The biologic response to laser thermal modification in an in vivo sheep model.
The purpose of this study was to evaluate the effect of nonablative laser energy on mechanical, histologic, ultrastructural, and biochemical properties of joint capsular tissue in an in vivo sheep model. Femoropatellar joint capsule was treated with the holmium:yttrium-aluminum-garnet laser via an arthroscope, and tissues were harvested immediately after surgery, or at 3, 7, 14, 30, 60, 90, and 180 days after surgery (n = 8/group). Laser treatment caused significant decreases in tissue stiffness from 0 to 7 days after surgery, then stiffness gradually increased after 14 days. Tissue strength was lowest 3 days after laser treatment. Histologic examination revealed immediate collagen hyalinization and cell necrosis, followed by active cellular response characterized by extensive fibroblast migration and capillary sprouting. Tissue appeared to be normal histologically 60 days after surgery; however, collagen fibrils remained uniformly small. This study showed an active tissue response secondary to thermal modification with concomitant recovery of mechanical properties by 30 days after surgery. Whether the shrinkage or joint stability was maintained with time remains to be evaluated. To clarify the advantages and disadvantages of this technique, a carefully controlled clinical trial with long term followup should be performed.
Hayashi
K
K
Comparative Orthopaedic Research Laboratory, School of Veterinary Medicine, University of Wisconsin-Madison, USA.
Hecht
P
P
Thabit
G
G
Peters
D M
DM
Vanderby
R
R
Cooley
A J
AJ
Fanton
G S
GS
Orwin
J F
JF
Markel
M D
MD
D000818:Animals; D001696:Biomechanical Phenomena; D003094:Collagen / Q000378:metabolism; D005260:Female; D007593:Joint Instability / Q000532:radiotherapy*; D007596:Joints / Q000473:pathology / Q000528:radiation effects*; D053685:Laser Therapy; D008854:Microscopy, Electron; D012756:Sheep; D012783:Shoulder Dislocation / Q000532:radiotherapy*; D012785:Shoulder Joint / Q000473:pathology / Q000528:radiation effects
D003094:Collagen