title: Metabolism of methanol in plant cells. Carbon-13 nuclear magnetic resonance studies.
abstract: Using (13)C-NMR, we demonstrate that [(13)C]methanol readily entered sycamore (Acer pseudoplatanus L.) cells to be slowly metabolized to [3-(13)C]serine, [(13)CH(3)]methionine, and [(13)CH(3)]phosphatidylcholine. We conclude that the assimilation of [(13)C]methanol occurs through the formation of (13)CH(3)H(4)Pte-glutamate (Glu)(n) and S-adenosyl-methionine, because feeding plant cells with [3-(13)CH(3)]serine, the direct precursor of (13)CH(2)H(4)Pte-Glu(n), can perfectly mimic [(13)CH(3)]methanol for folate-mediated single-carbon metabolism. On the other hand, the metabolism of [(13)C]methanol in plant cells revealed assimilation of label into a new cellular product that was identified as [(13)CH(3)]methyl-beta-D-glucopyranoside. The de novo synthesis of methyl-beta-D-glucopyranoside induced by methanol did not require the formation of (13)CH(3)H(4)Pte-Glu(n) and was very likely catalyzed by a "transglycosylation" process.
authors.affiliation: Laboratoire de Résonance Magnétique en Biologie Métabolique, Département de Biologie Moléculaire et Structurale, CEA-38054, Grenoble cedex 9, France.
authors.forename: E
authors.initials: E
authors.lastname: Gout
authors.forename: S
authors.initials: S
authors.lastname: Aubert
authors.forename: R
authors.initials: R
authors.lastname: Bligny
authors.forename: F
authors.initials: F
authors.lastname: Rébeillé
authors.forename: A R
authors.initials: AR
authors.lastname: Nonomura
authors.forename: A A
authors.initials: AA
authors.lastname: Benson
authors.forename: R
authors.initials: R
authors.lastname: Douce
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D000432:Methanol