Preventive effect of antioxidant on ultraviolet-induced skin cancer in mice.
Reactive oxygen species (ROS) have been shown to be responsible for inducing DNA damage after ultraviolet radiation (UV). Antioxidant, vitamin E and epigallocatechin gallate extracted from green tea, applied topically to the skin, delayed the onset of UV-induced skin cancer in mice. Since olive oil is reported to have a potent antioxidative effect in in vitro system, we asked whether, topical use of olive oil reduces the number and delays the onset of UV-induced skin cancer in mice. We found that super virgin olive oil painted immediately after UVB radiation significantly delayed the onset and reduced the number of skin cancer, but pretreatment of super virgin olive oil and pre- and/or post treatment by regular olive oil neither retarded nor reduced skin cancer formation in UV-irradiated mice. Further, 8-hydroxy-deoxyguanosine (8-OHdG) formation in mice epidermis was apparently reduced by super virgin olive oil painted immediately after UV radiation, although cyclobutane pyrimidine dimers and (6-4) photoproducts were not reduced by olive oil treatment. Our results suggest that daily topical use of super virgin olive oil after sun bathing may delay and reduce UV-induced skin cancer development in human skin, possibly by decreasing ROS-induced 8-OHdG which is responsible for gene mutation.
Ichihashi
M
M
Department of Dermatology, Kobe University School of Medicine, 7-5-1 Kusunoki-cho Chuo-ku, Kobe, Japan. ichihash@med.kobe-u.ac.jp
Ahmed
N U
NU
Budiyanto
A
A
Wu
A
A
Bito
T
T
Ueda
M
M
Osawa
T
T
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000287:Administration, Topical; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D006801:Humans; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology; D011740:Pyrimidine Dimers / Q000378:metabolism / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012878:Skin Neoplasms / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D000069463:Olive Oil; D010938:Plant Oils; D011740:Pyrimidine Dimers; D017382:Reactive Oxygen Species; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine