title: The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p.
abstract: In Saccharomyces cerevisiae, mRNA encoding the cell-fate determinant Ash1p is localized to the distal tip of daughter cells. Five SHE genes are required for proper Ash1 mRNA localization, one of which encodes the myosin Myo4p. We show that three of the five She proteins, She2p, She3p, and Myo4p, colocalize with Ash1 mRNA in vivo and coimmunoprecipitate with Ash1 mRNA from cell extracts. We also find that She3p binds to Myo4p in the absence of RNA and She2p is required for binding She3p-Myo4p to Ash1 mRNA. These results suggest that She3p acts as an adapter protein that docks the myosin motor onto an Ash1-She2p ribonucleoprotein complex.
authors.affiliation: Department of Cellular and Molecular Pharmacology; and Howard Hughes Medical Institute, University of California, San Francisco, CA 94143-0450, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Takizawa
authors.forename: R D
authors.initials: RD
authors.lastname: Vale
mesh_terms: D001665:Binding Sites; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008954:Models, Biological; D018995:Myosin Heavy Chains; D024701:Myosin Type V; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism*; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification; D016335:Zinc Fingers
chemical_list: C098733:ASH1 protein, S cerevisiae; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C098732:Myo4 protein, S cerevisiae; D012331:RNA, Fungal; D012333:RNA, Messenger; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins