title: Oxidant stress in sled dogs subjected to repetitive endurance exercise.
abstract: OBJECTIVE
  To determine whether repetitive endurance exercise in sled dogs was associated with substantial lipid peroxidation, decreases in antioxidant capacity of the serum, and skeletal muscle damage.
  
  
  METHODS
  24 lightly trained sled dogs.
  
  
  METHODS
  16 dogs completed a 58-km run on each of 3 consecutive days; the other 8 dogs (control) did not exercise during the study. Blood samples were collected before the first exercise run and after the first and third exercise runs. Plasma isoprostane and serum vitamin E concentrations, total antioxidant status of plasma, and serum creatine kinase activity were measured.
  
  
  RESULTS
  Plasma isoprostane concentrations in dogs in the exercise group were significantly increased after the first exercise run and further significantly increased after the third exercise run. Serum vitamin E concentration was significantly decreased after the first exercise run in dogs in the exercise group, and this change persisted after the third exercise run. There was a significant linear relationship between plasma isoprostane concentration and the logarithm of serum creatine kinase activity (adjusted ? = 0.84).
  
  
  CONCLUSIONS
  Results demonstrate that repetitive endurance exercise in dogs is associated with lipid peroxidation and a reduction in plasma antioxidant concentrations. We interpret these results as indicating that the antioxidant mechanisms of minimally trained dogs may, in some instances, be inadequate to meet the antioxidant requirements of repetitive endurance exercise.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus 43210, USA.
authors.forename: K W
authors.initials: KW
authors.lastname: Hinchcliff
authors.forename: G A
authors.initials: GA
authors.lastname: Reinhart
authors.forename: R
authors.initials: R
authors.lastname: DiSilvestro
authors.forename: A
authors.initials: A
authors.lastname: Reynolds
authors.forename: A
authors.initials: A
authors.lastname: Blostein-Fujii
authors.forename: R A
authors.initials: RA
authors.lastname: Swenson
mesh_terms: D000821:Animal Feed; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002570:Ceruloplasmin / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D004285:Dogs / Q000502:physiology*; D028441:F2-Isoprostanes; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011897:Random Allocation; D012044:Regression Analysis; D012420:Running / Q000502:physiology; D013482:Superoxide Dismutase / Q000097:blood; D014527:Uric Acid / Q000097:blood; D014810:Vitamin E / Q000097:blood
chemical_list: D001798:Blood Proteins; D028441:F2-Isoprostanes; D014810:Vitamin E; D014527:Uric Acid; C075750:8-epi-prostaglandin F2alpha; D002784:Cholesterol; D015237:Dinoprost; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin; D003402:Creatine Kinase