In situ monitoring of peptide-bound dsDNA selection on a GCN4-bZIP-immobilized quartz-crystal microbalance.
A highly sensitive 27 MHz quartz-crystal microbalance (QCM) was applied as a device of dsDNA in vitro selection. When GCN4-bZIP peptides were immobilized on the small Au electrode of the QCM, dsDNAs having TGACTCA sequences could be mainly selected from DNA library having a 15-bp random region, which was consistent with the sequence observed by X-ray crystallography.
Watanabe
A
A
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Furusawa
H
H
Okahata
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D016350:Leucine Zippers; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000737:chemistry* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; D011494:Protein Kinases