Effects of an anticariogenic casein phosphopeptide on calcium diffusion in streptococcal model dental plaques.
Casein phosphopeptides (CPP) stabilize amorphous calcium phosphate (ACP) and may be used to localize ACP in dental plaque, maintaining a state of supersaturation with respect to tooth enamel, reducing demineralization and enhancing remineralization. The aim here was to investigate these effects by measuring the effect of CPP-ACP on calcium diffusion in plaque. Using Dibdin's effusion system, calcium diffusion was measured in streptococcal model plaques. This demonstrated that by providing a large number of possible binding sites for calcium, 0.1% CPP-ACP reduces the calcium diffusion coefficient by about 65% at pH 7 and 35% at pH 5. Hence, CPP-ACP binds well to plaque, providing a large calcium reservoir within the plaque and slowing diffusion of free calcium. This is likely to restrict mineral loss during a cariogenic episode and provide a potential source of calcium for subsequent remineralization. Overall, once in place, CPP-ACP will restrict the caries process.
Rose
R K
RK
Division of Restorative Dentistry, The Dental School, Bristol, UK. keith.rose@bristol.ac.uk
D000704:Analysis of Variance; D002118:Calcium / Q000378:metabolism*; D002130:Calcium Phosphates / Q000494:pharmacology* / Q000627:therapeutic use; D002327:Cariostatic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002364:Caseins / Q000494:pharmacology* / Q000627:therapeutic use; D003773:Dental Plaque / Q000188:drug therapy / Q000378:metabolism*; D004058:Diffusion / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D010748:Phosphopeptides / Q000494:pharmacology* / Q000627:therapeutic use; D013290:Streptococcal Infections / Q000188:drug therapy / Q000378:metabolism*; D013295:Streptococcus mutans
D002130:Calcium Phosphates; D002327:Cariostatic Agents; D002364:Caseins; D010748:Phosphopeptides; C485828:alpha-tricalcium phosphate; C519480:amorphous calcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; D002118:Calcium