Organophosphonate metabolism by a moderately halophilic bacterial isolate.
A Gram-negative halophile isolated from soil beneath a road gritting salt pile grew optimally at 10% (w/v) NaCl and was shown most likely to be Chromohalobacter marismortui or Pseudomonas beijerinckii on the basis of 16S rRNA analysis. The strain utilised phosphonoacetate, 2-aminoethyl-, 3-aminopropyl-, 4-aminobutyl-, methyl- and ethyl-phosphonates as phosphorus sources for growth. Differences were observed in the growth rate on different phosphonates and the range of phosphonates utilised at elevated NaCl concentrations, possibly as a result of differentially-induced transport mechanisms. An assay of cell-free extracts of 2-aminoethylphosphonate (2AEP) grown cells showed no detectable 2AEP:pyruvate aminotransferase or phosphonoacetaldehyde hydrolase activity.
Hayes
V E
VE
School of Environmental Sciences, University of Ulster, Cromore Road, Coleraine, UK.
Ternan
N G
NG
McMullan
G
G
D001673:Biodegradation, Environmental; D006090:Gram-Negative Bacteria / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D006358:Hot Temperature; D009943:Organophosphorus Compounds / Q000378:metabolism*; D009994:Osmolar Concentration; D011549:Pseudomonas / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride; D012988:Soil Microbiology
D009943:Organophosphorus Compounds; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride