Generation of MHC-peptide tetramers: a new opportunity for dissecting T-cell immune responses.
In the past few years, the technical breakthrough in generating MHC-peptide tetramers has revolutionized the analysis of T-cell responses. The major advantage of this technique over currently available methods is the ability of these tetramers to label T lymphocytes according to their antigenic specificity. The present review describes some technical aspects of tetramers generation and discusses some of the numerous possibilities opened up by this new technology.
Bousso
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Unité de biologie moléculaire du gène, Inserm U277, Institut Pasteur, Paris, France.
D000818:Animals; D001419:Bacteria / Q000276:immunology; D001424:Bacterial Infections / Q000276:immunology / Q000382:microbiology; D006649:Histocompatibility Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000276:immunology; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014777:Virus Diseases / Q000276:immunology / Q000821:virology; D014780:Viruses / Q000276:immunology
D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D011948:Receptors, Antigen, T-Cell