Molecular characterization of feline interleukin 16: chemotactic activity and effect on feline immunodeficiency virus infection and/or replication.
Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through inhibition of HIV and SIV mRNA transcription. To evaluate its role in the FIV cat model, we cloned and expressed feline IL-16 and determined its ability to induce chemotaxis as well as to inhibit FIV replication in cultured PBMCs. Sequence comparison of rfIL-16 with human, African green monkey, rhesus macaque, and mouse IL-16 showed 84.2, 84.5, 84.4, and 79.4% identity at the nucleotide sequence level and 93, 91.5, 90.7, and 87.2% identity at the amino acid sequence level, respectively. Biocharacterization of rfIL-16 revealed potent induction of chemotaxis (p < 0.05). In addition, p24 production from feline PBMCs infected with FIV Zurich 2 in vitro was decreased up to 87% (p < 0.05). These data demonstrate biologic and antiviral functionality of rfIL-16.
Leutenegger
C M
CM
Department of Internal Veterinary Medicine, University of Zurich, Switzerland. cmleutenegger@ucdavis.edu
Huder
J B
JB
Mislin
C N
CN
Lahrtz
F
F
Hofmann-Lehmann
R
R
Pedersen
N C
NC
Lutz
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D016089:Immunodeficiency Virus, Feline / Q000187:drug effects*; D019410:Interleukin-16 / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology / Q000821:virology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013047:Specific Pathogen-Free Organisms; D014779:Virus Replication / Q000187:drug effects
D019410:Interleukin-16; D011994:Recombinant Proteins