title: The rug3 locus of pea encodes plastidial phosphoglucomutase.
abstract: Two cDNA clones were isolated from pea (Pisum sativum L.) and their deduced amino acid sequences shown to have significant homology to phosphoglucomutases from eukaryotic and prokaryotic sources. The longer cDNA contained a putative transit-peptide-encoding sequence, supporting the hypothesis that the isolated clones represent the cytosolic and plastidial isoforms of phosphoglucomutase in pea. Plastid protein import assays confirmed that the putative plastidial isoform was targeted to the plastid stroma where it was proteolytically processed. Expression, co-segregation, linkage, and molecular analyses have confirmed that the rug3 locus of pea encodes plastidial phosphoglucomutase. Mutations at this locus result in a near-starchless phenotype of the plant.
authors.affiliation: John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.
authors.forename: C J
authors.initials: CJ
authors.lastname: Harrison
authors.forename: R M
authors.initials: RM
authors.lastname: Mould
authors.forename: M J
authors.initials: MJ
authors.lastname: Leech
authors.forename: S A
authors.initials: SA
authors.lastname: Johnson
authors.forename: L
authors.initials: L
authors.lastname: Turner
authors.forename: S L
authors.initials: SL
authors.lastname: Schreck
authors.forename: K M
authors.initials: KM
authors.lastname: Baird
authors.forename: P L
authors.initials: PL
authors.lastname: Jack
authors.forename: S
authors.initials: S
authors.lastname: Rawsthorne
authors.forename: C L
authors.initials: CL
authors.lastname: Hedley
authors.forename: T L
authors.initials: TL
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics*; D010733:Phosphoglucomutase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018087:Plastids / Q000201:enzymology*; D012150:Polymorphism, Restriction Fragment Length; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D010733:Phosphoglucomutase