title: RBE-LET relationships of high-LET radiations in Drosophila mutations.
abstract: The relative biological effectiveness (RBE) of 252Cf neutrons and synchrotron-generated high-energy charged particles for mutation induction was evaluated as a function of linear energy transfer (LET), using the loss of heterozygosity for wing-hair mutations and the reversion of the mutant white-ivory eye-color in Drosophila melanogaster. Loss of heterozygosity for wing-hair mutations results predominantly from mitotic crossing over induced in wing anlage cells of larvae, while the reverse mutation of eye-color is due to an intragenic structural change (2.96 kb-DNA excision) in the white locus on the X-chromosome. The measurements were performed in a combined mutation assay system so that induced mutant wing-hair clones as well as revertant eye-color clone can be detected simultaneously in the same individual. Larvae were irradiated at the age of 3 days post oviposition with 252Cf neutrons, carbon beam or neon beam. For the neutron irradiation, the RBE values for wing-hair mutations were larger than that for eye-color mutation by about 7 fold. The RBE of carbon ions for producing the wing-hair mutations increased with increase in LET. The estimated RBE values were found to be in the range 2 to 6.5 for the wing-hair. For neon beam irradiation, the RBE values for wing-hair mutations peak near 150 keV/micron and decrease with further increase in LET. On the other hand, the RBE values for the induction of the eye-color mutation are nearly unity in 252Cf neutrons and both ions throughout the LET range irradiated. We discuss the relationships between the initial DNA damage and LET in considering the mechanism of somatic mutation induction.
authors.affiliation: Faculty of Environmental Studies, Nagasaki University, Japan. isao@net.nagasaki-u.ac.jp
authors.forename: I
authors.initials: I
authors.lastname: Yoshikawa
authors.forename: T
authors.initials: T
authors.lastname: Takatsuji
authors.forename: M
authors.initials: M
authors.lastname: Nagano
authors.forename: J
authors.initials: J
authors.lastname: Takada
authors.forename: S
authors.initials: S
authors.lastname: Endo
authors.forename: M
authors.initials: M
authors.lastname: Hoshi
mesh_terms: D000818:Animals; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000235:genetics* / Q000528:radiation effects*; D018499:Linear Energy Transfer; D009030:Mosaicism; D009154:Mutation; D012062:Relative Biological Effectiveness