Phospholipid fatty acid composition, vitamin E content and susceptibility to lipid peroxidation of duck spermatozoa.
Recent studies on chicken semen have suggested that the lipid and fatty acid composition of spermatozoa may be important determinants of fertility. Phospholipid fatty acid composition, vitamin E content and in vitro susceptibility to lipid peroxidation of duck spermatozoa were investigated using GC-MS and HPLC based methods. The total phospholipid fraction of duck spermatozoa was characterized by high proportions of the n-6 polyunsaturated fatty acids arachidonic (20:4n-6), docosatetraenoic (22:4n-6) and docosapentaenoic (22:5n-6) acids but a substantial proportion of the n-3 fatty acid docosahexaenoic (22:6n-3) acid was also present. Palmitic (16:0) and stearic (18:0) fatty acids were the major saturates in sperm phospholipids. Among the phospholipid classes, phosphatidylserine (PS) had the highest degree of unsaturation due to very high proportions of 22:6n-3, 22:5n-6, 22:4n-6 and 20:4n-6, comprising together more than 75% of total fatty acids in this fraction. Phosphatidylethanolamine (PE) also contained high proportions of these four C(20-22) polyunsaturates, which together formed 60% of total fatty acids in this phospholipid. Spermatozoa and seminal plasma of duck semen were characterized by unexpectedly low content of vitamin E, being more than 4-fold lower than in chicken semen. In duck semen the major proportion of the vitamin E (>70%) was located in the spermatozoa. The very high proportion of 22:6n-3 in PS and PE fractions of duck sperm lipids and the comparatively low levels of vitamin E could predispose semen to lipid peroxidation. Nevertheless the in vitro susceptibilities to Fe2+-stimulated lipid peroxidation of duck and chicken spermatozoa were very similar. The results of the study suggest that increased superoxide dismutase and glutathione peroxidase activity and increased antioxidant activity of seminal plasma may compensate for the low levels of vitamin E to help protect the membranes of duck spermatozoa, which exhibit a high degree of unsaturation from oxidative stress.
Surai
P F
PF
Department of Biochemistry and Nutrition, Scottish Agricultural College Auchincruive, Ayr, Scotland UK. P.Surai@au.sac.ac.uk
Brillard
J P
JP
Speake
B K
BK
Blesbois
E
E
Seigneurin
F
F
Sparks
N H
NH
D000818:Animals; D002645:Chickens / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004372:Ducks / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis*; D015227:Lipid Peroxidation; D008297:Male; D010743:Phospholipids / Q000032:analysis*; D013094:Spermatozoa / Q000737:chemistry* / Q000378:metabolism; D014810:Vitamin E / Q000032:analysis*
D005227:Fatty Acids; D010743:Phospholipids; D014810:Vitamin E