Frequent human genomic DNA transduction driven by LINE-1 retrotransposition.
Human L1 retrotransposons can produce DNA transduction events in which unique DNA segments downstream of L1 elements are mobilized as part of aberrant retrotransposition events. That L1s are capable of carrying out such a reaction in tissue culture cells was elegantly demonstrated. Using bioinformatic approaches to analyze the structures of L1 element target site duplications and flanking sequence features, we provide evidence suggesting that approximately 15% of full-length L1 elements bear evidence of flanking DNA segment transduction. Extrapolating these findings to the 600,000 copies of L1 in the genome, we predict that the amount of DNA transduced by L1 represents approximately 1% of the genome, a fraction comparable with that occupied by exons.
Pickeral
O K
OK
National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 20894, USA.
Makałowski
W
W
Boguski
M S
MS
Boeke
J D
JD
D020413:3' Untranslated Regions / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D008954:Models, Biological; D016254:Mutagenesis, Insertional / Q000235:genetics*
D020413:3' Untranslated Regions; D004247:DNA