[A noninstrumental Immunoassay based on colloidal dyes].
Detecting labels based on water dispersions of colloidal textile dyes were developed that are useful in various analytical and diagnostic test systems for a simple visual assessment of the assay. Colored water-insoluble particles of dyes were used for the sorptional immobilization of streptavidin on their surface. The resulting streptavidin-dye (STR-DYE) complexes possessed a high visualizing capacity and were used for the combined detection of pesticides (simazine and 2,4-dichlorophenoxyacetic acid) by noninstrumental immunoassay (DYE-comb-assay, competitive dot-immunoassay in the comb format). The detection limits and the duration of our DYE-comb-assay (4 ng/ml, 20-25 min), HRP-comb-assay (competitive dot-immunoassay in the comb format using the enzymic conjugate of STR with horseradish peroxidase) (16 ng/ml), and the traditional competitive ELISA (12-16 ng/ml, 1.5 h) were compared. This DYE-comb-assay is simple enough and can be used under field conditions.
Liubavina
I A
IA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. alezin@ibch.siobc.ras.ru
Salomatina
I S
IS
Zinchenko
A A
AA
Zherdeev
A V
AV
Dzantiev
B B
BB
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis* / Q000276:immunology; D003102:Colloids; D007118:Immunoassay / Q000379:methods*; D012680:Sensitivity and Specificity; D012839:Simazine / Q000032:analysis* / Q000276:immunology
D003102:Colloids; D015084:2,4-Dichlorophenoxyacetic Acid; D012839:Simazine