Inhibition of croton oil-induced oedema in mice ear skin by capsular polysaccharides from cyanobacteria.
The anti-inflammatory properties of hydrophilic extracts of the capsular polymers of twelve cyanobacterial strains belonging to the genera Phormidium and Nostoc from marine and terrestrial habitats were tested topically on croton oil-induced oedema in mice ear skin. The screening program identified several strains as producers of anti-inflammatory products (up to 56% inhibition of the oedema). The inhibition response was dose-dependent. The application of trichloroacetic acid-treated extracts reduced the oedema by about 60%. On the other hand, one of the strains enhanced the inflammatory response. Analysis of five of the extracts showed the presence of neutral sugars (from 34.3% to 47.1%, w/w), uronic acids (from 7.1% to 26.7%, w/w) and proteins (from 30.1% to 57.0%, w/w) in the crude polymer. Rhamnose, fucose, arabinose, xylose, mannose, glucose, galactose, galacturonic acid and glucuronic acid were detected as well as sulphate groups (from 9.6% to 21.5%, w/w of sugars). The main components found were glucose and mannose.
Garbacki
N
N
Laboratoire de Pharmacognosie, Institut de Pharmacie, CHU, Tour 4, Université de Liège, Belgium. N.Garbacki@student.ulg.ac.be
Gloaguen
V
V
Damas
J
J
Hoffmann
L
L
Tits
M
M
Angenot
L
L
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D003436:Croton Oil; D000458:Cyanobacteria / Q000737:chemistry*; D004423:Ear / Q000473:pathology; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000473:pathology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011134:Polysaccharides; D003436:Croton Oil