Subgroup determination of respiratory syncytial virus by RT-PCR.
PCR optimization for differentiation of RSV subgroup A (RT-PCR-1) and RSV subgroup B (RT-PCR-2) were developed. Various conditions of RT-PCR-1 and RT-PCR-2 were summarized. These methods are highly specific and sensitive to differentiate RSV subgroup A and RSV subgroup B from the other respiratory viruses.
Tantivanich
S
S
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Suphanaranonda
K
K
Balachanda
K
K
D002675:Child, Preschool; D004812:Epidemiologic Methods; D006801:Humans; D007223:Infant; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000145:classification* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013785:Thailand / Q000453:epidemiology