title: Human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50 are admixtures of the human colon carcinoma cell line HCT 116.
abstract: In two recently described human oesophageal adenocarcinoma cell lines JROECL 47 and JROECL 50, derived from one tumour, we detected identical E-cadherin and beta-catenin gene mutations as in colon carcinoma cell line HCT 116. We demonstrate by HLA-typing, mutation analysis and microsatellite analysis that cell lines JROECL 47 and JROECL 50 are admixtures of the human colon adenocarcinoma cell line HCT 116.
authors.affiliation: Erasmus University Medical Centre Rotterdam, Department of Surgery, University Hospital Dijkzigt, The Netherlands.
authors.forename: B P
authors.initials: BP
authors.lastname: Wijnhoven
authors.forename: M G
authors.initials: MG
authors.lastname: Tilanus
authors.forename: A G
authors.initials: AG
authors.lastname: Morris
authors.forename: S J
authors.initials: SJ
authors.lastname: Darnton
authors.forename: H W
authors.initials: HW
authors.lastname: Tilanus
authors.forename: W N
authors.initials: WN
authors.lastname: Dinjens
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D001483:Base Sequence; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm