Diagnosis of toxoplasmosis in immunosuppressed mice using the polymerase chain reaction (PCR) and the enzyme linked immuno-sorbent assay (ELISA) techniques.
In the present study, Toxoplasma infection with the virulent and avirulent strains in both immunocompetent and immunosuppressed mice was diagnosed using the MDS ELISA for detection of CA in sera and the PCR for demonstration of DNA in livers, brains and mononuclear cells of blood. Both techniques proved to be convenient, especially in immunosuppressed mice.
Zawawy
L A
LA
Department of Parasitology, Faculty of Medicine, University of Alexandria, Egypt.
el-Temsahi
M M
MM
el-Azzouni
M Z
MZ
el-Gebaly
W M
WM
Sharaky
O A
OA
Awadalla
H N
HN
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006168:Guinea Pigs; D007165:Immunosuppression Therapy; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000276:immunology