Antioxidant properties of pyrrolidine dithiocarbamate and its protection against Cr(VI)-induced DNA strand breakage.
Pyrrolidine dithiocarbamate (PDTC) is considered an antioxidant and is frequently used to study the role of free radical reactions in various biological processes and against free radical-induced cellular injuries. However, its antioxidant properties are not characterized. In this study, electron spin resonance (ESR) was used to investigate the antioxidant potential of PDTC with hydroxyl radical (*OH) and superoxide anion radicals (O2*-). The Fenton reaction [Fe(II) + H2O2 --> Fe(II) + *OH + OH-)] and xanthine and xanthine oxidase were used as sources of *OH and O2*- radicals, respectively. The results show that PDTC effectively scavenges *OH radicals with a reaction rate constant of approximately 2.73 x 10(10) M(-1) s(-1), which is comparable to other efficient *OH radical scavengers, such as ascorbate and glutathione. PDTC is also able to scavenge O2*- radicals. Through its antioxidant properties, PDTC protects against Cr(VI)-induced DNA strand breakage.
Shi
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Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia, USA. xas0@cdc.gov
Leonard
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Wang
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Ding
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D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002857:Chromium / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D011759:Pyrrolidines / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D013481:Superoxides / Q000737:chemistry / Q000378:metabolism; D013859:Thiocarbamates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology
D000975:Antioxidants; D005609:Free Radicals; D011759:Pyrrolidines; D013859:Thiocarbamates; D002857:Chromium; D013481:Superoxides; C020972:pyrrolidine dithiocarbamic acid; D017665:Hydroxyl Radical