Gene trap insertional mutagenesis in mice: new vectors and germ line mutations in two novel genes.
Insertional mutagenesis based on gene trap vectors that capture endogenous splice sites is a promising tool for functional genomics. Several groups have proposed large-scale gene trap screens, but questions remain as to the type of vectors and their design. We report a set of plasmid-encoded gene trap vectors and the disruption of two novel genes. Our results include a comparison of the relative gene trapping efficiencies of two different splice acceptor sequences in ES cells and an analysis of the structure of several gene trap insertions.
Neilan
E G
EG
Department of Pediatrics, Stanford University, Palo Alto, CA 94305, USA.
Barsh
G S
GS
D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation; D015686:Gene Products, env / Q000235:genetics; D005822:Genetic Vectors; D018095:Germ-Line Mutation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000379:methods*; D010641:Phenotype; D012326:RNA Splicing; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D015686:Gene Products, env; D001616:beta-Galactosidase