Preprotachykinin gene expression in goldfish brain: sexual, seasonal, and postprandial variations.
Recently, we described the complete nucleotide sequence of gamma-preprotachykinin (gamma-PPT) mRNA and the deduced amino acid sequence of the precursor on the basis of molecular cloning and sequence analysis of cDNA from goldfish brain. In the present study, gamma-PPT gene expression in the brain of goldfish was examined using quantitative Northern blot analysis. The results showed that the gamma-PPT gene is highly but differentially expressed in the olfactory bulbs, hypothalamus, and posterior brain regions. There are sexual dimorphism and seasonal variations in gamma-PPT gene expression. In addition, the postprandial changes in gamma-PPT gene expression in the olfactory bulbs and hypothalamus suggest that tachykinin peptides are involved in regulation of feeding behavior in goldfish.
Peyon
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P
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Saied
H
H
Lin
X
X
Peter
R E
RE
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D005786:Gene Expression Regulation; D006054:Goldfish; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009830:Olfactory Bulb / Q000378:metabolism; D019518:Postprandial Period; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012621:Seasons; D012727:Sex Characteristics; D015320:Tachykinins / Q000235:genetics / Q000378:metabolism*
D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin