Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease.
A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.
Hansen
J N
JN
Pheiffer
B H
BH
Hough
C J
CJ
D000362:Agar; D001409:Bacillus cereus / Q000737:chemistry; D004269:DNA, Bacterial / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000302:isolation & purification*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D000362:Agar; D015719:Single-Strand Specific DNA and RNA Endonucleases