Organization, nucleotide sequence, and chromosomal mapping of a tandemly repeated unit containing the four core histone genes and a 5S rRNA gene in an isopod crustacean species.
A tandemly repeated unit of 6553 bp containing a copy of the four core histone genes H2B, H2A, H3, and H4, and also a 5S rRNA gene, was amplified by PCR from genomic DNA of the isopod crustacean Asellus aquaticus. The linkage between 5S rRNA genes and histone genes has been so far observed in only one other organism, the anostrac crustacean Artemia salina. The gene cluster was cloned and sequenced. The histone genes, in their 3' flanking region, have the interesting feature of possessing two different mRNA termination signals, the stem-loop structure and the AATAAA sequence. A part of the PCR product was used as a probe in FISH experiments to locate the gene cluster on an inter-individually variable number of chromosomes from 6 to 12 per diploid cell, always in a terminal position and never associated with the heterochromatic areas. Fluorescence in situ hybridization (FISH) was also performed on preparations of released chromatin and the reiteration level of the gene cluster was determined as approximately 200-300 copies per haploid genome.
Barzotti
R
R
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italia.
Pelliccia
F
F
Bucciarelli
E
E
Rocchi
A
A
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003445:Crustacea / Q000235:genetics*; D006657:Histones / Q000235:genetics; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D017423:Sequence Analysis, RNA; D020080:Tandem Repeat Sequences
D006657:Histones; D012341:RNA, Ribosomal, 5S