Increase of hybrids in yeast electrofusion by mitochondrial mutations.
Yeast mitochondria were found to affect the zeta-potential of protoplasts, resulting in electrofusion of membrane behavior. For modeling purposes, two fusion systems were investigated: (1) parent yeasts of Saccharomyces cerevisiae G706 (rho(+)) x O708-11-16A (rho(+)), with zeta-potentials of -10 to -27 mV and -28 to -45 mV, depending on MgCl(2) concentration in the medium, respectively; and (2) parent yeasts of S. cerevisiae G706-1 (rho(-)) x O708-11-16A (rho(+)), with zeta-potentials of -30 to -60 mV, depending on MgCl(2). Yields of the hybrids in system (2) were over 100-fold higher than those in system (1). Thus, regulation of yeast electrofusion was found to be possible by mitochondrial mutations.
Urano
N
N
Laboratory of Marine Biochemistry, Tokyo University of Fisheries, 5-7 Konan-4, Minato, Tokyo 108-8477, Japan. urano@tokyo-u-fish.ac.jp
Ueno
R
R
Kimura
S
S
D002459:Cell Fusion; D003470:Culture Media; D008928:Mitochondria / Q000235:genetics*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
D003470:Culture Media