Murine model study of the practical implication of trypanosome-induced immunosuppression in vaccine-based disease control programmes.
The relevance of trypanosome-induced immunosuppression in relation to the efficacy of vaccine-induced immunity was studied in mice. Mice were immunised with crude Trichinella spiralis muscle larvae homogenate vaccine and infected with T. spiralis and/or Trypanosoma brucei. Vaccination significantly decreased adult worm burden (p<0. 05) and accelerated worm expulsion in mice infected with T. spiralis only. T. brucei superinfection resulted in monocytosis, suppressed eosinophilia, significant decrease in PCV (p<0.001), higher numbers of adult worms (p<0.001) and failure to expel all adult worms by Day 12 post infection (p.i.). Regardless, they produced anti-Trichinella IgG(1) responses similar to those of the vaccinated non-T. brucei-infected group. T. brucei also suppressed the proliferative responses of spleen cells to stimulation with Con A and T. spiralis antigen, and induced strong production of interferon-gamma (IFN-gamma) in culture supernatants of antigen stimulated spleen and mesenteric lymph node cells. Interleukin-5 (IL-5) production was suppressed by T. brucei in supernatants of Con A- and antigen-stimulated spleen cells. It was concluded that trypanosome infections and the associated immunosuppression are of great practical significance in trypanosome endemic areas, especially with regards to disease control programmes involving vaccine-induced herd immunity.
Onah
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DN
School of Biological Sciences, University of Nottingham, UK. denis@fc.miyazaki-med.ac.jp
Wakelin
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D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D016207:Cytokines / Q000032:analysis; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006400:Hematocrit; D007108:Immune Tolerance / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D018448:Models, Immunological; D009000:Monocytes / Q000276:immunology; D018512:Parasitemia / Q000276:immunology / Q000662:veterinary; D016052:Protozoan Vaccines / Q000276:immunology*; D014234:Trichinella / Q000276:immunology; D014346:Trypanosoma brucei brucei / Q000254:growth & development / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control* / Q000662:veterinary
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D016052:Protozoan Vaccines; D003208:Concanavalin A