Mitochondrial ATPase 6 gene expression in unfertilized oocytes and cleavage-stage embryos.
OBJECTIVE
To compare the level of mitochondrial ATPase 6 gene expression in unfertilized oocytes and cleavage-stage embryos.


METHODS
Reverse transcription polymerase chain reaction was performed in unfertilized oocytes and cleavage-stage embryos derived from tripronucleate embryos to determine ATPase 6 gene expression.


METHODS
Department of Obstetrics and Gynecology, Human Genetics Laboratory, Infertility Medical Center of CHA General Hospital, College of Medicine, Pochon CHA University, Seoul, Korea.


METHODS
Oocytes were obtained from infertile couples undergoing in vitro fertilization.


METHODS
Unfertilized oocytes collected at 48 hours after retrieval and cleavage-stage embryos derived from tripronucleate embryos were prepared for evaluation of mitochondrial gene expression.


METHODS
Comparison of ATPase 6 gene expression by using single-cell reverse transcription polymerase chain reaction.


RESULTS
Expression of unfertilized oocytes decreased compared with early cleavage-stage embryos.


CONCLUSIONS
Our findings of decreased ATPase 6 expression in unfertilized oocytes suggest that there may be a decrease in the mitochondrial functional capacity of oxidative phosphorylation.
Lee
S H
SH
CHA General Hospital, and Pochon CHA University, Seoul, South Korea.
Han
J H
JH
Cho
S W
SW
Cha
K E
KE
Park
S E
SE
Cha
K Y
KY
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D003720:Densitometry; D004622:Embryo, Mammalian / Q000201:enzymology*; D005260:Female; D005307:Fertilization in Vitro; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D025261:Mitochondrial Proton-Translocating ATPases; D009865:Oocytes / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy
C501005:MT-ATP6 protein, human; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases