Local versus systemic interleukin-2: tumor formation by wild-type and B7-1-positive murine melanoma cells.
Modification of murine K1735 melanoma cells to express the immune costimulator B7-1 had no effect on tumor formation in syngeneic mice. In contrast, <40% of mice inoculated with K1735 cells modified to secrete murine interleukin-2 (IL-2) formed tumors, and no tumors formed when the K1735 cells coexpressed both murine IL-2 and B7-1. However, administration of systemic recombinant human IL-2 had no detectable effect on the formation of tumors by the B7-1-expressing K1735 cells. By contrast, admixtures of IL-2-secreting and B7-1-expressing K1735 cells formed fewer tumors than either cell type alone. Murine IL-2 was effective only when secreted locally, because the IL-2-secreting cells inoculated into the right flank did not affect the growth of the B7-1-expressing cells inoculated into the opposite flank.
Barnard
A L
AL
Institute of Virology and Immunoprophylaxis, Mittelhaeusern, Switzerland.
Farzaneh
F
F
Gäken
J
J
Darling
D
D
D000818:Animals; D018122:B7-1 Antigen / Q000096:biosynthesis* / Q000378:metabolism; D002455:Cell Division; D006131:Growth Inhibitors / Q000008:administration & dosage / Q000096:biosynthesis / Q000502:physiology; D006801:Humans; D007274:Injections, Intraperitoneal; D007376:Interleukin-2 / Q000008:administration & dosage / Q000096:biosynthesis* / Q000502:physiology; D008546:Melanoma, Experimental / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins / Q000008:administration & dosage; D014407:Tumor Cells, Cultured
D018122:B7-1 Antigen; D006131:Growth Inhibitors; D007376:Interleukin-2; D011994:Recombinant Proteins