Temporal profile of release of interleukin-1beta in neurotrauma.
Timing and extent of trauma-induced release of interleukin-1beta (IL-1beta) in extracellular fluid of the CNS were analyzed. In brain tissue perfusates obtained by in vivo microdialysis a marked release of IL-1beta was unexpectedly detected within less than 60 min. At such an early stage of neurotrauma, mRNA expression of IL-1beta was detected whereas immunoreactivity for the IL-1beta protein was negative. Concentrations of extracellularly secreted IL-1beta protein gradually increased, peaked at day 2 and decreased thereafter. Drugs acting on mononuclear phagocytes significantly modulated IL-1beta secretion. This so far unrecognized acuity of IL-1beta release demonstrated here, may represent a precondition for the orchestrating role of this mediator in the cascade of inflammatory host response.
Fassbender
K
K
Department of Neurology, University Clinic Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, D-68135, Mannheim, Germany. fass@neuro.ma.uni-heidelberg.de
Schneider
S
S
Bertsch
T
T
Schlueter
D
D
Fatar
M
M
Ragoschke
A
A
Kühl
S
S
Kischka
U
U
Hennerici
M
M
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001930:Brain Injuries / Q000378:metabolism*; D002738:Chloroquine / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D017551:Microdialysis; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D015843:Serpins / Q000494:pharmacology; D013997:Time Factors; D014764:Viral Proteins
D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D015843:Serpins; D014764:Viral Proteins; D007371:Interferon-gamma; D002738:Chloroquine; C074641:interleukin-1beta-converting enzyme inhibitor; D003078:Colchicine