title: Intracellular calcium signalling in magnocellular neurones of the rat supraoptic nucleus: understanding the autoregulatory mechanisms.
abstract: Oxytocin and vasopressin, released at the soma and dendrites of neurones, bind to specific autoreceptors and induce an increase in [Ca2+]i. In oxytocin cells, the increase results from a mobilisation of Ca2+ from intracellular stores, whereas in vasopressin cells, it results mainly from an influx of Ca2+ through voltage-dependent channels. The response to vasopressin is coupled to phospholipase C and adenylyl-cyclase pathways which are activated by V1 (V1a and V1b)- and V2-type receptors respectively. Measurements of [Ca2+]i in response to V1a and V2 agonists and antagonists suggest the functional expression of these two types of receptors in vasopressin neurones. The intracellular mechanisms involved are similar to those observed for the action of the pituitary adenylyl-cyclase-activating peptide (PACAP). Isolated vasopressin neurones exhibit spontaneous [Ca2+]i oscillations and these are synchronised with phasic bursts of electrical activity. Vasopressin modulates these spontaneous [Ca2+]i oscillations in a manner that depends on the initial state of the neurone, and such varied effects of vasopressin may be related to those observed on the electrical activity of vasopressin neurones in vivo.
authors.affiliation: UPR 9055-CNRS, Biologie des Neurones Endocrines, Montpellier, France. gdaya@bacchus.montp.inserm.fr
authors.forename: G
authors.initials: G
authors.lastname: Dayanithi
authors.forename: N
authors.initials: N
authors.lastname: Sabatier
authors.forename: H
authors.initials: H
authors.lastname: Widmer
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D006706:Homeostasis / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D010121:Oxytocin / Q000502:physiology; D051381:Rats; D013495:Supraoptic Nucleus / Q000166:cytology / Q000502:physiology*; D014667:Vasopressins / Q000502:physiology
chemical_list: D014667:Vasopressins; D010121:Oxytocin