title: Development and characterization of a cell line from Pacific herring, Clupea harengus pallasi, sensitive to both naphthalene cytotoxicity and infection by viral hemorrhagic septicemia virus.
abstract: A cell line, PHL, has been successfully established from newly hatched herring larvae. The cells are maintained in growth medium consisting of Leibovitz's L-15 supplemented with 15% fetal bovine serum (FBS), and have been cryopreserved and maintain viability after thawing. These cells retain a diploid karotype after 65 population doublings. PHL are susceptible to infection by the North American strain of viral hemorrhagic septicemia (VHS) virus, and are sensitive to the cytotoxic effects of naphthalene, a common environmental contaminant. Naphthalene is a component of crude and refined oil, and may be found in the marine environment following acute events such as oil spills. In addition, chronic sources of naphthalene contamination include offshore drilling and petroleum contamination from areas such as docks and marinas that have creosote-treated docks and pilings and also receive constant small inputs of petroleum products. This cell line should be useful for investigations of the toxicity of naphthalene and other petroleum components to juvenile herring. In addition, studies of the VHS virus will be facilitated by the availability of a susceptible cell line from an alternative species.
authors.affiliation: Bamfield Marine Station, British Columbia, Canada.
authors.forename: R C
authors.initials: RC
authors.lastname: Ganassin
authors.forename: S M
authors.initials: SM
authors.lastname: Sanders
authors.forename: C J
authors.initials: CJ
authors.lastname: Kennedy
authors.forename: E M
authors.initials: EM
authors.lastname: Joyce
authors.forename: N C
authors.initials: NC
authors.lastname: Bols
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D002875:Chromosomes; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005326:Fetal Proteins / Q000494:pharmacology; D005393:Fish Diseases / Q000139:chemically induced / Q000821:virology*; D005399:Fishes; D005648:Fuel Oils; D006470:Hemorrhage / Q000821:virology; D007814:Larva / Q000166:cytology / Q000201:enzymology; D009281:Naphthalenes / Q000633:toxicity*; D010088:Oxidoreductases / Q000378:metabolism; D012209:Rhabdoviridae; D018353:Rhabdoviridae Infections / Q000503:physiopathology*; D018805:Sepsis / Q000821:virology; D013696:Temperature; D014876:Water Pollution
chemical_list: D005326:Fetal Proteins; D005648:Fuel Oils; D009281:Naphthalenes; C031721:naphthalene; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; C076307:methoxyresorufin-O-demethylase; D019363:Cytochrome P-450 CYP1A1