title: Studies on the primary structure of short polysaccharides using SEC MALDI mass spectroscopy.
abstract: The introduction of size-exclusion chromatography (SEC) analysis of polysaccharides prior to MALDI mass spectroscopy accounts for the determination of the molecular mass of the repeating unit when neutral homopolymers are investigated. In the case of natural polysaccharides characterised by more complicated structural features (presence of non-carbohydrate substituents, charged groups, etc.), this mass value usually is in agreement with more than one sugar composition. Therefore, it is not sufficient to give the correct monosaccharidic composition of the polysaccharide investigated. To solve this problem, MALDI spectra were recorded on the permethylated sample and post-source decay experiments were performed on precursor ions. In this way, the composition (in terms of Hex, HexNAc, etc.), size and sequence of the repeating unit were determined.
authors.affiliation: CNR Istituto per la Chimica e la Tecnologia dei Materiali Polimerici, Catania, Italy. dgarozzo@dipchi.unict.it
authors.forename: D
authors.initials: D
authors.lastname: Garozzo
authors.forename: E
authors.initials: E
authors.lastname: Spina
authors.forename: R
authors.initials: R
authors.lastname: Cozzolino
authors.forename: P
authors.initials: P
authors.lastname: Cescutti
authors.forename: W F
authors.initials: WF
authors.lastname: Fett
mesh_terms: D003911:Dextrans / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D009005:Monosaccharides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D003911:Dextrans; D005936:Glucans; D009005:Monosaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C064841:exopolysaccharide, Pseudomonas; C009109:pullulan