title: Structure and serologic properties of O-specific polysaccharide from Citrobacter freundii possessing cross-reactivity with Escherichia coli O157:H7.
abstract: Citrobacter freundii OCU158 is a serologically cross-reactive strain with Escherichia coli O157:H7. To explore the close relationship between two strains, we have analyzed the chemical structures of O-specific polysaccharides and antigenic properties of lipopolysaccharides (LPSs) of both strains. The structure of O-specific polysaccharides from both strains was found to be identical by chemical and nuclear magnetic resonance analyses, in which D-PerNAc was 4-acetamido-4,6-dideoxy-D-mannose: [-->4)-beta-D-Glc-(1-->3)-alpha-D-PerNAc-(1-->4)-alpha-D-GalNAc-(1 --> 3)-alpha-L-Fuc-(1-->](n). The enzyme immunoassay using LPS derived either from E. coli O157 or from C. freundii could equally detect high levels of serum antibodies against LPS in patients with enterohemorrhagic E. coli (EHEC) O157 infection. Absorption of antibodies in EHEC patient serum by LPS from E. coli O157 or C. freundii, however, showed a difference in the epitopes. This difference was attributable to the epitope specificity of the core region and/or lipid A structure in LPS.
authors.affiliation: Department of Bacteriology, Osaka City University Medical School, Osaka, Japan. nishiuchi@med.osaka-cu.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Nishiuchi
authors.forename: M
authors.initials: M
authors.lastname: Doe
authors.forename: H
authors.initials: H
authors.lastname: Hotta
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood; D002648:Child; D002675:Child, Preschool; D016971:Citrobacter freundii / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D003967:Diarrhea / Q000382:microbiology; D000939:Epitopes / Q000276:immunology; D019453:Escherichia coli O157 / Q000276:immunology*; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology; D006801:Humans; D007106:Immune Sera / Q000097:blood; D007124:Immunoenzyme Techniques; D007223:Infant; D008070:Lipopolysaccharides / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D019081:O Antigens / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000939:Epitopes; D007106:Immune Sera; D008070:Lipopolysaccharides; D019081:O Antigens