title: Lipid peroxidation and glutathione system in hyperlipemic rabbits: influence of olive oil administration.
abstract: We studied the effect of supplementation (10% w/w) of a hyperlipemic diet (1% cholesterol) with olive oil (OLIV) for 6 weeks in four groups of 10 rabbits each. At the end of this period, we determined lipid peroxidation, glutathione content, and glutathione peroxidase, reductase and transferase activities in liver, brain, heart, aorta and platelets. The atherogenic diet increased tissue lipid peroxidation and decreased the protective antioxidant effect of glutathione. Dietary supplementation with olive oil reduced tissue lipid peroxidation by 71.6% in liver, 20.3% in brain, 84.5% in heart, 63.6% in aorta, 72% in platelets. The ratios total/oxidized glutathione were increased in all tissues (49% in liver, 48% in brain, 45% in heart, 83% in aorta, 70% in platelets). Olive oil increased glutathione peroxidase and transferase activities in all tissues. We conclude that in rabbits made hyperlipemic with a diet rich in saturated fatty acids, olive oil decreased tissue oxidative stress.
authors.affiliation: Department of Pharmacology and Therapeutics, School of Medicine, University of Málaga, 29071, Málaga, Spain. jpcruz@uma.es
authors.forename: J P
authors.initials: JP
authors.lastname: De La Cruz
authors.forename: L
authors.initials: L
authors.lastname: Quintero
authors.forename: M A
authors.initials: MA
authors.lastname: Villalobos
authors.forename: F
authors.initials: F
authors.lastname: Sánchez de la Cuesta
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism; D001161:Arteriosclerosis / Q000517:prevention & control; D001792:Blood Platelets / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D004036:Diet, Atherogenic; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000032:analysis; D005978:Glutathione / Q000032:analysis / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006949:Hyperlipidemias / Q000097:blood / Q000378:metabolism* / Q000517:prevention & control; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D000069463:Olive Oil; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione