Cloning and expression analysis of an inducible HSP70 gene from tilapia fish.
We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other HSP70 genes. A dramatic increase of tilapia HSP70 mRNA levels was observed after heat shock of whole animals in all organs tested. Reporter constructs were tested for transient expression in carp cells and in microinjected zebrafish embryos. The entire isolated regulatory region (-851/+157) was able to mediate heat shock inducible expression of the reporter gene, with no preference for a particular tissue. Our studies represent the first transcriptional analysis of a HSP70 promoter from fish, revealing a powerful tool to direct controlled, tissue-independent gene expression in fish.
Molina
A
A
Laboratoire de Biologie Moléculaire et Génie Génétique, Université de Liège, Institut de Chimie B6, B-40000 Sart-Tilman, Belgium, UK.
Biemar
F
F
Müller
F
F
Iyengar
A
A
Prunet
P
P
Maclean
N
N
Martial
J A
JA
Muller
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016385:TATA Box; D017210:Tilapia / Q000235:genetics*; D014162:Transfection; D015027:Zebrafish / Q000196:embryology
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D004247:DNA