The metabolism of isocytidine in Escherichia coli.
Intact cells and cell-free extracts of E. coli convert isocytidine to isocytosine and uracil. The radioactive label of 5-[(3)H]isocytidine is incorporated into RNA and, DNA of growing bacteria at a rate equal to about 1.4% of that of cytidine under similar conditions; the radioactivity is found in uridylic, cytidylic and 2'-deoxythymidylic acids, while less than 0.4% of incorporated radioactive material might be due to possible incorporation of intact isocytidine. Uridine phosphorylase and cytidine deaminase apparently do not participate in the metabolic conversion of isocytidine.5-[(3)H]isocytidine was prepared by platinum-catalyzed dehalogenation of 5-bromoisocytidine in the presence of tritium. The 5-bromo derivative was obtained from 2',3'-0- -isopropylideneisocytidine by N-bromsuccinimide bromination followed by acidic hydrolysis.
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D001426:Bacterial Proteins / Q000378:metabolism; D002474:Cell-Free System; D003562:Cytidine / Q000378:metabolism*; D043525:Cytosine Deaminase; D004269:DNA, Bacterial / Q000378:metabolism; D003641:Deamination; D004926:Escherichia coli / Q000378:metabolism*; D009700:Nucleoside Deaminases / Q000378:metabolism; D010766:Phosphorylation; D012329:RNA, Bacterial / Q000378:metabolism; D014543:Uridine Phosphorylase / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; C083576:isocytidine; D003562:Cytidine; D014543:Uridine Phosphorylase; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase