title: Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques.
abstract: Casein phosphopeptides (CPP) stabilize amorphous calcium phosphate (ACP) and may be used to localize ACP in dental plaque, maintaining a state of supersaturation with respect to tooth enamel, reducing demineralization and enhancing remineralization. The aim here was to investigate these effects by measuring the effect of CPP-ACP on calcium diffusion in plaque. Using Dibdin's effusion system, calcium diffusion was measured in streptococcal model plaques. This demonstrated that by providing a large number of possible binding sites for calcium, 0.1% CPP-ACP reduces the calcium diffusion coefficient by about 65% at pH 7 and 35% at pH 5. Hence, CPP-ACP binds well to plaque, providing a large calcium reservoir within the plaque and slowing diffusion of free calcium. This is likely to restrict mineral loss during a cariogenic episode and provide a potential source of calcium for subsequent remineralization. Overall, once in place, CPP-ACP will restrict the caries process.
authors.affiliation: Division of Restorative Dentistry, The Dental School, Bristol, UK. keith.rose@bristol.ac.uk
authors.forename: R K
authors.initials: RK
authors.lastname: Rose
mesh_terms: D000704:Analysis of Variance; D002118:Calcium / Q000378:metabolism*; D002130:Calcium Phosphates / Q000494:pharmacology* / Q000627:therapeutic use; D002327:Cariostatic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002364:Caseins / Q000494:pharmacology* / Q000627:therapeutic use; D003773:Dental Plaque / Q000188:drug therapy / Q000378:metabolism*; D004058:Diffusion / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010748:Phosphopeptides / Q000494:pharmacology* / Q000627:therapeutic use; D013290:Streptococcal Infections / Q000188:drug therapy / Q000378:metabolism*; D013295:Streptococcus mutans
chemical_list: D002130:Calcium Phosphates; D002327:Cariostatic Agents; D002364:Caseins; D010748:Phosphopeptides; C485828:alpha-tricalcium phosphate; C519480:amorphous calcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; D002118:Calcium