Different influence of macrophage migration inhibitory factor (MIF) in signal transduction pathway of various T cell subsets.
It has been shown that macrophage migration inhibitory factor (MIF) modulates not only macrophage functions, but also T cell functions. However, detailed analysis of the MIF function on responses of various T cell subpopulations remained to be elucidated. In this report, using a neutralizing anti-MIF monoclonal antibody (mAb) we examined MIF functions on various T cell lineages. It was shown that anti-MIF mAb inhibited antigen-specific responses of both IFN-gamma producing and IL-4 producing T cells. The inhibition appeared to be related to blockade of the signal pathway via T cell receptor (TCR) but not that via IL-2 receptor (IL-2R). However, the anti-MIF mAb showed no inhibitory effect on NK-T cell responses stimulated through TCR. These results suggest that MIF is involved in the signal pathway via TCR in mainstream T cells but not in NK-T cells.
Kitaichi
N
N
Institute of Immunological Science Hokkaido University, Department of Ophthalmology, Sapporo, Japan.
Ogasawara
K
K
Iwabuchi
K
K
Nishihira
J
J
Namba
K
K
Onoé
K
K
Konishi
J
J
Kotake
S
S
Matsuda
H
H
Onoé
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000941:Antigens / Q000276:immunology; D000954:Antigens, Surface; D002455:Cell Division; D002460:Cell Line; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D000954:Antigens, Surface; C527203:KLRB1 protein, human; D037181:Lectins, C-Type; D008263:Macrophage Migration-Inhibitory Factors; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2