The role of ferrioxamine E in pre-enrichment medium for determining Salmonella in environmental samples according to ISO method.
The effect of a siderophoric compound, ferrioxamine E, in the pre-enrichment broth on determining of Salmonella infantis in environmental samples was tested with combination of various pre-enrichment times and enrichment temperatures of 37 and 43 degrees C. Ferrioxamine E slightly improved the determination efficiency of this bacterium but the pre-enrichment time could not be reduced below 17 hours. The enrichment temperature of 43 degrees C was better than of 37 degrees C. The mixing ratios of 1:100 or 1:1000 for samples and pre-enrichment broth were more successful than the ratio of 1:10 as recommended by ISO.
Heinonen-Tanski
H
H
Department of Environmental Sciences, University of Kuopio, Finland. heinotan@uku.fi
Philipp
W
W
Böhm
R
R
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle / Q000382:microbiology; D003470:Culture Media; D004783:Environmental Microbiology; D005290:Ferric Compounds / Q000378:metabolism*; D008372:Manure / Q000382:microbiology; D010456:Peptides, Cyclic / Q000378:metabolism*; D012475:Salmonella / Q000145:classification* / Q000254:growth & development* / Q000302:isolation & purification; D012722:Sewage; D014866:Waste Products
D003470:Culture Media; D005290:Ferric Compounds; D008372:Manure; D010456:Peptides, Cyclic; D012722:Sewage; D014866:Waste Products; C017508:ferrioxamine E