DNA analysis by microfabricated capillary electrophoresis device.
The LIGA (Lithographie Galvanoformung Abformung) process using synchrotron radiation lithography is applied to the microfabrication of capillary array electrophoresis (CAE) device. Laser-induced fluorescence detection system for the CAE device has been constructed by the modification of laser confocal fluorescence microscopy. DNA molecules were detected during migrating in the microchannels filled with polymer separation matrices under electric field to optimize the separation conditions for DNA analysis. Based on this observation, we demonstrated that microfabricated CAE device is realized the fast separation of DNA.
Kiba
Y
Y
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan.
Ueda
M
M
Abe
H
H
Arai
A
A
Nakanishi
H
H
Tabata
O
O
Baba
Y
Y
D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D007834:Lasers; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D004247:DNA