Differences between cation-osmotic hemolysis and filterability in exaprolol- and glutaraldehyde-treated human red blood cells.
The changes in human red blood cell microrheology in different glutaraldehyde (3.0 and 5.0 x 10(-6) mol x l(-1)) and exaprolol (2.5 and 5.0 x 10(-4) mol x l(-1)) concentrations were studied. The method of millipore filtration was compared with the method of cation-osmotic hemolysis. Both drugs prolonged the filtration time. Cation-osmotic hemolysis in glutaraldehyde-treated cells was significantly lower in comparison with the control group. On the other hand, there was a significant increase in cation-osmotic hemolysis in exaprolol-treated cells. Besides cation-osmotic hemolysis and filterability of erythrocytes, we evaluated the medium cell volume (MCV) and the medium cell hemoglobin concentration (MCHC). No changes in MCV and MCHC in glutaraldehyde-treated cells were observed. However, the MCV was significantly lower and the MCHC was significantly higher in exaprolol-treated cells. In conclusion, we suggest that the method of cation-osmotic hemolysis is more sensitive than the filtration method for determination of red blood cell microrheology.
Mojzis
J
J
Department of Pharmacology, Medical Faculty, Safárik University, Kosice, Slovak Republic. mojzis@central.medic.upjs.sk
Nicák
A
A
Linková
A
A
Jandoseková
M
M
Mirossay
L
L
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000328:Adult; D002412:Cations / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology*; D004907:Erythrocyte Deformability / Q000187:drug effects* / Q000502:physiology; D005976:Glutaral / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008875:Middle Aged; D009997:Osmotic Pressure; D011412:Propanolamines / Q000494:pharmacology*
D000319:Adrenergic beta-Antagonists; D002412:Cations; D004202:Disinfectants; D011412:Propanolamines; C012969:exaprolol; D005976:Glutaral