The structure and properties of histone F2a comprising the heterologous group F2a1 and F2a2 studied by 13C nuclear magnetic resonance.
(13)C n.m.r. spectra have been obtained for aqueous solutions of histones F2a(1) and F2a(2), for the group F2a, for the appropriate amino acid mixturesand for the corresponding hydrolysates. These, when compared with computer simulated spectra give good agreement for secondary structure with that calculated from the known primary structure of the proteins. Evidence based on the spectra obtained at various salt concentrations leads to the conclusion that F2a is not a simple mixture but an interacting heterologous group of histones F2a(1) and F2a(2).
Clark
V M
VM
Lilley
D M
DM
Howarth
O W
OW
Richards
B M
BM
Pardon
J F
JF
D000818:Animals; D002247:Carbon Isotopes; D002417:Cattle; D003198:Computer Simulation; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D017433:Protein Structure, Secondary; D012996:Solutions; D014867:Water
D002247:Carbon Isotopes; D006657:Histones; D012996:Solutions; D014867:Water