title: Lateral diffusion of membrane proteins in the presence of static and dynamic corrals: suggestions for appropriate observables.
abstract: We consider the possibility of inferring the nature of cytoskeletal interaction with transmembrane proteins via optical experiments such as single-particle tracking (SPT) and near-field scanning optical microscopy (NSOM). In particular, we demonstrate that it may be possible to differentiate between static and dynamic barriers to diffusion by examining the time-dependent variance and higher moments of protein population inside cytoskeletal "corrals." Simulations modeling Band 3 diffusion on the surface of erythrocytes provide a concrete demonstration that these statistical tools might prove useful in the study of biological systems.
authors.affiliation: Department of Chemistry, University of California, San Diego, La Jolla, California 92093-0339 USA. flb@chem.ucsd.edu
authors.forename: F L
authors.initials: FL
authors.lastname: Brown
authors.forename: D M
authors.initials: DM
authors.lastname: Leitner
authors.forename: J A
authors.initials: JA
authors.lastname: McCammon
authors.forename: K R
authors.initials: KR
authors.lastname: Wilson
mesh_terms: D001457:Anion Exchange Protein 1, Erythrocyte / Q000737:chemistry / Q000378:metabolism; D055592:Biophysical Phenomena; D001703:Biophysics; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism; D004058:Diffusion; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D055095:Optics and Photonics
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins