Chick muscle-derived protein 62: a novel neurite outgrowth promoting protein.
A 3.2 kb chick cDNA clone that coded for a novel muscle-derived protein, MDP62, was isolated from a cDNA library of the denervated crus muscles using an antibody which inhibited the neurite (dendritic and axonal processes) outgrowth activity. MDP62 consisted of 539 aa with a calculated molecular mass of 62 k. The predicted protein sequence was hydrophilic and exhibited an extended coiled-coil domain and a leucine zipper motif. A recombinant protein promoted the neurite outgrowth from the cultured chick neurons of the telencephalon in a dose dependent manner. Northern blotting revealed that MDP77 was ubiquitously expressed. In the transfected COS-7 cells with the cDNA of the epitope-tagged MDP62, the expressed protein was detected in the culture medium, suggesting that the MDP62 might be secreted.
Uyeda
A
A
Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Japan.
Simo Wydisdtuti
M
M
Nishimune
H
H
Kiyosue
K
K
Kasai
M
M
Taguchi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D015152:Blotting, Northern; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D015723:Gene Library; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D013687:Telencephalon / Q000166:cytology / Q000187:drug effects / Q000196:embryology
D030161:Avian Proteins; D018076:DNA, Complementary; C410107:MDP62 protein, Gallus gallus; D009124:Muscle Proteins