title: Glutathione, but not transcription factor Yap1, is required for carbon source-dependent resistance to oxidative stress in Saccharomyces cerevisiae.
abstract: Resistance of haploid yeast to hydrogen peroxide and to tert-butylhydroperoxide strongly increases when 4% glucose is replaced by glycerol or ethanol as the carbon source of the complex medium. Using a GSH1-promoter-lacZ-fusion reporter construct we could demonstrate that GSH1 is one of the genes that are up-regulated during the shift from fermentative to oxidative metabolism. A gsh1 mutant did not exhibit respiratory growth resistance to H2O2, whereas it was only slightly impaired in acquiring resistance against t-BOOH in the same experimental conditions. An isogenic deltayap1 mutant, although more sensitive to oxidative stress than the wild-type (WT), could increase resistance to both peroxides by a similar factor as observed for the WT when shifted from 4% glucose to a non-fermentable carbon source. This indicates that in this case induction of resistance to oxidative stress is independent from Yap1 and from the Yap1-mediated stress response via the STRE motif.
authors.affiliation: Departamento de Biofísica, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: Maris
authors.forename: A L
authors.initials: AL
authors.lastname: Kern
authors.forename: J N
authors.initials: JN
authors.lastname: Picada
authors.forename: F
authors.initials: F
authors.lastname: Boccardi
authors.forename: M
authors.initials: M
authors.lastname: Brendel
authors.forename: J A
authors.initials: JA
authors.lastname: Henriques
mesh_terms: D000222:Adaptation, Physiological; D000438:Alcohols / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D000431:Ethanol / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D005721:Glutamate-Cysteine Ligase / Q000235:genetics; D005978:Glutathione / Q000502:physiology*; D005990:Glycerol / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007763:Lac Operon / Q000235:genetics; D009154:Mutation; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D000438:Alcohols; D003470:Culture Media; D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D000431:Ethanol; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione; D005947:Glucose; D005990:Glycerol