A retroviral vector capable of targeted gene transfer into cells expressing HIV envelope glycoprotein.
An expression vector encoding a chimeric envelope protein composed of CD4 and ecotropic retroviral envelope glycoprotein was constructed with the aim of accomplishing targeted gene transfer into HIV-1-infected cells. The chimeric protein was efficiently expressed and transported to the surfaces of various cell types and supported HIV-1 entry into human cells. A packaging cell line producing retroviral vectors carrying chimeric envelope proteins was then established. The vector particles produced were shown to be capable of specific gene transfer into human cells expressing HIV envelope glycoprotein. Blocking experiments confirmed that the vector particles entered the cells via an interaction between the chimeric and HIV envelope proteins. This targeting vector may thus be a useful tool with which to develop effective gene therapies against HIV infection.
Suzuki
S
S
Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, 113-8602, Japan.
Shimada
T
T
D000818:Animals; D015704:CD4 Antigens / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D005434:Flow Cytometry; D015967:Gene Expression Regulation, Viral; D018390:Gene Targeting; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D015497:HIV-1 / Q000378:metabolism* / Q000472:pathogenicity; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D011991:Receptors, Virus / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism*
D015704:CD4 Antigens; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C070506:ecotropic murine leukemia virus receptor