title: Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2).
abstract: We report the identification and characterization of a novel CC chemokine designated CCL28 and its receptor CCR10, known previously as orphan G-protein-coupled receptor GPR2. Human and mouse CCL28 share 83% identity at the amino acid and 76% at the nucleic acid levels. We also identified the mouse homologues of CCL28 and of CCR10, which map to mouse chromosomes 13 and 11, respectively. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. Both human and mouse CCL28 induce calcium mobilization in human and mouse CCR10-expressing transfectants. CCL28 desensitized the calcium mobilization induced in CCR10 transfectants by CCL27, indicating that these chemokines share this new chemokine receptor. In vitro, recombinant human CCL28 displays chemotactic activity for resting CD4 or CD8 T cells.
authors.affiliation: Department of Immunobiology, DNAX Research Institute, Palo Alto, California 94304, USA.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Soto
authors.forename: E R
authors.initials: ER
authors.lastname: Oldham
authors.forename: M E
authors.initials: ME
authors.lastname: Buchanan
authors.forename: B
authors.initials: B
authors.lastname: Homey
authors.forename: D
authors.initials: D
authors.lastname: Catron
authors.forename: N
authors.initials: N
authors.lastname: Jenkins
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: D J
authors.initials: DJ
authors.lastname: Gilbert
authors.forename: N
authors.initials: N
authors.lastname: Nguyen
authors.forename: J
authors.initials: J
authors.lastname: Abrams
authors.forename: D
authors.initials: D
authors.lastname: Kershenovich
authors.forename: K
authors.initials: K
authors.lastname: Smith
authors.forename: T
authors.initials: T
authors.lastname: McClanahan
authors.forename: A P
authors.initials: AP
authors.lastname: Vicari
authors.forename: A
authors.initials: A
authors.lastname: Zlotnik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018925:Chemokines / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D019742:Chemokines, CC / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D006801:Humans; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D054447:Receptors, CCR10; D019707:Receptors, Chemokine / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: C414047:CCL28 protein, human; C515706:CCR10 protein, human; C515707:Ccr10 protein, mouse; D018925:Chemokines; D019742:Chemokines, CC; D008024:Ligands; D054447:Receptors, CCR10; D019707:Receptors, Chemokine