Adherence of Fusobacterium necrophorum subsp. necrophorum to ruminal cells derived from bovine rumenitis.
Fusobacterium necrophorum subsp. necrophorum strain VPI 2891 was shown to adhere to the surfaces of ruminal cells derived from bovine rumenitis. The strain also attached to bovine type 1 collagen. Treatment of the bacterium with antiserum to bacterial cells reduced attachment. The bacterial attachment was also markedly reduced when the ruminal cells had been pretreated with anticollagen serum. Fluorescence specific for the collagen was demonstrated on the surface of bovine tissue affected with rumenitis. These findings suggest that F. necrophorum subsp. necrophorum strain VPI 2891 adheres to the ruminal cells derived from rumenitis tissue and that the attachment may be mediated by cellular collagen.
Takayama
Y
Y
Laboratory of Animal Health, Tokyo University of Agriculture, Tokyo and Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yamaguchi City, Japan.
Kanoe
M
M
Maeda
K
K
Okada
Y
Y
Kai
K
K
D000818:Animals; D001422:Bacterial Adhesion; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003094:Collagen / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005674:Fusobacterium Infections / Q000382:microbiology / Q000662:veterinary*; D005675:Fusobacterium necrophorum / Q000502:physiology*; D012417:Rumen / Q000166:cytology / Q000382:microbiology*; D013272:Stomach Diseases / Q000382:microbiology / Q000662:veterinary*
D003094:Collagen