Quantitative cytology in ovarian carcinoma ascitic fluids.
OBJECTIVE
To assess the value of quantitative methods in the differential diagnosis between ovarian carcinoma cells and mesothelial cells in ascitic fluids.


METHODS
Ninety ascitic fluid samples, previously reported as positive for ovarian carcinoma (30 cases), suspicious for malignancy (30) and negative for malignancy, containing only reactive mesothelial cells (30), were retrieved from the files. In each of these specimens the nuclear area, perimeter, roundness and shape coefficient of 100 cells were determined at 630 x magnification. Statistical analysis was performed using analysis of variance and, for multiple comparisons, the Student-Newman-Keuls technique.


RESULTS
Mean values for nuclear area and perimeter were higher in malignant cells as compared to reactive mesothelial cells, whereas those for roundness and shape coefficients were lower. All differences were statistically significant, the former two at a .05 level and the latter at the .001 level.


CONCLUSIONS
Quantitative methods can reliably support the differential diagnosis between ovarian carcinoma cells and mesothelial cells in ascitic fluid specimens.
Kalogeraki
A
A
Department of Pathology, University of Crete Medical School, Herakleion, Greece.
Panayiotides
J
J
Tzardi
M
M
Tamiolakis
D
D
Datseris
G
G
Vlachonikolis
J
J
Delides
G S
GS
D000368:Aged; D000704:Analysis of Variance; D001202:Ascitic Fluid / Q000166:cytology / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology; D048429:Cell Size; D003937:Diagnosis, Differential; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D019044:Image Cytometry / Q000379:methods*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D011237:Predictive Value of Tests