Agar gel immunodiffusion test for the detection of bovine leukemia virus antibodies: lack of trans-Atlantic standardization.
Two agar gel immunodiffusion (AGID) kits for the serodiagnosis of bovine leukemia virus (BLV) were imported from Europe and were compared with North American kits. The BLV AGID kits from North America and from Europe differed significantly. The punches were different, as were the pattern distribution in the agar of the reference and the test sera, resulting in differences in the reading of the immunoprecipitation lines. Based on the testing of 1200 serum samples from cattle, the European kits gave a good correlation with the American kits, as indicated by their respective kappa values. However, the European kits were found to be less sensitive when evaluated against weakly positive samples from field specimens or following a dilution trial. Only 65% and 50% of the weakly positive samples detected by the American kit #1 were detected by the European kits #2 and #3, respectively. The American kit was also capable of detecting BLV antibodies in 45% of strongly positive samples diluted 1/50 in negative sera, while antibodies were detected in only 15% of the samples with the European kit #2 and in none of the samples with the European kit #3. False negatives were also detected with the European kits. Among the false negatives, the degree of expected reactions was weak (European kit #2) or of varying degrees of positivity (European kit #3). Besides the differences in format and performance, the BLV-AGID kits in Europe are evaluated with the National Standard Serum E4 while a proficiency panel composed of a quadruplicate set of 10 reference sera is used in Canada to monitor the kits. Based on the overall observations, we noted a lack of standardization between the BLV-AGID kits used in North America and in Europe.
Simard
C
C
Retrovirology Centre of Expertise, Canadian Food Inspection Agency, Charlottetown, Prince Edward Island. simardc@em.agr.ca
Richardson
S
S
Dixon
P
P
Komal
J
J
D000362:Agar; D000818:Animals; D000906:Antibodies / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis*; D005260:Female; D005779:Immunodiffusion / Q000379:methods / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008297:Male; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
D000906:Antibodies; D000362:Agar