title: Highly sensitive detection of target gene by electrochemical method.
abstract: A highly sensitive DNA sensing method was developed using electrochemically active ligand. This method is based on the detection of electric current generated by electrochemically active ligand concentrated on the electrode. Electrochemically active, intercalating ligand can bind to the double stranded DNA of target gene sequence on the electrode, where the complementary single strand is immobilized as a probe. We succeeded in the detection of 0.1 amol target gene. The technique was applicable to the detection of 0.1-10 amol gene.
authors.affiliation: Department of Chemical Systems and Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: S
authors.initials: S
authors.lastname: Takenaka
authors.forename: M
authors.initials: M
authors.lastname: Takagi
mesh_terms: D004247:DNA / Q000032:analysis* / Q000235:genetics*; D015342:DNA Probes; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005796:Genes; D006046:Gold; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; C027903:oligo (dT); C030570:oligodeoxyadenylic acid; D006046:Gold; D004247:DNA