[Development of human preantral follicles and oocytes maturation in vitro].
OBJECTIVE
To study the development of human preantral follicles and oocytes maturation by in vitro culture.


METHODS
Preantral follicles obtained from follicular fluid during egg retrieval in an in vitro fertilization (IVF) program was first incubated in Ham's F10 with 15% fetal cord serum (FCS). After 28 days, the medium was supplemented with different doses of hMG, mature follicular fluid (mFF) and epidermal growth factor (EGF) by orthogonal design. Induction of final maturation was completed in the presence of human menopasual gonadotropin hMG and mFF.


RESULTS
Development from preantral to antral follicles was found within 6-12 days of culture. With increasing time, the proportion of follicles with diameters of more than 300 micrograms increased at 21-28 days of culture (P < 0.005). The maximal number of oocytes extrusion and first polar body formation occurred in the presence of 1501U/L hMG, 40% (v/v) mFF and 6 micrograms/L EGF.


CONCLUSIONS
Follicular fluid obtained during egg retrieval in an IVF program was found to contain many preantral follicles which could develop into antral follicles with extrusion of oocytes in culture, and the oocytes matured in vitro. However, the course of development of follicles and maturation of oocytes in vitro is quite different from that in vivo.
Wu
J
J
Third Clinical Medical College, Beijing Medical University.
Zhang
L
L
Liu
P
P
D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000502:physiology; D006801:Humans; D008596:Menotropins / Q000494:pharmacology; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*
D008596:Menotropins; D004815:Epidermal Growth Factor