Fluorescent amplified-fragment length polymorphism genotyping of Salmonella enterica subsp. enterica serovars and comparison with pulsed-field gel electrophoresis typing.
We have performed the fluorescently labeled amplified-fragment length polymorphism (FAFLP) method on 97 strains of the food-borne pathogen Salmonella enterica subsp. enterica comprising seven different serovars using the restriction enzymes EcoRI and MseI. From the total FAFLP fingerprinted strains, 81 were compared with pulsed-field gel electrophoresis (PFGE) typing of the same strains. The FAFLP method showed a discriminatory power equal to that of PFGE. We report a fast, robust, and high-resolution adaptation of the AFLP assay for fingerprinting S. enterica subsp. enterica serovars with capillary electrophoresis that can be scaled to high throughput on automated analysis instruments.
Lindstedt
B A
BA
Department of Bacteriology, National Institute of Public Health, N-0403 Oslo, Norway. bjorn-arne.lindstedt@flokehelsa.no
Heir
E
E
Vardund
T
T
Kapperud
G
G
D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D005453:Fluorescence; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012478:Salmonella Food Poisoning / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics*