Allopregnanolone attenuates kainate-induced toxicity in primary cortical neurons and PC12 neuronal cells.
In this study, we demonstrated that allopregnanolone at 500 and 1000 nM significantly inhibited kainate-induced lactate dehydrogenase release from primary cortical cells by ca. 25 and 50%, respectively. Furthermore, allopregnanolone doses of 100 and 500 nM decreased cytotoxic effects of kainate (150 microM, 24-hour exposure) in PC12 neuronal cells by about 55 and 37%, respectively. These data strongly support neuroprotective effects of allopregnanolone observed in vivo.
Kajta
M
M
Department of Endocrinology, Institute of Pharmacology, Polish Academy of Sciences, Kraków.
Budziszewska
B
B
Lasoń
W
W
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists; D007608:Kainic Acid; D007770:L-Lactate Dehydrogenase / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells / Q000187:drug effects / Q000201:enzymology; D011280:Pregnanolone / Q000494:pharmacology*; D051381:Rats
D018690:Excitatory Amino Acid Agonists; D018696:Neuroprotective Agents; D011280:Pregnanolone; D007770:L-Lactate Dehydrogenase; D007608:Kainic Acid