title: Role of nitric oxide in the reperfusion induced injury in hyperthyroid rat hearts.
abstract: We recently reported that hyperthyroidism affects the heart response to ischemia/reperfusion. A significant tachycardia during reperfusion was associated with an increase in the oxidative stress of hearts from T3-treated animals. In the present study we checked the possible role of nitric oxide (NO) in this major stress induced by the hyperthyroid state. We compared the functional recovery from ischemia/reperfusion of Langendorff preparations from euthyroid (E) and hyperthyroid (H, ten daily intraperitoneal injections of T3, 10 microg/100 g body weight) rats, in the presence and in the absence of 0.2 mM Nomega-nitro-L-arginine (L-NNA). At the end of the ischemia/reperfusion protocol (10 min preischemic perfusion, 20 min global ischemia, 30 min reperfusion) lipid peroxidation, antioxidant capacity (CA) and susceptibility to in vitro oxidative stress were determined on heart homogenates. The main effect of hyperthyroidism on the reperfusion functional response was confirmed to be a strong tachycardic response (154% recovery at 25 min reperfusion) accompanied by a low recovery in both left ventricular diastolic pressure (LVDP) and left ventricular dP/dtmax. This functional response was associated with a reduction in CA and an increase in both lipid peroxidation and susceptibility to oxidative stress. Perfusion of hearts with L-NNA per se had small but significant negative chronotropic and positive inotropic effects on preischemic performance of euthyroid rat hearts only. More importantly, L-NNA perfusion completely blocked the reperfusion tachycardic response in the hyperthyroid rats. Concomitantly, myocardium oxidative state (lipid peroxidation, CA and in vitro susceptibility to oxidative stress) of L-NNA perfused hearts was similar to that of E animals. These results suggest that the higher reperfusion-induced injury occurring in hyperthyroid animals is associated with overproduction of nitric oxide.
authors.affiliation: Dipartimento di Fisiologia Generale ed Ambientale, Università di Napoli, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Masullo
authors.forename: P
authors.initials: P
authors.lastname: Venditti
authors.forename: C
authors.initials: C
authors.lastname: Agnisola
authors.forename: S
authors.initials: S
authors.lastname: Di Meo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006980:Hyperthyroidism / Q000150:complications* / Q000503:physiopathology; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation; D008297:Male; D017202:Myocardial Ischemia; D015428:Myocardial Reperfusion Injury / Q000209:etiology*; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D013610:Tachycardia / Q000209:etiology; D014284:Triiodothyronine / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D014284:Triiodothyronine; D019335:Nitroarginine; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase