A simultaneous ex vivo model of embryogenesis: II. Vasculogenesis.
Vasculogenesis was simultaneously studied with embryogenesis in in ovo chick embryo culture, which was harvested at 40 hours. Endodermal cells and vascular endothelial cells were studied using a new combination of stains, immunohistochemistry (for nuclei and basement membrane) and NADPH-diaphorase activity in whole-mounts, paraffin sections and etched semithin sections. The model can be used for the study of developmental process of blood vessels as well as embryonic physiology of blood vessels vis-a-vis organogenesis in response to different angiogenic agents, drug trials, cancer therapy by angiostatic chemicals/radiations and toxins. Considering that vasculogenesis/angiogenesis as one of the fundamental phenomena in physiology, pathophysiology, toxicology and pharmacology of developmental sciences, the model in developing embryo is presented.
Dey
N
N
Institute of Pathology (ICMR), New Delhi.
Iyengar
B
B
D000818:Animals; D001808:Blood Vessels / Q000196:embryology* / Q000378:metabolism; D002642:Chick Embryo / Q000196:embryology* / Q000378:metabolism; D046508:Culture Techniques; D004707:Endoderm / Q000166:cytology; D007150:Immunohistochemistry; D008954:Models, Biological; D009252:NADPH Dehydrogenase / Q000378:metabolism; D018919:Neovascularization, Physiologic
D009252:NADPH Dehydrogenase