An SP1-like cis-element is the major DNA motif for differential expression regulation of the adipocyte amino acid transporter.
Adipocyte amino acid transporter (AAAT) is induced during the 3T3-L1 preadipocyte differentiation process. In the -1819-bp 5'-upstream flanking region of the AAAT genomic gene, six DNase I protected sites were identified by using the 3T3-L1 adipocyte nuclear extract. Results of chloramphenicol acetyltransferase (CAT) expression from the chimeric AAAT promoter-driven CAT reporter gene indicated that one protein binding site, from -68 to -26, was essential for the promoter activity. However, this protein binding site does not contain recognition sites of the transcription factors important for adipocyte differentiation, i.e., the C/EBP or PPAR family. Further analysis revealed that the DNA sequence, TTCAAGTCCCGCCCTCCGCT from -65 to -46, was the cis-element essential and partially sufficient for inducible activity of the AAAT gene promoter.
Zhu
Q
Q
Shanghai Life Sciences Research Center, Chinese Academy of Sciences, Shanghai, 200031, China.
Zhu
J
J
Liao
K
K
D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism*; D026905:Amino Acid Transport Systems; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002454:Cell Differentiation; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D018983:DNA Footprinting; D018076:DNA, Complementary / Q000378:metabolism*; D005786:Gene Expression Regulation; D015723:Gene Library; D017930:Genes, Reporter; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D016329:Sp1 Transcription Factor / Q000737:chemistry* / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection
D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D018076:DNA, Complementary; D016329:Sp1 Transcription Factor; D015500:Chloramphenicol O-Acetyltransferase