Molecular characterization of Brazilian isolates of orf virus.
Outbreaks of an epidermic disease suggesting parapox virus infections have been observed in all major herds of sheep and goats from different geographical areas of Brazil. Clinical samples (dried scabs) were collected and orf virus was isolated and characterized by electron microscopy in previous work. In order to characterize these viruses at the molecular level, a modified methodology for genomic DNA extraction directly from scabs was used and such DNA was used to derive the restriction enzyme digestion patterns for clinical samples from three distinct geographic origins. Pulsed field gel electrophoresis was used to separate restriction enzyme DNA fragments and heterogeneity among isolates from different geographic areas could be observed on stained gels. The HindIII-G DNA fragment from orf-A virus genome was cloned and hybridized to DNA of other orf virus isolates. Further heterogeneity was confirmed by these hybridizations.
Mazur
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Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Km 47 Rodovia Rio-S.Paulo, 23890-000, Seropédica, Brazil. mazur@dbbm.fiocruz.br
Ferreira
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Rangel Filho
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Galler
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D000818:Animals; D001938:Brazil / Q000453:epidemiology; D003001:Cloning, Molecular; D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D015247:Deoxyribonuclease HindIII / Q000737:chemistry; D004196:Disease Outbreaks / Q000662:veterinary; D004474:Ecthyma, Contagious / Q000453:epidemiology / Q000821:virology*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D015511:Goat Diseases / Q000453:epidemiology / Q000821:virology*; D006041:Goats; D008854:Microscopy, Electron / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D009923:Orf virus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D012756:Sheep
D015342:DNA Probes; D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII