title: Isolation of Borna disease virus from human brain tissue.
abstract: Serological and molecular epidemiological studies indicate that Borna disease virus (BDV) can infect humans and is possibly associated with certain neuropsychiatric disorders. We examined brain tissue collected at autopsy from four schizophrenic patients and two healthy controls for the presence of BDV markers in 12 different brain regions. BDV RNA and antigen was detected in four brain regions of a BDV-seropositive schizophrenic patient (P2) with a very recent (2 years) onset of disease. BDV markers exhibited a regionally localized distribution. BDV RNA was found in newborn Mongolian gerbils intracranially inoculated with homogenates from BDV-positive brain regions of P2. Human oligodendroglia (OL) cells inoculated with brain homogenates from BDV-positive gerbils allowed propagation and isolation of BDVHuP2br, a human brain-derived BDV. Virus isolation was also possible by transfection of Vero cells with ribonucleoprotein complexes prepared from BDV-positive human and gerbil brain tissues. BDVHuP2br was genetically closely related to but distinct from previously reported human- and animal-derived BDV sequences.
authors.affiliation: Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060-0815, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Shoya
authors.forename: T
authors.initials: T
authors.lastname: Nakaya
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Tomonaga
authors.forename: K
authors.initials: K
authors.lastname: Iwahashi
authors.forename: K
authors.initials: K
authors.lastname: Ameno
authors.forename: N
authors.initials: N
authors.lastname: Momiyama
authors.forename: H
authors.initials: H
authors.lastname: Taniyama
authors.forename: T
authors.initials: T
authors.lastname: Sata
authors.forename: T
authors.initials: T
authors.lastname: Kurata
authors.forename: J C
authors.initials: JC
authors.lastname: de la Torre
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D001891:Borna disease virus / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D001921:Brain / Q000473:pathology / Q000821:virology*; D016022:Case-Control Studies; D002522:Chlorocebus aethiops; D005260:Female; D005849:Gerbillinae; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012559:Schizophrenia / Q000473:pathology / Q000821:virology*; D017422:Sequence Analysis, DNA; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D012367:RNA, Viral