Expression of PGF(2alpha) receptor mRNA in normal, hyperplastic and neoplastic skin.
Reverse transcription polymerase chain reaction (RT-PCR) and Northern blot analysis was used to determine the level of expression of prostaglandin F(2alpha) (FP) receptor mRNA in various mouse tissues, including normal, hyperplastic and neoplastic mouse epidermis. Steady-state concentrations of FP receptor mRNA were low in normal and hyperplastic epidermis. The response of the epidermis to the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) was biphasic in that FP receptor mRNA was increased immediately after treatment, followed by a long-lasting down-regulation at later time points. FP receptor mRNA was down-regulated in the majority of papillomas obtained by the mouse skin carcinogenesis initiation-promotion protocol. In carcinomas, FP receptor mRNA expression was similar to that in normal epidermis. The steady-state concentration of FP mRNA was inversely correlated with PGF(2alpha) levels in normal and hyperplastic epidermis and in papillomas, indicating that FP mRNA expression is regulated by this eicosanoid.
Müller
K
K
Research Program Tumor Cell Regulation, Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany.
Krieg
P
P
Marks
F
F
Fürstenberger
G
G
D000293:Adolescent; D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology; D017931:DNA Primers; D015237:Dinoprost / Q000378:metabolism*; D005260:Female; D006801:Humans; D006965:Hyperplasia; D051379:Mice; D016133:Polymerase Chain Reaction; D011982:Receptors, Prostaglandin / Q000235:genetics* / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002273:Carcinogens; D017931:DNA Primers; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D015237:Dinoprost; D013755:Tetradecanoylphorbol Acetate