Effect of trehalose and EDTA on cryoprotective action of ram semen diluents.
To obtain better ram semen extenders for artificial insemination (AI), we developed effective trehalose-containing hypertonic diluents. The cryoprotective action of trehalose has been explained by its dehydrating activity and interaction with cell membranes. Accordingly, we tested the cryopreserving capacity of different combinations of a Salamon's modified plus trehalose extender with EDTA. Evaluations were based on the percentage of motile spermatozoa and acrosome integrity, measured after thawing and after a 4-h post-thaw resistance test at 37 degrees C. We conclude that the combination of trehalose plus EDTA confers the highest cryopreserving activity tested, not only for freeze-thawing but also for post-thawing resistance, possibly by removing calcium from the medium thereby preventing cation competition with trehalose for membrane-binding sites.
Aisen
E G
EG
Laboratorio de Teriogenología, Facultad de Ciencias Agrarias, Universidad Nacional del Comahue, Río Negro, Argentina. eaisen@uncoma.edu.ar
Alvarez
H L
HL
Venturino
A
A
Garde
J J
JJ
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D015925:Cryopreservation / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D012661:Semen / Q000187:drug effects*; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects; D014199:Trehalose / Q000494:pharmacology*
D002614:Chelating Agents; D003451:Cryoprotective Agents; D008563:Membrane Lipids; D004492:Edetic Acid; D014199:Trehalose; D002118:Calcium