Interaction of insulin with Burkholderia pseudomallei may be caused by a preservative.
OBJECTIVE
To re-examine the previously reported in vitro interaction of insulin with Burkholderia pseudomallei, in the light of a suggestion that the interaction may have resulted from the presence of the preservative m-cresol in commercial preparations.


METHODS
Broth culture studies of B pseudomallei were performed with and without the addition of m-cresol and various preparations of insulin.


RESULTS
Growth of B pseudomallei was inhibited by m-cresol at the concentrations found in pharmaceutical insulin preparations, and by the insulin preparation Humulin R, but not by pure insulin.


CONCLUSIONS
The results of previous experiments may have been confounded by the presence of the preservative m-cresol.
Simpson
A J
AJ
Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. fnajs@diamond.mahidol.ac.th
Wuthiekanun
V
V
D016957:Burkholderia pseudomallei / Q000187:drug effects* / Q000254:growth & development; D003408:Cresols / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007328:Insulin / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*
D003408:Cresols; D007328:Insulin; D011310:Preservatives, Pharmaceutical; C042041:3-cresol