D(Va) category phenotype and genotype in Japanese families.
OBJECTIVE
The genetic background of the D(Va) category phenotype has been described in two Caucasian individuals. We were interested in the RHD sequence of 7 Japanese D(Va) individuals and their families.


METHODS
With SSP-PCR we tested exons 4, 5 and 7 of the RHD gene and used restriction enzymes for testing nucleotide associated with the D(Va) phenotype.


RESULTS
A single RHD G667 C697 allele was present in 5 individuals with D(Va) category phenotype, and in 2 individuals we found a D-CE-D hybrid gene (exon 5 had been replaced). The D(Va)Ce gene complex was found in all families.


CONCLUSIONS
The changes of the RHD gene described in European D(Va) individuals were also observed in Japanese families.
Legler
T J
TJ
Department of Transfusion Medicine, University of Göttingen, Germany. tlegler@med.uni-goettingen.de
Wiemann
V
V
Ohto
H
H
Matuda
I
I
Obara
T
T
Uchikawa
M
M
Köhler
M
M
D000483:Alleles; D044466:Asian People / Q000235:genetics*; D005091:Exons; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D010375:Pedigree; D010641:Phenotype; D018807:Polymorphism, Single-Stranded Conformational; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D012698:Serologic Tests
D012204:Rh-Hr Blood-Group System