Detection of a spontaneous high expression of heat shock protein 70 in developing zebrafish (Danio rerio).
A spontaneous high expression of heat shock protein 70 (HSP 70) was detected in zebrafish (Danio rerio) at early larval stage (84 h after fertilization), but the HSP 70 level was either low or barely detectable in 12, 24, 36, 60, and 108 h after fertilization. The extracts of zebrafish at 80 and 84 h after fertilization formed a clear protein-DNA complex with a probe containing heat shock elements (HSEs), suggesting that this spontaneous expression of HSP 70 may be turned on via the binding of stage-specific HSE-binding factors to HSP 70 gene promotor. The protein-HSE complexes produced by the spontaneous binding, however, were found to be different from those formed by the extracts of heat-treated zebrafish in electrophoretic mobility.
Yeh
F L
FL
Institute of Marine Biotechnology, National Taiwan Ocean University, Keelung, Republic of China.
Hsu
T
T
D000818:Animals; D005260:Female; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D008297:Male; D015027:Zebrafish
D018840:HSP70 Heat-Shock Proteins