title: IL-8 producing cells in patients with Behçet's disease.
abstract: OBJECTIVE
  IL-8 is thought to be the principal chemokine responsible for neutrophil activation and tissue infiltration in patients with Behçet's disease (BD). In various studies serum levels of IL-8 were reported to be increased.
  
  
  METHODS
  IL-8 mRNA was purified from both peripheral whole blood samples and separated lymphocytes, granulocytes and monocytes of patients with BD and compared to that from healthy (HC) and disease controls. IL-8 sequences were revealed by PCR amplification using appropriate sequence-specific primers. mRNA levels were determined semi-quantitatively using an image analyser in comparison with beta-actin.
  
  
  RESULTS
  Although the differences did not reach statistical significance, BD patients tended to have higher IL-8 mRNA levels compared to HC in whole blood samples (2.0 +/- 1.4 vs 1.5 +/- 1.2) as well as in their lymphocytes (2.7 +/- 1.6 vs 1.5 +/- 0.9). No differences were observed between BD and HC in monocyte or granulocyte IL-8 mRNA levels.
  
  
  CONCLUSIONS
  Our results suggest that the cellular source of IL-8 is diverse in BD with a possible major contribution by lymphocytes.
authors.affiliation: Division of Hematology and Immunology, Faculty of Medicine, Marmara University, Istanbul, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Mantas
authors.forename: H
authors.initials: H
authors.lastname: Direskeneli
authors.forename: D
authors.initials: D
authors.lastname: Oz
authors.forename: S
authors.initials: S
authors.lastname: Yavuz
authors.forename: T
authors.initials: T
authors.lastname: Akoglu
mesh_terms: D000199:Actins / Q000097:blood / Q000235:genetics; D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood; D001528:Behcet Syndrome / Q000097:blood*; D002469:Cell Separation; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000097:blood* / Q000235:genetics; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D018805:Sepsis / Q000097:blood
chemical_list: D000199:Actins; D017931:DNA Primers; D016209:Interleukin-8; D012333:RNA, Messenger