IL-12 induces SDS-stable class II alphabeta dimers in murine dendritic cells.
We assessed the effect of rIL-12 on the expression of class II molecules and on the ratio between SDS-stable and unstable alphabeta dimers in dendritic cells. We found that in vitro exposure of the cells to IL-12 increased their surface expression of mature class II molecules, despite a marked decline in class II biosynthesis. This effect was accompanied by a striking increase in the overall proportion of SDS-stable heterodimers.
Grohmann
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Department of Experimental Medicine, University of Perugia, Italy. grohmann@tin.it
Orabona
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Bianchi
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Belladonna
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Fioretti
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Puccetti
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D000818:Animals; D015153:Blotting, Western / Q000379:methods; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D019281:Dimerization; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D008811:Mice, Inbred DBA; D012967:Sodium Dodecyl Sulfate
D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; D018664:Interleukin-12; D012967:Sodium Dodecyl Sulfate