title: MAGPIE/EGRET annotation of the 2.9-Mb Drosophila melanogaster Adh region.
abstract: Our challenge in annotating the 2.91-Mb Adh region of the Drosophila melanogaster genome was to identify genetic and genomic features automatically, completely, and precisely within a 6-week period. To do so, we augmented the MAGPIE microbial genome annotation system to handle eukaryotic genomic sequence data. The new configuration required the integration of eukaryotic gene-finding tools and DNA repeat tools into the automatic data collection module. It also required us to define in MAGPIE new strategies to combine data about eukaryotic exon predictions with functional data to refine the exon predictions. At the heart of the resulting new eukaryotic genome annotation system is a reverse comparison of public protein and complementary DNA sequences against the input genome to identify missing exons and to refine exon boundaries. The software modules that add eukaryotic genome annotation capability to MAGPIE are available as EGRET (Eukaryotic Genome Rapid Evaluation Tool).
authors.affiliation: The Rockefeller University, Laboratory of Computational Genomics, New York, New York 10021, USA. gaasterl@genomes.rockefeller.edu
authors.forename: T
authors.initials: T
authors.lastname: Gaasterland
authors.forename: A
authors.initials: A
authors.lastname: Sczyrba
authors.forename: E
authors.initials: E
authors.lastname: Thomas
authors.forename: G
authors.initials: G
authors.lastname: Aytekin-Kurban
authors.forename: P
authors.initials: P
authors.lastname: Gordon
authors.forename: C W
authors.initials: CW
authors.lastname: Sensen
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000818:Animals; D015220:Calcium Channels / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D016208:Databases, Factual; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics*; D005057:Eukaryotic Cells / Q000737:chemistry / Q000502:physiology; D017344:Genes, Insect / Q000235:genetics; D012984:Software
chemical_list: D015220:Calcium Channels; D000426:Alcohol Dehydrogenase