Inhibition of apamin-sensitive K+ current by hypoxia in adult rat adrenal chromaffin cells.
The effect of hypoxia on small-conductance Ca(2+)-activated K+ current was investigated in a study of adult rat adrenomedullary chromaffin cells (AMCs), which were maintained in short-term culture. The nystatin-perforated, whole-cell patchclamp technique was used to study the effect of hypoxia with minimum perturbation of the intracellular milieu. Under voltage-clamp conditions, acute hypoxia (P(O2) approximately equal to 25 mmHg) suppressed the whole-cell outward currents of more than half the AMCs (24/46). This suppression was eliminated after application of apamin (400 nM), a selective inhibitor of small-conductance Ca(2+)-activated K+ current (I(SK)(Ca)) (n=5), suggesting that an apamin-sensitive component of whole-cell currents is suppressed during hypoxia. In contrast to I(SK)(Ca), Ca2+ current (I(Ca)) (n=10) was not affected by hypoxia. Finally, under current-clamp conditions, hypoxia reversibly depolarized the resting membrane potential of adult AMCs (34/40). Apamin, however, eliminated the hypoxia-induced depolarization (400 nM) (7/8), suggesting that hypoxic depolarization is related to the suppression of I(SK(Ca). From the above results, we conclude that adult AMCs are sensitive to hypoxia, and that I(SK)(Ca) contributes to the hypoxia-induced suppression of whole-cell outward current and depolarization of the resting membrane potential in adult AMCs.
Lee
J
J
Department of Physiology and Biophysics, Seoul National University College of Medicine, Korea.
Lim
W
W
Eun
S Y
SY
Kim
S J
SJ
Kim
J
J
D000313:Adrenal Medulla / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D001030:Apamin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology; D002478:Cells, Cultured; D019439:Chromaffin Cells / Q000502:physiology*; D004553:Electric Conductivity; D000860:Hypoxia / Q000473:pathology / Q000503:physiopathology*; D008564:Membrane Potentials; D011188:Potassium / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001030:Apamin; D011188:Potassium; D002118:Calcium