The stem of sinomenium acutum inhibits mast cell-mediated anaphylactic reactions and tumor necrosis factor-alpha production from rat peritoneal mast cells.
The aqueous extract of Sinomenium acutum stem (SSAE) (0.1-1000 mg/kg) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. In particular, SSAE reduced compound 48/80-induced anaphylactic reaction with 50% at the dose of 1000 mg/kg. SSAE (100-1000 mg/kg) also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with SSAE at a concentration ranging from 0.1 to 1000 mg/kg, the plasma histamine levels were reduced in a dose-dependent manner. SSAE (1-1000 microg/ml) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMCs) activated by compound 48/80 or anti-DNP IgE. In addition, SSAE (0.1 microg/ml) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha (TNF-alpha) production. These results indicate that SSAE inhibits mast cell-mediated anaphylactic reactions and TNF-alpha production from mast cells.
Kim
H M
HM
Department of Oriental Pharmacy, College of Pharmacy and Center of Oriental Medicinal Science, Wonkwang University, Iksan, Chonbuk, South Korea. hmkim@wonnms.wonkwang.ac.kr
Moon
P D
PD
Chae
H J
HJ
Kim
H R
HR
Chung
J G
JG
Kim
J J
JJ
Lee
E J
EJ
D000707:Anaphylaxis / Q000517:prevention & control*; D000818:Animals; D006636:Histamine Release / Q000187:drug effects; D007073:Immunoglobulin E / Q000276:immunology; D007723:Korea; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010537:Peritoneum / Q000166:cytology; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine