Prevalence of the carbapenemase gene (cfiA) among clinical and normal flora isolates of Bacteroides species in Hungary.
The carbapenemase gene (cfiA) was detected in 4 (5.7%) of 70 clinical isolates of Bacteroides fragilis from different parts of Hungary. Among 24 other Bacteroides species isolated from infectious processes or from normal faecal flora, none was cfiA-positive. The MIC of imipenem and meropenem for all cfiA-positive B. fragilis isolates was < or =0.25 mg/L, but 17% of the B. fragilis and 46% of the non-fragilis Bacteroides isolates exhibited reduced susceptibility to imipenem (MICs 0.5-2 mg/L). Only one of these isolates produced increased levels of beta-lactamase. No difference was observed in the outer-membrane proteins of B. fragilis isolates that harboured the cfiA gene and those with reduced susceptibility to imipenem.
Sóki
J
J
Department of Clinical Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Urban
E
E
Department of Clinical Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Szöke
I
I
Department of Clinical Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Fodor
E
E
Department of Clinical Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Nagy
E
E
Department of Clinical Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
D001426:Bacterial Proteins; D001439:Bacteroides / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D001442:Bacteroides Infections / Q000453:epidemiology / Q000382:microbiology; D015139:Blotting, Southern; D006801:Humans; D006814:Hungary / Q000453:epidemiology; D015378:Imipenem / Q000494:pharmacology; D000077731:Meropenem; D008667:Metalloproteins / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013845:Thienamycins / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; D008667:Metalloproteins; D013845:Thienamycins; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem