title: Simultaneous determination of hydrocortisone, dexamethasone, indomethacin, phenylbutazone and oxyphenbutazone in equine serum by high-performance liquid chromatography.
abstract: Ethyl acetate extracts of equine serum, containing 0-5 microg/ml of hydrocortisone (HYD), dexamethasone (DEX), oxyphenbutazone (OPB), indomethacin (IND), phenylbutazone (PB) and probenecid as internal standard, were evaporated with nitrogen, resuspended in methanol and analyzed by HPLC, using a C-18 column equilibrated with 51:49 acetonitrile-water, 0.1% trifluoroacetic acid, at 1 ml/min. The eluate was monitored at 254 nm. The selectivity (inter-assay C.V.<4%), sensitivity (limits of quantitation of 0.25 microg/ml for HYD, DEX and IND, 0.5 microg/ml for PB and 1 microg/ml for OPB, despite the occurrence of significant degradation of OPB and PB during the analysis) and precision (intra-assay and inter-assay C.V.'s of about 3-6 and 9-15%, respectively) of the method appeared appropriate for anti-doping control of racehorses.
authors.affiliation: Department of Pharmacology of Natural Substances and General Physiology, University of Rome La Sapienza, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Grippa
authors.forename: L
authors.initials: L
authors.lastname: Santini
authors.forename: G
authors.initials: G
authors.lastname: Castellano
authors.forename: M T
authors.initials: MT
authors.lastname: Gatto
authors.forename: M G
authors.initials: MG
authors.lastname: Leone
authors.forename: L
authors.initials: L
authors.lastname: Saso
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003907:Dexamethasone / Q000097:blood*; D006736:Horses / Q000097:blood*; D006854:Hydrocortisone / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000097:blood*; D010113:Oxyphenbutazone / Q000097:blood; D010653:Phenylbutazone / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D003907:Dexamethasone; D010653:Phenylbutazone; D010113:Oxyphenbutazone; D006854:Hydrocortisone; D007213:Indomethacin