title: The ArcB sensor kinase of Escherichia coli: genetic exploration of the transmembrane region.
abstract: The Arc two-component signal transduction system of Escherichia coli regulates the expression of numerous operons in response to respiratory growth conditions. Cellular redox state or proton motive force (Delta(H(+))) has been proposed to be the signal for the membrane-associated ArcB sensor kinase. This study provided evidence for a short ArcB periplasmic bridge that contains a His47. The dispensability of this amino acid, the only amino acid with a pK in the physiological range, renders the Delta(H(+)) model unlikely. Furthermore, results from substituting membrane segments of ArcB with counterparts of MalF indicate that the region does not play a stereospecific role in signal reception.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: O
authors.initials: O
authors.lastname: Kwon
authors.forename: D
authors.initials: D
authors.lastname: Georgellis
authors.forename: A S
authors.initials: AS
authors.lastname: Lynch
authors.forename: D
authors.initials: D
authors.lastname: Boyd
authors.forename: E C
authors.initials: EC
authors.lastname: Lin
mesh_terms: D000469:Alkaline Phosphatase; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029968:Escherichia coli Proteins; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D018892:Proton-Motive Force / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D006639:Histidine; D011494:Protein Kinases; D018844:Cyclin-Dependent Kinases; C065271:arcB protein, E coli; D000469:Alkaline Phosphatase; C114089:phoA protein, E coli