Genetic polymorphism of the beta-2 adrenergic receptor in atopic and non-atopic subjects.
To investigate a possible genetic basis for reported differences in beta-2 receptor expression in atopic subjects, DNA from 42 atopic children (22 asthmatics and 22 with allergic rhinitis) and 30 non-atopic subjects was Southern blotted and Ban-1 restriction fragment polymorphisms (RFLPS) were studied using a 2.6 kb probe of the human beta-2 receptor gene. Two alleles 3.1 kb and 2.9 kb were identified. Homozygotes and heterozygotes for the two alleles were found with equal frequency in the atopic patients who had asthma and in those who had allergic rhinitis only. The gene frequencies for the upper and lower alleles were 0.45 and 0.55 respectively. Our studies do not provide evidence for an association between a particular polymorphic form of the human beta-2 receptor gene and atopy.
Potter
P C
PC
Department of Clinical Science and Immunology, University of Cape Town, South Africa.
Van Wyk
L
L
Martin
M
M
Lentes
K U
KU
Dowdle
E B
EB
D000328:Adult; D000483:Alleles; D001249:Asthma / Q000235:genetics; D015139:Blotting, Southern; D002648:Child; D004252:DNA Mutational Analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics* / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics; D006255:Rhinitis, Allergic, Seasonal / Q000235:genetics
D018343:Receptors, Adrenergic, beta-2; C051612:endodeoxyribonuclease BanI; D015252:Deoxyribonucleases, Type II Site-Specific