The effects of contignasterol (IZP-94,005) on allergen-induced plasma protein exudation in the tracheobronchial airways of sensitized guinea-pigs in vivo.
OBJECTIVE
To examine contignasterol (marine-derived) on allergen-induced plasma exudation in the tracheobronchial airways.


METHODS
English shorthair guinea-pigs actively sensitized to ovalbumin.


METHODS
21 to 36 days after sensitization, contignasterol, budesonide or nedocromil and then ovalbumin (12 pmol) were superfused onto the tracheal mucosa (i. t.) of anaesthetized animals.


METHODS
Tracer (125I-human serum albumin) was measured in tracheobronchial lavage.


RESULTS
Thirty min after administration of contignasterol (100 and 200, but not 0.2 or 2.0 microg/kg), budesonide or nedocromil (200 microg/kg), the ovalbumin response (plasma exudate, microl) was significantly inhibited. The response was also inhibited 5 min after 200 microg/kg nedocromil but not after contignasterol or budesonide.


CONCLUSIONS
Contignasterol was topically active and showed anti-inflammatory effects in the tracheobronchial airways in that it inhibited allergen-induced plasma protein exudation.
Coulson
F R
FR
Department of Physiology and Pharmacology, The University of Queensland, Brisbane, Australia.
O'Donnell
S R
SR
D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage*; D001798:Blood Proteins / Q000378:metabolism*; D001980:Bronchi / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D019819:Budesonide / Q000008:administration & dosage; D005122:Exudates and Transudates / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007442:Intubation, Intratracheal; D008297:Male; D017835:Nedocromil / Q000008:administration & dosage; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D013261:Sterols / Q000008:administration & dosage*; D014132:Trachea / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D000485:Allergens; D018927:Anti-Asthmatic Agents; D001798:Blood Proteins; D013261:Sterols; D017835:Nedocromil; C091550:contignasterol; D019819:Budesonide; D010047:Ovalbumin