Nitration of manganese superoxide dismutase in cerebrospinal fluids is a marker for peroxynitrite-mediated oxidative stress in neurodegenerative diseases.
Peroxynitrite can nitrate tyrosine residues of proteins. We examined nitrotyrosine-containing proteins in cerebrospinal fluid of 66 patients with neurogenic disease by immunoblot analysis. Nitrated tyrosine residue-containing protein was observed in the cerebrospinal fluid and was concluded to be manganese superoxide dismutase (Mn-SOD). The nitrated Mn-SOD level was strikingly elevated in amyotrophic lateral sclerosis patients and was slightly increased in Alzheimer's and Parkinson's disease patients, whereas an elevated Mn-SOD level was observed only in progressive supranuclear palsy group.
Aoyama
K
K
Department of Internal Medicine III, Shimane Medical University, Izumo, Japan.
Matsubara
K
K
Fujikawa
Y
Y
Nagahiro
Y
Y
Shimizu
K
K
Umegae
N
N
Hayase
N
N
Shiono
H
H
Kobayashi
S
S
D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid / Q000201:enzymology*; D000906:Antibodies; D015415:Biomarkers; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D010300:Parkinson Disease / Q000134:cerebrospinal fluid / Q000201:enzymology*; D011233:Precipitin Tests; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D015415:Biomarkers; D009566:Nitrates; C039642:peroxynitric acid; C002744:3-nitrotyrosine; D014443:Tyrosine; D013482:Superoxide Dismutase; D009584:Nitrogen