title: Sonication of chitin-glucan, preparation of water-soluble fractions and characterization by HPLC.
abstract: A water-insoluble chitin-glucan complex, isolated from the mycelium of Aspergillus niger, was swollen in various aqueous media and treated subsequently by high-energy sonication. The concentration of the resulting water-soluble polysaccharide fractions was dependent on the swelling medium, the amount of the chitin-glucan complex in the suspension, and on the time of sonication. The yields of water-soluble chitin-glucan were within the range 13.6 to 24.4% relative to the mass of the original chitin-glucan. The nitrogen content obtained for the samples of water-soluble chitin-glucan indicated a higher content of chitin (3.45% of nitrogen in high-molecular fraction) than in the original water-insoluble chitin-glucan sample (1.8%). The distribution of the molecular weights of the water-soluble fractions prepared was determined by high-performance liquid chromatography.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic. mislovicova@savba.sk
authors.forename: D
authors.initials: D
authors.lastname: Mislovicová
authors.forename: J
authors.initials: J
authors.lastname: Masárová
authors.forename: K
authors.initials: K
authors.lastname: Bendzálová
authors.forename: L
authors.initials: L
authors.lastname: Soltés
authors.forename: E
authors.initials: E
authors.lastname: Machová
mesh_terms: D001234:Aspergillus niger / Q000737:chemistry; D002686:Chitin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005936:Glucans / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D014462:Ultrafiltration; D014465:Ultrasonics
chemical_list: D005936:Glucans; D002686:Chitin