[The changes and significance of the intensity of transcription of endothelial nitric oxide synthase-mRNA in placental tissue from cases of pregnancy induced hypertension].
OBJECTIVE
To determine the cause of decreasd expression of endothelial nitric oxide synthase (eNOS) in placental tissue from cases of pregnancy induced hypertension (PIH).


METHODS
9 placentae of normal pregnant women and 12 placentae of patients with PIH were studied. eNOS-mRNA was measured in placental tissue by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
(1) There was eNOS-mRNA in placental tissue of normal pregnancy as well as PIH cases. (2). The intensity of transcription of eNOS-mRNA in placental tissue of PIH cases decreased significantly when compared with that of normal pregnancy (P < 0.05).


CONCLUSIONS
The decrease of the intensity of transcription of eNOS-mRNA in placental tissue of PIH patients may result in reduction of expression of eNOS in placental tissue of PIH.
Zhou
R
R
Second Affiliated Hospital, West China University of Medical Sciences, Chengdu.
Li
W
W
Cao
Z
Z
D005260:Female; D006801:Humans; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052250:Nitric Oxide Synthase Type III; D010920:Placenta / Q000201:enzymology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011225:Pre-Eclampsia / Q000201:enzymology* / Q000209:etiology; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
D012333:RNA, Messenger; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III