The digestive cells of the hepatopancreas in Aplysia depilans (Mollusca, Opisthobranchia): ultrastructural and cytochemical study.
Digestive cells are the most abundant cell type in the digestive diverticula of Aplysia depilans. These are tall columnar or club shaped cells, covered with microvilli on their apical surface. A large number of endocytic vesicles containing electron-dense substances can be found in the apical zone, but the presence of many heterolysosomes of large diameter is the main feature of these cells. Glycogen particles and some lipid droplets were also observed. Peroxisomes with a circular or oval profile were common, but crystalline nucleoids were not detected in them, although a dense spot in the matrix was observed in a few cases. These organelles were strongly stained after cytochemical detection of catalase activity. The Golgi stacks are formed by 4 or 5 cisternae, with dilated zones containing electron dense material. Arylsulphatase activity was detected in the Golgi stacks and also in lysosomes. Cells almost entirely occupied by a very large vacuole containing a residual dense mass seem to be digestive cells in advanced stages of maturation. The observation of semithin and ultrathin sections indicates that these very large vacuoles are the result of a fusion among the smaller lysosomes. Some images suggest that the content of these large vacuoles is extruded into the lumen of the digestive diverticula.
Lobo-da-Cunha
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Laboratory of Cell Biology, Institute of Biomedical Sciences and CIMAR, Oporto University, Portugal. alcunha@icbas.up.pt
D000818:Animals; D001048:Aplysia / Q000166:cytology* / Q000648:ultrastructure; D001192:Arylsulfatases / Q000032:analysis; D002374:Catalase / Q000032:analysis; D004064:Digestive System / Q000166:cytology* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000648:ultrastructure; D006651:Histocytochemistry; D008247:Lysosomes / Q000201:enzymology / Q000648:ultrastructure; D008854:Microscopy, Electron; D015388:Organelles / Q000201:enzymology / Q000648:ultrastructure; D020675:Peroxisomes / Q000201:enzymology / Q000648:ultrastructure
D002374:Catalase; D001192:Arylsulfatases