A non-radioactive method for mapping restriction fragment length polymorphic genetic markers in Anopheles gambiae.
A non-radioactive method for in situ hybridisation of Restriction Fragment Length Polymorphic (RFLP) markers to the polytene chromosome of Anopheles gambiae, the important malaria vector, which yielded good readable quality of chromosomal bands is reported. The methodology adopted was a Biotin-Streptavidin-Alkaline Phosphatase system which yielded fluorescent signals when stained with dyes such as Nitro Blue Tetrazolium and Bromo Chloro Indolyl Phosphate.
Kumar
N P
NP
Vector Control Research Centre (ICMR), Pondicherry, India.
D000818:Animals; D000852:Anopheles / Q000235:genetics*; D005819:Genetic Markers; D017403:In Situ Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D005819:Genetic Markers