Toward catalytically active oligonucleotides: synthesis of a flavin nucleotide and its incorporation into DNA.
[reaction--see text] The synthesis of a vitamin B(2)-derived flavin-nucleotide is described. A combined H-phosphonate/phosphoramidite protocol was developed for the first incorporation of flavin coenzymes into a DNA stack. The coenzyme-DNA is predicted to have novel biosensing and catalytic properties.
Schwögler
A
A
Department of Chemistry, Philipps-University Marburg, Germany.
Carell
T
T
D001483:Base Sequence; D015374:Biosensing Techniques; D002384:Catalysis; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D005415:Flavins / Q000737:chemistry*; D006860:Hydrogen Bonding; D007202:Indicators and Reagents; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012256:Riboflavin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005415:Flavins; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D012256:Riboflavin