title: Protein turnover modifications induced by the protein feeding pattern still persist after the end of the diets.
abstract: This study was undertaken to determine whether the protein feeding pattern could induce chronic adaptation of protein turnover. After a 15-day adaptive period, elderly (68 yr) and young (26 yr) women received, for 14 days, a diet providing 200 KJ x kg fat-free mass (FFM)(-1) x day(-1), where the daily protein intake (1.7 g protein x kg FFM(-1) x day(-1)) was either spread over 4 meals in the spread pattern or mainly (80%) consumed at noon in the pulse pattern. One day after the end of the dietary treatment, whole body leucine kinetics were measured by use of a continuous [(13)C]leucine infusion, both in the postabsorptive state and in the same fed state. The pulse pattern was able to induce, in young as in elderly women, a lower postabsorptive leucine oxidation and endogenous leucine flux than the spread pattern and improved the responsiveness of nonoxidative leucine disposal during 4-h oral feeding. Thus the pulse pattern was able to induce chronic regulation of protein metabolism in young as in elderly women.
authors.affiliation: Unité d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique et Centre de Recherche en Nutrition Humaine, 63122 Clermont-Ferrand-Theix, France. arnalma@clermont.inra.fr
authors.forename: M A
authors.initials: MA
authors.lastname: Arnal
authors.forename: L
authors.initials: L
authors.lastname: Mosoni
authors.forename: Y
authors.initials: Y
authors.lastname: Boirie
authors.forename: P
authors.initials: P
authors.lastname: Gachon
authors.forename: M
authors.initials: M
authors.lastname: Genest
authors.forename: G
authors.initials: G
authors.lastname: Bayle
authors.forename: J
authors.initials: J
authors.lastname: Grizard
authors.forename: M
authors.initials: M
authors.lastname: Arnal
authors.forename: J M
authors.initials: JM
authors.lastname: Antoine
authors.forename: B
authors.initials: B
authors.lastname: Beaufrère
authors.forename: P P
authors.initials: PP
authors.lastname: Mirand
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D000368:Aged; D000375:Aging; D001639:Bicarbonates; D001786:Blood Glucose / Q000032:analysis; D002247:Carbon Isotopes; D003903:Deuterium; D004032:Diet; D004044:Dietary Proteins / Q000008:administration & dosage*; D002149:Energy Intake; D005260:Female; D005502:Food; D006801:Humans; D007328:Insulin / Q000097:blood; D007700:Kinetics; D007930:Leucine; D011506:Proteins / Q000378:metabolism*
chemical_list: D001639:Bicarbonates; D001786:Blood Glucose; D002247:Carbon Isotopes; D004044:Dietary Proteins; D007328:Insulin; D011506:Proteins; D003903:Deuterium; D007930:Leucine