title: Neural and muscular receptors for motilin in the rabbit colon.
abstract: Motilin receptors were classically recognized in the gastroduodenal area, where they help to regulate interdigestive motility. More recently, motilin receptors were identified in the colon where their biologic significance remains unclear. We aimed here to characterize the motilin receptors of the rabbit colon. Distal colon and duodenum were obtained from sacrificed rabbits. Tissues homogenized by Polytron were submitted to differential centrifugation to obtain neural synaptosomes or smooth muscle plasma membranes enriched solutions. Motilin binding to these membranes was determined by the displacement of (125)I MOT by the native peptide MOT 1-22, or by peptide analogues MOT 1-12 [CH(2)NH](10-11) or GM-109 and by erythromycin derivative GM-611. Motilin binding capacity was maximum in colon nerves (49.5 +/- 6.5 fmol/mg protein vs. 19.9 +/- 2.5 in colon muscles or 9.4 +/- 2.8 and 6.6 +/- 1.2 in duodenal muscles and antral nerves respectively); all tissues expressed similar affinity for MOT 1-22, and the motilin agonist GM-611 bound equally to neural or muscle tissues from the rabbit colon; the synthetic antagonist MOT 1-12 [CH(2)NH](10-11) showed greater affinity for colon nerves than for colon muscles (plC50: 7.23 +/- 0.07 vs. 6.75 +/- 0.03). Similar results were obtained with the peptide antagonist GM-109; receptor affinity toward MOT 1-12 [CH(2)NH(10-11)] was always five times superior in neural tissues, whether they came from the colon or the antrum, than in muscle tissues, whether they were obtained from colon or from duodenum. Motilin receptors are found in very high concentration in nerves and in muscles from rabbit colon; specific motilin receptor subtypes are identified in nerves (N) and muscles (M) of the rabbit colon; N and M receptor subtypes seem independent of the organ location.
authors.affiliation: Centre Hospitalier de l'Université de Montréal, Hôpital Saint-Luc, Centre de Recherche, 264 Blvd. Rene-Levesque Est, Montréal, Quebec, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Miller
authors.forename: L
authors.initials: L
authors.lastname: Trudel
authors.forename: S
authors.initials: S
authors.lastname: St-Pierre
authors.forename: H
authors.initials: H
authors.lastname: Takanashi
authors.forename: P
authors.initials: P
authors.lastname: Poitras
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D003106:Colon / Q000294:innervation / Q000378:metabolism*; D004386:Duodenum / Q000294:innervation / Q000378:metabolism; D004917:Erythromycin / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005769:Gastrointestinal Motility / Q000502:physiology; D006727:Hormone Antagonists / Q000494:pharmacology; D007457:Iodine Radioisotopes; D009037:Motilin / Q000031:analogs & derivatives / Q000378:metabolism; D009130:Muscle, Smooth / Q000294:innervation / Q000378:metabolism; D010456:Peptides, Cyclic / Q000494:pharmacology; D011817:Rabbits; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism*; D018013:Receptors, Neuropeptide / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
chemical_list: C093574:GM 109; D006727:Hormone Antagonists; D007457:Iodine Radioisotopes; D010456:Peptides, Cyclic; D011964:Receptors, Gastrointestinal Hormone; D018013:Receptors, Neuropeptide; C050802:motilin receptor; D009037:Motilin; D004917:Erythromycin; C103741:mitemcinal