title: Hybrid scanning ion conductance and scanning near-field optical microscopy for the study of living cells.
abstract: We have developed a hybrid scanning ion conductance and scanning near-field optical microscope for the study of living cells. The technique allows quantitative, high-resolution characterization of the cell surface and the simultaneous recording of topographic and optical images. A particular feature of the method is a reliable mechanism to control the distance between the probe and the sample in physiological buffer. We demonstrate this new method by recording near-field images of living cells (cardiac myocytes).
authors.affiliation: Division of Medicine, Imperial College School of Medicine, MRC Clinical Sciences Centre, Hammersmith Campus, London W12 0NN, United Kingdom. Y.Korchev@ic.ac.uk
authors.forename: Y E
authors.initials: YE
authors.lastname: Korchev
authors.forename: M
authors.initials: M
authors.lastname: Raval
authors.forename: M J
authors.initials: MJ
authors.lastname: Lab
authors.forename: J
authors.initials: J
authors.lastname: Gorelik
authors.forename: C R
authors.initials: CR
authors.lastname: Edwards
authors.forename: T
authors.initials: T
authors.lastname: Rayment
authors.forename: D
authors.initials: D
authors.lastname: Klenerman
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D066298:In Vitro Techniques; D007477:Ions; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009206:Myocardium / Q000648:ultrastructure; D011817:Rabbits
chemical_list: D007477:Ions