title: Influence of streptozotocin-induced diabetes and insulin treatment on the pituitary-testicular axis during sexual maturation in rats.
abstract: Effects of streptozotocin (STZ)-diabetes and insulin treatment on the functioning of pituitary-testicular axis during sexual maturation was studied. Prepubertal (30 days old) and pubertal (50 days old) male Wistar rats were made diabetic by a single injection of STZ. A group of diabetic rats was given insulin (3U/100 g b.wt./day in 2 equally divided doses), 3 days after STZ treatment. Prepubertal and pubertal rats of all groups were killed on postnatal days 51 and 71, respectively. STZ-diabetes caused marked reduction in serum LH, FSH, prolactin, testosterone and testicular interstitial fluid testosterone as well as the activities of Leydig cellular steroidogenic enzymes (3beta-and 17beta-hydroxysteroid dehydrogenases). Insulin treatment to diabetic rats maintained these changes at control range except FSH and prolactin in prepubertal rats. The results indicate that (i) diabetes-induced steroidogenic lesions in Leydig cells represent a direct consequence of dysfunctioning of pituitary-testicular axis, (ii) the adverse effects of diabetes on pituitary-testicular functions are influenced by age of its induction and (iii) optimum insulin level is essential for the acquisition of Leydig cellular steroidogenic efficacy during sexual development.
authors.affiliation: Department of Endocrinology, Dr. A.L.M. Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai, India.
authors.forename: S
authors.initials: S
authors.lastname: Sudha
authors.forename: G
authors.initials: G
authors.lastname: Valli
authors.forename: P M
authors.initials: PM
authors.lastname: Julie
authors.forename: J
authors.initials: J
authors.lastname: Arunakaran
authors.forename: P
authors.initials: P
authors.lastname: Govindarajulu
authors.forename: K
authors.initials: K
authors.lastname: Balasubramanian
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000375:Aging; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000503:physiopathology*; D005640:Follicle Stimulating Hormone / Q000097:blood; D007328:Insulin / Q000627:therapeutic use*; D007985:Leydig Cells / Q000201:enzymology; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D010902:Pituitary Gland / Q000503:physiopathology*; D011388:Prolactin / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation; D013737:Testis / Q000503:physiopathology*; D013739:Testosterone / Q000097:blood / Q000378:metabolism
chemical_list: D007328:Insulin; D013739:Testosterone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases