Direct influence of melatonin on the thyroid and comparison with prolactin.
Melatonin administered in vivo had previously been shown to inhibit thyroid cell proliferation and subsequent in vitro thyroxine (T(4)) secretion in anuran tadpoles. Melatonin in vitro also directly reduced the sensitivity of the thyroid to thyrotropin (TSH). The present work sought to determine whether melatonin directly affected baseline, unstimulated T(4) secretion, and to compare its effect with that of prolactin (PRL). Thyroids from larval Rana catesbeiana or adult Rana pipiens were incubated in control or melatonin (0.01 to 100 microg/ml) media. Melatonin directly inhibited T(4) secretion by thyroids from both tadpoles and frogs at all concentrations of melatonin used and at both prometamorphic and climax tadpole stages. PRL, used in vitro at 10 microg/ml, did not influence the response of the thyroid to TSH (0.2 microg/ml) in young tadpoles, or the baseline secretion of T(4) by thyroids at any stage of larval life except climax, when T(4) secretion was significantly decreased by the third day of culture. Thus although both melatonin and PRL have been shown to antagonize the action of T(4) in vitro, and to decrease metamorphic rate, melatonin is a much more effective thyroid gland inhibitor than PRL.
Wright
M L
ML
Biology Department, College of Our Lady of the Elms, Chicopee, Massachusetts 01013, USA. wrightm@elms.edu
Cuthbert
K L
KL
Donohue
M J
MJ
Solano
S D
SD
Proctor
K L
KL
D000818:Animals; D005260:Female; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D008675:Metamorphosis, Biological / Q000187:drug effects / Q000502:physiology; D009924:Organ Culture Techniques; D011388:Prolactin / Q000494:pharmacology*; D011863:Radioimmunoassay; D011894:Rana pipiens / Q000502:physiology; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology; D013974:Thyroxine / Q000378:metabolism
D011388:Prolactin; D013972:Thyrotropin; D008550:Melatonin; D013974:Thyroxine