title: Expressions of putative neurotransmitters and neuronal growth related genes in Merkel cell-neurite complexes of the rats.
abstract: Reverse transcription-polymerase chain reaction using 32P-labeled dCTP with specific primers for putative neurotransmitters related and neuronal growth-related genes (GAP-43, NGF and BDNF) were used to search for evidence of such substances in the Merkel cell. Merkel cell samples were made from sinus hairs in the facial skin of rats. The relative amount of mRNA in a tissue sample concentrated in Merkel cells was compared semiquantitatively to that from nearby tissue without Merkel cells. mRNAs for VIP, tyrosine hydroxylase, substance P were found in higher concentration in Merkel cells than in control tissues. mRNA for genes encoding pro-enkephalin, GAP-43, CGRP, NGF and BDNF were detected in the Merkel cell samples at concentration statistically equivalent to those found in the control tissues. It was concluded that the relative concentration of mRNAs for VIP, tyrosine hydroxylase and substance P is consistent with the possibility that Merkel cell acts as a possible transduction element in mechanical excitation of sense organs in which Merkel cells are present.
authors.affiliation: Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT. msleung@cuhk.edu.hk
authors.forename: M S
authors.initials: MS
authors.lastname: Leung
authors.forename: C C
authors.initials: CC
authors.lastname: Wong
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics; D019922:GAP-43 Protein / Q000235:genetics; D008297:Male; D018613:Microscopy, Confocal; D020932:Nerve Growth Factor / Q000235:genetics; D016501:Neurites / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology*; D014660:Vasoactive Intestinal Peptide / Q000235:genetics
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D019922:GAP-43 Protein; D018377:Neurotransmitter Agents; D014660:Vasoactive Intestinal Peptide; D020932:Nerve Growth Factor; D015740:Calcitonin Gene-Related Peptide