Inhibition by long-chain acyl-CoAs of glucose 6-phosphate metabolism in plastids isolated from developing embryos of oilseed rape (Brassica napus L.).
The effects of long-chain acyl-CoA (lcACoA) esters (both added exogenously and synthesized de novo) and acyl-CoA binding protein (ACBP) on plastidial glucose 6-phosphate (Glc6P) and pyruvate metabolism were examined using isolated plastids. The binding of lcACoA esters by ACBP stimulated the utilization of Glc6P for fatty acid synthesis, starch synthesis and reductant supply via the oxidative pentose phosphate (OPP) pathway. Stimulation occurred at low (1-10 microM) concentrations of ACBP. Pyruvate-dependent fatty acid synthesis was not directly affected by ACBP. However, addition of ACBP did increase the Glc6P-dependent stimulation of pyruvate utilization mediated through the OPP pathway. On the basis of these experiments, we conclude that lcACoA esters may inhibit Glc6P uptake into plastids, and that this inhibition is relieved by ACBP. We also suggest that utilization of other substrates for fatty acid synthesis may be affected by lcACoA/ACBP via their effects on the OPP pathway.
Johnson
P E
PE
John Innes Centre, Norwich Research Park, Colney, Norwich, Norfolk NR4 7UH, U.K. p.johnson@bbsrc.ac.uk
Fox
S R
SR
Hills
M J
MJ
Rawsthorne
S
S
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D001937:Brassica; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli; D019298:Glucose-6-Phosphate / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018087:Plastids / Q000187:drug effects* / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012639:Seeds / Q000378:metabolism
D000214:Acyl Coenzyme A; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D019298:Glucose-6-Phosphate; D019289:Pyruvic Acid