beta-Endorphin binding and regulation of cytokine expression in Langerhans cells.
Neuropeptides and neurohormones have been shown to be able to regulate cutaneous immune reactions. Binding of beta-endorphin (beta-end) on epidermal Langerhans cells (LC) and effects of beta-end on cytokine expression were examined. Biotinylated beta-end bound to the mouse LC-like cell line, XS52, and the binding was replaced with intact beta-end but not with substance P. beta-End augmented secretion of IL-1 beta and IL-10 from XS52 cells were induced by a combination of LPS and GM-CSF. Induction of TNF alpha was suppressed by beta-end. The regulation of cytokine expression was confirmed in fresh LC by RT-PCR. These results suggest that beta-end is a regulator of skin immune function.
Hosoi
J
J
Shiseido Life Science Research Center, Yokohama, Japan. hosoi_junichi@po.shiseido.co.jp
Ozawa
H
H
Granstein
R D
RD
D000818:Animals; D019921:Biotinylation; D002460:Cell Line; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D007375:Interleukin-1 / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D007801:Langerhans Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011957:Receptors, Opioid / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D001615:beta-Endorphin / Q000378:metabolism* / Q000494:pharmacology*
D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011957:Receptors, Opioid; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D001615:beta-Endorphin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor