title: Mitochondrial ATPase 6 gene expression in unfertilized oocytes and cleavage-stage embryos.
abstract: OBJECTIVE
  To compare the level of mitochondrial ATPase 6 gene expression in unfertilized oocytes and cleavage-stage embryos.
  
  
  METHODS
  Reverse transcription polymerase chain reaction was performed in unfertilized oocytes and cleavage-stage embryos derived from tripronucleate embryos to determine ATPase 6 gene expression.
  
  
  METHODS
  Department of Obstetrics and Gynecology, Human Genetics Laboratory, Infertility Medical Center of CHA General Hospital, College of Medicine, Pochon CHA University, Seoul, Korea.
  
  
  METHODS
  Oocytes were obtained from infertile couples undergoing in vitro fertilization.
  
  
  METHODS
  Unfertilized oocytes collected at 48 hours after retrieval and cleavage-stage embryos derived from tripronucleate embryos were prepared for evaluation of mitochondrial gene expression.
  
  
  METHODS
  Comparison of ATPase 6 gene expression by using single-cell reverse transcription polymerase chain reaction.
  
  
  RESULTS
  Expression of unfertilized oocytes decreased compared with early cleavage-stage embryos.
  
  
  CONCLUSIONS
  Our findings of decreased ATPase 6 expression in unfertilized oocytes suggest that there may be a decrease in the mitochondrial functional capacity of oxidative phosphorylation.
authors.affiliation: CHA General Hospital, and Pochon CHA University, Seoul, South Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Han
authors.forename: S W
authors.initials: SW
authors.lastname: Cho
authors.forename: K E
authors.initials: KE
authors.lastname: Cha
authors.forename: S E
authors.initials: SE
authors.lastname: Park
authors.forename: K Y
authors.initials: KY
authors.lastname: Cha
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D003720:Densitometry; D004622:Embryo, Mammalian / Q000201:enzymology*; D005260:Female; D005307:Fertilization in Vitro; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D025261:Mitochondrial Proton-Translocating ATPases; D009865:Oocytes / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy
chemical_list: C501005:MT-ATP6 protein, human; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases