title: Development of new biotin/streptavidin reagents for pretargeting.
abstract: The high affinity of biotin for streptavidin has made this pair of molecules very useful for in vivo applications. To optimize reagents for one potential in vivo application, antibody-based pretargeting of cancer, we have prepared a number of new biotin and streptavidin derivatives. The derivatives developed include new radiolabeled biotin reagents, new protein biotinylation reagents, and new biotin multimers for cross-linking and/or polymerization of streptavidin. We have also modified streptavidin by site-directed mutation and chemical modification to improve its in vivo characteristics, and have developed new reagents for cross-linking antibody fragments with streptavidin. A brief overview of these new reagents is provided.
authors.affiliation: Department of Radiation Oncology, University of Washington, Seattle 98195, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Wilbur
authors.forename: P M
authors.initials: PM
authors.lastname: Pathare
authors.forename: D K
authors.initials: DK
authors.lastname: Hamlin
authors.forename: P S
authors.initials: PS
authors.lastname: Stayton
authors.forename: R
authors.initials: R
authors.lastname: To
authors.forename: L A
authors.initials: LA
authors.lastname: Klumb
authors.forename: K R
authors.initials: KR
authors.lastname: Buhler
authors.forename: R L
authors.initials: RL
authors.lastname: Vessella
mesh_terms: D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D001710:Biotin / Q000737:chemistry; D003432:Cross-Linking Reagents; D006801:Humans; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D016297:Mutagenesis, Site-Directed; D009369:Neoplasms / Q000532:radiotherapy; D015202:Protein Engineering; D020836:Protein Structure, Quaternary; D016499:Radioimmunotherapy; D019809:Streptavidin / Q000737:chemistry / Q000235:genetics
chemical_list: D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D001710:Biotin; D019809:Streptavidin