title: Relationship of the substance P to indicators of host response in human gingival crevicular fluid.
abstract: BACKGROUND
  The substance P (SP) level in human gingival crevicular fluid (GCF) was studied in relation to clinical periodontal variables and to various indicators of host response in the GCF.
  
  
  METHODS
  GCF was collected from periodontal sites with gingival inflammation and shallow or moderately deep pocket in 48 subjects. The total amount of SP and the substances based on host response factors in a 30-s sample were determined by ELISA and enzymatic methods.
  
  
  RESULTS
  Significant correlation was found between SP and probing depth (r= 0.637, p<0.001), while correlation was weak between SP and either gingival (r= 0.177, p=0.23) or plaque index (r=0.008, p=0.96). SP also showed significant correlation with the indicators of host response: prostaglandin E2, aspartate aminotransferase, alkaline phosphatase, myeloperoxidase, interleukin-1beta, tumor necrosis factor-alpha, interleukin-8 and monocyte chemoattractant protein-1 (r=0.434-0.867, p<0.01-0.001).
  
  
  CONCLUSIONS
  These results indicate that neuropeptide SP in GCF may have a potential as an indicator of periodontal inflammation and the host response.
authors.affiliation: Department of Preventive Dentistry, Osaka University Faculty of Dentistry, Japan. haniokat@dent.osaka-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Hanioka
authors.forename: K
authors.initials: K
authors.lastname: Takaya
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumori
authors.forename: R
authors.initials: R
authors.lastname: Matsuse
authors.forename: S
authors.initials: S
authors.lastname: Shizukuishi
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D001219:Aspartate Aminotransferases / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D018932:Chemokine CCL2 / Q000032:analysis; D003774:Dental Plaque Index; D015232:Dinoprostone / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology; D005891:Gingivitis / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D010518:Periodontitis / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D009195:Peroxidase / Q000032:analysis; D013373:Substance P / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D015415:Biomarkers; D018932:Chemokine CCL2; D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P; D009195:Peroxidase; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D015232:Dinoprostone