title: Ultrastructural localization of cytochrome c in apoptosis demonstrates mitochondrial heterogeneity.
abstract: Release of apoptogenic factors into the cytosol including cytochrome c is triggering the execution phase of apoptosis through activation of cytoplasmic effector caspases. How loss of function of the electron transport chain can be reconciled with an adequate energy supply necessary for executing the apoptotic program was studied in granulosa cell (GC) sheets cultured up to 72 h without gonadotrophic support. Cytochrome c was localized ultrastructurally by oxidation of diaminobenzidine tetrahydrochloride both in living and fixed cells. In uncultured GC sheets all cells show staining over their entire mitochondrial population. In 72 h cultured sheets in the absence of FSH pre-apoptotic GC's display two subsets of mitochondria: normal sized stained mitochondria and small orthodox mitochondria without demonstrable cytochrome function. Apoptotic cells contain several mitochondria with preservation of respiratory function besides unstained orthodox mitochondria. The cytochrome c containing mitochondria typically display dilated intracristal spaces, a mitochondrial conformation related to increased ATP production. Cytochrome c release was confirmed by Western blotting. In 72 h cultures supplemented with FSH, GC's displayed staining over their entire mitochondrial population. In cultures lacking FSH, but partially protected from apoptosis through caspase inhibition, the cytochrome c release was not inhibited. Thus in the present studied model dysfunction of only a subset of mitochondria is instrumental to initiate the apoptotic program while a functional electron transport chain is maintained until the degradation phase in a subset of respiring mitochondria.
authors.affiliation: Department of Human Anatomy, Embryology & Histology, University of Ghent, Belgium. katharina.dherde@rug.ac.be
authors.forename: K
authors.initials: K
authors.lastname: D'Herde
authors.forename: B
authors.initials: B
authors.lastname: De Prest
authors.forename: S
authors.initials: S
authors.lastname: Mussche
authors.forename: P
authors.initials: P
authors.lastname: Schotte
authors.forename: R
authors.initials: R
authors.lastname: Beyaert
authors.forename: R V
authors.initials: RV
authors.lastname: Coster
authors.forename: F
authors.initials: F
authors.lastname: Roels
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D003370:Coturnix; D003574:Cytochrome c Group / Q000032:analysis / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D003574:Cytochrome c Group; D003576:Electron Transport Complex IV