title: Photodynamic antisense regulation using psoralen-conjugated oligo(nucleoside phosphorothioate)s (I). Growth regulation of cervical carcinoma cells.
abstract: To increase the antisense regulatory effect of oligo(nucleoside phosphorothioate)s (S-Oligo), a photo-crosslinking reagent, 4, 5', 8-trimethylpsoralen, was used in this study. Psoralen-conjugated oligo(nucleoside phosphorothioate) (Ps-S-Oligo) complementary to the human papillomavirus type 18 (HPV18) mRNA drastically inhibited the cellular proliferation of cervical cancer cells only upon UVA-irradiation. In contrast, Ps-S-Oligos with mismatched sequences and scrambled one showed lesser inhibitory effects than that with matched one. These results suggest that psoralen-conjugated antisense S-Oligo has significant potential to regulate gene expression upon UVA-irradiation.
authors.affiliation: Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Iwase
authors.forename: A
authors.initials: A
authors.lastname: Yamayoshi
authors.forename: J
authors.initials: J
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
authors.forename: N
authors.initials: N
authors.lastname: Wake
authors.forename: A
authors.initials: A
authors.lastname: Murakami
mesh_terms: D002455:Cell Division / Q000187:drug effects* / Q000528:radiation effects; D005260:Female; D011564:Furocoumarins / Q000633:toxicity*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*; D027383:Papillomaviridae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013873:Thionucleotides; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms
chemical_list: D011564:Furocoumarins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; D013873:Thionucleotides