Plasma concentration of urea, ammonia, glutamine around calving, and the relation of hepatic triglyceride, to plasma ammonia removal and blood acid-base balance.
Two experiments were conducted to test the following two hypotheses: 1) fatty liver could hamper hepatic conversion of ammonia to urea and increase circulating ammonia or Gln% [Gln% = Gln x 100/(Gln + Glu)] in cows around parturition; 2) decreased ureagenesis might cause alkalosis and in turn reduce blood Ca. In the first experiment, 14 Holstein cows were monitored from 27 d prepartum to 35 d postpartum. There was a rise in circulating ammonia and Gln% at calving, suggesting an increase in ammonia passing to and through the liver. Stepwise regression analysis revealed the following relationship for plasma samples at 22 h and liver triglyceride at 2 d postpartum: ammonia (microM) = 32.1+/-0.89 triglyceride (% DM), Gln% = 71.2 + 0.23 triglyceride (% DM) + 1.31 urea (mM). The positive correlation between liver triglyceride and plasma ammonia and Gln% suggests that hepatic triglyceride accumulation might inhibit ureagenesis, thereby increasing ammonia concentration at the perivenous hepatocytes where Gln synthesis occurs and increasing ammonia concentration in blood leaving the liver. In the second experiment, 28 rats were used to determine whether hepatic triglyceride accumulation, induced by choline deficiency, affects urinary ammonia N and blood pH homeostasis. There was a trend for a positive correlation between urinary ammonia N and liver triglyceride. No correlation between liver triglyceride and blood pH, bicarbonate, pCO2 or plasma Ca was found. In conclusion, hepatic triglyceride accumulation may inhibit ureagenesis and result in increased circulating ammonia, Gln% and urinary ammonia N in vivo. Hepatic triglyceride accumulation did not affect blood pH homeostasis.
Zhu
L H
LH
Department of Dairy Science, University of Wisconsin, Madison 53706, USA.
Armentano
L E
LE
Bremmer
D R
DR
Grummer
R R
RR
Bertics
S J
SJ
D000136:Acid-Base Equilibrium; D000641:Ammonia / Q000097:blood*; D000818:Animals; D002118:Calcium / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism*; D002796:Choline Deficiency; D004435:Eating; D005234:Fatty Liver / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D005260:Female; D005973:Glutamine / Q000097:blood*; D007743:Labor, Obstetric; D007774:Lactation; D008099:Liver / Q000378:metabolism; D011247:Pregnancy; D012044:Regression Analysis; D014280:Triglycerides / Q000378:metabolism; D014508:Urea / Q000097:blood*; D015430:Weight Gain
D014280:Triglycerides; D005973:Glutamine; D000641:Ammonia; D014508:Urea; D002118:Calcium