title: Proton magnetic resonance studies of ultraviolet-irradiated apurinic acid.
abstract: In apurinic acid, a single-stranded polydeoxyribonucleotide easily obtained upon depurination of DNA, the proton resonances arising from thymine and cytosine are readily observable in aqueous solution of 25 degrees C. Two methyl thymine resonances, centered at 1.88 ppm and separated by 0.045 ppm, are observed. We attribute the downfield methyl resonance to thymines with no pyrimidine nearest neighbors and the upfield methyl resonance to thymines having pyrimidine neighbors in the 3' and/or 5' positions. Upon ultraviolet irradiation, the upfield methyl and thymine H-6 resonances decrease in amplitude and two methyl resoances appear at 1.63 and 1.52 ppm, corresponding, respectively, to cytosine-thymine and thymine-thymine cyclobutane dimers. Photoreversal eliminates these two minor methyl resonances from the pmr spectrum. We conclude that apurinic acid provides a suitable model system for pmr studies of chemically modified pyrimidine bases in DNA.
authors.forename: R O
authors.initials: RO
authors.lastname: Rahn
authors.forename: T
authors.initials: T
authors.lastname: Schleich
mesh_terms: D001080:Apurinic Acid / Q000737:chemistry* / Q000528:radiation effects*; D003596:Cytosine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D011522:Protons; D013941:Thymine / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D011522:Protons; D001080:Apurinic Acid; D003596:Cytosine; D013941:Thymine