title: Role of heat shock proteins in the effect of NMDA and KCl on cerebellar granule cells survival.
abstract: Cerebellar granule cells (CGC) die apoptotically after five days in culture (DIV) at physiological concentrations of potassium (5 mM; K5). When CGC are depolarized (K25) or treated with NMDA (150 microM) cell survival is increased. CGC changed from K25 to K5 die after 24-48 h. It is known that heat shock protein (HSP) may protect from cell death. Here, we found that cells in K5 showed an increase in HSP-70 levels after 3 DIV. Similarly, in cells changed from K25 to K5, HSP-70 levels were increased after 6 h. Neither NMDA nor K25 treatment affected HSP-70 levels from 2-7 DIV. Ethanol or thermal stress induced HSP-70, but cell survival was not affected in K5 medium. These results suggest that HSP, particularly HSP-70, are not involved in the mechanisms by which NMDA and KCl promote cell survival.
authors.affiliation: Institute of Cell Physiology, National University of Mexico, Mexico City.
authors.forename: S
authors.initials: S
authors.lastname: Alavez
authors.forename: D
authors.initials: D
authors.lastname: Pedroza
authors.forename: J
authors.initials: J
authors.lastname: Morán
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D003470:Culture Media; D000431:Ethanol / Q000494:pharmacology; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006360:Heat-Shock Proteins; D016202:N-Methylaspartate / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology*; D051381:Rats
chemical_list: D003470:Culture Media; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins; D000431:Ethanol; D016202:N-Methylaspartate; D011189:Potassium Chloride