Biomaterial-associated persistence of Staphylococcus epidermidis in pericatheter macrophages.
Biomaterial surfaces may be modified to reduce bacterial adhesion. The susceptibility in mice to Staphylococcus epidermidis infection in tissue surrounding the commonly used catheter materials-silicon elastomer (SE), polyamide (PA), and their surface-modified polyvinylpyrrolidone (PVP)-grafted derivatives, SE-PVP and PA-PVP, respectively-was assessed. Abscesses developed around SE-PVP. Around SE, PA, and PA-PVP catheters, no signs of infection were observed, although mice carrying PA-PVP developed septicemia after 14-21 days. S. epidermidis was cultured from the tissue surrounding PA-PVP segments. Cells around PA-PVP segments containing large numbers of bacteria were identified as macrophages by use of immunohistochemistry and electron microscopy. This persistence of intracellular bacteria was also observed around SE-PVP, SE, and PA catheters, although to a lesser extent. The cytokine profiles around the 4 materials were different. Implanted biomaterial induces an inflammatory response favorable to the persistence of S. epidermidis. Intracellular persistence of bacteria inside macrophages may be a pivotal process in the pathogenesis of biomaterial-associated infection.
Boelens
J J
JJ
Dept. of Pediatrics, Leiden University Medical Center, 2300 RC Leiden, The Netherlands. Boelensjj@yahoo.com
Dankert
J
J
Murk
J L
JL
Weening
J J
JJ
van der Poll
T
T
Dingemans
K P
KP
Koole
L
L
Laman
J D
JD
Zaat
S A
SA
D000818:Animals; D016470:Bacteremia / Q000209:etiology*; D001422:Bacterial Adhesion; D001672:Biocompatible Materials; D002408:Catheters, Indwelling / Q000009:adverse effects / Q000382:microbiology*; D005260:Female; D007249:Inflammation / Q000382:microbiology; D007371:Interferon-gamma / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009757:Nylons; D011205:Povidone; D012826:Silicone Elastomers; D013203:Staphylococcal Infections / Q000209:etiology; D013212:Staphylococcus epidermidis / Q000302:isolation & purification / Q000502:physiology*; D013499:Surface Properties; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D001672:Biocompatible Materials; D007375:Interleukin-1; D015850:Interleukin-6; D009757:Nylons; D012826:Silicone Elastomers; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma; D011205:Povidone