title: The nucleotide sequence preceding an RNA polymerase initiation site on SV40 DNA. Part 2. The sequence of the early strand transcript.
abstract: The nucleotide sequence of the RNA transcript from the "early" (E) strand of SV40 DNA immediately preceding the preferred E. coli RNA polymerase start site is G-(A-A-A-C, -A-U-)-A-A-A-A-U-G-A-A-U-G-C-A-A-U-U-G-U-U-G-U-U-G-U-U-A-A-C-U-U-G-U-U-U-A-U-U-G-C-A-G-C-U-U-A-U-A-A-U-G-G-U-U-A-C-Ap. The last nucleotide of the sequence is the first nucleotide transcribed by E. coli RNA polymerase from the "E" strand. The DNA template contains a palindrome of 17 residues that includes the Hemophilus influenza restriction endonuclease cleavage site G-T-T-A-A-Cp. The DNA which gives this transcript lies very close to one end of SV40 DNA segment in the Adeno-SV40 hybrid virus Ad2+ND3 and appears to contain sufficient untranscribed information to specify the E. coli RNA polymerase start.
authors.forename: R
authors.initials: R
authors.lastname: Dhar
authors.forename: S M
authors.initials: SM
authors.lastname: Weissman
authors.forename: B S
authors.initials: BS
authors.lastname: Zain
authors.forename: J
authors.initials: J
authors.lastname: Pan
authors.forename: A M
authors.initials: AM
authors.lastname: Lewis
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D006193:Haemophilus influenzae / Q000201:enzymology; D008969:Molecular Sequence Data; D018075:RNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D013539:Simian virus 40 / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D018075:RNA, Complementary; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes