Direct measurement of DNA by means of AFM.
We have demonstrated that the electrostatic stretch-and-positioning method is useful for the analysis of a long DNA molecule by means of atomic force microscopy (AFM). DNA molecules were stretched parallel to the field line, and immobilized onto the aluminum electrodes patterned on a glass plate. Through AFM observation, we confirmed the immobilization of individual DNA molecules, not aggregate.
Ueda
M
M
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima, Japan.
Baba
Y
Y
Iwasaki
H
H
Kurosawa
O
O
Washizu
M
M
D010582:Bacteriophage lambda; D004247:DNA / Q000737:chemistry / Q000648:ultrastructure*; D004279:DNA, Viral / Q000737:chemistry / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods; D055672:Static Electricity
D004279:DNA, Viral; D004247:DNA