title: Production of sexed lambs after biopsy of ovine blastocyts produced in vitro.
abstract: Embryos were generated by in vitro fertilization of in vitro-matured oocytes, cultured to the blastocyst stage, biopsied for sex determination by a PCR-based procedure, and transferred to synchronized recipients. Three out of 5 sheep (60%) were diagnosed pregnant, and 4 lambs of predicted sex were born.
authors.affiliation: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph.
authors.forename: H S
authors.initials: HS
authors.lastname: Kochhar
authors.forename: B C
authors.initials: BC
authors.lastname: Buckrell
authors.forename: J W
authors.initials: JW
authors.lastname: Pollard
authors.forename: W A
authors.initials: WA
authors.lastname: King
mesh_terms: D000818:Animals; D001755:Blastocyst; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011256:Pregnancy Outcome; D019849:Sex Determination Processes; D012756:Sheep / Q000502:physiology*