Heterogeneity in growth and differentiation characteristics in male and female satellite cells isolated from turkey lines with different growth rates.
The effects of growth- and gender-related differences on satellite cell proliferation and differentiation were investigated using satellite cells isolated from the pectoralis major muscle of a turkey line selected for increased 16-week body weight (F-line) and its unselected randombred control (RBC2-line). Proliferation rates within the F- and RBC2-lines did not differ between sexes. The F-line male and female satellite cells when compared to the RBC2-line male and female satellite cells proliferated at a faster rate. Differentiation rates were increased for the F-line male cells compared to both the F-line female and RBC2-line male satellite cells. No difference in differentiation rate was noted within the RBC2-line satellite cells. For satellite cells from females, the RBC2-line differentiated faster than the F-line. Morphological data on myotube length and the number of nuclei per myotube supported the differentiation data in that F-line male satellite cells had the longest myotubes with the most nuclei, there was no significant difference between myotubes within the RBC2-line, and female-derived myotubes from the RBC2-line were longer than those of the F-line by 96 h of fusion. These data are suggestive of both growth- and gender- related differences in satellite cell proliferation and differentiation.
Velleman
S G
SG
Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691, USA. velleman.1@osu.edu
Liu
X
X
Nestor
K E
KE
McFarland
D C
DC
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D005260:Female; D008297:Male; D010369:Pectoralis Muscles / Q000166:cytology*; D014422:Turkeys / Q000254:growth & development*