Co-localization of PKCepsilon with various GABA(A) receptor subunits in the mouse limbic system.
The distribution of PKCepsilon and its co-localization with various GABA(A) receptor subunits within limbic structures of the mouse brain was examined by fluorescence immunohistochemistry. Levels of PKCepsilon immunoreactivity were highest in the cingulate cortex and dentate gyrus, moderate in the nucleus accumbens, and lowest in the prelimbic cortex and basolateral amygdala. Co-localization of PKCepsilon immunoreactivity with the GABA(A) receptor alpha1, beta 2/3, and gamma2 subunits varied by subunit and brain region examined, with the majority of co-localization occuring in the dentate gyrus, nucleus accumbens and basolateral amygdala. These results demonstrate that PKCepsilon may interact with GABA(A) receptors in a subunit- and region-specific manner, and provide a potential anatomical basis for recent behavioral and biochemical evidence that PKCepsilon modulates GABA(A) receptor function.
Olive
M F
MF
Ernest Gallo Clinic and Research Center, Department of Neurology, University of California at San Francisco, Emeryville 94608, USA.
Hodge
C W
CW
D000818:Animals; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D008032:Limbic System / Q000166:cytology* / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D011493:Protein Kinase C / Q000378:metabolism*; D051744:Protein Kinase C-epsilon; D011963:Receptors, GABA-A / Q000378:metabolism*
D007527:Isoenzymes; D011963:Receptors, GABA-A; C497256:Prkce protein, mouse; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon