[Study of the photodynamic effect of new photosensitizers on the single neuron].
We studied reactions of isolated crayfish mechanoreceptor neurons to photodynamic effects of various photosensitizers: methylene blue, chlorins e6 and p6, sulfated allumophthalocyanin Photosens, Janus green B, protoporphyrin IX, and two derivatives of hematoporphyrin IX, Photoheme and Photosan-3. The neurons were irradiated by a helium-neon laser (632.8 nm, 0.3 Wt/cm3) after 30-min photosensitization. They proved to be very sensitive to the photodynamic effect: When the cells stained by photosensitizers at nanomolar concentrations were irradiated, their firing activity underwent irreversible changes and they died. The dynamics of the firing activity of the neurons depended on the photosensitizer type and concentration. Photosens, Photoheme and chlorin p6 proved to be the most efficient.
Uzdenskiĭ
A B
AB
Rostov State University, Rostov-on-Don, Russia. uzd@krinc.ru
Zhavoronkova
A A
AA
Mironov
A F
AF
Kuz'min
S G
SG
D000200:Action Potentials; D000818:Animals; D003400:Astacoidea; D016923:Cell Death; D066298:In Vitro Techniques; D007834:Lasers; D008465:Mechanoreceptors / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects*; D017319:Photosensitizing Agents / Q000494:pharmacology*
D017319:Photosensitizing Agents