Analysis of novel metastasis-associated gene TI-227.
TI-227 is a cancer metastasis-associated gene isolated from the B16-F10 mouse melanoma subline that preferentially metastasizes to the lung following intravenous injection. TI-227 is more highly expressed in the B16-F10 and colon26-nl17 sublines, which preferentially metastasize to the lung following intravenous injection, than in the B16-BL6 and colon26-nl22 sublines, which preferentially metastasize to the lung following subcutaneous injection. Since TI-227 could not be detected in normal tissues, there is a possibility that it is expressed specifically in tumors that preferentially metastasize to the lung following intravenous inoculation. Although the 5' end of TI-227 was analyzed, its total sequence contained no obvious open reading frame. However comparison with the isolated human counterpart, TI-227H gene, revealed a highly homologous region near the 3' end, which was expected to contain a very short open reading frame.
Ishiguro
T
T
Department of Surgical Oncology, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
Nagawa
H
H
Naito
M
M
Tsuruo
T
T
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D008175:Lung Neoplasms / Q000556:secondary; D008546:Melanoma, Experimental / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics*; D016366:Open Reading Frames
D018076:DNA, Complementary