Common antigenicity between Japanese cedar (Cryptomeria japonica) pollen and Japanese cypress (Chamaecyparis obtusa) pollen, I. H-2 complex affects cross responsiveness to Cry j 1 and Cha o 1 at the T- and B-cell level in mice.
Common antigenicity among two purified Japanese cedar pollen allergens (Cry j 1 and Cry j 2) and one Japanese cypress pollen allergen (Cha o 1) was explored at the T-cell and B-cell level in mice of different H-2 haplotypes. Cry j 2 did not show any common antigenicity with Cry j 1 or Cha o 1. B10.S (H-2S) mice immunized with Cry j 1 or Cha o 1 generated T cells and antibodies reactive to both antigens, indicating the common antigenicity of these antigens. C57BL/6 (H-2b) mice were non-responders to Cry j 1. BALB/c (H-2d) mice immunized with Cry j 1 or Cha o 1 and C57BL/6 mice immunized with Cha o 1 generated T cells that were only reactive with the respective immunogen, but produced antibody reactive to both Cry j 1 and Cha o 1, indicating that Cry j 1 and Cha o 1 share their B-cell epitope but not their T-cell epitope. This finding may provide a clue for the clarification of the T-cell and B-cell epitopes of Cry j 1 and Cha o 1, even though the data are influenced by H-2 complex restriction in mice. Considering that H-2 complex restriction affects cross responsiveness to Cry j 1 and Cha o 1 at the T- and B-cell level in mice, we assessed the possible situation in humans exposed sequentially to Japanese cedar pollen and Japanese cypress pollen.
Kingetsu
I
I
Department of Internal Medicine (III), Institute of DNA Medicine, The Jikei University School of Medicine, Tokyo, Japan.
Ohno
N
N
Hayashi
N
N
Sakaguchi
M
M
Inouye
S
S
Saito
S
S
D000485:Allergens / Q000276:immunology*; D000818:Animals; D052179:Antigens, Plant; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D002455:Cell Division / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014197:Trees
D000485:Allergens; D052179:Antigens, Plant; C408445:Cha o 1 protein, Chamaecyparis obtusa; C065290:Cry j I protein, Cryptomeria japonica; D000939:Epitopes; D006183:H-2 Antigens; D007074:Immunoglobulin G; D010940:Plant Proteins