Long-term replication of Epstein-Barr virus-derived episomal vectors in the rodent cells.
Plasmids containing the origin of replication, oriP, of the Epstein-Barr virus (EBV) and EBV nuclear antigen-1 genes replicate extrachromosomally in primate cells. However, these plasmids have been believed not to replicate in rodent cells. We demonstrate here that these plasmids can replicate in some types of rodent cells over a long period. This result should offer not only the new insight into the mechanisms of species-specific replication of EBV, but also the possibility that an EBV-based vector can be used for gene transfer experiments in non-primate cells and an animal experiment regarding human gene therapy.
Mizuguchi
H
H
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, Japan. mizuguch@nihs.go.jp
Hosono
T
T
Hayakawa
T
T
D016475:3T3 Cells; D000818:Animals; D015139:Blotting, Southern; D016466:CHO Cells; D002460:Cell Line; D002934:Cinnamates; D006224:Cricetinae; D004351:Drug Resistance; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000502:physiology; D006801:Humans; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008156:Luciferases / Q000235:genetics; D051379:Mice; D010957:Plasmids; D051381:Rats; D018741:Replication Origin; D013997:Time Factors; D014162:Transfection; D014779:Virus Replication
D002934:Cinnamates; D019309:Epstein-Barr Virus Nuclear Antigens; D006921:Hygromycin B; C026273:hygromycin A; D008156:Luciferases