title: Estrogen effects on triglyceride metabolism in analbuminemic rats.
abstract: BACKGROUND
  Triglyceride (TG) levels are normally lower in female rats, while the opposite is the case in the Nagase analbuminemic rats (NAR). Increased TG levels in normal males are caused by a testosterone-mediated decrease in postheparin (PH) lipoprotein lipase (LpL). Castration of males reduces TG, while castration of females is without effect. TG levels are reduced by castration of the female NAR, suggesting that estrogen rather than testosterone causes hypertriglyceridemia in this strain. The mechanism for this increase is unknown.
  
  
  METHODS
  We measured secretion of very-low density lipoprotein (VLDL) TG using Triton WR 1339 clearance as the disappearance from blood of 3H-trioleate and 14C-cholesterol-labeled chylomicrons (CM), and the activity of the PH lipases: LpL and hepatic lipase (HL). All were determined in Sprague-Dawley (SD) and NAR female, male, and ovariectomized (OVX) rats.
  
  
  RESULTS
  TG levels were significantly greater in female NAR in comparison to all other groups. Ovariectomy of NAR significantly ameliorated hypertriglyceridemia. VLDL TG secretion was significantly greater in intact female NAR compared with all other groups. There were no other differences in VLDL TG secretion among the other groups. The clearance of CM was greatest in female SD rats, and OVX had no effect. NAR cleared CM less well than did SD rats (P < 0.001), but among NAR, clearance was greatest in OVX NAR and male NAR (P < 0. 002). Both PH LpL activity and HL activity were lowest in female NAR (P < 0.05). Ovariectomy partially corrected the defect in HL (P < 0. 05).
  
  
  CONCLUSIONS
  TG levels in female NAR are in part a result of increased VLDL-TG secretion, an effect mediated by estrogen. The presence of an estrogen-mediated catabolic defect that was alleviated by OVX was also observed. This catabolic defect is likely a result of an estrogen-mediated decrease both in LpL and HL expressed only in the presence of analbuminemia.
authors.affiliation: Division of Nephrology, Department of Medicine UC Davis, Davis, California 95616, USA.
authors.forename: G C
authors.initials: GC
authors.lastname: Shearer
authors.forename: J A
authors.initials: JA
authors.lastname: Joles
authors.forename: H
authors.initials: H
authors.lastname: Jones
authors.forename: R L
authors.initials: RL
authors.lastname: Walzem
authors.forename: G A
authors.initials: GA
authors.lastname: Kaysen
mesh_terms: D000818:Animals; D001769:Blood / Q000378:metabolism; D002914:Chylomicrons / Q000097:blood; D004967:Estrogens / Q000502:physiology*; D005260:Female; D006493:Heparin / Q000494:pharmacology; D008049:Lipase / Q000097:blood; D008071:Lipoprotein Lipase / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D010052:Ovariectomy; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000032:analysis*; D014280:Triglycerides / Q000097:blood*
chemical_list: D002914:Chylomicrons; D004967:Estrogens; D008079:Lipoproteins, VLDL; D012709:Serum Albumin; D014280:Triglycerides; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase