[Conformational changes in C3 levels of complement during storage of blood from Hodgkin's disease patients and from healthy people].
Storage-related differences in C3 level of blood complement and its hydrolized form--C3(H2O)--were identified in Hodgkin's disease patients and healthy donors, by immunoenzymatic analysis using murine monoclonal antibodies. Both C3 and C3(H2O) levels in blood serum of patients varied with time and were significantly different from those in health subjects; they correlated with EDTA concentration. After a second thawing of plasma in patients, there were no traces left of C3(H2O).
Kniazeva
O A
OA
Research Center, Russian Academy of Sciences.
Sakaeva
D D
DD
Tiagotin
Iu V
IuV
D000911:Antibodies, Monoclonal; D000925:Anticoagulants / Q000097:blood; D001793:Blood Preservation; D016022:Case-Control Studies; D003176:Complement C3 / Q000378:metabolism*; D015925:Cryopreservation; D004492:Edetic Acid / Q000097:blood; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D006868:Hydrolysis; D007124:Immunoenzyme Techniques; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000925:Anticoagulants; D003176:Complement C3; D004492:Edetic Acid