An Entamoeba histolytica rab-like encoding gene and protein: function and cellular location.
We identified here a 576 bp rab-like gene (EhrabB) in Entamoeba histolytica. EhrabB is located 332 bp upstream from the start codon of the Ehcp112 encoding gene, but is transcribed from the complementary strand. The EhrabB open reading frame predicts a 192 amino acid polypeptide (EhRabB) with 40-42% identity to Rab proteins, involved in vesicle docking regulation in endo and exocytic pathways of eukaryotic cells. Transcripts of 0.6 and 0.97 kb were detected by the EhrabB probe in northern blot assays. Using specific antibodies, EhRabB was located in small cytoplasmic vesicles by confocal microscopy. During phagocytosis, EhRabB was initially translocated to the plasma membrane and to the phagocytic mouths. The protein diminished after 10 min phagocytosis, suggesting that EhRabB could be participating in the regulation of the endocytosis process.
Rodríguez
M A
MA
Departamento de Patología Experimental. Centro de Investigación y de Estudios Avanzados del IPN. A.P. 14-740, D.F. 07300, México, Mexico. marodri@enigma.red.cinvestav.mx
García-Pérez
R M
RM
García-Rivera
G
G
López-Reyes
I
I
Mendoza
L
L
Ortiz-Navarrete
V
V
Orozco
E
E
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan; D001692:Biological Transport; D015152:Blotting, Northern; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004748:Entamoeba histolytica / Q000235:genetics* / Q000378:metabolism*; D017125:Genes, Protozoan; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000276:immunology; D014158:Transcription, Genetic; D020691:rab GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*
D000913:Antibodies, Protozoan; D011994:Recombinant Proteins; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D020691:rab GTP-Binding Proteins