Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria.
Arbitrarily primed (AP)-PCR can be used to generate characteristic DNA fingerprint patterns. However, small changes in reaction conditions can cause band irreproducibility. In this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. A single triplicate AP-PCR (TAP-PCR) procedure, using an 18-mer primer, was developed and used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.
Cusick
S M
SM
Department of Food Science and Nutrition and Department of Microbial Engineering, University of Minnesota, St. Paul, Minnesota 55108, USA.
O'Sullivan
D J
DJ
D001419:Bacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D001644:Bifidobacterium / Q000145:classification; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019344:Lactic Acid / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000145:classification; D007934:Leuconostoc / Q000145:classification; D010376:Pediococcus / Q000145:classification; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid