title: ZEITLUPE encodes a novel clock-associated PAS protein from Arabidopsis.
abstract: We have conducted genetic screens for period length mutants in Arabidopsis using a transgenic bioluminescence phenotype. This screen identified mutations at a locus, ZEITLUPE (ZTL), that lengthen the free-running period of clock-controlled gene transcription and cell expansion, and alter the timing of the daylength-dependent transition from vegetative to floral development. Map-based cloning of ZTL identified a novel 609 amino acid polypeptide consisting of an amino-terminal PAS domain, an F box and six carboxy-terminal kelch repeats. The PAS region is highly similar to the PAS domain of the Arabidopsis blue-light receptor NPH1, and the Neurospora circadian-associated protein WHITE COLLAR-1 (WC-1). The striking fluence rate-dependent effect of the ztl mutations suggests that ZTL plays a primary role in the photocontrol of circadian period in higher plants.
authors.affiliation: The Scripps Research Institute, Department of Cell Biology, La Jolla, California 92037, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Somers
authors.forename: T F
authors.initials: TF
authors.lastname: Schultz
authors.forename: M
authors.initials: M
authors.lastname: Milnamow
authors.forename: S A
authors.initials: SA
authors.lastname: Kay
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins; D001483:Base Sequence; D001683:Biological Clocks; D018744:DNA, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D010940:Plant Proteins; C410503:ZTL protein, Arabidopsis