Comparison of the distribution of magnesium in plasma determined by size exclusion chromatography and 31P NMR spectroscopy.
The distribution of magnesium in plasma, bound to proteins (pMg), complexed to low molecular weight anions (cMg) and ionized (iMg), was compared by size exclusion chromatography and an approach using a combination of atomic absorption spectroscopy, ion selective electrodes and 31P nuclear magnetic resonance spectroscopy. The distribution of pMg:cMg:iMg was 28:13:59 as determined by chromatography and 26:14:60 as determined by the latter methodology. These results are consistent with the hypothesis that plasma proteins and weak complexing anions correspond to high and low affinity magnesium binding ligands in plasma, respectively.
Batista
A
A
Department of Radiology, Ralph Rogers and Mary Nell Magnetic Resonance Center, University of Texas Southwestern Medical Center at Dallas 75235-9085, USA.
Corbett
R
R
Tidor
S
S
Castleman
E
E
Laptook
A
A
Sherry
A D
AD
D000328:Adult; D002118:Calcium / Q000097:blood; D002850:Chromatography, Gel / Q000379:methods*; D005260:Female; D006801:Humans; D008274:Magnesium / Q000097:blood*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results
D008274:Magnesium; D002118:Calcium