title: Intergeneric poliovirus recombinants for the treatment of malignant glioma.
abstract: Poliovirus neuropathogenicity depends on sequences within the 5' nontranslated region of the virus. Exchange of the poliovirus internal ribosomal entry site with its counterpart from human rhinovirus type 2 resulted in attenuation of neurovirulence in primates. Despite deficient virus propagation in cells of neuronal origin, nonpathogenic polio recombinants retain excellent growth characteristics in cell lines derived from glial neoplasms. Susceptibility of malignant glioma cells to poliovirus may be mediated by expression of a poliovirus receptor, CD155, in glial neoplasms. Intergeneric polio recombinants with heterologous internal ribosomal entry site elements unfolded strong oncolytic potential against experimentally induced gliomas in athymic mice. Our observations suggest that highly attenuated poliovirus recombinants may have applicability as biotherapeutic antineoplastic agents.
authors.affiliation: Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, NY 11794, USA.
authors.forename: M
authors.initials: M
authors.lastname: Gromeier
authors.forename: S
authors.initials: S
authors.lastname: Lachmann
authors.forename: M R
authors.initials: MR
authors.lastname: Rosenfeld
authors.forename: P H
authors.initials: PH
authors.lastname: Gutin
authors.forename: E
authors.initials: E
authors.lastname: Wimmer
mesh_terms: D000818:Animals; D005910:Glioma / Q000473:pathology / Q000628:therapy* / Q000821:virology; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D017955:Poliovirus / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000502:physiology; D012229:Rhinovirus / Q000502:physiology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014779:Virus Replication
chemical_list: D008565:Membrane Proteins; D011991:Receptors, Virus; C066100:poliovirus receptor