A sensitive assay for anti-HIV-1 drug discovery in a biological safety level-2 laboratory.
Studies involving infectious, wild type HIV-1 must be performed under strict BSL-3 practice. We have employed a defective (deltaTat/Rev)MC99 and cloned 1A2 line, ie, mutated HIV-1 and Tat/Rev transfected cells to verify anti-HIV-1 activity in a BSL-2 laboratory. A number of extracts from various parts of 11 species of plants were studied. Results were correlated with those of an anti-HIV-1 reverse transcriptase (RT) assay.
Yoosook
C
C
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Reutrakul
V
V
Santisuk
T
T
Chaichana
S
S
Pezzuto
J M
JM
Dong
Y
Y
Clanton
D J
DJ
Kiser
R
R
Staley
P
P
D019380:Anti-HIV Agents; D001681:Biological Assay / Q000379:methods*; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D015195:Drug Design; D015726:Giant Cells / Q000187:drug effects / Q000378:metabolism; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors*; D006801:Humans; D007757:Laboratory Infection / Q000517:prevention & control; D010936:Plant Extracts / Q000627:therapeutic use; D018894:Reverse Transcriptase Inhibitors
D019380:Anti-HIV Agents; D010936:Plant Extracts; D018894:Reverse Transcriptase Inhibitors; D054303:HIV Reverse Transcriptase