Expression of functional granulocyte colony-stimulating factor receptors on human B-lymphocytic leukemia cells.
We analyzed the expression of cell surface antigens and granulocyte colony-stimulating factor (G-CSF) receptors using flow cytometry, the expression of G-CSF mRNA receptor, using reverse transcription (RT)-PCR, and tested the effect of G-CSF on leukemia colony formation. A total of 14 lymphocytic leukemia patients were examined, seven with acute lymphocytic leukemia (ALL), two with adult T-cell leukemia (ATL), two with B-chronic lymphocytic leukemia (CLL), two with chronic myelocytic leukemia in lymphoid blastic crisis (CML-LBC), and one with plasma cell leukemia (PCL). The presence of G-CSF receptors was demonstrated in 4/14 (29%) patients, two with ALL, one with CLL, and one with CML-LBC, and was associated with stimulation of leukemia clonogenic cell growth by G-CSF. In addition, all four positive leukemia cell types expressed typical B-cell antigens. Our results indicated that G-CSF receptors are expressed on some portion of B-lymphoid leukemia and that their receptors are functional as growth stimulators.
Handa
A
A
First Department of Internal Medicine, Saitama Medical School, Japan. handaa@nih.gov
Kashimura
T
T
Takeuchi
S
S
Yamamoto
A
A
Murohashi
I
I
Bessho
M
M
Hirashima
K
K
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D019921:Biotinylation; D001752:Blast Crisis / Q000097:blood; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D015448:Leukemia, B-Cell / Q000097:blood / Q000235:genetics / Q000378:metabolism; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood; D007951:Leukemia, Myeloid / Q000473:pathology; D007952:Leukemia, Plasma Cell / Q000097:blood; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood; D012333:RNA, Messenger / Q000378:metabolism; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor