CD8+ cells suppress oil-induced arthritis.
Oil-induced arthritis is a genetically restricted polyarthritis that develops in the DA rat after injection of the mineral oil Freund's incomplete adjuvant. Here, we investigated the role of the potentially disease-limiting cell populations CD8+ T cells, gammadelta T cells, natural killer (NK) cells and NK T cells in inguinal lymph nodes for the development of this adjuvant-induced arthritis. Flow cytometry analysis before and at disease onset revealed a higher proportion of lymph node T cells expressing NKR-P1 in the disease-resistant LEW.1AV1 compared with the disease-susceptible DA strain, suggesting that NK T cells might be disease protective. However, prophylactic in vivo administration of an anti-NKR-P1 MoAb (clone 10/78) did not consistently affect the disease course. The proportion of CD8+ T cells and the ratio CD4+/CD8+ T cells in inguinal lymph nodes did not differ significantly between DA and LEW.1AV1 rats before or at disease onset. Nevertheless, prophylactic in vivo depletion of CD8+ cells by the OX8 MoAb in the DA strain resulted in an earlier disease onset compared with the control group, demonstrating that CD8+ cells regulate arthritis development. In vivo depletion of gammadelta T cells by the V65 MoAb did not alter the disease course, indicating that the disease-suppressive CD8+ cells are alphabeta T cells or NK cells.
Jansson
A M
AM
Department of Medicine, Unit of Rheumatology, Karolinska Institute, Stockholm, Sweden. asa.jansson@cmm.ki.se
Lorentzen
J C
JC
Bucht
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D001168:Arthritis / Q000139:chemically induced* / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D005434:Flow Cytometry; D007694:Killer Cells, Natural / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D009821:Oils; D051381:Rats; D011917:Rats, Inbred Lew; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*
D000911:Antibodies, Monoclonal; D009821:Oils; D016692:Receptors, Antigen, T-Cell, gamma-delta