Sympathetic activation of hepatic and splenic IL-1beta mRNA expression during oscillation stress in the rat.
Interleukin (IL)-1beta mRNA expression in the liver and spleen was examined after subjection to oscillation stress in the rat. Thirty-minute subjection to oscillation stress increased IL-1beta mRNA expression in the both organs. Prior treatment of rats with gadolinium chloride, which eliminates macrophages, prevented the stress-induced IL-1beta expression. Either adrenalectomy or treatment of guanethidine, a blocker of norepinephrine release in the sympathetic nerve endings, partially attenuated the stress-induced response, but the combined treatment completely blocked it. Injection of beta-adrenergic antagonist (propranolol) also suppressed the stress-induced response. These results suggest that oscillation stress induces IL-1beta mRNA expression in the liver and spleen, probably in Kupffer cells and splenic macrophages, and that stress-induced IL-1beta expression is elicited by catecholamines released from sympathetic nerve terminals and the adrenal gland.
Jung
B D
BD
Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kimura
K
K
Kitamura
H
H
Makondo
K
K
Kanehira
K
K
Saito
M
M
D000315:Adrenalectomy; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D005682:Gadolinium / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar / Q000378:metabolism*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism / Q000662:veterinary*
D000319:Adrenergic beta-Antagonists; D007375:Interleukin-1; D012333:RNA, Messenger; D011433:Propranolol; D005682:Gadolinium; C038958:gadolinium chloride; D009638:Norepinephrine