Does aluminum inhibit pollen germination via extracellular calmodulin?
The effect of aluminum (Al) on pollen germination and its mechanism of action were investigated. Pollen germination and pollen tube elongation were inhibited by Al at pH 4.5. This inhibitory effect was reversed by the addition of purified calmodulin (CaM), whereas neither the calcium binding-protein S-100 nor Al chelator citric acid at the same concentrations had any obvious effect on Al-inhibited pollen germination. The presence of either the membrane-impermeable CaM inhibitor anti-CaM antiserum or Ca2+ chelator EGTA completely suppressed the effect of exogenous CaM. These results indicate the involvement of extracellular calmodulin in the short-term effects of Al on pollen germination and pollen tube elongation.
Ma
L G
LG
Institute of Molecular Cell Biology, Hebei Normal University, P R China.
Fan
Q S
QS
Yu
Z Q
ZQ
Zhou
H L
HL
Zhang
F S
FS
Sun
D Y
DY
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D002147:Calmodulin / Q000276:immunology / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism* / Q000633:toxicity; D019343:Citric Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D011058:Pollen / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011817:Rabbits; D009418:S100 Proteins / Q000494:pharmacology
D017607:Aluminum Compounds; D002147:Calmodulin; D002712:Chlorides; D007106:Immune Sera; D009418:S100 Proteins; D019343:Citric Acid; D000077410:Aluminum Chloride; D004533:Egtazic Acid