title: cRel-TD kinase: a serine/threonine kinase binding in vivo and in vitro c-Rel and phosphorylating its transactivation domain.
abstract: The activity of transcription factors is often modulated by signal responsive protein kinases. Rel/NF-kappaB transcription factors are regulated by IkappaB inhibitors, the phosphorylation of which causes ubiquitination and degradation, resulting in nuclear translocation of NF-kappaB and activation of target genes. Here we report pulldown and immunoprecipitation experiments showing that a mammalian 66 kDa protein kinase binds murine c-Rel, both in vitro and in vivo. This kinase appears to have at least two binding sites on c-Rel, a proline-directed serine/ threonine substrate specificity similar to MAP kinases and to specifically phosphorylate the C-terminal domain of murine c-Rel at an ERK consensus site.
authors.affiliation: Department of Molecular Pathology and Medicine, Università Vita-Salute San Raffaele, Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Fognani
authors.forename: R
authors.initials: R
authors.lastname: Rondi
authors.forename: A
authors.initials: A
authors.lastname: Romano
authors.forename: F
authors.initials: F
authors.lastname: Blasi
mesh_terms: D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism*; D020569:Proto-Oncogene Proteins c-rel / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D015533:Transcriptional Activation
chemical_list: D016328:NF-kappa B; D020569:Proto-Oncogene Proteins c-rel; D011993:Recombinant Fusion Proteins; D017346:Protein Serine-Threonine Kinases