title: Cell death-associated translocation of plasma membrane components induced by CTL.
abstract: In the very early stages of target cell apoptosis induced by CTL, we found that fluorescence of labeling probes of the target plasma membrane, such as N-(3-triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridin ium dibromide (FM1-43), was translocated into intracellular membrane structures including nuclear envelope and mitochondria. This translocation was associated with the execution of CTL-mediated killing, because neither the CTL-target conjugation alone nor the binding of noncytotoxic Th2 clone with target cell was sufficient to provoke the process. Although FM1-43 translocation was observed in perforin-mediated cytotoxicity, examinations with several other dyes failed to detect the evidence for membrane damages that may cause influx of the dye. Moreover, the translocation was also observed in Fas-dependent apoptosis. These data indicate that the translocation precedes the damage of plasma membrane and intracellular organella in the course of apoptotic cell death and may represent the existence of a membrane trafficking that mediates the translocation of plasma membrane components in the early onset of apoptotic cell death.
authors.affiliation: Section for Bioimages, Division of Fundamental Research, Project Research Center, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Shirota-Someya
authors.forename: Y
authors.initials: Y
authors.lastname: Ikegami
authors.forename: H
authors.initials: H
authors.lastname: Komano
authors.forename: M H
authors.initials: MH
authors.lastname: Lee
authors.forename: C J
authors.initials: CJ
authors.lastname: Froelich
authors.forename: N
authors.initials: N
authors.lastname: Shinohara
authors.forename: H
authors.initials: H
authors.lastname: Takayama
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000276:immunology*; D053148:Caspase 3; D020169:Caspases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000235:genetics / Q000276:immunology*; D003594:Cytoplasmic Granules / Q000276:immunology / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005456:Fluorescent Dyes / Q000378:metabolism; D007425:Intracellular Membranes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000276:immunology / Q000378:metabolism; D010539:Permeability; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000235:genetics / Q000502:physiology
chemical_list: C073804:FM1 43; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D019014:fas Receptor; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases