IGF-I expression is decreased in LIF-deficient mice after peripheral nerve injury.
We investigated the regulation of insulin-like growth factor 1 (IGF-1) expression after sciatic nerve crush using leukemia inhibitory factor (LIF)-deficient mice. One day post-crush, IGF-1 mRNA levels were lower in the LIF-deficient mouse nerve than in the wild type nerve. IGF-1 protein, analyzed by immunohistochemistry, was also decreased 1 day post-crush in LIF-deficient nerves relative to wild type nerves. By 3 days post-crush, IGF-1 immunoreactivity was induced in Schwann cells to equivalent levels in both types of nerve. After crush, IGF-1 expression was also found in mast cells, and these were initially decreased in the LIF-deficient mice. Thus, LIF appears to regulate IGF-1 expression in the peripheral nerve basally and early in the regeneration response in vivo.
de Pablo
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F
Centro de Investigaciones Biològicas, CSIC, Madrid, Spain.
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Patterson
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D000818:Animals; D001369:Axons / Q000473:pathology; D002452:Cell Count; D002470:Cell Survival / Q000235:genetics; D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006131:Growth Inhibitors / Q000172:deficiency* / Q000235:genetics; D007150:Immunohistochemistry; D007211:Indoles; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000172:deficiency* / Q000235:genetics; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000502:physiology; D010147:Pain Measurement; D059348:Peripheral Nerve Injuries; D010525:Peripheral Nerves / Q000378:metabolism* / Q000601:surgery; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000378:metabolism; D012584:Sciatic Nerve / Q000502:physiology / Q000601:surgery; D012701:Serotonin / Q000378:metabolism; D014949:Wounds, Nonpenetrating / Q000378:metabolism*
D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D006131:Growth Inhibitors; D007211:Indoles; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D012333:RNA, Messenger; D012701:Serotonin; C007293:DAPI; D007334:Insulin-Like Growth Factor I