Patterns of intraocular pressure elevation after aqueous humor outflow obstruction in rats.
OBJECTIVE
To determine the diural intraocular pressure (IOP) response of Brown Norway rat eyes after sclerosis of the aqueous humor outflow pathways and its relationship to optic nerve damage.


METHODS
Hypertonic saline was injected into a single episcleral vein in 17 animals and awake IOP measured in both the light and dark phases of the circadian cycle for 34 days. Mean IOP for light and dark phases during the experimental period were compared with the respective pressures of the uninjected fellow eyes. Optic nerve cross sections from each nerve were graded for injury by five independent masked observers.


RESULTS
For fellow eyes, mean light- and dark-phase IOP was 21 +/- 1 and 31 +/- 1 mm Hg, respectively. For four experimental eyes, mean IOPs for both phases were not altered. Six eyes demonstrated significant mean IOP elevations only during the dark phase. Of these, five showed persistent, large circadian oscillations, and four had partial optic nerve lesions. The remaining seven eyes experienced significant IOP elevations during both phases, and all had extensive optic nerve damage.


CONCLUSIONS
Episcleral vein injection of hypertonic saline is more likely to increase IOP during the dark phase than the light. This is consistent with aqueous outflow obstruction superimposed on a circadian rhythm of aqueous humor production. Because these periodic IOP elevations produced optic nerve lesions, both light- and dark-phase IOP determinations are necessary for accurate correlation of IOP history to optic nerve damage in animals housed in a light- dark environment.
Jia
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Kenneth C. Swan Ocular Neurobiology Laboratory, Casey Eye Institute, Oregon Health Sciences University, Portland, USA.
Cepurna
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Johnson
E C
EC
Morrison
J C
JC
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology; D007429:Intraocular Pressure / Q000502:physiology*; D008297:Male; D008954:Models, Biological; D009798:Ocular Hypertension / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D009900:Optic Nerve / Q000473:pathology; D009901:Optic Nerve Diseases / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011914:Rats, Inbred BN; D012462:Saline Solution, Hypertonic / Q000633:toxicity; D012590:Sclera / Q000098:blood supply; D012598:Sclerosis; D014129:Trabecular Meshwork / Q000473:pathology; D014680:Veins / Q000187:drug effects
D012462:Saline Solution, Hypertonic