Segmental analysis for cocaine and metabolites by HPLC in hair of suspected drug overdose cases.
Hair samples of eight postmortem cases were analyzed in segments of 1 to 3 cm for cocaine, benzoylecgonine and cocaethylene. Samples were prepared for analysis by digestion in 0.1 M HCl and subsequent extraction with mixed-mode solid-phase extraction columns. Measurement was made by reversed-phase, narrow-bore HPLC and fluorescence detection using two laboratory-made internal standards. The concentrations were in the region of 0.29-316 ng/mg of hair for cocaine, 0.43-141 ng/mg of hair for benzoylecgonine and 0.93-1.83 ng/mg of hair for cocaethylene. All eight investigated cases had cocaine-positive segments. In six of the cases, all segments were positive, suggesting regular cocaine use and two showed in-between negative segments indicating an interruption or a change of the abuse intensity. The results showed a second, remarkable observation, i.e. enormous concentration differences (factor >150) for both cocaine and benzoylecgonine between the different subjects. Furthermore, interindividual cocaine/benzoylecgonine ratios ranged from 0.02 to 8.43. We believe these observations could in part be attributed to both some of the still existing limitations in the analytical approach(es), especially the mandatory hair washing steps, and in our still too limited knowledge of the hair incorporation processes. Nevertheless, in some cases, segmental analysis proved to be an important tool to distinguish, together with postmortem examination, deadly chronic abuse from single acute drug overdosage.
Clauwaert
K M
KM
Laboratory for Toxicology, Ghent University, Harelbekestraat 72, B-9000, Gent, Belgium.
Van Bocxlaer
J F
JF
Lambert
W E
WE
De Leenheer
A P
AP
D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives / Q000032:analysis* / Q000506:poisoning*; D019970:Cocaine-Related Disorders / Q000175:diagnosis; D062787:Drug Overdose; D005554:Forensic Medicine; D006197:Hair / Q000737:chemistry*; D006801:Humans
C005618:benzoylecgonine; C066444:cocaethylene; D003042:Cocaine