cDNA cloning, tissue expression, and chromosome mapping of human homolog of SOX18.
The SRY (sex-determining region Y) gene encodes a transcription factor characterized by a DNA-binding motif termed the HMG (high mobility group) domain. The SOX (Sry-box) genes comprise a large family related by homology to the HMG-box region. We isolated a cDNA clone with an open reading frame encoding a putative protein of 384 amino acids, which shared 83% identity to the mouse Sox18 protein. Northern blot analysis revealed that a 1.9-kb band of human SOX18 messenger RNAs was predominantly expressed in heart, although weak signals were seen in brain, liver, testis, and leukocyte. By polymerase chain reaction (PCR)-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel, the gene was mapped to the chromosome 20q13.33 region.
Azuma
T
T
Helix Research Institute, Kisarazu, Chiba, Japan.
Seki
N
N
Yoshikawa
T
T
Saito
T
T
Masuho
Y
Y
Muramatsu
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D015870:Gene Expression; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D055760:SOXF Transcription Factors; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; D006609:High Mobility Group Proteins; C095473:SOX18 protein, human; D055760:SOXF Transcription Factors; C490653:Sox18 protein, mouse; D014157:Transcription Factors