Evaluation of three commercially available kits for serological diagnosis of dengue haemorrhagic fever.
Seventy one acute phase serum samples collected during an epidemic of dengue hemorrhagic fever were tested by immunoblot, a rapid immunochromatographic assay and Dengue Duo ELISA for presence of anti dengue IgM and IgG antibodies. A concordance of 81.7% and 76.1% was seen between the three tests for the detection of anti-dengue IgM antibodies and IgG antibodies respectively. The rapid test takes only five minutes, can be easily carried out in most laboratories and compares well with the ELISA and the immunoblot.
Chakravarti
A
A
Department of Microbiology, Maulana Azad Medical College, New Delhi, India. docberry@hotmail.com
Gur
R
R
Berry
N
N
Mathur
M D
MD
D000328:Adult; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D011933:Reagent Kits, Diagnostic; D012698:Serologic Tests / Q000379:methods; D019595:Severe Dengue / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic