title: Rapid determination of membrane transport parameters in adherent cells.
abstract: Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials. The method is convenient, cost effective and requires no special apparatus. Initial uptake rates, steady-state and free substrate levels are demonstrated in ZR-75-1 breast cancer and Chinese hamster ovary cell lines using methotrexate, a model agent transported by the reduced folate carrier. The technique should be applicable to the study of the transport properties in a broad range of substrates and cells in monolayer culture.
authors.affiliation: Comprehensive Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Sharif
authors.forename: I D
authors.initials: ID
authors.lastname: Goldman
mesh_terms: D000818:Animals; D001943:Breast Neoplasms; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000502:physiology; D006224:Cricetinae; D015195:Drug Design; D004345:Drug Industry / Q000379:methods*; D005493:Folic Acid Antagonists / Q000493:pharmacokinetics*; D006801:Humans; D008727:Methotrexate / Q000493:pharmacokinetics*; D012588:Scintillation Counting; D014162:Transfection; D014316:Tritium; D014407:Tumor Cells, Cultured
chemical_list: D005493:Folic Acid Antagonists; D014316:Tritium; D008727:Methotrexate