Bovine respiratory syncytial virus: first serological evidence in Uruguay.
Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease in calves resulting in a substantial economic loss for the cattle industry worldwide. In order to determine the presence of BRSV in Uruguay, an immunoenzymatic test was set up, using a recombinant BRSV nucleocapsid (N) protein as the antigen. The N protein was produced in Sf9 insect cells by a recombinant baculovirus expressing the N protein. Serum samples collected from one hundred cattle from four different geographic regions of Uruguay were analyzed. Antibodies against the N protein of BRSV were detected in 95% of the serum samples analyzed. These results show for the first time the presence of BRSV antibodies and suggest a widespread BRSV infection in the cattle population of Uruguay.
Costa
M
M
Departamento de Técnicas Nucleares Aplicadas, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
García
L
L
Yunus
A S
AS
Rockemann
D D
DD
Samal
S K
SK
Cristina
J
J
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019590:Nucleocapsid Proteins / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D018357:Respiratory Syncytial Virus Infections / Q000662:veterinary*; D018112:Respiratory Syncytial Virus, Bovine / Q000302:isolation & purification*; D018411:Spodoptera; D014583:Uruguay
D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins