An evaluation of hybridization kinetics in biosensors using a single-fractal analysis.
The diffusion-limited hybridization kinetics of analyte in solution to receptor immobilized on a biosensor or immunosensor surface is analysed within a fractal framework. The data may be analysed by a single-fractal analysis. This was indicated by the regression analysis provided by Sigmaplot [Sigmaplot Users Manual (1993) Jandel Scientific, San Rafael, CA]. It is of interest to note that the binding-rate coefficient and the fractal dimension both exhibit changes in the same and in the opposite directions for the single example presented in each case. The binding-rate coefficient(s) expressions developed as a function of the analyte (DNA) concentration in solution and the fractal dimension are of particular value, since they provide a means to better control biosensor or immunosensor performance and provide physical insights into the hybridization process.
Vontel
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Chemical Engineering Department, University of Mississippi, University, MS 38677-9740, USA.
Ramakrishnan
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Sadana
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D001483:Base Sequence; D015374:Biosensing Techniques / Q000706:statistics & numerical data*; D001709:Biotechnology; D017709:Fractals; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data
D009838:Oligodeoxyribonucleotides