Thymocyte apoptosis by T-2 toxin in vivo in mice is independent of Fas/Fas ligand system.
To find whether Fas/Fas ligand (FasL) pathway is involved in T-2 toxin (T-2)-mediated thymocyte apoptosis, we used lpr/lpr (lpr) and gld/gld (gld) mice, whose Fas and FasL proteins, respectively, are functionally deficient. Based on the DNA fragmentation profile in gel electrophoresis and measurement of apoptotic cell percent by flow cytometry, the levels of thymocyte apoptosis in lpr and gld mice that had received T-2 showed that both lpr and gld mice had undergone apoptosis essentially to the same magnitude as those of corresponding wild type mice (+/+). These results strongly suggest that T-2-induced thymocyte apoptosis in vivo in mice is independent of the Fas/FasL pathway.
Murshedul
A M
AM
Department of Life Science, Faculty of Agriculture, Kagawa University, Japan.
Nagase
M
M
Yoshizawa
T
T
Sakato
N
N
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053938:DNA Fragmentation; D004591:Electrophoresis, Polyacrylamide Gel; D053222:Fas Ligand Protein; D005260:Female; D005434:Flow Cytometry; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D019463:Mice, Inbred MRL lpr; D013605:T-2 Toxin / Q000494:pharmacology*; D019014:fas Receptor / Q000378:metabolism
D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D008562:Membrane Glycoproteins; D019014:fas Receptor; D013605:T-2 Toxin