Monoclonal antibody based dot-enzyme linked immunosorbent assay (Dot-ELISA) and agar gel precipitation test (AGPT) for identification of Newcastle disease virus (NDV).
Two mouse monoclonal antibodies (MAbs), viz. 2B7 and 2 D10 raised against haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus (NDV) were used to identify several other field isolates and vaccine strains of NDV. These MAbs reacted specifically with all the NDV strains/isolates in Dot-ELISA whereas, only MAb 2D10 reacted with all the NDV strains/isolates in agar gel precipitation test. These two tests employing the MAbs were standardised for rapid diagnosis and identification of NDV.
Swain
P
P
Division of Virology, Indian Veterinary Research Institute, Nainital, India.
Verma
K C
KC
Kataria
J M
JM
Mohanty
S K
SK
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006389:Hemagglutinins, Viral / Q000276:immunology; D051379:Mice; D009439:Neuraminidase / Q000276:immunology; D009522:Newcastle disease virus / Q000276:immunology / Q000302:isolation & purification*; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D009439:Neuraminidase