title: Diagnosis of tuberculosis by a visually detectable immunoassay for lipoarabinomannan.
abstract: Recovery of tubercle bacilli from sputum, tissue, or body fluid is the standard for the diagnosis of tuberculosis (TB) although this process is technically demanding and relatively insensitive. We have developed a simplified, visually detectable, colloidal gold-based serological assay to qualitatively detect IgG directed against the mycobacterial cell wall component lipoarabinomannan (LAM). The objective of this investigation is to determine the accuracy of this assay in patients with active pulmonary TB and in control patients with or without latent infection. In patients with active TB, the sensitivity of anti-LAM IgG was 85 to 93%. In five patients with active TB who were smear-negative, all tested positive for anti-LAM IgG. The specificity of the test depended on the presence of tuberculous infection. In U.S. citizens comprised of young healthy adults and rheumatology patients, the specificity was 100%. In an at-risk population for tuberculous infection who were either tuberculin skin test-negative or positive, the specificity was 89%. The negative and positive predictive values of the test were 98% and 52%, respectively. We conclude that anti-LAM IgG immunoassay is relatively sensitive and specific for active TB and thus, a potentially useful screening test for active TB.
authors.affiliation: Division of Pulmonary Sciences and Critical Care Medicine, and Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, and National Jewish Medical and Research Center, Denver, Colorado, USA. chane@njc.org
authors.forename: E D
authors.initials: ED
authors.lastname: Chan
authors.forename: R
authors.initials: R
authors.lastname: Reves
authors.forename: J T
authors.initials: JT
authors.lastname: Belisle
authors.forename: P J
authors.initials: PJ
authors.lastname: Brennan
authors.forename: W E
authors.initials: WE
authors.lastname: Hahn
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007073:Immunoglobulin E / Q000097:blood*; D007194:India; D008070:Lipopolysaccharides / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011237:Predictive Value of Tests; D012307:Risk Factors; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*; D014481:United States
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; C050016:lipoarabinomannan; D007073:Immunoglobulin E