Correlation between the oscillatory and adhesion activities in erythroid cells.
The attachment kinetics of erythroid cells, such as human erythrocytes, their saponin ghosts, and erythroleukemic cells K562 to a glass surface has been studied in the presence of substances inhibiting spontaneous fluctuations of cell membranes. It has been shown that wheat germ agglutinin (WGA) slows down the attachment kinetics of K562 cells, as is the case in intact erythrocytes. Concanavalin A (Con A), which inhibits the attachment of erythrocytes to glass does not affect the adhesion of K562 cells to glass due to the absence of band 3 proteins in the membranes of K562 cells. Both lectins slow down the adhesion rate of saponin ghosts of human erythrocytes, as it takes place in intact erythrocytes. Suramin and the anionic dye ANS bind specifically to the actin protofilaments of the erythrocyte skeleton and also inhibit cell adhesion to glass. At the same time, these substances do not affect the oscillatory and adhesion activities of intact erythrocytes due to the impermeability of erythrocyte membranes for these drugs. The results obtained allow the conclusion that inhibition of erythrocyte adhesion by lectins is due to lectin binding to different constituents of the erythrocyte membrane--sialic acid moieties of glycophorin in the case of WGA and band 3 proteins in the case of Con A. The most probable mechanism of erythrocyte and K562 cell attachment to glass is the formation of the so-called local contacts between cells and the glass surface. It is also suggested that the cell surface oscillations facilitate the formation of cell contacts.
Grinfeldt
M G
MG
Institute of Cytology, Russian Academy of Sciences, St.-Petersburg.
Krol AYu
Malev
V V
VV
D001683:Biological Clocks / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism* / Q000502:physiology*; D006801:Humans; D020014:K562 Cells; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism* / Q000502:physiology*
D037102:Lectins; D008562:Membrane Glycoproteins