Evaluation of chemiluminescent estradiol conjugates by using a surface plasmon resonance detector.
A series of chemiluminescent 17beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K(D)) for the interaction of an anti-E(2) Fab fragment for the probes in solution were evaluated using a single E(2)-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The results show the antibody fragment binds all chemiluminescent conjugates tested with high affinity showing only minor preferences for site of substitution (C6 versus C7), stereochemistry (alpha versus beta), or linker moiety.
Adamczyk
M
M
Department of Chemistry, Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, Illinois, USA. maciej.adamczyk@abbott.com
Chen
Y Y
YY
Gebler
J C
JC
Johnson
D D
DD
Mattingly
P G
PG
Moore
J A
JA
Reddy
R E
RE
Wu
J
J
Yu
Z
Z
D000911:Antibodies, Monoclonal / Q000378:metabolism; D015374:Biosensing Techniques; D004958:Estradiol / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D008163:Luminescent Measurements; D020349:Surface Plasmon Resonance / Q000379:methods*
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D004958:Estradiol