Eicosanoid production and lymphatic responsiveness in human cigarette smokers compared with non-smokers.
Leg lymphatic segments were isolated from 10 patients (4 cigarette smokers and 6 non-smokers) undergoing conventional lymphography. Prostaglandin (PG) levels and PG synthesis in the lymphatics and in a variety of body fluids and the effects of eicosanoids on lymphatic contractility were determined. Leg lymphatics from 4 smokers generated less PGI2 and contained more 8-epi-PGF2 alpha when compared with leg lymphatics in 6 non-smokers. Similarly, levels of 8-epi-PGF2 alpha in smokers compared with non-smokers were higher in plasma (28.6 cf 19.7 pg/ml), leg lymph (146.7 cf 65.3 pg/ml), serum (299.0 cf 204.1 pg/ml), and urine (473.4 cf 241.0 pg/mg creatinine). Lymphatics from smokers also showed a higher contractile response, less 14C-arachidonic acid conversion to PGI2 and less PGI2-formation with various stimuli compared with non-smokers. Together these findings suggest that smoking induces oxidation injury, promotes altered (iso-)eicosanoid production and impacts on the function and dysfunction of peripheral lymphatics under normal circumstances and in a variety of clinical disorders.
Sinzinger
H
H
Wilhelm Auerswald Atherosclerosis Research Group Vienna, Austria. Helmut.Sinzinger@akh-wien.ac.at
Kaliman
J
J
Oguogho
A
A
D015777:Eicosanoids / Q000378:metabolism*; D006801:Humans; D008196:Lymph / Q000502:physiology*; D008208:Lymphatic System / Q000503:physiopathology; D018384:Oxidative Stress / Q000502:physiology; D011453:Prostaglandins; D012016:Reference Values; D012907:Smoking / Q000503:physiopathology*
D015777:Eicosanoids; D011453:Prostaglandins