Intercellular communication and cell proliferation in precision-cut rat liver slices: effect of medium composition and DDT.
Gap junctional intercellular communication (GJIC) and cell proliferation were studied in control and 1,1'-bis(p-chlorophenyl)-2, 2,2,-trichloroethane (DDT) treated precision-cut liver slices of rat by evaluating connexin 32 (Cx32) expression and 5-bromo-2'-deoxyuridine (BrdU) incorporation. In addition, the effect of different culture media (RPMI and WME) on control and DDT influenced Cx32 expression and cell proliferation was determined. Cx32 expression in control precision-cut liver slices was maintained during 8 h of culturing, but decreased after prolonged culturing. Control cell proliferation was significantly higher when WME was used as culture medium than when RPMI was used. In slices treated with DDT Cx32 expression was decreased. In slices cultured in RPMI medium, this decrease preceded a dose-dependent increase in cell proliferation. These results show the usefulness of precision-cut liver slices in studying cellular proliferation and intercellular communication.
de Graaf
I A
IA
TNO Nutrition and Food Research Institute, Toxicology Division, Zeist, Netherlands.
Tajima
O
O
Groten
J P
JP
Wolterbeek
A P
AP
D000818:Animals; D015153:Blotting, Western; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D017630:Connexins / Q000378:metabolism; D003470:Culture Media; D003634:DDT / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D017629:Gap Junctions / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D000097002:Gap Junction beta-1 Protein
D001973:Bromodeoxyuridine; D017630:Connexins; D003470:Culture Media; D003634:DDT; D007770:L-Lactate Dehydrogenase; D000097002:Gap Junction beta-1 Protein