title: Effects of date extract on adhesion of Candida species to human buccal epithelial cells in vitro.
abstract: The adherence of three Candida species to human buccal epithelial cells (BEC) following treatment with different concentrations of date extract was investigated in vitro, as well as the effect of a mouth rinse with date extract on the adhesion of yeast to BEC. Adhesion of C. albicans, C. tropicalis and C. kefyr to BEC was significantly reduced after both short- and long-term periods of yeast exposure to various concentrations of date extract (reduction between 25% and 52% of the control value). A similar inhibition of adherence was observed upon pre-incubation of BEC with date extract. There was a significant reduction (P<0.001) in the adherence of yeast to BEC collected immediately or 5-20 min after an oral rinse with 10% date extract. No statistically significant difference was observed in the adhesion of BEC collected 30 min after an oral rinse with date extract and control BEC. In addition, pre-treatment of either Candida or BEC, or both, with date extract resulted in reduced adherence, the magnitude of which was largest when both types of cells were pre-treated. Date extract also inhibited germ-tube formation of C. albicans (56-85% inhibition), which might contribute to the effects on adherence.
authors.affiliation: Department of Biological Sciences, Faculty of Science, The Hashemite University, Zarqa, Jordan.
authors.forename: K H
authors.initials: KH
authors.lastname: Abu-Elteen
mesh_terms: D000268:Adhesiveness; D000328:Adult; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000382:microbiology*; D005638:Fruit; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology*; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts