Single large DNA molecule analysis using fluorescence microscopy.
A large DNA analysis method which enable to obtain spatial information of positions of specific sequences along DNA molecule has been developed. Making use of the phenomenon that large DNA molecule is elongated stably under alternative current field in a concentrated linear polymer solution, direct observation of elongated individual lambda DNA molecules with fluorescence probes was carried out using fluorescence microscopy. Then, the spatial positions of the fluorescence spot of the probe on the DNA molecule were determined by image analysis.
Oana
H
H
Graduate School of Science, Kyoto University, Japan.
Ueda
M
M
D010582:Bacteriophage lambda; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D015246:Deoxyribonuclease EcoRI; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence / Q000379:methods; D015183:Restriction Mapping
D004279:DNA, Viral; D005456:Fluorescent Dyes; D004247:DNA; D015246:Deoxyribonuclease EcoRI