A probe for the versatile analysis and characterization of N-linked oligosaccharides.
A novel fluorescent probe, 3-(acetylamino)-6-aminoacridine (AA-Ac), has been synthesized and its applicability to the analysis of picomole levels of N-linked glycans investigated. AA-Ac was found to be an excellent derivatization reagent for N-linked glycans, giving at least twice the intensity of fluorescence as its predecessor 2-aminoacridone. AA-Ac-labeled glycans were analyzed by both normal and reversed-phase HPLC. They were also amenable to enzymatic sequencing and analysis by MALDI-TOF mass spectrometry, free zone capillary electrophoresis, and capillary electrophoresis/electrospray ionization mass spectrometry.
Charlwood
J
J
New Frontiers Science Park, SmithKline Beecham Pharmaceuticals, Harlow, Essex, U.K.
Birrell
H
H
Gribble
A
A
Burdes
V
V
Tolson
D
D
Camilleri
P
P
D002240:Carbohydrate Sequence; D015335:Molecular Probes; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015335:Molecular Probes; D009844:Oligosaccharides