title: The inhibitory effects of IFN alpha-2b on EGFR expression and growth of cultured subconjunctival fibroblasts.
abstract: To investigate to what extent the growth of cultured subconjunctival fibroblasts was regulated by epidermal growth factor receptor (EGFR), we observed the expression of EGFR inhibited by interferon alpha-2b (IFN alpha-2b) and determined the growth of cultured subconjunctival fibroblasts by immunohistochemical and MTT methods. The linear relationships between the concentration of IFN alpha-2b and EGFR expression or growth were y = 1252.7-554.61 lgx (r = -0.98, P < 0.01), y = 0.523-0.0421 lgx (r = -0.93, P < 0.05) respectively. The concentration of IFN alpha-2b at doubled IC50 to EGFR (6.55 x 10(3) IU/ml) only inhibited the cell growth by 25.13%. The results suggested that the growth of subconjunctival fibroblasts may be controlled by many other growth factors because it was only partially inhibited by decreasing EGFR expression.
authors.affiliation: Department of Ophthalmology, Xiehe Hospital, Tongji Medical University, Wuhan.
authors.forename: X
authors.initials: X
authors.lastname: Xiong
authors.forename: H
authors.initials: H
authors.lastname: Wei
authors.forename: S
authors.initials: S
authors.lastname: Du
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology*; D066246:ErbB Receptors / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D011994:Recombinant Proteins
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D066246:ErbB Receptors