title: The mapping and ordering of fragments of SV40 DNA produced by restriction endonucleases.
abstract: SV40 DNA is cleaved by the Eco RII and Hae restriction endonucleases to give rise to two different sets of 16 fragments each. These fragments have been ordered by analysis of the products of redigestion of one set of fragments with another restriction enzyme. The cleavage sites have been precisely mapped based on length measurements of the products of each cleavage. This provides a convenient group of small DNA fragments suitable for sequence analysis investigation of the transcripts present in infected cells, or construction of deletion substitution variants of the virus.
authors.forename: K N
authors.initials: KN
authors.lastname: Subramanian
authors.forename: J
authors.initials: J
authors.lastname: Pan
authors.forename: S
authors.initials: S
authors.lastname: Zain
authors.forename: S M
authors.initials: SM
authors.lastname: Weissman
mesh_terms: D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000737:chemistry* / Q000235:genetics
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes