title: Ageing alters intrahypothalamic release patterns of vasopressin and oxytocin in rats.
abstract: The ageing process has been shown to have a profound impact on the hypothalamo-neurohypophysial system (HNS) and the hypothalamo-pituitary-adrenocortical (HPA) axis in humans as well as in rodents. Therefore, in this study, the intracerebral and peripheral release patterns of both vasopressin and oxytocin have been studied in aged male Wistar rats under basal conditions and in response to ethologically relevant stressors, using intracerebral microdialysis and chronic blood sampling techniques, respectively. Approximately a twofold higher basal release of arginine vasopressin (AVP) within the hypothalamic paraventricular nucleus (PVN), but not within the supraoptic nucleus (SON), was found in aged rats, whereas basal oxytocin (OXT) release did not differ in comparison with young rats. With increasing age the rise in intra-PVN release of both AVP and OXT was blunted in response to forced swimming. In contrast, the intra-SON release of AVP was unrelated to age. Simultaneously recorded basal secretion of both AVP and OXT from the neurohypophysis into blood was increased in aged rats, with a blunted OXT response to swim stress. Opposed to that, plasma AVP levels remained unchanged in both groups. Basal plasma levels of corticotropin (ACTH) and corticosterone were elevated in aged rats, whereas stress-elicited ACTH and corticosterone responses were indistinguishable. These results indicate age-related changes in the HNS and HPA axis with an enhanced basal activity opposed to a blunted response to stressors with increasing age. The increased basal release of AVP within the PVN suggests a role of intracerebral AVP in age-associated alterations of HPA axis regulation.
authors.affiliation: Max Planck Institute of Psychiatry, Kraepelinstr. 2-10, D-80804 Munich, Germany. Keck@mpipsykl.mpg.de
authors.forename: M E
authors.initials: ME
authors.lastname: Keck
authors.forename: M
authors.initials: M
authors.lastname: Hatzinger
authors.forename: C T
authors.initials: CT
authors.lastname: Wotjak
authors.forename: R
authors.initials: R
authors.lastname: Landgraf
authors.forename: F
authors.initials: F
authors.lastname: Holsboer
authors.forename: I D
authors.initials: ID
authors.lastname: Neumann
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000375:Aging / Q000502:physiology*; D000818:Animals; D002404:Catheterization; D003345:Corticosterone / Q000097:blood; D006982:Hypertonic Solutions / Q000494:pharmacology; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism*; D007552:Isotonic Solutions / Q000494:pharmacology; D007601:Jugular Veins; D019344:Lactic Acid / Q000097:blood; D008297:Male; D017551:Microdialysis; D009997:Osmotic Pressure; D010121:Oxytocin / Q000097:blood*; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D000077331:Ringer's Solution; D013312:Stress, Physiological / Q000378:metabolism; D013495:Supraoptic Nucleus / Q000378:metabolism; D013550:Swimming / Q000502:physiology; D014667:Vasopressins / Q000097:blood*
chemical_list: D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D014667:Vasopressins; D019344:Lactic Acid; D010121:Oxytocin; D000077331:Ringer's Solution; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone