Gamma-interferon bioassay for detection of bovine tuberculosis in cattle: kinetics of production and dose response in whole blood culture.
Stimulation with Mycobacterium bovis PPD sensitised lymphocytes (whole blood or peripheral blood lymphocytes) results in release of gamma-interferon that can be detected by simple bioassay. The optimum concentration of bovine PPD was 20 micrograms ml and the optimum incubation period was 24 hr for maximum production of gamma-interferon in whole blood culture (128 units/ml) and peripheral blood culture (64 units/ml).
Bhatia
S
S
Immunology Section, Indian Veterinary Research Institute, Izatnagar, India.
Das
S K
SK
D000818:Animals; D001681:Biological Assay; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000032:analysis* / Q000096:biosynthesis; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D014373:Tuberculin / Q000008:administration & dosage / Q000276:immunology; D014380:Tuberculosis, Bovine / Q000175:diagnosis* / Q000276:immunology
D014373:Tuberculin; D007371:Interferon-gamma