title: Extracorporeal plateletpheresis induces the interaction of activated platelets with white blood cells.
abstract: OBJECTIVE
  In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells.
  
  
  METHODS
  Analysis of platelet-leukocyte interaction was performed using multiparameter, three-color flow cytometry.
  
  
  RESULTS
  Over the duration of the procedure, there was an increase in the surface expression of CD62p (P-selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly.
  
  
  CONCLUSIONS
  This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.
authors.affiliation: Transfusion Medicine/Transplantation Immunology, University Hospital Eppendorf, University of Hamburg, Hamburg, Germany. gutensohn@uke.uni-hamburg.de
authors.forename: K
authors.initials: K
authors.lastname: Gutensohn
authors.forename: A
authors.initials: A
authors.lastname: Alisch
authors.forename: W
authors.initials: W
authors.lastname: Krueger
authors.forename: N
authors.initials: N
authors.lastname: Kroeger
authors.forename: P
authors.initials: P
authors.lastname: Kuehnl
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood; D018941:Antigens, CD19 / Q000097:blood; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D017252:CD3 Complex / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood; D002450:Cell Communication; D005112:Extracorporeal Circulation / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000166:cytology; D019007:P-Selectin / Q000096:biosynthesis / Q000097:blood; D015539:Platelet Activation; D010980:Platelet Membrane Glycoproteins; D010983:Plateletpheresis; D013601:T-Lymphocytes / Q000276:immunology; D060149:Tetraspanin 30
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; D017252:CD3 Complex; C554586:CD63 protein, human; D015815:Cell Adhesion Molecules; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30