Interferon-gamma induces secretion of trypanosome lymphocyte triggering factor via tyrosine protein kinases.
Bidirectional signalling between Trypanosoma brucei brucei and CD8+ T cells involves reciprocal action of the parasite-derived trypanosome derived lymphocyte triggering factor (TLTF) and lymphocyte-derived IFN-gamma. Herein we further characterize this relationship and report quantitation of TLTF secretion into culture supernatants using a newly developed ELISA. Secretion is induced by IFN-gamma in a dose-response manner, with 100 U/ml giving maximal yields of bioactive TLTF as assessed by ELISA and ELISPOT. This is a constitutive and active secretion. Furthermore, IFN-gamma-induced secretion was dependent on tyrosine protein kinase activity. Specific blockers of this signalling system resulted in lower yields of TLTF in the culture supernatants.
Hamadien
M
M
Division for Infectious Diseases, Huddinge University Hospital, Sweden. Maha.Hamadien@impi.ki.se
Bakhiet
M
M
Harris
R A
RA
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007371:Interferon-gamma / Q000494:pharmacology* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D011505:Protein-Tyrosine Kinases / Q000502:physiology*; D015800:Protozoan Proteins / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D014346:Trypanosoma brucei brucei / Q000276:immunology / Q000502:physiology*; D014353:Trypanosomiasis, African / Q000469:parasitology*; D020032:Tyrphostins / Q000737:chemistry
D000911:Antibodies, Monoclonal; D004791:Enzyme Inhibitors; D015800:Protozoan Proteins; C107172:T lymphocyte triggering factor, Trypanosoma brucei; D020032:Tyrphostins; C060152:tyrphostin 47; D007371:Interferon-gamma; D011505:Protein-Tyrosine Kinases