Evidence that hyaluronidase is not involved in tissue invasion of the protozoan parasite Entamoeba histolytica.
As previous reports suggested that a hyaluronidase is involved in tissue invasion of Entamoeba histolytica, we searched for such an activity in trophozoite extracts. A hyaluronidase activity was not detectable in long-term cultures or in amoebae freshly passaged through a gerbil liver, as evidenced by four different techniques.
Nickel
R
R
Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
Stern
R
R
Leippe
M
M
D000818:Animals; D002417:Cattle; D004748:Entamoeba histolytica / Q000472:pathogenicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005782:Gels; D005849:Gerbillinae; D006821:Hyaluronoglucosaminidase / Q000378:metabolism / Q000502:physiology*; D012685:Sepharose; D013194:Staining and Labeling; D013379:Substrate Specificity
D005782:Gels; D012685:Sepharose; D006821:Hyaluronoglucosaminidase