title: Developmental expression of OSP/claudin-11.
abstract: Oligodendrocyte-specific protein (OSP/claudin-11) is a major component of CNS myelin and has been recently added to the claudin family of tight junction proteins. In this study, the developmental expression of OSP/claudin-11 was determined using in situ hybridization, immunohistochemistry (IH), and Western blot analysis. OSP/claudin-11 mRNA was expressed in a bimodal fashion. During prenatal development, OSP/claudin-11 mRNA was abundant in developing meninges, in areas adjacent to cartilage, and in mesoderm. In postnatal animals, OSP/claudin-11 was expressed primarily in developing oligodendrocytes and to a lesser extent, in testes. Double-labeled IH using O2-A progenitor cells revealed that OSP/claudin-11 expression occurs from the early progenitor stage and continues in mature oligodendrocytes. Electron microscopic IH localized OSP/claudin-11 to laminar myelin in the adult CNS. Western blot analysis of OSP/claudin-11 in developing brain revealed the expression of two separate transcripts that were developmentally regulated. These data demonstrate that OSP/claudin-11 expression is highly regulated during development and, therefore, may play an important role in growth and differentiation of oligodendrocytes and other cells outside the CNS.
authors.affiliation: Department of Neurology, UCLA School of Medicine, Los Angeles, California 90024, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Bronstein
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Tiwari-Woodruff
authors.forename: H I
authors.initials: HI
authors.lastname: Kornblum
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000254:growth & development*; D001923:Brain Chemistry / Q000502:physiology*; D002454:Cell Differentiation; D057167:Claudins; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D009186:Myelin Sheath / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009836:Oligodendroglia / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D013234:Stem Cells / Q000502:physiology; D013737:Testis / Q000254:growth & development / Q000294:innervation / Q000378:metabolism
chemical_list: D057167:Claudins; C492353:Cldn11 protein, rat; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins