Transposon targeting determined by resolvase.
The Mu-related transposon Tn5090, also called Tn402, was observed to be highly selective for targets clustered in or close to recombination sites of serine-type recombinases in plasmids R388 and RP1. Transposition to the par area of RP1 responded strongly to a deletion in the gene of resolvase ParA. A search in sequence databanks revealed further insertions of Tn5090/Tn402 close to different genes of resolvases. These results imply that the target selection of Tn5090 depends on a property that is shared among several serine recombinases.
Kamali-Moghaddam
M
M
Department of Pharmaceutical Biosciences, Division of Microbiology, Uppsala University, Biomedicum, SE-751 23, Uppsala, Sweden.
Sundström
L
L
D001483:Base Sequence; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D018390:Gene Targeting; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D045522:Recombinases; D014170:Transformation, Genetic; D019895:Transposases / Q000235:genetics / Q000378:metabolism*; D045564:Transposon Resolvases
D004251:DNA Transposable Elements; D045522:Recombinases; D019895:Transposases; D045564:Transposon Resolvases