Identification of variants and dual promoters of murine serine/threonine kinase KKIAMRE.
KKIAMRE is a serine/threonine protein kinase whose transcripts increase in the deep cerebellar nuclei of the rabbit after eyeblink conditioning, a model of associative learning and memory. We here characterized the expression, isoforms, and promoters of murine KKIAMRE gene. The expression of KKIAMRE was detected, by in situ hybridization and immunohistochemistry, in neurons in various brain regions including deep cerebellar nuclei. The gene spans approximately 40 kb and consists of 15 exons. Analysis of cDNA clones revealed multiple variants, having diversity in the putative carboxy-terminal regulatory domain, generated by alternative splicing and intraexonal termination. Furthermore, they had alternative 5' noncoding sequences. Primer extension, RNase protection, and transient expression assays revealed that two alternative promoters linked to distinct noncoding exons direct the expression of KKIAMRE. The gene was mapped on chromosomes 5 and 4 in mouse and human, respectively.
Sassa
T
T
Laboratory for Behavioral Genetics, Brain Science Institute, RIKEN, Saitama, Japan.
Gomi
H
H
Sun
W
W
Ikeda
T
T
Thompson
R F
RF
Itohara
S
S
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D018844:Cyclin-Dependent Kinases; D018076:DNA, Complementary / Q000235:genetics; D014644:Genetic Variation; D016678:Genome; D006801:Humans; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D011817:Rabbits; D014018:Tissue Distribution; D014162:Transfection
D018076:DNA, Complementary; D020033:Protein Isoforms; D008156:Luciferases; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C103570:CDKL2 protein, human; C521371:Cdkl2 protein, mouse; D018844:Cyclin-Dependent Kinases; D010751:Phosphopyruvate Hydratase