A cDNA encoding the highly immunodominant antigen of Strongyloides stercoralis: gamma-subunit of isocitrate dehydrogenase (NAD+).
A full length cDNA encoding the highly immunodominant 41 kDa antigen of Strongyloides stercoralis (P5), recognized by 83% of human patients [Siddiqui et al. (1997) Parasitol Res 83:655-658], is obtained. A clone containing a 1371 bp insert was selected following screening of the S. stercoralis cDNA library with antibodies specific to antigen P5. The nucleotide sequence of this insert identified a cDNA coding for the gamma-subunit of isocitrate dehydrogenase (NAD+), GenBank Accession Number AF176568. The conceptually translated amino acid sequence of the open reading frame for the gamma-subunit of S. stercoralis isocitrate dehydrogenase (NAD+) encodes a 388 amino acid residue protein with an apparent molecular weight of 43 kDa and a predicted pI of 7.15. The sequence is 71% A/T, reflecting the characteristic A/T codon bias of S. stercoralis. The amino acid sequence of the S. stercoralis gamma-subunit of isocitrate dehydrogenase (NAD+) is compared with those of Caenorhabditis elegans, rat and human NAD(+)-ICDH. The diagnostic potential of the S. stercoralis gamma-subunit of isocitrate dehydrogenase (NAD+) is also discussed.
Siddiqui
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Department of Internal Medicine, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614, USA. siddiqui@etsu.edu
Stanley
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Berk
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D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics*; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics*; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D017171:Strongyloides stercoralis / Q000201:enzymology / Q000235:genetics*
D000947:Antigens, Helminth; D018076:DNA, Complementary; D016054:DNA, Protozoan; D016056:Immunodominant Epitopes; D007521:Isocitrate Dehydrogenase