title: Murine colonic mucosa hyperproliferation. I. Elevated CFTR expression and enhanced cAMP-dependent Cl(-) secretion.
abstract: Fluid transport in the large intestine is mediated by the cystic fibrosis gene product and cAMP-dependent anion channel cystic fibrosis transmembrane conductance regulator (CFTR). cAMP-mediated Cl(-) secretion by gastrointestinal cell lines in vitro has been positively correlated with the insertion of CFTR into the apical membrane of differentiated senescent colonocytes and negatively correlated with the failure of CFTR to insert into the plasma membrane of their undifferentiated proliferating counterparts. In native tissues, this relationship remains unresolved. We demonstrate, in a transmissible murine colonic hyperplasia (TMCH) model, that (8-fold) colonocyte proliferation was accompanied by increased cellular CFTR mRNA and protein expression (8.3- and 2.4-fold, respectively) and enhanced mucosal cAMP-dependent Cl(-) secretion (2. 3-fold). By immunofluorescence microscopy, cellular CFTR expression was restricted to the apical pole of cells at the base of the epithelial crypt. In contrast, increased cellular proliferation in vivo led to increases in both the cellular level and the total number of cells expressing this anion channel, with cellular CFTR staining extending into the crypt neck region. Hyperproliferating colonocytes accumulated large amounts of CFTR in apically oriented subcellular perinuclear compartments. This novel mode of CFTR regulation may explain why high endogenous levels of cellular CFTR mRNA and protein within the TMCH epithelium were not matched with larger increases in transmucosal CFTR Cl(-) current.
authors.affiliation: Department of Integrative Biology, Pharmacology and Physiology, Division of Gastroenterology, Hepatology and Nutrition, The University of Texas Health Science Center at Houston, Medical School, Houston 77030, USA.
authors.forename: S
authors.initials: S
authors.lastname: Umar
authors.forename: J
authors.initials: J
authors.lastname: Scott
authors.forename: J H
authors.initials: JH
authors.lastname: Sellin
authors.forename: W P
authors.initials: WP
authors.lastname: Dubinsky
authors.forename: A P
authors.initials: AP
authors.lastname: Morris
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism*; D002954:Citrobacter; D016971:Citrobacter freundii; D005576:Colforsin / Q000494:pharmacology; D003106:Colon / Q000473:pathology / Q000503:physiopathology*; D000242:Cyclic AMP / Q000502:physiology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D005665:Furosemide / Q000494:pharmacology; D005786:Gene Expression Regulation; D006965:Hyperplasia; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D002712:Chlorides; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005576:Colforsin; D005665:Furosemide; D000242:Cyclic AMP