[Comparison between the standard MPN method recommended by the American Public Health Association and the Simplate and Petrifilm methods for identification of the coliform group and Escherichia coli in tilapia (Oreochromis sp) originating from aquaculture in fresh water].
We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapia's external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1%, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100% of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.
Muratori
M C
MC
Escola de Veterinária, Belo Horizonte, MG, Brasil. chrismuratori@zipmail.com.br
Oliveira
A de L
Ade L
Ribeiro
L P
LP
Costa
A P
AP
Fernandes
S H
SH
Leite
R C
RC
D000818:Animals; D017756:Aquaculture; D015169:Colony Count, Microbial / Q000379:methods / Q000662:veterinary*; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000302:isolation & purification*; D005121:Extremities / Q000382:microbiology; D005393:Fish Diseases / Q000382:microbiology*; D005618:Fresh Water; D005880:Gills / Q000382:microbiology; D012867:Skin / Q000382:microbiology; D017210:Tilapia / Q000382:microbiology*