Inhibitory effects of of L-2'-deoxyguanosine 5'-triphosphate (L-dGTP) and L-2'-deoxythymidine 5'-triphosphate (L-dTTP) on human telomerase.
Telomerase, which synthesizes telomeric DNA in eukaryotic cells, is classified as a reverse transcriptase. To clarify the recognition of 2'-deoxyribonucleoside 5'-triphosphate (dNTP) chirality by telomerase, we studied the inhibitory effects of L-dGTP on HeLa cell telomerase activity using a quantitative 'stretch PCR' assay. L-dGTP had a weakly inhibitory effect (IC50 = 200 microM) in the presence of 10 microM dGTP. This effect was less obvious when the concentration of dGTP was higher. L-dTTP had a similar inhibitory effect. These findings suggest that telomerase may bind to L-dGTP and L-dTTP, and that the ability of telomerase to bind to dGTP or dTTP is changed.
Yamaguchi
T
T
Department of Biological Sciences, Teikyo University of Science and Technology, Uenohara, Yamanashi, Japan.
Yamada
R
R
Shudo
K
K
Saito
M
M
Ishikawa
F
F
Saneyoshi
M
M
D001483:Base Sequence; D017931:DNA Primers; D003848:Deoxyguanine Nucleotides / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000379:methods; D013237:Stereoisomerism; D019098:Telomerase / Q000037:antagonists & inhibitors*; D013942:Thymine Nucleotides / Q000737:chemistry / Q000494:pharmacology*
D017931:DNA Primers; D003848:Deoxyguanine Nucleotides; D013942:Thymine Nucleotides; C029603:deoxyguanosine triphosphate; D019098:Telomerase; C024157:thymidine 5'-triphosphate