title: [Stabilization of enzymes by anabiosis autoinducers as a possible mechanism of resistance of resting microbial forms].
abstract: Alkyl-substituted hydroxybenzenes (AHBs), auto-inducers of microbial dormancy (or d1 factors), were found to stabilize the structure of protein macromolecules, making them metabolically less active and more resistant to stresses. In vitro experiments with the Bacillus intermedius ribonuclease and chymotrypsin showed that the degree of the physical and chemical stability of these enzymes treated with AHBs depends on their concentration and incubation time. Experiments with RNase, which is capable of refolding, i.e., renaturation after heat denaturation, revealed that AHBs efficiently interact with both intact and denatured proteins. The data obtained allow the inference to be made that d1 factors may play the role of natural chemical chaperons, blocking metabolism in dormant cells through the formation of catalytically inactive thermostable complexes with enzymes.
authors.affiliation: Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A I
authors.initials: AI
authors.lastname: Kolpakov
authors.forename: O N
authors.initials: ON
authors.lastname: Il'inskaia
authors.forename: M M
authors.initials: MM
authors.lastname: Bespalov
authors.forename: F G
authors.initials: FG
authors.lastname: Kupriianova-Ashina
authors.forename: V F
authors.initials: VF
authors.lastname: Gal'chenko
authors.forename: B I
authors.initials: BI
authors.lastname: Kurganov
authors.forename: G I
authors.initials: GI
authors.lastname: El'-Registan
mesh_terms: D000220:Adaptation, Biological; D001407:Bacillus / Q000502:physiology*; D002918:Chymotrypsin / Q000502:physiology*; D004795:Enzyme Stability / Q000502:physiology; D019800:Phenol / Q000378:metabolism*; D012260:Ribonucleases / Q000502:physiology*; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: D019800:Phenol; D012260:Ribonucleases; D002918:Chymotrypsin