[Synthesis and fluorescent properties of 5-(1-pyrenylethynyl)-2'- deoxyuridine-containing oligodeoxynucleotides].
Novel reagents for the fluorescent labeling of oligo- and polynucleotides have been prepared: 5-(1-pyrenylethynyl)-2'-deoxyuridine 3'-phosphoramidite and a solid support carrying this nucleoside. Oligonucleotides containing one or several modified units have been synthesized, and the fluorescence of these probes has been shown to change upon hybridization with the complementary sequence.
Malakhov
A D
AD
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Malakhova
E V
EV
Kyznitsova
S V
SV
Grechishnikova
I V
IV
Prokhorenko
I A
IA
Skorobogatyĭ
M V
MV
Korshun
V A
VA
Berlin
Iu A
IuA
D005456:Fluorescent Dyes / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
C410922:5-(pyrenylethynyl)-2'-deoxyuridine; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D011721:Pyrenes; D014529:Uridine