title: Biochemical and genetic evidence for phospholipase C activity in Mycobacterium ulcerans.
abstract: This study reports the existence of phospholipase C and D enzymatic activities in Mycobacterium ulcerans cultures as determined by use of thin-layer chromatography to detect diglycerides in hydrolysates of radiolabeled phosphatidylcholine. M. ulcerans DNA sequences homologous to the genes encoding phospholipase C in Mycobacterium tuberculosis and Pseudomonas aeruginosa were identified by sequence analysis and DNA-DNA hybridization. Whether or not the phospholipase C and D enzymes of M. ulcerans plays a role in the pathogenesis of the disease needs further investigation.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp, Belgium. arley@itg.be
authors.forename: A
authors.initials: A
authors.lastname: Gomez
authors.forename: A
authors.initials: A
authors.lastname: Mve-Obiang
authors.forename: B
authors.initials: B
authors.lastname: Vray
authors.forename: J
authors.initials: J
authors.lastname: Remacle
authors.forename: K
authors.initials: K
authors.lastname: Chemlal
authors.forename: W M
authors.initials: WM
authors.lastname: Meyers
authors.forename: F
authors.initials: F
authors.lastname: Portaels
authors.forename: P A
authors.initials: PA
authors.lastname: Fonteyne
mesh_terms: D004269:DNA, Bacterial; D006801:Humans; D019911:Mycobacterium ulcerans / Q000201:enzymology* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010739:Phospholipase D / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D010738:Type C Phospholipases; D010739:Phospholipase D