title: Immunohistochemical staining of human alpha-defensin-1 (HNP-1), in the submandibular glands of patients with oral carcinomas.
abstract: The purpose of this study was the immunohistochemical localization and distribution of HNP-1 in the submandibular glands of patients with oral carcinomas. Tissue sections were embedded in paraffin, and HNP-1 was immunostained by the streptavidin-biotin coupled peroxidase method. Striated duct cells in the submandibular glands were stained with anti-defensin antibody. Neutrophils and capillary intimal cells were also stained. Defensins (HNPs) are peptides that occur in neutrophils and protect against bacteria and tumor cells. Human alpha-defensin-1 (HNP-1) is such a peptide, possessing both antimicrobial and cytotoxic activities. The presence of HNP-1 in striated duct cells in the submandibular glands of oral cancer patients, suggests a likely role in tumor immunity, for this peptide.
authors.affiliation: Department of Oral and Maxillofacial Surgery 1, Okayama University Dental School, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Mizukawa
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
authors.forename: M
authors.initials: M
authors.lastname: Kamio
authors.forename: E
authors.initials: E
authors.lastname: Yamachika
authors.forename: T
authors.initials: T
authors.lastname: Ueno
authors.forename: J
authors.initials: J
authors.lastname: Fukunaga
authors.forename: S
authors.initials: S
authors.lastname: Takagi
authors.forename: T
authors.initials: T
authors.lastname: Sugahara
mesh_terms: D002196:Capillaries / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology* / Q000601:surgery*; D023082:Defensins; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000473:pathology* / Q000601:surgery*; D009504:Neutrophils / Q000473:pathology; D011506:Proteins / Q000032:analysis*; D013363:Submandibular Gland / Q000098:blood supply / Q000473:pathology*; D023084:alpha-Defensins
chemical_list: D023082:Defensins; D011506:Proteins; D023084:alpha-Defensins; C406413:human neutrophil peptide 1