Production of sexed lambs after biopsy of ovine blastocyts produced in vitro.
Embryos were generated by in vitro fertilization of in vitro-matured oocytes, cultured to the blastocyst stage, biopsied for sex determination by a PCR-based procedure, and transferred to synchronized recipients. Three out of 5 sheep (60%) were diagnosed pregnant, and 4 lambs of predicted sex were born.
Kochhar
H S
HS
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph.
Buckrell
B C
BC
Pollard
J W
JW
King
W A
WA
D000818:Animals; D001755:Blastocyst; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011256:Pregnancy Outcome; D019849:Sex Determination Processes; D012756:Sheep / Q000502:physiology*