Upregulation of bradykinin B1-receptor expression after myocardial infarction.
To determine the influence of the myocardial infarction (MI) on bradykinin B1-receptor (B1R) regulation, we studied its expression in the left ventricle (LV) after MI. Rats were submitted to a permanent occlusion of the left coronary artery. Six hours, 24 h and 6 days after MI or after sham operation, left ventricular pressure (LVP) and dP/dt(max) were measured. LV-total RNA was extracted and B1R expression was analysed by a RNase-protection assay (each group n = 6). LVP and dP/dt(max) were impaired at all time points after MI. Basal B1R expression was not detectable in controls. Six hours after MI, the B1R expression was upregulated and reached a maximum 24 h after MI (4 fold vs. 6 h). Six days post-MI, B1R expression returned to levels found 6 h after MI. These data are the first demonstration for an induced myocardial B1R expression in an in vivo model of MI.
Tschöpe
C
C
Department of Cardiology and Pneumology, University Hospital Benjamin Franklin, Free University of Berlin, Germany. tsch@zedat.fu-Berlin.de
Heringer-Walther
S
S
Koch
M
M
Spillmann
F
F
Wendorf
M
M
Leitner
E
E
Schultheiss
H P
HP
Walther
T
T
D000818:Animals; D004195:Disease Models, Animal; D006439:Hemodynamics; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin / Q000096:biosynthesis* / Q000378:metabolism; D015854:Up-Regulation; D016277:Ventricular Function, Left
D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin