title: Monoclonality of intraepidermal T lymphocytes in early mycosis fungoides detected by molecular analysis after laser-beam-based microdissection.
abstract: The identification of neoplastic lymphocytes in early lesions of mycosis fungoides is difficult because of the scarcity of the infiltrate and the presence of reactive T lymphocytes admixed with neoplastic cells. Molecular analysis of the T cell receptor gene rearrangement using the polymerase chain reaction technique demonstrates monoclonality only in a proportion of these cases. The exact location of the malignant clone is unknown, and at present it is not clear whether neoplastic cells in early lesions reside within the epidermis, the superficial dermis, or both. We analyzed skin lesions from five patients with early mycosis fungoides using the polymerase chain reaction technique after microdissection of the specimens. In each case the epidermis was separated from the dermis using a laser-beam microdissection technique. Three samples were prepared from each lesion: one containing only the epidermis, one only the superficial dermis, and one the entire specimen. A distinct band could be observed in the epidermal sample in four cases, indicating the presence of an intraepidermal monoclonal population of T lymphocytes. The dermal sample revealed a monoclonal pattern in two cases (both of them showing clonality also within the epidermis). Analysis of the entire specimen revealed a monoclonal pattern only in two cases. Our results demonstrate that intraepidermal lymphocytes in early mycosis fungoides often show a monoclonal pattern of T cell receptor gene rearrangement. Microdissection of biopsy specimens may enhance the sensitivity of the polymerase chain reaction technique.
authors.affiliation: Department of Dermatology, University of Graz, Austria. lorenzo.cerroni@kfunigraz.ac.at
authors.forename: L
authors.initials: L
authors.lastname: Cerroni
authors.forename: E
authors.initials: E
authors.lastname: Arzberger
authors.forename: M
authors.initials: M
authors.lastname: Ardigò
authors.forename: B
authors.initials: B
authors.lastname: Pütz
authors.forename: H
authors.initials: H
authors.lastname: Kerl
mesh_terms: D000199:Actins / Q000235:genetics; D000328:Adult; D000368:Aged; D001706:Biopsy; D003001:Cloning, Molecular; D004210:Dissection / Q000379:methods*; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D007834:Lasers; D008297:Male; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000473:pathology*; D016133:Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis / Q000235:genetics; D012867:Skin / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000199:Actins; D016692:Receptors, Antigen, T-Cell, gamma-delta