title: Production of very-high-amylose potato starch by inhibition of SBE A and B.
abstract: High-amylose starch is in great demand by the starch industry for its unique functional properties. However, very few high-amylose crop varieties are commercially available. In this paper we describe the generation of very-high-amylose potato starch by genetic modification. We achieved this by simultaneously inhibiting two isoforms of starch branching enzyme to below 1% of the wild-type activities. Starch granule morphology and composition were noticeably altered. Normal, high-molecular-weight amylopectin was absent, whereas the amylose content was increased to levels comparable to the highest commercially available maize starches. In addition, the phosphorus content of the starch was increased more than fivefold. This unique starch, with its high amylose, low amylopectin, and high phosphorus levels, offers novel properties for food and industrial applications.
authors.affiliation: Unilever Research Colworth, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK.
authors.forename: G P
authors.initials: GP
authors.lastname: Schwall
authors.forename: R
authors.initials: R
authors.lastname: Safford
authors.forename: R J
authors.initials: RJ
authors.lastname: Westcott
authors.forename: R
authors.initials: R
authors.lastname: Jeffcoat
authors.forename: A
authors.initials: A
authors.lastname: Tayal
authors.forename: Y C
authors.initials: YC
authors.lastname: Shi
authors.forename: M J
authors.initials: MJ
authors.lastname: Gidley
authors.forename: S A
authors.initials: SA
authors.lastname: Jobling
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000037:antagonists & inhibitors / Q000235:genetics*; D000687:Amylopectin / Q000032:analysis; D000688:Amylose / Q000032:analysis / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods; D016373:DNA, Antisense; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics; D030821:Plants, Genetically Modified; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D013213:Starch / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D016373:DNA, Antisense; D007527:Isoenzymes; D013213:Starch; D000688:Amylose; D000687:Amylopectin; D015061:1,4-alpha-Glucan Branching Enzyme