title: Isolation and hypoglycemic activity of 5, 7,3'-trihydroxy-3,6,4'-trimethoxyflavone from Brickellia veronicaefolia.
abstract: Hypoglycemic activity-guided fractionation together with chemical analysis led to the isolation of one flavone (5, 7,3'-trihydroxy-3,6,4'-trimethoxyflavone) from the chloroform extract of the leaves of Brikkellia veronicaefolia. Identification was based on spectroscopic methods. The isolated flavone was tested for hypoglycemic activity in normal and alloxan-diabetic CD1 mice (25-30 g) were administered in doses of 10, 25 and 50 mg/kg body weight. The blood glucose levels were determined before and 1.5, 3, 4.5 and 24 hours after drug administration. The results showed that the flavone produces a significant hypoglycemic effect in normal as well as in diabetic mice. Comparison was made between the action of the flavone and a known hypoglycemic drug as tolbutamide (50 mg/kg). The flavone was found to be slow and less effective than tolbutamide.
authors.affiliation: Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingeniería Química e Industias Extractivas IPN, México D.F. México. dmex@dfl.telmex.net.mx
authors.forename: R M
authors.initials: RM
authors.lastname: Perez
authors.forename: H
authors.initials: H
authors.lastname: Cervantes
authors.forename: M A
authors.initials: MA
authors.lastname: Zavala
authors.forename: J
authors.initials: J
authors.lastname: Sanchez
authors.forename: S
authors.initials: S
authors.lastname: Perez
authors.forename: C
authors.initials: C
authors.lastname: Perez
mesh_terms: D000496:Alloxan; D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D001786:Blood Glucose / Q000187:drug effects*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D008815:Mice, Inbred Strains; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D001786:Blood Glucose; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C083161:centaureidin; D000496:Alloxan