title: A facile synthesis of DNA oligomers with modified backbones via the phosphoramidite method without nucleoside base protection.
abstract: A convenient synthesis of DNA oligomers with modified back bones including phosphorothioates and phosphotriesters has been developed on the basis of the phosphoramidite strategy without nucleoside base protection. In this synthesis, a new, convenient method for the preparation of N-free-5'-O-dimethoxytrityl-2'-deoxyribonucleoside by the direct O-selective dimethoxytritylation of the parent nucleosides has also been disclosed.
authors.affiliation: Graduate School of Human Informatics, Nagoya University, Chikusa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kataoka
authors.forename: R
authors.initials: R
authors.lastname: Kawai
authors.forename: Y
authors.initials: Y
authors.lastname: Hayakawa
mesh_terms: D000577:Amides; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010756:Phosphoric Acids; D013873:Thionucleotides
chemical_list: D000577:Amides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010756:Phosphoric Acids; D013873:Thionucleotides; D004247:DNA; C011067:phosphoramidic acid