title: Seroprevalence of herpes simplex virus type 1 and type 2 in selected German populations-relevance for the incidence of genital herpes.
abstract: This study was carried out to determine the prevalence of antibodies to herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) in selected German populations, such as blood donors, hospital patients, and human immunodeficiency virus (HIV)-seropositive individuals. Serum samples collected between 1996 and 1998 were tested by enzyme immunoassays using monoclonal antibody-selected native gG1 and gG2 as antigens and an immunoblot using type-specific recombinant glycoproteins. Equivocal results were resolved by an "in-house" Western blot assay. The prevalence of HSV-1 antibodies increased steadily with age and reached high levels of >/=88% among subjects 40 years of age or older. In the sample of patients and blood donors, the HSV-2 seroprevalence was 12.8% (95% CI = 11.9-13.8%). About 81% of the HSV-2 seropositive subjects were coinfected with HSV-1. When adjusted for age, there was no difference in the HSV-2 seroprevalence between hospital patients and blood donors. The HSV-2 seroprevalence was significantly higher among women (15%) than among men (10.5%), yielding a female : male odds ratio of 1.5 for hospital patients and of 1.67 for blood donors. Among the HIV-infected population, 91.1% were seropositive for HSV-1 and 47.9% for HSV-2. HIV-infected women have a significantly higher risk of HSV-2 infection than men (odds ratio [OR] = 3.22; 95% confidence ratio [CI] 1.99-5.20). In conclusion, although the rate of infections with HSV-2 is relatively low in the German population, attention should be given to the further development in adolescents, especially in view of a possible decrease of HSV-1 seroprevalence in childhood.
authors.affiliation: Institute for Antiviral Chemotherapy, Friedrich-Schiller University of Jena, Jena, Germany. wutzler@zmkh.ef.uni-jena.de
authors.forename: P
authors.initials: P
authors.lastname: Wutzler
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
authors.forename: I
authors.initials: I
authors.lastname: Färber
authors.forename: U
authors.initials: U
authors.lastname: Eichhorn
authors.forename: B
authors.initials: B
authors.lastname: Helbig
authors.forename: A
authors.initials: A
authors.lastname: Sauerbrei
authors.forename: A
authors.initials: A
authors.lastname: Brandstädt
authors.forename: H F
authors.initials: HF
authors.lastname: Rabenau
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D005260:Female; D005858:Germany / Q000453:epidemiology; D006679:HIV Seropositivity / Q000453:epidemiology / Q000276:immunology; D006558:Herpes Genitalis / Q000097:blood / Q000453:epidemiology*; D006561:Herpes Simplex / Q000097:blood / Q000453:epidemiology*; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D016036:Seroepidemiologic Studies
chemical_list: D000914:Antibodies, Viral