title: Activities of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori.
abstract: Chronic Helicobacter pylori disease is reduced with Allium vegetable intake. This study was designed to assess the in vivo anti-H. pylori potential of a variety of garlic substances. The garlic materials all showed substantial but widely differing anti-H. pylori effects against all strains and isolates tested. The MICs (range, 8 to 32 microg/ml) and minimum bactericidal concentrations (MBCs) (range, 16 to 32 microg/ml) of undiluted garlic oil (GO) were smaller than those of garlic powder (GP) (MIC range, 250 to 500 microg/ml; MBC range, 250 to 500 microg/ml) but greater than the MIC of allicin (4. 0 microg/ml) (Table 2) present in GP. Allicin (MIC, 6 microg/ml; MBC, 6 microg/ml) was more potent than diallyl disulfide (MIC range, 100 to 200 microg/ml; MBC range, 100 to 200 microg/ml), its corresponding sulfide, but of a strength similar to that of diallyl tetrasulfide (MIC range, 3 to 6 microg/ml; MBC range, 3 to 6 microg/ml). Antimicrobial activity of the diallyl sulfides increased with the number of sulfur atoms. Time course viability studies and microscopy showed dose-dependent anti-H. pylori effects with undiluted GO, GP, allicin, and diallyl trisulfide after a lag phase of ca. 1 to 2 h. Substantial in vitro anti-H. pylori effects of pure GO and GP and their diallyl sulfur components exist, suggesting their potential for in vivo clinical use against H. pylori infections.
authors.affiliation: School of Health Sciences, University of Wolverhampton, Wolverhampton WV1 1DJ, United Kingdom.
authors.forename: E A
authors.initials: EA
authors.lastname: O'Gara
authors.forename: D J
authors.initials: DJ
authors.lastname: Hill
authors.forename: D J
authors.initials: DJ
authors.lastname: Maslin
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D004220:Disulfides / Q000494:pharmacology; D005737:Garlic; D016480:Helicobacter pylori / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D010946:Plants, Medicinal; D013440:Sulfides / Q000494:pharmacology*; D013441:Sulfinic Acids / Q000494:pharmacology*
chemical_list: D000498:Allyl Compounds; D004220:Disulfides; D010936:Plant Extracts; D010938:Plant Oils; D013440:Sulfides; D013441:Sulfinic Acids; C006452:allicin; C028009:diallyl disulfide