Possible postsynaptic action of aminoglycosides in the frog rectus abdominis.
The present study was undertaken to investigate the postsynaptic effects of aminoglycosides on contractions evoked by acetylcholine (ACh), KCl, electrical field stimulation (EFS) and Na(+)- and Ca(2+)-free Ringer solution with 0.2 mM Na2 EDTA (NaFCaFR) in the isolated frog rectus abdominis. Neomycin inhibited contraction elicited by ACh, NaFCaFR, and EFS at the higher frequencies (8 and 10 Hz) but not those elicited by KCl and EFS at the lower frequencies (2, 3 and 5 Hz). D-tubocurarine inhibited ACh-induced contractions in a concentration-dependent manner. In addition, drug reduced EFS-evoked contractions to a limited extent. Lower concentrations (10(-5), 5 x 10(-5), 10(-4), 2 x 10(-4) and 3 x 10(-4) M) but not higher concentrations (4 x 10(-4) and 5 x 10(-4) M) of methoxyverapamil exhibited a concentration-dependent inhibitory action on NaFCaFR-induced contractions. Similar inhibitions of the same type of contraction were displayed by aminoglycosides (neomycin, streptomycin, netilmycin, gentamycin and amikacin). These results suggest that in addition to their antagonistic action on nicotinic receptors in the frog rectus abdominis, aminoglycosides may exert stabilizing effects on some functional components contributing to contractions at the membrane.
Karataş
Y
Y
Department of Pharmacology, Medical Faculty, Cukurova University, Balcali Adana, Turkey.
Ergün
Y
Y
Göçmen
C
C
Seçilmiş
A
A
Singirik
E
E
Dikmen
A
A
Baysal
F
F
D000109:Acetylcholine / Q000494:pharmacology; D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D066298:In Vitro Techniques; D007552:Isotonic Solutions / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009469:Neuromuscular Junction / Q000187:drug effects / Q000502:physiology*; D003473:Neuromuscular Nondepolarizing Agents / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011894:Rana pipiens; D017568:Rectus Abdominis / Q000187:drug effects / Q000502:physiology*; D000077331:Ringer's Solution; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D014403:Tubocurarine / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D007552:Isotonic Solutions; D003473:Neuromuscular Nondepolarizing Agents; D014665:Vasodilator Agents; D011189:Potassium Chloride; D000077331:Ringer's Solution; D004492:Edetic Acid; D000109:Acetylcholine; D014403:Tubocurarine