[Extracellular proteinases and chitinases, produced by a Streptomyces kurssanovii culture].
Extracellular enzymes--a chitinase and a protease with molecular weights 22 and 32 kDa, respectively--were isolated from Streptomyces kurssanovii cells. After purification on modified regenerated chitin, the enzymes were virtually homogeneous according to denaturing PAGE. Both enzymes were found to degrade chitosan.
Il'ina
A V
AV
Bioengineering Center, Russian Academy of Sciences, Moscow, Russia.
Tatarinova
N Iu
NIu
Tikhonov
V E
VE
Varlamov
V P
VP
D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification* / Q000378:metabolism; D005110:Extracellular Space / Q000201:enzymology; D006868:Hydrolysis; D008970:Molecular Weight; D013302:Streptomyces / Q000201:enzymology*
D002686:Chitin; D002688:Chitinases; D010450:Endopeptidases