Purification and characterization of biosurfactants from Nocardia sp. L-417.
The Nocardia sp. L-417 strain grown with n-hexadecane as a carbon source produced two types of biosurfactant that have different characteristics. These biosurfactants were purified by procedures that included ammonium sulphate fractionation, chilled acetone and hexane treatments, silica-gel column chromatography and Sephadex LH-20 gel filtration. The purified biosurfactants were very stable over a broad range of pHs (2-12) and temperatures (100 degrees C, 3 h). The biosurfactant type I had strong properties as an emulsifying agent and as an emulsion-stabilizing agent, whereas type II had a strong ability to reduce surface tension.
Kim
S H
SH
Department of Microbiology, College of Natural Sciences, Pusan National University, Pusan 609-735, Korea.
Lim
E J
EJ
Lee
S O
SO
Lee
J D
JD
Lee
T H
TH
D002853:Chromatography, Liquid / Q000379:methods; D004655:Emulsions; D005079:Excipients / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009615:Nocardia / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
D004655:Emulsions; D005079:Excipients; D013501:Surface-Active Agents