title: Skin prick test and serological analysis with recombinant group 2 allergens of the dust mites L. destructor and T. putrescentiae.
abstract: BACKGROUND
  The dust mites Lepidoglyphus destructor and Tyrophagus putrescentiae are important sources of allergen in farming environments. The major allergens of the dust mites L. destructor and T. putrescentiae have been cloned and expressed as recombinant proteins.
  
  
  OBJECTIVE
  To evaluate the use of recombinant group 2 allergens of L. destructor (rLep d 2) and T. putrescentiae (rTyr p 2) in skin prick test (SPT), and serological analysis in sensitized and non-sensitized farmers chronically exposed to dust mites.
  
  
  METHODS
  Skin prick test with rLep d 2, rTyr p 2 and the corresponding commercial extracts was performed in 44 farmers sensitized to L. destructor and/or T. putrescentiae, and 38 control farmers. IgE and IgG subclass antibodies to the recombinant allergens were analysed by RAST and ELISA, respectively.
  
  
  RESULTS
  Out of the 44 subjects positive in SPT to L. destructor and/or T. putrescentiae extract, 26 (59%) displayed a positive SPT to one or the other of the recombinant allergens, whereas 21 (48%) were positive to both. Significant correlations were registered between the sizes of the weals induced by rLep d 2 and rTyr p 2 and the corresponding RAST values (P < 0.001). A majority of subjects positive in SPT to the recombinant allergens had detectable IgG4 antibodies, and the levels were significantly higher in the dust mite sensitized group than in the controls (P < 0.05). No such differences were found in the IgG1 values (P > 0.05). The results obtained with rLep d 2 and rTyr p 2 correlated relatively well with each other with respect to SPT, RAST and IgG4, suggesting that the allergens have similar or shared IgE epitopes. All the control subjects had a negative SPT and RAST to rLep d 2 and rTyr p 2.
  
  
  CONCLUSIONS
  Recombinant group 2 allergens from the dust mite L. destructor and T. putrescentiae represent useful tools for diagnosis of dust mite allergy.
authors.affiliation: Department of Medicine, Danderyd Hospital, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Kronqvist
authors.forename: E
authors.initials: E
authors.lastname: Johansson
authors.forename: C G
authors.initials: CG
authors.lastname: Magnusson
authors.forename: S
authors.initials: S
authors.lastname: Olsson
authors.forename: T L
authors.initials: TL
authors.lastname: Eriksson
authors.forename: G
authors.initials: G
authors.lastname: Gafvelin
authors.forename: M
authors.initials: M
authors.lastname: van Hage-Hamsten
mesh_terms: D000328:Adult; D000368:Aged; D000485:Allergens / Q000097:blood; D000818:Animals; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D007136:Immunoglobulins / Q000096:biosynthesis / Q000097:blood; D008875:Middle Aged; D008925:Mites / Q000276:immunology*; D011506:Proteins / Q000032:analysis / Q000276:immunology; D011994:Recombinant Proteins / Q000097:blood; D012698:Serologic Tests; D012882:Skin Tests
chemical_list: D000485:Allergens; D007136:Immunoglobulins; C090039:Lep d I allergen; D011506:Proteins; D011994:Recombinant Proteins; C110921:Tyr p 2 protein, Tyrophagus putrescentiae