title: Simultaneous bioconversion of glucose and xylose to ethanol by Saccharomyces cerevisiae in the presence of xylose isomerase.
abstract: Simultaneous isomerisation and fermentation (SIF) of xylose and simultaneous isomerisation and cofermentation (SICF) of a glucose/xylose mixture was carried out by Saccharomyces cerevisiae in the presence of xylose isomerase. The SIF of 50 g I(-1) xylose gave an ethanol concentration and metabolic yield of 7.5 g l(-1) and 0.36 g (g xylose consumed)(-1). These parameters improved to 13.4 g l(-1) and 0.40 respectively, when borate was added to the medium. The SICF of a mixture of 50 g l(-1) glucose and 50 g l(-1) xylose gave an ethanol concentration and metabolic yield of 29.8 g l(-1) and 0.42 respectively, in the presence of borate. Temperature modulation from 30 degrees C to 35 degrees C during fermentation further enhanced the above parameters to 39 g l(-1) and 0.45 respectively. The approach was extended to the bioconversion of sugars present in a real lignocellulose hydrolysate (peanut-shell hydrolysate) to ethanol, with a fairly good yield.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, New Delhi.
authors.forename: P
authors.initials: P
authors.lastname: Chandrakant
authors.forename: V S
authors.initials: VS
authors.lastname: Bisaria
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D010367:Arachis / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D001881:Borates / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D007536:Isomerism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000254:growth & development; D014994:Xylose / Q000378:metabolism*; D014996:Xylulose / Q000378:metabolism
chemical_list: D001881:Borates; D000431:Ethanol; D014996:Xylulose; D014994:Xylose; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose