title: Vascular structure and microcirculation of experimental pancreatic carcinoma in rats.
abstract: OBJECTIVE
  To study angiogenesis and microcirculation in experimental pancreatic carcinoma.
  
  
  METHODS
  Open experimental study.
  
  
  METHODS
  2 University hospitals, Germany and USA.
  
  
  METHODS
  16 male Lewis rats.
  
  
  METHODS
  Induction of a duct-like pancreatic cancer in the pancreas and peritoneum by interposition of fragments of tumour between 2 inert transparent polymethylmethacrylate plates.
  
  
  METHODS
  Microcirculation in the tumour and interaction between leucocytes, tumour, and endothelium investigated by intravital microscopy.
  
  
  RESULTS
  The density of vessels in the carcinoma was significantly less than in normal pancreatic tissue (p = 0.0004). The vasculature of the tumour was characterised by a lack of differentiation in architecture of vessels, formation of sinusoidal and lacunar vessels and sudden changes in diameter of vessels. Red blood cell velocity differed among tumour vessels, but mean values were similar to those of normal exocrine pancreas. The mixed lymphocyte culture test indicated that the cell line DSL6A was immunogenic. However, high-affinity leucocyte-endothelium-interaction was significantly reduced in the tumour's microcirculation after both orthotopic and heterotopic implantation (p = 0.002). Rates of apoptosis were suppressed in heterotopic tumours compared with orthotopic ones. Tumour growth was faster in heterotopic tumours.
  
  
  CONCLUSIONS
  Experimental duct-like pancreatic carcinoma can be differentiated from normal pancreas by: chaotic arrangement of vessels with loss of differentiation of architecture and heterogeneous distribution; the formation of sinusoidal or lacunar vessels; lower vascular density with similar erythrocyte velocity; increase in mean diameter of vessels; reduced leucocyte-endothelium interaction despite confirmed immunogeneity independent of wall shear rates. The site of implantation influences apoptosis and growth rates.
authors.affiliation: Department of Surgery, University of Heidelberg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schmidt
authors.forename: E
authors.initials: E
authors.lastname: Ryschich
authors.forename: V
authors.initials: V
authors.lastname: Daniel
authors.forename: L
authors.initials: L
authors.lastname: Herzog
authors.forename: J
authors.initials: J
authors.lastname: Werner
authors.forename: C
authors.initials: C
authors.lastname: Herfarth
authors.forename: D S
authors.initials: DS
authors.lastname: Longnecker
authors.forename: M M
authors.initials: MM
authors.lastname: Gebhard
authors.forename: E
authors.initials: E
authors.lastname: Klar
mesh_terms: D000818:Animals; D007962:Leukocytes / Q000502:physiology; D007959:Lymphocyte Culture Test, Mixed; D008833:Microcirculation; D009389:Neovascularization, Pathologic; D010190:Pancreatic Neoplasms / Q000098:blood supply / Q000473:pathology* / Q000503:physiopathology*; D019904:Polymethyl Methacrylate; D051381:Rats; D011917:Rats, Inbred Lew; D014407:Tumor Cells, Cultured
chemical_list: D019904:Polymethyl Methacrylate