Formation of surface tubules and fluorescent foci in Arabidopsis thaliana protoplasts expressing a fusion between the green fluorescent protein and the cauliflower mosaic virus movement protein.
The movement protein (MP) of cauliflower mosaic virus (CaMV) is a multifunctional protein that potentiates the cell-to-cell and long distance movement of the virus. Functional domains in the CaMV MP were determined by analyzing deletions in green fluorescent protein (GFP)-MP fusions transfected into Arabidopsis thaliana leaf protoplasts. GFP-MP accumulated at fluorescent foci at the cell periphery and in tubular structures extending from the protoplast surface. A region located near the center of MP was required for targeting GFP-MP to foci, whereas a larger region extending nearly to the N-terminus was needed for tubule formation. Cytoskeletal assembly inhibitors did not disrupt tubule formation or the accumulation of GFP-MP at foci, but brefeldin A, which disrupts the plant cell endomembrane system, did interfere with the formation of tubules but not foci.
Huang
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Boyce Thompson Institute, Cornell University, Ithaca, New York 14853, USA.
Han
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Howell
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SH
D000998:Antiviral Agents / Q000494:pharmacology; D017360:Arabidopsis / Q000254:growth & development* / Q000648:ultrastructure; D020126:Brefeldin A / Q000494:pharmacology; D017796:Caulimovirus; D003599:Cytoskeleton / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D053830:Plant Viral Movement Proteins; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D000998:Antiviral Agents; D008164:Luminescent Proteins; D053830:Plant Viral Movement Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D020126:Brefeldin A