The inhibitory effects of IFN alpha-2b on EGFR expression and growth of cultured subconjunctival fibroblasts.
To investigate to what extent the growth of cultured subconjunctival fibroblasts was regulated by epidermal growth factor receptor (EGFR), we observed the expression of EGFR inhibited by interferon alpha-2b (IFN alpha-2b) and determined the growth of cultured subconjunctival fibroblasts by immunohistochemical and MTT methods. The linear relationships between the concentration of IFN alpha-2b and EGFR expression or growth were y = 1252.7-554.61 lgx (r = -0.98, P < 0.01), y = 0.523-0.0421 lgx (r = -0.93, P < 0.05) respectively. The concentration of IFN alpha-2b at doubled IC50 to EGFR (6.55 x 10(3) IU/ml) only inhibited the cell growth by 25.13%. The results suggested that the growth of subconjunctival fibroblasts may be controlled by many other growth factors because it was only partially inhibited by decreasing EGFR expression.
Xiong
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X
Department of Ophthalmology, Xiehe Hospital, Tongji Medical University, Wuhan.
Wei
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H
Du
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D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003228:Conjunctiva / Q000166:cytology*; D066246:ErbB Receptors / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D011994:Recombinant Proteins
D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D066246:ErbB Receptors