Proliferative involvement of ENX-1, a putative human polycomb group gene, in haematopoietic cells.
Homeobox genes have important roles in haematopoiesis and are regulated in an activated state by the trithorax group (trxG) of genes. In a repressed state, they are regulated by the Polycomb group (PcG) of genes. ENX-1, a putative human PcG gene product, interacts with the proto-oncogene product Vav. We report an investigation of the role of ENX-1 in human haematopoiesis. CD34+ cells mobilized to peripheral blood strongly expressed ENX-1. When stimulated to proliferate, both T and B lymphocytes rapidly up-regulated ENX-1. ENX-1 was expressed in all cell lines of the various lineages examined. When HL-60 cells were differentiated to mature granulocytes with all-trans retinoic acid, ENX-1 was down-regulated. Moreover, ENX-1 antisense oligodeoxynucleotide suppressed DNA synthesis in HL-60 cells. Our data indicate that ENX-1 is involved in the proliferation of both normal and malignant haematopoietic cells.
Fukuyama
T
T
Cancer Centre, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Otsuka
T
T
Shigematsu
H
H
Uchida
N
N
Arima
F
F
Ohno
Y
Y
Iwasaki
H
H
Fukuda
T
T
Niho
Y
Y
D000970:Antineoplastic Agents / Q000494:pharmacology; D001402:B-Lymphocytes / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004912:Erythrocytes / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006098:Granulocytes / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008533:Megakaryocytes / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D063146:Polycomb-Group Proteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013601:T-Lymphocytes / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
D000970:Antineoplastic Agents; C000715470:MAS1 protein, human; D016376:Oligonucleotides, Antisense; D063146:Polycomb-Group Proteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D012097:Repressor Proteins; D014212:Tretinoin; D004247:DNA