title: Radioprobing of a RecA-three-stranded DNA complex with iodine 125: evidence for recognition of homology in the major groove of the target duplex.
abstract: A fundamental problem in homologous recombination is how homology between DNAs is recognized. In all current models, a recombination protein loads onto a single strand of DNA and scans another duplex for homology. When homology is found, a synaptic complex is formed, leading to strand exchange and a heteroduplex. A novel technique based on strand cleavage by the Auger radiodecay of iodine 125, allows us to determine the distances between (125)I on the incoming strand and the target sugars of the duplex DNA strands in an Escherichia coli RecA protein-mediated synaptic complex. Analysis of these distances shows that the complex represents a post-strand exchange intermediate in which the heteroduplex is located in the center, while the outgoing strand forms a relatively wide helix intertwined with the heteroduplex and located in its minor groove. The structure implies that homology is recognized in the major groove of the duplex.
authors.affiliation: Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: V A
authors.initials: VA
authors.lastname: Malkov
authors.forename: I G
authors.initials: IG
authors.lastname: Panyutin
authors.forename: R D
authors.initials: RD
authors.lastname: Neumann
authors.forename: V B
authors.initials: VB
authors.lastname: Zhurkin
authors.forename: R D
authors.initials: RD
authors.lastname: Camerini-Otero
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D007457:Iodine Radioisotopes / Q000378:metabolism; D008957:Models, Genetic; D008958:Models, Molecular; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D011938:Rec A Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004277:DNA, Single-Stranded; D007457:Iodine Radioisotopes; D015335:Molecular Probes; D009692:Nucleic Acid Heteroduplexes; C051802:triplex DNA; D004247:DNA; D011938:Rec A Recombinases