Genomic structure and chromosomal mapping of the mouse hic-5 gene that encodes a focal adhesion protein.
The hic-5 gene encodes a focal adhesion protein that has striking similarity to paxillin. Genomic clones of the mouse hic-5 gene were isolated, and included 10 exons that covered the whole mouse mRNA sequence. Comparison of the sequence with those in the expressed sequence tag database suggested that the hic-5 gene contained an extra exon (named exon 1') located about 1kb upstream of exon 1, and mouse cells seemed to express two alternatively spliced forms of mRNA. All the exon-intron boundaries followed the GT/AG rule. Physical mapping and fluorescent in situ hybridization analysis indicated that the hic-5 gene is located on mouse chromosome 7, 60. 0cM from the centromere.
Mashimo
J
J
Department of Microbiology, Showa University School of Pharmaceutical Sciences, Tokyo, Japan.
Shibanuma
M
M
Satoh
H
H
Chida
K
K
Nose
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D005796:Genes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D060588:LIM Domain Proteins; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D060588:LIM Domain Proteins; C090090:Tgfb1i1 protein, mouse; D004247:DNA