New adsorbed coatings for capillary electrophoresis.
New acrylic polymers bearing oxirane groups were synthesized to be used in the production of coated capillaries. A fully automated coating procedure was devised based on the use of diluted water solutions of these polymers. The whole procedure required less than 30 min. The new polymers rapidly adsorbed from water onto the capillary wall, thus suppressing electroosmotic flow (EOF) to a negligible value. The adsorbed coatings were stable for hundreds of hours at high pH, temperature, and in the presence of 8 M urea. Efficient separations of acidic and basic proteins were achieved in the new phases.
Chiari
M
M
Institute of Biocatalysis and Molecular Recognition, CNR, Milano, Italy. chiari@ico.mi.cnr.it
Cretich
M
M
Damin
F
F
Ceriotti
L
L
Consonni
R
R
D000327:Adsorption; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004355:Drug Stability; D004563:Electrochemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009995:Osmosis; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D012996:Solutions; D013696:Temperature; D014867:Water
D011108:Polymers; D011506:Proteins; D012996:Solutions; D014867:Water