Synthesis of a chemiluminescent acridinium hydroxylamine (AHA) for the direct detection of abasic sites in DNA.
[structure: see text] The synthesis of a chemiluminescent acridinium hydroxylamine (AHA) for the direct detection of abasic sites in damaged nucleic acids is described. The reagent reacts readily with abasic sites of damaged calf thymus DNA generated in a time-dependent manner under acid/heat depurination conditions. Preliminary results indicate the sensitivity of the direct chemiluminescent detection format is approximately 0.1 abasic sites detected per 10(6) nucleotides using as little as 200 ng of DNA.
Adamczyk
M
M
Department of Chemistry, Diagnostics Division, Abbott Laboratories, 100 Abbott Park Road, Department 09NM, Bldg. AP20, Abbott Park, Illinois 60064-6016, USA. maciej.adamczyk@abbott.com
Mattingly
P G
PG
Moore
J A
JA
Pan
Y
Y
D000166:Acridines / Q000138:chemical synthesis*; D000478:Alkylation; D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D006898:Hydroxylamines / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D008163:Luminescent Measurements
D000166:Acridines; D006898:Hydroxylamines; D007202:Indicators and Reagents; D004247:DNA