Production of seafood flavor from red hake (Urophycis chuss) by enzymatic hydrolysis.
Protein hydrolysates were prepared as a natural flavor stock from the red hake (Urophycis chuss) headed-gutted (H&G) mince and frame mince using commercial enzymes, Flavourzyme and Savorase, at the natural pH of fish (6.8) and the water/fish ratio of 2:5. The addition of 1.5% NaCl and 0.4% STPP improved the flavor quality of the hydrolysate by masking bitterness and off-flavor. A 6 h hydrolysis of H&G mince with Flavourzyme yielded a hydrolysate of the highest acceptability. Hydrolysis increased the concentration of most free amino acids except Arg and His. Leu, Lys, and Arg were predominant free amino acids in the hydrolysates, whereas Leu and Arg were major ones in the cooking juice. The concentration of Glu responsible for umami taste was increased by 6-9 times upon hydrolysis. Hydrolysates contained higher percentages of free amino acids giving both umami and sweet tastes than did cooking juice.
Imm
J Y
JY
Department of Food Science and Nutrition, University of Rhode Island, Kingston 02881, USA.
Lee
C M
CM
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005399:Fishes; D005421:Flavoring Agents; D006801:Humans; D006867:Hydrolases; D006868:Hydrolysis; D017747:Seafood; D013649:Taste
D000596:Amino Acids; D005421:Flavoring Agents; D006867:Hydrolases