[Interference by D-mannitol on serum D-arabinitol determination by enzymatic assay].
We examined a disparity in D-arabinitol values between two commercial assay kits, LABOFIT and ARABINITEC-AUTO. The determined values by the former were increased by 26%(y = 0.2643x) because of concomitant D-mannitol, whereas those by our newly developed ARABINITEC-AUTO was increased only by 2%(y = 0.0242x). Of 109 samples, 5 samples were found to contain more than 100 mumol/l of D-mannitol. A clear relation(r = 0.89) was noted between the degree of disparity between measurements by the two methods and D-mannitol concentrations in samples. Thus, we have proved that the disparity is mainly caused by D-mannitol.
Matsuda
Y
Y
Marukin Shoyu Co., Ltd., Kyoto.
Ohta
Y
Y
Tsukada
Y
Y
Nishikiori
H
H
Sakai
T
T
Yuasa
S
S
Fujita
N
N
Yoshimura
M
M
D015415:Biomarkers / Q000097:blood; D002177:Candidiasis / Q000175:diagnosis; D006801:Humans; D008353:Mannitol; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods; D013402:Sugar Alcohols / Q000097:blood*
D015415:Biomarkers; D011933:Reagent Kits, Diagnostic; D013402:Sugar Alcohols; D008353:Mannitol; C014999:arabitol