title: Occurrence of aflatoxin M1 in Korean dairy products determined by ELISA and HPLC.
abstract: The occurrence of aflatoxin M1 (AFM1) in pasteurized milk and dairy products was investigated by using direct competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). The recoveries of AFM1 from the samples spiked at levels between 5 and 500 pg/ml were 88.0-106.5% for pasteurized milk and 84.0-94.0% for yoghurt by ELISA. By HPLC, the recoveries were 103-120% for pasteurized milk and 87.0-93.0% for yoghurt. The limits of detection were found to be 2 pg/ml by ELISA and 10 pg/ml by HPLC. Among a total of 180 samples collected in Seoul, Korea, the incidence of AFM1 in pasteurized milk, infant formula, powdered milk and yoghurt was 76, 85, 75, and 83%, respectively, with a mean concentration of 18, 46, 200, and 29 pg/g, respectively, when determined by ELISA. These results obtained by ELISA were closely related to those by HPLC for AFM1 (r2 = 0.9783).
authors.affiliation: Graduate School of Biotechnology, Korea University, Seoul, Korea.
authors.forename: E K
authors.initials: EK
authors.lastname: Kim
authors.forename: D H
authors.initials: DH
authors.lastname: Shon
authors.forename: D
authors.initials: D
authors.lastname: Ryu
authors.forename: J W
authors.initials: JW
authors.lastname: Park
authors.forename: H J
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Y B
authors.initials: YB
authors.lastname: Kim
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination; D007225:Infant Food / Q000032:analysis; D007723:Korea; D008892:Milk / Q000737:chemistry; D015014:Yogurt / Q000032:analysis
chemical_list: D016607:Aflatoxin M1