Resistance of vaccinia virus to inactivation by solvent/detergent treatment of blood products.
The inactivation of enveloped viruses by two different solvent/detergent combinations, i.e. tri-n-butyl phosphate (TNBP)/Triton X-100 or TNBP/Tween 80, has been investigated using a high purity factor VIII (Replenate) and factor IX (Replenine) respectively. Treatment with TNBP/Triton X-100 rapidly inactivated all the typical enveloped viruses tested, i.e. Sindbis, semliki forest virus (SFV), herpes simplex virus type-1 (HSV-1) and vesicular stomatitis virus (VSV), by 3.7-5.8 log within 15 seconds. While virus inactivation with TNBP/Tween 80 was slower, effective inactivation of Sindbis, HSV-1, VSV and human immunodeficiency virus type-1, i.e. 4.1-->6.3 log, occurred within 30 minutes. In contrast, vaccinia virus was relatively resistant to inactivation in either of these solvent/detergent combinations. Incubation times of 10 minutes for TNBP/Triton X-100 or 6-24 hours for TNBP/Tween 80, were required to reach inactivation levels of about 4 log.
Roberts
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Bio Products Laboratory, Elstree, Hertfordshire, UK.
D000818:Animals; D001802:Blood Substitutes / Q000592:standards*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003257:Consumer Product Safety; D006224:Cricetinae; D003902:Detergents / Q000494:pharmacology; D004340:Drug Contamination; D004352:Drug Resistance, Microbial; D005164:Factor IX; D005169:Factor VIII; D006801:Humans; D017830:Octoxynol / Q000494:pharmacology*; D010755:Organophosphates / Q000494:pharmacology*; D012997:Solvents / Q000494:pharmacology; D014616:Vaccinia virus / Q000187:drug effects*; D014709:Vero Cells
D001802:Blood Substitutes; D003902:Detergents; D010755:Organophosphates; D012997:Solvents; D005169:Factor VIII; D005164:Factor IX; D017830:Octoxynol; C009524:tributyl phosphate