title: Molecular and phylogenetic analysis of Cryptosporidium muris from various hosts.
abstract: Isolates of Cryptosporidium muris and C. serpentis were characterized from different hosts using nucleotide sequence analysis of the rDNA 18S and ITS1 regions, and the heat-shock (HSP-70) gene. Phylogenetic analysis confirmed preliminary evidence that C. muris is not a uniform species. Two distinct genotypes were identified within C. muris; (1) C. muris genotype A; comprising bovine and camel isolates of C. muris from different geographical locations, and (2) C. muris genotype B comprising C. muris isolates from mice, a hamster, a rock hyrax and a camel from the same enclosure. These 2 genotypes may represent separate species but further biological and molecular studies are required for confirmation.
authors.affiliation: World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, Murdoch, WA, Australia. morgan@numbat.murdoch.au
authors.forename: U M
authors.initials: UM
authors.lastname: Morgan
authors.forename: L
authors.initials: L
authors.lastname: Xiao
authors.forename: P
authors.initials: P
authors.lastname: Monis
authors.forename: I
authors.initials: I
authors.lastname: Sulaiman
authors.forename: I
authors.initials: I
authors.lastname: Pavlasek
authors.forename: B
authors.initials: B
authors.lastname: Blagburn
authors.forename: M
authors.initials: M
authors.lastname: Olson
authors.forename: S J
authors.initials: SJ
authors.lastname: Upton
authors.forename: N V
authors.initials: NV
authors.lastname: Khramtsov
authors.forename: A
authors.initials: A
authors.lastname: Lal
authors.forename: A
authors.initials: A
authors.lastname: Elliot
authors.forename: R C
authors.initials: RC
authors.lastname: Thompson
mesh_terms: D000818:Animals; D002162:Camelus; D002417:Cattle; D006224:Cricetinae; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D017125:Genes, Protozoan; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D051379:Mice; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012911:Snakes
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D018840:HSP70 Heat-Shock Proteins; D012337:RNA, Ribosomal, 18S