Synthesis and properties of oligonucleotides containing 2'-O-methyl-2-thiouridine.
New methods to synthesize 2'-O-methyl-2-thiouridine and its phosphoramidite building block for incorporation into oligonucleotides were developed. Oligonucleotides containing 2'-O-methyl-2-thiouridine were expected to be favorable as antisense agents in several respects, i.e., nuclease resistance, stable RNA duplex formation, and exact base recognition. Therefore, to make them clear, we synthesized oligonucleotides having 2'-O-methyl-2-thiouridine and analyzed their properties in detail.
Shohda
K
K
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Okamoto
I
I
Wada
T
T
Sekine
M
M
D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis
C409967:2'-O-methyl-2-thiouridine; D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D013891:Thiouridine; D012313:RNA