Physical linkage of the lysyl oxidase-like (LOXL1) gene to the PML gene on human chromosome 15q22.
A contig was constructed centered on the PML (promyelocytic leukemia) gene. Using an exon-trapping approach to identify potential genes from a pool of cosmids located 5' of the PML gene, four exons were identified that showed 100% sequence homology with the previously cloned lysyl oxidase-like (LOXL1) gene. An exon probe identified a single transcript of 2.4 kb on a multitissue Northern blot with a pattern identical to the one reported for the LOXL1 gene. Pulsed-field gel electrophoresis showed comigrating bands for both the PML cDNA and LOXL1 probes, demonstrating a physical linkage between these two genes. These data provide physical mapping information to complement the previous cytogenetic localization of LOXL1.
Goy
A
A
Molecular Biology Program of Sloan-Kettering Institute and Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Gilles
F
F
Remache
Y
Y
Zelenetz
A D
AD
D015139:Blotting, Southern; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D003001:Cloning, Molecular; D020451:Contig Mapping; D016521:Electrophoresis, Gel, Pulsed-Field; D005091:Exons / Q000235:genetics; D008040:Genetic Linkage / Q000235:genetics*; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D009687:Nuclear Proteins; D011110:Polymorphism, Genetic / Q000235:genetics; D000071156:Promyelocytic Leukemia Protein; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D025521:Tumor Suppressor Proteins
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D012333:RNA, Messenger; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human; D008249:Protein-Lysine 6-Oxidase