title: The amino acid sequence of the agglutinin isolated from the red marine alga Bryothamnion triquetrum defines a novel lectin structure.
abstract: The primary structure of a lectin isolated from the red alga Bryothamnion triquetrum was established by combination of Edman degradation of sets of overlapping peptides and mass spectrometry. It contains 91 amino acids and two disulphide bonds. The primary structure of the B. triquetrum lectin does not show amino acid sequence similarity with known plant and animal lectin structures. Hence, this protein may be the paradigm of a novel lectin family.
authors.affiliation: Instituto de Biomedicina de Valencia, Spain. jcalvete@ibv.csic.es
authors.forename: J J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: F H
authors.initials: FH
authors.lastname: Costa
authors.forename: S
authors.initials: S
authors.lastname: Saker-Sampaio
authors.forename: M P
authors.initials: MP
authors.lastname: Murciano
authors.forename: C S
authors.initials: CS
authors.lastname: Nagano
authors.forename: B S
authors.initials: BS
authors.lastname: Cavada
authors.forename: T B
authors.initials: TB
authors.lastname: Grangeiro
authors.forename: M V
authors.initials: MV
authors.lastname: Ramos
authors.forename: C
authors.initials: C
authors.lastname: Bloch
authors.forename: S B
authors.initials: SB
authors.lastname: Silveira
authors.forename: B T
authors.initials: BT
authors.lastname: Freitas
authors.forename: A H
authors.initials: AH
authors.lastname: Sampaio
mesh_terms: D020418:Algal Proteins; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis; D004220:Disulfides / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D037102:Lectins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000461:Rhodophyta / Q000737:chemistry*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020418:Algal Proteins; D004220:Disulfides; D037102:Lectins; D010446:Peptide Fragments; D020033:Protein Isoforms; D005688:Galactosamine; D003545:Cysteine; D005944:Glucosamine