Isolation and measurement of carbonic anhydrase isoenzymes in erythrocytes of dogs.
OBJECTIVE
To purify canine carbonic anhydrase (CA) isoenzymes CA-I and CA-II and to determine concentrations of CA-I and CA-II in erythrocytes of Beagles and dogs native to Japan.


METHODS
Blood samples from 116 Beagles, including 24 pregnant Beagles, and blood samples from 29 dogs native to Japan.


METHODS
Canine CA-I and CA-II were purified by use of column chromatography. Concentrations of CA-I and CA-II in erythrocytes of dogs were determined, using an ELISA.


RESULTS
Mean (+/- SD) concentrations of CA-I and CA-II in erythrocytes of Beagles were 3.21+/-0.86 and 1.63+/-0.39 mg/g of Hb, respectively. Mean concentration of CA-I was greater in male Beagles than female Beagles. In contrast, mean concentration of CA-II was greater in female Beagles than male Beagles. Furthermore, concentration of CA-II was greater in pregnant female Beagles than male or nonpregnant female Beagles. Mean concentrations of CA-I and CA-II in erythrocytes of dogs native to Japan were 11.03+/-4.39 and 3.29+/-0.91 mg/g of Hb, respectively. Mean concentration of CA-I was greater in male dogs from Japan than female dogs from Japan.


CONCLUSIONS
The ELISA used in this study proved to be precise and sensitive for determining CA-I and CA-II concentrations in dogs. The ELISA may enable study of changes in isoenzymes associated with hereditary or metabolic disorders of blood or other body fluids, using only a small sample. Measurement of the concentrations of CA isoenzymes in dogs may be of diagnostic value.
Nishita
T
T
Laboratory of Veterinary Physiology 1, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Kondo
H
H
Ishida
S
S
Ochiai
H
H
Asari
M
M
D000818:Animals; D002256:Carbonic Anhydrases / Q000302:isolation & purification*; D004285:Dogs / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D007525:Isoelectric Focusing / Q000662:veterinary; D007564:Japan; D008297:Male; D011247:Pregnancy
D002256:Carbonic Anhydrases