Formation of 8-nitroguanine from 2'-deoxyguanosine by NO/O2 system.
We investigated the reaction of 2'-deoxyguanosine (dGuo) with NO/O2 gas mixture under physiological condition and detected 8-nitroguanine, which is known as a novel DNA lesion caused by peroxynitrite (ONOO-). The yield increased with increase in the ratio of O2 and pH. The reaction mechanism is discussed.
Suzuki
T
T
Institute of Advanced Energy, Kyoto University, Uji, Japan.
Yamada
M
M
Kanaori
K
K
Tajima
K
K
Morii
T
T
Makino
K
K
D003849:Deoxyguanosine; D006147:Guanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D008956:Models, Chemical; D009566:Nitrates; D009569:Nitric Oxide; D010084:Oxidation-Reduction; D010100:Oxygen
C095838:8-nitroguanine; D009566:Nitrates; C039642:peroxynitric acid; D009569:Nitric Oxide; D006147:Guanine; D003849:Deoxyguanosine; D010100:Oxygen