Hybrid scanning ion conductance and scanning near-field optical microscopy for the study of living cells.
We have developed a hybrid scanning ion conductance and scanning near-field optical microscope for the study of living cells. The technique allows quantitative, high-resolution characterization of the cell surface and the simultaneous recording of topographic and optical images. A particular feature of the method is a reliable mechanism to control the distance between the probe and the sample in physiological buffer. We demonstrate this new method by recording near-field images of living cells (cardiac myocytes).
Korchev
Y E
YE
Division of Medicine, Imperial College School of Medicine, MRC Clinical Sciences Centre, Hammersmith Campus, London W12 0NN, United Kingdom. Y.Korchev@ic.ac.uk
Raval
M
M
Lab
M J
MJ
Gorelik
J
J
Edwards
C R
CR
Rayment
T
T
Klenerman
D
D
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D066298:In Vitro Techniques; D007477:Ions; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D009206:Myocardium / Q000648:ultrastructure; D011817:Rabbits
D007477:Ions