title: Supercomplexes in the respiratory chains of yeast and mammalian mitochondria.
abstract: Around 30-40 years after the first isolation of the five complexes of oxidative phosphorylation from mammalian mitochondria, we present data that fundamentally change the paradigm of how the yeast and mammalian system of oxidative phosphorylation is organized. The complexes are not randomly distributed within the inner mitochondrial membrane, but assemble into supramolecular structures. We show that all cytochrome c oxidase (complex IV) of Saccharomyces cerevisiae is bound to cytochrome c reductase (complex III), which exists in three forms: the free dimer, and two supercomplexes comprising an additional one or two complex IV monomers. The distribution between these forms varies with growth conditions. In mammalian mitochondria, almost all complex I is assembled into supercomplexes comprising complexes I and III and up to four copies of complex IV, which guided us to present a model for a network of respiratory chain complexes: a 'respirasome'. A fraction of total bovine ATP synthase (complex V) was isolated in dimeric form, suggesting that a dimeric state is not limited to S.cerevisiae, but also exists in mammalian mitochondria.
authors.affiliation: Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, D-60590 Frankfurt, Germany. schaegger@zbc.klinik-uni-frankfurt.de
authors.forename: H
authors.initials: H
authors.lastname: Schägger
authors.forename: K
authors.initials: K
authors.lastname: Pfeiffer
mesh_terms: D025181:ATP Synthetase Complexes; D000818:Animals; D002417:Cattle; D003902:Detergents / Q000378:metabolism; D004072:Digitonin / Q000378:metabolism; D004579:Electron Transport; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008928:Mitochondria / Q000737:chemistry*; D008929:Mitochondria, Heart / Q000737:chemistry; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000737:chemistry; D010085:Oxidative Phosphorylation; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000737:chemistry; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000201:enzymology
chemical_list: D003902:Detergents; D009097:Multienzyme Complexes; D025181:ATP Synthetase Complexes; D017856:Phosphotransferases (Phosphate Group Acceptor); D004072:Digitonin