Preparation of fibrin des-AA by thrombin.
The active thrombin is formed in the blood stream when the blood coagulation system is activated. It attacks fibrinogen, splits off two fibrinopeptides A and fibrinogen is transformed into des-AA fibrin which is able to polymerize spontaneously forming protofibrils. At high thrombin concentration the enzyme splits off two fibrinopeptides B and des-AA fibrin units are transformed into des-AABB fibrin. These two forms of fibrin are widely used in the biological experiments. However des-AA fibrin is obtained usually from fibrinogen using the snake poisons (such as reptilase). Des-AA fibrin was obtained also by physiological enzyme thrombin, but that des-AA fibrin samples had the contamination of des-AABB fibrin. At the present paper we have described the method of the des-AA fibrin preparation by thrombin without any contamination of des-AABB fibrin.
Lougovskoi
E V
EV
A.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Gogolinskaya
G K
GK
D001704:Biopolymers; D005337:Fibrin / Q000737:chemistry*; D010446:Peptide Fragments / Q000138:chemical synthesis*; D013917:Thrombin / Q000737:chemistry*
D001704:Biopolymers; D010446:Peptide Fragments; D005337:Fibrin; D013917:Thrombin