ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci.
In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from Taenia crassiceps vesicular fluid (VF-Tcra) and crude larvae extract (T-Tcra), and from Taenia solium extracts of scolex (S-Ts) and of larvae (T-Ts). A checkerboard evaluation of antigen, serum and conjugate dilutions, as well as the use of Tween-20 and skim cow milk in wash and blocking solution had a marked effect on improving ELISA performance. All the antigens showed a good performance, but VF-Tcra was the best, with 96.0% and 80.0% sensitivities for cut-offs respectively at 2sd and 3sd, and corresponding specificities of 97.5% and 100.0%. Cross-reactivity was observed only with hydatidosis and ascaridiosis. In view of the high performance observed, the ELISA test should be recommended for the diagnosis of cysticercosis in suspected swine in slaughterhouses and for the screening of cysticercosis in swine production. These results will support integrated measures of cysticercosis control throughout the chain of swine production, effectively contributing to public health.
Pinto
P S
PS
Department of Veterinary, Viçosa Federal University, Viçosa, MG, 36571-000, Brazil. pintopsa@mail.ufv.br
Vaz
A J
AJ
Germano
P M
PM
Nakamura
P M
PM
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood / Q000302:isolation & purification*; D015415:Biomarkers / Q000097:blood; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000947:Antigens, Helminth; D015415:Biomarkers