Preparation and characterization of collagen-elastin matrices from blood vessels intended as small diameter vascular grafts.
This study describes the selective removal of cell and cell residues from small and large diameter blood vessels for the preparation of tubular collagen:elastin matrices intended for small diameter vascular prosthesis. The results showed that total or partially devitalized collagen:elastin matrices may be conveniently prepared without denaturation of the collagen:elastin matrix with a high degree of preservation of the proteins. The efficiency of cell removal and the extent collagen and elastic fiber preservation were dependent on the segment and the type of blood vessel under study, with arteries characterized by a higher susceptibility of cell removal and better preservation of the collagen-elastin matrix.
Goissis
G
G
Department of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo;Faculty of Medicine São José do Rio Preto, São Paulo, Brazil. ggoissis@iqsc.cs.usp.br
Suzigan
S
S
Parreira
D R
DR
Maniglia
J V
JV
Braile
D M
DM
Raymundo
S
S
D000468:Alkalies; D000818:Animals; D001012:Aorta, Abdominal; D001672:Biocompatible Materials; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000166:cytology; D002152:Calorimetry, Differential Scanning; D003094:Collagen; D004396:Coloring Agents; D003432:Cross-Linking Reagents; D004121:Dimethyl Sulfoxide; D004285:Dogs; D004549:Elastin; D004594:Electrophysiology; D005263:Femoral Artery; D005268:Femoral Vein; D007083:Iliac Artery; D007084:Iliac Vein; D008855:Microscopy, Electron, Scanning; D011474:Prosthesis Design; D012997:Solvents; D013895:Thoracic Arteries; D014021:Tissue Preservation / Q000379:methods*; D014682:Vena Cava, Inferior; D014683:Vena Cava, Superior
D000468:Alkalies; D001672:Biocompatible Materials; D004396:Coloring Agents; D003432:Cross-Linking Reagents; D012997:Solvents; D003094:Collagen; D004549:Elastin; D004121:Dimethyl Sulfoxide