title: Effect of hyperthermia on the viability and the fibrinolytic potential of human cancer cell lines.
abstract: The effects of heat treatment on the viability and fibrinolytic potential of four cultured human carcinoma cell lines, fibrosarcoma cells (HT-1080), lung adenocarcinoma cells with highly metastatic potential (HAL-8), melanoma cells (Bowes) and osteosarcoma cells (NY), determined by measuring their levels of urokinase-type plasminogen activator (u-PA) and its specific receptor (u-PAR), were investigated by comparing them with those of human umbilical vein endothelial cells (HUVECs). HUVECs incubated at 43 degrees C for 120 min exhibited no decrease in viability but exhibited an increase in both u-PA and u-PAR. HT-1080 and HAL-8 showed a moderately high heat-resistance (viability, 60-90%) that correlated with the reduction of u-PAR but not u-PA. On the other hand, Bowes and NY cells, with poor heat-resistance (viability, 20-50%), exhibited stronger cell-associated u-PA activity when they survived at 43 degrees C for 120 min. Since the u-PA/u-PAR system is directly involved in the invasiveness and metastatic potential of carcinoma cells, hyperthermia would alter the biological activity of these carcinoma cells.
authors.affiliation: Department of Physiology, Kinki University School of Medicine, 377-2 Ohnohigashi, Osakasayama City, Osaka, Japan. fukao@med.kindai.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Fukao
authors.forename: M
authors.initials: M
authors.lastname: Ikeda
authors.forename: T
authors.initials: T
authors.lastname: Ichikawa
authors.forename: H
authors.initials: H
authors.lastname: Inufusa
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: S
authors.initials: S
authors.lastname: Ueshima
authors.forename: O
authors.initials: O
authors.lastname: Matsuo
mesh_terms: D000230:Adenocarcinoma / Q000503:physiopathology; D015152:Blotting, Northern; D002470:Cell Survival; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000378:metabolism; D005342:Fibrinolysis; D005354:Fibrosarcoma / Q000503:physiopathology; D006358:Hot Temperature; D006801:Humans; D008175:Lung Neoplasms / Q000503:physiopathology; D008545:Melanoma / Q000503:physiopathology; D009369:Neoplasms / Q000503:physiopathology*; D012516:Osteosarcoma / Q000503:physiopathology; D010960:Plasminogen Activators / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; C524898:PLAUR protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator