Immunochemical analysis of a photoreceptor protein using anti-IP3 receptor antibody in the unicellular organism, Blepharisma.
The blepharismin-200 kD protein complex of the ciliated protozoan Blepharisma is a novel type of photosensor responsible for the step-up photophobic response of the cell. In immunoblotting assays, the 200 kD protein is weakly cross-reacted with anti-inositol triphosphate receptor antibody (anti-IP3 R antibody). Indirect immunofluorescence assays show that the pigment granules in which the blepharismin-200 kD protein complex is localized are labelled by anti-IP3 R antibody. When the anti-IP3 R antibody or antisense oligonucleotide for IP3 receptor is introduced into the living cells of Blepharisma, both the photosensitivity of the cells and content of blepharismin-200 kD protein are reduced. The results suggest that the photoreceptor 200 kD protein is possibly an IP3 receptor-like protein.
Matsuoka
T
T
Department of Biology, Kochi University, Japan.
Moriyama
N
N
Kida
A
A
Okuda
K
K
Suzuki
T
T
Kotsuki
H
H
D000595:Amino Acid Sequence; D000818:Animals; D015220:Calcium Channels / Q000276:immunology*; D016798:Ciliophora / Q000378:metabolism*; D003429:Cross Reactions; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting / Q000379:methods; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D008969:Molecular Sequence Data; D010569:Perylene / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D017956:Photoreceptor Cells, Invertebrate / Q000276:immunology; D010860:Pigments, Biological / Q000032:analysis* / Q000276:immunology; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology*
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; D010860:Pigments, Biological; D018160:Receptors, Cytoplasmic and Nuclear; C092293:blepharismin; D010569:Perylene; D015544:Inositol 1,4,5-Trisphosphate