title: Identification of macrophages at the site of peritoneal injury: evidence supporting a direct role for peritoneal macrophages in healing injured peritoneum.
abstract: OBJECTIVE
  To determine if peritoneal macrophages are present at the site of a surgical injury to the peritoneum during wound healing.
  
  
  METHODS
  Controlled research study.
  
  
  METHODS
  Academic research laboratory. EXPERIMENTAL MODEL: A murine model of peritoneal wound healing.
  
  
  METHODS
  Intraperitoneal injection of polystyrene beads 1 hour after a surgical peritoneal injury to identify peritoneal macrophages.
  
  
  METHODS
  Presence of peritoneal macrophages at the site of the healing wound as determined by intracellular polystyrene beads on transmission electron microscopy 1, 3, 7, and 14 days after injury.
  
  
  RESULTS
  Peritoneal macrophages were easily distinguished from other cell types by the phagocytosis of polystyrene beads. One day after injury, peritoneal macrophages were adherent to the wound surface. By 3 days, mesothelial cells began covering the peritoneal macrophages at the wound surface and peritoneal macrophages were identified deep within the wound. Seven days after injury, the mesothelial layer was completely reconstituted, but peritoneal macrophages persisted within the healing would below the surface mesothelium.
  
  
  CONCLUSIONS
  These data indicate that peritoneal macrophages are present at the peritoneal injury site throughout the healing interval and are consistent with these macrophages having a critical role in peritoneal wound healing.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: A F
authors.initials: AF
authors.lastname: Haney
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D004195:Disease Models, Animal; D005260:Female; D017737:Macrophages, Peritoneal / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D010537:Peritoneum / Q000293:injuries*; D011183:Postoperative Complications; D000267:Tissue Adhesions; D014945:Wound Healing