title: Histological and molecular mapping of adenocarcinoma of the oesophagus and gastroesophageal junction: loss of heterozygosity occurs in histologically normal epithelium in the oesophagus and stomach.
abstract: Seventy malignant, premalignant and histologically normal biopsies from 7 oesophagogastrectomy specimens of adenocarcinomas of the lower oesophagus and gastroesophageal junction were analysed for loss of heterozygosity (LOH) at 9 known or putative gene loci. LOH was detected in 20 of 27 (74%) malignant biopsies, 4 of 7 (57%) biopsies of dysplasia, 2 of 12 (25%) biopsies of histologically normal oesophagus adjacent to adenocarcinoma, and in 2 of 14 (14%) biopsies of histologically normal stomach adjacent to adenocarcinoma. LOH at the VHL, APC, CDKN2 and DCC tumour suppressor and MSH3 mismatch repair gene loci can be detected in histologically normal tissue and in adjacent adenocarcinoma, and are potential markers of early neoplastic progression.
authors.affiliation: Molecular Genetics and Oncology Group, Department of Clinical Dental Sciences, Liverpool L69 3BX, UK.
authors.forename: K
authors.initials: K
authors.lastname: Dolan
authors.forename: J
authors.initials: J
authors.lastname: Garde
authors.forename: S J
authors.initials: SJ
authors.lastname: Walker
authors.forename: R
authors.initials: R
authors.lastname: Sutton
authors.forename: J
authors.initials: J
authors.lastname: Gosney
authors.forename: J K
authors.initials: JK
authors.lastname: Field
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D020137:Base Pair Mismatch; D001706:Biopsy; D002874:Chromosome Mapping; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D016629:Esophagectomy; D004947:Esophagus / Q000473:pathology*; D005743:Gastrectomy; D005753:Gastric Mucosa / Q000473:pathology*; D005819:Genetic Markers; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000473:pathology; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology / Q000601:surgery
chemical_list: D005819:Genetic Markers