Characterization of an anticryptococcal protein isolated from human serum.
Human serum at low concentrations inhibits the growth of Cryptococcus neoformans in vitro. Fractionation of serum yielded a purified inhibitory protein with a molecular mass of approximately 81.8 kDa, a pI of approximately 6.2, and an amino acid sequence that matched that of human transferrin. The inhibitory activity and that of apotransferrin and 5% human serum were reversed by 10 microM freshly prepared FeCl(3).
Sridhar
S
S
Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center, and California Institute for Medical Research, San Jose, California, USA.
Ahluwalia
M
M
Brummer
E
E
Stevens
D A
DA
D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001059:Apoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002712:Chlorides; D003455:Cryptococcus neoformans / Q000187:drug effects*; D005290:Ferric Compounds / Q000494:pharmacology; D006801:Humans; D007113:Immunity, Innate; D014168:Transferrin / Q000302:isolation & purification / Q000494:pharmacology*
D000935:Antifungal Agents; D001059:Apoproteins; D001798:Blood Proteins; D002712:Chlorides; D005290:Ferric Compounds; D014168:Transferrin; C028284:apotransferrin; C024555:ferric chloride