title: Induction of nitric oxide in bovine peripheral blood mononuclear cells by bovine herpesvirus 1.
abstract: Bovine peripheral blood mononuclear cells (PBMCs) and monocytes were stimulated with bovine herpesvirus 1 (BHV-1), LPS and concanavalin A (Con A) to produce L-arginine-dependent nitric oxide (NO) in vitro. NO was detected as early as 12 hrs and up to 72 hrs post stimulation (p.s.). The NO from lipopolysacharide (LPS)-stimulated PBMCs and monocytes was found to exhibit antiviral effect against BHV-1. The anti-BHV-1 effect was inhibited with N(omega)-methyl L-arginine indicating involvement of inducible NO synthase (iNOS) in NO production.
authors.affiliation: National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, UP. ivri@x400.nicgw.nic.in
authors.forename: P K
authors.initials: PK
authors.lastname: Gupta
authors.forename: M
authors.initials: M
authors.lastname: Saini
authors.forename: L K
authors.initials: LK
authors.lastname: Gupta
authors.forename: S K
authors.initials: SK
authors.lastname: Garg
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D008070:Lipopolysaccharides; D009000:Monocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology; D009569:Nitric Oxide / Q000096:biosynthesis*
chemical_list: D008070:Lipopolysaccharides; D009569:Nitric Oxide