Active-oxygen involvement in canine NK-mediated cytotoxicity.
We examined the relationship between natural killer (NK) cell-mediated cytotoxicity, the produced active-oxygen and cytotoxic factor (CF) release in co-culturing canine NK cells with tumor cells (CL-1 target cells). In co-culturing, the adding of n-propyl gallate (active-oxide scavenger) removed the produced active-oxygen, which inhibited NK cell-mediated cytotoxicity and the CF release. Moreover, adding of this agent inhibited the tyrosine phosphorylation of NK intracellular protein which observed in co-culturing. Therefore, the active-oxygen produced from canine NK cells are thought to relate the signal transduction in NK-mediated cytotoxicity.
Nariai (Nakada)
N
N
Laboratory Animal Facility, School of Medicine, Yokohama City University, Yokohama, Kanagawa, Japan.
Kitagawa
K
K
Nariai
K
K
Kosaka
T
T
Kuwabara
M
M
Kiuchi
Y
Y
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D018920:Coculture Techniques; D003602:Cytotoxicity, Immunologic; D004285:Dogs / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D010100:Oxygen; D019000:Phosphotyrosine / Q000276:immunology; D011435:Propyl Gallate / Q000008:administration & dosage / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D019000:Phosphotyrosine; D011435:Propyl Gallate; D010100:Oxygen