[The synthesis of a cobalt(II) tetracarboxyphthalocyanine- deoxyribooligonucleotide conjugate as a reagent for the directed DNA modification].
The cobalt(II) tetracarboxyphthalocyanine-deoxyribonucleotide pd(TCTTCCCA) conjugate was synthesized. The phthalocyanine N-succinimide ester prepared from phthalocyanine using DCC was mixed in DMF with an aqueous solution of the oligonucleotide bearing a 1,3-diaminopropane linker at the 5'-phosphate. The resulting conjugate was tested in the intraduplex reaction with target 14-mer and 22-mer oligonucleotides containing conjugate-complementary sequences. In the presence of O2 and a thiol (2-mercaptoethanol or DTT) as a coupled reducer or H2O2, sequence-specific DNA modification was observed that caused the cleavage of the target upon treatment with piperidine.
Koval'
V V
VV
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Russia.
Chernonosov
A A
AA
Abramova
T V
TV
Ivanova
T M
TM
Fedorova
O S
OS
Knorre
D G
DG
D000818:Animals; D003035:Cobalt; D004247:DNA / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007211:Indoles / Q000138:chemical synthesis* / Q000737:chemistry*; D054833:Isoindoles; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*
D007211:Indoles; D054833:Isoindoles; D009841:Oligonucleotides; D003035:Cobalt; D004247:DNA; C013647:phthalocyanine