Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis.
The high resolving power of two-dimensional polyacrylamide gel electrophoresis 2D-PAGE and its full analytical and preparative potential have been described with special emphasis on reproducibility and standardization of protein spot patterns, enhanced protein detection sensitivity, and computer analysis database development. New methodologies for peptide mass fingerprinting, peptide, sequence, and fragmentation tagging have been highlighted. Major challenges associated with 2D-PAGE/mass spectrometric protein sequencing were outlined which need to be addressed in the future, including sample enrichment, use of alternative gel matrices, improvements in separation systems interfaced directly to the mass spectrometer, and design of high-sensitivity instruments with very high mass ranges. It is hoped that comparative studies to identify, quantitate, and characterize proteins differentially expressed in normal versus diseased cells would give insight into mechanisms of pathogenesis and allow the development of a way to control both the etiology and the course of diseases.
Appella
E
E
Laboratory of Cell Biology, National Institutes of Health, Bethesda, MD 20892, USA.
Arnott
D
D
Sakaguchi
K
K
Wirth
P J
PJ
D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D020539:Sequence Analysis, Protein
D011506:Proteins; D020543:Proteome