title: C. elegans KLP-11/OSM-3/KAP-1: orthologs of the sea urchin kinesin-II, and mouse KIF3A/KIFB/KAP3 kinesin complexes.
abstract: Kinesins are intracellular multimeric transport motor proteins that move cellular cargo on microtubule tracks. It has been shown that the sea urchin KRP85/95 holoenzyme associates with a KAP115 non-motor protein, forming a heterotrimeric complex in vitro, called the Kinesin-II. Here we describe isolation of a cDNA clone corresponding to the klp-11 kinesin in C. elegans. Our sequence analysis of the encoded KLP-11 shows that it shares high homology with the OSM-3 kinesin. We also describe a nematode cDNA encoding KAP-1 that shares extensive homology with the sea urchin KAP115 kinesin associated protein. Sequence-based structural analysis of the OSM-3, KLP-11, and KAP-1, presented here suggests that these may form a heterotrimeric complex. We also describe the presence of a Drosophila armadillo consensus motif in CeKAP-1, first found in spKAP115, that suggests a possible role for the KAP-1 in signal transduction.
authors.affiliation: Department of Ecological Engineering, Toyohashi University of Technology, Japan.
authors.forename: M L
authors.initials: ML
authors.lastname: Khan
authors.forename: M Y
authors.initials: MY
authors.lastname: Ali
authors.forename: Z K
authors.initials: ZK
authors.lastname: Siddiqui
authors.forename: M A
authors.initials: MA
authors.lastname: Shakir
authors.forename: H
authors.initials: H
authors.lastname: Ohnishi
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: S S
authors.initials: SS
authors.lastname: Siddiqui
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism; D016547:Kinesins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics; D012617:Sea Urchins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D029742:Caenorhabditis elegans Proteins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D015801:Helminth Proteins; C484407:KIFAP3 protein, human; C490202:Kif3a protein, mouse; D009124:Muscle Proteins; C093002:OSM-3 protein, C elegans; C074152:kinesin-II; C100815:Kifap3 protein, mouse; D016547:Kinesins