cDNA cloning of rat prolyl oligopeptidase and its expression in the ovary during the estrous cycle.
A cDNA for rat prolyl oligopeptidase was cloned which contained an open reading frame of 2,130 nucleotides encoding a protein of 710 amino acids. The deduced amino acid sequence is around 95% homologous to other mammalian prolyl oligopeptidases and about 40% to bacterial prolyl oligopeptidases. The recombinant prolyl oligopeptidase generated in E. coli was purified and its properties were examined. The substrate specificity and the susceptibility to proteinase inhibitors were similar to those of the native enzyme. Northern blot analysis showed wide expression of the prolyl oligopeptidase gene. Using ovaries from hormone-treated rats, it was found that both the mRNA expression and enzyme activity increased in the luteal phase. These findings suggest the involvement of prolyl oligopeptidase in events associated with corpus luteum formation and/or luteal regression.
Kimura
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A
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
Takahashi
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T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004971:Estrus / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008969:Molecular Sequence Data; D010053:Ovary / Q000187:drug effects / Q000201:enzymology*; D000085822:Prolyl Oligopeptidases; D011480:Protease Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism
D006063:Chorionic Gonadotropin; D017931:DNA Primers; D018076:DNA, Complementary; D006064:Gonadotropins, Equine; D011480:Protease Inhibitors; D012333:RNA, Messenger; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases