title: [The synthesis of a cobalt(II) tetracarboxyphthalocyanine- deoxyribooligonucleotide conjugate as a reagent for the directed DNA modification].
abstract: The cobalt(II) tetracarboxyphthalocyanine-deoxyribonucleotide pd(TCTTCCCA) conjugate was synthesized. The phthalocyanine N-succinimide ester prepared from phthalocyanine using DCC was mixed in DMF with an aqueous solution of the oligonucleotide bearing a 1,3-diaminopropane linker at the 5'-phosphate. The resulting conjugate was tested in the intraduplex reaction with target 14-mer and 22-mer oligonucleotides containing conjugate-complementary sequences. In the presence of O2 and a thiol (2-mercaptoethanol or DTT) as a coupled reducer or H2O2, sequence-specific DNA modification was observed that caused the cleavage of the target upon treatment with piperidine.
authors.affiliation: Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Koval'
authors.forename: A A
authors.initials: AA
authors.lastname: Chernonosov
authors.forename: T V
authors.initials: TV
authors.lastname: Abramova
authors.forename: T M
authors.initials: TM
authors.lastname: Ivanova
authors.forename: O S
authors.initials: OS
authors.lastname: Fedorova
authors.forename: D G
authors.initials: DG
authors.lastname: Knorre
mesh_terms: D000818:Animals; D003035:Cobalt; D004247:DNA / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007211:Indoles / Q000138:chemical synthesis* / Q000737:chemistry*; D054833:Isoindoles; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D007211:Indoles; D054833:Isoindoles; D009841:Oligonucleotides; D003035:Cobalt; D004247:DNA; C013647:phthalocyanine