[Polyclonal antibodies, labeling radial nerve cells of the starfish Asterias amurensis].
The results of preliminary studies suggest that the cytoskeletal fraction of the radial nerve of the starfish Asterias amurensis contained a 32 kDa protein, which is tissue specific. This protein was isolated from the radial nerve by preparative electrophoresis and used as an antigen for raising polyclonal antibodies. When testing these antibodies on sections of the starfish tissues, it was shown that they interact only with the proteins present in the radial nerve cells. A conclusion was drawn that the raised antibodies may be used as a cell marker when studying regeneration of the nervous system in starfish.
Eliseĭkina
M G
MG
Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
Lamash
N E
NE
Bulgakov
A A
AA
Dolmatov
I Iu
IIu
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D003598:Cytoskeletal Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D011826:Radial Nerve / Q000166:cytology / Q000378:metabolism*; D013215:Starfish
D000906:Antibodies; D003598:Cytoskeletal Proteins; D009419:Nerve Tissue Proteins