Establishment of a visible peritoneal micrometastatic model from a gastric adenocarcinoma cell line by green fluorescent protein.
To establish a visible peritoneal micrometastatic model, an enhanced green fluorescent protein (EGFP) expressing plasmid vector was transfected into the gastric cancer cell line, MKN-45. Highly expressing EGFP cells were injected into the peritoneal cavity of nude mice. Then, the peritoneum and abdominal organs were harvested and observed on days 1, 4 and 7. Fluorescence stereomicroscopy identified peritoneal micrometastases that had been undetected by stereomicroscopy. Micrometastases as small as a single cell are detectable in this model. This peritoneal micrometastatic model should be a useful tool for research on metastasis of gastric cancer.
Kaneko
K
K
Department of Surgery and Clinical Oncology, Osaka University Graduate School of Medicine, E-2, Suita, Osaka 565-0871, Japan.
Yano
M
M
Tsujinaka
T
T
Morita
S
S
Taniguchi
M
M
Fujiwara
Y
Y
Doki
Y
Y
Inoue
M
M
Shiozaki
H
H
Monden
M
M
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D005260:Female; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins; D006801:Humans; D007202:Indicators and Reagents; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000556:secondary*; D013274:Stomach Neoplasms / Q000473:pathology*; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins