title: Immunoelectron microscope analysis of epidermal growth factor receptor (EGFR) in isolated Mytilus galloprovincialis (Lam.) digestive gland cells: evidence for ligand-induced changes in EGFR intracellular distribution.
abstract: In mammalian cells, the binding of epidermal growth factor (EGF) to its receptor (EGFR), a glycoprotein with intrinsic tyrosine kinase activity, leads to the pleiotropic responses to EGF. Among these, a negative feedback response by stimulation of receptor internalization and lysosomal degradation, this attenuating signal transduction. In this work, data are reported on the identification of specific EGFRs in isolated digestive gland cells from the marine mussel (Mytilus galloprovincialis Lam.) By immunoelectron microscopy. In control digestive cells, EGFR immunoreactivity was mainly associated with cytoplasmic membrane structures and, to a lesser extent, the cell membrane. The presence of EGFR-like receptors was confirmed by Western blotting of digestive gland cell extracts with two different monoclonal antibodies that recognize either intracellular or extracellular epitopes. The addition of mammalian EGF resulted in significant time and temperature-dependent changes in EGFR subcellular distribution in mussel cells. In cells exposed to EGF for 0-15 min at 4 degrees C, the distribution of EGFR was not significantly different from that of the control cells. On the other hand, at 18 degrees C, an increased labelling along the cell membrane was observed after 5-10 min after EGF addition, with a concomitant decrease in the cytoplasmic signal. Moreover, after 20 min of exposure to EGF, ligand binding apparently resulted in EGFR compartmentation within the lysosomes. These observations were confirmed by quantitative analysis of EGFR labelling at different times of EGF exposure. Similar results were obtained utilizing the two different monoclonal antibodies. The results indicate that, in mussel digestive cells, the binding of heterologous EGF to specific receptors induces a negative feedback response by stimulating the lysosomal degradation of EGFR, thus suggesting the presence of mechanisms responsible for receptor downregulation similar to those observed in mammalian cells.
authors.affiliation: Istituto di Scienze Fisiologiche, Loc. Crocicchia, Urbino, Italy. canesi@uniurb.it
authors.forename: L
authors.initials: L
authors.lastname: Canesi
authors.forename: M
authors.initials: M
authors.lastname: Malatesta
authors.forename: S
authors.initials: S
authors.lastname: Battistelli
authors.forename: C
authors.initials: C
authors.lastname: Ciacci
authors.forename: G
authors.initials: G
authors.lastname: Gallo
authors.forename: G
authors.initials: G
authors.lastname: Gazzanelli
mesh_terms: D000818:Animals; D001665:Binding Sites; D049872:Bivalvia / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D004068:Digestive System Physiological Phenomena; D015536:Down-Regulation; D066246:ErbB Receptors / Q000378:metabolism / Q000502:physiology*; D008024:Ligands; D051379:Mice; D016253:Microscopy, Immunoelectron
chemical_list: D008024:Ligands; D066246:ErbB Receptors