Effects of intermittent exposures of aflatoxin B(1) on hepatic and testicular glutathione S-transferase in rats.
Glutathione S-transferase (GST) plays a major role in the detoxification of the potent hepatocarcinogen aflatoxin B(1) (AFB(1)). This study evaluated the effects of intermittent exposures to AFB(1) on hepatic and testicular GST in rats. Male Fischer 344 rats were fed diets containing AFB(1) (0, 0.01, 0.04, 0.4 and 1.6 ppm) intermittently at 4-week intervals up to 20 weeks. The control animals were fed an AFB(1)-free NIH-31 diet. Rats consuming diets with 0.01 ppm AFB(1) did not show the induction of hepatic or testicular GST activity. Intermittent exposures to AFB(1) at concentrations of 0.04-1.6 ppm significantly increased the GST activities. The increase of the enzyme activity was proportional to the dose and length of AFB(1) exposure.
Sahu
S C
SC
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, MD 20708, USA.
Chou
M W
MW
Sotomayor
R E
RE
Hinton
D M
DM
Barton
C N
CN
O'Donnell
M W
MW
D016604:Aflatoxin B1 / Q000096:biosynthesis / Q000633:toxicity*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens / Q000633:toxicity*; D018736:DNA Adducts / Q000096:biosynthesis; D004032:Diet; D005982:Glutathione Transferase / Q000378:metabolism*; D008658:Inactivation, Metabolic; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D013737:Testis / Q000187:drug effects* / Q000201:enzymology
D002273:Carcinogens; D018736:DNA Adducts; C072611:aflatoxin B1-DNA adduct; D016604:Aflatoxin B1; D005982:Glutathione Transferase