title: Structure and chromosomal localization of the mouse SNAP-23 gene.
abstract: SNAP-23 plays an important role in the regulation of vesicle trafficking in mammalian cells. In this report, we have determined the exon/intron organization of the mouse SNAP-23 gene. The SNAP-23 gene spans 31kb of the mouse genome and consists of eight exons interrupted by seven introns. The exon organization of the mouse SNAP-23 gene is identical to that of the related SNAP-25 gene in both chicken and Drosophila, suggesting that SNAP-23 arose by duplication of the SNAP-25 gene. Primer extension analysis revealed a major transcription start site approximately 112bp upstream of the translation start site. Like many ubiquitously expressed housekeeping genes, the proximal promoter region for the mouse SNAP-23 gene lacks consensus TATA and CAAT boxes. The SNAP-23 gene was localized to mouse chromosome 2 at band 2E5 using both fluorescence in-situ hybridization and radiation hybrid panel mapping studies. The identification of the structure of the mouse SNAP-23 gene reveals that the overall exon organization of SNAP-25 family members is conserved throughout evolution.
authors.affiliation: Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: V V
authors.initials: VV
authors.lastname: Vaidyanathan
authors.forename: P A
authors.initials: PA
authors.lastname: Roche
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005091:Exons; D005796:Genes / Q000235:genetics*; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D002352:Carrier Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; C100493:SNAP23 protein, human; C107017:Snap23 protein, mouse; D004247:DNA