Comparative study of platelet histamine and serotonin with their corresponding plasma oxidases in asthmatics with normals.
OBJECTIVE
Asthma is well controllable but non-curable disease. Exact pathophysiology involved is unresolved till today. Role of allergic hypersensitivity reaction in asthmatic on-set is well established. Present work is an effort to elucidate some basic points of unresolved pathophysiology of asthma taking platelets as marker.


METHODS
A group of 52 normal human subjects in the age group of 20-60 years were studied for platelet histamine and serotonin levels and also for their plasma metabolising enzymes diamine oxidase (DAO) and monoamine oxidase (MAO). The data was collected for 79 asthmatic patients at different stages of asthma and accordingly were studied as four different groups of seventy nine asthmatics those were on regular treatment and were comfortable with drugs and were free from symptomatic attack formed gr. I; these (79) patients were followed-up during their symptomatic phase (gr. II) and same (79) patients immediately after their recovery from symptomatic stage studied as gr. III members. All the 79 asthmatic patients fall in gr. I, II and III in a serial manner i.e. all (n = 79) in each group. A separate group of thirty seven patients with known history of asthma but were symptom free and also off drugs for last 2-4 years formed gr. IV.


RESULTS
Results showed mean platelet count in asthmatics at all four stages were in the normal range but were slightly low in comparison with normals. Both the enzymatic levels (DAO and MAO) in gr. I, II and III were significantly higher than normals but were same in the case of gr. IV patients. Low levels of platelet biogenic amines were observed in asthmatics (gr. I to gr. IV) than normals.


CONCLUSIONS
Thus, study parameters showed significant difference in asthmatics and normals. Findings of the study have been utilized to understand unanswered hypersensitivity shown by the asthmatics over normal individuals (non-asthmatics).
Matkar
N M
NM
Dept. of Pharmacology and Respiratory Medicine, KEM Hospital, Parel, Mumbai.
Rupwate
R U
RU
Desai
N K
NK
Kamat
S R
SR
D000328:Adult; D006631:Amine Oxidase (Copper-Containing) / Q000097:blood*; D001249:Asthma / Q000175:diagnosis / Q000201:enzymology*; D001792:Blood Platelets / Q000201:enzymology*; D005260:Female; D006632:Histamine / Q000097:blood*; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000097:blood*; D012016:Reference Values; D012130:Respiratory Hypersensitivity / Q000175:diagnosis / Q000201:enzymology; D012701:Serotonin / Q000097:blood*
D012701:Serotonin; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing); D008995:Monoamine Oxidase