Vitamin E in relation to lipid peroxidation in experimental septic shock.
Lipid peroxidation and antioxidant balance in the body is a crucial factor in the pathophysiology of various diseases. This study investigates the circulatory alpha-tocopherol levels and its relationship with 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha), a non-enzymatic and, 15-keto-13,14-dihydro-PGF2alpha (15-K-DH-PGF2alpha), a cyclooxygenase catalysed oxidation product of arachidonic acid in experimental septic shock in pigs. A steady decrease in alpha-tocopherol levels in plasma was observed in both survivor and non-survivor animals. A simultaneous increase of oxidative injury indicator, plasma 8-iso-PGF2alpha was seen in both groups but with a different fashion. 8-Iso-PGF2alpha levels increased steadily in the animals that died during the experiment. An early and rapid increase of plasma 15-K-DH-PGF2alpha, an inflammatory response indicator, was also observed in all animals. There was a significant difference in the kinetics of decrement of alpha-tocopherol levels and a concomitant increase in 15-K-DH-PGF2alpha levels among the non-survivors. Thus, a successive disappearance of circulatory vitamin E in conjunction with the surge of plasma isoprostanes and prostaglandins impairs the oxidant-antioxidant balance in favour of the former and may possibly have an effect on the survivality during experimental porcine septicaemia.
Basu
S
S
Department of Geriatrics/Clinical Nutrition Research, Faculty of Medicine, Uppsala University, Sweden. samar.basu@geriatrik.uu.se
Eriksson
M
M
D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D019446:Endotoxemia / Q000097:blood* / Q000201:enzymology / Q000503:physiopathology; D028441:F2-Isoprostanes; D005260:Female; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood; D012772:Shock, Septic / Q000097:blood* / Q000201:enzymology / Q000503:physiopathology; D013552:Swine; D014662:Vasoconstrictor Agents / Q000097:blood; D014810:Vitamin E / Q000097:blood / Q000502:physiology*
D028441:F2-Isoprostanes; D014662:Vasoconstrictor Agents; D014810:Vitamin E; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C075750:8-epi-prostaglandin F2alpha; D016718:Arachidonic Acid; D015237:Dinoprost; D011451:Prostaglandin-Endoperoxide Synthases