Rapid detection of malto-oligosaccharide-forming bacterial amylases by high performance anion-exchange chromatography.
High performance anion-exchange chromatography with pulsed amperometric detection was applied for the rapid analysis of malto-oligosaccharides formed by extracellular enzyme preparations from 49 starch-degrading bacterial strains isolated from soil and compost samples. Malto-oligosaccharide-forming amylases, indicated by a predominant formation of maltohexaose from starch, were produced by enzyme preparations from four of the isolates growing at pH 7.0 and 10.
Duedahl-Olesen
L
L
Biotechnology Laboratory, Department of Civil Engineering, Aalborg University, Aalborg, Denmark.
Larsen
K L
KL
Zimmermann
W
W
D000681:Amylases / Q000302:isolation & purification* / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D008320:Maltose / Q000031:analogs & derivatives / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism*; D012988:Soil Microbiology; D013213:Starch / Q000378:metabolism
D009844:Oligosaccharides; D008320:Maltose; D013213:Starch; D000681:Amylases