title: Recognition and cleavage of DNA by rebeccamycin- or benzopyridoquinoxaline conjugated of triple helix-forming oligonucleotides.
abstract: Indolocarbazole and benzopyridoquinoxaline derivatives have been shown to have anti-tumor activity and to stimulate DNA topoisomerase I-mediated cleavage. Two indolocarbazole compounds (R-6 and R-95) and one benzopyridoquinoxaline derivative (BPQ(1256)) were covalently attached to the 3'-end of a 16mer triple helix-forming oligonucleotide (TFO). These conjugates bind to DNA with a higher affinity than the unsubstituted oligonucleotides. Furthermore, they induce topoisomerase I-mediated and triplex-directed DNA cleavage in a sequence-specific manner.
authors.affiliation: Laboratoire de Biophysique, UMR 8646 CNRS-Muséum National d'Histoire Naturelle, INSERM U201, Paris, France.
authors.forename: P B
authors.initials: PB
authors.lastname: Arimondo
authors.forename: P
authors.initials: P
authors.lastname: Moreau
authors.forename: A
authors.initials: A
authors.lastname: Boutorine
authors.forename: C
authors.initials: C
authors.lastname: Bailly
authors.forename: M
authors.initials: M
authors.lastname: Prudhomme
authors.forename: J S
authors.initials: JS
authors.lastname: Sun
authors.forename: T
authors.initials: T
authors.lastname: Garestier
authors.forename: C
authors.initials: C
authors.lastname: Hélène
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001483:Base Sequence; D002227:Carbazoles; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D018983:DNA Footprinting; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D006868:Hydrolysis; D007211:Indoles; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000494:pharmacology*; D011725:Pyridines / Q000737:chemistry*; D011810:Quinoxalines / Q000737:chemistry*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002227:Carbazoles; D007211:Indoles; D009841:Oligonucleotides; D011725:Pyridines; D011810:Quinoxalines; C411084:benzopyridoquinoxaline; D004247:DNA; C053339:rebeccamycin; D004264:DNA Topoisomerases, Type I