Evaluation of avidin-biotin ELISA for the detection of antibodies to goat poxvirus using noninfectious diagnostic reagent.
A noninfectious soluble antigen fraction of goat poxvirus (GPV) fractionated by ammonium sulfate precipitation was tested for its suitability as coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, an avidin-biotin ELISA for the detection of GPV antibodies was optimized and evaluated using different groups of serum samples from goats with known or unknown immune status. A cut-off value higher by 60% than A492 reading of control negative sera gave a 91.8% specificity and a 94.1% sensitivity for the assay. Out of 90 goat pox-suspect sera obtained from the field, only 2 (2.2%) were found positive in the counter immunoelectrophoresis (CIE) test, which is so far the routinely used diagnostic test for goat pox, while 58 (64.4%) were positive in the avidin-biotin ELISA. The McNemar's analysis of these data showed that the avidin-biotin ELISA was significantly more efficient than the CIE test for the detection of GPV antibodies in goat sera.
Rao
T V
TV
Division of Virology, Indian Veterinary Research Institute Campus, Mukteswar-Kumaon, Nainital. tvs.rao@bbsrc.ac.uk
Malik
P
P
Asgola
D
D
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001360:Avidin; D001710:Biotin; D003378:Counterimmunoelectrophoresis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D015511:Goat Diseases / Q000276:immunology*; D006041:Goats; D008297:Male; D011212:Poxviridae / Q000276:immunology*; D011213:Poxviridae Infections / Q000276:immunology / Q000662:veterinary*; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic; D001360:Avidin; D001710:Biotin