Ectopic expression of truncated Sp1 transcription factor prolongs the S phase and reduces the growth rate.
The role of the transcription factor Sp1 in cell growth and survival was investigated by induced expression of its DNA-binding C-terminal fragment. Transfection of a constitutively expressed Sp1-170C plasmid construct into HeLa cells failed to produce viable clones, suggesting that this peptide interferes with cell growth. However, transfection with the muristerone A-inducible vector system produced four clones with low levels of expression of Sp1-170C. Muristerone A transiently induced higher levels of Sp1-170C, and this was accompanied by a reduced growth rate and prolongation of the S phase of the cell cycle. This is the first report that a dominant negative Sp1 can affect the cell cycle.
Chen
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F
Department of Pathology and Laboratory Medicine, UMD-New Jersey Medical School, Newark 07103, USA.
Zhang
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F
Rao
J
J
Studzinski
G P
GP
D001483:Base Sequence; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D004441:Ecdysterone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase / Q000187:drug effects; D016329:Sp1 Transcription Factor / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D011994:Recombinant Proteins; D016329:Sp1 Transcription Factor; C055348:muristerone A; D004441:Ecdysterone