title: Immunization of cultured juvenile rockfish Sebastes schlegeli against Microcotyle sebastis (Monogenea).
abstract: To determine whether immunization with Microcotyle sebastis antigen could induce protection against the parasite's establishment, naive juvenile rockfish were immunized by injection or immersion with whole worm antigen of M. sebastis. The infestation intensities of immunized groups following a challenge (2 wk after boosting) with 5000 M. sebastis eyed-eggs were significantly lower than those of control groups, when determined 7 wk postinfection. The fish in the groups boosted with M. sebastis antigen showed stronger protection than unboosted groups. The control group injected with FCA only showed a significantly smaller number of worms than the control group, which was immersed in PBS containing seawater. The results strongly suggest that both specific and nonspecific immune factors participate in the protection of rockfish against M. sebastis establishment.
authors.affiliation: Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, Pusan, Korea. khkim@dolphin.pknu.ac.kr
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: Y J
authors.initials: YJ
authors.lastname: Hwang
authors.forename: J B
authors.initials: JB
authors.lastname: Cho
authors.forename: S I
authors.initials: SI
authors.lastname: Park
mesh_terms: D000818:Animals; D017756:Aquaculture; D005393:Fish Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D005399:Fishes / Q000469:parasitology*; D006374:Helminthiasis, Animal / Q000276:immunology / Q000517:prevention & control; D010270:Parasite Egg Count; D014200:Trematoda / Q000276:immunology*; D014201:Trematode Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*