The effects of attachment factors on initial attachment of human periodontal ligament fibroblasts on different root surfaces: a light and scanning electron microscopic study.
This study was aimed to test the effect of fibronectin (FN), vitronectin (VN) and a fibronectin analog (fibronectin-like engineered protein) on the attachment of periodontal ligament cells to mechanically-treated and mechanically non-treated periodontally involved and non-diseased root surfaces in vitro. Periodontal ligament fibroblasts were incubated with a total of 44 periodontally diseased and non-diseased root slices which had been treated in the following manner: 1) FN applied to mechanically-treated and non-treated root slices, 2) VN applied to mechanically-treated and non-treated root slices, 3) FN-like engineered protein applied to mechanically-treated and non-treated root slices, and 4) mechanically-treated and non-treated root slices. After the 1 hour incubation period in a humidified atmosphere of 95% air and 5% CO2 at 37 degrees C, the adherence of the fibroblasts was determined using light microscopy with an ocular grid system and orientation was evaluated by scanning electron microscopy. The results indicated that the number of attached cells to non-diseased cementum sites was significantly greater than the number of attached cells to diseased cementum sites for all of the groups (p < 0.05). Likewise, the number of attached cells to mechanically-treated diseased cementum sites was significantly greater than the number of attached cells to mechanically-non-treated diseased cementum sites (p < 0.05). Our findings suggest that these attachment factors cannot promote cell attachment to different cementum sites.
Tüter
G
G
University of Gazi, Faculty of Dentistry, Department of Periodontology, Ankara, Turkey.
Yalim
M
M
Gürhan
I
I
Baloş
K
K
D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003739:Dental Cementum / Q000187:drug effects / Q000502:physiology* / Q000503:physiopathology / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D017622:Periodontal Attachment Loss / Q000503:physiopathology*; D010513:Periodontal Ligament / Q000502:physiology* / Q000503:physiopathology / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000494:pharmacology; D019096:Vitronectin / Q000494:pharmacology
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D011994:Recombinant Proteins; D019096:Vitronectin