Fluorescent probe and permeability to cells of isopoly (S-carboxymethyl-L-cysteine) derivative of nucleic acid bases.
Isopoly(S-carboxymethyl-L-cysteine) derivatives of nucleic acid bases were found to form stable complex with oligo-DNA in vitro. Fluorescent probed isopoly(S-carboxymethyl cysteine) derivatives of nucleic acid bases were prepared as antisense oligomers. The transfection of the oligomer into cells was carried out by HVJ-liposome method. Fluorescence was observed from the cells treated with HVJ-liposome including fluorescent probed oligomers.
Kitaoka
S
S
Materials and Life Science, Graduate School of Osaka University, Suita, Japan.
Tohnai
N
N
Inaki
Y
Y
Hatae
T
T
Tanabe
T
T
D000818:Animals; D019556:COS Cells; D002463:Cell Membrane Permeability; D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D010455:Peptides; D054735:Phosphorothioate Oligonucleotides; D018108:Respirovirus; D014162:Transfection / Q000379:methods
D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; C409812:KU5G3FL; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D010455:Peptides; D054735:Phosphorothioate Oligonucleotides; C036002:poly(S-carboxymethylcysteine)