Determination of secnidazole in urine by adsorptive stripping voltammetry.
Cyclic voltammetry was used to explore the adsorption behavior of secnidazole on a hanging mercury drop electrode (HMDE). The effects of various operational parameters on the accumulation behavior of the adsorbed species were tested. Thus, a sensitive stripping voltammetry procedure for the determination of secnidazole with an adsorptive accumulation on the surface of HMDE has been developed. Measurements were taken by differential-pulse voltammetry after determination of the optimum conditions. The linear concentration range was 1 x 10(-8)-1 x 10(-7) s when using a 120 s preconcentration at -0.1 V vs. Ag/AgCl in acetate buffer of pH 4.0. The detection limit of secnidazole was 5 x 10(-9) M. The precision, expressed by the coefficient of variation, was 2.5% (n = 10) at a concentration of 1 x 10(-7) m. The method was successfully applied to the analysis of secnidazole in urine.
Radi
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AE
Department of Chemistry, Faculty of Science, Mansoura University, Demiatta, Egypt.
Hassanein
A
A
D000981:Antiprotozoal Agents / Q000652:urine*; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008628:Mercury / Q000737:chemistry; D008795:Metronidazole / Q000031:analogs & derivatives* / Q000652:urine; D010084:Oxidation-Reduction
D000981:Antiprotozoal Agents; D007202:Indicators and Reagents; D008795:Metronidazole; D008628:Mercury; C016724:secnidazole