Human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50 are admixtures of the human colon carcinoma cell line HCT 116.
In two recently described human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50, derived from one tumour, we detected identical E-cadherin and beta-catenin gene mutations as in colon carcinoma cell line HCT 116. We demonstrate by HLA-typing, mutation analysis and microsatellite analysis that cell lines JROECL 47 and JROECL 50 are admixtures of the human colon adenocarcinoma cell line HCT 116.
Wijnhoven
B P
BP
Erasmus University Medical Centre Rotterdam, Department of Surgery, University Hospital Dijkzigt, The Netherlands.
Tilanus
M G
MG
Morris
A G
AG
Darnton
S J
SJ
Tilanus
H W
HW
Dinjens
W N
WN
D000230:Adenocarcinoma / Q000473:pathology*; D001483:Base Sequence; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm