[Dark metabolism of acetate in Rhodospirillum rubrum cells, grown under photoheterotropic conditions].
The mechanism of the dark assimilation of acetate in the photoheterotrophically grown nonsulfur bacterium Rhodospirillum rubrum was studied. Both in the light and in the dark, acetate assimilation in Rsp. rubrum cells, which lack the glyoxylate pathway, was accompanied by the excretion of glyoxylate into the growth medium. The assimilation of propionate was accompanied by the excretion of pyruvate. Acetate assimilation was found to be stimulated by bicarbonate, pyruvate, the C4-dicarboxylic acids of the Krebs cycle, and glyoxylate, but not by propionate. These data implied that the citramalate (CM) cycle in Rsp. rubrum cells grown aerobically in the dark can function as an anaplerotic pathway. This supposition was confirmed by respiration measurements. The respiration of cells oxidizing acetate depended on the presence of CO2 in the medium. The fact that the intermediates of the CM cycle (citramalate and mesaconate) markedly inhibited acetate assimilation but had almost no effect on cell respiration indicative that citramalate and mesaconate are intermediates of the acetate assimilation pathway. The inhibition of acetate assimilation and cell respiration by itaconate was due to its inhibitory effect on propionyl-CoA carboxylase, an enzyme of the CM cycle. The addition of 5 mM itaconate to extracts of Rsp. rubrum cells inhibited the activity of this enzyme by 85%. The data obtained suggest that the CM cycle continues to function in Rsp. rubrum cells that have been grown anaerobically in the light and then transferred to the dark and incubated aerobically.
Berg
I A
IA
Department of Microbiology, Biological Faculty, Moscow State University, Vorob'evy gory, Russia.
Krasil'nikova
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EN
Ivanovskiĭ
R N
RN
D000085:Acetates / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors; D003470:Culture Media; D003624:Darkness; D008027:Light; D043586:Methylmalonyl-CoA Decarboxylase; D012247:Rhodospirillum rubrum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects
D000085:Acetates; D003470:Culture Media; D002262:Carboxy-Lyases; D043586:Methylmalonyl-CoA Decarboxylase