Low extracellular Mg2+ concentrations suppress phagocytosis in vitro by alveolar macrophages from rats.
The aim of this study was to assess the effects of extracellular Mg2+ concentrations on phagocytosis in vitro by alveolar macrophages from rats. Phagocytosis was suppressed in the presence of low, but not high, Mg2+ concentrations. Vanadate, a Mg(2+)-ATPase inhibitor, suppressed phagocytosis. In the presence of a low Mg2+ concentration or vanadate, the cytosolic free Ca2+ concentration ([Ca2+]i) increased, but the cytosolic free Mg2+ concentration did not. These results suggest that low extracellular Mg2+ concentrations and vanadate suppress phagocytosis by rat alveolar macrophages by increasing [Ca2+]i.
Ishiguro
S
S
Department of Veterinary Pharmacology, Faculty of Agriculture, Kagoshima University, Japan.
Miyamoto
A
A
Tokushima
T
T
Ueda
A
A
Nishio
A
A
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014638:Vanadates / Q000494:pharmacology
D008070:Lipopolysaccharides; D014638:Vanadates; D008274:Magnesium; D002118:Calcium