Glucose phosphorylation capacity and glycolysis in the liver of arthritic rats.
OBJECTIVE
Glycolysis and the glucose phosphorylation capacity of livers from arthritic rats were studied because alterations in these parameters are suggested by some studies.


METHODS
Arthritis was induced in male albino rats (Wistar; 180-220 g).


METHODS
The animals were injected with 100 microl heat inactivated Mycobacterium tuberculosis suspended in mineral oil at a concentration of 0.5% (w/v). Animals showing lesions after 14 to 21 days were selected.


METHODS
Glucose phosphorylation was measured in the high speed supernatant fraction of liver homogenates and glycolysis in the isolated perfused liver.


RESULTS
The glucose concentration for half-maximal rates was reduced from 18.32+/-5.69 in normal to 9.84+/-3.15 mM in arthritic rats (p = 0.024). Vmax was increased from 8.77+/-0.27 in normal to 11.49+/-0.29 nmol min(-1) mg protein(-1) in arthritic rats (p = 0.001). Perfused livers from arthritic rats showed a 2.43-fold higher rate of glycolysis.


CONCLUSIONS
Livers from arthritic rats present a higher glucose phosphorylation capacity. Possibly this phenomenon is caused by circulating inflammatory mediators produced during adjuvant-induced arthritis.
Fedatto
Z
Z
Laboratory of Liver Metabolism, University of Maringá, Brazil.
Ishii-Iwamoto
E L
EL
Bersani-Amado
C
C
Maciel
E R
ER
Bracht
A
A
Kelmer-Bracht
A M
AM
D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar
D005947:Glucose