title: Characterization of a new TSPY gene family member in Yq (TSPYq1).
abstract: We investigated subinterval 6E on the human Y chromosome, a region frequently deleted in infertile males. YAC yOX17, mapped within subinterval 6E by STS-PCR, was analyzed for the presence of new genes. TSPYq1, a member of the TSPY multi-copy gene family, was isolated and characterized from a yOX17 cosmid subclone. PCR and FISH analysis performed on normal subjects and on patients with microdeletions of Yq suggested the presence of multiple copies of TSPY in Yq.
authors.affiliation: Istituto di Clinica Neurologica, Università di Milano, Ospedale Policlinico IRCCS, Chieti (Italy).
authors.forename: A
authors.initials: A
authors.lastname: Ratti
authors.forename: L
authors.initials: L
authors.lastname: Stuppia
authors.forename: V
authors.initials: V
authors.lastname: Gatta
authors.forename: I
authors.initials: I
authors.lastname: Fogh
authors.forename: G
authors.initials: G
authors.lastname: Calabrese
authors.forename: A
authors.initials: A
authors.lastname: Pizzuti
authors.forename: G
authors.initials: G
authors.lastname: Palka
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002872:Chromosome Deletion; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons / Q000235:genetics; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009687:Nuclear Proteins; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D051876:Sex-Determining Region Y Protein; D013091:Spermatogenesis / Q000235:genetics; D014157:Transcription Factors; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497749:TSPY1 protein, human; D014157:Transcription Factors