Efficacy of an inactivated vaccine for equine herpesvirus type 1 in a novel hamster model.
A vaccine designated F.EHV1(S(-))BHK, which was prepared by formaldehyde treatment of equine herpesvirus type 1 (EHV1)-infected baby hamster kidney cells, stimulated neutralising antibodies in hamsters and rabbits and protected new-born hamsters against a lethal challenge with EHV1 by vaccination of their pregnant mothers. The preparative method was then modified to eliminate virus particles and intraparticulate DNA, producing a vaccine designated Bg.F.EHV1(S(-))BHK. this modification stimulated neutralising antibodies with evidence of in vivo inactivation of the virus above non-specific levels in a mouse model. There were no adverse effects from these vaccines in rodent or rabbit species.
Skinner
G R
GR
Department of Infection, University of Birmingham, Birmingham, UK.
Davies
J
J
D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D004195:Disease Models, Animal; D005260:Female; D005374:Filtration; D005557:Formaldehyde; D006566:Herpesviridae Infections / Q000517:prevention & control*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D007112:Immunity, Maternally-Acquired; D051379:Mice; D008809:Mice, Inbred C3H; D009500:Neutralization Tests; D011247:Pregnancy; D011817:Rabbits; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000009:adverse effects / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000009:adverse effects / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015164:Vaccines, Inactivated; D014764:Viral Proteins; D014765:Viral Vaccines; D005557:Formaldehyde