Lipid peroxidation status, somatic mutations and micronuclei in peripheral lymphocytes: a case observation on a possible interrelationship.
A controlled dietary study was conducted in healthy female volunteers and reported elsewhere [1]. In a subset of samples four different biomarkers were analyzed: plasma malondialdehyde (MDA) levels and urinary 8-isoprostaglandin-F(2alpha) were measured as markers for lipid peroxidation. The frequency of hprt (hypoxanthine guanine phosphoribosyl transferase) mutants and micronuclei in peripheral blood lymphocytes were analyzed as indicators of genotoxic effects. One of the ten individuals showed extremely high background levels in all of the four endpoints measured. This case observation raises the possibility that life style factors and dietary habits affect the level of DNA reactive lipid peroxidation products, which in turn increase mutagenic and cytogenetic effects. A possible association between these biomarkers, particularly in relation to dietary fat intake and antioxidant status, should now be studied in a larger trial.
Mayer
C
C
Division of Toxicology and Cancer Risk Factors, German Cancer Research Center, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany. c.mayer@dkfz-heidelberg.de
Schmezer
P
P
Freese
R
R
Mutanen
M
M
Hietanen
E
E
Obe
G
G
Basu
S
S
Bartsch
H
H
D000328:Adult; D015415:Biomarkers; D018752:Diet, Fat-Restricted; D015237:Dinoprost / Q000652:urine; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D015227:Lipid Peroxidation; D008214:Lymphocytes / Q000378:metabolism*; D008315:Malondialdehyde / Q000097:blood; D048629:Micronuclei, Chromosome-Defective / Q000378:metabolism*; D008875:Middle Aged; D009154:Mutation
D015415:Biomarkers; D005231:Fatty Acids, Unsaturated; D008315:Malondialdehyde; D015237:Dinoprost; D007041:Hypoxanthine Phosphoribosyltransferase