Mechanisms of fidelity in pre-mRNA splicing.
The pre-mRNA splicing machinery consists of five small nuclear RNAs (U1, U2, U4, U5 and U6) and more than fifty proteins. Over the past year, important advances have been made in understanding how these factors function to achieve fidelity in splicing. Of particular note were the discoveries that the splicing factor U2AF(35) recognizes the AG dinucleotide at the 3' splice site early in spliceosome assembly, that a DEAD-box ATPase, Prp28, triggers specific rearrangements of the spliceosome, and that the splicing factor hSlu7 functions in the fidelity of AG choice during catalytic step II of splicing.
Reed
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Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. rreed@hms.harvard.edu
D000818:Animals; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008954:Models, Biological; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D017381:Spliceosomes / Q000378:metabolism
D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012322:RNA Precursors; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins