[Study of interleukin-6 and tumor necrosis factor-alpha levels in maternal serum and amniotic fluid in patients with preterm premature rupture of membrane].
OBJECTIVE
To study the change and clinical significance of maternal serum and amniotic fluid interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) levels in patients with chorioamnionitis and premature rupture of membrane (PROM).


METHODS
Twenty normal term-pregnant women as control, and fourty-six women with PROM were enrolled for study. Maternal serum and amniotic fluid IL-6, TNF-alpha levels were measured by radioimmunoassay and enzyme-labeled immunosorbent assay (ELISA). Chorioamnionitis was diagnosed histologically.


RESULTS
The maternal serum IL-6 levels and amniotic fluid IL-6, TNF-alpha levels were higher in PROM group than that of control (P < 0.01). There was significant positive correlation between maternal IL-6, amniotic fluid IL-6, TNF-alpha levels and the duration from rupture of membrane, (the longer was time, the higher were the maternal IL-6 and the amniotic fluid IL-6. TNF-alpha). There were 12 cases with chorioamnionitis in patients with PROM and their maternal and amnotic fluid IL-6, TNF-alpha levels were higher than that of non-chorioamnionitis (P < 0.01-0.05).


CONCLUSIONS
IL-6 and TNF-alpha levels in maternal serum and amniotic fluid are valuable clinical indices for identification of chorioamnionitis in patients with PROM.
Wang
L
L
208th Hospital of PLA, Changchun.
Zhang
W
W
Wang
Y
Y
D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism*; D002821:Chorioamnionitis / Q000378:metabolism; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism*; D011247:Pregnancy; D011897:Random Allocation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha