title: Lower alkaline phosphatase activity and occurrence of an abnormal hybrid intestinal/tissue non-specific isoform in Down's syndrome amniotic fluids.
abstract: Quantitative and qualitative analysis of alkaline phosphatases (AP) was performed on amniotic fluid in 59 normal pregnancies and 14 Down's syndrome (DS) pregnancies at 16, 18 and 19 weeks of gestation. In DS cases, intestinal and placental isoenzyme levels were significantly reduced (P<0.001) and the AP electrophoretic pattern was seen to be modified on polyacrylamide gel electrophoresis. A unique component was detected. After extraction and purification of the abnormal isoenzyme, peptide fragments obtained after cyanogen bromide cleavage indicated a hybrid heterodimeric AP composed of intestinal and tissue non-specific subunits, as evaluated by SDS polyacrylamide gel electrophoresis.
authors.affiliation: Laboratoire d'Enzymologie, Service Universitaire d'Hématologie (INSERM, CNRS), CHU Rangueil, 31403, Toulouse, France.
authors.forename: H
authors.initials: H
authors.lastname: Vergnes
authors.forename: J
authors.initials: J
authors.lastname: Grozdea
authors.forename: C
authors.initials: C
authors.lastname: Denier
authors.forename: G
authors.initials: G
authors.lastname: Bourrouillou
authors.forename: P
authors.initials: P
authors.lastname: Calvas
authors.forename: F
authors.initials: F
authors.lastname: De La Farge
authors.forename: P
authors.initials: P
authors.lastname: Valdiguié
authors.forename: M
authors.initials: M
authors.lastname: Calot
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000201:enzymology*; D004314:Down Syndrome / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism; D011247:Pregnancy; D011262:Pregnancy Trimester, Second
chemical_list: D007527:Isoenzymes; D000469:Alkaline Phosphatase