Effects of Fusobacterium necrophorum subspecies necrophorum on extracellular matrix of tissue-cultured bovine kidney cells.
The effects of Fusobacterium necrophorum subsp. necrophorum on the extracellular matrix were investigated. The toxic preparation from the culture induced reduction in the number of tissue-cultured bovine kidney cells. The exposed cells often manifested partial loss of cytoplasm and were morphologically irregular. Scanning electron microscopy demonstrated partial loss of the microvilli on the exposed cells and roughness of the cell surfaces. Finally, sodium dodecyl sulphate polyacrylamide gel electrophoresis profiles revealed complete degradation of bovine collagen type 1 after treatment with the toxic preparation. This degradation was inhibited by the addition of homologous antiserum. These findings indicate that the degradation may contribute to the establishment of the infection caused by F.n. subsp. necrophorum.
Okada
Y
Y
Laboratory of Animal Health, Tokyo University of Agriculture, Japan.
Kanoe
M
M
Okamoto
K
K
Sakamoto
K
K
Yaguchi
Y
Y
Watanabe
T
T
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism* / Q000633:toxicity; D002417:Cattle; D002470:Cell Survival; D003094:Collagen / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005109:Extracellular Matrix / Q000187:drug effects / Q000648:ultrastructure*; D005353:Fibronectins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005675:Fusobacterium necrophorum / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning
D001426:Bacterial Proteins; D003470:Culture Media; D005353:Fibronectins; D003094:Collagen