title: Metabolism of the dorsal cochlear nucleus in rat brain slices.
abstract: In vitro brain slices of the cochlear nucleus have been used for electrophysiological and pharmacological studies. More information is needed about the extent to which the slice resembles in vivo tissue, since this affects the interpretation of results obtained from slices. In this study, some chemical parameters of the dorsal cochlear nucleus (DCN) in rat brain slices were measured and compared to the in vivo state. The activities of malate dehydrogenase and lactate dehydrogenase were reduced in some DCN layers of incubated slices compared to in vivo brain tissue. The activities of choline acetyltransferase and acetylcholinesterase were increased or unchanged in DCN layers of slices. Adenosine triphosphate (ATP) concentrations for in vivo rat DCN were similar to those of cerebellar cortex. Compared with in vivo values, ATP concentrations were decreased in the DCN of brain slices, especially in the deep layer. Vibratome-cut slices had lower ATP levels than chopper-cut slices. Compared with the in vivo data, there were large losses of aspartate, glutamate, glutamine, gamma-aminobutyrate and taurine from incubated slices. These amino acid changes within the slices correlated with the patterns of release from the slices.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Medical College of Ohio, 3065 Arlington Avenue, Toledo, OH 43614, USA.
authors.forename: L
authors.initials: L
authors.lastname: Zheng
authors.forename: D A
authors.initials: DA
authors.lastname: Godfrey
authors.forename: H J
authors.initials: HJ
authors.lastname: Waller
authors.forename: T G
authors.initials: TG
authors.lastname: Godfrey
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Kong
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D017626:Cochlear Nucleus / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000596:Amino Acids; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase