[Expression of reactive oxygen-species related enzymes in endothelial cells stimulated with glycated lipoproteins].
Glycated lipoproteins, which elevate the blood in diabetic patients, cause direct attenuation of endothelial function. Increased glycation of apolipoproteins may play a trigger role in the accelerated development of atherosclerosis in the patient with diabetes. Here we assessed whether glycated lipoproteins affect on the endothelial function with particular emphasis on the role of reactive oxygen species in vitro. Incubation of human aortic endothelial cells(HAEC) with glycated LDL had little influence on the expression of antioxidant enzymes such as nitric oxide synthase(NOS), Cu2+Zn(2+)-superoxide dismutase (Cu2+Zn(2+)-SOD), catalase, and p22 phox in the cells. In contrast, exposure of glycated HDL induced a marked decrease of Cu2+Zn(2+)-SOD, catalase, and endothelial NOS as well as a slight increase of p22 phox in HAEC in term of both protein and mRNA expression, suggesting that increased formation of reactive oxygen species such as O2- and OH radical participate in the deterioration for the function of vascular endothelial cells in diabetic patients.
Sakairi
K
K
Kanto Biochemical Laboratories, Kohnosu.
Matsunaga
T
T
Nakajima
T
T
Koyama
I
I
Yamada
S
S
Hirose
H
H
Sato
M
M
Komoda
T
T
D001161:Arteriosclerosis / Q000209:etiology; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000209:etiology; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D008075:Lipoproteins, HDL / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism*
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; D002374:Catalase; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase