title: Effects of buffered media upon growth and alkaloid production of Catharanthus roseus hairy roots.
abstract: The influence of buffered media upon the growth and alkaloid productivity of Catharanthus roseus hairy root culture was examined. As expected, the buffers minimized shifts in the pH of the media and had slightly negative effects upon growth. The growth of the hairy roots remained optimal in unbuffered media. The specific yield of lochnericine was significantly lower in response to the addition of buffers, while tabersonine was significantly higher. In contrast, the specific yields of ajmalicine, serpentine, and horhammericine remained unchanged.
authors.affiliation: Department of Chemical Engineering, Iowa State University, Ames 50011-2230, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Morgan
authors.forename: C S
authors.initials: CS
authors.lastname: Barney
authors.forename: A H
authors.initials: AH
authors.lastname: Penn
authors.forename: J V
authors.initials: JV
authors.lastname: Shanks
mesh_terms: D000470:Alkaloids / Q000378:metabolism*; D002021:Buffers; D002478:Cells, Cultured; D003470:Culture Media; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism*; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000470:Alkaloids; D002021:Buffers; D003470:Culture Media