Surface antigen, SpaA, of erysipelothrix rhusiopathiae binds to Gram-positive bacterial cell surfaces.
In a previous study, we isolated the spaA gene encoding the surface protective antigen A, SpaA, of Erysipelothrix rhusiopathiae, and found that the N-terminal region of SpaA was responsible for protective immunity against erysipelas and that the C-terminal region contained eight repeat units consisting of 20 amino acids comprising the binding domain on the Erysipelothrix cell surface. In this study, using recombinant SpaA proteins, we showed that the repeat region bound to the cell surfaces of various Gram-positive bacterial cells, SpaA was a membrane-associated protein, this association depended on the interaction with choline residues in teichoic acid, and SpaA bound to lipoteichoic acid (LTA) of Bacillus subtilis and Staphylococcus aureus. These results showed that LTA was required for the surface association of SpaA in E. rhusiopathiae and that such an association might be common among Gram-positive bacterial cells. We suggested that an LTA-SpaA complex might have an important role in the E. rhusiopathiae infection process.
Makino
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SI
Department of Veterinary Microbiology, University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan. smakino@obihiro.ac.jp
Yamamoto
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K
Asakura
H
H
Shirahata
T
T
D000942:Antigens, Bacterial / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins; D001665:Binding Sites; D002462:Cell Membrane / Q000502:physiology; D003001:Cloning, Molecular; D004888:Erysipelothrix / Q000235:genetics / Q000502:physiology*; D006094:Gram-Positive Bacteria / Q000502:physiology*; D008070:Lipopolysaccharides / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013682:Teichoic Acids / Q000378:metabolism
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C114457:SpaA protein, Erysipelothrix rhusiopathiae; D013682:Teichoic Acids; C009900:lipoteichoic acid