Axonal transport of TREK and TRAAK potassium channels in rat sciatic nerves.
The recent cloning, functional expression and brain localization of two new potassium channels, TREK and TRAAK, led us to examine whether both channels are present in peripheral nerves and can move along axons by means of axonal transport mechanisms. Using specific antibodies directed against TREK and TRAAK peptides, we found that immunoreactivity for both potassium channels accumulates above and below a ligature in rat sciatic nerves. The process was rapid and bidirectional suggesting that the channels are associated with vesicles. This represents the first report on the axonal transport of potassium channels.
Bearzatto
B
B
Laboratory of Neurophysiology, Université Libre de Bruxelles, Belgium.
Lesage
F
F
Reyes
R
R
Lazdunski
M
M
Laduron
P M
PM
D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D001369:Axons / Q000378:metabolism; D007150:Immunohistochemistry; D008026:Ligation; D015221:Potassium Channels / Q000378:metabolism*; D024683:Potassium Channels, Tandem Pore Domain; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000166:cytology* / Q000378:metabolism*
C503434:Kcnk4 protein, rat; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; C103742:potassium channel protein TREK-1