title: Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional diterpene cyclase.
abstract: We report here the nucleotide sequence of a full-length cDNA encoding ent-kaurene synthase that was isolated by a reverse-transcription polymerase chain reaction from Gibberella fujikuroi (Gcps/ks). This cDNA encodes 952 amino acid residues with a relative molecular mass of 107 kDa. The sequence similarity between Gcps/ks and ent-kaurene synthase of the gibberellin A1-producing fungus, Phaeosphaeria sp. L487, is very high, suggesting that Gcps/ks is also a bifunctional diterpene cyclase. Its recombinant protein expressed in Escherichia coli converted geranylgeranyl diphosphate to copalyl diphosphate and ent-kaurene.
authors.affiliation: Department of Bioresource Engineering, Yamagata University, Tsuruoka-shi, Japan. toyomasu@tds1.tr.yamagata-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Toyomasu
authors.forename: H
authors.initials: H
authors.lastname: Kawaide
authors.forename: A
authors.initials: A
authors.lastname: Ishizaki
authors.forename: S
authors.initials: S
authors.lastname: Shinoda
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
authors.forename: W
authors.initials: W
authors.lastname: Mitsuhashi
authors.forename: T
authors.initials: T
authors.lastname: Sassa
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005874:Gibberella / Q000201:enzymology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D019883:Alkyl and Aryl Transferases; C105712:ent-kaurene synthetase B