In vitro selection of deoxyribozymes with DNA capping activity.
In vitro selection was used to isolate a series of deoxyribozymes from a pool of random-sequence DNAs that catalyze an ATP-dependent self-capping reaction. Each deoxyribozyme catalyzes the transfer of the nucleoside and alpha-phosphate moieties of ATP to the phosphate group located at its 5' terminus, thereby creating a 5',5'-pyrophosphate cap. This same pyrophosphate cap structure is formed by T4 DNA ligase during the classical process of DNA ligation. These DNA capping enzymes representative of a collection of self-processing deoxyribozymes that can be used for the directed modification of DNA.
Li
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Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Liu
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Breaker
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D000255:Adenosine Triphosphate / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D002384:Catalysis; D017124:Conserved Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism
D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D000255:Adenosine Triphosphate; D004247:DNA