Vaccination against coccidiosis: host strain-dependent evocation of protective and suppressive subsets of murine lymphocytes.
BALB/c mice are normally more resistant than C57BL/6 (B6) mice to infection with Eimeria vermiformis, but these phenotypes can be reversed by oral or parenteral vaccination with a crude antigen prepared from the parasite. Treatment of mice with antibodies specific for CD4+ or CD8+ T cells showed that the increased susceptibility of vaccinated BALB/c mice was associated with the presence of CD4+ T cells. This finding was confirmed when the recipients of CD4+ T cells selected from the mesenteric lymph nodes (MLN) of vaccinated BALB/c mice produced more oocysts after challenge than the recipients of a similar population of cells from sham-vaccinated mice. The residual population of cells (presumably enriched for CD8+ T cells, 'CD8+'), on the other hand, conferred some protection and, in B6 mice, the findings were reversed. Thus, vaccination induced suppressive or protective CD4+ cells and protective or suppressive 'CD8+' cells, depending upon the normal resistance/susceptibility phenotype of the host. Examinations of the isotypes (IgG1, IgG2a) of specific serum antibodies, and of the levels of IFN-gamma and IL-5 cytokines released by MLN cells stimulated ex vivo, did not allow any further characterization of the mechanisms involved.
Rose
M E
ME
Division of Immunology and Pathology, Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, UK.
Hesketh
P
P
Grencis
R K
RK
Bancroft
A J
AJ
D019264:Adoptive Transfer; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000008:administration & dosage; D000961:Antilymphocyte Serum / Q000008:administration & dosage; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D003048:Coccidiosis / Q000276:immunology* / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D004539:Eimeria / Q000276:immunology; D005260:Female; D007132:Immunoglobulin Isotypes / Q000097:blood; D066298:In Vitro Techniques; D008212:Lymphocyte Depletion; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D014611:Vaccination
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000961:Antilymphocyte Serum; D016207:Cytokines; D007132:Immunoglobulin Isotypes