A spontaneous murine melanoma lung metastasis comprised of host x tumor hybrids.
Cells from a lung metastasis, arising from Cloudman S91 melanoma cells implanted s.c. in the tail of a BALB/c nu/nu mouse, were comprised chiefly of host x tumor hybrids. These lung metastasis cells showed: (a) 30-40% increased DNA content; (b) resistance to 10(-4) M hypoxanthine, 4 x 10(-7) M aminopterin, and 1.6 x 10(-5) M thymidine (HAT) + G418; and (c) the presence in genomic DNA of genes for both wt and albino tyrosinase, reflecting the DBA/2J (Cloudman S91) and BALB/c mouse genotypes, respectively. Individual clones of lung metastasis cells expressed enhanced pigmentation, motility, and responsiveness to MSH/IBMX, a behavior similar to that recently reported for artificially generated melanoma x macrophage fusion hybrids. These similarities suggested that the host fusion partner generating the lung metastasis hybrids might have been a macrophage, although formal proof for this was not possible. The results provide the first direct evidence that host x tumor hybridization could serve as an initiating mechanism for melanoma metastasis.
Chakraborty
A K
AK
Department of Dermatology, Yale School of Medicine, New Haven, Connecticut 06520, USA.
Sodi
S
S
Rachkovsky
M
M
Kolesnikova
N
N
Platt
J T
JT
Bolognia
J L
JL
Pawelek
J M
JM
D000630:Aminopterin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015703:Antigens, CD / Q000378:metabolism; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D002465:Cell Movement; D002633:Chemotaxis; D005434:Flow Cytometry; D005839:Gentamicins / Q000494:pharmacology; D019271:Hypoxanthine / Q000494:pharmacology; D015151:Immunoblotting; D008175:Lung Neoplasms / Q000556:secondary* / Q000648:ultrastructure; D051907:Lysosomal Membrane Proteins; D008545:Melanoma / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009368:Neoplasm Transplantation; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013936:Thymidine / Q000494:pharmacology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000900:Anti-Bacterial Agents; D015703:Antigens, CD; D005839:Gentamicins; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D019271:Hypoxanthine; C010680:antibiotic G 418; D014442:Monophenol Monooxygenase; D000630:Aminopterin; D013936:Thymidine