Comparison of two PCR techniques used in amplification of microdissected plant chromosomes from rice and wheat.
Linker adaptor-mediated PCR (LAM-PCR) and degenerate oligonucleotide primed PCR (DOP-PCR) are major ways to generate chromosome-specific libraries. In this study, these two PCR techniques were used to amplify the microdissected mitotic and meiotic chromosomes from rice and wheat. The results of amplifications were compared, and the advantages and limitations between the two techniques are presented.
Zhou
Y
Y
Institute of Genetics, Chinese Academy of Sciences, Beijing, P.R. China.
Wang
H
H
Wei
J
J
Cui
L
L
Deng
X
X
Wang
X
X
Chen
Z
Z
D015139:Blotting, Southern; D002875:Chromosomes / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D005069:Evaluation Studies as Topic; D015723:Gene Library; D012275:Oryza / Q000235:genetics*; D020161:Physical Chromosome Mapping / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant