Free DNA induces modification on the protein synthesis profile of human peripheral blood mononuclear cells of healthy donors.
Understanding how free DNA might act as a signal between cells is important for knowing how DNA orchestrates immune responses and for optimizing the therapeutic of cancer, infection and immunologic diseases. This communication demonstrates that DNAs from different origins (bacteria, T. cruzi, HeLa cells) and synthetic oligonucleotide containing an unmethylated CpG motif are capable of inducing alterations in the protein profile of normal human leukocytes. As far as we know there have been no similar studies regarding the comparative effects of different free DNAs on early protein synthesis of human peripheral blood mononuclear cells.
Martins
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GA
Laboratorio de Controle da Expressao Genica, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Centro de Ciencias da Saude, Ilha do Fundao, Rio de Janeiro, R.J. 21949-900, Brazil.
Gattas
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CR
da Costa Carvalho
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MG
D018899:CpG Islands; D004247:DNA / Q000378:metabolism* / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000494:pharmacology; D016054:DNA, Protozoan / Q000494:pharmacology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D009841:Oligonucleotides / Q000494:pharmacology; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects
D004269:DNA, Bacterial; D016054:DNA, Protozoan; D009841:Oligonucleotides; D011506:Proteins; D004247:DNA