title: Effects of bradykinin on coronary blood flow and vasomotion in transplant patients.
abstract: OBJECTIVE
  To evaluate the effects of exogenous bradykinin on coronary epicardial and microcirculatory tone in transplant patients (HTXs), and to compare them with the effects of acetylcholine.
  
  
  BACKGROUND
  Coronary endothelial dysfunction has been reported to occur early after heart transplantation, most notably when acetylcholine was the endothelium-function marker used. The effects of bradykinin on coronary vasomotion are unknown in HTXs.
  
  
  METHODS
  Sixteen HTXs were compared 3.6 +/- 1.7 months after transplantation to seven control subjects. Coronary flow velocity was measured using guide-wire Doppler. Diameters (D) of three segments of the left coronary artery and coronary blood flow (CBF) were assessed at baseline, after 3-min infusions of increasing bradykinin doses (50, 150 and 250 ng/min) then of increasing acetylcholine doses (estimated blood concentrations of 10(-8), 10(-7) and 10(-6) M).
  
  
  RESULTS
  Bradykinin induced similar dose-dependent increases in D and CBF in both groups: D was 11 +/- 12%, 19 +/- 14% and 22 +/- 16% (all p < 0.0001), and CBF was 50 +/- 40%, 130 +/- 68% and 186 +/- 77% (all p < 0.0001). Acetylcholine induced significant epicardial vasodilation in control subjects and vasoconstriction in HTX, as well as a marked increase in CBF in both groups. Acute allograft rejection, present in 8 of the 16 HTXs, did not modify responses to bradykinin, but was associated with a smaller CBF increase in response to acetylcholine (p < 0.05).
  
  
  CONCLUSIONS
  The coronary vasodilating effects of bradykinin are preserved early after heart transplantation, even in the presence of acute allograft rejection. Although there is an abnormal vasoconstricting response to acetylcholine reflecting endothelium dysfunction, the endothelium remains a functionally active organ in heart transplant recipients.
authors.affiliation: Fédération de Cardiologie et Institut National de la Santé et de la Recherche Médicale U400, Créteil, France. eaptecar@club-inernet.fr
authors.forename: E
authors.initials: E
authors.lastname: Aptecar
authors.forename: E
authors.initials: E
authors.lastname: Teiger
authors.forename: P
authors.initials: P
authors.lastname: Dupouy
authors.forename: C
authors.initials: C
authors.lastname: Benvenuti
authors.forename: M J
authors.initials: MJ
authors.lastname: Kern
authors.forename: J
authors.initials: J
authors.lastname: Woscoboinik
authors.forename: S
authors.initials: S
authors.lastname: Sediame
authors.forename: J M
authors.initials: JM
authors.lastname: Pernes
authors.forename: A
authors.initials: A
authors.lastname: Castaigne
authors.forename: D
authors.initials: D
authors.lastname: Loisance
authors.forename: J L
authors.initials: JL
authors.lastname: Dubois-Randé
mesh_terms: D000109:Acetylcholine; D000328:Adult; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D001920:Bradykinin; D002932:Cineangiography; D003326:Coronary Circulation / Q000187:drug effects* / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis* / Q000503:physiopathology; D016027:Heart Transplantation / Q000502:physiology*; D006801:Humans; D017078:Laser-Doppler Flowmetry; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D014655:Vascular Resistance / Q000187:drug effects* / Q000502:physiology
chemical_list: D000109:Acetylcholine; D001920:Bradykinin