title: A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae.
abstract: Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.
authors.affiliation: Department of Molecular Genetics, Max-Planck-Institute for Biophysical Chemistry, D-37070, Göttingen, Germany. aanna@gwdg.de
authors.forename: A
authors.initials: A
authors.lastname: De Antoni
authors.forename: D
authors.initials: D
authors.lastname: Gallwitz
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D000939:Epitopes / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D005819:Genetic Markers; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006639:Histidine / Q000235:genetics; D006801:Humans; D019426:Integrases; D007613:Kanamycin Resistance; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins
chemical_list: D004274:DNA, Recombinant; D000939:Epitopes; D005656:Fungal Proteins; C028391:G protein, vesicular stomatitis virus; D005819:Genetic Markers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D008562:Membrane Glycoproteins; D016271:Proto-Oncogene Proteins c-myc; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D006639:Histidine; C045073:Cre recombinase; D019426:Integrases