Efficacy of ELISA for detection of antibodies against several Ornithobacterium rhinotracheale serotypes.
The present investigation were carried out to compare self-made ELISA based on SDS-Antigen extraction of serotype B and one commercial ELISA-kit (Biocheck, Gouda, The Netherlands) for their ability to detect antibodies against 12 ORT serotypes (A-L). Using both ELISA systems, antibodies against all serotypes were detected. Examination of serum samples collected from commercial flocks showed similar results on flock bases. However, some minor variations on sample basis could be demonstrated. In conclusion using both ELISA system antibodies against different serotypes could be detected.
Hafez
H M
HM
Institute of Poultry Diseases, Free University Berlin, Germany. hafez@zedat.fu-berlin.de
Mazaheri
A
A
Sting
R
R
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012703:Serotyping; D014422:Turkeys
D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic