[Methods of automated cell analysis and their application in radiation biology].
The present review is concerned with the methods of automated analysis of biological micro-objects and covers two groups into which all the systems of automated analysis can be divided--systems of flow (flow cytometry) and scanning (image analysis systems) type. Particular emphasis has been placed on their use in radiobiological studies, namely, in the micronucleus test, a cytogenetic assay for monitoring the clastogenic action of ionizing radiation commonly used at present. It is evident that the problem is acute, with of radiobiologists' interest in the biological action of low-dose radiation recently increasing. In addition, the estimation of a low-level damage requires the analysis of a large number of experimental objects. Examples of using both the methods elsewhere and actual setups are given. The analysis of advantages and disadvantages of the methods of automated cell analysis enables us to choose more thoroughly between the systems of flow and scanning type to use them in particular research.
Klokov
D Iu
DIu
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Oblast, Russia.
Zaichkina
S I
SI
D000818:Animals; D001330:Electronic Data Processing; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007091:Image Processing, Computer-Assisted; D011853:Radiobiology / Q000295:instrumentation / Q000379:methods*