[Typing of methicillin resistant strains of S. haemolyticus isolated from patients and from the hospital ward environment].
Biotyping, antibiograms and fingerprinting were used to determine the relation of 16 methicillin-resistant S. haemolyticus isolated from drains in patients who underwent intraabdominal surgery to 9 methicillin-resistant strains of S. haemolyticus isolated at the same time from hospital environment. The comparison of biochemical properties of the examined strains showed a large variety of biochemical profiles as well as antibiotic patterns of susceptibility. The differences in sensitivity to the antibiotics used were not distinct. Biotyping and antibiograms did not permit determination of the relation of the investigated strains. Only the results of fingerprinting allowed for the division of the 25 examined strains into three genotypes demonstrating three main patterns of PCR products. 16 strains of 25 showed the same pattern of PCR products. This results suggests the presence of a source of infection on the clinical ward. A nurse may have been the source of infection because the same genotype of S. haemolyticus was isolated from her nasal anterior as from the majority of patients.
Chylak
J
J
Zakład Mikrobiologii Lekarskiej, Instytutu Mikrobiologii i Chorób Zakaźnych AM w Poznaniu.
Michalska
W
W
Drews
M
M
Marciniak
R
R
Lange
M
M
D003428:Cross Infection / Q000382:microbiology* / Q000635:transmission; D016172:DNA Fingerprinting; D005122:Exudates and Transudates / Q000382:microbiology*; D005838:Genotype; D006757:Hospital Units; D006801:Humans; D017757:Infectious Disease Transmission, Professional-to-Patient; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D009666:Nose / Q000382:microbiology; D009741:Nursing Staff, Hospital; D011044:Poland; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification* / Q000302:isolation & purification