title: Axonal transport of neuronal calcium sensor-1 and phosphatidylinositol 4-kinase beta in the adult rat sciatic nerve.
abstract: Neuronal calcium sensor-1 (NCS-1) and its putative substrate phosphatidylinositol 4-kinase beta (PtdIns 4-kinase beta) both indirectly regulate synaptic vesicle exocytosis and are located in DRG neurites. In this study we have tested whether NCS-1 and PtdIns 4-kinase beta are transported in axons using the analysis of double ligation approach in the adult rat sciatic nerve. We show that NCS-1 accumulates on both the distal and proximal side of the nerve ligation indicating that this protein undergoes bidirectional transport in axons. In contrast, PtdIns 4-kinase beta accumulated on the distal side which suggests that it undergoes retrograde axonal transport and unlike NCS-1 was also present in non-neuronal cells.
authors.affiliation: Division of Neuroscience, The John Curtin School of Medical Research, Australian National University, Canberra, ACT.
authors.forename: A J
authors.initials: AJ
authors.lastname: Reynolds
authors.forename: I A
authors.initials: IA
authors.lastname: Hendry
authors.forename: S E
authors.initials: SE
authors.lastname: Bartlett
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D000918:Antibody Specificity; D001370:Axonal Transport / Q000502:physiology*; D015153:Blotting, Western; D020013:Calcium Signaling / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D008026:Ligation; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000737:chemistry / Q000201:enzymology*
chemical_list: D002135:Calcium-Binding Proteins; D051580:Neuronal Calcium-Sensor Proteins; D009479:Neuropeptides; C101464:frequenin calcium sensor proteins; D019870:1-Phosphatidylinositol 4-Kinase