title: Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization.
abstract: To investigate the population structure of the predominant phylogenetic groups within the human adult fecal microbiota, a new oligonucleotide probe designated S-G-Clept-1240-a-A-18 was designed, validated, and used with a set of five 16S rRNA-targeted oligonucleotide probes. Application of the six probes to fecal samples from 27 human adults showed additivity of 70% of the total 16S rRNA detected by the bacterial domain probe. The Bacteroides group-specific probe accounted for 37% +/- 16% of the total rRNA, while the enteric group probe accounted for less than 1%. Clostridium leptum subgroup and Clostridium coccoides group-specific probes accounted for 16% +/- 7% and 14% +/- 6%, respectively, while Bifidobacterium and Lactobacillus groups made up less than 2%.
authors.affiliation: Institut National de Recherche Agronomique, Unité d'Ecologie et Physiologie du Système Digestif, Domaine de Vilvert, 78352 Jouy-en-Josas, France. sghir@genoscope.cns.fr
authors.forename: A
authors.initials: A
authors.lastname: Sghir
authors.forename: G
authors.initials: G
authors.lastname: Gramet
authors.forename: A
authors.initials: A
authors.lastname: Suau
authors.forename: V
authors.initials: V
authors.lastname: Rochet
authors.forename: P
authors.initials: P
authors.lastname: Pochart
authors.forename: J
authors.initials: J
authors.lastname: Dore
mesh_terms: D000328:Adult; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001439:Bacteroides / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D001644:Bifidobacterium / Q000235:genetics / Q000302:isolation & purification; D003013:Clostridium / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization / Q000379:methods; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S / Q000032:analysis
chemical_list: D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S