Citrate utilization by homo- and heterofermentative lactobacilli.
Citrate utilization by several homo- and heterofermentative lactobacilli was determined in Kempler and McKay and in calcium citrate media. The last medium with glucose permitted best to distinguish citrate-fermenting lactobacilli. Lactobacillus rhamnosus ATCC 11443, Lactobacillus zeae ATCC 15820 and Lactobacillus plantarum ATCC 8014 used citrate as sole energy source, whereas in the other strains, glucose and citrate were cometabolized. Some lactobacilli strains produced aroma compounds from citrate. Citrate transport experiments suggested that all strains studied presented a citrate transport system inducible by citrate. The levels of induction were variable between several strains. Dot blot experiment showed that lactobacilli do not present an equivalent plasmid coding for citrate permease.
Medina de Figueroa
R
R
Centro de Referencia para Lactobacilos, Tucumán, Argentina. rfiguer@unt.edu.ar
Alvarez
F
F
Pesce de Ruiz Holgado
A
A
Oliver
G
G
Sesma
F
F
D000093:Acetoin / Q000378:metabolism; D001426:Bacterial Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002951:Citrates / Q000378:metabolism*; D003470:Culture Media; D003931:Diacetyl / Q000378:metabolism; D005285:Fermentation; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D027981:Symporters
D001426:Bacterial Proteins; D002352:Carrier Proteins; C096373:CitP protein, Lactobacillales; D002951:Citrates; D003470:Culture Media; D027981:Symporters; D000093:Acetoin; D005947:Glucose; D003931:Diacetyl