Inhibitory effect of flavonoids from citrus plants on Epstein-Barr virus activation and two-stage carcinogenesis of skin tumors.
To search for possible anti-tumor promoters, thirteen flavones (1-13) obtained from the peel of Citrus plants were examined for their inhibitory effects on the Epstein-Barr virus early antigen (EBV-EA) activation by a short-term in vitro assay. Of these flavones, 3,5,6,7,8,3',4'-heptamethoxyflavone (HPT) (13) exhibited significant inhibitory effects on the EBV-EA activation induced by the tumor promoter, 12-O-tetradecanoylphorbol 13-acetate (TPA). Further, compound 13 exhibited remarkable inhibitory effects on mouse skin tumor promotion in an in vivo two-stage carcinogenesis test.
Iwase
Y
Y
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyubancho, Nishinomiya, Hyogo, Japan.
Takemura
Y
Y
Ju-ichi
M
M
Ito
C
C
Furukawa
H
H
Kawaii
S
S
Yano
M
M
Mou
X Y
XY
Takayasu
J
J
Tokuda
H
H
Nishino
H
H
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism*; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010212:Papilloma / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate; D013997:Time Factors; D014775:Virus Activation / Q000187:drug effects*
D000956:Antigens, Viral; C033305:Epstein-Barr virus early antigen; D005419:Flavonoids; D010936:Plant Extracts; C059294:3,3',4',5,6,7,8-heptamethoxyflavone; D013755:Tetradecanoylphorbol Acetate