title: Inhibitory effect of Cordyceps sinensis and Cordyceps militaris on human glomerular mesangial cell proliferation induced by native LDL.
abstract: Native LDL, in low concentrations, promotes proliferation of cultured human glomerular mesangial cells. LDL stimulated [(3)H]-thymidine incorporation into DNA of human glomerular mesangial cells. Increased concentrations of LDL led to increased [(3)H]-thymidine incorporation. When LDL concentrations were 5, 10 and 50 microg ml(-1), [(3)H]-thymidine incorporation was 919.5+/-216, 1106+/-132, and 1200+/-210, respectively. When Cordyceps sinensis 100, 200, 300, 400 microg ml(-1) plus LDL 10 microg ml(-1) were added, [(3)H]-thymidine incorporation was 99+/-19 and 53+/-8, respectively, P<0.01 compared with controls. With Cordyceps militaris at similar concentrations plus LDL 10 microg ml(-1), [(3)H]-thymidine incorporation was respectively 192+/-75, 168+/-66, 145+/-53 and 72+/-16, P<0.01 compared with controls. The data suggest that LDL may play a critical role in mediating mesangial cell hypertrophy or proliferation involved in the development of glomerulosclerosis. Cordyceps sinensis and Cordyceps militaris inhibited, to a certain degree, proliferation of cultured human glomerular mesangial cell induced by LDL.
authors.affiliation: Department of Internal Medicine, Zhong Shan Hospital, Shanghai Medical University, P.R. China.
authors.forename: W
authors.initials: W
authors.lastname: Zhao-Long
authors.forename: W
authors.initials: W
authors.lastname: Xiao-Xia
authors.forename: C
authors.initials: C
authors.lastname: Wei-Ying
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002967:Claviceps / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005920:Glomerular Mesangium / Q000166:cytology*; D005923:Glomerulosclerosis, Focal Segmental / Q000382:microbiology; D006801:Humans; D006984:Hypertrophy; D008077:Lipoproteins, LDL / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D004247:DNA