Changes in hepatic venous oxygen saturation in hepatic warm ischemia/reperfusion injury in pigs.
To clarify the changes that occur in hepatic venous oxygen saturation (ShVO2) during hepatic ischemia/reperfusion (I/R) injury, we examined the relationship between ShVO2, hepatic tissue blood flow (HTBF), and portal vein pressure (PVP) in a warm I/R model using pig livers. Female pigs weighing 18-23 kg were subjected to warm I/R under extracorporeal circulation between the superior mesentric vein and the left jugular vein to avoid portal congestion. The warm ischemic times were 120 min (n = 4), 180 min (n = 14), and 240 min (n = 4). ShVO2, HTBF, and PVP were measured after reperfusion. The survival rates of the pigs 3 days after reperfusion were 100% in the 120-min group, 57% in the 180-min group, and 25% in the 240-min group. In the 180-min group, the ShVO2 was lower in the pigs that died than in those that survived. There was a significant correlation between ShVO2 and both PVP and HTBF after reperfusion. Histological examination revealed findings of severe I/R injury in pigs with a low ShVO2, and mild I/R injury in pigs with a stable ShVO2. These observations suggest that the changes in ShVO2 could reflect the degree of hepatic I/R injury, especially that related to microcirculatory disturbances occurring at the sinusoid levels.
Katsuramaki
T
T
Department of Surgery, Sapporo Medical University, School of Medicine, Japan.
Kimura
H
H
Isobe
M
M
Nagayama
M
M
Meguro
M
M
Nui
A
A
Matsuno
T
T
Ura
H
H
Mukaiya
M
M
Hirata
K
K
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001794:Blood Pressure; D005260:Female; D008099:Liver / Q000098:blood supply* / Q000473:pathology; D008833:Microcirculation; D010100:Oxygen / Q000097:blood*; D011169:Portal Vein / Q000502:physiology; D012039:Regional Blood Flow; D015427:Reperfusion Injury / Q000097:blood* / Q000473:pathology; D013552:Swine
D001219:Aspartate Aminotransferases; D010100:Oxygen