Expression of alpha-calcitonin gene-related peptide in the enteric nervous system of rat small intestine.
We first detected alpha-calcitonin gene-related peptide (alpha-CGRP) precursor mRNA in the enteric nervous system (ENS) of rat small intestine by reverse transcriptase polymerase chain reaction (RT-PCR). The nucleotide sequence of the RT-PCR product was completely identical to that found in other organs. By in situ hybridization using digoxygenin-labeled alpha-CGRP precursor cRNA probe, we found that antisense probes detected a signal on nerve cell bodies of both submucosal and myenteric plexuses. Our findings indicate that the rat ENS participates in synthesis of alpha-CGRP precursor.
Doi
Y
Y
Department of Anatomy, School of Medicine, University of Occupational and Environmental Health, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu, Japan.
Peng
H
H
Kudo
H
H
Hamasaki
K
K
Fujimoto
S
S
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis* / Q000235:genetics*; D017615:Enteric Nervous System / Q000378:metabolism*; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D007583:Jejunum / Q000294:innervation* / Q000378:metabolism; D008297:Male; D012322:RNA Precursors / Q000096:biosynthesis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012322:RNA Precursors; D012333:RNA, Messenger; D015740:Calcitonin Gene-Related Peptide