Detection of Coxiella burnetii DNA in dental pulp during experimental bacteremia.
Colonization of dental pulp by blood-borne bacteria in the absence of previous inflammation has been hypothetized but has never been convincingly demonstrated. In order to provide convincing support for this hypothesis we attempted to detect Coxiella burnetii DNA in the dental pulp of bacteremic, intraperitoneally inoculated guinea-pigs by PCR amplification and direct sequencing of two molecular targets. Coxiella burnetii DNA was recovered from 20-50% of the animals depending on the molecular target, from 15-20 days after experimental challenge. These results demonstrated, for the first time, that dental pulp is contaminated by blood-borne bacteria and can be detected by molecular tools.
Aboudharam
G
G
Unité des Rickettsies, CNRS UPRES-A 6020, Faculté de Médecine, Université de la Méditerranée, 27 Boulevard Jean Moulin, Marseille Cedex 05, 13385, France.
Lascola
B
B
Raoult
D
D
Drancourt
M
M
D000818:Animals; D016470:Bacteremia / Q000382:microbiology*; D016997:Coxiella burnetii / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D003782:Dental Pulp / Q000382:microbiology*; D006168:Guinea Pigs; D008297:Male; D016133:Polymerase Chain Reaction; D011778:Q Fever / Q000382:microbiology*
D004269:DNA, Bacterial