title: A preliminary study of single nucleotide polymorphisms of lipoprotein lipase gene in coronary atherosclerotic heart disease.
abstract: OBJECTIVE
  This study inquired into the relationship between single nucleotide polymorphisms(SNPs) of lipoprotein lipase gene (LPL) and coronary atherosclerotic heart disease(CHD).
  
  
  METHODS
  Exon 6 and fragment of intron 6 in LPL gene from 102 cases of CHD and 110 normal subjects were analysed by polymerase chain reaction amplification, temperature-modulated high-performance liquid chromatography (TmHPLC) and DNA sequencing.
  
  
  RESULTS
  Three new SNPs (4212t/c, 4509t/c, 4576a/c) were detected in the intron 6 of LPL gene. There was significant difference in frequencies of 4212t/c, and 4576a/c between health group and CHD group(P<0.05).
  
  
  CONCLUSIONS
  This study has accumulated additional data on SNPs in LPL gene and provided new data for exploring the mechanism of CHD.
authors.affiliation: Department of Medical Genetics, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P. R. China. zszg@wcums.edu.cn
authors.forename: Z
authors.initials: Z
authors.lastname: Su
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Hou
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Liao
authors.forename: Q
authors.initials: Q
authors.lastname: Xia
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: H
authors.initials: H
authors.lastname: Meng
authors.forename: Y
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013696:Temperature
chemical_list: D008071:Lipoprotein Lipase