Rapid detection of the gene of Legionella pneumophila using the fluorescence polarization with the asymmetric PCR.
We attempted the rapid detection method of Legionella pneumophila by the asymmetric PCR and the fluorescence polarization. Eleven extracted DNAs from L. pneumophila serogroup 1 to approximately 6, L. bozemanii, L. dumoffii, L. gormanii, L. micdadei, and Pseudomonas aeruginosa were amplified by asymmetric PCR, and the polarization of those products were measured. Only the polarization of L. pneumophila serogroup 1 to approximately 6 rose within a few minutes after the beginning of measurement. The sensitivity to L. pneumophila using this method was 10(3) cells.
Fujii
T
T
Towa Kagaku Co. Ltd., Hiroshima, Japan.
Ohta
M
M
Kono
M
M
Hoshina
S
S
Fukuhara
K
K
Tsuruoka
M
M
D000818:Animals; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D005454:Fluorescence Polarization / Q000379:methods; D007875:Legionella / Q000145:classification / Q000235:genetics; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012680:Sensitivity and Specificity; D012703:Serotyping
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal