Reduction of postischemic contractile dysfunction of the isolated rat heart by sevoflurane: comparison with halothane.
Our aims were to evaluate the effect of sevoflurane on postcardioplegic functional recovery of the isolated rat heart including the role of the adenosine triphosphate regulated potassium (K(ATP)) channels and to compare the cardioprotective effects of equipotent concentrations of halothane and sevoflurane. Isolated perfused rat hearts were subjected to 45 or 60 min normothermic cardioplegic arrest and 30 min reperfusion. Sevoflurane (0.9% and 1. 7%), halothane (0.4% and 0.8%), or sevoflurane (0.9%) plus glibenclamide (10 microM) (a K(ATP) channel blocker) were administered at different time intervals. Measurements of mechanical activity were made before and after arrest. Function during reperfusion after cardioplegic arrest was significantly depressed in both untreated and treated hearts. However, sevoflurane administered both before and after arrest, or before only, significantly improved functional recovery after 45 min of cardioplegia. This protective effect was abolished by simultaneous administration of glibenclamide, suggesting a role of the K(ATP) channel. Sevoflurane was as effective as halothane in improving postcardioplegic functional performance. After 45 min of arrest, hearts exposed to either anesthetic at both concentrations had a significantly higher work performance on discontinuation of their administration than untreated controls. After 60 min of arrest, neither anesthetic elicited protection.
Coetzee
J F
JF
Department of Anesthesiology, University of Stellenbosch Faculty of Medicine, Tygerberg, Republic of South Africa.
le Roux
P J
PJ
Genade
S
S
Lochner
A
A
D000227:Adenine Nucleotides / Q000378:metabolism; D018685:Anesthetics, Inhalation / Q000008:administration & dosage*; D000818:Animals; D005905:Glyburide / Q000008:administration & dosage; D006221:Halothane / Q000008:administration & dosage*; D006324:Heart Arrest, Induced; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008738:Methyl Ethers / Q000008:administration & dosage*; D009200:Myocardial Contraction / Q000187:drug effects*; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane
D000227:Adenine Nucleotides; D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane; D005905:Glyburide; D006221:Halothane