Establishment of monoclonal antibodies specific for Bacillus subtilis DB9011.
Bacillus subtilis DB9011 is a strain with useful functions for agriculture. To establish a method for the discrimination of this strain from others, monoclonal antibodies (MAbs) were prepared. Although two established MAbs (MAb9B6 and MAb14D2) cross-react with some other Bacillus strains in ELISA, only B. subtilis DB9011 vegetative cells are recognized by both MAbs. MAb14D2 recognizes flagellin, a 34-kDa unit protein of flagella. The two MAbs established will provide powerful tools with which detailed analysis of this bacterial strain can be obtained under environmental conditions.
Asano
Y
Y
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University, Kiryu, Japan.
Akaishi
E
E
Tajima
K
K
Shinozawa
T
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001407:Bacillus / Q000276:immunology; D001412:Bacillus subtilis / Q000276:immunology*; D003429:Cross Reactions; D051379:Mice; D008807:Mice, Inbred BALB C
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal