title: Short-term changes of NADPH diaphorase-exhibiting neuronal pools in the spinal cord of rabbit after repeated sublethal ischemia.
abstract: The aim of this study was the histochemical characterization of NADPH diaphorase-positive neuronal pools in the rabbit lumbosacral segments using a model of single, repeated and multiple sublethal spinal cord ischemia. Following a single 8-min sublethal spinal cord ischemia and 1-hour reperfusion, the staining of NADPH diaphorase-exhibiting neurons in the dorsal horn, pericentral region, dorsal gray commissure and sacral parasympathetic nucleus was comparable with the control sections. In contrast to the foregoing sublethal ischemia, a regionally different somatic NADPH diaphorase (NADPHd) staining was found after multiple sublethal spinal cord ischemia. Whereas an almost complete loss of the staining of large NADPHd-exhibiting somata in the pericentral region was detected, the staining of the NADPHd-exhibiting neuronal pools in the deep dorsal horn and sacral parasympathetic nucleus was fully preserved. Concomitantly, a prominent reduction of small NADPH diaphorase-positive neurons was noted in the superficial dorsal horn layers of lower lumbar and sacral segments.
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Kosice, Slovak Republic. marsala@saske.sk
authors.forename: J
authors.initials: J
authors.lastname: Marsala
authors.forename: P
authors.initials: P
authors.lastname: Jalc
mesh_terms: D000818:Animals; D005260:Female; D006651:Histocytochemistry; D007511:Ischemia / Q000201:enzymology*; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009474:Neurons / Q000201:enzymology*; D011817:Rabbits; D013116:Spinal Cord / Q000098:blood supply* / Q000201:enzymology*; D014018:Tissue Distribution
chemical_list: D009252:NADPH Dehydrogenase