Detection of intracytoplasmic cytokines by flow cytometry.
Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models.
Santiago
M A
MA
Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil. santiago@gene.dbbm.fiocruz.br
Luca
P M
PM
Bertho
A L
AL
Azeredo-Coutinho
R B
RB
Coutinho
S G
SG
D000818:Animals; D016207:Cytokines / Q000032:analysis*; D003593:Cytoplasm / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007892:Leishmania braziliensis; D016773:Leishmaniasis, Cutaneous / Q000276:immunology
D016207:Cytokines