title: RP-LC method for the determination of cetirizine in serum.
abstract: The development and evaluation of HPLC method for quantifying cetirizine in human serum is described. The method involves liquid phase extraction of cetirizine in methylene chloride, adding diazepam as an internal standard, followed by separation on a reversed phase C18 Novapak column (150 x 3.9 nm; 4 microm), and employing a UV-detection set at 230 nm at ambient temperature. The mobile phase consists of a 13 mM phosphoric acid solution and acetonitrile (61:39 v/v) adjusted to pH 2.8 with 5 M NaOH. The assay is linear from 10 to 500 ng ml(-1) with a detection limit of 5 ng ml(-1) and a mean recovery of 96.5%. The applicability of this method in pharmacokinetic studies is evaluated.
authors.affiliation: Department of Applied Chemical Sciences, Faculty of Science, Jordan University of Science and Technology, Irbid. mzaater@usa.net
authors.forename: M F
authors.initials: MF
authors.lastname: Zaater
authors.forename: Y R
authors.initials: YR
authors.lastname: Tahboub
authors.forename: N M
authors.initials: NM
authors.lastname: Najib
mesh_terms: D017332:Cetirizine / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006634:Histamine H1 Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006634:Histamine H1 Antagonists; D017332:Cetirizine