Membrane proteins and proteomics: un amour impossible?
Proteome analysis implies the ability to separate proteins as a first step prior to characterization. Thus, the overall performance of the analysis strongly depends on the performance of the separation tool, usually two-dimensional electrophoresis. This review shows how two-dimensional electrophoresis performs with membrane proteins from bacteria or animal or vegetable cells and tissues, the recent progress in this field, and it examines future prospects in this area.
Santoni
V
V
INRA, Laboratoire de biochemie et physiologie moléculaire des plantes, Montpellier, France.
Molloy
M
M
Rabilloud
T
T
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005544:Forecasting; D008565:Membrane Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*
D008565:Membrane Proteins; D020543:Proteome