[Growth of human respiratory epithelium on collagen foil].
BACKGROUND
Clinical application of bioartificial tracheal prosthesis must still be regarded as an experimental concept because restoration of a functional respiratory epithelium outlining the prosthesis is still not possible. Tissue engineering as a relatively new biotechnological discipline may offer new methods in expanding differentiated respiratory epithelium in vitro. In this study we compare two different cell and tissue culture procedures for growing human nasal mucosa on commercially available collagen foil.


METHODS
Harvested specimens of human nasal mucosa (n = 6, 4 x 4 cm) were placed on collagen foil and incubated as tissue cultures for 4, 6 and 8 weeks. A suspension of enzymatically dispersed nasal epithelium seeded on collagen foil (5 x 10(5) cells) served as control. Cell growth and ciliary beat were monitored through an inverted microscope with Hoffman's modulation contrast and video set-up. Histological examination was performed after 4, 6 and 8 weeks.


RESULTS
In the tissue cultures, the collagen foil was initially covered with fibroblasts growing from the mucosa specimen before epithelial cells spread out. The epithelial layer showed mostly ciliated cells which developed metachronous ciliary beat after 4 weeks in vitro. Ciliary activity was observed until the end of the experiments in 8 weeks. New cells on the suspension cultures were mesenchymal and did not exhibit any ciliary activity.


CONCLUSIONS
Mucosa specimens seem to be more appropriate for tissue engineering of respiratory epithelium than cell suspensions from nasal epithelium. Collagen foil as tissue scaffold initiates epithelial-mesenchymal interaction and may play an important role in epithelial differentiation of new respiratory epithelium.
Bücheler
M
M
Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Plastische Operationen, Universität Leipzig.
Scheffler
B
B
von Foerster
U
U
Bruinink
A
A
Bootz
F
F
Wintermantel
E
E
D001698:Biomedical Engineering; D001709:Biotechnology; D018929:Cell Culture Techniques; D002923:Cilia / Q000502:physiology; D003094:Collagen; D046508:Culture Techniques; D004847:Epithelial Cells / Q000166:cytology*; D005347:Fibroblasts; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology / Q000254:growth & development*; D019736:Prostheses and Implants; D020545:Respiratory Mucosa / Q000166:cytology / Q000254:growth & development; D013535:Suspensions; D013997:Time Factors; D014132:Trachea
D013535:Suspensions; D003094:Collagen