Development of an immunochemical technique for the analysis of trichlorophenols using theoretical models.
An immunoassay has been developed for trichlorophenol analysis on the basis of theoretical chemistry modeling studies. These data have allowed us to choose the optimum chemical structure of the immunizing hapten according to realistic similarities with the target analyte. The synthesis of this hapten and the subsequent application of an appropriate immunization protocol have lead to the production of polyclonal antibodies against the target analyte. A homologous direct competitive ELISA has been developed that can be carried out in about 1 h. It has a limit of detection of 0.2 +/- 0.06 microg/L (1.01 +/- 0.3 nM) and it has been proven to tolerate a wide range of ionic strengths and pH values. Thus, the assay has acceptable features in samples with ionic strength between 4 and 56 mS/cm and pH values between 5.5 and 9.5. Studies on the selectivity of this immunoassay have demonstrated a high recognition of the corresponding brominated analogues. Other phenolic compounds do not interfere significantly in the analysis of 2,4,6-trichorophenol using this immunochemical technique. The accuracy of the assay has been evaluated using certified and spiked samples.
Galve
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Department of Biological Organic Chemistry, Institute of Chemistry and Environmental Research of Barcelona Josep Pascual Vila (IIQAB-CSIC), Jorge Girona, Spain.
Camps
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Sanchez-Baeza
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Marco
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MP
D000818:Animals; D001667:Binding, Competitive; D002733:Chlorophenols / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006241:Haptens / Q000276:immunology*; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D007120:Immunochemistry / Q000379:methods; D008958:Models, Molecular; D009994:Osmolar Concentration; D011136:Polysorbates; D011817:Rabbits; D012680:Sensitivity and Specificity; D013997:Time Factors
D002733:Chlorophenols; D006241:Haptens; D011136:Polysorbates; C024564:2,4,6-trichlorophenol