Glucoamylase production in batch, chemostat and fed-batch cultivations by an industrial strain of Aspergillus niger.
The Aspergillus niger strain BO-1 was grown in batch, continuous (chemostat) and fed-batch cultivations in order to study the production of the extracellular enzyme glucoamylase under different growth conditions. In the pH range 2.5-6.0, the specific glucoamylase productivity and the specific growth rate of the fungus were independent of pH when grown in batch cultivations. The specific glucoamylase productivity increased linearly with the specific growth rate in the range 0-0.1 h(-1) and was constant in the range 0.1-0.2 h(-1). Maltose and maltodextrin were non-inducing carbon sources compared to glucose, and the maximum specific growth rate was 0.19 +/- 0.02 h(-1) irrespective of whether glucose or maltose was the carbon source. In fed-batch cultivations, glucoamylase titres of up to 6.5 g l(-1) were obtained even though the strain contained only one copy of the glaA gene.
Pedersen
H
H
Department of Biotechnology, Technical University of Denmark, Lyngby.
Beyer
M
M
Nielsen
J
J
D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development*; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics
D003470:Culture Media; D002244:Carbon; D005087:Glucan 1,4-alpha-Glucosidase