Freeze-drying method for stable preservation in cellular specimens for the detection of apoptotic cells.
We describe a method of cytologic specimens for the detection of apoptotic cells by immunostaining after long-term storage (>6 mo) using TdT-mediated dUTP-biotin nick end labelling (TUNEL) technique. The application of the TUNEL method on freeze-drying cellular sample will eliminate the need for fresh material and will provide several advantages.
Okuyama
T
T
Second Department of Pathology, Kobe University School of Medicine, Hyogo, Japan.
Matsuo
S
S
Nagamachi
D
D
Yoshikawa
K
K
Kobayashi
T
T
Takahashi
R
R
Maeda
S
S
D017209:Apoptosis; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis; D005612:Freeze Drying / Q000379:methods*; D016591:Histocytological Preparation Techniques; D006801:Humans; D020287:In Situ Nick-End Labeling; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013160:Splenic Neoplasms / Q000235:genetics / Q000473:pathology*; D014021:Tissue Preservation / Q000379:methods*
D004273:DNA, Neoplasm