title: Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex.
abstract: Nup116p is a GLFG nucleoporin involved in RNA export processes. We show here that Nup116p physically interacts with the Nup82p-Nsp1p-Nup159p nuclear pore subcomplex, which plays a central role in nuclear mRNA export. For this association, a sequence within the C-terminal domain of Nup116p that includes the conserved nucleoporin RNA-binding motif was sufficient and necessary. Consistent with this biochemical interaction, protein A-Nup116p and the protein A-tagged Nup116p C-terminal domain, like the members of the Nup82p complex, localized to the cytoplasmic side of the nuclear pore complex, as revealed by immunogold labeling. Finally, synthetic lethal interactions were found between mutant alleles of NUP116 and all members of the Nup82p complex. Thus, Nup116p consists of three independent functional domains: 1) the C-terminal part interacts with the Nup82p complex; 2) the Gle2p-binding sequence interacts with Gle2p/Rae1p; and 3) the GLFG domain interacts with shuttling transport receptors such as karyopherin-beta family members.
authors.affiliation: Biochemie-Zentrum Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
authors.forename: S M
authors.initials: SM
authors.lastname: Bailer
authors.forename: C
authors.initials: C
authors.lastname: Balduf
authors.forename: J
authors.initials: J
authors.lastname: Katahira
authors.forename: A
authors.initials: A
authors.lastname: Podtelejnikov
authors.forename: C
authors.initials: C
authors.lastname: Rollenhagen
authors.forename: M
authors.initials: M
authors.lastname: Mann
authors.forename: N
authors.initials: N
authors.lastname: Pante
authors.forename: E
authors.initials: E
authors.lastname: Hurt
mesh_terms: D002135:Calcium-Binding Proteins; D016764:Cell Polarity; D003593:Cytoplasm; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013205:Staphylococcal Protein A / Q000235:genetics / Q000378:metabolism; D015003:Yeasts
chemical_list: D002135:Calcium-Binding Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; C062748:NSP1 protein, S cerevisiae; C077730:NUP116 protein, S cerevisiae; C093510:NUP159 protein, S cerevisiae; C095897:NUP82 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D013205:Staphylococcal Protein A; C093350:nuclear pore complex protein 98