The 19-kD antigen and protective immunity in a murine model of tuberculosis.
The 19-kD antigen is a cell wall-associated lipoprotein present in Mycobacterium tuberculosis and in bacille Calmette-Guérin (BCG) vaccine strains. Expression of the 19-kD antigen as a recombinant protein in two saprophytic mycobacteria-M. vaccae and M. smegmatis-resulted in abrogation of their ability to confer protection against M. tuberculosis in a murine challenge model, and in their ability to prime a DTH response to cross-reactive mycobacterial antigens. Induction of an immune response to the 19-kD antigen by an alternative approach of DNA vaccination had no effect on subsequent M. tuberculosis challenge. These results are consistent with a model in which the presence of the 19-kD protein has a detrimental effect on the efficacy of vaccination with live mycobacteria. Targeted inactivation of genes encoding selected antigens represents a potential route towards development of improved vaccine candidates.
Yeremeev
V V
VV
Laboratory for Immunogenetics, Central Institute for Tuberculosis, Moscow, Russia.
Lyadova
I V
IV
Nikonenko
B V
BV
Apt
A S
AS
Abou-Zeid
C
C
Inwald
J
J
Young
D B
DB
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001500:BCG Vaccine / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D051379:Mice; D008810:Mice, Inbred C57BL; D009161:Mycobacterium / Q000276:immunology; D020102:Mycobacterium smegmatis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology / Q000503:physiopathology / Q000517:prevention & control*; D014611:Vaccination; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
C087864:19 kDa antigen, Mycobacterium; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA