title: Bis[(benzo-15-crown-5)-15-yl methyl] pimelate forms ion channels in planar lipid bilayer: a novel model ion channel.
abstract: Some crown ethers translocate cations across the liposomal membrane either by a carrier mechanism or by forming ion channels. We report formation of ion channels in lipid bilayer membranes by bis[(benzo-15-crown-5)-15-yl methyl] pimelate, a crown ether known to form ion inclusion complexes with alkali metal cations. The channels have characteristic long openings lasting several seconds and a low conductance (4 pS in 500 mM KCl and 2.5 pS in 500 mM NaCl). A model of the crown ether channel formed by stacking of four monomers is proposed. A large database of structural information on crown ethers and their ion inclusion complexes as well as large family of crown ethers with a variety of substitutions in the ring are commercially available. Thus the crown ether channel is an attractive model system to study the role of various chemical moieties in ion conduction which may provide deeper insight into understanding the mechanism(s) of selectivity, ion transport, etc. in biological ion channels.
authors.affiliation: Department of Biophysics, University of Delhi South Campus, New Delhi, India.
authors.forename: V
authors.initials: V
authors.lastname: Vijayvergiya
authors.forename: P
authors.initials: P
authors.lastname: Ghosh
authors.forename: A K
authors.initials: AK
authors.lastname: Bera
authors.forename: S
authors.initials: S
authors.lastname: Das
mesh_terms: D002414:Cations, Monovalent; D043844:Crown Ethers; D004988:Ethers, Cyclic / Q000737:chemistry*; D007473:Ion Channels; D008051:Lipid Bilayers / Q000737:chemistry*; D008564:Membrane Potentials; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D010539:Permeability; D010713:Phosphatidylcholines / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry*; D011188:Potassium; D012964:Sodium
chemical_list: D002414:Cations, Monovalent; D043844:Crown Ethers; D004988:Ethers, Cyclic; D007473:Ion Channels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; C409444:bis((benzo-15-crown-5)-15-yl-methyl)pimelate; C021016:1,2-diphytanoylphosphatidylcholine; D012964:Sodium; D011188:Potassium