A study on the surface hydrophobicity of lipases.
Surface properties, including surface net and local hydrophobicities, of bovine serum albumin, gamma-globulin, and six lipases of different origins were evaluated using the aqueous two-phase partitioning method. Each showed a specific and characteristic pattern of surface properties. Correlations between the protein surface hydrophobicities and the coverages of the proteins by lipid-coating with a synthetic detergent, dioleyl glucosyl L-glutamate, were discussed. The results indicated that the surface net hydrophobicity of each protein was indicative of the affinity of the protein for the coating detergent applied in lipid-coating.
Sugimura
Y
General Education Division, Oshima National College of Maritime Technology, Komatsu, Oshima-cho, Yamaguchi, Japan
Fukunaga
K
Matsuno
T
Nakao
K
Goto
M
Nakashio
F