title: Effect of L-methioninyl adenylate on the level of aminoacylation in vivo of tRNA(Met) from Escherichia coli K12.
abstract: In cells of E.coli K12 grown exponentially in minimal medium, tRNA(met), tRNA(leu) and tRNA(ile) are aminoacylated at 100%, 80% and 64%, respectively. On addition of L-methioninyl adenylate to the growth medium, one observes a specific deacylation of tRNA(met). When more than 35% of tRNA(met) is deacylated, growth rate is reduced and becomes proportional to the amount of methionyl-tRNA formed.
authors.forename: D
authors.initials: D
authors.lastname: Cassio
authors.forename: Y
authors.initials: Y
authors.lastname: Mathien
mesh_terms: D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012355:RNA, Transfer, Ile / Q000378:metabolism; D012356:RNA, Transfer, Leu / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000378:metabolism*
chemical_list: D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012355:RNA, Transfer, Ile; D012356:RNA, Transfer, Leu; D012358:RNA, Transfer, Met; C011501:methioninyl adenylate; D000249:Adenosine Monophosphate; D008715:Methionine