title: Immunohistochemical study of dental pulp applied with 4-META/MMA-TBB adhesive resin after pulpotomy.
abstract: The purpose of this study was to investigate nerve regeneration and proliferative activity in amputated pulp tissue after the application of 4-META/MMA-TBB adhesive resin (4-META resin). Calcium hydroxide was used as a control material. At 3 days, fibroblast-like cells were positive for proliferating cell nuclear antigen (PCNA) in both 4-META resin- and calcium hydroxide-treated groups and were located mainly within 0.5 mm from the cut surface. Only a few fragmented neurofilament protein (NFP)-positive nerve fibers were observed in this area. At 7 and 14 days, the number of PCNA-positive cells had gradually decreased and regenerated NFP-positive nerve fibers were observed close to the cut surface of the pulp in both groups. At 21 days in the experimental group, several PCNA-positive cells were still found in the area 0.5 mm from the cut surface, and NFP-positive nerve fibers were detected about 0.15-;0.2 mm from the cut surface. In contrast, a dentin bridge was produced under the necrotic layer at 21 days in the control group. PCNA-positive cells were not found underneath the dentin bridge, but NFP-positive nerve fibers had regenerated close to it. These results suggest that although cell differentiation and nerve regeneration are delayed, wound healing occurred even after the application of 4-META resin to exposed pulp surface the same as calcium hydroxide application.
authors.affiliation: Oral Health Science Center, Department of Pathology, Tokyo Dental College, 1-2-2, Masago, Mihama, Chiba, 261-8502, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Shimono
mesh_terms: D000818:Animals; D001896:Boron Compounds; D002126:Calcium Hydroxide; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D003782:Dental Pulp / Q000166:cytology* / Q000473:pathology; D004285:Dogs; D004727:Endothelium / Q000166:cytology; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D007150:Immunohistochemistry; D008648:Mesoderm / Q000166:cytology; D008689:Methacrylates; D008768:Methylmethacrylates; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011672:Pulpotomy; D019279:Resin Cements
chemical_list: D001896:Boron Compounds; D008689:Methacrylates; D008768:Methylmethacrylates; D018809:Proliferating Cell Nuclear Antigen; D019279:Resin Cements; C049495:Super-bond; D002126:Calcium Hydroxide