Synapse formation proceeds independently of dendritic elongation in cultured hippocampal neurons.
In central neurons, dendritic differentiation begins well after axonal elongation and is accompanied by the compartmentation of the microtubule-associated protein 2 (MAP2) in the somatodendritic domain. Whether MAP2 plays a role in the morphological and functional maturation of dendrites remains an open question and is the focus of this study. Cultured hippocampal neurons depleted of MAP2 by means of antisense oligonucleotides failed to elongate their dendrites. On the other hand, MAP2-depleted neurons were capable of receiving synapses within the same time course as their control counterparts. However, both the number of synapses per cell and the synaptic density were markedly reduced in neurons in which dendritic elongation has been impaired. Taken collectively, these results suggest that the expression of MAP2 is required for the morphological differentiation of dendrites. Dendritic elongation, however, is not a prerequisite for synapse formation in cultured hippocampal neurons.
Holgado
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Institute for Neuroscience, Northwestern University, Chicago, IL, USA.
Ferreira
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D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D003712:Dendrites / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D051381:Rats; D017470:Receptors, Glutamate / Q000378:metabolism; D013569:Synapses / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000187:drug effects / Q000648:ultrastructure; D016708:Synaptophysin / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D016376:Oligonucleotides, Antisense; D017470:Receptors, Glutamate; D016708:Synaptophysin