title: The SOS induction of umu'-'lacZ fusion gene by oxidative damage is influenced by polyamines in Escherichia coli.
abstract: We report that polyamines have an effect on the SOS response of the umu operon in polyamine-deficient mutant and wild-type Escherichia coli strains carrying the umu'-'lacZ fusion. H2O2 effectively induces umu'-'lacZ in the wild type, but not significantly in the mutant strain. Exogenous polyamines did not restore the umu induction in the mutant to the wild-type level. In logarithmically growing cells, the basal expression of umu gene in the mutant is about five times higher than that of the wild type. The addition of polyamines to the growth medium markedly reduces the basal expression to the wild-type level. This reduction is due not to growth rate but to the polyamine itself. Our results suggest that polyamines are essentially involved in the SOS induction of the umu operon in E. coli.
authors.affiliation: Department of Radiation Biology, Korea Atomic Energy Research Institute, Yusong, Taejon.
authors.forename: T J
authors.initials: TJ
authors.lastname: Oh
authors.forename: I G
authors.initials: IG
authors.lastname: Kim
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon; D016296:Mutagenesis / Q000502:physiology; D009876:Operon / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013014:SOS Response, Genetics / Q000502:physiology*; D013095:Spermidine / Q000494:pharmacology; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D011073:Polyamines; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase; D013095:Spermidine; D011700:Putrescine