Comparison of assays for anti-endomysial and anti-transglutaminase antibodies for diagnosis of pediatric celiac disease.
BACKGROUND
Anti-endomysial antibodies are sensitive and specific markers for celiac disease. This antibody has recently been identified as an antibody to tissue transglutaminase, an enzyme that cross-links and stabilizes extracellular matrix proteins.


OBJECTIVE
To evaluate the clinical usefulness of an enzyme-linked immunoassay for anti-transglutaminase antibodies, and to compare the results with those of AEA, the current gold standard serological test for celiac disease.


METHODS
Serum samples were collected from 33 patients with biopsy-proven celiac disease and AEA tests were performed. Control samples for anti-transglutaminase were obtained from 155 patients. An ELISA test for immunoglobulin A anti-transglutaminase utilizing guinea pig liver transglutaminase was developed and performed on all sera. Cutoff values for the test were performed using logistic regression and receiver operating curves analysis.


RESULTS
An optical density cutoff value of 0.34 was established for the assay. The mean value was 0.18 +/- 0.19 optical density for controls, and 1.65 +/- 1.14 for patients with celiac disease (P < 0.001). Sensitivity and specificity of the assay were both 90%, while AEA had a sensitivity and specificity of 100% and 94%, respectively.


CONCLUSIONS
A tissue transglutaminase-based ELISA test is both sensitive and specific for detection of celiac disease.
Levine
A
A
Department of Pediatrics, Wolfson Medical Center, Holon, Israel. levinemd@netmedia.net.il
Bujanover
Y
Y
Reif
S
S
Gass
S
S
Vardinon
N
N
Reifen
R
R
Lehmann
D
D
D000704:Analysis of Variance; D001323:Autoantibodies / Q000276:immunology*; D015415:Biomarkers; D016022:Case-Control Studies; D002446:Celiac Disease / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D016015:Logistic Models; D012372:ROC Curve; D012680:Sensitivity and Specificity; D011503:Transglutaminases / Q000276:immunology*
D001323:Autoantibodies; D015415:Biomarkers; D005903:Gliadin; D011503:Transglutaminases