Promoter elements involved in the expression of the Aspergillus parasiticus aflatoxin biosynthesis pathway gene avnA.
One of the early genes in aflatoxin biosynthesis, avnA, encodes a pathway-specific cytochrome P-450 monooxygenase that catalyzes the hydroxylation of the polyketide anthraquinone, averantin. Based on beta-glucuronidase (GUS) reporter and electrophoretic mobility shift assays, promoter sites upstream of -118 bp in the 367-bp verB-avnA intergenic region are not required for avnA gene activity. Therefore, only the -100 to -110 site of the four putative binding sites for AFLR, the aflatoxin biosynthetic pathway transcription regulatory protein (consensus binding sequence: 5'-TCGN(5)CGR-3') was required for elevated avnA expression.
Cary
J W
JW
Southern Regional Research Center, United States Department of Agriculture, PO Box 19687, 1100 R.E. Lee Blvd., New Orleans, LA, USA. jcary@nola.srrc.usda.gov
Montalbano
B G
BG
Ehrlich
K C
KC
D000348:Aflatoxins / Q000096:biosynthesis / Q000235:genetics*; D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic
D000348:Aflatoxins