Multiplex PCR detection of Campylobacter jejuni and Arcobacter butzleri in food products.
Arcobacter is a recently described species, previously considered part of the Campylobacter family. A sensitive assay such as that provided by PCR could help to distinguish the closely related Arcobacter from Campylobacter. A PCR method to specifically detect both Campylobacter jejuni and Arcobacter butzleri in the same reaction tube has been developed. C. jejuni and A. butzleri were inoculated into a range of dairy products, raw and ready-to-eat foods. The presence of these two organisms was detected in these test foods by the multiplex PCR assay. A product of 159 bp was apparent when C. jejuni was present, while a 1223 bp product was seen when A. butzleri was present. When both organisms were present, both bands could be detected on the agarose gel. All organisms were confirmed by standard microbiological methods. There was complete agreement between the PCR and standard methods. This PCR assay will allow detection of both organisms within the same PCR tube and can be performed within an 8 h day. The presence of these two human pathogens, which are difficult to distinguish by standard biochemical methods, can now be identified using this PCR assay.
Winters
D K
DK
Department of Poultry Science, University of Arkansas, Fayetteville 72701, USA. dwinters@comp.uark.edu
Slavik
M F
MF
D000818:Animals; D020640:Arcobacter / Q000235:genetics / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D003611:Dairy Products / Q000382:microbiology; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology; D008461:Meat Products / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D014675:Vegetables / Q000382:microbiology
D017931:DNA Primers