Metabolic effects of L-carnitine on prepubertal rat Sertoli cells.
The role of carnitine on Sertoli cell metabolism was investigated. Carnitine effects on Sertoli cell lipid metabolism were evaluated by measuring the intracellular levels of non-esterified fatty acids (NEFA) and ketone bodies. The concentration of NEFA in Sertoli cell cultured in the presence of carnitine is significantly reduced as compared to control, while, no significant changes were observed in the concentration of ketone bodies. The functional parameters evaluated to assess the influence of carnitine on Sertoli cell carbohydrate metabolism, i.e., lactate and pyruvate production, lactate dehydrogenase activity and hexose transport, were all significantly increased following carnitine in vitro supplementation. Thus, carnitine appears to drive Sertoli cell intermediary metabolism in an intimately interrelated way, stimulating both fatty acid breakdown and glycolysis. Our results indicate that Sertoli cells are a possible target for a widespread metabolic action of carnitine and strongly support the involvement of carnitine in the regulation of Sertoli cell functions which are related with germ cell "nutrition", convincingly suggesting a direct influence of the compound at testis level.
Palmero
S
S
Dipartimento di Biologia Sperimentale, Ambientale ed Applicata, Universita degli Studi di Genova, Italy. spalmero@csita.unige.it
Bottazzi
C
C
Costa
M
M
Leone
M
M
Fugassa
E
E
D000818:Animals; D001692:Biological Transport; D002331:Carnitine / Q000494:pharmacology*; D002478:Cells, Cultured; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008297:Male; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism*; D012741:Sexual Maturation
D005230:Fatty Acids, Nonesterified; D006601:Hexoses; D007657:Ketone Bodies; D007773:Lactates; D011773:Pyruvates; D002331:Carnitine