Transfer RNA methylating activity of yeast mitochondria.
Mitochondria isolated from Saccharomyces cerevisiae and purified in Urografin or sucrose gradient contain tRNA methylating activity with specificities different from those of the cytoplasm. The main reaction product, using E.coli tRNA as methyl group acceptor, is N(2),-N(2)-dimethylguanine. The corresponding mitochondrial methylase is coded by nuclear DNA. A DNA methylating activity is also associated with yeast mitochondria.
Smolar
N
N
Svensson
I
I
D004926:Escherichia coli / Q000378:metabolism; D008745:Methylation; D008928:Mitochondria / Q000378:metabolism*; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012359:tRNA Methyltransferases / Q000378:metabolism
D012329:RNA, Bacterial; D012343:RNA, Transfer; D012359:tRNA Methyltransferases