title: Neural reorganisation in porcine vein grafts: a potential role for endothelin-1.
abstract: Vein grafts are associated with adventitial remodelling which may influence innervation of the graft. Since there is also evidence that endothelin-1 (ET-1) plays a role in the adventitial remodelling process, we investigated neural distribution in porcine vein grafts 1 and 6 months after implantation, as well as the localisation of immunoreactive ET-1 and its receptors in the same tissues. Saphenous vein-carotid artery interposition grafting was performed in Landrace pigs. One and 6 months after surgery, vein grafts and ungrafted saphenous veins were excised; neural tissue and ET-1 were identified by immunocytochemistry and ET receptors were identified using in vitro autoradiography. In ungrafted saphenous veins, abundant perivascular nerves were located in the outer region of the media with only a few paravascular nerves in the adventitia. In vein grafts at 1 month after implantation, there was almost complete depletion of perivascular nerves in the media. In contrast, in the neoadventitia, there was an emphatic appearance of large paravascular nerve bundles and a marked increase in small paravascular nerves. These changes were more pronounced at 6 months after surgery, although the principal changes had occurred within 1 month. Immunoreactive ET-1 (index of ET-1 content) was associated with paravascular nerve bundles, appearing as a dark, dense ring at the perineurium. Furthermore, within the nerve bundles, positive ET-1 immunoreactivity was associated with positive alpha-actin staining, indicating that ET-1 is associated with (neural) microvessels. Also, dense 125I-labelled BQ3020 (ET(B)-selective) binding to nerve bundles was observed, indicating the presence of ET(B) receptor subtypes. ET(A) receptor subtypes were not detected in neural tissue. These data demonstrate neural reorganisation in vein grafts and indicate that ET-1 content and binding may play a role in this process. The functional consequences of these changes on neointima formation, a major cause of vein graft failure, remain to be determined.
authors.affiliation: Departments of Molecular Pathology and Clinical Biochemistry and Surgery, Royal Free Hospital and University College Medical School, London, UK.
authors.forename: M R
authors.initials: MR
authors.lastname: Dashwood
authors.forename: R
authors.initials: R
authors.lastname: Gibbins
authors.forename: D
authors.initials: D
authors.lastname: Mehta
authors.forename: M
authors.initials: M
authors.lastname: Bashar Izzat
authors.forename: G D
authors.initials: GD
authors.lastname: Angelini
authors.forename: J Y
authors.initials: JY
authors.lastname: Jeremy
mesh_terms: D000818:Animals; D001345:Autoradiography; D017536:Carotid Artery, Common / Q000601:surgery; D019332:Endothelin-1 / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D017466:Receptors, Endothelin / Q000378:metabolism; D012501:Saphenous Vein / Q000294:innervation* / Q000378:metabolism / Q000637:transplantation*; D013552:Swine
chemical_list: D019332:Endothelin-1; D017466:Receptors, Endothelin