Neonatal capsaicin treatment prevents the normal postnatal withdrawal of A fibres from lamina II without affecting fos responses to innocuous peripheral stimulation.
The development of spinal cord sensory pathways has been investigated in postnatal day (P) 21 rat pups following neonatal capsaicin treatment. Capsaicin-induced destruction of C fibres was confirmed by 62% loss of Isolectin B4 (IB4)-binding and an 86% loss of calcitonin gene-related peptide (CGRP)-immunoreactive small diameter dorsal root ganglion cells. Neonatal capsaicin treatment prevented the normal withdrawal of choleragenoid-horseradish peroxidase (B-HRP)-labelled A fibres from lamina II (substantia gelatinosa) to deeper laminae postnatally. A fibre terminals projected more dorsally, extending into 43% of lamina II compared to vehicle-treated littermates. A small cell loss in, and/or shrinkage of, substantia gelatinosa cannot account for this. These support the concept of a competitive interaction between A and C fibre afferents to establish final terminal fields. However the continued exuberant A fibre termination in capsaicin-treated rats did not lead to continued c-fos induction in the superficial dorsal horn by innocuous stimulation. In normal development, exuberant A fibre terminals coincide with c-fos activation in lamina II by innocuous skin stimulation [23]. Despite the continued presence of exuberant A fibre terminals, c-fos was not induced by innocuous peripheral stimulation in P21 capsaicin-treated rats implying that these superficial terminals do not activate lamina II neurons in the same way as in the neonate.
Torsney
C
C
Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, UK. c.tornsey@ucl.ac.uk
Meredith-Middleton
J
J
Fitzgerald
M
M
D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002211:Capsaicin / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology / Q000254:growth & development*; D009413:Nerve Fibers, Myelinated / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D009475:Neurons, Afferent / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D010812:Physical Stimulation; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*; D051381:Rats; D013376:Substantia Gelatinosa / Q000166:cytology / Q000254:growth & development*
D015415:Biomarkers; D016760:Proto-Oncogene Proteins c-fos; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin