A functional model for whole limb transplantation in the rat.
To develop a functional model for the study of whole limb transplantation, inbred Lewis rats were used as both donors and recipients. In this model, the recipient biceps femoris muscle was elevated from its distal attachment to preserve part of the adductor function of the limb after surgery. The tibial, peroneal, and sural branches of the sciatic nerve were anastomosed separately to provide faster and more precise functional recovery. For control sensory evaluation, the saphenous branches of the femoral nerve were not reattached. A flat intramedullary pin stabilized with methyl methacrylate was used to rigidly immobilize the femur. The transplanted limbs started bearing weight at 17 to 22 days. Walking on the plantar surface of the hock and adduction of the toes gradually decreased, and the rats developed a normal walking pattern. Sciatic and tibial function indexes, based on walking track analysis, correlated well with clinical observations. In this study, a new model for limb transplantation was developed that provided good and reliable sensory and ambulatory recovery.
Yeh
L S
LS
Department of Surgical and Radiological Sciences at the School of Veterinary Medicine, University of California, Davis, USA. lsyeh@ccms.ntu.edu.tw
Gregory
C R
CR
Theriault
B R
BR
Hou
S M
SM
Lecouter
R A
RA
D000818:Animals; D006614:Hindlimb / Q000294:innervation / Q000637:transplantation*; D008124:Locomotion / Q000502:physiology*; D008297:Male; D008866:Microsurgery; D017288:Pain Threshold / Q000502:physiology; D010525:Peripheral Nerves / Q000503:physiopathology / Q000637:transplantation; D011183:Postoperative Complications / Q000503:physiopathology*; D051381:Rats; D011917:Rats, Inbred Lew; D011984:Sensory Receptor Cells / Q000503:physiopathology*; D012867:Skin / Q000294:innervation; D014185:Transplantation, Isogeneic; D016474:Weight-Bearing / Q000502:physiology