[Effect of vitamin D3 on free-radical oxidation of lipids in low density lipoproteins in vitamin D deficiency].
The lipid free radical oxidation in low density lipoproteins was investigated on D-deficiency model in vivo. The processes of lipid free radical oxidative activation in low density lipoproteins at D-deficiency occurred. The chemiluminescence kinetic parameters: the maximum intensity of the first flash and inclination angle tangent of an ascending branch of the second flash grew at D-deficiency in comparison with control group (p < 0.02 and p < 0.05, respectively). At the same time, the vitamin D3 introduction to the experimental animals diet failed result statistically reliable inclination angle tangent of an ascending branch of the second flash was reduced (p < 0.02). Increase of the products reacting with thiobarbituric acid content in low density lipoproteins in D-deficiency conditions (p < 0.001) was found. Vitamin D3 introduction to the diet reduced quantity of products reacting with thiobarbituric acid in low density lipoproteins (p < 0.01). However, their level remained higher than for the control animals (p < 0.01) as established.
Kuz'menko
A I
AI
A.V. Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. akuzm@hotmail.com
Morozova
R P
RP
Nikolenko
I A
IA
Donchenko
G V
GV
D000818:Animals; D002762:Cholecalciferol / Q000494:pharmacology*; D005609:Free Radicals; D050356:Lipid Metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D014808:Vitamin D Deficiency / Q000378:metabolism*
D005609:Free Radicals; D008077:Lipoproteins, LDL; D002762:Cholecalciferol