Incorporation of two nonnatural amino acids into proteins through extension of the genetic code.
A novel method of the in vitro incorporation of two nonnatural amino acids into proteins through extension of the genetic code was developed. The streptavidin mRNA containing AGGU and CGGG, and chemically aminoacylated tRNA(ACCU) and tRNA(CCCG) were prepared, then they were added into E. coli in vitro protein synthesizing system. As a result, two nonnatural amino acids were successfully incorporated into desired sites of streptavidin.
Hohsaka
T
T
Department of Bioscience and Biotechnology, Okayama University, Japan.
Ashizuka
Y
Y
Sisido
M
M
D001483:Base Sequence; D003062:Codon / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005815:Genetic Code; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019809:Streptavidin / Q000235:genetics*
D003062:Codon; D011506:Proteins; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; D019809:Streptavidin