title: Adsorption of plasma proteins on polyethylene oxide-modified lipid bilayers studied by total internal reflection fluorescence.
abstract: Distearoylphophatidylcholine (DSPC) mixed with various mole percentages of polyethylene oxide (number average molecular weight 2000)-grafted distearoylphosphatidylethanolamine (PEO2000-DSPE) were deposited on DSPE-coated quartz surfaces by the Langmuir-Blodgett deposition. Structural transitions in PEO2000 from pancake to mushroom, and from mushroom to brush conformations were revealed from film balance experiments. Adsorption kinetics of proteins from 1% platelet-poor plasma (PPP) on the supported lipid bilayers were studied using intrinsic total internal reflection fluorescence. All the supported lipid bilayers exhibited over a magnitude reduction in adsorbed plasma proteins, compared with the quartz substrate. The increase of PEO2000-DSPE density in the mixed bilayers slightly increases the amount of adsorbed proteins on the bilayers.
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: R E
authors.initials: RE
authors.lastname: Marchant
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
chemical_list: D001798:Blood Proteins; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C411182:polyethylene oxide-distearoylphosphatidylethanolamine; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; C010942:1,2-distearoyllecithin