Determination of 18alpha-glycyrrhizin and 18beta-glycyrrhizin in dog plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic method has been developed for the separation and determination of 18alpha-glycyrrhizin (alpha-GZ) and 18beta-glycyrrhizin (beta-GZ) in dog plasma. The two compounds were separated on a reversed-phase column and detected by UV absorption at 254 nm. The mobile phase was a mixture of water-methanol-60% perchloric acid (45:55:0.5, v/v) and was adjusted to pH 8.0 with 25% ammonia solution. Indomethacin was added to the plasma as an internal standard. Methanol was selected for the extraction of both the compounds and internal standard. Alpha-GZ and beta-GZ could be precisely determined in concentration of 1 mg/ml in a 0.1 ml sample.
Koga
K
K
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan. k-koga@hokuriku-u.ac.jp
Ohmachi
K
K
Kawashima
S
S
Takada
K
K
Murakami
M
M
D000641:Ammonia; D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D019695:Glycyrrhizic Acid / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D010472:Perchlorates; D012680:Sensitivity and Specificity; D014867:Water
D000890:Anti-Infective Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010472:Perchlorates; D014867:Water; D019695:Glycyrrhizic Acid; D000641:Ammonia; D000432:Methanol