X-ray analysis of a DNA dodecamer containing 2'-deoxy-N4-methoxycytidine.
The crystal structure of DNA dodecamer with the sequence of d(CGCAAATTXGCG), where X is 2'-deoxy-N4-methoxycytidine, has been determined by X-ray analysis. The dodecamers form a double helix with B-form conformation. The electron density indicates that the two modified cytosine bases respectively make a pair with the adenine bases on the opposite strand in a manner of Watson-Crick geometry and that the methoxy groups are in anti conformation to the N3 atom.
Hikima
T
T
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Hossain
M T
MT
Chatake
T
T
Ueno
Y
Y
Matsuda
A
A
Takénaka
A
A
D020029:Base Pairing; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D003841:Deoxycytidine / Q000031:analogs & derivatives*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
C409810:2'-deoxy-N(4)-methoxycytidine; D009838:Oligodeoxyribonucleotides; D003841:Deoxycytidine; D004247:DNA