Heat-induced relocalization of protein kinase CK2. Implication of CK2 in the context of cellular stress.
Among various other roles described so far, protein kinase CK2 has been involved in cell cycle, proliferation, and development. Here, we show that in response to specific stresses (heat shock or UV irradiation), a pool of the cellular CK2 content relocalizes in a particular nuclear fraction, increasing the activity of the kinase there. Electron microscopic analysis shows that upon heat shock, CK2alpha and CK2beta subunits are both detected in similar speckle structures occurring in the interchromatin space but are differentially targeted inside the nucleolus. This CK2 relocalization process takes place in a time- and dose-dependent manner and is reversible upon recovery at 37 degrees C. Altogether, this work suggests CK2 be involved in the response to physiological stress in higher eukaryotic cells.
Gerber
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Laboratoire INSERM U244, CENG, 17 Avenue des Martyrs, 38000 Grenoble, France.
Souquere-Besse
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Puvion
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Dubois
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MF
Bensaude
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O
Cochet
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C
D016475:3T3 Cells; D000818:Animals; D001692:Biological Transport; D047390:Casein Kinase II; D002451:Cell Compartmentation; D002466:Cell Nucleolus / Q000378:metabolism*; D006367:HeLa Cells; D006706:Homeostasis; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D018384:Oxidative Stress; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013347:Subcellular Fractions; D014466:Ultraviolet Rays
D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases