Prostaglandin D synthase gene is involved in the regulation of non-rapid eye movement sleep.
To examine the function of prostaglandin (PG) D synthase (PGDS) gene, as well as endogenously produced PGD(2) in sleep regulation in vivo, we generated transgenic (TG) mice that overexpress human PGDS gene to study their sleep behavior. Although no difference was observed in the sleep/wake patterns between wild-type and TG mice, a striking time-dependent increase in non-rapid eye movement (NREM), but not in rapid eye movement (REM), sleep was observed in two independent lines of TG mice after stimulation by tail clipping. Concomitantly, the spontaneous locomotor activity of TG animals was drastically decreased in response to the tail clip. Induction of NREM sleep in TG mice was positively correlated with the PGD(2) production in the brain. Sleep, locomotion, and PGD(2) content were essentially unchanged in wild-type mice after tail clipping. The results with TG mice demonstrate the involvement of the PGDS gene in the regulation of NREM sleep.
Pinzar
E
E
Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Japan Science and Technology Corporation, 6-2-4 Furuedai, Suita, 565-0874 Osaka, Japan.
Kanaoka
Y
Y
Inui
T
T
Eguchi
N
N
Urade
Y
Y
Hayaishi
O
O
D000818:Animals; D001683:Biological Clocks; D002940:Circadian Rhythm; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D054834:Lipocalins; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009043:Motor Activity; D012894:Sleep Stages / Q000235:genetics* / Q000502:physiology; D012895:Sleep, REM / Q000235:genetics* / Q000502:physiology
D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase