Molecular genetic applications of streptavidin-coated manifold supports.
Practical problems of handling large numbers of samples limit the application of molecular genetic procedures in clinical settings and in research. In the present review we describe a multipronged manifold support, coated with streptavidin, that offers distinct advantages in preparative and diagnostic applications. In order to increase the surface available on the manifold, porous Sepharose particles conjugated with streptavidin were attached to the plastic support. This procedure increased the surface by almost three orders of magnitude, permitting sufficient streptavidin to be coupled to the support for most routine applications. The manifold supports have been used for sample preparation and in a number of genetic assays, including allele discrimination assays and DNA sequencing, In all these assay formats the manifold supports allow large numbers of samples to be processed in parallel.
Barbany
G
G
Department of Medical Genetics, Uppsala Biomedical Centre, University of Uppsala, Sweden.
Hagberg
A
A
Waldenström
E
E
Landegren
U
U
D018076:DNA, Complementary / Q000235:genetics; D005784:Gene Amplification; D006801:Humans; D007700:Kinetics; D008674:Metals, Rare Earth; D008855:Microscopy, Electron, Scanning; D008967:Molecular Biology / Q000379:methods*; D011485:Protein Binding; D015202:Protein Engineering; D012333:RNA, Messenger / Q000302:isolation & purification; D017422:Sequence Analysis, DNA / Q000379:methods; D019809:Streptavidin; D013499:Surface Properties
D018076:DNA, Complementary; D008674:Metals, Rare Earth; D012333:RNA, Messenger; D019809:Streptavidin