Detection and identification of labeled DNA by surface enhanced resonance Raman scattering.
The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.
Graham
D
D
Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, United Kingdom.
Mallinder
B J
BJ
Smith
W E
WE
D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D009841:Oligonucleotides / Q000032:analysis / Q000737:chemistry; D012235:Rhodamines; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods; D013096:Spermine / Q000378:metabolism; D013997:Time Factors
D015342:DNA Probes; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012235:Rhodamines; D013096:Spermine