The sorcin-annexin VII calcium-dependent interaction requires the sorcin N-terminal domain.
Surface plasmon resonance experiments show that at neutral pH the stability of the complex between sorcin and annexin VII (synexin) increases dramatically between 3 and 6 microM calcium; at the latter cation concentration the K(D) value is 0.63 microM. In turn, the lack of complex formation between the sorcin Ca(2+) binding domain (33-198) and synexin maps the annexin binding site to the N-terminal region of the sorcin polypeptide chain. Annexin VII likewise employs the N-terminal domain, more specifically the first 31 amino acids, to interact with sorcin [Brownawell, A.M. and Creutz, C.E. (1997) J. Biol. Chem. 272, 22182-22190]. The interaction may involve similar structural motifs in the two proteins, namely GGYY and GYGG in sorcin and GYPP in synexin.
Verzili
D
D
CNR Center of Molecular Biology, Department of Biochemical Sciences 'A. Rossi Fanelli', University of Rome 'La Sapienza', P.le A. Moro 5, 00185, Rome, Italy.
Zamparelli
C
C
Mattei
B
B
Noegel
A A
AA
Chiancone
E
E
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D017310:Annexin A7 / Q000378:metabolism*; D001665:Binding Sites; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000276:immunology; D007700:Kinetics; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D020349:Surface Plasmon Resonance; D013816:Thermodynamics
D017310:Annexin A7; D002135:Calcium-Binding Proteins; D007106:Immune Sera; D010446:Peptide Fragments; D011994:Recombinant Proteins; C503114:Sri protein, mouse; D004533:Egtazic Acid; D002118:Calcium