Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells.
The MA-01 antigen, a thermolabile 210 kDa microtubule-interacting protein, is present in P19 embryonal carcinoma cells on microtubular structures as well as in cytosol. After aggregation of the cells and subsequent incubation with all-trans-retinoic acid (RA), the level of MA-01 expression increased approximately 10 times during 15 days. The increase started after 2 days of incubation with RA and preceded the appearance of neuron-specific tubulin betaIII, MAP2C and neurofilaments. Such elevated expression of MA-01 antigen was not detected in P19 cells treated with dimethylsulfoxide. These data indicate that enhanced expression of MA-01 antigen is one of the earliest events occurring in P19 cells during neuronal differentiation.
Zíková
M
M
Institute of Molecular Genetics, Czech Academy of Sciences, Vídenská 1083, 142 20, Praha, Czech Republic.
Sulimenko
V
V
Dráber
P
P
Dráberová
E
E
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D018236:Carcinoma, Embryonal / Q000378:metabolism* / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D016501:Neurites / Q000187:drug effects; D011233:Precipitin Tests; D011489:Protein Denaturation; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000906:Antibodies; D015415:Biomarkers; D008869:Microtubule-Associated Proteins; D014212:Tretinoin; D004121:Dimethyl Sulfoxide