Accelerated solvent extraction of vitamin K1 in medical foods in conjunction with matrix solid-phase dispersion.
An extraction technique is described for vitamin K1 in medical foods, using accelerated solvent extraction (ASE) in conjunction with matrix solid-phase dispersion (MSPD). The medical food sample is treated as it would be with MSPD extraction, followed by ASE for a hands-free automated extraction. The vitamin K1 in the ASE extract is then quantitated by reversed-phase liquid chromatography with fluorescence detection. The chromatography specifications are identical to those in previous work that used MSPD only, with a limit of detection of 6.6 pg and a limit of quantitation of 22 pg on column. Recoveries, which were determined for an analyte-fortified zero control reference material for medical foods, averaged 97.6% (n = 25) for vitamin K1. The method provides a rapid, automatic, specific, and easily controlled assay for vitamin K1 in fortified medical foods with minimal solvent usage.
Chase
G W
GW
U.S. Food and Drug Administration, Southeast Regional Laboratory, Atlanta, GA 30309, USA.
Thompson
B
B
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005526:Food, Formulated / Q000032:analysis*; D012997:Solvents; D010837:Vitamin K 1 / Q000032:analysis*
D012997:Solvents; D010837:Vitamin K 1