Suppression of cytochrome P450 gene expression in the livers of mice with concanavalin A-induced hepatitis.
Cytochrome P450 (CYP) gene expression in the livers of mice with concanavalin A-induced hepatitis was examined. Treatment of mice with concanavalin A (10 mg/kg, i.v.) elevated plasma alanine aminotransaminase activity. In normal liver, CYP1A2, 3A and 2E1 mRNAs were expressed, and concanavalin A treatment differentially suppressed the expression of these CYP genes. Gadolinium chloride (40 mg/kg, i.p.) treatment, which inhibited the concanavalin A-induced elevation of plasma alanine aminotransferase activity without affecting concanavalin A-induced cytokine expression, counteracted the concanavalin A-induced suppression of CYP gene expression in the liver. Kupffer cell function or hepatic injury might contribute to the concanavalin A-induced suppression of CYP gene expression.
Okamoto
T
T
Research Laboratories, Nippon Chemiphar Co., Ltd., 1-22 Hikokawato, Misato, Japan.
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology*; D003208:Concanavalin A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D005682:Gadolinium / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C
D003208:Concanavalin A; D003577:Cytochrome P-450 Enzyme System; D005682:Gadolinium; C038958:gadolinium chloride