title: The frequency and proliferative potential of megakaryocytic colony-forming cells (Meg-CFC) in cord blood, cytokine-mobilized peripheral blood and bone marrow, and their correlation with total CFC numbers: implications for the quantitation of Meg-CFC to predict platelet engraftment following cord blood transplantation.
abstract: CFC numbers have shown to correlate with success of engraftment and speed of neutrophil recovery following cord blood (CB) transplantation. To investigate whether the number of Meg-CFC in a CB stem cell preparation might correlate with time to platelet engraftment, we evaluated the frequency of Meg-CFC among all CFC types in 134 CB, 21 adult bone marrow (BM) and 52 cytokine-mobilized peripheral blood (PB) stem cell preparations. The correlation of Meg-CFC with the total number of CFC and mixed cell-CFC was also assessed. The frequency of Meg-CFC was highest in CB and correlated significantly with total CFC numbers (mean 20.8%, correlation coefficient (r) 0.84, P = 0.0001) compared with Meg-CFC from mobilized PB (mean 13.1%, r = 0.29, P = 0.07) and BM (mean 4%, r = 0. 39, P = 0.13). In addition, mixed-cell CFC numbers in CB were highly correlated with the total number of Meg-CFC (r = 0.7, P = 0.0001). No such correlations were found with mobilized PB or BM. We conclude that, based on the high degree of correlation between Meg-CFC and non-Meg-CFC numbers in CB, no additional information concerning time to platelet engraftment would be gained by routinely performing Meg-CFC assays in addition to non-Meg-CFC assays. The fact that CB Meg-CFC and mixed-cell CFC are strongly correlated suggests that CB Meg-CFC are more primitive than their counterparts in BM and PB and may reflect the number of stem cells in CB. Bone Marrow Transplantation (2000).
authors.affiliation: The Blood Research Institute of the Blood Center of Southeastern Wisconsin, Milwaukee, WI 53201-2178, USA.
authors.forename: A
authors.initials: A
authors.lastname: Drygalski
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: D
authors.initials: D
authors.lastname: Constantinescu
authors.forename: I
authors.initials: I
authors.lastname: Kashiwakura
authors.forename: T
authors.initials: T
authors.lastname: Farley
authors.forename: L
authors.initials: L
authors.lastname: Dobrila
authors.forename: P
authors.initials: P
authors.lastname: Rubinstein
authors.forename: J W
authors.initials: JW
authors.lastname: Adamson
mesh_terms: D000328:Adult; D001772:Blood Cell Count; D001773:Blood Cells / Q000166:cytology*; D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002452:Cell Count; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005069:Evaluation Studies as Topic; D005312:Fetal Blood / Q000166:cytology*; D006085:Graft Survival; D019650:Hematopoietic Stem Cell Mobilization; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007231:Infant, Newborn; D008533:Megakaryocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology; D009928:Organ Specificity; D013997:Time Factors; D014019:Tissue Donors; D009927:Tissue and Organ Procurement