title: Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro.
abstract: Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of myelination, and that the T4-O immunoreactive phenotype does not develop in vitro.
authors.affiliation: Department of Biomedicine and Surgery, Division of Cell Biology, Faculty of Health Sciences, University of Linköping, SE-581 85, Linköping, Sweden. emman@ibk.liu.se
authors.forename: E S
authors.initials: ES
authors.lastname: Anderson
authors.forename: C
authors.initials: C
authors.lastname: Bjartmar
authors.forename: C
authors.initials: C
authors.lastname: Eriksson
authors.forename: G
authors.initials: G
authors.lastname: Westermark
authors.forename: C
authors.initials: C
authors.lastname: Hildebrand
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D000918:Antibody Specificity; D015415:Biomarkers; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002452:Cell Count; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009836:Oligodendroglia / Q000145:classification / Q000378:metabolism*; D010641:Phenotype
chemical_list: D015415:Biomarkers; D000939:Epitopes