title: Detection of peptidoglycan in human plasma using the silkworm larvae plasma test.
abstract: Silkworm larvae plasma (SLP) reagent, which is prepared from the body fluid of the silkworm, reacts with peptidoglycan (PG), a fragment of both the Gram-positive and Gram-negative bacterial cell wall, as well as with beta-glucan, a component of fungi. We developed a quantitative method for the detection of PG in human plasma from cases with bacterial infection using the SLP reagent. Tested in this way, the SLP method showed 86.2% sensitivity, 90.6% specificity, 89.3% positive predictive value, and 88.5% efficiency. The SLP method provides a valuable tool for the diagnosis of systemic infection using patients' blood.
authors.affiliation: The First Department of Surgery, Shiga University of Medical Science, Seta Tsukinowa, Otsu, Shiga, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Tani
authors.forename: T
authors.initials: T
authors.lastname: Yokota
authors.forename: M
authors.initials: M
authors.lastname: Kodama
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology; D001419:Bacteria / Q000737:chemistry; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D001681:Biological Assay; D012831:Bombyx; D002473:Cell Wall / Q000737:chemistry; D006801:Humans; D007814:Larva; D010457:Peptidoglycan / Q000097:blood*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D010457:Peptidoglycan