Cell-cell coupling in cultures of striatal and cortical astrocytes of the monkey Cebus apella.
Astrocytes were cultured from striatum and neocortex of fetal (embryonic day 90) monkeys (Cebus apella). The cultures grew well, and the cells retained viability after freeze-storage and thawing. The cells displayed depolarized membrane potentials (-19 and -33 mV, for striatal and cortical cells, respectively) and the vast majority of cells were dye-coupled to a mean of 7 (1-18) neighbouring cells. Cell coupling was blocked by octanol (0.25 and 0.5 mM) but was independent of high K+ (10 and 50 mM) and glutamate (500 microm). Thus, cultures of fetal primate astrocytic cells are established as a model system for studies on astroglial cell-cell coupling.
Gayol
S
S
Programa Unidad de Neurobiología Aplicada (PRUNA) (CONICET), Buenos Aires, Argentina.
Pannicke
T
T
Reichenbach
A
A
Colombo
J A
JA
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002428:Cebus; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000032:analysis; D017629:Gap Junctions / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology; D007150:Immunohistochemistry; D007546:Isoquinolines / Q000032:analysis; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D019579:Neocortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000442:Octanols / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology; D014746:Vimentin / Q000378:metabolism
D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D007546:Isoquinolines; D000442:Octanols; D014746:Vimentin; D018698:Glutamic Acid; C017475:lucifer yellow; D011188:Potassium