title: Development of an HIV-1-dependent expression vector with the Cre/loxP system.
abstract: Previously, we used the human methionine tRNA promoter as an expression cassette for hammerhead ribozymes. The tRNA promoter driven ribozyme was targeted against the LTR portion of the HIV-1 NL4-3 strain. We constructed VSV-G-pseudotyped MuLV-based vectors expressing the ribozyme. The ribozyme expressing retrovirus vector strongly suppressed gag p24 antigen production in freshly HIV-1 infected MT-4 cells. In this study, the potential of such a molecular genetic intervention was examined by using the Cre-loxP recombination system. Site-specific excision of HIV-1 was achieved by using this model system with an acute infection. These studies represent one step toward the development of a novel antiviral strategy for the treatment of AIDS.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Habu
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: N
authors.initials: N
authors.lastname: Miyano-Kurosaki
authors.forename: K
authors.initials: K
authors.lastname: Takai
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D005822:Genetic Vectors; D016655:HIV Core Protein p24 / Q000235:genetics; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D019426:Integrases / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012358:RNA, Transfer, Met / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014764:Viral Proteins
chemical_list: D016655:HIV Core Protein p24; D012358:RNA, Transfer, Met; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D008156:Luciferases; C045073:Cre recombinase; D019426:Integrases