Expression of the interferon-inducible chemokines MuMig and Crg-2 following vaccinia virus infection in vivo.
MuMig (monokine induced by gamma interferon) and Crg-2 (cytokine responsive gene) are chemokines of the CXC subfamily. They share activity as T and NK cell chemoattractants. Crg-2 has been shown to be inducible by IFN, TNF, IL-1 and LPS, whereas the expression of MuMig is thought to be strictly dependent on IFN-gamma. In the present study, the kinetics of expression of the genes for MuMig and Crg-2 were analysed by northern blot analysis in organs of normal mice and in groups of gene knockout mice deficient in IFN-gamma (IFN-gamma-/-) or receptors for IFN-alpha/beta and IFN-gamma (double R-/-; DR-/-) after vaccinia virus infection. MuMig mRNA was not expressed in IFN-gamma-/- mice in all organs examined, whereas Crg-2 mRNA levels were marginally reduced. In contrast, MuMig and Crg-2 mRNA transcripts were completely abolished in the DR-/- mice.
Mahalingam
S
S
School of Human and Biomedical Sciences, Division of Science and Design, University of Canberra, ACT 2601, Australia.
Karupiah
G
G
D000818:Animals; D015152:Blotting, Northern; D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; D019743:Chemokines, CXC / Q000378:metabolism*; D005260:Female; D007371:Interferon-gamma / Q000172:deficiency; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015846:Monokines / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D017471:Receptors, Interferon / Q000172:deficiency; D013154:Spleen / Q000378:metabolism; D014599:Uterus / Q000378:metabolism; D014615:Vaccinia / Q000378:metabolism* / Q000821:virology; D014616:Vaccinia virus
D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; D019743:Chemokines, CXC; C485433:Cxcl10 protein, mouse; C485969:Cxcl9 protein, mouse; D015846:Monokines; D012333:RNA, Messenger; D017471:Receptors, Interferon; D007371:Interferon-gamma