Illegitimate villin transcripts in normal bone marrow precludes detection of colon cancer micrometastases.
Villin is a specific marker for normal and tumoral colon tissue. We have developed a highly sensitive assay using reverse transcription (RT) and real-time PCR to detect villin transcripts. The sensitivity of detection is one colon cancer cell. However, high levels of illegitimate villin transcripts were observed in normal bone marrow, precluding the use of villin RT-PCR for routine detection of colon cancer cells in bone marrow of patients with colon cancer.
Salbe
C
C
Center for the Study of Biological Markers of Malignancy, Venice Regional Hospital, Italy.
de Cremoux
P
P
Bonneton
C
C
Manet
S
S
Almeida
A
A
Magdelénat
H
H
Bourstyn
E
E
Robine
S
S
D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics*; D001853:Bone Marrow / Q000473:pathology*; D001854:Bone Marrow Cells / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D008840:Microfilament Proteins / Q000032:analysis / Q000235:genetics*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D015415:Biomarkers; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D008840:Microfilament Proteins; C026615:villin