Surface expression of HLA-C antigen by human extravillous trophoblast.
In this paper definitive evidence that the classical class I product, HLA-C, is expressed on the surface of normal trophoblast cells is provided. HLA-C transcripts were sequenced from cDNA isolated from first trimester trophoblast cells obtained by flow cytometric sorting. Both paternal and maternal alleles were transcribed. HLA-C proteins were demonstrated by biochemical analysis and found on the cell surface in association with beta(2)-microglobulin. Upregulation of cell surface HLA-C but not HLA-G expression after interferon (IFN)-gamma treatment was demonstrated by flow cytometric analysis. Immunohistology has confirmed HLA-C is expressed by all extravillous subpopulations in vivo. The question of whether trophoblast HLA-C molecules interact with decidual NK cells expressing killer Ig-like receptors (KIR) has also been addressed. Our results demonstrate that extravillous trophoblast expresses at least two HLA class I molecules, HLA-G and HLA-C on the cell surface.
King
A
A
Research Group in Human Reproductive Immunobiology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK. akk27@cam.ac.uk
Burrows
T D
TD
Hiby
S E
SE
Bowen
J M
JM
Joseph
S
S
Verma
S
S
Lim
P B
PB
Gardner
L
L
Le Bouteiller
P
P
Ziegler
A
A
Uchanska-Ziegler
B
B
Loke
Y W
YW
D000328:Adult; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics; D002822:Choriocarcinoma / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006680:HLA Antigens / Q000096:biosynthesis; D015236:HLA-C Antigens / Q000096:biosynthesis* / Q000235:genetics; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism; D054340:Receptors, KIR; D054342:Receptors, KIR2DL1; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D001613:beta 2-Microglobulin / Q000378:metabolism
D000954:Antigens, Surface; D006680:HLA Antigens; D015236:HLA-C Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; C093100:KIR2DL1 protein, human; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054342:Receptors, KIR2DL1; D011994:Recombinant Proteins; D001613:beta 2-Microglobulin; D007371:Interferon-gamma