title: Comparison between in vivo and in vitro chemokine production in Helicobacter pylori infection.
abstract: BACKGROUND
  Enhanced gastric mucosal chemokine activity has been demonstrated in patients with Helicobacter pylori infection. However, little is known about the mechanisms involved in this phenomenon.
  
  
  OBJECTIVE
  To examine whether in vivo chemokine activity is similar to in vitro response of gastric epithelial cells infected by H. pylori.
  
  
  METHODS
  Antral biopsy specimens were obtained from patients with H. pylori infection for organ culture, isolation of H. pylori and histological examination.
  
  
  RESULTS
  In organ cultures of mucosal tissues, the levels of interleukin-8 and growth-related gene product a were elevated in patients with peptic ulcer disease compared with those with erosive gastritis or endoscopically normal mucosa. However, there were no significant differences in in vitro cultures of MKN45 or KATO III cells that were infected with H. pylori isolated from these same patients. These in vivo and in vitro alpha-chemokine levels showed no significant association with the presence of cagA gene and CagA protein, ureB genotype, or binding capacity to MKN45 or KATO III cells in individual H. pylori isolates. In contrast, in vivo mucosal alpha-chemokine activity correlated with H. pylori colonization density.
  
  
  CONCLUSIONS
  Mucosal chemokine profiles and inflammatory responses in H. pylori infection may be associated more closely with host factors, including those determining bacterial adhesiveness, than with differences in H. pylori strains.
authors.affiliation: First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohsuga
authors.forename: K
authors.initials: K
authors.lastname: Kusugami
authors.forename: K
authors.initials: K
authors.lastname: Ina
authors.forename: T
authors.initials: T
authors.lastname: Ando
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: A
authors.initials: A
authors.lastname: Imada
authors.forename: Y
authors.initials: Y
authors.lastname: Nishio
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: T
authors.initials: T
authors.lastname: Tsuzuki
authors.forename: M
authors.initials: M
authors.lastname: Noshiro
authors.forename: T
authors.initials: T
authors.lastname: Konagaya
authors.forename: H
authors.initials: H
authors.lastname: Kaneko
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D002460:Cell Line; D018925:Chemokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000502:physiology; D005753:Gastric Mucosa / Q000378:metabolism; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori; D006801:Humans
chemical_list: D000942:Antigens, Bacterial; D018925:Chemokines