title: Evaluation of avidin-biotin ELISA for the detection of antibodies to goat poxvirus using noninfectious diagnostic reagent.
abstract: A noninfectious soluble antigen fraction of goat poxvirus (GPV) fractionated by ammonium sulfate precipitation was tested for its suitability as coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, an avidin-biotin ELISA for the detection of GPV antibodies was optimized and evaluated using different groups of serum samples from goats with known or unknown immune status. A cut-off value higher by 60% than A492 reading of control negative sera gave a 91.8% specificity and a 94.1% sensitivity for the assay. Out of 90 goat pox-suspect sera obtained from the field, only 2 (2.2%) were found positive in the counter immunoelectrophoresis (CIE) test, which is so far the routinely used diagnostic test for goat pox, while 58 (64.4%) were positive in the avidin-biotin ELISA. The McNemar's analysis of these data showed that the avidin-biotin ELISA was significantly more efficient than the CIE test for the detection of GPV antibodies in goat sera.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon, Nainital. tvs.rao@bbsrc.ac.uk
authors.forename: T V
authors.initials: TV
authors.lastname: Rao
authors.forename: P
authors.initials: P
authors.lastname: Malik
authors.forename: D
authors.initials: D
authors.lastname: Asgola
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001360:Avidin; D001710:Biotin; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D015511:Goat Diseases / Q000276:immunology*; D006041:Goats; D008297:Male; D011212:Poxviridae / Q000276:immunology*; D011213:Poxviridae Infections / Q000276:immunology / Q000662:veterinary*; D011933:Reagent Kits, Diagnostic
chemical_list: D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D001360:Avidin; D001710:Biotin