An ARID family protein binds to the African swine fever virus encoded ubiquitin conjugating enzyme, UBCv1.
The NH(2)-terminal end of a protein, named SMCp, which contains an ARID (A/T rich interaction domain) DNA binding domain and is similar to the mammalian SMCY/SMCX proteins and retinoblastoma binding protein 2, was shown to bind the African swine fever virus encoded ubiquitin conjugating enzyme (UBCv1) using the yeast two hybrid system and in in vitro binding assays. Antisera raised against the SMCp protein were used to show that the protein is present in the cell nucleus. Immunofluorescence showed that although UBCv1 is present in the nucleus in most cells, in some cells it is in the cytoplasm, suggesting that it shuttles between the nucleus and cytoplasm. The interaction and co-localisation of UBCv1 with SMCp suggest that SMCp may be a substrate in vivo for the enzyme.
Bulimo
W D
WD
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, UK.
Miskin
J E
JE
Dixon
L K
LK
D000358:African Swine Fever Virus / Q000201:enzymology* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D056466:Histone Demethylases; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D008025:Ligases / Q000378:metabolism*; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D010089:Oxidoreductases, N-Demethylating; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013552:Swine; D044763:Ubiquitin-Conjugating Enzymes; D014764:Viral Proteins
D004268:DNA-Binding Proteins; D015778:Minor Histocompatibility Antigens; D011506:Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D004247:DNA; D056466:Histone Demethylases; C086877:KDM5C protein, human; C095561:KDM5D protein, human; D010089:Oxidoreductases, N-Demethylating; D011495:Histone-Lysine N-Methyltransferase; D044763:Ubiquitin-Conjugating Enzymes; C091944:ubiquitin-conjugating enzyme UBCv1, African swine fever virus; D008025:Ligases