title: Granulocyte-macrophage colony-stimulating factor and interleukin-5 concentrations in premature neonates with eosinophilia.
abstract: OBJECTIVE
  Eosinophilia is common among premature neonates, but little is known about the cytokines responsible for influencing its onset in neonates. In adults and transgenic mice, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-5 (IL-5) influence the development of eosinophilia. We sought to prospectively determine whether these cytokines correlated with the development of eosinophilia in premature infants.
  
  
  METHODS
  We measured the absolute eosinophil count (AEC) and serial serum concentrations of GM-CSF and IL-5 in premature neonates with eosinophilia.
  
  
  RESULTS
  Among 201 premature neonates, 21 had an eosinophil count of > 700/microliter (occurrence, 10.5%). Of these 21 neonates, 4 had mild (700/microliter to 999/microliter), 13 had moderate (1000/microliter to 2999/microliter), and 4 had severe (> 3000/microliter) eosinophilia. No differences in gestational age or age at onset of eosinophilia were observed between the groups, but the duration of eosinophilia was less with mild than with moderate or severe eosinophilia. A total of 20 of the 21 patients had an infection or necrotizing enterocolitis diagnosed within 48 hours of the onset of eosinophilia. In patients with mild and moderate eosinophilia, serial GM-CSF and IL-5 concentrations were below the lowest enzyme-linked immunosorbent assay standard. There was no correlation between AEC and GM-CSF or IL-5 concentration in these infants. However, in one patient with severe eosinophilia, two distinct elevations in IL-5 were noted (34.6 and 46.0 pg/ml); each peak occurred 7 to 8 days before a peak in eosinophil count.
  
  
  CONCLUSIONS
  Eosinophilia is relatively common, and in those neonates with the highest AECs, the duration of eosinophilia can last for > 6 weeks. The majority of cases of eosinophilia occurred in temporal proximity to an infectious illness or necrotizing enterocolitis. Serum concentrations of IL-5 were elevated in only one of our patients with severe eosinophilia.
authors.affiliation: Department of Pediatrics, University of Florida College of Medicine, Gainesville 32610-0296, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Calhoun
authors.forename: S E
authors.initials: SE
authors.lastname: Sullivan
authors.forename: M
authors.initials: M
authors.lastname: Lunøe
authors.forename: Y
authors.initials: Y
authors.lastname: Du
authors.forename: R D
authors.initials: RD
authors.lastname: Christensen
mesh_terms: D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia / Q000097:blood*; D004804:Eosinophils / Q000473:pathology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D007235:Infant, Premature, Diseases / Q000097:blood*; D015848:Interleukin-5 / Q000097:blood*; D007958:Leukocyte Count; D011379:Prognosis; D011446:Prospective Studies
chemical_list: D015415:Biomarkers; D015848:Interleukin-5; D016178:Granulocyte-Macrophage Colony-Stimulating Factor