RAPD-fingerprinting of Serratia marcescens after formaldehyde inactivation of DNase activity.
Serratia marcescens strains commonly cause hospital outbreaks. A random amplified polymorphic DNA (RAPD) assay was applied to the epidemiology of Ser. marcescens using formaldehyde fixation and boiling of bacterial cells for DNA extraction. The method preserved bacterial DNA and gave optimal results of RAPD-fingerprinting, facilitating the investigation of hospital infections caused by Ser. marcescens.
Polyzou
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A
Department of Microbiology, Hippokration General Hospital, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Sofianou
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D
Pournaras
S
S
Tsakris
A
A
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D005557:Formaldehyde / Q000494:pharmacology*; D006801:Humans; D012706:Serratia marcescens / Q000187:drug effects / Q000235:genetics*
D004269:DNA, Bacterial; D005557:Formaldehyde