Psoriasis patients with basal cell carcinoma have more repair-mediated DNA strand-breaks after UVC damage in lymphocytes than psoriasis patients without basal cell carcinoma.
We have investigated the formation of strand-breaks following UVC irradiation in lymphocytes from psoriasis patients with or without basal cell carcinoma (BCC). Isolated lymphocytes were irradiated with UVC light at a dose of 3.6 J/m(2), and the level of DNA strand-breaks were measured 25 min after the irradiation by the alkaline comet assay. The generation of strand-breaks following UVC irradiation indicates DNA-repair-mediated incisions, as UVC light does not generate strand-breaks per se. We found that psoriasis patients with BCC had more DNA-repair incisions than non-cancer patients. The incision level correlated to two polymorphisms of the XPD gene. At present, it is not clear if the association is a primary effect that is related to differences of the XPD protein. Genes encoding for other repair proteins, namely XRCC1, ERCC1, and LIG1 are located close to the XPD gene, and it is possible that the association is due to a cosegregation with a polymorphism in one of these genes.
Møller
P
P
National Institute of Occupational Health, DK-2100, Copenhagen, Denmark. peter.moller@farmakol.ku.dk
Wallin
H
H
Dybdahl
M
M
Frentz
G
G
Nexø
B A
BA
D000483:Alleles; D002280:Basal Cell Carcinoma / Q000150:complications* / Q000235:genetics* / Q000473:pathology; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects*; D004265:DNA Helicases; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects; D004268:DNA-Binding Proteins; D004307:Dose-Response Relationship, Radiation; D005091:Exons / Q000235:genetics; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics; D011506:Proteins / Q000235:genetics; D011565:Psoriasis / Q000150:complications* / Q000235:genetics* / Q000473:pathology; D012878:Skin Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors; D014466:Ultraviolet Rays; D051759:Xeroderma Pigmentosum Group D Protein
D004268:DNA-Binding Proteins; D011506:Proteins; D014157:Transcription Factors; D004265:DNA Helicases; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human