title: [Synthesis and immunochemical properties of the recombinant major surface glycoprotein E2 of the classical swine fever virus].
abstract: Recombinant E2 protein from vaccine strain of classical swine fever virus (CSFV) and from SCFV virulent strain Shimen was synthesized in SF-21 and High-Five cell culture with baculovirus as the expressing vector. For secretion, hydrophobic C-terminal transmembrane domain was removed and N-terminal signal polypeptide of 38 amino acids was added. Maximum accumulation of recombinant products in SF-21 cells was observed after 48 h and in medium 96 h after infection with recombinant baculovirus. In High-Five cells and in culture medium the maximum accumulation of E2 was observed after 96 h. The level of E2 expression is 5-10 micrograms/106 cells. The products of expression were purified by affinity chromatography and their specificity confirmed in immunochemical tests with a series of reference monoclonal antibodies. The product can be used for detecting antibodies to SCFV by competitive enzyme immunoassay.
authors.forename: A V
authors.initials: AV
authors.lastname: Krivonos
authors.forename: A D
authors.initials: AD
authors.lastname: Zaberezhny
authors.forename: T V
authors.initials: TV
authors.lastname: Grebennikova
authors.forename: R A
authors.initials: RA
authors.lastname: Gibadulin
authors.forename: M I
authors.initials: MI
authors.lastname: Musienko
authors.forename: V V
authors.initials: VV
authors.lastname: Tsibezov
authors.forename: V S
authors.initials: VS
authors.lastname: Bogdanova
authors.forename: S L
authors.initials: SL
authors.lastname: Kal'nov
authors.forename: T I
authors.initials: TI
authors.lastname: Aliper
authors.forename: E A
authors.initials: EA
authors.lastname: Nepoklonov
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D002846:Chromatography, Affinity; D017931:DNA Primers; D007124:Immunoenzyme Techniques; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018411:Spodoptera; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus