Screening and characterization of Bacillus thuringiensis isolates from Brazil for the presence of coleoptera-specific cry genes.
Forty-three Bacillus thuringiensis isolates from Brazil and 3 from Argentina were screened, using the polymerase chain reaction (PCR), for various coleoptera-specific cry genes. Seven isolates produced specific and/or nonspecific DNA fragments in a PCR reaction with primers specific for two coleopteran cry genes and 4 of these produced DNA fragments with primers specific for 7 known coleopteran cry genes. These isolates showed, by electron microscopy, the presence of spherical crystals. They also showed proteins of around 70 kDa which were immunologically similar to the Cry3Aa protein from B. thuringiensis subsp. tenebrionis. The 3 isolates from Argentina were toxic to T. molitor, and although no isolate from Brazil showed toxicity, they might show toxicity to another insect species.
Marquez
A M
AM
Departmento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brazil.
Dias
J M
JM
Ribeiro
B M
BM
D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001427:Bacterial Toxins; D001938:Brazil; D001517:Coleoptera / Q000502:physiology*; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006460:Hemolysin Proteins; D008854:Microscopy, Electron; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction / Q000379:methods
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis