Noninvasive study of radiation-induced oxidative damage using in vivo electron spin resonance.
Nitroxyl radicals injected into a whole body indicate the disappearance of signal intensity of in vivo electron spin resonance (ESR). The signal decay rates of nitroxyl have reported to be influenced by various types of oxidative stress. We examined the effect of X-irradiation on the signal decay rate of nitroxyl in the upper abdomen of mice using in vivo ESR. The signal decay rates increased 1 h after 15 Gy irradiation, and the enhancement was suppressed by preadministration of cysteamine, a radioprotector. These results suggest that the signal decay of nitroxyl in whole mice is enhanced by radiation-induced oxidative damage. The in vivo ESR system probing the signal decay of nitroxyl could provide a noninvasive technique for the study of oxidative stress caused by radiation in a living body.
Miura
Y
Y
Department of Biochemistry and Isotopes, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.
Ozawa
T
T
D000818:Animals; D003497:Cyclic N-Oxides / Q000493:pharmacokinetics; D003543:Cysteamine / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D051379:Mice; D009589:Nitrogen Oxides / Q000493:pharmacokinetics* / Q000528:radiation effects; D018384:Oxidative Stress / Q000528:radiation effects; D011759:Pyrrolidines / Q000493:pharmacokinetics; D011837:Radiation-Protective Agents / Q000494:pharmacology; D013113:Spin Labels
D003497:Cyclic N-Oxides; D009589:Nitrogen Oxides; D011759:Pyrrolidines; D011837:Radiation-Protective Agents; D013113:Spin Labels; C077426:3-carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl; D003543:Cysteamine; C039900:nitroxyl