Purification of the newly found selenium-containing proteins in the arterial wall and brain of the rat.
Previously several selenium-containing proteins with different subunit molecular masses (M(r)) were detected in the arterial wall and brain of rats. In continuation of this work, after labeling of rats in vivo with [(75)Se]selenite, the new selenium-containing proteins of interest were purified on a Sephadex G-200 column followed by preparative isoelectric focusing. Nuclear analytical methods (gamma-counter and gamma-detector) were applied in the detection and identification of the (75)Se-labeled proteins. The two (75)Se-containing proteins from the arterial wall migrated as 15.0- and 67.0-kDa species on SDS-PAGE gels with pI values of 4.5 and 5.1, respectively. The three (75)Se-containing proteins from brain purified to homogeneity had M(r) values of 18.0, 30.0, and 42.9 kDa and pI values of 6.3, 6.5, and 6.0, respectively. Of these proteins, the 67.0-, 42.9-, and 30.0-kDa species may be yet not characterized selenoproteins with important biological functions.
Qu
X
X
Department of Chemistry, Huazhong University of Science and Technology, Wuhan, 430074, People's Republic of China. quxh@nic.bmi.ac.cn
Huang
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K
Wu
Z
Z
Zhong
S
S
Chen
Z
Z
Xu
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H
D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000378:metabolism; D051140:Selenoproteins; D018038:Sodium Selenite / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D011506:Proteins; C495241:Selenof protein, rat; D051140:Selenoproteins; D013462:Sulfur Radioisotopes; D012643:Selenium; D018038:Sodium Selenite