Enhanced organically bound selenium yeast production by fed-batch fermentation.
This study describes a fed-batch fermentation protocol for enhanced production of Saccharomyces cerevisiae containing organically bound selenium. Two levels of sodium selenate concentrations were applied as either a single dose or continuous addition. Fermentations with high sodium selenate (63.2 g/L in cane molasses feeding medium) demonstrated 24 g/L of biomass with 1382 microg of selenium/g of dry biomass for single-dose addition and 40 g/L of biomass with 1491 microg of selenium/g of dry biomass for continuous addition. Low selenium concentration (31.6 g/L in cane molasses feeding medium) demonstrated higher biomass concentration with higher selenium level; 37 g/L of biomass with 2846 microg of selenium/g of dry biomass and 45 g/L of biomass with 2495 microg of selenium/g of dry biomass for single-dose and continuous addition, respectively. Also, two adapted S. cerevisiae strains were evaluated in fed-batch fermentation. A single dose of low concentration demonstrated >3000 microg of selenium/g of dry biomass, but biomass concentration was lower (< or =32 g/L) for these adapted strains.
Demirci
A
A
Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA.
Pometto
A L
AL
Cox
D J
DJ
D001152:Arsenicals / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D005524:Food Technology / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014131:Trace Elements
D001152:Arsenicals; D003470:Culture Media; D014131:Trace Elements