Pregnancy induced mammary tumor specific effector cells are present long after parturition in a breast cancer model in rats.
Pregnancy is known to provide protection against 7,12 dimethylbenz[a]anthracene-(DMBA) induced mammary carcinogenesis in rats. We observed in earlier studies that splenocytes of parous rats have significant cytotoxicity against mammary tumor cells both in vitro and in vivo. However, it remains to be established how long these cytolytic cells persist after parturition in parous host. The present study was designed using parous rats, 36 or more days after parturition. We observed that fresh splenocytes from these rats had low cytolytic activity against mammary tumor cells. However, when these cells were re-stimulated with irradiated mammary tumor cells in vitro, they had significantly higher cytotoxicity against mammary tumor cells. These studies show for the first time that pregnancy induced cytotoxic splenocytes are present long after parturition and they can be restimulated in vitro to enhance the cytotoxic effect.
Chakravarty
P K
PK
Breast Cancer Research Unit, Roswell Park Cancer Institute, Elm and Carlton St, Buffalo, NY, USA. chakrava@aecom.yu.edu
Sinha
D K
DK
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D005260:Female; D007743:Labor, Obstetric; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*; D010298:Parity; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D011830:Radiation Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D015127:9,10-Dimethyl-1,2-benzanthracene