title: Adrenalin tolerance does not prevent bacterial translocation in a murine burn model.
abstract: Burn injury causes mesenteric vasoconstriction and bacterial translocation. Since catecholamines are powerful vasoconstrictors and elevated immediately after burn injury, we hypothesised that adrenaline tolerance might decrease burn-induced mesenteric vasoconstriction and bacterial translocation. Adrenaline tolerance was developed in Swiss albino mice. Adrenaline tolerant and control animals were subdivided into sham-burn and burn subgroups. 24 h after sham-burn or burn injury, specimens were obtained for microbiological evaluation. Also, in a separate group of adrenaline tolerant and control animals, superior mesenteric blood flow was measured. Burn injury increased bacterial translocation rate in both control (P = 0.001) and adrenaline tolerant groups (P = 0.0351). The caecal bacterial level increase was significant after burn injury in control groups (P = 0.0004) but was not significant in adrenaline tolerant animals (P = 0.743). Mesenteric blood flow was decreased significantly by burn injury in both control and adrenaline tolerant animals (P < 0.00001). The results showed that catecholamines do not mediate postburn mesenteric vasoconstriction or bacterial translocation.
authors.affiliation: Department of General Surgery, Faculty of Medicine, University of Hacettepe, Ankara, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Baykal
authors.forename: A B
authors.initials: AB
authors.lastname: Iskit
authors.forename: V
authors.initials: V
authors.lastname: Kaynaroglu
authors.forename: M O
authors.initials: MO
authors.lastname: Guc
authors.forename: G
authors.initials: G
authors.lastname: Hascelik
authors.forename: I
authors.initials: I
authors.lastname: Sayek
authors.forename: Y
authors.initials: Y
authors.lastname: Sanac
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D018988:Bacterial Translocation / Q000502:physiology*; D002056:Burns / Q000382:microbiology*; D002395:Catecholamines / Q000502:physiology; D002432:Cecum / Q000382:microbiology; D004361:Drug Tolerance; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D008198:Lymph Nodes / Q000382:microbiology; D008638:Mesenteric Arteries / Q000503:physiopathology*; D051379:Mice; D014661:Vasoconstriction / Q000502:physiology
chemical_list: D002395:Catecholamines; D004837:Epinephrine