Target-cell-induced anergy in natural killer cells: suppression of cytotoxic function.
Our earlier studies have demonstrated that natural killer (NK) cells are the effectors that participate during the spontaneous regression of AK-5 tumour in syngeneic hosts. We have shown that the tumour cells are killed by necrosis and apoptosis. In this study, we have examined the induction of functional anergy in NK cells following coculture with fixed AK-5 tumour cells at high ratio. NK cells, upon coculture with fixed AK-5 cells (1:1 ratio), showed loss of cytotoxic function against both AK-5 (antibody-dependent cell cytotoxicity) as well as YAC-1 targets. The response of these cells to the activation by recombinant interleukin-2 and recombinant interferon gamma was poor. Induction of tumour necrosis factor alpha (TNFalpha) secretion was observed after coculture of NK cells with fixed AK-5 cells. The cocultured cell supernatant inhibited the cytotoxic activity of NK cells, which was partially restored with anti-TNFalpha antibody. In addition, NK cells, after treatment with fixed tumour cells showed overexpression of the Fas receptor. We have also observed induction of apoptosis in cocultured NK cells. These studies suggest that the fixed tumour cells (antigen) at high ratio are able to suppress NK cell function as well as induce death in NK cells.
Das
S
S
Centre for Cellular and Molecular Biology, Hyderabad, India.
Varalakshmi
C
C
Khar
A
A
D000818:Animals; D017209:Apoptosis; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D007108:Immune Tolerance; D007694:Killer Cells, Natural / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D017452:Receptors, IgG / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
D017452:Receptors, IgG; D015375:Receptors, Interleukin-2; D014409:Tumor Necrosis Factor-alpha