Inverse radiation dose-rate effects on somatic and germ-line mutations and DNA damage rates.
The mutagenic effect of low linear energy transfer ionizing radiation is reduced for a given dose as the dose rate (DR) is reduced to a low level, a phenomenon known as the direct DR effect. Our reanalysis of published data shows that for both somatic and germ-line mutations there is an opposite, inverse DR effect, with reduction from low to very low DR, the overall dependence of induced mutations being parabolically related to DR, with a minimum in the range of 0.1 to 1.0 cGy/min (rule 1). This general pattern can be attributed to an optimal induction of error-free DNA repair in a DR region of minimal mutability (MMDR region). The diminished activation of repair at very low DRs may reflect a low ratio of induced ("signal") to spontaneous background DNA damage ("noise"). Because two common DNA lesions, 8-oxoguanine and thymine glycol, were already known to activate repair in irradiated mammalian cells, we estimated how their rates of production are altered upon radiation exposure in the MMDR region. For these and other abundant lesions (abasic sites and single-strand breaks), the DNA damage rate increment in the MMDR region is in the range of 10% to 100% (rule 2). These estimates suggest a genetically programmed optimatization of response to radiation in the MMDR region.
Vilenchik
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The Sally Balin Medical Center, Media, PA 19063, USA.
Knudson
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D000818:Animals; D004247:DNA / Q000737:chemistry / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D018095:Germ-Line Mutation / Q000528:radiation effects*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007940:Leukemia L5178 / Q000201:enzymology; D018499:Linear Energy Transfer; D008297:Male; D008322:Mammals; D051379:Mice; D010084:Oxidation-Reduction; D011839:Radiation, Ionizing; D013093:Spermatogonia / Q000528:radiation effects*; D013234:Stem Cells / Q000528:radiation effects; D013269:Stochastic Processes; D013816:Thermodynamics; D014407:Tumor Cells, Cultured
D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase