A green fluorescent protein enhancer trap screen in Drosophila photoreceptor cells.
The Drosophila ommatidia contain two classes of photoreceptor cells (PR's), the outer and the inner PR's. We performed an enhancer trap screen in order to target genes specifically expressed in PR's. Using the UAS/GAL4 method with enhanced green fluorescent protein (eGFP) as a vital marker, we screened 180000 flies. Out of 2730 lines exhibiting new eGFP patterns, we focused on 16 lines expressing eGFP in particular subsets of PR's. In particular, we describe three lines inserted near the spalt major, m-spondin and furrowed genes, whose respective expression patterns resemble those genes. These genes had not been reported to be expressed in the adult eye. These examples clearly show the ability of our screen to target genes expressed in the adult Drosophila eye.
Mollereau
B
B
Department of Biology, New York University, New York, NY 10003, USA.
Wernet
M F
MF
Beaufils
P
P
Killian
D
D
Pichaud
F
F
Kühnlein
R
R
Desplan
C
C
D000818:Animals; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005123:Eye; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics; D007763:Lac Operon; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D017956:Photoreceptor Cells, Invertebrate; D019087:Selectins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
D029721:Drosophila Proteins; D016326:Extracellular Matrix Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D008164:Luminescent Proteins; C106733:M-spondin; D019087:Selectins; D014157:Transcription Factors; C103973:fw protein, Drosophila; C085793:salm protein, Drosophila; D049452:Green Fluorescent Proteins