Characterization of a sapphire-epoxy coating for capillary electrophoresis.
A new procedure for coating capillaries for capillary electrophoresis applying a sapphire (alumina) containing epoxy resin was developed. Coated capillaries showed considerably reduced electroosmotic flow, and decreased the adsorption of proteins to the internal wall of the capillary. Coating is transparent down to 195 nm and can be used with advantage to analyze different kinds of substances, such as small cations and/or anions, and proteins.
Bocaz
G
G
Masaryk University, Faculty of Science, Department of Analytical Chemistry, Brno, Czech Republic.
Revilla
A L
AL
Krejcí
J
J
Havel
J
J
D000327:Adsorption; D000537:Aluminum Oxide; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D004852:Epoxy Compounds; D019793:Fluorescein; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012116:Resins, Plant
D004852:Epoxy Compounds; D011506:Proteins; D012116:Resins, Plant; D009113:Muramidase; D000537:Aluminum Oxide; D019793:Fluorescein