[An evaluation of a nutrient medium for isolating and cultivating Helicobacter pylori].
Selective Helicobacter agar containing the selective supplement and blood, adding ex tempore, for the isolation and cultivation of H. pylori was developed. The Helicobacter agar was studied with the use of 5 newly isolated H. pylori strains, 13 bacterial associated cultures, as well as 21 inoculated biopsy specimens of the gastric and duodenal mucosa of patients with peptic ulcer. The study revealed that Helicobacter agar ensured the growth of H. pylori and their isolation from clinical material. The positive results after the inoculation of the specimens of biopsy material on Helicobacter agar and control media was 85%. In addition, the study of Helicobacter agar showed that it also exhibited pronounced selective properties with respect to bacterial associations, not inhibiting the growth of Helicobacter organisms and retaining their main biological properties. It is possible to recommend Helicobacter agar for use in laboratory practice in diagnosing Helicobacter-associated diseases.
Medzhidov
M M
MM
Research and Production Amalgamation Nutrient Media, Republican Clinical Hospital, Makhachkala, Russia.
Temirkhanova
Z U
ZU
Alieva
Kh M
KhM
Isakhanov
B M
BM
D000362:Agar; D001706:Biopsy; D015169:Colony Count, Microbial; D003470:Culture Media; D004381:Duodenal Ulcer / Q000382:microbiology; D004386:Duodenum / Q000382:microbiology / Q000473:pathology; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D013276:Stomach Ulcer / Q000382:microbiology
D003470:Culture Media; D000362:Agar