Inducible protective processes in animal systems VI. Cross-adaptation and the influence of caffeine on the adaptive response in bone marrow cells of mouse.
The effect of caffeine (CAF) (a replicative DNA synthesis inhibitor) given as pre-, inter- and post-treatments on the ethyl methanesulfonate (EMS)-induced adaptive response in in vivo mouse bone marrow cells was studied in order to understand the influence of CAF on the adaptive response. The pre-treatment was given 4 h before a combined treatment with EMS (conditioning + challenge) and in another set CAF was given as a conditioning dose and 4 h later the cells were challenged with a high dose of EMS. In the inter-treatment, CAF (40 mg/kg body wt) was administered 2 or 4 h after the conditioning dose of EMS and 6 or 4 h later the cells were challenged with a high dose of EMS. Similarly, in the post-treatment experiments, CAF was injected 6, 12 or 18 h after a combined treatment with EMS. The results revealed that the pre-, inter- and post-treatments with CAF significantly reduced the frequency of chromosomal aberrations compared with the challenge and combined treatments with EMS. It is interesting to note that CAF pre-treatment resulted in a much greater reduction in chromosomal aberrations compared with the inter- and post-treatments. Thus, this is an example of cross-adaptation induced by CAF in EMS-treated in vivo mouse bone marrow cells and the results also demonstrate an influence of CAF on the adaptive response.
Harish
S K
SK
Department of Zoology, University of Mysore, Manasagangotri, Mysore-570 006 and Department of Life Science, Kuvempu University, Shankarghatta-577 451, Shimoga, India.
Guruprasad
K P
KP
Mahmood
R
R
Vasudev
V
V
D000064:Acclimatization; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002110:Caffeine / Q000008:administration & dosage / Q000494:pharmacology*; D002869:Chromosome Aberrations; D004249:DNA Damage; D005020:Ethyl Methanesulfonate / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D009153:Mutagens; D002110:Caffeine; D005020:Ethyl Methanesulfonate