title: Isolation and complete nucleotide sequence of a Chinese Sindbis-like virus.
abstract: Infection with alphaviruses is common in the Chinese population. Here we report the isolation of a Sindbis-like virus from a pool of Anopheles mosquitoes collected in Xinjiang, China during an arbovirus survey. This virus, designated XJ-160, rapidly produced cytopathic effects on mosquito and hamster cells. In addition, it was lethal to neonatal mice if inoculated intracerebrally. Serologically, XJ-160 reacted with and was neutralized by an anti-Sindbis antibody. Anti-XJ-160 antibodies were found in several cohorts of Chinese subjects. The complete 11626-base nucleotide sequence of XJ-160 was determined. XJ-160 has diverged significantly from the prototype Sindbis virus, with an 18% difference in nucleotide sequence and an 8.6% difference in amino acids; there are 11 deletions and 2 insertions, involving 99 nucleotides in total. XJ-160 is most closely linked to Kyzylagach virus isolated in Azerbaijan. Both belong to the African/European genetic lineage of Sindbis virus, albeit more distantly related to other members.
authors.affiliation: State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, People's Republic of China.
authors.forename: G D
authors.initials: GD
authors.lastname: Liang
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: G L
authors.initials: GL
authors.lastname: Zhou
authors.forename: S H
authors.initials: SH
authors.lastname: Fu
authors.forename: Q P
authors.initials: QP
authors.lastname: Li
authors.forename: F S
authors.initials: FS
authors.lastname: Li
authors.forename: H H
authors.initials: HH
authors.lastname: He
authors.forename: Q
authors.initials: Q
authors.lastname: Jin
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: B Q
authors.initials: BQ
authors.lastname: Chen
authors.forename: Y D
authors.initials: YD
authors.lastname: Hou
mesh_terms: D018354:Alphavirus Infections / Q000821:virology*; D000818:Animals; D000852:Anopheles / Q000821:virology*; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002478:Cells, Cultured; D002681:China; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D018076:DNA, Complementary; D019143:Evolution, Molecular; D016679:Genome, Viral; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012845:Sindbis Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D000914:Antibodies, Viral; D018076:DNA, Complementary; D012367:RNA, Viral