Calpain-3 gene expression is decreased during experimental cancer cachexia.
The Yoshida AH-130 rat ascites hepatoma is a model system for studying the mechanisms involved in the protein hypercatabolism associated with cancer cachexia. The present study was aimed at investigating if the calpain-3 gene expression in skeletal muscle was affected by tumor growth. The results presented clearly show that calpain-3 gene expression is considerably reduced in experimental cancer cachexia, while there is a reciprocal change in the expression of the ubiquitin-dependent proteolytic system and in the ubiquitous m-calpain. The results, observed during cancer cachexia, suggest a potential counterregulatory role of calpain-3 in muscle proteolysis.
Busquets
S
S
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08071 Barcelona, Spain.
García-Martínez
C
C
Alvarez
B
B
Carbó
N
N
López-Soriano
F J
FJ
Argilés
J M
JM
D000818:Animals; D002100:Cachexia / Q000201:enzymology* / Q000209:etiology; D002154:Calpain / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology*; D009374:Neoplasms, Experimental / Q000150:complications; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar
D015342:DNA Probes; D009124:Muscle Proteins; D012313:RNA; D002154:Calpain