Interaction of amphiphilic cyclic peptide with double and triple stranded DNA.
It was found that Gramicidin S (GS) with intramolecular antiparallel beta-sheet structure could bind to and thermally stabilize double and triple stranded DNA. UV melting study revealed that GS stabilized mixed sequence dsDNA in the presence of Mg2+ (deltaTm = +6.0) but it stabilized dsDNA with homosequence only in the absence of Mg2+. It should be pointed out that GS specifically stabilized the third strand (Hoogsteen base pair) but not double strand (Watson-Crick base pair) in triple helix DNA.
Yokoyama
K
K
Department of Chemistry, Kyushu School of Engineering, Kinki University, Fukuoka, Japan.
Kubo
T
T
Fujii
M
M
D020029:Base Pairing; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006096:Gramicidin / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry; D010456:Peptides, Cyclic / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012313:RNA / Q000737:chemistry; D013816:Thermodynamics; D013873:Thionucleotides
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D010456:Peptides, Cyclic; D013873:Thionucleotides; D006096:Gramicidin; D012313:RNA; D004247:DNA