title: Short report: phylogenetically distinct hepatitis E viruses in Pakistan.
abstract: Hepatitis E, which is enterically transmitted, is the most common cause of acute hepatitis in much of Asia. Phylogenetic analysis of several isolates of hepatitis E virus (HEV) from Asia suggests that transmission of this virus is geographically restricted. In Sarghoda, Pakistan, HEV Sar-55 was isolated from a 1987 outbreak. It belongs to the Central-Asian cluster of the Asian sub-genotype. We now report the complete sequence of a second Pakistan HEV from a 1988 outbreak in Abbottabad. The Abbottabad nucleotide sequence was compared with 15 other complete HEV sequences using statistical methods of phylogenetic analysis. The analysis showed that Abbottabad HEV belongs to the South Asia cluster of the Asian sub-genotype. The sequence differences of the 2 Pakistan isolates recovered only one year apart suggest that HEV of 2 distinct origins circulate in Pakistan.
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, District of Columbia 20307-5100, USA.
authors.forename: H
authors.initials: H
authors.lastname: van Cuyck-Gandré
authors.forename: H Y
authors.initials: HY
authors.lastname: Zhang
authors.forename: S A
authors.initials: SA
authors.lastname: Tsarev
authors.forename: R L
authors.initials: RL
authors.lastname: Warren
authors.forename: J D
authors.initials: JD
authors.lastname: Caudill
authors.forename: N J
authors.initials: NJ
authors.lastname: Snellings
authors.forename: L
authors.initials: L
authors.lastname: Bégot
authors.forename: B L
authors.initials: BL
authors.lastname: Innis
authors.forename: C F
authors.initials: CF
authors.lastname: Longer
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D016751:Hepatitis E / Q000453:epidemiology / Q000209:etiology / Q000821:virology*; D016752:Hepatitis E virus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010154:Pakistan / Q000453:epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary