Molecular cloning, spatial and temporal characterization of spinach SOGA1 cDNA, encoding an alpha subunit of G protein.
Heterotrimeric G proteins are an important component of signal transduction pathway in animals. Although these proteins have been described in plants, their exact function and action mode are not clearly defined. In order to analyze the relationship between these proteins and the transduction of light signals in spinach, we have isolated by 5' and 3' RACE-PCR a 1660bp cDNA clone called SOGA1. This codes for a 383aa protein, which reveals a very strong homology with other plant Galpha subunit sequences. Genomic analysis suggested that SOGA1 belonged to a small multiple gene family. Northern blots and in-situ hybridization analyses showed that SOGA1 transcripts accumulate in all organs tested with a specific high level associated with the apex, roots and hypocotyls. Finally, a time-course analysis performed on the green tissues showed that accumulation of SOGA1 transcripts follows a circadian rhythm. However, in-situ hybridization analysis of the apex suggested the opposite behavior, while no variation was observed in the hypocotyl.
Perroud
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PF
Laboratoire de Biochimie et Physiologie Végétales, Université de Genève, place de l'Université 3, CH-1211, Geneva, Switzerland.
Diogon
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Crèvecoeur
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Greppin
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D018744:DNA, Plant / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008027:Light; D008969:Molecular Sequence Data; D017440:Photoperiod; D010802:Phylogeny; D010944:Plants / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D018724:Spinacia oleracea / Q000235:genetics* / Q000254:growth & development; D014018:Tissue Distribution; D014158:Transcription, Genetic / Q000528:radiation effects
D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant; D020962:Heterotrimeric GTP-Binding Proteins