Developmental and lesion-induced changes in the distribution of the glucose transporter Glut-1 in the central and peripheral nervous system.
The active transport of D-glucose from the vascular to the neural compartment requires the presence of a carrier molecule at the blood-brain and the blood-nerve barrier. The glucose transporter 1 (Glut-1) seems to be the main carrier in blood-tissue barriers of endothelial and perineurial type. The distribution of Glut-1 was assessed in the normal central and peripheral nervous system of young and adult animals and compared with changes after nerve injury. Immuno-histochemistry, in situ hybridization, and perfusions with Evans Blue were carried out. Glut- I was not expressed in the perineurium of peripheral nerves at birth, but appeared in the perineurium of peripheral nerves, spinal roots, in the capsule of dorsal-root ganglia, and in the pia mater of adult animals. The perineurium of peripheral nerves subjected to Wallerian degeneration presented a faint Glut-1 immunoreaction, which was restored after regeneration. Glut-1 was expressed in capillaries of the gray substance of the spinal cord. Perineurial-derived lamellar cells of Pacinian corpuscles exhibited a strong Glut-1-like immunoreactivity in response to denervation and during development. Merkel cells and Meissner corpuscles were found to be Glut-1 negative. Glut-1 seems to reflect the quality of an adult, mature perineurial and blood-nerve barrier.
Stark
B
B
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. Birgit.Stark@neuro.ki.se
Carlstedt
T
T
Cullheim
S
S
Risling
M
M
D000328:Adult; D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology / Q000293:injuries / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D051272:Glucose Transporter Type 1; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism; D010141:Pacinian Corpuscles / Q000378:metabolism; D010525:Peripheral Nerves / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000166:cytology / Q000293:injuries / Q000378:metabolism*; D013116:Spinal Cord / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D013126:Spinal Nerve Roots / Q000166:cytology / Q000293:injuries / Q000378:metabolism
D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495677:SLC2A1 protein, human; C495679:Slc2a1 protein, rat