title: Nuclear localization of transcription factor Sp1.
abstract: Transcription factor Sp1 is importantly related to expression of many cellular genes. In order to identify the nuclear localization signal of Sp1, various truncated regions of Sp1 were fused to green fluorescent protein (GFP) and were expressed in HeLa cell. The C-terminal region are required to localize Sp1 in the nucleus of HeLa cell.
authors.affiliation: Institute for Medicinal Resources, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Kuwahara
authors.forename: M
authors.initials: M
authors.lastname: Azumano
authors.forename: T
authors.initials: T
authors.lastname: Takeda
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000032:analysis* / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D049452:Green Fluorescent Proteins