Pathogenic leishmania secrete antigenically related chitinases which are encoded by a highly conserved gene locus.
Recently, we identified and characterized a single-copy chitinase gene (LdCht1) from Leishmania donovani, a protozoan pathogen of humans. It has been hypothesized that this parasite enzyme plays a critical role in the survival of all Leishmania species within their sandfly vectors and for their transmission to humans. Thus, in the current study, pulse-field gel electrophoresis and Southern hybridization with the LdCht1 gene probe were used to demonstrate that this chitinase gene has been conserved across species lines of various pathogenic Leishmania. Further, immunoprecipitation and enzyme activity assays using an anti-LdCht1-peptide serum were used to show that the chitinases produced and released by this group of parasites possessed both highly conserved antigenic epitopes and enzyme activities. Results of these studies demonstrate that the chitinase gene locus and enzyme activity have been conserved across species lines among this group of human pathogens.
Shakarian
A M
AM
National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 02892-0425, USA.
Dwyer
D M
DM
D000818:Animals; D015139:Blotting, Southern; D002688:Chitinases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D000939:Epitopes / Q000276:immunology; D007891:Leishmania / Q000201:enzymology / Q000235:genetics / Q000472:pathogenicity*; D011233:Precipitin Tests
D000939:Epitopes; D002688:Chitinases