title: Cloning and Expression of an Elongation Factor-1alpha in Sea Bream (Sparus aurata) Larvae and Adult Tissue.
abstract: A clone encoding the polypeptide elongation factor EF-1alpha was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1alpha in other animal species. EF-1alpha messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1alpha appeared to have a relatively uniform distribution across all the tissues analyzed.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Museum Avenue, P.O. Box 911, Cardiff CF1 3US, Wales, U.K.
authors.initials: MA
authors.lastname: Nowell
authors.initials: DM
authors.lastname: Power
authors.initials: PM
authors.lastname: Guerreiro
authors.initials: L
authors.lastname: Llewellyn
authors.initials: V
authors.lastname: Ramsurn
authors.initials: T
authors.lastname: Wigham
authors.initials: GE
authors.lastname: Sweeney