Conus peptides: novel probes for nicotinic acetylcholine receptor structure and function.
Conus is a genus of predatory marine snails that uses venom to capture prey. Among the neurotoxins widely utilized by the cone snails are the alpha-conotoxins which are disulfide-rich peptides that target muscle or neuronal subtypes of nicotinic acetylcholine receptors. The small size and receptor subtype specificity of these peptides make them particularly useful for characterizing both native and heterologously expressed nicotinic receptors. In this report, we demonstrate that alpha-conotoxin MII potently blocks beta3-containing neuronal nicotinic receptors. Furthermore, initial evidence suggests that subpopulations of alpha3beta2beta3-containing receptors are differentially sensitive to alpha-conotoxin MII. Thus, alpha-conotoxin MII promises to be a useful tool for studying neuronal nicotinic receptors containing the beta3 subunit.
McIntosh
J M
JM
Department of Psychiatry, University of Utah, Salt Lake City, UT 84112, USA. mcintosh@bioscience.utah.edu
Gardner
S
S
Luo
S
S
Garrett
J E
JE
Yoshikami
D
D
D000818:Animals; D020916:Conotoxins / Q000494:pharmacology*; D004594:Electrophysiology; D006801:Humans; D015335:Molecular Probes; D018733:Nicotinic Antagonists / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000187:drug effects; D012908:Snails; D014981:Xenopus
D020916:Conotoxins; D015335:Molecular Probes; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D011993:Recombinant Fusion Proteins; C098872:alpha-conotoxin MII