title: Structure of the coat protein-binding domain of the scaffolding protein from a double-stranded DNA virus.
abstract: Scaffolding proteins are required for high fidelity assembly of most high T number dsDNA viruses such as the large bacteriophages, and the herpesvirus family. They function by transiently binding and positioning the coat protein subunits during capsid assembly. In both bacteriophage P22 and the herpesviruses the extreme scaffold C terminus is highly charged, is predicted to be an amphipathic alpha-helix, and is sufficient to bind the coat protein, suggesting a common mode of action. NMR studies show that the coat protein-binding domain of P22 scaffolding protein exhibits a helix-loop-helix motif stabilized by a hydrophobic core. One face of the motif is characterized by a high density of positive charges that could interact with the coat protein through electrostatic interactions. Results from previous studies with a truncation fragment and the observed salt sensitivity of the assembly process are explained by the NMR structure.
authors.affiliation: Comprehensive Cancer Center, Birmingham, AL, 35294, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.forename: M H
authors.initials: MH
authors.lastname: Parker
authors.forename: P
authors.initials: P
authors.lastname: Weigele
authors.forename: S
authors.initials: S
authors.lastname: Casjens
authors.forename: P E
authors.initials: PE
authors.lastname: Prevelige
authors.forename: N R
authors.initials: NR
authors.lastname: Krishna
mesh_terms: D000595:Amino Acid Sequence; D017100:Bacteriophage P22 / Q000737:chemistry* / Q000502:physiology; D002213:Capsid / Q000378:metabolism*; D018257:Helix-Loop-Helix Motifs; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D055672:Static Electricity; D014461:Ultracentrifugation; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D010446:Peptide Fragments; D015678:Viral Structural Proteins; C111088:scaffolding protein, bacteriophage P22