title: A hyperglycemic peptide hormone from the Caribbean shrimp Penaeus (litopenaeus) schmitti.
abstract: From a crude extract of the sinus glands of the shrimp Penaeus (litopenaeus) schmitti a peptide with hyperglycemic activity in a homologous bioassay was isolated and characterized by a combination of automatic Edman degradation, enzymatic digestions, TLC of dansyl-amino acids, and mass spectrometry. Its M(r) is 8359.4 Da by MS, which coincides with the deduced sequence. Its N-terminus is free and its C-terminus is amidated. It has 6 Cys residues in conserved positions compared with other known CHHs. This is the first sinus gland hormone from an Atlantic Ocean shrimp characterized to date. It has a remarkable 90% sequence similarity to the Indo-Pacific shrimp P. (marsupenaeus) japonicus Pej-VII hyperglycemic hormone.
authors.affiliation: Instituto Nacional de la Nutrición 'Salvador Zubirán,' Department of Biochemistry, Vasco de Quiroga 15, Tlalpan, Mexico City, Mexico. amhuber@servidor.unam.mx
authors.forename: A
authors.initials: A
authors.lastname: Huberman
authors.forename: M B
authors.initials: MB
authors.lastname: Aguilar
authors.forename: I
authors.initials: I
authors.lastname: Navarro-Quiroga
authors.forename: L
authors.initials: L
authors.lastname: Ramos
authors.forename: I
authors.initials: I
authors.lastname: Fernández
authors.forename: F M
authors.initials: FM
authors.lastname: White
authors.forename: D F
authors.initials: DF
authors.lastname: Hunt
authors.forename: J
authors.initials: J
authors.lastname: Shabanowitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D004702:Endocrine Glands / Q000737:chemistry*; D010450:Endopeptidases; D005947:Glucose / Q000378:metabolism*; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D007447:Invertebrate Hormones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D033561:Penaeidae; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D007447:Invertebrate Hormones; D010450:Endopeptidases; D005947:Glucose