title: Identification of highly conserved hydrophobic amino acid motif in deduced amino acid sequence of Elymus sibiricus L. mitochondrial S13 ribosomal protein.
abstract: The nucleotide sequence of mitochondrial ribosomal protein rps13 gene from wild perennial grass Elymus sibiricus is presented. It was determined by the method of PCR amplification with specific oligonucleotide primers and the direct sequencing of the amplification product. The sequence of E. sibiricus mitochondrial gene for S13 predicts a hydrophobic ribosomal protein of 116 amino acids that shows strong similarity to those of wheat (99.7% identity) and maize (98%). The deduced amino acid sequence of S13 protein from E. sibiricus and homologous plant's (Zea mays, Daucus carota, Nicotiana tabacum, Marchantia polymorpha) and nonplant's (Escherichia coli) proteins shows the presence of hydrophobic amino acids' motif -L-X10-L-X10-M-X10-L-X10-L-. Slightly modified it can be found in many other ribosomal proteins. This conserved motif is presumed to be particularly important for association of the ribosomal S13 protein with other proteins in the small subunit of the mitochondrial ribosome.
authors.affiliation: Siberian Branch of Russian Academy of Sciences, Siberian Institute of Plant Physiology and Biochemistry, Irkutsk.
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Konstantinov
authors.forename: E L
authors.initials: EL
authors.lastname: Tauson
authors.forename: D S
authors.initials: DS
authors.lastname: Verbitskiĭ
authors.forename: O Ia
authors.initials: OIa
authors.lastname: Dichenko
authors.forename: A Sh
authors.initials: ASh
authors.lastname: Arziev
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000235:genetics*; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D006109:Poaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D000596:Amino Acids; D004272:DNA, Mitochondrial; D018744:DNA, Plant