Toward artificial ribonucleases: design and synthesis of 2'-O-methyloligonucleotides with a terpyridine-copper(II) complex.
In order to construct an antisense 2'-O-methyloligonucleotide with an internal terpyridine-Cu(II) complex, a novel synthon was synthesized. This synthon was a 2'-deoxy-uridine-3'-phosphoramidite derivative with a terpyridine group at the 5'-O and a dimethoxytritylated hydroxypropyl group at the C-5. The antisense agent cleaved the RNA site-specifically and in a moderate yield.
Tamura
T
T
Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Furukawa
T
T
Komatsu
Y
Y
Ohtsuka
E
E
Inoue
H
H
D001483:Base Sequence; D003300:Copper; D015195:Drug Design; D007202:Indicators and Reagents; D020320:Oligoribonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry; D009942:Organometallic Compounds; D012260:Ribonucleases; D013379:Substrate Specificity; D014529:Uridine / Q000031:analogs & derivatives*
D007202:Indicators and Reagents; D020320:Oligoribonucleotides, Antisense; D009942:Organometallic Compounds; D003300:Copper; D012260:Ribonucleases; D014529:Uridine