title: Serum amyloid A-derived peptides, present in human rheumatic synovial fluids, induce the secretion of interferon-gamma by human CD(4)(+) T-lymphocytes.
abstract: Serum amyloid A (SAA) is a major acute-phase protein whose biochemical functions remain largely obscure. Human rheumatic synovial fluids were screened by high performance liquid chromatography mass spectrometry for SAA-derived peptides, specifically the sequence AGLPEKY (SAA(98-104)) which was previously shown to modulate various leukocyte functions. Two such fluids were found to contain a truncated version of SAA(98-104). Synthetic SAA(98-104) and several of its analogs were shown capable of binding isolated human CD(4)(+) T-lymphocytes and stimulating them to produce interferon-gamma. Given the high acute-phase serum level of SAA and its massive proteolysis by inflammatory related enzymes, SAA-derived peptides may be involved in host defense mechanisms.
authors.affiliation: Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel.
authors.forename: E J
authors.initials: EJ
authors.lastname: Yavin
authors.forename: L
authors.initials: L
authors.lastname: Preciado-Patt
authors.forename: O
authors.initials: O
authors.lastname: Rosen
authors.forename: M
authors.initials: M
authors.lastname: Yaron
authors.forename: R D
authors.initials: RD
authors.lastname: Suessmuth
authors.forename: D
authors.initials: D
authors.lastname: Levartowsky
authors.forename: G
authors.initials: G
authors.lastname: Jung
authors.forename: O
authors.initials: O
authors.lastname: Lider
authors.forename: M
authors.initials: M
authors.lastname: Fridkin
mesh_terms: D001053:Apolipoproteins / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D000685:Serum Amyloid A Protein / Q000276:immunology*; D013582:Synovial Fluid / Q000276:immunology
chemical_list: D001053:Apolipoproteins; D010455:Peptides; D000685:Serum Amyloid A Protein; D007371:Interferon-gamma