Macrorestriction fragment profiles reveal genetic variation of Cowdria ruminantium isolates.
Macrorestriction profile analysis by pulsed-field gel electrophoresis (PFGE) was used to distinguish between seven isolates of Cowdria ruminantium from geographically different areas. Characteristic profiles were generated for each isolate by using the restriction endonucleases KspI, SalI, and SmaI with chromosomal sizes ranging between 1,546 and 1,692 kb. Statistical analysis of the macrorestriction profiles indicated that all the isolates were distinct from each other; these data contribute to a better understanding of the epidemiology of this pathogen and may be exploited for the identification of genotype-specific DNA probes.
de Villiers
E P
EP
Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa.
Brayton
K A
KA
Zweygarth
E
E
Allsopp
B A
BA
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D015342:DNA Probes; D016995:Ehrlichia ruminantium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D005869:Ghana / Q000453:epidemiology; D006357:Heartwater Disease / Q000175:diagnosis / Q000453:epidemiology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping / Q000379:methods; D012675:Senegal / Q000453:epidemiology; D013019:South Africa / Q000453:epidemiology
D015342:DNA Probes