Immune recognition of swine vesicular disease virus structural proteins: novel antigenic regions that are not exposed in the capsid.
Swine vesicular disease virus (SVDV) is an enterovirus of the Picornaviridae family that belongs to the coxsackievirus B group. A number of antigenic sites have been identified in SVDV by analysis of neutralizing monoclonal antibody-resistant mutants and shown to be exposed on the surface of the capsid. In this paper we have identified seven new immunodominant antigenic regions in SVDV capsid proteins by a peptide scanning method, using a panel of sera from infected pigs. When these antigenic regions were located in the capsid by using a computer-generated three-dimensional model of the virion, one was readily exposed on the surface of the virus and the remaining sites were located facing the inner side of the capsid shell, at subunit contacts, or in the interior of the subunit structure.
Jiménez-Clavero
M A
MA
Centro de Investigación en Sanidad Animal, (CISA)-INIA, Valdeolmos, Madrid, 28130, Spain.
Douglas
A
A
Lavery
T
T
Garcia-Ranea
J A
JA
Ley
V
V
D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D002213:Capsid / Q000737:chemistry / Q000276:immunology*; D003198:Computer Simulation; D029822:Enterovirus B, Human / Q000737:chemistry / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D016056:Immunodominant Epitopes / Q000276:immunology; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D019151:Peptide Library; D011487:Protein Conformation; D013552:Swine / Q000276:immunology / Q000821:virology; D013555:Swine Vesicular Disease / Q000276:immunology* / Q000821:virology*
D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D007106:Immune Sera; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D019151:Peptide Library