title: Performance of the ImmunoCard STAT! E. coli O157:H7 test for detection of Escherichia coli O157:H7 in stools.
abstract: ImmunoCard STAT! E. coli O157:H7 (Meridian Diagnostics, Inc., Cincinnati, Ohio) is a novel rapid (10-min) test for the presence of Escherichia coli O157:H7 in stools. The test may be performed either directly on stool specimens or on an overnight broth culture of stool. In a multicenter prospective study, 14 of 14 specimens positive by culture for E. coli O157:H7 were positive by the ImmunoCard STAT! O157:H7 test, and there were no false positives from 263 culture-negative specimens. In a retrospective study, the test was positive in 339 (81%) of 417 stored culture-positive specimens and the specificity was 95% (98 of 103 specimens). No false positives were associated with alternate stool pathogens. The ImmunoCard STAT! O157:H7 test has high sensitivity and specificity.
authors.affiliation: Child and Youth Clinical Trials Network, Children's Hospital of Eastern Ontario Research Institute, Ottawa, Canada. amackenzie@cyctn.on.ca
authors.forename: A
authors.initials: A
authors.lastname: Mackenzie
authors.forename: E
authors.initials: E
authors.lastname: Orrbine
authors.forename: L
authors.initials: L
authors.lastname: Hyde
authors.forename: M
authors.initials: M
authors.lastname: Benoit
authors.forename: F
authors.initials: F
authors.lastname: Chan
authors.forename: C
authors.initials: C
authors.lastname: Park
authors.forename: J
authors.initials: J
authors.lastname: Alverson
authors.forename: A
authors.initials: A
authors.lastname: Lembke
authors.forename: D
authors.initials: D
authors.lastname: Hoban
authors.forename: W
authors.initials: W
authors.lastname: Kennedy
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D011446:Prospective Studies; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
chemical_list: D011933:Reagent Kits, Diagnostic