Transport of nucleosides in Bacillus subtilis: the effect of purine nucleosides on the cytidine-uptake.
The structural effects of chemical modifications upon the affinity of purine nucleosides to cytidine-transport system in Bacillus subtilis were investigated using a series of modified derivatives. The interaction involves protein molecule(s) which require the presence and proper orientation of the sugar residue and its hydroxylic functions. Moreover, a specific interaction with the heterocyclic ring system is involved in the process which results in a requirement for an aromatic pi -electron system and an absence of a polarizable function at position 6 of the purine heterocycle. The region in the protein responsible for the latter interaction is rather limited and, consequently, a proper nucleoside conformation is required.
Fucik
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Kloudová
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Holý
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D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D003562:Cytidine / Q000378:metabolism*; D007700:Kinetics; D009705:Nucleosides / Q000378:metabolism; D011684:Purine Nucleosides / Q000737:chemistry / Q000494:pharmacology
D009705:Nucleosides; D011684:Purine Nucleosides; D003562:Cytidine