A sensitive docetaxel assay in plasma by solid-phase extraction and high performance liquid chromatography-UV detection: validation and suitability in phase I clinical trial pharmacokinetics.
We have developed a specific and sensitive method aiming at docetaxel (Taxotere) determination in plasma of treated patients. This involved solid-phase extraction of 1 ml of plasma onto carboxylic acid (CBA) grafted silica cartridges followed by reversed-phase liquid chromatography with UV detection. The best selectivity was obtained through the use of C18 Uptisphere as stationary phase. The low limit of quantitation obtained (LOQ: 5 ng/ml) allowed measurements of docetaxel up to 24 hours after one-hour infusions with low dosages of drug (60 mg/m2). The method was applied successfully to monitor docetaxel plasma levels within two protocols associating fixed dosages of either methotrexate or gemcitabine with escalating doses of Taxotere.
Ardiet
C J
CJ
Laboratory of Pharmacokinetics, Centre Léon Bérard, Lyon, France.
Tranchand
B
B
Zanetta
S
S
Guillot
A
A
Bernard
E
E
Peguy
M
M
Rebattu
P
P
Droz
J P
JP
D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000097:blood; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D000971:Antineoplastic Combined Chemotherapy Protocols; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003841:Deoxycytidine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000097:blood; D000077143:Docetaxel; D006801:Humans; D008727:Methotrexate / Q000008:administration & dosage / Q000097:blood; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000097:blood; D015203:Reproducibility of Results; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D043823:Taxoids; D000093542:Gemcitabine
D000964:Antimetabolites, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D012997:Solvents; D043823:Taxoids; D003841:Deoxycytidine; D000077143:Docetaxel; D017239:Paclitaxel; D008727:Methotrexate; D000093542:Gemcitabine