title: Calcium binding to the photosystem II subunit CP29.
abstract: We have identified a Ca(2+)-binding site of the 29-kDa chlorophyll a/b-binding protein CP29, a light harvesting protein of photosystem II most likely involved in photoregulation. (45)Ca(2+) binding studies and dot blot analyses of CP29 demonstrate that CP29 is a Ca(2+)-binding protein. The primary sequence of CP29 does not exhibit an obvious Ca(2+)-binding site therefore we have used Yb(3+) replacement to analyze this site. Near-infrared Yb(3+) vibronic side band fluorescence spectroscopy (Roselli, C., Boussac, A., and Mattioli, T. A. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 12897-12901) of Yb(3+)-reconstituted CP29 indicated a single population of Yb(3+)-binding sites rich in carboxylic acids, characteristic of Ca(2+)-binding sites. A structural model of CP29 presents two purported extra-membranar loops which are relatively rich in carboxylic acids, one on the stromae side and one on the lumenal side. The loop on the lumenal side is adjacent to glutamic acid 166 in helix C of CP29, which is known to be the binding site for dicyclohexylcarbodiimide (Pesaresi, P., Sandonà, D., Giuffra, E. , and Bassi, R. (1997) FEBS Lett. 402, 151-156). Dicyclohexylcarbodiimide binding prevented Ca(2+) binding, therefore we propose that the Ca(2+) in CP29 is bound in the domain including the lumenal loop between helices B and C.
authors.affiliation: Section de Bioénergétique, Département de Biologie Cellulaire et Moléculaire, CEA/Saclay and CNRS URA 2096, 91191 Gif-sur-Yvette cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Jegerschöld
authors.forename: A W
authors.initials: AW
authors.lastname: Rutherford
authors.forename: T A
authors.initials: TA
authors.lastname: Mattioli
authors.forename: M
authors.initials: M
authors.lastname: Crimi
authors.forename: R
authors.initials: R
authors.lastname: Bassi
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007477:Ions; D045342:Light-Harvesting Protein Complexes; D008674:Metals, Rare Earth / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism; D045332:Photosystem II Protein Complex; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared; D013696:Temperature; D015018:Ytterbium / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry
chemical_list: C069660:CP29 light harvesting complex; D007477:Ions; D045342:Light-Harvesting Protein Complexes; D008674:Metals, Rare Earth; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D004024:Dicyclohexylcarbodiimide; D015018:Ytterbium; D002118:Calcium