Hydrolysis of glucosinolates using nylon-immobilized myrosinase to produce pure bioactive molecules.
Bioactive compounds were produced from natural glucosinolates, secondary plant metabolites, using myrosinase (thioglucoside glucohydrolase EC 3.2.3.1) isolated from ripe seeds of Sinapis alba. The enzyme was immobilized on granular nylon 6.6 with the crosslinking technique. Immobilized myrosinase displayed extraordinary operational and storage stability. Using a small thermostatted continuous packed-bed bioreactor, the enzyme activity was unchanged after 15 days of continuous use at 37 degrees C and after >1 year of storage at room temperature. The bioreactor was particularly efficient in producing pure isothiocyanates, but it was less efficient for pure nitrile production.
Leoni
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Istituto Sperimentale per le Colture Industriali of Italian Ministry of Agricultural and Forestry Politics, Via di Corticella, 133, I-40129 Bologna, Italy.
Iori
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Palmieri
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D019149:Bioreactors; D001709:Biotechnology; D004800:Enzymes, Immobilized; D005961:Glucosinolates / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D009149:Mustard Plant / Q000201:enzymology; D009757:Nylons; D010946:Plants, Medicinal
D004800:Enzymes, Immobilized; D005961:Glucosinolates; D009757:Nylons; D006026:Glycoside Hydrolases; C022739:thioglucosidase