title: Endomorphins: localization, release and action on rat dorsal horn neurons.
abstract: Endomorphin (Endo) 1 and 2, two tetrapeptides isolated from the bovine and human brain, have been proposed to be the endogenous ligand for the mu-opiate receptor. A multi-disciplinary study was undertaken to address the issues of localization, release and biological action of Endo with respect to the rat dorsal horn. First, immunohistochemical studies showed that Endo-1- or Endo-2-like immunoreactivity (Endo-1- or Endo-2-LI) is selectively expressed in fiber-like elements occupying the superficial layers of the rat dorsal horn, which also exhibit a high level of mu-opiate receptor immunoreactivity. Second, release of immunoreactive Endo-2-like substances (irEndo) from the in vitro rat spinal cords upon electrical stimulation of dorsal root afferent fibers was detected by the immobilized antibody microprobe technique. The site of release corresponded to laminae I and II where the highest density of Endo-2-LI fibers was localized. Lastly, whole-cell patch clamp recordings from substantia gelatinosa (SG) neurons of rat lumbar spinal cord slices revealed two distinct actions of exogenous Endo-1 and Endo-2: (1) depression of excitatory and/or inhibitory postsynaptic potentials evoked by stimulation of dorsal root entry zone, and (2) hyperpolarization of SG neurons. These two effects were prevented by the selective mu-opiate receptor antagonist beta-funaltrexamine. The localization of endomorphin-positive fibers in superficial layers of the dorsal horn and the release of irEndo upon stimulation of dorsal root afferents together with the observation that Endo inhibits the activity of SG neurons by interacting with mu-opiate receptors provide additional support of a role of Endo as the endogenous ligand for the mu-opiate receptor in the rat dorsal horn.
authors.affiliation: Department of Pharmacology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tenn. 37614, USA. dunnae@etsu.edu
authors.forename: N J
authors.initials: NJ
authors.lastname: Dun
authors.forename: S L
authors.initials: SL
authors.lastname: Dun
authors.forename: S Y
authors.initials: SY
authors.lastname: Wu
authors.forename: C A
authors.initials: CA
authors.lastname: Williams
authors.forename: E H
authors.initials: EH
authors.lastname: Kwok
mesh_terms: D000344:Afferent Pathways / Q000502:physiology; D000701:Analgesics, Opioid / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D004558:Electric Stimulation; D005260:Female; D007150:Immunohistochemistry; D008024:Ligands; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009842:Oligopeptides / Q000276:immunology / Q000378:metabolism*; D020671:Posterior Horn Cells / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D017450:Receptors, Opioid, mu / Q000819:agonists / Q000276:immunology; D013116:Spinal Cord / Q000737:chemistry; D013126:Spinal Nerve Roots / Q000502:physiology; D013376:Substantia Gelatinosa / Q000187:drug effects; D014018:Tissue Distribution
chemical_list: D000701:Analgesics, Opioid; D000906:Antibodies; D008024:Ligands; D009842:Oligopeptides; D017450:Receptors, Opioid, mu; C105293:endomorphin 1; C105294:endomorphin 2