title: 'DNA snapback' peptides.
abstract: Thermal denaturation studies show that 10-15% of the calf thymus DNA in the heat denatured (Tyr-Gly-Tyr-Gly-Tyr)-DNA complex renatures spontaneously after colling. The double-strandness of this DNA was verified by its resistance to single-strand Neurospora endonuclease and by its elution profile on hydroxypatite columns. The renatured DNA isolated by the latter technique was found to contain 56% GC compared to the 41% GC content of the whole thymus DNA. Alternating tryptophanyl-glycyl and histidyl-glycyl peptides also catalyze the same renaturation. A linear correlation was found between the thermal stabilization afforded to the DNA by the various peptides and their ability to "catalyze" DNA strand renaturation.
authors.forename: R L
authors.initials: RL
authors.lastname: Novak
authors.forename: J
authors.initials: J
authors.lastname: Dohnal
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000187:drug effects*; D006358:Hot Temperature; D066298:In Vitro Techniques; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation / Q000187:drug effects; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D009842:Oligopeptides; D004247:DNA