Evidence that a family of miniature inverted-repeat transposable elements (MITEs) from the Arabidopsis thaliana genome has arisen from a pogo-like DNA transposon.
Sequence similarities exist between terminal inverted repeats (TIRs) of some miniature inverted-repeat transposable element (MITE) families isolated from a wide range of organisms, including plants, insects, and humans, and TIRs of DNA transposons from the pogo family. We present here evidence that one of these MITE families, previously described for Arabidopsis thaliana, is derived from a larger element encoding a putative transposase. We have named this novel class II transposon Lemi1. We show that its putative product is related to transposases of the Tc1/mariner superfamily, being closer to the pogo family. A similar truncated element was found in a tomato DNA sequence, indicating an ancient origin and/or horizontal transfer for this family of elements. These results are reminiscent of those recently reported for the human genome, where other members of the pogo family, named Tiggers, are believed to be responsible for the generation of abundant MITE-like elements in an early primate ancestor. These results further suggest that some MITE families, which are highly reiterated in plant, insect, and human genomes, could have arisen from a similar mechanism, implicating pogo-like elements.
Feschotte
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Laboratoire Ecologie Moléculaire et Faculté Sciences et Techniques Côte-Basque, Université de Pau et des Pays de l'Adour, Pau, France.
Mouchès
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D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D004251:DNA Transposable Elements; D018744:DNA, Plant / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D029721:Drosophila Proteins; D019143:Evolution, Molecular; D006801:Humans; D007313:Insecta; D008969:Molecular Sequence Data; D011323:Primates; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D019895:Transposases / Q000737:chemistry / Q000235:genetics
D004251:DNA Transposable Elements; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019895:Transposases; C117693:pogo-T protein, Drosophila