Sensitive method for detection of human herpesviruses 6 and 7 in saliva collected in field studies.
To facilitate studies of the epidemiology and natural history of human herpesviruses 6 and 7 in infants, a practical method for collecting and quantifying the DNA of these viruses was developed. Saliva was collected using small strips of filter paper, and virus was detected using a real-time quantitative fluorescent-probe PCR assay. The sensitivity and specificity of this method even after prolonged drying of the specimens compared favorably to those of our traditional method of collecting and assaying saliva.
Zerr
D M
DM
Department of Pediatrics, University of Washington, Seattle, USA. zerr@u.washington.edu
Huang
M L
ML
Corey
L
L
Erickson
M
M
Parker
H L
HL
Frenkel
L M
LM
D004279:DNA, Viral / Q000032:analysis; D006566:Herpesviridae Infections / Q000453:epidemiology; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification*; D016199:Herpesvirus 7, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D017720:Molecular Epidemiology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D012463:Saliva / Q000821:virology*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods
D004279:DNA, Viral