Osmomechanical regulation of membrane trafficking in polarized cells.
The regulation of membrane trafficking is thought to be predominantly under the control of agonist-receptor transduction pathways. In the present study, osmomechanical stress due to swelling, a condition often accompanying cell activation, was shown to induce multiple membrane trafficking pathways in polarized absorptive epithelial cells in the absence of agonists. Osmomechanical stress activated rapidly (seconds) pathways of calcium-dependent membrane insertion into the basolateral domain, pathways of calcium-independent membrane retrieval from the basolateral domain, and a novel pathway of transcytosis (transcellular) between basolateral and apical cell domains. These pathways appear to underlie the transfer and regulation of transport proteins amongst cell compartments. This broad affect of osmomechanical stress on trafficking pathways may reflect a global mechanism for redistribution of transport proteins and other membrane components amongst cell compartments during states of mechanical stress.
Reid
J M
JM
Department of Integrative Biology and Pharmacology, The University of Texas-Houston Health Science Center, Houston, Texas, 77030, USA.
O'Neil
R G
RG
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D004705:Endocytosis; D005089:Exocytosis; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D007038:Hypotonic Solutions; D007473:Ion Channels / Q000378:metabolism*; D007546:Isoquinolines / Q000493:pharmacokinetics; D007668:Kidney / Q000378:metabolism; D009994:Osmolar Concentration; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013314:Stress, Mechanical
D005456:Fluorescent Dyes; D007038:Hypotonic Solutions; D007473:Ion Channels; D007546:Isoquinolines; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene; C017475:lucifer yellow; D002118:Calcium