NK-3 receptors are expressed on mouse striatal gamma-aminobutyric acid-ergic interneurones and evoke [(3)H] gamma-aminobutyric acid release.
In the present study the ability of tachykinin agonists and antagonists to modulate gamma-aminobutyric acid (GABA) release has been correlated with tachykinin receptor expression in the mouse striatum. Significant GABA release was observed when striatal slices were challenged with the NK-3 receptor agonist senktide, the selectivity of which was confirmed using the NK-3 receptor antagonist SR142801. In situ hybridisation revealed co-expression of NK-3 receptors with nitric oxide synthase (NOS)/preprosomatostatin containing GABAergic interneurones. These findings suggest that tachykinins modulate GABA release within the striatum via interaction with NK-3 receptors on somatostatin/NOS interneurones.
Preston
Z
Z
Parke-Davis Neuroscience Research Centre, Cambridge University Forvie Site, Robinson Way, Cambridge, UK.
Richardson
P J
PJ
Pinnock
R D
RD
Lee
K
K
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D017403:In Situ Hybridization; D007395:Interneurons / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000032:analysis; D010446:Peptide Fragments / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D011498:Protein Precursors / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D018042:Receptors, Neurokinin-3 / Q000235:genetics*; D013004:Somatostatin / Q000032:analysis / Q000378:metabolism; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology; D015320:Tachykinins / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D010446:Peptide Fragments; D010880:Piperidines; D011498:Protein Precursors; D012333:RNA, Messenger; D018042:Receptors, Neurokinin-3; D015320:Tachykinins; C054724:senktide; D009569:Nitric Oxide; D013373:Substance P; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid; D019001:Nitric Oxide Synthase; C091554:SR 142801; D000109:Acetylcholine