title: Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus.
abstract: High-performance liquid chromatography-electrospray ionization mass spectrometry has been applied to analyze the flavonoids of Huangqi, the roots of Astragalus mongholicus and A. membranaceus. Eight flavonoids were identified as calycosin-7-O-beta-D-glucoside, calycosin-7-O-beta-D-glucoside-6"-O-malonate (2), ononin, (6aR,11aR)-3-hydroxy-9,10-dimethoxypterocarpan-3-O-bet a-D-glucoside, calycosin, (3R)-7,2'-dihydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside, formononetin-7-O-beta-D-glucoside-6"-O-malonate and formononetin by direct comparison with the isolated standards from Huangqi. The existence of (6aR,11aR)-3-hydroxy-9,10-dimethoxypterocarpan, (3R)-7,2'-dihydroxy-3',4'-dimethoxyisoflavan, astrapterocarpanglucoside-6'-O-malonate and astraisoflavanglucoside-6'-O-malonate was detected. This is the first report of flavonoid glycoside malonates in these two Astragalus species, and malonate 2 is a structurally completely identified new compound.
authors.affiliation: Research Laboratory of Natural Products Chemistry, A.M. Todd Botanicals, Eugene, OR 97402, USA. longze.lin@amtbotanicals.com
authors.forename: L Z
authors.initials: LZ
authors.lastname: Lin
authors.forename: X G
authors.initials: XG
authors.lastname: He
authors.forename: M
authors.initials: M
authors.lastname: Lindenmaier
authors.forename: G
authors.initials: G
authors.lastname: Nolan
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: M
authors.initials: M
authors.lastname: Cleary
authors.forename: S X
authors.initials: SX
authors.lastname: Qiu
authors.forename: G A
authors.initials: GA
authors.lastname: Cordell
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D019684:Magnoliopsida / Q000737:chemistry*; D008314:Malonates / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D019685:Rosales / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D008314:Malonates