Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B, from the chicken duodenum.
The ability of Lactobacillus rhamnosus strains GG and LC-705 to remove AFB1 from the intestinal luminal liquid medium has been tested in vivo using a chicken intestinal loop technique. In this study, the GG strain of L. rhamnosus decreased AFB1 concentration by 54% in the soluble fraction of the luminal fluid within 1 min. This strain was more efficient in binding AFB1 compared with L. rhamnosus strain LC-705 (P < 0.05) that removed 44% of AFBl under similar conditions. Accumulation of AFB1 into the intestinal tissue was also determined. There was a 74% reduction in the uptake of AFB1 by the intestinal tissue, in the presence of L. rhamnosus strain GG compared with 63% and 37% in the case of Propionibacterium freudenreichii ssp. shermanii JS and L. rhamnosus strain LC-705, respectively. The complexes formed in vitro between either L. rhamnosus strain GG or L. rhamnosus strain LC-705 and AFB1 were stable under the luminal conditions for a period of 1 h.
El-Nezami
H
H
Key Centre for Applied and Nutritional Toxicology, RMIT-University, Melbourne, Vic, Australia.
Mykkänen
H
H
Kankaanpää
P
P
Salminen
S
S
Ahokas
J
J
D016604:Aflatoxin B1 / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000378:metabolism / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid; D004386:Duodenum / Q000378:metabolism / Q000382:microbiology*; D007778:Lactobacillus / Q000378:metabolism*; D011424:Propionibacterium / Q000378:metabolism*
D016604:Aflatoxin B1