Expression of Bacillus subtilis phytase in Lactobacillus plantarum 755.
Phytase enzymes can increase the nutritional value of food and feed by liberating inorganic phosphate from phytate, the major storage form of phosphorus in plants. The phytase (phyC) from Bacillus subtilis VTT E-68013 was expressed in Lactobacillus plantarum strain 755 using Lact. amylovorus alpha-amylase secretion signals. In an overnight cultivation in MRS medium containing cellobiose for induction of the alpha-amylase promoter, catalytically active phytase was secreted as a predominant extracellular protein. However, Western blot analysis revealed unprocessed and processed phytase in the cell fraction. Pulse chase experiments showed that the recombinant phytase was secreted at a slower rate in comparison to the native proteins of Lact. plantarum 755.
Kerovuo
J
J
Danisco Finland, Technology Center, Kantvik and Valio Ltd, Research and Development Center, Helsinki, Finland. janne.kerovuo@cultor.com
Tynkkynen
S
S
D010832:6-Phytase / Q000235:genetics* / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism
D011994:Recombinant Proteins; D010832:6-Phytase