title: A feasibility study of salivary gland autograft transplantation for xerostomia.
abstract: BACKGROUND
  Radiation-induced xerostomia is a frequent sequela in patients treated for cancer of the head and neck. One strategy to treat xerostomia would be to relocate portions of salivary tissue to adjacent submucosal sites that lie outside the radiation portals such as the anterior oral vestibule. It is not known whether salivary tissue transplanted as an autogenous free graft can survive, function adequately, and not produce mucoceles.
  
  
  METHODS
  Salivary gland tissue from the parotid and submandibular glands of the Syrian hamster were transplanted into the submucosal layer of the cheek pouch. After 3 months of observation, looking at graft size, graft extrusion, ulceration, infection, and mucocele formation, the graft sites were harvested. The specimens then underwent pathologic analysis by hematoxylin and eosin staining, as well as immunohistochemical methods to determine positivity for cytokeratin, smooth muscle actin (SMA), and amylase.
  
  
  RESULTS
  Histologic analysis of tissue harvested from Syrian hamsters grafted into the cheek pouch demonstrated intact, viable, organized salivary gland tissue. Eighty percent of the animals in the submandibular group and 63% of the animals in the parotid group had at least 1 graft with viable salivary tissue without undue complications.
  
  
  CONCLUSIONS
  Salivary gland tissue can be transplanted successfully as free autogenous grafts in the Syrian hamster model. Further studies are needed to determine whether the grafts will subsequently become functional and whether growth can be biologically stimulated. This approach may be a useful strategy to protect salivary gland tissue in patients undergoing radiotherapy for head and neck cancer.
authors.affiliation: University of Tennessee Department of Otolaryngology-HNS, 956 Court Ave, Suite B226, Memphis, Tennessee 38163, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Greer
authors.forename: M
authors.initials: M
authors.lastname: Eltorky
authors.forename: K T
authors.initials: KT
authors.lastname: Robbins
mesh_terms: D000818:Animals; D006224:Cricetinae; D004195:Disease Models, Animal; D005240:Feasibility Studies; D006085:Graft Survival; D008647:Mesocricetus; D012469:Salivary Glands / Q000473:pathology / Q000637:transplantation*; D015996:Survival Rate; D016378:Tissue Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014987:Xerostomia / Q000601:surgery*