A new absorbed coating for DNA fragment analysis by capillary electrophoresis.
A fully automated coating procedure was devised based on adsorption of a new polymer, poly(dimethylacrylamide-co-allyl glycidyl ether) onto the capillary surface. The whole procedure takes less than 30 min and does not require the use of organic solvents, viscous solutions, or elevated temperature. The coating is stable even under harsh conditions such as alkaline pH, elevated temperature, and denaturant conditions that destroy most other presently available adsorbed coatings. This new adsorbed coating is highly stable and easy to produce in quantity, making it quite unique, and further making it possible to operate with any DNA sieving matrix. Finally, the above-mentioned properties facilitate coating regeneration by a simple wash with a strongly alkaline solution, thus extending the lifetime of the capillary, a highly desirable property for any coating used in biopolymer separations.
Chiari
M
M
Institute of Biocatalysis and Molecular Recognition, CNR, Milan, Italy. chiari@ico.mi.cnr.it
Cretich
M
M
Horvath
J
J
D000042:Absorption; D000178:Acrylamides; D000180:Acrylic Resins / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D053938:DNA Fragmentation; D019075:Electrophoresis, Capillary / Q000379:methods; D004852:Epoxy Compounds / Q000737:chemistry; D015394:Molecular Structure; D011108:Polymers
D000178:Acrylamides; D000180:Acrylic Resins; D004852:Epoxy Compounds; D011108:Polymers; C413461:poly(dimethylacrylamide-co-allyl glycidyl ether); D004247:DNA; C099046:N,N-dimethylacrylamide; C054361:allyl glycidyl ether