Construction of nonnatural DNA library.
Nonnatural DNA polymerase substrates which contain many kinds of modified functional groups are synthesized. The C-5 position of dUTP was modified by amino acid, saccharide. These compounds were incorporated on a DNA double strand using E. Coli DNA polymerase. This nonnatural DNA library contains a larger diversity than native DNA or RNA and has a higher chemical stability than RNA. This library will be useful for in vitro selection study, combinatorial chemistry, and the preparation of supramolecular structures.
Ebara
Y
Y
Division of Natural Environment and Bioorganic Chemistry, Faculty of Human Development and Sciences, Kobe University, Japan.
Kaihatsu
K
K
Ueji
S
S
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides; D004926:Escherichia coli / Q000201:enzymology; D015723:Gene Library; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013698:Templates, Genetic; D014544:Uridine Triphosphate / Q000031:analogs & derivatives
D017931:DNA Primers; D003856:Deoxyuracil Nucleotides; C027078:deoxyuridine triphosphate; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D014544:Uridine Triphosphate