title: Interaction of fMet-tRNA(fMet) with the C-terminal domain of translational initiation factor IF2 from Bacillus stearothermophilus.
abstract: Analytical ultracentrifugation studies indicated that the C-terminal domains of IF2 comprising amino acid residues 520-741 (IF2 C) and 632-741 (IF2 C-2) bind fMet-tRNA with similar affinities (K(d) at 25 degrees C equal to 0.27 and 0.23 microM, respectively). Complex formation between fMet-tRNA(fMet) and IF2 C or IF2 C-2 is accompanied by barely detectable spectral changes as demonstrated by a comparison of the Raman spectra of the complexes with the calculated sum of the spectra of the individual components. These results and the temperature dependence of the K(d) of the protein-RNA complexes indicate that complex formation is not accompanied by obvious conformational changes of the components, and possibly depends on a rather small binding site comprising only a few interacting residues of both components.
authors.affiliation: Max-Delbrück-Centrum für Molekulare Medizin, D-13092, Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Krafft
authors.forename: A
authors.initials: A
authors.lastname: Diehl
authors.forename: S
authors.initials: S
authors.lastname: Laettig
authors.forename: J
authors.initials: J
authors.lastname: Behlke
authors.forename: U
authors.initials: U
authors.lastname: Heinemann
authors.forename: C L
authors.initials: CL
authors.lastname: Pon
authors.forename: C O
authors.initials: CO
authors.lastname: Gualerzi
authors.forename: H
authors.initials: H
authors.lastname: Welfle
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001411:Geobacillus stearothermophilus; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010448:Peptide Initiation Factors / Q000737:chemistry* / Q000378:metabolism*; D039881:Prokaryotic Initiation Factor-2; D011485:Protein Binding; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013816:Thermodynamics; D014461:Ultracentrifugation
chemical_list: D001426:Bacterial Proteins; D010448:Peptide Initiation Factors; D039881:Prokaryotic Initiation Factor-2; D012329:RNA, Bacterial; D012358:RNA, Transfer, Met; D016601:RNA-Binding Proteins; C089238:fMet-tRNA(fMet); C024988:tRNA, formylmethionine-