In vitro growth of TS/A adenocarcinoma and of the gene transfected TS/A-IL4 line on biological substrates.
The growth capacity and adaptation of TS/A and TS/A-IL4 lines on laminin, fibronectin, collagens I and IV and matrigel compared to plastics were studied by flow cytometry. On plastic plates, TS/A-IL4 grows in vitro more slowly than the TS/A line and shows a more differentiated phenotype. TS/A-IL4 cells loose the capacity to bind lymphocytes and peroxidase positive cells obtained from mice implanted with the same tumour. The ratio between fibroblast- and epithelial-like cells of TS/A adenocarcinoma is subjected to marked modifications depending on the substrate on which the two cell lines are grown. IL4 release per cell unit is increased by collagen I as is the number of CD54 positive cells, suggesting that, at least in part, the in vivo rejection of TS/A-IL4 tumor might be ascribed to the stimulatory effect of the tissue on the IL4 release by tumor cells. The overall result is that gene modified TS/A-IL4 line shows marked changes of behaviour, most of them depending on the substrate on which tumor cells are growing.
Pacor
S
S
Department of Biomedical Sciences, University of Trieste, Italy.
Magnarin
M
M
Carotenuto
M E
ME
Spessotto
P
P
Zabucchi
G
G
Sava
G
G
D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002453:Cell Cycle; D002454:Cell Differentiation; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D018920:Coculture Techniques; D003094:Collagen; D004338:Drug Combinations; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D005353:Fibronectins; D006084:Graft Rejection; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D007797:Laminin; D008213:Lymphocyte Activation; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000032:analysis; D009368:Neoplasm Transplantation; D010969:Plastics; D011509:Proteoglycans; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D004338:Drug Combinations; D005353:Fibronectins; D007797:Laminin; D009363:Neoplasm Proteins; D010969:Plastics; D011509:Proteoglycans; D011993:Recombinant Fusion Proteins; C059485:matrigel; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D003094:Collagen