Interaction of cefotetan and the metallo-beta-lactamases produced in Aeromonas spp. and in vitro activity.
Aeromonas spp. are increasingly being recognized as human pathogens. The presence of metallo-beta-lactamases in these organisms represents a potential problem in antimicrobial therapy. Mechanism-based inactivators of beta-lactamases are used to overcome the resistance of clinical pathogens to beta-lactam antibiotics, but no clinical useful inhibitors of the metallo-beta-lactamases are presently known. Studying the interaction between cefotetan and Aeromonas spp. producing metallo-beta-lactamase activity, we observed that cefotetan behaved as a transient inactivator for both the crude extracts of Aeromonas strains and the purified enzymes from Aeromonas hydrophila AE036 and Aeromonas schubertii MNSA20. The direct hydrolysis of cefotetan showed that it was a poor substrate for both purified enzymes. In view of the minimum inhibitory concentrations, cefotetan shows to be a useful antimicrobial agent against Aeromonas spp.
Quiroga
M I
MI
University of Misiones, Argentina.
Franceschini
N
N
Rossolini
G M
GM
Gutkind
G
G
Bonfiglio
G
G
Franchino
L
L
Amicosante
G
G
D000333:Aeromonas / Q000187:drug effects* / Q000201:enzymology*; D016980:Aeromonas hydrophila / Q000187:drug effects / Q000201:enzymology; D001426:Bacterial Proteins; D015313:Cefotetan / Q000494:pharmacology*; D002513:Cephamycins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008667:Metalloproteins / Q000187:drug effects / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000187:drug effects / Q000378:metabolism*
D001426:Bacterial Proteins; D002513:Cephamycins; D004791:Enzyme Inhibitors; D008667:Metalloproteins; D065093:beta-Lactamase Inhibitors; D015313:Cefotetan; D001618:beta-Lactamases; C063912:carbapenemase