IDPN-induced monoamine and hydroxyl radical changes in the rat brain.
beta-beta'-iminodipropionitrile (IDPN)-induced monoamine and hydroxyl radical changes in the rat brains were studied. IDPN caused decreases in 5-HT and 5-HIAA levels in all brain regions, strongly indicating that IDPN's neurotoxicity primarily affects 5-HT containing neurons. Dopamine and its metabolites' levels decreased in the some regions, most likely due to depression of dopamine metabolic turnover. Our results more clearly demonstrate IDPN-induced monoamine alterations in the rat brain more than previous reports. To clarify one of the pathogenesis of IDPN-induced neurological disorders, we measured hydroxyl radical levels. 2,3-DHBA increased at 1st day, and decreased in some regions at 7th days after discontinuing IDPN. We conclude, hydroxyl radical formation causes neuronal damage, and monoamine changes contribute to IDPN-induced neurological disorder.
Wakata
N
N
Fourth Department of Internal Medicine, Toho University, Tokyo, Japan. waka@oha.toho-u.ac.jp
Araki
Y
Y
Sugimoto
H
H
Iguchi
H
H
Kinoshita
M
M
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D006719:Homovanillic Acid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism*; D008297:Male; D009422:Nervous System Diseases / Q000139:chemically induced / Q000378:metabolism*; D009570:Nitriles / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism
D015306:Biogenic Monoamines; D009570:Nitriles; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D017665:Hydroxyl Radical; C008110:3,3'-iminodipropionitrile; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D006719:Homovanillic Acid