title: Cytotoxicity of dental resin monomers in the presence of S9 mix enzymes.
abstract: The purpose of the this study was to evaluate the cytotoxicity of dental resin monomers in the presence of a rat liver S9 mix containing cytochrome P 450 enzymes. JTC-12 cells derived from a monkey kidney were seeded on a 96-well multi-well-plate at 9 x 10(3) cells per well. After cultivation, the S9 mix was added to the wells as an S9 mix group (+S9), and PBS- was added to the other wells as a none-S9 mix group (-S9), then 7 different concentrations of various monomers were added to each well. All the specimens were cultured for another 24 hrs. The cell survival ratios (CSR) were calculated by using a neutral red cytotoxicity assay. CSR for 50 micrograms/mL of Bis-GMA/S9 mix was 92.6% while for none-S9 mix it was 6.6%. The values of CSR for UDMA, Bis-MPEPP, EGDMA, TEGDMA, DMAEM, 4-META and HEMA exhibited a reduction in cytotoxicity in the presence of the S9 mix. There were significant differences between +S9 and -S9 for respective monomers (p < 0.05). However, there were no significant differences between +S9 and -S9 for MMA (p < 0.05).
authors.affiliation: Department of Fixed Prosthodontics, School of Dentistry, Health Sciences University of Hokkaido, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hikage
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: C F
authors.initials: CF
authors.lastname: Cox
authors.forename: K
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D001711:Biotransformation; D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival; D003188:Composite Resins / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003764:Dental Materials / Q000633:toxicity*; D007668:Kidney / Q000166:cytology; D008252:Macaca fascicularis; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D018675:Toxicity Tests
chemical_list: D003188:Composite Resins; D003764:Dental Materials; D017438:Bisphenol A-Glycidyl Methacrylate; D003577:Cytochrome P-450 Enzyme System