Fatty acid membrane composition and activation of glycine-betaine transport in Lactococcus lactis subjected to osmotic stress.
Lactococcus lactis subsp. cremoris NCDO763 accumulates glycine-betaine (betaine) when submitted to an osmotic stress with NaCl. Betaine transport activity increases with the extent of the osmotic upshock but also with growth temperature, and supplementation of the medium by Tween-80. Fatty acid analysis of the lipid fraction of L. lactis NCDO763 reveals significant modifications of the fatty acid composition of the membrane when cells are submitted to osmotic stress, high temperature or Tween-80 medium supplementation. The main modification in L. lactis membrane fatty acid composition in response to high osmolality is the increase of Cyclopropane Fatty Acid (CFA) deltaC19:0, whereas Unsaturated/Saturated ratio remains unchanged.
Guillot
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Unité de Recherche de Biochimie et Structure des Protéines, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Obis
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Mistou
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D001622:Betaine / Q000378:metabolism*; D001692:Biological Transport; D005227:Fatty Acids / Q000032:analysis*; D013294:Lactococcus lactis / Q000737:chemistry / Q000378:metabolism*; D008563:Membrane Lipids / Q000032:analysis*; D011136:Polysorbates / Q000494:pharmacology; D013696:Temperature; D014882:Water-Electrolyte Balance
D005227:Fatty Acids; D008563:Membrane Lipids; D011136:Polysorbates; D001622:Betaine