title: Resistance to macrolides in Streptococcus pyogenes in France in pediatric patients.
abstract: A total of 1,500 recent throat isolates of Streptococcus pyogenes collected between 1996 and 1999 from children throughout France were tested for their susceptibility to erythromycin, azithromycin, josamycin, clindamycin, and streptogramin B. The erythromycin-resistant isolates were further studied for their genetic mechanism of resistance, by means of PCR. The clonality of these strains was also investigated by means of serotyping and ribotyping. In all, 6.2% of the strains were erythromycin resistant, and 3.4 and 2.8% expressed the constitutive MLS(B) and M resistance phenotypes and harbored the ermB and mefA genes, respectively; ermTR was recovered from one isolate which also harbored the ermB gene. Ten serotypes and 8 ribotypes were identified, but we identified 17 strains by combining serotyping with ribotyping. Among the eight ribotypes, the mefA gene was recovered from six clusters, one being predominant, while the ermB gene was recovered from four clusters, of which two were predominant.
authors.affiliation: Service de Microbiologie, Hôpital Robert Debré, 75019 Paris, France. edouard.bingen@rdb.ap-hop-paris.fr
authors.forename: E
authors.initials: E
authors.lastname: Bingen
authors.forename: F
authors.initials: F
authors.lastname: Fitoussi
authors.forename: C
authors.initials: C
authors.lastname: Doit
authors.forename: R
authors.initials: R
authors.lastname: Cohen
authors.forename: A
authors.initials: A
authors.lastname: Tanna
authors.forename: R
authors.initials: R
authors.lastname: George
authors.forename: C
authors.initials: C
authors.lastname: Loukil
authors.forename: N
authors.initials: N
authors.lastname: Brahimi
authors.forename: I
authors.initials: I
authors.lastname: Le Thomas
authors.forename: D
authors.initials: D
authors.lastname: Deforche
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology* / Q000627:therapeutic use; D005602:France / Q000453:epidemiology; D006801:Humans; D007223:Infant; D013290:Streptococcal Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D004917:Erythromycin