Analysis of classical swine fever virus replication kinetics allows differentiation of highly virulent from avirulent strains.
To study the replication of classical swine fever virus (CSFV) in cell culture, kinetics of viral plus-strand RNA synthesis, of viral structural and non-structural protein expression as well as of secreted and cell-associated infectious virus were determined. Highly virulent, moderately virulent and avirulent strains that were tested in standardized animal experiments to confirm their virulence were used to search for in vitro parameters allowing the differentiation of strains according to their virulence. No significant qualitative or quantitative differences were found between the strains studied when either RNA replication or protein synthesis were investigated. However, the ratio of cell-associated virus versus secreted virus proved to be considerably lower for the highly virulent strains when compared to avirulent or moderately virulent strains. These data suggest that highly virulent strains of CSFV can be distinguished in cell culture from strains with reduced virulence.
Mittelholzer1
C
C
Institute of Virology and Immunoprophylaxis, CH-3147, Mittelhäusern, Switzerland.
Moser2
C
C
Tratschin
J D
JD
Hofmann
M A
MA
D000818:Animals; D015152:Blotting, Northern / Q000662:veterinary; D015153:Blotting, Western / Q000662:veterinary; D001831:Body Temperature; D002460:Cell Line; D006691:Classical Swine Fever / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000472:pathogenicity / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007700:Kinetics; D012367:RNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D014764:Viral Proteins / Q000096:biosynthesis; D014774:Virulence; D014779:Virus Replication / Q000502:physiology*
D012367:RNA, Viral; D014764:Viral Proteins