Fold-back tetraplex DNA species in DNase I-resistant DNA isolated from HeLa cells.
A DNase I-resistant DNA species has been isolated and purified from HeLa cells by gel electrophoresis. Our studies indicate that the DNase I-resistant DNA species was about 40-60 bp fragment sizes responding to double-strand DNA marker and has higher guanine content. The image of AFM showed that this species has been assumed to be tetraplex structure according to its apparent width and height. Its CD, UV spectrum also exhibited characteristics similar to some tetraplex structure, which was different from the standard duplex DNA. 32P-labeled probes (TTAGGG)4 and 5'-TGGGGAGGGTGGGGAGGGTGGGGAAGG-3' could be hybridized to purified DNase I-resistant species. All results suggest that the DNase I-resistant DNA species have at least two components, which adopt an intrastrand fold-back DNA tetraplex. Their sequences were similar to human telomere and human c-myc locus (NHE), respectively.
Cao
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Institute of Biophysics, Chinese Academy of Sciences, Beijing. caoeh@sun5.ibp.ac.cn
Sun
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Zhang
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Li
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Bai
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D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D054856:G-Quadruplexes; D016259:Genes, myc / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D018625:Microscopy, Atomic Force; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D016615:Telomere / Q000235:genetics; D014466:Ultraviolet Rays
D007477:Ions; D004247:DNA; D012964:Sodium; D003850:Deoxyribonuclease I; D011188:Potassium