title: beta-adrenoceptor-stimulated lipolysis of subcutaneous abdominal adipocytes as a determinant of fat oxidation in obese men.
abstract: BACKGROUND
  To verify whether an impaired lipolytic capacity of subcutaneous adipocytes may contribute to low rate of fat oxidation.
  
  
  METHODS
  Relationships between adipose tissue lipolysis of subcutaneous (subc) abdominal and femoral isolated adipocytes and respiratory quotient (RQ) were investigated in 20 obese men (age: 44 +/- 5 years; means +/- SD) studied in a fasting state.
  
  
  RESULTS
  Maximal isoproterenol-induced lipolysis was greater in subcutaneous abdominal than in femoral fat cells even if glycerol release was corrected for variation in cell surface area (P < 0.01). On the other hand, no regional variation was observed in the adipose cell lipolytic responses to postadrenoceptor agents such as dibutyryl-cyclic AMP, forskolin and theophylline. Maximal isoproterenol-induced lipolysis of subc abdominal, but not of femoral adipocytes, was inversely related to RQ (r = - 0.61; P < 0. 01) and positively associated to fat oxidation (r = 0.57; P < 0.01). These relationships were independent of possible confounding factors such as fat mass, fat-free mass, waist girth and subc abdominal adipose tissue accumulation assessed by computed tomography, as maximal isoproterenol-induced lipolysis of subcutaneous abdominal adipocytes was the only variable retained as a significant predictor of RQ levels (38% of variance) and of fat oxidation (30% of variance).
  
  
  CONCLUSIONS
  These results suggest that adipose tissue lipolytic activity of subc abdominal adipocytes acts as a determinant of fat oxidation in obese men.
authors.affiliation: Laval University; Laval Hospital; CHUQ Medical Research Center, Québec, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Imbeault
authors.forename: A
authors.initials: A
authors.lastname: Tremblay
authors.forename: J
authors.initials: J
authors.lastname: Després
authors.forename: P
authors.initials: P
authors.lastname: Mauriège
mesh_terms: D000005:Abdomen; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000328:Adult; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D012044:Regression Analysis; D012867:Skin; D013806:Theophylline / Q000494:pharmacology; D044465:White People
chemical_list: D000318:Adrenergic beta-Agonists; D005576:Colforsin; D003994:Bucladesine; D013806:Theophylline; D007545:Isoproterenol