Mouse myosin X: molecular architecture and tissue expression as revealed by northern blot and in situ hybridization analyses.
The structure of the coding region of mouse myosin X cDNA was determined. The predicted protein sequence indicated an approximately 240 kDa molecular mass with 2062 amino acids. When aligned with the structure predicted for calf myosin X (GenBank Accession No. U55042), extremely highly conserved pleckstrin homology domains and a myosin tail homology 4 domain were apparent in the tail region, suggesting their importance for myosin X's function. Northern blot analysis revealed the existence of a myosin X mRNA, 8.7 kb in size, in various mouse tissues, while a similar size of human type myosin X mRNA was recognized mainly in the testis. In addition to the adult-type transcripts in mice, a smaller embryo-specific mRNA, 4.8 kb in size, was identified in early to late embryonic stages, suggesting the presence of a shorter myosin X isoform in mouse embryos. In situ hybridization experiments with mouse testis revealed that myosin X mRNA was restricted to Sertoli cells at stages VIII-X of the spermatogenesis cycle, suggesting that myosin X is implicated in the supporting cells during the spermatid morphogenesis.
Yonezawa
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S
Institute for Developmental Research, Aichi Human Service Center, Kasugai, 480-0392, Japan. yonez@inst-hsc.pref.aichi.jp
Kimura
A
A
Koshiba
S
S
Masaki
S
S
Ono
T
T
Hanai
A
A
Sonta
S
S
Kageyama
T
T
Takahashi
T
T
Moriyama
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D015152:Blotting, Northern; D002895:Chromosomes, Human, Pair 5; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009218:Myosins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D010750:Phosphoproteins / Q000737:chemistry; D017434:Protein Structure, Tertiary; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012708:Sertoli Cells / Q000378:metabolism; D015398:Signal Transduction; D016608:Talin / Q000737:chemistry; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution
D001798:Blood Proteins; C498718:MYO10 protein, human; C409064:Myo10 protein, mouse; D010750:Phosphoproteins; D016608:Talin; C039077:platelet protein P47; D009218:Myosins