title: Energy landscapes of biomolecular adhesion and receptor anchoring at interfaces explored with dynamic force spectroscopy.
abstract: Beyond covalent connections within protein and lipid molecules, weak noncovalent interactions between large molecules govern properties of cellular structure and interfacial adhesion in biology. These bonds and structures have limited lifetimes and so will fail under any level of force if pulled on for the right length of time. As such, the strength of interaction is the level of force most likely to disrupt a bond on a particular time scale. For instance, strength is zero on time scales longer than the natural lifetime for spontaneous dissociation. On the other hand, if driven to unbind or change structure on time scales shorter than needed for diffusive relaxation, strength will reach an adiabatic limit set by the maximum gradient in a potential of mean force. Over the enormous span of time scales between spontaneous dissociation and adiabatic detachment, theory predicts that bond breakage under steadily rising force occurs most frequently at a force determined by the rate of loading. Moreover, the continuous plot (spectrum) of strength expressed on a scale of loge(loading rate) provides a map of the prominent barriers traversed in the energy landscape along the force-driven pathway and reveals the differences in energy between barriers. Illustrated with results from recent laboratory measurements, dynamic strength spectra provide a new view into the inner complexity of receptor-ligand interactions and receptor lipid anchoring.
authors.affiliation: Department of Physics, University of British Columbia, Vancouver, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Evans
mesh_terms: D000818:Animals; D001665:Binding Sites; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D008962:Models, Theoretical; D015394:Molecular Structure; D011485:Protein Binding; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D008024:Ligands; D011956:Receptors, Cell Surface