title: Chizé virus, a new phlebovirus isolated in France from Ixodes (Trichotoixodes) frontalis.
abstract: A new phlebovirus (Bunyaviridae family, Phlebovirus genus), provisionally designed Chizé virus, was isolated from a nymph of Ixodes (Trichotoixodes) frontalis collected on a wren (Troglodytes troglodytes) found dead in the Chizé forest, western France. Chizé virus produced a lethal encephalitis in one-day-old mice and cytopathic effect (CPE) in Vero cells. Extracellular particles with a mean diameter of 105 nm with surface spikes characteristic of Uukuniemi (UUK) serogroup viruses were observed in Vero cells. Chizé virus reacted in complement-fixation test with several UUK serogroup viruses but was readily distinguished from all registered viruses in the serogroup. I. frontalis is highly specific for birds and unlikely to transmit Chizé virus to humans or domestic animals; the pathogenicity of the new virus to wild birds remains to be clarified.
authors.affiliation: Laboratoire de Virologie, Faculté de Médecine, Brest, France. chastelc@aol.com
authors.forename: C
authors.initials: C
authors.lastname: Chastel
authors.forename: L
authors.initials: L
authors.lastname: Chandler
authors.forename: F
authors.initials: F
authors.lastname: Le Goff
authors.forename: O
authors.initials: O
authors.lastname: Chastel
authors.forename: R
authors.initials: R
authors.lastname: Tesh
authors.forename: R
authors.initials: R
authors.lastname: Shope
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002044:Bunyaviridae Infections / Q000473:pathology / Q000821:virology*; D002522:Chlorocebus aethiops; D003168:Complement Fixation Tests; D003588:Cytopathogenic Effect, Viral; D018792:Encephalitis, Viral / Q000473:pathology / Q000821:virology*; D005602:France; D018884:Ixodes / Q000821:virology*; D051379:Mice; D016856:Phlebovirus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity*; D012703:Serotyping; D014709:Vero Cells
chemical_list: D000956:Antigens, Viral