title: Scintigraphic detection of acute experimental endocarditis with the technetium-99m labelled glycoprotein IIb/IIIa receptor antagonist DMP444.
abstract: Bacterial endocarditis is an important clinical problem that may result in persistent bacteraemia and irreversible cardiac damage. Since endocarditis is characterized by aggregation of activated platelets, fibrin and bacteria, we studied DMP444, a technetium-99m labelled high-affinity antagonist of the GP IIb/IIIa receptor that is expressed on activated platelets. In seven Beagle dogs (11-15 kg), the left ventricle was catheterized via the right carotid artery. One hour later, 5x10(7) colony forming units of Staphylococcus aureus were injected intracardially. Half an hour later, the catheter was removed. Two extra dogs underwent a complete sham procedure. One day after the intervention, five infected and the two non-infected dogs were injected with 37 MBq/kg 99mTc-DMP444 and two infected dogs with 37 MBq/kg 99mTc-IgG (used as a non-specific control agent) and imaged up to 4 h after injection. Samples were obtained for tissue counting, microbiology and histology. From 1 to 2 h post injection onward, there was clear focal accumulation of DMP444 in the aortic valve region when endocarditis was present, and this accumulation increased with time. The non-infected and the 99mTc-IgG injected dogs showed only persisting blood pool activity without any focal abnormality. At 4 h post injection, the in vivo valve-to-blood pool ratios were 1.87+/-0.18 in endocarditis, 1.01+/-0.05 in non-infected controls and 1.09+/-0.02 in 99mTc-IgG injected dogs (P<0.05). It is concluded that targeting activated platelets with the 99mTc-labelled GP IIb/IIIa antagonist DMP444 allows a final diagnosis of experimental bacterial endocarditis within 4 h owing to high, specific and fast in vivo uptake.
authors.affiliation: Department of Nuclear Medicine, University Hospital Nijmegen, The Netherlands. w.oyen@nugen.azn.nl
authors.forename: W J
authors.initials: WJ
authors.lastname: Oyen
authors.forename: O C
authors.initials: OC
authors.lastname: Boerman
authors.forename: F M
authors.initials: FM
authors.lastname: Brouwers
authors.forename: J A
authors.initials: JA
authors.lastname: Barrett
authors.forename: F W
authors.initials: FW
authors.lastname: Verheugt
authors.forename: D J
authors.initials: DJ
authors.lastname: Ruiter
authors.forename: F H
authors.initials: FH
authors.lastname: Corstens
authors.forename: J W
authors.initials: JW
authors.lastname: van der Meer
mesh_terms: D000208:Acute Disease; D000818:Animals; D001021:Aortic Valve / Q000000981:diagnostic imaging; D004285:Dogs; D004697:Endocarditis, Bacterial / Q000000981:diagnostic imaging* / Q000473:pathology; D007074:Immunoglobulin G; D009842:Oligopeptides; D015609:Organotechnetium Compounds; D010974:Platelet Aggregation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D013667:Technetium
chemical_list: C411949:DMP 444; D007074:Immunoglobulin G; D009842:Oligopeptides; D015609:Organotechnetium Compounds; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019275:Radiopharmaceuticals; D013667:Technetium