Transcript levels of the nuclear-encoded respiratory genes in rice decrease by oxygen deprivation: evidence for involvement of calcium in expression of the alternative oxidase 1a gene.
We investigated the effect of oxygen on the expressions of respiratory genes encoded in the nuclear and mitochondrial genomes of rice (Oryza sativa L.). Hypoxic treatment decreased the transcript levels of nuclear-encoded, but not mitochondrial-encoded respiratory genes. The effects of ruthenium red (an inhibitor of Ca(2+) fluxes from organelles) and/or CaCl(2) on plants under hypoxic conditions suggested that Ca(2+) is a physiological transducer of a low-oxygen signaling pathway for expression of the alternative oxidase 1a gene (AOX1a), but not for expressions of genes involved in the cytochrome respiratory pathway, in rice.
Tsuji
H
H
Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Nakazono
M
M
Saisho
D
D
Tsutsumi
N
N
Hirai
A
A
D000693:Anaerobiosis; D002118:Calcium / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D002467:Cell Nucleus / Q000235:genetics; D019069:Cell Respiration / Q000187:drug effects / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D018745:Genome, Plant; D024101:Mitochondrial Proteins; D008954:Models, Biological; D012275:Oryza / Q000166:cytology / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism* / Q000494:pharmacology; D010940:Plant Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D012430:Ruthenium Red / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D000099188:Alternative Oxidase
D002118:Calcium; D004272:DNA, Mitochondrial; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010100:Oxygen; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D012430:Ruthenium Red; D000099188:Alternative Oxidase