Prediction of early renal graft function by the measurement of donor urinary glutathione S-transferases.
BACKGROUND
We have investigated the possibility of urinary alpha- and pi class glutathione S-transferases (GST-a; GST-pi) serving as a valuable parameter to predict early graft function after transplantation.


METHODS
Urinary GST concentrations of 61 donors (DON) and recipients (REC) were analyzed at preoperative, intraoperative, and postoperative periods. We grouped recipients according to the early postoperative graft recovery days.


RESULTS
The donor graft function, represented by the donor urinary GST concentration (GST-pi:17,1+/-12 microg/l mmol creatinine (crea); GST-a:14,3+/-10 microg/mmol crea), sustained a loss in comparison to the healthy controls (GST-a; pi< or =1 microg/mmol crea). According to statistical analysis, the donor GST-pi level showed a strong correlation with graft recovery days-pi (r = 0.84; P<0.001). The early graft function cannot be predicted by means of cold ischemia time (22.8+/-3.4 hr), nor handling time (42.4+/-11.1 min), nor even the intraoperative enzyme concentrations. The GST-pi cut off level (12.55 microg/mmol crea) might predict the possible posttransplant graft dysfunction. The discriminative analysis showed that using only DON GST-pi alone could discriminate well between the groups among all grafts in 68%.


CONCLUSIONS
Prognosis is poorer if the donor GST-pi concentration is above 12.55 microg/mmol crea. On the basis of the determination of GST-pi concentration in the donor urine, we can predict graft viability before the surgical procedure with a reliability of 68%.
Sárváry
E
E
Department of Transplantation and Surgery, Semmelweis University of Medicine, Budapest, Hungary. sarvary@mailtrans.sote.hu
Nemes
B
B
Járay
J
J
Dinya
E
E
Borka
P
P
Varga
M
M
Sulyok
B
B
Remport
A
A
Tóth
A
A
Perner
F
F
D000328:Adult; D002102:Cadaver; D016002:Discriminant Analysis; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000652:urine*; D006085:Graft Survival; D006801:Humans; D007527:Isoenzymes / Q000652:urine*; D007668:Kidney / Q000503:physiopathology*; D016030:Kidney Transplantation; D008875:Middle Aged; D009994:Osmolar Concentration; D011379:Prognosis; D013997:Time Factors; D014019:Tissue Donors
D007527:Isoenzymes; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C119995:glutathione S-transferase alpha