Ultrastructural localization of activated NF-kappaB in granule cells of the rat fascia dentata.
The distribution of activated NF-kappaB (p65) was studied in granule cells of the rat fascia dentata using confocal laser scanning microscopy and preembedding immunogold electron microscopy. Activated NF-kappaB, detected with a monoclonal antibody specific for the nuclear localization signal-epitope, was found in proximal dendrites, somata, and nuclei but not in axon terminals of granule cells. Within the nuclei of granule cells, clusters of NF-kappaB immunoreactivity were detected. These data are in line with the proposed function of NF-kappaB as a retrograde signal transducer which transports information from synaptic sites to the nucleus to initiate gene expression.
Kaltschmidt
B
B
Molecular Neurobiology Laboratory, Albert-Ludwigs-University, Freiburg, Germany.
Deller
T
T
Frotscher
M
M
Kaltschmidt
C
C
D000818:Animals; D000918:Antibody Specificity; D018891:Dentate Gyrus / Q000378:metabolism* / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D016328:NF-kappa B / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure*; D051381:Rats
D016328:NF-kappa B