Clonal analysis of the human B-cell repertoire using a heteromobility assay.
A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.
Tewari
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Aaron Diamond AIDS Center, New York, NY 10016, USA. dtewari@state.pa.us
D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis / Q000235:genetics*; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D016130:Immunophenotyping / Q000379:methods; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic / Q000276:immunology*
D000944:Antigens, Differentiation, B-Lymphocyte; D007135:Immunoglobulin Variable Region