Immunolocalisation of myosin-V in the enteric nervous system of the rat.
We show here the localisation of myosin-V in whole mount preparations of the mucous-submucous and the muscular layers of rat small intestine by using an affinity purified antibody specific to the tail domain of myosin-V. Myosin-V immunostaining was intense in the submucous and myenteric nervous plexuses, allowing the visualisation of neuronal cell bodies and fibres. Western blots of total muscle layers homogenates detected with the same antibody revealed a single band of the expected size for myosin-V. Understanding the cellular localisation and function of this class of myosin is an important challenge and the accessibility and simplicity of the enteric nervous system as compared to the central nervous system, makes the digestive tract an attractive model for studying possible functional roles of myosin-V in neurotransmission and neuroplasticity.
Drengk
A C
AC
Department of Morphology of the Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Brazil.
Kajiwara
J K
JK
Garcia
S B
SB
Carmo
V S
VS
Larson
R E
RE
Zucoloto
S
S
Espreafico
E M
EM
D000818:Animals; D015153:Blotting, Western; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D017615:Enteric Nervous System / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism; D024701:Myosin Type V; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D013368:Submucous Plexus / Q000166:cytology / Q000378:metabolism
D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; C078829:chicken brain myosin-V p190; D024701:Myosin Type V