Efficient induction of peptide-specific cytotoxic T lymphocytes by LPS-activated spleen cells.
Lipopolysaccharides of gram-negative bacteria are potent activators of B cells, dendritic cells and monocytes/macrophages. We have investigated the use of LPS-activated spleen cells as antigen-presenting cells to induce CD8+ cytotoxic T lymphocytes in vivo that are reactive to MHC class I binding peptides. Compared with resting spleen cells, CTL induction was more efficient and less variable for different peptides with LPS-activated spleen cells. Cytotoxic responses were specific for the immunized peptides and contained high affinity CD8+ T cells. The removal of dendritic cells and monocytes/macrophages by Sephadex G10 column did not show profound effects on CTL induction, indicating that B-cell blasts were largely responsible. This easily accessible method should facilitate the screening of MHC class I binding peptides to determine whether or not the host's T-cell repertoire contains reactive T cells.
Kakugawa
K
K
Department of Biophysics, Kyoto University, Japan.
Udaka
K
K
Nakashima
K
K
Inaba
K
K
Oka
Y
Y
Sugiyama
H
H
Tamamura
H
H
Yamagishi
H
H
D000818:Animals; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015395:Histocompatibility Antigens Class I; D008070:Lipopolysaccharides; D010455:Peptides