Signaling states of rhodopsin. Retinal provides a scaffold for activating proton transfer switches.
The G-protein-coupled receptor rhodopsin is activated by photoconversion of its covalently bound ligand 11-cis-retinal to the agonist all-trans-retinal. After light-induced isomerization and early photointermediates, the receptor reaches a G-protein-dependent equilibrium between active and inactive conformations distinguished by the protonation of key opsin residues. In this report, we study the role of the 9-methyl group of retinal, one of the crucial steric determinants of light activation. We find that when this group is removed, the protonation equilibrium is strongly shifted to the inactive conformation. The residually formed active species is very similar to the active form of normal rhodopsin, metarhodopsin II. It has a deprotonated Schiff base, binds to the retinal G-protein transducin, and is favored at acidic pH. Our data show that the normal proton transfer reactions are inhibited in 9-demethyl rhodopsin but are still mandatory for receptor activation. We propose that retinal and its 9-methyl group act as a scaffold for opsin to adjust key proton donor and acceptor side chains for the proton transfer reactions that stabilize the active conformation. The mechanism may also be applicable to related receptors and may thus explain the partial agonism of certain ligands.
Meyer
C K
CK
Institut für Medizinische Physik und Biophysik, Humboldt Universität zu Berlin, Universtitätsklinikum Charité, Schumannstrasse 20-21, 10098 Berlin, Germany.
Bohme
M
M
Ockenfels
A
A
Gartner
W
W
Hofmann
K P
KP
Ernst
O P
OP
D000818:Animals; D019556:COS Cells; D002417:Cattle; D005123:Eye / Q000737:chemistry; D019204:GTP-Binding Proteins / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008027:Light; D008954:Models, Biological; D011522:Protons; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012172:Retinaldehyde / Q000031:analogs & derivatives / Q000737:chemistry*; D012243:Rhodopsin / Q000737:chemistry* / Q000378:metabolism*; D012545:Schiff Bases / Q000737:chemistry; D015398:Signal Transduction; D013057:Spectrum Analysis; D015293:Transducin / Q000737:chemistry; D014466:Ultraviolet Rays
C110176:9-demethylretinal; D011522:Protons; D011994:Recombinant Proteins; D012545:Schiff Bases; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D012243:Rhodopsin; D019204:GTP-Binding Proteins; D015293:Transducin; D012172:Retinaldehyde