title: Onchocerca volvulus: comparative analysis of antibody responses to recombinant antigens in two animal models of onchocerciasis.
abstract: Experimental infections of chimpanzees with Onchocerca volvulus and cattle with Onchocerca ochengi provide model systems for research in human onchocerciasis. These infections share many similarities from the standpoint of parasite biology, but little is known about the comparability of immune responses in the two systems. To make a direct comparison between the models in terms of immune responsiveness to defined parasite products, three recombinant antigens of O. volvulus (Ov7, Ov103, and B20) were used to analyze the kinetics of antibody production following experimental infection. Each of the antigens was derived from adult cDNA libraries following immunoscreening with sera from chimpanzees (Ov7, Ov103) or cattle (B20). All chimpanzees (n = 12) and cattle (n = 8) displayed responses to Ov7 and Ov103, and all cattle, but only 33% of chimpanzees, showed responses to B20. The dynamics of the response to individual antigens showed further similarities between the chimpanzees and the cattle, with responses to Ov7 and Ov103 peaking after, and B20 before, the onset of patent infections. We conclude that there is good preliminary evidence of concordance in the kinetics of serological responses in the two models. However, individual antigens many be more or less immunogenic in the two systems, making it inadvisable to extrapolate between models concerning the relative immunodominance of specific parasite products.
authors.affiliation: Liverpool School of Tropical Medicine, Liverpool, L3 5QA, United Kingdom.
authors.forename: S P
authors.initials: SP
authors.lastname: Graham
authors.forename: S
authors.initials: S
authors.lastname: Lustigman
authors.forename: A J
authors.initials: AJ
authors.lastname: Trees
authors.forename: A E
authors.initials: AE
authors.lastname: Bianco
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D017181:Onchocerca volvulus / Q000235:genetics / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology*; D002679:Pan troglodytes; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins