Metabolism of 7-fluoro-6-(3,4,5,6-tetrahydrophthalimido)-4- (2-propynyl)-2H-1,4-benzoxazin-3(4H)-one (S-53482, flumioxazin) in the rat: II. Identification of reduced metabolites.
On single oral administration of (14)C-S-53482 [7-fluoro-6-(3,4,5, 6-tetrahydrophthalimido)-4-(2-propynyl)-2H-1,4-benzoxazin-3( 4H)-one, Flumioxazin] labeled at the 1- and 2-positions of tetrahydrophthaloyl group to rats at 1 (low dose) or 100 (high dose) mg/kg, the radiocarbon was almost completely eliminated within 7 days after administration in both groups with generally very low residual (14)C tissue levels. The predominant excretion route was via the feces. The major fecal and urinary metabolites involved reduction or sulfonic acid addition reactions at the 1,2-double bond of the 3,4,5,6-tetrahydrophthalimide moiety and hydroxylation of the cyclohexene or cyclohexane ring. One urinary and four fecal metabolites were identified using chromatographic techniques and spectroanalyses (NMR and MS). Three of five identified metabolites were unique forms, reduced at the 1,2-double bond of the 3,4,5, 6-tetrahydrophthalimide moiety. On the basis of the metabolites identified in this study, the metabolic pathways of S-53482 in rats are proposed. To specify tissues forming reduced metabolites, an in vitro study was conducted. Reduction was found to take place in red blood cells.
Tomigahara
Y
Y
Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Kasugade-Naka, Konohana-Ku, Osaka, Japan.
Onogi
M
M
Kaneko
H
H
Nakatsuka
I
I
Yamane
S
S
D000284:Administration, Oral; D000818:Animals; D048588:Benzoxazines; D002250:Carbon Radioisotopes; D005260:Female; D006540:Herbicides / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010078:Oxazines / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010084:Oxidation-Reduction; D010797:Phthalimides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011865:Radioisotope Dilution Technique; D051381:Rats; D017207:Rats, Sprague-Dawley
D048588:Benzoxazines; D002250:Carbon Radioisotopes; D006540:Herbicides; D010078:Oxazines; D010797:Phthalimides; C106487:flumioxazin