title: Short report: possible Cryptosporidium muris infection in humans.
abstract: Oocysts of cryptosporidia whose morphology resembled that of Cryptosporidium muris were found in the stool of 2 healthy girls in Surabaya, Indonesia. The oocysts were predominantly oval and measured 7.75+/-0.17 x 5.55+/-0.13 microm (mean+/-SD). The number of oocysts excreted were more than 10(5) per gram of stool. The oocysts were well stained with fluorescein-conjugated monoclonal antibody to Cryptosporidium. The specimens from both girls containing the oocysts showed a positive result by the polymerase chain reaction (PCR) using primers specific for the genus Cryptosporidium, but a negative result by the PCR using primers specific for C. parvum. The 2 girls passed oocysts for 5 and 6 days, respectively. They did not complain of any symptoms during the passage of oocysts.
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Katsumata
authors.forename: D
authors.initials: D
authors.lastname: Hosea
authors.forename: I G
authors.initials: IG
authors.lastname: Ranuh
authors.forename: S
authors.initials: S
authors.lastname: Uga
authors.forename: T
authors.initials: T
authors.lastname: Yanagi
authors.forename: S
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000469:parasitology* / Q000473:pathology; D003458:Cryptosporidium / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007214:Indonesia; D008858:Microscopy, Phase-Contrast; D016133:Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D016054:DNA, Protozoan