Eukaryotic translation initiation: there are (at least) two sides to every story.
The eukaryotic cap and poly(A) tail binding proteins, eIF4E and Pab1p, play important roles in the initiation of protein synthesis. The recent structures of the complex of eIF4E bound to the methylated guanosine (cap) found at the 5'end of messenger RNA (mRNA), the complex of eIF4E bound to peptide fragments of two related translation factors (eIF4G and 4E-BP1), and the complex of the N-terminal fragment of Pab1p bound to polyadenylate RNA have revealed that eIF4E and Pab1p contain at least two distinct functional surfaces. One surface is used for binding mRNA, and the other for binding proteins involved in translation initiation.
Sachs
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Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA. asachs@uclink4.berkeley.edu
Varani
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D000595:Amino Acid Sequence; D000818:Animals; D005057:Eukaryotic Cells; D039561:Eukaryotic Initiation Factor-4E; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D008958:Models, Molecular; D010448:Peptide Initiation Factors / Q000737:chemistry* / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D039102:Poly(A)-Binding Proteins; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000502:physiology*; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*
D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; C111106:7-methylguanosine 5'-diphosphate; D006153:Guanosine Diphosphate; D011061:Poly A