title: Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.
abstract: An extremely halophilic Archaeon belonging to the order Halobacteriales was isolated from the solar salterns of Cabo Rojo, Puerto Rico. The organism, designated strain PR5T, is rod-shaped, non-motile and requires at least 12% (w/v) NaCl to grow. The strain is highly thermotolerant: its temperature optimum is 50 degrees C and growth is possible up to 60 degrees C. Polar lipid analysis revealed the presence of the bis-sulfated glycolipid S2-DGD-1 as sole glycolipid and the absence of the glycerol diether analogue of phosphatidylglycerosulfate. Both C20,C20 and C20,C25 core lipids are present. The G+C content of the DNA is 63.3 mol%. According to 16S rDNA sequence data, strain PR5T is closely related to the representatives of the genera Haloterrigena and Natrinema, but on the basis of its phenotypic properties, 16S rDNA sequence and DNA-DNA hybridization studies, strain PR5T cannot be assigned to any of the recognized species within these genera. On the basis of its polar lipid composition, the isolate has been assigned to the genus Haloterrigena. The creation of a new species, Haloterrigena thermotolerans, is therefore proposed to accommodate this isolate. The type strain is strain PR5T (= DSM 11552T = ATCC 700275T).
authors.forename: R
authors.initials: R
authors.lastname: Montalvo-Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: López-Garriga
authors.forename: R H
authors.initials: RH
authors.lastname: Vreeland
authors.forename: A
authors.initials: A
authors.lastname: Oren
authors.forename: A
authors.initials: A
authors.lastname: Ventosa
authors.forename: M
authors.initials: M
authors.lastname: Kamekura
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D016931:Halobacteriales / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011647:Puerto Rico; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride