title: CD1c-mediated T-cell recognition of isoprenoid glycolipids in Mycobacterium tuberculosis infection.
abstract: The discovery of the CD1 antigen presentation pathway has expanded the spectrum of T-cell antigens to include lipids, but the range of natural lipid antigens and functions of CD1-restricted T cells in vivo remain poorly understood. Here we show that the T-cell antigen receptor and the CD1c protein mediate recognition of an evolutionarily conserved family of isoprenoid glycolipids whose members include essential components of protein glycosylation and cell-wall synthesis pathways. A CD1c-restricted, mycobacteria-specific T-cell line recognized two previously unknown mycobacterial hexosyl-1-phosphoisoprenoids and structurally related mannosyl-beta1-phosphodolichols. Responses to mannosyl-beta1-phosphodolichols were common among CD1c-restricted T-cell lines and peripheral blood T lymphocytes of human subjects recently infected with M. tuberculosis, but were not seen in naive control subjects. These results define a new class of broadly distributed lipid antigens presented by the CD1 system during infection in vivo and suggest an immune mechanism for recognition of senescent or transformed cells that are known to have altered dolichol lipids.
authors.affiliation: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA. bmoody@rics.bwh.harvard.edu
authors.forename: D B
authors.initials: DB
authors.lastname: Moody
authors.forename: T
authors.initials: T
authors.lastname: Ulrichs
authors.forename: W
authors.initials: W
authors.lastname: Mühlecker
authors.forename: D C
authors.initials: DC
authors.lastname: Young
authors.forename: S S
authors.initials: SS
authors.lastname: Gurcha
authors.forename: E
authors.initials: E
authors.lastname: Grant
authors.forename: J P
authors.initials: JP
authors.lastname: Rosat
authors.forename: M B
authors.initials: MB
authors.lastname: Brenner
authors.forename: C E
authors.initials: CE
authors.lastname: Costello
authors.forename: G S
authors.initials: GS
authors.lastname: Besra
authors.forename: S A
authors.initials: SA
authors.lastname: Porcelli
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D018949:Antigens, CD1 / Q000276:immunology*; D002460:Cell Line; D004287:Dolichol Monophosphate Mannose / Q000276:immunology; D005260:Female; D006017:Glycolipids / Q000276:immunology*; D006031:Glycosylation; D006801:Humans; D008297:Male; D009162:Mycobacterium avium / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011106:Polyisoprenyl Phosphates / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D018949:Antigens, CD1; D006017:Glycolipids; D011106:Polyisoprenyl Phosphates; D004287:Dolichol Monophosphate Mannose