Tetracycline-regulated gene expression switch in Xenopus laevis.
Xenopus is a well-characterized model system for the investigation of biological processes at the molecular, cellular, and developmental level. The successful application of a rapid and reliable method for transgenic approaches in Xenopus has led to renewed interest in this system. We have explored the applicability of tetracycline-regulated gene expression, first described by Gossen and Bujard in 1992, to the Xenopus system. By optimizing conditions, tetracycline repressor induced expression of a luciferase reporter gene was readily and reproducibly achieved in both the Xenopus oocyte and developing embryo. This high level of expression was effectively abrogated by addition of low levels of tetracycline. The significance of this newly defined system for studies of chromatin dynamics and developmental processes is discussed.
Ridgway
P
P
Section de Recherche UMR218 du CNRS, Institut Curie, 26 rue d'Ulm, Paris Cedex 05, 75231, France.
Quivy
J P
JP
Almouzni
G
G
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins; D002843:Chromatin / Q000378:metabolism; D004625:Embryo, Nonmammalian; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics; D066298:In Vitro Techniques; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D009865:Oocytes / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics; D013752:Tetracycline / Q000494:pharmacology*; D014981:Xenopus
D001426:Bacterial Proteins; D002352:Carrier Proteins; D002843:Chromatin; D017405:Herpes Simplex Virus Protein Vmw65; D012097:Repressor Proteins; C083139:Tet O resistance protein, Bacteria; C034811:tetracycline resistance-encoding transposon repressor protein; D008156:Luciferases; D013752:Tetracycline