title: Carbazole as an excited state proton transfer fluorescent probe for lipid bilayers in alkaline medium.
abstract: In our effort to look for novel excited state proton transfer (ESPT) fluorescent probes in alkaline pH range, we have examined carbazole as a possible candidate because of its high extinction coefficient, high quantum yield and a larger difference in ionization constant between the ground and excited state (pKa - pKa*). The photodissociation of carbazole was studied in liposome membrane by steady state fluorescence measurements at alkaline pH ranges. The neutral form and the anionic form of carbazole emit at 362 and 417 nm, respectively. This large shift in emission makes it convenient to monitor the physical properties of liposomes. The neutral form fluorescence intensity of carbazole is sensitive to phase changes in the membrane and also shows a maximum at phase transition temperature. This variation of intensity can be explained in terms of redistribution of probe between the surface and interior of the liposomes. Cholesterol induced phase changes of liposomes were also sensed by the ESPT of carbazole.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Madras.
authors.forename: N
authors.initials: N
authors.lastname: Pappayee
authors.forename: A K
authors.initials: AK
authors.lastname: Mishra
mesh_terms: D000468:Alkalies; D002227:Carbazoles; D002784:Cholesterol; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D011522:Protons; D013696:Temperature
chemical_list: D000468:Alkalies; D002227:Carbazoles; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D011522:Protons; C041514:carbazole; D002784:Cholesterol