title: [Measurement of carotenoids by high pressure liquid chromatography: from difficulties to solutions].
abstract: The measurement of serum carotenoids by HPLC has been largely improved during the last 10 years. However these techniques still require much time and skills, and direct application of published methods is rarely satisfying. We report here the difficulties that we met to transfer some HPLC methods described in the literature to our laboratories. We propose some solution to overcome the problems that we have encountered, our experience will perhaps help out other biologists. We reported also some results obtained in healthy populations.
authors.affiliation: Laboratoire de biochimie B, Hôpital central, CO 24, 54035 Nancy cedex.
authors.forename: J P
authors.initials: JP
authors.lastname: Steghens
authors.forename: B
authors.initials: B
authors.lastname: Lyan
authors.forename: G
authors.initials: G
authors.lastname: Le Moel
authors.forename: C
authors.initials: C
authors.lastname: Galabert
authors.forename: V
authors.initials: V
authors.lastname: Fayol
authors.forename: H
authors.initials: H
authors.lastname: Faure
authors.forename: P
authors.initials: P
authors.lastname: Grolier
authors.forename: N
authors.initials: N
authors.lastname: Cheribi
authors.forename: F
authors.initials: F
authors.lastname: Dubois
authors.forename: F
authors.initials: F
authors.lastname: Nabet
mesh_terms: D002338:Carotenoids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D002338:Carotenoids