Retinoic acid delays transcription of human retinal pigment neuroepithelium marker genes in ARPE-19 cells.
The effect of retinoic acid on the differentiation of a human retinal pigment epithelium-derived cell line ARPE-19 was studied. Differentiation of ARPE-19 cells is delayed by retinoic acid. The minimum all-trans-retinoic acid concentration needed for delay of ARPE-19 differentiation is 1 microM. A delay of differentiation was also observed using 1 microM 9-cis or 13-cis-retinoic acid. Differentiation at the molecular level was studied by analyzing transcription of two RPE-marker genes, RPE65 and peropsin. In the presence of 1 microM retinoic acid the onset of transcription of both genes was delayed by 2-3 weeks. We conclude that all-trans-, 9-cis-, and 13-cis-retinoic acid delay differentiation of ARPE-19 cells into cells that phenotypically resemble cells from the human retinal pigment epithelium.
Janssen
J J
JJ
Department of Ophthalmology, University of Nijmegen, The Netherlands.
Kuhlmann
E D
ED
van Vugt
A H
AH
Winkens
H J
HJ
Janssen
B P
BP
Deutman
A F
AF
Driessen
C A
CA
D000199:Actins / Q000235:genetics; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015415:Biomarkers; D002352:Carrier Proteins; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005136:Eye Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012243:Rhodopsin; D014158:Transcription, Genetic / Q000187:drug effects*; D014212:Tretinoin / Q000494:pharmacology*; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase
D000199:Actins; D000970:Antineoplastic Agents; D015415:Biomarkers; D002352:Carrier Proteins; D005136:Eye Proteins; D011506:Proteins; D012333:RNA, Messenger; D012243:Rhodopsin; D014212:Tretinoin; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase; C504671:RRH protein, human