title: Evaluation of argatroban and DUP 714 as anticoagulants for blood gas, electrolyte and ionized calcium analyses.
abstract: The objective of this study was to determine if the thrombin inhibitors Argatroban and DUP 714 could anticoagulate whole blood without influencing the analyses of blood gases, electrolytes, ionized calcium or CO-oximetry. The anticoagulant potency of DUP 714 (0.5-68 micromol/l) and Argatroban (1.5-390 micromol/l) was evaluated using the activated partial thromboplastin time (APTT), prothrombin time (PT) and whole blood clot time (WBCT). APTT and the PT were measured using a Behring Fibrintimer. APTT was found to be more sensitive to prolongation by both of the thrombin inhibitors than were the PT or WBCT assays. DUP 714 was found to a more potent anticoagulant than Argatroban. DUP 714 anticoagulated specimens (>2.2 micromol/l) did not clot for at least 2 days, whereas Argatroban preserved specimens (390 micromol/l) clotted within 5.5 h of collection. No statistically significant changes in the measurement of pH, PCO2, PO2, Na, K, ionized calcium, oxyhaemoglobin, deoxyhaemoglobin, methaemoglobin or carboxyhaemoglobin (measured using a Corning 288 Blood Gas/Electrolyte Analyzer and a Coming 270 CO-oximeter) were detected in DUP 714 (34 micromol/l) or Argatroban (390 micromol/l) anticoagulated whole blood specimens. In conclusion, DUP 714 and Argatroban are suitable anticoagulants for preserving blood prior to blood gas and electrolyte analyses.
authors.affiliation: Department of Pharmacology, University of Saskatchewan, Saskatoon, Canada. lyonmar@duke.usask.ca
authors.forename: M E
authors.initials: ME
authors.lastname: Lyon
authors.forename: S R
authors.initials: SR
authors.lastname: Harding
authors.forename: S N
authors.initials: SN
authors.lastname: Oosman
authors.forename: A W
authors.initials: AW
authors.lastname: Lyon
mesh_terms: D000991:Antithrombins; D001120:Arginine / Q000031:analogs & derivatives; D001784:Blood Gas Analysis / Q000379:methods*; D001896:Boron Compounds; D002118:Calcium / Q000032:analysis* / Q000097:blood; D002624:Chemistry, Clinical / Q000379:methods; D004573:Electrolytes / Q000032:analysis* / Q000097:blood; D005069:Evaluation Studies as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009842:Oligopeptides; D010092:Oximetry / Q000379:methods; D010875:Pipecolic Acids; D013449:Sulfonamides
chemical_list: D000991:Antithrombins; D001896:Boron Compounds; D004573:Electrolytes; D009842:Oligopeptides; D010875:Pipecolic Acids; D013449:Sulfonamides; C066061:acetylphenylalanyl-prolyl-boroarginine; D001120:Arginine; C031942:argatroban; D002118:Calcium