A functional cra gene is required for Salmonella enterica serovar typhimurium virulence in BALB/c mice.
A minitransposon mutant of Salmonella enterica serovar Typhimurium SR-11, SR-11 Fad(-), is unable to utilize gluconeogenic substrates as carbon sources and is avirulent and immunogenic when administered perorally to BALB/c mice (M. J. Utley et al., FEMS Microbiol. Lett., 163:129-134, 1998). Here, evidence is presented that the mutation in SR-11 Fad(-) that renders the strain avirulent is in the cra gene, which encodes the Cra protein, a regulator of central carbon metabolism.
Allen
J H
JH
Department of Biochemistry, Microbiology, and Molecular Genetics, University of Rhode Island, Kingston, Rhode Island 02881, USA.
Utley
M
M
van Den Bosch
H
H
Nuijten
P
P
Witvliet
M
M
McCormick
B A
BA
Krogfelt
K A
KA
Licht
T R
TR
Brown
D
D
Mauel
M
M
Leatham
M P
MP
Laux
D C
DC
Cohen
P S
PS
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D050260:Carbohydrate Metabolism; D002952:Citric Acid Cycle; D005798:Genes, Bacterial; D007422:Intestines / Q000382:microbiology; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009093:Mucus / Q000382:microbiology; D016254:Mutagenesis, Insertional; D012097:Repressor Proteins / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D012703:Serotyping; D056968:Lac Repressors
D001426:Bacterial Proteins; D012097:Repressor Proteins; C068837:FruR protein, Bacteria; D056968:Lac Repressors