title: Heat-induced conformational transition of cytochrome c observed by temperature gradient gel electrophoresis at acidic pH.
abstract: Temperature-gradient gel electrophoresis (TGGE) has been used to study the thermal unfolding of ferricytochrome c in low and high concentrations of acetic acid. It has been observed that the mobility of cytochrome c is a linear function of temperature when the system is characterized by a homogeneous population of conformation-state, single molecular species. Within the transition temperature range, the mobility clearly displays the characteristic sigmoidal shape describing the transitions of protein unfolding. The data obtained by TGGE were used to estimate the apparent thermodynamic parameters (enthalpy change deltaHvh and transition temperature Tm), associated with the transition of unfolding. The accuracy of the apparent thermodynamic parameters obtained by this method agrees within error limits with the values obtained by direct calorimetric measurements using differential scanning calorimetry (DSC).
authors.affiliation: P.J. Safárik University, Faculty of Sciences, Department of Biochemistry, Kosice, Slovakia.
authors.forename: V
authors.initials: V
authors.lastname: Víglaský
authors.forename: M
authors.initials: M
authors.lastname: Antalík
authors.forename: J
authors.initials: J
authors.lastname: Bagel'ová
authors.forename: Z
authors.initials: Z
authors.lastname: Tomori
authors.forename: D
authors.initials: D
authors.lastname: Podhradský
mesh_terms: D002152:Calorimetry, Differential Scanning; D003574:Cytochrome c Group / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D003574:Cytochrome c Group