Wound-induced expression of a tobacco peroxidase is not enhanced by ethephon and suppressed by methyl jasmonate and coronatine.
In tobacco plants, wounding induces production of a set of defense-related proteins such as basic pathogenesis-related (PR) proteins and proteinase inhibitors (PIs) via the jasmonate/ethylene pathway. Although class III plant peroxidase (POX) is also wound-inducible, the regulatory mechanism for its wound-induced expression is not fully understood. Here, we describe that a tobacco POX gene (tpoxN1), which is constitutively expressed in roots, is induced locally 30 min after wounding and then systemically in tobacco plants. Infection of necrotizing virus also induced tpoxN1 gene. The wound-induced expression was not enhanced by known wound-signal compounds such as methyl jasmonate (MeJA) and ethephon in contrast to other wound-inducible genes such as basic PR-1 and PI-II genes. And treatment with MeJA and coronatine, biological analogs of jasmonate, rather suppressed the tpoxN1 expression. Salicylic acid, an antagonist of jasmonate-based wound signaling, did not suppress the wound-induced expression of tpoxN1. Only spermine, which is reported as an endogenous inducer for acidic PR genes in tobacco mosaic virus-infected tobacco leaves, could induce tpoxN1 gene expression. These results suggest that wound-induced expression of the tpoxN1 gene is regulated differently from that of the basic PR and PI-II genes.
Hiraga
S
S
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Ito
H
H
Sasaki
K
K
Yamakawa
H
H
Mitsuhara
I
I
Toshima
H
H
Matsui
H
H
Honma
M
M
Ohashi
Y
Y
D000085:Acetates / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007192:Indenes / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D054883:Oxylipins; D009195:Peroxidase / Q000096:biosynthesis* / Q000235:genetics; D010937:Plant Growth Regulators / Q000378:metabolism*; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000502:physiology
D000085:Acetates; D000596:Amino Acids; D003517:Cyclopentanes; D007192:Indenes; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010937:Plant Growth Regulators; C058487:coronatine; C072239:methyl jasmonate; D009195:Peroxidase; C005073:ethephon