title: The Golgi apparatus of the scaly green flagellate Scherffelia dubia: uncoupling of glycoprotein and polysaccharide synthesis during flagellar regeneration.
abstract: The flagella of the green alga Scherffelia dubia are covered by scales which consist of acidic polysaccharides and glycoproteins. Experimental deflagellation results in the regeneration of flagella complete with scales. During flagellar regeneration, scales are newly synthesized in the Golgi apparatus, exocytosed and deposited on the growing flagella. Flagellar regeneration is dependent upon protein synthesis and N-glycosylation, as it is blocked by cycloheximide and partially inhibited by tunicamycin. Metabolic labeling with [35S]methionine/cysteine demonstrated that scale-associated proteins were not newly synthesized during flagellar regeneration, suggesting that the proteins deposited on regenerating flagella were drawn from a pool. Quantitative immunoelectron microscopy using a monospecific antibody directed against a scale-associated protein of 126 kDa (SAP126) revealed that the pool of SAP126 was primarily located at the plasma membrane, with minor labeling of the scale reticulum and trans-Golgi cisternae, both before deflagellation and during flagellar regeneration. Since SAP126 was sequestered during flagellar regeneration into secretory vesicles together with newly synthesized scales, it is concluded that the persistent presence of SAP126 in the trans-Golgi cisternae during scale biogenesis requires retrograde transport of the protein from the plasma membrane to the Golgi apparatus.
authors.affiliation: Botanisches Institut, Lehrstuhl I, Universität zu Köln, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Perasso
authors.forename: A
authors.initials: A
authors.lastname: Grunow
authors.forename: I M
authors.initials: IM
authors.lastname: Brüntrup
authors.forename: B
authors.initials: B
authors.lastname: Bölinger
authors.forename: M
authors.initials: M
authors.lastname: Melkonian
authors.forename: B
authors.initials: B
authors.lastname: Becker
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D056890:Eukaryota / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005407:Flagella / Q000378:metabolism / Q000648:ultrastructure; D006023:Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D010940:Plant Proteins; D011134:Polysaccharides