title: Overexpression of p53 and bcl-2 proteins and the presence of HPV infection are independent events in head and neck cancer.
abstract: The expression of p53 and bcl-2 proteins by immunohistochemistry and the identification of human papillomavirus (HPV) infection by a non-isotopic polymerase chain reaction (PCR)based method were investigated in 30 patients with head and neck cancer. Ten cases were HPV-positive (33%), mostly as double or multiple infections by high- or intermediate-risk types. Twenty-one patients were p53-positive (70%), 9/10 with HPV-positive tumours and 12/20 with HPV-negative tumours; this difference was not statistically significant. Only four cases were bcl-2-positive, irrespective of the presence of either HPV or p53. No correlation was found between these biological factors and tumour stage, differentiation grade, and alcohol or tobacco use. Our findings indicate that p53 is involved in the majority of cases, bcl-2 is rare, and high-risk HPV could play a key role, especially in tumours of tongue and tonsil. In conclusion p53 and bcl-2 protein expression and the presence of HPV infection are independent events in these malignancies.
authors.affiliation: Laboratory of Virology, Regina Elena Cancer Institute, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Badaracco
authors.forename: A
authors.initials: A
authors.lastname: Venuti
authors.forename: A
authors.initials: A
authors.lastname: Bartolazzi
authors.forename: R
authors.initials: R
authors.lastname: Morello
authors.forename: F
authors.initials: F
authors.lastname: Marzetti
authors.forename: M L
authors.initials: ML
authors.lastname: Marcante
mesh_terms: D000328:Adult; D000368:Aged; D000428:Alcohol Drinking; D000704:Analysis of Variance; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology* / Q000821:virology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology* / Q000821:virology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology; D014067:Tonsillar Neoplasms / Q000235:genetics / Q000473:pathology / Q000821:virology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014412:Tumor Virus Infections / Q000150:complications*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53