Monoclonal antibodies suitable for type-specific identification of herpes simplex viruses by a rapid culture assay.
Eight monoclonal antibodies (MAbs) to herpes simplex viruses 1 and/or 2 (HSV-1, HSV-2) were tested for their reactivity with 190 previously typed HSV-positive clinical specimens in order to determine their suitability for use as type-specific reagents. Using a rapid culture assay we found that two MAbs (T51 and T96) identified HSV-1 in all the 94 specimens, previously found HSV-1-positive, but did not react with the 96 specimens, previously found HSV-2-positive. In contrast, one MAb (303) confirmed the presence of HSV-2 in all the specimens, previously found HSV-2-positive, but did not react with any of the 94 specimens, previously found HSV-1-positive. These three type-specific MAbs allow for a rapid type-specific identification of HSV in infected cultures. One type-common MAb (T111) reacted with all HSV-positive cultures. This MAb can be used as an excellent reagent for detection of HSV infection.
Bystrická
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic. virubyst@nic.savba.sk
Zatovicová
M
M
Petríková
M
M
Soláriková
L
L
Russ
G
G
Ziegler
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D002478:Cells, Cultured; D006558:Herpes Genitalis / Q000821:virology; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000145:classification* / Q000276:immunology*; D006801:Humans
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral