title: Antioxidant properties of pyrrolidine dithiocarbamate and its protection against Cr(VI)-induced DNA strand breakage.
abstract: Pyrrolidine dithiocarbamate (PDTC) is considered an antioxidant and is frequently used to study the role of free radical reactions in various biological processes and against free radical-induced cellular injuries. However, its antioxidant properties are not characterized. In this study, electron spin resonance (ESR) was used to investigate the antioxidant potential of PDTC with hydroxyl radical (*OH) and superoxide anion radicals (O2*-). The Fenton reaction [Fe(II) + H2O2 --> Fe(II) + *OH + OH-)] and xanthine and xanthine oxidase were used as sources of *OH and O2*- radicals, respectively. The results show that PDTC effectively scavenges *OH radicals with a reaction rate constant of approximately 2.73 x 10(10) M(-1) s(-1), which is comparable to other efficient *OH radical scavengers, such as ascorbate and glutathione. PDTC is also able to scavenge O2*- radicals. Through its antioxidant properties, PDTC protects against Cr(VI)-induced DNA strand breakage.
authors.affiliation: Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia, USA. xas0@cdc.gov
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.forename: S S
authors.initials: SS
authors.lastname: Leonard
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Ding
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002857:Chromium / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D011759:Pyrrolidines / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D013481:Superoxides / Q000737:chemistry / Q000378:metabolism; D013859:Thiocarbamates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D011759:Pyrrolidines; D013859:Thiocarbamates; D002857:Chromium; D013481:Superoxides; C020972:pyrrolidine dithiocarbamic acid; D017665:Hydroxyl Radical