title: Recombinase-activating gene (RAG) 2-mediated V(D)J recombination is not essential for tumorigenesis in Atm-deficient mice.
abstract: The majority of Atm-deficient mice die of malignant thymic lymphoma by 4-5 mo of age. Cytogenetic abnormalities in these tumors are consistently identified within the Tcr alpha/delta locus, suggesting that tumorigenesis is secondary to aberrant responses to double-stranded DNA breaks that occur during V(D)J recombination. Since V(D)J recombination is a recombinase-activating gene (RAG)-dependent process, we generated Rag2(-/-)Atm(-/-) mice to assess the requirement for RAG-dependent recombination in thymic lymphomagenesis. In contrast to expectation, the data presented here indicate that development of malignant thymic lymphoma in Atm(-/-) mice is not prevented by loss of RAG-2 and thus is not dependent on V(D)J recombination. Malignant thymic lymphomas in Rag2(-/-)Atm(-/-) mice occurred at a lower frequency and with a longer latency as compared with Atm(-/-) mice. Importantly, cytogenetic analysis of these tumors indicated that multiple chromosomal abnormalities occurred in each tumor, but that none of these involved the Tcr alpha/delta locus. Nonmalignant peripheral T cells from TCR-transgenic Rag2(-/-)Atm(-/-) mice also revealed a substantial increase in translocation frequency, suggesting that these translocations are early events in the process of tumorigenesis. These data are consistent with the hypothesis that the major mechanism of tumorigenesis in Atm(-/-) mice is via chromosomal translocations and other abnormalities that are secondary to aberrant responses to double-stranded DNA breaks. Furthermore, these data suggest that V(D)J recombination is a critical, but not essential, event during which Atm-deficient thymocytes are susceptible to developing chromosome aberrations that predispose to malignant transformation.
authors.affiliation: Experimental Immunology Branch, Genetics Department, Biostatistics and Data Management Section, Office of the Director of the Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: L K
authors.initials: LK
authors.lastname: Petiniot
authors.forename: Z
authors.initials: Z
authors.lastname: Weaver
authors.forename: C
authors.initials: C
authors.lastname: Barlow
authors.forename: R
authors.initials: R
authors.lastname: Shen
authors.forename: M
authors.initials: M
authors.lastname: Eckhaus
authors.forename: S M
authors.initials: SM
authors.lastname: Steinberg
authors.forename: T
authors.initials: T
authors.lastname: Ried
authors.forename: A
authors.initials: A
authors.lastname: Wynshaw-Boris
authors.forename: R J
authors.initials: RJ
authors.lastname: Hodes
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins; D002869:Chromosome Aberrations; D004268:DNA-Binding Proteins; D005434:Flow Cytometry; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008223:Lymphoma / Q000209:etiology*; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency* / Q000502:physiology; D011995:Recombination, Genetic; D013953:Thymus Neoplasms / Q000209:etiology; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; C499308:Rag2 protein, mouse; D025521:Tumor Suppressor Proteins; C064655:V(D)J recombination activating protein 2; D064007:Ataxia Telangiectasia Mutated Proteins; C576325:Atm protein, mouse; D017346:Protein Serine-Threonine Kinases