Apoptosis of mouse embryonic stem cells induced by single cell suspension.
Embryonic stem cells (ES cells) are pluripotential, and are therefore used to construct gene knock-out mice. We found that the apoptosis of mouse ES cells was induced when the cells were dispersed as single cells, whereas this process was suppressed when they proliferated in aggregates. The apoptosis of ES cells was repressed when the cells were cultured on feeders prepared from STO cells, a cell line established from embryonic fibroblasts. Culture supernatants from STO cells did not block the apoptosis of ES cells, which suggests that a direct interaction between ES cells and STO cells is required for the suppression of apoptosis. The viability of ES cells examined by the trypan blue exclusion test or by the MTT ((3-4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction assay decreased dramatically when the cells were dispersed in phosphate-buffered saline PBS. Cellular activity was restored by the addition of culture medium for ES cells. Glucose in the medium was found to be a major factor responsible for the restoration. Amino acids also restored the decrease in reduction of MTT. Suspension of the ES cells in PBS(-) caused leakage of the nucleosome into cytoplasm. Results indicate that the single cell suspension of ES cells leads to leakage of substrates for oxidative phosphorylation from the mitochondria, and that these cells finally become committed to apoptosis.
Koyanagi-Katsuta
R
R
Department of Medico-Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Akimitsu
N
N
Arimitsu
N
N
Hatano
T
T
Sekimizu
K
K
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002450:Cell Communication; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D003584:Cytological Techniques; D004492:Edetic Acid / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D051379:Mice; D009707:Nucleosomes / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D000596:Amino Acids; D003470:Culture Media; D009707:Nucleosomes; D004492:Edetic Acid; D014357:Trypsin; D005947:Glucose