The GCD10 subunit of yeast eIF-3 binds the methyltransferase-like domain of the 126 and 183 kDa replicase proteins of tobacco mosaic virus in the yeast two-hybrid system.
The tobacco mosaic virus (TMV) replicase complex contains virus- and host-encoded proteins. In tomato, one of these host proteins was reported previously to be related serologically to the GCD10 subunit of yeast eIF-3. The yeast two-hybrid system has now been used to show that yeast GCD10 interacts selectively with the methyltransferase domain shared by the 126 and 183 kDa TMV replicase proteins. These findings are consistent with a role for a GCD10-like protein in the TMV replicase complex and suggest that, in TMV-infected cells, the machinery of virus replication and protein synthesis may be closely connected.
Taylor
D N
DN
Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK.
Carr
J P
JP
D039621:Eukaryotic Initiation Factor-3; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014027:Tobacco Mosaic Virus / Q000201:enzymology*; D020798:Two-Hybrid System Techniques; D012359:tRNA Methyltransferases
D039621:Eukaryotic Initiation Factor-3; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C409023:Gcd10 protein, S cerevisiae; D008780:Methyltransferases; D012359:tRNA Methyltransferases; D012324:RNA-Dependent RNA Polymerase