Hybridization and breeding of the benomyl resistant mutant, Trichoderma harziantum antagonized to phytopathogenic fungi by protoplast fusion.
A diploid strain obtained from heterokaryons of Trichoderma harzianum by protoplast fusion grew on minimal medium containing 100ppm benomyl. This strain inhibited the growth of the phytopathogenic fungus Fusarium oxysporum f. sp. raphani on paired cultures and also protected against radish yellows and a drop in germination induced by F. oxysporum f. sp. raphani.
Ogawa
K
K
Department of Biological Resource Sciences, Faculty of Agriculture, Miyazaki University, Japan. a04106u@cc.miyazaki-u-ac.jp
Yoshida
N
N
Gesnara
W
W
Omumasaba
C A
CA
Chamuswarng
C
C
D001542:Benomyl / Q000494:pharmacology*; D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics; D005659:Fungicides, Industrial / Q000494:pharmacology*; D005670:Fusarium / Q000254:growth & development; D006824:Hybridization, Genetic; D009154:Mutation; D011523:Protoplasts; D014242:Trichoderma / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*
D003470:Culture Media; D005659:Fungicides, Industrial; D001542:Benomyl