Proto-oncogene expression during fracture repair.
The possibility that proto-oncogenes play a role in fracture repair has been investigated in this study. Closed fractures were created in the tibiae of NZW rabbits and treated with a plaster cast. At intervals of 1 and 2 weeks, sections of the fracture callus were obtained and stained using an immunocytochemical technique which localised c-myc and c-jun. Osteogenic cells of the periosteum, endosteum, Haversian canal and new bone trabeculae expressed proto-oncogenes while hypertrophic chondrocytes were generally immunoreactive negative. The results suggest that proto-oncogene expression may be involved in post-fracture osteogenesis but not in the chondrogenic processes which precede endochondral bone formation. This could be used as a tool for diagnosing slow healing in the early stages of fracture repair.
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The Glenfield Hospital, Groby Road, Leicester, UK.
D000818:Animals; D002146:Bony Callus / Q000378:metabolism; D002370:Casts, Surgical; D017102:Fracture Healing / Q000502:physiology*; D015870:Gene Expression; D007124:Immunoenzyme Techniques; D008297:Male; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D011817:Rabbits; D013978:Tibial Fractures / Q000378:metabolism* / Q000473:pathology / Q000601:surgery
D016755:Proto-Oncogene Proteins c-jun; D016271:Proto-Oncogene Proteins c-myc