The novel G-protein coupled receptor SALPR shares sequence similarity with somatostatin and angiotensin receptors.
A cDNA encoding a novel G-protein coupled receptor (GPCR) was isolated from a human cerebral cortex cDNA library by low stringency hybridization screening. This putative seven-transmembrane domain receptor of 469 amino acids was designated SALPR (Somatostatin- and Angiotensin- Like Peptide Receptor). SALPR shares the highest amount of amino acid similarity with the somatostatin (35% with SSTR5) and angiotensin receptors (31% with AT1). Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the SALPR mRNA is predominantly expressed in human brain regions, particularly the substantia nigra and pituitary, although the mRNA can also be detected in the peripheral tissues, albeit at low levels. Chromosomal mapping by radiation hybrid analysis localized the human SALPR gene to the chromosome 5p15.1-5p14. Transient expression of SALPR in COS-1 cells did not produce any binding sites for somatostatin or angiotensin II, indicating the necessity for further study to discover its ligand and physiological significance.
Matsumoto
M
M
Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Tsukuba, Ibaraki, Japan. matsum_m@yamanouchi.co.jp
Kamohara
M
M
Sugimoto
T
T
Hidaka
K
K
Takasaki
J
J
Saito
T
T
Okada
M
M
Yamaguchi
T
T
Furuichi
K
K
D000595:Amino Acid Sequence; D000809:Angiotensins / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D019556:COS Cells; D002540:Cerebral Cortex / Q000378:metabolism; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D007457:Iodine Radioisotopes; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011869:Radioligand Assay; D011945:Receptors, Angiotensin / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D017481:Receptors, Somatostatin / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013004:Somatostatin / Q000378:metabolism; D014018:Tissue Distribution
D000809:Angiotensins; D018076:DNA, Complementary; D007457:Iodine Radioisotopes; D012333:RNA, Messenger; C410092:RXFP3 protein, human; D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D017481:Receptors, Somatostatin; D013004:Somatostatin; D019204:GTP-Binding Proteins