An in vitro model of ischemic-like stress in retinal pigmented epithelium cells: protective effects of antioxidants.
We have developed a model of in vitro cell oxidative stress in bovine retinal pigment epithelium cells exposed to a ischemia-like condition obtained by interference with glucose utilization through both oxidative phosphorylation and glycolysis. This resulted in a statistically significant decrease of the intracellular ATP levels, which reflects a bioenergetic decline similar to that associated with mitochondrial damage or loss in normal post-mitotic cells aging in vivo. This new model of cellular oxygen stress seems adequate for investigation of the protective action of antioxidants, in agreement with our finding of a statistically significant increase in the ATP levels over the values of the non-treated samples in retinal pigment epithelium cells exposed to the above oxygen stress in medium supplemented with 300 microM vitamin C or 10 mM N-acetylcysteine.
Palmero
M
M
Department I of Optics, University of Alicante, Aptdo. 99, E-3080, Alicante, Spain.
Bellot
J L
JL
Castillo
M
M
García-Cabanes
C
C
Miquel
J
J
Orts
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D007425:Intracellular Membranes / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology*; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012171:Retinal Vessels
D000975:Antioxidants; D000255:Adenosine Triphosphate