Pooled human immunoglobulins reduce adhesion of Pseudomonas aeruginosa in a parallel plate flow chamber.
The influence of pooled polyclonal immunoglobulin (IgG) interactions with both bacteria and model substrates in altering Pseudomonas aeruginosa surface adhesion is reported. Opsonization of this pathogen by polyclonal human IgG and preadsorption of IgG to glass surfaces both effectively reduce initial deposition rates and surface growth of P. aeruginosa IFO3455 from dilute nutrient broth in a parallel plate flow chamber. Polyclonal IgG depleted of P. aeruginosa-specific antibodies reduces the initial deposition rate or surface growth to levels intermediate between exposed and nonexposed IgG conditions. Bacterial surface properties are changed in the presence of opsonizing IgG. Plateau contact angle analysis via sessile drop technique shows a drop in P. aeruginosa surface hydrophobicity after IgG exposure consistent with a more hydrophilic IgG surface coat. Zeta potential values for opsonized versus nonopsonized bacteria exhibit little change. X-ray photoelectron spectroscopy measurements provide surface compositional evidence for IgG attachment to bacterial surfaces. Surface elemental ratios attributed to IgG protein signals versus those attributed primarily to bacterial polysaccharide surface or lipid membrane change with IgG opsonization. Direct evidence for antibody-modified P. aeruginosa surface properties correlates both with reduction of bacterial adhesion to glass surfaces under flow in nutrient medium reported and previous reports of IgG efficacy against P. aeruginosa motility in vitro and infection in vivo.
Poelstra
K A
KA
Anthony G. Gristina Institute for Biomedical Research, Herndon, Virginia 20170, USA.
van der Mei
H C
HC
Gottenbos
B
B
Grainger
D W
DW
van Horn
J R
JR
Busscher
H J
HJ
D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D001431:Bacteriological Techniques; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology*; D007075:Immunoglobulin M / Q000494:pharmacology*; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000254:growth & development / Q000502:physiology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M