Characterization of a Mycobacterium smegmatis mutant that is simultaneously resistant to D-cycloserine and vancomycin.
A mutant of Mycobacterium smegmatis has been isolated that is simultaneously resistant to both D-cycloserine (D-CS) and vancomycin. Genetic complementation with a PBP4 homolog restores sensitivity to both drugs. Resistance to D-CS and vancomycin in this mutant is most likely due to a novel mechanism involving peptidoglycan assembly at the cell surface.
Peteroy
M
M
Department of Microbiology and Molecular Genetics, UMDNJ/New Jersey Medical School, Newark, New Jersey 07103-2714, USA.
Severin
A
A
Zhao
F
F
Rosner
D
D
Lopatin
U
U
Scherman
H
H
Belanger
A
A
Harvey
B
B
Hatfull
G F
GF
Brennan
P J
PJ
Connell
N D
ND
D000595:Amino Acid Sequence; D003523:Cycloserine / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D008969:Molecular Sequence Data; D009154:Mutation; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000235:genetics*; D016415:Sequence Alignment; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance
D014640:Vancomycin; D003523:Cycloserine