A lectin from an edible mushroom Pleurotus ostreatus as a food intake-suppressing substance.
In an experiment in which rats were allowed free access to food and water, the rats did not eat the diet containing a mushroom Pleurotus ostreatus even if they were emaciated. A P. ostreatus lectin (POL) was isolated from the mushroom as the food intake-suppression principle. In hemagglutination inhibition assays, Me-alphaGalNAc was the most potent inhibitor among the monosaccharides tested. Among all the sugars tested, 2'-fucosyllactose (Fucalpha1-->2Galbeta1-->4Glc) was the strongest inhibitor and its inhibitory potency was five times greater than that of Me-alphaGalNAc. POL exhibited a binding ability to bovine submaxillary mucin (BSM) and asialo-BSM and the other glycoproteins were inert to the binding. The food intake-suppressing activity of POL was dependent on the dose. The diet containing 0.1% POL caused a 50% decrease in the food intake of rats against the control.
Kawagishi
H
H
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan. achkawa@agr.shizuoka.ac.jp
Suzuki
H
H
Watanabe
H
H
Nakamura
H
H
Sekiguchi
T
T
Murata
T
T
Usui
T
T
Sugiyama
K
K
Suganuma
H
H
Inakuma
T
T
Ito
K
K
Hashimoto
Y
Y
Ohnishi-Kameyama
M
M
Nagata
T
T
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001067:Appetite Depressants / Q000302:isolation & purification* / Q000494:pharmacology; D002412:Cations; D002852:Chromatography, Ion Exchange; D004032:Diet; D004435:Eating / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D008670:Metals / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D020076:Pleurotus / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000596:Amino Acids; D001067:Appetite Depressants; D002412:Cations; D037102:Lectins; D008670:Metals; D010936:Plant Extracts