The effect of bisulfite modification on the template activity of DNA for DNA polymerase I.
Cytosine residues of poly(C) and heat-denatured calf thymus DNA were transformed into 5,6-dihydrouracil-6-sulfonate (U(SO(-) (3))) residues by treatment with bisulfite. The poly(U(SO(-) (3))(2), C(3)) and poly(U(SO(-) (3))(9), C(1)) prepared did not form inter-base binding with either poly(A) or poly(I) as judged by the absence of hypochromicity in ultraviolet absorbance. U(SO(-) (3)) residues in the DNA inactivated it to serve as template for E.coli DNA polymerase I, while the template activity was restored by conversion of the U(SO(-) (3)) residues into U.
Kai
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Tsuruo
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Hayatsu
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D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D066298:In Vitro Techniques; D016296:Mutagenesis; D011061:Poly A / Q000378:metabolism; D011066:Poly C / Q000737:chemistry / Q000378:metabolism; D011069:Poly I / Q000378:metabolism; D011072:Poly U / Q000737:chemistry / Q000378:metabolism; D013447:Sulfites / Q000633:toxicity*
D013447:Sulfites; D011061:Poly A; D011069:Poly I; C035610:poly(U-C); D011072:Poly U; D011066:Poly C; D004247:DNA; D004256:DNA Polymerase I