title: Two distinct binding affinities of poliovirus for its cellular receptor.
abstract: To study the kinetics and equilibrium of poliovirus binding to the poliovirus receptor, we used surface plasmon resonance to examine the interaction of a soluble form of the receptor with poliovirus. Soluble receptor purified from mammalian cells is able to bind poliovirus, neutralize viral infectivity, and induce structural changes in the virus particle. Binding studies revealed that there are two binding sites for the receptor on the poliovirus type 1 capsid, with affinity constants at 20 degrees C of K(D)(1) = 0.67 microm and K(D)(2) = 0.11 microm. The relative abundance of the two binding sites varies with temperature. At 20 degrees C, the K(D)(2) site constitutes approximately 46% of the total binding sites on the sensor chip, and its relative abundance decreased with decreasing temperature such that at 5 degrees C, the relative abundance of the K(D)(2) site is only 12% of the total binding sites. Absolute levels of the K(D)(1) site remained relatively constant at all temperatures tested. The two binding sites may correspond to docking sites for domain 1 of the receptor on the viral capsid, as predicted by a model of the poliovirus-receptor complex. Alternatively, the binding sites may be a consequence of structural breathing, or could result from receptor-induced conformational changes in the virus.
authors.affiliation: Department of Microbiology, Columbia University College of Physicians & Surgeons, New York, New York 10032, USA.
authors.forename: B M
authors.initials: BM
authors.lastname: McDermott
authors.forename: A H
authors.initials: AH
authors.lastname: Rux
authors.forename: R J
authors.initials: RJ
authors.lastname: Eisenberg
authors.forename: G H
authors.initials: GH
authors.lastname: Cohen
authors.forename: V R
authors.initials: VR
authors.lastname: Racaniello
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D006801:Humans; D008561:Membrane Fusion; D008958:Models, Molecular; D017955:Poliovirus / Q000472:pathogenicity / Q000502:physiology*; D011991:Receptors, Virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D020349:Surface Plasmon Resonance
chemical_list: D017931:DNA Primers; D011991:Receptors, Virus