UV-ozone ashing of cells and tissues for spatially resolved trace element analysis.
UV/ozone ashing of thin tissue sections and cell cultures is a simple technique to enhance relative elemental concentrations, while maintaining their spatial location at the sub-micron level. This approach may enhance the capability of spatially resolved analysis techniques to detect the distribution of trace elements in biological matrices. We present results from light microscopy and x-ray spectromicroscopy studies of tissues and cells demonstrating that the micro-structure is very well conserved. We show the signal enhancement resulting from the removal of carbon, which allows otherwise undetectable gadolinium to be mapped in cancer tissue for a novel neutron capture therapy.
Gilbert
B
B
Istitut de Physique Appliquee, Ecole Polytechnique Federale, PH-Ecublens, CH-1015 Lausanne, Switzerland. bgilbert@dpmail.epfl.ch
Perfetti
L
L
Hansen
R
R
Mercanti
D
D
Casalbore
P
P
Andres
R
R
Perfetti
P
P
Margaritondo
G
G
De Stasio
G
G
D002244:Carbon / Q000032:analysis; D016543:Central Nervous System Neoplasms / Q000737:chemistry; D005682:Gadolinium / Q000032:analysis; D005909:Glioblastoma / Q000737:chemistry*; D006801:Humans; D008577:Meningeal Neoplasms / Q000737:chemistry; D008579:Meningioma / Q000737:chemistry*; D008854:Microscopy, Electron / Q000379:methods*; D010126:Ozone / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D014131:Trace Elements / Q000032:analysis*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D014131:Trace Elements; D010126:Ozone; D002244:Carbon; D005682:Gadolinium