Methionine-induced phytoalexin production in rice leaves.
The application of methionine on wounded rice leaves induced the production of rice phytoalexins, sakuranetin and momilactone A. This induction resulted from stimulation of phenylalanine ammonia-lyase and naringenin 7-O-methyltransferase activity. Jasmonic acid, ethylene, and active oxygen species are important as signal transducers in disease resistance mechanisms. However, although the endogenous level of jasmonic acid rapidly increased in reaction to wound, methionine treatment could not induced endogenous JA production. Ethylene induced the production of the flavonoid phytoalexin, sakuranetin, but did not induce the production of a terpenoid phytoalexin, momilactone A. On the other hand, a free radical scavenger, Tiron, counteracted the induction of both sakuranetin and momilactone A production in methionine-treated leaves. Active oxygen species may be important in methionine-induced production of phytoalexins.
Nakazato
Y
Y
Laboratory of Phytochemical Ecology, School of Agriculture, Ibaraki University, Ami, Japan.
Tamogami
S
S
Kawai
H
H
Hasegawa
M
M
Kodama
O
O
D003517:Cyclopentanes / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism; D005030:Ethylenes; D005419:Flavonoids / Q000096:biosynthesis / Q000737:chemistry; D016166:Free Radical Scavengers; D008715:Methionine / Q000378:metabolism*; D015394:Molecular Structure; D012275:Oryza / Q000378:metabolism*; D054883:Oxylipins; D010936:Plant Extracts / Q000096:biosynthesis*; D018515:Plant Leaves / Q000378:metabolism; D012717:Sesquiterpenes; D013729:Terpenes; D000094162:Phytoalexins
D003517:Cyclopentanes; D004224:Diterpenes; D005030:Ethylenes; D005419:Flavonoids; D016166:Free Radical Scavengers; D054883:Oxylipins; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; C099724:sakuranetin; C116023:momilactone A; C011006:jasmonic acid; C036216:ethylene; D008715:Methionine; D000094162:Phytoalexins