Boron nutrition of cultured tobacco BY-2 cells. III. Characterization of the boron-rhamnogalacturonan II complex in cells acclimated to low levels of boron.
Cultured cells of tobacco (Nicotiana tabacum L.) BY-2 which could propagate at the same rate as the parent cells (1 mg B liter(-1)) under a lower level of boron (0.01 mg B liter(-1)) were obtained. The selected cells had swollen cell walls. In the parent cells, all the RG-II occurred as a B-RG-II complex, however, two-thirds of the RG-II occurred in a monomeric form in the selected cells.
Matoh
T
T
Division of Applied Life Sciences, Faculty of Agriculture, Kyoto University, Japan. matoh@kais.kyoto-u.ac.jp
Takasaki
M
M
Kobayashi
M
M
Takabe
K
K
D001895:Boron / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D002478:Cells, Cultured; D007150:Immunohistochemistry; D010368:Pectins / Q000378:metabolism*; D010947:Plants, Toxic; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
C042492:rhamnogalacturonan II; D010368:Pectins; D001895:Boron