Changes in susceptibility of Pseudomonas aeruginosa to gatifloxacin and carbapenem in an in vitro urinary bladder model.
To study the changes of bacterial susceptibility during exposure to antimicrobials, the respective minimal inhibitory concentrations (MICs) of 50 colonies from each of various bacterial test strains were determined in an experimental system using an in vitro urinary bladder model. The MICs of gatifloxacin and meropenem for Pseudomonas aeruginosa strains were not homogeneous before exposure to the drugs. The MICs of the strains examined reflected only that of their dominant population. When starting from 10(7)CFU/ml, the bacterial count was determined serially during exposure to gatifloxacin or meropenem in therapeutic urinary concentrations. The MIC of the less susceptible population of each strain was changed serially to a higher one. The more susceptible strains were eradicated before the change of the MIC. The bacteria originally consisted of strains having heterogeneous susceptibility to drugs, in which major strains had susceptibility expressed by the MIC and minor ones had a higher or lower MIC. This heterogeneity may be responsible for the change of susceptibility in strains after exposure to the drugs.
Takahashi
S
S
Department of Urology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Hirose
T
T
Sano
M
M
Nishimura
M
M
Matsukawa
M
M
Mikami
M
M
Tsukamoto
T
T
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001437:Bacteriuria / Q000188:drug therapy / Q000382:microbiology*; D015780:Carbapenems / Q000494:pharmacology*; D024841:Fluoroquinolones; D000077734:Gatifloxacin; D006801:Humans; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013845:Thienamycins / Q000494:pharmacology
D000890:Anti-Infective Agents; D015780:Carbapenems; D024841:Fluoroquinolones; D013845:Thienamycins; D000077731:Meropenem; D000077734:Gatifloxacin