Cloning of the cDNA and localization of the gene encoding human NRBP, a ubiquitously expressed, multidomain putative adapter protein.
Adapter proteins modulate multiple signaling pathways by regulating the aggregation of other factors into signaling complexes. Here we have identified a novel human cDNA encoding NRBP, a multidomain putative adapter protein containing (i) two putative nuclear receptor binding motifs (LXXLL), (ii) a putative binding domain for Src homology-2 (SH2) domain containing proteins, (iii) a kinase-like domain, (iv) a bipartite nuclear localization signal, and (v) three sequences rich in glutamic acid, serine, proline, and threonine (PEST) residues. The NRBP mRNA transcript, of approximately 2.4 kb, was ubiquitously expressed in a wide range of normal human tissues and 15 human tumor cell lines. The NRBP cDNA is predicted to encode a polypeptide of 535 amino acids with a molecular mass of 59.8 kDa. Translation of NRBP mRNA in vitro reveals three translation products of 60, 51, and 43 kDa, suggesting that translation of NRBP may initiate at multiple sites. The NRBP gene was localized to human chromosome 2p23, near the location of the NCOA1 gene encoding the nuclear receptor coactivator, steroid receptor coactivator-1 (SRC-1). The features of NRBP predict a function as an adapter protein potentially linking signaling pathways involving nuclear receptors and SH2 domain containing proteins.
Hooper
J D
JD
Cellular Oncology Laboratory, University of Queensland, Brisbane, Queensland, 4029, Australia.
Baker
E
E
Ogbourne
S M
SM
Sutherland
G R
GR
Antalis
T M
TM
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D020816:Amino Acid Motifs; D001483:Base Sequence; D001665:Binding Sites; D001685:Biological Factors / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010770:Phosphotransferases / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects; D011494:Protein Kinases; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014018:Tissue Distribution; D033921:Vesicular Transport Proteins; D018909:src Homology Domains / Q000235:genetics
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D001685:Biological Factors; D018076:DNA, Complementary; D046911:Macromolecular Substances; C410809:NRBP1 protein, human; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D033921:Vesicular Transport Proteins; D010770:Phosphotransferases; D011494:Protein Kinases