In vitro susceptibility tests for cationic peptides: comparison of broth microdilution methods for bacteria that grow aerobically.
The in vitro susceptibilities of 90 clinical isolates of gram-positive and gram-negative aerobic bacteria to six cationic peptides, buforin II, cecropin P1, indolicidin, magainin II, nisin, and ranalexin, were evaluated by two broth microdilution methods. The first method was performed according to the procedures outlined by the National Committee for Clinical Laboratory Standards for bacteria that grow aerobically, while the second was performed according to the procedures recently proposed by the R. E. W. Hancock laboratory for testing antimicrobial peptides. Overall, the first method produced MICs two- and fourfold higher than the second method.
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Institute of Infectious Diseases and Public Health, University of Ancona, Italy. cmalinf@popcsi.unian.it
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D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D054806:Magainins; D009561:Nisin / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology; D029867:Xenopus Proteins
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; D010455:Peptides; D010456:Peptides, Cyclic; D011506:Proteins; D029867:Xenopus Proteins; C097849:buforin II; C073143:indolicidin; C053301:magainin 2 peptide, Xenopus; D009561:Nisin; C086618:ranalexin; C070016:cecropin P1-LI