title: Differentiation of Aedes triseriatus (Say) from Aedes hendersoni Cockerell (Diptera: Culicidae) by restriction fragment length polymorphisms of amplified ribosomal DNA.
abstract: Aedes triseriatus is the primary vector of LaCrosse (LAC) virus, which can cause encephalitis, especially in young children. Aedes hendersoni, a sibling species of Ae. triseriatus, has a salivary gland barrier to LAC virus and, therefore, is not considered a vector of this virus. Adults of Ae. triseriatus are morphologically indistinguishable from those of Ae. hendersoni, and the two species are sympatric in the eastern United States. A definitive method of identifying field specimens is an important part of any disease surveillance program, particularly in the case of LAC virus. This study identifies restriction enzymes that produce species-specific restriction fragment length polymorphisms (RFLPs) from amplified ribosomal (r) DNA. In addition, sequences of the internal transcribed spacers 1 and 2 and the 5.8S regions of the rDNA were used to confirm the RFLP patterns. This study is the first to compare nucleotide sequences from Ae. triseriatus and Ae. hendersoni.
authors.affiliation: Medical Entomology Program, Illinois Natural History Survey, Champaign 61820, USA.
authors.forename: H E
authors.initials: HE
authors.lastname: Reno
authors.forename: M H
authors.initials: MH
authors.lastname: Vodkin
authors.forename: R J
authors.initials: RJ
authors.lastname: Novak
mesh_terms: D000330:Aedes / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D018792:Encephalitis, Viral / Q000635:transmission* / Q000821:virology; D007087:Illinois; D007303:Insect Vectors / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal