title: Double-stranded DNA liquid-crystalline dispersions as biosensing units.
abstract: Three different approaches to constructing biosensing units based on double-stranded (ds) DNA molecules, capable of detecting various biologically active compounds, are considered. The first approach is based on the abnormal optical activity of the liquid-crystalline dispersion formed from ds DNA molecules, modified by relevant physical factors or treated with biologically active compounds. The second one is based on the abnormal optical activity of the liquid-crystalline dispersions formed first from the ds DNA and then treated with coloured biologically active compounds. The third one is based on the abnormal optical activity, specific to particles of the liquid-crystalline dispersions, where the neighbouring DNA molecules are crosslinked by artificial polymeric bridges. These approaches permit the detection of biologically relevant compounds of various origins.
authors.affiliation: Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Moscow.
authors.forename: Y M
authors.initials: YM
authors.lastname: Yevdokimov
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011108:Polymers / Q000737:chemistry
chemical_list: D011108:Polymers; D004247:DNA