title: Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease.
abstract: A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.
authors.forename: J N
authors.initials: JN
authors.lastname: Hansen
authors.forename: B H
authors.initials: BH
authors.lastname: Pheiffer
authors.forename: C J
authors.initials: CJ
authors.lastname: Hough
mesh_terms: D000362:Agar; D001409:Bacillus cereus / Q000737:chemistry; D004269:DNA, Bacterial / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000302:isolation & purification*; D015719:Single-Strand Specific DNA and RNA Endonucleases
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D000362:Agar; D015719:Single-Strand Specific DNA and RNA Endonucleases