Quantitative radioluminography of (125)Iodine whole-body autoradiograms.
Radioluminography (RLG) was applied for the quantitation of (125)iodine ((125)I) autoradiograms using the bioimaging analyzer BAS 2000. RLG was performed on 50-microm sections of sagittal whole-body sections of rats and (125)I-radiolabeled erythrocyte calibration scales. Linearity, sensitivity, accuracy, and reproducibility were investigated in comparison to direct (125)I radioactivity measurement. RLG is demonstrated to be a simple, reproducible, and precise analytical tool. However, the spatial resolution of quantitative (125)I RLG is limited by the relatively high average free path of gamma-radiation and X rays.
Günther
C
C
Research Laboratories, Schering AG, Berlin, D-13342, Germany.
Panzer
M
M
Täuber
U
U
D000818:Animals; D002138:Calibration; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics*; D008163:Luminescent Measurements; D011874:Radiometry / Q000592:standards / Q000706:statistics & numerical data*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014018:Tissue Distribution; D014915:Whole-Body Counting / Q000592:standards / Q000706:statistics & numerical data*
D007457:Iodine Radioisotopes