title: Expression of SAG-1 of Toxoplasma gondii in transgenic mice.
abstract: We describe the expression of SAG-1 cDNA in B6C3F1 mice by microinjecting a 3.3 kbp DNA fragment, consisting of the cytomegalovirus enhancer-chicken beta-actin hybrid promoter and SAG-1 into the pronucleus of a fertilized egg at the one-cell stage. Offspring derived from this microinjection were analyzed for the integration and functional expression of the SAG-1 transgene. Steady-state expressions of both the mRNA for SAG-1 and SAG-1 protein product were detected in the brain, thymus, spleen and liver. Approximately 50% of F1 and F2 progeny inherited the SAG-1 transgene from SAG-1 transgenic mice in Mendelian fashion. These results indicated that SAG-1 transgenic lines were established. Transgenic mice harboring the SAG-1 gene will contribute a critical tool of defining the molecular mechanisms of SAG-1 in pathogenesis and host immune response.
authors.affiliation: The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Seng
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
authors.forename: R
authors.initials: R
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Maki
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: C
authors.initials: C
authors.lastname: Lim
authors.forename: B
authors.initials: B
authors.lastname: Zayatiin
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: X
authors.initials: X
authors.lastname: Xuan
authors.forename: I
authors.initials: I
authors.lastname: Igarashi
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: K
authors.initials: K
authors.lastname: Fujisaki
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
mesh_terms: D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D015139:Blotting, Southern; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008099:Liver / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics* / Q000378:metabolism; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D016053:RNA, Protozoan / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014122:Toxoplasma / Q000235:genetics*
chemical_list: D000953:Antigens, Protozoan; D017931:DNA Primers; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D011993:Recombinant Fusion Proteins; C047168:SAG1 antigen, Toxoplasma