Detection of radiation effects using recombinant bioluminescent Escherichia coli strains.
Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::luxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein-damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma-rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.
Min
J
J
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology (K-JIST), Korea.
Lee
C W
CW
Moon
S H
SH
LaRossa
R A
RA
Gu
M B
MB
D001426:Bacterial Proteins / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects*; D029968:Escherichia coli Proteins; D005720:Gamma Rays; D006360:Heat-Shock Proteins / Q000235:genetics; D008163:Luminescent Measurements; D009876:Operon; D010544:Peroxidases / Q000235:genetics; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C068113:GrpE protein, Bacteria; C489400:GrpE protein, E coli; D006360:Heat-Shock Proteins; D010544:Peroxidases; C076136:catalase HPI; D011938:Rec A Recombinases