Analysis of Vibrio mimicus clinical strains by arbitrarily primed polymerase chain reaction.
A total of 51 Vibrio mimicus clinical strains from different geographic locations were examined by arbitrarily primed polymerase chain reaction (AP-PCR). The primer VMH-3 divided them into 28 groups, although 18 groups consisted of a single strain at present. All groups had a common 1.0-kb amplification fragment. Most of the groups consisted of strains from same region, although two exceptional groups showed a few amplification fragments including strains from different regions. AP-PCR groups were not consistently associated with serogroups. AP-PCR is thought to be a valuable and easy method for the epidemiological study of V. mimicus.
Bi
K
K
Faculty of Pharmaceutical Sciences, Okayama University, Japan. bikw@pheasant.pharm.okayama-u.ac.jp
Shi
L
L
Maehara
Y
Y
Miyoshi
S
S
Tomochika
K
K
Shinoda
S
S
D017931:DNA Primers; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014733:Vibrio / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D014735:Vibrio Infections / Q000453:epidemiology / Q000382:microbiology*
D017931:DNA Primers