Localization of new, microdissection- generated, anonymous markers and of the genes Pcsk1, Dhfr, Ndub13, and Ccnb1 to rat chromosome region 2q1.
The centromeric region of rat chromosome 2 (2q1) harbors unidentified quantitative trait loci of genes that control tumor growth or development. To improve the mapping of this chromosome region, we microdissected it and generated 10 new microsatellite markers, which we included in the linkage map and/or radiation hybrid map of 2q1, together with other known markers, including four genes: Pcsk1 (protein convertase 1), Dhfr (dihydrofolate reductase), Ndub13 (NADH ubiquinone oxidoreductase subunit b13), and Ccnb1 (cyclin B1). To generate anchor points between the different maps, the gene Ndub13 and the microsatellite markers D2Ulb25 and D2Mit1 were also localized cytogenetically. The radiation map generated in region 2q1 extends its centromeric end of about 150 cR.
Quan
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Université Libre de Bruxelles, IBMM, Laboratoire de Biologie du Développement, Gosselies, Belgium.
Laes
J F
JF
Ravoet
M
M
Van Vooren
P
P
Szpirer
J
J
Szpirer
C
C
D000818:Animals; D002503:Centromere / Q000235:genetics; D019926:Cyclin B / Q000235:genetics*; D056744:Cyclin B1; D042967:Electron Transport Complex I; D008040:Genetic Linkage / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D015674:Mammary Neoplasms, Animal / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D020161:Physical Chromosome Mapping; D010911:Pituitary Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D043484:Proprotein Convertases; D019655:Quantitative Trait, Heritable; D051381:Rats; D029701:Saccharomyces cerevisiae Proteins; D013381:Subtilisins / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
C534701:Ccnb1 protein, rat; D019926:Cyclin B; D056744:Cyclin B1; D029701:Saccharomyces cerevisiae Proteins; D013762:Tetrahydrofolate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D043484:Proprotein Convertases; D013381:Subtilisins; C058751:KEX2 protein, S cerevisiae; D042967:Electron Transport Complex I