Transport of nucleosides in Bacillus subtilis: characteristics of cytidine-uptake.
In Bacillus subtilis SMYW cytidine and uridine are transported by a common system. Transport of these substances requires metabolic energy. After 60 sec of (3)II-cytidine-uptake practically all accumulated radioactivity was found in phosphorylated products. Addition of ribonucleosides with inhibitory effect upon cytidine-uptake resulted in competitive type of inhibition while interference with deoxyribonucleosides was of hyperbolic competitive type. Adenosine possesses a high affinity to cytidine-transporting site but requires different system(s) for its own transport. Guanosine, adenine, cytosine and 5-nucleotides do not interfere with cytidine-uptake.
Kloudová
A
A
Fucik
V
V
D001412:Bacillus subtilis / Q000378:metabolism*; D001693:Biological Transport, Active; D003562:Cytidine / Q000378:metabolism*; D007700:Kinetics; D009705:Nucleosides / Q000378:metabolism
D009705:Nucleosides; D003562:Cytidine