Characterization of an 84 kDa protein inducing an immediate hypersensitivity reaction in rabbits sensitized to Haemaphysalis longicornis ticks.
An immunogenic 84 kDa protein was isolated and purified from whole tick extracts of Haemaphysalis longicornis larvae by a combination of ion exchange, reverse phase and hydrophobic interaction chromatographies. The protein, when injected intradermally into rabbits exposed to repeated tick feeding, induces an immediate cutaneous hypersensitivity reaction. It has been purified to homogeneity as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis and silver staining. Amino acid sequences for two peptides derived from proteolytic cleavage of p84 were scanned against known proteins on the SWISS-PROT database. A 7 residue motif, ISGWGNT present in one of the two peptides appeared conserved in both vertebrate and invertebrate trypsin-like serine proteinases, while another 7 amino acid motif, HVPAGQI present in the second peptide showed homology to an Escherichia coli ATP-binding protein. We have discussed our findings in relation to isolation and characterization of target antigens for tick vaccine candidates.
Mulenga
A
A
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, 060-0818, Hokkaido, Sapporo, Japan.
Sugimoto
C
C
Ohashi
K
K
Onuma
M
M
D000818:Animals; D002845:Chromatography / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006969:Hypersensitivity, Immediate / Q000209:etiology* / Q000276:immunology; D007279:Injections, Subcutaneous; D019476:Insect Proteins / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D010446:Peptide Fragments / Q000737:chemistry; D011817:Rabbits; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000276:immunology*
D019476:Insect Proteins; D010446:Peptide Fragments