title: Populations of the south-west Pacific malaria vector Anopheles farauti s.s. revealed by ribosomal DNA transcribed spacer polymorphisms.
abstract: Malaria in the south-west Pacific is transmitted by members of the Anopheles punctulatus group which comprises 12 cryptic species with overlapping morphology. The most widely distributed species of the group is Anopheles farauti s.s. (An. farauti 1) found throughout northern Australia, Papua New Guinea, eastern Indonesia, the Solomon Islands and Vanuatu. A study of the population structure of this species using PCR-RFLP analysis on the ribosomal DNA internal transcribed spacer 1 reveals five genotypes which had distinct geographical distributions. Where these distributions overlap, genotype hybrids can be identified. Heteroduplex analysis of the ITS2 region reveals combinations of nonhomogenized ITS2 sequences and subsequently seven identifiable genotypes, reflecting the ITS1 distribution. Sequence analysis of these ITS2 polymorphisms reveals a minimum of 13 ITS2 sequence types present in heterogeneous combinations in individual mosquitoes. It appears that there are different levels of evolution occurring within the ITS1 and ITS2 regions. These data suggest that An. farauti s.s. may contain multiple loci for the rDNA gene family or that the homogenization of these regions is relatively slow and can be used in genetic studies of population distribution and structure.
authors.affiliation: Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology, Sydney, Gore Hill 2065, Australia. nigel.beebe@uts.edu.au
authors.forename: N W
authors.initials: NW
authors.lastname: Beebe
authors.forename: R D
authors.initials: RD
authors.lastname: Cooper
authors.forename: D H
authors.initials: DH
authors.lastname: Foley
authors.forename: J T
authors.initials: JT
authors.lastname: Ellis
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D005838:Genotype; D008288:Malaria / Q000635:transmission; D008542:Melanesia; D009692:Nucleic Acid Heteroduplexes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D009692:Nucleic Acid Heteroduplexes