An enzyme immunoassay for detection of Japanese encephalitis virus-induced chemotactic cytokine.
Japanese encephalitis virus (JEV) induces human peripheral blood monocytes to secrete a chemotactic cytokine [human macrophage-derived factor (hMDF)] which causes chemotaxis of neutrophils. The only known assay for hMDF cannot quantify its level in samples, so an enzyme immunoassay has been standardized for detection of hMDF and hMDF-specific antibodies in test samples. The reported enzyme linked immunosorbent assay (ELISA) was found to be sensitive (89%), specific (91%), accurate (92 2%) and reproducible and was able to detect a minimum concentration of 23 ng hMDF/ml in test samples. The chemotactic factor could be detected in JEV inoculated mouse sera and JEV infected culture fluids. Significant finding of the test was the detection of hMDF in sera of human cases of JE.
Singh
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A
Postgraduate Department of Microbiology, KG's Medical College, Lucknow 226 003, India.
Kulshreshtha
R
R
Mathur
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A
D000818:Animals; D054422:Chemokine CCL22; D019742:Chemokines, CC / Q000032:analysis / Q000096:biosynthesis*; D004664:Encephalitis Virus, Japanese; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000378:metabolism / Q000821:virology*; D051379:Mice; D012680:Sensitivity and Specificity
C515482:CCL22 protein, human; C515483:Ccl22 protein, mouse; D054422:Chemokine CCL22; D019742:Chemokines, CC