[Determination and significance of nitric oxide and tumor necrosis factor-alpha induced by peripheral blood monocyte in vitro in patients with malignant bone tumors].
Peripheral blood monocytes from 15 cases of malignant bone tumors and 12 cases of health volunteers were seperated and induced by lipopolysaccharide(LPS) for 48-hour culture. The supernatant was collected for determining the content of nitrite and tumor necrosis factor-alpha(TNF-alpha) (by Griess and ELISA methods, respectively). The results were as follows: The content of nitrite and TNF-alpha in patients with malignant bone tumors was significantly higher than that in normal control; the content of nitrite and TNF-alpha in the malignant bone tumor group presented the positive correlation (r = 0.8909, P < 0.01). The results suggest that NO and TNF-alpha produced by activated macrophages may be related to the pathogenesis of malignant bone tumors.
Xiao
T
T
Department of Surgery, Second Hospital, Hunan Medical University, Changsha.
Liao
L
L
Shun
C
C
Wang
W
W
Li
H
H
Fang
J
J
D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000097:blood*; D002478:Cells, Cultured; D002648:Child; D005260:Female; D018219:Histiocytoma, Benign Fibrous / Q000097:blood; D006801:Humans; D008070:Lipopolysaccharides; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood*; D012516:Osteosarcoma / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide