title: DNA damage, cell kinetics and ODC activities studied in CBA mice exposed to electromagnetic fields generated by transmission lines.
abstract: CBA mice were exposed outdoors to 50 Hz electromagnetic fields (EMF), with a flux density of about 8 microT rms (root mean square), generated by a 220 kV transmission line. Assays were performed in order to investigate, the possible genotoxic effects after 11, 20 and 32 days of exposure, as well as the effects on body weight, leukocytes, erythrocytes, and the level of ornithine decarboxylase (ODC) activity in spleen and testis. DNA migration was studied on brain cells by single cell electrophoresis (comet assay). After 32 days of exposure a highly significant change of the tail/head ratio of the comets was observed (p < 0.001), showing DNA-damage. Further, a decreased number of mononuclear leukocytes (0.02 < p < 0.05) was observed in mice EMF-exposed for 20 days. In summary, our data indicate that transmission lines of this type may induce genotoxic effects in mice, seen as changes in the DNA migration. These results might have an important implication for health effects.
authors.affiliation: Department of Radioecology, Swedish University of Agricultural Sciences, Uppsala, Sweden. svedenstal@delta.telenordia.se
authors.forename: B M
authors.initials: BM
authors.lastname: Svedenstål
authors.forename: K J
authors.initials: KJ
authors.lastname: Johanson
authors.forename: M O
authors.initials: MO
authors.lastname: Mattsson
authors.forename: L E
authors.initials: LE
authors.lastname: Paulsson
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000528:radiation effects; D002453:Cell Cycle / Q000276:immunology / Q000528:radiation effects; D004249:DNA Damage; D011211:Electric Power Supplies; D004574:Electromagnetic Fields / Q000009:adverse effects*; D007962:Leukocytes / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology / Q000528:radiation effects; D013737:Testis / Q000201:enzymology / Q000528:radiation effects; D015430:Weight Gain / Q000528:radiation effects
chemical_list: D009955:Ornithine Decarboxylase