An experimental vaccine providing heterologous protection for Leishmania species in murine model.
Five groups each of 20 BALB/C mice were immunized by heat killed L. donovani promastigotes. Two groups served as control. Each group of mice was subdivided into 2 equal subgroups and challenged with either L. donovani or L. major. The results revealed a high cross reactivity paralleled by a high cross protection between L. donovani and L. major. Heat killed L. donovani promastigotes super-passed other tested SDS-PAGE fractionated and eluted L. d. fractions identified by immune BALB/c mice in providing protection against both L. donovani and L. major. The percentage of protection (reduction in parasite burden) offered by each vaccine was as follows: 80.21%, 36.11%, 9.29%, 33.44% and 17.48% respectively against challenge infection with L. donovani and 3.012 x 10(4), 1.095 x 10(4), 6.054 x 10(2), 2.409 x 10(4), and 3.34 x 10(2), respectively against challenge infection with L. major.
Bebars
M A
MA
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
el Serougi
A O
AO
Makled
K M
KM
Mikhael
E M
EM
Abou Gamra
M M
MM
el Sherbiny
M
M
Mohareb
A W
AW
Mohammed
E A
EA
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D007893:Leishmania donovani / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control*; D007898:Leishmaniasis, Visceral / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D016052:Protozoan Vaccines
D016052:Protozoan Vaccines