Evidence of hepatic endogenous hydrogen peroxide in bile of selenium-deficient rats.
Hepatic endogenous hydrogen peroxide (H(2)O(2)) in bile of selenium-deficient rats (SeD) was for the first time found using the electron spin resonance (ESR) spin-trap technique, and the relationship between glutathione peroxidase (GPX) activity and H(2)O(2) amount is discussed. Normal rats and four groups of rats fed a selenium-deficient diet with different feeding periods were examined. The results showed that the GPX activity decreased depending on the feeding period with the selenium-deficient diet and that the hepatic endogenous H(2)O(2) amount in the bile of the rats fed the selenium-deficient diet for the longest period (a week before birth to 8 weeks old) was drastically higher than those in other groups of rats (P < 0.005). We found that generation of H(2)O(2) due to the decrease in the GPX activity has a threshold value. The results suggest that an exposure to selenium deficiency for long term will cause oxidative stress.
Ueda
Y
Y
Department of Physical Chemistry, Showa Pharmaceutical University, 3-3165, Higashi-Tamagawagakuen, Machida, Tokyo, 194-8543, Japan.
Matsumoto
K
K
Endo
K
K
D000818:Animals; D001646:Bile / Q000737:chemistry*; D003497:Cyclic N-Oxides / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000032:analysis; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000172:deficiency*
D003497:Cyclic N-Oxides; D017665:Hydroxyl Radical; C069977:2,2-dimethyl-5-hydroxy-1-pyrrolidinyloxy; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D012643:Selenium