p38/SAPK2-dependent gene expression in Jurkat T cells.
The stress-activated protein kinase p38/SAPK2 is known to regulate the activity of transcription factors and to control expression of several genes at the transcriptional or post-transcriptional level. In order to identify genes whose expression is under the control of p38/SAPK2 activity, we have compared the mRNA levels of a pattern of 588 genes between human Jurkat T cells with anisomycin-activated p38/SAPK2 and cells in which p38/SAPK2 was inhibited by the compound SB203580. Genes strongly expressed at the transcript level as a result of p38/SAPK2 activation are the transcription factors c-jun, fos-related antigen 1 (fra-1), the growth-arrest and DNA-damage gene gadd153 and early-growth-related gene 1 (egr-1) as well as the c-srk kinase csk and the nucleotide exchange factor ras-GRF. mRNAs significantly down-regulated include the insulin receptor IR, the adapter grb2, the transcription factor c-myc and the defender against apoptotic death, dad-1. For six selected genes, p38/SAPK2-regulated expression was confirmed and further analysed by Northern blot experiments, demonstrating a complex regulation of these genes under stress conditions.
Rolli-Derkinderen
M
M
Innovationskolleg Zellspezialisierung der Martin-Luther-Universität Halle/Wittenberg, Halle, Germany.
Gaestel
M
M
D000841:Anisomycin / Q000494:pharmacology; D015152:Blotting, Northern; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D019169:Jurkat Cells; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases
D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D012333:RNA, Messenger; D000841:Anisomycin; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580