Enterocyte expression of interleukin 7 induces development of gammadelta T cells and Peyer's patches.
The intestinal mucosa is suggested to support extrathymic T cell development, particularly for T cell receptor (TCR)-gammadelta intraepithelial lymphocytes (IELs). TCR-gammadelta cell development requires interleukin (IL)-7; IL-7(-/)- or IL-7 receptor(-/)- mice lack TCR-gammadelta cells. Using the intestinal fatty acid binding protein (iFABP) promoter, we reinstated expression of IL-7 to mature enterocytes of IL-7(-/)- mice (iFABP-IL7). In iFABP-IL7 mice, TCR-gammadelta IELs were restored, as were cryptopatches and Peyer's patches. TCR-gammadelta cells remained absent from all other tissues. Likewise, T cell development in thymus and B cell maturation in the bone marrow and spleen retained the IL-7(-/)- phenotype. Thus, IL-7 expression by enterocytes was sufficient for extrathymic development of TCR-gammadelta cells in situ within the intestinal epithelium and was crucial for organization of mucosal lymphoid tissue.
Laky
K
K
Department of Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030-1310, USA.
Lefrançois
L
L
Lingenheld
E G
EG
Ishikawa
H
H
Lewis
J M
JM
Olson
S
S
Suzuki
K
K
Tigelaar
R E
RE
Puddington
L
L
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology; D020895:Enterocytes / Q000276:immunology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018858:Germinal Center / Q000166:cytology; D015851:Interleukin-7 / Q000235:genetics / Q000276:immunology*; D007421:Intestine, Small / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D018996:Myelin P2 Protein / Q000235:genetics; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014018:Tissue Distribution; D019076:Transgenes
D002352:Carrier Proteins; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D015851:Interleukin-7; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011993:Recombinant Fusion Proteins