title: Autologous chondrocyte transplantation for reconstruction of isolated joint defects: the Assaf Harofeh experience.
abstract: BACKGROUND
  Articular cartilage is incapable of undergoing self-repair since chondrocytes lose their mitotic ability as early as the first year of life. Defects in articular cartilage, especially in weight-bearing joints, will predictably deteriorate toward osteoarthritis. No method has been found to prevent this deterioration. Drilling of the subchondral bone can lead to fibrocartilage formation and temporary repair that slowly degrades. Animal experiments indicate that introducing proliferating chondrocytes such as cultured articular chondrocytes can reliably reconstruct joint defects.
  
  
  OBJECTIVE
  To describe our clinical experience in culturing and transplanting autologous chondrocytes.
  
  
  METHODS
  Biopsies were obtained from 10 patients, aged 18-45, undergoing a routine arthroscopy in which a cartilage defect was identified with indications for cartilage transplantation. The biopsies were further processed to establish chondrocyte cultures. ACT was performed in 8 of the 10 patients because of persistent symptoms for at least 2 months post-arthroscopy. All patients (6 men and 2 women) had a grade IV cartilage defect in the medial or lateral femoral condyle, and three had a defect in the trochlear region as well. Biopsies were removed from the lateral rim of the superior aspect of the femur, and cells were cultured in a clean room. Following a 2 order of magnitude expansion, cells were implanted under a periosteal flap.
  
  
  RESULTS
  The eight patients implanted with autologous cells were followed for 6 months to 5 years (average 1 year). Complaints of giving-way, effusion and joint locking resolved in all patients, and pain as assessed by the visual analogue score was reduced by an average of 50%. Follow-up magnetic resonance imaging studies in all patients revealed that the defects were filled with tissue having similar signal characteristics to cartilage.
  
  
  CONCLUSIONS
  Chondrocyte implantation is a procedure capable of restoring normal articular cartilage in cases with isolated joint defects. Pain can be predictably reduced, while joint locking and effusion are eliminated. The effect on osteoarthritis progression in humans has not yet been elucidated.
authors.affiliation: Department of Orthopedics, Assaf Harofeh Medical Center, Zerifin, Israel. robinsn@netvision.net.il
authors.forename: D
authors.initials: D
authors.lastname: Robinson
authors.forename: H
authors.initials: H
authors.lastname: Ash
authors.forename: D
authors.initials: D
authors.lastname: Aviezer
authors.forename: G
authors.initials: G
authors.lastname: Agar
authors.forename: N
authors.initials: N
authors.lastname: Halperin
authors.forename: Z
authors.initials: Z
authors.lastname: Nevo
mesh_terms: D000293:Adolescent; D000328:Adult; D001182:Arthroscopy; D002357:Cartilage Diseases / Q000601:surgery*; D002358:Cartilage, Articular / Q000166:cytology / Q000293:injuries; D018929:Cell Culture Techniques / Q000379:methods; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007592:Joint Diseases / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D011182:Postoperative Care; D014182:Transplantation, Autologous; D016896:Treatment Outcome