Cyclin D2 is essential for BCR-mediated proliferation and CD5 B cell development.
Progression into G(1) in B lymphocytes is regulated by cyclins D2 and D3, components of the cell cycle machinery currently believed to have overlapping and potentially redundant roles in cell cycle control. To study the specific role of cyclin D2 in B lymphocyte proliferation, we examined B cells from cyclin D2(-/-) mice and demonstrate a specific requirement for cyclin D2 in BCR- but not CD40- or lipopolysaccharide-induced proliferation. Furthermore, conventional B cell development proceeds normally in the mutant mice; however, the CD5 B cell compartment is dramatically reduced, suggesting that cyclin D2 is important in CD5 B cell development as well as antigen-dependent B cell clonal expansion.
Solvason
N
N
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304, USA.
Wu
W W
WW
Parry
D
D
Mahony
D
D
Lam
E W
EW
Glassford
J
J
Klaus
G G
GG
Sicinski
P
P
Weinberg
R
R
Liu
Y J
YJ
Howard
M
M
Lees
E
E
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D015153:Blotting, Western; D019013:CD40 Antigens / Q000494:pharmacology; D018956:CD5 Antigens / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D056742:Cyclin D2; D056743:Cyclin D3; D016213:Cyclins / Q000032:analysis / Q000172:deficiency / Q000276:immunology*; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000276:immunology; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D015513:Oncogene Proteins / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr
D000906:Antibodies; D019013:CD40 Antigens; D018956:CD5 Antigens; C486196:Ccnd2 protein, mouse; C534697:Ccnd3 protein, mouse; D056742:Cyclin D2; D056743:Cyclin D3; D016213:Cyclins; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D015847:Interleukin-4; D011505:Protein-Tyrosine Kinases; C496614:Bcr protein, mouse; D051562:Proto-Oncogene Proteins c-bcr