Mitochondrial DNA analysis of Exophiala jeanselmei var. lecanii-corni and Exophiala castellanii.
A Japanese clinical isolate (KU-A-0094) which was identified by de Hoog et al. as Exophiala jeanselmei var. lecanii-corni with difficulty, was compared with 5 strains including the type cultures of E. jeanselmei var. lecanii-corni, var. jeanselmei and E. castellanii using RFLP (restriction fragment length polymorphism) patterns of mtDNA (mitochondrial DNA). RFLP patterns of KUA-0094 were identical with those of E. jeanselmei var. lecanii-corni and different from those of E. castellanii with restriction enzymes of HaeIII, MspI and hindIII. Therefore, de Hoog et al.'s identification of KU-A-0094 was confirmed. Additionally, mtDNA-RFLP patterns of E. jeanselmei var. lecanii-corni and E. jeanselmei var. jeanselmei were also different from each other. Consequently E. jeanselmei var. lecanii-corni seem to be a species in its own right rather than a variant of E. jeanselmei.
Kawasaki
M
M
Department of Dermatology, Kanazawa Medical Univesity, Ishikawa, Japan. masako-k@kanazawa-med.ac.jp
Ishizaki
H
H
Matsumoto
T
T
Matsuda
T
T
Nishimura
K
K
Miyaji
M
M
D004271:DNA, Fungal / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis*; D005093:Exophiala / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D009181:Mycoses / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length
D004271:DNA, Fungal; D004272:DNA, Mitochondrial