title: A sensitive docetaxel assay in plasma by solid-phase extraction and high performance liquid chromatography-UV detection: validation and suitability in phase I clinical trial pharmacokinetics.
abstract: We have developed a specific and sensitive method aiming at docetaxel (Taxotere) determination in plasma of treated patients. This involved solid-phase extraction of 1 ml of plasma onto carboxylic acid (CBA) grafted silica cartridges followed by reversed-phase liquid chromatography with UV detection. The best selectivity was obtained through the use of C18 Uptisphere as stationary phase. The low limit of quantitation obtained (LOQ: 5 ng/ml) allowed measurements of docetaxel up to 24 hours after one-hour infusions with low dosages of drug (60 mg/m2). The method was applied successfully to monitor docetaxel plasma levels within two protocols associating fixed dosages of either methotrexate or gemcitabine with escalating doses of Taxotere.
authors.affiliation: Laboratory of Pharmacokinetics, Centre Léon Bérard, Lyon, France.
authors.forename: C J
authors.initials: CJ
authors.lastname: Ardiet
authors.forename: B
authors.initials: B
authors.lastname: Tranchand
authors.forename: S
authors.initials: S
authors.lastname: Zanetta
authors.forename: A
authors.initials: A
authors.lastname: Guillot
authors.forename: E
authors.initials: E
authors.lastname: Bernard
authors.forename: M
authors.initials: M
authors.lastname: Peguy
authors.forename: P
authors.initials: P
authors.lastname: Rebattu
authors.forename: J P
authors.initials: JP
authors.lastname: Droz
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000097:blood; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D000971:Antineoplastic Combined Chemotherapy Protocols; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003841:Deoxycytidine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood; D000077143:Docetaxel; D006801:Humans; D008727:Methotrexate / Q000008:administration & dosage / Q000097:blood; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000097:blood; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D043823:Taxoids; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D012997:Solvents; D043823:Taxoids; D003841:Deoxycytidine; D000077143:Docetaxel; D017239:Paclitaxel; D008727:Methotrexate; D000093542:Gemcitabine