title: Leukotriene B4 photoaffinity probes: design, synthesis and evaluation of new arylazide-1,3-disubstituted cyclohexanes.
abstract: The synthesis and the binding affinities of new leukotriene B4 receptor photoaffinity probes, where a 1,3-disubstituted cyclohexane ring replaces the conjugated delta6,7 and delta8,9 double bonds of the natural eicosanoid, are described. One enantiomeric compound, 4b alpha, is specifically cross-linked upon photolysis to the recombinant leukotriene B4 receptor from human origin (h-BLTR) solubilized in a micellar medium. This probe appears as a good candidate for identifying the ligand binding site of this receptor.
authors.affiliation: Laboratoire de Chimie Biomoléculaire et des Interactions Biologiques, UPRESA-CNRS 5074, Faculté de Pharmacie, Montpellier, France.
authors.forename: D
authors.initials: D
authors.lastname: Durand
authors.forename: P
authors.initials: P
authors.lastname: Hullot
authors.forename: J P
authors.initials: JP
authors.lastname: Vidal
authors.forename: J P
authors.initials: JP
authors.lastname: Girard
authors.forename: J L
authors.initials: JL
authors.lastname: Banères
authors.forename: J
authors.initials: J
authors.lastname: Parello
authors.forename: A
authors.initials: A
authors.lastname: Muller
authors.forename: C
authors.initials: C
authors.lastname: Bonne
authors.forename: J C
authors.initials: JC
authors.lastname: Rossi
mesh_terms: D003510:Cyclohexanes / Q000138:chemical synthesis / Q000737:chemistry*; D015195:Drug Design; D004341:Drug Evaluation; D006801:Humans; D007975:Leukotriene B4 / Q000737:chemistry*; D015335:Molecular Probes; D019920:Photoaffinity Labels; D011994:Recombinant Proteins / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D003510:Cyclohexanes; D015335:Molecular Probes; D019920:Photoaffinity Labels; D011994:Recombinant Proteins; D007975:Leukotriene B4