title: A BAC-based physical map of the major autosomes of Drosophila melanogaster.
abstract: We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.
authors.affiliation: Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. hoskins@bdgp.lbl.gov
authors.forename: R A
authors.initials: RA
authors.lastname: Hoskins
authors.forename: C R
authors.initials: CR
authors.lastname: Nelson
authors.forename: B P
authors.initials: BP
authors.lastname: Berman
authors.forename: T R
authors.initials: TR
authors.lastname: Laverty
authors.forename: R A
authors.initials: RA
authors.lastname: George
authors.forename: L
authors.initials: L
authors.lastname: Ciesiolka
authors.forename: M
authors.initials: M
authors.lastname: Naeemuddin
authors.forename: A D
authors.initials: AD
authors.lastname: Arenson
authors.forename: J
authors.initials: J
authors.lastname: Durbin
authors.forename: R G
authors.initials: RG
authors.lastname: David
authors.forename: P E
authors.initials: PE
authors.lastname: Tabor
authors.forename: M R
authors.initials: MR
authors.lastname: Bailey
authors.forename: D R
authors.initials: DR
authors.lastname: DeShazo
authors.forename: J
authors.initials: J
authors.lastname: Catanese
authors.forename: A
authors.initials: A
authors.lastname: Mammoser
authors.forename: K
authors.initials: K
authors.lastname: Osoegawa
authors.forename: P J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: S E
authors.initials: SE
authors.lastname: Celniker
authors.forename: R A
authors.initials: RA
authors.lastname: Gibbs
authors.forename: G M
authors.initials: GM
authors.lastname: Rubin
authors.forename: S E
authors.initials: SE
authors.lastname: Scherer
mesh_terms: D000818:Animals; D002503:Centromere / Q000235:genetics; D002843:Chromatin / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D020451:Contig Mapping; D016172:DNA Fingerprinting; D004331:Drosophila melanogaster / Q000235:genetics*; D022041:Euchromatin; D015723:Gene Library; D017344:Genes, Insect; D005819:Genetic Markers; D005822:Genetic Vectors; D016678:Genome; D017403:In Situ Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D016324:Sequence Tagged Sites; D016615:Telomere / Q000235:genetics
chemical_list: D002843:Chromatin; D022041:Euchromatin; D005819:Genetic Markers