Increased expression of adrenomedullin mRNA in the tissues of intraocular and orbital tumors.
OBJECTIVE
To explore the possible involvement of adrenomedullin in the pathophysiology of intraocular and orbital tumors.


METHODS
Competitive reverse transcription-polymerase chain reaction was used to determine adrenomedullin mRNA levels in the tissues from 40 consecutive patients (40 eyes) undergoing vitrectomy, orbital tissue biopsy, or enucleation for various ocular diseases, including intraocular (n = 4) and orbital (n = 3) tumors, proliferative vitreoretinopathy (n = 8), proliferative diabetic retinopathy (n = 8), age-related macular degeneration (n = 4), preretinal macular fibrosis (n = 9), and acute retinal necrosis (n = 4).


RESULTS
Adrenomedullin mRNA levels in the tissues obtained from patients with intraocular or orbital tumors were significantly higher than those of patients with proliferative vitreoretinopathy (P <.05), proliferative diabetic retinopathy (P <.05), preretinal macular fibrosis (P <.005), and acute retinal necrosis (P <.01).


CONCLUSIONS
Adrenomedullin may play a role in the pathophysiology of intraocular and orbital tumors.
Udono
T
T
Department of Molecular Biology and Applied Physiology, Tohoku University School of Medicine, Aoba-ku, Sendai, Miyagi, Japan.
Totsune
K
K
Takahashi
K
K
Abe
T
T
Sato
M
M
Shibahara
S
S
Tamai
M
M
D053607:Adrenomedullin; D002830:Choroid Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D009918:Orbital Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012164:Retinal Diseases / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014665:Vasodilator Agents / Q000378:metabolism*
D010455:Peptides; D012333:RNA, Messenger; D014665:Vasodilator Agents; D053607:Adrenomedullin