title: Analysis of the human KCNH2(HERG) gene: identification and characterization of a novel mutation Y667X associated with long QT syndrome and a non-pathological 9 bp insertion.
abstract: Long QT (LQT) syndrome is a potentially life-threatening disorder, characterized by a distinct cardiac arrhythmia known as torsades de pointes. Mutations within a number of genes linked to the familial form, including that coding for a cardiac potassium channel called KCNH2 (HERG), have been described based on the characterized genomic organization. A standardized method was developed to screen the entire gene for gene variants. We report a single base pair substitution, introducing a premature STOP codon at codon 667 of the gene in a healthy individual with an extended QTc interval (460 msec). In vitro expression of the codon Y667X variant in Xenopus oocyte suggests that the autosomal dominant variant does not function in a dominant/negative manner and cannot co-assemble to form a channel, resulting in a reduction of the KCNH2 current, and an extension of the QT interval. This indicates that pathogenic LQT gene variants exist in the apparently normal population, the prognosis and clinical consequences of which remain to be determined. The assays described should facilitate future studies into this area.
authors.affiliation: Department of Pharmacogenomics, Janssen Research Foundation, Janssen Pharmaceutica, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Paulussen
authors.forename: P
authors.initials: P
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Pangalos
authors.forename: P
authors.initials: P
authors.lastname: Verhasselt
authors.forename: R
authors.initials: R
authors.lastname: Marrannes
authors.forename: C
authors.initials: C
authors.lastname: Verfaille
authors.forename: I
authors.initials: I
authors.lastname: Vandenberk
authors.forename: R
authors.initials: R
authors.lastname: Crabbe
authors.forename: F
authors.initials: F
authors.lastname: Konings
authors.forename: W
authors.initials: W
authors.lastname: Luyten
authors.forename: M
authors.initials: M
authors.lastname: Armstrong
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins; D003062:Codon / Q000235:genetics; D004268:DNA-Binding Proteins; D000072237:ERG1 Potassium Channel; D004594:Electrophysiology; D051638:Ether-A-Go-Go Potassium Channels; D006801:Humans; D008133:Long QT Syndrome / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000235:genetics*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000235:genetics* / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG; D014982:Xenopus laevis / Q000235:genetics
chemical_list: D027682:Cation Transport Proteins; D003062:Codon; D004268:DNA-Binding Proteins; C072312:ERG protein, human; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; C000606913:KCNH2 protein, human; C092509:KCNH6 protein, human; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG