The role of cytotoxic necrotizing factor-1 in colonization and tissue injury in a murine model of urinary tract infection.
Cytotoxic necrotizing factor-1 (CNF1) is commonly found in Escherichia coli isolates from patients with urinary tract infection (UTI). To determine whether CNF1 is an important UTI virulence factor we compared the ability of a clinical E. coli UTI isolate and a CNF1-negative mutant of that isolate to colonize and induce histological changes in the urinary tract in a murine model of ascending UTI. We found no evidence that the mutant strain was attenuated.
Johnson
D E
DE
Research Service (151), Department of Veterans Affairs, 10 North Greene St., Baltimore, MD 21201, USA. dejohnso@umaryland.edu
Drachenberg
C
C
Lockatell
C V
CV
Island
M D
MD
Warren
J W
JW
Donnenberg
M S
MS
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D003603:Cytotoxins / Q000378:metabolism*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000254:growth & development* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology; D029968:Escherichia coli Proteins; D005260:Female; D006801:Humans; D051379:Mice; D008808:Mice, Inbred CBA; D014551:Urinary Tract / Q000382:microbiology / Q000473:pathology; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000473:pathology
D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; C060296:cytotoxic necrotizing factor type 1