Determination of potato glycoalkaloids using a liposome immunomigration, liquid-phase competition immunoassay.
Polyclonal anti-solanine antibodies were raised and used in the assembly of a liposome immunomigration, liquid-phase competition strip immunoassay. In this format, a similar cross-reactivity was observed between the glycoalkaloids alpha-solanine and alpha-chaconine. The strip assay was implemented to quantitate total glycoalkaloids (TGA) from potatoes. Recoveries in spiked potato samples using the strip assay and water:acetic acid:sodium bisulfite extracting solvent were in the range of 84-111%. The values of the TGA quantitations by the strip assay as compared with those obtained by HPLC, in nonspiked tubers coming from cloned potato samples donated by potato breeders, were equivalent and highly correlated (r(2) = 0.91). The strip assay proved advantageous over HPLC for extra-laboratory measurements such as the rapid identification of samples that should be rejected due to an elevated TGA content.
Glorio-Paulet
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Department of Food Science and Technology, Cornell University, Geneva, New York 14456-0462, USA. pgp@lamolina.edu.pe
Durst
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D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D007118:Immunoassay / Q000379:methods*; D008081:Liposomes; D011198:Solanum tuberosum / Q000737:chemistry*
D000470:Alkaloids; D008081:Liposomes