Monoclonal antibody stains oligodendrocytes and Schwann cells in zebrafish (Danio rerio).
We prepared a monoclonal antibody that recognizes oligodendrocytes and Schwann cells in zebrafish. On immunoblots, the antibody mainly recognized three protein bands of 34 kDa in a membrane fraction from adult zebrafish brain. Medaka fish (Oryzias latipes) also possessed the same protein bands in a membrane fraction. The antibody did not stain neurons, but stained cells in fiber tracts and cranial and spinal nerves. In order to determine the nature of these cells, the staining pattern of the monoclonal antibody was compared with that of a myelin basic protein antiserum. Both antibodies stained oligodendrocytes and Schwann cells in fixed sections from the adult zebrafish. Both antigens were also co-localized in cultured glial cells. Taken together, these results indicate that the new monoclonal antibody recognizes myelinating glial cells in zebrafish and will be useful for the analysis of piscine glia.
Tomizawa
K
K
Department of Biology, Faculty of Science, Okayama University, Japan.
Inoue
Y
Y
Doi
S
S
Nakayasu
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D001921:Brain / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D001931:Brain Mapping; D002478:Cells, Cultured; D004676:Myelin Basic Protein / Q000276:immunology / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D015027:Zebrafish / Q000033:anatomy & histology* / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004676:Myelin Basic Protein