title: Adaptation of reptilian paramyxovirus to mammalian cells (Vero cells).
abstract: The reptilian paramyxovirus GOV was successfully adapted to Vero cells in 80 passages at 30 degrees C. The virus replicated with HA titres of 1:64 and 10(7.5) TCID50/ml in the heterologous host cells forming syncytia, giant cells and cytoplasmic inclusion bodies. After 80 passages GOV was identified by immunofluorescence, by immune electron microscopy and by PCR. Sequencing of RT-PCR products of GOV after 80 passages did not reveal changes in the nucleotide sequence of the partial L-gene of GOV.
authors.affiliation: Institute for Medical Microbiology, Infectious and Epidemic Diseases, University of Munich, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Mayr
authors.forename: J
authors.initials: J
authors.lastname: Franke
authors.forename: W
authors.initials: W
authors.lastname: Ahne
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D017812:Colubridae / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000737:chemistry; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D006386:Hemagglutination Tests / Q000662:veterinary; D016253:Microscopy, Immunoelectron / Q000662:veterinary; D018184:Paramyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D018108:Respirovirus / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D014709:Vero Cells
chemical_list: D004279:DNA, Viral