Aggregated forms of heparin-binding and non-heparin-binding proteins of boar seminal plasma and their binding properties.
Boar seminal plasma proteins were separated by affinity chromatography on immobilized heparin into two portions: heparin-binding (H+) and non-heparin-binding (H-) proteins. Gel chromatography of the H+ portion yielded four main protein fractions of >150, 45, 30 and 20 kDa, while that of the H- portion resulted in the separation into three main protein fractions of >150, 30 and 20 kDa. HPLC analysis and N-terminal sequencing used to characterize the composition of the protein fractions obtained by gel chromatography revealed that all consisted of low (12-16 kDa) molecular weight components: the H+ fraction consisted of DQH sperm protein, AQN and AWN spermadhesins whereas the H- fraction consisted of PSPI and PSPII spermadhesins. The high molecular weight values of fractions obtained by gel chromatography thus suggest that the proteins are present in boar seminal plasma in the form of aggregates. Interactions of individual boar seminal plasma proteins and their aggregates present in the H+ and H- fractions with acid polysaccharides were estimated.
Manásková
P
P
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
Mészárosová
A
A
Liberda
J
J
Voburka
Z
Z
Tichá
M
M
Jonáková
V
V
D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D016264:Dextran Sulfate / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012661:Semen / Q000737:chemistry*; D020539:Sequence Analysis, Protein; D013075:Sperm Capacitation; D013552:Swine / Q000378:metabolism*; D015044:Zona Pellucida / Q000378:metabolism
D046911:Macromolecular Substances; D011134:Polysaccharides; D011506:Proteins; D006820:Hyaluronic Acid; D006493:Heparin; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate; C007789:fucoidan