Apoptotic and non-apoptotic modes of programmed cell death in MCF-7 human breast carcinoma cells.
Apoptosis is a specific mode of programmed cell death (PCD), recognized by characteristic morphological and molecular changes. Here we present evidence for a non-apoptotic type of PCD in human MCF-7 breast carcinoma cells. We used TNF-alpha and tyrphostin AG213 to induce apoptotic and non-apoptotic cell death respectively in vitro. Microscopic and immunohistochemical studies, together with DNA analysis and flow cytometric analysis of p53 and bcl-2 oncogene expression, revealed some novel characteristics of non-apoptotic cell death. We show here for the first time some of the biochemical features of an experimentally induced non-apoptotic PCD and emphasize the distinct biochemical events leading to apoptotic and non-apoptotic PCD.
Amin
F
F
School of Biosciences, Cardiff University, Cardiff, CF1 3TL, UK.
Bowen
I D
ID
Szegedi
Z
Z
Mihalik
R
R
Szende
B
B
D017209:Apoptosis; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002467:Cell Nucleus / Q000648:ultrastructure; D020552:Comet Assay; D003593:Cytoplasm / Q000648:ultrastructure; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D011554:Pseudopodia / Q000648:ultrastructure; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D020032:Tyrphostins / Q000494:pharmacology; D014617:Vacuoles / Q000648:ultrastructure
D004273:DNA, Neoplasm; D019253:Proto-Oncogene Proteins c-bcl-2; D012334:RNA, Neoplasm; D014409:Tumor Necrosis Factor-alpha; D016159:Tumor Suppressor Protein p53; D020032:Tyrphostins; C060152:tyrphostin 47