T-cell epitope analysis of Mag 3, an important allergen from the house dust mite, Dermatophagoides farinae.
Here we describe the detection of T-cell epitope region on the house dust mite allergen Mag 3, which has been shown to trigger T-cell proliferation in mite-allergic asthmatic patients. We first examined murine T-cell epitope using T-cell fraction prepared from recombinant Mag 3 (r-Mag 3)-primed H-2k mice. Initial proliferation assay with truncated r-Mag 3 indicated that N-terminal 113 amino acid region was required for triggering T-cell activation. Subsequent epitope scanning with synthetic overlapping peptides revealed that T-cell reactive region was assigned within amino acid range 56-75. We also explored human T-cell determinant using specific T-cells from mite-allergic patients. Intriguingly, we found that amino acid range 56-85, a portion partially overlapping with that identified in r-Mag 3-primed mice, was exclusively recognized by T-cells from different patients. Further investigation of unique T-cell epitope region found in this study would provide insight into the development of animal therapeutic model and/or peptide vaccine for asthma.
Kawamoto
S
S
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan. skawa@ipc.hiroshima-u.ac.jp
Ohno
K
K
Tategaki
A
A
Aki
T
T
Shigeta
S
S
Jyo
T
T
Suzuki
O
O
Ono
K
K
D000485:Allergens / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D004391:Dust; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000009:adverse effects / Q000235:genetics / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology; D006798:Housing; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008925:Mites / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D004391:Dust; D018984:Epitopes, T-Lymphocyte; D006023:Glycoproteins; D006183:H-2 Antigens; D010455:Peptides; D011993:Recombinant Fusion Proteins