In vivo selection of RNA-binding peptides from combinatorial libraries.
We have used a two step procedure to identify peptides that bind strongly to the Rev-response element (RRE) of HIV. In the first step, RRE-binding peptides were screened from a combinatorial peptide library generated by "randomization" using a small subset of the 20 amino acids. In the second step, one such RRE-binding peptide, RSG-1, was "evolved" into an even stronger RRE-binding peptide using a codon-based mutagenesis procedure. After 2 rounds of evolution, RSG-1.2 bound the RRE with 7-fold higher affinity than wild-type Rev peptide.
Harada
K
K
Department of Life Science, Tokyo Gakugei University, Japan.
Martin
S S
SS
Frankel
A D
AD
D000595:Amino Acid Sequence; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D015694:Gene Products, rev / Q000737:chemistry / Q000378:metabolism; D006678:HIV; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D009687:Nuclear Proteins; D019151:Peptide Library; D010455:Peptides; C069802:nuclear factor, RRE binding; D054321:rev Gene Products, Human Immunodeficiency Virus