Alpha-glucosidase inhibitors from clove (Syzgium aromaticum).
The ellagitannins, casuarictin and eugeniin, were isolated as rat intestinal maltase inhibitors from methanol extracts of clove (Syzgium aromaticum). Eugeniin showed inhibitory activity with an IC50 value of 10(-3) M. A structure-activity relationship study among the isolates and their related compound, penta-O-galloyl-beta-D-glucose, indicates that an increasing number of galloyl units in the molecule might lead to an increase in the inhibitory activity. Eugeniin also inhibited maltase activity toward the human intestinal epithelial cell line, Caco-2.
Toda
M
M
Laboratory of Food Biochemistry, Division of Applied Bioscience, Research School of Agriculture, Hokkaido University, Sapporo, Japan.
Kawabata
J
J
Kasai
T
T
D000818:Animals; D018938:Caco-2 Cells; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D007422:Intestines / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D051381:Rats; D016339:Spectrometry, Mass, Fast Atom Bombardment; D017365:Spices / Q000032:analysis*; D013329:Structure-Activity Relationship
D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors