[Detection of extended spectrum beta-lactamases producing Enterobacteriaceae in feces].
A study was made of 366 feces for detection of extended spectrum beta-lactamases producing Enterobacteriaceae from feces. The selective agar was used for modified drigalski agar (Eiken Chemical Co., LTD) with 2 micrograms/ml cefotaxime (ESBL screen agar). 92 strains of Enterobacteriaceae, 41 Escherichia coli, 15 Citrobacter freundii, 13 Enterobacter cloacae, 11 Klebsiella pneumoniae, and other 12, were isolated from ESBL screen agar. And, R-plasmid that were selected by 2 micrograms/ml cefotaxime were transferred by conjugation from two of the 92 strains. These strain were E. coli TH9809927 and Proteus mirabilis TH9808262 that were amplified by "Toho-1 type" primer. The clude enzyme from two strains (donor) and transconjugants were especially hydrolysed cepharoridine and cefotaxime. Accordingly, two strains (0.5%) were detected as ESBL producers. We think that the result of our survey suggests the increase of ESBLs producing bacterial infection in Japan, and believe that there is a trend of infection of its by surveilance of the feces.
Komatsu
M
M
Department of Clinical Pathology, Tenri Hospital.
Aihara
M
M
Shimakawa
K
K
Yamanaka
T
T
Matsuo
S
S
D001431:Bacteriological Techniques; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000096:biosynthesis*
D001618:beta-Lactamases