Identification of immunogenic antigens of Helicobacter pylori via the Escherichia coli hemolysin secretion system(1).
We describe a new procedure allowing the generation and detection of immunogenic antigens from Helicobacter pylori via the hemolysin secretion apparatus of Escherichia coli. The gene (or gene fragment) encoding the H. pylori protein (or protein domain) is inserted in-frame into a residual portion of the hemolysin gene (hlyA), encoding the HlyA secretion signal (HlyA(s)). These fusion proteins are secreted efficiently by E. coli. This new approach allows the identification of immunodominant antigens by using sera derived from H. pylori-infected patients suffering from different gastroduodenal pathologies. Three immunodominant antigens bearing the ureB (urease B-subunit), flaA (flagellin A-subunit), and an unknown ORF (HP0888) encoding an E. coli FecE analogous protein fused to hlyA(s) were identified and characterized.
Spreng
S
S
Theodor-Boveri-Institut, Lehrstuhl für Mikrobiologie, D-97074, Würzburg, Germany.
Gentschev
I
I
Goebel
W
W
Mollenkopf
H
H
Eck
M
M
Müller-Hermelink
H K
HK
Schmausser
B
B
D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005408:Flagellin / Q000235:genetics / Q000276:immunology; D005756:Gastritis / Q000097:blood / Q000276:immunology / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology*; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D018442:Lymphoma, B-Cell, Marginal Zone / Q000097:blood / Q000276:immunology / Q000382:microbiology; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000097:blood / Q000276:immunology / Q000382:microbiology; D014510:Urease / Q000235:genetics / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D011993:Recombinant Fusion Proteins; D005408:Flagellin; C067138:flaA protein, bacteria; D014510:Urease