Stable expression of functional CBP70 lectin during heat shock.
CBP70 is a glycoslylated lectin that interacts through either glycan-lectin or protein-protein interactions. In addition, depending on its cellular localization, this lectin has different partners, for example, galectin-3, an 82-kDa ligand in the nucleus, or Bcl-2 in the cytoplasm. In this study, we observed the persistence of plurilocalized lectin CBP70 after two heat-shock treatments conducted either under mild conditions, i.e., incubating the cells for 1 h at 42 degrees C then for 1, 3, 5, 7, or 9 h at 37 degrees C, or harsh conditions, i.e., incubation at 42 degrees C for 1, 2, 4, 6, 8, or 10 h. By combining the information collected from biochemical, fluorocytometric, confocal, and affinity-chromatography analyses, we concluded that CBP70 persisted in HL60 cells and its N-acetylglucosamine-binding sites remained active after all the heat-shock treatments tested. These data and the previously published findings reviewed in this report concur in supporting the hypothesis that CBP70 could function as an organizer of multimeric assembly, leading to the formation of various complexes in different cellular compartments, according to the needs of the cell.
Rousseau
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INSERM U-496, Institut Universitaire d'Hématologie, Hôpital Saint-Louis, 75475 Paris Cedex 10, France.
Felin
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Sève
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AP
D000117:Acetylglucosamine / Q000378:metabolism; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D002845:Chromatography; D003593:Cytoplasm / Q000378:metabolism; D005434:Flow Cytometry; D018922:HL-60 Cells; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism* / Q000502:physiology; D018613:Microscopy, Confocal; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D037102:Lectins; D019253:Proto-Oncogene Proteins c-bcl-2; C104787:carbohydrate-binding protein 70, human; D000117:Acetylglucosamine