Circular dichroism as a detection method in the screening of enantioselective catalysts.
The combination of liquid chromatography (HPLC), UV/Vis-spectroscopy and circular dichroism (CD) can be used to construct a high-throughput screening system to determine the enantioselectivity of enzyme- or metal-catalyzed reduction of acetophenone with formation of (S)- and (R)-1-phenylethanol. Prerequisite for the viability of this system is the experimental finding that the anisotropy factor g is linearly related to the enantiomeric excess (ee) and that it is independent of concentration, thereby excluding possible aggregation effects.
Reetz
M T
MT
Max-Planck-Institut fur Kohlenforschung, Mulheim/Ruhr, Germany. reetz@mpi-muelheim.mpg.de
Kuhling
K M
KM
Hinrichs
H
H
Deege
A
A
D000097:Acetonitriles / Q000494:pharmacology; D000098:Acetophenones / Q000737:chemistry; D016880:Anisotropy; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D002942:Circular Dichroism / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D008956:Models, Chemical; D010626:Phenylethyl Alcohol / Q000737:chemistry; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D000097:Acetonitriles; D000098:Acetophenones; D010626:Phenylethyl Alcohol; C038699:acetophenone; C032159:acetonitrile