Characterization of the relA gene of Porphyromonas gingivalis.
Based upon the nucleotide sequence of the relA gene from Escherichia coli, a gene fragment corresponding to the homologous gene from the pathogenic oral bacterium Porphyromonas gingivalis 381 was isolated by PCR and utilized to construct a relA mutant. The mutant, KS7, was defective in ribosome-mediated ppGpp formation and also in the stringent response.
Sen
K
K
Departments of Oral Biology, State University of New York, Buffalo, New York 14214, USA.
Hayashi
J
J
Kuramitsu
H K
HK
D000595:Amino Acid Sequence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006159:Guanosine Tetraphosphate / Q000096:biosynthesis; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D016966:Porphyromonas gingivalis / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D012270:Ribosomes / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D006159:Guanosine Tetraphosphate; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases