Sperm cryopreservation with empty human or mouse zona pellucidae. A comparison.
OBJECTIVE
To compare empty zona pellucidae (ZP) and human of mouse oocytes for sperm cryopreservation.


METHODS
ZP was prepared by evacuating the cytoplasm of human or mouse oocytes. The evacuated ZP were injected with spermatozoa from patients with severe oligoasthenozoospermia from normally fertile men. After a freezing-thawing procedure, the spermatozoa were aspirated outside the ZP. According to the different origins of ZP and sperm, the spermatozoa number per ZP, motile sperm number before freezing and after thawing, lost sperm number per ZP after freezing and sperm recovery rate were compared.


RESULTS
Spermatozoa number, motile sperm number before freezing and after thawing, lost sperm number per ZP and sperm recovery rate in all groups were comparable. The total mean motile sperm number before freezing and after thawing, mean lost sperm number after thawing and sperm recovery rate were 15.1%, 12%, 1.6% and 80%, respectively.


CONCLUSIONS
ZP is an ideal vehicle for cryopreservation of sperm collected from patients with severe oligoasthenozoospermia. There were no differences between human and mouse ZP for sperm storage.
Hsieh
Y Y
YY
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
Tsai
H D
HD
Chang
C C
CC
Lo
H Y
HY
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D015044:Zona Pellucida / Q000502:physiology*