title: Neurons and ECM regulate occludin localization in brain endothelial cells.
abstract: We report that extracellular matrix and neurons modulate the expression of occludin, one of the main components of tight junctions, by rat brain endothelial cells (RBE4.B). Of the three extracellular matrix proteins which we tested (collagen I, collagen IV, and laminin), collagen IV stimulated at the best the expression of occludin mRNA. The corresponding protein, however, was not synthesized. Significant amounts of occludin accumulated only when RBE4.B cells were cultured on collagen IV-coated inserts, in the presence of cortical neurons, plated on laminin-coated companion wells. Finally, occludin segregated at the cell periphery, only when endothelial cells were co-cultured with neurons for > or = 1 week.
authors.affiliation: Istituto di Neuropsichiatria, Policlinico Universitario Paolo Giaccone, Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Savettieri
authors.forename: I
authors.initials: I
authors.lastname: Di Liegro
authors.forename: C
authors.initials: C
authors.lastname: Catania
authors.forename: L
authors.initials: L
authors.lastname: Licata
authors.forename: G L
authors.initials: GL
authors.lastname: Pitarresi
authors.forename: S
authors.initials: S
authors.lastname: D'Agostino
authors.forename: G
authors.initials: G
authors.lastname: Schiera
authors.forename: V
authors.initials: V
authors.lastname: De Caro
authors.forename: G
authors.initials: G
authors.lastname: Giandalia
authors.forename: L I
authors.initials: LI
authors.lastname: Giannola
authors.forename: A
authors.initials: A
authors.lastname: Cestelli
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000502:physiology; D003094:Collagen / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005333:Fetus; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D007797:Laminin / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D062793:Occludin; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D008565:Membrane Proteins; D016900:Neurofilament Proteins; D062793:Occludin; C568987:Ocln protein, rat; D012333:RNA, Messenger; D003094:Collagen