title: Kinetics of appearance of hemorphins from bovine hemoglobin peptic hydrolysates by a direct coupling of reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry.
abstract: Reversed-phase high-performance liquid chromatography coupled with electrospray ionization mass spectrometry was used to improve the preparation of three opioid peptides (Leu-Val-Val-hemorphin-7, Val-Val-hemorphin-7 and Val-Val-hemorphin-4) resulting from bovine hemoglobin peptic hydrolysates. Optimal conditions for the preparation of these peptides were determined thanks to their kinetic studies of appearance in the course of peptic hydrolyses as a function of degree of hydrolysis of hemoglobin. We propose a low degree of hydrolysis (3%) to prepare Leu-Val-Val-hemorphin-7, a mean degree of hydrolysis (11%) to prepare Val-Val-hemorphin-7 and a high degree of hydrolysis (21%) to prepare Val-Val-hemorphin-4.
authors.affiliation: Laboratoire de Technologie des Substances Naturelles, IUT A Lille 1, Villeneuve d'Ascq, France.
authors.forename: R
authors.initials: R
authors.lastname: Froidevaux
authors.forename: B
authors.initials: B
authors.lastname: Lignot
authors.forename: N
authors.initials: N
authors.lastname: Nedjar-Arroume
authors.forename: D
authors.initials: D
authors.lastname: Guillochon
authors.forename: B
authors.initials: B
authors.lastname: Coddeville
authors.forename: G
authors.initials: G
authors.lastname: Ricart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D013058:Mass Spectrometry / Q000379:methods*; D018847:Opioid Peptides / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; D018847:Opioid Peptides