Oxalate-degrading Enterococcus faecalis.
An oxalate-degrading Enterococcus faecalis was isolated from human stools under anaerobic conditions. The bacteria required a poor nutritional environment and repeated subculturing to maintain their oxalate-degrading ability. The E. faecalis produced 3 proteins (65, 48, and 40 kDa) that were not produced by non-oxalate-degrading E. faecalis as examined by SDS-PAGE. Antibodies against oxalyl-coenzyme A decarboxylase (65 kDa) and formyl-coenzyme A transferase (48 kDa) obtained from Oxalobacter formigenes (an oxalate-degrading anaerobic bacterium in the human intestine) reacted with 2 of the proteins (65 and 48 kDa) from the E. faecalis as examined by Western blottings. This is the first report on the isolation of oxalate-degrading facultative anaerobic bacteria from humans.
Hokama
S
S
Department of Urology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa, Japan.
Honma
Y
Y
Toma
C
C
Ogawa
Y
Y
D000328:Adult; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D013293:Enterococcus faecalis / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D010070:Oxalates / Q000378:metabolism*; D011817:Rabbits
D001426:Bacterial Proteins; D003470:Culture Media; D010070:Oxalates