The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation.
We have isolated the Schizosaccharomyces pombe orthologue of the Saccharomyces cerevisiae MOB1 gene in a screen designed to enrich for septation mutants. The gene is essential, and cells lacking it display a phenotype typical of septation signalling network mutants. mob1p is located on both spindle pole bodies throughout mitosis. In addition it is also co-localised with the medial ring later in mitosis, and flanks the septum as the medial ring contracts. We also demonstrate that mob1p can be precipitated from cells in a complex with the septation regulating kinase sid2p.
Salimova
E
E
Cell Cycle Control Laboratory, ISREC, Chemin des Boveresses, 1066 Epalinges, Switzerland.
Sohrmann
M
M
Fournier
N
N
Simanis
V
V
D000483:Alleles; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000032:analysis / Q000235:genetics*; D002462:Cell Membrane / Q000502:physiology*; D003080:Cold Temperature; D004268:DNA-Binding Proteins; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D008938:Mitosis / Q000502:physiology*; D009154:Mutation; D010750:Phosphoproteins / Q000032:analysis / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D012440:Saccharomyces / Q000166:cytology* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D015398:Signal Transduction / Q000502:physiology*; D008941:Spindle Apparatus / Q000502:physiology*
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C110689:MOB1 protein, S cerevisiae; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C499592:TAH11 protein, S cerevisiae; C498281:cdc16 protein, S pombe; C406818:cdt1 protein, S pombe; D011494:Protein Kinases; C113816:Sid2 protein, S pombe