Aflatoxins inhibit prolactin secretion by rat pituitary cells in culture.
Acute effects of aflatoxins (AF), and in particular hormonal actions, have not been examined as much as chronic toxicity. Thus, we studied the effects of specific AF on prolactin (PRL) secretion by rat pituitary cells in culture. AFB1 and AFQ1 (1 x 10(-4) M) reduced the stimulating effect of dimethyl sulfoxide on PRL secretion by cultured rat pituitary cells. The mechanism responsible for this action is still unknown, but it did not seem to be a non specific toxic effect, because AFB1, at the same concentration, did not significantly alter cell viability, as indicated by the Trypan blue dye-exclusion test.
Abdel-Haq
H
H
Department of Pharmacology of Natural Substances and General Physiology, University of Rome La Sapienza, Italy.
Giacomelli
S
S
Palmery
M
M
Leone
M G
MG
Saso
L
L
Silvestrini
B
B
D016604:Aflatoxin B1 / Q000633:toxicity*; D000348:Aflatoxins / Q000633:toxicity*; D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D004336:Drug Antagonism; D010902:Pituitary Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D014343:Trypan Blue / Q000378:metabolism
D000348:Aflatoxins; C033759:aflatoxin Q1; D011388:Prolactin; D016604:Aflatoxin B1; D014343:Trypan Blue; D004298:Dopamine; D004121:Dimethyl Sulfoxide