The control of cyclin B1 mRNA translation during mouse oocyte maturation.
In maturing mouse oocytes, protein synthesis is required for meiotic maturation subsequent to germinal vesicle breakdown (GVBD). While the number of different proteins that must be synthesized for this progression to occur is unknown, at least one of them appears to be cyclin B1, the regulatory subunit of M-phase-promoting factor. Here, we investigate the mechanism of cyclin B1 mRNA translational control during mouse oocyte maturation. We show that the U-rich cytoplasmic polyadenylation element (CPE), a cis element in the 3' UTR of cyclin B1 mRNA, mediates translational repression in GV-stage oocytes. The CPE is also necessary for cytoplasmic polyadenylation, which stimulates translation during oocyte maturation. The injection of oocytes with a cyclin B1 antisense RNA, which probably precludes the binding of a factor to the CPE, delays cytoplasmic polyadenylation as well as the transition from GVBD to metaphase II. CPEB, which interacts with the cyclin B1 CPE and is present throughout meiotic maturation, becomes phosphorylated at metaphase I. These data indicate that CPEB is involved in both the repression and the stimulation of cyclin B1 mRNA and suggest that the phosphorylation of this protein could be involved in regulating its activity.
Tay
J
J
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
Hodgman
R
R
Richter
J D
JD
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D002453:Cell Cycle; D019926:Cyclin B / Q000378:metabolism*; D056744:Cyclin B1; D005260:Female; D017930:Genes, Reporter; D008540:Meiosis; D008677:Metaphase; D051379:Mice; D008815:Mice, Inbred Strains; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010766:Phosphorylation; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000378:metabolism
D020413:3' Untranslated Regions; C534700:Ccnb1 protein, mouse; D019926:Cyclin B; D056744:Cyclin B1; D016372:RNA, Antisense; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011061:Poly A