Isolation of antigen from the circulating immune complex in mice infected with Plasmodium berghei.
Circulating immune complex (CIC) is known to play a role in pathological glomerular alterations in malaria. However, the nature of the antigens comprising the CIC is still not fully understood. We report here the isolation of the antigen in CIC and its localisation in mice infected with Plasmodium berghei NK65. The antigen was successfully isolated from CIC extracted from the blood of mice infected with P. berghei, by using C1q-coated microplates. The molecular mass of the antigen separated from CIC bound to C1q was found to be 78 kDa. Furthermore, localisation of the antigen was examined by the fluorescent antibody technique and immunoelectron microscopy. The antigen was detected in the parasitised erythrocyte and the mesangial matrix by both methods. These results suggest that the 78 kDa protein might be associated with the glomerular alterations in malaria infection.
Kusuhara
Y
Y
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan. kusuhara@fujita-hu.ac.jp
Maeno
Y
Y
Nagase
K
K
Sakai
K
K
Nakazawa
S
S
Kanbara
H
H
Taniguchi
K
K
Nakabayashi
T
T
D000818:Animals; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D008288:Malaria / Q000276:immunology*; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan