title: No significant difference in antigenicity or tissue transglutaminase substrate specificity of Irish and US wheat gliadins.
abstract: The prevalence of clinical celiac disease has been shown to vary both across time and between genetically similar populations. Differences in wheat antigenicity and transglutaminase substrate properties are a possible explanation for these differences. This study assessed the antigenicity and transglutaminase substrate specificities of gliadins from regions of high and low celiac disease prevalence. Gliadin was extracted from three commercial US wheat sources and two Irish sources. SDS-PAGE and western blotting revealed minor, but significant variations in the gliadin extracts. However, ELISA showed no difference in the antigenicity of these gliadins. Transglutaminase pretreatment of gliadin resulted in no significant change in gliadin antigenicity and kinetic studies showed that the Kms of the various gliadins were very similar. Purified IgA and IgG had no effect on transglutaminase activity. In summary, minor variations in wheat gliadins are unlikely to explain the observed differences in disease expression across genetically similar populations.
authors.affiliation: Section of Gastroenterology, Boston Medical Center and Boston University School of Medicine, Massachusetts, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Keaveny
authors.forename: G D
authors.initials: GD
authors.lastname: Offner
authors.forename: E
authors.initials: E
authors.lastname: Bootle
authors.forename: D P
authors.initials: DP
authors.lastname: Nunes
mesh_terms: D000941:Antigens / Q000032:analysis*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005903:Gliadin / Q000737:chemistry* / Q000276:immunology; D007494:Ireland; D012967:Sodium Dodecyl Sulfate; D013379:Substrate Specificity; D013501:Surface-Active Agents; D011503:Transglutaminases / Q000032:analysis*; D014908:Triticum; D014481:United States
chemical_list: D000941:Antigens; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D005903:Gliadin; D011503:Transglutaminases