A simple protocol for separation and assay of mu-calpain, m-calpain, and calpastatin from small tissue samples.
Karlsson
J O
JO
Institute of Anatomy and Cell Biology, Göteborg University, Sweden.
D000818:Animals; D000837:Anion Exchange Resins; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002154:Calpain / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D002364:Caseins; D002847:Chromatography, Agarose / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D015853:Cysteine Proteinase Inhibitors / Q000302:isolation & purification*; D004151:Dipeptides; D005450:Fluorescamine; D007527:Isoenzymes / Q000302:isolation & purification; D012117:Resins, Synthetic; D012685:Sepharose / Q000031:analogs & derivatives; D013379:Substrate Specificity
D000837:Anion Exchange Resins; D002135:Calcium-Binding Proteins; D002364:Caseins; D015853:Cysteine Proteinase Inhibitors; D004151:Dipeptides; D007527:Isoenzymes; D012117:Resins, Synthetic; D005450:Fluorescamine; C020927:phenyl-sepharose; C033668:calpastatin; C042855:Mono Q; D012685:Sepharose; D002154:Calpain