Mitomycin C-induced apoptosis in cultured human Tenon's capsule fibroblasts.
To investigate the mitomycin C-induced apoptotic cell death of fibroblasts, the primarily cultured human Tenon's capsule fibroblasts were exposed to a clinically used dosage of 0.4 mg/ml of mitomycin C for 5 minutes. TUNEL (TdT-mediated dUTP-biotin nick end labeling) assay and electron microscopic studies were performed to determine the extent of mitomycin C-induced apoptosis. A flow cytometric study was performed to quantify the apoptotic cell population over time. The TUNEL stains were positive and electron microscopy showed features of apoptotic cell death in some fibroblasts 3 and 5 days after treatment. Flow cytometric analysis using Annexin V-propidium iodide double staining detected apoptotic cells 3 days after treatment. These apoptotic cell populations increased at 4 days and were sustained for one week. This study revealed that the clinical effects of mitomycin C on fibroblasts may be mediated not only by antiproliferative but also apoptotic cell death to some degree. Therefore, the apoptotic cell death of fibroblasts induced by mitomycin C should be considered to properly understand the mechanism of wound healing after trabeculectomy with adjunctive mitomycin C.
Kim
J W
JW
Department of Ophthalmology, School of Medicine, Catholic University of Taegu-Hyosung, Korea.
Kim
S K
SK
Song
I H
IH
Kim
I T
IT
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003238:Connective Tissue / Q000187:drug effects* / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D016685:Mitomycin / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin