title: Effects of pHGF on hepatocyte DNA synthesis after partial hepatectomy in rats.
abstract: The effects of pHGF on the changes of hepatocyte proliferative cycle and liver regenerative capacity after partial hepatectomized rats were observed by flow cytometry (FCM). The results were as follows: 1) S phase fraction (SPF) in group of normal rats (group A) accounted for 9.89% and increased gradually within 6 h, following a peak at 12th h or 36th h after operation, but in the group of pHGF-treated rats (group B) the peak appeared at 24th h after operation; 2) Proliferation index (PI) of group A was 19.6% before partial hepatectomy, increased to 34.91% within 6 h and reached a peaks at 12th or 36th h after operation, and in group B the peak appeared at 48th h after operation. There were significant differences between two groups in SPF and PI (P < 0.01). The weight of liver began to increase 12 h after operation, and almost reached the preoperative weight 5 days after operation. These findings suggest that pHGF can promote the DNA synthesis and segmentation of hepatocyte.
authors.affiliation: Institute of Liver Diseases, Tongji Hospital, Tongji Medical University, Wuhan.
authors.forename: W
authors.initials: W
authors.lastname: Tang
authors.forename: K
authors.initials: K
authors.lastname: Liang
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Du
authors.forename: W
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D005434:Flow Cytometry; D006498:Hepatectomy / Q000379:methods; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D011498:Protein Precursors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011498:Protein Precursors; C086013:pro-hepatocyte growth factor; D017228:Hepatocyte Growth Factor; D004247:DNA