title: Carbohydrate-carbohydrate interaction: zymosan and beta-glucan from Saccharomyces cerevisiae bind mannosylated glycoconjugates.
abstract: Zymosan from cell wall of the yeast Saccharomyces cerevisiae was found to interact with synthetic and natural glycoconjugates; mannose-rich glycoprotein N-chains demonstrated the maximal affinity. The carbohydrate-carbohydrate nature of the interaction has been confirmed by the following data: 1) periodate treatment of the zymosan impairs the binding; 2) neither protease nor alkali treatment of the zymosan weaken the binding; 3) beta-glucan from S. cerevisiae, which is the major zymosan component, interacts with glycoconjugates similarly to zymosan. The binding is reversible, Ca2+-dependent, and cooperative; it can be dose-dependently inhibited by saccharides relative to one of the partners of the carbohydrate-carbohydrate interaction.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117434, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Mikhalchik
authors.forename: S D
authors.initials: SD
authors.lastname: Shiyan
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
mesh_terms: D002118:Calcium / Q000378:metabolism; D002236:Carbohydrate Conformation; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D003101:Collodion / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000378:metabolism; D019793:Fluorescein / Q000378:metabolism; D005936:Glucans / Q000378:metabolism*; D006001:Glycoconjugates / Q000378:metabolism; D020128:Inhibitory Concentration 50; D008358:Mannose / Q000378:metabolism*; D008969:Molecular Sequence Data; D011137:Polystyrenes / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D015054:Zymosan / Q000378:metabolism*
chemical_list: D005936:Glucans; D006001:Glycoconjugates; D011137:Polystyrenes; D003101:Collodion; D015054:Zymosan; D010450:Endopeptidases; D008358:Mannose; D002118:Calcium; D019793:Fluorescein