Biotransformations monitored in situ by proton nuclear magnetic resonance spectroscopy.
One-dimensional Fourier-transform proton nuclear magnetic resonance (1H-NMR) spectroscopy can be used to study biotransformations in situ, in vivo and in aqua (1H2O). Although an insensitive method, it rapidly provides solution-structural information of mixtures of diverse compounds that are used and formed during enzymic reactions and culture fermentations; the samples do not require any physical or chemical processing for analysis. The absolute stereochemistry of some reactions can also be determined, and assessments of metabolic fluxes made. This technique, with appropriate modifications, is of obvious value for on-line assessments of industrial fermentation processes.
Brecker
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Institute of Organic Chemistry, Technical University Graz, Stremayrgasse 16, A-8010 Graz, Austria.
Ribbons
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D000332:Aerobiosis; D000446:Aldehyde-Lyases / Q000032:analysis / Q000378:metabolism; D001711:Biotransformation; D002478:Cells, Cultured; D004798:Enzymes / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D005583:Fourier Analysis; D008049:Lipase / Q000032:analysis / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D012997:Solvents
D004798:Enzymes; D012997:Solvents; D008049:Lipase; D000446:Aldehyde-Lyases; C079359:hydroxymandelonitrile lyase