Karyotype and chromosome location of characteristic tandem repeats in the pufferfish Tetraodon nigroviridis.
Karyotype analysis of Tetraodon nigroviridis, a pufferfish of the family Tetraodontidae with a small compact genome (385 Mb) which is currently being investigated in our laboratory, indicates that this species has 2n = 42 chromosomes. The small chromosome size (the largest pair measuring less than 3 microm) has complicated accurate chromosome pairing based on morphology alone. DAPI staining, however, provides a banding-like pattern. Because of quantitative variations of some heterochromatin classes, the chromosome formula can not be established precisely, but is estimated to include approximately 20 meta- or submetacentric chromosomes and 22 subtelocentric chromosomes. A centromeric satellite, telomeric repeats, and the major and minor rRNA clusters have been localized unequivocally by FISH. As a result, the 28S and 5S rDNA sequences can be used as chromosome-specific probes.
Fischer
C
C
Genoscope/Centre National de Séquençage, Evry, France. fischer@genoscope.cns.fr
Ozouf-Costaz
C
C
Roest Crollius
H
H
Dasilva
C
C
Jaillon
O
O
Bouneau
L
L
Bonillo
C
C
Weissenbach
J
J
Bernot
A
A
D000818:Animals; D002503:Centromere / Q000235:genetics; D002871:Chromosome Banding / Q000379:methods; D015342:DNA Probes / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D005260:Female; D005399:Fishes / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006570:Heterochromatin / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles / Q000378:metabolism; D007621:Karyotyping / Q000379:methods; D008297:Male; D009697:Nucleolus Organizer Region / Q000235:genetics; D020161:Physical Chromosome Mapping / Q000379:methods*; D011110:Polymorphism, Genetic / Q000235:genetics; D020080:Tandem Repeat Sequences / Q000235:genetics*; D016615:Telomere / Q000235:genetics
D015342:DNA Probes; D004275:DNA, Ribosomal; D004276:DNA, Satellite; D005819:Genetic Markers; D006570:Heterochromatin; D007211:Indoles; C007293:DAPI