Assays for functional binding of plasminogen to viral proteins.
Cleavage of the hemagglutinin (HA) molecule by proteases is a prerequisite for the infectivity of influenza A viruses. Plasminogen binds to the viral glycoprotein neuraminidase (NA), and NA-bound plasminogen is activated to plasmin, which cleaves the HA of influenza A/WSN/33 (WSN) (H1N1) virus. Here we present assays for detecting functional plasminogen binding to the influenza virus NA.
Goto
H
H
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive West, Madison, Wisconsin 53706, USA.
Kawaoka
Y
Y
D000818:Animals; D002460:Cell Line; D004285:Dogs; D005341:Fibrinolysin / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D009439:Neuraminidase / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; D010958:Plasminogen; D009439:Neuraminidase; D005341:Fibrinolysin