Isopycnic centrifugation of sheared L-cell chromatin in metrizamide gradients.
Isopycnic gradient centrifugation of L-929 cell chromatin in a 38% (W/V) metrizamide solution yields two distinct fractions. The fraction banding at a density of 1.24 gm/cm(2) (H chromatin) contains about 10% of the DNA present in the fraction banding at a density of 1.18 gm/cm(2) (L chromatin). Both fractions contain the same proportions of satellite to main band DNA's. Some differences can be seen in the DNA: protein ratios and types of proteins present in the H and L chromatin fractions.
Monahan
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D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002843:Chromatin / Q000737:chemistry* / Q000145:classification; D004247:DNA / Q000302:isolation & purification*; D004276:DNA, Satellite / Q000302:isolation & purification; D007739:L Cells / Q000737:chemistry*; D008793:Metrizamide / Q000737:chemistry*; D051379:Mice; D012313:RNA / Q000302:isolation & purification
D002843:Chromatin; D004276:DNA, Satellite; D012313:RNA; D004247:DNA; D008793:Metrizamide