Characterization of a major envelope protein from the rumen anaerobe Selenomonas ruminantium OB268.
Cell envelopes from the Gram-negative staining but phylogenetically Gram-positive rumen anaerobe Selenomonas ruminantium OB268 contained a major 42 kDa heat modifiable protein. A similarly sized protein was present in the envelopes of Selenomonas ruminantium D1 and Selenomonas infelix. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of Triton X-100 extracted cell envelopes from S. ruminantium OB268 showed that they consisted primarily of the 42 kDa protein. Polyclonal antisera produced against these envelopes cross-reacted only with the 42 kDa major envelope proteins in both S. ruminantium D1 and S. infelix, indicating a conservation of antigenic structure among each of the major envelope proteins. The N-terminus of the 42 kDa S. ruminantium OB268 envelope protein shared significant homology with the S-layer (surface) protein from Thermus thermophilus, as well as additional envelope proteins containing the cell surface binding region known as a surface layer-like homologous (SLH) domain. Thin section analysis of Triton X-100 extracted envelopes demonstrated the presence of an outer bilayer over-laying the cell wall, and a regularly ordered array was visible following freeze-fracture etching through this bilayer. These findings suggest that the regularly ordered array may be composed of the 42 kDa major envelope protein. The 42 kDa protein has similarities with regularly ordered outer membrane proteins (rOMP) reported in certain Gram-negative and ancient eubacteria.
Kalmokoff
M L
ML
Centre for Food and Animal Research, Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
Austin
J W
JW
Whitford
M F
MF
Teather
R M
RM
D000595:Amino Acid Sequence; D000693:Anaerobiosis; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017830:Octoxynol / Q000737:chemistry; D012417:Rumen / Q000382:microbiology*; D020357:Selenomonas / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D017830:Octoxynol