Molecular characterization of a hydroxymethylglutaryl-CoA reductase gene from mulberry (Morus alba L.).
The genus Morus consists of trees and shrubs, which are distributed in temperate and subtropical regions. Commonly known as mulberry, a few of the Morus species are valued for their foliage, which constitutes the chief feed for mulberry silkworms. Steroids and isoprenoid compounds present in the foliage not only add nutritive factors to the feed but also contribute greatly to silkworm health and silk production. Mevalonate synthesis, which is the first step in isoprenoid biosynthesis, is catalyzed by the enzyme hydroxymethylglutaryl-CoAreductase (HMGR). A genomic clone, Mahmg1, was isolated from Morus alba and its expression characterized in mulberry and transgenic tobacco. In mulberry, Mahmg1 transcripts were highest in young leaves and flowers. The promoter region of the Mahmg1 gene was fused to the beta-glucuronidase (GUS) reporter gene and the fusion introduced into tobacco. In imbibed embryos, GUS expression was limited to the cotyledons, epicotyl, and root elongation zone. Later, GUS staining was observed in floral tissues, guard cells, and the heads of trichomes on the stem and petioles. Mahmg1::GUS activity increased 3-4-fold by treatment with 100 microM abscisic acid and 15-80-fold in dark-grown versus light-grown seedlings. These results show that expression of the Mahmg1 gene is differentially regulated by developmental and environmental cues, suggesting that its HMGR isozyme a may provide a precursor for synthesis of specific isoprenoids during mulberry growth and development.
Jain
A K
AK
Department of Biology, Texas A&M University, College Station 77843-3258, USA.
Vincent
R M
RM
Nessler
C L
CL
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D003624:Darkness; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D008027:Light; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013314:Stress, Mechanical; D014026:Nicotiana / Q000235:genetics; D014197:Trees / Q000201:enzymology / Q000235:genetics*
D018744:DNA, Plant; D010937:Plant Growth Regulators; D011993:Recombinant Fusion Proteins; D006903:Hydroxymethylglutaryl CoA Reductases; D005966:Glucuronidase