title: Rad24 is essential for proliferation of diploid cells in fission yeast.
abstract: The rad24(+) gene of Schizosaccharomyces pombe encodes a ubiquitously expressed 14-3-3 protein. We report here that Deltarad24 cells displayed a defect in diploid colony formation, although they conjugated efficiently. We found that a cumulative deletion of mei2(+) gene almost completely suppressed this defect, and demonstrated using two-hybrid analysis that Rad24 protein directly associates with Mei2 protein by recognizing Ser-438 which is a phosphorylation target of Pat1 kinase. We conclude that constitutive progression to meiosis, caused by lack of Mei2 inhibition due to the absence of Rad24 protein, is the primary cause of the proliferative deficiency observed in Deltarad24 cells.
authors.affiliation: Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: D
authors.initials: D
authors.lastname: Okuzaki
authors.forename: N
authors.initials: N
authors.lastname: Yabuta
authors.forename: T
authors.initials: T
authors.lastname: Yoneki
authors.forename: H
authors.initials: H
authors.lastname: Nojima
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D004265:DNA Helicases / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D047908:Intracellular Signaling Peptides and Proteins; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C088896:mei2 protein, S pombe; C501114:rad24 protein, S pombe; C489574:rad25 protein, S pombe; C071596:ste11 protein, S pombe; D004265:DNA Helicases