The protein expression of Bcl-2, Bax, Fas/Apo-1 in acute myeloid leukemia.
The protein expression of bcl-2, bax, Fas/Apo-1 in 19 cases of acute myeloid leukemia (AML) were investigated by Western blot and ApAAp techniques. High expression of bcl-2 protein was found in most of the AML cases, and some of the cases expressed Fas/Apo-1 and bax. High expression of bcl-2 protein was associated with a bad clinical prognosis and a poor response after intensive chemotherapy. Bax seemed to coexpress with bcl-2 and so appeared to be a bad prognostic factor instead of a good one. The expression of Fas/Apo-1 was inversely correlated with bcl-2 and seemed to be a good prognostic factor which may reflect the relative homeostasis of apoptotic pathway. It is concluded that apoptosis-induced pathways in AML often exhibit disturbance features. Coregulation of bcl-2, Bax and Fas/Apo-1 genes formed the apoptosis-induced pathway, which is the biological factor affecting response to chemotherapy.
Zhou
J
J
Department of Hematology, Tongji Hospital, Tongji Medical University, Wuhan.
Chen
Y
Y
Li
C
C
Liu
W
W
D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000096:biosynthesis; D005260:Female; D019254:Genes, bcl-2; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D051028:bcl-2-Associated X Protein
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C494826:BAX protein, human; D002352:Carrier Proteins; C490780:FAF1 protein, human; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein