Radiosynthesis of 3'-deoxy-3'-[(18)F]fluorothymidine: [(18)F]FLT for imaging of cellular proliferation in vivo.
A reliable radiosynthesis of 3'-deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT) has been developed based on [(18)10 mCi (370 MBq) of radiochemically pure [(18)1 Ci/micromol (37 GBq/micromol) at EOS within 100 min and in 13% radiochemical yield (end of bombardment (EOB); 7% end of synthesis (EOS)). [(18)F]FLT has been designed as a new positron emission tomography imaging agent for visualizing cellular proliferation in vivo based on the metabolism of thymidine.
Grierson
J R
JR
Research Imaging Laboratory, University of Washington Medical Center, Seattle, Washington 98195, USA. grierson@u.washington.edu
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D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D015224:Dideoxynucleosides / Q000138:chemical synthesis*; D005462:Fluorine Radioisotopes; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D008968:Molecular Conformation; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D013056:Spectrophotometry, Ultraviolet; D013936:Thymidine / Q000378:metabolism; D014961:X-Ray Diffraction
D015224:Dideoxynucleosides; D005462:Fluorine Radioisotopes; D007202:Indicators and Reagents; D019275:Radiopharmaceuticals; C002854:alovudine; D013936:Thymidine