In vitro evaluation of open heart surgery tubing coated with heparin and lipid.
The aim of this work was to evaluate in vitro the tromboresistence, hemolysis tendency, platelet adhesion, cytotoxicity, physicochemical properties, and stability of open-heart tubing coated with fractionated heparin-benzalkonium chloride and/or lipid dipalmitoyl L-alpha-phosphatidylcholine (DPPC). The tendency for clot formation and platelet adhesion was greater in noncoated and lipid-coated tubing than in heparin-coated tubing. There were no significant differences between the hemolytic potentials of coated and noncoated tubing. The coatings were stable during the time of the experiment. The coatings did not present cytotoxicity and physicochemical alterations.
Novello
W P
WP
Laboratório de Engenharia Biomecânica, Faculdade de Engenharia Química, State University of Campinas, Campinas, São Paulo, Brazil. novello@ct.unicamp.br
Santana
M H
MH
Fonseca De Arruda
A C
AC
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001548:Benzalkonium Compounds; D006348:Cardiac Surgical Procedures / Q000295:instrumentation*; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D005112:Extracorporeal Circulation / Q000295:instrumentation*; D005343:Fibrinolytic Agents / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology; D007440:Intubation / Q000295:instrumentation; D008055:Lipids / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects; D011143:Polyvinyl Chloride; D011310:Preservatives, Pharmaceutical; D011817:Rabbits; D013499:Surface Properties; D013501:Surface-Active Agents; D013927:Thrombosis / Q000517:prevention & control
D000925:Anticoagulants; D001548:Benzalkonium Compounds; D020099:Coated Materials, Biocompatible; D005343:Fibrinolytic Agents; D008055:Lipids; D011310:Preservatives, Pharmaceutical; D013501:Surface-Active Agents; D015060:1,2-Dipalmitoylphosphatidylcholine; D011143:Polyvinyl Chloride; D006493:Heparin