[Functional status of cryopreserved rat liver mitochondria].
Mitochondria from the rat liver have been frozen down to -196 degrees C under protection of dimethylsulfoxide, after which they were subjected to periodical increase and decrease in temperature (cycling) in the ranges of -105 degrees C to -196 degrees C or -135 degrees to -196 degrees C. There have been observed some non-lethal damages of mitochondria which were revealed in the decrease in the respiratory control and in the ADP/O ratio after the cycling. The damage rate increased with the increase of the number of temperature decrease-increase cycles. The damages were also greater in the case when the upper limit of cycling temperature was higher than the glass transition temperature of the freezing medium (Tg = -126 degrees C). The non-lethal damages of mitochondria are explained by the influence of electric fields, appearing in the frozen sample during the temperature increase or decrease.
Zinchenko
A V
AV
Institute of Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkov.
Zinchenko
V D
VD
Musatova
I B
IB
Moiseev
V A
VA
D000818:Animals; D015925:Cryopreservation; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D010100:Oxygen; D004121:Dimethyl Sulfoxide