Structural characterization and transcriptional pattern of two types of carp rhodopsin gene.
This work characterizes the genomic structures of two types of carp (Cyprinus carpio) rhodopsin (cRh) gene, i.e. type I (cRh-I) and type II (cRh-II). Two types of cRh gene share only 45.6% polynucleotide identity in the upstream region from nucleotide -3436 to +97. However, three conserved regions are found. Homologies to the consensus recognition sites for transcription factors, Crx and Nrl, which are involved in photoreceptor-specific expression, are also observed in cRh genes. With specific polymerase chain reaction (PCR) primers, the two types of cRh gene can be clearly discriminated from each carp genome. Most carps exhibit both types of cRh gene, however, there are still carps possessing either cRh-I or cRh-II. Both cRh-I and cRh-II mRNAs are expressed at an approximately equal level in both eyes extracted from a carp carrying both types of cRh gene.
Su
C Y
CY
Institute of Fisheries Science, National Taiwan University, Taipei, ROC.
Lim
J
J
Tsai
H J
HJ
D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics*; D008969:Molecular Sequence Data; D012243:Rhodopsin / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis; D014158:Transcription, Genetic
D012243:Rhodopsin