title: An NF-Y binding site is important for basal, but not gonadotropin-releasing hormone-stimulated, expression of the luteinizing hormone beta subunit gene.
abstract: Regulated synthesis of luteinizing hormone (LH) requires coordinated transcriptional control of the alpha and LHbeta subunits in pituitary gonadotropes. Several cis-acting elements and trans-acting factors have been defined for control of the LHbeta promoter through heterologous cell culture models. In this report, we describe the identification of bipartite NF-Y (CBF/CP1) binding sites within the proximal bovine LHbeta promoter. When multimerized, one of these sites activates the heterologous, minimal HSV thymidine kinase promoter in the gonadotrope-derived cell line alphaT3-1. The functional role of the promoter-distal site in regulating the full-length bovine LHbeta promoter was assessed in vivo using transgenic mice harboring a mutant promoter linked to the chloramphenicol acetyltransferase reporter gene. While this element is important for conferring high level activity of the LHbeta promoter in pituitary, it does not appear to be essential for mediating gonadotropin-releasing hormone (GnRH) regulation. This is the first characterization of a cis-acting element within this GnRH-dependent promoter that is restricted to regulating basal expression and not GnRH-induced activity.
authors.affiliation: Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Keri
authors.forename: D J
authors.initials: DJ
authors.lastname: Bachmann
authors.forename: A
authors.initials: A
authors.lastname: Behrooz
authors.forename: B D
authors.initials: BD
authors.lastname: Herr
authors.forename: R K
authors.initials: RK
authors.lastname: Ameduri
authors.forename: C C
authors.initials: CC
authors.lastname: Quirk
authors.forename: J H
authors.initials: JH
authors.lastname: Nilson
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D017124:Conserved Sequence; D005260:Female; D005786:Gene Expression Regulation; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006801:Humans; D007986:Luteinizing Hormone / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D010902:Pituitary Gland / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D013937:Thymidine Kinase