Reversible photoligation of DNA via 5-vinyldeoxyuridine.
We report a novel non-enzymatic template-directed reversible photoligation of oligodeoxyribonucleotides (ODNs). In this photoligation system, a modified ODN containing 5-vinyldeoxyuridine at 5'-end reacts by photoirradiation at 366 nm with cytosine and thymine at the 3'-end of another ODN in the presence of a complementary template to yield a ligated ODN quantitatively. The ligated ODN can be split site-selectively to regenerate the parent ODN by photoirradiation at 302 nm.
Fujimoto
K
K
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Japan.
Matsuda
S
S
Saito
I
I
D001483:Base Sequence; D003596:Cytosine; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D003857:Deoxyuridine / Q000031:analogs & derivatives / Q000138:chemical synthesis; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000528:radiation effects; D013698:Templates, Genetic; D013941:Thymine
D009838:Oligodeoxyribonucleotides; C011381:5-vinyl-2'-deoxyuridine; D003596:Cytosine; D004247:DNA; D013941:Thymine; D003857:Deoxyuridine