Aphidicolin inhibits DNA replication in the micronucleus and blocks cytokinesis in Paramecium caudatum.
Aphidicolin, a selective inhibitor of DNA polymerase, totally blocks DNA replication in the micronucleus but not in the macronucleus of Paramecium caudatum. The ciliates no longer divide and after 4 days the DNA content of the macronucleus has increased by 64%. Concomitantly the cell volume has increased by 53%.
Sabaneyeva
E
E
Faculty of Biology, Department of Cytology and Histology, St. Petersburg State University, Universitetskaya nab., 7/9, St. Petersburg, 199034, Russia.
Tao
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W
Verbelen
J P
JP
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects* / Q000235:genetics; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004261:DNA Replication / Q000187:drug effects*; D016054:DNA, Protozoan / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry; D018613:Microscopy, Confocal; D010247:Paramecium / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D016054:DNA, Protozoan; D004791:Enzyme Inhibitors; D016590:Aphidicolin; D004257:DNA Polymerase II