title: [The function of anabiosis autoinductors in microorganisms under blockade of metabolism].
abstract: Alkyl-substituted hydroxybenzenes (AHBs), which are auto-inducers of microbial dormancy (d1 factors), were found to stabilize the structure of protein macromolecules and modify the catalytic activity of enzymes. In vitro experiments showed that C6-AHB at concentrations from 10(-4) to 10(-2) M, at which it occurs in the medium as a true solution and a micellar colloid, respectively, nonspecifically inhibited the activity of chymotrypsin, RNase, invertase, and glucose oxidase. C6-AHB-induced conformational alterations in protein macromolecules were due to the formation of complexes, as evidenced by differences in the fluorescence spectra of individual RNase and C6-AHB and their mixtures and in the surface tension isotherms of C6-AHB and trypsin solutions. Data on the involvement of dormancy auto-inducers in the post-translational modification of enzymes and their inhibition will provide further insight into the mechanisms of development and maintenance of dormant microbial forms.
authors.affiliation: Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Bespalov
authors.forename: A I
authors.initials: AI
authors.lastname: Kolpakov
authors.forename: N G
authors.initials: NG
authors.lastname: Loĭko
authors.forename: E V
authors.initials: EV
authors.lastname: Doroshenko
authors.forename: A L
authors.initials: AL
authors.lastname: Muliukin
authors.forename: A N
authors.initials: AN
authors.lastname: Kozlova
authors.forename: E A
authors.initials: EA
authors.lastname: Varlamova
authors.forename: B I
authors.initials: BI
authors.lastname: Kurganov
authors.forename: G I
authors.initials: GI
authors.lastname: El'-Registan
mesh_terms: D000220:Adaptation, Biological; D002384:Catalysis; D010636:Phenols / Q000378:metabolism*; D013171:Spores, Bacterial / Q000502:physiology*
chemical_list: D010636:Phenols