A new method of quantification of pipemidic-acid by capillary zone electrophoresis with end-column amperometric detection.
Capillary zone electrophoresis was employed for the determination of pipemidic acid using an end-column amperometric detection with a carbon fiber microdisk array electrode, at a constant potential of -1.10 V vs. saturated calomel electrode. The optimum conditions of separation and detection were 1.2 x 10(-4) mol/LNaOAc - 8.8 x 10(-4) mol/ LHOAc for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time. The limit of detection was 1.05 x 10(-7) mol/L or 189 amol (S/N=3). The relative standard deviation was 0.31% for the migration time and 2.0% for the electrophoretic peak current. The method was applied to determining pipemidic acid in human serum.
Jin
W
W
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China. wenrujin@jn-public.sd.cninfo.net
Yu
D
D
Dong
Q
Q
Ye
X
X
D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood; D002021:Buffers; D002244:Carbon; D000077482:Carbon Fiber; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D017669:Mercury Compounds; D010876:Pipemidic Acid / Q000032:analysis* / Q000097:blood; D011786:Quality Control; D015203:Reproducibility of Results
D000890:Anti-Infective Agents; D002021:Buffers; D000077482:Carbon Fiber; D007202:Indicators and Reagents; D017669:Mercury Compounds; D002244:Carbon; C015728:calomel; D010876:Pipemidic Acid