title: Evaluation of a new system, VITEK 2, for identification and antimicrobial susceptibility testing of enterococci.
abstract: We evaluated the new automated VITEK 2 system (bioMérieux) for the identification and antimicrobial susceptibility testing of enterococci. The results obtained with the VITEK 2 system were compared to those obtained by reference methods: standard identification by the scheme of Facklam and Sahm [R. R. Facklam and D. F. Sahm, p. 308-314, in P. R. Murray et al., ed., Manual of Clinical Microbiology, 6th ed., 1995] and with the API 20 STREP system and, for antimicrobial susceptibility testing, broth microdilution and agar dilution methods by the procedures of the National Committee for Clinical Laboratory Standards. The presence of vanA and vanB genes was determined by PCR. A total of 150 clinical isolates were studied, corresponding to 60 Enterococcus faecalis, 55 Enterococcus faecium, 26 Enterococcus gallinarum, 5 Enterococcus avium, 2 Enterococcus durans, and 2 Enterococcus raffinosus isolates. Among those isolates, 131 (87%) were correctly identified to the species level with the VITEK 2 system. Approximately half of the misidentifications were for E. faecium with low-level resistance to vancomycin, identified as E. gallinarum or E. casseliflavus; however, a motility test solved the discrepancies and increased the agreement to 94%. Among the strains studied, 66% were vancomycin resistant (57 VanA, 16 VanB, and 26 VanC strains), 23% were ampicillin resistant (MICs, >/=16 microgram/ml), 31% were high-level gentamicin resistant, and 45% were high-level streptomycin resistant. Percentages of agreement for susceptibility and resistance to ampicillin, vancomycin, and teicoplanin and for high-level gentamicin resistance and high-level streptomycin resistance were 93, 95, 97, 97, and 96%, respectively. The accuracy of identification and antimicrobial susceptibility testing of enterococci with the VITEK 2 system, together with the significant reduction in handling time, will have a positive impact on the work flow of the clinical microbiology laboratory.
authors.affiliation: Servicio de Microbiología, Hospital General Universitario "Gregorio Marañón," Madrid, Spain. fgarrote@efd.net
authors.forename: F
authors.initials: F
authors.lastname: Garcia-Garrote
authors.forename: E
authors.initials: E
authors.lastname: Cercenado
authors.forename: E
authors.initials: E
authors.lastname: Bouza
mesh_terms: D015373:Bacterial Typing Techniques / Q000295:instrumentation* / Q000592:standards; D004352:Drug Resistance, Microbial; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects*; D005069:Evaluation Studies as Topic; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000592:standards; D013997:Time Factors