Inhibitor of tumor necrosis factor-alpha production in lipopolysaccharide-stimulated RAW264.7 cells from Amorpha fruticosa.
Certain flavonoids were reported to show an immunoregulatory activity against lymphocyte proliferation and cytokine production. In the course of a search for tumor necrosis factor (TNF)-alpha inhibitory compounds from natural plants, we also isolated a prenylfavanone type of flavonoid, amoradicin, from the extract of Amorpha fruticosa by activity-guided fractionation. This compound significantly inhibited TNF-alpha production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells with an IC(50) value of 28.5 microM. The activity was comparable or higher than those of standard flavonoid compounds, genistein and silybin with IC(50) of 24.9 and 140.3 microM, respectively.
Cho
J Y
JY
Department of Inflammation, Allergy and Autoimmune Diseases, R & D Center, Daewoong Pharmaceutical Co. Ltd., Sungnam, South Korea. jae.cho@ucl.ac.uk
Kim
P S
PS
Park
J
J
Yoo
E S
ES
Baik
K U
KU
Kim
Y K
YK
Park
M H
MH
D000818:Animals; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology; D007723:Korea; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D012838:Silymarin / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D005419:Flavonoids; D008070:Lipopolysaccharides; D012838:Silymarin; D014409:Tumor Necrosis Factor-alpha; D019833:Genistein