Familial Mediterranean fever: effects of genotype and ethnicity on inflammatory attacks and amyloidosis.
OBJECTIVE
The gene causing familial Mediterranean fever (FMF)-an autosomal recessive disease characterized by recurrent short episodes of fever associated most commonly with peritonitis, pleuritis, and arthritis-has recently been found and several mutations identified. The most severe complication of the disease is amyloidosis, which can lead to renal failure. The aim of this study was to investigate the role of genetic versus nongenetic factors on the phenotype as well as on the development of amyloidosis in FMF in a large and heterogeneous group of patients.


METHODS
We studied 382 patients from 4 ethnic origins living in different environments: North African Jews, other Jews, Turks, Armenians living in the United States, and Armenians from Yerevan, Armenia. Information regarding amyloidosis was available for 371 patients. We examined the association between the mutation M694V and the development of amyloidosis, and we also compared the clinical characteristics of the inflammatory attacks in patients from different ethnic origins, while controlling for the type of mutation.


RESULTS
A significant association was found between amyloidosis and the most common mutation in exon 10 of the FMF gene (MEFV), M694V (for M694V homozygotes, relative risk = 1.77; 95% CI = 1.16-2.71). Amyloidosis was present in 44 of 171 homozygous FMF patients (25.7%), in 22 of 143 compound heterozygous FMF patients (15.4%), and in 7 of 57 patients carrying other mutations (12.3%). In homozygotes for M694V who had not been treated with colchicine before 20 years of age, the risk of amyloidosis developing before this age was 61.0%. In our series, there were no cases of amyloidosis in 16 patients carrying the common mutation E148Q. We found that the type and severity of the FMF inflammatory symptoms were associated with both the genotype and the country of residence of the patient.


CONCLUSIONS
In the light of the high frequency of amyloidosis in homozygotes for the mutation M694V, colchicine treatment should be given to this group irrespective of the severity of the inflammatory attacks to prevent the development of amyloidosis. Our findings also suggest that factors other than genotype, such as environment or genes other than MEFV, play a role in the determination of the severity of the inflammatory attacks in FMF. amyloidosis, specific mutation, phenotype-genotype correlation, ethnicity.
Mimouni
A
A
Department of Medical Genetics, FMRC and Beilinson Campus, Rabin Medical Center and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
Magal
N
N
Stoffman
N
N
Shohat
T
T
Minasian
A
A
Krasnov
M
M
Halpern
G J
GJ
Rotter
J I
JI
Fischel-Ghodsian
N
N
Danon
Y L
YL
Shohat
M
M
D017668:Age of Onset; D000686:Amyloidosis / Q000209:etiology* / Q000517:prevention & control; D003078:Colchicine / Q000627:therapeutic use; D004777:Environment; D010505:Familial Mediterranean Fever / Q000150:complications / Q000188:drug therapy / Q000208:ethnology* / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007249:Inflammation / Q000209:etiology; D009154:Mutation; D012111:Residence Characteristics
D003078:Colchicine