Differential expression of peroxisome proliferator-activated receptors (PPARs) in the developing human fetal digestive tract.
We investigated the spatiotemporal distributions of the different peroxisome proliferator-activated receptor (PPAR) isotypes (alpha, beta, and gamma) during development (Week 7 to Week 22 of gestation) of the human fetal digestive tract by immunohistochemistry using specific polyclonal antibodies. The PPAR subtypes, including PPARgamma, are expressed as early as 7 weeks of development in cell types of endodermal and mesodermal origin. The presence of PPARgamma was also found by Western blotting and nuclease-S1 protection assay, confirming that this subtype is not adipocyte-specific. PPARalpha, PPARbeta, and PPARgamma exhibit different patterns of expression during morphogenesis of the digestive tract. Whatever the stage and the gut region (except the stomach) examined, PPARgamma is expressed at a high level, suggesting some fundamental role for this receptor in development and/or physiology of the human digestive tract.
Huin
C
C
Laboratoire de Biologie Cellulaire du Développement, EA 2402 "Proliférateurs de Peroxysomes," Faculté des Sciences, Vandoeuvre-les-Nancy, France.
Corriveau
L
L
Bianchi
A
A
Keller
J M
JM
Collet
P
P
Krémarik-Bouillaud
P
P
Domenjoud
L
L
Bécuwe
P
P
Schohn
H
H
Ménard
D
D
Dauça
M
M
D000918:Antibody Specificity; D015153:Blotting, Western; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D003106:Colon / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004064:Digestive System / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D004947:Esophagus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis*; D013270:Stomach / Q000166:cytology / Q000196:embryology; D014157:Transcription Factors / Q000096:biosynthesis*
D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors