title: [Physiology of microbial cell and metabolic engineering].
abstract: This review is devoted to the problems of the physiology and cell biology of microorganisms in relation to metabolic engineering. The latter is considered as a branch of fundamental and applied biotechnology aimed at controlling microbial metabolism by methods of genetic engineering and classical genetics and based on intimate knowledge of cell metabolism. Attention is also given to the problems associated with the metabolic limitation of microbial biosyntheses, analysis and control of metabolic fluxes, rigidity of metabolic pathways, the role of pleiotropic (global) regulatory systems in the control of metabolic fluxes, and prospects of physiological and evolutionary approaches in metabolic engineering.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow oblast, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Golovlev
authors.forename: L A
authors.initials: LA
authors.lastname: Golovleva
mesh_terms: D005818:Genetic Engineering; D008828:Microbiological Techniques; D008829:Microbiology