title: Calpain-3 gene expression is decreased during experimental cancer cachexia.
abstract: The Yoshida AH-130 rat ascites hepatoma is a model system for studying the mechanisms involved in the protein hypercatabolism associated with cancer cachexia. The present study was aimed at investigating if the calpain-3 gene expression in skeletal muscle was affected by tumor growth. The results presented clearly show that calpain-3 gene expression is considerably reduced in experimental cancer cachexia, while there is a reciprocal change in the expression of the ubiquitin-dependent proteolytic system and in the ubiquitous m-calpain. The results, observed during cancer cachexia, suggest a potential counterregulatory role of calpain-3 in muscle proteolysis.
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08071 Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Busquets
authors.forename: C
authors.initials: C
authors.lastname: García-Martínez
authors.forename: B
authors.initials: B
authors.lastname: Alvarez
authors.forename: N
authors.initials: N
authors.lastname: Carbó
authors.forename: F J
authors.initials: FJ
authors.lastname: López-Soriano
authors.forename: J M
authors.initials: JM
authors.lastname: Argilés
mesh_terms: D000818:Animals; D002100:Cachexia / Q000201:enzymology* / Q000209:etiology; D002154:Calpain / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology*; D009374:Neoplasms, Experimental / Q000150:complications; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015342:DNA Probes; D009124:Muscle Proteins; D012313:RNA; D002154:Calpain