Use of the monoclonal antibody M30 for detecting HSG cell apoptosis.
An immunocytochemical method using a monoclonal antibody (MoAb), M30, which reacts with the product resulting from the cleavage of cytokeratin 18 by activated caspase, was applied to detect the apoptosis of human salivary gland tumor (HSG) cells induced by epigallocatechin gallate (EGCG), gallic acid (GA) and sodium ascorbate (SA). EGCG, GA and SA dose-dependently induced HSG cell death. Immunoreactive products were significantly observed in the cytoplasm of HSG cells after treatment with all these compounds. The reactions occurred with lower concentrations of these agents and after shorter treatment times, in comparison with DNA fragmentation detected by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method. These results suggest that immunocytochemical staining with the MoAb M30 may be useful for detecting the apoptosis-inducing activities of various chemical compounds.
Kusama
K
K
Department of Oral Pathology, Meikai University School of Dentistry, Saitama, Japan. kusama@dent.meikai.ac.jp
Jiang
Y
Y
Toguchi
M
M
Ohno
J
J
Shikata
H
H
Sakashita
H
H
Sakagami
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology*; D017209:Apoptosis; D001205:Ascorbic Acid / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis; D005707:Gallic Acid / Q000494:pharmacology*; D006801:Humans; D007124:Immunoenzyme Techniques; D020287:In Situ Nick-End Labeling; D007633:Keratins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D009363:Neoplasm Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000737:chemistry / Q000473:pathology*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D005707:Gallic Acid; D007633:Keratins; D002392:Catechin; C045651:epigallocatechin gallate; D003546:Cysteine Endopeptidases; D001205:Ascorbic Acid