Cloning and expression of the M5 RNA segment encoding outer capsid VP5 of epizootic hemorrhagic disease virus Japan serotype 2, Ibaraki virus.
The complete nucleotide sequence of a cDNA clone representing the M5 RNA segment of epizootic hemorrhagic disease virus Japan serotype 2 (EHDV-2), Ibaraki virus, was determined. The M5 segment is 1641 base pairs long with the single open reading frame which predicts a polypeptide of 527 amino acids. The comparison of the amino acid sequence of the VP5 with those of EHDV-1, bluetongue virus serotype 10, and African horse sickness virus serotype 4 revealed that the protein shared 67%, 57% and 42% homologies, respectively. In addition, the VP5 protein was expressed in insect cells by recombinant baculovirus, which could be recognized by the mouse anti-EHDV-2 sera at a position of the expected 59 kDa on immunoblot analysis.
Nara Pereira
E M
EM
Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Japan.
Nishida
T
T
Tokunaga
R
R
Iwata
H
H
Inoue
T
T
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera
D036022:Capsid Proteins; D012367:RNA, Viral; C411966:VP5, Ibaraki virus