title: Endothelium during microarterial graft procurement and transplantation.
abstract: Microarterial grafts are prone to mechanical endothelial injury that can have profound effects on the transplanted vessel, a factor neglected in studies on vascular changes post-transplantation. The aim of this study was to document the integrity of the endothelial lining after both procurement and transplantation of rat aortic grafts, using a minimal touch technique with and without the use of topical papaverine. It was found that procurement using a simple minimal touch technique preserved the endothelium and transplantation could be performed without endothelial injury. The appearance of "normal" endothelium varied with the degree of distension of the artery, suggesting a dynamic endothelial architecture to accommodate changes in the surface area of the artery during pulsation. These findings indicate that transplantation of an arterial segment without injury to the intima is possible and stress the importance of technical controls after both procurement and transplantation to prevent the use of injured grafts and misleading results.
authors.affiliation: University of Queensland, Department of Surgery, Princess Alexandra Hospital, Brisbane, Queensland, Australia.
authors.forename: D A
authors.initials: DA
authors.lastname: Neil
authors.forename: S V
authors.initials: SV
authors.lastname: Lynch
authors.forename: I R
authors.initials: IR
authors.lastname: Hardie
authors.forename: D J
authors.initials: DJ
authors.lastname: Effeney
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000637:transplantation*; D004730:Endothelium, Vascular / Q000473:pathology*; D008866:Microsurgery; D051381:Rats; D011917:Rats, Inbred Lew; D017208:Rats, Wistar; D020858:Tissue and Organ Harvesting