title: Ordered intracellular RecA-DNA assemblies: a potential site of in vivo RecA-mediated activities.
abstract: The inducible SOS response increases the ability of bacteria to cope with DNA damage through various DNA repair processes in which the RecA protein plays a central role. Here we present the first study of the morphological aspects that accompany the SOS response in Escherichia coli. We find that induction of the SOS system in wild-type bacteria results in a fast and massive intracellular coaggregation of RecA and DNA into a lateral macroscopic assembly. The coaggregates comprise substantial portions of both the cellular RecA and the DNA complement. The structural features of the coaggregates and their relation to in vitro RecA-DNA networks, as well as morphological studies of strains carrying RecA mutants, are all consistent with the possibility that the intracellular assemblies represent a functional entity in which RecA-mediated DNA repair and protection activities occur.
authors.affiliation: Department of Organic Chemistry, and Electron Microscopy Center, Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Levin-Zaidman
authors.forename: D
authors.initials: D
authors.lastname: Frenkiel-Krispin
authors.forename: E
authors.initials: E
authors.lastname: Shimoni
authors.forename: I
authors.initials: I
authors.lastname: Sabanay
authors.forename: S G
authors.initials: SG
authors.lastname: Wolf
authors.forename: A
authors.initials: A
authors.lastname: Minsky
mesh_terms: D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics*; D011938:Rec A Recombinases / Q000737:chemistry*; D013014:SOS Response, Genetics
chemical_list: D011938:Rec A Recombinases