Molecular characterization of a calmodulin-like dictyostelium protein CalB.
A gene named calB was cloned and characterized in Dictyostelium. A relationship to calmodulin (CaM) is suggested by sequence identity (50%), similar exon-intron structure and cross-reactivity with anti-CaM sera. The level of calB mRNA is developmentally regulated with maxima during aggregation and in spores. CalB null cells grow normally, develop and produce viable spores. We demonstrated the capacity of tagged CalB to bind Ca(2+) using the (45)Ca(2+) overlay assay and showed that its mobility on SDS-PAGE is dependent on Ca(2+)/EGTA pretreatment.
Rösel
D
D
Department of Physiology and Developmental Biology, Charles University, Vinicná 7, Praha, Czech Republic.
Pûta
F
F
Blahůsková
A
A
Smýkal
P
P
Folk
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002147:Calmodulin / Q000235:genetics*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004023:Dictyostelium; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental; D015151:Immunoblotting; D008969:Molecular Sequence Data; D011485:Protein Binding; D015800:Protozoan Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
C410243:CalB protein, Dictyostelium; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D015800:Protozoan Proteins; D002118:Calcium