Ultrastructural changes in hypothermically preserved hepatocytes.
Hypothermic preservation of hepatocytes on gelatin gels (10 degrees C) provided a stable system for investigating cold-induced changes culminating in cell death. Hepatocyte morphology remained unchanged during 9 days of preservation. Thereafter there was a progressive movement of organelles toward the center of the cell. During this process the mitochondria appeared to have a normal morphology suggesting that they are not the primary cause of the transition. When the movement was completed the mitochondria appeared aggregated and microvilli were no longer apparent on the cell surface.
Griffiths
B J
BJ
Cardiff School of Biosciences, Cardiff University, Cardiff, CF1 3TL, United Kingdom.
Evans
P J
PJ
D015925:Cryopreservation; D005780:Gelatin; D005782:Gels; D008099:Liver / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000648:ultrastructure; D013997:Time Factors
D005782:Gels; D005780:Gelatin