Reverse correlation between urine nitric oxide metabolites and insulin resistance in patients with type 2 diabetes mellitus.
We studied the possible relationship between nitric oxide (NO) production and insulin resistance in patients with type 2 diabetes mellitus. Urine NO metabolites (NOx) were measured as an index for NO production by HPLC combined with a Cd column, Griess reaction and a spectrophotometer in 403 healthy control subjects and 102 hospitalized patients with type 2 diabetes. Glucose infusion rate (GIR) was measured as a reverse index for insulin resistance by euglycemic glucose clamp study using an artificial pancreas in 20 of 102 diabetic patients. Urine NOx was lower in the patients with type 2 diabetes than in healthy control subjects (mean+/-SE: 3.18 +/-0.02 versus 3.25 +/-0.01 log[-micromol/gCr], p<0.01). Urine NOx was correlated with body mass index (BMI) in 102 diabetic patients (r= -0.372, p<0.001), but not related to either age, sex, fasting plasma glucose, HbA1c or blood pressure. Urine NOx was correlated with GIR independent of BMI in 20 diabetic patients (r=0.774, P<0.0001). These findings suggest that NO production is closely related with insulin resistance in patients with type 2 diabetes.
Kurioka
S
S
Department of Medicine, Shimane Medical University, Izumo, Japan.
Koshimura
K
K
Murakami
Y
Y
Nishiki
M
M
Kato
Y
Y
D000328:Adult; D000368:Aged; D015992:Body Mass Index; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy / Q000652:urine; D005260:Female; D005947:Glucose / Q000494:pharmacology; D015309:Glucose Clamp Technique; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000652:urine*; D009573:Nitrites / Q000652:urine*; D019397:Pancreas, Artificial; D012016:Reference Values
D009566:Nitrates; D009573:Nitrites; D005947:Glucose