title: Adsorption of endotoxin on glass in the presence of rhIL-11.
abstract: Poor recovery of spiked endotoxin in the Limulus amebocyte lysate assay (LAL assay) was observed in the presence of recombinant human interleukin-11 (rhIL-11), a cationic, hydrophobic protein. Detection of endotoxin activity remaining in the empty glass tubes in which endotoxin and rhIL-11 mixtures were incubated indicated adsorption of endotoxin on glass. At low concentrations of rhIL-11, a correlation between endotoxin adsorbed on glass and a decrease of endotoxin in solution was observed. Adsorption of rhIL-11 on glass correlated with adsorption of endotoxin, which indicates that rhIL-11 mediates adsorption of endotoxin on glass. Consequently, adsorption of endotoxin on glass may occur in the presence of other substances which bind to both of endotoxin and glass.
authors.affiliation: Yamanouchi Pharmaceutical Company Limited, Tsukuba-shi, Japan. yokota_h@yamanouchi.co.jp
authors.forename: H
authors.initials: H
authors.lastname: Yokota
authors.forename: H
authors.initials: H
authors.lastname: Kiyonaga
authors.forename: H
authors.initials: H
authors.lastname: Kaniwa
authors.forename: N
authors.initials: N
authors.lastname: Saisho
mesh_terms: D000327:Adsorption; D004731:Endotoxins / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D017370:Interleukin-11 / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D004731:Endotoxins; D017370:Interleukin-11; D011994:Recombinant Proteins