Decreased insulin-like growth factor-I receptor sites on circulating mononuclear cells from children with acute leukemia.
Insulin-like growth factor-I (IGF-I) is a known mitogen for various cell types, including those of the hematopoietic cell system. To study the role of IGF-I in the neoplastic process of leukemia in children, the authors have determined the number of IGF-I binding sites on circulating mononuclear cells of children with acute leukemia as compared to normal children, using binding assays. The IGF-I binding sites per cell on peripheral mononuclear cells of children with leukemia decreased compared to those of the control group (411 +/- 73 and 1334 +/- 227, respectively, p < .001), while their affinity increased (Kd = 0.14 +/- 0.04 and 0.43 +/- 0.16, respectively, p = .05). Furthermore, in the patients, the number of the IGF-I binding sites was significantly lower in the subgroup of the peripheral mononuclear cells, which included lymphocytes and monocytes, as compared to their number in the peripheral blast cells (254 +/- 43.6 and 536 +/- 98.6, respectively, p = .02). A significant reduction was found in serum GHBP levels in the patients as compared to the controls (28.21 +/- 1.93 and 35.83 +/- 2.90, respectively, p = .02), while serum IGF-I and growth hormone levels were similar in patient and control groups. These results suggest a possible involvement of IGF-I in childhood acute leukemia, but further studies are needed to establish whether IGF-I plays a role in this disease.
Eshet
R
R
Laboratory of Endocrine Research, Felsenstein Medical Research Center, Petah Tikva, Israel.
Silbergeld
A
A
Zaizov
R
R
Stark
B
B
Freud
E
E
Laron
Z
Z
Shamai
Y
Y
D000293:Adolescent; D001752:Blast Crisis / Q000097:blood; D002051:Burkitt Lymphoma / Q000097:blood* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000473:pathology; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D017526:Receptor, IGF Type 1 / Q000097:blood*; D011994:Recombinant Proteins / Q000097:blood; D012016:Reference Values
D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1