title: Purification and characterization of the hemagglutinin-neuraminidase of Porcine rubulavirus LPMV.
abstract: The Hemagglutinin-Neuraminidase (HN) from the LPMV strain of Porcine rubulavirus was purified from virions by ultracentrifugation in a continuous 20-60% sucrose gradient and by ion exchange chromatography. The HN is a glycoprotein of 66 kDa constituted by 50.5, 13.3 and 13.6% of non polar, uncharged polar, and charged polar amino acids, respectively. The HN contains 4% of carbohydrates, its glycannic portion is constituted by Man, Gal, GlcNAc, GalNAc, and Neu5Ac in 3:3:4:1:1 molar ratios. The HN possesses hemagglutinating activity in the presence of erythrocytes from several animal species, including human ABO, and treating the erythrocytes with neuraminidase or pronase abolishes this activity. The binding specificity of the purified HN was determined by hapten inhibition assays, indicating that the hemagglutinating activity of the HN is specific for sialic acid and Neu5Acalpha2,3Gal-containing structures.
authors.affiliation: Departamento de Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, México. ezenteno@servidor.unam.mx
authors.forename: J
authors.initials: J
authors.lastname: Reyes-Leyva
authors.forename: B
authors.initials: B
authors.lastname: Espinosa
authors.forename: G
authors.initials: G
authors.lastname: Santos
authors.forename: R
authors.initials: R
authors.lastname: Zenteno
authors.forename: J
authors.initials: J
authors.lastname: Hernández
authors.forename: V
authors.initials: V
authors.lastname: Vallejo
authors.forename: E
authors.initials: E
authors.lastname: Zenteno
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D015707:HN Protein / Q000737:chemistry* / Q000302:isolation & purification; D006386:Hemagglutination Tests; D006736:Horses; D006801:Humans; D051379:Mice; D011817:Rabbits; D051381:Rats; D019213:Rubulavirus / Q000737:chemistry*; D013552:Swine / Q000821:virology*
chemical_list: D002241:Carbohydrates; D015707:HN Protein