A new system for three-dimensional tracking of motile microorganisms.
A new three-dimensional (3D)-tracking system with optimized dark-field illumination is presented. It allows simultaneous 3D tracking of several free-swimming microorganisms with diameters of >10 microm. Resolution limits and illumination efficiencies for different size classes of microorganisms are treated analytically. First applications for 3D tracking of protists are demonstrated.
Thar
R
R
Marine Biological Laboratory, University of Copenhagen, 3000 Helsingor, Denmark. roland.thar@gmx.net
Blackburn
N
N
Kühl
M
M
D000818:Animals; D004141:Dinoflagellida / Q000502:physiology*; D004867:Equipment Design; D005056:Euglena gracilis / Q000502:physiology*; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods*; D009068:Movement; D015203:Reproducibility of Results