; D002723:Chlorobenzoates / Q000493:pharmacokinetics*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D010408:Penicillium chrysogenum / Q000201:enzymology
D002723:Chlorobenzoates; D004800:Enzymes, Immobilized; D002374:Catalase; C000433:3-chloroperbenzoic acid