The static magnetic field effects on ouabain H3 binding by cancer tissue.
Radioactive labeled ouabain was used for estimating the static magnetic field (SMF) induced cell volume changes. Ouabain is a specific inhibitor of Na+/K+ ATPase, and can be used for estimating its quantity--thus giving information about the cell volume changes. Ouabain binding by cancer and normal glandular tissues of breast cancer patients and normal glandular tissues of healthy women was measured after exposure of tissues to SMF 0.2T. SMF exposure led to a decrease of ouabain binding in both normal and cancer tissues when ouabain concentration in the external medium was 10(-9) M, while in the case of higher concentrations of ouabain (10(-7) M, 10(-6) M) an increase of ouabain binding was seen. The normal glandular tissues of healthy women were sensitive to SMF only at the highest concentrations of ouabain used in our experiments. The SMF-induced decrease of binding at low ouabain concentrations was considered as an evidence for the dehydration effect of SMF. It is suggested that the SMF could influence the cancer cell metabolism through cell hydration changes.
Danielyan
A A
AA
Armenian Academy of Sciences, Biophysics Center, Yerevan, Armenia.
Mirakyan
M M
MM
Grigoryan
G Y
GY
Ayrapetyan
S N
SN
D001940:Breast / Q000378:metabolism* / Q000528:radiation effects; D001943:Breast Neoplasms / Q000378:metabolism*; D004574:Electromagnetic Fields; D005260:Female; D006801:Humans; D007700:Kinetics; D010042:Ouabain / Q000493:pharmacokinetics*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase