title: Haemopoietic biglycan produced by brain cells stimulates growth of microglial cells.
abstract: We have recently found that soluble biglycan purified from rat thymic myoid cells had haemopoietic activity capable of inducing preferential growth and differentiation of monocytic lineage cells from various haemopoietic sources, including brain microglial cells. In the present study, to understand developmental mechanisms of microglial/monocytic cells in the brain, we have attempted to identify haemopoietic activity of the brain biglycan. The mRNA and the immunological epitope of biglycan were detected in the rat brain homogenates and several rat glial cell lines. Immunohistochemical study showed that several different types of brain cells produced biglycan. During development biglycan synthesis in the brain appeared to be increased. The brain haemopoietic biglycan was easily separated by DEAE-Sepharose chromatography from the macrophage colony stimulating factor (M-CSF) which was concomitantly produced from the brain cells. The brain haemopoietic biglycan, purified through immunoaffinity column, indeed stimulated growth of primarily cultured microglial cells. Taken together, these results suggest that the haemopoietic biglycan plays an important role in generating brain-specific circumstances for development of microglial/monocytic cells.
authors.affiliation: National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi-cho, Kodaira, Tokyo, Japan. kikuchi@ncnp.go.jp
authors.forename: A
authors.initials: A
authors.lastname: Kikuchi
authors.forename: H
authors.initials: H
authors.lastname: Tomoyasu
authors.forename: I
authors.initials: I
authors.lastname: Kido
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: I
authors.initials: I
authors.lastname: Nonaka
authors.forename: N
authors.initials: N
authors.lastname: Iwakami
authors.forename: I
authors.initials: I
authors.lastname: Kamo
mesh_terms: D000818:Animals; D058578:Biglycan; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D016326:Extracellular Matrix Proteins; D006410:Hematopoiesis / Q000502:physiology*; D007150:Immunohistochemistry; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D017628:Microglia / Q000166:cytology*; D008970:Molecular Weight; D009474:Neurons / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: C546886:Bgn protein, rat; D058578:Biglycan; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor