Recognition of the nonpolar base 4-methylindole in DNA by the DNA repair adenine glycosylase MutY.
[formula: see text] The DNA repair adenine glycosylase MutY efficiently recognizes 7-deaza-2'-deoxyadenosine (Z) and its nonpolar isostere 4-methylindole beta-deoxynucleoside (M) opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) and G in DNA. Both wild-type and truncated MutY exhibit a 10- to 20-fold higher affinity for a duplex containing OG:M than OG:Z. More efficient recognition of M over Z by MutY may be to due the lack of hydrogen bonding with the OG that facilitates nucleotide flipping during the substrate recognition process.
Chepanoske
C L
CL
Department of Chemistry, University of Utah, Salt Lake City 84112, USA.
Langelier
C R
CR
Chmiel
N H
NH
David
S S
SS
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D045647:DNA Glycosylases; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D006151:Guanosine / Q000378:metabolism; D007211:Indoles / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000378:metabolism*; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides / Q000378:metabolism*
C099894:4-methylindole deoxynucleoside; D007211:Indoles; D009692:Nucleic Acid Heteroduplexes; D009705:Nucleosides; D006151:Guanosine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; C061738:mutY adenine glycosylase; D003849:Deoxyguanosine