title: A model for sequestration of the transmission stages of Plasmodium falciparum: adhesion of gametocyte-infected erythrocytes to human bone marrow cells.
abstract: With the aim of developing an appropriate in vitro model of the sequestration of developing Plasmodium falciparum sexual-stage parasites, we have investigated the cytoadherence of gametocytes to human bone marrow cells of stromal and endothelial origin. Developing stage III and IV gametocytes, but not mature stage V gametocytes, adhere to bone marrow cells in significantly higher densities than do asexual-stage parasites, although these adhesion densities are severalfold lower than those encountered in classical CD36-dependent assays of P. falciparum cytoadherence. This implies that developing gametocytes undergo a transition from high-avidity, CD36-mediated adhesion during stages I and II to a lower-avidity adhesion during stages III and IV. We show that this adhesion is CD36 independent, fixation sensitive, stimulated by tumor necrosis factor alpha, and dependent on divalent cations and serum components. These data suggest that gametocytes and asexual parasites utilize distinct sets of receptors for adhesion during development in their respective sequestered niches. To identify receptors for gametocyte-specific adhesion of infected erythrocytes to bone marrow cells, we tested a large panel of antibodies for the ability to inhibit cytoadherence. Our results implicate ICAM-1, CD49c, CD166, and CD164 as candidate bone marrow cell receptors for gametocyte adhesion.
authors.affiliation: Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom.
authors.forename: N J
authors.initials: NJ
authors.lastname: Rogers
authors.forename: B S
authors.initials: BS
authors.lastname: Hall
authors.forename: J
authors.initials: J
authors.lastname: Obiero
authors.forename: G A
authors.initials: GA
authors.lastname: Targett
authors.forename: C J
authors.initials: CJ
authors.lastname: Sutherland
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000469:parasitology*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000469:parasitology; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D010963:Plasmodium falciparum / Q000254:growth & development*; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D012098:Reproduction; D017154:Stromal Cells / Q000469:parasitology
chemical_list: D011956:Receptors, Cell Surface