title: Sequence and organization analyses of a Zygosaccharomyces rouxii DNA fragment containing the HIS3 gene.
abstract: The nucleotide sequence of a 3.4 kb fragment containing the HIS3 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The fragment was cloned from a Z. rouxii genomic DNA library by complementation of a Saccharomyces cerevisiae his3 mutant strain. The sequenced DNA fragment contained three open reading frames; the middle one (678 bp long, predicting a protein of 226 amino acids) shared a high degree of similarity with HIS3 genes of other yeast species. In the promoter region of the putative ZrHIS3 gene, a T(c) element required for constitutive transcription was found. The GenBank Accession No. of the sequenced DNA region is Y18561.
authors.affiliation: Department of Membrane Transport, Institute of Physiology CzAcadSci, 142 20 Prague 4, Czech Republic. sychrova@biomed.cas.cz
authors.forename: H
authors.initials: H
authors.lastname: Sychrova
authors.forename: V
authors.initials: V
authors.lastname: Braun
authors.forename: J L
authors.initials: JL
authors.lastname: Souciet
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics*; D015723:Gene Library; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase