Elimination of amino acid interferences in the chiral ligand-exchange chromatographic analysis of lactic acid enantiomers in wine.
Chiral ligand-exchange liquid chromatography is used to identify and quantitate lactic acid enantiomers in wines that have or have not undergone malolactic fermentation. The stationary phase is (R)-penicillamine, which is bound lipophilically to a C18 bonded silica matrix. The mobile phase is 1mM copper sulfate, and the detection mode is ultraviolet. Serious interference from (S)-aspartic acid and other amino acids is eliminated by the use of propanesulfonic acid-type cation exchange solid-phase extraction cartridges prior to chromatographic analysis. Lactic acid enantiomers in wine are quantitated in the range of 10 to 500 mg/L. The detection limit is 3 mg/L. The method is also successful in the determination of lactic acid enantiomers in certain beers (e.g., lambic beers), kim-chi, sauerkraut, and various yogurts.
Buglass
A J
AJ
School of Applied Sciences, Anglia Polytechnic University, Cambridge, UK.
Lee
S H
SH
D000596:Amino Acids / Q000032:analysis; D001224:Aspartic Acid / Q000032:analysis; D002412:Cations; D002853:Chromatography, Liquid / Q000379:methods*; D019327:Copper Sulfate; D005189:False Positive Reactions; D005285:Fermentation; D007202:Indicators and Reagents; D007474:Ion Exchange; D019344:Lactic Acid / Q000032:analysis*; D010396:Penicillamine; D011786:Quality Control; D012822:Silicon Dioxide; D013237:Stereoisomerism; D014920:Wine / Q000032:analysis*
D000596:Amino Acids; D002412:Cations; D007202:Indicators and Reagents; D001224:Aspartic Acid; D019344:Lactic Acid; D012822:Silicon Dioxide; D010396:Penicillamine; D019327:Copper Sulfate