cDNA cloning and expression of a novel estrogen receptor beta-subtype in goldfish (Carassius auratus).
We have isolated a second goldfish estrogen receptor (ER) beta-subtype (gfER-beta2) cDNA which is distinct from the liver-derived ER-beta (gfER-beta1) cDNA reported previously. The 2650-bp cDNA, isolated from a goldfish pituitary and brain cDNA library, encodes a 610 amino acid (aa) protein which shows only a 53% aa sequence identity with gfER-beta1 in overall structure. RT-PCR analysis showed that mRNA of gfER-beta2, in contrast to that of gfER-beta1, was predominantly expressed in pituitary, telencephalon and hypothalamus as well as in liver of female goldfish. The existence of a second distinct ER-beta subtype opens new dimensions for studying tissue-specific regulation of gene expression by estrogen in the tetraploid goldfish.
Ma
C H
CH
Department of Zoology, The University of Hong Kong, China.
Dong
K W
KW
Yu
K L
KL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D047629:Estrogen Receptor beta; D005260:Female; D015723:Gene Library; D006054:Goldfish / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010902:Pituitary Gland / Q000378:metabolism; D012313:RNA / Q000302:isolation & purification; D011960:Receptors, Estrogen / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D047629:Estrogen Receptor beta; D011960:Receptors, Estrogen; D012313:RNA