title: Efficient production of N-terminally truncated biologically active human interleukin-6 by Bacillus brevis.
abstract: cDNAs encoding human interleukin 6 (hIL-6) and its variants lacking the N-terminal Pro and Pro-Val-Pro-Pro, respectively, were expressed in Bacillus brevis by using the signal peptide fusion approach. The presence of Pro at the N-terminus of the mature protein hindered the action of the Bacillus brevis signal peptidase. hIL-6 lacking the N-terminal Pro-Val-Pro-Pro was most efficiently secreted in a biologically active form and accumulated in the culture medium to a level of 200 mg per liter, which is the highest level reported for the bacterial secretion of hIL-6.
authors.affiliation: School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shiga
authors.forename: M
authors.initials: M
authors.lastname: Maki
authors.forename: T
authors.initials: T
authors.lastname: Ohta
authors.forename: S
authors.initials: S
authors.lastname: Tokishita
authors.forename: A
authors.initials: A
authors.lastname: Okamoto
authors.forename: N
authors.initials: N
authors.lastname: Tsukagoshi
authors.forename: S
authors.initials: S
authors.lastname: Udaka
authors.forename: A
authors.initials: A
authors.lastname: Konishi
authors.forename: Y
authors.initials: Y
authors.lastname: Kodama
authors.forename: D
authors.initials: D
authors.lastname: Ejima
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: H
authors.initials: H
authors.lastname: Yamagata
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D001483:Base Sequence; D018076:DNA, Complementary; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D015850:Interleukin-6; D011994:Recombinant Proteins