FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements.
We combined the techniques of fluorescence in situ hybridization (FISH) and chromosomal microdissection in one experiment (FISH-MD). This novel method permits rapid identification of the composition, origin, and breakpoints of rearranged chromosomes. Rearranged chromosomes are first identified by multicolor-FISH, then the fluorophore-labeled derivative chromosomes are directly isolated by microdissection and reverse painted to identify the breakpoints.
Weimer
J
J
Oncology Laboratory, Gynecology and Obstetrics Clinic, Christian-Albrechts University Kiel, Germany. Joerg.Weimer@t-online.de
Kiechle
M
M
Arnold
N
N
D002869:Chromosome Aberrations / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods; D019457:Chromosome Breakage / Q000235:genetics; D020223:Chromosome Painting / Q000379:methods*; D003116:Color; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000378:metabolism; D007621:Karyotyping / Q000379:methods; D008027:Light; D008214:Lymphocytes; D018200:Mixed Tumor, Mullerian / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics; D020161:Physical Chromosome Mapping / Q000379:methods*; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic / Q000235:genetics
D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI