Serotyping of Flavobacterium meningosepticum by co-agglutination method.
The purpose of this investigation was to evaluate the usefulness of a co-agglutination procedure for the typing of Flavobacterium meningosepticum. The sensitivity and specificity of the co-agglutination test was compared to the slide agglutination test using reference strains of the bacterial species. Antisera were characterized by both technics to determine their titer and working dilution. The specificity of the sera was assessed by performing tests which include strains of other species and serotypes. A collection of 47 strains of F. meningosepticum isolated from clinical specimens were typed by both co-agglutination and slide agglutination methods. Co-agglutination proved to be markedly more specific than the slide procedure although both methods were similar in sensitivity. It was concluded that co-agglutination proved to be an excellent method for the serotyping of F. meningosepticum.
Salasawati
H
H
Department of Medical Microbiology and Immunology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.
Ramelah
M
M
Pitt
T L
TL
Holmes
B
B
D000372:Agglutination Tests / Q000379:methods*; D005417:Flavobacterium / Q000145:classification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D008581:Meningitis / Q000382:microbiology; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*