Acceleration of DNA strand exchange by polycation comb-type copolymer.
Polycation comb-type copolymers which are composed of poly(L-lysine) backbone and dextran graft chain (PLL-graft-Dex) accelerated DNA duplex and triplex formation and stabilized under physiologically relevant condition remarkably. In this study, we have examined the ability of polycation copolymer in promoting strand exchange between duplex DNA and its complementary single-stranded DNA. It was demonstrated that the strand exchange rate was considerably accelerated by the polycation comb-type copolymer.
Kim
W J
WJ
Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama, Japan.
Ishihara
T
T
Akaike
T
T
Maruyama
A
A
D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D003911:Dextrans; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives*
D004277:DNA, Single-Stranded; D003911:Dextrans; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; C113090:poly(lysine)-graft-dextran; D011107:Polylysine; D004247:DNA; D016650:Fluorescein-5-isothiocyanate