[Cytotoxic and mitogenic effect of antimicrobial peptides from neutrophils on cultured cells].
Cytotoxic and mitogenic activities of human and rabbit defensines (HNP and NP-2, resp.) and pig antimicrobial peptides from leukocytes (PR-39, prophenin PF-2 and protegrin PG-2) were studied. The above peptides were added to serum-free cell culture medium of the target cell lines K562, L929 and Hep22a. Cytotoxicity was estimated within 1, 3, 6, 24 and 48 h of cell incubation with the tested peptides in concentrations 1, 10, 25 or 100 micrograms/ml. All the examined peptides exhibited a distinct time- and concentration-dependent cytotoxicity. Moreover, by contrast to pig peptides, defensines could induce proliferation in cell subpopulations from cell lines L929 amd Hep22a, or L929 (defensines HNP and NP-2, resp.), keeping resistance to their cytotoxic action.
Pleskach
V A
VA
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Aleshina
G M
GM
Artsybasheva
I V
IV
Shamova
O V
OV
Kozhukharova
I V
IV
Goĭlo
T A
TA
Kokriakov
V N
VN
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D023082:Defensins; D006801:Humans; D008934:Mitogens / Q000494:pharmacology*; D009504:Neutrophils / Q000737:chemistry*; D011506:Proteins / Q000494:pharmacology*; D011817:Rabbits
D000900:Anti-Bacterial Agents; D016895:Culture Media, Serum-Free; D023082:Defensins; D008934:Mitogens; D011506:Proteins