[A quantitative method for determining lectin activity on microplates].
A modification of a known quantitative solidphase microtiter plate lectin assay elaborated by Hatakeyama and coauthors developed with biotinylated lectins and ExtrAvidin--alkaline phosphatase conjugate was proposed. The modification was performed in order to use a broad spectra of lectin ligands, including glycopeptides, peptides, etc. The optimal concentrations of the reagents and time of the reagents incubation were selected. Being known from the literature Kdiss for Concanavalin A and monosaccharides: methyl-Man, Man, Glc and Gal were determined in a model experiments. The results were in agreement with the range of monosaccharides specificity for the Concanavalin A. The modification described is relatively simple and sensitive.
Miroshnichenko
O S
OS
A.V. Palladin Institute of Biochemistry of National Academy of Sciences, Ukraine.
D000469:Alkaline Phosphatase / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D007202:Indicators and Reagents; D037102:Lectins / Q000378:metabolism*; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D037102:Lectins; D001710:Biotin; D000469:Alkaline Phosphatase