title: Live oral poliovirus vaccines do not contain detectable simian virus 40 (SV40) DNA.
abstract: Prior to 1962, poliovirus vaccines produced in rhesus monkey kidney cells were contaminated with SV40. Recent studies reporting the detection of SV40 in human tumours raised concern that SV40 may be oncogenic in humans. To provide further assurance that currently used poliovirus vaccines are not contaminated with SV40, we used the polymerase chain reaction (PCR) to search for SV40 DNA in live oral poliovirus vaccines manufactured in the United States between 1972 and 1996. SV40 DNA sequences were not found in any of the vaccine lots tested.
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
authors.forename: A
authors.initials: A
authors.lastname: Sierra-Honigmann
authors.forename: P R
authors.initials: PR
authors.lastname: Krause
mesh_terms: D003257:Consumer Product Safety; D004279:DNA, Viral / Q000032:analysis*; D004340:Drug Contamination; D006801:Humans; D011055:Poliovirus Vaccine, Oral; D013539:Simian virus 40 / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral; D011055:Poliovirus Vaccine, Oral