Selective regulation of endogenous G protein-coupled receptors by arrestins in HEK293 cells.
Arrestins play an important role in regulating desensitization and trafficking of G protein-coupled receptors (GPCRs). However, limited insight into the specificity of arrestin-mediated regulation of GPCRs is currently available. Recently, we used an antisense strategy to reduce arrestin levels in HEK293 cells and characterize the role of arrestins on endogenous G(s)-coupled receptors (Mundell, S. J., Loudon, R. B., and Benovic, J. L. (1999) Biochemistry 38, 8723-8732). Here, we characterized GPCRs coupled to either G(q) (M(1) muscarinic acetylcholine receptor (M(1)AchR) and P2y(1) and P2y(2) purinergic receptors) or G(i) (somatostatin and AT1 angiotensin receptors) in wild type and arrestin antisense HEK293 cells. The agonist-specific desensitization of the M(1)Ach and somatostatin receptors was significantly attenuated in antisense-expressing cells, whereas desensitization of P2y(1) and P2y(2) purinergic and AT1 angiotensin receptors was unaffected by reduced arrestin levels. To further examine arrestin/GPCR specificity, we studied the effects of endogenous GPCR activation on the redistribution of arrestin-2 epitope tagged with the green fluorescent protein (arrestin-2-GFP). These studies revealed a receptor-specific movement of arrestin-2-GFP that mirrored the arrestin-receptor specificity observed in the antisense cells. Thus, agonist-induced activation of endogenous beta(2)-adrenergic, prostaglandin E(2), M(1)Ach, and somatostatin receptors induced arrestin-2-GFP redistribution to early endosomes, whereas P2y(1) and P2y(2) purinergic and AT1 angiotensin receptor activation did not. Thus, endogenous arrestins mediate the regulation of selective G(q)- and G(i)-coupled receptors in HEK293 cells.
Mundell
S J
SJ
Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Benovic
J L
JL
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D019390:Arrestins / Q000378:metabolism / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000378:metabolism; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016376:Oligonucleotides, Antisense / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011945:Receptors, Angiotensin / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism; D011983:Receptors, Purinergic / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014168:Transferrin / Q000378:metabolism; D014544:Uridine Triphosphate / Q000378:metabolism; D020696:rab5 GTP-Binding Proteins / Q000378:metabolism
D019390:Arrestins; D007295:Inositol Phosphates; D008164:Luminescent Proteins; D016376:Oligonucleotides, Antisense; D010750:Phosphoproteins; D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D011983:Receptors, Purinergic; D017481:Receptors, Somatostatin; D014168:Transferrin; C082333:arrestin3; D049452:Green Fluorescent Proteins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002217:Carbachol; D019204:GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins; D002118:Calcium; D014544:Uridine Triphosphate