[Independent denaturation of albumin and globulins in human serum].
The independent melting of albumin, gamma-globulin, transferrin, and protease inhibitors in the composition of donor blood serum was studied by differential scanning microcalorimetry. It was found that the number of domains in gamma-globulin in donor blood serum and in diluted solutions is the same, whereas the number of domains of albumin in solution and in the composition of blood serum is three and two, respectively. In blood serum, the N-terminal domain melts by the "all-or-none" mechanism. Therefore, the decomposition of peaks of the denaturation curve was made under the assumption that the denaturation of blood serum proteins occurs by the "all-or-none" principle. It is assumed that comparing the calculated melting parameters (Td, delta Td, delta Hd, delta Cd) of domains of blood serum proteins of donor with the corresponding parameters of patients with oncological and nononcological diseases can be used as a basis for a more precise diagnostics of these diseases.
Khachidze
D G
DG
Institute of Physics, Academy of Sciences of Georgia, Tbilisi, Georgia.
Monaselidze
D R
DR
D002152:Calorimetry, Differential Scanning; D005916:Globulins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011480:Protease Inhibitors / Q000737:chemistry / Q000378:metabolism; D011489:Protein Denaturation; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism
D005916:Globulins; D011480:Protease Inhibitors; D012709:Serum Albumin; D014168:Transferrin