title: The gene encoding P27 lipoprotein and a putative antibiotic-resistance gene form an operon in Mycobacterium tuberculosis and Mycobacterium bovis.
abstract: P27 is an antigenic membrane lipoprotein synthesized by members of the Mycobacterium tuberculosis complex. Northern blotting and RT-PCR experiments indicated that the genes encoding P27 and a putative antibiotic transporter (P55) form an operon. A promoter region was identified and characterized by deletion analysis in Mycobacterium smegmatis. Two transcription initiation points were mapped in Mycobacterium bovis BCG by primer extension analysis to 76 bp and 87 bp upstream of the ATG initiation codon. Putative -10 and -35 promoter consensus sequences associated with these showed 66% similarity to previously identified mycobacterial promoters. These results suggest that the P27/P55 operon is transcribed from two promoters in M. bovis BCG.
authors.affiliation: Instituto de Biotecnologı́a1 and Instituto de Patobiologı́a2, CICV-INTA, PO Box 77, Castelar, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Bigi
authors.affiliation: Instituto de Biotecnologı́a1 and Instituto de Patobiologı́a2, CICV-INTA, PO Box 77, Castelar, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Alito
authors.affiliation: Instituto de Biotecnologı́a1 and Instituto de Patobiologı́a2, CICV-INTA, PO Box 77, Castelar, Argentina.
authors.forename: M I
authors.initials: MI
authors.lastname: Romano
authors.affiliation: Instituto de Biotecnologı́a1 and Instituto de Patobiologı́a2, CICV-INTA, PO Box 77, Castelar, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Zumarraga
authors.affiliation: Instituto de Biotecnologı́a1 and Instituto de Patobiologı́a2, CICV-INTA, PO Box 77, Castelar, Argentina.
authors.forename: K
authors.initials: K
authors.lastname: Caimi
authors.affiliation: Instituto de Biotecnologı́a1 and Instituto de Patobiologı́a2, CICV-INTA, PO Box 77, Castelar, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Cataldi
mesh_terms: D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000235:genetics*; D009163:Mycobacterium bovis / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009876:Operon
chemical_list: D008074:Lipoproteins; C110121:P27 protein, Mycobacterium