title: Use of the placenta as an artificial lung.
abstract: Oxygen transfer across a single cotyledon of the human placenta was assessed by using three different perfusates in the maternal circuit: 1) M-199 culture medium, 2) human adult red blood cells (RBCs), and 3) perflubron. These maternal circuit perfusates were oxygenated with a membrane oxygenator. RBCs were perfused on the fetal side of the circuit and samples were taken preplacenta and postplacenta for each maternal perfusate. PO2 and PCO2 were measured and O2 transfer was calculated for each maternal perfusate. O2 transfer per single cotyledon (mean +/- SE) was 0.18 +/- 0.04, 0.20 +/- 0.03, and 0.15 +/- 0.05 ccO2/min when using: 1) M-199, 2) RBCs, and 3) perflubron, respectively. O2 transfer per kilogram of placental tissue was 13.08 +/- 2.78, 14.57 +/- 2.05, and 10.43 +/- 3.79 ccO2/kg per minute when using: 1) M-199, 2) RBCs, and 3) perflubron, respectively. When extrapolated to the individual weights of the entire placenta, the O2 transfer was 9.15 +/- 1.95, 10.20 +/- 1.43, and 7.30 +/- 2.65 when using: 1) M-199, 2) RBCs, and 3) perflubron, respectively. We conclude from these data that O2 transfer can be accomplished during placental perfusion. Larger studies are required to differentiate efficacy among the three maternal circuit perfusates.
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor 48109-0245, USA.
authors.forename: S
authors.initials: S
authors.lastname: Sawada
authors.forename: C A
authors.initials: CA
authors.lastname: Reickert
authors.forename: R H
authors.initials: RH
authors.lastname: Bartlett
authors.forename: R B
authors.initials: RB
authors.lastname: Hirschl
mesh_terms: D000328:Adult; D001187:Artificial Organs; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D001802:Blood Substitutes / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism; D015199:Extracorporeal Membrane Oxygenation / Q000379:methods; D005260:Female; D005466:Fluorocarbons / Q000494:pharmacology; D006801:Humans; D006842:Hydrocarbons, Brominated; D066298:In Vitro Techniques; D008168:Lung; D008171:Lung Diseases / Q000628:therapy*; D010100:Oxygen / Q000493:pharmacokinetics*; D010477:Perfusion; D010920:Placenta / Q000098:blood supply / Q000378:metabolism*
chemical_list: D001802:Blood Substitutes; D003470:Culture Media; D005466:Fluorocarbons; D006842:Hydrocarbons, Brominated; D002245:Carbon Dioxide; C003072:perflubron; D010100:Oxygen