Inhibitory effect of stromal cell derived factor-1 on the replication of divergent strains of feline immunodeficiency virus in a feline T-lymphoid cell line.
The effect of a CXC-chemokine, stromal cell derived factor-1 (SDF-1), on the replication of divergent strains of feline immunodeficiency virus (FIV) was examined in order to identify the mechanism of cell entry of FIV. A chemotaxis assay, using a modified Boyden chamber method, confirmed the biological activity of recombinant human (rh) SDF-1 for a feline T-lymphoid cell line (Kumi-1). The viral replication of FIV, as measured by the reverse transcriptase (RT) activity in the culture supernatant, was significantly suppressed by addition of rhSDF-1 in a dose-dependent manner in Kumi-1 cells. Furthermore, PCR analysis of the FIV proviral genome indicated that the inhibitory effect of rhSDF-1 on the replication of FIV in Kumi-1 cells was due to the inhibitory effect in the early event of replication. The inhibitory effect on viral replication by exogenous rhSDF-1 was shown for four divergent FIV isolates of subtypes A, B, and D in Kumi-1 cells.
Endo
Y
Y
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.
Goto
Y
Y
Nishimura
Y
Y
Mizuno
T
T
Watari
T
T
Hasegawa
A
A
Hohdatsu
T
T
Koyama
H
H
Tsujimoto
H
H
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002415:Cats; D002460:Cell Line; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D016207:Cytokines / Q000502:physiology; D014644:Genetic Variation; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000254:growth & development*; D007166:Immunosuppressive Agents / Q000494:pharmacology; D019718:Receptors, CXCR4 / Q000096:biosynthesis; D017154:Stromal Cells / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000821:virology*; D014779:Virus Replication / Q000235:genetics / Q000276:immunology*
D000998:Antiviral Agents; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D016207:Cytokines; D007166:Immunosuppressive Agents; D019718:Receptors, CXCR4