title: Electrochemical investigation of cellobiose oxidation by cellobiose dehydrogenase in the presence of cytochrome c as mediator.
abstract: An important aspect of the cytochrome c electrochemistry is the possibility of coupling the 'heterogeneous reactions' with other redox enzymes. Cellobiose dehydrogenase, a 89170 Da glycoprotein that contains both FAD and a b-type haem as prosthetic groups, donates electrons to a number of acceptors, including cytochrome c. While haem b is surrounded mainly by acidic amino acids, cytochrome c displays positive charged lysine groups around the haem site. Thus a fast reaction between both proteins is explicable. In the presence of cellobiose, a catalytic current was observed, owing to the interaction of cellobiose dehydrogenase with electrostatically adsorbed cytochrome c. Adsorption of cytochrome c provides a technological model surface for vectorial electron transfer.
authors.affiliation: University of Potsdam, Institute of Biochemistry and Molecular Physiology, Luckenwalde, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Fridman
authors.forename: U
authors.initials: U
authors.lastname: Wollenberger
authors.forename: V
authors.initials: V
authors.lastname: Bogdanovskaya
authors.forename: F
authors.initials: F
authors.lastname: Lisdat
authors.forename: T
authors.initials: T
authors.lastname: Ruzgas
authors.forename: A
authors.initials: A
authors.lastname: Lindgren
authors.forename: L
authors.initials: L
authors.lastname: Gorton
authors.forename: F W
authors.initials: FW
authors.lastname: Scheller
mesh_terms: D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism* / Q000502:physiology*; D004563:Electrochemistry; D004583:Electrons; D007700:Kinetics; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D020075:Phanerochaete / Q000201:enzymology; D011485:Protein Binding; D013053:Spectrophotometry
chemical_list: D003574:Cytochrome c Group; D002475:Cellobiose; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D010100:Oxygen