NMDAR1 isoforms in the rat superior olivary complex and changes after unilateral cochlear ablation.
Normal expression and deafness related changes in expression of NMDAR1 isoforms were examined in the rat superior olivary complex (SOC) using in situ hybridization with S35 labeled oligoprobes. Expression was assessed in three SOC nuclei, the lateral and medial superior olives (LSO, MSO) and the medial nucleus of the trapezoid body (MNTB). Silver grain labeling over principal cells of each region was assessed using METAMORPH image analysis system. Counts were made in ipsi- and contralateral sides after unilateral cochlear ablation and in treated and untreated animals. In the normal SOC, NMDAR1a expression was higher than 1b and 1-2 expression was followed by 1-4 and 1-1, with 1-3 below the level for detection. The levels and ratio were comparable in LSO, MSO and MNTB. Five days after cochlear ablation 1a, 1-1, 1-2 and 1-4 showed significant decreases in the ipsilateral LSO and 1-a and 1-2 showed significant decreases in the contralateral MNTB, with no significant changes in the MSO. At 20 days after deafening, no significant changes were seen for any isoform in any nucleus. The transient deafness-induced decreases in expression of NMDAR1 isoforms correlate with loss of excitation.
Nakagawa
H
H
Kresge Hearing Research Institute, University of Michigan, 1301 East Ann St., Ann Arbor, MI 48109-0506, USA.
Sato
K
K
Shiraishi
Y
Y
Kuriyama
H
H
Altschuler
R A
RA
D000818:Animals; D003051:Cochlea / Q000502:physiology* / Q000601:surgery; D003638:Deafness / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D009847:Olivary Nucleus / Q000166:cytology / Q000378:metabolism*; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000032:analysis / Q000235:genetics*; D013997:Time Factors
D020033:Protein Isoforms; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate