title: Cell-cell and cell-substrate adhesion in cultured Drosophila imaginal disc cells.
abstract: Drosophila imaginal disc cell lines were used to investigate various aspects of cellular adhesion in vitro. The distribution of PS integrins and their involvement in cell-cell and cell-substrate adhesion were assessed with the monoclonal antibody aBG-1 against the betaPS subunit, and both forms of adhesion were found to be impeded by the presence of the antibody. Adhesion to a number of extracellular matrix components was investigated, and the cells were found to adhere to human fibronectin. This adhesion was inhibited by aBG-1. The adhesion molecule fasciclin III was also found in these cells. Given that the cells are competent to perform cell-cell and cell-substrate adhesion, it was thought that apical basal polarity might be restored when other suitable conditions were provided, i.e., an artificial basement layer with feeder cells to provide nutrients basally to the cells, and some features of apical-basal morphology were seen in cells cultured under these conditions.
authors.affiliation: School of Biology, University of St. Andrews, St. Andrews Fife, United Kingdom.
authors.forename: A S
authors.initials: AS
authors.lastname: Miller
authors.forename: D M
authors.initials: DM
authors.lastname: Cottam
authors.forename: M J
authors.initials: MJ
authors.lastname: Milner
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis; D002449:Cell Aggregation; D002460:Cell Line; D004330:Drosophila / Q000166:cytology*; D029721:Drosophila Proteins; D006801:Humans; D039001:Integrin alpha Chains; D016023:Integrins / Q000096:biosynthesis
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; C061565:Fas3 protein, Drosophila; D039001:Integrin alpha Chains; D016023:Integrins; C082188:if protein, Drosophila