Imaging the RecA-DNA complex by atomic force microscopy.
Atomic force microscopy (AFM) was applied to study the RecA protein and its complexes with DNA in air and in aqueous solution. RecA and DNA were reacted under several conditions, and deposited onto a mica substrate pre-treated in various ways. We found that the structure of the RecA and RecA-DNA complexes, especially the height of the molecules, was affected by the sample preparation method such as gel filtration, and environment during imaging.
Umemura
K
K
Joint Research Center for Atom Technology, Tsukuba, Ibaraki, Japan.
Ikawa
S
S
Nishinaka
T
T
Shibata
T
T
Kuroda
R
R
D017104:Bacteriophage M13; D004277:DNA, Single-Stranded / Q000378:metabolism / Q000648:ultrastructure*; D004279:DNA, Viral / Q000378:metabolism / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D011938:Rec A Recombinases / Q000378:metabolism / Q000648:ultrastructure*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011938:Rec A Recombinases