Determination of phenytoin in human plasma by gas chromatography.
A gas chromatographic method for the determination of phenytoin in plasma is described. This assay allows the determination of phenytoin for therapeutic drug monitoring with a minimum detectable limit of 200 ngmL-1 for 500 mL plasma. Separation was performed on 2 m x 2 mm i.d. 1.5% OV17, 1.95 OV210 packed column using a flame-ionization detector. Barbital and tetramethyl ammonium hydroxide were used as internal standard and derivatizing agent, respectively. This method is simple, rapid and suitable for routine analysis of phenytoin in plasma.
Zarghi
A
A
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
Gholami
K
K
Hessami
M
M
D000927:Anticonvulsants / Q000097:blood*; D002849:Chromatography, Gas; D006801:Humans; D010672:Phenytoin / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results
D000927:Anticonvulsants; D010672:Phenytoin