[RAPD-markers linked to the locus for resistance to the race 4 pathogen for black rot, Xanthomonas campestris pv. campestris (Pamm.) Dow., in Brassica rapa L].
Association between the RAPD markers and the resistance to race 4 of the black rot causative agent was studied in Brassica rapa L. Experiments were carried out using doubled haploid lines, obtained via crosses between the race 4-susceptible fodder turnip and resistant pak-choi, and the F2 progeny of the crosses between the doubled haploid lines with contrasting resistance. The WE(22)980 RAPD marker inherited from the pak-choi and associated with the clubroot susceptibility was also linked to the locus responsible for the resistance to race 4 of Xanthomonas campestris pv. campestris. The two other RAPD markers were linked to susceptibility to black rot. Simultaneous association of the same DNA markers with the resistance/susceptibility to two different obligate pathogens favored the hypothesis on cluster organization of the resistance genes in plants. The markers described can be used in plant breeding and in further investigation of the genetic bases of resistance in plants.
Ignatov
A N
AN
Bioengineering Center, Russian Academy of Sciences, Moscow, Russia. ignatov@biengi.ac.ru
Kuginuki
Y
Y
Suprunova
T P
TP
Pozmogova
G E
GE
Seitova
A M
AM
Dorokhov
D B
DB
Hirai
M
M
D001483:Base Sequence; D001937:Brassica / Q000235:genetics* / Q000382:microbiology; D002874:Chromosome Mapping; D017931:DNA Primers; D008040:Genetic Linkage; D005819:Genetic Markers; D019105:Random Amplified Polymorphic DNA Technique; D016959:Xanthomonas campestris / Q000472:pathogenicity*
D017931:DNA Primers; D005819:Genetic Markers