Molecular genetic study of the gutter shaped root (GSR) on mouse chromosome 17.
In this study, a genetic factor affecting the appearance of the gutter-shaped root (GSR) in mice was examined using two inbred strains of mice, C57L/J and C57BL/6J, and the genetic crosses of these strains. 120 Mit markers, which exist on mouse chromosome 17, have been investigated to select polymorphic markers between the two strains. Nine Mit markers with polymorphism between the two strains were obtained using polymerase chain reaction (PCR). To detect the individual genotype of 66 backcross mice, simple sequence length A polymorphism (SSLP) analysis was performed. From the results of individual genotyping using 9 informative markers, the highest linkage was determined at D17Mit260. Based on these findings, it was suggested that the gene causing the mouse GSR might be located on mouse chromosome 17 and nearby D17Mit260.
Matsune
K
K
Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
D000818:Animals; D002874:Chromosome Mapping; D008040:Genetic Linkage; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014071:Tooth Abnormalities / Q000235:genetics*; D014092:Tooth Root / Q000002:abnormalities*