Crystallization and preliminary X-ray analysis of human grancalcin, a novel cytosolic Ca2+-binding protein present in leukocytes.
Recombinant human grancalcin, a calcium-binding protein from leukocytes, has been crystallized in the presence or absence of Ca(2+) by the vapor-diffusion method. Two crystal forms of apo grancalcin were obtained: space group P2(1), with unit-cell parameters a = 48.4, b = 81.1, c = 46.6 A, beta = 111.3 degrees, diffracting to 1.9 A, and space group C2, with unit-cell parameters a = 97.0, b = 51.9, c = 75.9 A, beta = 108.5 degrees, diffracting to 2.4 A. Crystals were also grown in the presence of 5 mM Ca(2+). They also belong to space group C2, with unit-cell parameters a = 97.4, b = 50.3, c = 77.6 A, beta = 108.2 degrees, which are very similar to the second apo grancalcin form. These crystals diffract to 2.5 A.
Han
Q
Q
Biotechnology Research Institute, NRC, Montreal, Quebec, Canada.
Jia
J
J
Li
Y
Y
Lollike
K
K
Cygler
M
M
D001059:Apoproteins / Q000302:isolation & purification; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000378:metabolism; D003625:Data Collection; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D011485:Protein Binding
D001059:Apoproteins; D002135:Calcium-Binding Proteins; C503121:GCA protein, human; D002118:Calcium