title: Effect of chelating agents on the molecular composition and extent of decalcification at cervical, middle and apical root dentin locations.
abstract: The aim of this study was to investigate the interaction of two chelating agents used in endodontic treatment with dentin from different root locations. Standardized cervical, middle and apical root dentin specimens were prepared and subjected to treatment with 15% neutral EDTA and RCPrep. Following rinsing with water, dentin surfaces were studied by reflected light optical microscopy and micro-MIR FTIR spectroscopy, while the wash-off extracts were analyzed by atomic absorption spectrometry (AAS) to determine the content of calcium and phosphorus. Neutral EDTA removed the smear layer and opened tubules while RCPrep did not. FTIR measurements showed higher decalcification of dentin surfaces after neutral EDTA treatment although its effect was reduced at apical regions. AAS measurements of calcium and phosphorus confirmed the higher dentin dissolution potential of EDTA at cervical and middle root dentin locations. The results of the present study implied different reaction modes for the two chelating agents tested which might affect the efficiency of the chemo-mechanical preparation when these agents are used.
authors.affiliation: Department of Endodontics, School of Dental and Oral Surgery, Columbia University, New York, USA.
authors.forename: K
authors.initials: K
authors.lastname: Verdelis
authors.forename: G
authors.initials: G
authors.lastname: Eliades
authors.forename: T
authors.initials: T
authors.lastname: Oviir
authors.forename: J
authors.initials: J
authors.lastname: Margelos
mesh_terms: D000704:Analysis of Variance; D002614:Chelating Agents / Q000494:pharmacology*; D003804:Dentin / Q000737:chemistry / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D015394:Molecular Structure; D010545:Peroxides / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013054:Spectrophotometry, Atomic; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D019227:Tooth Apex / Q000187:drug effects; D019237:Tooth Cervix / Q000187:drug effects; D017001:Tooth Demineralization / Q000139:chemically induced*; D014092:Tooth Root / Q000187:drug effects*; D014508:Urea / Q000494:pharmacology; D014885:Waxes / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D010545:Peroxides; D012388:Root Canal Irrigants; D014885:Waxes; C025081:RC-Prep; D014508:Urea; D004492:Edetic Acid