Determination of lipoprotein(a) concentrations and apolipoprotein(a) molecular weights in diabetic patients.
Lipoprotein(a) (Lp(a)) with atherogenic and thrombotic properties has been frequently studied in diabetes, because a high cardiovascular risk has been reported both in type 1 and type 2 diabetes. Few studies have considered genetic factors, especially the isoforms of apolipoprotein(a). The aim of this work is to determine the distribution of apo(a) phenotypes in the serum of 148 diabetic patients (59 type 1, 89 type 2) with or without vascular complications. Apo(a) phenotypes are determined using 4-15% sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by immunoblotting (PhastSystem - Pharmacia). An inverse relationship is observed between Lp(a) serum concentration and the apparent molecular mass of apo(a) isoforms: type 1 r=- 0.61, p<0.01; type 2 r=- 0.55, p<0.01. The frequency of apo(a) isoforms is significantly different between type 1 and type 2 diabetes mellitus. A higher prevalence of isoforms of low molecular weight was observed in the type 2 diabetic population.
Ribault
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Laboratoire de Biochimie, Hôpital Sud, 16 boulevard de Bulgarie, B. P. 56129, 35056 Rennes Cedex 2, France. Annie.Ruelland@univ-rennes1.fr
Durou
M R
MR
Letellier
C
C
Wojcik
F
F
Poirier
J Y
JY
Ruelland
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A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001053:Apolipoproteins / Q000097:blood / Q000737:chemistry* / Q000235:genetics; D053241:Apoprotein(a); D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D015151:Immunoblotting; D017270:Lipoprotein(a) / Q000097:blood* / Q000737:chemistry* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010641:Phenotype; D020033:Protein Isoforms / Q000097:blood / Q000737:chemistry / Q000235:genetics; D012016:Reference Values
D001053:Apolipoproteins; D017270:Lipoprotein(a); D020033:Protein Isoforms; D053241:Apoprotein(a)