Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria.
A fluorescence-labeled wheat germ agglutinin staining technique (R. K. Sizemore et al., Appl. Environ. Microbiol. 56:2245-2247, 1990) was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.
Fife
D J
DJ
Biotechnology, Idaho National Engineering and Environmental Laboratory, Idaho Falls, Idaho 83415, USA.
Bruhn
D F
DF
Miller
K S
KS
Stoner
D L
DL
D016947:Acetobacteraceae / Q000145:classification / Q000302:isolation & purification*; D020561:Alphaproteobacteria / Q000145:classification / Q000302:isolation & purification*; D007501:Iron; D017879:Isothiocyanates; D037102:Lectins; D008856:Microscopy, Fluorescence / Q000379:methods; D008906:Mining; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity; D012987:Soil; D013440:Sulfides; D016944:Sulfolobus / Q000145:classification / Q000302:isolation & purification; D013855:Thiobacillus / Q000145:classification / Q000302:isolation & purification
D017879:Isothiocyanates; D037102:Lectins; D012336:RNA, Ribosomal, 16S; D012987:Soil; D013440:Sulfides; C011342:pyrite; C037152:isothiocyanic acid; D007501:Iron