title: Antiserum against S-100 protein prevents long term potentiation through a cAMP-related mechanism.
abstract: Long term potentiation (LTP) was induced in the CA1 region of rat hippocampal slices by tetanization of the Schaffer collaterals. Local pretreatment of CA1 with serum of rabbits immunized against S-100 prevented the potentiation. However, treatment of the slices with a membrane permeant cAMP analogue, such as 8-Br-cAMP, could protect against the blocking effect of anti S-100 serum. We suggest that in the rat endogenous S-100b is involved in transduction mechanisms during LTP induction, via its ability to stimulate adenylate cyclase. Possible mechanisms of this action are discussed.
authors.affiliation: Department of Neurological Sciences, University of Genoa, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Rebaudo
authors.forename: R
authors.initials: R
authors.lastname: Melani
authors.forename: M
authors.initials: M
authors.lastname: Balestrino
authors.forename: A
authors.initials: A
authors.lastname: Cupello
authors.forename: K
authors.initials: K
authors.lastname: Haglid
authors.forename: H
authors.initials: H
authors.lastname: Hydèn
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism*; D004558:Electric Stimulation; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000502:physiology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000037:antagonists & inhibitors*
chemical_list: D007106:Immune Sera; D009418:S100 Proteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP