Role of melanotransferrin (p97) in non-transferrin iron uptake by HeLa and K562 cells.
We tested whether melanotransferrin (p97), an iron-binding protein of the plasma membrane, is involved in the transport of non-transferrin iron into human HeLa and K562 cells. The expression of melanotransferrin was detected in HeLa but not in K562 cells. PI-PLC treatment dramatically decreased (to 20% of the original value) p97 expression by HeLa cells. However, the rate of iron uptake from 55Fe-ferric citrate by PI-PLC-treated HeLa cells was comparable or only slightly lower (80-100%) than the rate of iron uptake by untreated cells. PI-PLC treatment had no effect on iron uptake by K562 cells. These findings strongly support the suggestion that melanotransferrin does not play a substantial role in non-transferrin iron uptake by either HeLa or K562 cells.
Kriegerbecková
K
K
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Kovár
J
J
D000951:Antigens, Neoplasm; D001692:Biological Transport; D005290:Ferric Compounds / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000378:metabolism*; D020014:K562 Cells; D058950:Melanoma-Specific Antigens; D008565:Membrane Proteins / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010738:Type C Phospholipases / Q000494:pharmacology
D000951:Antigens, Neoplasm; D005290:Ferric Compounds; D058950:Melanoma-Specific Antigens; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C025314:ferric citrate; D007501:Iron; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase