p53 gene deletions in paraffin-preserved lymphoid tumors from irradiated mice.
Experiments were performed to measure deletions in the p53 gene in paraffin-embedded tissues (tumors and control) derived from mice exposed to gamma-rays or neutrons up to 28 years ago. Deletions in exons 1, 3, 4, 5, 6, 7 and 9 were monitored by PCR and Southern blotting techniques. The results of these experiments demonstrated p53 deletions in only 1/6 spontaneous tumors but in 5/6 gamma-ray-induced and 5/6 neutron-induced tumors. Exons deleted in tumors from gamma-ray exposed mice were similar to those deleted in tumors from neutron-exposed mice. They document differences in spectra of p53 deletions in comparing spontaneous radiation-induced tumors.
Paunesku
T
T
Biosciences Division, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439-4833, USA.
Zhang
Y
Y
Gemmell
M A
MA
Woloschak
G E
GE
D000818:Animals; D015139:Blotting, Southern; D005091:Exons; D017353:Gene Deletion; D008223:Lymphoma / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010232:Paraffin; D016133:Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53; D010232:Paraffin