Assessment of in vivo oxidative stress in hypertensive rats and hypertensive subjects in Tanzania, Africa.
Oxidative stress has been reported to be involved in not only cardiovascular diseases but in hypertension, which is a major risk for cardiovascular diseases. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been recognized as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. In the present study, we assessed the oxidative stress in human subjects with hypertension and in hypertensive rats. In stroke-prone spontaneously hypertensive rats at the age of 14 weeks, the excretion of urinary 8-OHdG was significantly (p < 0.05) increased compared with that in age-matched normotensive Wistar-Kyoto rats. Next, we investigated the relationship between oxidative DNA damage and cardiovascular risk factors among Tanzanians aged 46-58 years in a population study carried out in 1998 in at Dar es Salaam, Tanzania, according to the WHO-CARDIAC Study Protocol. Sixty subjects (male/female, 28/32) were selected by SPSS Base 8.0 from those who completed a 24-h urine collection. The 24-h urinary 8-OHdG of the hypertensive subjects (SBP > or =140 mmHg and/or DBP > or =90 mmHg) was significantly (p < 0.05) higher than that of the normotensive subjects (SBP <140 mmHg and DBP <90 mmHg) after adjusting for age and gender (Hypertensives: 17.31 +/- 2.0 ng/mg creatinine, n=38; Normotensives: 10.10 +/- 2.64 ng/mg creatinine, n=22). Oxidative stress was thought to be involved in hypertensive subjects and in hypertensive rats.
Negishi
H
H
Graduate School of Human and Environmental Studies, Kyoto University, Japan.
Njelekela
M
M
Ikeda
K
K
Sagara
M
M
Noguchi
T
T
Kuga
S
S
Kanda
T
T
Liu
L
L
Nara
Y
Y
Tagami
M
M
Yamori
Y
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001794:Blood Pressure; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009929:Organ Size; D018384:Oxidative Stress; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012737:Sex Factors; D013636:Tanzania; D014810:Vitamin E / Q000097:blood
D014810:Vitamin E; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine