Fast chiral separations using sulfated beta-cyclodextrin and short-end injection in capillary electrophoresis.
The general applicability of sulfated beta-cyclodextrin as chiral selector and short-end injection in capillary electrophoresis (CE) as a powerful screening tool for fast and efficient chiral separation of Ormeloxifene enantiomers and racemic Ormeloxifene analogues is demonstrated. Using the short-end injection procedure, all of the 16 racemic compounds studied were successfully separated with high efficiencies and with analysis times of less than 1.2 min. Furthermore, long-end injections of eight analogues named C1-C8 afforded separations with extremely high efficiencies. A statistical evaluation of the resolution values obtained in short-end and long-end injections of compounds C1-C8 showed that the sensitivity of the CE method towards structural changes in the studied molecules is intact when the chiral analysis is performed with short-end injection compared to conventional long-end injection.
Bergholdt
A B
AB
Analytical Development, Pharmaceuticals Development, Novo Nordisk A/S, Maaloev, Denmark.
Jørgensen
K W
KW
Wendel
L
L
Lehmann
S V
SV
D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D013464:Sulfuric Acids / Q000737:chemistry; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D013464:Sulfuric Acids; D047392:beta-Cyclodextrins; C031215:betadex