title: Characteristic expression of thrombomodulin in the muscle sarcoplasm in patients with the acute phase of rhabdomyolysis.
abstract: The expression of thrombomodulin and neural cell adhesion molecule (NCAM) was studied immunocytochemically in biopsied muscle specimens from 10 patients with rhabdomyolysis with different etiologic factors, including 5 with malignant hyperthermia. We have already reported that thrombomodulin was expressed on regenerating muscle cell membranes as well as on vessel walls in patients with various neuromuscular diseases, including Duchenne muscular dystrophy, Becker muscular dystrophy and inflammatory myopathy. We found increased expression of thrombomodulin not only on the sarcolemma, but also in the sarcoplasm of a fair number of muscle fibers in the acute phase of rhabdomyolysis. The granular pattern of thrombomodulin expression in the sarcoplasm seems to be a characteristic finding in the acute phase of rhabdomyolysis. Most muscle fibers which expressed NCAM on the sarcolemma also expressed thrombomodulin. However, the muscle fibers which expressed thrombomodulin in the sarcoplasm did not express NCAM, and showed a degenerative appearance on electron microscopic examination. These results suggest that thrombomodulin is expressed in the sarcoplasm during the acute degeneration phase of rhabdomyolysis in addition to the expression on the sarcolemma during the muscle fiber regeneration as shown in our previous study, and the former process, which is characterized by the granular expression of thrombomodulin in the sarcoplasm, may be a characteristic finding in rhabdomyolysis.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.forename: I
authors.initials: I
authors.lastname: Higuchi
authors.forename: M
authors.initials: M
authors.lastname: Inose
authors.forename: T
authors.initials: T
authors.lastname: Niiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Uchida
authors.forename: M
authors.initials: M
authors.lastname: Osame
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D003593:Cytoplasm / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008305:Malignant Hyperthermia / Q000473:pathology; D008854:Microscopy, Electron; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000473:pathology*; D019006:Neural Cell Adhesion Molecules / Q000032:analysis*; D009468:Neuromuscular Diseases / Q000473:pathology*; D012206:Rhabdomyolysis / Q000473:pathology*; D012508:Sarcolemma / Q000473:pathology; D018180:Thrombomodulin / Q000032:analysis*
chemical_list: D019006:Neural Cell Adhesion Molecules; D018180:Thrombomodulin