Morphology of perineuronal nets in tenascin-R and parvalbumin single and double knockout mice.
Recently identified chondroitin sulphate proteoglycans in perineuronal nets include neurocan and phosphacan. However, the function and assembly of these components has yet to be resolved. In this study we show morphological alteration in Wisteria floribunda labelled nets around cortical interneurones both in tenascin-R knockout and tenascin-R/parvalbumin double knockout mice. This alteration reflects the loss of phosphacan and neurocan from cortical nets in mice deficient in tenascin-R. No effect on the membrane related cytoskeleton, as revealed by ankyrin(R), was observed in any of the mice. These results on mice lacking tenascin-R substantiate previously reported in vitro interactions between tenascin-R and phosphacan and neurocan.
Haunsø
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Institute of Histology and General Embryology and Program in Neuroscience, University of Fribourg, CH-1705, Fribourg, Switzerland.
Ibrahim
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M
Bartsch
U
U
Letiembre
M
M
Celio
M R
MR
Menoud
P
P
D000818:Animals; D048429:Cell Size / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D037181:Lectins, C-Type; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058580:Neurocan; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010320:Parvalbumins / Q000235:genetics* / Q000378:metabolism*; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5; D019063:Tenascin / Q000235:genetics* / Q000378:metabolism*
D011508:Chondroitin Sulfate Proteoglycans; D037181:Lectins, C-Type; D009419:Nerve Tissue Proteins; D058580:Neurocan; D010320:Parvalbumins; D019063:Tenascin; C074638:tenascin R; C076817:NCAN protein, human; C516895:PTPRZ1 protein, human; C516896:Ptprz1 protein, mouse; D054633:Receptor-Like Protein Tyrosine Phosphatases, Class 5