title: Effect of oral glucose on leucine turnover in human subjects at rest and during exercise at two levels of dietary protein.
abstract: The aim of this study was to determine the effect of glucose supplementation on leucine turnover during and after exercise and whether variation in the previous dietary protein content modulated this effect. Postabsorptive subjects received a primed constant [1-13C, 15N]leucine infusion for 6 h, after previous consumption of a high (1.8 g kg-1 day-1, HP, n = 16) or low (0.7 g kg-1 day-1, LP, n = 16) protein diet for 7 days. The subjects were studied at rest; during 2 h of exercise, during which half of the subjects from each dietary protocol received 0.75 g kg-1 h-1 glucose (HP + G, LP + G) and the other half received water (HP + W, LP + W); then again for 2 h of rest. Glucose supplementation suppressed leucine oxidation (P < 0.01) by 20% in subjects consuming the high protein diet (58.2 +/- 2.8 micromol kg-1 h-1, HP + G; 72.4 +/- 3.9 micromol kg-1 h-1, HP + W) but not the low protein diet (51.1 +/- 5.9 micromol kg-1 h-1, LP + G; 51.7 +/- 5.5 micromol kg-1 h-1, LP + W), with no difference in skeletal muscle branched-chain 2-oxo acid dehydrogenase (BCOADH) activity between groups. Glucose supplementation did not alter the rate of whole-body protein synthesis or breakdown. The sparing effect of glucose on leucine oxidation appears only to occur if previous protein intake was high. It was not mediated by a suppression of BCOADH fractional activity but may be due to reduced substrate availability.
authors.affiliation: Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, Scotland, UK. bowteljl@sbu.ac.uk
authors.forename: J L
authors.initials: JL
authors.lastname: Bowtell
authors.forename: G P
authors.initials: GP
authors.lastname: Leese
authors.forename: K
authors.initials: K
authors.lastname: Smith
authors.forename: P W
authors.initials: PW
authors.lastname: Watt
authors.forename: A
authors.initials: A
authors.lastname: Nevill
authors.forename: O
authors.initials: O
authors.lastname: Rooyackers
authors.forename: A J
authors.initials: AJ
authors.lastname: Wagenmakers
authors.forename: M J
authors.initials: MJ
authors.lastname: Rennie
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000284:Administration, Oral; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D015444:Exercise; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000097:blood; D007656:Ketoglutaric Acids / Q000378:metabolism; D007658:Ketone Oxidoreductases / Q000378:metabolism; D007930:Leucine / Q000097:blood / Q000378:metabolism*; D008297:Male; D009097:Multienzyme Complexes / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism; D009587:Nitrogen Isotopes; D014176:Protein Biosynthesis; D012146:Rest
chemical_list: D001786:Blood Glucose; D002247:Carbon Isotopes; D004044:Dietary Proteins; D007328:Insulin; D007656:Ketoglutaric Acids; D009097:Multienzyme Complexes; D009587:Nitrogen Isotopes; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D007930:Leucine; D005947:Glucose