title: Natural caprine herpesvirus 1 (CpHV-1) infection in kids.
abstract: Twenty-seven kids aged 5-7 days from a flock of 200 goats in which a high rate of abortion occurred died over a 2-month period. All showed hyperthermia, abdominal pain and anorexia. Two of the kids were examined post mortem. Ulcerative and necrotic lesions affected the whole intestine, and macroscopical changes were also observed in the lungs, urinary bladder and liver. Histologically, a severe necrotizing enteritis as well as thickening of the alveolar septa and necrotic bronchiolo-alveolitis were detected. Prominent microscopical lesions were also present in the liver, urinary bladder, spleen, thymus, mesenteric lymph nodes and kidney. Macrophages containing eosinophilic intranuclear inclusion bodies appeared to be the main inflammatory cell in all the organs examined. Ultrastructurally, herpesvirus particles were evident. Characteristic morphological features of type-A capsids (empty), type-C capsids (large core), and type-B capsids were observed. A virus was isolated in cell culture from all organs examined in the two kids. Cytopathogenic effects (rounding cells, syncytia, vacuoles, cell lysis) and acidophilic intranuclear inclusions typical of herpesvirus infection were observed. The virus was identified as caprine herpesvirus 1 (CpHV-1) by the polymerase chain reaction (PCR) technique and by serum neutralization
authors.affiliation: Department of Pathology and Animal Health, Naples University "Federico II", via Veterinaria 1, Naples, 80137, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Roperto
authors.forename: A
authors.initials: A
authors.lastname: Pratelli
authors.forename: G
authors.initials: G
authors.lastname: Guarino
authors.forename: V
authors.initials: V
authors.lastname: Ambrosio
authors.forename: M
authors.initials: M
authors.lastname: Tempesta
authors.forename: P
authors.initials: P
authors.lastname: Galati
authors.forename: G
authors.initials: G
authors.lastname: Iovane
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D018138:Alphaherpesvirinae / Q000302:isolation & purification*; D000818:Animals; D015511:Goat Diseases / Q000473:pathology / Q000821:virology*; D006041:Goats; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology