Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials.
Recently, resin-based dental restorative materials have been targeted as potential sources of xenoestrogens, specifically bisphenol A (BPA) and bisphenol A dimethacrylate (BAD), which could contribute to overall estrogen load and result in deleterious side effects. The present study used high-pressure liquid chromatography (HPLC) to analyze twenty-eight different commercially available dental resins for the presence of BPA and/or BAD. In addition, sublines of the MCF-7 human breast tumor cell line were cultured in the presence of eluates from eleven of the dental resins and assessed for proliferative responses using the sulforhodamine B assay. Only one resin, Delton II, had detectable levels of BPA or BAD that could be verified by Fourier transform infrared spectrometry. Likewise, eluates from Delton II were the only samples that elicited a significant proliferative response in two of the MCF-7 sublines tested. Therefore, we conclude that dental resins in general do not represent a significant source of BPA or BAD exposure.
Lewis
J B
JB
Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta 30912-1126, USA. jillewis@mail.mcg.edu
Rueggeberg
F A
FA
Lapp
C A
CA
Ergle
J W
JW
Schuster
G S
GS
D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate / Q000032:analysis / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003188:Composite Resins / Q000737:chemistry / Q000494:pharmacology; D003764:Dental Materials / Q000737:chemistry* / Q000494:pharmacology; D003793:Dental Restoration, Permanent; D004852:Epoxy Compounds / Q000032:analysis*; D004968:Estrogens, Non-Steroidal / Q000032:analysis*; D006801:Humans; D008689:Methacrylates / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D019279:Resin Cements / Q000737:chemistry / Q000494:pharmacology; D012117:Resins, Synthetic / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014407:Tumor Cells, Cultured; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D017438:Bisphenol A-Glycidyl Methacrylate; D003188:Composite Resins; D003764:Dental Materials; D004852:Epoxy Compounds; D004968:Estrogens, Non-Steroidal; D008689:Methacrylates; D010636:Phenols; D019279:Resin Cements; D012117:Resins, Synthetic; C002462:4,4'-isopropylidenediphenol dimethacrylate; D000098826:Bisphenol A Compounds; C006780:bisphenol A