Transfusion of platelet concentrates cryopreserved with ThromboSol plus low-dose dimethylsulphoxide in patients with severe thrombocytopenia: a pilot study.
We have recently reported the possibility of supporting the phase of severe thrombocytopenia after high-dose chemotherapy (HDC) and stem cell transplantation using 5% dimethylsulphoxide (DMSO)-cryopreserved autologous platelet concentrates (PCs). The aim of the present study was to evaluate the therapeutic potential of ThromboSol (a recently developed platelet storage solution) plus PCs cryopreserved in 2% DMSO in patients undergoing myeloablative chemotherapy and autologous transplantation. PCs were collected from 14 women with breast cancer by a single plateletapheresis and cryopreserved in ThromboSol/2% DMSO by either direct insertion in a -80 degrees C freezer or in liquid nitrogen after computer-controlled rate (CR) freezing. When required, PCs were thawed, centrifuged to remove the cryoprotectants and transfused. In vitro studies on thawed platelets showed loss of epitopes of surface glycoproteins and a marked reduction of functional activity compared with fresh platelets. Transfusion of CR-frozen PCs was associated with a mean 1 h corrected count increment (CCI) of 9.2 +/- 5.4 x 109/l and only one allogeneic PC was required in this group. In contrast, six out of seven patients required additional allogeneic transfusions in the -80 degrees C group (CCI = 2.7 +/- 1.4 x 109/l). ThromboSol-treated PCs have the ability to overcome thrombocytopenia if processed by a CR freezing protocol, but appear ineffective when frozen by direct placing at -80 degrees C.
Pedrazzoli
P
P
Divisione di Oncologia Medica, IRCCS Fondazione 'S. Maugeri', Pavia, Italy.
Noris
P
P
Perotti
C
C
Schiavo
R
R
Ponchio
L
L
Belletti
S
S
Da Prada
G A
GA
Balduini
C L
CL
Salvaneschi
L
L
Robustelli Della Cuna
G
G
Siena
S
S
D001804:Blood Transfusion, Autologous; D001943:Breast Neoplasms / Q000628:therapy*; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D004334:Drug Administration Schedule; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010865:Pilot Projects; D010974:Platelet Aggregation; D017713:Platelet Transfusion / Q000379:methods*; D013921:Thrombocytopenia / Q000628:therapy*
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide