HZwint-1, a novel human kinetochore component that interacts with HZW10.
HZwint-1 (Human ZW10 interacting protein-1) was identified in a yeast two hybrid screen for proteins that interact with HZW10. HZwint-1 cDNA encodes a 43 kDa protein predicted to contain an extended coiled-coil domain. Immunofluorescence studies with sera raised against HZwint-1 protein revealed strong kinetochore staining in nocodazole-arrested chromosome spreads. This signal co-localizes at the kinetochore with HZW10, at a position slightly outside of the central part of the centromere as revealed by staining with a CREST serum. The kinetochore localization of HZwint-1 has been confirmed by following GFP fluorescence in HeLa cells transiently transfected with a plasmid encoding a GFP/HZwint-1 fusion protein. In cycling HeLa cells, HZwint-1 localizes to the kinetochore of prophase HeLa cells prior to HZW10 localization, and remains at the kinetochore until late in anaphase. This localization pattern, combined with the two-hybrid results, suggests that HZwint-1 may play a role in targeting HZW10 to the kinetochore at prometaphase. HZwint-1 was also found to localize to neocentromeres and to the active centromere of dicentric chromosomes. HZwint-1 thus appears to associate with all active centromeres, implying that it plays an important role in correct centromere function.
Starr
D A
DA
Section of Genetics and Development, Cornell University, Ithaca, NY 14853, USA.
Saffery
R
R
Li
Z
Z
Simpson
A E
AE
Choo
K H
KH
Yen
T J
TJ
Goldberg
M L
ML
D000595:Amino Acid Sequence; D001483:Base Sequence; D015415:Biomarkers; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins; D005820:Genetic Testing; D006367:HeLa Cells; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D018386:Kinetochores / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D020798:Two-Hybrid System Techniques
D015415:Biomarkers; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D019476:Insect Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C413695:ZWINT protein, human; C076055:Zw10 protein, Drosophila