Isolation of repeated and self-complementary sequences from E. coli DNA.
We have used the single-strand specific nuclease from Neurospora crassa and chromatography on methylated albumin-kieselguhr to purify and characterize repeated and self-complementary sequences from Escherichia coli DNA. Approximately 0.5% of the genome renatures spontaneously at zero time and another 2% renatures somewhat more rapidly than the total DNA. The early renaturing DNA has a base composition and a Tm similar to the total DNA and contains on the average 100 base pairs; the self-complementary DNA also has a base composition like E. coli but contains a mean of 170 base pairs. No evidence was obtained for the presence of a highly redundant sequence.
Kato
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Borstad
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Fraser
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MJ
Denhardt
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D001482:Base Composition; D002853:Chromatography, Liquid; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D004277:DNA, Single-Stranded / Q000378:metabolism; D007692:Diatomaceous Earth; D004706:Endodeoxyribonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism; D009492:Neurospora crassa / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012710:Serum Albumin, Bovine
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D005656:Fungal Proteins; C029180:methylated bovine serum albumin; D012710:Serum Albumin, Bovine; D007692:Diatomaceous Earth; D004706:Endodeoxyribonucleases