title: Effects of neoplastic transformation and teniposide (VM26) on protein kinase C isoform expression in rodent fibroblasts.
abstract: This study examined changes in protein kinase C (PKC) isoforms in rodent fibroblasts (rat F111 and mouse NIH3T3), transformed by the polyoma virus middle T antigen (mT) and undergoing apoptosis in response to teniposide (VM26). The mT-transformed cells up-regulated PKC delta and down-regulated both PKC epsilon and PKC lambda expression, and were more sensitive to the drug than their non-transformed counterparts. The drug treatment further lowered the expression of PKC epsilon, triggered nuclear translocation of PKC delta and its site-specific proteolysis, consistent with the notion that changes in specific PKC isoforms play a role not only in the neoplastic transformation of fibroblasts, but also in their apoptotic response.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Canada.
authors.forename: Q Y
authors.initials: QY
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Carson
authors.forename: M
authors.initials: M
authors.lastname: Ribecco
authors.forename: L
authors.initials: L
authors.lastname: Testolin
authors.forename: L
authors.initials: L
authors.lastname: Raptis
authors.forename: P R
authors.initials: PR
authors.lastname: Walker
authors.forename: M
authors.initials: M
authors.lastname: Sikorska
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D015870:Gene Expression; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D011493:Protein Kinase C / Q000378:metabolism*; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D051381:Rats; D013713:Teniposide / Q000494:pharmacology*; D000099293:Protein Kinase C-lambda
chemical_list: D000970:Antineoplastic Agents; D007527:Isoenzymes; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D013713:Teniposide; D000099293:Protein Kinase C-lambda; C497259:Prkcd protein, mouse; C497260:Prkcd protein, rat; C497256:Prkce protein, mouse; C497257:Prkce protein, rat