A new antimitotic substance, FR182877. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities.
Using the characteristic morphological changes of mammalian cells, we screened novel antimitotic substances and found that a strain of Streptomyces sp. No.9885 produced FR182877. This substance was isolated from the culture broth by ethyl acetate extraction, silica gel column chromatography and ODS column chromatography. Structural studies on FR182877 suggested that it had a unique hexacyclic structure encompassing its highly strained double bond. FR182877 exhibited potent antitumor activities against murine ascitic tumor and solid tumor in vivo.
Sato
B
B
Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Tsukuba, Ibaraki, Japan.
Muramatsu
H
H
Miyauchi
M
M
Hori
Y
Y
Takase
S
S
Hino
M
M
Hashimoto
S
S
Terano
H
H
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005285:Fermentation; D006801:Humans; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000166:cytology / Q000187:drug effects; D007928:Lethal Dose 50; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008815:Mice, Inbred Strains; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009374:Neoplasms, Experimental / Q000188:drug therapy; D011083:Polycyclic Compounds / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013302:Streptomyces / Q000737:chemistry / Q000145:classification* / Q000378:metabolism*; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; C408303:FR 182877; D011083:Polycyclic Compounds