title: Different effects of dominant negative mutants of desmocollin and desmoglein on the cell-cell adhesion of keratinocytes.
abstract: Desmosomes contain two types of cadherin: desmocollin (Dsc) and desmoglein (Dsg). In this study, we examined the different roles that Dsc and Dsg play in the formation of desmosomes, by using dominant-negative mutants. We constructed recombinant adenoviruses (Ad) containing truncated mutants of E-cadherin, desmocollin 3a, and desmoglein 3 lacking a large part of their extracellular domains (EcaddeltaEC, Dsc3adeltaEC, Dsg3deltaEC), using the Cre-loxP Ad system to circumvent the problem of the toxicity of the mutants to virus-producing cells. When Dsc3adeltaEC Ad-infected HaCaT cells were cultured with high levels of calcium, E-cadherin and beta-catenin, which are marker molecules for the adherens junction, disappeared from the cell-cell contact sites, and cell-cell adhesion was disrupted. This also occurred in the cells infected with EcaddeltaEC Ad. With Dsg3deltaEC Ad infection, keratin insertion at the cell-cell contact sites was inhibited and desmoplakin, a marker of desmosomes, was stained in perinuclear dots while the adherens junctions remained intact. Dsc3adeltaEC Ad inhibited the induction of adherens junctions and the subsequent formation of desmosomes with the calcium shift, while Dsg3deltaEC Ad only inhibited the formation of desmosomes. To further determine whether Dsc3adeltaEC directly affected adherens junctions, mouse fibroblast L cells transfected with E-cadherin (LEC5) were infected with these mutant Ads. Both Dsc3adeltaEC and EcaddeltaEC inhibited the cell-cell adhesion of LEC5 cells, as determined by the cell aggregation assay, while Dsg3deltaEC did not. These results indicate that the dominant negative effects of Dsg3deltaEC were restricted to desmosomes, while those of Dsc3adeltaEC were observed in both desmosomes and adherens junctions. Furthermore, the cytoplasmic domain of Dsc3adeltaEC coprecipitated both plakoglobin and beta-catenin in HaCaT cells. In addition, beta-catenin was found to bind the endogenous Dsc in HaCaT cells. These findings lead us to speculate that Dsc interacts with components of the adherens junctions through beta-catenin, and plays a role in nucleating desmosomes after the adherens junctions have been established.
authors.affiliation: Department of Dermatology, School of Medicine, Ehime University, Ehime, Japan. hanakawa@m.ehime-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Hanakawa
authors.forename: M
authors.initials: M
authors.lastname: Amagai
authors.forename: Y
authors.initials: Y
authors.lastname: Shirakata
authors.forename: K
authors.initials: K
authors.lastname: Sayama
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D051187:Desmocollins; D051184:Desmoglein 3; D051182:Desmogleins; D051180:Desmoplakins; D003896:Desmosomes / Q000235:genetics* / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D009154:Mutation / Q000235:genetics*; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary / Q000235:genetics; D019108:Tight Junctions / Q000235:genetics / Q000378:metabolism; D051185:gamma Catenin
chemical_list: D015820:Cadherins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; C495294:DSP protein, human; D051187:Desmocollins; D051184:Desmoglein 3; D051182:Desmogleins; D051180:Desmoplakins; C495300:Dsg3 protein, mouse; C495295:Dsp protein, mouse; D051185:gamma Catenin; D002118:Calcium