Unpaired bases in superhelical DNA: kinetic evidence.
Kinetic analysis of the early, fast reaction of superhelical DNA with formaldehyde reveals that this region or regions is 56% "single strand like" in character. Hydrogen-tritium exchange studies coupled with other considerations show that this reaction is not due to a difference in conformational motility between form I and form II molecules, but is due to unpaired or weakly hydrogen bonded, localized region(s) of the form I allomorph of circular DNA.
Jacob
R J
RJ
Lebowitz
J
J
Printz
M P
MP
D020029:Base Pairing; D004278:DNA, Superhelical / Q000737:chemistry*; D006860:Hydrogen Bonding; D007700:Kinetics; D008956:Models, Chemical; D009690:Nucleic Acid Conformation
D004278:DNA, Superhelical