title: Aberrant proteolytic digestion of biglycan and decorin by saliva and exocrine gland lysates from the NOD mouse model for autoimmune exocrinopathy.
abstract: OBJECTIVE
  The protein components of the extracellular matrix (ECM) are responsible for driving tissue morphogenesis, the development of differentiated function, and the sequestration of biologically active molecules such as growth factors in close proximity to tissue and organ cells. Recent reports indicate that saliva and exocrine tissue lysates from Sjögren's syndrome patients and the non-obese diabetic (NOD) mouse model for autoimmune exocrinopathy demonstrate elevated levels of specific enzymes that degrade the ECM, especially the matrix metalloproteinases (MMPs). To determine if elevated levels of MMPs could be important in exocrine tissue destruction, we examined proteolytic activity against two ECM proteoglycans, decorin and biglycan.
  
  
  METHODS
  Purified recombinant human core protein for decorin or biglycan was incubated with saliva or gland lysates from either control BALB/c or NOD mice. Degraded proteoglycan products were estimated by Western blotting analysis using anti-decorin or anti-biglycan monospecific polyclonal antibodies. The levels of TGF beta protein were measured by ELISA.
  
  
  RESULTS
  Proteolytic activity for decorin and biglycan was not observed in the saliva and salivary gland lysates collected from C57BL/6 or BALB/c mice used as normal controls. In contrast, both proteoglycans were degraded by saliva and exocrine gland lysates from NOD mice and the congenic partner strains NOD-scid and NOD.B10.H2b. This proteolytic activity for proteoglycans was inhibited by the MMP inhibitors, EDTA, GM6001 and 1,10-phenanthroline. Protein steady state levels for TGF beta were increased in the saliva and gland lysates from 20-week old NOD strains, as compared to BALB/c mice and NOD treated with the MMP inhibitor GM6001. With the inhibition of MMP activity, TGF beta levels declined in saliva and gland lysates.
  
  
  CONCLUSIONS
  Proteolytic degradation of the ECM molecules decorin and biglycan is elevated in the exocrine tissues of the NOD mouse model for Sjögren's syndrome. Furthermore, the proteolysis of small leucine-rich proteoglycans correlates with the presence of elevated levels of TGF beta in gland lysates and saliva.
authors.affiliation: Department of Oral Biology, University of Florida, Gainesville, USA.
authors.forename: S
authors.initials: S
authors.lastname: Yamachika
authors.forename: J
authors.initials: J
authors.lastname: Brayer
authors.forename: G E
authors.initials: GE
authors.lastname: Oxford
authors.forename: A B
authors.initials: AB
authors.lastname: Peck
authors.forename: M G
authors.initials: MG
authors.lastname: Humphreys-Beher
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000209:etiology; D058578:Biglycan; D015153:Blotting, Western; D058575:Decorin; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D011509:Proteoglycans / Q000378:metabolism*; D011994:Recombinant Proteins; D012463:Saliva / Q000201:enzymology*; D012469:Salivary Glands / Q000201:enzymology* / Q000378:metabolism; D016511:Severe Combined Immunodeficiency / Q000201:enzymology / Q000235:genetics; D012793:Sialadenitis / Q000201:enzymology; D012859:Sjogren's Syndrome / Q000201:enzymology; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: C546884:BGN protein, human; C546885:Bgn protein, mouse; D058578:Biglycan; C546878:DCN protein, human; C546879:Dcn protein, mouse; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D020782:Matrix Metalloproteinases