Effect of L-methioninyl adenylate on the level of aminoacylation in vivo of tRNA(Met) from Escherichia coli K12.
In cells of E.coli K12 grown exponentially in minimal medium, tRNA(met), tRNA(leu) and tRNA(ile) are aminoacylated at 100%, 80% and 64%, respectively. On addition of L-methioninyl adenylate to the growth medium, one observes a specific deacylation of tRNA(met). When more than 35% of tRNA(met) is deacylated, growth rate is reduced and becomes proportional to the amount of methionyl-tRNA formed.
Cassio
D
D
Mathien
Y
Y
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D012329:RNA, Bacterial / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012355:RNA, Transfer, Ile / Q000378:metabolism; D012356:RNA, Transfer, Leu / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000378:metabolism*
D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012355:RNA, Transfer, Ile; D012356:RNA, Transfer, Leu; D012358:RNA, Transfer, Met; C011501:methioninyl adenylate; D000249:Adenosine Monophosphate; D008715:Methionine