Inhibitory effects of carotenoids on the invasion of rat ascites hepatoma cells in culture.
The effects of carotenoids--alpha-carotene, beta-carotene, lycopene, beta-cryptoxanthin, zeaxanthin, lutein, canthaxanthin, astaxanthin--on the invasion of rat ascites hepatoma AH109A cells were investigated by co-culturing the hepatoma cells with rat mesentery-derived mesothelial cells (M-cells). All the carotenoids examined inhibited AH109A invasion in a dose-dependent manner up to 5 microM. Cancer cells previously cultured with hypoxanthine (HX) and xanthine oxidase (XO) showed a highly invasive activity. Carotenoids, 5 microM of beta-carotene and astaxanthin, suppressed this reactive oxygen species-potentiated invasive capacity by simultaneously treating AH109A cells with the carotenoids, HX and XO. These results suggest that the antioxidative property of these carotenoids may be involved in their anti-invasive action.
Kozuki
Y
Y
Department of Applied Biological Science, Tokyo Noko University, Fuchu, Japan.
Miura
Y
Y
Yagasaki
K
K
D000818:Animals; D016644:Canthaxanthin / Q000494:pharmacology; D002338:Carotenoids / Q000494:pharmacology*; D018920:Coculture Techniques; D065366:Cryptoxanthins; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D008114:Liver Neoplasms, Experimental / Q000473:pathology* / Q000517:prevention & control; D014975:Lutein / Q000494:pharmacology; D000077276:Lycopene; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D051381:Rats; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives / Q000494:pharmacology
D065366:Cryptoxanthins; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene; D016644:Canthaxanthin; C005948:astaxanthine; D000077276:Lycopene; D014975:Lutein