Expression of adhesion factors and degrading proteins in primary and secondary glioblastomas and their precursor tumors.
In tumor tissue specimens of 27 primary and 17 secondary glioblastomas and the precursor lesions, the immunohistochemical expression patterns of the membrane protein CD44s, the basal lamina proteins laminin, collagen IV, and fibronectin, the lectin galectin-3 recognizing tenascin and N-CAM as well as of the matrix-degrading enzymes matrix metalloproteinase MMP-2 and MMP-9, and cathepsin D were studied. Besides expression of basal lamina proteins in vessels, all glioblastomas and the precursor lesions showed strong immunoreactivity of CD44s, tenascin, galectin-3, and N-CAM which were restricted to solid tumor masses. Present in solid tumor areas, MMP-2, MMP-9 and cathepsin D were also strongly expressed by single tumors cells invading adjacent brain tissue at the infiltrative margin. Neither the expression pattern in primary and secondary glioblastomas nor in the precursor tumors revealed significant differences. There was also no intraindividual constant expression pattern during glioma progression or correlation with malignancy. Restricted expression of CD44s, galectin-3, tenascin and N-CAM in solid tumor masses seems to contribute to homotypic tumor cell adhesion while single tumor cells abolish this expression profile and acquire invasive activities by expression of cathepsin D, MMP-2 and MMP-9.
Tews
D S
DS
Division of Neuropathology, Johannes Gutenberg University Medical Center, Mainz, Germany. tews@mail.Uni-Mainz.de
Nissen
A
A
D000328:Adult; D000943:Antigens, Differentiation / Q000032:analysis; D001254:Astrocytoma / Q000473:pathology; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology*; D002402:Cathepsin D / Q000032:analysis; D003094:Collagen / Q000032:analysis; D005353:Fibronectins / Q000032:analysis; D037502:Galectin 3; D005909:Glioblastoma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D020782:Matrix Metalloproteinases / Q000032:analysis*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000201:enzymology / Q000473:pathology*; D019006:Neural Cell Adhesion Molecules / Q000032:analysis*
D000943:Antigens, Differentiation; D005353:Fibronectins; D037502:Galectin 3; D018960:Hyaluronan Receptors; D007797:Laminin; D019006:Neural Cell Adhesion Molecules; D003094:Collagen; D002402:Cathepsin D; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9