title: [Isolation and study of recombinant strains of Salmonella typhimurium SL 7207, producing HBcAG and HBcAG-HBs].
abstract: Recombinant strains producing hepatitis B virus (HBcAg) core protein and chimeric core protein exposing on its surface the major immunogenic epitope of HBsAg (HBcAg-HBs) were constructed on the base of attenuated S. typhimurium SL 7202 strain. The resultant Salmonella strains produced proteins which were capable of self-assembly into virus-like particles and showed antigenic properties of both core and surface hepatitis B proteins. A single rectal immunization with recombinant S. typhimurium induced humoral and cellular immune response to HBcAg and HBsAg. Specific anti-HBcAg were detected in animal sera and intestinal tissues, which indicated the formation of specific mucosal immunity.
authors.forename: L I
authors.initials: LI
authors.lastname: Karpenko
authors.forename: G M
authors.initials: GM
authors.lastname: Ignat'ev
authors.forename: E M
authors.initials: EM
authors.lastname: Kozhina
authors.forename: E A
authors.initials: EA
authors.lastname: Kashentseva
authors.forename: V A
authors.initials: VA
authors.lastname: Poryvaeva
authors.forename: N V
authors.initials: NV
authors.lastname: Melamed
authors.forename: S I
authors.initials: SI
authors.lastname: Baĭborodin
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Smirnova
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
mesh_terms: D000818:Animals; D000939:Epitopes / Q000276:immunology; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000235:genetics*; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology
chemical_list: D000939:Epitopes; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens