Dynamic chromatin remodeling in the vicinity of J chain gene for the regulation of two stage-specific genes during B cell differentiation.
Dynamic chromatin remodeling during B cell differentiation was identified in the vicinity of J chain gene. In pre-B cells, the enhancer-containing DNase I hypersensitive sites (HSSs) 3-4 were open. However, these HSSs 3-4 turned out to be unassociated with J chain gene expression, as the J chain promoter-containing HSS1 remained in a closed state. The open enhancer HSSs 3-4 in the pre-B cells might be related to the expression of a pre-B cell-specific gene upstream of the HSSs 3-4, which was identified in our Northern blot analyses. The HSSs 3-4 are then closed in the next immature and mature B cell stages until the IL-2 opens the HSSs 3-4 again as well as HSS1 to express J chain gene in the primary immune responses. The dynamic regulation of chromatin structure during B cell differentiation for the expression of two stage-specific genes will provide a good model system for the study of B cell differentiation and gene expression.
Kang
C J
CJ
Sensory Research Group, Creative Research Initiatives, College of Pharmacy, Seoul National University, Korea. chjkang@plaza1.snu.ac.kr
Oh
U
U
Koshland
M E
ME
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D007144:Immunoglobulin J-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013463:Sulfuric Acid Esters; D014407:Tumor Cells, Cultured
D002843:Chromatin; D007144:Immunoglobulin J-Chains; D013463:Sulfuric Acid Esters; D004247:DNA; D003850:Deoxyribonuclease I; C007482:dimethyl sulfate