Morphine treatment affects the regulation of high mobility group I-type chromosomal phosphoproteins in C6 glioma cells.
High mobility group (HMG) I-type chromosomal phosphoproteins HMG I/Y and HMG I-C were investigated following morphine treatment of C6BU-1 glioma. Cells were labelled with [32P]-orthophosphoric acid. Electrophoretic profiles and autoradiograms of the control cells revealed the presence of HMG I and HMG I-C proteins. HMG Y was not detected. Northern blot analysis showed a single HMG I/Y transcript. Treatment with morphine lowered the [32P]-incorporation in HMG I and HMGI-C proteins and the level of the HMG I/Y transcript. However, it did not change the protein ratios on the Coomassie stained gels. These results suggest that morphine may trigger independent reaction pathways affecting either transcription regulation and/or postsynthetic phosphorylation of the preexisting HMG I-type proteins. In addition, opposing changes in the postsynthetic phosphorylation of HMG 14 and histones H1AB were also noticed.
Chakarov
S
S
Dept. of Biochemistry, University of Sofia St. Kliment Ohridsky, Bulgaria.
Chakalova
L
L
Tencheva
Z
Z
Ganev
V
V
Angelova
A
A
D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D001932:Brain Neoplasms / Q000378:metabolism*; D002875:Chromosomes / Q000187:drug effects / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma / Q000378:metabolism*; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000494:pharmacology*; D009020:Morphine / Q000494:pharmacology*; D051381:Rats; D012394:Rosaniline Dyes; D014157:Transcription Factors / Q000494:pharmacology*
D000701:Analgesics, Opioid; D006609:High Mobility Group Proteins; D012394:Rosaniline Dyes; D014157:Transcription Factors; D025741:HMGA1a Protein; D009020:Morphine; C048139:Coomassie blue