title: T cell deletion and unresponsiveness induced by intrathymic injection of staphylococcal enterotoxin B.
abstract: Intrathymic injection of alloantigens appears to be the most efficient route to induce alterations of T cell reactivity. In the present study, we explored the modifications of Vbeta8.1, 8.2 T cell population and T cell reactivity in the thymus and in the spleen induced by intrathymic injection of staphylococcal enterotoxin B to adult mice. Vbeta8 antigen expression was investigated by flow cytometry analysis. T Cell reactivity was studied in vitro by the proliferative response to SEB. SEB induced a significant reduction in the percentage of mature Vbeta8+ T cells in the thymus (days 7-14), and in the spleen (days 7-28). Interestingly, this depletion occurs in the CD4- CD8+ cells in the thymus whereas in the CD4+ CD8- cells in the spleen. In parallel, the proliferative response to SEB but not to SEA was significantly decreased in the thymus on days 7 and 14, and in the spleen from day 7 to day 28. Moreover, this unresponsiveness was more pronounced in the spleen than in the thymus. Anergy was SEB-specific and fully reversed by exogenous IL-2. SEB injected intrathymically induced significantly more pronounced and more durable T cell alterations than intraperitoneal and subcutaneous injections. This may be related to the observation that after i.t. injection, SEB was detected both at a higher amount and for a longer period in the central and peripheral compartments. Our results clearly demonstrate that the intrathymic route is definitely the most efficient to induce not only thymic but also peripheral pivotal immune alterations in our model.
authors.affiliation: Laboratoire de Physiologie Thymique CNRS ESA 8078, Université Paris-Sud, Centre Chirurgical Marie-Lannelongue, Le Plessis-Robinson, France. clplanch@wanadoo.fr
authors.forename: P
authors.initials: P
authors.lastname: Goettelfinger
authors.forename: R
authors.initials: R
authors.lastname: Roussin
authors.forename: F
authors.initials: F
authors.lastname: Lecerf
authors.forename: S
authors.initials: S
authors.lastname: Berrih-Aknin
authors.forename: M
authors.initials: M
authors.lastname: Fattal-German
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D004768:Enterotoxins / Q000276:immunology*; D007267:Injections; D007376:Interleukin-2 / Q000276:immunology; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*; D013997:Time Factors
chemical_list: D004768:Enterotoxins; D007376:Interleukin-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; C075784:T cell receptor Vbeta8; C031818:enterotoxin B, staphylococcal