title: Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with Toxoplasma gondii.
abstract: During chronic infection of mice with Toxoplasma gondii, gene message for IL-12p40, CD86, and the potassium channel Kv1.3 was detected in brain mononuclear cells, suggesting the presence of dendritic cells (DC) in the CNS. Consistently, cells bearing the DC markers CD11c and 33D1 were localized at inflammatory sites in the infected brain. The number of isolated CD11c+ brain cells increased until peak inflammation. The cells exhibited the surface phenotype of myeloid DC by coexpressing 33D1 and F4/80, little DEC-205, and no CD8alpha. These brain DC were mature, as indicated by high-level expression of MHC class II, CD40, CD54, CD80, and CD86. They triggered Ag-specific and primary allogeneic T cell responses at very low APC/T cell ratios. Among mononuclear cells from encephalitic brain, DC were the main producers of IL-12. Evidence for a parasite-dependent development of DC from CNS progenitors was obtained in vitro: after inoculation of primary brain cell culture with T. gondii, IL-12-secreting dendriform cells emerged, and DC marker genes were expressed. Different stimuli elicited the generation and maturation of brain DC: neutralization of parasite-induced GM-CSF prevented outgrowth of dendriform cells and concomitant release of IL-12. IL-12 production was up-regulated by external IFN-gamma but was stopped by inhibiting parasite replication. Consistently, DC isolated from GM-CSF-treated brain cell culture were activated to secrete IL-12 by exposure to parasite lysate. In sum, these results demonstrate T. gondii-induced expansion and functional maturation of DC in the CNS and, thus, highlight a mechanism that may contribute to the chronicity of the host response.
authors.affiliation: Institute for Medical Microbiology and Virology, Heinrich-Heine University, Duesseldorf, Germany. hans-georg.fischer@ni-duesseldorf.de
authors.forename: H G
authors.initials: HG
authors.lastname: Fischer
authors.forename: U
authors.initials: U
authors.lastname: Bonifas
authors.forename: G
authors.initials: G
authors.lastname: Reichmann
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D017951:Antigen Presentation; D000954:Antigens, Surface / Q000032:analysis; D001921:Brain / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D002452:Cell Count; D002453:Cell Cycle; D002454:Cell Differentiation / Q000276:immunology; D002908:Chronic Disease; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D004660:Encephalitis / Q000276:immunology / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000494:pharmacology; D018664:Interleukin-12 / Q000096:biosynthesis / Q000378:metabolism; D007519:Isoantigens / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014122:Toxoplasma / Q000254:growth & development / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology / Q000473:pathology
chemical_list: D000276:Adjuvants, Immunologic; D000954:Antigens, Surface; D007519:Isoantigens; D018664:Interleukin-12; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor