Rat histidine decarboxylase is a substrate for m-calpain in vitro.
We have followed the in vitro degradation of rat histidine decarboxylase in a reconstituted system, containing only rat histidine decarboxylase (obtained by in vitro transcription and translation), calcium ions in the millimolar range of concentrations, and m-calpain. Under the experimental conditions used, m-calpain quickly and efficiently degraded rat histidine decarboxylase, giving rise to a major proteolytic band of 29 kDa. In a conventional in vitro degradation system containing rabbit reticulocytes supplemented with calcium ions, there was also an intense proteolysis of rat histidine decarboxylase, strongly inhibited in the presence of calpeptin, a highly specific calpain inhibitor.
Rodríguez-Agudo
D
D
Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, Málaga, E-29071, Spain.
Olmo
M T
MT
Sánchez-Jiménez
F
F
Medina
M A
MA
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002154:Calpain / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004151:Dipeptides / Q000494:pharmacology; D006640:Histidine Decarboxylase / Q000378:metabolism*; D007700:Kinetics; D010446:Peptide Fragments / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism; D013379:Substrate Specificity
D015853:Cysteine Proteinase Inhibitors; D004151:Dipeptides; D010446:Peptide Fragments; D011994:Recombinant Proteins; C071482:calpeptin; D002154:Calpain; D006640:Histidine Decarboxylase; D002118:Calcium