Relationships between vitamin A, iron status and helminthiasis in Bangladeshi school children.
OBJECTIVE
To explore the relationships between biochemical indicators of vitamin A and iron status and the intestinal helminths Ascaris lumbricoides and hookworm in primary school children.


METHODS
Two rural governmental schools in northwestern Bangladesh.


METHODS
Cross-sectional study.


METHODS
The sample consisted of 164 children in grades 3-5.


METHODS
Serum retinol and beta-carotene (by high-performance liquid chromatography, HPLC), haemoglobin (HemoCue), ferritin (enzyme-linked immunoadsorbent assay, ELIZA) and height and weight were measured. Dietary intake of vitamin A was assessed using a food frequency questionnaire and faecal analyses were done using Stoll's egg-count technique.


RESULTS
The mean serum retinol was 26.8 microg dl(-1) and 20% had a level of < 20 microg dl(-1), the cut-off value for low vitamin A status. There was a strong positive association between serum beta-carotene and serum retinol (r = 0.44, P < 0.001), suggesting those with higher retinol levels had a higher carotene intake. Thirty-one per cent were anaemic (Hb < 11.5 g dl(-1)), 30% had iron deficiency (serum ferritin < 12.0 microg l(-1)) and 14% were suffering from iron deficiency anaemia. Children with a serum retinol level of 20 microg dl(-1) had significantly lower ferritin (14.0 compared to 26.0 microg l(-1), P = 0.005) and Hb levels (11.7 compared to 12.4 g dl(-1), P = 0.005) than those with higher levels. The proportion of iron deficiency anaemia was significantly greater among children with hookworm. Our data suggest that hookworm exerts its impact on iron status independently of the vitamin A status of the host.


CONCLUSIONS
Programmes to improve iron status should consider including both vitamin A prevention programmes and deworming.
Persson
V
V
Section for International Maternal and Child Health, Uppsala University, Sweden. viveka.persson@ich.uu.se
Ahmed
F
F
Gebre-Medhin
M
M
Greiner
T
T
D018798:Anemia, Iron-Deficiency / Q000097:blood / Q000453:epidemiology*; D000818:Animals; D001196:Ascariasis / Q000453:epidemiology*; D017164:Ascaris lumbricoides / Q000302:isolation & purification; D001459:Bangladesh / Q000453:epidemiology; D001827:Body Height; D001835:Body Weight; D002648:Child; D002664:Child Nutritional Physiological Phenomena; D002851:Chromatography, High Pressure Liquid; D003430:Cross-Sectional Studies; D004032:Diet; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005260:Female; D005293:Ferritins / Q000097:blood; D006454:Hemoglobins; D006725:Hookworm Infections / Q000453:epidemiology*; D006801:Humans; D008297:Male; D011795:Surveys and Questionnaires; D014801:Vitamin A / Q000008:administration & dosage / Q000097:blood; D014802:Vitamin A Deficiency / Q000097:blood*; D019207:beta Carotene / Q000097:blood
D006454:Hemoglobins; D019207:beta Carotene; D014801:Vitamin A; D005293:Ferritins