Neutrophil intracellular pH and phagocytosis after thermal trauma.
Severe burn trauma induces an acquired dysfunction of neutrophil granulocytes. As neutrophil function is considerably influenced by intracellular pH (pH(i)), the pH(i) of blood neutrophils was longitudinally determined in 19 patients with major burns. pH(i) was measured by a flow cytometric method using the pH-sensitive fluoroprobe carboxy-semi-naphthorhodafluor-1; mechanisms influencing the pH(i) were examined by addition of amiloride (inhibition of Na(+)/H(+) countertransport), diphenylene iodonium (inhibition of NADPH oxidase) and N-formyl-methionyl-leucyl-phenylalanine (activation of H(+) extrusion). The neutrophil phagocytic activity was measured in parallel. Patients showed distinct alterations of neutrophil pH(i), depending on whether they developed sepsis in the postburn period or not. In the sepsis patients pH(i) did not deviate from the values found in healthy volunteers in the first days after injury, but rose afterwards, with significant intracellular alkalinization in the second postburn week (P<0.05). In contrast, patients without sepsis had increased pH(i) in the first (P<0.01 at days 1-2), but not in the second week after burn trauma. Inhibition studies showed that postburn intracellular alkalinization is not solely caused by activation of Na(+)/H(+) countertransport. A clear relation between pH(i) changes and phagocytosis could not be established.
Sachse
C
C
Department of Clinical Chemistry II, Medical School Hannover, Podbielskistrasse 380, 30659, Hannover, Germany. alisia.sachse@t-online.de
Wolterink
G
G
Pallua
N
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D002056:Burns / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis