title: The link between high-fat meals and postprandial activation of blood coagulation factor VII possibly involves kallikrein.
abstract: Contrary to low-fat meals, high-fat meals are known to cause postprandial factor VII (FVII) activation, but the mechanism is unknown. To study the postprandial FVII activation in detail, 18 young men consumed in randomized order high-fat or low-fat test meals. Fasting and non-fasting blood samples were collected. The high-fat test was associated with an increase in plasma triglyceride and kallikrein concentrations and postprandial FVII activation (p<0.001). Plasma kallikrein was strongly associated with triglycerides in fasting and non-fasting samples (r2=0.74-0.87, p<0.0001), suggesting that triglyceride-rich lipoproteins may activate prokallikrein. Neither plasma triglycerides nor kallikrein and activated FVII were statistically associated. This may suggest that additional factors are involved in the postprandial FVII activation. No clear evidence for a role of tissue factor expression by monocytes, factor XII or insulin in postprandial FVII activation was observed. Tissue factor pathway inhibitor and prothrombin fragment 1+2, a marker of thrombin generation, were not affected postprandially after either the high-fat or the low-fat meals. Our findings indicate that triglyceride-rich lipoproteins activate prokallikrein postprandially, which might form an important initial event in FVII activation after consumption of high-fat meals.
authors.affiliation: Research Department of Human Nutrition and Center for Advanced Food Studies, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark. lfl@kvl.dk
authors.forename: L F
authors.initials: LF
authors.lastname: Larsen
authors.forename: P
authors.initials: P
authors.lastname: Marckmann
authors.forename: E M
authors.initials: EM
authors.lastname: Bladbjerg
authors.forename: P B
authors.initials: PB
authors.lastname: Ostergaard
authors.forename: J
authors.initials: J
authors.lastname: Sidelmann
authors.forename: J
authors.initials: J
authors.lastname: Jespersen
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology*; D001786:Blood Glucose; D004041:Dietary Fats / Q000008:administration & dosage*; D004792:Enzyme Precursors / Q000097:blood; D005167:Factor VII / Q000032:analysis / Q000378:metabolism*; D005174:Factor XII / Q000032:analysis / Q000378:metabolism; D015956:Factor XIIa / Q000032:analysis / Q000378:metabolism; D005215:Fasting; D006801:Humans; D007328:Insulin / Q000097:blood; D007610:Kallikreins / Q000097:blood*; D008297:Male; D009000:Monocytes / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D019518:Postprandial Period; D011516:Prothrombin / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D004792:Enzyme Precursors; D007328:Insulin; D010446:Peptide Fragments; D014280:Triglycerides; C045879:prothrombin fragment 1.2; D005167:Factor VII; D011516:Prothrombin; D005174:Factor XII; D007610:Kallikreins; C052910:prokallikrein, tissue; D015956:Factor XIIa