Crystallization and preliminary X-ray analysis of the matrix protein from Ebola virus.
The matrix protein from Ebola virus is a membrane-associated molecule that plays a role in viral budding. Despite its functional similarity to other viral matrix proteins, it displays no sequence similarity and hence may have a distinct fold. X-ray diffraction quality crystals of the Ebola VP40 matrix protein were grown by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 64.4, b = 91.1, c = 47.9 A, beta = 96.3 degrees. A data set to 1.9 A resolution has been collected using synchrotron radiation. The unit cell contains one molecule of molecular weight 35 kDa per asymmetric unit, with a corresponding volume solvent content of 35%.
Dessen
A
A
European Molecular Biology Laboratory (EMBL), Grenoble, France.
Forest
E
E
Volchkov
V
V
Dolnik
O
O
Klenk
H D
HD
Weissenhorn
W
W
D003460:Crystallization; D018360:Crystallography, X-Ray; D029043:Ebolavirus / Q000737:chemistry*; D015951:Factor Xa / Q000235:genetics; D005822:Genetic Vectors; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014763:Viral Matrix Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C080981:VP40 protein, virus; D014763:Viral Matrix Proteins; D014764:Viral Proteins; D015951:Factor Xa