Lateral diffusion of membrane proteins in the presence of static and dynamic corrals: suggestions for appropriate observables.
We consider the possibility of inferring the nature of cytoskeletal interaction with transmembrane proteins via optical experiments such as single-particle tracking (SPT) and near-field scanning optical microscopy (NSOM). In particular, we demonstrate that it may be possible to differentiate between static and dynamic barriers to diffusion by examining the time-dependent variance and higher moments of protein population inside cytoskeletal "corrals." Simulations modeling Band 3 diffusion on the surface of erythrocytes provide a concrete demonstration that these statistical tools might prove useful in the study of biological systems.
Brown
F L
FL
Department of Chemistry, University of California, San Diego, La Jolla, California 92093-0339 USA. flb@chem.ucsd.edu
Leitner
D M
DM
McCammon
J A
JA
Wilson
K R
KR
D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000378:metabolism; D055592:Biophysical Phenomena; D001703:Biophysics; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D004058:Diffusion; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D055095:Optics and Photonics
D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins