title: Prevalence of Brachyspira species isolated from diarrhoeic pigs in Brazil.
abstract: Pathogenic intestinal spirochaetes of pigs include Brachyspira (formerly Serpulina) hyodysenteriae, the cause of swine dysentery, and Brachyspira pilosicoli, the cause of porcine colonic spirochetosis (PCS). The purpose of this study was to assess the relative importance of Brachyspira species in diarrhoeal disease of growing pigs on farms in southern Brazil. The intensity and pattern of haemolysis, the production of indole and the hydrolysis of hippurate by reference and field porcine intestinal spirochaetes were compared with 16S-ribosomal RNA (mRNA)- and 23S-rRNA-based polymerase chain reaction assays for the identification of B hyodysenteriae and B pilosicoli. Between July and October 1998, 206 rectal swabs were taken from pigs on 17 farms with a history of diarrhoea developing within 30 days after they had been moved from nursery to growing facilities. Of 49 beta-haemolytic spirochaetes that were cultured, 29 (59.2 per cent) were grown in pure culture for phenotypic and genotypic characterisation, leaving 20 untyped. Of the 29 typed isolates, eight isolates obtained from six farms were identified as B hyodysenteriae, and 15 isolates obtained from seven other farms were identified as B pilosicoli; the remaining six isolates were identified as weakly beta-haemolytic commensal spirochaetes. There was complete agreement between the results of the phenotypic and genotypic analyses.
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal de Rio de Janeiro, RJ, Brazil.
authors.forename: D E
authors.initials: DE
authors.lastname: Barcellos
authors.forename: M R
authors.initials: MR
authors.lastname: Mathiesen
authors.forename: M
authors.initials: M
authors.lastname: de Uzeda
authors.forename: I I
authors.initials: II
authors.lastname: Kader
authors.forename: G E
authors.initials: GE
authors.lastname: Duhamel
mesh_terms: D000818:Animals; D017401:Brachyspira hyodysenteriae / Q000235:genetics / Q000302:isolation & purification*; D001938:Brazil / Q000453:epidemiology; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000662:veterinary*; D005838:Genotype; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D012338:RNA, Ribosomal, 23S / Q000302:isolation & purification; D013145:Spirochaetales Infections / Q000453:epidemiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S