title: Transforming growth factor-beta and interleukin-10 subvert alloreactive delayed type hypersensitivity in cardiac allograft acceptor mice.
abstract: We have previously reported that temporary treatment of cardiac allograft recipients with gallium nitrate (GN) results in indefinite graft survival, and the inability to mount donor-reactive delayed type hypersensitivity (DTH) responses. We report that antibodies to either transforming growth factor-beta (TGFbeta) or interleukin-10 (IL10) can uncover DTH responses to donor alloantigens in cardiac allograft acceptor mice. The DTH responses uncovered with TGFbeta-reactive antibodies can be blocked by exogenous IL10, and those uncovered with IL10-reactive antibodies can be blocked by exogenous TGFbeta. These data demonstrate that allograft acceptor mice are fully allosensitized, and poised to make donor-reactive cell-mediated immune responses. However, such responses are subverted by a donor alloantigen-dependent mechanism that involves TGFbeta and IL10, which in turn interfere with local cell-mediated immune responses.
authors.affiliation: Department of Surgery, The Ohio State University College of Medicine, Columbus 43210, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Bickerstaff
authors.forename: A M
authors.initials: AM
authors.lastname: VanBuskirk
authors.forename: E
authors.initials: E
authors.lastname: Wakely
authors.forename: C G
authors.initials: CG
authors.lastname: Orosz
mesh_terms: D000818:Animals; D000917:Antibody Formation; D016027:Heart Transplantation / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular / Q000502:physiology; D016753:Interleukin-10 / Q000276:immunology* / Q000494:pharmacology; D007518:Isoantibodies / Q000187:drug effects / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016212:Transforming Growth Factor beta / Q000276:immunology* / Q000494:pharmacology; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D007518:Isoantibodies; D007519:Isoantigens; D016212:Transforming Growth Factor beta; D016753:Interleukin-10