Alterations on polyamine content and glutathione metabolism induced by different concentrations of paraquat in CHO-K1 cells.
The effect of herbicide paraquat has been assessed on CHO-K1 cultures at different concentrations. Glutathione peroxidase, reductase and S-transferase, as well as total and oxidized glutathione, were evaluated along the standard growth curve of the cultures. Paraquat was then administered during mid-log phase at concentrations that produced a calculated lethality of 6.25%, 12.5% and 25%, using the lysosomal dye assay, neutral red. After 24hr of incubation with paraquat, glutathione peroxidase suffered a large dose-response increase, unlike glutathione reductase and S-transferase, the activities of which were lower than untreated controls. The profile of total glutathione content was similar to that found for glutathione peroxidase, increasing with the administered doses of the herbicide. Polyamine content has been also studied at the same concentrations of paraquat, showing that intracellular spermidine and spermine pools were negatively affected with paraquat in a dose-response manner, unlike putrescine, which maintained elevated pools at the three concentrations assayed.
Bayoumi
A E
AE
Dpt Plant Protection, Faculty of Agriculture, University Aim Shams, Cairo, Egypt.
Perez-Pertejo
Y
Y
Ordóñez
C
C
Reguera
R M
RM
Cubría
J C
JC
Balaña-Fouce
R
R
Ordóñez
D
D
D000818:Animals; D015317:Biogenic Polyamines / Q000032:analysis*; D016466:CHO Cells; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism*; D006540:Herbicides / Q000633:toxicity*; D010269:Paraquat / Q000633:toxicity*; D017382:Reactive Oxygen Species
D015317:Biogenic Polyamines; D006540:Herbicides; D017382:Reactive Oxygen Species; D005978:Glutathione; D010269:Paraquat