Distribution of rotavirus VP4 genotypes and VP7 serotypes among nonhospitalized and hospitalized patients with gastroenteritis and patients with nosocomially acquired gastroenteritis in Austria.
To assess the potential benefits of a reassortant tetravalent rotavirus vaccine, we investigated stool specimens from children in three different groups by reverse transcription-PCR (RT-PCR) for rotavirus G and P types: (i) children not hospitalized with community-acquired rotavirus-acute gastroenteritis (RV-AGE), (ii) children hospitalized for RV-AGE, and (iii) children with nosocomially acquired RV-AGE. From a total of 553 samples investigated, 335 were positive by enzyme-linked immunosorbent assay, of which 294 (88%) were positive by RT-PCR. Among the RT-PCR-positive samples, the predominant types were G1P[8] (84%), followed by G4P[8] (9%) and G3P[8] (2%). No differences between the three groups were observed, suggesting that community vaccination will diminish the most cost-relevant cases of hospitalizations and nosocomial infections.
Frühwirth
M
M
Department of Pediatrics, University Hospital, Innsbruck, Austria. Martin.Fruehwirth@uibk.ac.at
Brösl
S
S
Ellemunter
H
H
Moll-Schüler
I
I
Rohwedder
A
A
Mutz
I
I
D001317:Austria; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002648:Child; D003428:Cross Infection / Q000821:virology*; D005759:Gastroenteritis / Q000821:virology*; D005838:Genotype; D006801:Humans; D007297:Inpatients; D010045:Outpatients; D016601:RNA-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012703:Serotyping; D017361:Viral Nonstructural Proteins / Q000235:genetics*
D036022:Capsid Proteins; D016601:RNA-Binding Proteins; C060339:VP4 protein, Rotavirus; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus