PCR detection of host and HIV-1 sequences from archival brain tissue.
Mutations in CCR5 and CCR2b have been recently shown to affect disease progression towards AIDS. A role for these host genotypes in AIDS dementia complex (ADC) has also been postulated but remains unclear. Additionally, brain-derived envelope sequences from HIV-1 have been associated with ADC but their specific contribution to pathogenesis remains uncertain. This study demonstrates the successful use of PCR techniques to isolate host CCR5 and CCR2b, and HIV-1 V3 sequences from paraffin embedded tissues from patients with and without ADC. PCR amplification from archival tissue offers a novel approach for studying the interactions between potential neuroprotective elements in the host and virulence determinants in HIV that may contribute to differences in susceptibility to ADC.
Smith
K M
KM
Program in Genetics, Tufts University, Sackler School of Biological and Biomedical Sciences, 136 Harrison Avenue, Boston, Massachusetts, MA 02111, USA.
Crandall
K A
KA
Kneissl
M L
ML
Navia
B A
BA
D015526:AIDS Dementia Complex / Q000378:metabolism / Q000821:virology; D000595:Amino Acid Sequence; D001479:Basal Ganglia / Q000737:chemistry / Q000821:virology; D001921:Brain / Q000821:virology*; D001923:Brain Chemistry; D004247:DNA / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D018450:Disease Progression; D019143:Evolution, Molecular; D005625:Frontal Lobe / Q000737:chemistry / Q000821:virology; D015752:Genes, env; D020022:Genetic Predisposition to Disease; D005838:Genotype; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D010446:Peptide Fragments / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics; D054390:Receptors, CCR2; D019713:Receptors, CCR5 / Q000235:genetics*; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000737:chemistry / Q000821:virology; D014793:Visual Cortex / Q000737:chemistry / Q000821:virology
C515334:CCR2 protein, human; D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D054390:Receptors, CCR2; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D004247:DNA