Arginine kinase from Nautilus pompilius, a living fossil. Site-directed mutagenesis studies on the role of amino acid residues in the Guanidino specificity region.
Arginine kinases were isolated from the cephalopods Nautilus pompilius, Octopus vulgaris, and Sepioteuthis lessoniana, and the cDNA-derived amino acid sequences have been determined. Although the origin and evolution of cephalopods have long been obscure, this work provides the first molecular evidence for the phylogenetic position of Cephalopoda in molluscan evolution. A crystal structure for Limulus arginine kinase showed that four amino acid residues (Ser(63), Gly(64), Val(65), and Tyr(68)) are hydrogen-bonded with the substrate arginine. We introduced three independent mutations, Ser(63) --> Gly, Ser(63) --> Thr, and Tyr(68) --> Ser, in Nautilus arginine kinase. One of the mutants had a considerably reduced substrate affinity, accompanied by a decreased V(max). In other mutants, the activity was lost almost completely. It is known that substantial conformational changes take place upon substrate binding in arginine kinase. We hypothesize that the hydrogen bond between Asp(62) and Arg(193) stabilizes the closed, substrate-bound state. Site-directed mutagenesis studies strongly support this hypothesis. The mutant (Asp(62) --> Gly or Arg(193) --> Gly), which destabilizes the maintenance of the closed state and/or perhaps disrupts the unique topology of the catalytic pocket, showed only a very weak activity (0.6-1.5% to the wild-type).
Suzuki
T
T
Laboratory of Biochemistry, Faculty of Science, Kochi University, Kochi 780-8520, Japan. suzuki@sc.kochi-u.ac.jp
Fukuta
H
H
Nagato
H
H
Umekawa
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001122:Arginine Kinase / Q000235:genetics*; D001483:Base Sequence; D020134:Catalytic Domain / Q000235:genetics; D049832:Decapodiformes / Q000145:classification / Q000201:enzymology / Q000235:genetics; D019143:Evolution, Molecular; D005998:Glycine / Q000235:genetics; D006737:Horseshoe Crabs / Q000145:classification / Q000201:enzymology / Q000235:genetics; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008974:Mollusca / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D049831:Octopodiformes / Q000145:classification / Q000201:enzymology / Q000235:genetics; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000235:genetics; D014443:Tyrosine / Q000235:genetics; D014633:Valine / Q000235:genetics
D014443:Tyrosine; D012694:Serine; D001122:Arginine Kinase; D014633:Valine; D005998:Glycine