Characterization of humic substances by capillary electrophoresis.
Capillary electrophoresis was used for the separation of humic acid (HA) from peat, soil, and vermicompost. The electropherograms show the presence of at least three peaks eluted between 6 and 11 min for all HA. The best analysis resolution was obtained with the use of borate buffers at pH 8.9. The HA analyzed have structural and charge similarity, which increases the difficulty of separation. Therefore, the shape of the peaks is broad and the CE profiles of all HA are similar. It is reasonable to assume that the broad band in the three regions is due to the acidic groups that have a similar structure. By comparing the results obtained for HA extracted from soil, peat, vermicompost, and the commercial sample, HA from peat had the major carbon content.
Landgraf
M D
MD
Instituto de Química de São Carlos, Universidade de São Paulo, Brazil.
Javaroni
R de C
Rde C
Rezende
M O
MO
D019075:Electrophoresis, Capillary / Q000379:methods; D006812:Humic Substances / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D012987:Soil / Q000032:analysis
D006812:Humic Substances; D012987:Soil