title: Presynaptic inhibition and the participation of GABA(B) receptors at neuromuscular junctions of the crab Eriphia spinifrons.
abstract: Presynaptic inhibition exerted by the common inhibitor on the closer and opener muscles and by the specific inhibitor on the opener muscle was investigated in the crab Eriphia spinifrons. In the closer muscle, activation of GABA(B) receptors by baclofen reduced the mean quantal content of excitatory junctional currents by about 25%. Blocking GABA(B) receptors with CGP 55845 diminished presynaptic inhibition at a similar percentage. GABA(B) receptor-mediated presynaptic inhibition is linked to G proteins. Application of pertussis toxin eliminated about 25% of the inhibition exerted by the common inhibitory neuron. GABA(B) receptors participate in presynaptic inhibition at release boutons of the slow and the fast closer excitor at a similar percentage. In the opener muscle, presynaptic inhibition of transmitter release from the same endings of the opener excitor was about 15% stronger with the specific inhibitor than with the common inhibitor. About 10% of the presynaptic inhibition produced by either one of the two inhibitors could be abolished by blocking GABA(B) receptors. The amplitudes of the excitatory junctional currents in the opener were reduced in the presence of baclofen by about 25%, suggesting that synaptic terminals of the opener excitor are endowed with a similar percentage of GABA(B) receptors as terminals of the slow and the fast closer excitors. Baclofen had no effect on postsynaptic inhibition, indicating that GABA(B) receptors are not involved in postsynaptic neuromuscular inhibition.
authors.affiliation: Department of Biology, University of Konstanz, Germany. werner.rathmayer@uni-konstanz.de
authors.forename: W
authors.initials: W
authors.lastname: Rathmayer
authors.forename: S
authors.initials: S
authors.lastname: Djokaj
mesh_terms: D000818:Animals; D001418:Baclofen / Q000008:administration & dosage / Q000494:pharmacology; D003386:Brachyura / Q000502:physiology*; D018755:GABA Agonists / Q000008:administration & dosage / Q000494:pharmacology; D009469:Neuromuscular Junction / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000502:physiology; D018080:Receptors, GABA-B / Q000502:physiology*; D017661:Receptors, Presynaptic / Q000502:physiology*
chemical_list: D018755:GABA Agonists; D018377:Neurotransmitter Agents; D018080:Receptors, GABA-B; D017661:Receptors, Presynaptic; D001418:Baclofen