title: Correlation between three-dimensional structure and chemical reactivity of transfer RNA.
abstract: The bases of yeast tRNA(Phe) which react with carbodiimide and methoxyamine have been determined and this information has been combined with chemical modification studies of other workers to produce a composite picture of base accessibility in this tRNA. The results are compared with the three-dimensional structure which we have recently determined. The bases which react chemically lie in exposed positions in the three-dimensional model and those which do not are either in the double helical stem regions or else are involved in maintaining the tertiary structure through pairing or stacking interactions.
authors.forename: J D
authors.initials: JD
authors.lastname: Robertus
authors.forename: J E
authors.initials: JE
authors.lastname: Ladner
authors.forename: J T
authors.initials: JT
authors.lastname: Finch
authors.forename: D
authors.initials: D
authors.lastname: Rhodes
authors.forename: R S
authors.initials: RS
authors.lastname: Brown
authors.forename: B F
authors.initials: BF
authors.lastname: Clark
authors.forename: A
authors.initials: A
authors.lastname: Klug
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002234:Carbodiimides; D006898:Hydroxylamines; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012360:RNA, Transfer, Phe / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D002234:Carbodiimides; D006898:Hydroxylamines; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe; C005214:methoxyamine