Murine polyomavirus infection of 3T6 mouse cells shows evidence of predominant necrosis as well as limited apoptosis.
The current study was developed to determine if polyomavirus infected 3T6 mouse cells evoked an apoptotic or a necrotic mechanism during infection. Infected cells were analyzed by flow cytometry, transmission electron microscopy (TEM), DNA electrophoresis and by measuring caspase-3 enzymatic activity. Infected cells that were analyzed at 72 h post-infection showed the following: flow cytometry analysis revealed a 5% increase in apoptotic cells and a 46% increase in necrotic cells when compared to uninfected cells; electron microscopy showed 10% cells with characteristic apoptotic morphology and 40% with necrotic appearance; caspase-3 activity was found to increase two fold when compared to uninfected cells and DNA fragmentation (laddering) was clearly evident late in infection. It was concluded that infected cells predominantly showed necrosis, although some cells showed apoptosis in late infection. Recombinant capsid-like particles composed of the polyomavirus structural proteins were not able to induce cell death.
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Division of Biology-Ackert Hall, Section of Virology and Oncology, Kansas State University, Manhattan, KS 66506, USA.
Fattaey
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HK
Paulsen
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AQ
Consigli
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RA
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000032:analysis; D002460:Cell Line; D053938:DNA Fragmentation; D003609:Dactinomycin / Q000494:pharmacology; D005347:Fibroblasts / Q000821:virology*; D005434:Flow Cytometry; D051379:Mice; D008854:Microscopy, Electron; D009336:Necrosis; D011120:Polyomavirus / Q000472:pathogenicity*
D003609:Dactinomycin; D020169:Caspases