Positioning of region 4 of the Escherichia coli RNA polymerase sigma(70) subunit by a transcription activator.
A DNA cleavage reagent, specifically tethered to residue 581 of the Escherichia coli RNA polymerase sigma(70) subunit, has been used to investigate the location of sigma(70) region 4 in different complexes at the galp(1) promoter and the effect of the cyclic AMP receptor protein. The positions of DNA cleavage by the reagent are not affected by the cyclic AMP receptor protein. We conclude that transcription activation at the galp(1) promoter by the cyclic AMP receptor protein does not involve major conformation changes in or repositioning of sigma(70) region 4.
Bown
J A
JA
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
Kolb
A
A
Meares
C F
CF
Ishihama
A
A
Minchin
S D
SD
Busby
S J
SJ
D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism
D001426:Bacterial Proteins; D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D012808:Sigma Factor; D015534:Trans-Activators; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases