title: Heterologous expression of an immunogenic pneumococcal type 3 capsular polysaccharide in Lactococcus lactis.
abstract: In order to develop a new system for the analysis of capsular biosynthetic pathways we have explored the possibility of expressing type 3 capsular polysaccharide (CPS) from the pathogen Streptococcus pneumoniae in Lactococcus lactis, an unencapsulated lactic acid bacterium being developed as a vaccine delivery vehicle for mucosal immunization. Only three of the four type 3 CPS biosynthesis genes were found to be necessary for the abundant formation (120 mg liter(-1)) of an extracellular type 3 CPS in L. lactis, implying a role for the type 3-specific synthase in the extracellular transport of the CPS or implying the existence of an alternative export system in L. lactis. The authenticity of the expressed heterologous polysaccharide was established by chemical and immunological analyses. Proton and carbon nuclear magnetic resonance spectroscopy of CPSs purified from L. lactis and S. pneumoniae showed that the two CPS structures were identical. When mice were immunized intraperitoneally with 3.5 x 10(6) CFU of live recombinant lactococci expressing a total of approximately 0.5 microgram of type 3 CPS, the immune responses elicited appeared identical to those observed in mice inoculated with 0.5 microgram of type 3 CPS purified from S. pneumoniae. These findings show that L. lactis is a useful host in which to study the role and function of genes involved in the production of bacterial capsules. Additionally, L. lactis shows potential as a host for the safe production of capsule antigens and as a vaccine delivery vehicle for polysaccharide antigens.
authors.affiliation: Cortecs Centre for Vaccine Discovery, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom. gilbert@biomserv.univ-lyon1.fr
authors.forename: C
authors.initials: C
authors.lastname: Gilbert
authors.forename: K
authors.initials: K
authors.lastname: Robinson
authors.forename: R W
authors.initials: RW
authors.lastname: Le Page
authors.forename: J M
authors.initials: JM
authors.lastname: Wells
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D001428:Bacterial Vaccines / Q000276:immunology*; D003001:Cloning, Molecular; D015870:Gene Expression; D007274:Injections, Intraperitoneal; D013294:Lactococcus lactis / Q000235:genetics / Q000276:immunology*; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D011135:Polysaccharides, Bacterial; D014614:Vaccines, Synthetic; C026604:pneumococcal polysaccharide, type III