Inflammatory response after coronary revascularization with or without cardiopulmonary bypass.
BACKGROUND
We sought to investigate the effect of multiple coronary artery bypass grafting (CABG) with or without cardiopulmonary bypass (CPB) on the perioperative inflammatory response.


METHODS
Sixty patients undergoing CABG were randomly assigned to one of two groups: (A) on pump with conventional CPB and cardioplegic arrest, and (B) off pump on the beating heart. Serum samples were collected for estimation of neutrophil elastase, interleukin 8 (IL-8), C3a, and C5a preoperatively and at 1, 4, 12, and 24 hours postoperatively. Furthermore, white blood cell (WBC), neutrophil, and monocyte counts were carried out preoperatively and at 1, 12, 36 and 60 hours postoperatively. Overall incidence of infection and perioperative clinical outcome were also recorded.


RESULTS
The groups were similar in terms of age, weight, gender ratio, extent of coronary disease, left ventricular function, and number of grafts per patient. Neutrophil elastase concentration peaked early after CPB in the on-pump group, with a decline with time. Repeated-measures analysis of variance between groups and comparisons at each time point (modified Bonferroni) showed elastase concentrations were significantly higher in the on-pump than the off-pump group (both p < 0.0001). IL-8 increased significantly after surgery in the on-pump group, with no decline during the observation period (p = 0.01 vs off pump). C3a and C5a rose early after surgery in both groups when compared with baseline values. Postoperative WBC, neutrophil, and monocyte counts were significantly higher in the on-pump than the off-pump group (p < 0.01). Finally, the incidence of postoperative overall infections was significantly higher in the on-pump group (p < 0.0001 vs off pump).


CONCLUSIONS
CABG on the beating heart is associated with a significant reduction in inflammatory response and postoperative infection when compared with conventional revascularization with CPB and cardioplegic arrest.
Ascione
R
R
Bristol Heart Institute, Bristol Royal Infirmary, United Kingdom.
Lloyd
C T
CT
Underwood
M J
MJ
Lotto
A A
AA
Pitsis
A A
AA
Angelini
G D
GD
D000368:Aged; D002315:Cardiopulmonary Bypass; D015926:Complement C3a / Q000032:analysis; D015936:Complement C5a / Q000032:analysis; D001026:Coronary Artery Bypass; D005260:Female; D006324:Heart Arrest, Induced; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood; D019272:Leukocyte Elastase / Q000097:blood; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
D018836:Inflammation Mediators; D016209:Interleukin-8; D015926:Complement C3a; D015936:Complement C5a; D019272:Leukocyte Elastase