title: Distribution of fibronectins and their integrin receptors in interface tissue from aseptic loosening of hip prostheses.
abstract: OBJECTIVE
  To assess the distribution of fibronectins (FNs) and their integrin (Int) receptors in synovial membrane-like interface tissue (SMLIT) from aseptic loosening of total hip replacement (THR), and potential role of FN-Int interaction in the loosening process.
  
  
  METHODS
  The alkaline phosphatase anti-alkaline phosphatase (APAAP) method was used to detect the distribution of FNs and their Int receptors in SMLIT and control samples. Double immunofluorescence labeling was used to reveal the different co-localizations.
  
  
  RESULTS
  Intensive FN staining appeared in the lining layers, sublining area, and vascular endothelium, while immunoreactivities for Int alpha 4, alpha 5, beta 1 subunits were detected in the lining and endothelial cells of SMLIT. Immunofluorescence labeling revealed Int alpha 5 and collagenase-1/collagenase-3 double positive cells in lining layers and sublining area of SMLIT.
  
  
  CONCLUSIONS
  Increased expression of FNs, Int alpha 4 beta 1 and alpha 5 beta 1 appeared in SMLIT compared with that in OA synovial membrane. FN-Int interactions may play a role in local collagenase production.
authors.affiliation: Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Finland.
authors.forename: T F
authors.initials: TF
authors.lastname: Li
authors.forename: J W
authors.initials: JW
authors.lastname: Xu
authors.forename: S
authors.initials: S
authors.lastname: Santavirta
authors.forename: L
authors.initials: L
authors.lastname: Nordsletten
authors.forename: O
authors.initials: O
authors.lastname: Michelsson
authors.forename: M
authors.initials: M
authors.lastname: Takagi
authors.forename: I
authors.initials: I
authors.lastname: Virtanen
authors.forename: Y T
authors.initials: YT
authors.lastname: Konttinen
mesh_terms: D019644:Arthroplasty, Replacement, Hip; D017364:Collagenases / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005353:Fibronectins / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006622:Hip Prosthesis; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13; D010003:Osteoarthritis / Q000601:surgery; D011475:Prosthesis Failure; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin / Q000378:metabolism*; D012086:Reoperation; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology
chemical_list: D005353:Fibronectins; D016023:Integrins; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D017364:Collagenases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13