Comparison of trabecular bone architecture in young and old bones.
In this study, different parameters currently applied for the description of trabecular bone architecture in young and old subjects are compared. Moreover, new parameters are proposed and assessed. For the investigations, the two-dimensional images of vertebral body sections are acquired with the use of a low-magnification digital camera. The parameters were calculated both for the skeleton and for the bone/marrow interface distinguished in the images of the trabecular network. The following methods of the descriptions of the trabecular bone architecture were considered: histomorphometric analysis (BV/TV, asymmetry, mean trabeculae length), fractal geometry technique, Euler characteristic, star volume of the marrow cavity, the mean distance between two points of the trabecular network, and the probability of disconnection (is straightforwardly connected with the number of separated parts of the network). Moreover, bone mineral density (BMD) was determined for comparative purposes. The quantities directly extracted form the images are also combined to produce new indexes, which better differentiate young and old bones. It was found that the BMD, the BV/TV, the star volume, the Euler number, and the probability of disconnection might be used as indicators of the age-related changes of trabecular bone. The parameters could be measured with the precision comparable to that of the BMD. The only exception is the probability of disconnection. Highly significant correlations were demonstrated between bone density (BMD, BV/TV) and trabecular architecture (SV/GV, probability of disconnection).
Tabor
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Z
Institute of Physics, Jagiellonian University, Cracow, Poland.
Rokita
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E
D000328:Adult; D000368:Aged; D000375:Aging / Q000378:metabolism / Q000473:pathology*; D055592:Biophysical Phenomena; D001703:Biophysics; D015519:Bone Density; D001842:Bone and Bones / Q000033:anatomy & histology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008953:Models, Anatomic