title: The promoter for constitutive expression of the human ICln gene CLNS1A.
abstract: The ICln protein is expressed ubiquitously in mammals. Experiments designed to knock down the ICln protein in NIH 3T3 fibroblasts as well as in epithelial cells led to the conclusion that this protein is crucially involved in volume regulation after cytoplasmic swelling. Reconstitution of the ICln protein in lipid bilayers revealed the ion channel nature of ICln. Here we describe a new human promoter sequence, composed of 89 nucleotides, which is responsible for a highly constitutive expression of the ICln protein. The promoter sequence lacks a TATA box, and the transcription can be effected at multiple sites. In addition to the starting sites, upstream sequence elements are mandatory for an efficient transcription of the ICln gene (CLNS1A). These new nucleotide elements were defined by site-directed mutagenesis.
authors.affiliation: Department of Physiology, University of Innsbruck, Fritz-Pregl Strasse 3, A-6020 Innsbruck, Austria.
authors.forename: E
authors.initials: E
authors.lastname: Scandella
authors.forename: U O
authors.initials: UO
authors.lastname: Nagl
authors.forename: B
authors.initials: B
authors.lastname: Oehl
authors.forename: F
authors.initials: F
authors.lastname: Bergmann
authors.forename: M
authors.initials: M
authors.lastname: Gschwentner
authors.forename: J
authors.initials: J
authors.lastname: Fürst
authors.forename: A
authors.initials: A
authors.lastname: Schmarda
authors.forename: M
authors.initials: M
authors.lastname: Ritter
authors.forename: S
authors.initials: S
authors.lastname: Waldegger
authors.forename: F
authors.initials: F
authors.lastname: Lang
authors.forename: P
authors.initials: P
authors.lastname: Deetjen
authors.forename: M
authors.initials: M
authors.lastname: Paulmichl
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D048429:Cell Size / Q000235:genetics; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D000882:Haplorhini; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D016415:Sequence Alignment; D014162:Transfection
chemical_list: C410035:CLNS1A protein, human; D007473:Ion Channels; D011506:Proteins