Small heat shock protein 27 (HSP27) associates with tubulin/microtubules in HeLa cells.
One of the monoclonal antibodies raised against mitotic HeLa cells (termed as mH3) recognized a 27-kDa protein and stained microtubules in the mitotic spindles of HeLa cells. Immunoscreening of a HeLa cDNA library revealed that mH3 antigen is a small heat shock protein, HSP27. Immunoprecipitation analysis using mH3 suggested that both alpha- and beta-tubulin are associated with HSP27. Further, sucrose-cushioned ultra centrifugation revealed that HSP27 is co-sedimented with taxol-stabilized microtubules. These results indicate that HSP27 associates with tubulin/microtubules in HeLa cells.
Hino
M
M
Department of Biological Science, Faculty of Science, Hiroshima University, Higashi-Hiroshima, 739-8526, Japan.
Kurogi
K
K
Okubo
M A
MA
Murata-Hori
M
M
Hosoya
H
H
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D014404:Tubulin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins; D014404:Tubulin