The use of flow cytometry for the detection of CMV-specific antigen (pp65) in leukocytes of kidney recipients.
Flow cytometric assay (FCA) was used to detect a cytomegalovirus (CMV) specific antigen (pp65) in CMV-infected fibroblast cells and in leukocytes of kidney recipients. FCA distinguished clearly between the infected and non-infected fibroblast cells. Regarding transplant patients, the FCA was positive when the number of antigenemia assay (AA) positive cells was five or more per 5 x 10(4). Moreover, the percentage of antigenemia-positive cells by FCA correlated well with symptomatic CMV infections.
Essa
S
S
Department of Microbiology, Faculty of Medicine, University of Kuwait. sahar@hsc.kuniv.edu.kw
Pacsa
A S
AS
Al-Attiyah
R
R
El-Shazly
A
A
Raghupathy
R
R
Said
T
T
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000032:analysis* / Q000097:blood; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis; D005260:Female; D005347:Fibroblasts / Q000821:virology; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007668:Kidney / Q000821:virology*; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000821:virology*; D010750:Phosphoproteins / Q000032:analysis* / Q000097:blood; D011237:Predictive Value of Tests; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000097:blood
D000956:Antigens, Viral; D005456:Fluorescent Dyes; D007166:Immunosuppressive Agents; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; D016650:Fluorescein-5-isothiocyanate