A molecular analysis of NKT cells: identification of a class-I restricted T cell-associated molecule (CRTAM).
cDNA library subtraction techniques were used to identify transcripts expressed by activated mouse alphabetaTCR+ CD4-CD8- (double-negative; DN) T cells, a subset of natural killer T (NKT) cells. The most frequent cDNAs identified included the chemokines TCA3, macrophage inflammatory protein-1alpha (MIP-1alpha), MIP-1beta, and lymphotactin (LPTN), the cytokines interleukin-4 (IL-4) and interferon-gamma (IFN-gamma), and a granzyme. We also identified a new member of the immunoglobulin superfamily (Ig-SF). This molecule was designated class I-restricted T cell-associated molecule (CRTAM) as a result of its restricted expression pattern in T cells. Human CRTAM was also identified, and shares the same expression pattern as the mouse molecule. LPTN and CRTAM exhibit the same expression pattern in T cells, suggesting the existence of a gene expression program common to class I-MHC-restricted T cells.
Kennedy
J
J
Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304, USA.
Vicari
A P
AP
Saylor
V
V
Zurawski
S M
SM
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Zlotnik
A
A
D000595:Amino Acid Sequence; D000818:Animals; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002478:Cells, Cultured; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000235:genetics; D002874:Chromosome Mapping; D017124:Conserved Sequence; D016207:Cytokines / Q000235:genetics*; D005260:Female; D015723:Gene Library; D005805:Genes, MHC Class I; D008040:Genetic Linkage; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000235:genetics*; D007371:Interferon-gamma / Q000235:genetics; D007378:Interleukins / Q000235:genetics; D007694:Killer Cells, Natural / Q000145:classification / Q000276:immunology*; D008213:Lymphocyte Activation; D019402:Macrophage Inflammatory Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae; D009928:Organ Specificity; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013950:Thymus Gland / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D015704:CD4 Antigens; D016827:CD8 Antigens; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D007136:Immunoglobulins; D007378:Interleukins; D019402:Macrophage Inflammatory Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D014409:Tumor Necrosis Factor-alpha; C408522:class-I restricted T cell-associated molecule; D007371:Interferon-gamma