title: Abalone collagens: immunological properties and seasonal changes of their mRNA levels.
abstract: The antisera were raised against pepsin-solubilized abalone collagen and its corresponding gelatin. The reactivity against abalone collagen was higher with the anti-collagen than anti-gelatin antiserum. The two antisera recognized all type I collagens from various vertebrates, whereas these had no reactivity against vertebrate type III and type V collagens. Furthermore, both antisera reacted with only alpha 2(I) chains from chicken, rat, and calf. The strong reactivity was observed against the two antisera in the case of invertebrate and protochordate collagens, especially for turban shell collagen. The seasonal changes of collagen mRNA levels were examined in relation to those of collagen content. Haliotis discus collagens (Hdcols) 1 alpha and 2 alpha coding for abalone collagen pro alpha-chains showed quite similar patterns. The highest mRNA levels in adductor and foot muscles for the two collagens were observed in December and January, in good agreement with the increase of collagen content. The mRNA levels decreased in July and August when collagen content decreased. These results indicate that collagen transcription levels are closely related to collagen contents.
authors.affiliation: Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Yoneda
authors.forename: M N
authors.initials: MN
authors.lastname: Ahsan
authors.forename: M
authors.initials: M
authors.lastname: Nakaya
authors.forename: Y
authors.initials: Y
authors.lastname: Matsubara
authors.forename: T
authors.initials: T
authors.lastname: Ebihara
authors.forename: S
authors.initials: S
authors.lastname: Irie
authors.forename: Y
authors.initials: Y
authors.lastname: Uno
authors.forename: K
authors.initials: K
authors.lastname: Hatae
authors.forename: S
authors.initials: S
authors.lastname: Watabe
mesh_terms: D000818:Animals; D002417:Cattle; D003094:Collagen / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D005260:Female; D008974:Mollusca / Q000378:metabolism*; D012333:RNA, Messenger; D011817:Rabbits; D051381:Rats; D012621:Seasons
chemical_list: D012333:RNA, Messenger; D003094:Collagen