Cloning and Expression of an Elongation Factor-1alpha in Sea Bream (Sparus aurata) Larvae and Adult Tissue.
A clone encoding the polypeptide elongation factor EF-1alpha was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1alpha in other animal species. EF-1alpha messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1alpha appeared to have a relatively uniform distribution across all the tissues analyzed.
Nowell
MA
Cardiff School of Biosciences, Cardiff University, Museum Avenue, P.O. Box 911, Cardiff CF1 3US, Wales, U.K.
Power
DM
Guerreiro
PM
Llewellyn
L
Ramsurn
V
Wigham
T
Sweeney
GE