Acute metobromuron poisoning with severe associated methemoglobinemia. Identification of four metabolites in plasma and urine by LC-DAD, LC-ESI-MS, and LC-ESI-MS-MS.
A case of self poisoning with metobromuron, a urea derivative used as a herbicide, is reported. Severe methemoglobinemia observed at the admission (80%) disappeared only at day 11, and hemolysis appeared at day 4 and decreased slowly to day 12. Metobromuron was analyzed by liquid chromatography with diode-array detection. Initial plasma concentration and elimination half-life were 4.9 mg/L and 5 h, respectively. Several metabolites were also detected, and four of those were identified by liquid chromatography-electrospray mass spectrometry. Normetobromuron, bromophenylurea, and bromoacetanilide were detected in plasma, but only N-methyl bromophenylurea was detected in urine. Bromoacetanilide probably results from acetylation of the intermediate bromoaniline. Methemoglobinemia could result from metabolization of metobromuron to bromoaniline and bromoacetanilide.
Turcant
A
A
Laboratoire de Pharmacologie-Toxicologie, CHU, Angers, France.
Cailleux
A
A
Le Bouil
A
A
Allain
P
P
Harry
P
P
Renault
A
A
D000083:Acetanilides / Q000032:analysis*; D000208:Acute Disease; D000328:Adult; D000814:Aniline Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006207:Half-Life; D006540:Herbicides / Q000493:pharmacokinetics / Q000506:poisoning*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008708:Methemoglobinemia / Q000097:blood / Q000139:chemically induced* / Q000652:urine; D015394:Molecular Structure; D010671:Phenylurea Compounds / Q000493:pharmacokinetics / Q000506:poisoning*; D013056:Spectrophotometry, Ultraviolet; D013406:Suicide, Attempted
D000083:Acetanilides; D000814:Aniline Compounds; D006540:Herbicides; D010671:Phenylurea Compounds; C023621:4-bromoaniline; C056005:bromoacetanilide; C008770:metobromuron