title: Microglial motility in the rat facial nucleus following peripheral axotomy.
abstract: Microglial motility was studied in living mammalian brain tissue using infrared gradient contrast microscopy in combination with video contrast enhancement and time lapse video recording. The infrared gradient contrast allows the visualization of living cells up to a depth of 60 microm in brain slices, in regions where cell bodies remain largely uninjured by the tissue preparation and are visible in their natural environment. In contrast to other techniques, including confocal microscopy, this procedure does not require any staining or labeling of cell membranes and thus guarantees the investigation of tissue which has not been altered, apart from during preparation. Microglial cells are activated and increase in number in the facial nucleus following peripheral axotomy. Thus we established the preparation of longitudinal rat brainstem slices containing the axotomized facial nucleus as a source of activated microglial cells. During prolonged video time lapse recordings, two different types of microglial cell motility could be observed. Microglial cells which had accumulated at the surface of the slice remained stationary but showed activity of the cell soma, developing pseudopods of different shape and size which undulated and which were used for phagocytosis of cell debris. Microglial phagocytosis of bacteria could be documented for the first time in situ. In contrast, ameboid microglia which did not display pseudopods but showed migratory capacity, could be observed exclusively in the depth of the tissue. Some of these cells maintained a close contact to neurons and appeared to move along their dendrites, a finding that may be relevant to the role of microglia in "synaptic stripping", the displacement of synapses following axotomy. This approach provides a valuable opportunity to investigate the interactions between activated microglial cells and the surrounding cellular and extracellular structures in the absence of staining or labeling, thus opening a wide field for the analysis of the cellular mechanisms involved in numerous pathologies of the CNS.
authors.affiliation: Department of Neurology, Technical University Aachen, Pauwelsstr. 30, D-52057 Aachen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schiefer
authors.forename: K
authors.initials: K
authors.lastname: Kampe
authors.forename: H U
authors.initials: HU
authors.lastname: Dodt
authors.forename: W
authors.initials: W
authors.lastname: Zieglgänsberger
authors.forename: G W
authors.initials: GW
authors.lastname: Kreutzberg
mesh_terms: D000818:Animals; D019771:Axotomy; D001933:Brain Stem / Q000166:cytology / Q000502:physiology; D002465:Cell Movement; D005154:Facial Nerve / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D066298:In Vitro Techniques; D008297:Male; D017628:Microglia / Q000166:cytology / Q000502:physiology*; D018715:Microscopy, Video; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017552:Solitary Nucleus / Q000166:cytology* / Q000502:physiology*