title: Effect of antioxidant supplementation on the adaptive response of human skin fibroblasts to UV-induced oxidative stress.
abstract: The effect of supplementation with substances having antioxidant properties on the adaptive responses of human skin fibroblasts to UV-induced oxidative stress was studied in vitro. UVR was found to induce a substantial oxidative stress in fibroblasts, resulting in an increased release of superoxide anions and an increase in lipid peroxidation (shown by an elevated malonaldehyde content). Sub-lethal doses of UVR were also found to induce adaptive responses in the fibroblast antioxidant defences, with a transient rise in catalase and superoxide dismutase activities followed by a slower, large increase in cellular glutathione content. Supplementation of the fibroblasts with the antioxidants, Trolox (a water soluble analogue of alpha-tocopherol), ascorbic acid or beta-carotene, had differential effects on these responses. Trolox supplementation reduced the UVR-induced cellular oxidative stress and adaptive response in a predictable concentration-dependent manner. This was in contrast to ascorbic acid which increased superoxide release from fibroblasts. At low doses, ascorbate supplements also reduced the magnitude of the adaptive increases in catalase and superoxide dismutase activities and increase in glutathione content. Beta-carotene had a similar effect to ascorbic acid, reducing the extent of the adaptations to UVR at lower doses while simultaneously increasing superoxide release and malonaldehyde content. These in vitro data indicate that only the vitamin E analogue suppressed UVR-induced oxidative stress in a predictable manner and suggest that common dietary antioxidants may not be equally effective in reducing the potential deleterious effects of UVR-induced oxidative stress in skin.
authors.affiliation: Department of Medicine, University of Liverpool, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Jones
authors.forename: F
authors.initials: F
authors.lastname: McArdle
authors.forename: C I
authors.initials: CI
authors.lastname: Jack
authors.forename: M J
authors.initials: MJ
authors.lastname: Jackson
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002460:Cell Line; D002839:Chromans / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D018384:Oxidative Stress; D012867:Skin / Q000528:radiation effects*; D013481:Superoxides / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*; D019207:beta Carotene / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D002839:Chromans; D019207:beta Carotene; D013481:Superoxides; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid