title: Isolation of steroidal sapogenins implicated in experimentally induced cholangiopathy of sheep grazing Brachiaria decumbens in Brazil.
abstract: As part of an experimental study, crystal-associated cholangiopathy was induced in 9 sheep by grazing pure pastures of Brachiaria decumbens in Brazil. One of these sheep showed characteristic lesions of photosensitization. The analysis of the B decumbens samples by acidic hydrolysis followed by TLC and infrared spectrum revealed diosgenin as the principal sapogenin present in the plant. In the rumen contents samples from the B decumbens-grazing group were identified by TLC, 1H and 13C NMR and EIMS as epismilagenin, episarsasapogenin, and a mixture of smilagenin and sarsasapogenin. In the bile samples from the B decumbens-grazing group, TLC analysis demonstrated 2 compounds similar to epismilagenin and episarsasapogenin. However, by this same method, those compounds were not observed in the rumen contents and bile from 2 sheep which served as control animals. The P chartarum spore counts remained very low during the experimental period.
authors.affiliation: Department of Veterinary Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Cruz
authors.forename: D
authors.initials: D
authors.lastname: Driemeier
authors.forename: V S
authors.initials: VS
authors.lastname: Pires
authors.forename: E M
authors.initials: EM
authors.lastname: Colodel
authors.forename: A T
authors.initials: AT
authors.lastname: Taketa
authors.forename: E P
authors.initials: EP
authors.lastname: Schenkel
mesh_terms: D000818:Animals; D001203:Ascomycota / Q000254:growth & development; D001646:Bile / Q000737:chemistry; D001938:Brazil; D002761:Cholangitis / Q000139:chemically induced / Q000209:etiology / Q000662:veterinary*; D002855:Chromatography, Thin Layer / Q000662:veterinary; D015169:Colony Count, Microbial / Q000662:veterinary; D004144:Diosgenin / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D006651:Histocytochemistry; D008099:Liver / Q000473:pathology; D008107:Liver Diseases / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000662:veterinary; D010787:Photosensitivity Disorders / Q000139:chemically induced / Q000209:etiology / Q000662:veterinary*; D006109:Poaceae / Q000737:chemistry* / Q000382:microbiology / Q000633:toxicity; D012417:Rumen / Q000737:chemistry; D012502:Sapogenins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D012756:Sheep; D012757:Sheep Diseases / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D013055:Spectrophotometry, Infrared / Q000662:veterinary; D013150:Spirostans / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D012502:Sapogenins; D013150:Spirostans; C046297:sarsasapogenin; D004144:Diosgenin