A sequence-ready physical map of the region containing the human natural killer gene complex on chromosome 12p12.3-p13.2.
We developed a sequence-ready physical map of a part of human chromosome 12p12.3-p13.2 where the natural killer gene complex (NKC) is located. The NKC includes a cluster of genes with structure similar to that of the Ca(2+)-dependent lectin superfamily of glycoproteins that are expressed on the surface of most natural killer (NK) cells and a subset of T cells. These killer cell lectin-like receptors (KLR) are involved in NK target cell recognition, leading to activation or inhibition of NK cell function. We used a number of sequence-tagged site (STS) markers from this region to screen two large insert bacterial artificial chromosome (BAC) libraries and a bacteriophage P1-derived (PAC) chromosome library. The clones were assembled into contiguous sets by STS content analysis. The 72-BAC and 11-PAC contig covers nearly 2 Mb of DNA and provides an average marker resolution of 26 kb. We have precisely localized 17 genes, 5 expressed sequence tags, and 49 STSs within this contig. Of this total number of STS, 30 are newly developed by clone-end sequencing. We established the order of the genes as tel-M6PR-MAFAL (HGMW-approved symbol KLRG1)-A2M-PZP-A2MP-NKRP1A (HGMW-approved symbol KLRB1)-CD69-AICL (HGMW-approved symbol CLECSF2)-KLRF1-OLR1-CD94 (HGMW-approved symbol KLRD1)-NKG2D (HGMW-approved symbol D12S2489E)-PGFL-NKG2F (HGMW-approved symbol KLRC4)-NKG2E (HGMW-approved symbol KLRC3)-NKG2A (HGMW-approved symbol KLRC1)-LY49L (HGMW-approved symbol KLRA1)-cen. This map would facilitate the cloning of new KLR genes and the complete sequencing of this region.
Renedo
M
M
Unidad de Biología Molecular, Hospital Universitario de la Princesa, Madrid, 28006, Spain.
Arce
I
I
Montgomery
K
K
Roda-Navarro
P
P
Lee
E
E
Kucherlapati
R
R
Fernández-Ruiz
E
E
D015703:Antigens, CD / Q000235:genetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D017931:DNA Primers; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D037102:Lectins / Q000235:genetics; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D051920:NK Cell Lectin-Like Receptor Subfamily D; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics; D016324:Sequence Tagged Sites; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017931:DNA Primers; D037102:Lectins; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D051920:NK Cell Lectin-Like Receptor Subfamily D; D011956:Receptors, Cell Surface; D000099192:CD69 Antigens; C119621:Clec4n protein, mouse; C497869:KLRD1 protein, human