title: Erythropoietin assay in mice made polycythemic by transfusion of heterologous red cells.
abstract: A simple in vivo bioassay suitable for routine testing of quality control of recombinant human erythropoietin (rHu-EPO) analogues was developed. Mice made polycythemic by intraperitoneal injection of 1.2 ml of a 80% suspension of heterologous (rat) red cells were used as assay animals and splenic 59Fe uptake as expression of the response to rHu-EPO. The assay took three days and the following schedule is proposed: 1) intraperitoneal injection of 1.2 ml of washed packed red cells obtained from donor rats, 2) subcutaneous injection of test material 4-5 h after transfusion, 3) intravenous administration of 59Fe tracer 48 h later, and 4) determination of splenic isotope uptake 6 h after injection. This method for the in vivo bioassay of rHu-EPO analogues is an economical and reliable alternative to the existing bioassays of the hormone.
authors.affiliation: Cátedra de Fisiología, Facultad de Odontología, Universidad de Buenos Aires, Argentina.
authors.forename: M I
authors.initials: MI
authors.lastname: Conti
authors.forename: M P
authors.initials: MP
authors.lastname: Martínez
authors.forename: A C
authors.initials: AC
authors.lastname: Barceló
authors.forename: R M
authors.initials: RM
authors.lastname: Alippi
authors.forename: C E
authors.initials: CE
authors.lastname: Bozzini
mesh_terms: D000818:Animals; D001681:Biological Assay; D017707:Erythrocyte Transfusion; D004920:Erythropoiesis; D004921:Erythropoietin / Q000032:analysis*; D005260:Female; D007504:Iron Radioisotopes; D051379:Mice; D011086:Polycythemia / Q000209:etiology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins
chemical_list: D007504:Iron Radioisotopes; D011994:Recombinant Proteins; D004921:Erythropoietin