title: Detection of Bordetella pertussis in a clinical laboratory by culture, polymerase chain reaction, and direct fluorescent antibody staining; accuracy, and cost.
abstract: Control of Bordetella pertussis in the community is hampered by slow and insensitive diagnostic tests. We therefore examined the accuracy and cost of culture, direct fluorescent antibody (DFA) staining, and PCR in a routine clinical laboratory. Six hundred thirty seven nasopharyngeal swabs and aspirates in casamino acids transport medium were cultured, stained with polyclonal (Difco), and monoclonal (BL-5 and Accu-Mab) anti-B. pertussis reagents, and amplified by an IS481-specific PCR. PCR products were detected by a hybridization-enzyme immunoassay kit (Gen-eti-k DEIA, DiaSorin), with confirmation by a second PCR in a separate laboratory. Sensitivities and specificities of culture, polyclonal DFA, monoclonal DFA, and PCR were 36 and 100%, 11.4 and 94.6%, 8.3 and 98. 4%, and 95.0 and 99.3%, respectively, with a prevalence of 15.7%. The DFA tests were the most economical, and the PCR cost was 31% higher than culture. This study suggests that with minor improvements in economy, pertussis PCR can be implemented in a clinical laboratory with marked improvement in diagnostic accuracy.
authors.affiliation: Dept of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada. tilley@duke.usak.ca
authors.forename: P A
authors.initials: PA
authors.lastname: Tilley
authors.forename: M V
authors.initials: MV
authors.lastname: Kanchana
authors.forename: I
authors.initials: I
authors.lastname: Knight
authors.forename: J
authors.initials: J
authors.lastname: Blondeau
authors.forename: N
authors.initials: N
authors.lastname: Antonishyn
authors.forename: H
authors.initials: H
authors.lastname: Deneer
mesh_terms: D001886:Bordetella pertussis / Q000235:genetics / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D003365:Costs and Cost Analysis; D019085:Fluorescent Antibody Technique, Direct / Q000191:economics; D006801:Humans; D007753:Laboratories; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000191:economics / Q000379:methods