Flow cytometric DNA analyses of epithelial dysplasia of the esophagus.
OBJECTIVE
To investigate, with flow cytometry, DNA aneuploidy as a marker of early carcinogenesis in dysplastic esophageal lesions.


METHODS
DNA content of exfoliated cells from 789 cases of esophageal dysplasia (including mild dysplasia, 195 cases; moderate dysplasia, 383 cases; and severe dysplasia, 211 cases) was determined with a FACS 420 flow cytometer.


RESULTS
Cellular DNA content was closely related to the severity of dysplasia. The carcinogenesis rate in patients with dysplasia showed that DNA aneuploidy was significantly higher than in patients showing DNA diploidy.


CONCLUSIONS
DNA aneuploidy in dysplastic lesions is a very important early signal of carcinogenesis. Patients with dysplastic lesions showing DNA aneuploidy should be treated and closely followed.
Zuo
L
L
Department of Cellular Analysis, Hebei Tumor Institute, Shi Jiazhuang, People's Republic of China.
Ling
P
P
Qi
F
F
Wang
J
J
Ding
Z
Z
Guo
J
J
Liu
J
J
D000782:Aneuploidy; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D011003:Ploidies; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology*; D011237:Predictive Value of Tests
D004273:DNA, Neoplasm; D004247:DNA