title: CD34: to select or not to select? That is the question.
abstract: Recent evidence suggests that expression of CD34 on the cell membrane does not always correlate with stem cell activity. In the mouse, there is a highly quiescent population of stem cells that lacks CD34 expression, but has full reconstituting capacity. The current review addresses the discovery of a similar population of dormant CD34-negative human hematopoietic stem cells. This information casts some uncertainty on the benefits of CD34+ cell isolation for stem cell transplantation, until more is known about the novel CD34-negative stem cell population. Methods designed to achieve removal of specific mature blood cell lineages might prove to be most advantageous in the future.
authors.affiliation: Children's Hospital Los Angeles, Dept Pediatrics, University of Southern California School of Medicine, 90027, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Dao
authors.forename: J A
authors.initials: JA
authors.lastname: Nolta
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34 / Q000032:analysis*; D002469:Cell Separation / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D051379:Mice; D014183:Transplantation, Heterologous
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34