title: Strain diversity in major surface protein 2 expression during tick transmission of Anaplasma marginale.
abstract: Specific major surface protein 2 (MSP2) variants are expressed by Anaplasma marginale within the tick salivary gland and, following transmission, are expressed during acute rickettsemia. In previous work, we have shown that a restricted pattern of MSP2 variants is expressed in the salivary glands of Dermacentor andersoni ticks infected with the South Idaho strain of A. marginale. Now we demonstrate that the identical restriction does not apply to two other strains of A. marginale, and that different variants are also expressed when the same strain is transmitted by different Dermacentor spp. This indicates that antigenic diversity among strains is maintained in tick transmission and may be a significant constraint to MSP2 vaccine development.
authors.affiliation: Program in Vector-Borne Diseases, Washington State University, Pullman, Washington 99164-7040, USA.
authors.forename: F R
authors.initials: FR
authors.lastname: Rurangirwa
authors.forename: D
authors.initials: D
authors.lastname: Stiller
authors.forename: G H
authors.initials: GH
authors.lastname: Palmer
mesh_terms: D000595:Amino Acid Sequence; D000709:Anaplasma / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000940:Antigenic Variation / Q000235:genetics*; D000942:Antigens, Bacterial / Q000145:classification / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003870:Dermacentor / Q000382:microbiology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C103348:msp2 protein, Anaplasma marginale