Changes in urine 8-hydroxydeoxyguanosine levels of super-marathon runners during a four-day race period.
We have determined the urinary 8-hydroxydeoxyguanosine (8-OHdG) levels of five well trained supra-marathon runners during a four-day race. The daily running distances of the four-day race were the following; 93 km, 120 km, 56 km and 59 km, respectively. Pre-race and post-race urine samples were collected on each day and analyzed by a monoclonal antibody technique. The urinary 8-OHdG content increased significantly on the first day and tended to decrease from the third day. By the fourth day 8-OHdG content was significantly less than measured on the first three days. The serum creatine kinase activity changed in a similar fashion, showing a large increase (P<0.001) up to the third day when it decreased significantly from the peak value (P<0.05). We conclude that extreme physical exercise causes oxidative DNA damage to well trained athletes. However, repeated extreme exercise-induced oxidative stress does not propagate on increase of urinary 8-OHdG, but rather causes an adaptation leading to normalization of oxidative DNA damage.
Radák
Z
Z
Lab. Exercice Physiol., Hungarian University of Physical Education, Budapest. radak@mte.hupe.hu
Pucsuk
J
J
Boros
S
S
Josfai
L
L
Taylor
A W
AW
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D003402:Creatine Kinase / Q000097:blood; D004249:DNA Damage / Q000502:physiology; D003681:Dehydration / Q000652:urine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology*; D012420:Running / Q000502:physiology*; D013997:Time Factors
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003402:Creatine Kinase; D003849:Deoxyguanosine