Frequent gain of the p40/p51/p63 gene locus in primary head and neck squamous cell carcinoma.
We have identified a new human p53 homologue, p40 (p51/p63). This gene was mapped to the distal arm of 3q and was found to be essential for normal epithelial development. We used microsatellite and FISH analyses to search for genetic alterations of p40 in primary HNSCC. A more precise localization of p40 was completed using 6 known markers on 3q and a newly isolated microsatellite marker within the p40 gene. We also determined the genomic organization of the p40 gene using human YAC and BAC clones. Microsatellite analysis revealed that 14 of 26 (54%) primary HNSCC had allelic imbalance in at least 1 of the 7 microsatellite loci. However, FISH analysis with a p40 probe showed that a majority of HNSCC had an increased copy number of the locus regardless of allelic status. Thus, overrepresentation of the p40 locus may play an important role in the development of HNSCC.
Yamaguchi
K
K
Division of Head and Neck Cancer Research, Department of Otolaryngology, Head and Neck Surgery, The Johns Hopkins University, Baltimore, Maryland 21205-2196, USA.
Wu
L
L
Caballero
O L
OL
Hibi
K
K
Trink
B
B
Resto
V
V
Cairns
P
P
Okami
K
K
Koch
W M
WM
Sidransky
D
D
Jen
J
J
D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D005091:Exons / Q000235:genetics; D016147:Genes, Tumor Suppressor; D015894:Genome, Human; D006258:Head and Neck Neoplasms / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D008565:Membrane Proteins; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D019255:NADPH Oxidases; D010750:Phosphoproteins / Q000235:genetics*; D011506:Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins
C081876:CKAP4 protein, human; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D011506:Proteins; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D019255:NADPH Oxidases; C113333:NCF4 protein, human