Experimental conditions that increase the production of HIV-1 by monocyte-derived macrophages: use of collagen matrix.
Monocyte-derived macrophages (MDMs) from healthy blood donors were isolated by adherence to tissue culture-treated plasticware. They were cultured in vitro in medium supplemented with human serum and recombinant GM-CSF, then infected with the macrophage-tropic prototype strain HIV-1-PAR. Virus production was quantitated at various times after infection by measuring reverse transcriptase concentration in cell-free tissue culture supernatant fluids, using a sensitive nonradioactive assay. Virus production was significantly increased by culturing MDMs on plasticware previously coated with collagen 1. The increase in virus production was dependent upon collagen 1 concentration, with maximal value being encountered after coating with 1.5 microg/cm2. These results indicate that the sensitivity of peripheral macrophages to HIV-1 infection might be influenced by contact-dependent interactions involving components of the extracellular matrix that take place during the process of monocyte extravasation and migration.
Godard
C M
CM
Inserm Unité 322, Parc Scientifique et Technologique de Luminy, Marseille, France.
Chermann
J C
JC
D001798:Blood Proteins / Q000494:pharmacology; D002469:Cell Separation; D003094:Collagen / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015497:HIV-1 / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology / Q000821:virology*; D009000:Monocytes; D010969:Plastics / Q000494:pharmacology; D011994:Recombinant Proteins; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication / Q000276:immunology
D001798:Blood Proteins; D010969:Plastics; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003094:Collagen