Synthesis and properties of oligonucleotides containing 2'-pyrenylalkyluridine.
The synthesis, binding and fluorescence properties of oligonucleotides containing the uridine modified at the 2'-position by a pyrene group using different length of linker arm have been described. It is demonstrated that the oligonucleotides possessing a C3-amide group at the 2'-position display an enhanced signal of the pyrene monomer fluorescence upon binding to DNA segments.
Yamana
K
K
Department of Applied Chemistry, Himeji Institute of Technology, Hyogo, Japan.
Asazuma
K
K
Nakano
H
H
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014529:Uridine / Q000031:analogs & derivatives*
D009838:Oligodeoxyribonucleotides; D004247:DNA; D014529:Uridine