The fractionation of t-RNA on N,N'-bis(3-aminopropyl)-piperazine substituted-Sepharose.
An anion exchange agarose has been prepared by modifying sepharose 6B with N,N'-bis (-3-aminopropyl) piperazine. This material (BAPP-Sepharose) has been used for the fractionation of t-RNA from E.coli by column chromatography. The results obtained with gram quantities of crude t-RNA at pH 4.6 and pH 8.0 as measured by the elution patterns of alanyl, arginyl, aspartyl, leucyl, lysyl, methionyl, phenylalanyl, prolyl, seryl, tyrosyl, and valyl t-RNA are described.
Leberman
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R
Giovanelli
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Acosta
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D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D010879:Piperazines; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
C402767:4-bis(3-aminopropyl)piperazine; D010879:Piperazines; D012329:RNA, Bacterial; D012685:Sepharose; D012343:RNA, Transfer