Characterization of the surfaces generated by liposome binding to the modified dextran matrix of a surface plasmon resonance sensor chip.
The dextran matrix of a surface plasmon resonance (SPR) sensor chip modified with hydrophobic residues (BIAcore sensor chip L1) provides an ideal substrate for liposome adsorption. Liposomes of different lipid compositions are captured on the sensor chips by inserting these residues into the liposome membrane, thereby generating stable lipid surfaces. To gain a more detailed understanding of these surfaces, and to prove whether the liposomes stay on the matrix as single particles or form a continuous lipid layer by liposome fusion, we have investigated these materials, using atomic force microscopy (AFM) and fluorescence microscopy. Force measurements with AFM probes functionalized with bovine serum albumin (BSA) were employed to recognize liposome adsorption. Analysis of the maximal adhesive force and adhesion energy reveals a stronger interaction between BSA and the dextran matrix compared to the lipid-covered surfaces. Images generated using BSA-coated AFM tips indicated a complete and homogeneous coverage of the surface by phospholipid. Single liposomes could not be detected even at lower lipid concentrations, indicating that the liposomes fuse and form a lipid bilayer on the dextran matrix. Experiments with fluorescently labeled liposomes concurred with the AFM studies. Surfaces incubated with liposomes loaded with TRITC-labeled dextran showed no fluorescence, indicating a complete release of the encapsulated dye. In contrast, surfaces incubated with liposomes containing a fluorescently labeled lipid showed fluorescence.
Erb
E M
EM
Department of Clinical Chemistry, Lund University, University Hospital Malmö, Sweden.
Chen
X
X
Allen
S
S
Roberts
C J
CJ
Tendler
S J
SJ
Davies
M C
MC
Forsén
S
S
D000818:Animals; D002417:Cattle; D003911:Dextrans / Q000737:chemistry*; D008081:Liposomes; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties
D003911:Dextrans; D008081:Liposomes