title: Improvement of efficiency of the Ce(IV)-induced DNA scission--relationship between the kinetic parameters (k(cat) and Km) and the DNA structure.
abstract: The Michaelis constant (Km) for double-stranded DNA, single-stranded DNA, and dinucleotide hydrolysis by Ce(IV) ion are 4.4, 15, and more than 40 mM, respectively. The order of the k(cat), however, is dinucleotide >> oligonucleotides. Not only the improvement of k(cat) but also that of Km is important for the design of an efficient artificial nuclease.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Igawa
authors.forename: J
authors.initials: J
authors.lastname: Sumaoka
authors.forename: M
authors.initials: M
authors.lastname: Komiyama
mesh_terms: D001483:Base Sequence; D002563:Cerium; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D015226:Dinucleoside Phosphates / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D015226:Dinucleoside Phosphates; D009838:Oligodeoxyribonucleotides; D002563:Cerium; D004247:DNA