Inhibition of monkey brain semicarbazide-sensitive amine oxidase by various antidepressants.
We examined whether the antidepressant drugs, such as the dicyclic drug zimeldine, the tricyclic drug imipramine, tetracyclic drug maprotiline, and the non-cyclic drug nomifensine, inhibit in vitro semicarbazide-sensitive amine oxidase (SSAO) activity in monkey brain. The deamination of 1 microM benzylamine was not inhibited at high concentrations of clorgyline or deprenyl, while it was highly sensitive for semicarbazide. When corresponding experiments were performed with 100 microM benzylamine, the opposite results were obtained. The most potent of inhibition of SSAO was observed by imipramine, followed by maprotiline, zimeldine and nomifensine. Inhibition of SSAO was not enhanced by varying the time of preincubation of the enzyme and various antidepressant drugs, indicating direct action on and reversible inhibition of SSAO. We found the tricyclic antidepressant drug to be the most selective inhibitors of SSAO activity in monkey brain, as compared with other type of antidepressant drugs.
Obata
T
T
Department of Pharmacology, Oita Medical University, Hasama-machi, Oita, 879-5593, Japan. tobata@oita-med.ac.jp
Yamanaka
Y
Y
D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology; D006631:Amine Oxidase (Copper-Containing) / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000201:enzymology*; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007099:Imipramine / Q000494:pharmacology; D008251:Macaca; D008297:Male; D008376:Maprotiline / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology*; D009627:Nomifensine / Q000494:pharmacology; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology; D013997:Time Factors; D015031:Zimeldine / Q000494:pharmacology
D018759:Adrenergic Uptake Inhibitors; D006631:Amine Oxidase (Copper-Containing); D000928:Antidepressive Agents; D018765:Dopamine Uptake Inhibitors; D007099:Imipramine; D008376:Maprotiline; D009627:Nomifensine; D017367:Selective Serotonin Reuptake Inhibitors; D015031:Zimeldine