title: The cytoplasmic domain of the alpha1 integrin subunit influences stress fiber formation via the conserved GFFKR motif.
abstract: Integrins are heterodimeric transmembrane proteins that mediate substrate adhesion and migration but also the bidirectional transfer of information across the plasma membrane via their cytoplasmic domains. We addressed the question of whether the very short cytoplasmic tail of the alpha1 integrin subunit of alpha1beta1 integrin is required for alpha1beta1-specific adhesion, spreading, and migration. For this purpose we transfected the alpha1 integrin subunit and two cytoplasmically truncated alpha1 subunits into Chinese hamster ovary (CHO) cells. Elimination of the entire cytoplasmic domain of the alpha1 subunit does not affect adhesion but leads to inhibition of spreading and stress fiber formation. The defect in spreading could not be rescued by lysophosphatidic acid, which has been reported to stimulate actin stress fiber formation via Rho. Additionally, deletion of the entire cytoplasmic domain of the alpha1 subunit abolishes migration toward alpha1beta1-specific substrates. Migration and stress fiber formation are similar in CHO-alpha1 cells and CHO cells carrying an alpha1 subunit still containing the conserved GFFKR motif. So, the GFFKR motif of the alpha1 subunit is essential and sufficient for these processes.
authors.affiliation: Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, Berlin-Dahlem, D-14195, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Vossmeyer
authors.forename: C
authors.initials: C
authors.lastname: Kaufmann
authors.forename: K
authors.initials: K
authors.lastname: Löster
authors.forename: L
authors.initials: L
authors.lastname: Lucka
authors.forename: R
authors.initials: R
authors.lastname: Horstkorte
authors.forename: W
authors.initials: W
authors.lastname: Reutter
authors.forename: K
authors.initials: K
authors.lastname: Danker
mesh_terms: D000199:Actins / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D017124:Conserved Sequence; D006224:Cricetinae; D003593:Cytoplasm / Q000502:physiology; D019281:Dimerization; D039423:Integrin alpha1; D016023:Integrins / Q000737:chemistry / Q000502:physiology; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion; D014162:Transfection
chemical_list: D000199:Actins; D015703:Antigens, CD; D039423:Integrin alpha1; D016023:Integrins; D011994:Recombinant Proteins