title: Complete nucleotide sequence of Tn10.
abstract: The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the toxic effects of various metals.
authors.affiliation: Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom. chalmers@bioch.ox.ac.uk
authors.forename: R
authors.initials: R
authors.lastname: Chalmers
authors.forename: S
authors.initials: S
authors.lastname: Sewitz
authors.forename: K
authors.initials: K
authors.lastname: Lipkow
authors.forename: P
authors.initials: P
authors.lastname: Crellin
mesh_terms: D026922:Amino Acid Transport Systems, Acidic; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D016972:Enterobacter cloacae / Q000235:genetics; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012475:Salmonella / Q000235:genetics; D012763:Shigella flexneri / Q000235:genetics*; D012964:Sodium / Q000378:metabolism; D027981:Symporters
chemical_list: D026922:Amino Acid Transport Systems, Acidic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D027981:Symporters; C066603:gltS protein, E coli; D012964:Sodium