Removal of xenoreactive antibodies by protein-A immunoadsorption: experience in 22 patients.
The presence of naturally occurring anti-Galalpha1-3Gal (antialphaGal) Ab in human serum is believed to be a major factor in the pathogenesis of hyperacute rejection of discordant organ xenografts such as the pig-to-human combination. Galalpha1-3Gal epitopes are expressed on pig tissues and the binding of anti-Galalpha1-3Gal leads to endothelial cell activation and complement-mediated hyperacute graft rejection. Several strategies have been suggested in donor animals or in the xenograft recipient to overcome the anti-alphaGal barrier. Protein-A immunoadsorption (PAIA) was developed for the in vivo removal of circulating Ab and it has been shown to be effective in cases where pathogenic auto or alloAb are present. The aim of our study was to analyze the effect of PAIA on total and xenoreactive serum anti-alphaGal immunoglobulin levels in a group of patients treated with this technique for different diseases. After three consecutive sessions of PAIA, total and xenoreactive IgG and IgM immunoglobulin levels were decreased by more than 50% of pre-treatment levels. So we conclude that PAIA is an effective method to significantly reduce circulating Ab, including xenogeneic IgM and IgG Ab. This mode of therapy might be considered as a tool to overcome hyperacute xenograft rejection. PAIA combined with other therapeutic approaches may well protect the xenograft.
Ramos
A
A
Nephrology Department, Marqués de Valdecilla Hospital, University of Cantabria, Santander, Spain.
Ruiz
J C
JC
de Francisco
A L
AL
Gómez-Fleitas
M
M
Arias
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000910:Antibodies, Heterophile / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D000920:Antibody-Dependent Cell Cytotoxicity; D002460:Cell Line; D003165:Complement System Proteins / Q000378:metabolism; D004187:Disaccharides / Q000097:blood / Q000276:immunology; D000939:Epitopes / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D013205:Staphylococcal Protein A
D000910:Antibodies, Heterophile; D004187:Disaccharides; D000939:Epitopes; D007136:Immunoglobulins; D007164:Immunosorbents; D013205:Staphylococcal Protein A; C055075:galactosyl-(1-3)galactose; D003165:Complement System Proteins