title: Stimulation of fibroblast growth in vitro by intermittent radiant warming.
abstract: A number of clinical studies have suggested that radiant heat improves the healing of selected acute and chronic wounds. The purpose of this study was to investigate in vitro the effect of intermittent radiant heating on the growth of human skin fibroblasts using a radiant heat-producing dressing with a designated temperature of 38 degrees C. In initial experiments cells were seeded in six well-plates, maintained in culture at 33-34 degrees C, and warmed daily for three cycles of 1 hour with 1.5 hour intervals. Changes in cell growth and metabolism were determined in sets of triplicate wells by cell counts and a colorimetric assay before and after one week's treatment. After eight days the number of cells in the radiant heat-treated group was 30% higher and the metabolic activity 47%- 90% higher than in the control group. In quiescent fibroblasts which had been maintained for four weeks in low-serum medium, the warming regime completely prevented the decrease in cell number observed in control cells. Our findings suggest that the stimulation of cell proliferation induced by intermittent heating in vitro may indicate a possible mechanism contributing to in vivo effects.
authors.affiliation: Wound Healing Institutea, Churchill Hospital, Oxford, UK.
authors.forename: Z
authors.initials: Z
authors.lastname: Xia
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: M A
authors.initials: MA
authors.lastname: Hughes
authors.forename: G W
authors.initials: GW
authors.lastname: Cherry
mesh_terms: D000293:Adolescent; D000328:Adult; D001707:Biopsy, Needle; D001831:Body Temperature; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007871:Leg Ulcer / Q000473:pathology* / Q000628:therapy*; D008297:Male; D008991:Monitoring, Physiologic; D011336:Probability; D012016:Reference Values; D014945:Wound Healing / Q000502:physiology