title: Resveratrol-type oligostilbenes from Iris clarkei antagonize 20-hydroxyecdysone action in the Drosophila melanogaster B(II) cell line.
abstract: Bioassay-guided high-performance liquid chromatography analysis of a MeOH extract of Iris clarkei seeds yielded the resveratrol-type oligomeric stilbenes, ampelopsin B and alpha-viniferin, which antagonize the action of 20-hydroxyecdysone; with a 20-hydroxyecdysone concentration of 50 nM, the ED50 values were 33 microM and 10 microM, respectively. The structures of these compounds were determined by spectroscopic analysis, notably ultraviolet, liquid secondary ion mass spectrometry and modern one- and two-dimensional nuclear magnetic resonance techniques.
authors.affiliation: Department of Biological Sciences, University of Exeter, UK.
authors.forename: K
authors.initials: K
authors.lastname: Keckeis
authors.forename: S D
authors.initials: SD
authors.lastname: Sarker
authors.forename: L
authors.initials: L
authors.lastname: Dinan
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects*; D004441:Ecdysterone / Q000819:agonists / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010944:Plants / Q000737:chemistry*; D011863:Radioimmunoassay; D000077185:Resveratrol; D012639:Seeds / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D001572:Benzofurans; D005419:Flavonoids; D013267:Stilbenes; C106407:ampelopsin; D004441:Ecdysterone; C064176:alpha-viniferin; D000077185:Resveratrol