title: Comparison of outer membrane protein genes omp and pmp in the whole genome sequences of Chlamydia pneumoniae isolates from Japan and the United States.
abstract: Chlamydia pneumoniae is a widespread pathogen of the respiratory tract that is also associated with atherosclerosis. The whole genome sequence was determined for a Japanese isolate, C. pneumoniae strain J138. The sequence predicted a variety of genes encoding outer membrane proteins (OMPs) including ompA and porB, another 10 predicted omp genes, and 27 pmp genes. All were detected in the whole genome sequence of strain CWL029, a strain isolated and sequenced in the United States. A comparative study of the OMPs of the two strains revealed a nucleotide sequence identity of 89.6%-100% (deduced amino acid sequence identity, 71.1%-100%). The overall genomic organization and location of genes are identical in both strains. Thus, a few unique sequences of the OMPs may be essential for specific attributes that define the differential biology of two C. pneumoniae strains.
authors.affiliation: Dept. of Microbiology, Yamaguchi University School of Medicine, Ube, Yamaguchi 755-8505, Japan. mshirai@po.cc.yamaguchi-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Shirai
authors.forename: H
authors.initials: H
authors.lastname: Hirakawa
authors.forename: K
authors.initials: K
authors.lastname: Ouchi
authors.forename: M
authors.initials: M
authors.lastname: Tabuchi
authors.forename: F
authors.initials: F
authors.lastname: Kishi
authors.forename: M
authors.initials: M
authors.lastname: Kimoto
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: J
authors.initials: J
authors.lastname: Nishida
authors.forename: K
authors.initials: K
authors.lastname: Shibata
authors.forename: R
authors.initials: R
authors.lastname: Fujinaga
authors.forename: H
authors.initials: H
authors.lastname: Yoneda
authors.forename: H
authors.initials: H
authors.lastname: Matsushima
authors.forename: C
authors.initials: C
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Furukawa
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: A
authors.initials: A
authors.lastname: Nakazawa
authors.forename: K
authors.initials: K
authors.lastname: Ishii
authors.forename: T
authors.initials: T
authors.lastname: Shiba
authors.forename: M
authors.initials: M
authors.lastname: Hattori
authors.forename: S
authors.initials: S
authors.lastname: Kuhara
authors.forename: T
authors.initials: T
authors.lastname: Nakazawa
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002690:Chlamydia Infections / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000235:genetics* / Q000302:isolation & purification; D016680:Genome, Bacterial; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D014481:United States
chemical_list: D001425:Bacterial Outer Membrane Proteins