Design of artificial pepetides that recognize the HIV RRE IIB RNA.
Peptide nucleic acid (PNA) conjugated peptides, derived from HIV-1 Rev, were designed and synthesized in order to construct molecules that recognize HIV RRE IIB RNA. The competitive binding analyses using fluorescent Rev peptide revealed that the PNA unit on the peptide affected the RNA binding.
Takahashi
T
T
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Kumagai
I
I
Hamasaki
K
K
Ueno
A
A
Mihara
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D015195:Drug Design; D015694:Gene Products, rev / Q000737:chemistry*; D015497:HIV-1; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry*; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D007202:Indicators and Reagents; D020135:Peptide Nucleic Acids; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus