title: Expression of N-acetylglucosaminyltransferase V in colorectal cancer correlates with metastasis and poor prognosis.
abstract: N-Acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes beta 1-6 branching of N-acetylglucosamine on asparagine-linked oligosaccharides of cell proteins. Metastatic potential of various cancer cells has been shown to correlate with increase of GnT-V activity and concomitant beta 1-6 branching of N-acetylglucosamine. However, protein expression of GnT-V in human cancer tissue and its clinical significance have not yet been demonstrated. To clarify the possible relationship between metastasis and GnT-V in human colorectal cancer, protein expression of GnT-V was studied using surgically resected specimens. We established a monoclonal antibody against GnT-V and performed immunohistochemical analysis of 103 human colorectal cancer cases. Of 103 cases, 26 cases (25.2 %) showed specific expression of GnT-V in colorectal cancer tissues. The expression of GnT-V was significantly correlated with distant metastasis (P < 0.05, chi2 test). Overall 5-year survival rate was 52.8% for GnT-V-positive patients and 81.7% for GnT-V-negative patients (P < 0.01, Log-rank test). We showed direct evidence for the relationship between GnT-V and metastasis in human colorectal cancer. Screening of GnT-V expression in colorectal cancer may provide useful information for prognosis of postoperative patients.
authors.affiliation: Department of Surgical Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari, Japan. kmurata@yo.rim.or.jp
authors.forename: K
authors.initials: K
authors.lastname: Murata
authors.forename: E
authors.initials: E
authors.lastname: Miyoshi
authors.forename: M
authors.initials: M
authors.lastname: Kameyama
authors.forename: O
authors.initials: O
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Kabuto
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Hiratsuka
authors.forename: H
authors.initials: H
authors.lastname: Ohigashi
authors.forename: S
authors.initials: S
authors.lastname: Ishiguro
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Honda
authors.forename: F
authors.initials: F
authors.lastname: Takemura
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
authors.forename: S
authors.initials: S
authors.lastname: Imaoka
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008875:Middle Aged; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D015996:Survival Rate
chemical_list: D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase