A study of proteases throughout the life cycle of Trichinella spiralis.
In the present report we study the proteolytic activity of the excretion-secretion and crude extracts of different stages of Trichinella spiralis (Owen, 1835) Railliet, 1895, (muscle-stage larvae, adult worms before and after mating, and newborn larvae) using natural substrates (structural and hematic mammalian proteins). The analysis of the results allow us to set up a certain stage-specificity, as well as an important relationship between the protease patterns throughout the parasite life cycle and how the parasite may overcome both mechanical and humoral barriers within the host. Muscle-stage larvae present a great activity against structural proteins (collagen), while newborn larvae and adult worms degrade principally hematic proteins (hemoglobin, fibrinogen and immunoglobulin G).
Ros-Moreno
R M
RM
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá, Madrid, Spain.
Vázquez-López
C
C
Giménez-Pardo
C
C
de Armas-Serra
C
C
Rodríguez-Caabeiro
F
F
D000818:Animals; D003094:Collagen / Q000378:metabolism; D004586:Electrophoresis; D010450:Endopeptidases / Q000032:analysis / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D007814:Larva / Q000201:enzymology; D008018:Life Cycle Stages; D013053:Spectrophotometry; D017160:Trichinella spiralis / Q000201:enzymology* / Q000254:growth & development*
D006454:Hemoglobins; D007136:Immunoglobulins; D005340:Fibrinogen; D003094:Collagen; D010450:Endopeptidases