Effect of plaunotol on bacterial translocation in the rat small intestine.
OBJECTIVE
Bacterial translocation is precipitated by an increase in bacteria or endotoxin, depression of the membrane barrier, and an increase in mucosal permeability. Plaunotol is a mucosal protective agent, and observed to have a strong suppressive effect on superoxide production. In this study, the effect of plaunotol on bacterial translocation was examined using the model of ischemia and reperfusion.


METHODS
Male Sprague Dawley rats were used to create the following model for evaluation of bacterial translocation: (i) the control group; (ii) the preventive dose group (plaunotol 30 mg/kg/day one week before surgery); (iii) the therapeutic dose group (plaunotol 30 mg/kg/day one week after surgery); and (iv) the full dose group (plaunotol 30 mg/kg/day one week before surgery and one week after surgery). Bacterial translocation was assessed as the blood concentration of the endotoxin.


RESULTS
In the control group, the endotoxin increased significantly 3 days postsurgery (13.7+/-5.6 pg/ml) compared with before surgery (1.1+/-0.1 pg/ml). In the preventive and full-dose groups, the erndotoxin decreased significantly 3 days postsurgery (4.4+/-2.8 pg/ml, 5.7+/-2.7 pg/ml, respectively) compared with that of the control group.


CONCLUSIONS
Plaunotol in the preventive and full-dose groups decreased the endotoxin. This suggests that plaunotol is one of the protectors for bacterial translocation.
Sakai
T
T
Second Department of Surgery, School of Medicine, Fukuoka University, Japan. sakai-t@rb3.so-net.ne.jp
Yamashita
Y
Y
Maekawa
T
T
Shirakusa
T
T
Okabe
N
N
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D018988:Bacterial Translocation / Q000187:drug effects*; D004195:Disease Models, Animal; D004224:Diterpenes; D004731:Endotoxins / Q000097:blood; D005233:Fatty Alcohols / Q000008:administration & dosage / Q000494:pharmacology*; D007421:Intestine, Small / Q000382:microbiology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000382:microbiology
D000900:Anti-Bacterial Agents; D004224:Diterpenes; D004731:Endotoxins; D005233:Fatty Alcohols; C047686:plaunotol