Biochemical and genetic evidence for phospholipase C activity in Mycobacterium ulcerans.
This study reports the existence of phospholipase C and D enzymatic activities in Mycobacterium ulcerans cultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine. M. ulcerans DNA sequences homologous to the genes encoding phospholipase C in Mycobacterium tuberculosis and Pseudomonas aeruginosa were identified by sequence analysis and DNA-DNA hybridization. Whether or not the phospholipase C and D enzymes of M. ulcerans plays a role in the pathogenesis of the disease needs further investigation.
Gomez
A
A
Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp, Belgium. arley@itg.be
Mve-Obiang
A
A
Vray
B
B
Remacle
J
J
Chemlal
K
K
Meyers
W M
WM
Portaels
F
F
Fonteyne
P A
PA
D004269:DNA, Bacterial; D006801:Humans; D019911:Mycobacterium ulcerans / Q000201:enzymology* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010739:Phospholipase D / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*
D004269:DNA, Bacterial; D010738:Type C Phospholipases; D010739:Phospholipase D