title: Reliability of immunohistochemical demonstration of oestrogen receptors in routine practice: interlaboratory variance in the sensitivity of detection and evaluation of scoring systems.
abstract: OBJECTIVE
  To investigate interlaboratory variance in the immunohistochemical (IHC) detection of oestrogen receptors so as to determine the rate of false negatives, which could adversely influence the decision to give adjuvant tamoxifen treatment.
  
  
  METHODS
  To ensure that similar results are obtained by different institutions, 200 laboratories from 26 countries have joined the UK national external quality assessment scheme for immunocytochemistry (NEQAS-ICC). Histological sections from breast cancers having low, medium, and high levels of oestrogen receptor expression were sent to each of the laboratories for immunohistochemical staining. The results obtained were evaluated for the sensitivity of detection, first by estimating threshold values of 1% and 10% of stained tumour cells, and second by the Quick score method, by a panel of four assessors judging individual sections independently on a single blind basis. The results were also evaluated using participants' own threshold values.
  
  
  RESULTS
  Over 80% of laboratories were able to demonstrate oestrogen receptor positivity on the medium and high expressing tumours, but only 37% of laboratories scored adequately on the low expressing tumour. Approximately one third of laboratories failed to register any positive staining in this tumour, while one third showed only minimal positivity.
  
  
  CONCLUSIONS
  There is considerable interlaboratory variability, especially in relation to the detection of breast cancers with low oestrogen receptor positivity, with a false negative rate of between 30% and 60%. This variability appears to be caused by minor differences in methodology that may be rectified by fine adjustment of overall technique.
authors.affiliation: Department of Histopathology, University College London Medical School, UK. rmkdhcr@ucl.ac.uk
authors.forename: A
authors.initials: A
authors.lastname: Rhodes
authors.forename: B
authors.initials: B
authors.lastname: Jasani
authors.forename: D M
authors.initials: DM
authors.lastname: Barnes
authors.forename: L G
authors.initials: LG
authors.lastname: Bobrow
authors.forename: K D
authors.initials: KD
authors.lastname: Miller
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry*; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007753:Laboratories / Q000592:standards*; D009363:Neoplasm Proteins / Q000032:analysis*; D011785:Quality Assurance, Health Care; D011960:Receptors, Estrogen / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016037:Single-Blind Method
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D011960:Receptors, Estrogen