Structure of the O-specific polysaccharide of Proteus mirabilis O11, another Proteus O-antigen containing an amide of D-galacturonic acid with L-threonine.
The O-specific polysaccharide of Proteus mirabilis O11 was studied by sugar analysis, Smith degradation, 1H and 13C NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, and 1H-detected 1H, 13C HMQC experiments. The following structure of a pentasaccharide repeating unit of the polysaccharide was established: [formual: see text] where D-GalA6LThr is N-(D-galacturonoyl)-L-threonine. ELISA with anti-P. mirabilis O11 serum showed that D-GalA6LThr is of minor importance for manifesting the O11 immunospecificity.
Arbatsky
N P
NP
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation.
Shashkov
A S
AS
Literacka
E
E
Widmalm
G
G
Kaca
W
W
Knirel
Y A
YA
D000577:Amides / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D006603:Hexuronic Acids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D013912:Threonine / Q000737:chemistry*
D000577:Amides; D006603:Hexuronic Acids; D019081:O Antigens; C410150:O-specific polysaccharide, Proteus mirabilis O11; D011134:Polysaccharides; D013912:Threonine; C007819:galacturonic acid