Transient over-expression of NGFI-A gene suppresses NGF-induced neurite outgrowth in PCI2 cells.
Using an inducible gene expression system (Tet-ON system), the role of NGFI-A gene during the neuronal differentiation of PCI2 cells was examined. When NGFI-A was transiently over-expressed, no obvious effects on cell proliferation or neurite outgrowth were observed. Interestingly, however, NGFI-A over-expression resulted in significant retardation in NGF-induced neurite outgrowth. Similar suppressive effects were observed also on the v-K-ras-induced neurite outgrowth. These results raise the possibility that NGFI-A protein may play some negative role in NGF signaling.
Matsumoto
T
T
Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Japan.
Akiyama
N
N
Kizaka-Kondoh
S
S
Noda
M
M
D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D051766:Early Growth Response Protein 1; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005822:Genetic Vectors; D017874:Immediate-Early Proteins; D020932:Nerve Growth Factor / Q000378:metabolism* / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D016716:PC12 Cells; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D014157:Transcription Factors; D020932:Nerve Growth Factor