The thrombogram: monitoring thrombin generation in platelet-rich plasma.
A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a clot or the presence of platelets and direct measurement in platelet rich plasma is possible.
Hemker
H C
HC
Department of Biochemistry and Synapse BV, Cardiovascular Research Institute (CARIM) Maastricht, The Netherlands. Coen.Hemker@thrombin.com
Giesen
P L
PL
Ramjee
M
M
Wagenvoord
R
R
Béguin
S
S
D019540:Area Under Curve; D001780:Blood Coagulation Tests; D003374:Coumarins / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis*; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009842:Oligopeptides / Q000032:analysis*; D010949:Plasma; D010976:Platelet Count; D013917:Thrombin / Q000032:analysis*
D003374:Coumarins; D005456:Fluorescent Dyes; D009842:Oligopeptides; C411346:benzyloxycarbonyl-glycyl--glycyl-arginine-4-methylcoumaryl-7-amide; D013917:Thrombin