Structural and functional properties of Langmuir films of antibodies based on amphiphilic polyelectrolytes.
We optimized the procedure for the formation of Langmuir films of antibodies based on amphiphilic polyelectrolytes and studied the physicochemical and immunochemical properties of the films obtained. Their immunochemical properties were compared with the immunochemical activity of antibodies in Langmuir films without amphiphilic polyelectrolytes and with antibodies adsorbed on the surface of polystyrene and graphite. The efficiency of immune adsorption by the films based on amphiphilic polyelectrolytes was shown to be greater; the affinity of antibodies and surface concentration of their active conformation depended on the type of amphiphilic polyelectrolytes used to obtain the films. We investigated the structure of these films at the surface of highly oriented pyrolytic graphite using the method of atomic force microscopy. Changes in the structure of the films under study caused by the increase of surface pressure were demonstrated.
Budashov
I A
IA
Research Center of Molecular Diagnostics and Therapy, Moscow, Russia.
Kurochkin
I N
IN
Tsibezov
V V
VV
Kalnov
S L
SL
Denisov
A K
AK
Kiselyova
O I
OI
Yaminsky
I V
IV
D000906:Antibodies / Q000276:immunology* / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods; D004573:Electrolytes / Q000378:metabolism*; D007163:Immunosorbent Techniques; D008567:Membranes, Artificial; D011108:Polymers / Q000378:metabolism*
D000906:Antibodies; D004573:Electrolytes; D008567:Membranes, Artificial; D011108:Polymers