Serum amyloid A-derived peptides, present in human rheumatic synovial fluids, induce the secretion of interferon-gamma by human CD(4)(+) T-lymphocytes.
Serum amyloid A (SAA) is a major acute-phase protein whose biochemical functions remain largely obscure. Human rheumatic synovial fluids were screened by high performance liquid chromatography mass spectrometry for SAA-derived peptides, specifically the sequence AGLPEKY (SAA(98-104)) which was previously shown to modulate various leukocyte functions. Two such fluids were found to contain a truncated version of SAA(98-104). Synthetic SAA(98-104) and several of its analogs were shown capable of binding isolated human CD(4)(+) T-lymphocytes and stimulating them to produce interferon-gamma. Given the high acute-phase serum level of SAA and its massive proteolysis by inflammatory related enzymes, SAA-derived peptides may be involved in host defense mechanisms.
Yavin
E J
EJ
Department of Organic Chemistry, The Weizmann Institute of Science, Rehovot, Israel.
Preciado-Patt
L
L
Rosen
O
O
Yaron
M
M
Suessmuth
R D
RD
Levartowsky
D
D
Jung
G
G
Lider
O
O
Fridkin
M
M
D001053:Apolipoproteins / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D000685:Serum Amyloid A Protein / Q000276:immunology*; D013582:Synovial Fluid / Q000276:immunology
D001053:Apolipoproteins; D010455:Peptides; D000685:Serum Amyloid A Protein; D007371:Interferon-gamma