Response of MHC class-1 antigen on rat glioma cells to cytokines.
BACKGROUND
Enhanced MHC class-1 expression has potentially been correlated with the increased susceptibility of tumor cells to a T cell-mediated immune response. We examined the immunomodulatory effects of some cytokines on MHC class-1 expression in rat glioma cell lines.


METHODS
Using three rat glioma cell lines (RG2, C6, 9L), the immunomodulatory effects of cytokines (rhIL-2, rmIL-4, rmGM-CSF, rrIFN-gamma) on the expression of MHC class-1 antigen were evaluated using immunohistochemical and flow cytometric analyses.


RESULTS
Varying baseline levels of MHC class-1 antigen were confirmed in each glioma cell line. Stimulation with rrIFN-gamma consistently increased the expression of MHC-1 antigen in all three of the rat glioma cell lines. On the other hand, MHC class-1 expression was not affected by stimulation with rhIL-2, rmIL-4 or rmGM-CSF, even in combination.


CONCLUSIONS
rrIFN-gamma can upregulate MHC class-1 expression in rat glioma cells. Our data suggest that rrIFN-gamma may be able to exert antitumor activity against gliomas in vivo.
Oshiro
S
S
Department of Neurosurgery, School of Medicine, Fukuoka University, Japan.
Fukushima
T
T
Tomonaga
M
M
Black
K L
KL
D000818:Animals; D001932:Brain Neoplasms / Q000276:immunology*; D016207:Cytokines / Q000494:pharmacology*; D005434:Flow Cytometry; D005910:Glioma / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007150:Immunohistochemistry; D051381:Rats
D016207:Cytokines; D015395:Histocompatibility Antigens Class I