Inhibitory effect of resveratrol on free radical generation in blood platelets.
Resveratrol (3,4',5-trihydroxystilbene), a compound found in many plants, has been shown to prevent coronary heart diseases and to exert a variety of antiinflammatory and anticancerogenic effects. It is effective in lowering the level of serum lipids and in inhibiting platelet aggregation. We evaluated the effect of trans-resveratrol on the production of free radicals in pig blood platelets and showed that resveratrol inhibited the production of different reactive oxygen species (O2*-, H2O2, singlet oxygen and organic radicals) measured by the luminol-dependent chemiluminescence in resting platelets (P < 0.05). Resveratrol inhibited also the generation of radicals in platelets activated by thrombin (P < 0.05). Treatment of platelets with resveratrol at concentrations of 6.25 and 12.5 microg/ml caused a statistically insignificant increase in the production of O2*- in these cells, as measured by reduction of cytochrome c; however, at higher doses (25, 50 and 100 microg/ml) resveratrol distinctly reduced the generation of O2*- in platelets (P < 0.05). We suggest that free radicals play an important role in the reduced reactivity of blood platelets induced by resveratrol.
Olas
B
B
Department of General Biochemistry, Institute of Biochemistry, University of Lódź, Poland. olasb@Biol.Uni.Lodz.PL
Zbikowska
H M
HM
Wachowicz
B
B
Krajewski
T
T
Buczyński
A
A
Magnuszewska
A
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D005609:Free Radicals / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D010100:Oxygen / Q000097:blood; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000077185:Resveratrol; D026082:Singlet Oxygen; D013267:Stilbenes / Q000494:pharmacology*; D013481:Superoxides / Q000097:blood; D013552:Swine; D013917:Thrombin / Q000494:pharmacology
D000975:Antioxidants; D005609:Free Radicals; D017382:Reactive Oxygen Species; D013267:Stilbenes; D013481:Superoxides; D026082:Singlet Oxygen; D006861:Hydrogen Peroxide; D013917:Thrombin; D000077185:Resveratrol; D010100:Oxygen