Temperature dependence of fusion by sendai virus.
Studies of the temperature dependence of liposome fusion by Sendai virus indicate that fusion occurs maximally at 55 degrees C. The fusion capacity of the virus is also inactivated maximally by preincubation at this temperature and, under the same conditions, the F glycoprotein becomes resistant to proteolysis. By analogy with the activation at elevated temperatures of fusion by influenza virus our results suggest that temperature is also a variable in the activation of fusion by paramyxoviruses and possibly in the activation of other members of the group of viruses that includes myxo-, paramyxo-, retro-, and filoviruses, which all contain cleaved, trimeric fusion glycoproteins.
Wharton
S A
SA
Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom.
Skehel
J J
JJ
Wiley
D C
DC
D000818:Animals; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism; D008081:Liposomes; D008561:Membrane Fusion; D018108:Respirovirus / Q000502:physiology*; D013696:Temperature; D014760:Viral Fusion Proteins / Q000378:metabolism
D006023:Glycoproteins; D008081:Liposomes; D014760:Viral Fusion Proteins