Measurement of the rate of RNA hydrolysis in aqueous solution at elevated temperatures using a new monitoring method for hydrothermal reactions.
A new monitoring method for hydrothermal reactions, which is capable to monitor reactions in aqueous solution at 100-300 degrees C in 0.003-140 s, has been applied for the measurements of the rate of hydrolysis of oligonucleotides containing ribonucleic phosphodiester linkage. The hydrolyses of several types of oligonucleotides were monitored using the method at over 100 degrees C.
Kawamura
K
K
Department of Applied Chemistry, College of Engineering, Osaka Prefecture University, Sakai, Japan.
D001483:Base Sequence; D006358:Hot Temperature; D006868:Hydrolysis; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012996:Solutions; D013329:Structure-Activity Relationship; D014867:Water
D009838:Oligodeoxyribonucleotides; D012996:Solutions; D014867:Water; D012313:RNA