Enrichment of lineage-CD34- cells using a newly developed filter system.
Recent studies have demonstrated the presence of human lineage- (lin-)CD34- cells that are capable of differentiating into CD34+ cells in xenogeneic transplantation systems. We developed a new filter system that can enrich lin-CD34- cells, a precursor cell population of CD34+ cells. The filter consists of polyethylene terephthalate non-woven fabrics coated with hydrophilic polymer. The frequency of lin-CD34- cells in the cell population after filtration was 7.45 +/- 4.41%, a 16.8 +/- 8.81-fold enrichment compared with 0. 49 +/- 0.31% in the cell population before filtration. The mean recovery of lin-CD34- cells was 48.57 +/- 13.59% (n = 15). Purified lin-CD34- cells, obtained by sorting the filtrated cell population, acquired CD34 expression and colony-forming activity after 7 d of culture. Our results indicate that this filter system is useful for isolating lin-CD34- cells, including precursors of CD34+ cells, and will help further the study of lin-CD34- cells.
Nakamura
Y
Y
Research Centre for Genetic Engineering and Cell Transplantation; Department of Haematology, Tokai University School of Medicine, Kanagawa.
Ando
K
K
Muguruma
Y
Y
Sumita
M
M
Terashima
S
S
Yoshida
M
M
Yamashita
Y
Y
Hotta
T
T
Kato
S
S
D018952:Antigens, CD34 / Q000276:immunology*; D018929:Cell Culture Techniques; D019070:Cell Lineage; D005434:Flow Cytometry / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006440:Hemofiltration / Q000379:methods*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018952:Antigens, CD34