Protein synthesis in Escherichia coli at 4 degrees C.
Two-dimensional electrophoresis technology was used to investigate protein synthesis by the mesophilic bacterium Escherichia coli at low temperature. It was confirmed that protein synthesis in E. coli decreased strongly after a temperature downshift from 37 to 4 degrees C. After incubation for 150 min at 4 degrees C, however, the number of synthesized proteins represented 60% of the overall polypeptide number observed at 37 degrees C. Furthermore, the analysis of autoradiograms revealed the overexpression of 69 proteins by shocked bacteria, showing that the number of cold-induced proteins has been significantly underestimated so far.
Perrot
F
F
Faculté des Sciences de Rouen, Mont-Saint-Aignan, France.
Hébraud
M
M
Junter
G A
GA
Jouenne
T
T
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism*
D001426:Bacterial Proteins