A DNA vaccine coding for glycoprotein B of pseudorabies virus induces cell-mediated immunity in pigs and reduces virus excretion early after infection.
Glycoproteins B (gB), gC and gD of pseudorabies virus (PRV) have been implicated as important antigens in protective immunity against PRV infection. As cell-mediated immunity plays a major role in this protective immunity, we determined the significance of these glycoproteins in the actual induction of cell-mediated immunity. We vaccinated pigs with plasmid DNA constructs coding for gB, gC or gD and challenged them with the virulent NIA-3 strain of pseudorabies virus. Vaccination with plasmid DNA coding for gB induced the strongest cell-mediated immune responses including cytotoxic T cell responses, whereas plasmid DNA coding for gD induced the strongest virus neutralising antibody responses. Interestingly, vaccination with gB-DNA reduced virus excretion early after challenge infection while vaccination with gC-DNA or gD-DNA did not.This is the first study to demonstrate that DNA vaccination induces cytotoxic T cell responses in pigs and that cell-mediated immunity induced by vaccination with gB-DNA is important for the reduction of virus excretion early after challenge infection.
van Rooij
E M
EM
Departments of Mammalian Virology and Immunology, Institute for Animal Science and Health, ID-Lelystad, P.O. Box 65, 8200 AB, Lelystad, The Netherlands. e.m.a.vanrooij@id.wag-ur.nl
Haagmans
B L
BL
Glansbeek
H L
HL
de Visser
Y E
YE
de Bruin
M G
MG
Boersma
W
W
Bianchi
A T
AT
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002455:Cell Division; D003001:Cloning, Molecular; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary; D004279:DNA, Viral / Q000737:chemistry; D005434:Flow Cytometry / Q000662:veterinary; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D007111:Immunity, Cellular; D018189:Immunomagnetic Separation / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D010957:Plasmids; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D011897:Random Allocation; D012588:Scintillation Counting / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology* / Q000592:standards; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014774:Virulence
D000914:Antibodies, Viral; D004279:DNA, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C070915:glycoprotein gII, pseudorabies virus