The cellular response of JC virus T-antigen-induced brain tumor implants to a Murine intra-ocular model.
In order to define the immunologic response to central nervous system tumors in a controlled fashion, we compared xenogeneic, allogeneic and syngeneic transplants of JC virus-induced neural tumor cell aggregates implanted into anterior ocular chambers of mice. Semiquantitative assessment of the level of leukocyte common antigen (CD45) of the transplants by immunohistochemistry was used to gauge rejection. Reticulin staining was used to monitor vascularization. Immunoreactivity to the viral oncoprotein, T-antigen, was confirmed by immunohistochemistry and immunoprecipitation/Western blot analysis. The results demonstrated that transplants were viable at all time-points and developed vascularization as early as three days after transplantation. Xenotransplants, 13-days post-transplantation, and allogeneic transplants, 25 days post-transplantation were infiltrated with polymorphonuclear leukocytes. Fewer CD45 positive cells were demonstrated in syngeneic transplants. High levels of JCV T-antigen stimulated rejection in syngeneic transplants. These results establish a model for further investigation of the natural and induced immunologic response to central nervous system tumors.
Croul
S
S
Center for NeuroVirology and NeuroOncology and Department of Neurology, MCP-Hahnemann University, 245 N 15th Street, Mail Stop 406, Philadelphia, PA 19102, USA.
Lublin
F D
FD
Del Valle
L
L
Oshinsky
R J
RJ
Giordano
A
A
Khalili
K
K
Ritchie
C K
CK
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D001254:Astrocytoma / Q000098:blood supply / Q000276:immunology / Q000473:pathology; D001808:Blood Vessels / Q000473:pathology; D001932:Brain Neoplasms / Q000098:blood supply / Q000276:immunology* / Q000473:pathology; D006224:Cricetinae; D006085:Graft Survival; D007577:JC Virus / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008527:Medulloblastoma / Q000098:blood supply / Q000276:immunology / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation / Q000276:immunology*; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic / Q000276:immunology*; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D014407:Tumor Cells, Cultured / Q000637:transplantation
D000957:Antigens, Viral, Tumor; D017493:Leukocyte Common Antigens