[The effect of carbon sources and mononucleotides on the production of extracellular alkaline phosphatase by Bacillus intermedius].
The biosynthesis of extracellular alkaline phosphatase in the streptomycin-resistant strains Bacillus intermedius S3-19 and S7 in the presence in the medium of 5'-nucleoside monophosphates and different sources of carbon--glucose, sodium pyruvate, sodium lactate, or glycerol--was studied. It was established that, in the presence of mononucleotides, the content of extracellular alkaline phosphatase in both strains increased; the maximal effect was caused by 5'-AMP at a concentration of 20 micrograms/ml. In medium with a low orthophosphate content, where active biosynthesis of alkaline phosphatase occurred, 1% glucose and 0.5% pyruvate stimulated this process 2.5-4 times, and 2% sodium lactate and sodium pyruvate, on the contrary, inhibited it by 20-40%. Analysis of the dynamics of growth and accumulation of extracellular phosphatase in the presence of different sources of carbon in the medium gives evidence of an interrelationship between the biosynthesis of alkaline phosphatase and carbon metabolism in Bacillus intermedius.
Sharipova
M R
MR
Kazan State University, Tatarstan, Russia. margarita.sharipova@ksu.ru
Balaban
N P
NP
Nekhotiaeva
N V
NV
Leshchinskaia
I B
IB
D000249:Adenosine Monophosphate / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D001407:Bacillus / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*
D000249:Adenosine Monophosphate; D002244:Carbon; D019289:Pyruvic Acid; D000469:Alkaline Phosphatase