Evaluation of assays for the detection of autoantibodies to the ribosomal P proteins.
Sera from systemic lupus erythematosus patients that had antibodies to the ribosomal P proteins were compared in several different assays. The enzyme-linked immunosorbent assay (ELISA) method was compared to the Western immunoblotting method using either affinity purified human or bovine ribosomal P proteins. All 30 normal sera had no significant reactivity with these antigens. The most sensitive test was the ELISA using the human P protein, where 31/32 patients were positive (97%). The assay with bovine proteins in ELISA yielded 28/32 (88%) positive results. Immunoblotting with either bovine or human P protein was equally effective with 30/32 (94%) positive. An ELISA incorporating human P proteins is a more sensitive assay for clinical diagnosis than an ELISA with the bovine protein. Immunoblotting is a sensitive method, but is less convenient and is not quantitative. The ELISA with the human protein appears to be the method of choice.
Rayno
K
K
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA.
Reichlin
M
M
D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D001426:Bacterial Proteins; D015153:Blotting, Western; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D012269:Ribosomal Proteins / Q000276:immunology*
D001323:Autoantibodies; D001426:Bacterial Proteins; C095564:Map protein, Staphylococcus aureus; D012269:Ribosomal Proteins