title: In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin secretion.
abstract: OBJECTIVE
  To investigate the role of Transforming Growth Factor beta 1 on differentiation of human endometrial stromal cells.
  
  
  METHODS
  A prospective study.
  
  
  METHODS
  Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence and absence of TGF b 1. The influence of TGF beta 1 were measured by prolactin production expressed as nanograms of prolactin/mg of total DNA in cells measured by diphenylamine reaction.
  
  
  RESULTS
  The levels of prolactin in the culture medium with and without TGF beta 1 were 0.16 +/- 0.27 ng/microgram DNA/day and 0.24 +/- 0.41 ng/microgram DNA/day respectively. There was no significant difference between groups.
  
  
  CONCLUSIONS
  There is no direct effect of TGF beta 1 on differentiation of human endometrial stromal cells. However it may involve in more complex relationship in this process.
authors.affiliation: Dr. Zekai Tahir Burak Maternity Hospital, Ankara, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Celikkanat
authors.forename: B
authors.initials: B
authors.lastname: Atac
authors.forename: K
authors.initials: K
authors.lastname: Piskin
authors.forename: A
authors.initials: A
authors.lastname: Helvacioglu
authors.forename: O
authors.initials: O
authors.lastname: Gokmen
authors.forename: N
authors.initials: N
authors.lastname: Cakar
authors.forename: M S
authors.initials: MS
authors.lastname: Beksac
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans; D011388:Prolactin / Q000378:metabolism*; D011446:Prospective Studies; D017154:Stromal Cells; D016212:Transforming Growth Factor beta / Q000502:physiology*
chemical_list: D016212:Transforming Growth Factor beta; D011388:Prolactin