[A comparison of the yield of Vi and O antigens during the adaptation of Salmonella typhi strains to cultivation under starvation conditions].
S. typhi strains Ty(2)4446 and Vi-1S underwent multiple passages in f synthetic liquid starvation culture medium consisting of water with salts and glucose added. In the process of the adaptation of the cultures to these stress conditions (starvation stress) the increasing yield of biomass from passage to passage was observed. Differences in the accumulation of Vi- and O-antigens were noted in two strains under study. In the cultures of strain Ty(2)4446 an insignificant increase in the antigen content from passage to passage was observed, while in the cultures of strain Vi-1S an increase in the content of Vi- and O-antigens was 4- to 5-fold. With the adaptation of the culture the Vi-antigen to O-antigen ratio changed from 1:57 to 1:20 for strain Ty(2)4446 and from 1:2.7 tp 1:2.2 for strain Vi-1S. Strain Ty(2)4446 had an advantage over strain Vi-1S with respect to the synthesis of Vi-antigen. These data are indicative of the expediency of using not only strain Ty(2)4446, but also strain Vi-1S for the preparation of typhoid vaccine, especially the one based on Vi-antigen.
Basnak'ian
I A
IA
Mechnikov Research Institute for Vaccines and Sera, Central Research Institute of Transfusion Medicine and Medical Engineering, Moscow, Russia.
Aleksakhina
N N
NN
L'vov
V L
VL
Sukhanov
Iu S
IuS
Alekseev
V A
VA
D000222:Adaptation, Physiological / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D016667:Bacterial Capsules / Q000276:immunology*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel / Q000706:statistics & numerical data; D006385:Hemagglutination Inhibition Tests / Q000706:statistics & numerical data; D006863:Hydrogen-Ion Concentration; D019081:O Antigens / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012485:Salmonella typhi / Q000254:growth & development / Q000276:immunology*; D012692:Serial Passage; D013997:Time Factors
D000942:Antigens, Bacterial; D003470:Culture Media; D019081:O Antigens; D011135:Polysaccharides, Bacterial