Characterization of a granulovirus from the cassava hornworm (Erinnyis ello: Sphingidae).
A Colombian isolate of Erinnyis ello granulovirus (EeGV) was characterized by electron microscopy, restriction endonuclease digestion, and SDS-PAGE. Electron microscopy showed the occlusion bodies to have a morphology typical of granuloviruses. The restriction patterns of DNA from EeGV and the granuloviruses of Trichoplusia ni (TnGV) and Pieris rapae (PrGV) show little or no similarity, indicating little relatedness among these viruses. EeGV was estimated to possess a relatively small genome of 90.5 +/- 0.5 kbp. SDS-PAGE analysis compared the occulsion body and enveloped nucleocapsid proteins of EeGV and TnGV, and the polypeptide patterns also showed little similarity between these viruses. These analyses, as well as comparison of our results to those reported for other granuloviruses, indicate that EeGV represents a new granulovirus isolate.
Finnerty
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CM
Department of Entomology, Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, New York 14853, USA. cmf5@cornell.edu
Li
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Granados
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D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure*; D016678:Genome; D007915:Lepidoptera / Q000821:virology*; D008854:Microscopy, Electron; D000077183:Occlusion Body Matrix Proteins; D015678:Viral Structural Proteins
D000077183:Occlusion Body Matrix Proteins; D015678:Viral Structural Proteins; C000625703:granulin protein, granulovirus