Automated determination of reboxetine by high-performance liquid chromatography with column-switching and ultraviolet detection.
A fully automated method including column-switching and isocratic high-performance liquid chromatography (HPLC) was developed for quantitative analysis of the new antidepressant reboxetine, a noradrenaline reuptake inhibitor. After serum injection into the HPLC system and on-line sample clean-up on a silica C8 (10x4.0 mm I.D.) clean-up column with an eluent consisting of 2.5% acetonitrile in deionized water, the chromatographic separation was performed on an analytical column (Lichrospher CN; 250x4.6 mm I.D.) with an eluent of acetonitrile-aqueous potassium phosphate buffer (0.008 M, pH 6.4) (50:50). The UV detector was set at 273 or 226 nm. The limit of quantification was about 15 ng/ml at 273 nm and about 4 ng/ml at 226 nm. The day-to-day relative standard deviation ranged between 2.7 and 6.7% with recovery rates > or = 90%. Linear regression analyses revealed correlation coefficients > 0.998. The method can be applied to therapeutic drug monitoring of reboxetine as well as pharmacokinetic studies.
Härtter
S
S
Department of Psychiatry, University of Mainz, Germany.
Weigmann
H
H
Hiemke
C
C
D018759:Adrenergic Uptake Inhibitors / Q000032:analysis / Q000097:blood; D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009025:Morpholines / Q000032:analysis* / Q000097:blood; D011786:Quality Control; D000077593:Reboxetine; D013056:Spectrophotometry, Ultraviolet
D018759:Adrenergic Uptake Inhibitors; D009025:Morpholines; D000077593:Reboxetine