Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks.
We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.
Carnevale
S
S
Departamento de Parasitología, Instituto Nacional de Microbiología "Dr. Carlos G. Malbrán," and Hospital Municipal de Infecciosas "Dr. Francisco J. Muñiz," Buenos Aires, Argentina. jorsil@overnet.com.ar
Velásquez
J N
JN
Labbé
J H
JH
Chertcoff
A
A
Cabrera
M G
MG
Rodríguez
M I
MI
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D003967:Diarrhea / Q000175:diagnosis / Q000469:parasitology; D004587:Electrophoresis, Agar Gel; D005243:Feces / Q000469:parasitology*; D005374:Filtration; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D016816:Microsporida / Q000235:genetics* / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000175:diagnosis* / Q000276:immunology; D010209:Paper; D016133:Polymerase Chain Reaction / Q000379:methods*
D016054:DNA, Protozoan; D005404:Fixatives; D005557:Formaldehyde