Efp as a primary estrogen-responsive gene in human breast cancer.
We have previously isolated the efp (estrogen-responsive finger protein) that is required for the normal estrogen-induced cell proliferation. Here, we show the genomic organization of the human efp gene which consists of nine exons. The efp mRNA was expressed in human breast tumors and the estrogen-induced expression of the efp was found in MCF-7 human breast cancer cells. Moreover, efp promoter activity was enhanced through the estrogen-responsive element dependent on estrogen and estrogen receptor. These results suggest that the efp can mediate estrogen actions such as cell growth in human breast cancer as a primary responsive gene.
Ikeda
K
K
Department of Biochemistry, Saitama Medical School, 38 Morohongo, Moroyama, Iruma-gun, Saitama, Japan.
Orimo
A
A
Higashi
Y
Y
Muramatsu
M
M
Inoue
S
S
D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005091:Exons; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007438:Introns; D009154:Mutation; D009376:Neoplasms, Hormone-Dependent; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D020218:Response Elements; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D000070584:Tripartite Motif Proteins; D014407:Tumor Cells, Cultured; D044767:Ubiquitin-Protein Ligases
D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D004958:Estradiol; C084523:TRIM25 protein, human; D044767:Ubiquitin-Protein Ligases