title: [A family of shuttle vectors for lactobacilli and other gram-positive bacteria based on the plasmid pLF1311 replicon].
abstract: A set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in gram-positive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc.) was created. The vectors are based on the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM 1311 belonging to a family of the sigma-type pE194-like plasmids. The vectors can replicate in gram-positive bacteria and Escherichia coli. They are stable in many gram-positive bacteria, have small sizes, and allow the selection of recombinants on media with X-Gal. The vectors that contain the region of initiation of the conjugal transfer of plasmid RP4 belonging to the incompatibility group IncP alpha can be mobilized in a great number of bacteria using a helper plasmid from E. coli but not from gram-positive bacteria.
authors.affiliation: All-Russia Research Institute of the Physiology, Biochemistry, and Feed of Livestock, Borovsk, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Aleshin
authors.forename: E V
authors.initials: EV
authors.lastname: Semenova
authors.forename: B V
authors.initials: BV
authors.lastname: Tarakanov
authors.forename: V A
authors.initials: VA
authors.lastname: Livshits
mesh_terms: D004261:DNA Replication; D005822:Genetic Vectors; D006094:Gram-Positive Bacteria / Q000235:genetics*; D007778:Lactobacillus / Q000235:genetics*; D011995:Recombination, Genetic