title: Burkholderia uboniae sp. nov., L-arabinose-assimilating but different from Burkholderia thailandensis and Burkholderia vietnamiensis.
abstract: A polar multitrichous gram-negative motile rod, EY 3383, originally identified as Burkholderia thailandensis, revealed a DNA-DNA reassociation rate of 36.7%, under stringent conditions, with the type strain of B. thailandensis, despite the 16S rDNA homology value between two type strains being as high as 97.9%. The strain was clearly differentiated from the type strain of B. thailandensis by physiological, bio-chemical, and nutritional characteristics, without significant difference in cellular fatty acid and lipid composition. Based on the results of 16S rDNA sequence analysis, DNA-DNA hybridization and phenotypic characterization, Burkholderia uboniae sp. nov. is herein proposed. The type strain is NCTC 13147=EY 3383, isolated on 8 December 1989 from surface soil along the roadside in Ubon Ratchathani, Thailand. Major respiratory quinone is ubiquinone-8(Q8). G+C content of DNA is 69.71%.
authors.affiliation: Department of Microbiology, Gifu University School of Medicine, Gifu, Japan. achei-08@fa2.so-net.ne.jp
authors.forename: E
authors.initials: E
authors.lastname: Yabuuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Ezaki
authors.forename: M
authors.initials: M
authors.lastname: Ikedo
authors.forename: S
authors.initials: S
authors.lastname: Dejsirilert
authors.forename: N
authors.initials: N
authors.lastname: Fujiwara
authors.forename: T
authors.initials: T
authors.lastname: Naka
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001089:Arabinose / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D019117:Burkholderia / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D014451:Ubiquinone / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D001089:Arabinose; C030778:ubiquinone 8