title: Nonstoichiometric hydroxyapatite-anionic collagen composite as support for the double sustained release of gentamicin and norfloxacin/ciprofloxacin.
abstract: This work studied the sustained release of ciprofloxacin or norfloxacin and gentamicin from nonstoichiometric hydroxyapatite (nHA) and anionic collagen composite. Within the first 24 and 48 h, the total antibiotic supply was significantly higher than the minimal inhibitory concentration required for the majority of the gram-negative bacteria. Although gentamicin was completely released from the matrix after 48 h by a normal diffusion mechanism, ciprofloxacin or norfloxacin release was characterized by a 2-phase release mechanism due to binding to nHA by complexation with calcium ion. Under the conditions studied, most of the norfloxacin or ciprofloxacin only will be disposable due to bioresorption or dissociation of the complexes. In conclusion, due to its biocompatibility nHA-anionic collagen composite may be a convenient support for the double sustained release of the antibiotics gentamicin and ciprofloxacin/norfloxacin for the control of bone infection while promoting bone tissue growth.
authors.affiliation: Department of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos-University of São Paulo, São Carlos, SP, Brazil.
authors.forename: V C
authors.initials: VC
authors.lastname: Amaro Martins
authors.forename: G
authors.initials: G
authors.lastname: Goissis
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000737:chemistry; D001424:Bacterial Infections / Q000517:prevention & control; D001672:Biocompatible Materials / Q000737:chemistry; D001673:Biodegradation, Environmental; D001847:Bone Diseases / Q000517:prevention & control; D002118:Calcium / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000737:chemistry; D003094:Collagen / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004058:Diffusion; D004337:Drug Carriers; D016503:Drug Delivery Systems; D017886:Durapatite / Q000737:chemistry*; D005839:Gentamicins / Q000008:administration & dosage* / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D008297:Male; D009643:Norfloxacin / Q000008:administration & dosage* / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D002614:Chelating Agents; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005839:Gentamicins; D002939:Ciprofloxacin; D003094:Collagen; D017886:Durapatite; D009643:Norfloxacin; D002118:Calcium