title: Routine HLA-B genotyping with PCR-sequence-specific oligonucleotides (PCR-SSO) detects eight new alleles: B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508.
abstract: This paper describes eight new alleles (B*0807, B*0809, B*1551, B*3529, B*3532, B*4025, B*5304 and B*5508) that have been found by routine HLA-B genotyping with sequence-specific oligonucleotides (SSOs). All of the new alleles have variations which cause changes in residues that occur within antigen binding pockets and T-cell recognition sites of the antigen. The new polymorphisms within these new alleles may affect the nature and specificity of peptide binding and cause differential T-cell activation, which may have an affect in transplantation.
authors.affiliation: Molecular Genetics Laboratory, Tissue Typing, Australian Red Cross Blood Service, Sydney, New South Wales, Australia. ckennedy@arcbs.redcross.org.au
authors.forename: C T
authors.initials: CT
authors.lastname: Kennedy
authors.forename: R
authors.initials: R
authors.lastname: Dodd
authors.forename: T
authors.initials: T
authors.lastname: Le
authors.forename: R
authors.initials: R
authors.lastname: Wallace
authors.forename: G
authors.initials: G
authors.lastname: Ng
authors.forename: W D
authors.initials: WD
authors.lastname: Greville
authors.forename: A
authors.initials: A
authors.lastname: Kennedy
authors.forename: A
authors.initials: A
authors.lastname: Taverniti
authors.forename: J H
authors.initials: JH
authors.lastname: Moses
authors.forename: N
authors.initials: N
authors.lastname: Clow
authors.forename: N
authors.initials: N
authors.lastname: Watson
authors.forename: H
authors.initials: H
authors.lastname: Dunckley
mesh_terms: D000483:Alleles; D001483:Base Sequence; D018076:DNA, Complementary; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D059910:HLA-B15 Antigen; D015797:HLA-B35 Antigen / Q000235:genetics; D059947:HLA-B40 Antigen; D015795:HLA-B8 Antigen / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D018076:DNA, Complementary; D006680:HLA Antigens; D015235:HLA-B Antigens; D059910:HLA-B15 Antigen; D015797:HLA-B35 Antigen; D059947:HLA-B40 Antigen; C078438:HLA-B53 antigen; C082944:HLA-B55 antigen; D015795:HLA-B8 Antigen; D015345:Oligonucleotide Probes