A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae.
Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.
De Antoni
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Department of Molecular Genetics, Max-Planck-Institute for Biophysical Chemistry, D-37070, Göttingen, Germany. aanna@gwdg.de
Gallwitz
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D000939:Epitopes / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D005819:Genetic Markers; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D006639:Histidine / Q000235:genetics; D006801:Humans; D019426:Integrases; D007613:Kanamycin Resistance; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins
D004274:DNA, Recombinant; D000939:Epitopes; D005656:Fungal Proteins; C028391:G protein, vesicular stomatitis virus; D005819:Genetic Markers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D008562:Membrane Glycoproteins; D016271:Proto-Oncogene Proteins c-myc; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D006639:Histidine; C045073:Cre recombinase; D019426:Integrases