[The use of the fluorimetric method for studying DNA integrity exemplified by an assessment of dioxidine genotoxicity].
The genotoxic effect of dioxidine (300, 100, 30, and 10 mg/kg, i.p.) on the liver cells of outbred male rats was studied by fluorometric analysis of DNA unwinding (FADU) using the cell DNA samples taken from animals sacrificed 1, 6, 18, and 24 h after drug introduction. At a dose of 10 mg/kg, dioxidine produced no statistically significant increase in the amount of DNA damage relative to control. Increase in the dioxidine dose to 30 mg/kg resulted in a significant DNA damage 1 h after drug administration, while a dose of 100 or 300 mg/kg reliably caused damage at all times of exposure.
Usol'tsev
M V
MV
Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow, Russia.
Durnev
A D
AD
Seredenin
S B
SB
D000818:Animals; D004247:DNA / Q000032:analysis / Q000187:drug effects* / Q000235:genetics; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005470:Fluorometry / Q000379:methods* / Q000706:statistics & numerical data; D005796:Genes / Q000187:drug effects*; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D011810:Quinoxalines / Q000633:toxicity*; D051381:Rats; D013997:Time Factors
D009153:Mutagens; D011810:Quinoxalines; C001077:dioxidine; D004247:DNA