Interactions of inflammatory mediators and low pH not influenced by capsazepine in rat cutaneous nociceptors.
The rat skin-saphenous nerve preparation was used to record from mechano-heat sensitive C-fibers whose receptive fields were superfused with various solutions of low pH and of bradykinin, serotonin and prostaglandin E2. Only synchronous application of protons and mediators resulted in a significant nearly three-fold augmentation of the nociceptive pH response, and capsazepine (10(-5) M) did not block this short-lived enhancement. Thus, it does not seem to involve the capsaicin receptor (VRI) which is in contrast to a previous finding from cultured sensory neurons.
Habelt
C
C
Institute of Physiology and Experimental Pathophysiology, Erlangen, Germany.
Kessler
F
F
Distler
C
C
Kress
M
M
Reeh
P W
PW
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D004347:Drug Interactions / Q000502:physiology*; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007249:Inflammation / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology*; D018836:Inflammation Mediators / Q000378:metabolism* / Q000494:pharmacology*; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects* / Q000378:metabolism*; D010120:Oxytocics / Q000494:pharmacology; D011522:Protons / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D012867:Skin / Q000187:drug effects* / Q000294:innervation* / Q000503:physiopathology
D018836:Inflammation Mediators; D010120:Oxytocics; D011522:Protons; D012701:Serotonin; D015232:Dinoprostone; C071423:capsazepine; D002211:Capsaicin; D001920:Bradykinin