title: Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury.
abstract: To assess the importance of endothelial intercellular adhesion molecule-1 (ICAM-1) in microvascular leukocyte kinetics in diseased lungs, we investigated the transitional changes in ICAM-1 expression, vascular diameter, and leukocyte behavior in rat pulmonary microcirculation during the development of acute lung injury (ALI) and chronic fibrosis (FIB) evoked by bleomycin (BLM). Observations were made in the isolated perfused lung with a real-time confocal laser luminescence microscope. Microvascular cell kinetics were evaluated by measuring the behavior of fluorescence- labeled leukocytes and erythrocytes in the presence or absence of anti-ICAM-1 monoclonal antibody (1A29). Arteriolar ICAM-1 showed little change at any time after BLM treatment. Venular ICAM-1 was first enhanced at the initial phase of ALI followed by the second upregulation at the early phase of FIB. Capillary ICAM-1 showed a sustained increase at both ALI and FIB. Arteriolar and venular diameters were not altered but capillary diameter decreased during ALI and early FIB stages. Although firm adherence of leukocytes to arteriolar and venular walls was not observed, rolling leukocytes were increased in venules both at the initial phase of ALI and at the early phase of FIB. The leukocyte rolling in venules correlated well with transitional changes in ICAM-1 and was inhibited by 1A29. Sustained entrapment of leukocytes in capillaries was attributed to changes in vascular diameter as well as augmented ICAM-1. In conclusion, ICAM-1 plays an important role in microvascular leukocyte recruitment in both ALI and FIB in the BLM-injured lung.
authors.affiliation: Departments of Medicine and Biochemistry, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Nishio
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Naoki
authors.forename: K
authors.initials: K
authors.lastname: Takeshita
authors.forename: H
authors.initials: H
authors.lastname: Kudo
authors.forename: N
authors.initials: N
authors.lastname: Miyao
authors.forename: H
authors.initials: H
authors.lastname: Tsumura
authors.forename: H
authors.initials: H
authors.lastname: Serizawa
authors.forename: M
authors.initials: M
authors.lastname: Suematsu
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001160:Arterioles / Q000473:pathology; D001761:Bleomycin; D002196:Capillaries / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000502:physiology*; D007962:Leukocytes / Q000473:pathology* / Q000502:physiology; D008168:Lung / Q000098:blood supply* / Q000473:pathology; D008297:Male; D008833:Microcirculation / Q000737:chemistry / Q000473:pathology; D018613:Microscopy, Confocal; D011652:Pulmonary Circulation; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000503:physiopathology; D014699:Venules / Q000473:pathology
chemical_list: D001761:Bleomycin; D018799:Intercellular Adhesion Molecule-1