Metabolism of methanol in plant cells. Carbon-13 nuclear magnetic resonance studies.
Using (13)C-NMR, we demonstrate that [(13)C]methanol readily entered sycamore (Acer pseudoplatanus L.) cells to be slowly metabolized to [3-(13)C]serine, [(13)CH(3)]methionine, and [(13)CH(3)]phosphatidylcholine. We conclude that the assimilation of [(13)C]methanol occurs through the formation of (13)CH(3)H(4)Pte-glutamate (Glu)(n) and S-adenosyl-methionine, because feeding plant cells with [3-(13)CH(3)]serine, the direct precursor of (13)CH(2)H(4)Pte-Glu(n), can perfectly mimic [(13)CH(3)]methanol for folate-mediated single-carbon metabolism. On the other hand, the metabolism of [(13)C]methanol in plant cells revealed assimilation of label into a new cellular product that was identified as [(13)CH(3)]methyl-beta-D-glucopyranoside. The de novo synthesis of methyl-beta-D-glucopyranoside induced by methanol did not require the formation of (13)CH(3)H(4)Pte-Glu(n) and was very likely catalyzed by a "transglycosylation" process.
Gout
E
E
Laboratoire de Résonance Magnétique en Biologie Métabolique, Département de Biologie Moléculaire et Structurale, CEA-38054, Grenoble cedex 9, France.
Aubert
S
S
Bligny
R
R
Rébeillé
F
F
Nonomura
A R
AR
Benson
A A
AA
Douce
R
R
D000596:Amino Acids / Q000378:metabolism; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*
D000596:Amino Acids; D002247:Carbon Isotopes; D000432:Methanol