Interactions between regulatory proteins that bind to the nicotinic receptor beta4 subunit gene promoter.
The genes encoding the alpha3, alpha5 and beta4 subunits of nicotinic acetylcholine receptors are tightly clustered within the genome. As these three subunits constitute the predominant acetylcholine receptor subtype expressed in the peripheral nervous system, their genomic proximity suggests a regulatory mechanism ensuring their coordinate expression. We previously identified two transcriptional regulatory elements within the beta4 promoter. One of these elements, a CT box, interacts with the regulatory factors heterogeneous nuclear ribonucleoprotein K and Puralpha. Another element, a CA box, interacts with Sp1 and Sp3. The binding site for a fifth factor, Sox10, overlaps the CT and CA boxes. As the CT and CA boxes are adjacent, we postulated that the proteins that bind to the elements interact. Here we report that the CT box-binding factors interact with each other as do the CA box-binding factors. However, there are no direct associations between the two pairs of proteins. Interestingly though, Sox10 directly interacts with all four proteins, suggesting a central role in beta4 gene expression for this member of the Sox family of regulatory factors.
Melnikova
I N
IN
Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX 78245-3207, USA.
Yang
Y
Y
Gardner
P D
PD
D017362:Cyclic AMP Response Element-Binding Protein / Q000502:physiology; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006609:High Mobility Group Proteins / Q000502:physiology*; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011978:Receptors, Nicotinic / Q000235:genetics*; D012261:Ribonucleoproteins / Q000502:physiology; D055757:SOXE Transcription Factors; D016329:Sp1 Transcription Factor / Q000502:physiology; D051705:Sp3 Transcription Factor; D014157:Transcription Factors / Q000502:physiology
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006609:High Mobility Group Proteins; C501130:PURA protein, human; D011978:Receptors, Nicotinic; D012261:Ribonucleoproteins; C527923:SOX10 protein, human; D055757:SOXE Transcription Factors; C497121:SP3 protein, human; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; C403530:nicotinic receptor alpha3beta4; D051705:Sp3 Transcription Factor; D004247:DNA