title: Isopentenyl diphosphate isomerase deficiency in Synechocystis sp. strain PCC6803.
abstract: Isopentenyl diphosphate isomerase (IPP isomerase) in many organisms and in plastids is central to isoprenoid synthesis and involves the conversion between IPP and dimethylallyl diphosphate (DMAPP). It is shown that Synechocystis PCC6803 is deficient in IPP isomerase activity, consistent with the absence in its genome of an obvious homologue for the enzyme. Incorporation of [1-(14)C]IPP in cell extracts, primarily into C(20), occurs only upon priming with DMAPP in Synechocystis PCC6803 and in Synechococcus PCC7942. Isoprenoid synthesis in these cyanobacteria does not appear to involve interconversion of IPP and DMAPP, raising the possibility that they are not within the plastid evolutionary lineage.
authors.affiliation: Department of Cell Biology and Molecular Genetics, University of Maryland, 20742, College Park, MD 20742, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Ershov
authors.forename: R R
authors.initials: RR
authors.lastname: Gantt
authors.forename: F X
authors.initials: FX
authors.lastname: Cunningham
authors.forename: E
authors.initials: E
authors.lastname: Gantt
mesh_terms: D019748:Carbon-Carbon Double Bond Isomerases / Q000737:chemistry / Q000172:deficiency*; D000460:Chlorophyta / Q000201:enzymology; D002853:Chromatography, Liquid; D000458:Cyanobacteria / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology; D045782:Hemiterpenes; D009943:Organophosphorus Compounds / Q000378:metabolism / Q000494:pharmacology; D013729:Terpenes / Q000378:metabolism
chemical_list: D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D013729:Terpenes; C043060:3,3-dimethylallyl pyrophosphate; D019748:Carbon-Carbon Double Bond Isomerases; C021453:isopentenyldiphosphate delta-isomerase