title: Characterization of Ngef, a novel member of the Dbl family of genes expressed predominantly in the caudate nucleus.
abstract: We have identified Ngef as a novel member of the family of Dbl genes. Many members of this family have been shown to function as guanine nucleotide exchange factors for the Rho-type GTPases. Ngef is predominantly expressed in brain, with the strongest signal in the caudate nucleus, a region associated with the control of movement. Ngef contains a translated trinucleotide repeat, a polyglutamic acid stretch interrupted by a glycine. We have localized the Ngef gene to mouse chromosome 1 and the human homologue of Ngef to human chromosome 2q37. We have shown in preliminary experiments that Ngef has transforming potential in cell culture and is able to induce tumors in nude mice.
authors.affiliation: Department of Human Anatomy and Genetics, University of Oxford, South Parks Road, Oxford, OX1 3QX, United Kingdom.
authors.forename: N R
authors.initials: NR
authors.lastname: Rodrigues
authors.forename: A M
authors.initials: AM
authors.lastname: Theodosiou
authors.forename: M A
authors.initials: MA
authors.lastname: Nesbit
authors.forename: L
authors.initials: L
authors.lastname: Campbell
authors.forename: A T
authors.initials: AT
authors.lastname: Tandle
authors.forename: D
authors.initials: D
authors.lastname: Saranath
authors.forename: K E
authors.initials: KE
authors.lastname: Davies
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002421:Caudate Nucleus / Q000378:metabolism*; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D020662:Guanine Nucleotide Exchange Factors; C057811:MCF2 protein, human; C512843:Mcf2 protein, mouse; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger