title: Decrease in phorbol ester-induced potentiation of noradrenaline release in synapsin I-deficient mice.
abstract: Synapsin I is involved in regulating amino acid neurotransmitter release, but has a less clear role in noradrenergic nerve terminals. To better understand the role of synapsin I in the function of noradrenergic nerve terminals, we compared noradrenaline release in wild-type and synapsin I-deficient mice. No difference was found in the accumulation or in the Ca(2+)-independent release of [(3)H]noradrenaline in cerebrocortical synaptosomes from wild-type and synapsin I-deficient mice. Synaptosomes lacking synapsin I also displayed no gross alterations in either the time course or the Ca(2+)-dependency of [(3)H]noradrenaline release when stimulated by depolarizing secretagogues or ionophore treatment. In wild-type synaptosomes, activation of protein kinase C by phorbol ester treatment resulted in a Ca(2+)-dependent increase in [(3)H]noradrenaline release evoked by depolarizing secretagogues and ionophore treatment. The phorbol ester-mediated enhancement of [(3)H]noradrenaline release evoked by depolarizing secretagogues, but not by ionophore treatment, was greatly reduced in synapsin I-deficient synaptosomes. These results indicate that synapsin I plays a role in regulating noradrenaline release.
authors.affiliation: Neurochemical Laboratory, Institute of Basic Medical Science, University of Oslo, Oslo, Norway. ivar.walaas@basalmed.uio.no
authors.forename: S I
authors.initials: SI
authors.lastname: Walaas
authors.forename: S
authors.initials: S
authors.lastname: Hilfiker
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: L S
authors.initials: LS
authors.lastname: Chin
authors.forename: P
authors.initials: P
authors.lastname: Greengard
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology*; D051379:Mice; D008817:Mice, Mutant Strains; D009638:Norepinephrine / Q000378:metabolism* / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology*; D016704:Synapsins / Q000172:deficiency*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
chemical_list: D002273:Carcinogens; D010703:Phorbol Esters; D016704:Synapsins; D002118:Calcium; D009638:Norepinephrine