title: Mice with a selective deletion of the CC chemokine receptors 5 or 2 are protected from dextran sodium sulfate-mediated colitis: lack of CC chemokine receptor 5 expression results in a NK1.1+ lymphocyte-associated Th2-type immune response in the intestine.
abstract: The chemokine receptors CCR2 and CCR5 and their respective ligands regulate leukocyte chemotaxis and activation. To determine the role of these chemokine receptors in the regulation of the intestinal immune response, we induced colitis in CCR2- and CCR5-deficient mice by continuous oral administration of dextran sodium sulfate (DSS). Both CCR2- and CCR5-deficient mice were susceptible to DSS-induced intestinal inflammation. The lack of CCR2 or CCR5 did not reduce the DSS-induced migration of macrophages into the colonic lamina propria. However, both CCR5-deficient mice and, to a lesser degree, CCR2-deficient mice were protected from DSS-induced intestinal adhesions and mucosal ulcerations. CCR5-deficient mice were characterized by a greater relative infiltration of CD4+ and NK1.1+ lymphocyte in the colonic lamina propria when compared to wild-type and CCR2-deficient mice. In CCR5-deficient mice, mucosal mRNA expression of IL-4, IL-5, and IL-10 was increased, whereas that of IFN-gamma was decreased, corresponding to a Th2 pattern of T cell activation. In CCR2-deficient mice, the infiltration of Th2-type T cells in the lamina propria was absent, but increased levels of IL-10 and decreased levels of IFN-gamma may have down regulated mucosal inflammation. Our data indicate that CCR5 may be critical for the promotion of intestinal Th1-type immune responses in mice.
authors.affiliation: Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.
authors.forename: P G
authors.initials: PG
authors.lastname: Andres
authors.forename: P L
authors.initials: PL
authors.lastname: Beck
authors.forename: E
authors.initials: E
authors.lastname: Mizoguchi
authors.forename: A
authors.initials: A
authors.lastname: Mizoguchi
authors.forename: A K
authors.initials: AK
authors.lastname: Bhan
authors.forename: T
authors.initials: T
authors.lastname: Dawson
authors.forename: W A
authors.initials: WA
authors.lastname: Kuziel
authors.forename: N
authors.initials: N
authors.lastname: Maeda
authors.forename: R P
authors.initials: RP
authors.lastname: MacDermott
authors.forename: D K
authors.initials: DK
authors.lastname: Podolsky
authors.forename: H C
authors.initials: HC
authors.lastname: Reinecker
mesh_terms: D000818:Animals; D000941:Antigens / Q000096:biosynthesis; D000950:Antigens, Ly; D000954:Antigens, Surface; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control; D016207:Cytokines / Q000096:biosynthesis; D016264:Dextran Sulfate / Q000633:toxicity*; D005260:Female; D017353:Gene Deletion; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007694:Killer Cells, Natural / Q000276:immunology*; D037181:Lectins, C-Type; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055651:NK Cell Lectin-Like Receptor Subfamily B; D018375:Neutrophil Activation / Q000276:immunology; D014176:Protein Biosynthesis; D011506:Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: D000941:Antigens; D000950:Antigens, Ly; D000954:Antigens, Surface; C515335:Ccr2 protein, mouse; D018925:Chemokines; D016207:Cytokines; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D012333:RNA, Messenger; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D016264:Dextran Sulfate