title: Quantitation of flavor-related alkenylbenzenes in tobacco smoke particulate by selected ion monitoring gas chromatography-mass spectrometry.
abstract: Little is known about the possible health effects associated with inhaling alkenylbenzenes through cigarette smoking, even though these flavor-related compounds have known toxic effects in animals. We developed a rapid and sensitive solid-phase extraction (SPE) method to quantify seven alkenylbenzenes and piperonal in mainstream cigarette smoke particulate. The smoke particulate fraction of a single cigarette was collected on Cambridge filter pads, solvent extracted, concentrated, purified with SPE, and analyzed by selected ion monitoring gas chromatography-mass spectrometry. We positively identified and quantified five alkenylbenzenes compounds (eugenol, isoeugenol, methyleugenol myristicin, and elemicin) and piperonal in the smoke particulate from eight U.S. brands with mean levels (measured in triplicate) ranging from 6.6 to 4210 ng per cigarette. Additionally, complete blocking of nearly invisible ventilation holes in the cigarette filter increased 2- to 7-fold the percent transfer of alkenylbenzenes from tobacco to the particulate fraction of mainstream smoke.
authors.affiliation: Department of Health and Human Services, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA 30341-3724, USA.
authors.forename: S B
authors.initials: SB
authors.lastname: Stanfill
authors.forename: D L
authors.initials: DL
authors.lastname: Ashley
mesh_terms: D000475:Alkenes / Q000032:analysis*; D000081248:Allylbenzene Derivatives; D001547:Benzaldehydes / Q000032:analysis; D001555:Benzene Derivatives / Q000032:analysis*; D052117:Benzodioxoles; D001593:Benzyl Compounds; D004148:Dioxolanes / Q000032:analysis; D005054:Eugenol / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D011748:Pyrogallol / Q000031:analogs & derivatives / Q000032:analysis; D012451:Safrole / Q000032:analysis; D012906:Smoke / Q000032:analysis*; D013649:Taste
chemical_list: D000475:Alkenes; D000081248:Allylbenzene Derivatives; D001547:Benzaldehydes; D001555:Benzene Derivatives; D052117:Benzodioxoles; D001593:Benzyl Compounds; D004148:Dioxolanes; D012906:Smoke; D011748:Pyrogallol; C005246:myristicin; D005054:Eugenol; C002135:elemicin; C005454:piperonal; D012451:Safrole