Systemic immune deviation in the brain that does not depend on the integrity of the blood-brain barrier.
OVA injected into the brain of normal mice evoked a deviant immune response (brain-associated immune deviation (BRAID)) that was deficient in OVA-specific delayed-type hypersensitivity. This response was not dependent on an intact blood-brain barrier since BRAID was induced even when OVA was injected into a newly created lesion site with extensive BBB leakage. However, newly activated microglia at the injection site 2 days after ablation of the striatum correlated with the loss of BRAID. At day 4 after trauma, when activated microglia were only visible further away from the injection site, BRAID was again able to be induced. In contrast to immune deviation elicited via the eye, an intact spleen was not required for BRAID, nor was BRAID adoptively transferable with spleen cells. In contrast i.v. injection of cervical lymph node cells harvested 8 days after OVA injection into the striatum was able to transfer BRAID into naive animals. Together, these data indicate that immune privilege in the brain is actively maintained and is mediated by an immune deviation mechanism that differs from eye-derived immune deviation and arises even when the BBB is compromised.
Wenkel
H
H
Department of Ophthalmology, Schepens Eye Research Institute, Harvard Medical School, Boston, MA 02114, USA.
Streilein
J W
JW
Young
M J
MJ
D019264:Adoptive Transfer; D000818:Animals; D001812:Blood-Brain Barrier / Q000276:immunology*; D001921:Brain / Q000276:immunology* / Q000378:metabolism; D003342:Corpus Striatum / Q000276:immunology / Q000378:metabolism; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007276:Injections, Intraventricular; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D009333:Neck; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D013154:Spleen / Q000276:immunology
D010047:Ovalbumin