Molecular cloning and characterization of a novel human gene (NESCA) which encodes a putative adapter protein containing SH3.
A full-length cDNA encoding a novel protein was isolated and sequenced from a human placental cDNA library. This cDNA consists of 1990 bp and has a predicted open reading frame encoding 433 amino acids. It possesses an Src homology 3 (SH3) motif, a leucine zipper motif and no catalytic domain, suggesting that it seems to be an adapter protein. PCR-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels placed the gene to human chromosome 1q21-22.
Matsuda
S
S
Department of Molecular Pathogenesis, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Japan. smatsuda@tsuru.med.nagoya-u.ac.jp
Miyazaki
K
K
Ichigotani
Y
Y
Kurata
H
H
Takenouchi
Y
Y
Yamamoto
T
T
Nimura
Y
Y
Irimura
T
T
Nakatsugawa
S
S
Hamaguchi
M
M
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D015723:Gene Library; D005796:Genes; D006801:Humans; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D010920:Placenta / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014162:Transfection; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018076:DNA, Complementary; C410103:RUSC1 protein, human