High-performance liquid chromatography-electrospray ionization-mass spectrometry study of ginkgolic acid in the leaves and fruits of the ginkgo tree (Ginkgo biloba).
A method is developed for qualitative analysis of ginkgolic acids in the leaves and fruits of Ginkgo biloba by high-performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry technique. Negative ionization mode is successful in obtaining a very abundant deprotonated molecule [M - H]-. The mass detection sensitivity is higher than ultraviolet detection but relies heavily on the concentration of acetic acid in the HPLC eluent, which consists of acetonitrile-water-acetic acid. The method is also very specific for the analysis of ginkgolic acid with no interferences from the sample matrix.
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Research Laboratory of Natural Products Chemistry, A.M. Todd Botanicals, Eugene, OR 97402, USA.
Bernart
M W
MW
Nolan
G S
GS
Lin
L
L
Lindenmaier
M P
MP
D019342:Acetic Acid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D020441:Ginkgo biloba / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal; D012459:Salicylates / Q000032:analysis*; D012680:Sensitivity and Specificity
D010936:Plant Extracts; D012459:Salicylates; C112485:ginkgolic acid; D019342:Acetic Acid