title: Schwann cell remyelination is restricted to astrocyte-deficient areas after transplantation into demyelinated adult rat brain.
abstract: The ability to generate large numbers of Schwann cells from a peripheral nerve biopsy makes them potential candidates for the clinical application of cell transplantation to enhance remyelination in human demyelinating disease. Transplant-derived Schwann cell remyelination has previously been demonstrated in the spinal cord but not for demyelinated axons in the brain, a more likely site for initial clinical intervention. We have transplanted Schwann cells from male neonatal rat sciatic nerves into ethidium bromide-induced areas of demyelination in the deep cerebellar white matter of adult female rats. The extent of Schwann cell remyelination 28 days after transplantation was significantly increased in lesions that received direct injections of Schwann cells compared with non-transplanted lesions. Using in situ hybridisation to identify the rat Y chromosome, transplanted male cells were found to co-localise with the P0 immunoreactive area of Schwann cell remyelination. Combined immunohistochemistry and in situ hybridisation confirmed that many remyelinating Schwann cells were transplant-derived. P0 immunoreactivity and transplanted male cells were found in GFAP-negative, astrocyte-free areas. Transplanted Schwann cells were not identified outside of transplanted lesions, nor did they did not contribute to remyelination of a lesion at a distance from the site of transplantation. Our findings indicate that demyelinated axons in the adult brain can be remyelinated by transplanted Schwann cells but that migration and remyelination are restricted to areas from which astrocytes are absent.
authors.affiliation: Department of Clinical Veterinary Medicine, University of Cambridge, United Kingdom.
authors.forename: S A
authors.initials: SA
authors.lastname: Shields
authors.forename: W F
authors.initials: WF
authors.lastname: Blakemore
authors.forename: R J
authors.initials: RJ
authors.lastname: Franklin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism*; D001921:Brain / Q000473:pathology* / Q000503:physiopathology* / Q000648:ultrastructure; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D002531:Cerebellum / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure; D003711:Demyelinating Diseases / Q000139:chemically induced* / Q000473:pathology / Q000628:therapy*; D003714:Denervation; D005260:Female; D008297:Male; D009186:Myelin Sheath / Q000473:pathology* / Q000648:ultrastructure; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D011919:Rats, Inbred Strains; D012038:Regeneration / Q000502:physiology*; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation*; D012737:Sex Factors; D014998:Y Chromosome / Q000235:genetics