title: Characterization of three capsular polysacharides produced by Burkholderia pseudomallei.
abstract: Three kinds of capsular polysaccharide (CP) were found to be produced by Burkholderia pseudomallei. When the bacterium was grown with the medium without glycerol, CP-1a and CP-1b were produced. CP-1a was mainly 1.4-linked glucan and CP-1b was identified as a polymer composed of galactose and 3-deoxy-D-manno-octulosonic acid, whose chemical structure was recently reported by other laboratories. When the bacterium was grown with the medium containing 5" glycerol. CP-2 was synthesized. CP-2 contained galactose, rhamnose, mannose, glucose and a uronic acid in a ratio of approximately 3:1:0.3:1:1. Methylation analysis of the purified polysaccharides demonstrated that the two acidic polysaccharides. CP-1b and CP-2 shared no common structure, indicating that CP-2 was an acidic capsular polysaccharide whose chemical characters were not reported previously.
authors.affiliation: Department of Bacteriology, Kitasato Institute, 5-9-1 Shirokane Mmato-ku, Tokyo, Japan kawahara-k@kitasato.or.jp
authors.forename: K
authors.initials: K
authors.lastname: Kawahara
authors.forename: S
authors.initials: S
authors.lastname: Dejsirilert
authors.forename: T
authors.initials: T
authors.lastname: Ezaki
mesh_terms: D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media; D005990:Glycerol / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial; D005990:Glycerol