[Determination of antibody affinity using ELISA].
Some new approaches for the determination of antibody affinity were proposed. It was pointed out that the proposed methods are more simple, convenient, precise, and informative than that of Friguet et al. (1985). The approach that allows determination of two-valence antibodies affinity was also proposed. The example of two monovalent antibodies presented in the examined mixture was considered. It allows to estimate the affinity of both kinds of antibodies as well as to determine their concentration relations in the mixture.
Bobrovnik
S A
SA
A.B. Palladin Institute of Biochemistry, NAS of Ukraine, Kyiv. sab@biochem.kiev.ua
D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D004797:Enzyme-Linked Immunosorbent Assay
D000911:Antibodies, Monoclonal