title: Immunocytochemistry and DNA-image cytometry in diagnostic effusion cytology. II. Diagnostic accuracy in equivocal smears.
abstract: To determine sensitivity and specificity of different antibodies for the immunocytochemical detection of malignant cells in diagnostically equivocal effusions in comparison with those achieved by DNA-image cytometry. 65 cytologically doubtful effusions of the serous cavities were stained with twelve antibodies. Furthermore, DNA-image cytometry was performed. Data were correlated with patient follow-up. Sensitivity of cellular staining of Ber-EP4 for the identification of malignant cells was 77.8%, specificity of absent staining for benign cells was 100%. Positive predictive value for the identification of malignant cells was 100%, negative value 65.5%. Sensitivity of DNA-aneuploidy for the identification of malignancy was 82.9%, specificity of DNA-non-aneuploidy for benignity 94.7%. The positive predictive value of DNA-aneuploidy for the occurrence of malignant cells was 96.7%. Negative predictive value of DNA-non-aneuploidy was 72.0%. Combining immunocytochemistry applying Ber-EP4 only and DNA-cytometry in equivocal effusions resulted in a sensitivity of 88.9% for the identification of malignant cells associated with a 95.0% specificity. Positive predictive value was 97.7%, the negative one 79.2%.
authors.affiliation: Institute of Cytopathology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Motherby
authors.forename: N
authors.initials: N
authors.lastname: Friedrichs
authors.forename: M
authors.initials: M
authors.lastname: Kube
authors.forename: B
authors.initials: B
authors.lastname: Nadjari
authors.forename: K
authors.initials: K
authors.lastname: Knops
authors.forename: A
authors.initials: A
authors.lastname: Donner
authors.forename: B
authors.initials: B
authors.lastname: Baschiera
authors.forename: P
authors.initials: P
authors.lastname: Dalquen
authors.forename: A
authors.initials: A
authors.lastname: Böcking
mesh_terms: D000782:Aneuploidy; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005500:Follow-Up Studies; D006338:Heart Neoplasms / Q000473:pathology; D006801:Humans; D019044:Image Cytometry / Q000379:methods* / Q000592:standards; D007150:Immunohistochemistry / Q000379:methods* / Q000592:standards; D008175:Lung Neoplasms / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis / Q000276:immunology; D010490:Pericardial Effusion / Q000473:pathology; D010534:Peritoneal Neoplasms / Q000473:pathology; D010996:Pleural Effusion / Q000473:pathology*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods / Q000592:standards
chemical_list: D004273:DNA, Neoplasm; D009363:Neoplasm Proteins