title: Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase.
abstract: A full-length cDNA encoding a novel protein was isolated and sequenced from a human hepatocellular cDNA library. This cDNA consists of 7037 base pairs and has a predicted open reading frame encoding 1924 amino acids. It possesses an RNA-helicase motif containing a DEXH-box in its amino-terminus and an RNase motif in the carboxy-terminus. From a striking homology to Caenorhabditis elegans K12H4.8, it might be a human homolog of the K12H4.8. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 14q31 near the marker D14S605.
authors.affiliation: Department of Molecular Pathogenesis Nagoya University School of Medicine, Japan. smatsuda@tsuru.med.nagoya.u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Ichigotani
authors.forename: T
authors.initials: T
authors.lastname: Okuda
authors.forename: T
authors.initials: T
authors.lastname: Irimura
authors.forename: S
authors.initials: S
authors.lastname: Nakatsugawa
authors.forename: M
authors.initials: M
authors.lastname: Hamaguchi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D004722:Endoribonucleases; D006801:Humans; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics*; D043244:Ribonuclease III; D016415:Sequence Alignment
chemical_list: D004722:Endoribonucleases; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases