Brain tissue sodium is a ticking clock telling time after arterial occlusion in rat focal cerebral ischemia.
OBJECTIVE
Many patients with acute stroke are excluded from receiving thrombolysis agents within the necessary time limit (3 or 6 hours from stroke onset) because they or their family members are unable provide the time of stroke onset. Brain tissue sodium concentration ([Na(+)]) increases gradually and incessantly during the initial hours of experimental focal cerebral ischemia but only in severely damaged brain regions. We propose that this steady increase in [Na(+)] can be used to estimate the time after arterial occlusion in the rat middle cerebral artery occlusion model of ischemic stroke.


METHODS
Sixteen anesthetized Sprague-Dawley rats underwent permanent middle cerebral artery occlusion combined with bilateral common artery occlusion. After 100 to 450 minutes, diffusion-weighted MRI was used to generate apparent diffusion coefficient (ADC) maps, cerebral blood flow (CBF) was determined with (14)C-iodoantipyrine (in a subset of 7 animals), and the brain was frozen. Autoradiographic CBF sections and punch samples for Na(+) analysis were obtained from the brain at the same level of the MR image. Severely at risk regions were identified with an ADC of <520 microm(2)/s and, in the subset, with both ADC of <520 microm(2)/s and CBF of <40 mL. 100 g(-1). min(-1).


RESULTS
Both CBF and the ADC dropped quickly and remained stable in the initial hours after ischemic onset. Linear regression revealed strong linearity between [Na(+)] and time after onset, with a slope of 0.95 or 1.00 (mEq/kg DW)/min, with both ADC and ADC-plus-CBF criteria, respectively. The 95% CIs at 180 and 360 minutes were between 41 and 52 minutes.


CONCLUSIONS
The time after ischemic onset can be estimated with this 2-step process. First, ADC and CBF are used to identify severely endangered regions. Second, the [Na(+)] in these regions is used to estimate time after onset. The favorable 95% CIs at the time limits for thrombolytic therapy and the availability of measurements of ADC, CBF, and [Na(+)] in humans through the use of MRI suggest that this time-estimation scheme could be used to assess the appropriateness of thrombolysis for patients who do not know when the stroke occurred.
Wang
Y
Y
Department of Anesthesiology, Allegheny General Hospital, Pittsburgh, PA 15212-4772, USA.
Hu
W
W
Perez-Trepichio
A D
AD
Ng
T C
TC
Furlan
A J
AJ
Majors
A W
AW
Jones
S C
SC
D000818:Animals; D001683:Biological Clocks; D015415:Biomarkers; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D017536:Carotid Artery, Common; D016893:Carotid Stenosis / Q000150:complications / Q000378:metabolism*; D002560:Cerebrovascular Circulation; D004058:Diffusion; D004334:Drug Administration Schedule; D007091:Image Processing, Computer-Assisted; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D015912:Thrombolytic Therapy; D013997:Time Factors
D015415:Biomarkers; D012964:Sodium