Targeting of the gene product encoded by ORF UL56 of human cytomegalovirus into viral replication centers.
The highly conserved DNA-binding protein pUL56 of human cytomegalovirus (HCMV) was found to be predominantly localized throughout the nucleus as well as in viral replication centers of infected cells. The latter localization was abolished by phosphono acetic acid, an inhibitor of viral DNA replication. Immunofluorescence revealed that pUL56 co-localized in replication centers alongside pUL112-113 and pUL44 at late times of infection. By co-immunoprecipitations, a direct interaction with pUL44, a protein of the replication fork, was detected. These results showed for the first time that HCMV pUL56 is localized in viral replication centers, implicating that DNA replication is coupled with packaging.
Giesen
K
K
Institute of Virology, Robert-Koch-Str. 17, 35037, Marburg, Germany.
Radsak
K
K
Bogner
E
E
D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000821:virology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics*; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D011233:Precipitin Tests; D011485:Protein Binding; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly; D014779:Virus Replication / Q000187:drug effects
D004268:DNA-Binding Proteins; C072217:ICP36 protein, Cytomegalovirus; C090238:UL56 protein, cytomegalovirus; D014764:Viral Proteins; D015678:Viral Structural Proteins; D010746:Phosphonoacetic Acid