title: Evidence for P2X(3), P2X(4), P2X(5) but not for P2X(7) containing purinergic receptors in Müller cells of the rat retina.
abstract: P2X receptors are ligand-gated ion channels activated by ATP. They are expressed in a broad variety of tissues. To date, eight P2X receptor subunits (P2X(1)-P2X(7), P2XM) have been cloned. In spite of the considerable evidence of signaling by extracellular nucleotides in other sensory systems, only few studies have been undertaken in the retina. In earlier studies, we have demonstrated that there is mRNA expression of the P2X(2-5) and P2X(7) subunits in the rat retina. In the present study, molecular biological methods were used to investigate expression of P2X receptor mRNA in freshly isolated Müller cells (MCs) of the adult rat retina (Brown Norway). A total of 36 MCs was analyzed, employing the single-cell RT-PCR. A positive amplification signal of 11/14 for P2X(3)-mRNA, 5/10 for P2X(4)-mRNA, 3/10 for P2X(5)-mRNA and 0/8 for P2X(7)-mRNA was revealed. Additionally, the astroglial identity of the cells under studied was confirmed in 10 cases by simultaneous amplification of RT-PCR products of glutamine synthetase (GS)- and P2X-mRNA. We conclude that MCs of rat retina express ionotropic P2 receptors, which, in addition to other functions, may play a key role within the recently described long range calcium signaling and the fast direct glia-neuron interactions in the rat retina.
authors.affiliation: Clinic of Neurosurgery, Department of Experimental Neurobiology, University of Bonn, Sigmund-Freud-Strasse 25, 53105, Bonn, Germany. rjabs@mailer.meb.uni-bonn.de
authors.forename: R
authors.initials: R
authors.lastname: Jabs
authors.forename: E
authors.initials: E
authors.lastname: Guenther
authors.forename: K
authors.initials: K
authors.lastname: Marquordt
authors.forename: T H
authors.initials: TH
authors.lastname: Wheeler-Schilling
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D009457:Neuroglia / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011914:Rats, Inbred BN; D018048:Receptors, Purinergic P2 / Q000235:genetics*; D058477:Receptors, Purinergic P2X3; D058848:Receptors, Purinergic P2X4; D058485:Receptors, Purinergic P2X5; D058486:Receptors, Purinergic P2X7; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C546410:P2rx3 protein, rat; C548228:P2rx4 protein, rat; C546467:P2rx5 protein, rat; C546470:P2rx7 protein, rat; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058477:Receptors, Purinergic P2X3; D058848:Receptors, Purinergic P2X4; D058485:Receptors, Purinergic P2X5; D058486:Receptors, Purinergic P2X7