title: Identification of a high frequency of chromosomal rearrangements in the centromeric regions of prostate cancer cell lines by sequential giemsa banding and spectral karyotyping.
abstract: BACKGROUND
  Currently, prostate cancer (CaP) cytogenetics is not well defined, largely because of technical difficulties in obtaining primary tumor metaphases.
  
  
  RESULTS
  We examined three CaP cell lines (LNCaP, DU145, PC-3) using sequential Giemsa banding and spectral karyotyping (SKY) to search for a common structural aberration or translocation breakpoint. No consistent rearrangement common to all three cell lines was detected. A clustering of centromeric translocation breakpoints was detected in chromosomes 4, 5, 6, 8, 11, 12, 14, and 15 in DU145 and PC-3. Both these lines were found to have karyotypes with a greater level of complexity than LNCaP.
  
  
  CONCLUSIONS
  The large number of structural aberrations present in DU145 and PC-3 implicate an underlying chromosomal instability and subsequent accumulation of cytogenetic alterations that confer a selective growth advantage. The high frequency of centromeric rearrangements in these lines indicates a potential role for mitotic irregularities associated with the centromere in CaP tumorigenesis.
authors.affiliation: Department of Laboratory Medicine and Pathobiology, Ontario Cancer Institute, University of Toronto, Toronto, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Beheshti
authors.forename: J
authors.initials: J
authors.lastname: Karaskova
authors.forename: P C
authors.initials: PC
authors.lastname: Park
authors.forename: J A
authors.initials: JA
authors.lastname: Squire
authors.forename: B G
authors.initials: BG
authors.lastname: Beatty
mesh_terms: D001399:Azure Stains / Q000378:metabolism; D002503:Centromere; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D025063:Chromosome Disorders; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D001399:Azure Stains