Translation termination factor aRF1 from the archaeon Methanococcus jannaschii is active with eukaryotic ribosomes.
Class-1 translation termination factors (release factors (RFs)) from Eukarya (eRF1) and Archaea (aRF1) exhibit a high degree of amino acid sequence homology and share many common motifs. In contrast to eRF1, function(s) of aRF1 have not yet been studied in vitro. Here, we describe for the first time the cloning and expression in Escherichia coli of the gene encoding the peptide chain RF from the hyperthermophilic archaeon Methanococcus jannaschii (MjaRF1). In an in vitro assay with mammalian ribosomes, MjaRF1, which was overproduced in E. coli, was active as a RF with all three termination codon-containing tetraplets, demonstrating the functional resemblance of aRF1 and eRF1. This observation confirms the earlier prediction that eRF1 and aRF1 form a common structural-functional eRF1/aRF1 protein family, originating from a common ancient ancestor.
Dontsova
M
M
Institute of Protein Research, Russian Academy of Sciences, 142292, Pushchino, Russia.
Frolova
L
L
Vassilieva
J
J
Piendl
W
W
Kisselev
L
L
Garber
M
M
D000595:Amino Acid Sequence; D000818:Animals; D019843:Archaeal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018387:Codon, Initiator; D005057:Eukaryotic Cells / Q000378:metabolism; D006801:Humans; D017017:Methanococcus / Q000235:genetics; D008969:Molecular Sequence Data; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D012270:Ribosomes / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D019843:Archaeal Proteins; D018387:Codon, Initiator; C489002:ETF1 protein, human; D010454:Peptide Termination Factors