An RNA fraction in chromatin of L-929 cells associated with DNA.
The DNA of L-929 cell nuclei was examined for the presence of an RNA fraction hybridized to the DNA that could possibly serve a role in gene regulation. A low molecular weight ([unk]4.5S) RNA fraction was observed as an RNA-DNA complex in chromatin preparations subfractionated on a Cs(2)SO(4) density gradient. This RNA does not appear to be covalently attached to the DNA. Neither does it appear to be attached to the DNA via a protein component. The RNA is resistant to ribonuclease H but is slowly attached by ribonuclease A + Tl. A role for, as well as the nature of the attachment of this RNA to DNA is suggested.
Monahan
J J
JJ
Hall
R H
RH
D000818:Animals; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D019286:Endopeptidase K; D006918:Hydroxyurea / Q000494:pharmacology; D007739:L Cells / Q000737:chemistry* / Q000187:drug effects; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization
D002843:Chromatin; D004247:DNA; D019286:Endopeptidase K; D006918:Hydroxyurea