Investigation of electroplated conducting polymers as antibody receptors in immunosensors.
Antibodies raised against the conducting polymer, carbazole as a hapten, react to modulate the polymer's electrochemistry. Using cyclic voltammetry the reaction of the antiserum was discovered to influence the polymer matrix's electrochemistry by an amperometric response. It is suggested that these observation form the basis of a direct sensor for immunoassay.
Porter
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RA
Unilever Research Colworth, Colworth House Sharnbook, Bedford. Robert.Porter@unilever.com
D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002227:Carbazoles / Q000737:chemistry* / Q000276:immunology; D003505:Cyclodextrins / Q000737:chemistry; D004563:Electrochemistry; D004595:Electroplating / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D007124:Immunoenzyme Techniques; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding / Q000276:immunology; D011817:Rabbits; D015203:Reproducibility of Results; D047392:beta-Cyclodextrins
D000906:Antibodies; D002227:Carbazoles; D003505:Cyclodextrins; D006241:Haptens; D010940:Plant Proteins; D011108:Polymers; D047392:beta-Cyclodextrins; C041514:carbazole; D000469:Alkaline Phosphatase; C031215:betadex