[Low expression of nm23-H1 mRNA and its protein in breast cancer].
OBJECTIVE
To explore the expression of nm23-H1 mRNA and its protein in breast cancer and its correlation to the biologic behavior.


METHODS
RT-PCR and immunohistochemical method were employed to detect the expression of nm23-H1 mRNA and its protein in 58 breast cancer tissues.


RESULTS
Low level expression of nm23-H1 mRNA and protein was shown in 44.8% and 48.2% of examined cases respectively. The low expression of nm23-H1 mRNA was correlated, but not always parallel to the protein expression. The low level expression of nm23-H1 mRNA or the protein was significantly associated with lymphatic metastasis of the cancer.


CONCLUSIONS
Examination of nm23-H1 expression may be of significance in evaluating metastatic potentiality and prognosis of breast cancer.
Guo
W
W
Department of General Surgery, Dalian Central Hospital, Dalian, Liaoning, 116033 P.R.China.
Wang
Y
Y
Zhuang
X
X
Bai
Y
Y
Zhang
J
J
D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis / Q000235:genetics*; D054778:NM23 Nucleoside Diphosphate Kinases; D009362:Neoplasm Metastasis; D009701:Nucleoside-Diphosphate Kinase; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*
D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins