Separation of enantiomers by packed capillary electrochromatography on a cellulose-based stationary phase.
Separation of enantiomers was performed by applying packed capillary electrochromatography (CEC). Fused-silica capillaries of different lengths with an inner diameter of 100 microm were packed with a cellulose derivative immobilized onto macroporous silica gel. Parameters such as content of modifier in the mobile phase, concentration and pH of the buffer were varied for a set of test capillaries to determine their influence on enantioselectivity. In packed CEC the highest influence on resolution of the test racemates was found by changing the acetonitrile content, while variation of the buffer concentration mostly affects the electroosmotic velocity. The performance of packed CEC and nano-LC was also compared. Packed CEC showed much better column efficiency and enantioselectivity under similar flow/electroosmotic velocity.
Mayer
S
S
Novartis Pharma AG, Pharmaceutical Research, Core Technology Area, Basel, Switzerland.
Briand
X
X
Francotte
E
E
D002021:Buffers; D002482:Cellulose / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013237:Stereoisomerism
D002021:Buffers; D002482:Cellulose