Participation of neuropeptides in antigen-induced contraction of guinea pig bronchi via NK(2) but not NK(1) receptor stimulation.
We evaluated the contribution of neuropeptides to antigen-induced contractions of isolated bronchi and tracheae of passively sensitized guinea pigs using CP-96345 and SR 48968, specific antagonists of NK(1) and NK(2) receptors, respectively, in combination with treatment by an antihistaminic and a cysteinyl leukotriene antagonist. SR-48968 but not CP-96345, significantly inhibited the late phase of the bronchial contraction. Phosphoramidon, a neutral endopeptidase inhibitor, tended to potentiate bronchial contraction. Posttreatment with SR-48968 decreased the enhanced contraction induced by the inhibitor as well as the nonenhanced contraction to similar levels of tension. On the other hand, antigen-induced tracheal contraction was not altered by either neuropeptide antagonist. These results suggest that neuropeptides mediate the antigen-induced contractile response of the guinea pig bronchus partly through NK(2) receptor stimulation.
Tanpo
T
T
Department of Pharmacology, Kyoto Pharmaceutical University, Kyoto, Japan.
Nabe
T
T
Yasui
K
K
Kamiki
T
T
Kohno
S
S
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D044482:Benzeneacetamides; D001713:Biphenyl Compounds / Q000494:pharmacology*; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D016084:Bronchoconstriction / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D020024:Leukotriene Antagonists / Q000494:pharmacology; D008297:Male; D064729:Neurokinin-1 Receptor Antagonists; D009479:Neuropeptides / Q000187:drug effects* / Q000378:metabolism; D010400:Penicillin G / Q000031:analogs & derivatives / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology*; D011738:Pyrilamine / Q000494:pharmacology; D018041:Receptors, Neurokinin-2 / Q000037:antagonists & inhibitors*; D013373:Substance P / Q000037:antagonists & inhibitors; D013844:Thiazoles / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects / Q000378:metabolism; D005719:gamma-Globulins / Q000494:pharmacology
D001549:Benzamides; D044482:Benzeneacetamides; D001713:Biphenyl Compounds; D006634:Histamine H1 Antagonists; D020024:Leukotriene Antagonists; D064729:Neurokinin-1 Receptor Antagonists; D009479:Neuropeptides; D010880:Piperidines; D018041:Receptors, Neurokinin-2; D013844:Thiazoles; C081938:benzylpenicilloyl-bovine-gamma-globulin; D005719:gamma-Globulins; C077265:MCI 826; D013373:Substance P; C073839:SR 48968; D011738:Pyrilamine; D010400:Penicillin G; C066892:CP 96345