Experimental intravaginal infection of goats with caprine herpesvirus 1.
Three adult goats, seronegative to caprine herpesvirus 1 (CpHV.1), were intravaginally inoculated with BA.1 strain of CpHV.1. The animals were kept under observation for 1 month and daily both clinical examinations and white blood cell count were performed. Ocular, nasal, rectal and vaginal swabs and heparinized blood samples were collected every day to attempt virus isolation on cell cultures and detect viral DNA by polymerase chain reaction (PCR) assay. The virus was isolated and detected by PCR only from the vaginal swabs for 5-7 days post-infection. The animals showed transient fever and leukopenia and typical necrotic lesions on the vulva and vagina.
Tempesta
M
M
Department of Health and Animal Welfare, Faculty of Veterinary Medicine, Bari, Italy.
Pratelli
A
A
Normanno
G
G
Camero
M
M
Buonavoglia
D
D
Greco
G
G
Buonavoglia
C
C
D000818:Animals; D004279:DNA, Viral / Q000302:isolation & purification*; D005260:Female; D015511:Goat Diseases / Q000821:virology*; D006041:Goats; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D007958:Leukocyte Count / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014621:Vagina / Q000473:pathology / Q000821:virology; D014623:Vaginal Diseases / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral