Molecular epidemiological studies on foot-and-mouth disease type O Taiwan viruses from the 1997 epidemic.
Sequence diversity was assessed of the complete VP1 gene directly amplified from 49 clinical specimens during an explosive foot-and-mouth disease (FMD) outbreak in Taiwan. Type O Taiwan FMD viruses are genetically highly homogenous, as seen by the minute divergence of 0.2-0.9% revealed in 20 variants. The O/HCP-0314/TW/97 and O/TCP-022/TW/97 viral variants dominated FMD outbreaks and were prevalent in most affected pig-raising areas. Comparison of deduced amino acid sequences around the main neutralizable antigenic sites on the VP1 polypeptide showed no significant antigenic variation. However, the O/CHP-158/TW/97 variant had an alternative critical residue at position 43 in antigenic site 3, which may be due to selective pressure in the field. Two vaccine production strains (O1/Manisa/Turkey/69 and O1/Campos/Brazil/71) probably provide partial heterologous protection of swine against O Taiwan viruses. The type O Taiwan variants clustered in sublineage A1 of four main lineages in the phylogenetic tree. The O/Hong Kong/9/94 and O/1685/Moscow/Russia/95 viruses in sublineage A2 are closely related to the O Taiwan variants. The causative agent for the 1997 epidemic presumably originated from a single common source of type O FMD viruses prevalent in neighboring areas.
Tsai
C P
CP
Department of Pathobiology, Pig Research Institute Taiwan, P.O. Box 23, Chunan, Miaoli, Taiwan.
Pan
C H
CH
Liu
M Y
MY
Lin
Y L
YL
Chen
C M
CM
Huang
T S
TS
Cheng
I C
IC
Jong
M H
MH
Yang
P C
PC
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D016384:Consensus Sequence; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D004196:Disease Outbreaks / Q000662:veterinary*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D000939:Epitopes / Q000737:chemistry; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology*; D014644:Genetic Variation / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D013624:Taiwan / Q000453:epidemiology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D017931:DNA Primers; D004279:DNA, Viral; D000939:Epitopes; D012367:RNA, Viral; D014764:Viral Proteins