Extended in vitro selection for synthesis of novel molecular recognition oligonucleotide derivatives.
In vitro selection of RNA aptamer containing biotin-carrying nucleotide was carried out used for development of a new type of molecular sensor. Cytidine 5'-triphosphate (CTP) carrying biotinyl group at the N6-position was used in this technique. A pool of biotin-containing RNAs, which binds specifically to adenosine 5'-triphosphate (ATP), was obtained and used for competitive binding assay of ATP. The selected nonnatural RNA possesses many biotinyl groups to render it a high sensitivity toward ATP.
Kawazoe
N
N
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Japan.
Ito
Y
Y
D000255:Adenosine Triphosphate / Q000737:chemistry*; D001667:Binding, Competitive; D019921:Biotinylation; D003570:Cytidine Triphosphate / Q000031:analogs & derivatives* / Q000737:chemistry*; D007700:Kinetics; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry*
D012313:RNA; D003570:Cytidine Triphosphate; D000255:Adenosine Triphosphate