title: Dot assay for determining adhesive interactions between yeasts and bacteria under controlled hydrodynamic conditions.
abstract: Candida belongs to the normal human microflora and are found adhering to a number of human body tissues as well as to a variety of biomaterials implants. Often, yeasts adhere in association with bacteria, but to date there is no definitive assay to investigate adhesive interactions between yeasts and bacteria adhering on surfaces. Although we recently described the use of a parallel plate flow chamber to this purpose [Millsap, K.W., Bos, R., Van der Mei, H. C., Busscher, H.J., 1998. Adhesive interactions between medically important yeasts and bacteria. FEMS Microbiol. Rev. 21, 321-336], the method was slow and evaluation of a large number of strains showed major biological variation between experiments. Here, we describe a new assay for the simultaneous determination of the adhesive interactions between yeasts and different bacterial strains on a surface under controlled hydrodynamic conditions. On an acrylic surface, the presence of adhering bacteria suppressed adhesion of Candida albicans ATCC 10261 to various degrees, depending on the bacterial strain involved. Suppression of C. albicans ATCC 10261 adhesion was strongest by Actinomyces naeslundii T14V-J1, while adhering Streptococcus gordonii NCTC 7869 caused the weakest suppression of yeast adhesion. When adhering yeasts and bacteria were challenged with the high detachment force of a passing liquid-air interface, the majority of the yeasts detached, while C. albicans adhering on the control, bare polymethylmethacrylate surface formed aggregates. Summarizing, this study presents a new method to determine suggested adhesive interactions between yeasts and adhering bacteria under controlled hydrodynamic conditions. However, the results seem to indicate that these adhesive interactions may well not exist, but that instead different bacterial strains have varying abilities to discourage yeast adhesion.
authors.affiliation: Department of Biomedical Engineering, University of Groningen, Antonius Deusingloan 1, 9713 AV, Groningen, The Netherlands.
authors.forename: K W
authors.initials: KW
authors.lastname: Millsap
authors.forename: R
authors.initials: R
authors.lastname: Bos
authors.forename: H C
authors.initials: HC
authors.lastname: Van Der Mei
authors.forename: H J
authors.initials: HJ
authors.lastname: Busscher
mesh_terms: D000190:Actinomyces / Q000502:physiology; D001422:Bacterial Adhesion; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion; D008828:Microbiological Techniques; D019904:Polymethyl Methacrylate; D013211:Staphylococcus aureus / Q000502:physiology; D013291:Streptococcus / Q000502:physiology
chemical_list: D019904:Polymethyl Methacrylate