Coexistence of intestinal trefoil factor (hITF) and oxytocin in magnocellular neurons in the human hypothalamus.
Human intestinal trefoil factor hITF, a polypeptide of the P-domain family, was found to occur in hypothalamic neurons. With combined immunofluorescence and immunoperoxidase technique we investigated the coexistence of hITF with the neurohypophysial peptide oxytocin and the associated neurophysin I in sections of the human hypothalamus. In the supraoptic nucleus, 39.2% of magnocellular oxytocinergic perikarya show hITF immunoreactivity. A similar distribution was observed in perivascular hypothalamic oxytocinergic neurons, whereas in the paraventricular nucleus, 99% of the oxytocinergic neurons show hITF coexpression. In the periventricular nucleus (PEV), single, scattered neurons with both immunoreactivities occur. Our findings indicate that hITF and oxytocin are coexpressed in a portion of the magnocellular neurons in the human hypothalamus, and that hITF is among the neurohypophysial peptides.
Griepentrog
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Institut für Anatomie, Anatomie II, Friedrich-Schiller-Universität Jena, Germany.
Bauer
M
M
Hornstein
C
C
Sauer
H
H
Jirikowski
G F
GF
D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005455:Fluorescent Antibody Technique; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009077:Mucins; D009124:Muscle Proteins; D009474:Neurons / Q000737:chemistry*; D009479:Neuropeptides; D010121:Oxytocin / Q000032:analysis*; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry* / Q000166:cytology; D010455:Peptides / Q000032:analysis*; D013495:Supraoptic Nucleus / Q000737:chemistry* / Q000166:cytology; D016707:Tissue Fixation; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
D006133:Growth Substances; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; C099037:TFF3 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D010121:Oxytocin