title: Pairing of oligosaccharides in the Fc region of immunoglobulin G.
abstract: The Fc portion of immunoglobulin G (IgG) expresses paired oligosaccharides with microheterogeneities, which are associated with efficiencies of effector functions and with pathological states. A comparison of electrospray ionization mass spectrometry data obtained using a variety of Fc fragments derived from human and mouse IgG that do and do not retain the inter-chain disulfide bridge(s) revealed that (1) the Fc portion can be asymmetric as well as symmetric with respect to glycosylation and (2) the ratios of the individual glycoforms are different from what is expected from the random pairing.
authors.affiliation: Suntory Institute for Bioorganic Research, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka, Japan. katsuyoshi_masuda@suntory.co.jp
authors.forename: K
authors.initials: K
authors.lastname: Masuda
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: I
authors.initials: I
authors.lastname: Shimada
authors.forename: Y
authors.initials: Y
authors.lastname: Arata
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002240:Carbohydrate Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry; D005260:Female; D006031:Glycosylation; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000737:chemistry* / Q000302:isolation & purification; D051379:Mice; D009439:Neuraminidase; D009844:Oligosaccharides / Q000737:chemistry*; D011247:Pregnancy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014470:Umbilical Cord
chemical_list: D000911:Antibodies, Monoclonal; D004220:Disulfides; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D009844:Oligosaccharides; D009439:Neuraminidase