title: Time-dependent impairment of mitochondrial function after storage and transplantation of rabbit kidneys.
abstract: BACKGROUND
  The mitochondrial respiratory chain is implicated as a major target of kidney damage after ischemia-reperfusion. This study measures changes in integrated mitochondrial function and in the activity of enzymes of the respiratory chain after cold storage and transplantation-reperfusion in vivo.
  
  
  METHODS
  Mitochondrial oxygen consumption and activities of respiratory chain enzymes and citrate synthase were measured in cortical mitochondria isolated from rabbit kidneys after 1-48 hr of cold ischemia with or without transplantation-reperfusion.
  
  
  RESULTS
  State 4 mitochondrial oxygen consumption was significantly increased after 48 hr of ischemia or 24-48 hr of ischemia with transplantation. Prolonged (24 or 48 hr) ischemic storage with and without transplantation caused a significant decrease in state 3 oxygen consumption, as did transplantation after 1, 24, and 48 hr of cold storage. Complex I and complex II-III activity decreased after 24 or 48 hr of ischemia, with transplantation having little additional effect. Complex IV activity was significantly decreased after 48 hr of ischemia, this decrease being exacerbated by transplantation-reperfusion. Complex V activity decreased significantly after 1 hr of ischemia and continued to decrease after 24-48 hr of ischemia. Transplantation after 1-24 hr (but not 48 hr) of ischemia resulted in partial recovery of complex V activity. Citrate synthase activity was decreased significantly only after 48 hr of ischemia and reperfusion, consistent with the loss of mitochondrial membrane integrity seen in electron micrographs of the transplanted 48-hr group.
  
  
  CONCLUSIONS
  These data suggest that individual rabbit kidney mitochondrial complexes have different susceptibilities to cold ischemic and reperfusion damage.
authors.affiliation: Department of Surgical Research, Northwick Park Institute for Medical Research, Northwick Park Hospital, Harrow, United Kingdom. i.sammut@ic.ac.uk
authors.forename: I A
authors.initials: IA
authors.lastname: Sammut
authors.forename: K
authors.initials: K
authors.lastname: Burton
authors.forename: E
authors.initials: E
authors.lastname: Balogun
authors.forename: P
authors.initials: P
authors.lastname: Sarathchandra
authors.forename: K J
authors.initials: KJ
authors.lastname: Brooks
authors.forename: T E
authors.initials: TE
authors.lastname: Bates
authors.forename: C J
authors.initials: CJ
authors.lastname: Green
mesh_terms: D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D015925:Cryopreservation; D004579:Electron Transport / Q000502:physiology; D004798:Enzymes / Q000378:metabolism; D007668:Kidney / Q000502:physiology* / Q000648:ultrastructure; D016030:Kidney Transplantation; D008297:Male; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D010101:Oxygen Consumption; D011184:Postoperative Period; D011817:Rabbits; D013997:Time Factors
chemical_list: D004798:Enzymes; D002950:Citrate (si)-Synthase