title: The role of cytotoxic necrotizing factor-1 in colonization and tissue injury in a murine model of urinary tract infection.
abstract: Cytotoxic necrotizing factor-1 (CNF1) is commonly found in Escherichia coli isolates from patients with urinary tract infection (UTI). To determine whether CNF1 is an important UTI virulence factor we compared the ability of a clinical E. coli UTI isolate and a CNF1-negative mutant of that isolate to colonize and induce histological changes in the urinary tract in a murine model of ascending UTI. We found no evidence that the mutant strain was attenuated.
authors.affiliation: Research Service (151), Department of Veterans Affairs, 10 North Greene St., Baltimore, MD 21201, USA. dejohnso@umaryland.edu
authors.forename: D E
authors.initials: DE
authors.lastname: Johnson
authors.forename: C
authors.initials: C
authors.lastname: Drachenberg
authors.forename: C V
authors.initials: CV
authors.lastname: Lockatell
authors.forename: M D
authors.initials: MD
authors.lastname: Island
authors.forename: J W
authors.initials: JW
authors.lastname: Warren
authors.forename: M S
authors.initials: MS
authors.lastname: Donnenberg
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D003603:Cytotoxins / Q000378:metabolism*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000254:growth & development* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology; D029968:Escherichia coli Proteins; D005260:Female; D006801:Humans; D051379:Mice; D008808:Mice, Inbred CBA; D014551:Urinary Tract / Q000382:microbiology / Q000473:pathology; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000473:pathology
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; C060296:cytotoxic necrotizing factor type 1