title: Antisense oligodeoxynucleotides: useful tool for search and assessment of new targets for anti-malarial drugs.
abstract: We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). Synthetic nuclease-resistant ODNs (phosphorothioate (PS) ODNs and ODNs containing 4'alpha-C-(2-aminoethyl)thymidines (4'-amino ODNs)) which target mitochondrial succinate dehydrogenase (SDH) iron-sulfur subunit (IP), had antimalarial activity (EC50; about 1.0 microM). Furthermore we showed that intra-parasitic SDH IP mRNA levels, which were detected using quantitative RT-PCR assay, were decreased 13% of control after the 24 h expose to SDH IP AS. From the results, we conclude that SDH has potential as the target for novel antimalarials, and AS ODNs is effective for search and assessment of targets for new antimalarial drugs.
authors.affiliation: Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ikemoto
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Kanazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ueno
authors.forename: S
authors.initials: S
authors.lastname: Shuto
authors.forename: A
authors.initials: A
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Wataya
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000737:chemistry* / Q000494:pharmacology*; D001483:Base Sequence; D015195:Drug Design; D007506:Iron-Sulfur Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology; D020319:Oligodeoxyribonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors / Q000235:genetics; D013873:Thionucleotides; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000962:Antimalarials; D007506:Iron-Sulfur Proteins; D020319:Oligodeoxyribonucleotides, Antisense; D013873:Thionucleotides; D013385:Succinate Dehydrogenase