title: Cloning and sequencing of the maltohexaose-producing amylase gene of Klebsiella pneumoniae.
abstract: The molecular characterization of the maltohexaose-producing amylase gene of Klebsiella pneumoniae revealed an open reading frame in which 2,031 base pairs encode a protein of 677 amino acids with a calculated molecular weight of 75,921. The amylase gene had high similarities of 73.6% in DNA sequence and 79.3% in deduced amino acid sequence with the periplasmic alpha-amylase MalS gene of Escherichia coli.
authors.affiliation: Department of Biotechnology, National Institute of Agrobiological Resources, Ibaraki, Japan. momma@abr.affrc.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Momma
mesh_terms: D000595:Amino Acid Sequence; D000681:Amylases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D009844:Oligosaccharides; C016549:maltohexaose; D000681:Amylases