title: Fatty acids enhanced tubermycin production by Pseudomonas strain 2HS.
abstract: A new microbial isolate, Pseudomonas 2HS, produced trace amounts of a greenish-yellow pigment when grown aerobically in a 1% yeast extract medium at 30 degrees C and shaken at 250 rpm for 5 days. In contrast, cells produced more greenish-yellow pigment (2.16 mg/15 ml culture) when grown in the presence of 0.5% 12-hydroxyoctadecanoic acid (w/v). The greenish-yellow pigment was identified as phenazine-1-carboxylic acid (tubermycin B), and the Pseudomonas 2HS was identified as P. aeruginosa 2HS. This is the first report that 12-hydroxyoctadecanoic, ricinoleic and other fatty acids can enhance the production of phenazine-1-carboxylic acid by a Pseudomonas species.
authors.affiliation: Department of Biological Science, Western Illinois University, Macomb 61455, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Keudell
authors.forename: J K
authors.initials: JK
authors.lastname: Huang
authors.forename: L
authors.initials: L
authors.lastname: Wen
authors.forename: W E
authors.initials: WE
authors.lastname: Klopfenstein
authors.forename: M O
authors.initials: MO
authors.lastname: Bagby
authors.forename: A C
authors.initials: AC
authors.lastname: Lanser
authors.forename: R D
authors.initials: RD
authors.lastname: Plattner
authors.forename: R E
authors.initials: RE
authors.lastname: Peterson
authors.forename: D
authors.initials: D
authors.lastname: Weisleder
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000096:biosynthesis*; D001711:Biotransformation; D008372:Manure / Q000382:microbiology; D010619:Phenazines / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012277:Ricinoleic Acids / Q000494:pharmacology; D012756:Sheep; D013229:Stearic Acids / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D008372:Manure; D010619:Phenazines; D012277:Ricinoleic Acids; D013229:Stearic Acids; C037165:1-phenazinecarboxylic acid; C030521:ricinoleic acid