Induction of high level of specific antibody response to the neutralizing epitope ELDKWA on HIV-1 gp41 by peptide-vaccine.
The monoclonal antibody 2F5 recognizing the neutralizing epitope ELDKWA on the C-domain could neutralize 90% of the investigated HIV-1 isolates. Low levels of ELDKWA-epitope-specific antibodies were observed in HIV-1-infected individuals. To induce high levels of antibodies to ELDKW-epitope, C-domain peptide (P2) was conjugated with a carrier peptide (KGGG)(7)-K (K/G). P2-K/G-conjugate induced high level of antibodies in mice by titer 1:25,600 to ELDKWA-epitope. P2-K/G-BSA-conjugate induced antibody response to ELDKWA-epitope (1:320-6400) in mice. The ELDKWA-epitope-specific antibodies of 19.8 and 34.6 microg/per milliliter serum were isolated from two rabbit antiserums (1:25,600). The levels of ELDKWA-epitope-specific antibodies induced in rabbits were greater than 1 microg/ml, a level considered to confer long-term protection. These results demonstrate the potential role of the C-domain peptide of gp41 to develop an effective ELDKWA-based epitope/peptide-vaccine against HIV-1.
Liao
M
M
Laboratory of Immunology, Research Centre of Medical Science and the School of Life Science and Engineering, Tsinghua University, 100084, Beijing, Peoples Republic of China.
Lu
Y
Y
Xiao
Y
Y
Dierich
M P
MP
Chen
Y
Y
D016915:AIDS Vaccines / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D000939:Epitopes; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011817:Rabbits; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology
D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal; D000939:Epitopes; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; D014614:Vaccines, Synthetic