A possible function of DNA curvature in a vector system that allows efficient expression of heterologous proteins in CHO cells.
A cis-acting element derived from the nontranscribed spacer region of murine rDNA allows efficient expression of heterologous proteins in transformed Chinese hamster ovary (CHO) cells. Analysis of the structural conformation of this cis-acting element revealed that it has a DNA curvature. Whether the DNA curvature plays any roles in regulating expression of heterologous proteins or not has been studied.
Hirota
Y
Y
Meiji Cell Technology Center, Odawara, Japan.
Maeda
T
T
Yan
H
H
Nakamichi
N
N
Matsumura
T
T
D000818:Animals; D001482:Base Composition; D016466:CHO Cells; D006224:Cricetinae; D004275:DNA, Ribosomal / Q000737:chemistry* / Q000235:genetics*; D005822:Genetic Vectors; D008156:Luciferases / Q000032:analysis / Q000235:genetics; D051379:Mice; D009690:Nucleic Acid Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection
D004275:DNA, Ribosomal; D011994:Recombinant Proteins; D008156:Luciferases