Measurement of intracellular calcium levels by the fluorescent Ca(2+) indicator Calcium-Green.
The fluorescent calcium-sensitive indicators, such as the Calcium Green-1, allow one to detect small calcium transients at low indicator concentrations. The protocol reported here is a rapid and sensitive method that facilitates the measurement of intracellular free-calcium in cell suspensions. Using this assay, we were able to detect and quantify the variations in intracellular calcium concentration during microglial cell activation induced by the fragment peptides beta25-35 and PrP106-126.
Silei
V
V
Dipartimento di Biologia, Università di Roma Tre, Viale Marconi 446, 00146, Rome, Italy.
Fabrizi
C
C
Venturini
G
G
Tagliavini
F
F
Salmona
M
M
Bugiani
O
O
Lauro
G M
GM
D016229:Amyloid beta-Peptides / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism*; D017628:Microglia / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009930:Organic Chemicals; D009994:Osmolar Concentration; D010446:Peptide Fragments / Q000494:pharmacology; D011328:Prions / Q000494:pharmacology
D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D010446:Peptide Fragments; D011328:Prions; C074636:amyloid beta-protein (25-35); C082632:prion protein (106-126); C071336:calcium green; D002118:Calcium