Improvement of efficiency of the Ce(IV)-induced DNA scission--relationship between the kinetic parameters (k(cat) and Km) and the DNA structure.
The Michaelis constant (Km) for double-stranded DNA, single-stranded DNA, and dinucleotide hydrolysis by Ce(IV) ion are 4.4, 15, and more than 40 mM, respectively. The order of the k(cat), however, is dinucleotide >> oligonucleotides. Not only the improvement of k(cat) but also that of Km is important for the design of an efficient artificial nuclease.
Igawa
T
T
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Japan.
Sumaoka
J
J
Komiyama
M
M
D001483:Base Sequence; D002563:Cerium; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D015226:Dinucleoside Phosphates / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*
D004277:DNA, Single-Stranded; D015226:Dinucleoside Phosphates; D009838:Oligodeoxyribonucleotides; D002563:Cerium; D004247:DNA