High-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for the analysis of xenobiotic metabolites in rat urine: application to the metabolites of 4-bromoaniline.
The use of HPLC-ICP-MS for the profiling and quantification of the metabolites of 4-bromoaniline following reversed-phase gradient chromatography is demonstrated. In the 0-8 h post dose sample, which contained the highest concentrations of compound-related material, it was possible to detect at least 16 metabolites of the compound. The methodology described offers the possibility of obtaining metabolite profiles and quantification for drugs and other xenobiotics in biological fluids and excreta without the requirement for radiolabelled tracers.
Nicholson
J K
JK
Imperial College of Science Technology and Medicine, South Kensington, London, UK.
Lindon
J C
JC
Scarfe
G
G
Wilson
I D
ID
Abou-Shakra
F
F
Castro-Perez
J
J
Eaton
A
A
Preece
S
S
D000814:Aniline Compounds / Q000494:pharmacology* / Q000652:urine*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015262:Xenobiotics / Q000493:pharmacokinetics*
D000814:Aniline Compounds; D015262:Xenobiotics; C023621:4-bromoaniline