Enzyme-linked immunosorbent assay for detection of chitooligosaccharides.
In order to detect chitooligosaccharides (COS), an enzyme-linked immunosorbent assay (ELISA) was developed. A chitooligosaccharide mixture (COSM) conjugated to bovine serum albumin was used to immunize rabbits to produce an anti-COS polyclonal antibody. By use of specific antibody and COSM-horseradish peroxidase conjugate, we established a competitive direct ELISA (cdELISA) the detection limit of which was about 0.1 microg/ml. In the cdELISA, the cross-reactivities of the specific antibody toward glucosamine, chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose were 0.27, 27, 75, 75, 144, and 100%, respectively, and those toward N-acetylchitobiose, N-acetylchitotriose, N-acetylchitotetraose, N-acetylchitopentaose, and N-acetylchitohexaose were 1.58, 0.005, 1.08, 0.05, and 0.40%, respectively.
Kim
S Y
SY
Department of Food Science and Technology, College of Agricultural Life Sciences, Seoul National University, Korea.
Shon
D H
DH
Lee
K H
KH
D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D002686:Chitin / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D048271:Chitosan; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009844:Oligosaccharides / Q000032:analysis* / Q000276:immunology; D011817:Rabbits
D009844:Oligosaccharides; D002686:Chitin; D048271:Chitosan