title: Molecular cloning and embryonic expression of dFKBP59, a novel Drosophila FK506-binding protein.
abstract: A Drosophila cDNA encoding a structural homolog of mammalian FKBP59 (also identified as FKBP52), a member of the FK506-binding protein (FKBP) class of immunophilins, was isolated. The gene dFKBP59 corresponding to this cDNA has been characterized and mapped to the 30D3-4 region. The predicted amino acid sequence of this cDNA shows that the dFKBP59 protein contains one highly conserved FKBP12-like domain followed by two others with less conservation. Northern hybridization reveals that the dFKBP59 mRNA is expressed throughout the Drosophila life-cycle. In contrast to its mammalian homologs, in situ hybridization detected dFKBP59 expression in specific tissues: the lymph glands, Garland cells and oenocyte cells, which are all specialized tissues in which intensive exocytic/endocytic cycling takes place. Garland cells and oenocytes (also called Drosophila nephrocytes) function in taking up waste material from the hemolymph. Finally, I have mapped an enhancer trap element within the 5' region of dFKBP59 which may help in future studies to address the question of its function during Drosophila development.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA. stephane_zaffran@smtplink.mssm.edu
authors.forename: S
authors.initials: S
authors.lastname: Zaffran
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D005091:Exons; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics; D020104:Immunophilins / Q000235:genetics*; D017403:In Situ Hybridization; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D022021:Tacrolimus Binding Proteins
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary; D012333:RNA, Messenger; D004247:DNA; D022021:Tacrolimus Binding Proteins; D020104:Immunophilins