[A comparison of the immune response induced by DNA or by an inactivated vaccine against tick-borne encephalitis].
BALB/c mice were immunized with recombinant plasmid DNA pSVK3-ENS1 and pcDNAI-NS3 containing, respectively, genes E-NS1 and NS3 of tick-borne encephalitis (TBE) virus. Antibodies to TBE virus proteins were detected in the blood sera of the immunized animals by the method of the enzyme immunoassay. Though the titers of virus-specific antibodies in the sera of mice immunized with protein vaccines exceeded those registered after immunization with DNA vaccines, essential protective immunity was observed after the use of both vaccines.
Morozova
O V
OV
Institute of Bioorganic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia.
Popova
R V
RV
Maksimova
T G
TG
Mitrofanova
E E
EE
Bakhvalova
V N
VN
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004353:Drug Evaluation, Preclinical; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D004675:Encephalitis, Tick-Borne / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D007114:Immunization / Q000379:methods; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D006023:Glycoproteins; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines