TNF alpha, IL-1 beta and IL-6 production by peripheral blood monocytes in patients with renal cell carcinoma.
Renal cell carcinoma (RCC) has been shown to be immunologically more labile than other types of cancer. In this study, we examined tumor necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) production of peripheral blood monocytes in 38 RCC patients. Monocytes were isolated from peripheral blood mononuclear cells by adherence to a plastic dish and cultured with lipopolysaccharide for 24 hours. The culture supernatant was obtained, and the production of TNF alpha, IL-1 beta and IL-6 was measured by ELISA. As a result, TNF alpha and IL-1 beta production was significantly higher in the high stage patients compared to the control subjects and low stage patients. When the patients were divided according to serum C-reactive protein (CRP), TNF alpha, IL-1 beta and IL-6 production was significantly higher in the CRP-positive patients compared to the control subjects and the CRP-negative patients. Overexpression of these cytokines may therefore induce a hypermetabolic status that may be a cause of malnutrition and cancer cachexia.
Ikemoto
S
S
Department of Urology, Osaka City University Medical School, Japan.
Sugimura
K
K
Yoshida
N
N
Wada
S
S
Yamamoto
K
K
Kishimoto
T
T
D001772:Blood Cell Count; D002097:C-Reactive Protein / Q000032:analysis; D002100:Cachexia / Q000209:etiology; D002292:Carcinoma, Renal Cell / Q000097:blood* / Q000150:complications / Q000473:pathology; D002448:Cell Adhesion; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007680:Kidney Neoplasms / Q000097:blood* / Q000150:complications / Q000473:pathology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009367:Neoplasm Staging; D010969:Plastics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010969:Plastics; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein