The fate of adoptively transferred quiescent encephalitogenic T cells in normal and antigen-tolerized mice.
Adoptive transfer of quiescent encephalitogenic T cells to normal syngeneic recipients was without clinical effect. RT-PCR was used to assess localization of an adoptively transferred quiescent encephalitogenic T cell clone in normal and antigen-unresponsive mice prior to or after challenge with neuroantigen/CFA. The T cell clone was not detectable in lymphoid tissues prior to challenge with neuroantigen; however, following challenge, the clone was found in the spleen, lymph nodes and spinal cord of both normal and antigen-tolerized mice. The latter animals remained clinically normal. Non-activated encephalitogenic T cells transferred to wild-type recipients pretreated i.p. with neuroantigen/IFA were rendered unresponsive. Transfer of the same T cells to alpha/beta T cell-deficient mice pretreated with neuroantigen/IFA resulted in spontaneous disease indicating that an intact alpha/beta T cell system was required for development of the unresponsive state.
Fritz
R B
RB
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. rfritz@mcw.edu
Wang
X
X
Zhao
M L
ML
D019264:Adoptive Transfer; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002478:Cells, Cultured; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000473:pathology; D005260:Female; D005620:Freund's Adjuvant / Q000737:chemistry / Q000276:immunology; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D004676:Myelin Basic Protein / Q000378:metabolism; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000172:deficiency; D012016:Reference Values; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology / Q000637:transplantation
D000941:Antigens; D004676:Myelin Basic Protein; D016693:Receptors, Antigen, T-Cell, alpha-beta; D005620:Freund's Adjuvant