Botulinum C3 enzyme changes the lactate dehydrogenase isozyme pattern of primary culture of neurons.
Changes in the lactate dehydrogenase (LDH) isozyme pattern of primary culture of neurons treated with botulinum C3 enzyme were examined in order to elucidate the functional changes accompanying the morphological change that follows ADP-ribosylation of Rho protein. Primary neurons were prepared from the cerebrum of ICR mouse embryos on day 15. Neurons were cultured in MEM with 10% fetal calf serum at 37 degrees C. In the neurons treated with C3 enzyme, a typical morphological change was observed after 24 hr, and the LDH isozyme pattern was changed after 72 hr. The ratio of H-subunit to M-subunit in LDH was decreased by C3 treatment, suggesting the induction of a state of lower intracellular oxygen consumption in neurons in the primary cultures.
Watanabe
Y
Y
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan.
Morimatsu
M
M
Syuto
B
B
D036002:ADP Ribose Transferases / Q000378:metabolism*; D000818:Animals; D001905:Botulinum Toxins; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D003014:Clostridium botulinum / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000201:enzymology*
D007527:Isoenzymes; D003994:Bucladesine; D020932:Nerve Growth Factor; D007770:L-Lactate Dehydrogenase; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins