A commercial preparation of catalase inhibits nitric oxide production by activated murine macrophages: role of arginase.
Catalase is widely used as a pharmacological probe to evaluate the role of hydrogen peroxide in antimicrobial activities of phagocytic cells. This report demonstrates that the ability of a commercial preparation of catalase to inhibit concomitantly macrophage antimycobacterial activity and production of reactive nitrogen intermediates can be attributed, at least in part, to the depletion of L-arginine by contaminating arginase. In experimental systems that employ pharmacological probes, the existence of nonspecific effects should be considered in data interpretation.
Tian
Y
Y
Departments of Medicine and Microbiology and Immunology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Xing
Y
Y
Magliozzo
R
R
Yu
K
K
Bloom
B R
BR
Chan
J
J
D000818:Animals; D001119:Arginase / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D002478:Cells, Cultured; D002956:Citrulline / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D002956:Citrulline; D009569:Nitric Oxide; D007371:Interferon-gamma; D002374:Catalase; D001119:Arginase