Plasmacytoma of the tonsil with AL amyloidosis: evidence of post-fibrillogenic proteolysis of the fibril protein.
We report of a 58-year-old Caucasian man who was referred to the University Hospital with a greatly enlarged left tonsil which showed calcifications on computed-tomography scans. Histopathology revealed a plasmacytoma with secondary AL amyloidosis, ossifications, and multinucleated foreign-body-type giant cells. N-terminal sequencing of amyloid-fibril proteins purified from the formalin-fixed tissue showed the presence of two proteins of different size; these were of lambda-light-chain origin (subgroup V), measured approximately 15.2 kDa and 10.5 kDa, and had identical N-terminal ends (YVLTQPP). When the amyloid deposits were immunolabeled with a polyclonal antibody directed against lambda light chain, they showed two staining patterns: some deposits showed intense immunolabeling while others were not immunoreactive. Immunostaining of amyloid was completely absent after protease pre-treatment. Immunoelectron microscopy with gold-labeled secondary antibodies showed staining that was spatially related to amyloid fibrils and suggested that the antibody probably detected the fibril protein. Therefore, our hypothesis in this case is that the different immunostaining patterns are due to a post-fibrillogenic proteolysis of the fibril protein at the C-terminal end of the light chain, as indicated by the presence of two differently sized lambda-light-chain fragments with identical N-terminal ends.
Röcken
C
C
Institute of Pathology, Otto-von-Guericke-University, Magdeburg, Germany. christoph.roecken@medizin.uni-magdeburg.de
Hegenbarth
V
V
Schmitz
M
M
Stix
B
B
Schade
G
G
Mohnert
A
A
Roessner
A
A
D000686:Amyloidosis / Q000150:complications*; D002114:Calcinosis / Q000150:complications / Q000000981:diagnostic imaging; D006801:Humans; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000150:complications* / Q000473:pathology; D014066:Palatine Tonsil / Q000000981:diagnostic imaging / Q000473:pathology; D010447:Peptide Hydrolases / Q000378:metabolism; D010954:Plasmacytoma / Q000150:complications* / Q000473:pathology; D000685:Serum Amyloid A Protein / Q000378:metabolism; D014057:Tomography, X-Ray Computed
D000685:Serum Amyloid A Protein; D010447:Peptide Hydrolases