title: [Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2'- deoxyuridine-containing oligodeoxynucleotides].
abstract: Novel reagents for the fluorescent labeling of oligo- and polynucleotides have been prepared: 5-(1-pyrenylethynyl)-2'-deoxyuridine 3'-phosphoramidite and a solid support carrying this nucleoside. Oligonucleotides containing one or several modified units have been synthesized, and the fluorescence of these probes has been shown to change upon hybridization with the complementary sequence.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Malakhov
authors.forename: E V
authors.initials: EV
authors.lastname: Malakhova
authors.forename: S V
authors.initials: SV
authors.lastname: Kyznitsova
authors.forename: I V
authors.initials: IV
authors.lastname: Grechishnikova
authors.forename: I A
authors.initials: IA
authors.lastname: Prokhorenko
authors.forename: M V
authors.initials: MV
authors.lastname: Skorobogatyĭ
authors.forename: V A
authors.initials: VA
authors.lastname: Korshun
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Berlin
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: C410922:5-(pyrenylethynyl)-2'-deoxyuridine; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D011721:Pyrenes; D014529:Uridine