title: The polar-lipid composition of the sphingolipid-producing bacterium Flectobacillus major.
abstract: Polar lipids comprise about 90% of the total chloroform-methanol extractable lipids of the Gram-negative, fresh-water, ring-forming bacterium Flectobacillus major FM and consist of at least 10 constituents. These are aminophosphosphingolipids, 2-N-(2'-D-hydroxy-13'-methyltetradecanoyl)-15-methyl-4(E)-hexad ecasph ingenyl-1-phosphoethanolamine (36.8% of the total polar lipids) and its 2'-deoxy derivative (3.7%); sulfonic-acid analogues of ceramide, 2-D-(2'-D-hydroxy-13'-methyltetradecanoyl)amino-3-D-hydroxy-15-met hyl hexadecane-1-sulfonic acid (18.1%) and its 2'-deoxy derivative (3. 5%); a lipoamino acid, N-[3-D-(15'-methylhexadecanoyloxy)-15-methylhexadecanoyl]-gl ycine (3. 7%); a lipodipeptide, N-¿N'-[3"-D-(15"'-methylhexadecanoyloxy)-15"-methylhexadecanoyl ]glycy l¿-L-serine (7.8%); 1,2-diacyl-sn-glycero-3-phosphoethanolamine (7. 7%), 1,2-diacyl-3-alpha-D-galactopyranosyl-sn-glycerol (2.9%); ceramide phospho-myo-inositol (4.9%), and a previously described unusual glycosphingolipid, 7-deoxy-7-amino-D-manno-heptulosonopyranosyl (1-hydroxycarbonyl-6-deoxy-6-amino-alpha-D-mannopyranosyl) ceramide (10.9%); the last two lipids contain only 15-methyl-4(E)-hexadecasphingenine as a long-chain base. The sole structural type of amide-bound fatty acids in the sphingolipids, including the sulfonic-acid analogues, is iso-15:0, either non-hydroxylated or hydroxylated at 2-C, whereas 15-methylhexadecanoic acid is the major ester-bound fatty acid in the remaining lipids.
authors.affiliation: Research Centre 'Hydrobios' of Ministry of Health, ul. Kosmonavtov 18, korp. 2, Moscow, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Batrakov
authors.forename: A E
authors.initials: AE
authors.lastname: Mosezhnyi
authors.forename: A O
authors.initials: AO
authors.lastname: Ruzhitsky
authors.forename: V I
authors.initials: VI
authors.lastname: Sheichenko
authors.forename: D I
authors.initials: DI
authors.lastname: Nikitin
mesh_terms: D005591:Chemical Fractionation; D002855:Chromatography, Thin Layer; D006088:Gram-Negative Aerobic Bacteria / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D015394:Molecular Structure; D013107:Sphingolipids / Q000302:isolation & purification / Q000378:metabolism*; D013109:Sphingomyelins / Q000032:analysis
chemical_list: D013107:Sphingolipids; D013109:Sphingomyelins; C060281:ceramide phosphoethanolamine