Multifrequency method for dielectric monitoring of cold-preserved organs.
To answer a growing need for non-invasive monitoring of biological organs, we have developed an automated system capable of repeated dielectric measurements over the frequency range 10 kHz-100 MHz. Further, we propose a novel method of data analysis that may convert the acquired, individual dispersion curves into a diagram of the time course of specific phenomenological parameters, such as the characteristic frequency. By using this new procedure, unattended, long-term monitoring of temporal changes in the dielectric behaviour of excised liver lobes stored at 4 degrees C was successfully realized. The 'multifrequency' method presented here was definitely superior to the conventional 'fixed-frequency' method in providing reliable results.
Raicu
V
V
Department of Physiology, Kochi Medical School, Japan. vraicu@pop.med.kochi-ms.ac.jp
Saibara
T
T
Irimajiri
A
A
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D003080:Cold Temperature; D004594:Electrophysiology; D066298:In Vitro Techniques; D008099:Liver / Q000502:physiology; D008297:Male; D008954:Models, Biological; D008991:Monitoring, Physiologic / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors