Vagus nerve is involved in lack of blood reflow into sinusoids after rat hepatic ischemia.
Although recovery of microcirculation is an important determinant for ischemia-reperfusion injury, little information is available about hepatic blood flow after ischemia. To examine regulatory mechanisms of postischemic hepatic microcirculation, we studied the sinusoidal blood flow after portal triad clamping of rat livers for 5, 15, or 30 min. Hepatic tissue blood flow and erythrocyte blood flow in sinusoids were measured using a laser-Doppler flowmeter and an intravital microspectroscope, respectively. There was a time of no blood flow (lag time) in sinusoids after declamping, dependent on the ischemic time. Cholinergic blockade agents eliminated the lag time, whereas nerve stimulation at the hiatus esophagus or on the hepatoduodenal ligament during reperfusion prolonged it. Chemical denervation with 10% phenol or surgical denervation on the hepatoduodenal ligament eliminated the lag time. The prolongation of lag time by nerve stimulation was completely abrogated by truncal vagotomy. These results suggest that the cholinergic vagus nerve is involved in causing the lag time of sinusoidal blood flow in hepatic ischemia-reperfusion.
Nishida
T
T
Department of Surgery, Osaka University Medical School, Suita, Osaka 565-0871, Japan. toshin@surg1.med.osaka-u.ac.jp
Ueshima
S
S
Kazuo
H
H
Ito
T
T
Seiyama
A
A
Matsuda
H
H
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D018680:Cholinergic Antagonists / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006499:Hepatic Artery / Q000503:physiopathology / Q000601:surgery; D007511:Ischemia; D017078:Laser-Doppler Flowmetry; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000503:physiopathology; D008102:Liver Circulation / Q000187:drug effects; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D018727:Muscarinic Antagonists / Q000494:pharmacology; D010275:Parasympathetic Nervous System / Q000187:drug effects / Q000502:physiology; D011169:Portal Vein / Q000503:physiopathology / Q000601:surgery; D051381:Rats; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000503:physiopathology*; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology; D014630:Vagus Nerve / Q000503:physiopathology*; D014665:Vasodilator Agents / Q000494:pharmacology
D000925:Anticoagulants; D018680:Cholinergic Antagonists; D004791:Enzyme Inhibitors; D018727:Muscarinic Antagonists; D014665:Vasodilator Agents