Comparison of growth rates of Tritrichomonas foetus isolates from various geographic regions using three different culture media.
The growth rates of 16 isolates of Tritrichomonas foetus from three distinct geographic regions were investigated in modified Diamond's medium, liver infusion broth medium and a commercially available culture kit. While some differences in growth characteristics were detected for different isolates and in the three different media, all isolates grew. Trichomonads reached peak concentrations from an initial concentration of 10(4) trichomonads/ml on Days 2, 3 and 4 in modified Diamond's medium, on Days 2-6 (excluding CAPTF102) in the commercial culture kit and on Days 2-7 in liver infusion broth medium. Viable parasites were detectable for longer periods in liver infusion broth medium and the commercial culture kit than in Diamond's medium. Peak concentrations for isolates tended to be higher in modified Diamond's medium than in liver infusion broth medium or the commercial culture kit. Results show that these three media are suitable for the growth of all 16 T. foetus isolates from three continents and suggest that these media could be used effectively throughout the world.
Lun
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Centre for Animal Parasitology, Canadian Food Inspection Agency, 116 Veterinary Road, Saskatoon, Canada.
Parker
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Gajadhar
A A
AA
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000469:parasitology*; D003470:Culture Media; D005260:Female; D008297:Male; D010274:Parasitology / Q000379:methods; D011528:Protozoan Infections / Q000175:diagnosis / Q000469:parasitology; D011529:Protozoan Infections, Animal; D016835:Tritrichomonas foetus / Q000254:growth & development* / Q000302:isolation & purification
D003470:Culture Media