Adaptation of reptilian paramyxovirus to mammalian cells (Vero cells).
The reptilian paramyxovirus GOV was successfully adapted to Vero cells in 80 passages at 30 degrees C. The virus replicated with HA titres of 1:64 and 10(7.5) TCID50/ml in the heterologous host cells forming syncytia, giant cells and cytoplasmic inclusion bodies. After 80 passages GOV was identified by immunofluorescence, by immune electron microscopy and by PCR. Sequencing of RT-PCR products of GOV after 80 passages did not reveal changes in the nucleotide sequence of the partial L-gene of GOV.
Mayr
A
A
Institute for Medical Microbiology, Infectious and Epidemic Diseases, University of Munich, Germany.
Franke
J
J
Ahne
W
W
D000818:Animals; D002522:Chlorocebus aethiops; D017812:Colubridae / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006386:Hemagglutination Tests / Q000662:veterinary; D016253:Microscopy, Immunoelectron / Q000662:veterinary; D018184:Paramyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D018108:Respirovirus / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D014709:Vero Cells
D004279:DNA, Viral