Synergistic role of cAMP and IP(3) in corticotropin-releasing hormone-induced cell shape changes in invertebrate immunocytes.
Immunocytes from the mollusc Mytilus galloprovincialis express corticotropin-releasing hormone (CRH) receptor subtype (CRH-R1 and CRH-R2)-like mRNAs. Using computer-assisted microscopic image analysis, we have found that exogenous CRH provokes changes in the cellular shape of immunocytes, and that this response is extracellular Ca(2+)-dependent. The various inhibitors of transduction signaling pathways, i.e. suramin sodium, 2', 5'-dideoxyadenosine, neomycin sulfate, calphostin C, H-89, and wortmannin, completely or partially inhibit these changes. The present findings demonstrate that PKA, PKC, and PKB/Akt are involved in CRH-induced cell shape changes in immunocytes, and that the cellular effect of CRH needs the synergistic action of the two second messengers, cAMP and IP(3).
Malagoli
D
D
Department of Animal Biology, University of Modena and Reggio Emilia, via Berengario, 14-41100, Modena, Italy. ottaviani.enzo@unimo.it
Franchini
A
A
Ottaviani
E
E
D000818:Animals; D020013:Calcium Signaling; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D015342:DNA Probes; D004357:Drug Synergism; D006458:Hemolymph / Q000276:immunology; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D007295:Inositol Phosphates / Q000378:metabolism*; D008974:Mollusca; D011494:Protein Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018019:Receptors, Corticotropin-Releasing Hormone / Q000378:metabolism; D015290:Second Messenger Systems; D015398:Signal Transduction / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology; D020960:omega-Conotoxins / Q000494:pharmacology
D015342:DNA Probes; D007295:Inositol Phosphates; D012333:RNA, Messenger; D018019:Receptors, Corticotropin-Releasing Hormone; D020960:omega-Conotoxins; D003346:Corticotropin-Releasing Hormone; D014700:Verapamil; D000242:Cyclic AMP; D011494:Protein Kinases