Influence of synthetic peptide corresponding to the ACTH-like sequence of human immunoglobulin G1 on proliferation of lymphoblastoid cells.
Influence of the ACTH-like peptide H-Val-Lys-Lys-Pro-Gly-Ser-Ser-Val-Lys-Val-OH corresponding to the sequence 11-20 of the variable part of human immunoglobulin G1 (IgG1) heavy chain on growth of MT-4 human T-lymphoblastoid cell line was investigated. It was found that the ACTH-like peptide at concentration range 10(-11) -10(-7) M inhibits the proliferation of MT-4 cells. Labeled ACTH 'address segment' [(125)I]ACTH (13-24) was used to establish that MT-4 cells express specific receptors for ACTH (K(d) = 97 pM). The ACTH-like peptide and human ACTH (but not IgG1 heavy chain) were shown to compete with [(125)I]ACTH (13-24) for binding to these receptors (K(i1) = 0.38 nM and K(i2) = 0.34 nM).
Lepikhova
T N
TN
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142292 Pushchino, Moscow, Russia. tlepik@fibkh.serpukhov.su
Navolotskaya
E V
EV
Zargarova
T A
TA
Nurieva
R I
RI
Lipkin
V M
VM
Zav'yalov
V P
VP
D000324:Adrenocorticotropic Hormone / Q000737:chemistry* / Q000493:pharmacokinetics; D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000494:pharmacology*; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000494:pharmacology; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D018043:Receptors, Corticotropin / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects
C082552:ACTH (13-24); D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D010446:Peptide Fragments; D018043:Receptors, Corticotropin; D000324:Adrenocorticotropic Hormone