[Isoelectric characterization of extracellular polygalacturanases of various Aspergillus alliaceus strains].
The composition of pectin hydrolase complexes produced by various Aspergillus alliaceus strains was studied under the conditions of induction, catabolite repression, or constitutive synthesis. The strains were found similar in terms of the polygalacturonase spectrum and different with regard to the levels of endo- and exoenzyme activities. The analysis of the zymograms of inducible polygalacturonases revealed that all tested cultures contained at least 24 molecular forms of polygalacturonase. Taking into account only the three molecular forms typical of all analyzed strains of A. alliaceus with pI values of 5.7, 5.9, and 6.3, one can use the spectrum of constitutive, catabolite repression-resistant polygalacturonases as an additional taxonomic species criterion.
Mikhaĭlova
R V
RV
Institute of Microbiology, National Academy of Sciences of Belarus, Minsk.
Sapunova
L I
LI
Lobanok
A G
AG
Iasenko
M I
MI
Shishko
Zh F
ZhF
D001230:Aspergillus / Q000378:metabolism*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D011096:Polygalacturonase / Q000737:chemistry* / Q000378:metabolism*
D007527:Isoenzymes; D011096:Polygalacturonase