title: Microtubules regulate local Ca2+ spiking in secretory epithelial cells.
abstract: The role of the cytoskeleton in regulating Ca(2+) release has been explored in epithelial cells. Trains of local Ca(2+) spikes were elicited in pancreatic acinar cells by infusion of inositol trisphosphate through a whole cell patch pipette, and the Ca(2+)-dependent Cl(-) current spikes were recorded. The spikes were only transiently inhibited by cytochalasin B, an agent that acts on microfilaments. In contrast, nocodazole (5-100 micrometer), an agent that disrupts the microtubular network, dose-dependently reduced spike frequency and decreased spike amplitude leading to total blockade of the response. Consistent with an effect of microtubular disruption, colchicine also inhibited spiking but neither Me(2)SO nor beta-lumicolchicine, an inactive analogue of colchicine, had any effect. The microtubule-stabilizing agent, taxol, also inhibited spiking. The nocodazole effects were not due to complete loss of function of the Ca(2+) signaling apparatus, because supramaximal carbachol concentrations were still able to mobilize a Ca(2+) response. Finally, as visualized by 2-photon excitation microscopy of ER-Tracker, nocodazole promoted a loss of the endoplasmic reticulum in the secretory pole region. We conclude that microtubules specifically maintain localized Ca(2+) spikes at least in part because of the local positioning of the endoplasmic reticulum.
authors.affiliation: Department of Pharmacology, Cambridge University, Cambridge CB2 1QJ, United Kingdom.
authors.forename: K E
authors.initials: KE
authors.lastname: Fogarty
authors.forename: J F
authors.initials: JF
authors.lastname: Kidd
authors.forename: A
authors.initials: A
authors.lastname: Turner
authors.forename: J N
authors.initials: JN
authors.lastname: Skepper
authors.forename: J
authors.initials: J
authors.lastname: Carmichael
authors.forename: P
authors.initials: P
authors.lastname: Thorn
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000502:physiology* / Q000648:ultrastructure*; D051379:Mice; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure*; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D010179:Pancreas / Q000378:metabolism
chemical_list: D017239:Paclitaxel; D015739:Nocodazole; D003078:Colchicine; D002118:Calcium