Studies on hepatic mitochondrial cytochrome P-450 during Plasmodium yoelii infection and pyrimethamine treatment in mice.
Hepatic mitochondrial cytochrome P-450 and b(5) activities were significantly depressed, whereas heme and hemozoin were increased during Plasmodium yoelii infection. Type II, aniline-HCl binding efficacy and polyacrylamide gel electrophoretic profile also depicted impairment of cytochrome P-450 during infection. However, the above alterations were more pronounced in the infected hepatic mitochondria, compared to microsomes. Oral treatment of pyrimethamine (10 mg/kg body weightx4 days) to P. yoelii-infected mice brought the altered levels of mitochondrial and microsomal cytochrome P-450 and b(5) to almost normal, whereas heme and hemozoin levels remained unchanged.
Srivastava
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P
Division of Pharmacokinetics and Metabolism, Central Drug Research Institute, Lucknow 1, India.
Pandey
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VC
D000818:Animals; D000962:Antimalarials / Q000627:therapeutic use*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008288:Malaria / Q000188:drug therapy / Q000201:enzymology*; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D010967:Plasmodium yoelii; D011739:Pyrimethamine / Q000627:therapeutic use*
D000962:Antimalarials; D003577:Cytochrome P-450 Enzyme System; D011739:Pyrimethamine