title: Nerve growth factor and Trk high affinity receptor (TrkA) gene expression in inflammatory bowel disease.
abstract: BACKGROUND
  Nerve growth factor (NGF), a target derived factor for survival and maintenance of peripheral and central neurones, has been implicated in several chronic inflammatory processes.
  
  
  OBJECTIVE
  To analyse the concomitant presence of NGF and its high affinity receptor TrkA in patients undergoing surgery for Crohn's disease (CD) and ulcerative colitis (UC).
  
  
  METHODS
  CD tissues were obtained from 33 patients and UC tissue samples from 12 patients undergoing surgery. Normal intestinal tissue samples were obtained from 30 individuals through an organ donor programme.
  
  
  METHODS
  Expression of NGF and TrkA was studied by northern blot analysis. Using in situ hybridisation and immunohistochemistry, the respective mRNA moieties and proteins were localised. Western blot analysis was used to confirm the specificity of NGF and TrkA antibodies.
  
  
  RESULTS
  In CD, NGF mRNA was increased in 60% (2.4-fold; p<0.01) and TrkA mRNA in 54% (1.3-fold; p<0.05) of samples. In UC, NGF mRNA expression was enhanced in 58% (2.4-fold; p<0.01) and TrkA mRNA expression in 50% (1.5-fold; p<0.05) of samples. In situ hybridisation showed that NGF and TrkA mRNA were often concomitantly present in polymorphonuclear-like cells of the lamina propria, in mast cells, and in a few ganglia of Auerbach's plexus and Meissner's plexus. Immunohistochemistry revealed that lamina propria cells and inflammatory cells (mainly mast cells) were NGF and TrkA immunopositive. NGF was also present in Meissner's plexus (especially in CD) and TrkA in enteric glia surrounding intestinal ganglia.
  
  
  CONCLUSIONS
  The concomitant enhanced expression of NGF and its receptor suggests activation of this pathway in chronic inflammation in CD and UC. The presence of NGF and TrkA in both neural and non-neural structures in CD and UC supports the hypothesis that neuroimmune interactions occur and are activated in both disorders.
authors.affiliation: Department of Visceral and Transplantation Surgery, University of Bern, Inselspital, Switzerland.
authors.forename: F F
authors.initials: FF
authors.lastname: di Mola
authors.forename: H
authors.initials: H
authors.lastname: Friess
authors.forename: Z W
authors.initials: ZW
authors.lastname: Zhu
authors.forename: A
authors.initials: A
authors.lastname: Koliopanos
authors.forename: T
authors.initials: T
authors.lastname: Bley
authors.forename: P
authors.initials: P
authors.lastname: Di Sebastiano
authors.forename: P
authors.initials: P
authors.lastname: Innocenti
authors.forename: A
authors.initials: A
authors.lastname: Zimmermann
authors.forename: M W
authors.initials: MW
authors.lastname: Büchler
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000918:Antibody Specificity; D015152:Blotting, Northern; D015153:Blotting, Western; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism*; D003424:Crohn Disease / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D020917:Receptor, trkA / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D020932:Nerve Growth Factor; D020917:Receptor, trkA