Synthesis of oligonucleotides containing the oxidatively modified kappaB site: unique binding affinity to the NFkappaB p50 homodimer.
Synthesis of oligonucleotide 26-mers including single 5-formyl-2'-deoxyuridine (1) or 5-formyl-2'-O-methyluridine (2) in place of thymidine at the kappaB site has been accomplished. One of the 26-mers with 1 was critically discriminated by the NFkappaB p50 homodimer in binding.
Kittaka
A
A
Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, Japan.
Takayama
H
H
Horii
C
C
Kuze
T
T
Tanaka
H
H
Nakamura
K T
KT
Miyasaka
T
T
Inoue
J
J
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry; D019281:Dimerization; D016328:NF-kappa B / Q000737:chemistry* / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D010084:Oxidation-Reduction
D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D004247:DNA