title: Differential expression of alternatively spliced neural cell adhesion molecule L1 isoforms during oligodendrocyte maturation.
abstract: The expression of neural cell adhesion molecules and myelin-specific molecules is precisely regulated according to cell type and developmental age. We investigated whether different isoforms of these molecules change during development of oligodendrocytes. Immature oligodendrocytes cultured from embryonic day 18 rat cerebrum were distinguished into early stage and late stage by morphological and immunocytochemical criteria. mRNA levels of the neural cell adhesion molecule L1 in late-stage immature oligodendrocytes were approximately fivefold higher than in early-stage cells, but early-stage immature oligodendrocytes predominantly expressed an L1 spliced isoform lacking two region (exon 2 and 27). Late-stage cells expressed full-length L1 identical to the neuronal form. mRNA for the neural cell adhesion molecules NCAM and MAG did not show any difference in expression pattern. These results suggest that alternatively spliced isoforms of L1 might be regulated by temporal and spatial factors during oligodendrocyte development.
authors.affiliation: Department of Molecular Biodynamics, The Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Bunkyo-ku, Tokyo, Japan. itoh@rinshoken.or.jp
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Sakurai
authors.forename: H
authors.initials: H
authors.lastname: Asou
authors.forename: M
authors.initials: M
authors.lastname: Umeda
mesh_terms: D000367:Age Factors; D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005091:Exons / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins / Q000235:genetics*; D004676:Myelin Basic Protein / Q000032:analysis / Q000235:genetics; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D018992:Myelin-Associated Glycoprotein / Q000235:genetics; D019006:Neural Cell Adhesion Molecules / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy; D020033:Protein Isoforms / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D004676:Myelin Basic Protein; D018992:Myelin-Associated Glycoprotein; D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms; D012333:RNA, Messenger