Investigation of capillary electrochromatography with brush-type chiral stationary phases.
Fused-silica capillaries (100 microm ID) were packed with the (3R, 4S)-Whelk-O chiral stationary phase (CSP) bonded on 3.0 microm silica particles. The enantiomers of 41 neutral analytes containing stereogenic centers, axes or planes were examined by packed capillary electrochromatography. More than 30 of these were cleanly resolved, owing to the selectivities and efficiencies afforded by this CSP. High reproducibility with no indication of diminished performance was observed using the same capillary for hundreds of runs (including intermediate change of the buffer system) over a period of several weeks. Acetate, 2-(N-morpholino)ethanesulfonic acid, or phosphate buffers, each modified with either acetonitrile or methanol, were used as mobile phases. The influence of buffer concentration, modifier amount, temperature, applied voltage, and pH on performance of the brush-type CSP was investigated.
Wolf
C
C
Smith Kline Beecham Pharmaceuticals, King of Prussia, PA, USA.
Spence
P L
PL
Pirkle
W H
WH
Cavender
D M
DM
Derrico
E M
EM
D002021:Buffers; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013696:Temperature
D002021:Buffers; D004573:Electrolytes