Evidence of involvement of the PLAG1 gene in lipoblastomas.
Previous cytogenetic studies have demonstrated that the majority of lipoblastomas show rearrangements, in particular translocations and insertions, with breakpoints in 8q11-13. Here we present evidence for involvement of the developmentally regulated zink finger gene PLAG1 in these rearrangements. Northern blot and RT-PCR analyses revealed overexpression of PLAG1 in two lipoblastomas. Using immunohistochemistry, expression of the PLAG1 protein was also demonstrated in tissue sections from two lipoblastomas, one of which had a t(3;8)(q13.1;q12) translocation and the other a t(1;6)(q42;p22) translocation. Since no aberrant PLAG1 transcripts could be detected, it is likely that the gene may be activated by promoter swapping/substitution or alternatively by an as yet unknown mechanism. Our findings indicate that PLAG1 activation is a recurrent event in lipoblastomas and that PLAG1 is likely to be the target gene on chromosome 8 in these tumors.
Astrom
A
A
Lundberg Laboratory for Cancer Research, Department of Pathology, Goteborg University, Goteborg, Sweden.
D'Amore
E S
ES
Sainati
L
L
Panarello
C
C
Morerio
C
C
Mark
J
J
Stenman
G
G
D002460:Cell Line; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008067:Lipoma / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D014178:Translocation, Genetic / Q000502:physiology*
D004268:DNA-Binding Proteins; C104382:PLAG1 protein, human; D012313:RNA