Development of an HIV-1-dependent expression vector with the Cre/loxP system.
Previously, we used the human methionine tRNA promoter as an expression cassette for hammerhead ribozymes. The tRNA promoter driven ribozyme was targeted against the LTR portion of the HIV-1 NL4-3 strain. We constructed VSV-G-pseudotyped MuLV-based vectors expressing the ribozyme. The ribozyme expressing retrovirus vector strongly suppressed gag p24 antigen production in freshly HIV-1 infected MT-4 cells. In this study, the potential of such a molecular genetic intervention was examined by using the Cre-loxP recombination system. Site-specific excision of HIV-1 was achieved by using this model system with an acute infection. These studies represent one step toward the development of a novel antiviral strategy for the treatment of AIDS.
Habu
Y
Y
Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan.
Takeuchi
H
H
Tamura
Y
Y
Miyano-Kurosaki
N
N
Takai
K
K
Takaku
H
H
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D005822:Genetic Vectors; D016655:HIV Core Protein p24 / Q000235:genetics; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D019426:Integrases / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012358:RNA, Transfer, Met / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014764:Viral Proteins
D016655:HIV Core Protein p24; D012358:RNA, Transfer, Met; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D008156:Luciferases; C045073:Cre recombinase; D019426:Integrases