Extended in vitro selection for synthesis of novel catalytic oligonucleotide derivatives.
A new catalyst, which is composed of nonnatural ribonucleotides, was synthesized by the in vitro selection method. Nonnatural RNAs that bound to N-methylmesoporphyrin were selected from a pool of random-sequence RNAs containing 2'-amino cytidine 5'-triphosphate (2'-amino CTP) instead of CTP. The selected RNAs not only bound to the ligand, N-methylmesoporphyrin (NMM), but also catalyzed metalation reaction of porphyrin.
Ito
Y
Y
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Japan.
Kawazoe
N
N
D002384:Catalysis; D003570:Cytidine Triphosphate / Q000031:analogs & derivatives; D015723:Gene Library; D008652:Mesoporphyrins / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C409813:2'-amino-cytidine 5'-triphosphate; D008652:Mesoporphyrins; D009843:Oligoribonucleotides; D009942:Organometallic Compounds; C065420:N-methylmesoporphyrin IX; C015092:cupric acetate; D012313:RNA; D003570:Cytidine Triphosphate