title: In vitro potency assay for yellow fever vaccines: comparison of three vero cell lines sources.
abstract: Quality control of Yellow Fever vaccines performed by Control Authorities prior to marketing vaccines batches requires in vitro potency assays. The two currently available methods are the plaque formation assay and the cytopathic effect assay based on the use of porcine kidney PS cells or monkey kidney Vero cells. Among several sources of variation in virus titration, the cell systems are considered as important issues and Quality Assurance strongly recommends working with cell banks from certified suppliers. The aim of our study was to compare the behaviour and the sensitivity of three Vero cell sources obtained from ATCC, WHO and EP used at different passage levels in a plaque formation test. The conclusion of this work was that the yellow fever live attenuated virus titration, adapted in Vero cell lines appeared as a reliable method applicable for routine in vitro potency assay. The comparison of Vero cell lines, originated from three different sources, showed that they could be equally used as substrates by laboratories having the basic facility of cell culture, without influence on the final viral titre.
authors.affiliation: Agence Française de Sécurité Sanitaire des Produits de Santé, Direction des Laboratoires et des Contrôles, Lyon, France.
authors.forename: J
authors.initials: J
authors.lastname: Fournier-Caruana
authors.forename: B
authors.initials: B
authors.lastname: Poirier
authors.forename: F
authors.initials: F
authors.lastname: Garnier
authors.forename: F
authors.initials: F
authors.lastname: Fuchs
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003257:Consumer Product Safety; D011786:Quality Control; D014613:Vaccines, Attenuated; D014709:Vero Cells; D010948:Viral Plaque Assay; D014765:Viral Vaccines; D015005:Yellow fever virus / Q000472:pathogenicity*
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines