title: Conformational changes in the cytochrome b6f complex induced by inhibitor binding.
abstract: Binding of stigmatellin, an inhibitor of the Q(o) site of the bc-type complexes, has been shown to induce large conformational changes of the Rieske protein in the respiratory bc(1) complex (Kim, H., Xia, D., Yu, C. A., Xia, J. Z., Kachurin, A. M., Zhang, L., Yu, L., and Deisenhofer, J. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 8026-8033; Iwata, S., Lee, J. W., Okada, K., Lee, J. K., Iwata, M., Rasmussen, B., Link, T. A., Ramaswamy, S., and Jap, B. K. (1998) Science 281, 64-71; Zhang, Z., Huang, L., Shulmeister, V. M., Chi, Y. I., Kim, K. K., Hung, L. W., Crofts, A. R., Berry, E. A., and Kim, S. H. (1998) Nature 392, 677-684). Such a movement seems necessary to shuttle electrons from the membrane-soluble quinol to the extramembrane heme of cytochrome c(1). To see whether similar changes occur in the related photosynthetic b(6)f complex, we have studied the effect of the binding of stigmatellin to the eukaryotic b(6)f complex by electron crystallography. Comparison of projection maps of thin three-dimensional crystals prepared with or without stigmatellin, and either negatively stained or embedded in glucose, reveals a similar type of movement to that observed in the bc(1) complex and suggests also the occurrence of conformational changes in the transmembrane region.
authors.affiliation: Max-Planck-Institute of Biophysics, Department of Structural Biology, Heinrich-Hoffmann-Strasse 7, D-60528 Frankfurt am Main, Germany. breyton@mpibp-frankfurt.mpg.de
authors.forename: C
authors.initials: C
authors.lastname: Breyton
mesh_terms: D000818:Animals; D016825:Chlamydomonas reinhardtii; D003573:Cytochrome b Group / Q000037:antagonists & inhibitors / Q000737:chemistry*; D045346:Cytochrome b6f Complex; D004791:Enzyme Inhibitors / Q000737:chemistry; D011090:Polyenes / Q000737:chemistry; D011487:Protein Conformation
chemical_list: D003573:Cytochrome b Group; D004791:Enzyme Inhibitors; D011090:Polyenes; D045346:Cytochrome b6f Complex; C041573:stigmatellin