The diagnostic accuracy of microbiologic root canal sampling and the influence of antimicrobial dressings.
The routine approach to endodontic treatment of teeth with apical periodontitis often involves an interappointment dressing with calcium hydroxide. However, investigations have demonstrated a negative influence of calcium hydroxide on the accuracy of microbiological root canal sampling (MRS). The aim of the present study was to investigate whether the use of a fluid dressing like 5% iodine potassium iodide (IPI) would increase the accuracy of MRS. Following instrumentation of 50 teeth with radiographically verified apical periodontitis the root canals received IPI as an intracanal dressing. One week after closure canals were sampled, "test sample" (TS), and then left filled with sampling fluid and temporarily scaled. Seven days later a "gold standard" (GS) sample was obtained. Bacteria were recovered in 22 teeth (44%) in TS as well as in GS. Fifteen teeth (30%) were positive for growth in both samples. Using the detection level "very sparse growth" of microbes the sensitivity and specificity of MRS reached 68% and 75%, respectively. In an earlier study, following the same experimental protocol, but with calcium hydroxide as intracanal dressing, the corresponding values were 33% and 81%. In 25% of these cases bacteria persisted in the canals. As compared to calcium hydroxide, the use of IPI resulted in improved test accuracy, but loss of antibacterial capacity. Conclusively, intracanal dressings seem to vary in their influence on the microbiologic test performance as well as in their antibacterial efficacy. In a clinical situation the choice of interappointment dressing should include consideration of these potentially conflicting properties.
Reit
C
C
Department of Endodontology/Oral Diagnosis, Faculty of Odontology, Göteborg University, Sweden.
Molander
A
A
Dahlén
G
G
D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis; D002126:Calcium Hydroxide / Q000494:pharmacology; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007454:Iodides / Q000494:pharmacology; D008875:Middle Aged; D010485:Periapical Periodontitis / Q000382:microbiology*; D011193:Potassium Iodide / Q000494:pharmacology; D015203:Reproducibility of Results; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D007454:Iodides; D012388:Root Canal Irrigants; D011193:Potassium Iodide; D002126:Calcium Hydroxide; C010389:Lugol's solution