Evaluation of the MB/BacT mycobacterium detection system for susceptibility testing of Mycobacterium tuberculosis.
The MB/BacT mycobacterium detection system was evaluated for its performance in the susceptibility testing of Mycobacterium tuberculosis. Eighty-three M. tuberculosis isolates were processed. Results for all isoniazid-, rifampin- and streptomycin-susceptible, isoniazid-resistant, and rifampin-resistant M. tuberculosis isolates with the MB/BacT system agreed 100% with those obtained by the agar proportion method. The agreements between the two methods for streptomycin- and ethambutol-resistant isolates were 96.4 and 90.4%, respectively. The susceptibility test results were obtained in 7 days, on average. These data demonstrate that the MB/BacT system is an accurate, nonradiometric method for rapid susceptibility testing of M. tuberculosis.
Díaz-Infantes
M S
MS
Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario "Gregorio Marañón,", Madrid, Spain. mjrfer@efd.net
Ruiz-Serrano
M J
MJ
Martínez-Sánchez
L
L
Ortega
A
A
Bouza
E
E
D000962:Antimalarials / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology*; D013307:Streptomycin / Q000494:pharmacology*
D000962:Antimalarials; D007538:Isoniazid; D012293:Rifampin; D013307:Streptomycin