title: Identification of androgen, estrogen and progesterone receptor mRNAs in the eye.
abstract: OBJECTIVE
  Previous research has demonstrated that sex steroids exert a significant influence on the structure and function of numerous ocular tissues. To begin to explore the underlying basis of this hormone action, we examined whether various anterior and posterior tissues of the eye contain androgen, estrogen and progesterone receptor mRNAs.
  
  
  METHODS
  Tissue samples were obtained from adult male and female rats, rabbits and humans, processed for the isolation of total RNA and analyzed by RT-PCR, agarose gel electrophoresis and Southern blot hybridization. All PCR amplifications included positive and negative controls.
  
  
  RESULTS
  Our findings showed that androgen, estrogen and/or progesterone receptor mRNAs are present in the lacrimal gland, lacrimal gland acinar epithelial cells, meibomian gland, lid, palpebral and bulbar conjunctivae, cornea, iris/ciliary body, lens, retina/uvea, retina/choroid and retinal pigment epithelial cells of rats, rabbits or humans.
  
  
  CONCLUSIONS
  Our findings demonstrate that sex steroid receptor mRNAs exist in a variety of ocular tissues and suggest that these sites may represent target organs for androgens, estrogens and/or progestins.
authors.affiliation: Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Wickham
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: I
authors.initials: I
authors.lastname: Toda
authors.forename: E M
authors.initials: EM
authors.lastname: Rocha
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: D A
authors.initials: DA
authors.lastname: Sullivan
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005123:Eye / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D011944:Receptors, Androgen / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*; D011980:Receptors, Progesterone / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone