The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation.
In Xenopus, zygotic transcription starts 6 hours after fertilization at the midblastula transition and therefore the first steps in embryonic development are regulated by maternally inherited proteins and mRNAs. While animal-vegetal polarity is already present in the oocyte, the dorsoventral axis is only established upon fertilization by the entry of the sperm and the subsequent rotation of the egg cortex. In a screen for maternal mRNAs whose stability is regulated by this cortical rotation, we isolated the Xenopus homologue of the Drosophila gene Bicaudal-C (xBic-C). It encodes a putative RNA-binding molecule expressed maternally and localized predominantly to the vegetal half of the egg. Upon fertilization and cortical rotation, xBic-C mRNA is displaced together with the heavy yolk towards the future dorsal side of the embryo. In UV-ventralized embryos, xBic-C is polyadenylated less than in untreated embryos that undergo cortical rotation. Overexpression of xBic-C by injection of synthetic mRNA in whole embryos or in ectodermal explants leads to ectopic endoderm formation. This endoderm-inducing activity is dependent on the presence of the RNA-binding domain of the protein. In contrast to the two other known maternally encoded endoderm inducers, Vg1 and VegT, xBic-C ectopic expression leads specifically to endoderm formation in the absence of mesoderm induction.
Wessely
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Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, USA.
De Robertis
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D004627:Embryonic Induction / Q000502:physiology*; D004707:Endoderm / Q000502:physiology*; D015870:Gene Expression; D019476:Insect Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000502:physiology
C453440:BICC1 protein, Xenopus; C113767:BicC protein, Drosophila; D018076:DNA, Complementary; D029721:Drosophila Proteins; D019476:Insect Proteins; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins