Development of a short Ca2+-dependent deoxyribozyme with RNA cleavage activity.
We developed a short Ca2+-dependent deoxyribozyme with 11 mer catalytic loop domain (dGGCTACAACGA) that catalyzed site-specific RNA cleavage reaction between rA and rU. The second-order rate constant of this short deoxyribozyme is 1.7 x 10(7) M(-1) min(-1) at 37 degrees C, and this value is very similar to that of the deoxyribozyme (dGGCTAGCTACAACGA) in the presence of Ca2+.
Sugimoto
N
N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Okumoto
Y
Y
D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D002384:Catalysis; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012313:RNA / Q000378:metabolism*; D013379:Substrate Specificity
D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D012313:RNA; D002118:Calcium