title: Appearance of hair follicle-inducible mesenchymal cells in the rat embryo.
abstract: Rat vibrissa follicle morphogenesis starts around 13 days of gestation. By day 14 mesenchymal cells have already aggregated as 'condensations' beneath the initial hair bud. Some of the mesenchymal cells will form a dermal papilla, having profound effects on hair follicle formation. The appearance of follicle-inducing mesenchymal cells in the process of vibrissa follicle development was examined. Mesenchymal cells were isolated from the developing site of vibrissa follicles at 13 days or at later stages and amplified in mass culture, harvested and transplanted in association with the epithelium. It was demonstrated that 13-day mesenchymal cells did not induce any hair bulbs but those from 14 days or later stages could induce hair-producing new bulbs or new follicles depending on the association with the follicle epithelium or with the glabrous sole epidermis of the adult rats, respectively. Further, clones having hair bulb-inducing ability were obtained from 14- and 15-day mass-cultured mesenchymal cells. Based on these and other results, it was concluded that mesenchymal cells having follicle-inducing ability are present at least by 14 days in the future whisker pad region. This suggests that the differentiation of the dermal papilla cells must start before the initial hair bud stage.
authors.affiliation: Department of Biology, Faculty of Science, Chiba University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Osada
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D018859:Hair Follicle / Q000166:cytology / Q000196:embryology*; D008297:Male; D008648:Mesoderm / Q000166:cytology*; D051381:Rats; D011916:Rats, Inbred F344; D014180:Transplantation; D014738:Vibrissae / Q000196:embryology*