RT-PCR as a tool for systematic transcriptional analysis of large regions of the Bacillus subtilis genome.
Transcriptional analysis of five different regions of the Bacillus subtilis 168 genome, comprising a total of 175 kb encoding newly identified genes, was carried out using the RT-PCR technique as part of the functional analysis of the whole genome of this bacterium. Amplification of mRNA fragments allowed the detection of both highly and poorly transcribed genes covering 81% of putative ORFs, and also the monitoring of variations in the expression level among genes differentially expressed during particular bacterial growth phases.
Hernández
Alicia
A
Centro Nacional de Biotecnologı́a (CSIC), Campus de Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
Figueroa
Angélica
A
Centro Nacional de Biotecnologı́a (CSIC), Campus de Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
Rivas
Luis A
LA
Centro Nacional de Biotecnologı́a (CSIC), Campus de Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
Parro
Vı Ctor
VC
Centro Nacional de Biotecnologı́a (CSIC), Campus de Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
Mellado
Rafael P
RP
Centro Nacional de Biotecnologı́a (CSIC), Campus de Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
D001412:Bacillus subtilis / Q000235:genetics*; D016680:Genome, Bacterial; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014158:Transcription, Genetic