Complete nucleotide sequence of the genomic RNA of a mild strain of Japanese yam mosaic potyvirus.
We determined the complete nucleotide sequence of a mild strain of Japanese yam mosaic potyvirus (JYMV-M) and compared it with the published sequence of severe strain of JYMV (JYMV-J1). The genomic RNA of JYMV-M is 9,760 nucleotides (nts) in length, excluding the poly (A) tail, and encodes a polyprotein of 3,132 amino acids. Among nine potential cleavage sites, only the P1 and NIa recognition sites (between 6K1 and CI) had different sequences from those of JYMV-J1. The data confirm the strain status of these two viruses with 91.1% sequence identity for the polyprotein and approximately 94-97% identities for HC, CI, NIa, NIb and CP. The most divergent products P1 and P3 had 62% and 90% sequence identities respectively.
Fuji
S
S
Aichi-Prefectural Agricultural Research Center, Nagakute, Japan.
Nakamae
H
H
D001483:Base Sequence; D018076:DNA, Complementary; D016679:Genome, Viral; D019668:Liliaceae / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012367:RNA, Viral