Mild spherocytic hereditary elliptocytosis and altered levels of alpha- and gamma-adducins in beta-adducin-deficient mice.
The membrane skeleton, a dynamic network of proteins associated with the plasma membrane, determines the shape and mechanical properties of erythrocytes. Deficiencies or defects in membrane skeletal proteins are associated with inherited disorders of erythrocyte morphology and function. Adducin is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. In this work we show that deficiency of beta-adducin produces an 80% decrease of alpha-adducin and a fourfold up-regulation of gamma-adducin in erythrocytes. beta-Adducin or any other isoform generated by translation of abnormally spliced messenger RNAs could not be detected by our antibodies either in ghosts or in cytoplasm of -/- erythrocytes. Actin levels were diminished in mutant mice, suggesting alterations in the actin-spectrin junctional complexes due to the absence of adducin. Elliptocytes, ovalocytes, and occasionally spherocytes were found in the blood film of -/- mice. Hematological values showed an increase in reticulocyte counts and mean corpuscular hemoglobin concentration, decreased mean corpuscular volume and hematocrit, and normal erythrocyte counts that, associated to splenomegaly, indicate that the mice suffer from mild anemia with compensated hemolysis. These modifications are due to a loss of membrane surface and dehydration that result in an increase in the osmotic fragility of red blood cells. The marked alteration in osmotic fragility together with the predominant presence of elliptocytes is reminiscent of the human disorder called spherocytic hereditary elliptocytosis. Our results suggest that the amount of adducin remaining in the mutant animals (presumably alphagamma adducin) could be functional and might account for the mild phenotype. (Blood. 2000;95:3978-3985)
Muro
A F
AF
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Marro
M L
ML
Gajović
S
S
Porro
F
F
Luzzatto
L
L
Baralle
F E
FE
D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D002678:Chimera; D003433:Crosses, Genetic; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004612:Elliptocytosis, Hereditary / Q000097:blood* / Q000235:genetics*; D004912:Erythrocytes / Q000502:physiology*; D005260:Female; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006579:Heterozygote; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009996:Osmotic Fragility; D017701:Reticulocyte Count; D000099291:Adducins
D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D006454:Hemoglobins; D000099291:Adducins