Silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
The effect of silkworm hemolymph on baculovirus-induced insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity; however, its addition after the infection was less effective. This can be explained by the higher transfer rate of silkworm hemolymph which is caused by endocytosis during the virus internalization step. The delayed cell death due to silkworm hemolymph was not caused by an inhibition of the virus attachment and internalization steps. The apoptosis was analyzed using DNA fragmentation and TUNEL assays, and the resulting data confirm that silkworm hemolymph inhibits baculovirus-induced insect cell apoptosis.
Rhee
W J
WJ
School of Chemical Engineering, Seoul National University, Kwanak-Gu Shilim-Dong San 56-1, Seoul, 151-742, Korea.
Park
T H
TH
D000818:Animals; D017209:Apoptosis; D016367:Baculoviridae / Q000378:metabolism*; D012831:Bombyx / Q000378:metabolism*; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression / Q000187:drug effects; D006458:Hemolymph / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D011994:Recombinant Proteins; D001616:beta-Galactosidase