Layer-specific immunocytochemical localization of GABA(B)R1a and GABA(B)R1b receptors in the rat piriform cortex.
A peculiar, layer-segregated immunoreactive distribution of GABABR1a and GABABR1b receptor antibodies is present in the piriform cortex of adult rats. The GABABR1a antibody selectively marked the neuropile in layer Ia, where afferent olfactory fibres and intrinsic GABAergic (gamma-aminobutyric acid) axons terminate on the distal apical dendrites of pyramidal neurons. The GABABR1b antibody was detected in the soma and the large basal dendrites of layer II and III neurons. The pattern of distribution observed supports the hypothesis that (presynaptic) GABABR1a receptors in the superficial molecular layer modulate neurotransmitter release in a feedforward synaptic circuit, whereas GABABR1b (postsynaptic) receptors mediate feedback inhibitory potentials on principal cells.
Princivalle
A
A
Department of Pharmacology, University of Birmingham, UK.
Spreafico
R
R
Bowery
N
N
De Curtis
M
M
D000818:Animals; D000906:Antibodies; D007150:Immunohistochemistry; D008970:Molecular Weight; D019581:Neuropil / Q000737:chemistry; D009833:Olfactory Pathways / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018080:Receptors, GABA-B / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D018080:Receptors, GABA-B