title: In vitro leucocyte proliferative responses and lymphocyte sub-types in guinea pigs with genetically determined high- and low-level responsiveness to Trichostrongylus colubriformis.
abstract: In vitro leucocyte proliferative responses to parasite antigens and to mitogens as well as lymphocyte sub-types were compared in guinea pigs with genetically determined differences in their ability to express protective immunity against Trichostronylus colubriformis infection. Proliferative responses to parasite antigens were greatest in high-responder (HR) animals, but cells from low-responder (LR) animals were generally more responsive to mitogens. However, HR circulating leucocytes were more responsive to the T-cell-dependent B-cell mitogen pokeweed mitogen (PWM), and the response of HR, but not LR, cells increased during primary infection with T. colubriformis. Flow cytometry revealed significantly greater numbers of circulating B-cells in HR animals and, as observed for responsiveness to PWM, the number of circulating B-cells increased in HR, but not LR, animals during primary infection with this parasite. These findings suggest a larger and more labile population of B-cells in HR guinea pigs.
authors.affiliation: Department of Veterinary Anatomy and Pathology, The University of Sydney, NSW, Australia.
authors.forename: M H
authors.initials: MH
authors.lastname: Manjili
authors.forename: N C
authors.initials: NC
authors.lastname: Sangster
authors.forename: T L
authors.initials: TL
authors.lastname: Rothwell
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D006168:Guinea Pigs / Q000276:immunology*; D016130:Immunophenotyping / Q000662:veterinary; D007411:Intestinal Diseases, Parasitic / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D012376:Rodent Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology; D014253:Trichostrongylosis / Q000276:immunology / Q000662:veterinary*; D014254:Trichostrongylus / Q000276:immunology*