title: IL-12 induces SDS-stable class II alphabeta dimers in murine dendritic cells.
abstract: We assessed the effect of rIL-12 on the expression of class II molecules and on the ratio between SDS-stable and unstable alphabeta dimers in dendritic cells. We found that in vitro exposure of the cells to IL-12 increased their surface expression of mature class II molecules, despite a marked decline in class II biosynthesis. This effect was accompanied by a striking increase in the overall proportion of SDS-stable heterodimers.
authors.affiliation: Department of Experimental Medicine, University of Perugia, Italy. grohmann@tin.it
authors.forename: U
authors.initials: U
authors.lastname: Grohmann
authors.forename: C
authors.initials: C
authors.lastname: Orabona
authors.forename: R
authors.initials: R
authors.lastname: Bianchi
authors.forename: M L
authors.initials: ML
authors.lastname: Belladonna
authors.forename: M C
authors.initials: MC
authors.lastname: Fioretti
authors.forename: P
authors.initials: P
authors.lastname: Puccetti
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D019281:Dimerization; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D008811:Mice, Inbred DBA; D012967:Sodium Dodecyl Sulfate
chemical_list: D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; D018664:Interleukin-12; D012967:Sodium Dodecyl Sulfate