Time-shared X(omega(1))-half-filter for improved sensitivity in subspectral editing.
Experiments with X-half-filter elements allow the separation of the resonances from protons bound and unbound to a spin X into different subspectra. This Communication presents a modified half-filter element where the filter delay is simultaneously used for chemical shift labeling and scalar coupling evolution in a semi-constant time experiment. The filter element is demonstrated with a (1)H NOESY spectrum of a 28.5-kDa 2:1 complex between the uniformly (13)C-labeled N-terminal domain of Escherichia coli arginine repressor and operator DNA.
Andersson
P
P
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, S-171 77, Sweden.
Otting
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G
D002247:Carbon Isotopes; D004269:DNA, Bacterial / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004926:Escherichia coli; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012680:Sensitivity and Specificity
D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins