An exfoliative toxin A-converting phage isolated from Staphylococcus aureus strain ZM.
Exfoliative toxin A (ETA) causes staphylococcal scalded-skin syndrome in children. The gene for ETA was believed to be coded by the chromosomal DNA. We isolated temperate phages from an ETA-producing strain, ZM, using a restriction minus strain, 1039, as an indicator. One of the prophages, designated phi-ZM-1 mediated lysogenic conversion of ETA. The polymerase chain reaction assay of the eta gene revealed that phage phi-ZM-1 carries the structural gene for ETA.
Yoshizawa
Y
Y
Radioisotope Research Center, The Jikei University School of Medicine, Tokyo, Japan. yukio.yoshizawa@nifty.ne.jp
Sakurada
J
J
Sakurai
S
S
Machida
K
K
Kondo
I
I
Masuda
S
S
D005083:Exfoliatins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008242:Lysogeny; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction / Q000379:methods; D013206:Staphylococcal Scalded Skin Syndrome / Q000382:microbiology*; D013204:Staphylococcus Phages / Q000235:genetics* / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000145:classification / Q000378:metabolism / Q000821:virology*
D005083:Exfoliatins