GALECTIN-3 expression in differentiating human myeloid cells.
Galectin-3 is an endogenous mammalian carbohydrate-binding protein with affinity for ABH group carbohydrate epitopes and polylactosamine glycans present on cell surface and extracellular matrix glycoproteins. It has been shown to play a role in cell differentiation, morphogenesis, adhesion and cell proliferation regulation. Progenitor cell proliferation in bone marrow depends on stem cell factors including those modulating their adhesion to the bone marrow stroma. The present study shows that the 32 kD galectin-3 is developmentally expressed in human myeloid cells and is strongly upregulated on the cell surface of late mature myeloid cells. Despite the fact that the expression of the galectin-3 is very low in CD34+ early myeloid cell, a 70 kD protein is found by Western blotting using antibodies specific to galectin-3, exclusively in those cells. Finally, exogenous human recombinant galectin-3 binds strongly to CD34+ early myeloid cells and emphasizes granulocyte-colony stimulating factor (G-CSF) driven proliferation in vitro.
Le Marer
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Academic Surgery Department, ICSTM, 3rd floor, Chelsea and Westminster Hospital, 369 Fulham Rd, London, SW10 9NH, United Kingdom. n.lemarer@ic.ac.uk
D000606:Amino Sugars / Q000378:metabolism; D018952:Antigens, CD34 / Q000032:analysis; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000378:metabolism; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D037502:Galectin 3; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D011134:Polysaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015854:Up-Regulation
D000606:Amino Sugars; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D037502:Galectin 3; D011134:Polysaccharides; D011994:Recombinant Proteins; C066929:polylactosamine