title: Ultracytochemical localization of 5'-nucleotidase activity in human ejaculated spermatozoa.
abstract: 5'-nucleotidase, an adenosine producing enzyme with a glycosylphosphatidylinositol-anchored structure, was localized in human ejaculated spermatozoa. The poly-L-lysine-coated dish method was used to prepare the specimens, and the cerium method was employed for electron-microscopic enzyme localization. Precipitates indicating enzyme activity were detected on the outer side of the external plasma membrane of the acrosomal region. This enzyme may play a role in sperm motility and male infertility.
authors.affiliation: Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan. obtakegy@jichi.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Takayama
authors.forename: S
authors.initials: S
authors.lastname: Matsubara
authors.forename: H
authors.initials: H
authors.lastname: Shibahara
authors.forename: H
authors.initials: H
authors.lastname: Minakami
authors.forename: T
authors.initials: T
authors.lastname: Takizawa
authors.forename: I
authors.initials: I
authors.lastname: Sato
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis*; D004542:Ejaculation; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D015720:5'-Nucleotidase