Statistical analysis of DNA sequencing data (1): accuracy test of DNA data by partial re-sequencing.
To qualify DNA data, we have developed a statistical method of deciding whether the DNA data has an acceptable accuracy in sequencing process. The method is to test the probability of sequencing errors, based on partial re-sequencing. The method was successfully applied to a yeast mitochondrial DNA which is previously sequenced (1). The analysis indicates that the entire sequence is very accurate although we found one base change error on the ND1 gene sequence data by a partial re-sampling. This method is applicable to any DNA data.
Yoshida
K
K
Department of Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
Kobayashi
M
M
Futagami
K
K
Fujikoshi
Y
Y
D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal; D010843:Pichia / Q000235:genetics*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000379:methods*
D004271:DNA, Fungal; D004272:DNA, Mitochondrial