Isolation of emetine resistant clones of Entamoeba histolytica by petri dish agar method.
Emetine resistant clones of Entamoeba histolytica strain HM1:IMSS were isolated by using petri dish agar method after mutation with ethyl-methanesulphonate. Two emetine resistant clones were obtained and both were resistant to emetine at a concentration of 24 micrograms/ml of emetine. The 50 per cent inhibitory concentration (IC50) for both emetine sensitive and resistant clones was 5 and 14 micrograms/ml respectively. The colony forming efficiency of E. histolytica strain HM1:IMSS varied from 44 to 54 per cent. This method is useful for isolating clones from different strains of the parasite for molecular and immunological studies.
Prabhu
R
R
Department of Parasitology, Postgraduate Institute of Medical Education & Research, Chandigarh.
Sehgal
R
R
Chakraborti
A
A
Malla
N
N
Ganguly
N K
NK
Mahajan
R C
RC
D000362:Agar; D000563:Amebicides / Q000494:pharmacology*; D000818:Animals; D004352:Drug Resistance, Microbial / Q000235:genetics; D004640:Emetine / Q000494:pharmacology*; D004748:Entamoeba histolytica / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008828:Microbiological Techniques
D000563:Amebicides; D000362:Agar; D004640:Emetine