The effect of 6-methylthiohexyl isothiocyanate isolated from Wasabia japonica (wasabi) on 4-(methylnitrosamino)-1-(3-pyridyl)-1-buatnone-induced lung tumorigenesis in mice.
The present study was undertaken to estimate the effect of 6-methylthiohexyl isothiocyanate (6MHITC) isolated from Wasabia japonica (wasabi) pretreatment on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK)-induced lung tumorigenesis in mice. Pretreatment with 6MHITC for 4 consecutive days at a daily dose of 5 micromol significantly inhibited NNK-induced O(6)-methylguanine formation in lungs at 4 h after the injection. In conjugation with this inhibitory effect, 6MHITC suppressed the increase in proliferating nuclear cell antigen level as well as ornithine decarboxylase activity at a promotion stage of NNK-induced lung tumorigenesis. Finally, this treatment of 6MHITC suppressed the NNK-induced lung tumorigenesis in mice. These results suggest that 6MHITC inhibits the development of lung tumors in mice treated with NNK, due to the suppression of initiation stage.
Yano
T
T
Division of Applied Food Research, The National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, 162-8636, Tokyo, Japan.
Yajima
S
S
Virgona
N
N
Yano
Y
Y
Otani
S
S
Kumagai
H
H
Sakurai
H
H
Kishimoto
M
M
Ichikawa
T
T
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002273:Carcinogens; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000378:metabolism; D015151:Immunoblotting; D017879:Isothiocyanates / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000139:chemically induced* / Q000517:prevention & control; D051379:Mice; D009602:Nitrosamines; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D017365:Spices
D002273:Carcinogens; D017879:Isothiocyanates; D009602:Nitrosamines; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; C037152:isothiocyanic acid; D006147:Guanine; C410683:6-methylthiohexyl isothiocyanate; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; C008449:O-(6)-methylguanine; D009955:Ornithine Decarboxylase