title: Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice.
abstract: To define a gene expression mechanism, it is often advantageous to use a reporter gene and transgenic mouse. The lacZ reporter gene is particularly useful for studies of the cis-regulatory element for tissue-specific expression in transgenic mice because of the ease of the enzyme assay and visualization on sections. In this report, we describe our method for examining the cis-regulatory element in transgenic mice, including choice of the lacZ gene, generation of transgenic mice, and analysis of beta-galactosidase activity.
authors.affiliation: Tsukuba Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Tsukuba, Japan. e2-takahashi@hhc.eisai.co.jp
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Miyamoto
authors.forename: N
authors.initials: N
authors.lastname: Kajiwara
authors.forename: K
authors.initials: K
authors.lastname: Furuya
authors.forename: K
authors.initials: K
authors.lastname: Yanai-Taniguchi
authors.forename: F
authors.initials: F
authors.lastname: Sugiyama
authors.forename: K
authors.initials: K
authors.lastname: Yagami
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D020865:Calcium Channels, P-Type / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D017930:Genes, Reporter / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D013194:Staining and Labeling; D014018:Tissue Distribution; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D020865:Calcium Channels, P-Type; D001616:beta-Galactosidase