Relationship of the substance P to indicators of host response in human gingival crevicular fluid.
BACKGROUND
The substance P (SP) level in human gingival crevicular fluid (GCF) was studied in relation to clinical periodontal variables and to various indicators of host response in the GCF.


METHODS
GCF was collected from periodontal sites with gingival inflammation and shallow or moderately deep pocket in 48 subjects. The total amount of SP and the substances based on host response factors in a 30-s sample were determined by ELISA and enzymatic methods.


RESULTS
Significant correlation was found between SP and probing depth (r= 0.637, p<0.001), while correlation was weak between SP and either gingival (r= 0.177, p=0.23) or plaque index (r=0.008, p=0.96). SP also showed significant correlation with the indicators of host response: prostaglandin E2, aspartate aminotransferase, alkaline phosphatase, myeloperoxidase, interleukin-1beta, tumor necrosis factor-alpha, interleukin-8 and monocyte chemoattractant protein-1 (r=0.434-0.867, p<0.01-0.001).


CONCLUSIONS
These results indicate that neuropeptide SP in GCF may have a potential as an indicator of periodontal inflammation and the host response.
Hanioka
T
T
Department of Preventive Dentistry, Osaka University Faculty of Dentistry, Japan. haniokat@dent.osaka-u.ac.jp
Takaya
K
K
Matsumori
Y
Y
Matsuse
R
R
Shizukuishi
S
S
D000469:Alkaline Phosphatase / Q000032:analysis; D001219:Aspartate Aminotransferases / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D018932:Chemokine CCL2 / Q000032:analysis; D003774:Dental Plaque Index; D015232:Dinoprostone / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000201:enzymology / Q000276:immunology; D005891:Gingivitis / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D010518:Periodontitis / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D009195:Peroxidase / Q000032:analysis; D013373:Substance P / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D015415:Biomarkers; D018932:Chemokine CCL2; D007375:Interleukin-1; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P; D009195:Peroxidase; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D015232:Dinoprostone