Identification of a novel plant virus promoter using a potyvirus infectious clone.
A putative promoter from the strawberry vein banding caulimovirus (SVBV) genome was identified by its ability to drive infection with full-length cDNA of the zucchini yellow mosaic RNA potyvirus (ZYMV). A high rate of infection was obtained with the cDNA under control of the SVBV promoter using particle bombardment technology. The SVBV promoter shows 60% homology to the cauliflower mosaic virus 35S promoter in the domain spanning the conserved motifs of CCACT (at -83) and the TATA box (at -31), to the transcription start. The 3'-end one-third of the putative promoter (328 bp) was sufficient to invoke full infectivity with the ZYMV clone, and drove transient reporter gene expression in Solanaceae and Cucurbitaceae transformed with a binary plant transformation vector. Stable expression of a reporter gene (GUS) under control of the truncated SVBV promoter was shown in transformed tobacco shoots in roots, leaves and stems.
Wang
Y
Y
Department of Virology, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Gaba
V
V
Wolf
D
D
Xia
X D
XD
Zelcer
A
A
Gal-On
A
A
D001483:Base Sequence; D019470:Biolistics; D017796:Caulimovirus / Q000235:genetics*; D018076:DNA, Complementary / Q000378:metabolism; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000821:virology; D017800:Potyvirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary