Regulation and manipulation of the gene clusters encoding type-I PKSs.
Modular polyketide synthases are large, multifunctional enzyme complexes that are involved in the biosynthesis of important polyketides. Recent studies have revolutionized our understanding of the linear organization of polyketide-synthase-gene clusters. They have provided crucial information on the initiation, elongation and termination of polyketide chains, and thus a rational basis for the generation of novel compounds. Combinatorial libraries have helped this field to move from a random approach to a more empirical phase. The large number of diverse analogs of antibiotics that are presently produced demonstrate the enormous potential of combinatorial biosynthesis.
Lal
R
R
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India. duzdel@del2.vsnl.net.in
Kumari
R
R
Kaur
H
H
Khanna
R
R
Dhingra
N
N
Tuteja
D
D
D020650:Combinatorial Chemistry Techniques; D004917:Erythromycin / Q000378:metabolism; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D005810:Multigene Family; D012294:Rifamycins / Q000378:metabolism; D020123:Sirolimus / Q000378:metabolism
D009097:Multienzyme Complexes; D012294:Rifamycins; D004917:Erythromycin; D020123:Sirolimus