title: The effect of cisapride on total parenteral nutrition-associated cholestasis in rats.
abstract: BACKGROUND
  Cholestasis is a frequent problem in patients on total parenteral nutrition. Cisapride has a prokinetic effect on the biliary system, but its effect on hepatic excretory function is unknown.
  
  
  OBJECTIVE
  To study the effect of cisapride on TPN-induced cholestasis in a rat model.
  
  
  METHODS
  Bile flow and bile salt secretion rate were measured in rats given TPN. There were four groups of 8 to 13 animals each. After a one hour baseline period during which all four groups received i.v. saline infusion, two groups received a TPN solution for another 2 hours, while saline was infused in the two control groups. At the beginning of the second hour, 2 mg/kg cisapride was injected i.v. as a bolus into one experimental and one control group. Bile was collected from the common bile duct.
  
  
  RESULTS
  At the end of the third hour, TPN caused a significant reduction in bile flow (P < 0.02) and bile salt secretion rate (P < 0.001) (61.24 vs. 50.74 microliters/min/kg, and 1.173 vs. 0.799 mumol/min/kg, respectively). Addition of cisapride abolished the cholestatic effect of TPN.
  
  
  CONCLUSIONS
  Cisapride has a protective effect against TPN-associated cholestasis. This may have clinical significance, and further studies are warranted.
authors.affiliation: Institute of Gastroenterology and Nutrition, Schneider Children's Medical Center of Israel, Petah Tiqva, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Zahavi
authors.forename: O
authors.initials: O
authors.lastname: Rosezki
authors.forename: Y
authors.initials: Y
authors.lastname: Stolkarts
authors.forename: R
authors.initials: R
authors.lastname: Shamir
authors.forename: B
authors.initials: B
authors.lastname: Heckelman
authors.forename: H
authors.initials: H
authors.lastname: Marcus
authors.forename: G
authors.initials: G
authors.lastname: Dinari
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002779:Cholestasis / Q000209:etiology / Q000517:prevention & control*; D020117:Cisapride / Q000627:therapeutic use*; D005765:Gastrointestinal Agents / Q000627:therapeutic use*; D008297:Male; D010288:Parenteral Nutrition / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001647:Bile Acids and Salts; D005765:Gastrointestinal Agents; D020117:Cisapride