title: rpoS gene function is a disadvantage for Escherichia coli BJ4 during competitive colonization of the mouse large intestine.
abstract: The ability of Escherichia coli to survive stress during growth in different environments is, in large part, dependent on rpoS and the genes that comprise the rpoS regulon. E. coli BJ4 and an isogenic BJ4 rpoS mutant were used to examine the influence of the rpoS gene on E. coli colonization of the streptomycin-treated mouse large intestine. Colonization experiments in which the wild-type E. coli BJ4 and its rpoS mutant were fed individually as well as simultaneously to mice suggested that E. coli BJ4 does not face prolonged periods of nutrient starvation in the mouse large intestine and that the rpoS regulon is not expressed during long-term colonization after adaptation of the bacteria to the gut environment.
authors.affiliation: Department of Gastrointestinal Infections, Statens Serum Institut, DK 2300 Copenhagen, Denmark. KAK@ssi.dk
authors.forename: K A
authors.initials: KA
authors.lastname: Krogfelt
authors.forename: M
authors.initials: M
authors.lastname: Hjulgaard
authors.forename: K
authors.initials: K
authors.lastname: Sørensen
authors.forename: P S
authors.initials: PS
authors.lastname: Cohen
authors.forename: M
authors.initials: M
authors.lastname: Givskov
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D005260:Female; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D006361:Heating; D007420:Intestine, Large / Q000382:microbiology*; D051379:Mice; D018384:Oxidative Stress; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D002244:Carbon; D005947:Glucose