title: Coexistence of intestinal trefoil factor (hITF) and oxytocin in magnocellular neurons in the human hypothalamus.
abstract: Human intestinal trefoil factor hITF, a polypeptide of the P-domain family, was found to occur in hypothalamic neurons. With combined immunofluorescence and immunoperoxidase technique we investigated the coexistence of hITF with the neurohypophysial peptide oxytocin and the associated neurophysin I in sections of the human hypothalamus. In the supraoptic nucleus, 39.2% of magnocellular oxytocinergic perikarya show hITF immunoreactivity. A similar distribution was observed in perivascular hypothalamic oxytocinergic neurons, whereas in the paraventricular nucleus, 99% of the oxytocinergic neurons show hITF coexpression. In the periventricular nucleus (PEV), single, scattered neurons with both immunoreactivities occur. Our findings indicate that hITF and oxytocin are coexpressed in a portion of the magnocellular neurons in the human hypothalamus, and that hITF is among the neurohypophysial peptides.
authors.affiliation: Institut für Anatomie, Anatomie II, Friedrich-Schiller-Universität Jena, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Griepentrog
authors.forename: M
authors.initials: M
authors.lastname: Bauer
authors.forename: C
authors.initials: C
authors.lastname: Hornstein
authors.forename: H
authors.initials: H
authors.lastname: Sauer
authors.forename: G F
authors.initials: GF
authors.lastname: Jirikowski
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005455:Fluorescent Antibody Technique; D006133:Growth Substances / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009077:Mucins; D009124:Muscle Proteins; D009474:Neurons / Q000737:chemistry*; D009479:Neuropeptides; D010121:Oxytocin / Q000032:analysis*; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry* / Q000166:cytology; D010455:Peptides / Q000032:analysis*; D013495:Supraoptic Nucleus / Q000737:chemistry* / Q000166:cytology; D016707:Tissue Fixation; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3
chemical_list: D006133:Growth Substances; D009077:Mucins; D009124:Muscle Proteins; D009479:Neuropeptides; D010455:Peptides; C099037:TFF3 protein, rat; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D010121:Oxytocin