[Evaluation of the reincubation of positive blood cultures for detection in the BACT-ALERT system for the detection of polymicrobial bacteremia].
Between February and September 1997, 6588 blood cultures at the Instituto de Cardiología y Cirugía Cardiovascular and Hospital de Niños Ricardo Gutiérrez were studied by using the Bact-Alert system (Organon Teknika) 341 contaminants and 294 episodes of bacteremia (600 samples) were analyzed. From these samples, 280 (95.3%) were monomicrobial episodes and 14 (4.7%) polymicrobial episodes. Positive blood cultures detected by the Bact-Alert system were processed and then reincubated during 7 days, when they were Gram stained and subcultured in blood agar, chocolate agar (both in 5-10% CO2), laked blood agar supplemented with hemin and vitamin K in anaerobic atmosphere (only anaerobic bottles) and CLDE (aerobic conditions). Following reincubation, 3 out of 14 polymicrobial bacteremias were detected, rising the level of detection from 3.7% to 4.7%. Taking into account the total number of bacteremias, only in 3 out of 294 (1%), a second microorganism was detected. Otherwise, in blood cultures where a contaminating microorganism was initially isolated, no further isolates representing a true bacteremia were recovered. Reincubation and terminal subculture of initially positive blood cultures did not provide relevant data in order to change therapeutic measures in the studied population. Due to the increase in costs and labor we consider that this methodology is not routinely advised.
Soloaga
R
R
Dpto. de Investigación Clínica IUCB, Fundación Favaloro, Belgrano, Buenos Aires, Argentina. rsoloaga@drewbsa.com.ar
Manganello
S
S
Francabandiera
D
D
Nagel
C
C
Squassi
V
V
Fernández
A
A
Gutfraind
Z
Z
Tokumoto
M
M
Delbart
H
H
Galanternik
L
L
Procopio
A
A
D016470:Bacteremia / Q000175:diagnosis*; D001769:Blood / Q000382:microbiology*; D006801:Humans; D008828:Microbiological Techniques; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic