Secure cranial-mount intrathecal cannulation - a spinal administration technique in the rat.
In order to identify an acute spinally mediated pharmacological effect of a bioactive substance, without incurring untoward supraspinal effects, it is necessary to administer the agent locally onto the spinal cord. The procedure delineated herein presents a modern technique to install a stable, permanent indwelling thecal cannulae with a cranially mounted aperture, and details a simple, repeatable administration system. These methods facilitate a quick, noninvasive spinal drug microadministration that is most useful for differentiation of the locus of pharmacological action without the behavioral disruption associated with other administration methodologies.
Schechter
J B
JB
Department of Neurobiology and Pharmacology, Northeastern Ohio Universities College of Medicine, P.O. Box 95, Rootstown, OH 44272-0095, USA.
Crisp
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D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000494:pharmacology; D000758:Anesthesia; D000818:Animals; D004305:Dose-Response Relationship, Drug; D007278:Injections, Spinal / Q000295:instrumentation*; D008845:Microinjections; D009020:Morphine / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000502:physiology*; D013238:Stereotaxic Techniques / Q000295:instrumentation*
D000701:Analgesics, Opioid; D009020:Morphine