title: On-Bead Quantitation of Resin Bound Functional Groups Using Analogue Techniques with Vibrational Spectroscopy.
abstract: This report introduces two novel methodologies for the on-bead, functional group quantitation of solid phase synthesis using vibrational spectroscopy. "Analogue bleed" quantitation is an infrared spectroscopic technique in which combinatorial library bead spectral data are regressed against solution phase functional analogue spectra of a ligand in question. "Dual Analogue" quantitation is introduced as an infrared or Raman technique in which ligand-to-styrene band ratios of solution phase ligand/styrene monomer standards are used to generate an internally path length referenced calibration which can then be applied to solvent swollen beads. These technologies have application across a wide range of functional groups and can be applied throughout each step of a resin bound synthesis.
authors.affiliation: AstraZeneca Pharmaceuticals, A Business Unit of Zeneca Inc., 1800 Concord Pike, Wilmington, Delaware 19850.
authors.initials: DE
authors.lastname: Pivonka