Effects of ion mode and matrix additives in the identification of bacteria by intact cell mass spectrometry.
Protocols for the identification of bacterial cells by intact cell matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (ICM-TOFMS) are presented. A mass range of 500 to 10,000 m/z is used. The use of formic acid and the crown ether 1, 4, 7, 10, 13, 16-hexaoxacyclooctadecane (18-crown-6) is described. Crown ether is useful for removing metal ion adducts, which degrade spectral purity, and formic acid promotes positive ions, improves spectral signal, and, hence, increases identification certainty.
Evason
D J
DJ
Bio-analytical Research Centre, Manchester Metropolitan University, UK.
Claydon
M A
MA
Gordon
D B
DB
D001419:Bacteria / Q000737:chemistry* / Q000145:classification*; D043844:Crown Ethers; D016983:Enterococcus / Q000737:chemistry; D004988:Ethers, Cyclic / Q000737:chemistry; D005561:Formates / Q000737:chemistry; D013058:Mass Spectrometry; D008670:Metals / Q000737:chemistry; D012964:Sodium / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014465:Ultrasonics
D043844:Crown Ethers; D004988:Ethers, Cyclic; D005561:Formates; D008670:Metals; C030544:formic acid; C015762:18-crown-6; D012964:Sodium