IgE-reactivity to major Japanese cedar (Cryptomeria japonica) pollen allergens (Cry j 1 and Cry j 2) by ELISA in dogs with atopic dermatitis.
The present study investigated IgE-reactivity to two major Japanese cedar (Cryptomeria japonica, C. japonica) pollen allergens (Cry j 1 and Cry j 2) in dogs with atopic dermatitis by use of a fluorometric ELISA. The serum samples from 27 dogs that showed IgE-sensitivity to crude C. japonica pollen allergen by ELISA were tested for specific IgE to the two major allergens. All 27 dogs had anti-Cry j 1 IgE, and 10 (37%) had anti-Cry j 2 IgE. Inhibition of binding of dog specific IgE to crude C. japonica pollen allergen was carried out by addition of Cry j 1. When serum samples containing anti-Cry j 1 IgE but no anti-Cry j 2 IgE were incubated with Cry j 1, specific IgE binding to crude C. japonica pollen allergen was almost abolished. These findings suggest that Cry j 1 is a major allergen in dogs.
Masuda
K
K
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Tsujimoto
H
H
Fujiwara
S
S
Kurata
K
K
Hasegawa
A
A
Taniguchi
Y
Y
Yamashita
K
K
Yasueda
H
H
DeBoer
D J
DJ
de Weck
A L
AL
Sakaguchi
M
M
D000818:Animals; D001667:Binding, Competitive; D003429:Cross Reactions / Q000276:immunology; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology* / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000378:metabolism*; D008297:Male; D011058:Pollen / Q000276:immunology* / Q000378:metabolism; D012680:Sensitivity and Specificity; D014197:Trees / Q000276:immunology
D007073:Immunoglobulin E