Immunomodulation by recombinant human interleukin-8 and its signal transduction pathways in invertebrate hemocytes.
We report the presence of interleukin (IL)-8-immunoreactive molecules in hemocytes from the mollusc Mytilus galloprovincialis. Functional studies demonstrate that recombinant human (rh)IL-8 provokes conformational changes, induces chemotaxis, and increases bacterial phagocytic activity in hemocytes. rhIL-8 induces cell shape changes via protein kinase A and C pathways. These morphological changes are followed by reorganization of the actin microfilaments. The findings suggest that, as previously reported for other cytokines, IL-8 is well conserved and deeply involved in immune functions from invertebrates to mammals.
Ottaviani
E
E
Department of Animal Biology, University of Modena and Reggio Emilia, Italy. ottaviani.enzo@unimo.it
Franchini
A
A
Malagoli
D
D
Genedani
S
S
D000818:Animals; D049872:Bivalvia; D002633:Chemotaxis; D006434:Hemocytes / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology / Q000378:metabolism*; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction
D016209:Interleukin-8; D011994:Recombinant Proteins