title: Characterization of iridoids by fast atom bombardment mass spectrometry followed by collision-induced dissociation of
abstract: Fast atom bombardment mass spectra of a series of naturally occurring and synthetically modified iridoid glycosides were studied using lithium cationization and collision-induced dissociation of the resulting [M+Li]+ ions. Lithium cationization leads to the unambiguous determination of the molecular masses of these compounds. Collision-induced dissociation of the lithiated molecular ions give valuable structural information regarding the nature of the substituent on both the aglycone and the sugar moieties. The characteristic fragmentation pathways identified are (1) elimination of neutral molecules comprising the substituents on either the aglycone or sugar moieties, (2) formation of lithiated aglycone and their fragment ions, (3) formation of lithiated sugar and their fragment ions, (4) fragmentation corresponding to the cleavage of the aglycone or sugar ring and (5) fragmentation characteristic of the substituents present in either the aglycone or sugar parts of the molecule. Elimination of two acyloxy radicals from the lithiated molecular ion is a characteristic fragmentation in the case of acyloxy derivatives.
authors.affiliation: Medicinal Chemistry Division, Central Drug Research Institute, Lucknow, India. root@cscdri.ren.nic.in
authors.initials: KP
authors.lastname: Madhusudanan
authors.initials: VT
authors.lastname: Mathad
authors.initials: SK
authors.lastname: Raj
authors.initials: AP
authors.lastname: Bhaduri