Reduced antioxidant defense capacity in myocardial tissue from guinea pigs treated with 5-fluorouracil.
Antioxidant defense capacity was investigated in myocardial tissue from guinea pigs treated with 5-fluorouracil (5-FU) at a dose of 400 mg/kg/d daily for 5 d administered intraperitonally. Treatment with 5-FU lowered the activities of cardiac superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) accompanied by higher catalase (CAT) activity. Further, antioxidant potential (AOP) values were lower but oxidation resistance (OR) and malondialdehyde (MDA) levels were higher in the 5-FU-treated tissue. With regard to myocardial iron (Fe) and copper (Cu) levels, no significant differences were found between the groups. Results suggest that 5-FU treatment causes impairment in the myocardial antioxidant defense system and leads to cardiac peroxidation. It has been postulated that these changes might be responsible for the 5-FU cardiotoxicity seen in some patients, and antioxidant therapy might provide a therapeutic advantage.
Durak
I
I
Department of Biochemistry, Ankara University Medical Faculty, Turkey. durak@dialup.ankara.edu.tr
Karaayvaz
M
M
Kavutcu
M
M
Cimen
M Y
MY
Kaçmaz
M
M
Büyükkoçak
S
S
Oztürk
H S
HS
D000818:Animals; D000963:Antimetabolites / Q000008:administration & dosage / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000097:blood; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects; D007274:Injections, Intraperitoneal; D007501:Iron / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D000963:Antimetabolites; D000975:Antioxidants; D008315:Malondialdehyde; D003300:Copper; D007501:Iron; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005472:Fluorouracil