Deoxyribonucleic acid of Cancer pagurus. II. Template activity for a DNA-dependent DNA polymerase of eukaryotic cells.
The template activity of Cancer pagurus DNA and its two components (poly d(A-T) and main component) in response to a DNA polymerase purified from regenerating rat liver has been studied and compared to the results previously obtained with synthetic templates. In the double-stranded native state, whole crab DNA and the main component were poor templates. Their replication was increased by thermal denaturation and inhibited by actinomycin. Like the synthetic copolymer poly[d(A-T).d(T-A)], native crab poly d(A-T) could be copied and its duplication was not inhibited by actinomycin. The structural difference between native poly d(A-T) Form I, isolated on a density gradient, and partially renatured poly d(A-T) Form II, isolated on hydroxylapatite, resulted in a modification of their template activity. The kinetic studies of [(3)H] dGMP and [(3)H] dAMP incorporation confirmed the importance of single-stranded regions (particulary dC regions) in the initiation of the in vitro duplication.
de Recondo
A M
AM
Londos-Gagliardi
D
D
Aubel-Sadron
G
G
D000818:Animals; D003386:Brachyura / Q000737:chemistry*; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004277:DNA, Single-Stranded / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004706:Endodeoxyribonucleases / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008115:Liver Regeneration; D009492:Neurospora crassa / Q000201:enzymology; D009691:Nucleic Acid Denaturation; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011067:Poly dA-dT / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013698:Templates, Genetic
D004277:DNA, Single-Stranded; D005656:Fungal Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D003609:Dactinomycin; D011067:Poly dA-dT; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases