Cholinesterases in dexrazoxane-treated daunorubicin cardiomyopathy in rabbits.
Changes in cholinesterases activities in daunorubicin cardiomyopathy and in dexrazoxane (DRZX)-treated daunorubicin cardiomyopathy were investigated in rabbits. Acetyl- and butyrylcholinesterase (AChE and BuChE) were determined using Ellman's method. In the serum, a significant decrease of BuChE was observed in the daunorubicin group (9.05 at the beginning and 7.15 microcat/l at the end of the experiment). After DRZX, no significant changes were found and a significant increase in BuChE was observed in the control group (10.26-12.38 microcat/l). AChE activity in the left and right cardiac ventricles was not significantly different between the groups while in the septum there was a significantly lower AChE activity found in the daunorubicin group only. BuChE activity was significantly decreased in the left (15.64 ncat/g) and right (19.27 ncat/g) heart ventricles, in the septum and in the liver in the daunorubicin group. A significant decrease in serum total protein and albumin was demonstrated only in the daunorubicin group. Our results support the hypothesis about the influence of daunorubicin on protein (and enzyme) synthesis in the liver and heart. A protective effect of DRZX on cholinesterases activity was observed. The changes in cholinesterase activities may thus reflect their possible role in cardiomyopathy.
Gersl
V
V
Department of Pharmacology, Faculty of Medicine in Hradec Králové, Charles University in Prague, Czech Republic. gersl@lfhk.cuni.cz
Bajgar
J
J
Hrdina
R
R
Mazurová
Y
Y
Machácková
J
J
Cerman
J
J
Suba
P
P
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D002091:Butyrylcholinesterase / Q000378:metabolism*; D009202:Cardiomyopathies / Q000139:chemically induced / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D002317:Cardiovascular Agents / Q000494:pharmacology*; D003630:Daunorubicin / Q000633:toxicity*; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D011817:Rabbits; D011929:Razoxane / Q000494:pharmacology*; D016277:Ventricular Function, Left / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D002317:Cardiovascular Agents; D011929:Razoxane; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D003630:Daunorubicin