title: MutS homolog 4 localization to meiotic chromosomes is required for chromosome pairing during meiosis in male and female mice.
abstract: Msh4 (MutS homolog 4) is a member of the mammalian mismatch repair gene family whose members are involved in postreplicative DNA mismatch repair as well as in the control of meiotic recombination. In this report we show that MSH4 has an essential role in the control of male and female meiosis. We demonstrate that MSH4 is present in the nuclei of spermatocytes early in prophase I and that it forms discrete foci along meiotic chromosomes during the zygotene and pachytene stages of meiosis. Disruption of the Msh4 gene in mice results in male and female sterility due to meiotic failure. Although meiosis is initiated in Msh4 mutant male and female mice, as indicated by the chromosomal localization of RAD51 and COR1 during leptonema/zygonema, the chromosomes fail to undergo normal pairing. Our results show that MSH4 localization on chromosomes during the early stages of meiosis is essential for normal chromosome synapsis in prophase I and that it acts in the same pathway as MSH5.
authors.affiliation: Department of Cell Biology, Albert Einstein College of Medicine, The Bronx, New York 10461 USA.
authors.forename: B
authors.initials: B
authors.lastname: Kneitz
authors.forename: P E
authors.initials: PE
authors.lastname: Cohen
authors.forename: E
authors.initials: E
authors.lastname: Avdievich
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.forename: M F
authors.initials: MF
authors.lastname: Kane
authors.forename: H
authors.initials: H
authors.lastname: Hou
authors.forename: R D
authors.initials: RD
authors.lastname: Kolodner
authors.forename: R
authors.initials: R
authors.lastname: Kucherlapati
authors.forename: J W
authors.initials: JW
authors.lastname: Pollard
authors.forename: W
authors.initials: W
authors.lastname: Edelmann
mesh_terms: D000818:Animals; D020137:Base Pair Mismatch; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics; D005314:Embryonic and Fetal Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007247:Infertility, Female / Q000235:genetics; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D051135:Rad51 Recombinase
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C409221:MSH4 protein, human; C502328:Msh4 protein, mouse; D011506:Proteins; D051135:Rad51 Recombinase; C495154:Rad51 protein, mouse