title: Comparison of French natural scrapie isolates with bovine spongiform encephalopathy and experimental scrapie infected sheep.
abstract: We compared the glycoform pattern of the abnormal prion protein (PrP(Sc)) detected by immunoblotting in 21 sheep with natural scrapie, from 21 different outbreaks identified in France since 1996, with a bovine spongiform encephalopathy (BSE)-infected sheep. All the natural scrapie isolates had a higher molecular mass of the unglycosylated PrP(Sc) than in BSE-infected sheep. In the latter case, this molecular mass appeared identical to that found in the CH 1641 experimental scrapie strain (type C pattern), whereas in natural scrapie cases it was similar to that found in the SSBP/1 experimental scrapie strains. These results suggest that all French natural scrapie isolates studied so far would belong, as SSBP/1, to the group of scrapie cases with type A electrophoretic pattern.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments, 69364, Lyon, France. t.baron@lyon.afssa.fr
authors.forename: T G
authors.initials: TG
authors.lastname: Baron
authors.forename: J Y
authors.initials: JY
authors.lastname: Madec
authors.forename: D
authors.initials: D
authors.lastname: Calavas
authors.forename: Y
authors.initials: Y
authors.lastname: Richard
authors.forename: F
authors.initials: F
authors.lastname: Barillet
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism*; D005602:France / Q000453:epidemiology; D006031:Glycosylation; D008970:Molecular Weight; D018620:PrPSc Proteins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*; D012608:Scrapie / Q000453:epidemiology / Q000378:metabolism*; D012756:Sheep
chemical_list: D018620:PrPSc Proteins