The epithelial mucin, MUC1, is expressed on resting T lymphocytes and can function as a negative regulator of T cell activation.
MUC1 is a mucinous glycoprotein which is normally expressed on the surface of a variety of epithelia and aberrantly overexpressed on some human tumors. In this report, we demonstrate that the epithelial mucin, MUC1, is expressed on resting human peripheral blood T cells and two leukemia T cell lines, Jurkat and Hut 78. Crosslinking of MUC1 on peripheral blood T cells by plate-bound anti-MUC1 (DF3-P) antibody inhibits cell proliferation, IL-2 and GM-CSF production, and up-regulation of the IL-2 receptor upon anti-CD3 stimulation. Induction of IL-2 production by Jurkat and HUT 78 is also suppressed and cannot be reversed by the addition of anti-CD28 mAb. These findings suggest that MUC1 can be a potent negative regulator for T cell activation at the resting stage.
Chang
J F
JF
Cell Genesys Inc., Foster City, CA 94404, USA.
Zhao
H L
HL
Phillips
J
J
Greenburg
G
G
D018106:CD28 Antigens / Q000378:metabolism; D002460:Cell Line; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D007108:Immune Tolerance; D007152:Immunologic Capping; D007376:Interleukin-2 / Q000096:biosynthesis; D015458:Leukemia, T-Cell / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics*; D018396:Mucin-1 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000302:isolation & purification; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D018106:CD28 Antigens; D007376:Interleukin-2; D018396:Mucin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor