title: Accurate mass measurement of low molecular weight compounds by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: We report the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the accurate measurement of mass of low molecular weight compounds (smaller than 1500 Da), a linear peptide, two types of cyclic depsipeptides, a polyhydroxy-macrocyclic lactone, and two prenylated flavonoids, with delayed extraction in the reflector mode. The performance of the MALDI-TOF instrument was less than those of fast atom bombardment and Fourier-transform ion cyclotron resonance mass spectrometry instruments and insufficient to give acceptable accuracy for literature reporting. Nevertheless, when combined with NMR spectrometry and/or amino acid analysis to give information on the numbers of carbon atoms and index of hydrogen deficiency, MALDI was useful for determination of the elemental composition of the low molecular weight compounds available in small quantities.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Chiba, Japan. fukai@phar.toho-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Fukai
authors.forename: J
authors.initials: J
authors.lastname: Kuroda
authors.forename: T
authors.initials: T
authors.lastname: Nomura
mesh_terms: D000804:Angiotensin II / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis; D000935:Antifungal Agents / Q000032:analysis; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis; D001426:Bacterial Proteins; D047630:Depsipeptides; D004365:Drugs, Chinese Herbal / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D007783:Lactones / Q000032:analysis; D008970:Molecular Weight; D009842:Oligopeptides / Q000032:analysis; D010456:Peptides, Cyclic / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D001426:Bacterial Proteins; C096939:Bu 2841-10; D047630:Depsipeptides; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D007783:Lactones; D009842:Oligopeptides; D010456:Peptides, Cyclic; C106437:fusaricidin C; C106438:fusaricidin D; C000507:copiamycin; D000804:Angiotensin II; C057451:morusin; C095195:kuwanon H