title: Refined genetic and comparative physical mapping of the canine copper toxicosis locus.
abstract: Recently, the copper toxicosis (CT) locus in Bedlington terriers was assigned to canine chromosome region CFA10q26, which is homologous to human chromosome region HSA2p13-21. A comparative map between CFA10q21-26 and HSA2p13-21 was constructed by using genes already localized to HSA2p13-21. A high-resolution radiation map of CFA10q21-26 was constructed to facilitate positional cloning of the CT gene. For this map, seven Type I and eleven Type II markers were mapped. Using homozygosity mapping, the CT locus could be confined to a 42.3 cR(3000) region, between the FH2523 and C10.602 markers. On the basis of a partial BAC contig, it was estimated that 1-cR(3000) is equivalent to approximately 210 kb, implying that the CT candidate region is therefore estimated to be about 9 Mb.
authors.affiliation: Department of Medical Genetics, KC04.084.2, University Medical Center Utrecht, WKZ, Lundlaan 6, 3584 EA Utrecht, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: van de Sluis
authors.forename: S
authors.initials: S
authors.lastname: Kole
authors.forename: M
authors.initials: M
authors.lastname: van Wolferen
authors.forename: N G
authors.initials: NG
authors.lastname: Holmes
authors.forename: P L
authors.initials: PL
authors.lastname: Pearson
authors.forename: J
authors.initials: J
authors.lastname: Rothuizen
authors.forename: B A
authors.initials: BA
authors.lastname: van Oost
authors.forename: C
authors.initials: C
authors.lastname: Wijmenga
mesh_terms: D000818:Animals; D003300:Copper / Q000633:toxicity*; D004247:DNA / Q000235:genetics; D004283:Dog Diseases / Q000139:chemically induced / Q000235:genetics*; D004285:Dogs; D005260:Female; D006239:Haplotypes; D006720:Homozygote; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D010375:Pedigree; D020161:Physical Chromosome Mapping
chemical_list: D003300:Copper; D004247:DNA