Protective effects of intranasal vaccination with plasmid encoding pseudorabies virus glycoprotein B in mice.
Intranasal administration of plasmid DNA encoding glycoprotein B of pseudorabies virus into mice induced both serum and secretory antibody responses. These mice resisted intranasal challenge with lethal dose of the virus, but did not intraperitoneal challenge. On the other hand, intramuscular injection of the plasmid induced less secretory and higher serum antibody responses than those of intranasally vaccinated mice. None of them was protected from virus challenge. The present results suggest that administration of plasmid DNA encoding glycoprotein B by respiratory mucosal route generates local secretory antibodies which serve to protect animals from pseudorabies virus infection.
Takada
A
A
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Okazaki
K
K
Kida
H
H
D000281:Administration, Intranasal; D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D005260:Female; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids; D011557:Pseudorabies / Q000276:immunology* / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000008:administration & dosage; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage
D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C070915:glycoprotein gII, pseudorabies virus