Determination of the immunodominant part in the O-antigenic polysaccharide from Escherichia coli O128 by ELISA-inhibition study.
The immunodominant part in the O-antigenic polysaccharide from Escherichia coli O128 was immunologically characterized by an enzyme-linked immunosorbent assay (ELISA). The antibody specificity was determined by the inhibitory effects of the methyl glycosides of constituent mono- and oligosaccharides synthesized related to the O-antigenic polysaccharide from E. coli O128. It was found that methyl alpha-L-fucopyranoside was the most effective inhibitor amongst the monosaccharides while the highest antibody specificity was directed towards the trisaccharide with the structure: beta-D-GalpNAc-(1-->6)-[alpha-L-Fucp-(1-->2)]-beta-D-Galp-1-->OMe suggesting that the monospecific antibody has the extended combining site.
Sengupta
P
P
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta, India.
Bhattacharyya
T
T
Majumder
M
M
Chatterjee
B P
BP
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000937:Antigen-Antibody Reactions; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D006027:Glycosides / Q000494:pharmacology; D006801:Humans; D016056:Immunodominant Epitopes / Q000032:analysis*; D007074:Immunoglobulin G / Q000276:immunology; D007231:Infant, Newborn; D009005:Monosaccharides / Q000494:pharmacology; D019081:O Antigens / Q000276:immunology*; D009844:Oligosaccharides / Q000494:pharmacology; D011817:Rabbits
D000907:Antibodies, Bacterial; D006027:Glycosides; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D009005:Monosaccharides; D019081:O Antigens; D009844:Oligosaccharides