title: Age effect of type I collagen on morphogenesis of Mardin-Darby canine kidney cells.
abstract: BACKGROUND
  Mardin-Darby canine kidney (MDCK) cells cultured in hydrated collagen gels develop simple epithelial cysts or branching tubules, depending on the presence of hepatocyte growth factor (HGF). Constituents of extracellular matrix can modulate the morphogenesis of MDCK cells. Collagen is one of the few well-defined structural entities that display gross structural changes with aging. This study was conducted to delineate the effects of age-induced changes of collagen on the morphogenesis of MDCK cells cultured in collagen gel.
  
  
  METHODS
  We employed Y224 and MDCK clone II 3B5 cells to study cystogenesis and branching tubulogenesis, respectively. Cells were cultured in three-dimensional collagen gels prepared from 1-, 4-, 8-, and 16-month-old rat tail tendons, and their capacity to develop cysts or branching tubules was assessed. We also analyzed the compositions and physical structures of collagen of various ages.
  
  
  RESULTS
  Y224 cells developed generally larger spherical cysts in collagen gels prepared from rats that were more than four months old. The ratio of apoptosis of cells cultured in one-month-old collagen gel was markedly higher than in the gel of older ages. The results were consistent with the observations that collagen gel overlay-induced apoptosis of Y224 cells in one-month-old collagen was higher than that in older collagen. On the other hand, 3B5 cells exhibited a remarkable scattering morphology when cultured in one- or four-month-old collagen gel with HGF. In contrast, 3B5 cells exhibited more intercellular adhesion and were organized into branching tubule structures only in the collagen gel that was more than eight months old. The differences in morphogenesis could be explained by the observations that collagen of younger ages exerted markedly higher HGF-triggered migration capability than collagen of older ages.
  
  
  CONCLUSIONS
  Age-related alterations in collagen influence epithelial cell morphogenesis via regulation of cell apoptosis, proliferation, and/or motility.
authors.affiliation: Department of Physiology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors.forename: S T
authors.initials: ST
authors.lastname: Jiang
authors.forename: K K
authors.initials: KK
authors.lastname: Liao
authors.forename: M C
authors.initials: MC
authors.lastname: Liao
authors.forename: M J
authors.initials: MJ
authors.lastname: Tang
mesh_terms: D000143:Acids; D000375:Aging / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000502:physiology*; D003560:Cysts / Q000209:etiology; D004285:Dogs; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D007668:Kidney / Q000166:cytology*; D007674:Kidney Diseases / Q000209:etiology; D007684:Kidney Tubules / Q000187:drug effects / Q000254:growth & development; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
chemical_list: D000143:Acids; D017228:Hepatocyte Growth Factor; D003094:Collagen