title: Study on RNA structure by pyrene-labeled 2'-O-methyloligoribonucleotides.
abstract: Properties of 2'-O-methyloligoribonucleotides containing 2'-O-(1-pyrenylmethyl)uridine were investigated as the fluorescent probe to search the single strand regions on RNA secondary and tertiary structure. The pyrene-labeled 2'-O-methyloligoribonucleotide (OMUpy) showed remarkable increase of fluorescence intensity to 333-fold at 375 nm when hybridized with the complementary oligoribonucleotide. When OMUpy, complementary to loop or stem regions, was applied to E. coli 5S-rRNA, the fluorescence intensities were increased in a sequence specific manner. The difference of the fluorescence intensities corresponds to the higher-order structure of 5S-rRNA, suggesting that pyrene-labled 2'-O-methyloligoribonucleotide can be applicable to search single strand regions of RNA.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Iwase
authors.forename: A
authors.initials: A
authors.lastname: Mahara
authors.forename: K
authors.initials: K
authors.lastname: Yamana
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
authors.forename: A
authors.initials: A
authors.lastname: Murakami
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli; D005456:Fluorescent Dyes; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011721:Pyrenes; D012313:RNA / Q000737:chemistry*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009843:Oligoribonucleotides; D011721:Pyrenes; D012341:RNA, Ribosomal, 5S; D012313:RNA; C030984:pyrene