Inactivation of the glutamate decarboxylase gene in Lactococcus lactis subsp. cremoris.
Lactococcus lactis subsp. lactis strains show glutamate decarboxylase activity, whereas L. lactis subsp. cremoris strains do not. The gadB gene encoding glutamate decarboxylase was detected in the L. lactis subsp. cremoris genome but was poorly expressed. Sequence analysis showed that the gene is inactivated by the frameshift mutation and encoded in a nonfunctional protein.
Nomura
M
M
Department of Animal Products, National Institute of Animal Industry, Norindanchi, Tsukuba, Ibaraki 305-0901, Japan. nomura@niai.affrc.go.jp
Kobayashi
M
M
Ohmomo
S
S
Okamoto
T
T
D001483:Base Sequence; D016368:Frameshift Mutation; D005968:Glutamate Decarboxylase / Q000235:genetics*; D013294:Lactococcus lactis / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D005968:Glutamate Decarboxylase