title: Isolation and measurement of carbonic anhydrase isoenzymes in erythrocytes of dogs.
abstract: OBJECTIVE
  To purify canine carbonic anhydrase (CA) isoenzymes CA-I and CA-II and to determine concentrations of CA-I and CA-II in erythrocytes of Beagles and dogs native to Japan.
  
  
  METHODS
  Blood samples from 116 Beagles, including 24 pregnant Beagles, and blood samples from 29 dogs native to Japan.
  
  
  METHODS
  Canine CA-I and CA-II were purified by use of column chromatography. Concentrations of CA-I and CA-II in erythrocytes of dogs were determined, using an ELISA.
  
  
  RESULTS
  Mean (+/- SD) concentrations of CA-I and CA-II in erythrocytes of Beagles were 3.21+/-0.86 and 1.63+/-0.39 mg/g of Hb, respectively. Mean concentration of CA-I was greater in male Beagles than female Beagles. In contrast, mean concentration of CA-II was greater in female Beagles than male Beagles. Furthermore, concentration of CA-II was greater in pregnant female Beagles than male or nonpregnant female Beagles. Mean concentrations of CA-I and CA-II in erythrocytes of dogs native to Japan were 11.03+/-4.39 and 3.29+/-0.91 mg/g of Hb, respectively. Mean concentration of CA-I was greater in male dogs from Japan than female dogs from Japan.
  
  
  CONCLUSIONS
  The ELISA used in this study proved to be precise and sensitive for determining CA-I and CA-II concentrations in dogs. The ELISA may enable study of changes in isoenzymes associated with hereditary or metabolic disorders of blood or other body fluids, using only a small sample. Measurement of the concentrations of CA isoenzymes in dogs may be of diagnostic value.
authors.affiliation: Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishita
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: H
authors.initials: H
authors.lastname: Ochiai
authors.forename: M
authors.initials: M
authors.lastname: Asari
mesh_terms: D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification*; D004285:Dogs / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D007525:Isoelectric Focusing / Q000662:veterinary; D007564:Japan; D008297:Male; D011247:Pregnancy
chemical_list: D002256:Carbonic Anhydrases