Expression of adenovirally delivered gene products in healing osseous tissues.
Gene therapy has moved from the promise of laboratory investigation to the reality of clinical practice in just the last decade. Various methods for delivery of genes to host cells have been developed and utilized both in vitro and in vivo. From the perspective of the plastic surgeon, gene therapy holds the promise to augment healing in clinical situations that remain difficult to treat, such as chronic wounds, osteoradionecrosis, or possibly to expedite current clinical practices, such as distraction osteogenesis. The authors chose to investigate the potential for gene therapy in osseous tissues using a replication-deficient adenovirus vector to deliver the marker transgene beta-galactosidase. An adenovirus vector is ideal for use in situations in which transgene expression is desired for only a relatively short period of time, such as wound and fracture healing. Utilizing a rat mandibular osteotomy model, they demonstrated that, using an adenoviral vector, foreign genes can be delivered in a simple fashion and can be expressed in a reliable manner within and around the osteotomy site for at least 10 days. Furthermore, there was no evidence of transfection of distant tissues associated with local application of the adenovirus vector. With this information, clinicians may now attempt to deliver osteogenic and angiogenic genes in a site-specific fashion to improve and expedite osseous healing.
Spector
J A
JA
The Institute of Reconstructive Plastic Surgery, and the Department of Surgery, New York University Medical Center, NY 10016, USA.
Mehrara
B J
BJ
Luchs
J S
JS
Greenwald
J A
JA
Fagenholz
P J
PJ
Saadeh
P B
PB
Steinbrech
D S
DS
Longaker
M T
MT
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D008297:Male; D008334:Mandible / Q000601:surgery; D010027:Osteotomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling / Q000379:methods; D014945:Wound Healing / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D001616:beta-Galactosidase