[Catalytic properties of Glu-plasminogen in a complex with monoclonal antibody IV-1c].
Earlier it was shown that anti-plasminogen monoclonal antibody IV-1c was able to induce a catalytic activity in plasminogen. IV-1c activates plasminogen by binding to plasminogen protease domain with antigen binding site and to lysine-binding sites by C-terminal lysines of gamma-chains. The effect of plasminogen and IV-1c concentration on rate of catalytic activity induce in Pg-IV-1c complex has been investigated. It was found that IV-1c inhibited an activation reaction at concentrations higher of equimolar to Glu-Pg. Glu-Pg did not inhibit reaction of activation in higher to IV-1c concentrations. Role of IV-1c gamma-chain C-terminal lysine concentration in Pg activation is discussed.
Slominskiĭ
A Iu
AIu
O.V. Palladin Institute of Biochemistry Natl. Acad. Sci. of Ukraine.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002384:Catalysis; D004789:Enzyme Activation; D006801:Humans; D010958:Plasminogen / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D010958:Plasminogen