title: Plasma hyaluronan-binding protein is a serine protease.
abstract: CTCF is an essential factor for optimal transcription from the amyloid beta-protein precursor promoter. A proteolytic activity detected in bovine, rabbit, horse, and human serum cleaves CTCF at three major sites, resulting in a modified mobility shift pattern of the fragments that retain DNA binding ability. The protease was purified to electrophoretic homogeneity, partially sequenced, and identified as the plasma hyaluronan-binding protein. The proteolytic activity was selectively abolished by various serine protease inhibitors, including the Kunitz-type protease inhibitor domain of amyloid beta-protein precursor. Reduction with beta-mercaptoethanol showed that the 70-kDa protein consists of two polypeptides with apparent molecular masses of 44 and 30 kDa. The serine protease domain was localized to the 30-kDa polypeptide as determined by [(3)H]diisopropylfluorophosphate binding.
authors.affiliation: Department of Psychiatry and Behavioral Science, State University of New York at Stony Brook, Stony Brook, New York 11794-8101, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Vostrov
authors.forename: W W
authors.initials: WW
authors.lastname: Quitschke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000076246:CCCTC-Binding Factor; D002417:Cattle; D002850:Chromatography, Gel; D004268:DNA-Binding Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006736:Horses; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood* / Q000737:chemistry; D006868:Hydrolysis; D008969:Molecular Sequence Data; D011817:Rabbits; D012097:Repressor Proteins; D012697:Serine Endopeptidases / Q000097:blood* / Q000737:chemistry; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D004268:DNA-Binding Proteins; D018960:Hyaluronan Receptors; D012097:Repressor Proteins; D014157:Transcription Factors; D012697:Serine Endopeptidases