Depressed CD40 ligand expression contributes to reduced gamma interferon production in human tuberculosis.
Expression of CD40 ligand (CD40L) correlated directly with Mycobacterium tuberculosis-stimulated gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) from tuberculosis patients and healthy tuberculin reactors. The CD40L agonist increased M. tuberculosis-induced IFN-gamma production by PBMC, and anti-CD40 or anti-CD40L antibodies reduced IFN-gamma production. CD40L expression on PBMC was reduced by exposure to B cells and to soluble factors from M. tuberculosis-infected monocytes. These findings suggest that CD40L dysregulation contributes to reduced IFN-gamma production in human tuberculosis.
Samten
B
B
Center for Pulmonary and Infectious Disease Control, University of Texas Health Center, Tyler, Texas 75710, USA.
Thomas
E K
EK
Gong
J
J
Barnes
P F
PF
D001402:B-Lymphocytes / Q000276:immunology; D019013:CD40 Antigens / Q000096:biosynthesis; D023201:CD40 Ligand; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear; D008212:Lymphocyte Depletion; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D009169:Mycobacterium tuberculosis / Q000276:immunology; D018123:Receptors, Interleukin / Q000096:biosynthesis; D053707:Receptors, Interleukin-12; D014373:Tuberculin / Q000276:immunology; D014376:Tuberculosis / Q000097:blood / Q000188:drug therapy / Q000276:immunology*
D019013:CD40 Antigens; D008562:Membrane Glycoproteins; D018123:Receptors, Interleukin; D053707:Receptors, Interleukin-12; D014373:Tuberculin; D023201:CD40 Ligand; D007371:Interferon-gamma