title: Chloroperoxidase-mediated chlorination of aromatic groups in fulvic acid.
abstract: The aim of the present study is to investigate whether exo-enzymatically mediated chlorination of fulvic acid (FA) results in the formation of chlorinated groups within the macromolecules which correspond to those which were previously detected in soil and surface water samples. The chlorination was carried out by exposing FA to a commercial chloroperoxidase (CPO) in the presence of chloride and hydrogen peroxide. The exposed FA was then chemically degraded using an oxidative technique and finally analysed for four different aromatic groups and their chlorinated analogues. The particular aromatic groups included were the methyl esters of 4-ethoxybenzoic acid, 3-methoxy-4-ethoxybenzoic acid, 3,4-diethoxybenzoic acid, and 3,5-dimethoxy-4-ethoxybenzoic acid, along with their mono- and dichlorinated analogues. Prior to the chemical degradation procedure, the FA was analysed for AOX (adsorbable organic halogens) and chlorinated acetic acids. The original FA contained 1.4 mg Cl(org) g(-1) and detectable amounts of two chlorinated aromatic groups. After exposure to the enzyme, the concentration of AOX increased to 44.3 mg Cl(org) g(-1) and detectable amounts of four chlorinated aromatic groups as well as di- and trichloroacetic acid were found.
authors.affiliation: Institute of Environmental Geochemistry, University of Heidelberg, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Niedan
authors.forename: I
authors.initials: I
authors.lastname: Pavasars
authors.forename: G
authors.initials: G
authors.lastname: Oberg
mesh_terms: D000085:Acetates / Q000737:chemistry; D001578:Benzopyrans / Q000737:chemistry* / Q000378:metabolism*; D002711:Chloride Peroxidase / Q000737:chemistry / Q000378:metabolism*; D017606:Chlorine Compounds / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry
chemical_list: D000085:Acetates; D001578:Benzopyrans; D017606:Chlorine Compounds; D006861:Hydrogen Peroxide; D002711:Chloride Peroxidase; C005023:fulvic acid