Avian genetic background modulates the neural crest apoptosis induced by ethanol exposure.
BACKGROUND
Ethanol-induced neural crest apoptosis likely contributes to the distinctive craniofacial phenotype that results from prenatal alcohol exposure. The mechanism responsible for this apoptosis is incompletely understood. A serendipitous change in poultry production flocks led to the discovery that, in chick, the embryo's genetic background modulates its susceptibility to ethanol-induced apoptosis.


METHODS
We examined the level of ethanol-induced neural crest apoptosis in 11 chick layer strains or crosses, using acridine orange uptake.


RESULTS
Holding the ethanol dose and exposure stage constant, strains were classified into very sensitive (Babcock ISA, HyLine W98, Babcock B300/Hampshire Red cross [BxHR]), moderately sensitive (Spafas, HyLine W36, Babcock B300), and nonresponsive (DeKalb White and Black, Shaver White and 2000, DcKalb White/Hampshire Red cross). Detailed examination of two susceptible strains (W98, BxHR) and a resistant strain (DcKalb White) revealed that the DeKalb's nonresponse was not caused by a shift in timing of apoptosis, or to a lower alcohol exposure at either time of injection or time of death. Strains had identical stage distributions at the time of injection and at apoptosis; housing and diet were held constant.


CONCLUSIONS
Factors within the embryo and/or egg environment can affect the susceptibility to ethanol-induced apoptosis. These sensitive and resistant strains will be important tools to dissect the molecular mechanism of ethanol-induced apoptosis, and for understanding how these losses affect subsequent development.
Debelak
K A
KA
Department of Nutritional Sciences, University of Wisconsin-Madison, 53706, USA.
Smith
S M
SM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D000431:Ethanol / Q000494:pharmacology*; D009432:Neural Crest / Q000187:drug effects*
D002492:Central Nervous System Depressants; D000431:Ethanol