Primary structure, genomic organization, and functional and electrogenic characteristics of human system N 1, a Na+- and H+-coupled glutamine transporter.
We have cloned the human Na(+)- and H(+)-coupled amino acid transport system N (hSN1) from HepG2 liver cells and investigated its functional characteristics. Human SN1 protein consists of 504 amino acids and shows high homology to rat SN1 and rat brain glutamine transporter (GlnT). When expressed in mammalian cells, the transport function of human SN1 could be demonstrated with glutamine as the substrate in the presence of LiCl (instead of NaCl) and cysteine. The transport activity was saturable, pH-sensitive, and specific for glutamine, histidine, asparagine, and alanine. Analysis of Li(+) activation kinetics showed a Li(+):glutamine stoichiometry of 2:1. When expressed in Xenopus laevis oocytes, the transport of glutamine or asparagine via human SN1 was associated with inward currents under voltage-clamped conditions. The transport function, monitored as glutamine- or asparagine-induced currents, was saturable, Na(+)-dependent, Li(+)-tolerant, and pH-sensitive. The transport cycle was associated with the involvement of more than one Na(+) ion. Uptake of asparagine was directly demonstrable in these oocytes by using radiolabeled substrate, and this uptake was inhibited by membrane depolarization. In addition, simultaneous measurement of asparagine influx and charge influx in the same oocyte yielded an asparagine:charge ratio of 1. These data suggest that SN1 mediates the influx of two Na(+) and one amino acid substrate per transport cycle coupled to the efflux of one H(+), rendering the transport process electrogenic.
Fei
Y J
YJ
Departments of Biochemistry and Molecular Biology, and Obstetrics and Gynecology, Medical College of Georgia, Augusta, Georgia 30912, USA.
Sugawara
M
M
Nakanishi
T
T
Huang
W
W
Wang
H
H
Prasad
P D
PD
Leibach
F H
FH
Ganapathy
V
V
D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D001216:Asparagine / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D005091:Exons; D005260:Female; D015723:Gene Library; D005973:Glutamine / Q000378:metabolism*; D006801:Humans; D007438:Introns; D008099:Liver / Q000166:cytology; D008564:Membrane Potentials; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009865:Oocytes; D018408:Patch-Clamp Techniques; D011522:Protons; D051381:Rats; D017422:Sequence Analysis, DNA; D012964:Sodium / Q000378:metabolism*; D013045:Species Specificity; D013379:Substrate Specificity; D014982:Xenopus laevis
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D011522:Protons; C403222:system N protein 1; D005973:Glutamine; D001216:Asparagine; D012964:Sodium