HIV-1 subtyping in Salvador, Bahia, Brazil: a city with African sociodemographic characteristics.
To investigate the prevalence of the HIV-1 subtypes in different populations from Salvador, Bahia, Brazil, blood samples from 72 HIV-1-seropositive injecting drug users (IDUs) and 62 individuals infected sexually were analyzed using the heteroduplex mobility assay (HMA). In the IDU group, 89.5% were classified as subtype B, 3% as subtype F, and 7.5% showed a B/F HMA profile. In the sexual transmission (ST) group, 95% were identified as B subtype, 3.4% showed a B/F profile, and 1.6% a B/C/E HMA profile. All Brazilian samples that showed multiple reactivities in the HMA analysis clustered on sequencing with B North American/ European HIV-1 isolates in the phylogenetic analysis, whereas the F subtypes clustered with F Brazilian HIV-I isolates. Serologic reactivities of IDU's sera were examined using a panel of synthetic V3 loop peptides representative of the different HIV-1 subtypes. No difference in serologic reactivity between F and B subtype plasma could be observed. Predominance of HIV-I subtype B was identified in both study groups, whereas subtype F was detected only among IDUs in a frequency lower than described for other Brazilian regions.
Couto-Fernandez
J C
JC
Advanced Laboratory of Public Health-LASP, Gonçalo Moniz Research Center-CPqGM, Oswaldo Cruz Foundation, UNAIDS Collaborating Center, Salvador, Bahia. coutofer@gene.dbbm.fiocruz.br
Morgado
M G
MG
Bongertz
V
V
Tanuri
A
A
Andrade
T
T
Brites
C
C
Galvão-Castro
B
B
D000293:Adolescent; D000328:Adult; D000349:Africa / Q000208:ethnology; D000940:Antigenic Variation; D044383:Black People; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015658:HIV Infections / Q000276:immunology / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000276:immunology; D020180:Heteroduplex Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012725:Sexual Behavior; D012959:Socioeconomic Factors; D015819:Substance Abuse, Intravenous / Q000150:complications