Phosphorothioate G3T4G3 motifs inhibits the early stage of HIV-1 infection.
It is well known that G-rich phosphorothioate oligonucleotides (G-rich PS) bind to the V3 loop of HIV-1 gp120 and inhibit HIV-1 infection. In this study, we investigated the inhibitory mechanism of a new type of G-rich PS (PS-G3T4G3) on the replication cycle of HIV-1. PS-G3T4G3 inhibits both cell to cell and cell free infections. Binding and entry assays revealed that the inhibitory step of PS-G3T4G3 occurs at the early stage of HIV-1 infection. V3 loop-specific mAb test showed that PS-G3T4G3 binds to the V3 loop and prevents its interaction with chemokine receptors. These results suggest that PS-G3T4G3 may be a novel candidate for an HIV-1 inhibitor.
Suzuki
J
J
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Miyano-Kurosaki
N
N
Takeuchi
H
H
Tamura
Y
Y
Kawai
G
G
Takai
K
K
Tanaka
Y
Y
Tanaka
R
R
Yamamoto
N
N
Takaku
H
H
D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D002474:Cell-Free System; D015726:Giant Cells / Q000187:drug effects*; D006147:Guanine; D015699:HIV Envelope Protein gp120 / Q000187:drug effects / Q000502:physiology; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology*; D013873:Thionucleotides / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D015699:HIV Envelope Protein gp120; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D006147:Guanine