Tritium sequence analysis of oligoribonucleotides: a combination of post-labeling and thin-layer chromatographic techniques for the analysis of partial snake venom phosphodiesterase digests.
A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide-3' dialdehydes generated by controlled snake venom phosphodiesterase/alkaline phosphomonoesterase digestion and periodate treatment of time point aliquots of the incubation mixture. Radioactive oligonucleotide derivatives are resolved according to chain length by PEI-cellulose(1) anion-exchange TLC and their 3'-termini identified by techniques described in the preceding paper of this series(2). The present tritium derivative method is compared with the one described previously(2).
Sivarajan
M
M
Gupta
R C
RC
Chia
L L
LL
Randerath
E
E
Randerath
K
K
D001894:Borohydrides / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004722:Endoribonucleases / Q000378:metabolism; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000494:pharmacology*; D017423:Sequence Analysis, RNA / Q000379:methods*; D014316:Tritium / Q000737:chemistry*
D001894:Borohydrides; D014316:Tritium; D010504:Periodic Acid; C025364:sodium borohydride; C009288:metaperiodate; D004722:Endoribonucleases; C022543:ribonuclease T(2); D010744:Phosphoric Monoester Hydrolases; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom