Regulation of activator protein-1-DNA binding activity by opioid peptides in estrogen-sensitive cells of rat hypothalamus and uterus.
The present studies demonstrate, for the first time, that the binding of activator protein-1 (AP-1)-DNA in rat uterus and the estrogen-sensitive areas of the hypothalamus, as measured by electrophoretic mobility shift assay, is increased 2 h after intraperitoneal injection of [D-Met(2),Pro(5)]enkephalinamide. The effect was prevented by the opiate antagonist naltrexone given 30 min before the administration of [D-Met(2),Pro(5)]enkephalinamide, suggesting the involvement of opioid peptide receptors in the observed effects. The present findings support the role of opioid peptides in the regulation of transcription in estrogen-sensitive cells.
Oszter
A
A
Institute of Physiology, University Medical School of Pécs, Pécs, Hungary. oszter@physiol.pote.hu
Törocsik
B
B
Vértes
Z
Z
Környei
J L
JL
Kovács
K A
KA
Vértes
M
M
D000704:Analysis of Variance; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D018847:Opioid Peptides / Q000494:pharmacology*; D051381:Rats; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D004967:Estrogens; D018847:Opioid Peptides; D018808:Transcription Factor AP-1; D004247:DNA