Evaluation of scanners and CCD cameras for high-resolution TEM of protein crystals and single particles.
The modulation transfer function (MTF) and the geometric errors of two flatbed scanners, a slow-scan CCD (SSC) camera and film, have been measured and compared. The geometric errors of the SSC camera and film have been measured using diffraction spots from a lipid crystal. The SSC camera was shown to have the smallest geometric errors while film had the best MTF. Even though film had the best MTF, this is significantly reduced when scanning the film, so that the MTF of the film and scanner combined are comparable to the MTF of the SSC camera.
Hesse
J
J
Karolinska Institute, Department of Bioscience at Novum, S-14157 Huddinge, Sweden.
Hebert
H
H
Koeck
P J
PJ
D055592:Biophysical Phenomena; D001703:Biophysics; D003460:Crystallization; D008854:Microscopy, Electron / Q000295:instrumentation* / Q000379:methods; D008962:Models, Theoretical; D011506:Proteins / Q000648:ultrastructure
D011506:Proteins