Practice of a testing bench to study the effects of cyclic stretching on osteoblast-orthopaedic ceramic interactions.
A new experimental method has been used to study the behaviour of human osteoblasts cultured on bioceramics subjected to mechanical strains. The ceramics were alumina, hydroxyapatite (HA) and a duplex system composed of hydroxyapatite-covered alumina. The system applied 400 microdeformations for a 6-h period with a cycle frequency of 0.5 Hz to osteoblasts growing on ceramic-covered disks. The effects of strains on short-term cell viability, cell growth, alkaline phosphatase (ALP) activity, and collagen biosynthesis were assessed. When possible, the parameters (lactate dehydrogenase) were studied along the experiment in samples of the culture medium, in the other cases by comparison of stretched and unstretched cultures on the same ceramics with the same cell line. In relationship with the coating, mechanical strains resulted in a decrease in DNA corresponding to cell number, an LDH release during straining, an unchanged (alumina) or decreased (HA and duplex) ALP activity, a decrease (HA and duplex) of collagen and total protein synthesis or an increase of it (alumina). The stress-producing device and its associated protocol are shown to be suitable for investigating the behaviour of cells, cultured on biomaterials subjected to mechanical strain.
Labat
B
B
Laboratoire de Biochimie, Faculté de Médecine, St. Etienne, France.
Chepda
T
T
Frey
J
J
Rieu
J
J
Aurelle
J L
JL
Douet
M
M
Alexandre
C
C
Chamson
A
A
D000469:Alkaline Phosphatase / Q000032:analysis; D000497:Alloys; D000537:Aluminum Oxide; D001672:Biocompatible Materials; D015415:Biomarkers; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics; D020099:Coated Materials, Biocompatible; D017077:Culture Media, Conditioned; D017886:Durapatite; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D006622:Hip Prosthesis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008422:Materials Testing / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D016062:Porosity; D014176:Protein Biosynthesis; D013314:Stress, Mechanical; D014025:Titanium
D000497:Alloys; D001672:Biocompatible Materials; D015415:Biomarkers; D020099:Coated Materials, Biocompatible; D017077:Culture Media, Conditioned; C031462:titanium alloy (TiAl6V4); D017886:Durapatite; D014025:Titanium; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D000537:Aluminum Oxide