title: Il-1, EGF, and HGF suppress the antiviral activity of interferon in primary monkey hepatic parenchymal cells.
abstract: The purpose of this study was to elucidate the mechanism behind low efficacy of interferon therapy to hepatitis C virus infected patients by using primary monkey hepatic parenchymal cells as a surrogate of primary human liver cells. The effects of various cytokines on the antiviral activity of IFNs in the monkey hepatic cells were studied to search for physiological inhibitors. Interleukin-1 alpha, EGF, and HGF showed suppressive effects on the antiviral activity of IFN-alpha, -beta in primary monkey hepatic cells when examined by the yield reduction method using vesicular stomatitis virus (VSV). In contrast, 50 ng/ml of TNF and IL-6 had no suppressive effect on the IFN-induced antiviral state in the hepatic cells.
authors.affiliation: Department of Viral Disease and Vaccine Control, National Institute of Infectious Diseases, Tokyo 208-0011, Japan. kohase@nih.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Takahara
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuyama
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Ogino
authors.forename: N
authors.initials: N
authors.lastname: Miyajima
authors.forename: M
authors.initials: M
authors.lastname: Kohase
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006133:Growth Substances / Q000494:pharmacology*; D000882:Haplorhini; D016174:Hepacivirus / Q000187:drug effects; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D016898:Interferon-alpha / Q000037:antagonists & inhibitors*; D016899:Interferon-beta / Q000037:antagonists & inhibitors*; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000821:virology*
chemical_list: D000998:Antiviral Agents; D006133:Growth Substances; D016898:Interferon-alpha; D007375:Interleukin-1; D015850:Interleukin-6; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D016899:Interferon-beta