title: Flowcell culture of Porphyromonas gingivalis biofilms under anaerobic conditions.
abstract: We have developed an anaerobic biofilm culture system. The system is inexpensive, simple to use and, unlike an anaerobic glovebox, requires no dedicated space. As a test of the system, Porphyromonas gingivalis was cultured under low oxygen (1-2 ppm) and under anaerobic conditions (</=0.1 ppm O(2)). In the presence of small amounts of oxygen, the organism attached and formed an initial biofilm over the course of 4 h, but the biofilm was unable to maintain its growth and had lost biomass after 18 h. Also, ambiguous results were obtained when the biofilm was stained with a viability stain. Under anaerobic conditions, the biofilm was able to continue growth - biomass was greater after 18 h than after 4 h, and the anaerobic biofilm had a less ambiguous staining pattern than did the low-O(2)-grown biofilm.
authors.affiliation: Department of Microbiology, Technical University of Denmark, DK-2800, Lyngby, Denmark.
authors.forename: M C
authors.initials: MC
authors.lastname: Hansen
authors.forename: R J
authors.initials: RJ
authors.lastname: Palmer
authors.forename: D C
authors.initials: DC
authors.lastname: White
mesh_terms: D000693:Anaerobiosis; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D018441:Biofilms / Q000254:growth & development*; D018533:Biomass; D003470:Culture Media; D016966:Porphyromonas gingivalis / Q000502:physiology*
chemical_list: D003470:Culture Media