title: Haematopoietic progenitors from umbilical cord blood.
abstract: OBJECTIVE
  The aim of this study is to improve the obstetrician-based cord blood collection system and an efficient recovery of CD34+ haematopoietic progenitor stem cells.
  
  
  METHODS
  CD34+ cells were purified from total blood using a positive selection enrichment method, called Mini-Macs.
  
  
  RESULTS
  The final yield of CD34+ cells we obtained was 10(4) cells/ml, with a CD34+ purity of 99%.
  
  
  CONCLUSIONS
  Our results confirm that, by using this method, it is possible to get a significant stem cell number, thus improving transplanting both peripheral stem cells and umbilical cord ones.
authors.affiliation: Institute of General Pathology, University of Catania, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Todaro
authors.forename: C
authors.initials: C
authors.lastname: Pafumi
authors.forename: G
authors.initials: G
authors.lastname: Pernicone
authors.forename: S
authors.initials: S
authors.lastname: Munda
authors.forename: M R
authors.initials: MR
authors.lastname: Pilastro
authors.forename: A
authors.initials: A
authors.lastname: Russo
authors.forename: M
authors.initials: M
authors.lastname: Farina
authors.forename: A
authors.initials: A
authors.lastname: Messina
mesh_terms: D018952:Antigens, CD34 / Q000097:blood / Q000187:drug effects; D001781:Blood Component Removal / Q000379:methods* / Q000592:standards; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D004921:Erythropoietin / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D010920:Placenta
chemical_list: D018952:Antigens, CD34; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor