Better approaches to finding the needle in a haystack: optimizing proteome analysis through automation.
Recent evidence has demonstrated a lack of correlation between transcriptional profiles and actual protein levels in cells. Proteome analysis has therefore become indispensable and complementary to genomic analysis for an accurate picture of cellular metabolism. Although proteomics is a relatively young discipline, technology for increasing throughput in proteomic projects is rapidly being developed. The operating paradigm in proteome analysis today is a combination of two-dimensional (2-D) gel electrophoresis (for protein resolution) with mass spectrometry (for protein identification). All the intermediary steps in the procedure including gel staining, image analysis, protein spot excision, digestion and mass spectrometry can be automated to increase efficiency and save time. This report reviews the current state of the proteomics technology and discusses approaches to enhance the sensitivity of 2-D gels with fractionation techniques.
Lopez
M F
MF
VP Proteomics R&D Genomic Solutions Inc, Chelmsford, MA 01824-4171, USA. mlopez@genomicsolutions.com
D000818:Animals; D001331:Automation; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D020543:Proteome / Q000032:analysis*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D020543:Proteome