Time-co-ordinated control of glycogen synthase, protein phosphatase 2A and protein kinase CK2 during culture growth in Yarrowia lipolytica in relation to glycogen metabolism.
In the growth course of the lipolytic yeast Yarrowia lipolytica, the activities of protein phosphatase 2A (PP2A) and glycogen synthase (GS) rise during the exponential phase and concomitantly glycogen storage occurs in the cells. There is also an increase in the independence ratio (RI) indicating a shift from an inactive phosphorylated GS form to an active dephosphorylated GS form. During the early stationary phase, an increase in protein kinase CK2 (CK2) activity, a reversion of RI variation and a glycogen content decrease are observed. GS activity proved to be a good indicator of early culture growth phase. Experiments carried out with enzymes purified from Y. lipolytica show strong RI variations upon the action of CK2 and PP2Ac, and 32P incorporation into GS protein through phosphorylation by CK2. GS activity would be controlled by the sequential action of PP2A and CK2.
Queiroz-Claret
C
C
Laboratoire de chimie biologique, Inra, Ina-PG, Centre de biotechnologie agro-industrielle, Thiverval-Grignon, France.
Jolivet
P
P
Chardot
T
T
Bergeron
E
E
Meunier
J C
JC
D047390:Casein Kinase II; D006003:Glycogen / Q000378:metabolism*; D006006:Glycogen Synthase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D010749:Phosphoprotein Phosphatases / Q000302:isolation & purification / Q000378:metabolism*; D054648:Protein Phosphatase 2; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
D006003:Glycogen; D006006:Glycogen Synthase; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2