title: Transferrin and prolactin transcytosis in the lactating mammary epithelial cell.
abstract: The mammary epithelial cell ferries constituents originating from blood and from stromal cells, into milk, by transcytosis. Morphological analysis of a membrane marker of transcytosis in the lactating mammary epithelial cell showed that very rapid endocytosis of surface membrane occurs from both the basolateral and the apical side of the cell. In both cases, membrane trafficking between endosomes and the Golgi complex allows communication between the endocytic and the biosynthetic pathways. Transferrin and prolactin are internalized in mammary cells and transported through multivesicular bodies and Golgi stacks. They are released into milk via different types of secretory vesicles, prolactin being carried in secretory vesicles containing casein micelles. Consequences of the intracellular transport of these proteins and physiological benefits for cell function are discussed.
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire, Inra, Jouy-en-Josas, France. ollivier@biotec.jouy.inra.fr
authors.forename: M
authors.initials: M
authors.lastname: Ollivier-Bousquet
mesh_terms: D000818:Animals; D001692:Biological Transport; D003594:Cytoplasmic Granules / Q000502:physiology; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D006056:Golgi Apparatus / Q000502:physiology; D006801:Humans; D007774:Lactation / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000502:physiology*; D008892:Milk / Q000737:chemistry / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism*
chemical_list: D014168:Transferrin; D011388:Prolactin