title: Mechanism of ribosome RNA apurinic site specific lyase.
abstract: A new enzyme, which we named ribosome RNA apurinic site specific lyase (RALyase), has been characterized. The enzyme specifically cleaves a phosphodiester bond at the apurinic site in the sarcin/ricin domain of 28S rRNA in ribosomes. The cut ends of wheat 28S rRNA were determined as 5'---GUACG-alpha-hydroxy-alpha, beta-unsaturated aldehyde and pGAGGA---3' for the 3' fragment, demonstrating that the enzyme catalyzes the beta-elimination reaction.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sawasaki
authors.forename: R
authors.initials: R
authors.lastname: Morishita
authors.forename: A
authors.initials: A
authors.lastname: Ozawa
authors.forename: T
authors.initials: T
authors.lastname: Ogasawara
authors.forename: K
authors.initials: K
authors.lastname: Madin
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
mesh_terms: D001080:Apurinic Acid; D001483:Base Sequence; D002384:Catalysis; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins; D007700:Kinetics; D037121:Plant Lectins; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012276:Ricin / Q000235:genetics; D013379:Substrate Specificity; D014908:Triticum
chemical_list: D005656:Fungal Proteins; D037121:Plant Lectins; D012339:RNA, Ribosomal, 28S; D001080:Apurinic Acid; C014801:alpha-sarcin; D012276:Ricin; D004722:Endoribonucleases; C402789:ribosomal RNA apurinic site specific lyase