title: [Streptomyces sp. Z-11-6--a new producer of extracellular L-glutamate oxidase].
abstract: Glutamate oxidase activity was studied in 1254 Streptomyces strains isolated from the zonal soils of various regions of Russia and other countries. Seven strains proved to be producers of extracellular L-glutamate oxidase. The most active producer strain was identified, and the conditions of enzyme biosynthesis were optimized. A multistep-mutagenesis and selection procedure allowed a genetically stable strain, Streptomyces sp. Z-11-6, to be obtained, whose glutamate oxidase activity was 40 times higher than that of the original natural isolate.
authors.affiliation: Department of Microbiology, Biological Faculty, Moscow State University, Vorob'evy gory, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Sukhacheva
authors.forename: A I
authors.initials: AI
authors.lastname: Netrusov
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis* / Q000378:metabolism; D016296:Mutagenesis; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
chemical_list: D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase