Quantitative study of beta1-integrin expression and fibronectin interaction profile of T lymphocytes in vitro infected with HIV.
Cell-extracellular matrix interactions, regulated in part by beta1-integrins, play a key role in the recirculation of T lymphocytes and tissue infiltration in inflammatory and immune responses. HIV infection may affect CD4+ T cell adhesion, and the trafficking and migration of these cells, which are crucial for foreign antigen recognition. We investigated this by studying the expression of the beta1-integrin chains CD29 and CD49c, -d, -e, and -f, on in vitro HIV-infected primary T cells. We also assessed fibronectin binding and production by CD4+ lymphocytes. X4 (HIV-1/LAI), R5 (HIV-1/Ba-L), and X4R5 (HIV-2/ROD) strains, and X4R5 primary isolates (HIV-1/DAS, HIV-1/THI), with different cytopathogenicity and replication kinetics, were used. Beta1-integrin expression on CD4+ and CD4- T cell subpopulations was regulated by cell activation with phytohemagglutinin-P and interleukin 2, but was unaffected by HIV infection, even at the peak of viral replication and CD4+ cell depletion. Similarly, fibronectin binding to CD4+ lymphocytes was not affected by HIV infection. This suggests that infected lymphocytes may be able to extravasate, migrate, and recirculate within the body until their death.
Leblond
V
V
CEA, Service de Neurovirologie, DSV/DRM, CRSSA, IPSC, Fontenay aux Roses, France.
Legendre
C
C
Gras
G
G
Dereuddre-Bosquet
N
N
Lafuma
C
C
Dormont
D
D
D001782:Blood Donors; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000821:virology; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D005353:Fibronectins / Q000378:metabolism*; D006678:HIV; D019012:Integrin beta1 / Q000032:analysis / Q000378:metabolism*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D013997:Time Factors; D014779:Virus Replication
D005353:Fibronectins; D019012:Integrin beta1; D007376:Interleukin-2; D010835:Phytohemagglutinins; C028784:phytohemagglutinin-P