title: Aging, serum estradiol levels, and pregnancy differentially affect vascular reactivity of the rat uterine artery.
abstract: OBJECTIVE
  To investigate the effects of aging, ovarian ablation, and pregnancy on vascular reactivity of the rat uterine artery.
  
  
  METHODS
  Segments of uterine artery from 3-month-old pregnant and nonpregnant Wistar rats and from aged and ovariectomized animals, both 9 months of age, were exposed in vitro to vasoactive mediators. Absolute contractile force as well as endothelium-dependent and -independent vascular reactivity were determined. Isometric tension was recorded using a modified Mulvany myograph system. Results were compared with analysis of variance and Bonferroni-Dunn post hoc analysis and correlated with serum estradiol levels.
  
  
  RESULTS
  Aging up to 9 months decreased absolute tension of uterine arteries in vitro elicited by KCl (P < .0001), while not affecting receptor-operated responses to norepinephrine, endothelin-1, and angiotensin II. After ovarian ablation maximal contraction to norepinephrine was selectively reduced in the aged animal (P = .0053). Pregnancy increased absolute tension to KCl (P < .0001), norepinephrine (P < .008), and endothelin-1 (P = .0003), whereas relative contractile force (percentage of KCl) induced by norepinephrine and endothelin-1 remained unchanged and that induced by angiotension II decreased (P = .0001) in pregnant animals. In addition, pregnancy increased sensitivity to the endothelium-dependent vasodilator acetylcholine (P = .0022) but decreased that to the endothelium-independent vasodilator sodium nitroprusside (P = .0062). Endothelium-dependent relaxation correlated with serum estrogen levels remained unchanged in 9-month-old Wistar rats, which physiologically exhibited high serum estrogen concentrations but was impaired with regard to both maximum relaxation (P < .0001) and sensitivity in aged rats (P = .0007) after ovariectomy.
  
  
  CONCLUSIONS
  Vascular contractility is impaired in the uterine artery of the aged rat as evidenced by reduced responses to KCl, whereas responses to receptor-operated agonists remain unchanged. Functional ovaries are essential to preserve endothelium-dependent relaxation in aging animals. During pregnancy, contractile machinery and endothelium-dependent relaxation are enhanced. In contrast, contractions to angiotensin II and endothelium-independent relaxation to sodium nitroprusside are reduced in late pregnancy. These changes in reactivity of the uterine artery may be important for the regulation of blood flow in the uterus according to physiologic needs.
authors.affiliation: Department of Obstetrics and Gynecology and Cardiology, University Hospital Zurich, Switzerland.
authors.forename: E
authors.initials: E
authors.lastname: Wight
authors.forename: C F
authors.initials: CF
authors.lastname: Küng
authors.forename: P
authors.initials: P
authors.lastname: Moreau
authors.forename: H
authors.initials: H
authors.lastname: Takase
authors.forename: N A
authors.initials: NA
authors.lastname: Bersinger
authors.forename: T F
authors.initials: TF
authors.lastname: Lüscher
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000375:Aging / Q000097:blood*; D000818:Animals; D001158:Arteries / Q000502:physiology*; D004958:Estradiol / Q000097:blood*; D005260:Female; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D009929:Organ Size; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D014599:Uterus / Q000098:blood supply*; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects; D015430:Weight Gain
chemical_list: D004958:Estradiol; D000109:Acetylcholine; D009638:Norepinephrine