title: Deletion analysis of the flagellar switch protein FliG of Salmonella.
abstract: The flagellar motor/switch complex, consisting of the three proteins FliG, FliM, and FliN, plays a central role in bacterial motility and chemotaxis. We have analyzed FliG, using 10-amino-acid deletions throughout the protein and testing the deletion clones for their motility and dominance properties and for interaction of the deletion proteins with the MS ring protein FliF. Only the N-terminal 46 amino acids of FliG (segments 1 to 4) were important for binding to FliF; consistent with this, an N-terminal fragment consisting of residues 1 to 108 bound FliF strongly, whereas a C-terminal fragment consisting of residues 109 to 331 did not bind FliF at all. Deletions in the region from residues 37 to 96 (segments 4 to 9), 297 to 306 (segment 30), and 317 to 326 (segment 32) permitted swarming, though not at wild-type levels; all other deletions caused paralyzed or, more commonly, nonflagellate phenotype. Except for those near the N terminus, deletions had a dominant negative effect on wild-type cells.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-8114, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kihara
authors.forename: G U
authors.initials: GU
authors.lastname: Miller
authors.forename: R M
authors.initials: RM
authors.lastname: Macnab
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002633:Chemotaxis; D004252:DNA Mutational Analysis; D005407:Flagella / Q000235:genetics*; D005816:Genetic Complementation Test; D008565:Membrane Proteins; D020409:Molecular Motor Proteins / Q000235:genetics*; D009068:Movement; D009154:Mutation; D011485:Protein Binding; D012475:Salmonella / Q000235:genetics*; D017384:Sequence Deletion
chemical_list: D001426:Bacterial Proteins; C077389:FliN protein, Bacteria; C075661:Flif protein, Bacteria; C075660:Flig protein, Bacteria; D008565:Membrane Proteins; D020409:Molecular Motor Proteins; C066821:FliM protein, Bacteria