title: Application of an indirect ELISA to milk samples to identify cows with Mycoplasma bovis mastitis.
abstract: An indirect ELISA was used to detect antibodies to Mycoplasma bovis in milk samples collected from a herd with M bovis mastitis. Antibodies were detected in samples from nine cows which had developed clinical M bovis mastitis. Milk from only three consistently antigen-negative cows tested positive for M bovis antibodies. These results indicate the potential value of the indirect ELISA for the detection of cows which have recently developed M bovis mastitis during the early stages of an outbreak.
authors.affiliation: Central Veterinary Research Laboratory, Dublin, Ireland.
authors.forename: W J
authors.initials: WJ
authors.lastname: Byrne
authors.forename: H J
authors.initials: HJ
authors.lastname: Ball
authors.forename: N
authors.initials: N
authors.lastname: Brice
authors.forename: R
authors.initials: R
authors.lastname: McCormack
authors.forename: S E
authors.initials: SE
authors.lastname: Baker
authors.forename: R D
authors.initials: RD
authors.lastname: Ayling
authors.forename: R A
authors.initials: RA
authors.lastname: Nicholas
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D002417:Cattle; D004196:Disease Outbreaks / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000382:microbiology*; D009174:Mycoplasma / Q000276:immunology / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial