Subdivision of Salmonella enterica serovar enteritidis phage types PT14b and PT21 by plasmid profiling.
We have shown that plasmid profiling is a sensitive method for further identification of strains of Salmonella enterica serovar enteritidis (S. enteritidis) phage type PT21 and to a lesser extent the strains of phage type PT14b. Five and three plasmid types were identified within 33 strains of phage type PT21 and 19 strains of phage type PT14b, respectively. Plasmid types in strains of phage type PT21 showed significant correlation with geographical origin of the strain. In strains of phage type PT14b a single isolate predominated suggesting that the plasmid designated as 'C' can be directly linked with S. enteritidis PT14b strains. Application of IS200 fingerprinting did not reveal any other differences and showed just one copy of IS200 in all the 52 analysed strains. All the strains were tested for antibiotic resistance and only four strains were resistant to ampicillin, cefotaxime, cefuroxime and cotrimoxazole. This indicates that low molecular weight plasmids in Salmonella enteritidis are not responsible for the spread of antibiotic resistance.
Rychlik
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Veterinary Research Institute, Hudcova 70, 621 32, Brno, Czech Republic. rychlik@vri.cz
Svestkova
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Karpiskova
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D000818:Animals; D016172:DNA Fingerprinting; D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005060:Europe; D006801:Humans; D007091:Image Processing, Computer-Assisted; D010957:Plasmids / Q000737:chemistry / Q000145:classification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012480:Salmonella Infections / Q000175:diagnosis*; D012483:Salmonella Phages / Q000737:chemistry / Q000145:classification*; D012477:Salmonella enteritidis / Q000737:chemistry / Q000145:classification*
D015342:DNA Probes; D004279:DNA, Viral; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases