Effects of various saccharides on cycloinulo-oligosaccharide fructanotransferase reaction: production of beta-inulotriosyl-alpha-D-mannopyranoside and 1-O-beta-inulotriosyl-alpha-L-sorbopyranose.
The effects of various saccharides on the reaction of cycloinulo-oligosaccharide fructanotransferase with cycloinu-lohexaose were examined. In addition to beta-D-fructofuranosides and methyl alpha-D-glucopyranoside, D-mannose and L-sorbose were found to be effective acceptors in the reactions, and they enhanced the hydrolytic activity as effectively as methyl alpha-D-glucopyranoside. Hetero-tetrasaccharides were isolated as the major transfer products from both reaction mixtures. The isolates were identified by NMR spectroscopy as beta-inulotriosyl-alpha-D-mannopyranoside and 1-O-beta-inulotriosyl-alpha-L-sorbopyranose. Methyl beta-D-glucopyranoside was slightly effective and methyl alpha-D-mannopyranoside was not effective at all as the acceptor, but these saccharides strongly enhanced the hydrolytic activity. D-Glucosamine inhibited the enzyme activity.
Kawamura
M
M
Department of Biology, Osaka Kyoiku University, Japan. kawamuria@cc.osaka-kyoiku.ac.jp
Nakai
H
H
Uchiyama
T
T
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004305:Dose-Response Relationship, Drug; D006602:Hexosyltransferases / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008766:Methylmannosides / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000378:metabolism*; D013997:Time Factors
C410147:1-O-inulotriosylsorbopyranose; D008766:Methylmannosides; D009844:Oligosaccharides; C410148:inulotriosylmannopyranoside; C008466:methylmannoside; D006602:Hexosyltransferases; C062589:cycloinulo-oligosaccharide fructanotransferase