title: Ancient DNA fragments longer than 300 bp.
abstract: It is widely assumed that ancient DNA (aDNA) extracts contain no authentic templates longer than 300 bp. Here we present results which show that fragments of up to 800 bp in length can be reproducibly amplified from aDNA extracts. The amplification involves the short tandem repeat (STR) locus HUMVWA31A. Authentication of the amplified fragments is carried out by measures of expectancy.
authors.affiliation: Institute of Zoology and Anthropology, University of Göttingen.
authors.forename: K
authors.initials: K
authors.lastname: Haack
authors.forename: S
authors.initials: S
authors.lastname: Hummel
authors.forename: B
authors.initials: B
authors.lastname: Hummel
mesh_terms: D004247:DNA / Q000235:genetics*; D018732:Forensic Anthropology; D049671:History, 18th Century; D049690:History, Ancient; D006801:Humans; D010164:Paleopathology; D010446:Peptide Fragments / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D020080:Tandem Repeat Sequences / Q000235:genetics
chemical_list: D010446:Peptide Fragments; D004247:DNA