Sonication of chitin-glucan, preparation of water-soluble fractions and characterization by HPLC.
A water-insoluble chitin-glucan complex, isolated from the mycelium of Aspergillus niger, was swollen in various aqueous media and treated subsequently by high-energy sonication. The concentration of the resulting water-soluble polysaccharide fractions was dependent on the swelling medium, the amount of the chitin-glucan complex in the suspension, and on the time of sonication. The yields of water-soluble chitin-glucan were within the range 13.6 to 24.4% relative to the mass of the original chitin-glucan. The nitrogen content obtained for the samples of water-soluble chitin-glucan indicated a higher content of chitin (3.45% of nitrogen in high-molecular fraction) than in the original water-insoluble chitin-glucan sample (1.8%). The distribution of the molecular weights of the water-soluble fractions prepared was determined by high-performance liquid chromatography.
Mislovicová
D
D
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic. mislovicova@savba.sk
Masárová
J
J
Bendzálová
K
K
Soltés
L
L
Machová
E
E
D001234:Aspergillus niger / Q000737:chemistry; D002686:Chitin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005936:Glucans / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D014462:Ultrafiltration; D014465:Ultrasonics
D005936:Glucans; D002686:Chitin