title: Effects of increased ambient pressure on colon cancer cell adhesion.
abstract: Forces such as strain modulate intestinal epithelial biology. Shear and pressure influence other cells. The effects of pressure on human colon cancer cells are poorly understood. Increasing ambient pressure for 30 min by 15 mm Hg over atmospheric stimulated adhesion to matrix proteins of four human colon cancer cell lines and primary cells from three human colon cancers, but not bovine aortic smooth-muscle cells. This effect was energy dependent and cation dependent (blocked by azide and chelation), accompanied by tyrosine phosphorylation of intracellular proteins including focal adhesion kinase, and blocked by tyrosine kinase inhibition (genistein, tyrphostin, and erbstatin) and a functional antibody to the beta1 integrin subunit. Although pressure stimulated adhesion even in a balanced salt solution, baseline and pressure-stimulated adhesion were each substantially diminished in the absence of serum. These data suggest that relatively low levels of increased pressure may stimulate malignant colonocyte adhesion by a cation-dependent beta1-integrin-mediated mechanism, perhaps via focal adhesion kinase-related tyrosine phosphorylation. In addition to elucidating another aspect of physical force regulation of colonocyte biology, these findings may be relevant to the effects of increased pressure engendered by colonic peristalsis, surgical manipulation, or laparoscopic surgery on colon cancer cell adhesion.
authors.affiliation: Department of Surgery, Yale University, New Haven, CT 06520-8062, USA. marc.basson@yale.edu
authors.forename: M D
authors.initials: MD
authors.lastname: Basson
authors.forename: C F
authors.initials: CF
authors.lastname: Yu
authors.forename: O
authors.initials: O
authors.lastname: Herden-Kirchoff
authors.forename: M
authors.initials: M
authors.lastname: Ellermeier
authors.forename: M A
authors.initials: MA
authors.lastname: Sanders
authors.forename: R C
authors.initials: RC
authors.lastname: Merrell
authors.forename: B E
authors.initials: BE
authors.lastname: Sumpio
mesh_terms: D000818:Animals; D001011:Aorta; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000503:physiopathology*; D016326:Extracellular Matrix Proteins / Q000502:physiology; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology; D011312:Pressure; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D016326:Extracellular Matrix Proteins; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; C496050:PTK2 protein, human