Structural identification of the lipopolysaccharide O-antigen produced by Yersinia enterocolitica serotype O:28.
The structure of the O-antigenic polysaccharide (O-PS) component of the lipopolysaccharide produced by Yersinia enterocolitica serotype O:28 has been elucidated. From chemical methods involving glycose analysis, periodate oxidation, methylation and the use of one- and two-dimensional NMR spectroscopy, the O-PS was found to be a polymer of repeating branched hexasaccharide units composed of L-rhamnose (four parts), 2-acetamido-2-deoxy-D-glucose (one part), and 2-acetamido-2-deoxy-D-galacturonic acid (one part) having the following structure:
Perry
M B
MB
Institute for Biological Sciences, National Research Council, Ottawa, Ontario, Canada. malcolm.perry@nrc.ca
MacLean
L L
LL
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D015008:Yersinia enterocolitica / Q000737:chemistry*
D019081:O Antigens