title: Heterogeneity of autoantibodies against cardiolipin and oxidatively modified LDLs revealed by human monoclonal antibodies.
abstract: Human monoclonal antibodies against cardiolipin, native and oxidatively modified LDL and against apoB100 were obtained by Epstein-Barr virus transformation of peripheral blood mononuclear cells followed by cloning. The antibodies were used to analyse the cross-reactivity between monoclonals with different specificities and to study the repertoire of antibodies present in sera from patients. Monoclonal antibodies selected for binding to cardiolipin were the least cross-reactive, whereas antibodies against different modifications of LDL frequently also reacted with other modifications. Inhibition studies showed that serum antibodies against cardiolipin and against oxidatively modified LDL were to a different extent inhibited by the different monoclonal antibodies and thus markedly heterogeneous.
authors.affiliation: Immunological Research Unit, Center for Molecular Medicine and Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
authors.forename: A K
authors.initials: AK
authors.lastname: Lefvert
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001161:Arteriosclerosis / Q000175:diagnosis* / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001402:B-Lymphocytes / Q000276:immunology*; D002308:Cardiolipins / Q000276:immunology*; D002460:Cell Line; D008078:Cholesterol, LDL / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007457:Iodine Radioisotopes / Q000378:metabolism; D015227:Lipid Peroxidation
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D002308:Cardiolipins; D008078:Cholesterol, LDL; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes