Automated parallel DNA sequencing on multiple channel microchips.
We report automated DNA sequencing in 16-channel microchips. A microchip prefilled with sieving matrix is aligned on a heating plate affixed to a movable platform. Samples are loaded into sample reservoirs by using an eight-tip pipetting device, and the chip is docked with an array of electrodes in the focal plane of a four-color scanning detection system. Under computer control, high voltage is applied to the appropriate reservoirs in a programmed sequence that injects and separates the DNA samples. An integrated four-color confocal fluorescent detector automatically scans all 16 channels. The system routinely yields more than 450 bases in 15 min in all 16 channels. In the best case using an automated base-calling program, 543 bases have been called at an accuracy of >99%. Separations, including automated chip loading and sample injection, normally are completed in less than 18 min. The advantages of DNA sequencing on capillary electrophoresis chips include uniform signal intensity and tolerance of high DNA template concentration. To understand the fundamentals of these unique features we developed a theoretical treatment of cross-channel chip injection that we call the differential concentration effect. We present experimental evidence consistent with the predictions of the theory.
Liu
S
S
Molecular Dynamics/Amersham Pharmacia Biotech, Sunnyvale, CA 94086, USA. sharong.liu@am.apbiotech.com
Ren
H
H
Gao
Q
Q
Roach
D J
DJ
Loder
R T
RT
Armstrong
T M
TM
Mao
Q
Q
Blaga
I
I
Barker
D L
DL
Jovanovich
S B
SB
D001331:Automation / Q000295:instrumentation / Q000379:methods; D001483:Base Sequence; D004867:Equipment Design; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods*; D013698:Templates, Genetic