title: Centration of donor trephination in human corneal transplantation.
abstract: OBJECTIVE
  To develop and evaluate a new method to quantify centration of the trephined donor cornea relative to the limbus.
  
  
  METHODS
  After human donor corneas were trephined for penetrating keratoplasty, the remaining corneoscleral discs were stained and subjected to image analysis. The centration of the excised donor cornea relative to the limbus was calculated by measuring their centroids from the "captured" images.
  
  
  RESULTS
  Fifty-two corneoscleral discs were analyzed. The average deviation from the centre was 0.32 mm (SD, 0.18 mm). Neither surgeon nor the type of trephine significantly influenced the mean centroid deviation.
  
  
  CONCLUSIONS
  We have developed and evaluated a method to quantify centration of human donor cornea. In a small series, decentration did not correlate significantly with either the surgeon or the trephine.
authors.affiliation: School of Biological Sciences, Manchester University, Royal Eye Hospital, UK.
authors.forename: A
authors.initials: A
authors.lastname: Brahma
authors.forename: A
authors.initials: A
authors.lastname: Ridgway
authors.forename: A
authors.initials: A
authors.lastname: Tullo
authors.forename: M
authors.initials: M
authors.lastname: Boulton
authors.forename: G
authors.initials: G
authors.lastname: Ireland
authors.forename: S
authors.initials: S
authors.lastname: Bagley
authors.forename: L
authors.initials: L
authors.lastname: Carrington
mesh_terms: D003315:Cornea / Q000033:anatomy & histology*; D005123:Eye / Q000033:anatomy & histology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D015948:Keratoplasty, Penetrating / Q000379:methods*; D011680:Pupil; D014019:Tissue Donors