title: CHE-3, a cytosolic dynein heavy chain, is required for sensory cilia structure and function in Caenorhabditis elegans.
abstract: Forward genetic screens using novel assays of nematode chemotaxis to soluble compounds identified three independent transposon-insertion mutations in the gene encoding the Caenorhabditis elegans dynein heavy chain (DHC) 1b isoform. These disruptions were mapped and cloned using a newly developed PCR-based transposon display. The mutations were demonstrated to be allelic to the che-3 genetic locus. This isoform of dynein shows temporally and spatially restricted expression in ciliated sensory neurons, and mutants show progressive developmental defects of the chemosensory cilia. These results are consistent with a role for this motor protein in the process of intraflagellar transport; DHC 1b acts in concert with a number of other proteins to establish and maintain the structural integrity of the ciliated sensory endings in C. elegans.
authors.affiliation: Division of Molecular Biology, Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, Amsterdam, 1066 CX, The Netherlands.
authors.forename: S R
authors.initials: SR
authors.lastname: Wicks
authors.forename: C J
authors.initials: CJ
authors.lastname: de Vries
authors.forename: H G
authors.initials: HG
authors.lastname: van Luenen
authors.forename: R H
authors.initials: RH
authors.lastname: Plasterk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D002633:Chemotaxis / Q000235:genetics*; D002923:Cilia / Q000502:physiology*; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D012734:Disorders of Sex Development; D004398:Dyneins / Q000235:genetics / Q000502:physiology*; D005260:Female; D005407:Flagella / Q000502:physiology; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009474:Neurons / Q000502:physiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: C099884:DNAH12 protein, human; D008164:Luminescent Proteins; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D004398:Dyneins