A glucose transporter chimera confers a dominant negative glucose starvation phenotype in Saccharomyces cerevisiae.
A family of glucose transporters mediates glucose uptake in Saccharomyces cerevisiae. We show that the dominant mutation GSF4-1, which impairs glucose repression of SUC2, results in a nonfunctional chimera of the transporters Hxt1p and Hxt4p. Hxt1/4p inhibits the function of wild-type glucose transporters. Similar mutations may facilitate analysis of the major facilitator superfamily.
Sherwood
P W
PW
Department of Genetics and Development, Columbia University, New York, NY 10032, USA.
Katic
I
I
Sanz
P
P
Carlson
M
M
D001483:Base Sequence; D002678:Chimera; D004271:DNA, Fungal; D005947:Glucose / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D009004:Monosaccharide Transport Proteins; D005947:Glucose