title: Delayed graft rejection in pre-vascularised corneas after subconjunctival injection of clodronate liposomes.
abstract: OBJECTIVE
  To test the effects of clodronate liposomes on graft survival and neovascularisation after transplantation in pre-vascularised recipient corneas.
  
  
  METHODS
  Corneal neovascularisation was induced in F344 rats by injecting heat inactivated rabbit serum intrastromally. After 4 weeks F344 rats were orthotopically grafted with corneal buttons from DA rats. Directly after transplantation and on 2, 4, 6 and 8 days postoperatively clodronate liposomes were administrated subconjunctivally in one group, whereas the other group remained untreated. For 60 days grafts were observed for signs of graft rejection and neovascularisation.
  
  
  RESULTS
  Graft survival was significantly prolonged, but not prevented in clodronate liposome treated rats compared to untreated rats ( p =.004). Also clodronate liposome administration delays growth of corneal neovascularisation after transplantation.
  
  
  CONCLUSIONS
  Previous studies revealed that clodronate liposomes prevent corneal graft rejection and reduce neovascularisation in orthotopic corneal allotransplantation in rats. This study shows that also in pre-vascularised recipient corneas subconjunctival administration of clodronate liposomes seems to delay corneal graft rejection and reduces neovascularisation.
authors.affiliation: Department of Ophthalmology, Groningen University Hospital, Groningen, The Netherlands. t.slegers@ohk.azg.nl
authors.forename: T P
authors.initials: TP
authors.lastname: Slegers
authors.forename: N
authors.initials: N
authors.lastname: van Rooijen
authors.forename: G
authors.initials: G
authors.lastname: van Rij
authors.forename: R
authors.initials: R
authors.lastname: van der Gaag
mesh_terms: D000818:Animals; D004002:Clodronic Acid / Q000008:administration & dosage* / Q000627:therapeutic use; D003228:Conjunctiva; D003315:Cornea / Q000098:blood supply*; D016039:Corneal Transplantation; D004337:Drug Carriers; D006084:Graft Rejection / Q000517:prevention & control*; D007267:Injections; D008081:Liposomes; D008297:Male; D009389:Neovascularization, Pathologic / Q000209:etiology / Q000517:prevention & control; D011817:Rabbits / Q000097:blood; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D004002:Clodronic Acid