[Use of recombinant protein E2 of the classical swine fever virus for immunization of animals].
Recombinant major surface glycoprotein E2 from virulent Shimen strain of classical swine fever virus (CSFV) has been tested for immunogenicity in animal immunization experiments. Immunization of 3-month-old piglets with 200 micrograms of recombinant protein protected the animals from lethal challenge with virulent CSFV strain. CSFV-specific antibody detection test based on competitive ELISA has been developed using the recombinant E2 protein. The test can evaluate specific antibody levels after subunit vaccination with recombinant E2 after immunization with live vaccine based on attenuated CSFV strain.
Tsibezov
V V
VV
Bogdanova
V S
VS
Zaberezhny
A D
AD
Grebennikova
T V
TV
Krivonos
A V
AV
Dudnikov
L A
LA
Kal'nov
S L
SL
Aliper
T I
TI
Nepoklonov
E A
EA
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002846:Chromatography, Affinity; D006691:Classical Swine Fever / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000276:immunology / Q000302:isolation & purification; D013552:Swine; D014759:Viral Envelope Proteins / Q000008:administration & dosage* / Q000276:immunology / Q000302:isolation & purification; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C087086:glycoprotein E2, classical swine fever virus