Cross-reactivity of anti-Taenia crassiceps cysticerci immune antibodies with Taenia solium antigens.
A comparative study of antibody production was carried out using BALB/c mice immunized with 20 or 50microg vesicular fluid (VF)-Tcra (Taenia crassiceps) antigens, and gel of <30kD or eluate from <30kD peptides. Good IgM, IgA and IgG levels were detected by ELISA-Tcra and the antibodies presented reactivity with the <20kD peptides when tested by immunoblotting-Tcra. The antibodies from animals immunized with 20 and 50microg presented high anti-Tso cross-reactivity in ELISA (IgG>>IgM and IgA). All groups presented IgG antibodies identifying the 12kD Tso-peptide.
Espíndola
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NM
Faculty of Pharmaceutical Sciences, University of São Paulo, Av Prof. Lineu Prestes 580, Bloco 17, 05508-900, São Paulo, Brazil.
De Gaspari
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EN
Nakamura
P M
PM
Vaz
A J
AJ
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007115:Immunization Schedule; D051379:Mice; D008807:Mice, Inbred BALB C; D020019:Neurocysticercosis / Q000276:immunology*; D013621:Taenia / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth