title: A novel member of the testis specific serine kinase family, tssk-3, expressed in the Leydig cells of sexually mature mice.
abstract: We have recently characterized two members of a novel family of murine testis specific serine kinases, tssk-1 and tssk-2, expressed exclusively in spermatids undergoing spermiogenesis. Using a differential screening approach we have isolated a third family member, tssk-3. The open reading frame of tssk-3 encodes a protein of 275 amino acids, consisting essentially of a serine/threonine protein kinase domain only. In contrast, tssk-1 and -2 have distinct, approximately 100 amino acid domains located C-terminally to the kinase domain. Immunoprecipitation experiments revealed that while tssk-1 and tssk-2 form detergent resistant complexes, tssk-3 is not associated with either protein. Expression of tssk-3 was induced at puberty, persisted during adulthood and was restricted to the interstitial Leydig cells of post-pubertal males.
authors.affiliation: The Tiefenau Laboratory, Department of Clinical Research, University of Berne, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Zuercher
authors.forename: V
authors.initials: V
authors.lastname: Rohrbach
authors.forename: A C
authors.initials: AC
authors.lastname: Andres
authors.forename: A
authors.initials: A
authors.lastname: Ziemiecki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D015870:Gene Expression; D007985:Leydig Cells / Q000201:enzymology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology
chemical_list: D018076:DNA, Complementary; C486554:Tesk2 protein, mouse; C097507:testis-specific protein kinase 1; D017346:Protein Serine-Threonine Kinases; C412363:tssk3 protein, mouse