title: Transcriptional and post-transcriptional in organello labelling of Trypanosoma brucei mitochondrial RNA.
abstract: In organello labelling of Trypanosoma brucei mitochondrial (mt) RNA was characterised with respect to nucleotide requirements and drug sensitivity. Mitochondrial transcriptional activity is maximal in the presence of all ribonucleoside-triphosphate NTPs, and can be inhibited by UTP depletion. Mitochondrial transcription can also be partially inhibited by actinomycin D (actD) or ethidium bromide (EtBr). Post-transcriptional UTP incorporation is insensitive to actinomycin D or ethidium bromide. Proteins were identified that interact with transcriptional and post-transcriptionally labelled RNAs, and confirm the in vitro RNA-binding properties discovered for a number of T. brucei mt proteins. These experiments reveal new strategies for studying mt transcription and processing in T. brucei mitochondria.
authors.affiliation: Department of Microbiology and Center for Microbial Pathogenesis, SUNY at Buffalo School of Medicine, Buffalo, NY 14214, USA.
authors.forename: K T
authors.initials: KT
authors.lastname: Militello
authors.forename: M L
authors.initials: ML
authors.lastname: Hayman
authors.forename: L K
authors.initials: LK
authors.lastname: Read
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D012313:RNA / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D000077278:RNA, Mitochondrial; D012313:RNA