title: Localization of phytase in Selenomonas ruminantium and Mitsuokella multiacidus by transmission electron microscopy.
abstract: The localization of phytase (myo-inositol-hexaphosphate phosphohydrolase) in the ruminal bacteria, Selenomonas ruminantium JY35 and Mitsuokella multiacidus 46/5(2), was determined with transmission electron microscopy. Phosphate produced from the enzymatic dephosphorylation of the calcium salt of phytic acid is precipitated as calcium phosphate. The calcium is then replaced with lead to produce electron-dense lead phosphate. This deposition of lead phosphate localized phytase in S. ruminantium JY35 and M. multiacidus 46/5(2) to the outer membrane, and confirmed intracellular expression of the enzyme in Escherichia coli pSrP.2, the recombinant clone which possesses the gene (phyA) encoding phytase (phyA) in S. ruminantium.
authors.affiliation: Agriculture and Agri-Food Canada Research Centre, Lethbridge, AB, Canada.
authors.forename: C G
authors.initials: CG
authors.lastname: D'Silva
authors.forename: H D
authors.initials: HD
authors.lastname: Bae
authors.forename: L J
authors.initials: LJ
authors.lastname: Yanke
authors.forename: K J
authors.initials: KJ
authors.lastname: Cheng
authors.forename: L B
authors.initials: LB
authors.lastname: Selinger
mesh_terms: D010832:6-Phytase / Q000032:analysis*; D000818:Animals; D001419:Bacteria / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D012417:Rumen / Q000382:microbiology*; D020357:Selenomonas / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D010832:6-Phytase