Sperm-mediated preparation of transgenic Xenopus laevis and transmission of transgenic DNA to the next generation.
Sperm-mediated transgenesis of frogs Xenopus laevis with retroviral Rous sarcoma virus DNA is described and the transgenic progeny is characterized. Correlation between the high expression of src gene and defective morphogenesis of frog embryos is discussed.
Jonák
J
J
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. jjon@img.cas.cz
D000818:Animals; D030801:Animals, Genetically Modified; D001358:Avian Sarcoma Viruses / Q000235:genetics; D015139:Blotting, Southern; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D018014:Gene Transfer Techniques; D066298:In Vitro Techniques; D008297:Male; D010063:Ovum / Q000166:cytology; D016133:Polymerase Chain Reaction; D013094:Spermatozoa / Q000166:cytology*; D019076:Transgenes; D014982:Xenopus laevis / Q000235:genetics*; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism
D004279:DNA, Viral; D019061:src-Family Kinases