title: In vivo characterization of renal iron transport in the anaesthetized rat.
abstract: 1. In vivo microinjections of 55FeCl3 were made to assess renal iron (Fe2+/3+) transport in the anaesthetized rat. 2. Following microinjection into proximal convoluted tubules (PCTs), 18.5 +/- 2.9 % (mean +/- s.e.m., n = 11) of the 55Fe was recovered in the urine. This recovery was not dependent on the injection site indicating that iron is not reabsorbed across the surface convolutions of the proximal tubule. 3. Following microinjection into distal convoluted tubules (DCTs) 46.1 +/- 6.1 % (n = 8) of the injected 55Fe was recovered. Taken together the recovery data from the PCT and DCT microinjection studies indicate that the transport of iron occurs in the loop of Henle (LH) and collecting duct system. 4. In vivo luminal microperfusion was used to examine iron transport by the LH in more detail. In tubules perfused with 7 micromol l-1 55FeCl3, 52.7 +/- 8. 3 % (n = 8) of the perfused 55Fe was recovered in the collected fluid, indicating significant iron reabsorption in the LH. Addition of copper (Cu2+ as 7 micromol l-1 CuSO4), manganese (Mn2+ as 7 micromol l-1 MnSO4) or zinc (Zn2+ as 7 micromol l-1 ZnSO4) to the perfusate did not affect reabsorption of water, Na+ or K+, but increased recovery of 55Fe to 83.5 +/- 6.8 % (n = 8, P < 0.04), 75.8 +/- 5.9 (n = 6, not significant, n.s.) and 67.9 +/- 3.8; (n = 9, n.s. ), respectively. 5. Thus, iron transport in the LH can be reduced by the addition of copper or manganese to the luminal perfusate suggesting that these ions may compete with iron for a common transport pathway. However, this pathway may not be shared by zinc.
authors.affiliation: School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK. mark.wareing@man.ac.uk
authors.forename: M
authors.initials: M
authors.lastname: Wareing
authors.forename: C J
authors.initials: CJ
authors.lastname: Ferguson
authors.forename: R
authors.initials: R
authors.lastname: Green
authors.forename: D
authors.initials: D
authors.lastname: Riccardi
authors.forename: C P
authors.initials: CP
authors.lastname: Smith
mesh_terms: D000758:Anesthesia; D000818:Animals; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism; D027682:Cation Transport Proteins; D003300:Copper / Q000652:urine; D007501:Iron / Q000097:blood / Q000378:metabolism* / Q000652:urine; D007504:Iron Radioisotopes; D033862:Iron-Binding Proteins; D007668:Kidney / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008138:Loop of Henle / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008845:Microinjections; D009399:Nephrons / Q000033:anatomy & histology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014462:Ultrafiltration; D000099236:Solute Carrier Family 11, Member 2
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D003300:Copper; D007501:Iron; D007504:Iron Radioisotopes; D033862:Iron-Binding Proteins; D008565:Membrane Proteins; D000099236:Solute Carrier Family 11, Member 2