Interleukin 10 suppresses phagocytic and antihyphal activities of human neutrophils.
We investigated the effects of human interleukin 10 (IL-10) on the antibacterial and antifungal activities of human neutrophils (PMNs) against Staphylococcus aureus and Candida albicans. Incubation of PMNs from healthy volunteers with 20-100 ng/ml of IL-10 at 37 degrees C for 1 h suppressed phagocytosis of serum-opsonized S. aureus (P=0.02) and blastoconidia of C. albicans (P<0.01). In contrast, 2-100 ng/ml of IL-10 had no effect on superoxide anion production upon stimulation with phorbol myristate acetate, N-formylmethionyl leucyl phenylalanine, C. albicans blastoconidia or pseudohyphae; neither did it significantly affect conidiocidal or bactericidal activities of PMNs. However, 20-100 ng/ml of IL-10 significantly decreased PMN-induced damage of C. albicans pseudohyphae (P=0.008). The suppression of phagocytic activity of PMNs against S. aureus and blastoconidia of C. albicans as well as the impairment of PMN-induced hyphal damage may have important implications for understanding the immunosuppressive profile of IL-10 in clinical usage.
Roilides
E
E
3rd Dept of Pediatrics, University of Thessaloniki, Greece.
Katsifa
H
H
Tsaparidou
S
S
Stergiopoulou
T
T
Panteliadis
C
C
Walsh
T J
TJ
D000328:Adult; D002176:Candida albicans / Q000276:immunology* / Q000472:pathogenicity; D002467:Cell Nucleus / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000276:immunology* / Q000494:pharmacology; D016753:Interleukin-10 / Q000276:immunology* / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000276:immunology* / Q000472:pathogenicity; D013481:Superoxides / Q000276:immunology
D007166:Immunosuppressive Agents; D011994:Recombinant Proteins; D013481:Superoxides; D016753:Interleukin-10