title: Cytological and immunological changes in bronchoalveolar lavage fluid and histological observation of lung lesions in pigs immunized with Mycoplasma hyopneumoniae inactivated vaccine prepared from broth culture supernate.
abstract: We have compared the cytology of bronchoalveolar lavage fluid (BALF) and the pathology of lung lesions in pigs immunized with/without Mycoplasma hyopneumoniae inactivated vaccine prepared from broth culture supernate on experimental infection. Numbers of total cells, macrophages, neutrophils and lymphocytes have decreased in BALF of vaccinated pigs following infection. The mean percentage of lung lesions, inflammatory cell infiltration into the airways and T cells accumulation around the bronchi were reduced in vaccinated pigs. The levels of tumor necrosis factor (TNF)-alpha also decreased in vaccinated pigs. These results suggest that the vaccination may contribute to decrease TNF-alpha production, and therefore, inflammatory cell responses in the lung due to M. hyopneumoniae infection were suppressed, resulting in fewer lung lesions.
authors.affiliation: Zen-noh Institute of Animal Health, Sakura, Japan. okadamu@zk.zennoh.or.jp
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Asai
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Sakano
authors.forename: S
authors.initials: S
authors.lastname: Sato
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology* / Q000276:immunology* / Q000382:microbiology; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D008168:Lung / Q000276:immunology* / Q000382:microbiology / Q000473:pathology*; D009174:Mycoplasma / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000276:immunology / Q000473:pathology; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D017077:Culture Media, Conditioned; D014409:Tumor Necrosis Factor-alpha; D015164:Vaccines, Inactivated; D014765:Viral Vaccines