Positive and negative elements mediate control of alternative splicing in the AMPD1 gene.
The second exon of the AMP deaminase (AMPD) 1 gene is alternatively spliced in response to stage-specific signals elaborated during myocyte differentiation. Since inheritance of the mutation in exon 2 of the AMPD1 gene has been recently shown to be associated with a better prognosis of congestive heart failure and the alternative splicing of exon 2 modulates the residual activity of AMPD1 in individuals with this mutant allele, the regulatory mechanism of alternative splicing in the AMPD1 gene is clinically intriguing. Retention or exclusion of exon 2 results from the interplay between negative and positive elements in the primary transcript. Exon 2 is intrinsically defective and difficult to recognize. Herein, we show that this property of exon 2 is the consequence of three defects; a suboptimal 3' splice acceptor site, a suboptimal 5' splice donor site and the small size of the exon. An improvement in any one of these defects relieves the masking of this exon. Further, this defective exon can only be identified in the presence of the adjacent downstream intron.
Morisaki
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Departments of Medicine and Genetics, University of Pennsylvania, Philadelphia, PA, USA.
Morisaki
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T
Kariko
K
K
Genetta
T
T
Holmes
E W
EW
D016475:3T3 Cells; D000659:AMP Deaminase / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D012313:RNA / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D011993:Recombinant Fusion Proteins; D012313:RNA; D000659:AMP Deaminase