Intron-mediated gusA expression in tritordeum and wheat resulting from particle bombardment.
The promoterless maize ubiquitin first exon and intron fragment can drive gusA expression in immature tritordeum inflorescences and immature wheat scutella. In fluorescence assays, this fragment induces gusA expression in tritordeum inflorescences to 50 times higher than background. The activity of the complete promoter, exon and intron cassette was up to 20000-fold higher than background but the maize ubiquitin promoter in isolation had very low activity. A construct with the maize alcohol dehydrogenase first exon and intron had low activity, visible in histochemical assays. Both intron sequences have promoter-like features and in the ubiquitin intron there is a sequence homologous to the opaque-2-binding box. We suggest that the combination of these elements may explain the promoter activity detected in these introns.
Salgueiro
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Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, UK.
Pignocchi
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Parry
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D000426:Alcohol Dehydrogenase / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005821:Genetic Techniques; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D001467:Hordeum / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D018514:Plant Structures / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics*; D003313:Zea mays / Q000201:enzymology
D011993:Recombinant Fusion Proteins; D000426:Alcohol Dehydrogenase; D005966:Glucuronidase