A sequence similar to bacterial transposable IS elements present in the 5' untranslated region of the bovine butanediol dehydrogenase cDNA.
A sequence similar to prokaryotic transposable elements was identified in the long 5' untranslated region (5'UTR) of the butanediol dehydrogenase cDNA isolated from a bovine brain lambda gt11 library. Several observations suggested that this sequence could be related to bacterial IS elements: (a) 58% nucleotide sequence identity, (b) 56% amino acid sequence identity, and (c) the presence of inverted terminal repeats. However, nucleotide sequence analyses of the 5'UTR bovine cDNA showed the presence of chain-terminating nucleotide substitutions that would render it incapable of encoding a functional transposase. Finally, it was observed that different vertebrate genomes have sequences related to this putative transposable element.
Smania
A M
AM
Centro de Investigaciones en Química Biológica de Córdoba-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. asmania@dqbfcq.uncor.edu
Saleh
M C
MC
Argaraña
C E
CE
D020121:5' Untranslated Regions / Q000235:genetics*; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002417:Cattle; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D020121:5' Untranslated Regions; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase