Isolation of a 41-kDa protein with cell adhesion activity for animal tumor cells from the mushroom Hypsizigus marmoreus by affinity chromatography with type IV collagen immobilized on agarose.
A type IV collagen-binding protein of 41 kDa was isolated from the mushroom Hypsizigus marmoreus and the protein was designated as HM41. The Western blotting analysis with anti-HM41 antibodies demonstrated that HM41 was unrelated to HM23, which had been shown to have an affinity for type IV collagen. The microsequence analysis of the membrane-blotted peptides generated by fragmentation with cyanogen bromide showed no homologous proteins reported. HM41 had cell adhesion-promoting activity for murine Lewis lung carcinoma LL2 cells and human fibrosarcoma HT1080 cells. These results indicate that HM41 is a hitherto undescribed fungus protein that can interact both with animal extracellular matrix protein type IV collagen and with animal tumor cells.
Shoji
Y
Y
School of Food and Nutritional Sciences, University of Shizuoka, Yada, Japan.
Isemura
M
M
Muto
H
H
Isemura
S
S
Aoyagi
Y
Y
D000363:Agaricales / Q000378:metabolism*; D000818:Animals; D018827:Carcinoma, Lewis Lung; D002448:Cell Adhesion / Q000187:drug effects; D002846:Chromatography, Affinity / Q000379:methods; D003094:Collagen / Q000378:metabolism*; D005354:Fibrosarcoma; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D016023:Integrins / Q000302:isolation & purification; D051379:Mice; D038981:Receptors, Collagen; D012685:Sepharose; D014407:Tumor Cells, Cultured
D005656:Fungal Proteins; D016023:Integrins; D038981:Receptors, Collagen; D003094:Collagen; D012685:Sepharose