Determination of beryllium in a stream sediment by high-performance chelation ion chromatography.
High-performance chelation ion chromatography (HPCIC), involving a chelating silica substrate bonded with aminomethylphosphonic acid, has been developed as a novel technique for the quantitative determination of beryllium in complex matrices. An isocratic separation method, using an eluent containing 1 M KNO3, 0.5 M HNO3 and 0.08 M ascorbic acid, allowed the Be2+ to elute away from the sample matrix peak in under 6 min in a sample containing in excess of 800 mg l(-1) matrix metals. A detection limit of 35 microg l(-1) Be(II) was found using a post-column reaction involving Chrome Azurol S (CAS), 1 M hexamine and 10 mM EDTA buffered at pH 6. The standard addition curve gave excellent linearity (R2>0.999). The procedure was applied to the determination of trace beryllium in a certified sediment sample. The results obtained compared well with the certified value for beryllium.
Shaw
M J
MJ
Department of Environmental Sciences, University of Plymouth, Devon, UK.
Hill
S J
SJ
Jones
P
P
Nesterenko
P N
PN
D001608:Beryllium / Q000032:analysis*; D002274:Carcinogens, Environmental / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D009943:Organophosphorus Compounds / Q000737:chemistry; D009994:Osmolar Concentration; D012822:Silicon Dioxide / Q000737:chemistry; D013997:Time Factors; D014873:Water Pollutants / Q000032:analysis*
D002274:Carcinogens, Environmental; D009943:Organophosphorus Compounds; D014873:Water Pollutants; C036423:1-aminomethylphosphonic acid; D012822:Silicon Dioxide; D001608:Beryllium