title: Novel and sensitive method for the detection of anandamide by the use of its dansyl derivative.
abstract: The dansyl ester of anandamide was prepared and showed intense fluorescence on silica gel thin-layer chromatography when viewed under long-wavelength ultraviolet light (detection limit, 15 fmol). A high-performance liquid chromatography method for the quantitation of anandamide was developed using a C18 column (250 x 4.6 mm) with gradient elution (1% acetic acid-methanol) and detection at 255 nm. The method was applied to the measurement of anandamide in media from cultured hepatocytes. Sample preparation involved extraction with a C18 cartridge, derivatization with dansyl chloride, thin-layer chromatography, and quantitation. The detection limit in hepatocyte media is 4.3 nmol at a signal-to-noise ratio of three.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655, USA.
authors.forename: B
authors.initials: B
authors.lastname: Yagen
authors.forename: S
authors.initials: S
authors.lastname: Burstein
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis* / Q000737:chemistry; D002121:Calcium Channel Blockers / Q000032:analysis / Q000737:chemistry; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D003619:Dansyl Compounds / Q000737:chemistry; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001095:Arachidonic Acids; D002121:Calcium Channel Blockers; D003619:Dansyl Compounds; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; C029038:dansyl chloride; C078814:anandamide