Concurrent administration of fish meal and sodium nitrite does not promote renal carcinogenesis in rats after initiation with N-ethyl-N-hydroxyethylnitrosamine.
The modifying effects of concurrent administration of fish meal and sodium nitrite on the development of renal tumors after initiation with N-ethyl-N-hydroxyethylnitrosamine (EHEN) were investigated. A total of 120 male 6-week-old Wistar rats were divided into six groups. Groups 1-3 (30 animals each) were given 1000 ppm EHEN in their drinking water for 3 weeks as an initiation treatment for renal cancer induction and thereafter fed respective diets containing 64, 32, and 8% (original concentration in the basal diet) fish meal, and simultaneously given 0.12% sodium nitrite in the drinking water for 33 weeks. Groups 4-6 (ten animals each) were similarly treated without the prior application of EHEN. At the end of the 37th experimental week, all surviving animals were autopsied and examined histopathologically for the existence of renal proliferative lesions. The incidences of dysplastic lesions, adenomas or adenocarcinomas of the kidney were not significantly different among groups 1-3. No renal proliferative lesions were found in groups 4-6. Chronic nephropathy was slightly but significantly enhanced in the 64 and 32% fish meal-treated groups as compared with group 3. Our results suggest that concurrent administration of fish meal and sodium nitrite does not affect the post-initiation phase of EHEN-induced renal carcinogenesis in the rat.
Furukawa
F
F
Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, Japan.
Nishikawa
A
A
Miyauchi
M
M
Nakamura
H
H
Son
H Y
HY
Yamagishi
M
M
Hirose
M
M
D000230:Adenocarcinoma / Q000139:chemically induced; D000236:Adenoma / Q000139:chemically induced; D000311:Adrenal Glands / Q000187:drug effects; D000818:Animals; D001835:Body Weight; D003043:Cocarcinogenesis; D004032:Diet; D004052:Diethylnitrosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D005396:Fish Products; D007668:Kidney / Q000187:drug effects; D007680:Kidney Neoplasms / Q000139:chemically induced*; D008099:Liver / Q000187:drug effects; D008297:Male; D009929:Organ Size; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012977:Sodium Nitrite / Q000008:administration & dosage* / Q000633:toxicity*; D013997:Time Factors
D018809:Proliferating Cell Nuclear Antigen; C023845:N-ethyl-N-hydroxyethylnitrosamine; D004052:Diethylnitrosamine; D012977:Sodium Nitrite