Induction of parturition with prostaglandin f2 alpha as a possible model to study impaired reproductive performance in the dairy cow.
Parturitions were induced in five cows, 2 weeks before term using prostaglandin (PG) F(2alpha). Two i.m. injections were performed with an interval of 24 h. All cows calved within 5 days (average 2.7 days) after the first injection of PGF(2alpha). Out of five cows, four had retained fetal membranes (RFM). Each animal was sampled for bacteriological examination using uterine biopsies twice a week during 42 days postpartum (PP). Jugular vein blood samples were withdrawn for PGF(2alpha)-metabolite and progesterone analyses five times per day during the first week PP and eight times per 24 h during the 2nd and 3rd weeks PP. From the 4th week, the sampling interval was reduced back to five times per day. From the 5th week PP, the sampling was reduced to two times per day and sampling was terminated after day 46 PP. Only morning samples were used for progesterone analyses. From day 10 PP, ultrasonography (US) was performed every 3rd day until day 39 PP for detection of ovarian activity and follicular dynamics. The highest incidence of bacteriological species was found during the first 3 weeks PP. After the 5th week of collection, all animals were free from bacteria. The species of bacteria found were Arcanobacterium (Actinomyces) pyogenes, Escherichia coli, alpha-hemolytic streptococcae and Pasteurella multocida. Immediately after parturition, very high levels of the PG-metabolite were seen in all animals, with a sharp decrease to line of significance around days 9-12 PP. Small increases above the line of significance were detected up to day 27 PP in cows with RFM, and after that time the levels were considered to be at baseline. Low levels of progesterone were seen in four animals during the whole experimental time. In one animal, an increase was seen on day 43 PP, which was maintained until the end of the experimental period on day 46 PP. Based on US, follicular waves were detected in all animals during the experimental period. In three animals, three non-ovulatory follicular waves were detected and in two animals, four non-ovulatory follicular waves were detected during 39 days of ultrasound sessions. Based on progesterone levels, only one animal was considered to have ovulated around day 40 PP. Results from the present study indicate that reproductive performance of cows after PG-induced parturitions differs from those of spontaneous cases of RFM. Differences regarding the resumption of ovarian activity were also observed between previous studies of dexamethasone-induced parturitions and the present study.
Kask
K
K
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Centre for Reproductive Biology, Swedish University of Agricultural Sciences (SLU), P.O. Box 7039, SE-750 07, Uppsala, Sweden.kalle.kask@og.slu.se
Gustafsson
H
H
Gunnarsson
A
A
Kindahl
H
H
D000190:Actinomyces / Q000302:isolation & purification; D000818:Animals; D002417:Cattle / Q000382:microbiology / Q000502:physiology*; D015237:Dinoprost / Q000008:administration & dosage* / Q000097:blood; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D005865:Gestational Age; D007601:Jugular Veins; D007751:Labor, Induced / Q000662:veterinary*; D006080:Ovarian Follicle / Q000502:physiology; D010053:Ovary / Q000502:physiology; D016979:Pasteurella multocida / Q000302:isolation & purification; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012098:Reproduction; D013291:Streptococcus / Q000302:isolation & purification; D014599:Uterus / Q000382:microbiology
D011374:Progesterone; D015237:Dinoprost