Expression of T-cell cytokines in challenged skin of murine allergic contact photosensitivity: low responsiveness is associated with induction of Th2 cytokines.
Murine contact photosensitivity (CPS) to 3,3',4', 5-tetrachlorosalicylanilide (TCSA) is genetically controlled mainly by the major histocompatibility complex. The H-2(b,d) haplotypes are closely associated with high responders, whereas mice with H-2(k) are low or non-responders. We found that BALB/c (H-2(d)) mice were high responders in CPS not only to TCSA but also to chlorpromazine (CPZ) and benzocaine, whereas AKR/n (H-2(k)) mice were hyporesponsive to these three photoallergic agents. To elucidate the relationship between CPS responsiveness and T helper cell induction, the expression of T-cell cytokines was examined by reverse transcriptase polymerase chain reaction in the elicited skin of CPS to the three chemicals. The expression levels of interleukin(IL)-2 and interferon-gamma mRNAs were markedly higer in BALB/c mice than AKR/n mice, whereas mRNA for IL-4 was expressed strongly in AKR/n mice. These data suggest that the hyporesponsiveness of CPS to the photoallergens in AKR/n mice is closely associated with the activation/induction of Th2 cytokines at the challenged sites.
Suzuki
K
K
Kanebo Ltd., Cosmetics Laboratory, 3-28 5-Chome Kotobuki-cho, Odawara, Japan. k-suzuki@oda.cos.kanebo.co.jp
Yamazaki
S
S
Tokura
Y
Y
D000485:Allergens / Q000494:pharmacology; D000818:Animals; D001566:Benzocaine / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D016207:Cytokines / Q000235:genetics*; D003877:Dermatitis, Contact / Q000276:immunology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012458:Salicylanilides / Q000494:pharmacology; D012867:Skin / Q000276:immunology*; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
D000485:Allergens; D016207:Cytokines; D007376:Interleukin-2; D012458:Salicylanilides; D015847:Interleukin-4; D007371:Interferon-gamma; C003390:3,3',4',5-tetrachlorosalicylanilide; D001566:Benzocaine; D002746:Chlorpromazine