Comparative study of two ribozymes and DNA-enzyme against the same RNA target.
Two ribozymes, hammerhead ribozyme and hairpin ribozyme, and a DNA-enzyme were designed to cleave a same RNA target, the same site of the rat complement regulatory factor 512 antigen mRNA. The kinetic properties of these RNA-cleaving enzymes were measured and compared under the same conditions, using multiple turnover kinetics and competition kinetics. The catalytic efficiencies of these enzymes, and also the order of these enzymes will be discussed.
Inui
O
O
Department of Ecological Engineering, Toyohashi University of Technology, Aichi, Japan.
Kikuchi
Y
Y
Dohi
N
N
Okada
N
N
Okada
H
H
Tanaka
T
T
D000818:Animals; D000954:Antigens, Surface; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface; D011951:Receptors, Complement / Q000235:genetics; D014158:Transcription, Genetic; D014764:Viral Proteins
D000954:Antigens, Surface; C513105:Cr1l protein, rat; D016337:RNA, Catalytic; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011951:Receptors, Complement; D014764:Viral Proteins; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases