Molecular cloning and characterization of a nuclear gene encoding a putative subunit of tRNA splicing endonuclease from Arabidopsis thaliana.
tRNA splicing endonuclease is required to produce mature tRNAs from intron-containing tRNA precursors. To characterize the structural features of plant endonuclease, we have isolated a cDNA and a corresponding genomic DNA clone from libraries of Arabidopsis thaliana which encode a putative subunit of the endonuclease. The gene product has an apparent mass of 27 kDa and contains a homologous domain of approximately 130 amino acids at the C-terminal region commonly found in other eucaryal and archaeal counterparts. Southern hybridization analysis of Arabidopsis genomic DNA utilizing the cDNA clone as probe indicates the presence of at least two related genes.
Akama
K
K
Department of Biological Science, Shimane University, Matsue, Japan.
Junker
V
V
Beier
H
H
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics; D012326:RNA Splicing; D012358:RNA, Transfer, Met / Q000737:chemistry / Q000235:genetics; D012365:RNA, Transfer, Tyr / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping
D046911:Macromolecular Substances; D012322:RNA Precursors; D012358:RNA, Transfer, Met; D012365:RNA, Transfer, Tyr; D011994:Recombinant Proteins; D004722:Endoribonucleases; C051012:splicing endonuclease