The protein encoded by the proto-oncogene DEK changes the topology of chromatin and reduces the efficiency of DNA replication in a chromatin-specific manner.
The structure of chromatin regulates the genetic activity of the underlying DNA sequence. We report here that the protein encoded by the proto-oncogene DEK, which is involved in acute myelogenous leukemia, induces alterations of the superhelical density of DNA in chromatin. The change in topology is observed with chromatin but not with naked DNA and does not involve dissociation of core histones from chromatin. Moreover, these effects require histone H2A/H2B dimers in addition to histone H3/H4. We additionally tested whether the DEK protein affects DNA-utilizing processes and found that the DEK protein substantially reduces the replication efficiency of chromatin but not of naked DNA templates.
Alexiadis
V
V
University of Konstanz, Department of Biology, 78457 Konstanz, Federal Republic of Germany.
Waldmann
T
T
Andersen
J
J
Mann
M
M
Knippers
R
R
Gruss
C
C
D015153:Blotting, Western; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA / Q000096:biosynthesis*; D004278:DNA, Superhelical; D004279:DNA, Viral / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D009690:Nucleic Acid Conformation; D015513:Oncogene Proteins / Q000502:physiology*; D000075223:Poly-ADP-Ribose Binding Proteins; D000090063:Proto-Oncogene Mas; D011994:Recombinant Proteins / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004278:DNA, Superhelical; D004279:DNA, Viral; C084738:DEK protein, human; D006657:Histones; C000715470:MAS1 protein, human; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D000090063:Proto-Oncogene Mas; D011994:Recombinant Proteins; D004247:DNA