title: Angular reconstitution of the Staphylothermus marinus phosphoenolpyruvate synthase.
abstract: The processes of single particle electron crystallography and three-dimensional angular reconstitution are applied to digital cryoelectron images of a macromolecular complex, the Staphylothermus marinus phosphoenolpyruvate synthase. In particular, the application of IQAD (iterative quaternionic angular determination) is exemplified in the context of more canonical approaches.
authors.affiliation: Department of Molecular Biology and Genetics, and Biophysics Interdepartmental Group, University of Guelph, Guelph, Ontario, Canada, N1G 2W1.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: F P
authors.initials: FP
authors.lastname: Ottensmeyer
authors.forename: G
authors.initials: G
authors.lastname: Harauz
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D019620:Desulfurococcaceae / Q000201:enzymology / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008958:Models, Molecular; D017866:Phosphotransferases (Paired Acceptors) / Q000648:ultrastructure*; D011487:Protein Conformation
chemical_list: D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase