title: [Preparation of T-lymphoblastoid cell lines from patients with T-cell leukemia and asymptomatic carriers of HTLV-I: analysis of the viral genome status].
abstract: Three HTLV-I-infected partially interleukin-2-dependent lymphoid cell lines were derived from a patient with T-cellular leukemia (ATL) from Georgia and a carrier of HTLV-I from Sakhalin. The strains cultured in the presence of 3-5% interleukin-2 were designated as NBK-1, NBK-2, and YE-1, respectively. Immunoblotting analysis showed typical HTLV-I proteins in them except NBK-2 which expressed nontypical proteins p40K and p28-29K. Unexpectedly, leukemic cell fraction ATL/NBK from a patient contained gag proteins p19 (CA), p24 (MA), Pr53, and unidentified protein p29. Southern blot analysis of primary leukemic cells NBK showed one full-length non-defective provirus with restriction sites Sacl in both LTRs. Limited restriction map of the provirus virtually did not differ from previously described HTLV-I prototypes. Although the mechanism of abnormal protein expression remains to be determined, this event can be explained by defective provirus formation in NBK-2 cell line during coculturing of leukemic cells with human umbilical cord blood lymphocytes.
authors.forename: O A
authors.initials: OA
authors.lastname: Pavlish
authors.forename: N B
authors.initials: NB
authors.lastname: Seniuta
authors.forename: L N
authors.initials: LN
authors.lastname: Shcherbak
authors.forename: N V
authors.initials: NV
authors.lastname: Klitsunova
authors.forename: M S
authors.initials: MS
authors.lastname: Shtutman
authors.forename: O E
authors.initials: OE
authors.lastname: Pomekhin
authors.forename: V E
authors.initials: VE
authors.lastname: Grutsevich
mesh_terms: D015139:Blotting, Southern; D016679:Genome, Viral; D015490:HTLV-I Infections / Q000097:blood* / Q000821:virology; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D015458:Leukemia, T-Cell / Q000097:blood* / Q000821:virology; D015183:Restriction Mapping; D014764:Viral Proteins / Q000235:genetics
chemical_list: D007376:Interleukin-2; D014764:Viral Proteins