title: Effects of repeated stress on expression of interleukin-6 (IL-6) and IL-6 receptor mRNAs in rat hypothalamus and midbrain.
abstract: We examined the effects of single and repeated stress on the expression of interleukin-6 (IL-6) and IL-6 receptor (IL-6R) mRNAs in the rat midbrain and hypothalamus using reverse transcriptase-polymerase chain reaction (RT-PCR). Following a single episode of restraint stress for 4 hours (1R) or 4 hours per day on two (2R) or three (3R) consecutive days, the hypothalamus and midbrain were removed immediately and the levels of IL-6 and IL-6R mRNAs in both regions were determined. Regional differences in stress-related changes in mRNA levels were noted. The expression of IL-6 mRNA in the hypothalamus did not change in 1R group but decreased in 2R and 3R groups. The expression of IL-6R mRNA in the same region significantly diminished in all groups. In the midbrain, the expression of IL-6 mRNA increased in 1R group and decreased in 2R and 3R, while the expression of IL-6R mRNA significantly diminished in 1R and 3R groups but was not different from control in 2R group. Our findings indicate that repeated stress in rats produce changes in IL-6 and IL-6R mRNAs in the midbrain and hypothalamus that are different than those of a single stress episode.
authors.affiliation: Department of Psychiatry, Mie University School of Medicine, Tsu, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Miyahara
authors.forename: T
authors.initials: T
authors.lastname: Komori
authors.forename: R
authors.initials: R
authors.lastname: Fujiwara
authors.forename: K
authors.initials: K
authors.lastname: Shizuya
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Ohmori
authors.forename: Y
authors.initials: Y
authors.lastname: Okazaki
mesh_terms: D000818:Animals; D007031:Hypothalamus / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008636:Mesencephalon / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D019947:Receptors, Interleukin-6 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013312:Stress, Physiological / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger; D019947:Receptors, Interleukin-6