title: Cellular adaptation of the mouse cortical thick ascending limb of Henle's loop (CTAL) to dietary magnesium restriction: enhanced transepithelial Mg2+ and Ca2+ transport.
abstract: Mice aged 4 or 8 weeks were fed with a low-Mg2+ diet for 1, 2, 3 or 4 days. After 1 day of diet, the urinary excretion of Mg2+ and Ca2+ was strongly reduced in both animal groups (4 and 8 weeks), accompanied by a significant fall in plasma Mg2+ concentration and an increase in urinary volume. This profile persisted after 2, 3 or 4 days of dietary Mg2+ restriction. After 1 day of diet, transepithelial ion net fluxes of Na+, Cl-, Ca2+ and Mg2+ (JNa' JCI, JCl, JMg) measured in vitro from isolated perfused cortical thick ascending limbs (CTALs) of these animals remained unchanged. After 2 days of diet, measurements of J(Ca) and J(Mg) in isolated perfused CTALs showed that transepithelial Mg2+ and Ca2+ reabsorption were enhanced in CTALs from Mg(2+)-depleted, 8-week-old animals, whereas transepithelial Mg2+ and Ca2+ transport were not altered in 4-week-old mice. JNa and JCl and the transepithelial potential (PDte) were not modified in CTALs from either animal group. Our results suggest that a low-Mg2+ diet leads to urinary retention of Mg2+ and Ca2+ which is most likely due to increased Mg2+ and Ca2+ transport in the CTAL. Furthermore, in response to dietary Mg2+ restriction, the reabsorption of divalent cations in the CTAL of adult, but not of young, mice undergoes cellular adaptation.
authors.affiliation: Département de Biologie Cellulaire et Moléculaire, CEA Saclay, URA CNRS 1859, Gif-sur Yvette, France. wittner@dsvidf.cea.fr
authors.forename: M
authors.initials: M
authors.lastname: Wittner
authors.forename: S
authors.initials: S
authors.lastname: Jounier
authors.forename: G
authors.initials: G
authors.lastname: Deschênes
authors.forename: C
authors.initials: C
authors.lastname: de Rouffignac
authors.forename: A
authors.initials: A
authors.lastname: Di Stefano
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D004032:Diet; D004326:Drinking / Q000187:drug effects; D004573:Electrolytes / Q000097:blood / Q000378:metabolism / Q000652:urine; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007672:Kidney Cortex; D008138:Loop of Henle / Q000166:cytology / Q000502:physiology*; D008274:Magnesium / Q000008:administration & dosage* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D009318:Natriuresis / Q000187:drug effects; D013997:Time Factors
chemical_list: D004573:Electrolytes; D008274:Magnesium; D002118:Calcium