Novel anionic microparticles are a potent adjuvant for the induction of cytotoxic T lymphocytes against recombinant p55 gag from HIV-1.
Microparticles with entrapped antigens have recently been shown to possess significant potential as vaccine delivery systems and adjuvants. However, the potential of microparticles as adjuvants has been seriously limited by the common problem of degradation and denaturation of antigens following encapsulation and release. To overcome these problems, we have developed a novel way to use microparticles as adjuvants, by the adsorption of proteins onto their surface. Anionic microparticles were prepared through the inclusion of an anionic detergent, sodium dodecyl sulphate (SDS), in the microparticle preparation process. The anionic microparticles were capable of the efficient and reproducible adsorption of recombinant p55 gag protein from HIV-1. Microparticles with adsorbed p55 were capable of inducing potent cytotoxic T lymphocyte responses in mice following intramuscular immunization. In addition, the microparticles also exhibited a potent adjuvant effect for antibody induction against p55.
Kazzaz
J
J
Chiron Corporation 4560 Horton St., Emeryville, CA 94608, USA.
Neidleman
J
J
Singh
M
M
Ott
G
G
O'Hagan
D T
DT
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000327:Adsorption; D000818:Animals; D000838:Anions; D002460:Cell Line; D005260:Female; D005347:Fibroblasts; D015683:Gene Products, gag / Q000276:immunology*; D007273:Injections, Intramuscular; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008863:Microspheres; D010316:Particle Size; D011498:Protein Precursors / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D012967:Sodium Dodecyl Sulfate / Q000032:analysis; D013501:Surface-Active Agents / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*; D013816:Thermodynamics
D000276:Adjuvants, Immunologic; D000838:Anions; D015683:Gene Products, gag; D011498:Protein Precursors; D011994:Recombinant Proteins; D013501:Surface-Active Agents; C059767:p55 gag precursor protein, Human immunodeficiency virus 1; D012967:Sodium Dodecyl Sulfate