Use of Agrobacterium expressing green fluorescent protein to evaluate colonization of sonication-assisted Agrobacterium-mediated transformation-treated soybean cotyledons.
Colonization and infection of soybean cotyledons by Agrobacterium tumefaciens and subsequent elimination of bacteria from cotyledons were monitored using bacteria expressing green fluorescent protein (GFP). GFP provided a quick, non-destructive method to evaluate, in real time, Agrobacterium colonization of cotyledon surfaces as well as infection of internal cells. GFP was first detected 7 h following inoculation of the cotyledon. By 36 h, GFP expression was very intense, and was limited to the adaxial surface of the cotyledon. Expression of GFP also served as a useful indicator of successful elimination of the bacterium from plant tissue following antibiotic treatment.
Finer
K R
KR
Department of Biological Sciences, Kent State University/Stark Campus, Canton and Department of Horticulture and Crop Sciences, OARDC, The Ohio State University, Wooster, OH, USA. kfiner@stark.kent.edu
Finer
J J
JJ
D016960:Agrobacterium tumefaciens; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D008856:Microscopy, Fluorescence; D013025:Glycine max / Q000382:microbiology*; D013997:Time Factors
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins