Development of a dye-ligand affinity membrane for the separation of bovine serum albumin.
This study reports the development and testing of a dye-ligand membrane. A synthetic dye called Cibacron Blue F3G-A was utilized as the capturing ligand. Bovine serum albumin acted as the target protein. To immobilize the dye molecules, a microfiltration membrane (Versapor) was used as the supporting matrix. The pore size rating of this membrane was reported to be 0.45 micron. Two different methods of dye immobilization were tested. In the first method, the ligand was directly attached to the membrane, whilst in the second method, a spacer molecule was first coupled to the matrix and then dye coupling was carried out. The chemical stability of the adsorbed dye was studied by subjecting the dye-membranes to appropriate desorption protocols. As a model affinity system, the adsorption of bovine serum albumin onto the dye-membranes was thoroughly investigated. The equilibrium behavior and the kinetic parameters of the BSA-dye interaction were determined experimentally.
Najarian
S
S
Department of Engineering Science, University of Oxford, United Kingdom. najarian@cic.aku.ac.ir
Bellhouse
B J
BJ
D000327:Adsorption; D004396:Coloring Agents; D008024:Ligands; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014227:Triazines
D004396:Coloring Agents; D008024:Ligands; D008567:Membranes, Artificial; D014227:Triazines; D012710:Serum Albumin, Bovine; C012842:Cibacron Blue F 3GA