title: Reversal of established rat crescentic glomerulonephritis by blockade of macrophage migration inhibitory factor (MIF): potential role of MIF in regulating glucocorticoid production.
abstract: Macrophage migration inhibitory factor (MIF) is a potent pro-inflammatory cytokine that also counter-regulates glucocorticoid action. We investigated whether immunoneutralization of MIF could reverse established experimental crescentic glomerulonephritis and if this treatment could modulate endogenous glucocorticoid levels. Accelerated anti-GBM glomerulonephritis was induced in six littermate pairs of rats. Once crescentic disease was established on day 7, one animal in each pair was given a daily injection of neutralizing anti-MIF antibody (Ab) or irrelevant isotype control Ab for 14 days and then killed on day 21. In addition, a group of 6 animals was killed on day 7 of disease without any treatment. Animals receiving the control Ab exhibited a rapidly progressive glomerulonephritis with severe renal injury (proteinuria), loss of renal function (creatinine clearance), anemia, and marked histologic damage (including glomerular crescent formation), compared with animals killed on day 7 without treatment. In contrast, anti-MIF Ab treatment partially reversed the disease by restoring normal renal function and reducing histological damage compared with untreated animals killed on day 7 (p < 0.05). Interestingly, anti-MIF Ab treatment also prevented severe anemia (p < 0.05). Reversal of disease was associated with a significant reduction in leukocyte infiltration and activation and renal interleukin-1 (IL-1) production. Importantly, anti-MIF Ab treatment caused a significant increase in endogenous serum corticosterone levels, which correlated with the reversal of disease parameters. In conclusion, this study has demonstrated that blocking MIF activity can partially reverse established crescentic glomerulonephritis and suggests that MIF operates by both enhancing the cellular immune response and suppressing the endogenous anti-inflammatory glucocorticoid response.
authors.affiliation: The First Hospital, Sun Yat-Sen University of Medical Sciences, Ghongzhou, China.
authors.forename: N
authors.initials: N
authors.lastname: Yang
authors.forename: D J
authors.initials: DJ
authors.lastname: Nikolic-Paterson
authors.forename: Y Y
authors.initials: YY
authors.lastname: Ng
authors.forename: W
authors.initials: W
authors.lastname: Mu
authors.forename: C
authors.initials: C
authors.lastname: Metz
authors.forename: M
authors.initials: M
authors.lastname: Bacher
authors.forename: A
authors.initials: A
authors.lastname: Meinhardt
authors.forename: R
authors.initials: R
authors.lastname: Bucala
authors.forename: R C
authors.initials: RC
authors.lastname: Atkins
authors.forename: H Y
authors.initials: HY
authors.lastname: Lan
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D003345:Corticosterone / Q000097:blood; D004906:Erythrocyte Count / Q000187:drug effects; D005921:Glomerulonephritis / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D005938:Glucocorticoids / Q000097:blood*; D006967:Hypersensitivity / Q000276:immunology; D007668:Kidney / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007962:Leukocytes / Q000187:drug effects; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000906:Antibodies; D005938:Glucocorticoids; D008263:Macrophage Migration-Inhibitory Factors; D003345:Corticosterone