A rapid chromatographic method to separate the subunits of bacterial luciferase in urea-containing buffer.
Clark
A C
AC
Department of Biochemistry and Molecular Biophysics, North Carolina State University, Raleigh 27695-7622, USA.
Noland
B W
BW
Baldwin
T O
TO
D002021:Buffers; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D008156:Luciferases / Q000737:chemistry / Q000302:isolation & purification*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D014508:Urea
D002021:Buffers; D011994:Recombinant Proteins; D014508:Urea; D008156:Luciferases