In vitro identification of dividing neuronal precursors from chick embryonic ciliary ganglion.
In chick parasympathetic ciliary ganglion the neuronal birthdate is well defined, between 2.5 and 5.5 days of embryonic development, and neuronal precursor cells that are able to differentiate into neurons in vitro can be isolated from E4.5 ganglia. In this report, using bromodeoxyuridine incorporation and Maplb immunostaining, we demonstrate that these cells can be isolated from E7-E8 chick embryos as well, suggesting that neuronal precursor cells are still present in the ciliary ganglion after the end of the in vivo neurogenesis. These precursor cells retain the ability to divide and generate newly differentiated neurons in vitro when cultured in a chemically defined medium. Such a capacity is highly stimulated by bFGF but not by CNTF.
Gilardino
A
A
Dipartimento di Biologia Animale e dell'Uomo, Torino, Istituto Nazionale per la Fisica della Materia, Italy.
Perroteau
I
I
Lovisolo
D
D
Distasi
C
C
D000818:Animals; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics; D002452:Cell Count / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005726:Ganglia, Parasympathetic / Q000166:cytology* / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism / Q000494:pharmacology; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D020934:Ciliary Neurotrophic Factor; D007797:Laminin; D008869:Microtubule-Associated Proteins; C085284:microtubule-associated protein 1B; D016222:Fibroblast Growth Factor 2; D001973:Bromodeoxyuridine