Identification of smoked Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) using PCR-restriction fragment length polymorphism of the p53 gene.
Raw and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the p53 gene. DNA from S. salar and 0. mykiss was amplified by using primers flanking exons 5 to 6 of the p53 nuclear gene. PCR products of different length were obtained for each species (532 and 518 base pairs, respectively). Sequences of PCR products obtained from S. salar and O. mykiss were compared in the search for polymorphic restriction sites. The restriction fragments obtained with Eco RV, Hinf I, and Taq I endonucleases showed interspecific polymorphism, making it a useful method for identification of Atlantic salmon and rainbow trout.
Carrera
E
E
Universidad Complutense, Facultad de Veterinaria, Departamento de Nutrición y Bromatología III, Madrid, Spain.
García
T
T
Céspedes
A
A
González
I
I
Fernández
A
A
Asensio
L M
LM
Hernández
P E
PE
Martín
R
R
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific; D005504:Food Analysis; D005511:Food Handling; D016158:Genes, p53; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020317:Salmo salar / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C027440:GATATC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases