Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks.
Amplified fragment length polymorphism (AFLP) markers with a total of 256 EcoRI + ANN - MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 fragments per primer combination were amplified, ranging from a maximum of 226 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT - M + CNN produced very few distinct fragments. By using 15 randomly chosen EcoRI + ANN - MseI + CNN primer combinations, 928 AFLP markers were allocated to wheat chromosomes, of which 131 were assigned to specific chromosome arms. These AFLP markers were locus-specific and randomly distributed on the different chromosomes. In addition, 6 and 41 AFLP markers were simultaneously absent in two nulli-tetrasomics (NTs) of both homoeologous and non-homoeologous groups, respectively, whereas additional fragments were detected in N1BT1A, N5AT5D, and N6BT6A lines.
Huang
X
X
Technische Universität München, Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, Freising-Weihenstephan, Germany.
Zeller
F J
FJ
Hsam
S L
SL
Wenzel
G
G
Mohler
V
V
D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019655:Quantitative Trait, Heritable; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers