title: The stability and maturation of the H2A histone mRNAs from Trypanosoma cruzi are implicated in their post-transcriptional regulation.
abstract: We have recently described that the Trypanosoma cruzi histone H2A genes are actively transcribed as two sized classes of polyadenylated transcripts and that they differ in the 3'-UTRs due to the insertion of a partial SINE sequence in the 3'-end of some of H2A gene units. The expression of the H2A genes in the non-replicative trypomastigote forms is very low, whereas in the replicative forms, there is significant and constitutive transcription of the H2A genes probably regulated in a posttranscriptional way and associated to DNA replication. The data presented in this paper reveal that in epimastigotes, the steady-state levels of the H2A mRNAs are determined by controlling the stability of the messengers in the cytoplasm, most likely mediated by a nuclease attack. The data also indicate that there must be an additional control, associated to the parasite growth phase, which may act at the maturation step of the transcripts. The data suggest, moreover; that the cytoplasmic level of the H2A protein might be involved in the regulation of its own synthesis by controlling translation of existing messengers.
authors.affiliation: Instituto de Parasitología y Biomedicina López Neyra Consejo Superior de Investigaciones Científicas, Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Marañón
authors.forename: M C
authors.initials: MC
authors.lastname: Thomas
authors.forename: C
authors.initials: C
authors.lastname: Puerta
authors.forename: C
authors.initials: C
authors.lastname: Alonso
authors.forename: M C
authors.initials: MC
authors.lastname: López
mesh_terms: D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D003593:Cytoplasm / Q000737:chemistry; D017125:Genes, Protozoan; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D006918:Hydroxyurea / Q000494:pharmacology; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry*; D013997:Time Factors; D014158:Transcription, Genetic; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
chemical_list: D006657:Histones; D012333:RNA, Messenger; D016590:Aphidicolin; D006918:Hydroxyurea