title: Efficient selection for thermostable protease in Thermus thermophilus.
abstract: An efficient procedure was established to select for thermostable proteases in an extreme thermophile, Thermus thermophilus. A non-protease-secreting mutant derived from T. thermophilus TH125 was used as host and the expression plasmid for aqualysin I from T. aquaticus YT-1 was constructed as a source of thermostable protease. T. thermophilus cells harboring the recombinant plasmid produced active aqualysin I into the medium and were able to grow on a minimal medium containing milk casein as the sole source of carbon and nitrogen.
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Japan. hiro@fpu.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Takagi
authors.forename: A
authors.initials: A
authors.lastname: Suzumura
authors.forename: Y
authors.initials: Y
authors.lastname: Hasuura
authors.forename: T
authors.initials: T
authors.lastname: Hoshino
authors.forename: S
authors.initials: S
authors.lastname: Nakamori
mesh_terms: D003470:Culture Media; D004795:Enzyme Stability; D006361:Heating; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D013824:Thermus / Q000201:enzymology / Q000235:genetics; D016963:Thermus thermophilus / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D011993:Recombinant Fusion Proteins; D012697:Serine Endopeptidases; C055102:aqualysin I