title: Ridge augmentation following implantation of recombinant human bone morphogenetic protein-2 in the dog.
abstract: BACKGROUND
  Recombinant human bone morphogenetic protein-2 (rhBMP-2) in an absorbable collagen sponge (ACS) carrier induces bone for reconstruction of skeletal defects. The rhBMP-2/ACS implant is prepared by administering a rhBMP-2 solution to dry ACS. Once prepared, rhBMP-2/ACS forms a moldable, cohesive, and adhesive implant. However, rhBMP-2/ACS does not have sufficient structural strength to withstand soft tissue compression at specific anatomic sites. To more fully understand the mechanisms that affect bone induction by rhBMP-2/ACS in the presence of soft tissue compression, it would be useful to have a preclinical model that appropriately simulates such circumstances in patients. This pilot study evaluated one such potential model.
  
  
  METHODS
  Bilateral, Class III alveolar defects were surgically produced in 4 adult mongrel dogs following extraction of the mandibular fourth premolars and reduction of the alveolar ridge. After an 8-week healing interval, mucoperiosteal flaps were elevated and rhBMP-2/ACS or rhBMP-2/ACS combined with hydroxyapatite (HA) was implanted into contralateral defects. The animals were euthanized at 12 weeks post-augmentation and block biopsies processed for histologic evaluation.
  
  
  RESULTS
  Limited augmentation followed implantation of rhBMP-2/ACS (0.7 +/- 0.6 mm). In contrast, sites receiving rhBMP-2/ACS/HA exhibited clinically relevant ridge augmentation (5.5 +/- 1.6 mm). Defects implanted with rhBMP-2/ACS exhibited dense trabeculation within the corpus of the reduced alveolar process. The cortices appeared intact without evidence of expansion into the defect area. Three defects receiving rhBMP-2/ACS/HA exhibited sparse bone trabeculae amidst HA particles, fibrovascular tissue, and marrow. Commonly, the HA particles were encapsulated by fibrous tissue. Some particles were observed in contact with bone.
  
  
  CONCLUSIONS
  The results suggests that rhBMP-2/ACS has limited effect alone in this augmentation model of Class III alveolar ridge defects. Inclusion of HA into the rhBMP-2 construct results in clinically relevant augmentation, however, the quality of bone is compromised.
authors.affiliation: Department of Periodontology, Universidade Federal Fluminense, Rio de Janeiro, Brazil.
authors.forename: E P
authors.initials: EP
authors.lastname: Barboza
authors.forename: M E
authors.initials: ME
authors.lastname: Duarte
authors.forename: L
authors.initials: L
authors.lastname: Geolás
authors.forename: R G
authors.initials: RG
authors.lastname: Sorensen
authors.forename: G E
authors.initials: GE
authors.lastname: Riedel
authors.forename: U M
authors.initials: UM
authors.lastname: Wikesjö
mesh_terms: D020341:Absorbable Implants; D000539:Alveolar Process / Q000473:pathology; D000540:Alveolar Ridge Augmentation / Q000379:methods*; D000541:Alveolectomy; D000818:Animals; D001641:Bicuspid / Q000601:surgery; D001672:Biocompatible Materials / Q000627:therapeutic use; D001706:Biopsy; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000627:therapeutic use; D003094:Collagen; D004195:Disease Models, Animal; D004285:Dogs; D004337:Drug Carriers; D017886:Durapatite / Q000627:therapeutic use; D006801:Humans; D008334:Mandible / Q000601:surgery*; D009061:Mouth Mucosa / Q000473:pathology; D010865:Pilot Projects; D011312:Pressure; D011994:Recombinant Proteins; D013524:Surgical Flaps; D014081:Tooth Extraction; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000627:therapeutic use*; D014945:Wound Healing
chemical_list: C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D004337:Drug Carriers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D003094:Collagen; D017886:Durapatite