title: Yersinia enterocolitica-mediated translocation of defined fusion proteins to the cytosol of mammalian cells results in peptide-specific MHC class I-restricted antigen presentation.
abstract: Yersinia enterocolitica delivers a set of effector proteins [Yersinia outer proteins (Yop)] into the cytosol of target cells to modulate host cell signal transduction pathways required for the extracellular survival of the bacterium. Secretion and subsequent translocation of Yop across the eukaryotic cell membrane are achieved via a type III secretion system. About 50 - 100 amino acids of the N terminus of Yop are required for chaperone-directed secretion and translocation. In this study, it is demonstrated by immunoblot analysis of Yersinia-infected cultured epithelial cells that one ot these proteins, YopE, can serve as a molecular carrier to deliver protein fragments of the heterologous p60 antigen of Listeria monocytogenes into the cytosol of target cells. T cell activation assays revealed that the observed type III-mediated antigen translocation led to a p60 peptide-specific MHC class I-restricted antigen presentation. Efficient translocation and antigen presentation were strictly dependent on the co-localized expression of hybrid YopE-p60 proteins and the YopE-specific chaperone SycE. These results suggest that the Yersinia type III secretion system may serve as an attractive tool for antigen delivery in Yersinia-based live vaccines to induce cellular immune responses.
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universität München, München, Germany. ruessman@m3401.mpk.med.uni-muenchen.de
authors.forename: H
authors.initials: H
authors.lastname: Rüssmann
authors.forename: A
authors.initials: A
authors.lastname: Weissmüller
authors.forename: G
authors.initials: G
authors.lastname: Geginat
authors.forename: E I
authors.initials: EI
authors.lastname: Igwe
authors.forename: A
authors.initials: A
authors.lastname: Roggenkamp
authors.forename: A
authors.initials: A
authors.lastname: Bubert
authors.forename: W
authors.initials: W
authors.lastname: Goebel
authors.forename: H
authors.initials: H
authors.lastname: Hof
authors.forename: J
authors.initials: J
authors.lastname: Heesemann
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D018988:Bacterial Translocation; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D015009:Yersinia Infections / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D015395:Histocompatibility Antigens Class I; D010455:Peptides; C063239:yopE protein, Yersinia