Hydroxyapatite coated with heaptocyte growth factor (HGF) stimulates human osteoblasts in vitro.
We have studied in vitro the effect of a hydroxyapatite (HA) tricalcium phosphate material coated with hepatocyte growth factor (HA-HGF) on cell growth, collagen synthesis and secretion of metalloproteinases (MMPs) by human osteoblasts. Cell proliferation was stimulated when osteoblasts were incubated with untreated HA and was further increased after exposure to HA-HGF. The uptake of [3H]-proline was increased after treatment with HA. When osteoblasts were exposed to HA-HGF, collagen synthesis was increased with respect to HA. The secretion of MMPs in control cells was undetectable, but in HA and HA-HGF cells MMP 2 and MMP 9 were clearly synthesised. Our results suggest that HA can promote osteoblast activity and that HGF can further increase its bioactivity.
Zambonin
G
G
Dipartimento di Metodologia Clinica e Tecnologie Medico-Chirugiche, University of Bari, Italy.
Camerino
C
C
Greco
G
G
Patella
V
V
Moretti
B
B
Grano
M
M
D018786:Bone Substitutes; D002130:Calcium Phosphates; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D006882:Hydroxyapatites; D066298:In Vitro Techniques; D008666:Metalloendopeptidases / Q000378:metabolism; D016348:Osseointegration / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects*
D018786:Bone Substitutes; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D006882:Hydroxyapatites; C094280:hydroxyapatite-tricalciumphosphate composite; D017228:Hepatocyte Growth Factor; D003094:Collagen; D008666:Metalloendopeptidases