title: Different deoxyribonucleases in human lymphocytes.
abstract: The distribution pattern of deoxyribonuclease activities in human lymphocytes has been examined by micro-disc-electrophoresis. Four groups of deoxyribonuclease activities, differing in their electrophoretic mobility, in the nature of their optimal substrate and in their optimal incubation conditions, are characterized. There are two alkaline DNase-activities. One corresponds to DNase I (EC 3.1.4.5), the other having pH optimum of about pH 9.0, prefers denatured DNA as substrate and is not dependent on divalent cations. The fractions with an acid pH optimum can be subdivided into two groups, which differ in their activity towards native DNA, towards denatured DNA, in their activity when succinate is present and in their pH optimum.
authors.forename: E J
authors.initials: EJ
authors.lastname: Zöllner
authors.forename: W
authors.initials: W
authors.lastname: Helm
authors.forename: R K
authors.initials: RK
authors.lastname: Zahn
authors.forename: J
authors.initials: J
authors.lastname: Beck
authors.forename: M
authors.initials: M
authors.lastname: Reitz
mesh_terms: D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male
chemical_list: D003851:Deoxyribonucleases