title: Linear free energy relationships implicate three modes of binding for fluoroaromatic inhibitors to a mutant of carbonic anhydrase II.
abstract: [figure: see text] Linear free energy relationships between binding affinity and hydrophobicity for a library of fluoroaromatic inhibitors of F131V carbonic anhydrase II (CA) implicate three modes of interaction. X-ray crystal structures suggest that F131 interacts with fluoroaromatic inhibitors, while P202, on the opposite side of the active site cleft, serves as the site of the hydrophobic contact in the case of the F131V mutant. 2-Fluorinated compounds bind more tightly, perhaps due to the field effect of the nearby fluorine on the acidity of the amide proton.
authors.affiliation: Department of Chemistry, Swarthmore College, Pennsylvania 19081, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Doyon
authors.forename: E A
authors.initials: EA
authors.lastname: Hansen
authors.forename: C Y
authors.initials: CY
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Chang
authors.forename: D W
authors.initials: DW
authors.lastname: Christianson
authors.forename: R D
authors.initials: RD
authors.lastname: Madder
authors.forename: J G
authors.initials: JG
authors.lastname: Voet
authors.forename: T A
authors.initials: TA
authors.lastname: Baird
authors.forename: C A
authors.initials: CA
authors.lastname: Fierke
authors.forename: A
authors.initials: A
authors.lastname: Jain
mesh_terms: D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000235:genetics* / Q000378:metabolism*; D003461:Crystallography / Q000379:methods; D005461:Fluorine / Q000737:chemistry; D018499:Linear Energy Transfer; D008958:Models, Molecular; D009154:Mutation; D011487:Protein Conformation
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D005461:Fluorine; D002256:Carbonic Anhydrases