Physical, chemical, and microbiological changes in the ceca of broiler chickens subjected to incremental feed withdrawal.
Trials were conducted to determine the effect of feed withdrawal on the weight, pH, native bacterial flora, and the persistence of Salmonella typhimurium in the ceca of market-age broilers. Broilers were provided medicated or unmedicated feed and then were subjected to feed withdrawal for 0 to 24 h in transportation crates or on litter. After feed withdrawal, broilers were stunned, bled, scalded, and picked. One cecum from each bird was aseptically removed and weighed. The cecum was then blended in 20 mL of distilled water, and the pH of the blended suspension was measured. The number of total aerobes, Enterobacteriaceae, S. typhimurium, and lactic acid bacteria in the suspension were enumerated on the appropriate bacteriological media. Results indicated that up to 24 h of feed withdrawal produced no significant change in cecal weight and that cecal pH varied by up to 0.3 units during feed withdrawal. There were significant increases in the population of Enterobacteriaceae during feed withdrawal in Trials 2 and 3, and there was a significant increase in the population of cecal aerobes in Trial 3. Feed withdrawal produced significant decreases in the population of lactic acid bacteria in all trials, but no significant change in the population of S. typhimurium occurred during feed withdrawal. There were no significant differences in cecal weight, pH, native bacteria populations, or S. typhimurium populations between broilers that were subjected to feed withdrawal on litter or in crates. Findings indicate that feed withdrawal does not always effectively evacuate the contents of the ceca and that the ceca of broilers subjected to feed withdrawal can remain a source of foodborne bacterial pathogens.
Hinton
A
A
Agricultural Research Service, United States Department of Agriculture, Russell Research Center, Athens, Georgia 30604, USA. ahinton@usda.ars.gov
Buhr
R J
RJ
Ingram
K D
KD
D000821:Animal Feed; D000818:Animals; D002432:Cecum / Q000033:anatomy & histology* / Q000737:chemistry / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology* / Q000502:physiology*; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005508:Food Deprivation; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000302:isolation & purification; D008297:Male; D012486:Salmonella typhimurium / Q000302:isolation & purification