A hyperglycemic peptide hormone from the Caribbean shrimp Penaeus (litopenaeus) schmitti.
From a crude extract of the sinus glands of the shrimp Penaeus (litopenaeus) schmitti a peptide with hyperglycemic activity in a homologous bioassay was isolated and characterized by a combination of automatic Edman degradation, enzymatic digestions, TLC of dansyl-amino acids, and mass spectrometry. Its M(r) is 8359.4 Da by MS, which coincides with the deduced sequence. Its N-terminus is free and its C-terminus is amidated. It has 6 Cys residues in conserved positions compared with other known CHHs. This is the first sinus gland hormone from an Atlantic Ocean shrimp characterized to date. It has a remarkable 90% sequence similarity to the Indo-Pacific shrimp P. (marsupenaeus) japonicus Pej-VII hyperglycemic hormone.
Huberman
A
A
Instituto Nacional de la Nutrición 'Salvador Zubirán,' Department of Biochemistry, Vasco de Quiroga 15, Tlalpan, Mexico City, Mexico. amhuber@servidor.unam.mx
Aguilar
M B
MB
Navarro-Quiroga
I
I
Ramos
L
L
Fernández
I
I
White
F M
FM
Hunt
D F
DF
Shabanowitz
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D004702:Endocrine Glands / Q000737:chemistry*; D010450:Endopeptidases; D005947:Glucose / Q000378:metabolism*; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033561:Penaeidae; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D007447:Invertebrate Hormones; D010450:Endopeptidases; D005947:Glucose