Structure of the O polysaccharide and serological classification of Pseudomonas syringae pv. ribicola NCPPB 1010.
The O polysaccharide (OPS) moiety of the lipopolysaccharide (LPS) of a phytopathogenic bacterium Pseudomonas syringae pv. ribicola NCPPB 1010 was studied by sugar and methylation analyses, Smith degradation, and 1H- and 13C-NMR spectroscopy, including 2D COSY, TOCSY, NOESY and H-detected 1H,13C HMQC experiments. The OPS structure was elucidated, and shown to be composed of branched pentasaccharide repeating units (O repeats) of two types, major (1) and minor (2), differing in the position of substitution of one of the rhamnose residues. Both O repeats form structurally homogeneous blocks within the same polysaccharide molecule. Although P. syringae pv. ribicola NCPPB 1010 demonstrates genetic relatedness and similarity in the OPS chemical structure to some other P. syringae pathovars, it did not cross-react with any OPS-specific mAbs produced against heterologous P. syringae strains. Therefore, we propose to classify P. syringae pv. ribicola NCPPB 1010 in a new serogroup, O8.
Ovod
V V
VV
Institute of Medical Technology, University of Tampere, Finland.
Zdorovenko
E L
EL
Shashkov
A S
AS
Kocharova
N A
NA
Knirel
Y A
YA
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry* / Q000276:immunology / Q000472:pathogenicity; D012703:Serotyping
D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens; C095976:O antigen, Pseudomonas; D011135:Polysaccharides, Bacterial