Effect of hydrocortisone on platelet activating factor induced lung edema in isolated rabbit lungs.
The effect of hydrocortisone on platelet activating factor (PAF)-induced pulmonary edema is studied. Thirty four isolated and perfused rabbit lung preparations were used: eight control preparations, eight PAF preparations with two doses of PAF called low dose (LD = 0.5 microg/kg of rabbit weight) and high dose (HD = 1 microg/kg of rabbit weight). Eighteen preparations divided in three groups of six were pretreated with doses of 20, 200 and 2000 mg of hydrocortisone and later given the same doses of PAF as described above. Hydrocortisone significantly decreased (P < 0.05) the effect of PAF LD over the pulmonary arterial pressure (Ppa) in the 200 and 2000 mg groups (58 and 89% decrease, respectively) and it significantly decreased (P < 0.05) the effect of PAF HD over Ppa in all hydrocortisone pretreated groups (48, 70 and 96% decrease, respectively). Fluid filtration rate (FFR) increases mediated by PAF HD were significantly inhibited (P < 0.05) in the 200 and 2000 mg groups (64 and 96% decrease, respectively). We conclude that hydrocortisone inhibits the effect of PAF over the pulmonary circulation.
Friedman
E
E
Instituto de Medicina Experimental, Facultad de Medicina, Escuela Luis Razetti, Universidad Central de Venezuela, Caracas.
Novoa
E
E
Crespo
A
A
Marcano
H
H
Pesce
L
L
Comellas
A
A
Sánchez de León
R
R
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology*; D066298:In Vitro Techniques; D010972:Platelet Activating Factor; D011175:Positive-Pressure Respiration; D011652:Pulmonary Circulation / Q000187:drug effects; D011654:Pulmonary Edema / Q000139:chemically induced*; D011817:Rabbits
D000893:Anti-Inflammatory Agents; D010972:Platelet Activating Factor; D006854:Hydrocortisone