title: PCR fingerprinting analysis for differentiation of Streptococcus pneumoniae reinfection versus relapse.
abstract: Polymerase chain reaction fingerprint profiles of isolates obtained during an episode of pneumococcal pneumonia with bacteremia differed significantly from profiles of isolates obtained from the same patient during a subsequent episode of pneumococcal meningitis with bacteremia. Polymerase chain reaction fingerprinting provides a means of differentiating new infection from relapse, and may be a simple molecular tool for comparison of Streptococcus pneumoniae isolates.
authors.affiliation: R. M. Alden Research Laboratory, Santa Monica/UCLA Medical Center, Santa Monica, CA 90404, USA. shuntger@dent.ucla.edu
authors.forename: S
authors.initials: S
authors.lastname: Hunt Gerardo
authors.forename: D M
authors.initials: DM
authors.lastname: Citron
authors.forename: E J
authors.initials: EJ
authors.lastname: Goldstein
mesh_terms: D016470:Bacteremia / Q000175:diagnosis; D016667:Bacterial Capsules / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008586:Meningitis, Pneumococcal / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D008826:Microbial Sensitivity Tests; D011018:Pneumonia, Pneumococcal / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012008:Recurrence; D012703:Serotyping; D013183:Sputum / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial