title: A functional cra gene is required for Salmonella enterica serovar typhimurium virulence in BALB/c mice.
abstract: A minitransposon mutant of Salmonella enterica serovar Typhimurium SR-11, SR-11 Fad(-), is unable to utilize gluconeogenic substrates as carbon sources and is avirulent and immunogenic when administered perorally to BALB/c mice (M. J. Utley et al., FEMS Microbiol. Lett., 163:129-134, 1998). Here, evidence is presented that the mutation in SR-11 Fad(-) that renders the strain avirulent is in the cra gene, which encodes the Cra protein, a regulator of central carbon metabolism.
authors.affiliation: Department of Biochemistry, Microbiology, and Molecular Genetics, University of Rhode Island, Kingston, Rhode Island 02881, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Allen
authors.forename: M
authors.initials: M
authors.lastname: Utley
authors.forename: H
authors.initials: H
authors.lastname: van Den Bosch
authors.forename: P
authors.initials: P
authors.lastname: Nuijten
authors.forename: M
authors.initials: M
authors.lastname: Witvliet
authors.forename: B A
authors.initials: BA
authors.lastname: McCormick
authors.forename: K A
authors.initials: KA
authors.lastname: Krogfelt
authors.forename: T R
authors.initials: TR
authors.lastname: Licht
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Mauel
authors.forename: M P
authors.initials: MP
authors.lastname: Leatham
authors.forename: D C
authors.initials: DC
authors.lastname: Laux
authors.forename: P S
authors.initials: PS
authors.lastname: Cohen
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D050260:Carbohydrate Metabolism; D002952:Citric Acid Cycle; D005798:Genes, Bacterial; D007422:Intestines / Q000382:microbiology; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009093:Mucus / Q000382:microbiology; D016254:Mutagenesis, Insertional; D012097:Repressor Proteins / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D012703:Serotyping; D056968:Lac Repressors
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C068837:FruR protein, Bacteria; D056968:Lac Repressors