title: The involvement of singlet oxygen in copper-phenanthroline/H2O2-induced DNA base damage: a chemiluminescent study.
abstract: Copper in the presence of excess 1,10-phenanthroline, a reducing agent, and H2O2 causes DNA base damage as well as strand breakage. We have reported in previous work that a strong chemiluminescence was followed by DNA base damage in this system, which is characteristic of guanine. In the present work, the mechanism of the chemiluminescence was studied. Results show that the luminescence was inhibited by all three classes of reactive oxygen species (*OH, O2-, (1)O2) scavengers to different degrees. Singlet oxygen scavengers showed the most powerful inhibition while the other two classes of scavengers were relatively weaker. The emission intensity in D2O was 3-fold that in H2O. Comparing the effect of scavengers on the luminescence of DNA with that of dGMP, the ratio of inhibition was similar. On the other hand, DNA breakage analysis showed that inhibition by the singlet oxygen scavenger NaN3 of strand breakage was strong and comparable to that of the scavengers of the two oxygen radicals. The results suggest that singlet oxygen may be a major factor for the chemiluminescence of guanine, while DNA strand breakage may be caused by many active species.
authors.affiliation: Institute of Biophysics, Academia Sinica, Beijing, People's Republic of China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Ma
authors.forename: E H
authors.initials: EH
authors.lastname: Cao
authors.forename: J F
authors.initials: JF
authors.lastname: Qin
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology; D004249:DNA Damage; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000633:toxicity*; D017665:Hydroxyl Radical; D008163:Luminescent Measurements; D010100:Oxygen / Q000494:pharmacology*; D010618:Phenanthrolines / Q000633:toxicity*
chemical_list: D016166:Free Radical Scavengers; D010618:Phenanthrolines; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; D010100:Oxygen; C025205:1,10-phenanthroline