Functional human heme oxygenase has a neuroprotective effect on adult rat ganglion cells after pressure-induced ischemia.
Adult retina subjected to transient ischemia and reperfusion leads to controlled retinal ganglion cell (RGC) death over a period. Modification of intracellular mechanisms through a specific adenoviral vector containing the hemoxygenase gene (HO-1) provides avenues for RGC survival following HO-1 gene transfer and ischemia. RGC death rate was reduced by an average of 15% at 1, 2 and 3 weeks. A significant number of RGC transfected with functional HO-1 survived ischemic insults. Pharmacological stimulation of HO-1 may constitute a novel therapeutic approach to rescuing RGC experiencing ischemic/reperfusion injury.
Hegazy
K A
KA
Department of Ophthalmology, New York Medical College, Valhalla, NY 10595, USA.
Dunn
M W
MW
Sharma
S C
SC
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis; D005822:Genetic Vectors / Q000008:administration & dosage / Q000494:pharmacology; D006419:Heme Oxygenase (Decyclizing) / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics*; D051547:Heme Oxygenase-1; D006801:Humans; D008565:Membrane Proteins; D017628:Microglia / Q000166:cytology / Q000378:metabolism; D008845:Microinjections; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000378:metabolism; D011312:Pressure / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D013266:Stilbamidines; D013477:Superior Colliculi / Q000098:blood supply / Q000187:drug effects; D013997:Time Factors; D014162:Transfection
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D018696:Neuroprotective Agents; D013266:Stilbamidines; C496541:HMOX1 protein, human; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1