Th2-dependent airway eosinophilia is regulated by preproenkephalin.
We have used preproenkephalin (PPNK)-deficient mice to study the role of PPNK in the development of airway inflammation. Airway eosinophilia was established by either sensitization followed by airway challenge with OVA or by infection with Nippostrongylus brasiliensis. Both models induce a strong Th2 immune response, characterized by an IL-5-dependent airway eosinophilia. We observed that although the accumulation of lymphocytes in the airways of PPNK-deficient mice was similar to that induced in control mice, IL-5 production and eosinophil infiltration were reduced. We conclude from this work that PPNK has a role in enhancing Th2 cell function and that this molecule may be an important target in asthma immunotherapy.
Hook
S
S
Malaghan Institute of Medical Research, Wellington School of Medicine, P.O. Box 7060, Wellington, New Zealand. shook@malaghan.org.nz
Prout
M
M
Camberis
M
M
Konig
M
M
Zimmer
A
A
Van Heeke
G
G
Le Gros
G
G
D000818:Animals; D000917:Antibody Formation; D016207:Cytokines / Q000096:biosynthesis; D004745:Enkephalins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D004802:Eosinophilia / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D008171:Lung Diseases / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D011498:Protein Precursors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology*
D016207:Cytokines; D004745:Enkephalins; D011498:Protein Precursors; C033660:preproenkephalin