Bioconversion of alpha pinene to verbenone by resting cells of Aspergillus niger.
Resting cells of a locally isolated strain of Aspergillus niger caused the bioconversion of alpha pinene to verbenone. The formation of verbenone was raised from trace amounts (under screening conditions) to 3.28 mg/100 ml (equivalent to a molar yield of 16.5% conversion of the substrate) by amending the cultivation medium for the fungus. The optimal conditions were: 6 g/100 ml for the glucose concentration, a pH of 7.0, an alpha pinene concentration of 20 mg/100 ml, and a 6-h incubation period for the reaction.
Agrawal
R
R
Food Microbiology Department, Central Food Technological Research Institute, Mysore, India. micro@cscftri.ren.nic.in
Joseph
R
R
D001234:Aspergillus niger / Q000254:growth & development* / Q000378:metabolism*; D000081008:Bicyclic Monoterpenes; D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D039821:Monoterpenes; D013729:Terpenes / Q000378:metabolism*
D000081008:Bicyclic Monoterpenes; D003470:Culture Media; D039821:Monoterpenes; D013729:Terpenes; C052875:verbenone; C005451:alpha-pinene