title: [On the mechanism of autotrophic fixation of carbone dioxide by Chloroflexus aurantiacus].
abstract: The activity of two carboxylating enzymes was studied in the green filamentous bacterium Chloroflexus aurantiacus. The carboxylation reaction involving pyruvate synthase was optimized using 14CO2 and cell extracts. Pyruvate synthase was shown to be absent from cells of Cfl. aurantiacus OK-70 and present (in a quantity sufficient to account for autotrophic growth) in cells of Cfl. aurantiacus B-3. Differences in the levels of acetyl CoA carboxylase activity were revealed between cells of the strains studied grown under different conditions. The data obtained confirm the operation of different mechanisms of autotrophic CO2 assimilation in Cfl. aurantiacus B-3 and Cfl. aurantiacus OK-70: in the former organism, it is the reductive cycle of dicarboxylic acids, and in the latter one, it is the 3-hydroxy-propionate cycle.
authors.affiliation: Microbiology Department, Biological Faculty, Moscow State University, Vorob'evy gory, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Ugol'kova
authors.forename: R N
authors.initials: RN
authors.lastname: Ivanovskiĭ
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism*; D019414:Chlorobi / Q000378:metabolism*
chemical_list: D002245:Carbon Dioxide