Effect of serum withdrawal on the proliferation of B16F10 melanoma cells.
B16F10 murine melanoma cell proliferation was inhibited after 48 h in medium with serum in the range 0.1 to 0.5% by volume. Cell viability was mostly retained, whereas cells completely deprived of serum died. Growth-arrested cultures showed serum-dependent suppression of DNA synthesis. The response was typically that of a 'cell cycle freeze', verified by flow cytometric distribution of cells. Consequently, serum deprivation did not lead to synchrony when serum was restored to arrested populations. Furthermore, there was no change in PCNA expression in arrested cells.
Rodriguez-Ayerbe
C
C
Department of Zoology and Animal Cell Dynamic, Faculty of Science, University of the Basque Country, Bilbao, 48080, Spain.
Smith-Zubiaga
I
I
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen