International telemicroscopy with a 3 MV ultrahigh voltage electron microscope.
The ability for remote microscope operation via a network connection was added recently to the ultrahigh voltage electron microscope (UHVEM) in Osaka University, and used successfully for the observation of thick biological samples across the Pacific Ocean by researchers at the National Center for Microscopy and Imaging Research (NCMIR) at the University of California San Diego. High-quality images at video rate were transferred by a satellite link and control signals were transmitted by an ISDN connecting the workstations at both sites. Most microscope functions operated from the console of the UHVEM were replicated on the graphical user interface of the remote workstation. By clicking on icons or in boxes in the display window with a mouse, the researcher could operate the UHVEM from the remote-site. The total delay time for sending images and returning control signals was about 0.7 s, which did not interfere significantly with the smooth operation of the instrument. Researchers at the remote site were able to record images on film in the microscope which were later sent to San Diego.
Takaoka
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Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, Suita, Japan. takaoka@uhvem.osaka-u.ac.jp
Yoshida
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Mori
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Hayashi
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Young
S J
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Ellisman
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D000818:Animals; D001001:Anura; D002140:California; D005727:Ganglia, Spinal / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D007391:International Cooperation; D020407:Internet; D007564:Japan; D008854:Microscopy, Electron / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods; D009474:Neurons / Q000648:ultrastructure; D012371:Robotics; D017215:Satellite Communications