title: Cell-cell coupling in cultures of striatal and cortical astrocytes of the monkey Cebus apella.
abstract: Astrocytes were cultured from striatum and neocortex of fetal (embryonic day 90) monkeys (Cebus apella). The cultures grew well, and the cells retained viability after freeze-storage and thawing. The cells displayed depolarized membrane potentials (-19 and -33 mV, for striatal and cortical cells, respectively) and the vast majority of cells were dye-coupled to a mean of 7 (1-18) neighbouring cells. Cell coupling was blocked by octanol (0.25 and 0.5 mM) but was independent of high K+ (10 and 50 mM) and glutamate (500 microm). Thus, cultures of fetal primate astrocytic cells are established as a model system for studies on astroglial cell-cell coupling.
authors.affiliation: Programa Unidad de Neurobiología Aplicada (PRUNA) (CONICET), Buenos Aires, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Gayol
authors.forename: T
authors.initials: T
authors.lastname: Pannicke
authors.forename: A
authors.initials: A
authors.lastname: Reichenbach
authors.forename: J A
authors.initials: JA
authors.lastname: Colombo
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002428:Cebus; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000032:analysis; D017629:Gap Junctions / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology; D007150:Immunohistochemistry; D007546:Isoquinolines / Q000032:analysis; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D019579:Neocortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000442:Octanols / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000494:pharmacology; D014746:Vimentin / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D005904:Glial Fibrillary Acidic Protein; D007546:Isoquinolines; D000442:Octanols; D014746:Vimentin; D018698:Glutamic Acid; C017475:lucifer yellow; D011188:Potassium