title: Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance.
abstract: The line probe assay (LiPA), a rapid molecular method for detecting rifampicin resistance (RMPr) in Mycobacterium tuberculosis, correctly identified all 145 rifampicin-sensitive (RMPs) and 262 (98.5%) of 266 RMPr strains among 411 isolates collected from diverse countries. If used as a marker of multidrug-resistant tuberculosis (MDR-TB), detection of RMPr by LiPA would have detected 236 of the 240 MDR strains in this study but would have wrongly suggested the presence of MDR in 26 RMP-monoresistant isolates (sensitivity 98.3%, specificity 84.8%). Hence, the reliability of using LiPA (or any other rapid RMPr-detection method) as a surrogate marker of MDR-TB largely depends on the prevalence of RMP-monoresistance in the study population. This approach must therefore be validated in each local situation.
authors.affiliation: Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium. traore@itg.be
authors.forename: H
authors.initials: H
authors.lastname: Traore
authors.forename: K
authors.initials: K
authors.lastname: Fissette
authors.forename: I
authors.initials: I
authors.lastname: Bastian
authors.forename: M
authors.initials: M
authors.lastname: Devleeschouwer
authors.forename: F
authors.initials: F
authors.lastname: Portaels
mesh_terms: D000349:Africa / Q000453:epidemiology; D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001208:Asia / Q000453:epidemiology; D004351:Drug Resistance; D005060:Europe / Q000453:epidemiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D015336:Molecular Probe Techniques; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013020:South America / Q000453:epidemiology; D013045:Species Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000904:Antibiotics, Antitubercular; D012293:Rifampin