[The effect of different volume expanders on neutrophil granulocyte function in vitro].
BACKGROUND
The influence of kolloids on the immune system is not well documented. In this study we investigated the effects of gelatine, hydroxyethylstarch (HES), human albumine, and dextrane on neutrophil function and receptor expression by flow cytometry.


METHODS
Whole blood of healthy volunteers was incubated for 30 minutes with either gelatine, HES (6% and 10%), dextrane 40 and 60, or human albumin 20%. Phagocytic capacity was determined by uptake of fluorescein-isothiocyanate labeled bacteria, the conversion of dihydrorhodamine 123 into fluorescent rhodamine 123 was used for oxidative burst measurements. Expression of complement receptors CD 11b and CD35 was investigated using fluorescein-isothiocyanate labeled antibodies.


RESULTS
Incubation with gelatine significantly increased expression of complement receptors and oxidative burst. Dextranes and HES had no influence on neutrophil function. Human albumin reduced the oxidative burst, whereas CD 35 expression was increased.


CONCLUSIONS
The physiological significance of these changes in a range of 10% has to be clarified in further investigations.
Welters
I D
ID
Abteilung für Anaesthesiologie und Operative Intensivmedizin, Klinikum, Justus-Liebig-Universität Giessen.
Spangenberg
U
U
Menzebach
A
A
Engel
J
J
Menges
T
T
Langefeld
T W
TW
Hempelmann
G
G
D001419:Bacteria / Q000276:immunology; D003911:Dextrans / Q000494:pharmacology; D005434:Flow Cytometry; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D016177:Macrophage-1 Antigen / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D010952:Plasma Substitutes / Q000494:pharmacology*; D011951:Receptors, Complement / Q000187:drug effects; D017463:Receptors, Complement 3b / Q000276:immunology; D016897:Respiratory Burst / Q000187:drug effects; D012709:Serum Albumin / Q000494:pharmacology
D003911:Dextrans; D006895:Hydroxyethyl Starch Derivatives; D016177:Macrophage-1 Antigen; D010952:Plasma Substitutes; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D012709:Serum Albumin; D005780:Gelatin