Evaluation of recombinant chitinase antigen in serological diagnosis and surveillance of lymphatic filariasis.
Apply recombinant chitinase fusion protein antigen, enzyme-linked immunosorbent assays examined anti-filarial antibodies and evaluated of useful value in serological diagnosis and surveillance of lymphatic filariasis. The test jirds were immunized and infected by chitinase and B. malayi third stage larvae respectively. Functional protein molecular of chitinase was analyzed by SDS-PAGE, Western blot. The result shown that jirds from microfilaremia (mf) and donors with Mf were directly to react with chitinase antigen that positive rate was 100%, but Mf-xt antigen was only 80%. Normal jirds and persons sera from unepidemic control donors all were negative. False positives of 5% and 20% reacted with chitinase and Mf-xt antigens respectively. The results indicate that recombinant chitinase antigen is suitable for detection of active occult or patent lymphatic filariasis with daytime blood samples in residents of endemic areas, is easy to be performed and inexpensive.
Wang
S
S
Guizhou Provincial Institute of Parasitic Diseases, People's Republic of China.
Xiong
M
M
Liu
T
T
Tang
L
L
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D015153:Blotting, Western; D017178:Brugia malayi / Q000201:enzymology / Q000276:immunology; D002688:Chitinases / Q000276:immunology*; D004605:Elephantiasis, Filarial / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005849:Gerbillinae; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000276:immunology
D000947:Antigens, Helminth; D011993:Recombinant Fusion Proteins; D002688:Chitinases