title: Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli.
abstract: An alternative mevalonate-independent pathway for isoprenoid biosynthesis has been recently discovered in eubacteria (including Escherichia coli) and plant plastids, although it is not fully elucidated yet. In this work, E. coli cells were engineered to utilize exogenously provided mevalonate and used to demonstrate by a genetic approach that branching of the endogenous pathway results in separate synthesis of the isoprenoid building units isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In addition, the IPP isomerase encoded by the idi gene was shown to be functional in vivo and to represent the only possibility for interconverting IPP and DMAPP in this bacterium.
authors.affiliation: Department de Bioquimica i Biologia Molecular, Facultat de Quimica, Universitat de Barcelona, Marti i Franquès 1-7, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Concepción
authors.forename: N
authors.initials: N
authors.lastname: Campos
authors.forename: L
authors.initials: L
authors.lastname: María Lois
authors.forename: C
authors.initials: C
authors.lastname: Maldonado
authors.forename: J F
authors.initials: JF
authors.lastname: Hoeffler
authors.forename: C
authors.initials: C
authors.lastname: Grosdemange-Billiard
authors.forename: M
authors.initials: M
authors.lastname: Rohmer
authors.forename: A
authors.initials: A
authors.lastname: Boronat
mesh_terms: D001089:Arabinose / Q000378:metabolism; D019748:Carbon-Carbon Double Bond Isomerases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D045782:Hemiterpenes; D008798:Mevalonic Acid / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009943:Organophosphorus Compounds / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D045782:Hemiterpenes; D009943:Organophosphorus Compounds; C004809:isopentenyl pyrophosphate; C043060:3,3-dimethylallyl pyrophosphate; D001089:Arabinose; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase; D008798:Mevalonic Acid