title: Serological analysis of a cryptosporidiosis epidemic.
abstract: BACKGROUND
  A cryptosporidiosis epidemic occurred among residents and visitors to Collingwood, Ontario, during March 1996. Fifty-five per cent of 36 confirmed cases were Collingwood visitors and 57% of Collingwood resident cases were under 10 years of age. The low level of reported diarrhoeal illness among adult Collingwood residents caused government officials and physicians to question whether an epidemic had occurred in Collingwood.
  
  
  METHODS
  To better evaluate the extent of the epidemic, anonymous surplus sera from 89 adult Collingwood residents, collected for routine tests prior to, during and after the epidemic, and from 80 adult Toronto residents were tested using a Western blot assay for IgG antibody response to two Cryptosporidium antigen groups (15/17-kDa and 27-kDa).
  
  
  RESULTS
  For sera collected from 1 January 1996 to 17 June 1996, a higher fraction of Collingwood residents had a detectable serological response (P < 0.002) and the mean intensity of serological responses was higher for Collingwood than Toronto residents (P < 0.001). The mean intensity of serological responses for Collingwood residents was higher in specimens drawn during the 8 weeks following the initial case reports compared to those drawn before or after this period (15/17-kDa, P < 0.02; 27-kDa, P < 0.10).
  
  
  CONCLUSIONS
  These elevated serological responses indicate that Cryptosporidium infections among Collingwood residents likely occurred more commonly than illness reports suggested, consistent with a community-wide cryptosporidiosis epidemic. Similar studies should be considered in future suspected cryptosporidiosis epidemic investigations.
authors.affiliation: The Southwest Center for Managed Care Research, Lovelace Respiratory Research Institute, Albuquerque, NM 98108, USA.
authors.forename: F J
authors.initials: FJ
authors.lastname: Frost
authors.forename: T
authors.initials: T
authors.lastname: Muller
authors.forename: G F
authors.initials: GF
authors.lastname: Craun
authors.forename: D
authors.initials: D
authors.lastname: Fraser
authors.forename: D
authors.initials: D
authors.lastname: Thompson
authors.forename: R
authors.initials: R
authors.lastname: Notenboom
authors.forename: R L
authors.initials: RL
authors.lastname: Calderon
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D002648:Child; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology / Q000635:transmission; D003458:Cryptosporidium / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D009864:Ontario / Q000453:epidemiology; D012189:Retrospective Studies; D016036:Seroepidemiologic Studies
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G