A simplified procedure for rapid screening of vibrios for cholera toxin production.
Conventional methods for the detection of cholera toxin (CT) production by vibrios are not readily available to most laboratories. A modification is described here of a simplified method standardised earlier and based on the degradation of nicotinamide adenine dinucleotide (NAD) by CT; this is simple and can be carried out in small laboratories also. It is also easy to perform, and gives reproducible results.
Jesudason
M V
MV
Department of Microbiology, Christian Medical College & Hospital, Vellore.
Kuruvilla
A
A
D002772:Cholera Toxin / Q000096:biosynthesis*; D005759:Gastroenteritis / Q000382:microbiology; D006801:Humans; D008828:Microbiological Techniques; D015203:Reproducibility of Results; D013045:Species Specificity; D014733:Vibrio / Q000378:metabolism*
D002772:Cholera Toxin