title: Gene expression profiling of advanced lung cancer.
abstract: Lung cancer is a highly aggressive neoplasm with 85% mortality. To identify new tumor-associated genes, we compared the expression profile of a primary metastasizing adenocarcinoma with normal airway epithelial cells. Two cDNA libraries of up- and downregulated genes were generated, comprising 253 and 299 clones, respectively. The sequence analysis revealed 205 different known genes and 314 cDNA fragments of unknown functions. Northern-blot analysis of 167 clones confirmed differential expression in 58%, and indicated a similar expression pattern in additional lung-cancer cell lines for selected clones, strengthening the value of this model for the identification of new candidate genes in lung carcinogenesis.
authors.affiliation: Institute of Pathology, University Hospital Charité, Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Petersen
authors.forename: C
authors.initials: C
authors.lastname: Heckert
authors.forename: J
authors.initials: J
authors.lastname: Rudolf
authors.forename: K
authors.initials: K
authors.lastname: Schlüns
authors.forename: O I
authors.initials: OI
authors.lastname: Tchernitsa
authors.forename: R
authors.initials: R
authors.lastname: Schäfer
authors.forename: M
authors.initials: M
authors.lastname: Dietel
authors.forename: I
authors.initials: I
authors.lastname: Petersen
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured