title: Binding sites for melanin-concentrating hormone in the human brain.
abstract: Binding sites for melanin-concentrating hormone (MCH) in human brain were investigated and characterized by radioligand binding. Specific binding sites for MCH were present in every region of human brain (cerebral cortex, cerebellum, thalamus, hypothalamus, pons, and medulla oblongata) obtained at autopsy. alpha-Melanocyte stimulating hormone or ACTH was a poor inhibitor of (125)I-MCH binding (IC(50) 1 microM) compared with MCH (IC(50) = 0.3 +/- 0.07 nM, mean +/- SEM, n = 3). Scatchard plots of (125)I-MCH binding in human brain (thalamus) gave a dissociation constant of 0.2 +/- 0.06 nM and maximal binding of 5.8 +/- 0.3 fmol/mg protein (n = 3). These findings suggest that specific MCH binding sites that differ from the melanocortin receptors exist in human brain.
authors.affiliation: Department of Internal Medicine, National Iwate Hospital, Ichinoseki, Iwate, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sone
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: O
authors.initials: O
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Totsune
authors.forename: Z
authors.initials: Z
authors.lastname: Arihara
authors.forename: F
authors.initials: F
authors.lastname: Satoh
authors.forename: H
authors.initials: H
authors.lastname: Sasano
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Mouri
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000328:Adult; D000368:Aged; D001665:Binding Sites; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007028:Hypothalamic Hormones / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D008543:Melanins / Q000378:metabolism*; D008875:Middle Aged; D009479:Neuropeptides / Q000494:pharmacology; D010907:Pituitary Hormones / Q000378:metabolism*; D011485:Protein Binding; D011869:Radioligand Assay; D018043:Receptors, Corticotropin / Q000378:metabolism; D044101:Receptors, Melanocortin; D000521:alpha-MSH / Q000494:pharmacology
chemical_list: D007028:Hypothalamic Hormones; D007457:Iodine Radioisotopes; D008543:Melanins; D009479:Neuropeptides; D010907:Pituitary Hormones; D018043:Receptors, Corticotropin; D044101:Receptors, Melanocortin; D000521:alpha-MSH; C019463:melanin-concentrating hormone; D000324:Adrenocorticotropic Hormone