H-2D(b-/-) mice are susceptible to persistent infection by Theiler's virus.
H-2(b) mice are resistant to persistent infection of the central nervous system by Theiler's virus. They clear the infection 7 to 10 days after intracranial inoculation. Resistance maps to the H-2D gene and not to the H-2K gene and is associated with a potent antiviral cytotoxic T-lymphocyte (CTL) response. We used H-2(b) mice in which the H-2D or the H-2K gene had been inactivated to dissect the respective roles of these genes in resistance. We report that H-2D(-/-) but not H-2K(-/-) mice were susceptible to persistent infection. Furthermore, whereas H-2K(-/-) mice mounted a vigorous virus-specific CTL response, similar to that of control C57BL/6 mice, the CTL response of H-2D(-/-) mice was nil or minimal. Using target cells transfected with the H-2D(b) or the H-2K(b) gene, we showed that the H-2K-restricted CTL response against the virus was minimal in H-2D(-/-) mice. These results demonstrate that the H-2D(b) and H-2K(b) genes play nonredundant roles in the resistance to this persistent infection.
Azoulay-Cayla
A
A
Unité des Virus Lents, CNRS URA 1930, France.
Dethlefs
S
S
Pérarnau
B
B
Larsson-Sciard
E L
EL
Lemonnier
F A
FA
Brahic
M
M
Bureau
J F
JF
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D018188:Cardiovirus Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D002478:Cells, Cultured; D002490:Central Nervous System / Q000473:pathology / Q000821:virology; D005805:Genes, MHC Class I; D020022:Genetic Predisposition to Disease; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D064532:Histocompatibility Antigen H-2D; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009154:Mutation / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D017945:Theilovirus / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D013997:Time Factors; D014162:Transfection; D014779:Virus Replication
D000956:Antigens, Viral; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D064532:Histocompatibility Antigen H-2D; D012367:RNA, Viral