Haematopoietic progenitors from umbilical cord blood.
OBJECTIVE
The aim of this study is to improve the obstetrician-based cord blood collection system and an efficient recovery of CD34+ haematopoietic progenitor stem cells.


METHODS
CD34+ cells were purified from total blood using a positive selection enrichment method, called Mini-Macs.


RESULTS
The final yield of CD34+ cells we obtained was 10(4) cells/ml, with a CD34+ purity of 99%.


CONCLUSIONS
Our results confirm that, by using this method, it is possible to get a significant stem cell number, thus improving transplanting both peripheral stem cells and umbilical cord ones.
Todaro
A M
AM
Institute of General Pathology, University of Catania, Italy.
Pafumi
C
C
Pernicone
G
G
Munda
S
S
Pilastro
M R
MR
Russo
A
A
Farina
M
M
Messina
A
A
D018952:Antigens, CD34 / Q000097:blood / Q000187:drug effects; D001781:Blood Component Removal / Q000379:methods* / Q000592:standards; D001800:Blood Specimen Collection / Q000379:methods / Q000592:standards; D004921:Erythropoietin / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D010920:Placenta
D018952:Antigens, CD34; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor