Taurine-induced attenuation of MPP+ neurotoxicity in vitro: a possible role for the GABA(A) subclass of GABA receptors.
Taurine is a sulphur-containing beta-amino acid found in high (millimolar) concentrations in excitable tissues such as brain and heart. Its suggested roles include osmoregulator, thermoregulator, neuromodulator, and potential neurotransmitter. This amino acid has also been shown to be released in large concentrations during ischaemia and excitotoxin-induced neuronal damage. Here we report a protective effect of taurine against MPP(+)-induced neurotoxicity in coronal slices from rat brain. Significant protective effects were observed at taurine concentrations of 20 and 1 mM, suggesting a potential role for taurine in cases of neuronal insult. Studies with the synthetic taurine analogues taurine phosphonate, guanidinoethane sulphonate, and trimethyltaurine suggested the observed effect to be mediated via an extracellular mechanism. The use of GABA receptor ligands muscimol and bicuculline indicated the effect to be mediated through activation of GABA(A) receptors.
O'Byrne
M B
MB
Department of Biochemistry, Trinity College, Dublin, Ireland. Muriel_B_O'Byrne@sbphrd.com
Tipton
K F
KF
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D004338:Drug Combinations; D005260:Female; D018755:GABA Agonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D008024:Ligands; D009118:Muscimol / Q000494:pharmacology; D009498:Neurotoxins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000378:metabolism / Q000502:physiology*; D013654:Taurine / Q000031:analogs & derivatives / Q000494:pharmacology*
D004338:Drug Combinations; D018755:GABA Agonists; D018756:GABA Antagonists; D008024:Ligands; D009498:Neurotoxins; D011963:Receptors, GABA-A; D013654:Taurine; D009118:Muscimol; C009558:taurocyamine; D015655:1-Methyl-4-phenylpyridinium; D001640:Bicuculline