A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli.
Genes for D-galactarate (gar) and D-glucarate (gud) metabolism in Escherichia coli are organized in three transcriptional units: garD, garPLRK, and gudPD. Two observations suggested a common regulator for the three operons. (i) Their expression was triggered by D-galactarate, D-glucarate, and D-glycerate. (ii) Metabolism of the three compounds was impaired by a single Tn5 insertion mapped in the yaeG gene (proposed name, sdaR), outside the D-galactarate and D-glucarate systems. Expression of the sdaR gene is autogenously regulated.
Monterrubio
R
R
Department of Biochemistry, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
Baldoma
L
L
Obradors
N
N
Aguilar
J
J
Badia
J
J
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005937:Glucaric Acid / Q000378:metabolism*; D005988:Glyceric Acids / Q000378:metabolism*; D009876:Operon; D013400:Sugar Acids / Q000378:metabolism*; D014158:Transcription, Genetic
D005988:Glyceric Acids; D013400:Sugar Acids; C042971:glyceric acid; C000090:galactaric acid; D005937:Glucaric Acid