The functional analysis of 4.5S RNA in ribosomal translocation.
4.5S RNA is a stable 114-nucleotide RNA of the bacterium Escherichia coli (E. coli). We found that 4.5S RNA have the ability of binding EF-G using gel mobility shift assay. Increasing in the concentration of GDP increase the binding activity of 4.5S RNA to EF-G. Based on these data, we propose that 4.5S RNA release EF-G from ribosome.
Ishibashi
M
M
Institute of Biological Science, University of Tsukuba, Tsukuba Science City, Japan.
Nakamura
K
K
Yamane
K
K
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D020653:Peptide Elongation Factor G / Q000378:metabolism*; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
C409968:4.5S RNA; D020653:Peptide Elongation Factor G; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; C088179:RNA, ribosomal, 45S