The inhibitory effect of the 5' untranslated region of muscle acylphosphatase mRNA on protein expression is relieved during cell differentiation.
Previous experiments suggested that the upstream AUG triplet present in the 5' untranslated region (UTR) of muscle acylphosphatase mRNA is involved in the regulation of protein expression. In this paper, we study the involvement of the 5'UTR secondary structure and upstream peptide on mRNA stability and protein translation. Our data, obtained using deletion and frame-shift mutants, demonstrate that the 5'UTR controls protein expression regulating translation together with mRNA stability. Furthermore, we demonstrate that the inhibitory effect of the 5'UTR of muscle acylphosphatase is relieved during the differentiation process in agreement with previous data reporting an increase of acylphosphatase content during cell differentiation. Finally, UV cross-linking experiments show that specific mRNA-binding proteins are associated with the 5'UTR of the muscle acylphosphatase mRNA.
Fiaschi
T
T
Dipartimento di Scienze Biochimiche, Università di Firenze, viale Morgagni 50, 50134, Florence, Italy.
Chiarugi
P
P
Veggi
D
D
Raugei
G
G
Ramponi
G
G
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017766:Acid Anhydride Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016590:Aphidicolin / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D018387:Codon, Initiator / Q000235:genetics; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D016368:Frameshift Mutation / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D020014:K562 Cells; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008970:Molecular Weight; D009132:Muscles / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics*; D020871:RNA Stability / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D000097024:Acylphosphatase
D020121:5' Untranslated Regions; D017766:Acid Anhydride Hydrolases; D016590:Aphidicolin; D018387:Codon, Initiator; D016601:RNA-Binding Proteins; D013755:Tetradecanoylphorbol Acetate; D000097024:Acylphosphatase