Cytoadherence characteristics of Plasmodium falciparum isolates in Thailand using an in vitro human lung endothelial cells model.
Using an in vitro model of human lung endothelial cells, we studied different characteristics of Plasmodium falciparum isolates as potential factors for malaria severity in 2 Thai patient groups: 27 with complicated malaria and 42 with uncomplicated malaria. In regard to binding properties, no association existed between cytoadherence and rosette phenotypes (P = 0.1) and hypothrombocytemia increased the cytoadherence level (P = 0.007). Cytoadherence was significantly associated with malaria severity (P = 0.05) in contrast to rosette formation (P = 0.9). Intercellular adhesion molecule-1 and chondroitin-4-sulfate were major receptors of cytoadherence in those with complicated malaria compared with those with uncomplicated malaria (P < 10(-4)). Chondroitin-4-sulfate could act as a putative receptor for malaria complications in non-pregnant women. CD36 was the main receptor in patients with uncomplicated malaria (P < 10(-3)). Vascular cell adhesion molecule-1 and E-selectin played a minor role in 2 groups (P = 0.6). Qinghaosu derivatives were more efficient than other antimalarial drugs, but a positive correlation was observed between the 50% inhibitory concentrations of halofantrine and quinine and the number of adhesive parasitized red blood cells, suggesting their influence on cytoadherence.
Traoré
B
B
Institut National de la Santé et de la Recherche Médicale Unite 511, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Muanza
K
K
Looareesuwan
S
S
Supavej
S
S
Khusmith
S
S
Danis
M
M
Viriyavejakul
P
P
Gay
F
F
D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000962:Antimalarials / Q000494:pharmacology; D001667:Binding, Competitive / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000502:physiology; D004727:Endothelium / Q000473:pathology; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D008168:Lung / Q000473:pathology*; D016778:Malaria, Falciparum / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018512:Parasitemia; D010616:Phenanthrenes / Q000494:pharmacology; D010963:Plasmodium falciparum / Q000737:chemistry*; D011803:Quinine / Q000008:administration & dosage / Q000627:therapeutic use; D012397:Rosette Formation; D013785:Thailand
D000911:Antibodies, Monoclonal; D000962:Antimalarials; D010616:Phenanthrenes; D018799:Intercellular Adhesion Molecule-1; D002809:Chondroitin Sulfates; D011803:Quinine; C023768:halofantrine