Extracorporeal plateletpheresis induces the interaction of activated platelets with white blood cells.
OBJECTIVE
In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells.


METHODS
Analysis of platelet-leukocyte interaction was performed using multiparameter, three-color flow cytometry.


RESULTS
Over the duration of the procedure, there was an increase in the surface expression of CD62p (P-selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly.


CONCLUSIONS
This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.
Gutensohn
K
K
Transfusion Medicine/Transplantation Immunology, University Hospital Eppendorf, University of Hamburg, Hamburg, Germany. gutensohn@uke.uni-hamburg.de
Alisch
A
A
Krueger
W
W
Kroeger
N
N
Kuehnl
P
P
D000328:Adult; D015703:Antigens, CD / Q000097:blood; D018941:Antigens, CD19 / Q000097:blood; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D017252:CD3 Complex / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood; D002450:Cell Communication; D005112:Extracorporeal Circulation / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000166:cytology; D019007:P-Selectin / Q000096:biosynthesis / Q000097:blood; D015539:Platelet Activation; D010980:Platelet Membrane Glycoproteins; D010983:Plateletpheresis; D013601:T-Lymphocytes / Q000276:immunology; D060149:Tetraspanin 30
D015703:Antigens, CD; D018941:Antigens, CD19; D017252:CD3 Complex; C554586:CD63 protein, human; D015815:Cell Adhesion Molecules; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30