Detection of West Nile virus sequences in cerebrospinal fluid.
We have established a sensitive and specific real-time PCR method for detection of West Nile virus. Analysis of specimens obtained during the 1999 New York outbreak indicated the presence of viral sequences In cerebrospinal fluid of all of four Individuals with fatal outcomes, and in only one of four who survived.
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D000293:Adolescent; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014901:West Nile Fever / Q000134:cerebrospinal fluid*; D014902:West Nile virus / Q000235:genetics* / Q000302:isolation & purification