Comparative analysis of serological and molecular results for HLA-DR typing in 120 Thai subjects.
In this study, serological HLA-DR typing results were compared to typing results obtained with sequence-specific primers in the polymerase chain reaction (PCR-SSP). HLA-DR typing was performed on 120 random Thai individuals. Differences in HLA-DR typing results were found in 18 out of 120, which were due to cross reactive antibodies and the lack of potent antisera to define proper HLA-DR splits by serology. Furthermore, PCR-SSP is fast and easy to perform as HLA-DR typing results can be obtained within 2 hours. From the results of this study it can be concluded that PCR-SSP is a reliable and promising technique for HLA-DR typing which can replace the serological technique in routine clinical practice.
Nathalang
O
O
Department of Pathology, Pramongkutklao College of Medicine, Bangkok, Thailand.
Kupatawintu
P
P
O'Charoen
R
R
Sinsiri
S
S
Saelee
S
S
Tatsumi
N
N
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D006684:HLA-DR Antigens / Q000032:analysis*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013785:Thailand
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D006684:HLA-DR Antigens