[Preparation of T-lymphoblastoid cell lines from patients with T-cell leukemia and asymptomatic carriers of HTLV-I: analysis of the viral genome status].
Three HTLV-I-infected partially interleukin-2-dependent lymphoid cell lines were derived from a patient with T-cellular leukemia (ATL) from Georgia and a carrier of HTLV-I from Sakhalin. The strains cultured in the presence of 3-5% interleukin-2 were designated as NBK-1, NBK-2, and YE-1, respectively. Immunoblotting analysis showed typical HTLV-I proteins in them except NBK-2 which expressed nontypical proteins p40K and p28-29K. Unexpectedly, leukemic cell fraction ATL/NBK from a patient contained gag proteins p19 (CA), p24 (MA), Pr53, and unidentified protein p29. Southern blot analysis of primary leukemic cells NBK showed one full-length non-defective provirus with restriction sites Sacl in both LTRs. Limited restriction map of the provirus virtually did not differ from previously described HTLV-I prototypes. Although the mechanism of abnormal protein expression remains to be determined, this event can be explained by defective provirus formation in NBK-2 cell line during coculturing of leukemic cells with human umbilical cord blood lymphocytes.
Pavlish
O A
OA
Seniuta
N B
NB
Shcherbak
L N
LN
Klitsunova
N V
NV
Shtutman
M S
MS
Pomekhin
O E
OE
Grutsevich
V E
VE
D015139:Blotting, Southern; D016679:Genome, Viral; D015490:HTLV-I Infections / Q000097:blood* / Q000821:virology; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D015458:Leukemia, T-Cell / Q000097:blood* / Q000821:virology; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics
D007376:Interleukin-2; D014764:Viral Proteins