title: HIV-1 RNA levels in an African population-based cohort and their relation to CD4 lymphocyte counts and World Health Organization clinical staging.
abstract: Apart from a small number of reports from people who are based in hospitals, data on viral load in HIV-infected people in sub-Saharan Africa, where most infections occur, are lacking. We report serum HIV-1 RNA levels in a population-based cohort in rural Uganda using the nucleic acid sequence-based amplification procedure (NASBA) test kit and describe their relation to CD4 counts and World Health Organization (WHO) clinical staging. The median (interquartile range [IQR]) viral loads were 87,000 copies/ml (37,500-295,000 copies/ml) in 40 prevalent cases infected for >6 years, and 31,000 copies/ml (7800-174,000 copies/ml) in 65 incident cases with seroconversion dates within the previous 6 years. Although we found a correlation between viral load and absolute CD4 count (p < .0001), there was no evidence for an association with CD4 decline (p = .1). Overall, there was a significant trend of increasing viral load with worsening clinical stage from a median viral load of 15,000 for those in WHO stage 1 (asymptomatic) to 150,600 copies/ml for those in stage 4 (AIDS; p < .001). However, the association was seen only in incident cases. Thus, we found that the NASBA test on serum was a useful indicator of disease stage especially in persons known to be infected for <6 years. Such baseline data are important for vaccine research, and if antiretroviral drugs become available to more than a few people in Africa, it will be important that accurate viral load estimations are available at least in a proportion of people to monitor the effectiveness of treatment, and measure the compliance and emerging resistance to these drugs.
authors.affiliation: Medical Research Council Programme on AIDS/Uganda Virus Research Institute, Entebbe. mrc@starcom.co.ug
authors.forename: D
authors.initials: D
authors.lastname: Morgan
authors.forename: A
authors.initials: A
authors.lastname: Rutebemberwa
authors.forename: S
authors.initials: S
authors.lastname: Malamba
authors.forename: A
authors.initials: A
authors.lastname: Ross
authors.forename: J
authors.initials: J
authors.lastname: Whitworth
authors.forename: P
authors.initials: P
authors.lastname: Kaleebu
authors.forename: F
authors.initials: F
authors.lastname: Gotch
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D015331:Cohort Studies; D005260:Female; D015658:HIV Infections / Q000276:immunology* / Q000401:mortality / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D012367:RNA, Viral / Q000032:analysis*; D012423:Rural Health; D014454:Uganda; D019562:Viral Load; D014944:World Health Organization
chemical_list: D012367:RNA, Viral