Gonadotropin-releasing hormones in the brain and pituitary of the teleost, the white sucker.
The present study investigated GnRH forms within the brain of a representative of the order Cypriniformes, the white sucker, Catostomus commersoni, using HPLC, RIA, and immunocytochemistry. Several immunoreactive (ir) GnRH forms were identified in the brain of the white sucker by chromatography and radioimmunoassay, including ir-salmon GnRH, ir-lamprey GnRH-I and -III, and ir-chicken GnRH-II. Results from immunocytochemical studies were consistent with multiple GnRH forms distributed in different patterns, particularly for fibers. Neuronal perikarya containing ir-salmon GnRH and ir-lamprey-like GnRH were found laterally within the preoptic area and rostral hypothalamus. Cells containing exclusively ir-salmon GnRH appeared slightly more rostrally, but in the same region. Fibers containing ir-salmon GnRH and ir-lamprey-like GnRH were seen throughout the caudal telencephalon and extended into the diencephalon, toward the pituitary. Fibers containing ir-chicken-II-like GnRH were also seen in the caudal telencephalon, but were concentrated more dorsally in the diencephalon. Within the pituitary, fibers containing ir-salmon GnRH and ir-lamprey-like GnRH entered the neurohypophysis, but differed in their destinations. Fibers containing ir-salmon GnRH remained within the neurohypophysis, while fibers containing ir-lamprey-like GnRH targeted adenohypophyseal tissue. These findings are consistent with the hypothesis that multiple GnRH forms with multiple functions exist within the brain and pituitary of teleosts and provide further evidence of a lamprey-like GnRH within an early evolved teleost species.
Robinson
T C
TC
Department of Biochemistry and Molecular Biology, Biological Science Center, University of New Hampshire, Durham 03824, USA.
Tobet
S A
SA
Chase
C
C
Waldron
T
T
Sower
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003531:Cypriniformes / Q000378:metabolism*; D004027:Diencephalon / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis*; D007031:Hypothalamus / Q000737:chemistry; D007150:Immunohistochemistry; D007798:Lampreys; D011301:Preoptic Area / Q000737:chemistry; D011863:Radioimmunoassay; D012474:Salmon; D017385:Sequence Homology; D013687:Telencephalon / Q000737:chemistry
D007987:Gonadotropin-Releasing Hormone