title: [Modeling of the effect of modulated electromagnetic radiation on animal cells].
abstract: Frequency-dependent modifications of intracellular free calcium concentration ([Ca2+]i) in neutrophils exposures to modulated extremely high frequency electromagnetic radiation were analyzed using a special mathematical model for [Ca2+]i oscillations. The model took into account the activation of Ca2+ influx into the cell by cytosolic Ca2+ and Ca(2+)-induced Ca2+ release from intracellular stores. The calcium channels of plasma membrane were chosen as a target for the influence of harmonic signal and additive noise in the model. The model simulation showed that in response to modulating signal, the rise in [Ca2+]i, has frequency dependence and phase dependence in relation to the moment of chemical stimulation. The phase-frequency dependence of the effect was observed at a certain sequence of delivery of chemical stimulus and modulating signal to the cell. At intensities of modulating signals exceeding the threshold, a rise in [Ca2+]i, reaching a level of more than 50% of the initial level, was observed at a frequency of about 1 Hz and in the phase range of 0.3-2.5 radians. The effect was found only at high intensities of chemical stimulus. The additive noise introduced into the system modified qualitatively and quantitatively the phase-frequency characteristics of the cell response to the modulating signal. An increase in noise intensity resulted in a displacement of the average frequency of the band of rise in [Ca2+]i, and then the emergence of a set of bands with a greater Q-factors. The analysis of dynamics of the nonlinear system in terms of the stability theory showed that, as the intensity of chemical stimulus increases, the system transits by means of a series of bifurcations from regular driving to chaotic, and then to oscillations, induced by a modulating harmonic signal. The boundary of the transition of oscillations from chaotic to induced ones corresponds to a specific "threshold" of the intensity of chemical stimulus for the significant rise in [Ca2+]i in response to the modulating signal. The results of the model analysis are in good correspondence with the experimental data obtained earlier, namely, with the effects of modulated extremely high-frequency electromagnetic radiation on neutrophils, which were observed only in the presence of Ca2+ in extracellular medium and at high concentrations of calcium ionophore A23187. Thus, as the characteristic frequency of the quasi-periodic process of calcium signalling in the cell coincides with the frequency of external field, a narrow-band rise in [Ca2+]i is observed, which can result in a modification of the functional activity of the cell.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Gapeev
authors.forename: N K
authors.initials: NK
authors.lastname: Chemeris
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D008872:Microwaves; D008954:Models, Biological; D009504:Neutrophils / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure
chemical_list: D015220:Calcium Channels; D002118:Calcium