Novel intracellular effects of human connective tissue growth factor expressed in Cos-7 cells.
To clarify the multiple functionality of connective tissue growth factor (CTGF), we examined the effects of nascent CTGF within the cell by transient expression. In Cos-7 cells, expression of human CTGF induced an altered cell morphology. It was associated with an increased cellular DNA content and loose attachment, indicating the cells were in G2/M phase. Overexpression of CTGF did not induce cell growth, whereas recombinant CTGF efficiently stimulated the proliferation extracellularly. These results indicate that intracellular CTGF may act as an antimitotic agent, thus it should also be noted that nascent CTGF was found to accumulate around the central mitotic machinery.
Kubota
S
S
Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan.
Hattori
T
T
Shimo
T
T
Nakanishi
T
T
Takigawa
M
M
D000818:Animals; D019556:COS Cells; D002448:Cell Adhesion; D002455:Cell Division; D018385:Centrosome / Q000737:chemistry; D055513:Connective Tissue Growth Factor; D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000494:pharmacology / Q000502:physiology*; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
C526317:CCN2 protein, human; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D055513:Connective Tissue Growth Factor; D004247:DNA