Functional nerve growth factor receptor in von Recklinghausen neurofibromatosis: an immunocytochemical and short-term culture study.
Immunocytochemistry reveals 75 kDa low affinity type nerve growth factor receptor (NGFR) on the cell membrane of human neurofibroma cells of von Recklinghausen disease in vivo and in vitro. NGF-immunoreactivity is detected in the primary and cultured tumor cells. Growth augmentation of cultured neurofibroma cells by exogenous NGF is also confirmed. Phosphotyrosine-immunoreactivity is demonstrated by immunocytochemistry in the in vivo and in vitro neurofibroma cells suggesting possible phosphorylation of tyrosine residue in the NGFR or a cellular protein downstream of signal transduction through the ligand receptor system. These results indicate human neurofibroma cells possess functional NGFR and the growth is potentiated through the NGF-NGFR system in the paracrine and/or autocrine fashion.
Hoshi
N
N
Department of Pathology, Fukushima Medical College, Japan.
Yamaki
T
T
Hiraki
H
H
Natsume
T
T
Saitoh
A
A
Watanabe
K
K
Suzuki
T
T
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008479:Mediastinal Neoplasms / Q000378:metabolism* / Q000473:pathology; D020932:Nerve Growth Factor / Q000494:pharmacology; D009455:Neurofibroma / Q000378:metabolism* / Q000473:pathology; D009456:Neurofibromatosis 1 / Q000378:metabolism* / Q000473:pathology; D019000:Phosphotyrosine / Q000378:metabolism; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism*; D012983:Soft Tissue Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D020800:Receptor, Nerve Growth Factor; D019000:Phosphotyrosine; D020932:Nerve Growth Factor