title: Processing of the Man(10)GlcNAc (M(10)) oligosaccharide by alpha-mannosidases from Candida albicans.
abstract: The hydrolysis of Man(10)GlcNAc (M(10)) by purified alpha-mannosidases and its further processing by a mixed membrane preparation from Candida albicans were studied. Incubation of the oligosaccharide with purified alpha-mannosidases I (E-I) or II (E-II) from C. albicans released 1 and 2 mol of mannose per mol of M(10), respectively. This treatment converted M(10) into an acceptor substrate of further mannose residues from GDP-Man as catalyzed by membrane-bound mannosyltransferases. Elongation of E-I- or E-II-trimmed M(10) yielded a low molecular mass product (14-17 mannose residues added), and in the case of E-II, a minor amount of an additional product of a higher molecular mass. Our results indicate that purified alpha-mannosidases participate in N-glycan processing in C. albicans.
authors.affiliation: Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad Autónoma de Guanajuato, Apartado postal 187, Guanajuato, Mexico.
authors.forename: A B
authors.initials: AB
authors.lastname: Vázquez-Reyna
authors.forename: R
authors.initials: R
authors.lastname: Balcázar-Orozco
authors.forename: C
authors.initials: C
authors.lastname: Calvo-Méndez
authors.forename: E
authors.initials: E
authors.lastname: López-Romero
authors.forename: A
authors.initials: A
authors.lastname: Flores-Carreón
mesh_terms: D002176:Candida albicans / Q000201:enzymology* / Q000254:growth & development; D006868:Hydrolysis; D008361:Mannosidases / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D009844:Oligosaccharides; C025363:mannoproteins; D008361:Mannosidases