Routine HLA-B genotyping with PCR-sequence-specific oligonucleotides (PCR-SSO) detects eight new alleles: B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508.
This paper describes eight new alleles (B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508) that have been found by routine HLA-B genotyping with sequence-specific oligonucleotides (SSOs). All of the new alleles have variations which cause changes in residues that occur within antigen binding pockets and T-cell recognition sites of the antigen. The new polymorphisms within these new alleles may affect the nature and specificity of peptide binding and cause differential T-cell activation, which may have an affect in transplantation.
Kennedy
C T
CT
Molecular Genetics Laboratory, Tissue Typing, Australian Red Cross Blood Service, Sydney, New South Wales, Australia. ckennedy@arcbs.redcross.org.au
Dodd
R
R
Le
T
T
Wallace
R
R
Ng
G
G
Greville
W D
WD
Kennedy
A
A
Taverniti
A
A
Moses
J H
JH
Clow
N
N
Watson
N
N
Dunckley
H
H
D000483:Alleles; D001483:Base Sequence; D018076:DNA, Complementary; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D059910:HLA-B15 Antigen; D015797:HLA-B35 Antigen / Q000235:genetics; D059947:HLA-B40 Antigen; D015795:HLA-B8 Antigen / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods
D018076:DNA, Complementary; D006680:HLA Antigens; D015235:HLA-B Antigens; D059910:HLA-B15 Antigen; D015797:HLA-B35 Antigen; D059947:HLA-B40 Antigen; C078438:HLA-B53 antigen; C082944:HLA-B55 antigen; D015795:HLA-B8 Antigen; D015345:Oligonucleotide Probes