Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library.
We have applied bacteriophage display technology to construct and analyze the diversity of an IgG library of >1 x 108 clones from an adult sheep immunized against the hapten atrazine. We have identified eight new VH gene families (VH2-VH9) and five new Vkappa gene families (VkappaV-VkappaIX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.
Charlton
K A
KA
University of Aberdeen, Department of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, Scotland.
Moyle
S
S
Porter
A J
AJ
Harris
W J
WJ
D000818:Animals; D000916:Antibody Diversity / Q000235:genetics*; D001280:Atrazine / Q000276:immunology; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics / Q000276:immunology; D015723:Gene Library; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D006241:Haptens / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D008969:Molecular Sequence Data; D012756:Sheep / Q000235:genetics* / Q000276:immunology*
D006241:Haptens; D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D001280:Atrazine