Membrane proteins PmpG and PmpH are major constituents of Chlamydia trachomatis L2 outer membrane complex.
The outer membrane complex of Chlamydia is involved in the initial adherence and ingestion of Chlamydia by the host cell. In order to identify novel proteins in the outer membrane of Chlamydia trachomatis L2, proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. By silver staining of the protein profile, a major protein doublet of 100-110 kDa was detected. In-gel tryptic digestion and matrix-assisted laser desorption/ionization mass spectrometry identified these proteins as the putative outer membrane proteins PmpG and PmpH.
Mygind
P H
PH
Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK-8000, Aarhus, Denmark. perm@biobase.dk
Christiansen
G
G
Roepstorff
P
P
Birkelund
S
S
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin
D001425:Bacterial Outer Membrane Proteins; D010446:Peptide Fragments; C410161:PmpG protein, Chlamydia trachomatis; C410162:PmpH protein, Chlamydia trachomatis; D014357:Trypsin