title: Functional nerve growth factor receptor in von Recklinghausen neurofibromatosis: an immunocytochemical and short-term culture study.
abstract: Immunocytochemistry reveals 75 kDa low affinity type nerve growth factor receptor (NGFR) on the cell membrane of human neurofibroma cells of von Recklinghausen disease in vivo and in vitro. NGF-immunoreactivity is detected in the primary and cultured tumor cells. Growth augmentation of cultured neurofibroma cells by exogenous NGF is also confirmed. Phosphotyrosine-immunoreactivity is demonstrated by immunocytochemistry in the in vivo and in vitro neurofibroma cells suggesting possible phosphorylation of tyrosine residue in the NGFR or a cellular protein downstream of signal transduction through the ligand receptor system. These results indicate human neurofibroma cells possess functional NGFR and the growth is potentiated through the NGF-NGFR system in the paracrine and/or autocrine fashion.
authors.affiliation: Department of Pathology, Fukushima Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hoshi
authors.forename: T
authors.initials: T
authors.lastname: Yamaki
authors.forename: H
authors.initials: H
authors.lastname: Hiraki
authors.forename: T
authors.initials: T
authors.lastname: Natsume
authors.forename: A
authors.initials: A
authors.lastname: Saitoh
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008479:Mediastinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D020932:Nerve Growth Factor / Q000494:pharmacology; D009455:Neurofibroma / Q000378:metabolism* / Q000473:pathology; D009456:Neurofibromatosis 1 / Q000378:metabolism* / Q000473:pathology; D019000:Phosphotyrosine / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism*; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D020800:Receptor, Nerve Growth Factor; D019000:Phosphotyrosine; D020932:Nerve Growth Factor