title: Identification, characterization, and crystal structure of the Omega class glutathione transferases.
abstract: A new class of glutathione transferases has been discovered by analysis of the expressed sequence tag data base and sequence alignment. Glutathione S-transferases (GSTs) of the new class, named Omega, exist in several mammalian species and Caenorhabditis elegans. In humans, GSTO 1-1 is expressed in most tissues and exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities characteristic of the glutaredoxins. The structure of GSTO 1-1 has been determined at 2.0-A resolution and has a characteristic GST fold (Protein Data Bank entry code ). The Omega class GSTs exhibit an unusual N-terminal extension that abuts the C terminus to form a novel structural unit. Unlike other mammalian GSTs, GSTO 1-1 appears to have an active site cysteine that can form a disulfide bond with glutathione.
authors.affiliation: Molecular Genetics Group and Human Genetics Group, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory 2601, Australia.
authors.forename: P G
authors.initials: PG
authors.lastname: Board
authors.forename: M
authors.initials: M
authors.lastname: Coggan
authors.forename: G
authors.initials: G
authors.lastname: Chelvanayagam
authors.forename: S
authors.initials: S
authors.lastname: Easteal
authors.forename: L S
authors.initials: LS
authors.lastname: Jermiin
authors.forename: G K
authors.initials: GK
authors.lastname: Schulte
authors.forename: D E
authors.initials: DE
authors.lastname: Danley
authors.forename: L R
authors.initials: LR
authors.lastname: Hoth
authors.forename: M C
authors.initials: MC
authors.lastname: Griffor
authors.forename: A V
authors.initials: AV
authors.lastname: Kamath
authors.forename: M H
authors.initials: MH
authors.lastname: Rosner
authors.forename: B A
authors.initials: BA
authors.lastname: Chrunyk
authors.forename: D E
authors.initials: DE
authors.lastname: Perregaux
authors.forename: C A
authors.initials: CA
authors.lastname: Gabel
authors.forename: K F
authors.initials: KF
authors.lastname: Geoghegan
authors.forename: J
authors.initials: J
authors.lastname: Pandit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology; D018360:Crystallography, X-Ray; D005260:Female; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008322:Mammals; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D016324:Sequence Tagged Sites; D013379:Substrate Specificity; D014158:Transcription, Genetic
chemical_list: D007527:Isoenzymes; D005982:Glutathione Transferase