title: Role of lipids in the translocation of proteins across membranes.
abstract: The architecture of cells, with various membrane-bound compartments and with the protein synthesizing machinery confined to one location, dictates that many proteins have to be transported through one or more membranes during their biogenesis. A lot of progress has been made on the identification of protein translocation machineries and their sorting signals in various organelles and organisms. Biochemical characterization has revealed the functions of several individual protein components. Interestingly, lipid components were also found to be essential for the correct functioning of these translocases. This led to the idea that there is a very intimate relationship between the lipid and protein components that enables them to fulfil their intriguing task of transporting large biopolymers through a lipid bilayer without leaking their contents. In this review we focus on the Sec translocases in the endoplasmic reticulum and the bacterial inner membrane. We also highlight the interactions of lipids and proteins during the process of translocation and integrate this into a model that enables us to understand the role of membrane lipid composition in translocase function.
authors.affiliation: Department of Biochemistry of Membranes, CBLE, Institute Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands. F.vanvoorst@chem.uu.nl
authors.forename: F
authors.initials: F
authors.lastname: Van Voorst
authors.forename: B
authors.initials: B
authors.lastname: De Kruijff
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins; D005057:Eukaryotic Cells / Q000201:enzymology / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D010743:Phospholipids / Q000378:metabolism; D021382:Protein Sorting Signals / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D015003:Yeasts / Q000201:enzymology / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008563:Membrane Lipids; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010743:Phospholipids; D021382:Protein Sorting Signals; D011506:Proteins; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins