title: Voltage- and gamma-aminobutyric acid-activated membrane currents in the human medulloblastoma cell line MHH-MED-3.
abstract: The whole-cell patch clamp technique was used to characterize voltage- and neurotransmitter-activated currents in the medulloblastoma cell line MHH-MED-3 and cells from tissue slices and primary cultures of two medulloblastoma biopsies. These preparations revealed similar electrophysiological properties. All tested cells displayed 4-aminopyridine-sensitive delayed rectifying K(+) currents, gamma-aminobutyric acid(A) receptor-mediated Cl(-) currents and most of them inward rectifier K(+) currents. Transient inward currents were mainly carried by low-voltage activated T-type Ca(2+) channels in MHH-MED-3 cells, and tetrodotoxin-sensitive Na(+) channels in cells from the primary culture. From these characteristics we conclude that medulloblastoma cells share physiological features with developing cerebellar granule cells at an immature stage.
authors.affiliation: Institute of Pathology (Neuropathology), Friedrich Schiller University Jena, Bachstrasse 18, D-07740, Jena, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Codina
authors.forename: R
authors.initials: R
authors.lastname: Kraft
authors.forename: T
authors.initials: T
authors.lastname: Pietsch
authors.forename: M
authors.initials: M
authors.lastname: Prinz
authors.forename: C
authors.initials: C
authors.lastname: Steinhäuser
authors.forename: J
authors.initials: J
authors.lastname: Cervós-Navarro
authors.forename: S
authors.initials: S
authors.lastname: Patt
mesh_terms: D000293:Adolescent; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology*; D002528:Cerebellar Neoplasms / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008527:Medulloblastoma / Q000473:pathology / Q000503:physiopathology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D007473:Ion Channels; D005680:gamma-Aminobutyric Acid