Expression of UGA-containing Mycoplasma genes in Bacillus subtilis.
We used Bacillus subtilis to express UGA-containing Mycoplasma genes encoding the P30 adhesin (one UGA) of Mycoplasma pneumoniae and methionine sulfoxide reductase (two UGAs) of Mycoplasma genitalium. Due to natural UGA suppression, these Mycoplasma genes were expressed as full-length protein products, but at relatively low efficiency, in recombinant wild-type Bacillus. The B. subtilis-expressed Mycoplasma proteins appeared as single bands and not as multiple bands compared to expression in recombinant Escherichia coli. Bacillus mutants carrying mutations in the structural gene (prfB) for release factor 2 markedly enhanced the level of readthrough of UGA-containing Mycoplasma genes.
Kannan
T R
TR
Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284-7758, USA.
Baseman
J B
JB
D018829:Adhesins, Bacterial / Q000235:genetics*; D001412:Bacillus subtilis; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D051150:Methionine Sulfoxide Reductases; D016296:Mutagenesis; D009174:Mycoplasma / Q000235:genetics*; D009177:Mycoplasma pneumoniae / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism
D018829:Adhesins, Bacterial; C096205:P30 adhesin, Mycoplasma; D010454:Peptide Termination Factors; C041460:peptide chain termination release factor 2; D010088:Oxidoreductases; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase