Conformationally restricted carbohydrate-modified nucleic acids and antisense technology.
The study of conformationally restricted carbohydrate modified nucleic acids has given new insights into the concept of the antisense technology. We learned to understand the structural requirements of a modified nucleic acid to function as steric blocker for RNA. Several of the physicochemical and conformational factors influencing duplex stabilization are analyzed with respect to their relative importance for the antisense field.
Herdewijn
P
P
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium. piet.herdewijn@rega.kuleuven.ac.be
D020029:Base Pairing / Q000187:drug effects; D002241:Carbohydrates / Q000737:chemistry*; D002474:Cell-Free System / Q000378:metabolism; D002478:Cells, Cultured; D015195:Drug Design; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000187:drug effects; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D013329:Structure-Activity Relationship; D013402:Sugar Alcohols / Q000737:chemistry; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D002241:Carbohydrates; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D013402:Sugar Alcohols; D018799:Intercellular Adhesion Molecule-1