The CUP1 promoter of Saccharomyces cerevisiae is inducible by copper in Pichia pastoris.
We report the construction of a Pichia pastoris integrating vector which contains the inducible CUP1 promoter from Saccharomyces cerevisiae. We show that the promoter is indeed inducible by copper when used in P. pastoris and that the level of induction is dependent on the amount of copper in the medium.
Koller
A
A
Department of Biology, University of California San Diego, La Jolla, CA 92093-0322, USA.
Valesco
J
J
Subramani
S
S
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D003470:Culture Media; D005656:Fungal Proteins; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D000074401:Peroxins; D010843:Pichia / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases; D001616:beta-Galactosidase / Q000378:metabolism
C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; D003470:Culture Media; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C492057:PEX10 protein, S cerevisiae; D000074401:Peroxins; C120446:Pex22 protein, Pichia pastoris; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; C007121:copper thionein; D003300:Copper; D008668:Metallothionein; D044767:Ubiquitin-Protein Ligases; D001616:beta-Galactosidase