Micropattern immobilization of polysaccharide.
Two types of polysaccharides, sulfated hyaluronic acid and heparin, were pattern-immobilized on a poly(ethylene terephthalate) and polystyrene film, respectively, in a specific pattern by photolithography. Sulfated hyaluronic acid was prepared from hylaronic acid by the treatment of sulfur trioxide/pyridine complex. Heparin was purchased and used without further treatment. The polysaccharides were coupled with azidoaniline. The derivatized polysaccharides were cast on a film from aqueous solution. After drying, the film was photo-irradiated in the presence or absence of a photomask. The micropatterning was confirmed by staining with a cationic dye. Platelet adhesion was reduced on the sulfated hyaluronic acid-immobilized areas. The immobilized sulfated hyaluronic acid significantly reduced thrombus formation. On the other hand, cells were cultured on the heparin-immobilized film. In the presence of fibroblast growth factor (FGF), the growth of mouse fibroblast STO cells was enhanced only on the heparin-immobilized regions. This result indicated that micropattern-immobilized heparin activated FGF for cell growth activity.
Ito
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Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Japan. ito@bio.tokushima-u.ac.jp
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D006820:Hyaluronic Acid / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D010973:Platelet Adhesiveness; D011093:Polyethylene Terephthalates / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D011725:Pyridines; D013461:Sulfur Oxides
D000925:Anticoagulants; D011093:Polyethylene Terephthalates; D011137:Polystyrenes; D011725:Pyridines; D013461:Sulfur Oxides; D005346:Fibroblast Growth Factors; D006820:Hyaluronic Acid; D006493:Heparin; C011118:sulfur trioxide; C023666:pyridine