Multiplex PCRs for identification of Escherichia coli virulence genes.
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.
Pass
M A
MA
Department of Physiology and Pharmacology, The University of Queensland, St. Lucia, Queensland, 4072, Australia. mpass@usc.edu.au
Odedra
R
R
Batt
R M
RM
D000818:Animals; D017931:DNA Primers; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity*; D005243:Feces / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014774:Virulence / Q000235:genetics
D017931:DNA Primers