title: Short-term tracheal occlusion in fetal lambs with diaphragmatic hernia improves lung function, even in the absence of lung growth.
abstract: OBJECTIVE
  Prolonged tracheal occlusion (TO) accelerates lung growth but impairs surfactant production. Short-term TO results in less lung growth but preserves type II cell function. The authors studied the effects of short-term TO on lung physiology in diaphragmatic hernia.
  
  
  METHODS
  Diaphragmatic hernia was created in 9 fetal lambs at 90 to 95 days. Five were left uncorrected (CDH), 4 underwent 2-week TO (108 to 122 days; CDH + TO). Five unoperated lambs served as controls. Near-term (136 days) fetuses were ventilated for 90 to 150 minutes. Pulmonary arterial pressure, postductal blood gases, quasistatic compliance, total lung capacity (TLC), and lung weight to body weight (LW/BW) were measured.
  
  
  RESULTS
  There was an overall survival rate of 89% at full term. Short-term occlusion did not induce lung growth (TLC and LW/BW, 6.07 +/- 2.92 mL/kg and 0.022 +/- 0.008 in CDH, 4.86 mL/kg and 0.019 +/- 0.005 in CDH + TO, 10.81 +/- 3.55 mL/kg and 0.036 +/- 0.006 in controls, respectively). However, pulmonary hypertension in CDH (47.4 +/- 12.32/35.8 +/- 12.19 torr) was corrected by short-term occlusion (20.2 +/- 4.0/16.0 +/- 4.8 torr in CDH + TO, P< .05, single-factor analysis of variance [ANOVA]; similar to control). Best pO2 and pCO2 improved after occlusion (CDH, 48.6 +/- 6.7 torr and 107.1 +/- 34.3 torr, respectively; CDH + TO, 101.5 +/- 16.3 torr and 81.9 +/- 2.4 torr; control, 291.4 +/- 4.7 torr and 37.7 +/- 17.3), as did oxygenation index (P < .05, CDH vCDH + TO; CDH, 97.2 +/- 23.0; CDH + TO, 28.7 +/- 3.1; control, 5.6 +/- 0.6).
  
  
  CONCLUSIONS
  Short-term TO corrects pulmonary hypertension and improves gas exchange in fetal lambs with diaphragmatic hernia despite failure to produce accelerated lung growth. Inducing lung maturation and correcting the physiological derangement in diaphragmatic hernia may be more important than achieving lung growth alone.
authors.affiliation: Department of Pathology, Brown University School of Medicine and Hasbro Children's Hospital, Providence, RI, USA.
authors.forename: Y K
authors.initials: YK
authors.lastname: Wild
authors.forename: G J
authors.initials: GJ
authors.lastname: Piasecki
authors.forename: M E
authors.initials: ME
authors.lastname: De Paepe
authors.forename: F I
authors.initials: FI
authors.lastname: Luks
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001795:Blood Pressure Determination; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus; D006548:Hernia, Diaphragmatic / Q000503:physiopathology*; D008168:Lung / Q000254:growth & development / Q000503:physiopathology*; D010101:Oxygen Consumption; D011247:Pregnancy; D011270:Pregnancy, Animal; D011336:Probability; D012016:Reference Values; D012129:Respiratory Function Tests; D012756:Sheep; D014135:Tracheal Stenosis / Q000503:physiopathology*