title: Expression of the elastin promoter in novel tissue sites in transgenic mouse embryos.
abstract: We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Furthermore, in general, expression of the transgene showed developmental regulation similar to that of the endogenous gene. However, the precise location of cellular expression could not be determined in this model. To overcome this limitation, we have developed a similar model, but replaced CAT with the reporter enzyme beta-galactosidase. Enzyme activity was readily detected in the transgenic mouse embryos in expected regions of tissue forming elastic fibers, including the dermis and elastic cartilage. Of considerable interest, however, was the novel finding of expression in specific areas of neuroepithelium of the brain and in the perichondrium surrounding areas destined to form hyaline cartilage in endochondral bone formation. These latter areas included all the bones of the limbs, the spine and rib cage. It appeared that these segments of elastin expression demarcated the border between the developing cartilage and the surrounding mesenchymal tissue. Elastin promoter expression was also found in developing somites, in the mesenchymal layer of the forming cornea of the eye, in the genital tubercle and in the epithelium destined to form the olfactory epithelium. These findings indicate that the elastin promoter is activated during embryonic development in a variety of tissues, suggesting that elastin gene expression may play a role in organizing cutaneous, skeletal and neural structures.
authors.affiliation: Department of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, PA 19104, USA.
authors.forename: J
authors.initials: J
authors.lastname: Lakkakorpi
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Decker
authors.forename: E
authors.initials: E
authors.lastname: Korkeela
authors.forename: R
authors.initials: R
authors.lastname: Piddington
authors.forename: W
authors.initials: W
authors.lastname: Abrams
authors.forename: M
authors.initials: M
authors.lastname: Bashir
authors.forename: J
authors.initials: J
authors.lastname: Uitto
authors.forename: J
authors.initials: J
authors.lastname: Rosenbloom
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology; D002356:Cartilage / Q000196:embryology* / Q000378:metabolism; D004547:Elastic Tissue / Q000378:metabolism; D004549:Elastin / Q000096:biosynthesis / Q000235:genetics*; D005314:Embryonic and Fetal Development; D004848:Epithelium / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D004549:Elastin; D001616:beta-Galactosidase