Total free radical trapping antioxidant potential in pre-eclampsia.
OBJECTIVE
The aim of the present study was to measure serum urate, tocopherol and ascorbate and to calculate total radical trapping antioxidant potential (TRAP) in pre-eclampsia.


METHODS
Study samples were taken from 25 pre-eclamptic, 25 normotensive pregnant and 25 non-pregnant healthy females for measuring uric acid, vitamin C and E, total sulfhydryl group. Also lipid peroxidation product malonaldehyde (MDA), the superoxide generation (NBT reduction) were estimated and TRAP was calculated. Data were analyzed statistically using Student's t-test.


RESULTS
Pre-eclamptic women had significantly increased MDA levels, superoxide generation and uric acid levels as compared to control subjects (P<0.001). Vitamin C and E and total thiols were lower in pre-eclampsia as compared to control subjects (P<0.001). TRAP was significantly higher in pre-eclampsia.


CONCLUSIONS
TRAP provides a means of assessing the total capacity of the chain-breaking antioxidants to prevent lipid peroxidation in plasma.
Kharb
S
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Department of Biochemistry, Pt. B.D. Sharma PGIMS, Rohtak, India.
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood; D015415:Biomarkers / Q000097:blood; D005260:Female; D005609:Free Radicals / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood* / Q000503:physiopathology; D011237:Predictive Value of Tests; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D014527:Uric Acid / Q000097:blood; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D015415:Biomarkers; D005609:Free Radicals; D014810:Vitamin E; D014527:Uric Acid; D008315:Malondialdehyde; D001205:Ascorbic Acid