Induction of nitric oxide in bovine peripheral blood mononuclear cells by bovine herpesvirus 1.
Bovine peripheral blood mononuclear cells (PBMCs) and monocytes were stimulated with bovine herpesvirus 1 (BHV-1), LPS and concanavalin A (Con A) to produce L-arginine-dependent nitric oxide (NO) in vitro. NO was detected as early as 12 hrs and up to 72 hrs post stimulation (p.s.). The NO from lipopolysacharide (LPS)-stimulated PBMCs and monocytes was found to exhibit antiviral effect against BHV-1. The anti-BHV-1 effect was inhibited with N(omega)-methyl L-arginine indicating involvement of inducible NO synthase (iNOS) in NO production.
Gupta
P K
PK
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, UP. ivri@x400.nicgw.nic.in
Saini
M
M
Gupta
L K
LK
Garg
S K
SK
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000821:virology*; D008070:Lipopolysaccharides; D009000:Monocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology; D009569:Nitric Oxide / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D009569:Nitric Oxide