[Inactivation of polygalacturonase in the process of growth of the yeast Saccharomyces pastorianus].
The effect of exogenous glucose addition on polygalacturonase (EC 3.2.1.15) activity in the culture medium of Saccharomyces pastorianus was studied. An rapid but transient decrease in the enzyme activity was observed after 9-12 h after adding glucose to the culture medium. This effect was not associated with protein degradation or modification in the spectrum of secreted proteins. Ethyl acetate appeared in the culture medium during this period.
Astapovich
N I
NI
Institute of Microbiology, National Academy of Sciences of Belarus, Minsk.
Riabaia
N E
NE
D000085:Acetates / Q000032:analysis; D003470:Culture Media; D005947:Glucose / Q000008:administration & dosage; D011096:Polygalacturonase / Q000037:antagonists & inhibitors*; D012440:Saccharomyces / Q000201:enzymology / Q000254:growth & development*
D000085:Acetates; D003470:Culture Media; C007650:ethyl acetate; D011096:Polygalacturonase; D005947:Glucose