Recognition control of the nucleic acid model through conformational switching of nucleobase induced by borate ester formation of cis-2',3'-diol.
A novel nucleic acid model that possessed 5'-amino-5'-deoxyuridine at alpha- and gamma-position of L-glutamic acid through amide linkage using 5'-amino group was synthesized and the conformation and the hybridization properties were studied. The complex of alpha-PRNA with complementary DNA/RNA was more stable than the corresponding natural duplex in the absence of borate. Its recognition ability was however lost when borax was added to the solution.
Wada
T
T
Faculty of Engineering, Osaka University, Suita, Japan.
Minamimoto
N
N
Satoh
H
H
Inoue
Y
Y
D001881:Borates; D004247:DNA / Q000737:chemistry*; D003857:Deoxyuridine / Q000031:analogs & derivatives; D018698:Glutamic Acid; D007202:Indicators and Reagents; D007700:Kinetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
C113077:5'-amino-5'-deoxyuridine; D001881:Borates; D007202:Indicators and Reagents; D020135:Peptide Nucleic Acids; D018698:Glutamic Acid; D012313:RNA; D004247:DNA; C010634:sodium borate; D003857:Deoxyuridine