Interferon gamma inhibits proliferation and hyaluronic acid adhesion of human malignant glioma cells in vitro.
Malignant gliomas are frequent and the prognosis is poor. The cytokine interferon gamma (IFN-gamma) enhances several immune phenomena and may be used in immunotherapy of tumours. Therefore we investigated the influence of IFN-gamma on human cell lines T98G, U87MG, 86HG39 and 85HG66, measuring cell viability (MTT-test) and proliferation (3H-thymidine uptake). IFN-gamma markedly decreased viability and proliferation of all investigated cell lines. Expression of CD44 and adhesion to hyaluronic acid (HA) are involved in glioma invasion. Influence of IFN-gamma on these two features has also been investigated. IFN-gamma markedly decreased HA-adhesion in all three investigated cell lines, whereas CD44 expression remained uninfluenced. To summarise, IFN-gamma strongly decreased cell growth and HA-adhesion of malignant glioma cell lines in vitro. We suggest further investigations to characterise better the role of IFN-gamma as a treatment opportunity for malignant gliomas.
Knüpfer
M M
MM
Department of Pediatric Hematology and Oncology, University of Leipzig, Germany. knuem@medizin.uni-leipzig.de
Knüpfer
H
H
Van Gool
S
S
Domula
M
M
Wolff
J E
JE
D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005910:Glioma; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D007553:Isotope Labeling; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured
D018960:Hyaluronan Receptors; D014316:Tritium; D007371:Interferon-gamma; D006820:Hyaluronic Acid; D013936:Thymidine