Identification of epididymal stones in diverse rooster populations.
The epididymal region of the male reproductive tract is essential for sperm maturation, and dysfunction of this region results in infertility. Adult roosters have been observed to develop epididymal stones and consequently have reduced fertility. Efferent ductule cysts were first observed in White Leghorn roosters ages 18 to 26 wk. By 26 wk of age, the cysts had become solid, irregularly shaped, yellow-green stones primarily containing calcium (48%). The number and size of stones (9 to 160 microm, largest diameter) increased with age in affected males. Incidence ranged from 0 to 94% within rooster flocks surveyed. Stones have also been observed in broiler breeder roosters. Histological analysis of Leghorn and broiler breeder reproductive tracts revealed chronic inflammation with abundant interstitial mononuclear cell infiltrates. The normal, highly folded structure of efferent ductules was replaced by a thin, eroded epithelial layer with few luminal sperm. Abnormal areas were found interspersed with normal areas of epithelium. Broiler breeder male fertility trials demonstrated that birds with stones compared with normal males had reduced fertility following both natural mating (24.8+/-10.5% vs. 66.1+/-7.2%) and artificial insemination (47.8+/-16% vs. 82.0+/-6%). At 62 wk of age, testis weight (14.2+/-1.4 g vs. 20.5+/-1.2 g), daily sperm production (8.1+/-1.3 x 10(8) vs. 12.3+/-0.8 x 10(8) sperm per testis per day), and circulating testosterone concentrations (0.9+/-0.3 vs. 2.6+/-0.4 ng/mL) were all significantly reduced in males with stones. In conclusion, we are reporting a new dysfunction of the rooster reproductive tract that affects diverse bird populations and decreases fertility.
Janssen
S J
SJ
Molecular and Integrative Physiology, University of Illinois, Urbana 61802, USA.
Kirby
J D
JD
Hess
R A
RA
Rhoads
M
M
Bunick
D
D
Bailey
K L
KL
Parsons
C M
CM
Wang
H
H
Bahr
J M
JM
D000818:Animals; D002118:Calcium / Q000032:analysis; D002137:Calculi / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D002645:Chickens; D004822:Epididymis / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D007248:Infertility, Male / Q000209:etiology / Q000662:veterinary; D008297:Male; D011201:Poultry Diseases / Q000175:diagnosis* / Q000473:pathology; D012671:Seminiferous Tubules / Q000473:pathology; D013091:Spermatogenesis; D013733:Testicular Diseases / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*
D002118:Calcium