Immobilization of DNA by UV irradiation and its utilization as functional materials.
The water-insoluble DNA film was successfully prepared by UV irradiation. The DNA film was stable in water. It could effectively accumulated the DNA-binding intercalating materials, such as ethidium bromide, dibenzo-p-dioxin and benzo[a]pyrene, in their aqueous solutions. On the other hand, DNA was immobilized onto nonwoven cellulose fabrics, also by the UV irradiation. The DNA immobilized cloth was found to bind silver ions. The DNA-cloth containing silver ion showed antibacterial activity. The water-insoluble DNA prepared by UV irradiation has a potential ability to serve as biomaterials for medical, engineering and environmental objects.
Yamada
M
M
Division of Bioscience, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Kato
K
K
Shindo
K
K
Nomizu
M
M
Sakairi
N
N
Yamamoto
H
H
Nishi
N
N
D000818:Animals; D001564:Benzo(a)pyrene / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D004147:Dioxins / Q000737:chemistry; D004996:Ethidium / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D008297:Male; D012474:Salmon; D012996:Solutions; D013053:Spectrophotometry; D013094:Spermatozoa; D014466:Ultraviolet Rays
D004147:Dioxins; D007364:Intercalating Agents; D012996:Solutions; D001564:Benzo(a)pyrene; D004247:DNA; D004996:Ethidium; C020887:dibenzo(1,4)dioxin