Determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories using reverse-phase HPLC.
Fluocortolone and its esters are synthetic corticosteroids used topically in the treatment of various skin disorders. A method that can be successfully used for the separation and determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories was developed. This method is based on reverse-phase HPLC on Supelcosil LC-18 (25 cm x 4.6 mm, 5 microns), using methanol-acetonitrile-water-glacial acetic acid (17:46:37:0.4 v/v/v/v) as mobile phase at a flow rate of 3.0 ml/min. Detection was carried out using a UV detector at 238 nm. The method developed was validated, and calibration curves were established dependent on peak area. The validated ranges for fluocortolone pivalate and fluocortolone hexanoate are 15-305 micrograms/ml (r = 0.9995) and 15-315 micrograms/ml (r = 0.9996), respectively. The limits of detection and the limits of quantification for both esters were also determined.
Cudina
O
O
Department of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Belgrade, Yugoslavia. fpharma@eunet.yu
Brboric
J
J
Vujic
Z
Z
Radulovic
D
D
Vladimirov
S
S
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005448:Fluocortolone / Q000032:analysis*; D005938:Glucocorticoids; D004364:Pharmaceutical Preparations / Q000737:chemistry; D013488:Suppositories / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D004364:Pharmaceutical Preparations; D013488:Suppositories; D005448:Fluocortolone