Folding transition of long duplex DNA from mammalian cells.
Conformational change in individual giant DNAs from pig liver is studied by use of fluorescence microscopy. With the addition of spermidine (a trivalent amine), each DNA chains undergo abrupt transition from an elongated coiled state into a folded compact state. It is found that the all-or-none characteristics in the folding transition for the mammalian DNA is similar to that in phage DNAs.
Yoshikawa
Y
Y
Department of Food and Nutrition, Nagoya Bunri College, Japan.
Kanbe
T
T
Yoshikawa
K
K
D000818:Animals; D004247:DNA / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure; D008099:Liver / Q000737:chemistry*; D008322:Mammals; D008856:Microscopy, Fluorescence / Q000379:methods; D009690:Nucleic Acid Conformation / Q000187:drug effects; D013095:Spermidine / Q000494:pharmacology; D013552:Swine
D004247:DNA; D013095:Spermidine