[Response of the granulation tissue to acetylcholine and noradrenaline during denervation].
Granulation tissue from the wounds of back of both paws was studied in rats on 7th day of healing following crushing of right sciatic nerve and after repeated applications of 0.2 g/l of acetylcholine (Ach) and noradrenaline (NA). In control group (rats with intact nerves) applications of Ach intensified inflammatory reaction and negatively affected the state of regenerating skin tissues: the effect of NA consists in promoting fibroblastic and leukocytic reactions. Simultaneous crushing of right sciatic nerve and infliction of the wound amplified the pathologic reaction in the wounds of denervated rats as compared to that in animals who did not undergo denervation, leading to rapid ulceration. NA administration caused negative consequences in wounds both in right and left sides. Crushing of the nerve was performed 7 days prior to wound infliction and on day 7 of the study of neurodystrophic processes has shown that Ach influence on denervated and non denervated wounds was positive, while NA influence was negative. After reinnervation adreno- and cholinoreactivity of granulation tissue was partially normalized both on the right and left sides.
Prosha
M V
MV
Department of Histology and Embryology, National Medical University of the Ukraine, Kiev, Ukraine.
D000109:Acetylcholine / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D003714:Denervation; D006097:Granulation Tissue / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D006614:Hindlimb / Q000294:innervation; D008297:Male; D009638:Norepinephrine / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D012584:Sciatic Nerve / Q000502:physiology; D012867:Skin / Q000187:drug effects / Q000294:innervation / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects
D000109:Acetylcholine; D009638:Norepinephrine