title: Incorporation of a 4-hydroxy-N-acetylprolinol nucleotide analogue improves the 3'-exonuclease stability of 2'-5'-oligoadenylate-antisense conjugates.
abstract: Incorporation of a 4-hydroxy-N-acetylprolinol nucleotide analogue at the 3'-terminus of DNA or 2-5A-DNA sequences resulted in a significantly enhanced 3'-exonuclease resistance while the affinity for complementary RNA was only slightly decreased. Furthermore, the binding to and activation of human RNase L by thus modified 2-5A-DNA conjugates was not altered as compared to the parent unmodified 2-5A-DNAs.
authors.affiliation: Leiden Institute of Chemistry, Gorlaeus Laboratories, The Netherlands.
authors.forename: J C
authors.initials: JC
authors.lastname: Verheijen
authors.forename: A M
authors.initials: AM
authors.lastname: van Roon
authors.forename: N J
authors.initials: NJ
authors.lastname: Meeuwenoord
authors.forename: H R
authors.initials: HR
authors.lastname: Stuivenberg
authors.forename: S F
authors.initials: SF
authors.lastname: Bayly
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: G A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: P F
authors.initials: PF
authors.lastname: Torrence
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry*; D001483:Base Sequence; D016373:DNA, Antisense / Q000737:chemistry*; D004722:Endoribonucleases / Q000378:metabolism; D004789:Enzyme Activation; D005092:Exonucleases / Q000378:metabolism*; D006801:Humans; D009711:Nucleotides / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D000227:Adenine Nucleotides; D016373:DNA, Antisense; D009711:Nucleotides; D009843:Oligoribonucleotides; D012333:RNA, Messenger; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; D005092:Exonucleases; C022650:spleen exonuclease; C031956:2-5A-dependent ribonuclease