title: Survival and recovery of viable but noncultivable forms of Campylobacter in aqueous microcosm.
abstract: Previous studies suggesting that the persistence of thermotolerant Campylobacter in water, especially as a viable but non-cultivable form (VNC), was involved in human campylobacteriosis, the capacities of survival and resuscitation of a significant collection of 85 strains in aqueous microcosms were investigated. Two-thirds of these strains (68%) were not detectable on agar medium after a stay of 14-21 days, whereas 21% reached this state before 14 days and 11% were non-cultivable after a stay of 21 days. Some strains remained cultivable after 35 days in a shaken aqueous microcosm and beyond 60 days without shaking. After 30 days, 51% of the non-detectable strains by conventional culture were recovered after injection in 9-day fertilised chicken eggs. A kinetic study showed that the age of the viable but non-cultivable forms and characteristics of the strains could explain the variations of recovery. These results suggest that viable but non-cultivable forms of Campylobacter could be a potential risk of colonisation of human or animals and that an embryonic factor seems to be essential to allow resuscitation.
authors.affiliation: Institut Universitaire de Technologie de Saint Brieuc, Université de Rennes I, France.
authors.forename: R
authors.initials: R
authors.lastname: Talibart
authors.forename: M
authors.initials: M
authors.lastname: Denis
authors.forename: A
authors.initials: A
authors.lastname: Castillo
authors.forename: J M
authors.initials: JM
authors.lastname: Cappelier
authors.forename: G
authors.initials: G
authors.lastname: Ermel
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000254:growth & development*; D006801:Humans