title: Nucleic acid synthesis for investigations of gene functions.
abstract: The chemical syntheses of genes encoding human c-Ha-ras and T4 endonuclease V are described. These genes have been used for studies of mutagenesis by damaged bases and recognition of nucleic acids by proteins. The modes of interaction of photo-damaged DNA and cognate antibodies have also been investigated by biochemical and physicochemical approaches.
authors.affiliation: Health Sciences University of Hokkaido, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
mesh_terms: D004249:DNA Damage; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000235:genetics*; D005813:Genes, Synthetic; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D014764:Viral Proteins
chemical_list: D014764:Viral Proteins; D004706:Endodeoxyribonucleases; C470698:endonuclease V, phage T4; D043224:Deoxyribonuclease (Pyrimidine Dimer); C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)