A facile synthesis of DNA oligomers with modified backbones via the phosphoramidite method without nucleoside base protection.
A convenient synthesis of DNA oligomers with modified back bones including phosphorothioates and phosphotriesters has been developed on the basis of the phosphoramidite strategy without nucleoside base protection. In this synthesis, a new, convenient method for the preparation of N-free-5'-O-dimethoxytrityl-2'-deoxyribonucleoside by the direct O-selective dimethoxytritylation of the parent nucleosides has also been disclosed.
Kataoka
M
M
Graduate School of Human Informatics, Nagoya University, Chikusa, Japan.
Kawai
R
R
Hayakawa
Y
Y
D000577:Amides; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D010756:Phosphoric Acids; D013873:Thionucleotides
D000577:Amides; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D010756:Phosphoric Acids; D013873:Thionucleotides; D004247:DNA; C011067:phosphoramidic acid