title: Freeze-drying method for stable preservation in cellular specimens for the detection of apoptotic cells.
abstract: We describe a method of cytologic specimens for the detection of apoptotic cells by immunostaining after long-term storage (>6 mo) using TdT-mediated dUTP-biotin nick end labelling (TUNEL) technique. The application of the TUNEL method on freeze-drying cellular sample will eliminate the need for fresh material and will provide several advantages.
authors.affiliation: Second Department of Pathology, Kobe University School of Medicine, Hyogo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: S
authors.initials: S
authors.lastname: Matsuo
authors.forename: D
authors.initials: D
authors.lastname: Nagamachi
authors.forename: K
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: R
authors.initials: R
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Maeda
mesh_terms: D017209:Apoptosis; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis; D005612:Freeze Drying / Q000379:methods*; D016591:Histocytological Preparation Techniques; D006801:Humans; D020287:In Situ Nick-End Labeling; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013160:Splenic Neoplasms / Q000235:genetics / Q000473:pathology*; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D004273:DNA, Neoplasm