title: A novel human processed gene, DAD-R, maps to 12p12 and is expressed in several organs.
abstract: A cDNA of a processed gene of human DAD-1 (defender against apoptotic cell death) was cloned from the human neuroblastoma cell line SH-SY5Y. The genomic sequence of this novel processed gene, DAD-R, lacked introns and was flanked by 8 bp terminal repeats. RT-PCR showed that the transcript is expressed predominantly in testis, ovaries, pancreas, lung and skeletal muscle. DAD-R has several possible initiation codons, one of them producing an open reading frame comprising 75% of the DAD-1 gene. We determined the chromosomal localization of DAD-R as 12p11.2-12p12.1, an area linked to familial synpolydactyly and frequently amplified in a variety of cancers, including those of testis, ovaries, pancreas and lungs.
authors.affiliation: Insitute of Biotechnology, Viikki Biocenter, P.O. Box 56 (Viikinkaari 9), FIN-00014 University of, Helsinki, Finland. tpkuitti@operoni.helsinki.fi
authors.forename: T
authors.initials: T
authors.lastname: Kuittinen
authors.forename: A
authors.initials: A
authors.lastname: Eggert
authors.forename: P
authors.initials: P
authors.lastname: Lindholm
authors.forename: N
authors.initials: N
authors.lastname: Horelli-Kuitunen
authors.forename: A
authors.initials: A
authors.lastname: Palotie
authors.forename: J M
authors.initials: JM
authors.lastname: Maris
authors.forename: M
authors.initials: M
authors.lastname: Saarma
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: C409423:DAD1P1 protein, human; D018076:DNA, Complementary; D012333:RNA, Messenger; D012097:Repressor Proteins