Chemoenzymatical grafting of acrylamide onto lignin.
Laccase (E.C.1.10.3.2.) from white-rot basidiomycete Trametes versicolor and dioxane peroxides were essential in the copolymerization of acrylamide and a derivative with lignin in a dioxane-H(2)O (7:3) mixture. Both a solubility test and an elemental analysis of the eluted and separated fractions provided evidence of grafting. Gel permeation chromatography on Sephadex G-100 showed that the side chain and the lignin backbone migrate as one unit through the column. Copolymers obtained were characterized by UV-VIS-spectroscopy as well as FT-IR, and 13C-CPMAS-NMR spectroscopy. Freeze-dried copolymers of lignin and acrylamide appeared as homogeneous fibril-like particulate. The mechanism of the enzymatical grafting is discussed.
Mai
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Institut für Forstbotanik der Universität Göttingen, Büsgenweg 2, D-37077, Göttingen, Germany. cmai@gwdg.de
Milstein
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Hüttermann
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D020106:Acrylamide / Q000737:chemistry*; D001487:Basidiomycota / Q000201:enzymology; D002850:Chromatography, Gel; D042845:Laccase; D008031:Lignin / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D010088:Oxidoreductases / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D010545:Peroxides; D020106:Acrylamide; D008031:Lignin; D010088:Oxidoreductases; D042845:Laccase