Scorpine, an anti-malaria and anti-bacterial agent purified from scorpion venom.
A novel peptide, scorpine, was isolated from the venom of the scorpion Pandinus imperator, with anti-bacterial activity and a potent inhibitory effect on the ookinete (ED(50) 0.7 microM) and gamete (ED(50) 10 microM) stages of Plasmodium berghei development. It has 75 amino acids, three disulfide bridges with a molecular mass of 8350 Da. Scorpine has a unique amino acid sequence, similar only to some cecropins in its N-terminal segment and to some defensins in its C-terminal region. Its gene was cloned from a cDNA library.
Conde
R
R
Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Avenida Universidad, 2001, P.O. Box 510-3, Cuernavaca, Mexico.
Zamudio
F Z
FZ
Rodríguez
M H
MH
Possani
L D
LD
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000962:Antimalarials / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D023082:Defensins; D004220:Disulfides / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005718:Gametogenesis / Q000187:drug effects; D005854:Germ Cells / Q000187:drug effects; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology; D010962:Plasmodium berghei / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D012604:Scorpion Venoms / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D012605:Scorpions / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D000900:Anti-Bacterial Agents; D000962:Antimalarials; D023181:Antimicrobial Cationic Peptides; D023082:Defensins; D004220:Disulfides; D010455:Peptides; D011506:Proteins; D012604:Scorpion Venoms; C093645:Shiva 3; C407873:scorpine