Differential immunoglobulin E and cytokine responses in BALB/c and C57Bl/6 mice during repeated infections with blood-stage Plasmodium chabaudi malaria.
Repeated blood-stage Plasmodium chabaudi chabaudi AS challenge infections in BALB/c and C57Bl/6 mice result in increased serum immunoglobulin (Ig) E levels and splenic cytokine production. The genetic background of the host influences both the cytokine response as well as the development of IgE antibodies. BALB/c mice showed high interleukin (IL)-4 secretion from splenocytes after in-vitro stimulation with malaria antigen after repeated P. chabaudi challenges and this was closely followed by higher levels of total IgE. Despite slightly elevated serum IgE levels, splenocytes from C57Bl/6 mice did not secrete any detectable IL-4 but produced interferon (IFN)-gamma in response to malaria antigen-stimulation in vitro. These data suggest that induction of IgE antibodies during murine malaria infection is genetically regulated.
Helmby
H
H
Department of Immunology, Stockholm University, S-109 61 Stockholm, Sweden.
Troye-Blomberg
M
M
D000818:Animals; D000953:Antigens, Protozoan / Q000008:administration & dosage; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood; D066298:In Vitro Techniques; D015847:Interleukin-4 / Q000378:metabolism; D007700:Kinetics; D008213:Lymphocyte Activation; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018512:Parasitemia / Q000276:immunology*; D016788:Plasmodium chabaudi / Q000276:immunology; D013045:Species Specificity; D013154:Spleen / Q000276:immunology
D000953:Antigens, Protozoan; D016207:Cytokines; D015847:Interleukin-4; D007073:Immunoglobulin E