Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice.
To define a gene expression mechanism, it is often advantageous to use a reporter gene and transgenic mouse. The lacZ reporter gene is particularly useful for studies of the cis-regulatory element for tissue-specific expression in transgenic mice because of the ease of the enzyme assay and visualization on sections. In this report, we describe our method for examining the cis-regulatory element in transgenic mice, including choice of the lacZ gene, generation of transgenic mice, and analysis of beta-galactosidase activity.
Takahashi
E
E
Tsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Tsukuba, Japan. e2-takahashi@hhc.eisai.co.jp
Miyamoto
N
N
Kajiwara
N
N
Furuya
K
K
Yanai-Taniguchi
K
K
Sugiyama
F
F
Yagami
K
K
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D020865:Calcium Channels, P-Type / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D013194:Staining and Labeling; D014018:Tissue Distribution; D001616:beta-Galactosidase / Q000378:metabolism
D020865:Calcium Channels, P-Type; D001616:beta-Galactosidase