Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells.
A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in all of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.
Zhang
R G
RG
Shanghai Institute of Cell Biology, Chinese Academy of Sciences.
Wang
X W
XW
Yuan
J H
JH
Guo
L X
LX
Xie
H
H
D000818:Animals; D006801:Humans; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000737:chemistry* / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000737:chemistry* / Q000201:enzymology*; D021141:Nucleic Acid Amplification Techniques; D011868:Radioisotopes; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019098:Telomerase / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D011868:Radioisotopes; D019098:Telomerase