Steroidogenic alterations in testes and sera of rats exposed to trichloroethylene (TCE) by inhalation.
1. Trichloroethylene (TCE) is an organic unsaturated solvent used in dry cleaning, metal degreasing, thinner for paints/varnishes, anaesthetic agents etc. Human beings are considerably exposed to TCE vapours by inhalation route. 2. TCE has been reported to induce spontaneous abortions and congenital cardiac malformation in occupationally exposed women. However, scanty on-line information is available regarding toxic effects of TCE on male reproductive efficiency in experimental animals. 3. Our earlier observations with TCE inhalation in male rats (376 p.p.m., 4 h/day, 5 days a week) for 12 and 24 weeks using whole body dynamic inhalation chamber consistently showed significant decrease (P<0.05) in total epididymal sperm count and sperm motility. The mating experiments of above TCE inhaled rats with virgin unexposed females showed significantly decreased fertility. 4. These observations prompted us to investigate whether or not primary testicular steroidal precursors (cholesterol and ascorbic acid) and testosterone have any role in TCE induced significantly decreased epididymal sperm count, sperm motility and overall male reproductive inefficiency resulting therefrom. 5. The results indicate significant decrease (P<0.05) in total epididymal sperm count, sperm motility, specific activities of enzymes Glucose 6-p dehydrogenase (G6PDH) and 17 beta hydroxy steroid dehydrogenase (17betaHSD) with concomitant decrease in serum testosterone concentrations in TCE inhaled rats showing reduced male reproductive efficiency. There was net accumulation in total cholesterol contents in testes of TCE exposed rats. 6. The findings in the present study indicate possible impairment of testosterone biosynthesis in TCE inhaled rats after 12 and 24 weeks. These findings also serve in parts to elucidate the mechanism of reproductive inefficiency in TCE exposed rats. The role of testosterone in this phenomenon is being reported for the first time.
Kumar
P
P
Inhalation Toxicology Laboratory and Animal House, Industrial Toxicology Research Centre, Lucknow, UP, India.
Prasad
A K
AK
Dutta
K K
KK
D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000280:Administration, Inhalation; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000008:administration & dosage / Q000633:toxicity*; D013076:Sperm Count / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood*; D014241:Trichloroethylene / Q000008:administration & dosage / Q000633:toxicity*
D012997:Solvents; D014241:Trichloroethylene; D013739:Testosterone; D002784:Cholesterol; D015067:17-Hydroxysteroid Dehydrogenases; D005954:Glucosephosphate Dehydrogenase; D001205:Ascorbic Acid