title: Imaging membrane protein helical wheels.
abstract: Resonance patterns have been observed in 2D solid-state NMR spectra of the transmembrane segment of M2 protein from Influenza A virus in oriented samples reflecting the helical wheel of this alpha-helix. The center of this pattern uniquely defines the helical tilt with respect to the bilayer normal without a need for resonance assignments. The distribution of resonances from amino acid specific labels around the "PISA wheel" defines the rotational orientation of the helix and yields preliminary site-specific assignments. With assignments high-resolution structural detail, such as differences in tilt and rotational orientation along the helical axis leading to an assessment of helical coiling, can be obtained.
authors.affiliation: National High Magnetic Field Laboratory, Florida State University, Tallahassee, Florida 32310, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Denny
authors.forename: C
authors.initials: C
authors.lastname: Tian
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Mo
authors.forename: F
authors.initials: F
authors.lastname: Kovacs
authors.forename: Z
authors.initials: Z
authors.lastname: Song
authors.forename: K
authors.initials: K
authors.lastname: Nishimura
authors.forename: Z
authors.initials: Z
authors.lastname: Gan
authors.forename: R
authors.initials: R
authors.lastname: Fu
authors.forename: J R
authors.initials: JR
authors.lastname: Quine
authors.forename: T A
authors.initials: TA
authors.lastname: Cross
mesh_terms: D009980:Influenza A virus; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D017433:Protein Structure, Secondary
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D009587:Nitrogen Isotopes