A satellite DNA containing CENP-B box-like motifs is present in the antarctic scallop Adamussium colbecki.
The DNA of the Antarctic scallop Adamussium colbecki was found to contain a highly repeated sequence identifiable upon restriction with endonuclease BglII. The monomeric unit - denominated pACS (about 170bp long) - was cloned. Southern blot hybridization yielded a ladder-like banding pattern, indicating that the repeated elements are tandemly arranged in the genome and therefore represent a sequence of satellite DNA. Sequence analysis of five different clones revealed the presence of various subfamilies, some of which showed a high degree of divergence. In each clone, regions homologous to the mammalian CENP-B box were observed. A region homologous to the CDEIII centromeric sequence of yeast was also found in one of the clones. These observations suggest a relationship of the pACS family to the centromeric area in A. colbecki.
Canapa
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A
Istituto di Biologia e Genetica, Facoltà di Scienze, Università degli Studi di Ancona, via Brecce Bianche, I-60131, Ancona, Italy. a.canapa@popcsi.unian.it
Barucca
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M
Cerioni
P N
PN
Olmo
E
E
D000818:Animals; D000864:Antarctic Regions; D001324:Autoantigens; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D001324:Autoantigens; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D004247:DNA