title: Crystallization and preliminary crystallographic analysis of Rieske iron-sulfur protein II (soxF) from sulfolobus acidocaldarius.
abstract: An archaeal Rieske iron-sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe--2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P6(1) or P6(5), with unit-cell parameters a = b = 80.19, c = 75.69 A. A complete data set has been collected to 1.64 A resolution at 100 K.
authors.affiliation: Karolinska Institutet, Department of Bioscience at NOVUM, Centre for Structural Biochemistry, Hälsovägen 7, S-14157 Huddinge, Sweden.
authors.forename: H
authors.initials: H
authors.lastname: Bönisch
authors.forename: C L
authors.initials: CL
authors.lastname: Schmidt
authors.forename: G
authors.initials: G
authors.lastname: Schäfer
authors.forename: R
authors.initials: R
authors.lastname: Ladenstein
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D014450:Electron Transport Complex III; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D012984:Software; D016945:Sulfolobus acidocaldarius / Q000378:metabolism*
chemical_list: D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; C037989:Rieske iron-sulfur protein; D014450:Electron Transport Complex III