Enzymes and membrane proteins of ADSOL-preserved red blood cells.
BACKGROUND
The preservative solution ADSOL (adenine, dextrose, sorbitol, sodium chloride and mannitol) maintains red cell viability for blood trans-fusion for 6 weeks. It would be useful to know about its preservation qualities over longer periods.


OBJECTIVE
To determine some red cell biochemical parameters for peri-ods of up to 14 weeks in order to determine whether the red cell metabo-lism integrity would justify further studies aiming at increasing red cell preservation and viability.


METHODS
Biochemical evaluation designed to study red cell preservation.


METHODS
São Paulo University erythrocyte metabolism referral center.


METHODS
Six normal blood donors from the University Hospital of the Universidade Federal do Paraná, Curitiba, Brazil.


METHODS
Weekly assay of erythrocyte adenosine-5;-triphosphate (ATP), 2, 3-diphosphoglycerate (2,3DPG), hexokinase (HX), phosphofructokinase (PFK), pyruvate kinase (PK), glucose-6-phosphate dehydrogenase (G-6-PD), 6-phosphogluconic dehydrogenase (6-PGD), glyceraldehyde-3-phosphate dehydrogenase (GAPD), glutathione reduc-tase (GR), glutathione peroxidase (GSHPx), plasma sodium and potas-sium, blood pH, and membrane proteins of red cells preserved in ADSOL were studied during storage for 14 weeks storage.


RESULTS
During ADSOL preservation, erythrocyte ATP concentration decreased 60% after 5 weeks, and 90% after 10 weeks; the pH fell from 6.8 to 6. 4 by the 14th week. 2,3-DPG concentration was stable during the first week, but fell 90% after 3 weeks and was exhausted after 5 weeks. By the end of the 5th week, an activity decrease of 16-30% for Hx, GAPD, GR, G-6-PD and 6-PGD, 35% for PFK and GSHPx, and 45% for PK were observed. Thereafter, a uniform 10% decay was observed for all enzymes up to the 14th week. The red blood cell membrane pro-teins did not show significant alterations in polyacrylamide gel electro-phoresis (SDS-PAGE) during the 14 weeks.


CONCLUSIONS
Although the blood viability was shown to be poor from the 6th week up to the 14th week of storage due to ATP and 2,3-DPG depletion, the other biochemical parameters remained in fairly good condition for longer storage. As there is a gradual and uniform decay in activity throughout these 14 weeks, it seems that ADSOL-preserved red cells may be used as red cell enzyme standards and membrane proteins as well.
Leonart
M S
MS
Divisão de Saúde, Universidade Federal do Paraná, Curitiba, Brazil.
Nascimento
A J
AJ
Nonoyama
K
K
Pelissari
C B
CB
Barretto
O C
OC
D019794:2,3-Diphosphoglycerate / Q000032:analysis; D000225:Adenine; D000255:Adenosine Triphosphate / Q000032:analysis; D000328:Adult; D001793:Blood Preservation; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000201:enzymology*; D005260:Female; D005947:Glucose; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008353:Mannitol; D008565:Membrane Proteins / Q000032:analysis*; D008875:Middle Aged; D010088:Oxidoreductases / Q000032:analysis; D012965:Sodium Chloride
C037839:ADSOL; D008565:Membrane Proteins; D019794:2,3-Diphosphoglycerate; D008353:Mannitol; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D010088:Oxidoreductases; D005947:Glucose; D000225:Adenine