title: Infection of bovine immunodeficiency virus and bovine leukemia virus in water buffalo and cattle populations in Pakistan.
abstract: A survey of antibodies to bovine immunodeficiency virus (BIV) known as bovine lentivirus and bovine leukemia virus (BLV) was conducted with samples from water buffalo and cattle populations in Pakistan. A total of 370 water buffaloes and 76 cattle were tested, and 10.3% and 15.8%, respectively, were found positive for anti-BIV p26 antibodies determined by Western blotting, while 0.8% of water buffaloes and no cattle were positive for anti-BLV antibodies determined by immunodiffusion test. BIV-seropositive water buffaloes and cattle were found to have BIV proviral DNA in the peripheral blood mononuclear cells determined by nested polymerase chain reaction. This is the first report of BIV infections in water buffaloes.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Meas
authors.forename: J
authors.initials: J
authors.lastname: Seto
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: M
authors.initials: M
authors.lastname: Bakhsh
authors.forename: M
authors.initials: M
authors.lastname: Riaz
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Naeem
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: M
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D016583:Enzootic Bovine Leukosis / Q000453:epidemiology*; D016087:Immunodeficiency Virus, Bovine; D016180:Lentivirus Infections / Q000453:epidemiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral