Cleavage of parallel-stranded DNA duplex by peplomycin metal complexes.
Peplomycin-mediated degradation of parallel-stranded (ps) duplex was investigated. It was found that Co- and Fe-peplomycins degraded ps DNA duplex by 4'-hydrogen abstraction at 5'-GPy (pyrimidine) site in a similar manner to that of antiparallel B-DNA. While the orientation of two strands of ps and B-form DNA duplexes are reversed, peplomycin metal complex can bind to ps DNA duplex to cause oxidative DNA damage. These results indicate that peplomycin metal complex mainly interacts with one strand which is damaged.
Ikeda
S
S
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Japan.
Saito
I
I
Sugiyama
H
H
D001483:Base Sequence; D003035:Cobalt; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D007501:Iron; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D017663:Peplomycin / Q000737:chemistry* / Q000494:pharmacology
D009838:Oligodeoxyribonucleotides; D003035:Cobalt; D017663:Peplomycin; D004247:DNA; D007501:Iron