Decreased GATA3 mRNA expression in human T-cell lymphotropic virus type 1 (HTLV-1) infection.
GATA3 is a specific T-cell transcription factor involved in the expression of T-cell receptor (TCR). In order to characterize the relationship between HTLV-1 infection, which has been reported to be associated with down-regulation of genes belonging to the TCR/CD3 complex, and the transcription factor GATA3, we evaluated, by semi-quantitative RT-PCR, the expression of GATA3 gene in HTLV-1 carriers and individuals with related diseases. The study included 4 asymptomatic carriers, 2 patients with adult T-cell leukaemia/lymphoma (ATLL), 1 patient with HTLV-1 associated myelopathy (HAM)/tropical spastic paraparesis (TSP) and 7 healthy blood donors. A considerable decrease in the expression of the GATA3 mRNA was observed in all subjects infected by HTLV-1 and no expression of GATA3 mRNA was observed in 1 subject with ATLL and in 1 with HAM/TSP.
Gilli
S C
SC
Hemocentro and Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade de Campinas, São Paulo, Brazil.
Salles
T S
TS
Saad
S T
ST
D001782:Blood Donors; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D050990:GATA3 Transcription Factor; D005786:Gene Expression Regulation; D015490:HTLV-I Infections / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000096:biosynthesis* / Q000235:genetics
D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D012333:RNA, Messenger; D015534:Trans-Activators