[Fluorescence methods for studying cell damage].
We have been studying some properties of the new generation photosensitizers--phthalocyanines. The influence of the certain phthalocyanine concentrations at combination with laser irradiation doses (semiconductor laser, lambda = 670 nm, P = 50 mW) have been studied by in vitro fluorescent methods. Cytotoxicity assay LIVE/DEAD for fluorescence microscopy and measurements of cells viability with multi-well plate scanner was used. The optimal phototoxic effect on MCF7--mamma carcinoma cells was observed 8 micrograms/ml ZnPcS2, 10 micrograms/ml ClAlPcS2 in combination with laser irradiation 50 J/cm2 and 100 J/cm2 respectively. This concentration and dose killed MCF7 carcinoma cells.
Kolárová
H
H
Ustav lékarské biofyziky, Lékarské fakulty, Univerzita Palackého, Olomouc, Czech Republic.
Kotala
L
L
Strnad
M
M
Bancírová
M
M
Lasovský
J
J
Ambrozová
P
P
D001943:Breast Neoplasms / Q000473:pathology* / Q000532:radiotherapy; D002470:Cell Survival / Q000528:radiation effects; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D007834:Lasers; D008856:Microscopy, Fluorescence; D011829:Radiation Dosage; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007211:Indoles; D054833:Isoindoles; D011838:Radiation-Sensitizing Agents; C085925:calcein AM; C013647:phthalocyanine