title: Fluorescence line-narrowing detection in chromatography and electrophoresis.
abstract: A review of the basic aspects of fluorescence line-narrowing spectroscopy (FLNS) and its coupling with thin-layer chromatography (TLC) and polyacrylamide gel electrophoresis (PAGE) for off-line high-resolution low temperature spectral characterization is discussed. This is followed by a description of the on-line interfacing of capillary electrophoresis (CE) and capillary electrochromatography (CEC) with FLN detection. CE/ CEC-FLNS instrumentation and its applications for spectral identification of closely related analytes are also presented. Future prospects of micro and capillary high performance liquid chromatography (HPLC) with on-line high-resolution low temperature spectroscopic identification are considered.
authors.affiliation: Department of Chemistry, Iowa State University, Ames 50011, USA. jankowiak@ameslab.gov
authors.forename: R
authors.initials: R
authors.lastname: Jankowiak
authors.forename: K P
authors.initials: KP
authors.lastname: Roberts
authors.forename: G J
authors.initials: GJ
authors.lastname: Small
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods*