The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice.
Almost all the nuclear genes of four Gramineae (maize, wheat, barley, rice) and pea are located in DNA fractions covering only a 1-2% GC range and representing between 10 and 25% of the different genomes. These DNA fractions comprise large gene-rich regions (collectively called the 'gene space') separated by vast gene-empty, repeated sequences. In contrast, in Arabidopsis thaliana, genes are distributed in DNA fractions covering an 8% GC range and representing 85% of the genome. Here, we investigated the integration of a transferred DNA (T-DNA) in the genomes of Arabidopsis and rice and found different patterns of integration, which are correlated with the different gene distributions. While T-DNA integrates essentially everywhere in the Arabidopsis genome, integration was detected only in the gene space, namely in the gene-rich, transcriptionally active, regions of the rice genome. The implications of these results for the integration of foreign DNA are discussed.
Barakat
A
A
Laboratoire de Biologie Moléculaire et Physiologie Végétale, Université de Perpignan, 52 Avenue Villeneuve, 66000, Perpignan, France.
Gallois
P
P
Raynal
M
M
Mestre-Ortega
D
D
Sallaud
C
C
Guiderdoni
E
E
Delseny
M
M
Bernardi
G
G
D017360:Arabidopsis / Q000235:genetics*; D001482:Base Composition; D015139:Blotting, Southern; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D016254:Mutagenesis, Insertional / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial; C036483:T-DNA