Differential expression of ras in organs and embryos of shrimp Penaeus monodon (Crustacea: Decapoda).
Total RNA from shrimp hepatopancreas of Penaeus monodon showed three prominent bands that react with the shrimp ras probe, a 239-bp product, of approximately 4.8 kb (R1), 3.1 kb (R2) and 1.3 kb (R3) on the northern blot. The R1 is the least abundant. Analyses of total RNA from gill and heart were similar to each other. The highest expression of Ras was observed in the gill, while a negligible signal was detected with the Ras probe in muscle. Ras expression is developmentally regulated in embryonic stages of shrimp. Messenger RNA levels of ras were increased from a minimum in the nauplius stage to a maximum in the post-larvae stage for R1 and R2. R3 showed a maximum at the protozoea stage. On the other hand, the activity of protein geranylgeranyltransferase I was increased significantly in the early nauplius stage. No correlative increase of prenylation activity by protein geranylgeranyltransferase I was observed with the transcription activity of ras.
Huang
C F
CF
Department of Zoology, National Taiwan University, Nankang, Taipei, Taiwan.
Chen
W Y
WY
Chuang
N N
NN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015342:DNA Probes; D018507:Gene Expression Regulation, Developmental; D011905:Genes, ras; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017368:Protein Prenylation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D018631:ras Proteins / Q000378:metabolism
D015342:DNA Probes; D012333:RNA, Messenger; D018631:ras Proteins