Cloning and characterization of Entamoeba histolytica antigens recognized by human secretory IgA antibodies.
To identify the Entamoeba histolytica antigens capable of inducing secretory IgA (sIgA) responses in humans, a cDNA library from the strain HM1:IMSS was immuno-screened with saliva from patients with intestinal amebiasis or amebic liver abscess. Clones isolated with sIgA antibodies from patients with intestinal amebiasis corresponded to the known serine-rich protein isoform, a 29 kDa cysteine-rich protein and 1-alpha elongation factor. Clones corresponding to enolase, cyclophilin, ribosomal protein L23a, and an Hsp70 family protein were isolated with sIgA from a patient with amebic liver abscess. A glutamic acid-rich peptide (EhGARP) positive with sIgA from a patient with amebic liver abscess was also isolated; for EhGARP, no homologs were found in the protein databases. The antigens isolated are potentially useful in the development of an oral vaccine or new diagnostic tools for amebiasis.
Carrero
J C
JC
Department of Immunology, Instituto de Investigaciones Biomédicas, UNAM, México DF, México.
Petrossian
P
P
Acosta
E
E
Sánchez-Zerpa
M
M
Ortiz-Ortiz
L
L
Laclette
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004404:Dysentery, Amebic / Q000276:immunology*; D004748:Entamoeba histolytica / Q000145:classification / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D008101:Liver Abscess, Amebic / Q000276:immunology*; D008969:Molecular Sequence Data; D012463:Saliva / Q000469:parasitology
D000953:Antigens, Protozoan; D018076:DNA, Complementary; D007071:Immunoglobulin A, Secretory