Modulation of G-protein linked cAMP accumulation in immortalized murine cortical astrocytes by retroviral infection.
The present study characterized beta-adrenergic receptors (beta-AR) in a clonal cell line (C1) immortalized from cerebral cortical astroglial cells of FVB/N mice. We also determined whether the wild type Moloney murine leukemia virus (wt-MoMuLV) and one of its neuropathogenic mutants, ts1-MoMuLV, modulated the beta-AR system in these cells. We observed that C1 cells possess a functional beta-AR system coupled to cAMP accumulation and capable of normal agonist-induced regulation (desensitization). Significant increases were observed in forskolin stimulated cAMP accumulation in C1 cells infected by wt MoMuLV and by ts1-MoMuLV. In contrast, the cAMP response to beta-AR stimulated by isoproterenol was relatively spared after viral exposure.
Raofi
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S
Division of Pharmacology and Toxicology, College of Pharmacy, University of Texas at Austin, Austin, TX, USA.
Wong
P K
PK
Wilcox
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RE
D000262:Adenylyl Cyclases / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002461:Cell Line, Transformed / Q000737:chemistry / Q000201:enzymology / Q000821:virology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D008979:Moloney murine leukemia virus; D019579:Neocortex / Q000166:cytology; D011433:Propranolol / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D012192:Retroviridae Infections / Q000378:metabolism*; D014412:Tumor Virus Infections / Q000378:metabolism*
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D011433:Propranolol; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases; D007545:Isoproterenol