Quantitative mapping of amplicon structure by array CGH identifies CYP24 as a candidate oncogene.
We show here that quantitative measurement of DNA copy number across amplified regions using array comparative genomic hybridization (CGH) may facilitate oncogene identification by providing precise information on the locations of both amplicon boundaries and amplification maxima. Using this analytical capability, we resolved two regions of amplification within an approximately 2-Mb region of recurrent aberration at 20q13.2 in breast cancer. The putative oncogene ZNF217 (ref. 5) mapped to one peak, and CYP24 (encoding vitamin D 24 hydroxylase), whose overexpression is likely to lead to abrogation of growth control mediated by vitamin D, mapped to the other.
Albertson
D G
DG
[1] Cancer Research Institute, University of California, San Francisco, Box 0808, San Francisco, California, USA. albertson@cc.ucsf.edu
Ylstra
B
B
Segraves
R
R
Collins
C
C
Dairkee
S H
SH
Kowbel
D
D
Kuo
W L
WL
Gray
J W
JW
Pinkel
D
D
D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics*; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005784:Gene Amplification / Q000235:genetics*; D018628:Gene Dosage; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes / Q000235:genetics*; D020161:Physical Chromosome Mapping; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics; D065668:Vitamin D3 24-Hydroxylase
D012333:RNA, Messenger; D015534:Trans-Activators; C113698:ZNF217 protein, human; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase