Bioactive amide of prostaglandin E1 and ethanolamine plasmalogen analog of platelet-activating factor inhibits several pathways of human platelet aggregation.
The influence of an amide of prostaglandin E1 and ethanolamine plasmalogen platelet-activating factor analog 1-O-alk-1;-enyl-2-acetyl-sn-glycero-3-phospho-(N-11alpha, 15alpha-dioxy-9-keto-13-prostenoyl)ethanolamine (PGE1-PPAF) on platelet-activating factor (PAF)-, ADP-, and thrombin-induced human platelet aggregation has been studied. It was found that PGE1-PPAF inhibits the PAF-, ADP-, and thrombin-induced platelet aggregation in platelet-rich plasma. 1-O-alk-1;-enyl-2-acetyl-sn-glycero-3-phosphoethanolamine inhibited PAF-induced aggregation up to 50% but had no influence on platelet aggregation induced by ADP or thrombin. The ethanolamine plasmalogen analog of PAF 1-O-alk-1;-enyl-2-acetyl-sn-glycero-3-phospho-(N-palmitoyl)ethanolami ne, having a palmitoyl residue instead of PGE1, did not inhibit platelet aggregation induced by PAF, ADP, or thrombin. We propose that inhibition of human platelet aggregation by PGE1-PPAF is mediated by its action on platelet PAF-receptors and the adenylate cyclase system.
Kulikov
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Research and Development Center for Medical Biotechnology, Ministry of Public Health of the Russian Federation, Moscow, 123182, Russia.
Muzya
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GI
D000527:Alprostadil / Q000737:chemistry / Q000378:metabolism; D000577:Amides / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000494:pharmacology*; D010955:Plasmalogens / Q000737:chemistry / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D013917:Thrombin / Q000494:pharmacology
D000577:Amides; D010714:Phosphatidylethanolamines; D010955:Plasmalogens; D010972:Platelet Activating Factor; C020791:phosphatidal ethanolamines; D013917:Thrombin; D000527:Alprostadil