The G-protein FlhF has a role in polar flagellar placement and general stress response induction in Pseudomonas putida.
The flhF gene of Pseudomonas putida, which encodes a GTP-binding protein, is part of the flagellar-motility-chemotaxis operon. Its disruption leads to a random flagellar arrangement in the mutant (MK107) and loss of directional motility in contrast to the wild type, which has polar flagella. The return of a normal flhF allele restores polar flagella and normal motility to MK107; its overexpression triples the flagellar number but does not restore directional motility. As FlhF is homologous to the receptor protein of the signal recognition particle (SRP) pathway of membrane protein translocation, this pathway may have a role in polar flagellar placement in P. putida. MK107 is also compromised in the development of the starvation-induced general stress resistance (SGSR) and effective synthesis of several starvation and exponential phase proteins. While somewhat increased protein secretion in MK107 may contribute to its SGSR impairment, the altered protein synthesis pattern also appears to have a role.
Pandza
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Department of Microbiology and Immunology, Sherman Fairchild Science Building, 299 Campus Drive, Stanford University, Stanford, CA 94305, USA.
Baetens
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Park
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CH
Au
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Keyhan
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Matin
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D000483:Alleles; D001426:Bacterial Proteins / Q000378:metabolism; D004251:DNA Transposable Elements; D005407:Flagella / Q000502:physiology*; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D009154:Mutation; D009876:Operon; D010455:Peptides / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016958:Pseudomonas putida / Q000235:genetics / Q000502:physiology*; D012808:Sigma Factor / Q000378:metabolism
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D010455:Peptides; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; C078317:flhF protein, Bacteria; D020559:Monomeric GTP-Binding Proteins