title: The salt-inducible kinase, SIK, is induced by depolarization in brain.
abstract: Membrane depolarization of neurons is thought to lead to changes in gene expression that modulate neuronal plasticity. We used representational difference analysis to identify a group of cDNAs that are induced by membrane depolarization or by forskolin, but not by neurotrophins or growth factors, in PC12 pheochromocytoma cells. One of these genes, SIK (salt-inducible kinase), is a member of the sucrose-nonfermenting 1 protein kinase/AMP-activated protein kinase protein kinase family that was also recently identified from the adrenal gland of rats treated with high-salt diets. SIK mRNA is induced up to eightfold in specific regions of the hippocampus and cortex in rats, following systemic kainic acid administration and seizure induction.
authors.affiliation: Department of Pediatrics, University of Southern California, Los Angeles, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Feldman
authors.forename: L
authors.initials: L
authors.lastname: Vician
authors.forename: M
authors.initials: M
authors.lastname: Crispino
authors.forename: W
authors.initials: W
authors.lastname: Hoe
authors.forename: M
authors.initials: M
authors.lastname: Baudry
authors.forename: H R
authors.initials: HR
authors.lastname: Herschman
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D005576:Colforsin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004827:Epilepsy / Q000139:chemically induced / Q000503:physiopathology; D018690:Excitatory Amino Acid Agonists; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D017781:Genes, Immediate-Early / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology; D007608:Kainic Acid; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D009474:Neurons / Q000201:enzymology*; D016716:PC12 Cells; D010802:Phylogeny; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012492:Salts / Q000378:metabolism; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000378:metabolism; D019061:src-Family Kinases / Q000235:genetics*
chemical_list: D018690:Excitatory Amino Acid Agonists; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D012492:Salts; D005576:Colforsin; D012694:Serine; D003513:Cycloheximide; D011505:Protein-Tyrosine Kinases; C000598269:Ptk6 protein, mouse; D019061:src-Family Kinases; D007608:Kainic Acid