title: Fluorescent probe and permeability to cells of isopoly (S-carboxymethyl-L-cysteine) derivative of nucleic acid bases.
abstract: Isopoly(S-carboxymethyl-L-cysteine) derivatives of nucleic acid bases were found to form stable complex with oligo-DNA in vitro. Fluorescent probed isopoly(S-carboxymethyl cysteine) derivatives of nucleic acid bases were prepared as antisense oligomers. The transfection of the oligomer into cells was carried out by HVJ-liposome method. Fluorescence was observed from the cells treated with HVJ-liposome including fluorescent probed oligomers.
authors.affiliation: Materials and Life Science, Graduate School of Osaka University, Suita, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kitaoka
authors.forename: N
authors.initials: N
authors.lastname: Tohnai
authors.forename: Y
authors.initials: Y
authors.lastname: Inaki
authors.forename: T
authors.initials: T
authors.lastname: Hatae
authors.forename: T
authors.initials: T
authors.lastname: Tanabe
mesh_terms: D000818:Animals; D019556:COS Cells; D002463:Cell Membrane Permeability; D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D010455:Peptides; D054735:Phosphorothioate Oligonucleotides; D018108:Respirovirus; D014162:Transfection / Q000379:methods
chemical_list: D004337:Drug Carriers; D005452:Fluoresceins; D005456:Fluorescent Dyes; C409812:KU5G3FL; D008081:Liposomes; D016376:Oligonucleotides, Antisense; D010455:Peptides; D054735:Phosphorothioate Oligonucleotides; C036002:poly(S-carboxymethylcysteine)