Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus.
A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, CbxR, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.
Honda
Y
Y
Wood Research Institute, Kyoto University, Gokasho Uji, Japan.
Matsuyama
T
T
Irie
T
T
Watanabe
T
T
Kuwahara
M
M
D019943:Amino Acid Substitution; D015139:Blotting, Southern; D002261:Carboxin / Q000494:pharmacology*; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005819:Genetic Markers; D007506:Iron-Sulfur Proteins / Q000235:genetics; D010957:Plasmids; D020076:Pleurotus / Q000187:drug effects / Q000235:genetics*; D017354:Point Mutation; D013385:Succinate Dehydrogenase / Q000235:genetics; D014170:Transformation, Genetic
D004271:DNA, Fungal; D004274:DNA, Recombinant; D005659:Fungicides, Industrial; D005819:Genetic Markers; D007506:Iron-Sulfur Proteins; D002261:Carboxin; D013385:Succinate Dehydrogenase