title: The inhibitory effect of interferon gamma and tumor necrosis factor alpha on intracellular multiplication of Neospora caninum in primary bovine brain cells.
abstract: Primary culture of bovine brain cells was examined for its susceptibility to Neospora caninum infections, and this model was used to investigate the effects of bovine interferon gamma (IFN-gamma) and tumor necrosis factors alpha (TNF-alpha) on tachyzoite growth. Tachyzoites of N. caninum grew well in this culture, and tachyzoite growth in astroglia and microglia were confirmed by immunocytochemical staining. IFN-gamma inhibited the tachyzoite growth, and this inhibition was not reversed by the addition of nitric oxide antagonist. TNF-alpha, to a lesser extent, also inhibited the tachyzoite growth. Th-1 type cytokines may play an important role in host defense mechanisms in N. caninum infection.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yamane
authors.forename: H
authors.initials: H
authors.lastname: Kitani
authors.forename: T
authors.initials: T
authors.lastname: Kokuho
authors.forename: T
authors.initials: T
authors.lastname: Shibahara
authors.forename: M
authors.initials: M
authors.lastname: Haritani
authors.forename: T
authors.initials: T
authors.lastname: Hamaoka
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.forename: M
authors.initials: M
authors.lastname: Koiwai
authors.forename: K
authors.initials: K
authors.lastname: Shimura
authors.forename: Y
authors.initials: Y
authors.lastname: Yokomizo
mesh_terms: D000818:Animals; D001921:Brain / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002478:Cells, Cultured; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D007371:Interferon-gamma / Q000494:pharmacology*; D018688:Neospora / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma