DNA hybridization sensor utilizing [Os(5,6-dmphen)3]2+ by square wave voltammetry.
DNA hybridization detection utilizing [Os(5,6-dmphen)3]2+ (tris(5,6-dimethyl-phenanthroline) osmium(II/III)) as hybridization indicator was studied, because [Os(5,6-dmphen)3]2+ has the most largest association constant (K2+) and high current density in osmium methyl substituted phenanthroline complexes. As the result, target DNA could be detected selectively ranging from 6.9 x 10(-10) to 6.9 x 10(-5) g/mL using square wave voltammetry.
Maruyama
K
K
Department of Chemical Science and Technology, The University of Tokushima, Japan.
Motonaka
J
J
Mishima
Y
Y
Nakabayashi
Y
Y
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes; D009942:Organometallic Compounds
D004277:DNA, Single-Stranded; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D009942:Organometallic Compounds; D004247:DNA