Intermale competition in sexually mature arctic charr: effects on brain monoamines, endocrine stress responses, sex hormone levels, and behavior.
Sexually mature Arctic charr (Salvelinus alpinus) males were allowed to interact in pairs for 4 days in the absence of females. Agonistic behavior was quantified, and at the end of the experiment, plasma levels of glucose, cortisol, testosterone (T), 11-ketotestosterone (11-KT), and 17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha, 20beta-P) were determined alongside brain concentrations of serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIAA, the major 5-HT metabolite), dopamine (DA), and 3,4-dihydroxyphenylacetic acid (DOPAC, a major DA metabolite). Plasma cortisol and glucose were significantly elevated in subordinate fish, and the number of aggressive acts received showed positive correlations with plasma levels of glucose on day 1, during the development of the dominance relationship, and of cortisol on day 4, when the dominance relationship was established. In contrast, plasma concentrations of T and 11-KT were significantly higher in dominant than in subordinate males, and there was a similar tendency in plasma concentrations of 17alpha,20beta-P. Further, plasma levels of these gonadal steroids were correlated with the number of aggressive acts performed on day 4, but not with the number of aggressive acts received. The plasma cortisol concentrations did not correlate with either 5-HIAA:5-HT or DOPAC:DA ratios in any of the brain parts analyzed. Plasma glucose levels showed positive correlations with brain 5-HIAA:5-HT ratios. Negative correlations were observed between 5-HIAA:5-HT ratios in the optic tectum and between plasma levels of T, 11-KT, and 17alpha,20beta-P. Telencephalic DOPAC/DA ratios displayed a negative correlation with plasma levels of T, 11-KT, and 17alpha,20beta-P, but only in dominant males.
Elofsson
U O
UO
Department of Limnology, Uppsala University, Sweden.
Mayer
I
I
Damsgård
B
B
Winberg
S
S
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000378:Agonistic Behavior / Q000502:physiology*; D000818:Animals; D001522:Behavior, Animal; D015306:Biogenic Monoamines / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000097:blood*; D006854:Hydrocortisone / Q000097:blood*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D012701:Serotonin / Q000378:metabolism; D012741:Sexual Maturation; D012930:Social Dominance; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood; D014337:Trout / Q000502:physiology*
D015306:Biogenic Monoamines; D001786:Blood Glucose; D012739:Gonadal Steroid Hormones; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D013739:Testosterone; D006897:Hydroxyindoleacetic Acid; C003600:11-ketotestosterone; D004298:Dopamine; D006854:Hydrocortisone