title: In vitro susceptibility tests for cationic peptides: comparison of broth microdilution methods for bacteria that grow aerobically.
abstract: The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria that grow aerobically, while the second was performed according to the procedures recently proposed by the R. E. W. Hancock laboratory for testing antimicrobial peptides. Overall, the first method produced MICs two- and fourfold higher than the second method.
authors.affiliation: Institute of Infectious Diseases and Public Health, University of Ancona, Italy. cmalinf@popcsi.unian.it
authors.forename: A
authors.initials: A
authors.lastname: Giacometti
authors.forename: O
authors.initials: O
authors.lastname: Cirioni
authors.forename: F
authors.initials: F
authors.lastname: Barchiesi
authors.forename: M S
authors.initials: MS
authors.lastname: Del Prete
authors.forename: M
authors.initials: M
authors.lastname: Fortuna
authors.forename: F
authors.initials: F
authors.lastname: Caselli
authors.forename: G
authors.initials: G
authors.lastname: Scalise
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D054806:Magainins; D009561:Nisin / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology; D029867:Xenopus Proteins
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; D010455:Peptides; D010456:Peptides, Cyclic; D011506:Proteins; D029867:Xenopus Proteins; C097849:buforin II; C073143:indolicidin; C053301:magainin 2 peptide, Xenopus; D009561:Nisin; C086618:ranalexin; C070016:cecropin P1-LI