title: The cytochrome bc1 and cytochrome c oxidase complexes associate to form a single supracomplex in yeast mitochondria.
abstract: The mitochondrial electron transport chain complexes are large multisubunit complexes embedded in the inner membrane. We report here that in the yeast Saccharomyces cerevisiae, the cytochrome bc(1) and cytochrome c oxidase complexes co-exist as a larger complex of approximately 1000 kDa in the mitochondrial membrane. Following solubilization with a mild detergent, the cytochrome bc(1)-cytochrome c oxidase complex remains stable. It was analyzed using the techniques of gel filtration and blue native-polyacrylamide gel electrophoresis. Direct physical association of subunits of the cytochrome bc(1) complex with those of the cytochrome c oxidase complex was verified by co-immunoprecipitation analysis. Our data indicate that the cytochrome bc(1) complex is exclusively in association with the cytochrome c oxidase complex in yeast mitochondria. We term this complex the cytochrome bc(1)-cytochrome c oxidase supracomplex.
authors.affiliation: Institut für Physiologische Chemie der Universität München, Goethestrasse 33, 80336 München, Germany.
authors.forename: C M
authors.initials: CM
authors.lastname: Cruciat
authors.forename: S
authors.initials: S
authors.lastname: Brunner
authors.forename: F
authors.initials: F
authors.lastname: Baumann
authors.forename: W
authors.initials: W
authors.lastname: Neupert
authors.forename: R A
authors.initials: RA
authors.lastname: Stuart
mesh_terms: D004579:Electron Transport; D014450:Electron Transport Complex III / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000201:enzymology; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology*; D008970:Molecular Weight; D011487:Protein Conformation; D015003:Yeasts
chemical_list: D046911:Macromolecular Substances; D003576:Electron Transport Complex IV; D014450:Electron Transport Complex III