Cisplatin DNA adduct detection and depurination measured by 32P DNA radiolabeling and two-dimensional thin-layer chromatography: a time and concentration study.
Platinum-based chemotherapies cause the formation of DNA adducts and have profound effects on DNA. This study measured cis-diamminedichloroplatinum II (cisplatin) DNA adducts by 32P-radiolabeling DNA, enzymatically digesting radiolabeled DNA, separating the formed adducts on two-dimensional thin-layer chromatography, and quantitating the adducts with autoradiography and densitometry. HeLa DNA was incubated with cisplatin at varying concentrations (6.25-325 nM) and times (0 min to 72 hr). Cisplatin rapidly depurinated dGMP and dAMP (90%, 15-min incubation with 325 nM cisplatin). Partial depurination of dGMP (15%) and dAMP (25%) occurred with lower cisplatin concentrations at equal incubation times. A minimum of four new adducts, with relatively rapid migratory patterns, were detected at high cisplatin concentrations with short incubation times. These results indicate that the depurination of DNA correlates with DNA adduct formation and that the quantification of these adducts may be applicable to monitoring tumor and host cell response to cisplatin chemotherapy.
Farah
N
N
Department of Pathology, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, New York, USA.
Dresner
H S
HS
Searles
K J
KJ
Winiarsky
R
R
Moosikasuwan
M
M
Cajigas
A
A
Hahm
S
S
Steinberg
J J
JJ
D000249:Adenosine Monophosphate / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D002945:Cisplatin / Q000494:pharmacology*; D018736:DNA Adducts / Q000032:analysis*; D004273:DNA, Neoplasm / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006157:Guanosine Monophosphate / Q000378:metabolism*; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D010761:Phosphorus Radioisotopes
D000970:Antineoplastic Agents; D018736:DNA Adducts; D004273:DNA, Neoplasm; D010761:Phosphorus Radioisotopes; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D002945:Cisplatin