Interaction of Salmonella enterica serotype Typhimurium with dendritic cells is defined by targeting to compartments lacking lysosomal membrane glycoproteins.
Dendritic cells (DCs) play a central role in the generation of acquired immunity to infections by pathogenic microorganisms. Salmonella enterica serotype Typhimurium is known to survive and proliferate intracellularly within macrophages and nonphagocytic cells, but no data exist on how this pathogen interacts with DCs. In this report, we show the capacity of serotype Typhimurium to survive within the established mouse DC line CB1. In contrast to the case for the macrophage model, the compartments of DCs containing serotype Typhimurium are devoid of lysosomal membrane glycoproteins and the PhoPQ two-component regulatory system is not essential for pathogen intracellular survival.
García-Del Portillo
F
F
Centro de Biología Molecular "Severo Ochoa," Universidad Autónoma de Madrid-CSIC, Cantoblanco, 28049 Madrid, Spain. fgportillo@cbm.uam.es
Jungnitz
H
H
Rohde
M
M
Guzmán
C A
CA
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002451:Cell Compartmentation; D002460:Cell Line; D003713:Dendritic Cells / Q000378:metabolism / Q000382:microbiology* / Q000648:ultrastructure; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D019779:Salmonella enterica / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012703:Serotyping
D015703:Antigens, CD; D001426:Bacterial Proteins; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; C079792:PhoQ protein, Bacteria; C061064:PhoP protein, Bacteria