[Streptomyces sp. Z-11-6--a new producer of extracellular L-glutamate oxidase].
Glutamate oxidase activity was studied in 1254 Streptomyces strains isolated from the zonal soils of various regions of Russia and other countries. Seven strains proved to be producers of extracellular L-glutamate oxidase. The most active producer strain was identified, and the conditions of enzyme biosynthesis were optimized. A multistep-mutagenesis and selection procedure allowed a genetically stable strain, Streptomyces sp. Z-11-6, to be obtained, whose glutamate oxidase activity was 40 times higher than that of the original natural isolate.
Sukhacheva
M V
MV
Department of Microbiology, Biological Faculty, Moscow State University, Vorob'evy gory, Russia.
Netrusov
A I
AI
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis* / Q000378:metabolism; D016296:Mutagenesis; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D000594:Amino Acid Oxidoreductases; C023061:L-glutamate oxidase