N-Bromoacetylethanolamine phosphate as a probe for the identification of a liver microsomal glucose-6-phosphate transporter peptide in rats and Ehrlich ascites tumor-bearing mice.
Hepatic microsomal glucose-6-phosphatase is a multicomponent system composed of substrate/product translocases and a catalytic subunit. Previously we (Foster et al. (1996) Biochim. Biophys. Acta 12, 244-254) demonstrated that N-bromoacetylethanolamine phosphate (BAEP) is a time-dependent, irreversible inhibitor of glucose-6-phosphate hydrolysis in intact but not disrupted microsomes. We proposed that BAEP manifests its inhibitory effect by binding with a glucose-6-phosphate translocase protein of the glucose-6-phosphatase system. Here we provide additional evidence that BAEP inhibits glucose-6-phosphate transport in microsomal vesicles and utilize [(32)P]BAEP as an affinity label in the identification of a glucose-6-phosphate transport protein. In this study, we identify 51-kDa rat and mouse liver microsomal proteins involved in glucose-6-phosphate transport into and out of microsomal vesicles by utilizing (1) an Ehrlich ascites tumor-bearing mouse model, which displays a decreased sensitivity to the time-dependent inhibitory effect of BAEP, and (2) another glucose-6-phosphate translocase inhibitor, tosyl-lysine chloromethyl ketone, in conjunction with [(32)P]BAEP as an affinity label.
Foster
J D
JD
Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, USA.
Stevens
A L
AL
Nordlie
R C
RC
D000345:Affinity Labels / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D017920:Antiporters / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism* / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D051381:Rats; D013997:Time Factors; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology
D000345:Affinity Labels; D017920:Antiporters; D011756:Diphosphates; D004983:Ethanolamines; D009004:Monosaccharide Transport Proteins; D010455:Peptides; C503532:Slc37a4 protein, mouse; C503533:Slc37a4 protein, rat; C111261:glucose 6-phosphate(transporter); D014107:Tosyllysine Chloromethyl Ketone; C020161:N-bromoacetylethanolamine phosphate; D019298:Glucose-6-Phosphate; D005952:Glucose-6-Phosphatase