Cytokinesis in cells undergoing mitosis without genome replication.
Chinese hamster ovary cells can be forced to enter mitosis without prior DNA replication by treatment with hydroxyurea and caffeine. Cells treated in this way assemble a spindle that functions normally except that it does not accomplish anaphase spindle elongation (anaphase B). The chromatin detaches from the unreplicated kinetochores, which fragment, but establish microtubule attachments and migrate to the metaphase plate. Partitioning of the kinetochore fragments ensues on the normal schedule. Typical midbodies and cleavage furrows are established and daughter cells of equal size are produced. These results imply that intact chromosomes are not necessary for correct cleavage furrow placement but that kinetochores might be. Further, it is clear that cleavage furrow placement does not depend on anaphase spindle elongation.
Wise
D A
DA
Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA. daw1@ra.msstate.edu
D000818:Animals; D016466:CHO Cells; D002110:Caffeine / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology*; D002875:Chromosomes / Q000187:drug effects / Q000378:metabolism; D006224:Cricetinae; D004261:DNA Replication; D016678:Genome; D018386:Kinetochores / Q000187:drug effects / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism; D014404:Tubulin / Q000187:drug effects / Q000378:metabolism
D014404:Tubulin; D002110:Caffeine