Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase.
A full-length cDNA encoding a novel protein was isolated and sequenced from a human hepatocellular cDNA library. This cDNA consists of 7037 base pairs and has a predicted open reading frame encoding 1924 amino acids. It possesses an RNA-helicase motif containing a DEXH-box in its amino-terminus and an RNase motif in the carboxy-terminus. From a striking homology to Caenorhabditis elegans K12H4.8, it might be a human homolog of the K12H4.8. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 14q31 near the marker D14S605.
Matsuda
S
S
Department of Molecular Pathogenesis Nagoya University School of Medicine, Japan. smatsuda@tsuru.med.nagoya.u.ac.jp
Ichigotani
Y
Y
Okuda
T
T
Irimura
T
T
Nakatsugawa
S
S
Hamaguchi
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D053487:DEAD-box RNA Helicases; D004722:Endoribonucleases; D006801:Humans; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics*; D043244:Ribonuclease III; D016415:Sequence Alignment
D004722:Endoribonucleases; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases