Detection of Mycoplasma synoviae in poultry environment samples by culture and polymerase chain reaction.
Successful detection of Mycoplasma synoviae (MS) by culture and PCR from samples collected in the environment of experimentally infected chickens and turkeys, or under field conditions, is described. Results showed that in the experimental infection, 10/96 and 46/96 samples of food, drinking water, feathers, droppings or dust were positive by culture and Mycoplasma-PCR. In field conditions, the number of positive results for environmental samples were respectively 7/28 and 17/28. These observations highlight the high disseminating capacities of this mycoplasma and show the usefulness of the PCR method for epidemiological studies.
Marois
C
C
Unité de mycoplasmologie bactériologie, AFSSA, BP53, F 22440, Ploufragan, France. c.marois@ploufragan.afssa.fr
Dufour-Gesbert
F
F
Kempf
I
I
Oufour-Gesbert
F
F
D000818:Animals; D002645:Chickens / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004391:Dust; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005241:Feathers / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D009174:Mycoplasma / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology* / Q000635:transmission; D013047:Specific Pathogen-Free Organisms; D014132:Trachea / Q000382:microbiology; D014422:Turkeys / Q000382:microbiology*; D014871:Water Microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D004391:Dust