Use of novel bone biopsy system to study molecular effects of growth hormone in human bone--a pilot study.
In this study, we have examined whether a novel bone biopsy system combined with reverse transcription-polymerase chain reaction (RT-PCR) or differential display PCR (ddPCR) can be used to detect specific mRNAs induced by growth hormone (GH) in human bone. In a 58-year-old man with complete GH deficiency as a result of empty sella, bone biopsies were taken before, and 5 and 24 h after administration of 24 recombinant human GH. Insulin-like growth factor binding protein-3 (IGFBP-3) mRNA levels in this patient, measured in a semiquantitative RT-PCR assay, increased about 40% 24 h after GH administration. This increase was not seen in a healthy control who did not receive GH, suggesting that the increase was an effect of GH rather than of the biopsy itself. Several differentially expressed mRNAs were detected by ddPCR. Thus, this pilot study suggests that our novel bone biopsy system may be suitable for in vivo studies of the molecular effects of substances with essential functions in human bone.
Melhus
H
H
Department of Internal Medicine, University Hospital, Uppsala, Sweden. Hakan.Melhus@medicin.uu.se
Aström
G
G
Ahlström
H
H
Burman
P
P
Karlsson
A
A
Ljunghall
S
S
D001483:Base Sequence; D001706:Biopsy; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D004652:Empty Sella Syndrome / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D019382:Human Growth Hormone / Q000172:deficiency / Q000494:pharmacology*; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger; D019382:Human Growth Hormone