Preliminary results of myoblast injection into the urethra and bladder wall: a possible method for the treatment of stress urinary incontinence and impaired detrusor contractility.
The purpose of this study is to explore the feasibility of myoblasts, the precursors of muscle fibers, injected periurethrally as a potential treatment of stress urinary incontinence. We also studied myoblast injection into the bladder wall to potentially improve detrusor contractility. A myoblast cell line was transduced with adenovirus carrying the expression of the beta-galactosidase reporter gene while in culture. The cells were incubated with fluorescent latex microspheres (FLMs) to follow the outcome of the injected cells. The tissue was harvested 3-4 days after injection; sectioned, fixed, assayed for beta-galactosidase expression, and counterstained with H+E. Photographs of the slides were taken under light and fluorescence microscopy. We have noted a large number of cells expressing beta-galactosidase and containing FLMs in the urethral and bladder walls under fluorescent microscopy (8 animals). Many regenerative myofibers expressing beta-galactosidase were also seen in the urethral and bladder walls. The fusion of injected myoblasts to form myotubes was seen in both the urethral and bladder walls. The introduction of myoblasts into the urethral and bladder wall is feasible and results in formation of myotubes and myofibers in the smooth muscle layers of the lower urinary tract. We hypothesize that myoblast injections can be used as a non-allergenic agent to enhance urethral closure and bladder function.
Chancellor
M B
MB
Division of Urologic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.
Yokoyama
T
T
Tirney
S
S
Mattes
C E
CE
Ozawa
H
H
Yoshimura
N
N
de Groat
W C
WC
Huard
J
J
D000818:Animals; D002478:Cells, Cultured; D005240:Feasibility Studies; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D007267:Injections; D051379:Mice; D018101:Mice, Inbred mdx; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000503:physiopathology; D009132:Muscles / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014521:Urethra; D001743:Urinary Bladder; D014550:Urinary Incontinence, Stress / Q000628:therapy*