title: Direct PCR of Cryptococcus neoformans MATalpha and MATa pheromones to determine mating type, ploidy, and variety: a tool for epidemiological and molecular pathogenesis studies.
abstract: Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than flow cytometry.
authors.affiliation: Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chaturvedi
authors.forename: B
authors.initials: B
authors.lastname: Rodeghier
authors.forename: J
authors.initials: J
authors.lastname: Fan
authors.forename: C M
authors.initials: CM
authors.lastname: McClelland
authors.forename: B L
authors.initials: BL
authors.lastname: Wickes
authors.forename: V
authors.initials: V
authors.lastname: Chaturvedi
mesh_terms: D003453:Cryptococcosis / Q000453:epidemiology*; D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics*; D006801:Humans; D017720:Molecular Epidemiology; D010675:Pheromones / Q000235:genetics*; D011003:Ploidies; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
chemical_list: D010675:Pheromones