Homogeneous detection of a target nucleic acid sequence by combination of the intercalation activating fluorescence DNA probe and the isothermal sequence amplification.
We demonstrated the homogeneous real-time detection of RNA produced during isothermal TRC amplification by INAF probe.
Taya
T
T
Tokyo Research Laboratories, Tosoh Corporation, Kanagawa, Japan.
Saitoh
J
J
Ishizuka
T
T
Matsubayashi
K
K
Ishiguro
T
T
D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry*; D004279:DNA, Viral / Q000235:genetics; D005456:Fluorescent Dyes; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D012367:RNA, Viral / Q000032:analysis* / Q000097:blood / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004279:DNA, Viral; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D004247:DNA