title: IBDV-induced bursal T lymphocytes inhibit mitogenic response of normal splenocytes.
abstract: We examined the suppressive activity of bursal T cells induced by infectious bursal disease virus (IBDV) in inbred (15x7) and outbred commercial specific-pathogen-free (SPF) chickens. The suppressive activity was measured by the ability of bursal and splenic T cells from IBDV-infected chickens to inhibit mitogenic responses of normal splenocytes. The bursacytes but not the splenocytes of IBDV-infected chickens inhibited the mitogenic responses of normal splenocytes. The mitogenic inhibition by the bursacytes of IBDV-infected chickens was dose-dependent. The suppression was observed both in inbred and non-inbred chickens, and thus, was non MHC-restricted. Cell-sorting experiments revealed that both CD4(+) and CD8(+) cells from the bursa of IBDV-infected chickens, as well as cell-culture supernatants conditioned by these cells, mediated suppression. Suppressor T (Ts) cells may therefore be involved in the immunosuppression induced by IBDV.
authors.affiliation: Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: I J
authors.initials: IJ
authors.lastname: Kim
authors.forename: J M
authors.initials: JM
authors.lastname: Sharma
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary*; D002060:Bursa of Fabricius / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology*; D003208:Concanavalin A / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D007243:Infectious bursal disease virus / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology*; D011897:Random Allocation; D012588:Scintillation Counting / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology*
chemical_list: D003208:Concanavalin A