Inhibitory effects of human seminal plasma on an ELISA used to detect anti-sperm antibodies: implications for the determination of sperm quality.
The in vitro inhibitory effect of human seminal plasma on an ELISA used to detect anti-sperm antibodies have been observed. The mean inhibition rate of seminal plasma samples from 75 men was 61.5+/-23.1%. The inhibition rate of 29 samples from normal sperm group was 71. 14+/-18.25%, while that of 46 samples from the abnormal sperm group was 55.43+/-23.98%. The results show that human seminal plasma from semen with high quality sperms possesses a high inhibitory rate to anti-sperm antibody reactions, suggesting its efficiency for immunosuppression of humoral immune reactions. Its possible implications are discussed.
Lu
N Q
NQ
Jiangsu Family Planning Research Institute, Nanjing, PR China. nq-lu@public1.ptt.js.cn
Zha
S W
SW
D000328:Adult; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis* / Q000096:biosynthesis; D002452:Cell Count; D002470:Cell Survival / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006801:Humans; D008297:Male; D008875:Middle Aged; D012661:Semen / Q000276:immunology* / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D013491:Suppressor Factors, Immunologic / Q000502:physiology*
D001323:Autoantibodies; D013491:Suppressor Factors, Immunologic