Quantitative detection of a DNA ligase reaction on a quartz-crystal microbalance.
We report here kinetic analyses of DNA ligation by using a DNA-immobilized quartz-crystal microbalance (QCM), which enables in situ real-time monitoring of both the binding of ligase and ligation reaction on DNA strands, as mass changes.
Okahata
Y
Y
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Masunaga
Y
Y
Matsuno
H
H
Furusawa
H
H
D017122:Bacteriophage T4 / Q000201:enzymology; D001483:Base Sequence; D011088:DNA Ligases / Q000378:metabolism*; D007700:Kinetics; D008832:Microchemistry / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D011791:Quartz
D009838:Oligodeoxyribonucleotides; D011791:Quartz; D011088:DNA Ligases