Kinetics of lymphocytic subsets in chicken tracheal lesions infected with infectious bronchitis virus.
The kinetics of T-cells (CD3 positive (+), CD4+ and CD8+ cells) and B-cells (IgG+, IgM+ and IgA+ cells) in chicken trachea were studied immunohistochemically and histopathologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 16 hr to 6 days post-inoculation (p.i.) with a peak on 4 days p.i. A few IgG+, IgM+ and IgA+ cells were detected in the submucosa from 8 hr p.i. Thereafter IgG+ and IgM+ cells were gradually increased in number, and dramatically increased from 3 days p.i., peaked on 4 days p.i., and gradually decreased after 5 days p.i. IgA+ cells were detected in a small number than IgG+ and IgM+ cells during the all experimental period. These B cells mainly existed in the lamina propria, and some cells were recognized in the interepithelial space. After 14 days p.i., small number of IgG+ and IgM+ cells were detected in the germinal center of lymph follicles in the lamina propria. From 24 to 60 hr p.i., a few number of CD3+, CD4+ and CD8+ cells were detected at the perivascular area in the lamina propria. After 3 or 4 days p.i., each positive T-cells increased rapidly in number, and reached on the peak at 5 days p.i. CD3+, CD4+ and CD8+ cells tend to distribute diffusely, perivascular area, and surrounding area of CD4+ cells, respectively. CD4+ cells were dramatically decreased from 7 days p.i., and CD3+ and CD8+ cells were decreased from 14 days p.i. No T-cells were detected in the lymph follicles in the lamina propria.
Kotani
T
T
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
Wada
S
S
Tsukamoto
Y
Y
Kuwamura
M
M
Yamate
J
J
Sakuma
S
S
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000473:pathology*; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D002645:Chickens; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary*; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D001351:Infectious bronchitis virus / Q000502:physiology; D007700:Kinetics; D011201:Poultry Diseases / Q000473:pathology*; D016176:T-Lymphocyte Subsets / Q000473:pathology*; D014132:Trachea / Q000473:pathology*; D014779:Virus Replication
D000956:Antigens, Viral; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M