Plasmodium yoelii: identification of a gene encoding a putative ADP-ribosylation factor-1 GTPase-activating protein, PyAG1.
The PyAG1 gene, identified by the screening of a Plasmodium yoelii genomic DNA library with a rhoptry-specific Mab, encodes a protein with a zinc finger structure immediately followed by the consensus sequence of the Arf GAP catalytic site. The serum of mice immunized with the recombinant protein recognized specifically the rhoptries of the late infected erythrocytic stages. Blast analysis using the Genbank database gave the highest scores with four proteins presenting an Arf1 GAP activity. If presenting also this activity, the PyAG1 protein could be involved in the regulation of the secreted protein vesicular transport and, consequently, in the rhoptry biogenesis.
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Unité de Parasitologie, Institut de Médecine Tropicale, Service de Santé des Armées, Marseille Armées, France. imtssa@gulliver.fr
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Parzy
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Doury
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D020823:ADP-Ribosylation Factor 1 / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005455:Fluorescent Antibody Technique; D020690:GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism; D017125:Genes, Protozoan; D015698:Genomic Library; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010967:Plasmodium yoelii / Q000235:genetics* / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics; D051381:Rats
D020690:GTPase-Activating Proteins; D015800:Protozoan Proteins; D020823:ADP-Ribosylation Factor 1