Activin promotes astrocytic differentiation of a multipotent neural stem cell line and an astrocyte progenitor cell line from murine central nervous system.
The effects of activin A were investigated on the development of a multipotent neural stem cell line (MEB5) and an astrocyte progenitor cell line (AP-16) that were established from murine central nervous system (CNS). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis demonstrated that each cell line expresses both type I and type II activin receptors and signaling molecules for activin, Smad2, Smad3, and Smad4. Activin A did not affect the proliferation of MEB5 and AP-16 cells. When each cell line was treated alone with activin A, glial fibrillary acidic protein (GFAP), a marker for astrocytes, was induced in AP-16 cells, but not in MEB5 cells. However, activin A accelerated the leukemia inhibitory factor (LIF)-induced astroglial differentiation of MEB5 cells. These results suggest that activin promotes astrocyte differentiation of CNS neural progenitors, and the competence to activin is different between multipotent stem cells and unipotent astrocyte progenitor cells.
Satoh
M
M
Animal Cell Bank, Institute for Fermentation, Osaka (IFO), 2-17-85 Juso-honmachi, Yodogawa-ku, Osaka, Japan. satoh-motonobu@ifo.or.jp
Sugino
H
H
Yoshida
T
T
D030201:Activin Receptors, Type I; D030301:Activin Receptors, Type II; D028341:Activins; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002490:Central Nervous System / Q000166:cytology* / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology; D007265:Inhibins / Q000494:pharmacology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017978:Receptors, Growth Factor / Q000235:genetics; D051899:Smad2 Protein; D051900:Smad3 Protein; D051901:Smad4 Protein; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D015534:Trans-Activators / Q000235:genetics
D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D051899:Smad2 Protein; C497783:Smad2 protein, mouse; D051900:Smad3 Protein; C497791:Smad3 protein, mouse; D051901:Smad4 Protein; C490810:Smad4 protein, mouse; D015534:Trans-Activators; D028341:Activins; D007265:Inhibins; D030201:Activin Receptors, Type I; D030301:Activin Receptors, Type II