title: Synthesis, 1H NMR structure, and activity of a three-disulfide-bridged maurotoxin analog designed to restore the consensus motif of scorpion toxins.
abstract: Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus. The toxin displays an exceptionally wide range of pharmacological activity since it binds onto small conductance Ca(2+)-activated K(+) channels and also blocks Kv channels (Shaker, Kv1.2 and Kv1.3). MTX possesses 53-68% sequence identity with HsTx1 and Pi1, two other K(+) channel short chain scorpion toxins cross-linked by four disulfide bridges. These three toxins differ from other K(+)/Cl(-)/Na(+) channel scorpion toxins cross-linked by either three or four disulfide bridges by the presence of an extra half-cystine residue in the middle of a consensus sequence generally associated with the formation of an alpha/beta scaffold (an alpha-helix connected to an antiparallel beta-sheet by two disulfide bridges). Because MTX exhibits an uncommon disulfide bridge organization among known scorpion toxins (C1-C5, C2-C6, C3-C4, and C7-C8 instead of C1-C4, C2-C5, and C3-C6 for three-disulfide-bridged toxins or C1-C5, C2-C6, C3-C7, and C4-C8 for four-disulfide-bridged toxins), we designed and chemically synthesized an MTX analog with three instead of four disulfide bridges ([Abu(19),Abu(34)]MTX) and in which the entire consensus motif of scorpion toxins was restored by the substitution of the two half-cystines in positions 19 and 34 (corresponding to C4 and C8) by two isosteric alpha-aminobutyrate (Abu) derivatives. The three-dimensional structure of [Abu(19), Abu(34)]MTX in solution was solved by (1)H NMR. This analog adopts the alpha/beta scaffold with now conventional half-cystine pairings connecting C1-C5, C2-C6, and C3-C7 (with C4 and C8 replaced by Abu derivatives). This novel arrangement in half-cystine pairings that concerns the last disulfide bridge results mainly in a reorientation of the alpha-helix regarding the beta-sheet structure. In vivo, [Abu(19),Abu(34)]MTX remains lethal in mice as assessed by intracerebroventricular injection of the peptide (LD(50) value of 0. 25 microg/mouse). The structural variations are also accompanied by changes in the pharmacological selectivity of the peptide, suggesting that the organization pattern of disulfide bridges should affect the three-dimensional presentation of certain key residues critical to the blockage of K(+) channel subtypes.
authors.affiliation: Laboratoire de Biochimie, CNRS Unité Mixte de Recherche 6560, IFR Jean Roche, Faculté de Médecine Nord, Boulevard Pierre Dramard, 13916, Marseille Cédex 20, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Fajloun
authors.forename: G
authors.initials: G
authors.lastname: Ferrat
authors.forename: E
authors.initials: E
authors.lastname: Carlier
authors.forename: M
authors.initials: M
authors.lastname: Fathallah
authors.forename: C
authors.initials: C
authors.lastname: Lecomte
authors.forename: G
authors.initials: G
authors.lastname: Sandoz
authors.forename: E
authors.initials: E
authors.lastname: di Luccio
authors.forename: K
authors.initials: K
authors.lastname: Mabrouk
authors.forename: C
authors.initials: C
authors.lastname: Legros
authors.forename: H
authors.initials: H
authors.lastname: Darbon
authors.forename: H
authors.initials: H
authors.lastname: Rochat
authors.forename: J M
authors.initials: JM
authors.lastname: Sabatier
authors.forename: M
authors.initials: M
authors.lastname: De Waard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004220:Disulfides; D015195:Drug Design; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012604:Scorpion Venoms / Q000737:chemistry* / Q000235:genetics; D012605:Scorpions; D014118:Toxins, Biological / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics
chemical_list: D004220:Disulfides; D012604:Scorpion Venoms; D014118:Toxins, Biological; C104498:maurotoxin