Flow cytometric evaluation of multidrug-resistance circumvention.
Flow cytometry, through the measurement of P-gp expression and function, is an important tool in the characterization of multidrug resistant cell lines used in pharmacology. Furthermore, as a complement to classical in vitro tests, this technique allows rapid evaluation of the capacity of new modulator agents to reverse the resistance to cytotoxic drugs and contributes a deeper insight into the mechanism of action of these new molecules.
Léonce
S
S
Division Recherches Cancérologie, Institut de Recherches Servier, Suresnes, France.
Pierré
A
A
Atassi
G
G
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism; D002453:Cell Cycle; D004317:Doxorubicin / Q000378:metabolism; D018432:Drug Resistance, Multiple / Q000502:physiology*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D010641:Phenotype; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D004317:Doxorubicin