Simultaneous immunofluorescent detection of coentrapped cells in gel beads.
An immunofluorescent method involving double color labeling and confocal microscopy was reported to specifically detect lactic acid bacteria and probiotic cells coimmobilized in gels beads. The method described is rapid (4 h) and sensitive and may be useful for studying cell dynamics during mixed-culture starter production using immobilized cells in gel beads. Microscopic observations were perfectly correlated to cell counts obtained using a sandwich enzyme-linked immunosorbent assay.
Prioult
G
G
Dairy Research Centre STELA, Pavillon Paul Comtois, Université Laval, Quebec, Canada G1K 7P4.
Lacroix
C
C
Turcotte
C
C
Fliss
I
I
D001644:Bifidobacterium / Q000166:cytology* / Q000302:isolation & purification; D018914:Cells, Immobilized; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D005782:Gels; D017045:Lactococcus / Q000166:cytology* / Q000302:isolation & purification; D013294:Lactococcus lactis / Q000166:cytology / Q000302:isolation & purification; D018613:Microscopy, Confocal / Q000379:methods
D005456:Fluorescent Dyes; D005782:Gels