title: Chemiluminescent immunodetection protocols with 1,2-dioxetane substrates. 4.
abstract: Chemiluminescent 1,2-dioxetane enzyme substrates provide a highly sensitive and versatile detection method for immunoblots and other membrane-based detections. 1,2-Dioxetane substrates, coupled with either alkaline phosphatase or beta-galactosidase enzyme labels, generate glow light emission kinetics, with a signal duration that is significantly longer than most enhanced luminol/horseradish peroxidase chemiluminescent detection systems. The long-lived, high-intensity light signal is ideal for imaging using a variety of formats, including X-ray film, photographic film, chemiluminescence phosphor imaging screens, and the rapidly expanding selection of camera imaging systems.
authors.affiliation: Tropix, Inc., Bedford, Massachusetts 01730, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Olesen
authors.forename: J
authors.initials: J
authors.lastname: Mosier
authors.forename: J C
authors.initials: JC
authors.lastname: Voyta
authors.forename: I
authors.initials: I
authors.lastname: Bronstein
mesh_terms: D015153:Blotting, Western / Q000379:methods*; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring; D008163:Luminescent Measurements
chemical_list: D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; C079142:1,2-dioxetane