title: Isolation of emetine resistant clones of Entamoeba histolytica by petri dish agar method.
abstract: Emetine resistant clones of Entamoeba histolytica strain HM1:IMSS were isolated by using petri dish agar method after mutation with ethyl-methanesulphonate. Two emetine resistant clones were obtained and both were resistant to emetine at a concentration of 24 micrograms/ml of emetine. The 50 per cent inhibitory concentration (IC50) for both emetine sensitive and resistant clones was 5 and 14 micrograms/ml respectively. The colony forming efficiency of E. histolytica strain HM1:IMSS varied from 44 to 54 per cent. This method is useful for isolating clones from different strains of the parasite for molecular and immunological studies.
authors.affiliation: Department of Parasitology, Postgraduate Institute of Medical Education & Research, Chandigarh.
authors.forename: R
authors.initials: R
authors.lastname: Prabhu
authors.forename: R
authors.initials: R
authors.lastname: Sehgal
authors.forename: A
authors.initials: A
authors.lastname: Chakraborti
authors.forename: N
authors.initials: N
authors.lastname: Malla
authors.forename: N K
authors.initials: NK
authors.lastname: Ganguly
authors.forename: R C
authors.initials: RC
authors.lastname: Mahajan
mesh_terms: D000362:Agar; D000563:Amebicides / Q000494:pharmacology*; D000818:Animals; D004352:Drug Resistance, Microbial / Q000235:genetics; D004640:Emetine / Q000494:pharmacology*; D004748:Entamoeba histolytica / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008828:Microbiological Techniques
chemical_list: D000563:Amebicides; D000362:Agar; D004640:Emetine