Oxidative injury is present in Purkinje cells in patients with olivopontocerebellar atrophy.
To verify the presence of lipid peroxidation products in spinocerebellar degeneration (SCD), the cerebella from eight patients with olivopontocerebellar atrophy (OPCA) and six non-OPCA patients were immunohistochemically investigated with 4-hydroxy-2-nonenal (HNE) antibody. On average, 84.6% of Purkinje cells were positively or strongly positively immunostained in OPCA patients while only 15.5% were positive in non-OPCA patients. Other cells in the molecular and granular layers showed no obvious immunoreactivity. These data suggest that a lipid peroxidation product is present in Purkinje cells of OPCA patients and that oxidative stress may play an important role in the degeneration process of SCD.
Yamashita
T
T
Department of Neurology, Kumamoto University School of Medicine, 1-1-1 Honjo, 860-0811, Kumamoto, Japan.
Ando
Y
Y
Obayashi
K
K
Terazaki
H
H
Sakashita
N
N
Uchida
K
K
Ohama
E
E
Ando
M
M
Uchino
M
M
D000368:Aged; D000447:Aldehydes / Q000276:immunology / Q000378:metabolism; D000906:Antibodies; D002531:Cerebellum / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009849:Olivopontocerebellar Atrophies / Q000378:metabolism* / Q000473:pathology*; D018384:Oxidative Stress; D011689:Purkinje Cells / Q000378:metabolism*
D000447:Aldehydes; D000906:Antibodies; C027576:4-hydroxy-2-nonenal