Cloning and characterization of amphibian cold inducible RNA-binding protein.
Gene expression of cold inducible RNA-binding protein (CIRP) was examined in the frog. In Xenopus laevis, expression of CIRP (XCIRP) was observed in both brain and liver at 24 degrees C. Circadian expression of XCIRP was observed in brain. Expression of XCIRP in brain was induced by cold treatment and gradually decreased to the control level at 24 degrees C, but no significant changes were observed in liver. Employing the sequence of murine CIRP, bullfrog (Rana catesbeiana) CIRP gene was cloned. The bullfrog CIRP gene, designated BFCIRP, was 706 bp in length and encoded a putative protein of 164 amino acid residues. The deduced protein contained one consensus sequence of RNA-binding domain (CS-RBD) and a glycine rich domain (GRD). The amino acid sequence of BFCIRP was 78.4% identical to XCIRP. Expression of BFCIRP in brain was stronger in winter than that in summer. These findings suggest that BFCIRP expression in brain may link to hibernation.
Saito
T
T
Department of Cell Science, Faculty of Graduated School of Science and Technology, Niigata University, Japan.
Sugimoto
K
K
Adachi
Y
Y
Wu
Q
Q
Mori
K J
KJ
D000595:Amino Acid Sequence; D029845:Amphibian Proteins; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006605:Hibernation; D006801:Humans; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011892:Rana catesbeiana / Q000235:genetics*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D016415:Sequence Alignment; D013696:Temperature; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000235:genetics* / Q000378:metabolism
D029845:Amphibian Proteins; C112949:CIRBP protein, Xenopus; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins; C415123:cold inducible RNA-binding protein, Rana catesbeiana