Sister chromatid exchange in chromosomes of sheep (Ovis aries).
Blood lymphocyte cultures from 32 Comisana and Laticauda sheep breeds (15 males and 17 females) raised in Southern Italy were studied using sister chromatid exchange (SCE) test. Of the 932 cells studied, the SCE-mean value was 7.20 +/- 2.5 per cell for both breeds. Indeed, the SCE mean values were 7.12 +/- 2.45 and 7.28 +/- 2.55 in Comisana and Laticauda breeds, respectively, and the differences were not significant. No statistical differences were noticed between male and female cells (7.25 +/- 2.39 and 7.16 +/- 2.60, respectively). The SCE frequency distribution did not follow a Poisson distribution. The number of SCE were significantly higher than expected in chromosomes 1, 2 and 3 (p < 0.001) and significantly lower than expected in the X and remaining chromosomes (p < 0.001) on the basis of relative chromosome lengths.
Di Meo
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GP
National Research Council (CNR), Instituto di Ricerche Sull'Adattamento dei Bovini e dei Bufali all' Ambiente del Mezzogiorno (IABBAM), Naples, Italy.
Perucatti
A
A
Fornataro
D
D
Incarnato
D
D
Ferrara
L
L
Matassino
D
D
Iannuzzi
L
L
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D018929:Cell Culture Techniques; D002875:Chromosomes / Q000235:genetics; D005260:Female; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D012756:Sheep; D012854:Sister Chromatid Exchange / Q000235:genetics*
D001973:Bromodeoxyuridine