Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypotheses of structural and sequence evolution.
Ascaridoid nematodes parasitize the gastrointestinal tract of vertebrate definitive hosts and are represented by more than 50 described genera. We used 582 nucleotides (83% of the coding sequence) of the mitochondrial gene cytochrome oxidase subunit 2, in combination with published small- and large-subunit nuclear rDNA sequences (2,557 characters) and morphological data (20 characters), to produce a phylogenetic hypothesis for representatives of this superfamily. This combined evidence phylogeny strongly supported clades that, with 1 exception, were consistent with Fagerholm's 1991 classification. Parsimony mapping of character states on the combined evidence tree was used to develop hypotheses for the evolution of morphological, life history, and amino acid characters. This analysis of character evolution revealed that certain key features that have been used by previous workers for developing taxonomic and evolutionary hypotheses represent plesiomorphic states. Cytochrome oxidase subunit 2 nucleotides show a strong compositional bias to A+T and a substitution bias to thymine. These biases are most apparent at third positions of codons and 4-fold degenerate sites, which is consistent with the nonrandom substitution pattern of A+T pressure. Despite nucleotide bias, cytochrome oxidase amino acid sequences show conservation and retention of critical functional residues, as inferred from comparisons to other organisms.
Nadler
S A
SA
Department of Nematology, University of California, Davis 95616-8668, USA.
Hudspeth
D S
DS
D000595:Amino Acid Sequence; D000818:Animals; D001199:Ascaridoidea / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D010940:Plant Proteins; C111957:cox2 protein, Glycine soja; D003576:Electron Transport Complex IV