Generation of electrospray from a solution predeposited on optical fibers coiled with a platinum wire.
This study examines the feasibility of generating electrospray directly from the tip of two optical fibers bound together with Teflon tape. This approach does not require a capillary and syringe pump. The electrospray source is simply constructed by coiling the two optical fibers with a platinum (Pt) wire. The optical fibers extend beyond the Pt coil for approximately 1 cm. The sample solution is predeposited on the Pt coil by a micropipette. As the high voltage required for electrospray is applied to the coil, the sample solution moves along the grooves between the two optical fibers. A stable electrospray is subsequently generated at the tip of the fibers. The mass spectra of insulin, lysozyme, and ubiquitin are exactly the same as those obtained by conventional electrospray using a capillary and syringe pump. Rapid determination of the active ingredient in a tablet by this technique is demonstrated.
Kuo
C P
CP
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Yuan
C H
CH
Shiea
J
J
D005336:Fiber Optic Technology; D007328:Insulin / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D009113:Muramidase / Q000032:analysis; D055100:Optical Fibers; D010726:Phosphodiesterase Inhibitors / Q000032:analysis; D010879:Piperazines / Q000032:analysis; D010984:Platinum; D011687:Purines; D000068677:Sildenafil Citrate; D013450:Sulfones; D013607:Tablets; D014452:Ubiquitins / Q000032:analysis
D007328:Insulin; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D013607:Tablets; D014452:Ubiquitins; D010984:Platinum; D000068677:Sildenafil Citrate; D009113:Muramidase