Transalkylation of phosphotriesters using Cob(I)alamin: toward specific determination of DNA-phosphate adducts.
The supernucleophilic cobalt compound, cob(I)alamin, has been kinetically characterized with respect to its ability to bring about transalkylation of adducts to DNA phosphates (phosphotriesters). The reactivity of cob(I)alamin toward different phosphotriesters (model compounds and methylated DNA), as well as its specificity toward DNA-phosphate adducts, has been investigated. Through nucleophilic displacement on the alkyl by cob(I)alamin, the alkyl groups (methyl and ethyl) were transferred from phosphotriesters within minutes at room temperature. In contrast, methylated nucleosides (base adducts) were stable in the presence of cob(I)alamin.
Haglund
J
J
Departments of Genetic and Cellular Toxicology and of Environmental Chemistry, Stockholm University, S-106 91 Stockholm, Sweden.
Rafiq
A
A
Ehrenberg
L
L
Golding
B T
BT
Törnqvist
M
M
D000478:Alkylation; D018736:DNA Adducts / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014805:Vitamin B 12 / Q000378:metabolism*
D018736:DNA Adducts; D014805:Vitamin B 12