Isolation, identification and determination of a magenta subsidiary colour in food blue no. 1 (brilliant blue FCF).
A magenta subsidiary colour was isolated from commercial Food Blue No. 1 (B-1; Brilliant Blue FCF). The absorption maximum for this subsidiary colour at 580 nm is outside of the range of 614-628 nm found for other subsidiary colours and m,m-B-1. On the basis of MS and NMR analyses, the structure of the subsidiary colour was elucidated as the disodium salt of 2-[[4-[N-ethyl-N-(3-sulphophenylmethyl)amino]phenyl][4-oxo- 2,5-cyclohexadienylidene]methyl]benzenesulphonic acid. HPLC analyses revealed that 24 batches of commercial Food Blue No. 1 (three manufacturers) contain 0.1-0.8% (average: 0.5%) of the magenta subsidiary colour.
Kusaka
T
T
College of Bioresource Sciences, Nihon University, Tokyo, Japan.
Matsufuji
H
H
Chino
M
M
Kato
Y
Y
Nakamura
M
M
Goda
Y
Y
Toyoda
M
M
Takeda
M
M
D001557:Benzenesulfonates / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013054:Spectrophotometry, Atomic; D014320:Triphenylmethyl Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D001557:Benzenesulfonates; D014320:Triphenylmethyl Compounds; C006796:brilliant blue