Potential relevance of Chlamydia pneumoniae surface proteins to an effective vaccine.
The surface of Chlamydia pneumoniae is covered with proteins but their exact identification is not known probably because of the presence of conformational epitopes. A family of 21 pmp genes has been found by DNA sequencing. In common, these genes have the capacity to encode the amino acid motif GGAI. Several of the genes have the capacity to encode outer membrane proteins of about 100 kDa. Thus, they are candidate genes to encode the protein(s) present in the 98-kDa protein band of the C. pneumoniae outer membrane complex. The production of recombinant GGAI proteins is described as is the use of polyclonal antibodies raised against the recombinant GGAI proteins to determine their expression in C. pneumoniae elementary bodies. At least three of the proteins, Omp4, 5, and 11, are expressed.
Christiansen
G
G
Dept. of Medical Microbiology and Immunology, University of Aarhus, DK 8000 Aarhus C, Denmark. gunna@medmicro.au.dk
Pedersen
A S
AS
Hjerno
K
K
Vandahl
B
B
Birkelund
S
S
D020816:Amino Acid Motifs / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology*; D002460:Cell Line; D002690:Chlamydia Infections / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D011994:Recombinant Proteins