Use of anion exchange resin-packed capillary column for rapid detection of anti-double-stranded DNA antibody in systemic lupus erythematosus serum.
An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point. A linear anti-dsDNA antibody dose-response curve was obtained between luminescence intensity and concentration of anti-dsDNA antibody in the range 25-200 IU/mL. This simple technique permits the assay of anti-dsDNA autoimmune antibodies within 7 minutes. These results give reduced detection times compared with those previously reported (Lim et al., 1999) through the use of capillary columns.
Lim
T
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
Nakamura
N
N
Matsunaga
T
T
D000469:Alkaline Phosphatase / Q000737:chemistry; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D005069:Evaluation Studies as Topic; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology*
D001323:Autoantibodies; D004247:DNA; D000469:Alkaline Phosphatase