Use of 8-hydroxyquinoline-beta-D-glucuronide for presumptive identification of Shiga toxin-producing Escherichia coli O157.
8-hydroxyquinoline-beta-D-glucuronide (HQG) was used to improve the presumptive identification of Shiga toxin-producing Escherichia coli O157 (STEC O157) on sorbitol MacConkey agars (SMAC). Advantages of HQG are (i) that it is less expensive than 5-bromo-4-chloro-3-indoxyl-glucuronide; (ii) that it is visible in normal daylight and (iii) that it does not diffuse into the agar like 4-methylumbelliferryl-beta-D-glucuronide (MUG). Sixteen STEC O157 isolates, 91 bovine mastitis-associated E. coli isolates and 222 faecal E. coli isolates from apparently healthy cattle were used in this study. 4-methylumbelliferryl-beta-D-glucuronide detected beta-glucuronidase activity in more isolates than HQG (P < 0.05). On SMAC with HQG, cefixime and tellurite all STEC O157 isolates grew as cream-coloured colonies (100% sensitivity), whereas all non-STEC O157 E. coli except one grew either not at all or as purple or black colonies (99.7% specificity). No difference was found between faecal and mastitis isolates for the proportion of isolates that hydrolysed HQG or MUG or fermented sorbitol. However, significantly more mastitis isolates were able to grow in the presence of the cefixime-tellurite supplement. 8-Hydroxyquinoline-beta-D-glucuronide is a useful substrate for the identification of STEC O157 on SMAC.
Reinders
R D
RD
Center for Veterinary Public Health and Environmental Protection, Utrecht University, Utrecht, The Netherlands. r.d.reinders@vvdo.vet.uu.nl
Bijker
P G
PG
Huis In't Veld
J H
JH
Van Knapen
F
F
D000818:Animals; D002417:Cattle; D003470:Culture Media; D019453:Escherichia coli O157 / Q000254:growth & development*; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D006912:Hydroxyquinolines; D008413:Mastitis / Q000382:microbiology; D008460:Meat / Q000382:microbiology*; D013012:Sorbitol; D013691:Tellurium
D003470:Culture Media; D006912:Hydroxyquinolines; C056147:8-hydroxyquinoline glucuronide; D013012:Sorbitol; C026660:tellurous acid; D013691:Tellurium