Preparation of diagnostic monoclonal antibodies against two potyviruses.
Diagnostic monoclonal and polyclonal antibodies against bean yellow mosaic virus (BYMV) and plum pox virus (PPV) were prepared, characterized and used for detection of these viruses in infected plants by enzyme-linked immunosorbent assay (ELISA), immunoblot analysis and tissue print immunoblot assay (TPIBA).
Subr
Z
Z
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic. virusubr@savba.savba.sk
Matisová
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis; D002213:Capsid / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000276:immunology; D017801:Plum Pox Virus / Q000276:immunology*; D017800:Potyvirus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes