Selective cytotoxic activity of grape peel and seed extracts against oral tumor cell lines.
Grape seed extracts were more cytotoxic than grape peel extracts. Methanol and 70% methanol extracts of grape seed selectively killed two human oral tumor cell lines, more efficiently than human gingival fibroblasts. ESR spectroscopy revealed that these extracts produced radicals under alkaline conditions and enhanced the radical intensity of sodium ascorbate at higher concentrations. On the other hand, lower concentration of these extracts slightly reduced the radical intensity of sodium ascorbate, and scavenged superoxide anion, generated by hypoxanthine and xanthine oxidase reaction. These properties of grape seed extracts suggest their possible application for cancer prevention.
Shirataki
Y
Y
Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Kawase
M
M
Saito
S
S
Kurihara
T
T
Tanaka
W
W
Satoh
K
K
Sakagami
H
H
Motohashi
N
N
D016588:Anticarcinogenic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D001205:Ascorbic Acid / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004578:Electron Spin Resonance Spectroscopy; D005347:Fibroblasts / Q000187:drug effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals; D005881:Gingiva / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D009062:Mouth Neoplasms / Q000473:pathology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D019685:Rosales / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D012997:Solvents; D013481:Superoxides / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016588:Anticarcinogenic Agents; D000972:Antineoplastic Agents, Phytogenic; D016166:Free Radical Scavengers; D005609:Free Radicals; D010936:Plant Extracts; D012997:Solvents; D013481:Superoxides; D001205:Ascorbic Acid; D000432:Methanol