Antiserum against S-100 protein prevents long term potentiation through a cAMP-related mechanism.
Long term potentiation (LTP) was induced in the CA1 region of rat hippocampal slices by tetanization of the Schaffer collaterals. Local pretreatment of CA1 with serum of rabbits immunized against S-100 prevented the potentiation. However, treatment of the slices with a membrane permeant cAMP analogue, such as 8-Br-cAMP, could protect against the blocking effect of anti S-100 serum. We suggest that in the rat endogenous S-100b is involved in transduction mechanisms during LTP induction, via its ability to stimulate adenylate cyclase. Possible mechanisms of this action are discussed.
Rebaudo
R
R
Department of Neurological Sciences, University of Genoa, Italy.
Melani
R
R
Balestrino
M
M
Cupello
A
A
Haglid
K
K
Hydèn
H
H
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism*; D004558:Electric Stimulation; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000502:physiology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000037:antagonists & inhibitors*
D007106:Immune Sera; D009418:S100 Proteins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP