Mass transport limitations reduce the effective stereospecificity in enzyme-catalyzed kinetic resolution.
[reaction-see text] The kinetic resolution of seudenol catalyzed by Candida antarctica lipase B in hexane was investigated. Large differences in reaction rate and stereospecificity were observed when different enzyme preparations were used. These differences were ascribed to mass transport limitations which reduced both reaction rate and stereospecificity. Lyophilized enzyme preparations were more apt to give this problem than immobilized preparations. Further, low substrate concentrations enhanced the effect. Thus, high alcohol concentrations and enzyme immobilization can be recommended.
Rotticci
D
D
Department of Chemistry, Organic Chemistry, and Department of Biotechnology, Royal Institute of Technology, SE-100 44 Stockholm, Sweden.
Norin
T
T
Hult
K
K
D002175:Candida / Q000201:enzymology; D004798:Enzymes / Q000378:metabolism*; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
D004798:Enzymes; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; C093667:Novozyme 435; D008049:Lipase