Protection of myocardial mitochondria against oxidative damage by selenium-containing abzyme m4G3.
Selenium-containing abzyme (m4G3) was prepared and its protection of myocardial mitochondria against oxidative damage was studied using the swelling of mitochondria, quantity of lipid peroxidation products, and change in cytochrome-c oxidase activity as a measure of mitochondrial damage. The results showed that m4G3 could inhibit mitochondrial damage caused by the hypoxanthine-xanthine oxidase system in vitro. Electronic spin resonance (ESR) studies demonstrated that m4G3 could decrease the amount of free radicals generated in the damage system.
Qi
D H
DH
National Laboratory of Enzyme Engineering, Jilin University, Changchun, China.
Luo
G M
GM
Zhou
L
L
Yang
T S
TS
D000818:Animals; D017151:Antibodies, Catalytic / Q000494:pharmacology*; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D003576:Electron Transport Complex IV / Q000378:metabolism; D005609:Free Radicals; D005979:Glutathione Peroxidase / Q000494:pharmacology*; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D012643:Selenium / Q000494:pharmacology*; D013997:Time Factors; D014969:Xanthine Oxidase / Q000378:metabolism
D017151:Antibodies, Catalytic; D005609:Free Radicals; C409405:m4G3 abzyme; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D014969:Xanthine Oxidase; D003576:Electron Transport Complex IV; D012643:Selenium