title: Reactions and platelet increments after transfusion of platelet concentrates in plasma or an additive solution: a prospective, randomized study.
abstract: BACKGROUND
  Reactions after platelet transfusions are rather common and frequently are caused by plasma constituents. In recent developments, the preparation and storage of platelet concentrates (PCs) in a platelet additive solution (PAS-2) have been shown to result in acceptable storage conditions. A major drawback of the use of these PCs is the progressive increase of P-selectin-positive platelets during storage. The clinical benefit of transfusions of PCs in PAS-2 was studied.
  
  
  METHODS
  PCs prepared from buffy coats were suspended in either plasma or PAS-2 and stored for up to 5 days. Clinical responses were evaluated in a prospective study in 21 patients treated with intensive chemotherapy for hematologic malignancies. Eligible patients were randomly assigned to receive prophylactic transfusions of PCs prepared in either plasma or PAS-2. Reactions and CCIs were recorded after each transfusion.
  
  
  RESULTS
  The incidence of reactions in 12 patients given PCs in plasma (n = 192) was 12 percent. Transfusions to 9 patients of PCs in PAS-2 (n = 132) showed a reduction in the incidence of reactions to 5.3 percent (p<0.05). The average 1-hour and 20-hour CCIs after transfusion of PCs in plasma were 20.7 +/- 8. 5 and 11.5 +/- 8.0, respectively. CCIs after transfusion of PCs in PAS-2 were significantly lower: the average 1-hour CCI was 17.1 +/- 6.6 (p<0.001) and the average 20-hour CCI was 9.5 +/- 7.0 (p<0.05). Storage conditions of PCs were optimal: in each group, average 1-hour CCIs of both fresh and stored PCs were similar. The 20-hour CCIs after the transfusion of fresh and stored PCs in PAS-2 also were similar.
  
  
  CONCLUSIONS
  Transfusion of PCs in PAS-2 significantly reduces the incidence of reactions. The 1-hour and 20-hour CCIs after transfusion of PCs in PAS-2 were significantly lower than the CCIs after transfusion of PCs in plasma. Because storage conditions of both PCs were found to be optimal, the decrease in CCIs after transfusion of PCs prepared in PAS-2 may be caused by rapid elimination of a subpopulation of P-selectin-positive platelets from the circulation.
authors.affiliation: Sanquin Bloodbank Ijssellanden, the Netherlands. jdewildt@compuserve.com
authors.forename: J
authors.initials: J
authors.lastname: de Wildt-Eggen
authors.forename: S
authors.initials: S
authors.lastname: Nauta
authors.forename: J G
authors.initials: JG
authors.lastname: Schrijver
authors.forename: M
authors.initials: M
authors.lastname: van Marwijk Kooy
authors.forename: M
authors.initials: M
authors.lastname: Bins
authors.forename: H C
authors.initials: HC
authors.lastname: van Prooijen
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000502:physiology*; D005260:Female; D006086:Graft vs Host Disease / Q000453:epidemiology / Q000209:etiology; D019337:Hematologic Neoplasms / Q000517:prevention & control; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000166:cytology*; D010976:Platelet Count; D017713:Platelet Transfusion / Q000009:adverse effects; D011446:Prospective Studies; D012996:Solutions / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: D012996:Solutions