title: Ultrastructural study of the remnant liver after extensive hepatectomy in dogs; especially morphological alterations of sinusoidal endothelial cells.
abstract: OBJECTIVE
  To investigate the role of sinusoidal endothelial cells in the mechanism of the remnant liver dysfunction after extensive hepatectomy, morphological alteration of sinusoidal endothelial cells and cytoprotective effects of prostaglandin I2 analog were studied by ultrastructural analysis.
  
  
  METHODS
  Mongrel dogs (n = 24) were divided into 3 groups according to the extent of hepatectomy and the use of prostaglandin I2 analog preoperatively. Group 1: 70% hepatectomy (n = 8), Group 2: 84% hepatectomy (n = 8), and Group 3: 84% hepatectomy with administration of prostaglandin I2 analog, preoperatively (n = 8). Morphology of hepatic sinusoidal endothelial cells was examined after hepatectomy in all cases.
  
  
  RESULTS
  Group 2: Enlargement of the sinusoidal endothelial fenestrations was transitorily occurred immediately after hepatectomy, but were dramatically decreased in size 24 h after the operation. Most sinusoidal endothelial cells were destroyed and separated from the sinusoidal wall. Fibrin deposits and sludge were found in the Disse's space and sinusoidal lumens, resembling liver failure at 24 h after the operation. Group 1 and 3: sinusoidal endothelial fenestrations remained slightly enlarged at 24 h after the operation, but fibrin deposits or sludge were not observed in sinusoidal lumens.
  
  
  CONCLUSIONS
  Our data suggest that extensive hepatectomy changed the morphology of sinusoidal endothelial cells. Administration of prostaglandin I2 analog helped maintain structural integrity of sinusoidal endothelial cells, improved hepatic microcirculation and thus served to reduce remnant liver dysfunction.
authors.affiliation: First Department of Surgery, Mie University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kuroda
authors.forename: Y
authors.initials: Y
authors.lastname: Kawarada
authors.forename: B C
authors.initials: BC
authors.lastname: Das
authors.forename: M
authors.initials: M
authors.lastname: Iwata
authors.forename: S
authors.initials: S
authors.lastname: Isaji
mesh_terms: D000818:Animals; D004285:Dogs; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D011464:Epoprostenol / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D006498:Hepatectomy; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D017093:Liver Failure / Q000473:pathology*; D008111:Liver Function Tests; D008297:Male; D008855:Microscopy, Electron, Scanning; D011183:Postoperative Complications / Q000473:pathology*
chemical_list: C049453:OP 2507; D011464:Epoprostenol