title: Bone material elasticity in a murine model of osteogenesis imperfecta.
abstract: To investigate the source of bone brittleness in the disease osteogenesis imperfecta (OI), biomechanical properties have been measured in the femurs from a homozygous (oim/oim) mutant mouse model of OI, its heterozygous littermates, and wild-type animals. The novel technique of ultrasound critical-angle reflectometry (UCR) was used to determine bone material elasticity matrix from measurements of the pressure and shear wave velocity at different orientations about selected points of the bone specimens. This nondestructive method is the only available means for obtaining measurements of this nature from a single surface. The ultrasound pressure wave velocity showed an increased isotropy in the homozygous compared to the wild-type specimens. This was reflected in a significant decrease in the principal elastic modulus measured along the length of the oim/oim bones (E33) while the modulus along the width (E11) did not change significantly, compared to wild-type specimens. The Poisson's ratio, v12, also had a significantly increased value in oim/oim bones. Measurements of these parameters in heterozygous animals generally fell between those from homozygous and control mice. The differences in the elasticity components in oim/oim bones indicate an altered stress distribution and a modified elastic response to loads, compared to normal bone.
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235-9058, USA. shreefal.mehta@email.swmed.edu
authors.forename: S S
authors.initials: SS
authors.lastname: Mehta
authors.forename: P P
authors.initials: PP
authors.lastname: Antich
authors.forename: W J
authors.initials: WJ
authors.lastname: Landis
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004548:Elasticity; D005269:Femur / Q000000981:diagnostic imaging / Q000503:physiopathology*; D051379:Mice; D010013:Osteogenesis Imperfecta / Q000000981:diagnostic imaging / Q000503:physiopathology*; D014463:Ultrasonography / Q000379:methods