title: AMP deaminase inhibitors. 4. Further N3-substituted coformycin aglycon analogues: N3-alkylmalonates as ribose 5'-monophosphate mimetics.
abstract: AMP deaminase (AMPDA) inhibitors increase the levels of extracellular adenosine and preserve intracellular adenylate pools in cellular models of ATP depletion and therefore represent a potential new class of antiischemic drugs. Recently we reported that replacement of the ribose 5'-monophosphate component of the very potent transition-state analogue AMPDA inhibitor coformycin monophosphate (1) with a simple alkylcarboxy group resulted in potent, selective, and cell-penetrating AMPDA inhibitors. Here we report that replacement of this alkylcarboxy group with an alpha-substituted alkylmalonic acid resulted in enhanced inhibitor potency. The lead compound, 3-(5, 5-dicarboxy-6-(3-(trifluoromethyl)phenyl)-n-hexyl)coformycin aglycon (21), exhibited an AMPDA K(i) of 0.029 microM which is (3 x 10(5))-fold lower than the K(M) for the natural substrate AMP. A comparison of inhibitory potencies shows that the diacid analogues with alpha-benzyl substituents are 2-10-fold more inhibitory than similar monoacid-monoester, monoester-monoamide, or diester derivatives. Finally, these diacid analogues are 2-40-fold more potent inhibitors than the corresponding monocarboxylates.
authors.affiliation: Metabasis Therapeutics Inc., 9390 Towne Centre Drive, San Diego, California 92121, USA. bookser@mbasis.com
authors.forename: B C
authors.initials: BC
authors.lastname: Bookser
authors.forename: S R
authors.initials: SR
authors.lastname: Kasibhatla
authors.forename: M D
authors.initials: MD
authors.lastname: Erion
mesh_terms: D000659:AMP Deaminase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D001665:Binding Sites; D003070:Coformycin / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry; D008314:Malonates / Q000138:chemical synthesis* / Q000737:chemistry; D018716:Molecular Mimicry; D010755:Organophosphates / Q000737:chemistry; D012266:Ribose / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D008314:Malonates; D010755:Organophosphates; D003070:Coformycin; D012266:Ribose; D000659:AMP Deaminase