A novel polypeptide p10 expressed in tumor-promoter-treated murine epidermis and in untreated neonatal murine epidermis.
12-O-Tetradecanoyl-phorbol-13-acetate (TPA) has been shown to induce a broad variety of morphological and metabolic changes in mouse skin. These include gross changes in cell growth as well as subtle changes regarding gene expression. Changes in the induction of specific proteins have been described, but it is not yet clear, if there exists a strict causal correlation between the induction of expression of specific proteins and the phenomenon of tumor promotion. At least some of these proteins might also be correlated with an accompanying hyperproliferative effect. This has been shown for protein p10, a 10 kDa protein not found in untreated adult mouse epidermis. The expression of this protein can be induced de novo after the topical administration of TPA. It is also shown, that p10 is expressed in untreated neonatal murine epidermis. Due to the induction of this protein by irritation, hyperproliferation-causing tumor promoters and due to the observable abundance in neonatal epidermis, this protein seems not strictly related to tumor promotion, but rather to proliferation and differentiation.
Schlatterer
K
K
Gustav-Embden Zentrum der Biologischen Chemie, Abt. Molekularbiologie, Johann-Wolfgang-von-Goethe Universität Frankfurt, Germany.
Schlatterer
B
B
Krauter
G
G
Hecker
E
E
Chandra
P
P
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology*; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002273:Carcinogens / Q000494:pharmacology*; D002454:Cell Differentiation; D002455:Cell Division; D004817:Epidermis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007525:Isoelectric Focusing; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D010212:Papilloma / Q000139:chemically induced / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012878:Skin Neoplasms / Q000139:chemically induced / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D009363:Neoplasm Proteins; D011506:Proteins; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate