title: Effect of age and menopause on serum concentrations of pentosidine, an advanced glycation end product.
abstract: BACKGROUND
  Pentosidine is an advanced glycation end product. Our aim is to investigate (a) the age-related change of serum pentosidine and (b) the effect of menopause on serum pentosidine.
  
  
  METHODS
  Using the high-performance liquid-chromatography method with column switching, we measured serum pentosidine in 140 healthy women aged 20-93 years. Serum creatinine was also measured. The samples of 13 young and 13 old subjects were used for the measurements of free pentosidine and fractions of pentosidine. Free pentosidine was measured without hydrolysis, and the fractions were measured with a 10,000 mol wt cutoff filter. To investigate the effect of menopause on pentosidine, two biochemical markers for bone turnover (CTx and osteocalcin) were measured in age-matched premenopausal and postmenopausal women (16 in each group).
  
  
  RESULTS
  Serum pentosidine significantly increased with age (r = .702, p < .0001 ). The values of serum pentosidine for the groups beyond the age of 50 were significantly higher than those for the younger groups. The value for the group aged 80-93 years was three times higher than that for the group aged 20-29 years. Serum pentosidine moderately and significantly correlated to serum creatinine (r = .483, p = .0001). Free pentosidine was detected in only 3 of 13 young subjects and 2 of 13 old subjects. The ratio of free to total pentosidine was 2.9% and 1.2% in young and old subjects, respectively. Pentosidine <10,000 mol wt was not detected in all subjects. Pentosidine >10,000 mol wt was detected in all subjects. Serum CTx and osteocalcin significantly increased in postmenopausal women compared with those of pre-menopausal women. There was no significant change in serum pentosidine between the premenopause group and the postmenopause group.
  
  
  CONCLUSIONS
  Serum pentosidine significantly increased with age in healthy subjects aged 20-93 years and correlated to serum creatinine. The changes of fractions of pentosidine with aging were not observed. There was no effect of menopause on pentosidine.
authors.affiliation: Department of Orthopaedic Surgery, Hamamatsu University School of Medicine, Japan. taka1m@hama-med.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Oikawa
authors.forename: A
authors.initials: A
authors.lastname: Nagano
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D015415:Biomarkers; D016723:Bone Remodeling; D001842:Bone and Bones / Q000378:metabolism; D003094:Collagen / Q000032:analysis; D003432:Cross-Linking Reagents / Q000378:metabolism*; D005260:Female; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000097:blood; D008593:Menopause / Q000502:physiology*; D008875:Middle Aged; D015675:Osteocalcin / Q000032:analysis
chemical_list: D015415:Biomarkers; D003432:Cross-Linking Reagents; D017127:Glycation End Products, Advanced; D015675:Osteocalcin; D003094:Collagen; D001120:Arginine; C062187:pentosidine; D008239:Lysine