The harpin ribozyme: the roles of ions in RNA cleavage.
The cleavage reaction of the hairpin ribozyme is facilitated by divalent metal ions, such as Mg2+, or by non-metallic polycations, such as the polyamine spermine. We show substantial enhancement of cleavage with combinations of metallic and non-metallic ions. In addition we elucidate the locations of some ion binding sites by Fenton chemistry.
Earnshaw
D J
DJ
MRC Laboratory of Molecular Biology, Cambridge, UK.
Gait
M J
MJ
D001483:Base Sequence; D001665:Binding Sites; D002412:Cations; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011073:Polyamines / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D002412:Cations; D011073:Polyamines; D016337:RNA, Catalytic; D013096:Spermine; D012313:RNA; D008274:Magnesium; D013095:Spermidine