Sequence and organization analyses of a Zygosaccharomyces rouxii DNA fragment containing the HIS3 gene.
The nucleotide sequence of a 3.4 kb fragment containing the HIS3 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The fragment was cloned from a Z. rouxii genomic DNA library by complementation of a Saccharomyces cerevisiae his3 mutant strain. The sequenced DNA fragment contained three open reading frames; the middle one (678 bp long, predicting a protein of 226 amino acids) shared a high degree of similarity with HIS3 genes of other yeast species. In the promoter region of the putative ZrHIS3 gene, a T(c) element required for constitutive transcription was found. The GenBank Accession No. of the sequenced DNA region is Y18561.
Sychrova
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H
Department of Membrane Transport, Institute of Physiology CzAcadSci, 142 20 Prague 4, Czech Republic. sychrova@biomed.cas.cz
Braun
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Souciet
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JL
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D015723:Gene Library; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*
D004271:DNA, Fungal; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase