Evaluation of the prothrombin gene polymorphism in patients with advanced retinopathy of prematurity.
It has been reported recently that a common genetic variant in the 3'-untranslated region of the prothrombin gene is associated with a significant fraction of premature births. The purpose of this study is to evaluate the prothrombin gene polymorphism in a large cohort of patients with preterm birth and advanced retinopathy of prematurity. For this purpose, the leukocyte DNAs were analyzed for the mutation (20210A) in the 3'-untranslated region of the prothrombin gene by PCR amplification, followed by restriction analysis and DNA sequencing. Our extensive analysis revealed a normal genotype (GG) in all patients as well as controls. These results suggest that the common genetic variant in the 3'-untranslated region of the prothrombin gene is not associated with advanced retinopathy of prematurity. Although more patients' samples should be evaluated, this genetic test does not support a relationship between prothrombin gene mutation and retinopathy of prematurity.
Hiraoka
M
M
Eye Research Institute, Oakland University, Rochester, MI 48309, USA.
Shastry
B S
BS
D020413:3' Untranslated Regions / Q000235:genetics*; D000483:Alleles; D044383:Black People / Q000235:genetics; D015331:Cohort Studies; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004252:DNA Mutational Analysis; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007962:Leukocytes / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests; D011516:Prothrombin / Q000235:genetics*; D012178:Retinopathy of Prematurity / Q000235:genetics*; D044465:White People / Q000235:genetics
D020413:3' Untranslated Regions; D011516:Prothrombin; D004247:DNA