title: Identification of the subunit of cAMP receptor protein (CRP) that functionally interacts with CytR in CRP-CytR-mediated transcriptional repression.
abstract: At promoters of the Escherichia coli CytR regulon, the cAMP receptor protein (CRP) interacts with the repressor CytR to form transcriptionally inactive CRP-CytR-promoter or (CRP)(2)-CytR-promoter complexes. Here, using "oriented heterodimer" analysis, we show that only one subunit of the CRP dimer, the subunit proximal to CytR, functionally interacts with CytR in CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes. Our results provide information about the architecture of CRP-CytR-promoter and (CRP)(2)-CytR-promoter complexes and rule out the proposal that masking of activating region 2 of CRP is responsible for the transcriptional inactivity of the complexes.
authors.affiliation: Department of Molecular Biology, Odense University Campusvej 55, DK-5230 Odense M, Denmark. molbiol@molbiol.sdu.dk
authors.forename: K L
authors.initials: KL
authors.lastname: Meibom
authors.forename: B H
authors.initials: BH
authors.lastname: Kallipolitis
authors.forename: R H
authors.initials: RH
authors.lastname: Ebright
authors.forename: P
authors.initials: P
authors.lastname: Valentin-Hansen
mesh_terms: D001483:Base Sequence; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D012097:Repressor Proteins / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002373:Cyclic AMP Receptor Protein; C048425:CytR protein, E coli; D029968:Escherichia coli Proteins; D012097:Repressor Proteins