title: Regulation of activator protein-1-DNA binding activity by opioid peptides in estrogen-sensitive cells of rat hypothalamus and uterus.
abstract: The present studies demonstrate, for the first time, that the binding of activator protein-1 (AP-1)-DNA in rat uterus and the estrogen-sensitive areas of the hypothalamus, as measured by electrophoretic mobility shift assay, is increased 2 h after intraperitoneal injection of [D-Met(2),Pro(5)]enkephalinamide. The effect was prevented by the opiate antagonist naltrexone given 30 min before the administration of [D-Met(2),Pro(5)]enkephalinamide, suggesting the involvement of opioid peptide receptors in the observed effects. The present findings support the role of opioid peptides in the regulation of transcription in estrogen-sensitive cells.
authors.affiliation: Institute of Physiology, University Medical School of Pécs, Pécs, Hungary. oszter@physiol.pote.hu
authors.forename: A
authors.initials: A
authors.lastname: Oszter
authors.forename: B
authors.initials: B
authors.lastname: Törocsik
authors.forename: Z
authors.initials: Z
authors.lastname: Vértes
authors.forename: J L
authors.initials: JL
authors.lastname: Környei
authors.forename: K A
authors.initials: KA
authors.lastname: Kovács
authors.forename: M
authors.initials: M
authors.lastname: Vértes
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D018847:Opioid Peptides / Q000494:pharmacology*; D051381:Rats; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D004967:Estrogens; D018847:Opioid Peptides; D018808:Transcription Factor AP-1; D004247:DNA