title: Conformation and local environment of nucleotides bound to HIV type 1 reverse transcriptase (HIV-1 RT) in the ground state.
abstract: Nuclear Overhauser effect experiments were used to characterize the protein environment and conformations of dTTP, dATP and AZTTP bound to HIV-RT in the ground state. The results show the initial binding sites for the nucleotides overlap but are not completely coincident. All of the bound nucleotides assume the same anti C4'-exo conformation.
authors.affiliation: Triangle Pharmaceuticals, Inc. Durham, NC, USA.
authors.forename: G R
authors.initials: GR
authors.lastname: Painter
authors.forename: C W
authors.initials: CW
authors.lastname: Andrews
authors.forename: P A
authors.initials: PA
authors.lastname: Furman
mesh_terms: D019380:Anti-HIV Agents / Q000737:chemistry* / Q000378:metabolism; D001667:Binding, Competitive; D003838:Deoxyadenine Nucleotides / Q000737:chemistry* / Q000378:metabolism; D054306:Dideoxynucleotides; D004591:Electrophoresis, Polyacrylamide Gel; D054303:HIV Reverse Transcriptase / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D013942:Thymine Nucleotides / Q000737:chemistry* / Q000378:metabolism; D015215:Zidovudine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D019380:Anti-HIV Agents; D003838:Deoxyadenine Nucleotides; D054306:Dideoxynucleotides; D013942:Thymine Nucleotides; D015215:Zidovudine; C052873:zidovudine triphosphate; D054303:HIV Reverse Transcriptase; C024157:thymidine 5'-triphosphate