title: The solubility of the ribotoxin alpha-sarcin, produced as a recombinant protein in Escherichia coli, is increased in the presence of thioredoxin.
abstract: The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect disulphide bonds to produce the soluble native conformation. The protein thus produced retains the structural, spectroscopic and enzymatic features of the natural fungal alpha-sarcin.
authors.affiliation: Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: García-Ortega
authors.forename: J
authors.initials: J
authors.lastname: Lacadena
authors.forename: V
authors.initials: V
authors.lastname: Lacadena
authors.forename: M
authors.initials: M
authors.lastname: Masip
authors.forename: C
authors.initials: C
authors.lastname: De Antonio
authors.forename: A
authors.initials: A
authors.lastname: Martínez-Ruiz
authors.forename: A
authors.initials: A
authors.lastname: Martínez Del Pozo
mesh_terms: D001230:Aspergillus / Q000235:genetics / Q000378:metabolism; D004722:Endoribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005656:Fungal Proteins; D010957:Plasmids / Q000235:genetics; D011487:Protein Conformation; D011500:Protein Synthesis Inhibitors / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012995:Solubility; D013879:Thioredoxins / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011500:Protein Synthesis Inhibitors; D011994:Recombinant Proteins; C014801:alpha-sarcin; D013879:Thioredoxins; D004722:Endoribonucleases