Increased cytotoxicity of cadmium in fibroblasts lacking c-fos.
Cadmium has been known to induce the expression of the c-fos gene in various cell types including fibroblasts. To clarify the biological significance of c-fos induction by cadmium, mouse 3T3-like fibroblasts lacking c-fos were exposed to cadmium, and the resultant cellular damage was assayed by WST-8 (4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio] -1, 3-benzene disulfonate sodium salt) conversion, trypan blue exclusion, or lactate dehydrogenase leakage. The c-fos-deficient cells (f1 and f10) were affected more severely than the wild-type cells (NIH 3T3 and f20) with respect to both cell growth and cellular damage following exposure to 10 or 20 microM cadmium chloride. These results suggest that c-fos may play a protective role against the cytotoxic effects of cadmium at least in these 3T3-like fibroblasts.
Matsuoka
M
M
Department of Environmental Toxicology, University of Occupational and Environmental Health, Kitakyushu, Japan. masatomm@med.uoeh-u.ac.jp
Wispriyono
B
B
Igisu
H
H
D016475:3T3 Cells; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D016760:Proto-Oncogene Proteins c-fos / Q000172:deficiency / Q000378:metabolism*; D014343:Trypan Blue / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; D002104:Cadmium; D014343:Trypan Blue