title: Toward artificial ribonucleases: design and synthesis of 2'-O-methyloligonucleotides with a terpyridine-copper(II) complex.
abstract: In order to construct an antisense 2'-O-methyloligonucleotide with an internal terpyridine-Cu(II) complex, a novel synthon was synthesized. This synthon was a 2'-deoxy-uridine-3'-phosphoramidite derivative with a terpyridine group at the 5'-O and a dimethoxytritylated hydroxypropyl group at the C-5. The antisense agent cleaved the RNA site-specifically and in a moderate yield.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Furukawa
authors.forename: Y
authors.initials: Y
authors.lastname: Komatsu
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: H
authors.initials: H
authors.lastname: Inoue
mesh_terms: D001483:Base Sequence; D003300:Copper; D015195:Drug Design; D007202:Indicators and Reagents; D020320:Oligoribonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry; D009942:Organometallic Compounds; D012260:Ribonucleases; D013379:Substrate Specificity; D014529:Uridine / Q000031:analogs & derivatives*
chemical_list: D007202:Indicators and Reagents; D020320:Oligoribonucleotides, Antisense; D009942:Organometallic Compounds; D003300:Copper; D012260:Ribonucleases; D014529:Uridine