Performance of the ImmunoCard STAT! E. coli O157:H7 test for detection of Escherichia coli O157:H7 in stools.
ImmunoCard STAT! E. coli O157:H7 (Meridian Diagnostics, Inc., Cincinnati, Ohio) is a novel rapid (10-min) test for the presence of Escherichia coli O157:H7 in stools. The test may be performed either directly on stool specimens or on an overnight broth culture of stool. In a multicenter prospective study, 14 of 14 specimens positive by culture for E. coli O157:H7 were positive by the ImmunoCard STAT! O157:H7 test, and there were no false positives from 263 culture-negative specimens. In a retrospective study, the test was positive in 339 (81%) of 417 stored culture-positive specimens and the specificity was 95% (98 of 103 specimens). No false positives were associated with alternate stool pathogens. The ImmunoCard STAT! O157:H7 test has high sensitivity and specificity.
Mackenzie
A
A
Child and Youth Clinical Trials Network, Children's Hospital of Eastern Ontario Research Institute, Ottawa, Canada. amackenzie@cyctn.on.ca
Orrbine
E
E
Hyde
L
L
Benoit
M
M
Chan
F
F
Park
C
C
Alverson
J
J
Lembke
A
A
Hoban
D
D
Kennedy
W
W
D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic