Expression of IL-2 receptor in dorsal root ganglion neurons and peripheral antinociception.
Interleukin-2 (IL-2), a T-cell growth factor, could exert an antinociceptive effect. Intraplantar injection of human recombinant IL-2 (rIL-2) at a dose of 10000/20 microl significantly enhanced pain threshold as measured by paw withdrawal latency (PWL) to noxious radiant heat in the rat. Reverse transcription modification of polymerase chain reaction (RT-PCR) analysis showed that IL-2R alpha and beta chains were constitutively expressed in rat dorsal root ganglion (DRG) neurons. Immunohistochemistry further demonstrated that IL-2R was mainly localized to small and medium-sized neurons, which are predominantly responsible for nociceptive transmission. These observations suggest that IL-2/IL-2R system may affect neural activities including nociceptive processing in DRG.
Song
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Key Laboratory of Neurobiology, Shanghai Institute of Physiology, Chinese Academy of Sciences, PR China.
Zhao
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ZQ
Liu
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D000818:Animals; D000906:Antibodies; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology*; D017288:Pain Threshold / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000906:Antibodies; D007376:Interleukin-2; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins