Identification of virulence associated markers in the cell wall of pigeon Streptococcus gallolyticus strains.
The cell wall protein profiles of 56 isolates of Streptococcus gallolyticus of differing virulence for pigeons were compared by SDS-PAGE. Additionally, Western blot analysis was performed on the cell wall proteins of 14 strains using sera of pigeons, experimentally infected with A(+)T1 or A(-)T2 strains of S. gallolyticus. The profile of silver stained gels exhibited a complex array of 20-50 bands ranging from less than 6.5-210kDa. A band with molecular mass of 114kDa was only observed in isolates that belonged to the highly virulent A(+)T1, A(+)T2, A(+)T3 and A(-)T1 culture supernatant groups. A band with a slightly higher molecular mass (115kDa) as well as a 207kDa band were only detected in isolates that belonged to the moderately A(-)T3 or low A(-)T2 virulent culture supernatant groups. The 114 and 115kDa band were recognised by all homologous and heterologous pigeon sera used whereas the 207kDa band was only recognised by sera of pigeons infected with a A(-)T2 strain. These findings may indicate that the 114, 115 and 207kDa bands are useful as additional virulence associated markers for pigeon S. gallolyticus strains.
Vanrobaeys
M
M
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Haesebrouck
F
F
Ducatelle
R
R
De Herdt
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D015415:Biomarkers / Q000737:chemistry; D001715:Bird Diseases / Q000382:microbiology*; D015153:Blotting, Western / Q000662:veterinary; D010856:Columbidae; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D007202:Indicators and Reagents / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D008970:Molecular Weight; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity*; D016985:Streptococcus bovis / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity; D014774:Virulence
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D015415:Biomarkers; D007202:Indicators and Reagents; D007211:Indoles; D009580:Nitroblue Tetrazolium; C035455:5-bromo-4-chloro-3-indoxyl phosphate