title: Artificial tear adsorption on soft contact lenses: methods to test surfactant efficacy.
abstract: Spoilage is a primary factor in the biocompatibility of soft contact lenses (SCL) within the lacrimal fluid. Tears are a complex mixture of proteins, lipids, natural surfactants and salts. The spoilation process is due to a contribution of all these components and of the nature of SCL materials themselves. The aim of this study was to set up methods to observe and quantify lacrimal deposits and to select efficient surfactants for preventing protein deposits. The present study was performed on PMMA-NVP SCL. The behaviour of SCL in presence of tears was studied by means of an in vitro artificial tear model consisting of the main tears components and quantified by a colorimetric technique (BCA) performed directly on the lenses. The nature of the deposit was observed directly by atomic force microscopy (AFM) in a liquid medium showing the same adsorption trend noticed in the quantitative results and identifying specific adsorption sites. The assessment of surfactant adsorption was performed using Maron's method, as a mean to evaluate the affinity of surfactant to the surface, while the action of selected surfactants on pre-treated SCL was assessed using the BCA method. Promising results were obtained with these two different methods which can be used easily for the pre-selection of surfactants for further cleaning solution formulation studies.
authors.affiliation: Université Claude Bernard, Villeurbanne, France.
authors.forename: V
authors.initials: V
authors.lastname: Rebeix
authors.forename: F
authors.initials: F
authors.lastname: Sommer
authors.forename: B
authors.initials: B
authors.lastname: Marchin
authors.forename: D
authors.initials: D
authors.lastname: Baude
authors.forename: M D
authors.initials: MD
authors.lastname: Tran
mesh_terms: D000327:Adsorption; D018441:Biofilms; D002021:Buffers; D003124:Colorimetry; D003263:Contact Lenses, Hydrophilic; D004341:Drug Evaluation; D004355:Drug Stability; D005960:Glucosides / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008768:Methylmethacrylates / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009883:Ophthalmic Solutions / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D011205:Povidone / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D015203:Reproducibility of Results; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D013863:Thioglucosides / Q000737:chemistry; D017155:Wettability
chemical_list: D002021:Buffers; D005960:Glucosides; D008055:Lipids; D008768:Methylmethacrylates; D009883:Ophthalmic Solutions; D011108:Polymers; D011136:Polysorbates; D011409:Propylene Glycols; D011506:Proteins; D013501:Surface-Active Agents; D013863:Thioglucosides; C012504:polypropylene glycol; C037334:poly(methylmethacrylate-N-vinylpyrrolidone); C018619:octyl-beta-D-glucoside; D011092:Polyethylene Glycols; C042989:n-octyl-beta-D-thioglucopyranoside; D011205:Povidone; C068430:polysorbate 40