title: Cytoadherence characteristics of Plasmodium falciparum isolates in Thailand using an in vitro human lung endothelial cells model.
abstract: Using an in vitro model of human lung endothelial cells, we studied different characteristics of Plasmodium falciparum isolates as potential factors for malaria severity in 2 Thai patient groups: 27 with complicated malaria and 42 with uncomplicated malaria. In regard to binding properties, no association existed between cytoadherence and rosette phenotypes (P = 0.1) and hypothrombocytemia increased the cytoadherence level (P = 0.007). Cytoadherence was significantly associated with malaria severity (P = 0.05) in contrast to rosette formation (P = 0.9). Intercellular adhesion molecule-1 and chondroitin-4-sulfate were major receptors of cytoadherence in those with complicated malaria compared with those with uncomplicated malaria (P < 10(-4)). Chondroitin-4-sulfate could act as a putative receptor for malaria complications in non-pregnant women. CD36 was the main receptor in patients with uncomplicated malaria (P < 10(-3)). Vascular cell adhesion molecule-1 and E-selectin played a minor role in 2 groups (P = 0.6). Qinghaosu derivatives were more efficient than other antimalarial drugs, but a positive correlation was observed between the 50% inhibitory concentrations of halofantrine and quinine and the number of adhesive parasitized red blood cells, suggesting their influence on cytoadherence.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale Unite 511, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Traoré
authors.forename: K
authors.initials: K
authors.lastname: Muanza
authors.forename: S
authors.initials: S
authors.lastname: Looareesuwan
authors.forename: S
authors.initials: S
authors.lastname: Supavej
authors.forename: S
authors.initials: S
authors.lastname: Khusmith
authors.forename: M
authors.initials: M
authors.lastname: Danis
authors.forename: P
authors.initials: P
authors.lastname: Viriyavejakul
authors.forename: F
authors.initials: F
authors.lastname: Gay
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000962:Antimalarials / Q000494:pharmacology; D001667:Binding, Competitive / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000502:physiology; D004727:Endothelium / Q000473:pathology; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D008168:Lung / Q000473:pathology*; D016778:Malaria, Falciparum / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018512:Parasitemia; D010616:Phenanthrenes / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000737:chemistry*; D011803:Quinine / Q000008:administration & dosage / Q000627:therapeutic use; D012397:Rosette Formation; D013785:Thailand
chemical_list: D000911:Antibodies, Monoclonal; D000962:Antimalarials; D010616:Phenanthrenes; D018799:Intercellular Adhesion Molecule-1; D002809:Chondroitin Sulfates; D011803:Quinine; C023768:halofantrine