title: Platelet prothrombinase activity, a final pathway platelet procoagulant activity, is overexpressed in type 1 diabetes: no relationship with mean platelet volume or background retinopathy.
abstract: There is overwhelming evidence that platelets from diabetic individuals are hyperreactive, not only when microvascular complications are apparent, but already at an early stage of the disease. There is still controversy about the question of whether primary hyperreactive platelets may contribute to the origin or progression of microangiopathy or whether diabetic platelet hyperfunctionality is just a logical consequence of a continuous low-grade activation of platelets by contact with a diseased microvascular wall. As a consequence of platelet activation, the outer layer of its phospholipid membrane is more procoagulant than in the quiescent state, stimulating thrombin formation in plasma. This platelet function is called platelet procoagulant activity. We studied platelet prothrombinase activity (PPA), a final pathway platelet procoagulant activity of type 1 diabetic platelets, and looked for an eventual correlation with microvascular disease (background retinopathy) and mean platelet volume (MPV). Stypven clotting times (SCTs), reflecting PPA expression, and MPV of citrated platelet-rich plasma (PRP), were measured in 21 patients with type 1 diabetes-10 with and 11 without background retinopathy-under clinically acceptable metabolic control and compared them to 20 disease-free voluntary controls. We also compared PPA expression and MPV in diabetic individuals with and without retinopathy. With the SCT, a selective test adapted for studying PPA in PRP, we found hyperexpression of PPA in all diabetic patients. We found no difference in MPV between diabetic and control PRP. Comparing patients with and without background retinopathy we found no significant difference in PPA expression. From these results, we suggest that the phospholipid surface of diabetic platelets, more than the surface of normal control platelets, stimulate the expression of PPA. This diabetic platelet coagulant anomaly was not related to an increased platelet mass (higher MPV) nor to the presence of microangiopathy. We conclude that PPA hyperexpression is associated with patients with type 1 diabetes, already occurring in an early stage of the disease, and not necessarily a consequence of early-stage microvascular disease, because the anomaly is also demonstrable, in the same degree, in patients with diabetes without microangiopathy.
authors.affiliation: Laboratory of Hematology and Hemostasis, Antwerp University Hospital, Edegem, Belgium. marc.van.der.planken@uza01.uza.uia.ac.be
authors.forename: M G
authors.initials: MG
authors.lastname: van der Planken
authors.forename: F J
authors.initials: FJ
authors.lastname: Vertessen
authors.forename: J
authors.initials: J
authors.lastname: Vertommen
authors.forename: W
authors.initials: W
authors.lastname: Engelen
authors.forename: Z N
authors.initials: ZN
authors.lastname: Berneman
authors.forename: I
authors.initials: I
authors.lastname: De Leeuw
mesh_terms: D000328:Adult; D000368:Aged; D001777:Blood Coagulation / Q000502:physiology*; D001792:Blood Platelets / Q000201:enzymology / Q000502:physiology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000201:enzymology; D003930:Diabetic Retinopathy / Q000097:blood / Q000201:enzymology; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010976:Platelet Count; D012016:Reference Values; D013925:Thromboplastin / Q000032:analysis*
chemical_list: D006442:Glycated Hemoglobin; D013925:Thromboplastin