title: Allelic expression of the putative tumor suppressor gene p73 in human fetal tissues and tumor specimens.
abstract: p73, a proposed tumor suppressor, shares significant amino acid sequence homology with p53. However, p73 is rarely mutated in tumors but it has been suggested that p73 is monoallelically expressed in some tissues. This latter feature would predispose p73 to gene inactivation because a single genetic 'hit' or the loss of the expressed parental allele would leave the cell without p73 activity. We examined the allelic expression of p73 in normal fetal tissues and in ovarian cancer and Wilms' tumor. We found that p73 was biallelically expressed in all fetal tissues, except in brain, where differential expression of the two parental alleles was observed. Biallelic expression of p73 was also observed in paired samples of ovary cancer and Wilms' tumor. Loss of heterozygosity of p73 occurred at relatively low rates in tumors: one of 11 informative samples (9.1%) of ovarian cancer and two of 19 (10.1%) Wilms' tumors. These data demonstrate that p73 is biallelically expressed in most tissues, thus excluding genomic imprinting as a molecular mechanism to predispose to allelic inactivation of p73 in human tumors.
authors.affiliation: GRECC and Medical Service, VA Palo Alto Health Care System, and Division of Endocrinology, Department of Medicine, Stanford University, Palo Alto, CA, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Hu
authors.forename: G A
authors.initials: GA
authors.lastname: Ulaner
authors.forename: H
authors.initials: H
authors.lastname: Oruganti
authors.forename: R D
authors.initials: RD
authors.lastname: Ivaturi
authors.forename: K A
authors.initials: KA
authors.lastname: Balagura
authors.forename: J
authors.initials: J
authors.lastname: Pham
authors.forename: T H
authors.initials: TH
authors.lastname: Vu
authors.forename: A R
authors.initials: AR
authors.lastname: Hoffman
mesh_terms: D000483:Alleles; D001921:Brain / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D005333:Fetus; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics; D014407:Tumor Cells, Cultured; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D009396:Wilms Tumor / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins