title: An in vitro model of ischemic-like stress in retinal pigmented epithelium cells: protective effects of antioxidants.
abstract: We have developed a model of in vitro cell oxidative stress in bovine retinal pigment epithelium cells exposed to a ischemia-like condition obtained by interference with glucose utilization through both oxidative phosphorylation and glycolysis. This resulted in a statistically significant decrease of the intracellular ATP levels, which reflects a bioenergetic decline similar to that associated with mitochondrial damage or loss in normal post-mitotic cells aging in vivo. This new model of cellular oxygen stress seems adequate for investigation of the protective action of antioxidants, in agreement with our finding of a statistically significant increase in the ATP levels over the values of the non-treated samples in retinal pigment epithelium cells exposed to the above oxygen stress in medium supplemented with 300 microM vitamin C or 10 mM N-acetylcysteine.
authors.affiliation: Department I of Optics, University of Alicante, Aptdo. 99, E-3080, Alicante, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Palmero
authors.forename: J L
authors.initials: JL
authors.lastname: Bellot
authors.forename: M
authors.initials: M
authors.lastname: Castillo
authors.forename: C
authors.initials: C
authors.lastname: García-Cabanes
authors.forename: J
authors.initials: J
authors.lastname: Miquel
authors.forename: A
authors.initials: A
authors.lastname: Orts
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D007425:Intracellular Membranes / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012171:Retinal Vessels
chemical_list: D000975:Antioxidants; D000255:Adenosine Triphosphate