Action of chlorogenic acid in vegetables and fruits as an inhibitor of 8-hydroxydeoxyguanosine formation in vitro and in a rat carcinogenesis model.
Various plant extracts, such as carrot, burdock (gobou), apricot and prune, showed inhibitory effects in an in vitro assay of lipid peroxide-induced 8-hydroxydeoxyguanosine (8-OH-dG) formation. The major inhibitor purified from various plants extracts was identified as chlorogenic acid (CA), on the basis of UV- and mass-spectra and comparison with a standard sample. To examine whether CA also inhibits 8-OH-dG formation in animal organs, an oxygen radical-forming carcinogen, 4-nitroquinoline-1-oxide, was administered to rats, with or without CA. The 8-OH-dG level in the DNA of the rat tongue, the target organ, was significantly reduced in the CA-treated group.
Kasai
H
H
Department of Environmental Oncology, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan.
Fukada
S
S
Yamaizumi
Z
Z
Sugie
S
S
Mori
H
H
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D016588:Anticarcinogenic Agents / Q000032:analysis / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity; D002726:Chlorogenic Acid / Q000032:analysis / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000302:isolation & purification; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D005609:Free Radicals / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D011916:Rats, Inbred F344; D013056:Spectrophotometry, Ultraviolet; D014059:Tongue / Q000473:pathology; D014675:Vegetables / Q000737:chemistry*
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D005609:Free Radicals; D002726:Chlorogenic Acid; D015112:4-Nitroquinoline-1-oxide; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine