title: Biodegradation of diethyl phthalate in soil by a novel pathway.
abstract: Biodegradation of diethyl phthalate (DEP) has been shown to occur as a series of sequential steps common to the degradation of all phthalates. Primary degradation of DEP to phthalic acid (PA) has been reported to involve the hydrolysis of each of the two diethyl chains of the phthalate to produce the monoester monoethyl phthalate (MEP) and then PA. However, in soil co-contaminated with DEP and MeOH, biodegradation of the phthalate to PA resulted in the formation of three compounds, in addition to MEP. These were characterised by gas chromatography-electron ionisation mass spectrometry and nuclear magnetic resonance as ethyl methyl phthalate, dimethyl phthalate and monomethyl phthalate, and indicated the existence of an alternative pathway for the degradation of DEP in soil co-contaminated with MeOH. Transesterification or demethylation were proposed as the mechanisms for the formation of the three compounds, although the 7:1 ratio of H(2)O to MeOH means that transesterification is unlikely.
authors.affiliation: Department of Biosciences, Research School of Biosciences, University of Kent, Canterbury, UK.
authors.forename: C D
authors.initials: CD
authors.lastname: Cartwright
authors.forename: S A
authors.initials: SA
authors.lastname: Owen
authors.forename: I P
authors.initials: IP
authors.lastname: Thompson
authors.forename: R G
authors.initials: RG
authors.lastname: Burns
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004951:Esterification; D008401:Gas Chromatography-Mass Spectrometry; D000432:Methanol / Q000378:metabolism; D008745:Methylation; D010795:Phthalic Acids / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D010795:Phthalic Acids; D012989:Soil Pollutants; C007379:diethyl phthalate; D000432:Methanol