title: Renal expression of monocyte chemotactic protein-1 and epidermal growth factor in children with obstructive hydronephrosis.
abstract: OBJECTIVE
  The authors studied the potential role of ureteropelvic junction obstruction (UPJ-O) in causing progressive renal damage in children through the renal expression of epidermal growth factor (EGF) and monocyte chemotactic protein-1 (MCP-1) mRNA.
  
  
  METHODS
  Renal tissues were harvested from 11 children with UPJ-O and from 10 normal kidneys to study the renal expression of EGF and MCP-1 detected by means of in situ hybridization. Five of the patients were found to have a history of urinary tract infection (UTI).
  
  
  RESULTS
  Children with UPJ-O had marked reduction of EGF gene expression when compared with controls. Interstitial expression of MCP-1 mRNA was present in all UPJ-O cases. Both EGF and MCP-1 expression did not correlate with age, with differential renal function, and with renal thickness measured through MAG3 renal scan. Children with a history of UTI had a more severe reduction of the renal thickness of the affected kidney compared with those without UTI. MCP-1 expression was higher and EGF more reduced in children with a history of UTI.
  
  
  CONCLUSIONS
  Our results suggest a potential role of EGF and MCP-1 in the pathogenesis of renal damage and growth failure in UPJ-O, especially in children with UTI. These important functional changes begin early in life, possibly during fetal life.
authors.affiliation: Cattedra di Chirurgia Pediatrica, Dipartimento di Biomedicina dell'Età Evolutiva, Università di Bari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bartoli
authors.forename: L
authors.initials: L
authors.lastname: Gesualdo
authors.forename: G
authors.initials: G
authors.lastname: Paradies
authors.forename: E
authors.initials: E
authors.lastname: Caldarulo
authors.forename: B
authors.initials: B
authors.lastname: Infante
authors.forename: G
authors.initials: G
authors.lastname: Grandaliano
authors.forename: R
authors.initials: R
authors.lastname: Monno
authors.forename: S
authors.initials: S
authors.lastname: Leggio
authors.forename: F
authors.initials: F
authors.lastname: Salzillo
authors.forename: F P
authors.initials: FP
authors.lastname: Schena
authors.forename: A
authors.initials: A
authors.lastname: Leggio
mesh_terms: D000293:Adolescent; D001706:Biopsy; D018932:Chemokine CCL2 / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004815:Epidermal Growth Factor / Q000378:metabolism*; D006801:Humans; D006869:Hydronephrosis / Q000378:metabolism*; D017403:In Situ Hybridization; D007223:Infant; D007231:Infant, Newborn; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018932:Chemokine CCL2; D012333:RNA, Messenger; D004815:Epidermal Growth Factor