title: Genomic differentiation of Neanderthals and anatomically modern man allows a fossil-DNA-based classification of morphologically indistinguishable hominid bones.
abstract: Southern blot hybridizations of genomic DNA were introduced as a relatively simple fossil-DNA-based approach to classify remains of Neanderthals. When hybridized with genomic DNA of either human or Neanderthal origin, DNA extracted from two Neanderthal finds-the Os parietale, from Warendorf-Neuwarendorf, Germany, and a clavicula, from Krapina, Croatia-was shown to yield hybridization signals that differ by at least a factor of two compared to the signals obtained with the use of fossil DNA of an early Homo sapiens from the Vogelherd cave (Stetten I), Germany. When labeled chimpanzee DNA was used as a probe, Neanderthal and human DNA, however, revealed hybridization signals of similar intensity. Thus, the genome of Neanderthals is expected to differ significantly from the genome of anatomically modern man, because of the contrasting composition of repetitive DNA. These data support the hypothesis that Neanderthals were not ancestors of anatomically modern man.
authors.affiliation: Osteological Collection, University of Tübingen, Tübingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Scholz
authors.forename: L
authors.initials: L
authors.lastname: Bachmann
authors.forename: G J
authors.initials: GJ
authors.lastname: Nicholson
authors.forename: J
authors.initials: J
authors.lastname: Bachmann
authors.forename: I
authors.initials: I
authors.lastname: Giddings
authors.forename: B
authors.initials: B
authors.lastname: Rüschoff-Thale
authors.forename: A
authors.initials: A
authors.lastname: Czarnetzki
authors.forename: C M
authors.initials: CM
authors.lastname: Pusch
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D001842:Bone and Bones / Q000737:chemistry*; D002968:Clavicle / Q000737:chemistry; D017523:Croatia; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification; D005269:Femur / Q000737:chemistry; D005580:Fossils; D005858:Germany; D049690:History, Ancient; D015186:Hominidae / Q000145:classification* / Q000235:genetics*; D006801:Humans; D006811:Humerus / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D002679:Pan troglodytes / Q000235:genetics; D012053:Reindeer / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D013237:Stereoisomerism
chemical_list: D015342:DNA Probes; D004272:DNA, Mitochondrial; D004247:DNA