title: The binding of amino acids to the herbicide 2,4-dichlorophenoxy acetic acid.
abstract: The interaction of amino acids with the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) was studied by charge-transfer chromatography carried out on diatomaceous layers covered with different amount of 2,4-D and the effect of salts on the strength of interaction was elucidated. It was established that Arg, His, Lys, Orn, Phe and Trp binds to 2,4-D, the binding process is of saturation character. Principal component analysis proved that the concentration of 2,4-D exerts the highest impact on the interaction and the effect of salts is of secondary importance. The results suggest that these amino acid residues may account for the binding of 2,4-D to proteins and can play a considerable role in the detoxification processes by forming conjugates with 2,4-D.
authors.affiliation: Institute of Chemistry, Chemical Research Center, Hungarian Academy of Sciences, Budapest.
authors.forename: E
authors.initials: E
authors.lastname: Forgács
authors.forename: T
authors.initials: T
authors.lastname: Cserháti
authors.forename: I
authors.initials: I
authors.lastname: Barta
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D016000:Cluster Analysis; D004305:Dose-Response Relationship, Drug; D006540:Herbicides / Q000378:metabolism*; D015636:Magnesium Chloride / Q000378:metabolism; D015233:Models, Statistical; D008962:Models, Theoretical; D011189:Potassium Chloride / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D000596:Amino Acids; D006540:Herbicides; D015636:Magnesium Chloride; D015084:2,4-Dichlorophenoxyacetic Acid; D012965:Sodium Chloride; D011189:Potassium Chloride