Peritoneal macrophages from 17 alpha-ethinylestradiol-treated NMRI mice secrete transformation-specific low molecular weight proteins.
Analysis of protein secretion was performed for a macrophage-like cell line, which was established from the peritoneal cells of NMRI mice treated with 17 alpha-ethinylestradiol. The protein secretion pattern was investigated by computerized analysis of high resolution two-dimensional gel electrophoresis (2-D PAGE) and compared to that of control macrophages, intraperitoneally activated by bacterial lipopolysaccharide. The transformed cells encode a number of low molecular weight proteins (10-20 kDa), which were not observed in control cells under identical experimental conditions. In conclusion the transformation of peritoneal macrophages by 17 alpha-ethinylestradiol leads to an upregulation of polypeptides, in particular of low molecular weight proteins. A high similarity between the induced low molecular weight protein secretion by macrophages of 17 alpha-ethinylestradiol-treated and that of 2,3,7,8-tetrabromodibenzo-p-dioxin-treated mice was found.
Schlatterer
K
K
Laboratorium für Molekularbiologie (ZBC), Klinikum der Johann-Wolfgang Goethe Universität, Frankfurt/Main, Germany.
Esmaeili
A
A
Chandra
A
A
Martin
B
B
Demirhan
I
I
Schlatterer
B
B
Chandra
P
P
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D004147:Dioxins / Q000633:toxicity; D015180:Electrophoresis, Gel, Two-Dimensional; D004997:Ethinyl Estradiol / Q000633:toxicity*; D007091:Image Processing, Computer-Assisted; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013047:Specific Pathogen-Free Organisms; D013382:Subtraction Technique
D004147:Dioxins; D008070:Lipopolysaccharides; D009363:Neoplasm Proteins; C066841:2,3,7,8-tetrabromodibenzo-4-dioxin; D004997:Ethinyl Estradiol