Some general methods of preparing affinity columns.
Some general methods of covalent coupling of nucleotides, especially derivatized nucleotides, polynucleotides and cofactors to insoluble polymers are described in this paper. Wherever necessary individual methods also carry some information on the binding of enzymes to the same polymers to serve as a guide to the efficiency of the coupling methods.
Sundaram
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D002245:Carbon Dioxide; D002266:Carboxymethylcellulose Sodium; D002482:Cellulose / Q000031:analogs & derivatives; D002846:Chromatography, Affinity / Q000379:methods*; D003488:Cyanogen Bromide; D004247:DNA / Q000737:chemistry; D005976:Glutaral; D009711:Nucleotides / Q000737:chemistry; D009757:Nylons; D011108:Polymers; D011119:Polynucleotides / Q000737:chemistry; D012685:Sepharose / Q000737:chemistry; D012995:Solubility
D009711:Nucleotides; D009757:Nylons; D011108:Polymers; D011119:Polynucleotides; D002245:Carbon Dioxide; D002482:Cellulose; D004247:DNA; D012685:Sepharose; C010021:aminoethyl cellulose; D002266:Carboxymethylcellulose Sodium; D003488:Cyanogen Bromide; D005976:Glutaral