Nuclear accumulation of p53 protein and apoptosis induced by various anticancer agents, u.v.-irradiation and heat shock in primary normal human skin fibroblasts.
Relationship between nuclear accumulation of p53 protein and apoptosis induced by both DNA damaging agents (ultraviolet-irradiation and anticancer agents) and heat shock in normal human skin fibroblasts was investigated. Nuclear accumulation of p53 protein and apoptosis were induced dose-dependently by u.v. -irradiation and anticancer agents. Regarding heat shock treatment, intensive nuclear accumulation of p53 protein was induced by treatment at 43 degrees C for 45 min, but, apoptotic cells were never detected. These results indicated that accumulation of p53 protein and apoptosis induced by DNA damaging agents were considered to have a close relationship and the functions of induced p53 protein are different depending on the stimulating factor.
Chikayama
S
S
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto-city, Japan.
Sugano
T
T
Takahashi
Y
Y
Ikeda
M
M
Kimura
S
S
Kobayashi
Y
Y
Kondo
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D018869:Heat-Shock Response / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
D000970:Antineoplastic Agents; D016159:Tumor Suppressor Protein p53