title: Effects of non-ionic surfactants on the uptake and hydrolysis of fluoresceindiacetate by alkane-oxidizing bacteria.
abstract: Biological effects of non-ionic surfactants on alkane-oxidizing bacteria were studied by assessing their influence on the uptake of prefluorochrome fluoresceindiacetate (FDA) and its intracellular hydrolysis to fluorescein. Both decreasing and increasing rates of hydrolysis as a consequence of the presence of surfactants were observed. The surfactants influenced the uptake of FDA, but not its intracellular hydrolysis. The effects of the surfactants on the uptake rate depended strongly on the structure and physico-chemical properties of the surfactants. There was no qualitative or significant quantitative difference in surfactant susceptibility between induced (alkane grown) and non-induced bacteria (acetate grown), even though the induced cells possess greater cell surface hydrophobicity.
authors.affiliation: Department of Biotechnology, Faculty of Chemistry and Biology, Norwegian University of Science and Technology, Trondheim, Norway. Per.Bruheim@chembio.ntnu.no
authors.forename: P
authors.initials: P
authors.lastname: Bruheim
authors.forename: K
authors.initials: K
authors.lastname: Eimhjellen
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D000473:Alkanes / Q000378:metabolism*; D005452:Fluoresceins / Q000378:metabolism*; D005453:Fluorescence; D020563:Gammaproteobacteria / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D006868:Hydrolysis; D010084:Oxidation-Reduction; D012240:Rhodococcus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D000473:Alkanes; D005452:Fluoresceins; D013501:Surface-Active Agents; C018506:diacetylfluorescein