title: Calcineurin controls the transcription of Na+/Ca2+ exchanger isoforms in developing cerebellar neurons.
abstract: The Na(+)/Ca(2+) exchanger (NCX) and the plasma membrane Ca(2+)-ATPase export Ca(2+) from the cytosol to the extracellular space. Three NCX genes (NCX1, NCX2, and NCX3), encoding proteins with very similar properties, are expressed at different levels in tissues. Essentially, no information is available on the mechanisms that regulate their expression. Specific antibodies have been prepared and used to explore the expression of NCX1 and NCX2 in rat cerebellum. The expression of NCX2 became strongly up-regulated during development, whereas comparatively minor effects were seen for NCX1. This was also observed in cultured granule cells induced to mature in physiological concentrations of potassium. By contrast, higher K(+) concentrations, which induce partial depolarization of the plasma membrane and promote the influx of Ca(2+), caused the complete disappearance of NCX2. Reverse transcription-polymerase chain reaction analysis showed that the process occurred at the transcriptional level and depended on the activation of the Ca(2+) calmodulin-dependent protein phosphatase, calcineurin. The NCX1 and NCX3 genes were also affected by the depolarizing treatment: the transcription of the latter became up-regulated, and the pattern of expression of the splice variants of the former changed. The effects on the NCX1 and NCX3 genes were calcineurin-independent.
authors.affiliation: Institute of Biochemistry, Swiss Federal Institute of Technology, 8092 Zürich, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Guerini
authors.forename: E
authors.initials: E
authors.lastname: Carafoli
mesh_terms: D000818:Animals; D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002531:Cerebellum / Q000378:metabolism; D004789:Enzyme Activation; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D026901:Membrane Transport Proteins; D009474:Neurons / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011233:Precipitin Tests; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D019831:Sodium-Calcium Exchanger / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D026901:Membrane Transport Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; C101832:SLC38A3 protein, human; C110927:Slc8a2 protein, rat; C491102:Slc8a3 protein, rat; D019831:Sodium-Calcium Exchanger; C110374:sodium-calcium exchanger 1; D019703:Calcineurin; D011188:Potassium; D002118:Calcium