Differential effect of clofibrate on acetyl-CoA carboxylase mRNA level in rat white and brown adipose tissue.
Regulation of some lipogenic enzyme gene expression by clofibrate was studied in rat white and brown adipose tissue. In white adipose tissue the drug administration for 14 days to rats resulted in the increase in acetyl-CoA carboxylase, ATP-citrate lyase, and glucose 6-phosphate dehydrogenase mRNA levels. Opposing effect of clofibrate on the acetyl-CoA carboxylase, ATP-citrate lyase, and glucose 6-phosphate dehydrogenase mRNA levels was found in brown adipose tissue. These data indicate a tissue specificity of clofibrate action on lipogenic enzyme gene expression. The results presented in this paper provide further evidence that hypolipidaemia caused by the treatment with clofibrate cannot be related to the inhibition of fatty acid synthesis in white adipose tissue in rat.
Karbowska
J
J
Department of Biochemistry, Medical University of Gdansk, Poland.
Kochan
Z
Z
Swierczyński
J
J
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D000273:Adipose Tissue / Q000187:drug effects* / Q000201:enzymology; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D002994:Clofibrate / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology
D000924:Anticholesteremic Agents; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D005954:Glucosephosphate Dehydrogenase; D000103:Acetyl-CoA Carboxylase; D002994:Clofibrate