title: Conversion of a polysaccharide to nitric oxide-releasing form. Dual-mechanism anticoagulant activity of diazeniumdiolated heparin.
abstract: We describe heparin/diazeniumdiolate conjugates that generate nitric oxide (NO) at physiological pH. Like the heparin from which they were prepared, they inhibit thrombin-induced blood coagulation. Unlike heparin, they can also inhibit and reverse ADP-induced platelet aggregation (as expected for an NO-releasing agent), suggesting potential utility as dual-action antithrombotics.
authors.affiliation: Intramural Research Support Program, SAIC Frederick, NCI-FCRDC, MD 21702, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Saavedra
authors.forename: D L
authors.initials: DL
authors.lastname: Mooradian
authors.forename: K A
authors.initials: KA
authors.lastname: Mowery
authors.forename: M H
authors.initials: MH
authors.lastname: Schoenfisch
authors.forename: M L
authors.initials: ML
authors.lastname: Citro
authors.forename: K M
authors.initials: KM
authors.lastname: Davies
authors.forename: M E
authors.initials: ME
authors.lastname: Meyerhoff
authors.forename: L K
authors.initials: LK
authors.lastname: Keefer
mesh_terms: D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D009569:Nitric Oxide / Q000737:chemistry*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D010975:Platelet Aggregation Inhibitors; D011134:Polysaccharides; D009569:Nitric Oxide; D006493:Heparin