title: [The laboratory diagnosis of an outbreak of hemorrhagic fever at Oblivskaya village, Rostov Province: proof of the etiological role of the Crimean-Congo hemorrhagic fever virus].
abstract: The results of the molecular biological detection of the etiologic agent of hemorrhagic fever in Rostov Province are presented. The role of the causative agents of Astrakhan rickettsial fever, hemorrhagic fever with the renal syndrome, Q fever, leptospirosis and listeriosis has been excluded by means of such immunochemical reactions as the direct and indirect immunofluorescent tests, the solid-phase immunoenzyme assay, the complement fixation test and the agglutination test. The relationship between the cases of hemorrhagic fever in the focus of the outbreak and Crimean-Congo hemorrhagic fever virus has been demonstrated due to the use of the polymerase chain reaction with preliminary reverse transcription.
authors.affiliation: Ministry of Health of the Russian Federation, Moscow.
authors.forename: G G
authors.initials: GG
authors.lastname: Onishchenko
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Lomov
authors.forename: V I
authors.initials: VI
authors.lastname: Markov
authors.forename: V A
authors.initials: VA
authors.lastname: Merkulov
authors.forename: A N
authors.initials: AN
authors.lastname: Mironov
authors.forename: I V
authors.initials: IV
authors.lastname: Borisevich
authors.forename: A A
authors.initials: AA
authors.lastname: Makhlaĭ
authors.forename: N T
authors.initials: NT
authors.lastname: Vasil'ev
authors.forename: S O
authors.initials: SO
authors.lastname: Vodop'ianov
authors.forename: N G
authors.initials: NG
authors.lastname: Tikhonov
authors.forename: T P
authors.initials: TP
authors.lastname: Pashanina
authors.forename: V V
authors.initials: VV
authors.lastname: Manankov
authors.forename: V P
authors.initials: VP
authors.lastname: Smelianskiĭ
authors.forename: S T
authors.initials: ST
authors.lastname: Savchenko
authors.forename: S E
authors.initials: SE
authors.lastname: Smirnova
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D001483:Base Sequence; D017931:DNA Primers; D003937:Diagnosis, Differential; D004196:Disease Outbreaks; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis* / Q000453:epidemiology / Q000209:etiology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012426:Russia / Q000453:epidemiology; D012698:Serologic Tests / Q000379:methods
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers