Probing ligand-induced conformational changes of human CD38.
The lymphoid surface antigen CD38 is basically a NAD+glycohydrolase, which is also involved in the metabolism of cyclic ADP-ribose. Besides, this ecto-enzyme has potential signalling roles in T- and B-cells. Such multiple functions prompted us to study the molecular dynamics of the CD38 protein and especially the relationship between its ecto-enzymatic active site and its epitope, i.e. the binding site of most known anti-CD38 monoclonal antibodies. Both epitopic and enzymatic sites were shown to be degraded by proteases, such as trypsin or chymotrypsin. This sensitivity was almost entirely suppressed in the presence of substrates or inhibitors. Both sites were also degraded in the presence of reducing agents, as dithiothreitol. Inhibitory ligands induced the same resistance of both sites against reducing attack. The binding of CD38 ligands to the active site triggers therefore conformational changes that shield some backbone bonds and disulfide bridges against, respectively, proteolytic cleavage or reduction. This transconformation was found moreover to irreversibly take place after incubation with substrates such as NAD+ in the presence of dithiothreitol. The epitope remained preserved, while the enzymatic activity was lost. This inactivation probably resulted from the covalent trapping of the catalytically reactive intermediate in the active site (i.e. paracatalytic inactivation). These data have major implications in the knowledge of the CD38 structure, especially with regard to the location of disulfide bridges and their accessibility. Potential consequences of the conformational plasticity of CD38 should also be considered in its physiological functions such as signalling.
Berthelier
V
V
Laboratoire d'Immunologie Cellulaire, UMR 7627 CNRS, Hôpital Pitié-Salpétrière, Paris, France.
Laboureau
J
J
Boulla
G
G
Schuber
F
F
Deterre
P
P
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000737:chemistry*; D001665:Binding Sites; D002384:Catalysis; D003545:Cysteine / Q000378:metabolism; D004220:Disulfides; D004229:Dithiothreitol / Q000494:pharmacology; D000939:Epitopes; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008562:Membrane Glycoproteins; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D014162:Transfection; D014357:Trypsin / Q000494:pharmacology
D015703:Antigens, CD; D000943:Antigens, Differentiation; D004220:Disulfides; D000939:Epitopes; D008024:Ligands; D008562:Membrane Glycoproteins; D009243:NAD; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1; D014357:Trypsin; D003545:Cysteine; D004229:Dithiothreitol