title: Protein oxidation in response to increased transcriptional or translational errors.
abstract: In this study, we show a correlation between synthesis of aberrant proteins and their oxidative modification. The level of aberrant proteins was elevated in Escherichia coli cultures by decreasing transcriptional or translational fidelity using specific mutations or drugs. Protein carbonylation, an oxidative modification, increased in parallel to the induction of the heat shock chaperone GroEL. As the protein turnover rates and level of intracellular oxidative stress remained unchanged, it appears that carbonylation results from the increased susceptibility of the misfolded proteins. These studies show that the cellular protein oxidation is not limited only by available reactive oxygen species, but by the levels of aberrant proteins. Thus, protein oxidation seen in aging cells may be the consequence also of reduced transcriptional/translational fidelity, and protein structures appear to have evolved to minimize oxidative damage. In addition, we discuss the possibility that carbonylation, being an unrepairable protein modification, may serve as a tagging system to shunt misfolded proteins between pathways of refolding by chaperones or the proteolytic apparatus.
authors.affiliation: Department of Cell and Molecular Biology-Microbiology, Göteborg University, Box 462, 405 30 Göteborg, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Dukan
authors.forename: A
authors.initials: A
authors.lastname: Farewell
authors.forename: M
authors.initials: M
authors.lastname: Ballesteros
authors.forename: F
authors.initials: F
authors.lastname: Taddei
authors.forename: M
authors.initials: M
authors.lastname: Radman
authors.forename: T
authors.initials: T
authors.lastname: Nyström
mesh_terms: D000215:Acylation; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis; D017510:Protein Folding; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D013481:Superoxides