title: Detection of intracytoplasmic cytokines by flow cytometry.
abstract: Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models.
authors.affiliation: Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil. santiago@gene.dbbm.fiocruz.br
authors.forename: M A
authors.initials: MA
authors.lastname: Santiago
authors.forename: P M
authors.initials: PM
authors.lastname: Luca
authors.forename: A L
authors.initials: AL
authors.lastname: Bertho
authors.forename: R B
authors.initials: RB
authors.lastname: Azeredo-Coutinho
authors.forename: S G
authors.initials: SG
authors.lastname: Coutinho
mesh_terms: D000818:Animals; D016207:Cytokines / Q000032:analysis*; D003593:Cytoplasm / Q000737:chemistry*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007892:Leishmania braziliensis; D016773:Leishmaniasis, Cutaneous / Q000276:immunology
chemical_list: D016207:Cytokines