Expression of Fos protein in adrenal preganglionic neurons following chemical stimulation of the rostral ventrolateral medulla of the rat.
The ventrolateral medulla is known to be involved in the regulation of arterial blood pressure, especially via its connections with sympathetic preganglionic neurons (SPNs) mainly located in the intermediolateral nucleus of the spinal cord. It has been shown that stimulation of the rostral part of the ventrolateral medulla (RVLM) elicits a release of catecholamines from the adrenal medulla. The aim of the present study was to demonstrate the existence of a functional pathway between the RVLM and adrenal SPNs using the combination of a retrograde tract tracing technique (cholera toxin B subunit) with the immunohistochemical detection of Fos protein following the chemical stimulation of RVLM. The data obtained showed that: (1) chemical stimulation of the RVLM induced Fos immunoreactivity in the intermediolateral nucleus and particularly in SPNs projecting to the adrenal medulla; (2) along the thoracic segments T2-T12, 26.1% of retrogradely identified adrenal SPNs were Fos-immunoreactive with the greatest percentage (30.9%) in the T8 segment. These results favored a functional control of the RVLM on adrenal SPNs which may contribute to a substantial activation of the cardiovascular system via the release of adrenal catecholamines.
Leman
S
S
Laboratoire de Neurosciences du Comportement, Université de Lille I, Villeneuve d'Ascq, France.
Viltart
O
O
Sequeira
H
H
D000311:Adrenal Glands / Q000294:innervation*; D000818:Animals; D001339:Autonomic Fibers, Preganglionic / Q000166:cytology / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D002772:Cholera Toxin / Q000493:pharmacokinetics / Q000494:pharmacology; D006224:Cricetinae; D018698:Glutamic Acid / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D016760:Proto-Oncogene Proteins c-fos; D018698:Glutamic Acid; D002772:Cholera Toxin