Changes in lipolysis and hormone-sensitive lipase expression caused by procyanidins in 3T3-L1 adipocytes.
OBJECTIVE
To find out whether lipid stores are influenced by phenolic compounds in wine.


METHODS
Differentiated 3T3-L1 cells were treated with catechin, epicatechin or procyanidin extracts with different degrees of polymerization at 150 microM for different periods of time (0.5-24 h).


METHODS
Cell line 3T3-L1.


METHODS
Cellular viability, glycerol-3-phosphate dehydrogenase activity, glycerol release in the medium, HSL mRNA levels, triacylglycerols and protein.


RESULTS
Catechin, epicatechin and procyanidin extracts were not toxic for the 3T3-L1 cells in the conditions assayed. Glycerol-3-phosphate dehydrogenase activity was markedly decreased by 150 microM procyanidin extracts. The release of glycerol into the medium was increased in 150 microM procyanidin extract-treated cells and reached a plateau after 15 h exposure. Procyanidins caused a time-dependent reduction in the HSL mRNA levels.


CONCLUSIONS
These results suggest that procyanidins from grape and wine affect lipid metabolism whilst their monomers (catechin and epicatechin) do not. This effect is more pronounced when the degree of polymerization is higher. Procyanidin extracts cause a time-dependent reduction in the HSL mRNA levels, inhibit triacylglycerol synthesis and also favour triacylglycerol hydrolysis until the HSL mRNA had reached very low levels.
Ardévol
A
A
Departament de Bioquimica i Biotecnologia, CeRTA, Universitat Rovira i Virgili, Tarragona, Spain. aag@astor.urv.es
Bladé
C
C
Salvadó
M J
MJ
Arola
L
L
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D044946:Biflavonoids; D002392:Catechin / Q000494:pharmacology*; D002470:Cell Survival; D015870:Gene Expression / Q000187:drug effects*; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007700:Kinetics; D008066:Lipolysis; D051379:Mice; D010636:Phenols / Q000494:pharmacology; D044945:Proanthocyanidins; D012333:RNA, Messenger / Q000032:analysis; D002787:Sterol Esterase / Q000235:genetics* / Q000378:metabolism; D014280:Triglycerides / Q000032:analysis; D014920:Wine / Q000032:analysis*
D044946:Biflavonoids; D010636:Phenols; D044945:Proanthocyanidins; D012333:RNA, Messenger; D014280:Triglycerides; C017674:procyanidin; D002392:Catechin; D005993:Glycerolphosphate Dehydrogenase; D002787:Sterol Esterase; D005990:Glycerol