Mechanism of ribosome RNA apurinic site specific lyase.
A new enzyme, which we named ribosome RNA apurinic site specific lyase (RALyase), has been characterized. The enzyme specifically cleaves a phosphodiester bond at the apurinic site in the sarcin/ricin domain of 28S rRNA in ribosomes. The cut ends of wheat 28S rRNA were determined as 5'---GUACG-alpha-hydroxy-alpha, beta-unsaturated aldehyde and pGAGGA---3' for the 3' fragment, demonstrating that the enzyme catalyzes the beta-elimination reaction.
Sawasaki
T
T
Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama, Japan.
Morishita
R
R
Ozawa
A
A
Ogasawara
T
T
Madin
K
K
Endo
Y
Y
D001080:Apurinic Acid; D001483:Base Sequence; D002384:Catalysis; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins; D007700:Kinetics; D037121:Plant Lectins; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012276:Ricin / Q000235:genetics; D013379:Substrate Specificity; D014908:Triticum
D005656:Fungal Proteins; D037121:Plant Lectins; D012339:RNA, Ribosomal, 28S; D001080:Apurinic Acid; C014801:alpha-sarcin; D012276:Ricin; D004722:Endoribonucleases; C402789:ribosomal RNA apurinic site specific lyase