Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance.
The line probe assay (LiPA), a rapid molecular method for detecting rifampicin resistance (RMPr) in Mycobacterium tuberculosis, correctly identified all 145 rifampicin-sensitive (RMPs) and 262 (98.5%) of 266 RMPr strains among 411 isolates collected from diverse countries. If used as a marker of multidrug-resistant tuberculosis (MDR-TB), detection of RMPr by LiPA would have detected 236 of the 240 MDR strains in this study but would have wrongly suggested the presence of MDR in 26 RMP-monoresistant isolates (sensitivity 98.3%, specificity 84.8%). Hence, the reliability of using LiPA (or any other rapid RMPr-detection method) as a surrogate marker of MDR-TB largely depends on the prevalence of RMP-monoresistance in the study population. This approach must therefore be validated in each local situation.
Traore
H
H
Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium. traore@itg.be
Fissette
K
K
Bastian
I
I
Devleeschouwer
M
M
Portaels
F
F
D000349:Africa / Q000453:epidemiology; D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001208:Asia / Q000453:epidemiology; D004351:Drug Resistance; D005060:Europe / Q000453:epidemiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D015336:Molecular Probe Techniques; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013020:South America / Q000453:epidemiology; D013045:Species Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*
D000904:Antibiotics, Antitubercular; D012293:Rifampin