title: The comparative content of the mitochondrial phosphoglyceroyl-ATP-containing polymer, purinogen, in rat tissues.
abstract: Here, we describe an assay for the tissue content of purinogen, a highly phosphorylated labile polymer containing ATP and phosphoglycerate found in the mitochondrial intermembrane space. We report the purinogen content (as adenine nucleotide equivalents) of rat heart and, for the first time, of rat liver, kidney, brain and mixed skeletal muscle. The findings show that purinogen contains very significant proportions of cell adenine nucleotides ranging from 25% of the free pool in brain and skeletal muscle to 135% of it in kidney. The evidence that purinogen may form a controlled intracellular reservoir of inorganic phosphate is briefly discussed.
authors.affiliation: Regulatory Mechanisms Group, Department of Biochemistry, University College London, Gower Street, London, UK.
authors.forename: P
authors.initials: P
authors.lastname: Freeman
authors.forename: B
authors.initials: B
authors.lastname: Patel
authors.forename: J
authors.initials: J
authors.lastname: Mowbray
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005994:Glycerophosphates / Q000032:analysis*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000227:Adenine Nucleotides; D005994:Glycerophosphates; C049248:phosphoglyceroyl-ATP; D000255:Adenosine Triphosphate