title: HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation.
abstract: Colorectal carcinoma RKO cells expressing reduced levels of the RNA-binding protein HuR (ASHuR) displayed markedly reduced growth. In synchronous RKO populations, HuR was almost exclusively nuclear during early G(1), increasing in the cytoplasm during late G(1), S and G(2). The expression and half-life of mRNAs encoding cyclins A and B1 similarly increased during S and G(2), then declined, indicating that mRNA stabilization contributed to their cell cycle-regulated expression. In gel-shift assays using radiolabeled cyclin RNA transcripts and RKO protein extracts, only those transcripts corresponding to the 3'-untranslated regions of cyclins A and B1 formed RNA-protein complexes in a cell cycle-dependent fashion. HuR directly bound mRNAs encoding cyclins A and B1, as anti-HuR antibodies supershifted such RNA-protein complexes. Importantly, the expression and half-life of mRNAs encoding cyclins A and B1 were reduced in ASHuR RKO cells. Our results indicate that HuR may play a critical role in cell proliferation, at least in part by mediating cell cycle-dependent stabilization of mRNAs encoding cyclins A and B1.
authors.affiliation: Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD 21224, USA.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: M C
authors.initials: MC
authors.lastname: Caldwell
authors.forename: S
authors.initials: S
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Furneaux
authors.forename: M
authors.initials: M
authors.lastname: Gorospe
mesh_terms: D000954:Antigens, Surface; D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D019925:Cyclin A / Q000235:genetics*; D019926:Cyclin B / Q000235:genetics*; D056744:Cyclin B1; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000954:Antigens, Surface; C534699:CCNB1 protein, human; D019925:Cyclin A; D019926:Cyclin B; D056744:Cyclin B1; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins