Changes in anti-oxidant enzymes and lipid peroxidation in hyaline membrane disease.
This study was conducted to elucidate the changes in key antioxidant enzymes e.g. Superoxide dismutase (SOD), Catalase and Glutathione peroxidase (GPx) along with lipid peroxidation (LPO) in preterm newborns having hyaline membrane disease (HMD) and thus to find out role of free radicals mediated injury in this disease. Twenty one preterm appropriate for gestational age newborns were included in the study. Eleven of them had hyaline membrane disease and ten were controls without any disease. Status of superoxide dismutase, glutathione peroxidase and catalase, the three main antioxidant enzymes and lipid peroxidation was monitored at 12-24 hours of age. SOD and catalase were found significantly elevated in cases having hyaline membrane disease along with significantly more lipid peroxidation. It is evident that free radicals result in the induction of the antioxidant enzymes; however, the elevated enzymes are unable to counteract the high concentration of the free radicals which are being produced in the diseased cases and leads to increase in lipid peroxidation in hyaline membrane disease. It is concluded that free radicals play a significant role in hyaline membrane disease and the preterm newborns have ability to induce antioxidant enzymes in response to oxidative stress.
Singh
S K
SK
Department of Pediatrics and Biochemistry, Lady Hardinge Medical College and Associated, Kalawati Saran Children's Hospital, New Delhi.
Tandon
A
A
Kumari
S
S
Ravi
R N
RN
Ray
G N
GN
Batra
S
S
D002374:Catalase / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D006819:Hyaline Membrane Disease / Q000175:diagnosis / Q000503:physiopathology*; D007223:Infant; D007231:Infant, Newborn; D015227:Lipid Peroxidation / Q000502:physiology*; D008168:Lung / Q000503:physiopathology; D008297:Male; D013482:Superoxide Dismutase / Q000097:blood*
D005609:Free Radicals; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase