A novel bacteriorhodopsin-like protein from Haloarcula japonica strain TR-1: gene cloning, sequencing, and transcript analysis.
The gene encoding a novel bacteriorhodopsin (bR)-like protein from Haloarcula japonica strain TR-1 was cloned and sequenced. The nucleotide sequence of the gene contained an open reading frame that corresponded to a protein of 250 amino acids. The deduced amino acid sequence of Ha. japonica bR-like protein exhibited the highest homology to those of cruxrhodopsins (cRs) produced by members of the genus Haloarcula, suggesting that the bR-like protein of Ha. japonica belonged to the cR subfamily. The hydropathy analysis of Ha. japonica bR-like protein (cR) revealed that the Ha. japonica cR had a transmembrane heptahelical structure similar to that of bR. Furthermore, transcription of the cR gene in Ha. japonica was confirmed by the reverse transcription-polymerase chain reaction method.
Yatsunami
R
R
School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Kawakami
T
T
Ohtani
H
H
Nakamura
S
S
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics*; D001436:Bacteriorhodopsins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D019613:Haloarcula / Q000235:genetics*; D008969:Molecular Sequence Data; D017494:Proton Pumps / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D019843:Archaeal Proteins; D017494:Proton Pumps; D012333:RNA, Messenger; C410125:bacteriorhodopsin-like protein, Haloarcula japonica; C091296:cop-2 protein, Haloarcula japonica; C091297:cruxrhodopsin-1 protein, Haloarcula japonica; D001436:Bacteriorhodopsins