The effects of cyclophosphamide on neurotransmission in the urinary bladder of Suncus murinus, the house musk shrew.
This study has shown that cyclophosphamide treatment of the insectivore Suncus murinus, causes a down regulation in both muscarinic and P2X receptors, together with a reduced responsiveness to exogenous histamine (0.3 mM) in the urinary bladder. Electrical field stimulation (70 V, 0.3 ms, 0.5-16 Hz, 10 s every 5 min) of bladders from both control and cyclophosphamide-treated animals showed identical responses. Since post-junctional alterations have been revealed by the reduced responsiveness to exogenous carbachol (0.1 microM-3 mM) and beta,gamma-methylene ATP (0.3-300 microM), it would appear that in the bladders of cyclophosphamide-treated animals there is also a pre-junctional effect, increased transmitter release compensating for the down regulation of the receptors. As the pattern of neurotransmission of the bladder of suncus more closely resembles that of human detrusor than other commonly studied laboratory animals, this insectivore appears to be a useful animal model for the study of bladder neurotransmission in pathophysiological conditions.
Mok
M H
MH
Dept. of Anatomy and Developmental Biology, University College London, Gower Street, London, UK.
Knight
G E
GE
Andrews
P L
PL
Hoyle
C H
CH
Burnstock
G
G
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology; D018679:Cholinergic Agonists / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D006632:Histamine / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D012788:Shrews; D009435:Synaptic Transmission / Q000187:drug effects*; D001743:Urinary Bladder / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism
D018906:Antineoplastic Agents, Alkylating; D018679:Cholinergic Agonists; C005147:5'-adenylyl (beta,gamma-methylene)diphosphonate; D006632:Histamine; D000255:Adenosine Triphosphate; D003520:Cyclophosphamide; D002217:Carbachol; C002630:alpha,beta-methyleneadenosine 5'-triphosphate