title: Monoclonal antibodies to an HIV-1 group O envelope recombinant.
abstract: Monoclonal antibodies were developed to a recombinant HIV-I group O envelope protein derived from the isolate HAM112. These monoclonal antibodies were characterized for reactivity to a series of overlapping synthetic peptides (29-30 mers) covering gp120 C-terminal and gp41 ectodomain regions of the HIV-1 group O envelope protein. Most of these monoclonal antibodies reacted with peptides spanning sequences analogous to HIV-1 group M epitopes identified from studies in mice and humans. However, several of the antibodies that were nonreactive to individual peptides did react to a mixture of longer peptides from the N-terminal and C-terminal helical regions of the gp41 ectodomain. The monoclonal antibodies described in this study are valuable tools for characterization of antigenic differences between HIV-1 group O and group M viruses.
authors.affiliation: Hybridoma Development and Sourcing, Abbott Laboratories, Abbott Park, Illinois 60064, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Scheffel
authors.forename: R
authors.initials: R
authors.lastname: Ziemann
authors.forename: D
authors.initials: D
authors.lastname: Hawksworth
authors.forename: J
authors.initials: J
authors.lastname: Tyner
authors.forename: R K
authors.initials: RK
authors.lastname: Hickman
authors.forename: J
authors.initials: J
authors.lastname: Hackett
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000276:immunology; D015497:HIV-1 / Q000145:classification* / Q000276:immunology*; D007124:Immunoenzyme Techniques; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D011994:Recombinant Proteins