Common antigenicity between Japanese cedar (Cryptomeria japonica) pollen and Japanese cypress (Chamaecyparis obtusa) pollen, II. Determination of the cross-reacting T-cell epitope of cry j 1 and cha o 1 in mice.
We have previously detected common antigenicity between Cry j 1 and Cha o 1 in B10.S mice. B10.S mice immunized with Cry j 1- or Cha o 1-generated T cells and antibodies reactive to both allergens. In the present study, we investigated the cross-reacting and Cry j 1-specific T-cell epitopes in B10.S mice. Lymph node cells from B10. S mice immunized with Cry j 1 recognized Cry j 1 p111-130, p211-230, and p310-330 as well as Cha o 1 p209-228. The existence of the cross-reacting T-cell epitope in Cry j 1 and Cha o 1 was confirmed by the response of newly established p211-230-specific and Cha o 1 p209-228-specific T-cell lines. The minimum peptide sequence (p213-224) of the cross-reacting T-cell epitope was identical in Cry j 1 and Cha o 1. These findings clearly demonstrate that common antigenicity at the T-cell level between Japanese cedar and cypress pollen allergens was caused by the existence of an identitical-cell epitope in Cry j 1 and Cha o 1.
Ohno
N
N
Department of Pediatrics, Institute of DNA Medicine, The Jikei University School of Medicine, Tokyo, Japan.
Ide
T
T
Sakaguchi
M
M
Inouye
S
S
Saito
S
S
D000485:Allergens / Q000276:immunology*; D000818:Animals; D052179:Antigens, Plant; D002460:Cell Line; D003429:Cross Reactions; D018604:Epitope Mapping / Q000379:methods; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010940:Plant Proteins / Q000008:administration & dosage / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014197:Trees
D000485:Allergens; D052179:Antigens, Plant; C408445:Cha o 1 protein, Chamaecyparis obtusa; C065290:Cry j I protein, Cryptomeria japonica; D000939:Epitopes; D006183:H-2 Antigens; D010940:Plant Proteins