title: Effect of somatostatin on electrogenic ion transport in the duodenum and colon of the mouse, Mus domesticus.
abstract: In this study, we have used the mouse intestine and the Ussing short circuit technique to compare the effects and mechanism of action of somatostatin (SST, 0.1 microM) on cAMP- and Ca(2+)-mediated ion secretion in the duodenum and colon of the Swiss-Webster mouse. The cAMP-dependent secretagogues, prostaglandin E(2) (1 microM) and dibutyryl-cAMP (150 microM) increased short circuit current (I(sc)) in both regions, but only the colonic response was inhibited by SST. This inhibition was independent of enteric nerves, suggesting a direct action on the epithelial cells. The Ca(2+)-dependent secretagogue carbachol (10 microM) stimulated a transient increase in I(sc) in both intestinal segments. In the duodenum, SST partially inhibited this increase in I(sc) and both the responses to carbachol and SST were independent of enteric nerves. In the colon, while SST inhibited the carbachol induced increase in I(sc), pre-treatment with tetrodotoxin (750 nM) profoundly inhibited the carbachol induced increase in I(sc), thus markedly reducing the inhibitory effect of SST. This indicates an involvement of the enteric nervous system in the response to carbachol and the action of SST in the colon. These data indicate marked regional differences within the mouse intestine of the effects of SST on ion secretion and demonstrate different mechanisms of action of SST in the duodenum and colon.
authors.affiliation: Department of Physiology, School of Medical Sciences, University of Otago, P.O. Box 913, Dunedin, New Zealand.
authors.forename: A
authors.initials: A
authors.lastname: Samson
authors.forename: K L
authors.initials: KL
authors.lastname: Hamilton
authors.forename: A G
authors.initials: AG
authors.lastname: Butt
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D003106:Colon / Q000378:metabolism* / Q000502:physiology; D000242:Cyclic AMP / Q000502:physiology; D015232:Dinoprostone / Q000494:pharmacology; D004386:Duodenum / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D017615:Enteric Nervous System / Q000187:drug effects; D017136:Ion Transport; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D013004:Somatostatin / Q000494:pharmacology*
chemical_list: D013004:Somatostatin; D002217:Carbachol; D000242:Cyclic AMP; D015232:Dinoprostone; D002118:Calcium