title: Functional synapse formation between rat olfactory receptor neurons and olfactory bulb neurons in vitro.
abstract: To explore the mechanism of the synapse formation between olfactory receptor neurons and neurons in the olfactory bulb, blocks of olfactory epithelium and slices of olfactory bulb were cocultured by a modified slice culture method. After 4 days in culture, neuron specific fibers from the olfactory epithelium block extending to the olfactory bulb slice were observed. After approximately 20 days in culture, application of forskolin, which induces excitatory responses in olfactory receptor neurons, to the epithelium induced inward current responses in olfactory bulb neurons under the voltage clamp conditions, indicating that functional synapses between olfactory receptor neurons and olfactory bulb neurons had been established in vitro conditions.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kanaki
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Kashiwayanagi
mesh_terms: D000818:Animals; D018920:Coculture Techniques; D005576:Colforsin / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000502:physiology*; D009831:Olfactory Mucosa / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology*
chemical_list: D005576:Colforsin