Oxidative stress and defence mechanisms of the freshwater cladoceran Daphnia longispina exposed to UV radiation.
The aim of the present study is to evaluate the occurrence of oxidative stress in the cladoceran Daphnia longispina exposed to UV-A and UV-B radiation. The activity of antioxidant enzymes and lipid peroxidation markers is investigated and the protective action of ascorbic acid determined. Results show differences in the lethality radioinduced by UV-A and UV-B. Both UV-A and UV-B exposure cause an important increase in malonaldehyde (MDA) concentration and catalase activity. Ascorbic acid addition reduces the MDA concentration, indicating that the oxidative stress caused by either UV-A or UV-B radiation can be controlled by antioxidants. The increase of the antioxidant enzymes may be a response mechanism to oxidative stress.
Vega
M P
MP
Comisión Nacional de Energía Atómica, Dpto. Radiobiología, Buenos Aires, Argentina. pamavega@cnea.gov.ar
Pizarro
R A
RA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D003470:Culture Media; D003621:Daphnia / Q000187:drug effects / Q000528:radiation effects*; D005618:Fresh Water; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D014466:Ultraviolet Rays
D000975:Antioxidants; D003470:Culture Media; D001205:Ascorbic Acid