Identification of actin from hepatoma Morris 5123 cells.
The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118; Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit a skeletal muscle actin (Mr 43,000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti beta actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.
Nowak
D
D
Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, Poland.
Kochman
A
A
Malicka-Błaszkiewicz
M
M
D000199:Actins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D003599:Cytoskeleton / Q000737:chemistry; D003600:Cytosol / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008114:Liver Neoplasms, Experimental / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D020033:Protein Isoforms / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011915:Rats, Inbred BUF
D000199:Actins; D000911:Antibodies, Monoclonal; D020033:Protein Isoforms