title: Epidemiological typing of Bordetella pertussis isolates: recommendations for a standard methodology.
abstract: Pertussis is re-emerging in vaccinated populations, and to gain insight into the reasons for this development population-based studies are necessary. Unfortunately, various techniques are used to study Bordetella pertussis populations, hampering comparison between studies. A standard methodology for epidemiological typing of Bordetella pertussis isolates is proposed which is based on serotyping, pulsed-field gel electrophoresis and gene typing. Such a standard approach will allow comparisons between studies performed in different laboratories. Comparisons may reveal whether the epidemiological differences observed between countries are due for instance to different Bordetella pertussis populations or different vaccines used.
authors.affiliation: Research Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. fr.mooi@rivm.nl
authors.forename: F R
authors.initials: FR
authors.lastname: Mooi
authors.forename: H
authors.initials: H
authors.lastname: Hallander
authors.forename: C H
authors.initials: CH
authors.lastname: Wirsing von König
authors.forename: B
authors.initials: B
authors.lastname: Hoet
authors.forename: N
authors.initials: N
authors.lastname: Guiso
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000592:standards*; D001483:Base Sequence; D001886:Bordetella pertussis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000592:standards; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008969:Molecular Sequence Data; D037342:Pertussis Toxin; D011110:Polymorphism, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D012703:Serotyping / Q000592:standards; D013048:Specimen Handling; D010566:Virulence Factors, Bordetella / Q000235:genetics; D014917:Whooping Cough / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins; D010566:Virulence Factors, Bordetella; C083388:pertussis toxin, S1 subunit; C068929:pertactin; D037342:Pertussis Toxin