title: SV40 can be reproducibly detected in paraffin-embedded mesothelioma samples.
abstract: We studied tissue sections from 18 paraffin embedded mesothelioma specimens diagnosed by the Pathology Department of S. Chiara Hospital of Pisa. Using PCR analysis and Southern blot hybridization we examined the specimens for the DNA regulatory region of the virus. 10/18 (55.5%) of the samples tested contained SV40 DNA regulatory sequences, and of these positive samples, 80% were found to contain Tag sequences by PCR and Southern Blot hybridization. These results confirm that SV40 can be amplified and detected in paraffin embedded mesothelioma samples.
authors.affiliation: Department of Endocrinology and Metabolism, University of Pisa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Cristaudo
authors.forename: A
authors.initials: A
authors.lastname: Powers
authors.forename: A
authors.initials: A
authors.lastname: Vivaldi
authors.forename: R
authors.initials: R
authors.lastname: Foddis
authors.forename: G
authors.initials: G
authors.lastname: Guglielmi
authors.forename: V
authors.initials: V
authors.lastname: Gattini
authors.forename: R
authors.initials: R
authors.lastname: Buselli
authors.forename: G
authors.initials: G
authors.lastname: Sensales
authors.forename: E
authors.initials: E
authors.lastname: Ciancia
authors.forename: F
authors.initials: F
authors.lastname: Ottenga
mesh_terms: D000328:Adult; D000368:Aged; D015139:Blotting, Southern / Q000379:methods; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008654:Mesothelioma / Q000473:pathology* / Q000821:virology*; D008875:Middle Aged; D010232:Paraffin; D016133:Polymerase Chain Reaction / Q000379:methods; D012045:Regulatory Sequences, Nucleic Acid; D015203:Reproducibility of Results; D013539:Simian virus 40 / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral; D010232:Paraffin