Rapid purification of fumonisins and their hydrolysis products with solid-phase extraction columns.
Fumonisins B(3) and B(4) (FB(3) and FB(4)) were recovered from the 50:50 acetonitrile/water extract of corn cultures of a strain of Fusarium moniliforme that does not make FB(1) or FB(2) by stirring the extract with IRA-68, a weak anion-exchange resin. The fumonisins were desorbed with 5% acetic acid in the same solvent. After dilution with water, the desorbed fumonisins were separated into FB(3) (FB(3) and FA(3)) and FB(4) (FB(4), FC(4), and FA(4)) fractions with a tC(18) solid-phase extraction (SPE) cartridge. The FB(3) fraction was then separated into FB(3) and FA(3) by using an NH(2) SPE cartridge and eluting with 5% acetic acid and increasing amounts of acetonitrile in water. Finally, FB(1) and FA(3) were hydrolyzed with calcium hydroxide. After recovery from the reaction mixture using a tC(18) cartridge, the hydrolyzed and partially hydrolyzed analogues were separated and the unreacted fumonisins recovered by using an NH(2) cartridge, initially in the normal-phase mode with increasing amounts of water in acetonitrile and then in the reversed-phase mode after the addition of 5% acetic acid to the solvent and eluting in the reverse order.
Poling
S M
SM
Mycotoxin Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL 61604, USA. polingsm@mail.ncaur.usda.gov
Plattner
R D
RD
D002264:Carboxylic Acids / Q000737:chemistry* / Q000302:isolation & purification; D002274:Carcinogens, Environmental / Q000737:chemistry* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000379:methods; D037341:Fumonisins; D005670:Fusarium / Q000737:chemistry*; D006868:Hydrolysis; D009183:Mycotoxins / Q000737:chemistry* / Q000302:isolation & purification; D003313:Zea mays / Q000382:microbiology*
D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D037341:Fumonisins; D009183:Mycotoxins; C073063:fumonisin B3; C076287:fumonisin B4