title: [Initiation of lipid peroxidation (LPO) in blood during intraoperative mechanical autotransfusion--is hepatotoxicity of lipid peroxidation products of clinical significance?].
abstract: Machine autotransfusion using cell-saver is a well-established method of saving homologous blood during extensive surgical procedures. The processing of blood may induce the initiation of lipid peroxidation (LPO) with the release of hepatotoxic products. A series of 42 patients undergoing primary (n = 20) or revision (n = 22) hip arthroplasty comprised the study group. Patients received an average of 1,260 ml of autologous blood and 2.2 units of homologous packed cells. The concentration of thiobarbituric acid reactive substances (TBARS) as LPO metabolites was measured in the patients' plasma, in the autologous packed cells as well as in the supernatants of the cell-saver-processed blood. Additionally, parameters of iron metabolism, haemoglobin levels, haematocrit as well as the activities of so-called liver enzymes aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidase and cholinesterase were determined. An initiation of LPO was detectable during the process of machine autotransfusion, but this took place mainly ex vivo. High concentrations of TBARS were detectable in the supernatants after cell-separation processing. We observed a decline in haemoglobin concentration and haematocrit during the perioperative period. Postoperatively, we found a significant iron deficiency as a consequence of the perioperative blood loss. There was not sufficient evidence of a postoperative liver disorder induced by toxic metabolites of LPO. To sum up, there is only a low contamination of the organism with LPO products during the process of machine autotransfusion. Therefore, an induction of liver damage seems to be improbable.
authors.affiliation: Klinik für Anästhesiologie und Intensivtherapie, Waldklinikums Gera gGmbH (Akademisches Lehrkrankenhaus der Friedrich-Schiller-Universität Jena).
authors.forename: M
authors.initials: M
authors.lastname: Kretzschmar
authors.forename: M
authors.initials: M
authors.lastname: Münster
authors.forename: W
authors.initials: W
authors.lastname: Schirrmeister
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019644:Arthroplasty, Replacement, Hip; D001804:Blood Transfusion, Autologous; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008099:Liver / Q000503:physiopathology; D008107:Liver Diseases / Q000209:etiology* / Q000503:physiopathology; D008111:Liver Function Tests; D008297:Male; D008875:Middle Aged; D012086:Reoperation; D012307:Risk Factors; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
chemical_list: D017392:Thiobarbituric Acid Reactive Substances