title: Detection of Pseudomonas savastanoi pv. savastanoi in olive plants by enrichment and PCR.
abstract: The sequence of the gene iaaL of Pseudomonas savastanoi EW2009 was used to design primers for PCR amplification. The iaaL-derived primers directed the amplification of a 454-bp fragment from genomic DNA isolated from 70 strains of P. savastanoi, whereas genomic DNA from 93 non-P. savastanoi isolates did not yield this amplified product. A previous bacterial enrichment in the semiselective liquid medium PVF-1 improved the PCR sensitivity level, allowing detection of 10 to 100 CFU/ml of plant extract. P. savastanoi was detected by the developed enrichment-PCR method in knots from different varieties of inoculated and naturally infected olive trees. Moreover, P. savastanoi was detected in symptomless stem tissues from naturally infected olive plants. This enrichment-PCR method is more sensitive and less cumbersome than the conventional isolation methods for detection of P. savastanoi.
authors.affiliation: Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Penyalver
authors.forename: A
authors.initials: A
authors.lastname: García
authors.forename: A
authors.initials: A
authors.lastname: Ferrer
authors.forename: E
authors.initials: E
authors.lastname: Bertolini
authors.forename: M M
authors.initials: MM
authors.lastname: López
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D005798:Genes, Bacterial; D007210:Indoleacetic Acids / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D019684:Magnoliopsida / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D007210:Indoleacetic Acids; C030737:indoleacetic acid; D008239:Lysine