title: Significance of serum type IV collagenolytic activities and gelatinase levels for detection of metastasis in murine RCT sarcoma.
abstract: We investigated the usefulness of serum type IV collagenolytic activities and gelatinase levels as diagnostic markers of metastasis in the animal model of spontaneous lung metastasis by FITC-labeled type IV collagen degradation assay and zymographic analyses. High-metastatic RCT(+) and low-metastatic RCT(-) clones were used in the present study. The mean serum type IV collagenolytic activity in the RCT(+) group started to increase from two weeks after hind limb amputation, and was 0.45 and 1.29 unit/ml at three and four weeks. These values were significantly higher than those in the control group (p < 0.01 at three weeks; p < 0.001 at four weeks). A correlation between the number of lung nodules and serum type IV collagenolytic activities in the RCT(+) group was found (r = 0.89, p < 0.001). Zymographic analyses indicated that 105-kD gelatinolytic activities of the RCT(+) group were higher than those of the RCT(-) group at three and four weeks. Thus, type IV collagenolytic activities and serum gelatinase levels might be valuable markers for the detection of metastasis.
authors.affiliation: Department of Orthopaedic Surgery, Toyama Medical and Pharmaceutical University, Sugitani, Japan. ohmori@ms.toyama-mpu.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Ohmori
authors.forename: M
authors.initials: M
authors.lastname: Kanamori
authors.forename: K
authors.initials: K
authors.lastname: Yudoh
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D003094:Collagen / Q000097:blood*; D003937:Diagnosis, Differential; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D018093:Gelatinases / Q000097:blood*; D006868:Hydrolysis; D008175:Lung Neoplasms / Q000201:enzymology* / Q000556:secondary; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012513:Sarcoma, Experimental / Q000201:enzymology* / Q000556:secondary; D012878:Skin Neoplasms / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D003094:Collagen; D018093:Gelatinases; D016650:Fluorescein-5-isothiocyanate