Inhibitory activity of the human papillomavirus type 1 AU-rich element correlates inversely with the levels of the elav-like HuR protein in the cell cytoplasm.
We have analysed the activity of the negative element in the late 3' untranslated region of the cutaneous human papillomavirus type 1 (HPV-1) mRNAs in a panel of epithelial cells, of both mucosal and cutaneous origin. The inhibitory activity was cell line dependent. Determination of the levels of heterogeneous ribonucleoprotein C1/C2 and HuR that have been shown previously to interact specifically with the HPV-1 negative RNA element revealed that low levels of the HuR protein in the cytoplasmic fraction correlated with high inhibitory activity of the HPV-1 element, whereas cell lines displaying low inhibitory activity contained high levels of HuR in the cytoplasm. These results suggested that the HuR protein has a positive effect on HPV-1 late gene expression.
Carlsson
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Department of Medical Biochemistry and Microbiology, Biomedical Center, Uppsala University, Sweden.
Schwartz
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D020413:3' Untranslated Regions / Q000235:genetics*; D000954:Antigens, Surface; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; D004847:Epithelial Cells; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics* / Q000502:physiology*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
D020413:3' Untranslated Regions; D000954:Antigens, Surface; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins