title: A probe for the versatile analysis and characterization of N-linked oligosaccharides.
abstract: A novel fluorescent probe, 3-(acetylamino)-6-aminoacridine (AA-Ac), has been synthesized and its applicability to the analysis of picomole levels of N-linked glycans investigated. AA-Ac was found to be an excellent derivatization reagent for N-linked glycans, giving at least twice the intensity of fluorescence as its predecessor 2-aminoacridone. AA-Ac-labeled glycans were analyzed by both normal and reversed-phase HPLC. They were also amenable to enzymatic sequencing and analysis by MALDI-TOF mass spectrometry, free zone capillary electrophoresis, and capillary electrophoresis/electrospray ionization mass spectrometry.
authors.affiliation: New Frontiers Science Park, SmithKline Beecham Pharmaceuticals, Harlow, Essex, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Charlwood
authors.forename: H
authors.initials: H
authors.lastname: Birrell
authors.forename: A
authors.initials: A
authors.lastname: Gribble
authors.forename: V
authors.initials: V
authors.lastname: Burdes
authors.forename: D
authors.initials: D
authors.lastname: Tolson
authors.forename: P
authors.initials: P
authors.lastname: Camilleri
mesh_terms: D002240:Carbohydrate Sequence; D015335:Molecular Probes; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015335:Molecular Probes; D009844:Oligosaccharides