[The importance of immunohistochemical examination in the diagnosis of malignant lymphoproliferative diseases--the author's personal experience].
A high-quality approach to lymphoproliferative diseases needs mastering the basic principles of morphology, immunology and genetics. The diagnosis relies on both traditional methods (histopathological analysis) and modern procedures (supported by advanced techniques such as immunohistochemistry, flow cytometry, and molecular biology). This paper presents a personal point of view developed by the author as a consequence of experiencing the immunohistochemical reactions for the diagnosis of non-Hodgkin's malignant lymphomas. Immunohistochemistry, as an effective and currently practiced procedure, allows the identification of the cellular line of the proliferating cells. Full details are given for material and methods, providing a complete characterization of the used monoclonal antibodies (DAKO-CD20, L26, DAKO-CD3, DAKO-CD68, PG-M1, DAKO-E29, EMA), as well as an integral description of the sequenced working phases. Relevance, efficiency, performance, limits and perspectives of the employed procedure are discussed with direct references to the results of this personal study.
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Disciplina de Histologie, Facultatea de Medicină, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
D000911:Antibodies, Monoclonal; D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000175:diagnosis*
D000911:Antibodies, Monoclonal