title: Evaluation of the role for prolactin-releasing peptide in prolactin secretion induced by ether stress and suckling in the rat: comparison with vasoactive intestinal peptide.
abstract: Prolactin (PRL)-releasing peptide (PrRP) is a recently discovered hypothalamic peptide possessing a specific stimulatory action on PRL secretion. In this study, we examined whether PrRP plays a role in mediating ether stress- and suckling-induced PRL secretion in rats through administering anti-PrRP antisera intracerebroventricularly. For comparison, we also tested the effect of anti-vasoactive intestinal peptide (VIP) antisera on the hormonal responses, since VIP is another candidate for a physiological PRL-releasing factor. The immunoneutralization of VIP, but not of PrRP, led to a significant suppression of PRL responses to both ether and suckling. These results suggest that PrRP may not play a significant role, or at least play a much weaker role than VIP, in mediating PRL release induced by ether stress and suckling in the rat.
authors.affiliation: Third Department of Internal. Medicine, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori, Japan. watanobe@infoaomori.ne.jp
authors.forename: H
authors.initials: H
authors.lastname: Watanobe
authors.forename: H B
authors.initials: HB
authors.lastname: Schiöth
authors.forename: J E
authors.initials: JE
authors.lastname: Wikberg
authors.forename: T
authors.initials: T
authors.lastname: Suda
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000494:pharmacology; D000918:Antibody Specificity; D005260:Female; D007028:Hypothalamic Hormones / Q000276:immunology / Q000378:metabolism*; D007276:Injections, Intraventricular; D008297:Male; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D009500:Neutralization Tests; D011247:Pregnancy; D011388:Prolactin / Q000097:blood / Q000378:metabolism*; D056690:Prolactin-Releasing Hormone; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000503:physiopathology*; D013391:Sucking Behavior / Q000502:physiology*; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000276:immunology / Q000378:metabolism*
chemical_list: D000906:Antibodies; D007028:Hypothalamic Hormones; D009479:Neuropeptides; C534379:Prlh protein, rat; D056690:Prolactin-Releasing Hormone; D014660:Vasoactive Intestinal Peptide; D011388:Prolactin