title: A distinct ganglioside composition of rat pancreatic islets.
abstract: Gangliosides in rat pancreatic tissue and isolated pancreatic islets were examined by methods including glycolipid-overlay techniques. The content of gangliosides in isolated pancreatic islets was approximately 6-fold higher than that in pancreatic tissue when compared on a protein basis. While N-glycolylneuraminic acid amounted to 7.2% of total lipid-bound sialic acids of pancreatic tissue, this molecular species was not detected in that of pancreatic islets. A remarkable difference in ganglioside composition was observed between pancreatic tissue and pancreatic islets. Pancreatic tissue showed a complex ganglioside pattern with GM3 as the largest ganglioside component, whereas isolated pancreatic islets had a simpler ganglioside profile without detectable amounts of GM3 and some other components. Pancreatic gangliosides were further examined by thin-layer chromatographic immunostaining with a monoclonal antibody A2B5 that reacts specifically with c-series gangliosides. Pancreatic tissue and pancreatic islets showed almost identical ganglioside patterns consisting of GT3, GT2, GQ1c, and GP1c. The concentration of c-series gangliosides in pancreatic islets was calculated to be more than 250-fold higher than that of pancreatic tissue. These results shows that pancreatic islet cells have a distinct ganglioside composition in rat pancreas.
authors.affiliation: Department of Clinical Pharmacology and Therapeutics, University of Shizuoka, Shizuoka, Japan. saitom@ys2.u-shizuoka-ken.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002855:Chromatography, Thin Layer; D005677:G(M1) Ganglioside / Q000032:analysis / Q000145:classification / Q000276:immunology / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000032:analysis / Q000145:classification / Q000378:metabolism; D005732:Gangliosides / Q000032:analysis* / Q000145:classification / Q000276:immunology / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism; D007515:Islets of Langerhans / Q000737:chemistry*; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000378:metabolism; D009438:Neuraminic Acids / Q000032:analysis / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism; D010179:Pancreas / Q000737:chemistry* / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000911:Antibodies, Monoclonal; D005679:G(M3) Ganglioside; D005732:Gangliosides; D006017:Glycolipids; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid; D005677:G(M1) Ganglioside; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid