[Isolation and study of recombinant strains of Salmonella typhimurium SL 7207, producing HBcAG and HBcAG-HBs].
Recombinant strains producing hepatitis B virus (HBcAg) core protein and chimeric core protein exposing on its surface the major immunogenic epitope of HBsAg (HBcAg-HBs) were constructed on the base of attenuated S. typhimurium SL 7202 strain. The resultant Salmonella strains produced proteins which were capable of self-assembly into virus-like particles and showed antigenic properties of both core and surface hepatitis B proteins. A single rectal immunization with recombinant S. typhimurium induced humoral and cellular immune response to HBcAg and HBsAg. Specific anti-HBcAg were detected in animal sera and intestinal tissues, which indicated the formation of specific mucosal immunity.
Karpenko
L I
LI
Ignat'ev
G M
GM
Kozhina
E M
EM
Kashentseva
E A
EA
Poryvaeva
V A
VA
Melamed
N V
NV
Baĭborodin
S I
SI
Smirnova
O Iu
OIu
Il'ichev
A A
AA
D000818:Animals; D000939:Epitopes / Q000276:immunology; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000235:genetics*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology
D000939:Epitopes; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens