Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis.
Plants are sessile organisms, and their ability to adapt to stress is crucial for survival in natural environments. Many observations suggest a relationship between stress tolerance and heat shock proteins (HSPs) in plants, but the roles of individual HSPs are poorly characterized. We report that transgenic Arabidopsis plants expressing less than usual amounts of HSP101, a result of either antisense inhibition or cosuppression, grew at normal rates but had a severely diminished capacity to acquire heat tolerance after mild conditioning pretreatments. The naturally high tolerance of germinating seeds, which express HSP101 as a result of developmental regulation, was also profoundly decreased. Conversely, plants constitutively expressing HSP101 tolerated sudden shifts to extreme temperatures better than did vector controls. We conclude that HSP101 plays a pivotal role in heat tolerance in Arabidopsis. Given the high evolutionary conservation of this protein and the fact that altering HSP101 expression had no detrimental effects on normal growth or development, one should be able to manipulate the stress tolerance of other plants by altering the expression of this protein.
Queitsch
C
C
Howard Hughes Medical Institute, Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60615-1463, USA.
Hong
S W
SW
Vierling
E
E
Lindquist
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D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D015153:Blotting, Western; D017124:Conserved Sequence / Q000235:genetics; D016373:DNA, Antisense / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D020043:Genes, Essential / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D018525:Germination / Q000235:genetics / Q000502:physiology; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D018869:Heat-Shock Response / Q000235:genetics* / Q000502:physiology*; D006358:Hot Temperature; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D016373:DNA, Antisense; C115569:HSP101 protein, plant; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D014157:Transcription Factors