Immunochemical characterization of monoclonal protein in the serum of a patient with Waldenstr]om's macroglobulinemia showing both pyroglobulin and cryoglobulin properties.
A patient with Waldenstr]om's macroglobulinemia whose serum demonstrated properties of both pyroglobulin and cryoglobulin was studied. A monoclonal (M) protein in the serum of the patient was identified by immunoelectrophoresis (IEP) and immunofixation electrophoresis (IFE) as IgM-lambda. The M-protein was separated by gel permeation high-performance liquid chromatography (HPLC). Cryoglobulin in the serum was isolated by the method of cold precipitation. The cryoglobulin was identified as IgM-lambda type by IEP and was the same M-protein as that which occurred in this patient's serum. The purified cryoglobulin also had the properties of a pyroglobulin. Neither property disappeared following pretreatment with 2-mercaptoethanol (2-ME), urea, and Triton X-100 detergent, and deglycosylation with N-glycanase (E.C. 3.5.1.52). We suggest that these abnormal properties were caused by the molecular abnormality of the IgM-lambda M-protein.
Moriyama
T
T
Department of Laboratory Technology, College of Medical Technology, Hokkaido University, N-12, W-5, Kita-Ku, Sapporo, Japan. moriyama@cme.hokudai.a.jp
Torimoto
Y
Y
Kohgo
Y
Y
Ikeda
H
H
Matsuno
K
K
D000368:Aged; D000581:Amidohydrolases / Q000494:pharmacology; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D003450:Cryoglobulins / Q000032:analysis*; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M / Q000097:blood; D007146:Immunoglobulin lambda-Chains / Q000097:blood; D007150:Immunohistochemistry; D008623:Mercaptoethanol / Q000494:pharmacology; D017830:Octoxynol / Q000494:pharmacology; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D011750:Pyroglobulins / Q000032:analysis*; D008258:Waldenstrom Macroglobulinemia / Q000097:blood*
D003450:Cryoglobulins; D007075:Immunoglobulin M; D007146:Immunoglobulin lambda-Chains; D011750:Pyroglobulins; D008623:Mercaptoethanol; D017830:Octoxynol; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase