Tyrosinase-mediated formation of a reactive quinone from the depigmenting agents, 4-tert-butylphenol and 4-tert-butylcatechol.
Exposure of the skin to certain phenols or catechols such as 4-tert-butylphenol (TBP) and 4-tert-butylcatechol (TBC) may cause leukoderma. These substances are used in the polymer industry and numerous cases have been reported. Several theories of the mechanism for chemical leukoderma have been suggested. In the present study, TBP and TBC are shown to be oxidised by tyrosinase. The oxidation of TBC yields a quinone that is further investigated on its reactions with cysteine or glutathione (GSH). The products formed are isolated and identified by mass spectrometry and nuclear magnetic resonance as being 4-tert-butyl-6-S-cysteinylcatechol (cys-TBC) and 4-tert-butyl-6-S-glutathionylcatechol (GS-TBC). The reactive quinone is a strongly electrophilic substance that rapidly reacts with GSH. A depletion of the GSH defence system may give conditions where the quinone lives long enough to effect its toxic properties. The influence of the reactive tert-butylquinone on enzymatic activities is demonstrated by the inhibition of glyceraldehyde-3-phosphate dehydrogenase.
Thörneby-Andersson
K
K
Department of Dermatology, University of Lund, Sweden.
Sterner
O
O
Hansson
C
C
D000363:Agaricales / Q000201:enzymology; D000975:Antioxidants / Q000494:pharmacology*; D002396:Catechols / Q000032:analysis / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000378:metabolism / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008544:Melanocytes / Q000187:drug effects / Q000201:enzymology; D008545:Melanoma; D014442:Monophenol Monooxygenase / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010858:Pigmentation / Q000187:drug effects; D010860:Pigments, Biological / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology
D000975:Antioxidants; D002396:Catechols; D010636:Phenols; D010860:Pigments, Biological; D011809:Quinones; C038096:tert-butylcatechol; D014442:Monophenol Monooxygenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005978:Glutathione; D003545:Cysteine; C006862:butylphen