Effect of tumor-conditioned medium on intercellular communication and proliferation of Balb/c 3T3 cells.
The possible role of tumor cell-derived factors in the regulation of gap junctional intercellular communication and proliferation of fibroblasts was studied in a model system of Balb/c 3T3 cells growing in tumor conditioned medium by Lucifer Yellow CH dye-transfer and BrdU incorporation assays. Six to 24 h incubation of Balb/c 3T3 cells in a medium conditioned by WiDr adenocarcinoma cells enhanced the gap junctional communication between the cells by 25-40% as revealed by intercellular transfer of a fluorescent dye Lucifer Yellow CH. Simultaneously the cell proliferation rates were examined and found to be reduced by 23% at 24 h treatment. Since adenocarcinoma cells are known to secrete different growth factor-like polypeptides into their conditioned medium, we suppose that tumors that produce these molecules might alter their host environment through the enhancement of cell-cell communication thereby facilitating the exchange of modulatory factors.
Tóth
L
L
Department of Hygiene and Epidemiology, University Medical School of Debrecen, Hungary.
Pásti
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G
Sárváry
A
A
Balázs
M
M
Adány
R
R
D016475:3T3 Cells; D000230:Adenocarcinoma; D000818:Animals; D001973:Bromodeoxyuridine; D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D003110:Colonic Neoplasms; D017077:Culture Media, Conditioned; D005456:Fluorescent Dyes; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D007546:Isoquinolines; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow; D001973:Bromodeoxyuridine