Suppression of facilitative glucose transporter 1 mRNA can suppress tumor growth.
We attempted to suppress glucose transporter 1 (GLUT1) expression by transfecting MKN45 cells with cDNA for antisense GLUT1. Glucose transport was significantly decreased in cells with antisense GLUT1 compared with wild-type cells or cells with vector alone. Suppression of GLUT1 mRNA resulted in a decreased number of cells in the S phase. This was accompanied by overexpression of p21 protein. Tumorigenicity in the nude mice injected with antisense GLUT1 expressing cells was significantly slower than in those with wild-type MKN45 cells. These results suggest that antisense GLUT1 mRNA inhibits tumor growth through a G(1) arrest and that expression of antisense GLUT1 mRNA via gene therapy can be used as a tool in the treatment of cancer.
Noguchi
Y
Y
First Department of Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Japan. yznog@med.yokohama-cu.ac.jp
Saito
A
A
Miyagi
Y
Y
Yamanaka
S
S
Marat
D
D
Doi
C
C
Yoshikawa
T
T
Tsuburaya
A
A
Ito
T
T
Satoh
S
S
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D001692:Biological Transport / Q000235:genetics; D053938:DNA Fragmentation; D016373:DNA, Antisense / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D016193:G1 Phase; D051272:Glucose Transporter Type 1; D007700:Kinetics; D051379:Mice; D008813:Mice, Inbred ICR; D008819:Mice, Nude; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016196:S Phase; D013274:Stomach Neoplasms / Q000378:metabolism; D013489:Suppression, Genetic; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D018076:DNA, Complementary; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495678:Slc2a1 protein, mouse; D016283:Proto-Oncogene Proteins p21(ras)