Photodynamic antisense regulation using psoralen-conjugated oligo(nucleoside phosphorothioate)s (I). Growth regulation of cervical carcinoma cells.
To increase the antisense regulatory effect of oligo(nucleoside phosphorothioate)s (S-Oligo), a photo-crosslinking reagent, 4, 5', 8-trimethylpsoralen, was used in this study. Psoralen-conjugated oligo(nucleoside phosphorothioate) (Ps-S-Oligo) complementary to the human papillomavirus type 18 (HPV18) mRNA drastically inhibited the cellular proliferation of cervical cancer cells only upon UVA-irradiation. In contrast, Ps-S-Oligos with mismatched sequences and scrambled one showed lesser inhibitory effects than that with matched one. These results suggest that psoralen-conjugated antisense S-Oligo has significant potential to regulate gene expression upon UVA-irradiation.
Iwase
R
R
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Yamayoshi
A
A
Nishida
J
J
Yamaoka
T
T
Wake
N
N
Murakami
A
A
D002455:Cell Division / Q000187:drug effects* / Q000528:radiation effects; D005260:Female; D011564:Furocoumarins / Q000633:toxicity*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry / Q000633:toxicity*; D027383:Papillomaviridae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013873:Thionucleotides; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms
D011564:Furocoumarins; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; D013873:Thionucleotides