title: Detection of point mutations of BCL10 gene in hepatocellular carcinoma tissues: report of 46 cases.
abstract: BCL10 was found to have truncated mutations at a high frequency in MALT (mucosa-associated lymphoid tissue) B cell lymphomas. We examined the mutations of BCL10 gene in human primary liver cancer using non-isotopic PCR-SSCP. Three exons were examined in both cancer and non-HCC adjacent liver tissues. For each exon, six PCR products with abnormal bands were sequenced to verify those mutations. 56.5% samples were revealed a C to G mutation at position 5744 (g5744C>G) of the first exon of BCL10 gene; 54.3% samples were revealed a T deletion mutation at position 11311 (g11311delT) of the second exon of BCL10 gene; 45.7% samples were revealed a C to T mutation at position 14116 (g14116C>T) of the third exon of BCL10 gene. Similar mutation types were found in tumor-adjacent tissues at a lower frequency. The single base changes result in a truncated BCL10 protein expression. Serum alpha-fetoprotein (AFP) level, the tumor size had no significant relationship with BCL10 mutation.
authors.affiliation: Department of Hepatobiliary Surgery, People's Hospital, Beijing Medical University, Beijing, 100044, USA.
authors.forename: C
authors.initials: C
authors.lastname: Jihua
authors.forename: L
authors.initials: L
authors.lastname: Xisheng
authors.forename: C
authors.initials: C
authors.lastname: Shengli
authors.forename: C
authors.initials: C
authors.lastname: Zongxian
authors.forename: C
authors.initials: C
authors.lastname: Guang
authors.forename: D
authors.initials: D
authors.lastname: Ruyu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics* / Q000473:pathology; D005091:Exons / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000074018:B-Cell CLL-Lymphoma 10 Protein; C118591:BCL10 protein, human; D009363:Neoplasm Proteins; D000509:alpha-Fetoproteins