Secretory leukocyte protease inhibitor inhibits infection of monocytes and lymphocytes with human immunodeficiency virus type 1 but does not interfere with transcytosis of cell-associated virus across tight epithelial barriers.
In the present study, we demonstrate that recombinant human secretory leukocyte protease inhibitor (rhSLPI) inhibits infection of lymphocyte- and monocyte-derived tumor cell lines and peripheral blood lymphocytes with laboratory-adapted isolates and with the primary isolate, NDK, of free human immunodeficiency virus type 1 (HIV-1). In contrast, rhSLPI did not exhibit inhibitory activity toward transcytosis of cell-associated HIV-1 through a tight monolayer of endometrial epithelial cells. These observations indicate that the inhibitory effect of SLPI is restricted to free HIV-1 in corporal fluids.
Hocini
H
H
Unité INSERM U430, Paris, France.
Becquart
P
P
Bouhlal
H
H
Adle-Biassette
H
H
Kazatchkine
M D
MD
Bélec
L
L
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control / Q000635:transmission*; D002584:Cervix Uteri / Q000166:cytology / Q000821:virology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D005260:Female; D015870:Gene Expression / Q000276:immunology; D018023:HIV Seronegativity; D015497:HIV-1; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000821:virology*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D053481:Secretory Leukocyte Peptidase Inhibitor; D015842:Serine Proteinase Inhibitors / Q000276:immunology / Q000494:pharmacology*; D019108:Tight Junctions / Q000378:metabolism / Q000821:virology
D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor; D015842:Serine Proteinase Inhibitors