title: Flow cytometric analysis of androgen receptor expression in human prostate tumors and benign tissues.
abstract: Androgen receptor (AR) plays an important role in growth and hormonal therapy of human prostate tumors. Immunohistochemical analysis of AR expression, a nonquantitative technique, is currently used for screening of receptor expression in prostate tissues. The present report describes a laser flow cytometric method for monitoring AR expression in human cell lines and in archival formalin-fixed paraffin-embedded prostate tissues and tumors. Multiparametric flow analysis can be used for simultaneous detection of other cellular markers (e.g., DNA aneuploidy), and by gated analysis, AR expression in subpopulations of a tumor can be quantitatively determined.
authors.affiliation: Division of Experimental Therapeutics, Sylvester Cancer Center, University of Miami School of Medicine, Florida 33136, USA.
authors.forename: A
authors.initials: A
authors.lastname: Krishan
authors.forename: A
authors.initials: A
authors.lastname: Oppenheimer
authors.forename: W
authors.initials: W
authors.lastname: You
authors.forename: R
authors.initials: R
authors.lastname: Dubbin
authors.forename: D
authors.initials: D
authors.lastname: Sharma
authors.forename: B L
authors.initials: BL
authors.lastname: Lokeshwar
mesh_terms: D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011467:Prostate / Q000737:chemistry* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011944:Receptors, Androgen / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D011944:Receptors, Androgen