Susceptibility of protein kinase (ORF47)-deficient varicella-zoster virus strains to anti-herpesvirus nucleosides.
To clarify whether varicella-zoster virus (VZV) protein kinase (PK; ORF47) takes part in phosphorylation of anti-herpesvirus nucleosides, thymidine kinase (TK) deficient, and PK/TK double deficient recombinant VZV strains were isolated and their susceptibility, and that of wild type and PK-deficient strains to various nucleoside analogs was evaluated. The PK-deficient VZV strains showed a sensitivity equal to that of the wild type strain against all compounds tested, including ganciclovir. This indicates that PK is not involved in phosphorylation of the tested nucleosides in VZV-infected cells.
Suzutani
T
T
Department of Microbiology, Asahikawa Medical College, Japan. suzutani@asahikawa-med.ac.jp
Ogasawara
M
M
Shibaki
T
T
Azuma
M
M
D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D014645:Herpesvirus 3, Human / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D009705:Nucleosides / Q000378:metabolism / Q000494:pharmacology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D013937:Thymidine Kinase / Q000378:metabolism; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D009705:Nucleosides; D011494:Protein Kinases; C085818:Varicella-Zoster ORF47-associated protein kinase; D013937:Thymidine Kinase