Molecular cloning of a homologue of dad-1 gene in citrus: distinctive expression during fruit development.
A cDNA homologue to the human defender against apoptotic death gene (dad-1), which is involved in programmed cell death, was isolated from satsuma mandarin (Citrus unshiu Marc.) fruit. It (Citdad-1-1) was 345 bp long, with a deduced protein sequence of 115 amino acids. Southern hybridization suggests that dad-1-related sequences are present as a small gene family in the citrus genome. Expression of Citdad-1-1 was progressively down-regulated in leaves as they matured, but not in juice sac/segment epidermis (edible part) towards fruit ripening. The role of dad-1 during citrus development is also discussed.
Moriguchi
T
T
Department of Citriculture, National Institute of Fruit Tree Science, Shimizu, Shizuoka, Japan. takaya@fruit.affrc.go.jp
Komatsu
A
A
Kita
M
M
Akimitsu
K
K
Endo-Inagaki
T
T
Omura
M
M
D000595:Amino Acid Sequence; D002957:Citrus / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016415:Sequence Alignment