title: Inactivation of bovine herpesvirus 1 in semen using a hyperimmune egg yolk semen extender.
abstract: Hyperimmune egg yolk semen extender was used for the inactivation of bovine herpesvirus (BHV-1) in experimentally infected bovine semen. As much as 5 x 10(4) TCID50/ml of virus was inactivated in semen as assayed by tissue culture. Moreover the hyperimmune egg yolk semen extender did not produce any adverse effect on the quality of the semen after being frozen/thawed in comparison with normal egg yolk semen extender (P > 0.05). The hyperimmune egg yolk semen extender is considered an important tool for containing the spread of BHV-1 from infected semen.
authors.affiliation: Veterinary Faculty of Complutense University, Madrid, Spain. nivsilva@dedalus.lcc.ufmg.br
authors.forename: N
authors.initials: N
authors.lastname: Silva
authors.forename: A
authors.initials: A
authors.lastname: Solana
authors.forename: J M
authors.initials: JM
authors.lastname: Castro
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control / Q000821:virology*; D004530:Egg Yolk; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D012661:Semen / Q000821:virology*; D012662:Semen Preservation / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D007136:Immunoglobulins