title: A modification of Mayer's tannic acid-ferric chloride staining method for demonstrating cellular membranous systems for light microscopy.
abstract: To observe cellular membranous systems under a light microscope, we modified Mayer's tannic acid-ferric chloride stain method by adding a treatment with hematoxylin after the original procedure. We used the modified tannic acid-ferric chloride (MTA-Fe) stain method to examine kidneys, liver, heart, trachea, epididymides and other organs of rats and dogs. The MTA-Fe stain clearly demonstrated the basement membrane, brush border, basolateral invaginations and cell processes in the kidneys which enabled easy differentiation of the S1 and S3 segments of proximal convoluted tubules. Our technique also demonstrated hepatic cell membranes and bile canaliculi in the liver, cross striations and longitudinal traveling of myofibrils in the heart, cilia of the epithelial cells in the trachea, and stereocilia and terminal bars in the epididymis. The MTA-Fe stain is a convenient method to visualize cellular membranous systems even for light microscopy. The stain has the advantages of using no toxic materials, simple and easy technique, little variation of staining results, and little fading for several months after staining.
authors.affiliation: Developmental Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Matsui
authors.forename: K
authors.initials: K
authors.lastname: Hatanaka
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Asano
authors.forename: H
authors.initials: H
authors.lastname: Yamagata
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D002712:Chlorides; D004285:Dogs; D004822:Epididymis / Q000648:ultrastructure; D005260:Female; D005290:Ferric Compounds; D047348:Hydrolyzable Tannins; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008297:Male; D009206:Myocardium / Q000648:ultrastructure; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D014132:Trachea / Q000648:ultrastructure
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D047348:Hydrolyzable Tannins; C024555:ferric chloride