title: The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines.
abstract: Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor and interleukin-6 (IL-6), which belong to the cytokine receptor family using the common gp130 signal transducer. We studied the actions of two other members of this family, IL-11 and ciliary neurotropic factor (CNTF), on folliculostellate (FS) cells (TtT/GF cell line) and lactosomatotropic cells (GH3 cell line). The messenger RNA (mRNA) for the alpha-chain specific for the IL-11 receptor (1.7 kb) and CNTF receptor (2 kb) are expressed on both cell types. In addition, we detected CNTF receptor mRNA in normal rat anterior pituitary cells. IL-11 (1.25-5 nM) dose dependently stimulated the proliferation of FS cells. CNTF, at doses from 0.4-2 nM, also significantly stimulated the growth of these cells. In addition, both cytokines significantly stimulated proliferation of lactosomatotropic GH3 cells, and CNTF stimulated hormone production (GH and PRL) at 24 h by these cells. At 16-72 h, IL-11 stimulates the secretion of the angiogenic factor vascular endothelial growth factor by FS cells. In addition, both GH3 and FS cells express CNTF mRNA. These data suggest that IL-11 and CNTF may act as growth and regulatory factors in anterior pituitary cells.
authors.affiliation: Department de Biología, FCEN, Universidad de Buenos Aires, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Perez Castro
authors.forename: A C
authors.initials: AC
authors.lastname: Nagashima
authors.forename: M P
authors.initials: MP
authors.lastname: Pereda
authors.forename: V
authors.initials: V
authors.lastname: Goldberg
authors.forename: A
authors.initials: A
authors.lastname: Chervin
authors.forename: P
authors.initials: P
authors.lastname: Largen
authors.forename: U
authors.initials: U
authors.lastname: Renner
authors.forename: G K
authors.initials: GK
authors.lastname: Stalla
authors.forename: E
authors.initials: E
authors.lastname: Arzt
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D020934:Ciliary Neurotrophic Factor / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000378:metabolism; D005260:Female; D017370:Interleukin-11 / Q000502:physiology*; D053703:Interleukin-11 Receptor alpha Subunit; D007774:Lactation / Q000502:physiology*; D008222:Lymphokines / Q000378:metabolism; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020801:Receptor, Ciliary Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000235:genetics; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D020934:Ciliary Neurotrophic Factor; D016228:Endothelial Growth Factors; C508430:Il11ra1 protein, rat; D017370:Interleukin-11; D053703:Interleukin-11 Receptor alpha Subunit; D008222:Lymphokines; D020801:Receptor, Ciliary Neurotrophic Factor; D018123:Receptors, Interleukin; D053702:Receptors, Interleukin-11; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors