title: Immunosuppressive activity in buffalo placenta.
abstract: Immunosuppressive activity in buffalo placenta was evaluated by measuring proliferation of lymphocytes in presence of phytohaemagglutinin (PHA) alone or PHA plus placental proteins. The immunosuppressive activity was dose-dependent over the protein concentration range of 10-50 micrograms/ml. Proteins from both cotyledon and non-cotyledon portions of placenta exhibited immunosuppressive activity. Fractions obtained with 0-40, 40-60 and 60-80% saturated ammonium sulphate exhibited 70, 73 and 75% suppression, respectively. PBS-soluble placental proteins were resolved on G-100 column into three peaks that exhibited 69 (peak 1), 55 (peak 2) and 73% (peak 3) suppression. Exogenously added interleukin-1 (IL-1) failed to reverse the suppression caused by buffalo placental proteins.
authors.affiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal, India.
authors.forename: S
authors.initials: S
authors.lastname: Mehta
authors.forename: Y S
authors.initials: YS
authors.lastname: Rajput
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000276:immunology*; D005260:Female; D007108:Immune Tolerance; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008213:Lymphocyte Activation; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D007375:Interleukin-1; D011257:Pregnancy Proteins