In vitro differentiation of human endometrial stromal cells: effect of transforming growth factor beta 1 on prolactin secretion.
OBJECTIVE
To investigate the role of Transforming Growth Factor beta 1 on differentiation of human endometrial stromal cells.


METHODS
A prospective study.


METHODS
Human endometrial cells obtained from 9 women were cultured in DMEM-HAMs F12 media to adequate cell confluence and then the flasks were cultured in the presence and absence of TGF b 1. The influence of TGF beta 1 were measured by prolactin production expressed as nanograms of prolactin/mg of total DNA in cells measured by diphenylamine reaction.


RESULTS
The levels of prolactin in the culture medium with and without TGF beta 1 were 0.16 +/- 0.27 ng/microgram DNA/day and 0.24 +/- 0.41 ng/microgram DNA/day respectively. There was no significant difference between groups.


CONCLUSIONS
There is no direct effect of TGF beta 1 on differentiation of human endometrial stromal cells. However it may involve in more complex relationship in this process.
Celikkanat
H
H
Dr. Zekai Tahir Burak Maternity Hospital, Ankara, Turkey.
Atac
B
B
Piskin
K
K
Helvacioglu
A
A
Gokmen
O
O
Cakar
N
N
Beksac
M S
MS
D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans; D011388:Prolactin / Q000378:metabolism*; D011446:Prospective Studies; D017154:Stromal Cells; D016212:Transforming Growth Factor beta / Q000502:physiology*
D016212:Transforming Growth Factor beta; D011388:Prolactin