[Transformation of Mycoplasma hominis by plasmid pAM120 using electroporation].
Mycoplasma hominis was transformed by electroporation with plasmid pAM120 containing the transposon Tn916 that carried the tetM gene responsible for the resistance to tetracycline. The frequency of transformation was 10(-7)-10(-8) colony-forming units (CFU) per 10 micrograms of plasmid DNA. The PCR analysis of transformed DNA confirmed the transposon integration into the mycoplasma genome.
Aleksandrova
N M
NM
Research Institute of Physicochemical Medicine, Moscow, Russia.
Bevova
M R
MR
Govorun
V M
VM
D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D018274:Electroporation; D016680:Genome, Bacterial; D019535:Mycoplasma hominis / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013753:Tetracycline Resistance / Q000235:genetics; D014170:Transformation, Genetic
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S