title: Liquid chromatography tandem mass spectrometric quantitation of sulfamethazine and its metabolites: direct analysis of swine urine by triple quadrupole and by ion trap mass spectrometry.
abstract: This work describes a new method for the quantitation of trace amounts of sulfamethazine (SMZ) and its main metabolite, N4-acetylsulfamethazine (Ac-SMZ), in swine urine, using high-performance liquid chromatography (HPLC) tandem mass spectrometric analysis of crude urine after addition of internal standard and simple dilution with water. The aim was to determine whether residues of this sulfamidic drug, normally administered to swine in order to prevent infectious diseases, were present in urine at levels lower than those permitted by regulatory authorities before human consumption (EU Project SMT, contract number CT 96-2092). A 10 microL volume of diluted urine was injected into a very short, narrow-bore chromatographic column (Zorbax SB-C18 2.1 i. d. x30 mm length, 3.5 microm pore size). Elution of the analytes of interest was achieved in less than seven minutes using a rapid gradient (from 20 to 80% methanol in 3 minutes). Either a PE Sciex API 365 triple quadrupole (QqQ), operated in the selected reaction monitoring (SRM) mode, or a Finnigan LCQ ion trap (IT) mass spectrometer, operated in narrow-range product ion scan, was used as the final detector. Electrospray (ESI) was used as the ionization technique. A comparison of the two tandem mass spectrometers was performed by analyzing the same set of test samples, at three concentration levels, on three different days. Linearity of responses of the calibration standards, intra- and inter-assay precision of the samples, specificity and limits of detection were evaluated for both systems. Both the QqQ and the IT instrument was suitable for rapid, sensitive and specific determination of the analytes, although the overall performance of the QqQ was slightly superior in terms of linearity, precision and sensitivity.
authors.affiliation: Centro Interdipartimentale di servizi di Spettrometria di Massa, University of Firenze, viale G. Pieraccini 6, I-50134 Firenze, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bartolucci
authors.forename: G
authors.initials: G
authors.lastname: Pieraccini
authors.forename: F
authors.initials: F
authors.lastname: Villanelli
authors.forename: G
authors.initials: G
authors.lastname: Moneti
authors.forename: A
authors.initials: A
authors.lastname: Triolo
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013418:Sulfamethazine / Q000652:urine*; D013552:Swine
chemical_list: D000890:Anti-Infective Agents; D013418:Sulfamethazine