Two systems for phosphate uptake in Yarrowia lipolytica cells grown at acidic conditions.
Inorganic phosphate (Pi) is accumulated by Yarrowia lipolytica cells grown at acidic pH conditions by two kinetically discrete H+/Pi-cotransport systems with apparent K(m) values for Pi of 12-18 microM and 2-3 mM Pi at pH 5.5, respectively. One of these is derepressible and operates at low external Pi concentrations; the other is most likely constitutively expressed and comes into play at high Pi concentrations. The derepression of the high-affinity Pi transport system is under the control of available extracellular Pi as well as the amount of intracellular polyphosphates stores. Characteristics of the Pi transport behavior in Yarrowia lipolytica are discussed.
Zvyagilskaya
R
R
A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia. inbio@glas.apc.org
Allard
P
P
Persson
B L
BL
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism*; D015003:Yeasts / Q000254:growth & development / Q000378:metabolism*
D010710:Phosphates