The augmentative effect of repeated heat shock preconditioning on the production of heat shock protein 72 and on ischemic tolerance in rat liver tissue.
OBJECTIVE
Heat shock pretreatment induces heat shock protein (HSP)72 strongly in rat livers and provides the tolerance against subsequent ischemia-reperfusion injury. In this study, the effects of repeated heat shock pretreatment on the production of HSP72 in rat livers and on subsequent ischemic tolerance were investigated.


METHODS
Rats pretreated with repeated heat shock were compared with those that received a single heat shock pretreatment. The production of HSP72 was analysed using Western-blotting and densitometer. At 48 h after heat shock pretreatment, all rats were subjected to warm liver ischemia for 30 or 45 min and then reperfused. Survival rate of the animals and liver functions during reperfusion were analysed.


RESULTS
The production of HSP72 increased in the repeated heat shock group more than in the single heat shock group. Although there were no significant differences in animal survival or in liver functions after a 30-min ischemia between the single heat shock group and the repeated heat shock group, animal survival and liver functions after a 45-min ischemia were significantly better in the repeated heat shock group.


CONCLUSIONS
In rats, repetition of heat shock pretreatment augmented the production of HSP72 in liver tissue and protected the liver from ischemia-reperfusion injury.
Kimoto
S
S
Department of Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Japan.
Yamamoto
Y
Y
Yamagami
K
K
Ishikawa
Y
Y
Kume
M
M
Yamamoto
H
H
Ozaki
N
N
Yamaoka
Y
Y
D000818:Animals; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature / Q000627:therapeutic use*; D007150:Immunohistochemistry; D007511:Ischemia / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000293:injuries* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins