TCR-Vbeta repertoire analysis with RT-PCR was useful for the early detection of pulmonary relapsed T-cell lymphoma after autologous peripheral blood stem cell transplantation.
Pulmonary recurrence of malignant lymphoma is a rare event after stem cell transplantation. We report here a 45-year-old male who was successfully diagnosed with relapsed pulmonary T-cell lymphoma using an RT-PCR method. Clonal expansion of T cells expressing identical TCR V-D-J junction size (Vbeta5-Jbeta1.5) was demonstrated in lymphocyte groups obtained from both bronchoalveolar lavage fluid at relapse, and paraffin embedded lymph node samples resected when he was first diagnosed with angioimmunoblastic T-cell lymphoma. This method provided evidence to diagnose relapsed pulmonary angioimmunoblastic T-cell lymphoma in its early phase.
Hase
H
H
The Department of Hematology/Oncology, The University of Tokyo, Japan.
Tani
K
K
Nagayama
H
H
Watari
K
K
Takahashi
S
S
Ooi
J
J
Shirafuji
N
N
Iseki
T
T
Nakazaki
Y
Y
Yamashita
T
T
Nakamura
T
T
Masunaga
A
A
Maekawa
T
T
Tojo
A
A
Asano
S
S
D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000601:surgery; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism; D016399:Lymphoma, T-Cell / Q000175:diagnosis* / Q000378:metabolism* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000378:metabolism / Q000601:surgery; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016693:Receptors, Antigen, T-Cell, alpha-beta