title: Structure of the cytoplasmic domain of p23 in solution: implications for the formation of COPI vesicles.
abstract: Coatomer, the coat protein complex of coat protein (COPI) vesicles, is involved in the budding of these vesicles. Its interaction with the cytoplasmic domains of some p24-family members, type I transmembrane proteins of the Golgi, has been shown to induce a conformational change of coatomer that initiates polymerization of the complex. From stoichiometrical data it is likely that interaction of coatomer with the small tail domains involves an oligomeric form of the p24 proteins. Here we present the structure of peptide analogs of the cytoplasmic domain of p23, a member of the p24 family, as determined by two-dimensional nuclear magnetic resonance spectroscopy in the presence of 2,2,2-trifluoroethanol. An improved strategy for structure calculation revealed that the tail domain peptides form alpha-helices and adopt a tetrameric state. Based on these results we propose an initial model for the binding of coatomer by p23 and the induced conformational change of coatomer that results in its polymerization, curvature of the Golgi membrane to form a bud, and finally a COPI-coated vesicle.
authors.affiliation: Lehrstuhl für Biopolymere, Universität Bayreuth, Germany. mw@ibwf.uni-kl.de
authors.forename: M
authors.initials: M
authors.lastname: Weidler
authors.forename: C
authors.initials: C
authors.lastname: Reinhard
authors.forename: G
authors.initials: G
authors.lastname: Friedrich
authors.forename: F T
authors.initials: FT
authors.lastname: Wieland
authors.forename: P
authors.initials: P
authors.lastname: Rösch
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry*; D020756:Coatomer Protein / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013997:Time Factors; D014270:Trifluoroethanol / Q000378:metabolism
chemical_list: D020756:Coatomer Protein; D010455:Peptides; D014270:Trifluoroethanol