Evaluation of evaporative light-scattering detector for combinatorial library quantitation by reversed phase HPLC.
A quantitation study using reversed phase HPLC with UV and evaporative light-scattering detector (ELSD) was conducted on 90 library standards selected from 15 small molecule combinatorial libraries (six standards from each library). This study assessed the quantitation errors using a single calibration curve for rapid purity analysis of combinatorial libraries. The average quantitation error of six standards from one library at 200 microM by UV was 13. 4%, 20.6%, and 60.3%, at 214, 220, and 254 nm, respectively. By ELSD, the average quantitation error of these six standards at 200 micro was only 7.7%. Applying this ELSD calibration curve to 84 standards from 14 structurally diverse libraries, an average quantitation error of 16.4% was obtained. The average quantitation error of all 90 standards from 15 libraries using 15 calibration curves was 18.5%.
Fang
L
L
Axys Pharmaceutical, Inc., 385 Oyster Point Boulevard, Suite 1, South San Francisco, California 94080, USA.
Wan
M
M
Pennacchio
M
M
Pan
J
J
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020650:Combinatorial Chemistry Techniques; D005069:Evaluation Studies as Topic; D008027:Light; D012015:Reference Standards; D012542:Scattering, Radiation