Presence and quantification of neuropeptide Y in pulmonary edema fluids in rats.
The present study was undertaken to determine the contribution of neuropeptide Y to edema occurrence in neurogenic and hydrostatic pulmonary edema. To induce neurogenic pulmonary edema, fibrinogen and thrombin were injected into the cisterna magna; and to evoke hydrostatic pulmonary edema, saline was infused intravenously. Concentrations of neuropeptide Y in serum and edema fluid were measured using enzyme-linked immunosorbent assay (ELISA), which showed a mean value of 158 nM (95% confidence limit 124-202 nM) in the neurogenic edema fluid, significantly higher than that in the hydrostatic one. Using immunohistochemistry, fluorescent signals reactive to neuropeptide Y were found in the alveolar macrophages and edema fluid in case of fibrin-induced pulmonary edema, but were almost absent in hydrostatic edema and absent in normal lungs. Mean ratio of protein concentrations in edema fluid to that in serum was 74.9 +/- 0.9% in fibrin-induced pulmonary edema, being higher than that in hydrostatic one. From these results, we conclude that neuropeptide Y has a relationship to the high protein concentration ratio, i.e., to increased pulmonary vascular permeability, and consequently may contribute to the development of neurogenic pulmonary edema in rats.
Hamdy
O
O
Department of Anesthesiology, Nagoya University School of Medicine, Japan.
Nishiwaki
K
K
Yajima
M
M
Murakami
H O
HO
Maekawa
H
H
Moy
R T
RT
Shimada
Y
Y
Hotta
Y
Y
Ishikawa
N
N
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D005122:Exudates and Transudates / Q000737:chemistry*; D005340:Fibrinogen / Q000494:pharmacology; D005730:Ganglionic Blockers / Q000494:pharmacology; D018738:Hexamethonium / Q000494:pharmacology; D006874:Hydrostatic Pressure; D007150:Immunohistochemistry; D016676:Macrophages, Alveolar / Q000737:chemistry; D009478:Neuropeptide Y / Q000097:blood / Q000378:metabolism*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D011654:Pulmonary Edema / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D012965:Sodium Chloride; D013917:Thrombin / Q000494:pharmacology
D000959:Antihypertensive Agents; D005730:Ganglionic Blockers; D009478:Neuropeptide Y; D018733:Nicotinic Antagonists; D011506:Proteins; D018738:Hexamethonium; D012965:Sodium Chloride; D005340:Fibrinogen; D013917:Thrombin