Induction of apoptosis by hexamethylene bisacetamide is p53-dependent associated with telomerase activity but not with terminal differentiation.
The present study investigated the relationships amongst apoptosis, terminal differentiation and telomerase activity in human colon carcinoma cells. We found that hexamethylene bisacetamide (HMBA) induced apoptosis in human colon carcinoma LoVo cells harbouring wild-type p53 but not in SW1116 cells harbouring mutant p53. HMBA reduced telomerase activity in both colon carcinoma cells but it did not induce differentiation in the colon carcinoma cells. Taken together, our results suggest that HMBA can induce apoptosis via a p53-dependent pathway, but apoptosis and terminal differentiation may be separately regulated in LoVo cells. Inhibition of telomerase activity may activate apoptosis through a p53-dependent mechanism.
Zhang
Z
Z
Department of Anatomy, The University of Hong Kong, Li Shu Fan Building, SAR, Hong Kong, PR China.
Liong
E C
EC
Lau
T Y
TY
Leung
K M
KM
Fung
P C
PC
Tipoe
G L
GL
D000081:Acetamides / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D019098:Telomerase / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*
D000081:Acetamides; D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53; D019098:Telomerase; D000469:Alkaline Phosphatase; C014026:hexamethylene bisacetamide