Simultaneous determination of alpha, beta and gamma cyclodextrins by LC.
Cyclodextrins (CDs) can be synthesized from starch by cyclodextrin glycosyltransferase (CGTase). This enzyme produces alpha-, beta- and gamma-CDs in varying proportions. In the production of cyclodextrins, purity as well as yield are important factors. A precise and reproducible method was developed and validated for the simultaneous determination of alpha-, beta-, and gamma-CDs. Optimum separation between the three CDs was achieved using a Finepak amino column with a mobile phase consisting of acetonitrile-water (70:30, v/v) at a flow rate of 1 ml/min. Detection was carried out using a differential refractive index detector. The developed method gave good chromatographic resolution of the three components with retention times of 13.16, 16.83 and 21.74 min for alpha-, beta- and gamma-CDs, respectively. The polynomial regression data for the calibration plots exhibited good linear relationship (coefficient of correlation r = 0.9987 for alpha, r = 0.9986 for beta and r = 0.9998 for gamma-CDs) over a concentration range of 2-10 mg/ml. Statistical analysis proves that the proposed LC method is precise, reproducible and accurate for the estimation of alpha-, beta- and gamma-cyclodextrins. The method can be employed for determination of percent purity as well as estimation of process yields of the cyclodextrins during the enzymatic production.
Kinalekar
M S
MS
Department of Chemical Technology (Autonomous), University of Mumbai, Matunga, India.
Kulkarni
S R
SR
Vavia
P R
PR
D002853:Chromatography, Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D003505:Cyclodextrins; D012997:Solvents; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; C031215:betadex; C023792:gamma-cyclodextrin; C032613:alpha-cyclodextrin