Non-surgical catheterization of the jugular vein in young pigs.
Current methods utilized for serial blood collection in the young pig are limited due to the stress and/or discomfort to which the pig is exposed. Thus, we have developed a non-surgical, minimally invasive cannulation technique which allows jugular vein catheter placement in the young pig without causing extended discomfort or stress. The procedure described is rapid (approximately 8 min/pig) and relatively simple, requiring only minimal anaesthesia for immobilization of the pig during the procedure. Routinely, 2-week-old piglets are standing in their pens within 15-20 min from initiation of the procedure. Piglets recover rapidly from the procedure and display no clinical indications of pain or discomfort. Serum concentrations of cortisol, a standard indicator of stress and/or discomfort, are asymptomatic within 2 h of completing the procedure (k = 26.14+/-3.03 ng/ml). Stress is limited to the initial immobilization of the piglets. With this technique of cannulation, we routinely maintain catheter patency for 2 days, and often for as long as 5 days.
Carroll
J A
JA
Animal Physiology Research Unit, Agricultural Research Service, United States Department of Agriculture, University of Missouri, Columbia 65211.
Daniel
J A
JA
Keisler
D H
DH
Matteri
R L
RL
D018685:Anesthetics, Inhalation; D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary*; D002405:Catheterization, Central Venous / Q000379:methods / Q000662:veterinary*; D005260:Female; D006221:Halothane; D006854:Hydrocortisone / Q000097:blood; D007601:Jugular Veins / Q000502:physiology*; D008297:Male; D013552:Swine / Q000502:physiology*
D018685:Anesthetics, Inhalation; D006221:Halothane; D006854:Hydrocortisone