Characterization of a novel transcription factor binding to the regulatory regions of the human pro-alpha1(I) collagen gene.
We have identified a novel transcription factor TGP (TG Binding Protein) that binds to the consensus sequence 5'-TGTGGGGTGG-3' in the promoter and intron of the human pro-alpha1(I) collagen gene. This recognition sequence, or sequences closely resembling these sequences, was also identified in the pro-alpha1(I) and pro-alpha2(I) collagen genes of other species. Competition experiments revealed that TGP is related to but distinguishable from the Ap4/5 family of transcription factors and that it can be separated from Ap4/5 according to size.
Zhao
M K
MK
School for Chemistry and Biochemistry, Potchefstroom University, Potchefstroom, 2520, South Africa.
Pretorius
P J
PJ
de Vries
W N
WN
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D016384:Consensus Sequence / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D006801:Humans; D007438:Introns / Q000235:genetics; D008970:Molecular Weight; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D011347:Procollagen / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020218:Response Elements / Q000235:genetics*; D013045:Species Specificity; D013379:Substrate Specificity; D015534:Trans-Activators / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D011347:Procollagen; D015534:Trans-Activators; D014157:Transcription Factors; C055473:enhancer-binding protein AP-4; C095901:transcription factor AP-5; D004247:DNA