Effect of inhibition of cyclooxygenase on pre- and postjunctional actions of peroxides in the iris-ciliary body.
In the present study, we investigated the effect of inhibition of cyclooxygenase (COX) with flurbiprofen (FBF) on peroxide-induced enhancement of field-stimulated [3H]norepinephrine ([3H]NE) release from bovine isolated irides. Furthermore, the effect of FBF was examined on peroxide-induced attenuation of contractions evoked by carbachol on this tissue. Irides were prepared for studies of neurotransmitter release and for measurement of contractile tension in vitro. Pretreatment of tissues with FBF (10 microM) caused significant (P < 0.001) rightward shifts of concentration-response curves to H2O2 and also decreased cumene hydroperoxide (cuOOH)-induced enhancement of evoked [3H]NE release. FBF (10 microM) partially prevented the attenuation of carbachol-induced contractions induced by H2O2 (300 microM) and cuOOH (300 microM). We conclude that inhibition of the biosynthesis of prostanoids reduced both the prejunctional stimulatory effects of H2O2 and cuOOH on sympathetic neurotransmission and inhibitory effects of peroxides on carbachol-induced contractions the in the bovine isolated iris.
Graham
J
J
Department of Pharmaceutical and Administrative Sciences, School of Pharmacy and Allied Health Professions, Creighton University, Omaha, NE 68178, USA.
Awe
S O
SO
LeDay
A M
AM
Rice
J C
JC
Ramos
G
G
Harris
L C
LC
Opere
C A
CA
Ohia
S E
SE
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002417:Cattle; D018679:Cholinergic Agonists / Q000494:pharmacology; D002924:Ciliary Body / Q000187:drug effects* / Q000201:enzymology / Q000294:innervation; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005260:Female; D005480:Flurbiprofen / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D007498:Iris / Q000187:drug effects* / Q000201:enzymology / Q000294:innervation; D009119:Muscle Contraction / Q000187:drug effects; D009638:Norepinephrine / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D010545:Peroxides / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000187:drug effects / Q000378:metabolism*; D014316:Tritium
D001555:Benzene Derivatives; D018679:Cholinergic Agonists; D016861:Cyclooxygenase Inhibitors; D016877:Oxidants; D010545:Peroxides; D014316:Tritium; D005480:Flurbiprofen; D002217:Carbachol; D006861:Hydrogen Peroxide; D011451:Prostaglandin-Endoperoxide Synthases; C007164:cumene hydroperoxide; D009638:Norepinephrine