Angular reconstitution of the Staphylothermus marinus phosphoenolpyruvate synthase.
The processes of single particle electron crystallography and three-dimensional angular reconstitution are applied to digital cryoelectron images of a macromolecular complex, the Staphylothermus marinus phosphoenolpyruvate synthase. In particular, the application of IQAD (iterative quaternionic angular determination) is exemplified in the context of more canonical approaches.
Li
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Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
Ottensmeyer
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FP
Harauz
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D020285:Cryoelectron Microscopy / Q000379:methods*; D019620:Desulfurococcaceae / Q000201:enzymology / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008958:Models, Molecular; D017866:Phosphotransferases (Paired Acceptors) / Q000648:ultrastructure*; D011487:Protein Conformation
D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase