Genotypic analysis of hepatitis C virus in blood donors in Indonesia.
A study was conducted to describe the genetic diversity of hepatitis C virus (HCV) in a population of positive blood donors from throughout Indonesia. Repeat analysis by reverse transcription-polymerase chain reaction (RT-PCR) of 102 anti-HCV positive samples showed that 67 gave HCV-specific positive signals by the PCR for the 5'-untranslated genomic region of HCV. Further genotypic analysis on 64 HCV RNA-positive samples indicated that 57 belonged to the following individual genotypes: 1a, 1b, 2a, 2b, and 3b. The predominant HCV genotypes in this donor population were 1b (57.8%), 2a (17.2%), and 3b (10.9%). The core sequences of the 4 indeterminate samples when aligned with published sequences of various HCV genotypes showed a range of homology from 16.16% to 78.67%. Comparative analysis of genotypic representation from other anti-HCV-positive study populations, including polytransfused pediatric and adult renal dialysis groups, is now being carried out to determine the potential genotypic association with mechanistic HCV spread.
Inoue
Y
Y
Dainabot Co. Ltd., Tokyo, Japan.
Sulaiman
H A
HA
Matsubayashi
K
K
Julitasari
Iinuma
K
K
Ansari
A
A
Laras
K
K
Corwin
A L
AL
D020121:5' Untranslated Regions / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D001782:Blood Donors; D002648:Child; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D016174:Hepacivirus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006526:Hepatitis C / Q000635:transmission* / Q000821:virology; D006801:Humans; D007214:Indonesia; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D020121:5' Untranslated Regions; D017931:DNA Primers; D004279:DNA, Viral; D012367:RNA, Viral