title: Acellular bladder matrix allografts in the regeneration of functional bladders: evaluation of large-segment (> 24 cm) substitution in a porcine model.
abstract: OBJECTIVE
  To evaluate the use of a large-segment (> 24 cm2) bladder substitution with porcine bladder acellular matrix allograft (BAMA) in a large animal model. Materials and methods Bladders were harvested from pigs at the time of necropsy and subjected to detergent and enzymatic extractions to render them acellular. The BAMA produced had the surgical handling and suture-retaining properties of normal bladder tissue. Six pigs had BAMA segments implanted under general anaesthesia, through a low midline abdominal incision and after partial cystectomy. The defect was repaired with a BAMA patch (mean size 43.88 cm2, range 12-72), with no urinary diversion. Two animals each were then killed at 9, 16 and 30 days and the bladders explanted. The native bladder and BAMA patch were analysed morphometrically to evaluate cellular re-population and matrix re-organization.
  
  
  RESULTS
  All animals survived surgery; there were no urinary leaks and no stones detected in any of the bladders. At 9 days there was a diffuse infiltration with acute inflammatory cells, but no areas of necrosis. There were isolated areas of smooth muscle cell (SMC) infiltration of the BAMA. At 16 days the luminal surface was lined with a single layer of urothelium, there was stromal infiltration with disorganized SMC and angiogenesis, with mature vessels in the BAMA patch. At 30 days the urothelium was multilayered with organizing groups of SMCs and angiogenesis. The highest cell density was at the periphery of the repopulated BAMA patch, decreasing towards the centre.
  
  
  CONCLUSIONS
  The implantation of large patches of BAMA is technically feasible and may prove to be a viable surgical alternative to bladder augmentation with intestinal segments. The advantages of BAMA include the potential for complete and functional regeneration of a bladder substitute. This model provides a tool with which to obtain a better understanding of the cellular and molecular aspects of matrix re-population.
authors.affiliation: Division of Urology, Department of Cellular and Molecular Pathology and Department of Chemical Engineering, The Hospital for Sick Children, University of Toronto, Toronto, Ontario Canada.
authors.forename: P A
authors.initials: PA
authors.lastname: Merguerian
authors.forename: P P
authors.initials: PP
authors.lastname: Reddy
authors.forename: D J
authors.initials: DJ
authors.lastname: Barrieras
authors.forename: G J
authors.initials: GJ
authors.lastname: Wilson
authors.forename: K
authors.initials: K
authors.lastname: Woodhouse
authors.forename: D J
authors.initials: DJ
authors.lastname: Bagli
authors.forename: G A
authors.initials: GA
authors.lastname: McLorie
authors.forename: A E
authors.initials: AE
authors.lastname: Khoury
mesh_terms: D000818:Animals; D005260:Female; D013552:Swine; D014184:Transplantation, Homologous; D001743:Urinary Bladder / Q000166:cytology* / Q000502:physiology / Q000601:surgery; D016476:Urinary Reservoirs, Continent / Q000473:pathology*; D019459:Urothelium / Q000166:cytology