title: Growth inhibition of Ph+ progenitor cells from CML patients using the tyrosine kinase inhibitor CGP57148B.
abstract: BACKGROUND
  Different methods have been investigated for their purging capacity of contaminating CML cells in autologous stem cell products. CGP57148B, a tyrphostin, has been shown to be efficient in the reduction of cell proliferation of CML cell lines and primary CML cells, as well as in the inhibition of bcr/abl-related tumor formation in animal models.
  
  
  METHODS
  The effect of CGP57148B on purified CD34+ progenitor cells from BM, PB, or leukapheresis products of 8 CML patients was studied under serum-free cytokine-supplemented ex vivo culture conditions.
  
  
  RESULTS
  FISH as well as RT-PCR analysis showed a significant reduction of Ph+ cells after 7 days ex vivo-culture in the presence of the tyrphostin. Growth of Ph- cells was almost unaffected by treatment with CGP57148B.
  
  
  CONCLUSIONS
  Our results support the observation that CGP57148B can selectively inhibit proliferation of Ph+/bcr/abl+ primary CML cells under serum-free cytokine-supplemented culture conditions.
authors.affiliation: Department of Internal Medicine I-Hematology/Oncology-, Albert-Ludwigs-University, Freiburg Medical Center, Germany.
authors.forename: C F
authors.initials: CF
authors.lastname: Waller
authors.forename: M
authors.initials: M
authors.lastname: Ali
authors.forename: M
authors.initials: M
authors.lastname: Heinzinger
authors.forename: W
authors.initials: W
authors.lastname: Lange
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D000970:Antineoplastic Agents / Q000633:toxicity*; D001549:Benzamides; D001854:Bone Marrow Cells / Q000473:pathology; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D000068877:Imatinib Mesylate; D017404:In Situ Hybridization, Fluorescence; D007937:Leukapheresis; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D010879:Piperazines / Q000633:toxicity*; D011743:Pyrimidines / Q000633:toxicity*
chemical_list: D018952:Antigens, CD34; D000970:Antineoplastic Agents; D001549:Benzamides; D016895:Culture Media, Serum-Free; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate