title: Computer-based three-dimensional visualization of developmental gene expression.
abstract: A broad understanding of the relationship between gene activation, pattern formation and morphogenesis will require adequate tools for three-dimensional and, perhaps four-dimensional, representation and analysis of molecular developmental processes. We present a novel, computer-based method for the 3D visualization of embryonic gene expression and morphological structures from serial sections. The information from these automatically aligned 3D reconstructions exceeds that from single-section and whole-mount visualizations of in situ hybridizations. In addition, these 3D models of gene-expression patterns can become a central component of a future developmental database designed for the collection and presentation of digitized, morphological and gene-expression data. This work is accompanied by a web site (http://www.univie.ac.at/GeneEMAC).
authors.affiliation: Integrative Morphology Group, Department of Anatomy, University of Vienna, Vienna, Austria. johannes.streicher@univie.ac.at
authors.forename: J
authors.initials: J
authors.lastname: Streicher
authors.forename: M A
authors.initials: MA
authors.lastname: Donat
authors.forename: B
authors.initials: B
authors.lastname: Strauss
authors.forename: R
authors.initials: R
authors.lastname: Spörle
authors.forename: K
authors.initials: K
authors.lastname: Schughart
authors.forename: G B
authors.initials: GB
authors.lastname: Müller
mesh_terms: D019412:Anatomy, Cross-Sectional / Q000379:methods; D000818:Animals; D001331:Automation; D003198:Computer Simulation; D016208:Databases, Factual; D004622:Embryo, Mammalian / Q000033:anatomy & histology* / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers / Q000235:genetics; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods; D020407:Internet; D051379:Mice; D009024:Morphogenesis / Q000235:genetics; D009928:Organ Specificity; D010363:Pattern Recognition, Automated; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012984:Software; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D005819:Genetic Markers