title: Separation and structural analysis of some saponins from Quillaja saponaria Molina.
abstract: A fraction of saponins from Quillaja saponaria Molina, QH-B, was fractionated by consecutive separations on three different reverse-phase HPLC systems. Eight compounds were isolated and the structures of these were elucidated mainly by sugar analysis and NMR spectroscopy. The structures consisted of a quillaic acid substituted with two different trisaccharides at C-3, beta-D-Galp-(1-->2)-[alpha-L-Rhap-(1-->3)]-beta-D-GlcpA and beta-D-Galp-(1-->2)-[beta-D-Xylp-(1-->3)]-beta-D-GlcpA, and a tetra- or pentasaccharide at C-28, beta-D-Xylp-(1-->4)-[beta-D-Glcp-(1-->3)]-alpha-L-Rhap-(1--> 2)-beta-D-Fucp and beta-D-Apif-(1-->3)-beta-D-Xylp-(1-->4)-[beta-D-Glcp-(1-->3) ]-alpha-L- Rhap-(1-->2)-beta-D-Fucp. These compounds were further substituted with an acyl group either at O-3 or O-4 of the fucose residue, which is the sugar linked to C-28 of the quillaic acid.
authors.affiliation: Department of Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: N T
authors.initials: NT
authors.lastname: Nyberg
authors.forename: L
authors.initials: L
authors.lastname: Kenne
authors.forename: B
authors.initials: B
authors.lastname: Rönnberg
authors.forename: B G
authors.initials: BG
authors.lastname: Sundquist
mesh_terms: D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D019685:Rosales / Q000737:chemistry*; D012502:Sapogenins / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D012502:Sapogenins; D012503:Saponins; C005564:quillaic acid; D009828:Oleanolic Acid