Hydrophobic displacement chromatography of proteins.
Displacement chromatography of proteins was successfully carried out in both hydrophobic interaction and reversed-phase chromatographic systems using low-molecular weight displacers. The displacers employed for hydrophobic displacement chromatography were water soluble, charged molecules containing several short alkyl and/or aryl groups. Spectroscopy was employed to verify the absence of structural changes to the proteins displaced on these hydrophobic supports. Displacement chromatography on a reversed-phase material was employed to purify a growth factor protein from its closely related variants, demonstrating the high resolutions that can be achieved by hydrophobic displacement chromatography. This process combines the high-resolution/high-throughput characteristics of displacement chromatography with the unique selectivity of these hydrophobic supports and offers the chromatographic engineer a powerful tool for the preparative purification of proteins.
Shukla
A A
AA
ICOS Corporation, 22021, 20th Avenue SE, Bothell, Washington 98021, USA.
Sunasara
K M
KM
Rupp
R G
RG
Cramer
S M
SM
D001709:Biotechnology; D019208:Brain-Derived Neurotrophic Factor / Q000737:chemistry / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
D019208:Brain-Derived Neurotrophic Factor; D007202:Indicators and Reagents; D011506:Proteins; D011994:Recombinant Proteins