Construction and characterization of a Schistosoma mansoni bacterial artificial chromosome library.
A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen.
Le Paslier
M C
MC
Fondation Jean Dausset, Paris, France.
Pierce
R J
RJ
Merlin
F
F
Hirai
H
H
Wu
W
W
Williams
D L
DL
Johnston
D
D
LoVerde
P T
PT
Le Paslier
D
D
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes; D018509:DNA, Helminth / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D015723:Gene Library; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012550:Schistosoma mansoni / Q000235:genetics*; D020079:Terminal Repeat Sequences
D015342:DNA Probes; D018509:DNA, Helminth; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases