title: Inhibitory effects of of L-2'-deoxyguanosine 5'-triphosphate (L-dGTP) and L-2'-deoxythymidine 5'-triphosphate (L-dTTP) on human telomerase.
abstract: Telomerase, which synthesizes telomeric DNA in eukaryotic cells, is classified as a reverse transcriptase. To clarify the recognition of 2'-deoxyribonucleoside 5'-triphosphate (dNTP) chirality by telomerase, we studied the inhibitory effects of L-dGTP on HeLa cell telomerase activity using a quantitative 'stretch PCR' assay. L-dGTP had a weakly inhibitory effect (IC50 = 200 microM) in the presence of 10 microM dGTP. This effect was less obvious when the concentration of dGTP was higher. L-dTTP had a similar inhibitory effect. These findings suggest that telomerase may bind to L-dGTP and L-dTTP, and that the ability of telomerase to bind to dGTP or dTTP is changed.
authors.affiliation: Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: R
authors.initials: R
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Shudo
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: F
authors.initials: F
authors.lastname: Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Saneyoshi
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D003848:Deoxyguanine Nucleotides / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000379:methods; D013237:Stereoisomerism; D019098:Telomerase / Q000037:antagonists & inhibitors*; D013942:Thymine Nucleotides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D003848:Deoxyguanine Nucleotides; D013942:Thymine Nucleotides; C029603:deoxyguanosine triphosphate; D019098:Telomerase; C024157:thymidine 5'-triphosphate