Heterologous mischarging as a means of tRNA fractionation. I. Behaviour of E. coli phenylalanyl-tRNA(1Val) on BD-cellulose.
The chromatographic behaviour of E.coli tRNA(Val) (1), Val-tRNA(Val) (1) and Phe-tRNA(Val) (1) was studied on BD-cellulose columns. At pH 4.0 and 4 degrees C the elution position of Phe-tRNA(Val) (1) was not affected by the presence of absence of Mg(2+), whereas Val-tRNA(Val) (1) was slightly retarded when Mg(2+) was ommited. It is postulated that the amino acid and its nature influence the structure that the aminoacyl-tRNA assumes. Under suitable conditions the heteroaminoacylated Phe-tRNA(Val) (1) eluted significantly later than other tRNAs. This fact showed that heterologous mischarging can be a useful step in tRNA purification methods.
Rosenmann
E
E
Krauskopf
M
M
D002848:Chromatography, DEAE-Cellulose; D003636:DEAE-Cellulose / Q000031:analogs & derivatives; D004926:Escherichia coli / Q000737:chemistry; D008274:Magnesium; D009492:Neurospora crassa / Q000201:enzymology; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism; D012329:RNA, Bacterial / Q000302:isolation & purification* / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification / Q000378:metabolism; D012366:RNA, Transfer, Val / Q000302:isolation & purification* / Q000378:metabolism
D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012366:RNA, Transfer, Val; C055068:benzoylated DEAE-cellulose; D003636:DEAE-Cellulose; D010652:Phenylalanine-tRNA Ligase; D008274:Magnesium