[The effect of the composition of the culture media on bovine oocyte maturation and embryo development in vitro].
We studied the capacity of the cattle oocyte for the resumption of meiosis and achievement of metaphase II in various protein-free culture media (DMEM, TCM-199, Ham's F-10, and Ham's F-12) and the pattern of influence of the estrous serum on in vitro development of fertilized cattle oocytes, with special reference to the time of its addition to the synthetic oviduct fluid containing BSA. In the first experimental series, it was shown that the highest number of oocytes (76.1%) resumed meiosis in DMEM medium. Meiosis was not resumed in Ham's F-12. Intermediate results were obtained for TCM-199 (55.1%), which is commonly used for maturation of cattle oocytes in vitro, and for Ham's F-10 (51.7%). The oocytes reached metaphase II in DMEM at a higher rate (45.3%) than in TCM-199 or Ham's F-10 (29.4 and 8.6%, respectively). In the second experimental series, the estrous serum was added to the culture medium within 20 h (control) or 42 h (experiment) after the beginning of fertilization. The estrous serum did not inhibit the first cleavage division (the percentage of cleaving embryos did not differ reliably: 32.7 and 37.9%, respectively). However, a later serum addition to the culture medium (within 42 h after the beginning of fertilization) reliably increased the percentage of embryos that reached the blastocyst stage (6.5% in the control and 17.8% in the experiment) and the hatched blastocyst stage (2 and 9.2%, respectively).
Smetanina
I G
IG
All-Russian Institute of Physiology, Biochemistry, and Nutrition of Farm Animals, Russian Academy of Agricultural Sciences, Borovsk, Kaluga oblast, Russia.
Tatarinova
L V
LV
Krivokharchenko
A S
AS
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D002417:Cattle; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D066298:In Vitro Techniques; D008540:Meiosis / Q000187:drug effects; D008677:Metaphase / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
D001798:Blood Proteins; D016895:Culture Media, Serum-Free