title: Structure of the O-specific polysaccharide of Proteus mirabilis O11, another Proteus O-antigen containing an amide of D-galacturonic acid with L-threonine.
abstract: The O-specific polysaccharide of Proteus mirabilis O11 was studied by sugar analysis, Smith degradation, 1H and 13C NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, and 1H-detected 1H, 13C HMQC experiments. The following structure of a pentasaccharide repeating unit of the polysaccharide was established: [formual: see text] where D-GalA6LThr is N-(D-galacturonoyl)-L-threonine. ELISA with anti-P. mirabilis O11 serum showed that D-GalA6LThr is of minor importance for manifesting the O11 immunospecificity.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: N P
authors.initials: NP
authors.lastname: Arbatsky
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: E
authors.initials: E
authors.lastname: Literacka
authors.forename: G
authors.initials: G
authors.lastname: Widmalm
authors.forename: W
authors.initials: W
authors.lastname: Kaca
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000577:Amides / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006603:Hexuronic Acids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D013912:Threonine / Q000737:chemistry*
chemical_list: D000577:Amides; D006603:Hexuronic Acids; D019081:O Antigens; C410150:O-specific polysaccharide, Proteus mirabilis O11; D011134:Polysaccharides; D013912:Threonine; C007819:galacturonic acid