title: Initial experiences of a testicular sperm extraction programme for assisted reproduction in Ireland.
abstract: BACKGROUND
  The technique aspirating spermatozoa from the testis is a new development in male infertility treatment. It is appropriate for infertile couples where the male has azoospermia but is still producing live motile spermatozoa in the testes.
  
  
  OBJECTIVE
  To describe the initial experiences of a testicular sperm extraction programme (TESE) coupled with intracytoplasmic sperm injection (ICSI) in 18 men during 1998.
  
  
  METHODS
  Spermatozoa were obtained by direct aspiration from the testes using a 16 gauge needle with cannula and negative suction under local anaesthetic. All samples obtained were to be cryopreserved for use in a subsequent ICSI cycle.
  
  
  RESULTS
  All five men with congenital bilateral absence of the vas deferens to be carriers of cystic fibrosis gene mutations. No gene deletions were found in their wives. No other cyto or molecular genetic abnormalities were otherwise found. Twenty one procedures were carried out. The mean number of aspirations was 1.72. Eleven samples from 10 men had sperm suitable for ICSI post-freeze. Post-procedure pain was the universal side-effect. Eight couples had a single attempt at ICSI, two couples two each. Fertilisation rate was 71.4%. Two pregnancies were achieved.
  
  
  CONCLUSIONS
  TESE may give hope in selected cases of azoospermia of fathering a child without the involvement of a third party.
authors.affiliation: Human Assisted Reproduction Ireland (HART) Unit, Rotunda Hospital, Dublin.
authors.forename: S
authors.initials: S
authors.lastname: Jacob
authors.forename: H
authors.initials: H
authors.lastname: Spillane
authors.forename: D
authors.initials: D
authors.lastname: Keane
authors.forename: K
authors.initials: K
authors.lastname: Waite
authors.forename: G
authors.initials: G
authors.lastname: McSorley
authors.forename: R F
authors.initials: RF
authors.lastname: Harrison
mesh_terms: D000328:Adult; D015925:Cryopreservation; D005260:Female; D006801:Humans; D007494:Ireland; D008297:Male; D009845:Oligospermia; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D020858:Tissue and Organ Harvesting / Q000379:methods