Effect of apolipoprotein E on cell viability in a human neuroblastoma cell line: influence of oxidation and lipid-association.
Apolipoprotein E (apoE) whose polymorphic expression is widely associated with Alzheimer's disease (AD) is one of the most studied protein present in cerebral amyloid deposits. Native or fragments of apoE are known to exert neurotoxic effects. We evaluated the effects of apoE oxidation and lipid-association on the viability of human neuroblastoma IMR32 cells. We show that apoE affects cell viability only when it is lipid-associated and applied at a concentration near to that found in plasma, and this whatever the isoform. Oxidized phospholipid-associated apoE has a similar impact on cell viability. These findings show the necessity of including apoE into phospholipids when studying its effect on cell metabolism and underline the probable intervention of surface heparan sulfate proteoglycans (HSPG). It also warrants further studies in order to delineate the pathophysiological importance of apoE.
Hoy
A
A
Centre du Médicament, Université Henri Poincaré Nancy I, 30 rue Lionnois, 54000, Nancy, France.
Leininger-Muller
B
B
Jolivalt
C
C
Siest
G
G
D053329:Apolipoprotein E2; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D009447:Neuroblastoma; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000494:pharmacology*; D020033:Protein Isoforms / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D053329:Apolipoprotein E2; D053318:Apolipoprotein E3; D053327:Apolipoprotein E4; D001057:Apolipoproteins E; D010743:Phospholipids; D020033:Protein Isoforms