Short report: possible Cryptosporidium muris infection in humans.
Oocysts of cryptosporidia whose morphology resembled that of Cryptosporidium muris were found in the stool of 2 healthy girls in Surabaya, Indonesia. The oocysts were predominantly oval and measured 7.75+/-0.17 x 5.55+/-0.13 microm (mean+/-SD). The number of oocysts excreted were more than 10(5) per gram of stool. The oocysts were well stained with fluorescein-conjugated monoclonal antibody to Cryptosporidium. The specimens from both girls containing the oocysts showed a positive result by the polymerase chain reaction (PCR) using primers specific for the genus Cryptosporidium, but a negative result by the PCR using primers specific for C. parvum. The 2 girls passed oocysts for 5 and 6 days, respectively. They did not complain of any symptoms during the passage of oocysts.
Katsumata
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T
Department of Parasitology, Institute of Tropical Medicine, Nagasaki University, Japan.
Hosea
D
D
Ranuh
I G
IG
Uga
S
S
Yanagi
T
T
Kohno
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000469:parasitology* / Q000473:pathology; D003458:Cryptosporidium / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007214:Indonesia; D008858:Microscopy, Phase-Contrast; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D016054:DNA, Protozoan