Development and assessment of ELISA for serodiagnosis of HIV-infection.
A home-made ELISA for serodiagnosis of HIV-infection was developed. It made use of the HIV viral lysate to coat on ELISA microplates. The purpose was to establish an ELISA for serodiagnosis of HIV-infection. The newly-developed ELISA, "H-ELISA", was applied to test 792 samples of HIV-positive serum as confirmed by Western blot. All 792 samples were positive by H-ELISA. It was also applied to test 540 samples of normal sera obtained from different laboratories in Srinagarind Hospital. A number 530 normal sera was negative, 8 samples were positive and confirmed by Western blot and 2 samples were false positive. It was concluded that the H-ELISA possessed 100% sensitivity with a false positive rate of 2/532(0.38%). The H-ELISA, the cost/test was less than 5 bahts, appears to be promising for substitution of imported commercial kits.
Thongkrajai
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P
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand.
Kaewmart
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R
D015492:AIDS Serodiagnosis / Q000191:economics / Q000379:methods*; D000372:Agglutination Tests; D015153:Blotting, Western; D003363:Cost Control; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity