Human amniotic epithelial cells are promising transgene carriers for allogeneic cell transplantation into liver.
As human amniotic epithelial tissue is formed on about the eighth day after fertilization, human amniotic epithelial cells (hAEC) may have multipotency to differentiate into various organs, such as brain, heart, or liver. In this study, we showed evidence of the synthesis and excretion of albumin by hAEC, by immunostaining and enzyme-linked immunoassay. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot analyses revealed the expression of albumin mRNA and protein, respectively. In addition, hAEC also demonstrated immunoreactivity to genetic markers of liver lineage, such as human serum albumin and alpha-fetoprotein. Transplanted hAEC to Scid mouse liver showed positive immunoreactivity to albumin and alpha-fetoprotein. Genetically modified cells containing the beta-galactosidase (LacZ) gene (AxCALacZ) were integrated in liver parenchyma. Human polymorphic gene analysis in Scid mouse liver after the implantation of hAEC showed that these Scid mouse livers obviously contained this human-specific gene until day 7 after the cell transplantation. As hAEC do not cause any acute rejection by allotransplantation, we conclude that hAEC may be useful as a transgene carrier to treat patients with inherited liver diseases.
Sakuragawa
N
N
Department of Inherited Metabolic Disease, National Institute of Neuroscience, Kodaira, Tokyo, Japan. sakuraga@ncnp.go.jp
Enosawa
S
S
Ishii
T
T
Thangavel
R
R
Tashiro
T
T
Okuyama
T
T
Suzuki
S
S
D000418:Albumins / Q000096:biosynthesis; D000653:Amniotic Fluid / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D017690:Cell Transplantation; D004847:Epithelial Cells / Q000637:transplantation*; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000601:surgery*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019076:Transgenes; D014184:Transplantation, Homologous
D000418:Albumins