Experimental autoimmune myositis in the lewis rat: lack of spontaneous T-cell apoptosis and therapeutic response to glucocorticosteroid application.
Recently, it has been shown that inflammatory T-cells in human idiopathic myositis only very rarely undergo spontaneous apoptosis. The animal model of experimental autoimmune myositis (EAM) in the Lewis rat was chosen to investigate whether similar findings hold true in rat muscle and if glucocorticosteroids act by induction of T-cell apoptosis in inflammatory lesions. The rate of spontaneous T-cell apoptosis in rat EAM was low, even in muscle specimens with extensive inflammation. After intravenous glucocorticosteroid pulse therapy we found a dramatic increase in the rate of apoptotic T-cells in the inflamed muscles. Up to 50% of these apoptotic T-cells were CD8 positive apoptotic T-cells. T-cell apoptosis was significantly lower in similarly inflamed muscle specimens of the control group. We suggest that glucocorticosteroids induce apoptosis of endomysial T-cells in human idiopathic polymyositis. Glucocorticosteroid-induced apoptosis may be a candidate mechanism in the termination of inflammatory activity.
Schneider
C
C
Department of Neurology, Julius-Maximilians-Universität Würzburg, Josef-Schneider-Strasse 11, 97080, Würzburg, Germany.
Matsumoto
Y
Y
Kohyama
K
K
Toyka
K V
KV
Hartung
H P
HP
Gold
R
R
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology / Q000502:physiology; D005938:Glucocorticoids / Q000627:therapeutic use*; D007275:Injections, Intravenous; D008775:Methylprednisolone / Q000627:therapeutic use*; D009132:Muscles / Q000473:pathology / Q000503:physiopathology; D020721:Nervous System Autoimmune Disease, Experimental / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology*
D005938:Glucocorticoids; D008775:Methylprednisolone