[The role of neutral sphingomyelinase in the interleukin-1beta signal transduction in cells of the mouse cerebral cortex].
Involvement of the sphingomyelin cascade in Interleukin 1 beta (IL-1) signal transduction pathway in membrane fraction P2 of the murine brain cortex, was found. A key role of the membrane enzyme neutral sphingomyelinase (nSMase) in triggering the sphingomyelin pathway for IL-1 beta, was confirmed. The IL-1 beta was shown to activate in a dose-dependent manner nSMase in the P2 fraction of the brain cortex. Employment of both brain cortex membranes from the mice deficient in the type I IL-1 receptor and of IL-1 receptor antagonist made it possible to obtain evidence on the necessity of the IL-1 beta binding to the type I IL-1 receptor for the nSMase activation. It appears that the IL-1 beta effects on the CNS are realized via IL-1 receptor type I and activation of the nSMase as an initiating enzyme of the sphingomyelin cascade.
Rybakina
E G
EG
Institute of Experimental Medicine of the Russian Acad. Med. Sci., St. Petersburg, Russia.
Nalivaeva
N N
NN
Pivanovich
I Iu
IIu
Shanin
S N
SN
Kozinets
I A
IA
Korneva
E A
EA
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D008213:Lymphocyte Activation; D008297:Male; D008566:Membranes; D051379:Mice; D018345:Mice, Knockout; D018123:Receptors, Interleukin / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology*; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism*
D007375:Interleukin-1; D018123:Receptors, Interleukin; D013108:Sphingomyelin Phosphodiesterase