On-line reversed-phase liquid chromatography hydride generation emission spectrometry: speciation of arsenic in urine of patients intravenously treated with As2O3.
Hydride generation inductively coupled plasma-atomic emission spectrometry (HG ICP-AES) was used as a continuous detection system for the determination of arsenic in the eluate from a high-performance liquid chromatographic (HPLC) system. Four arsenic species [arsenite As(III), arsenate As(V), monomethylarsonate (MMA), and dimethylarsinate (DMA)] present in the urine samples of patients treated intravenously with arsenite, were analyzed separately by HPLC-HG-ICP-AES using a non-polar C18 column. This analytical method allowed the sensitive determination of the arsenic species in the submicrogram per liter range. Urine samples collected on different days after arsenite administration were found to contain arsenite predominantly--monomethylarsonate and dimethylarsinate were also detected.
Do
B
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Laboratoire Central d'Analyses, Pharmacie Centrale des Hôpitaux de Paris, France.
Alet
P
P
Pradeau
D
D
Poupon
J
J
Guilley-Gaillot
M
M
Guyon
F
F
D001151:Arsenic / Q000652:urine*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D010087:Oxides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D012996:Solutions; D013054:Spectrophotometry, Atomic
D001152:Arsenicals; D007202:Indicators and Reagents; D010087:Oxides; D012996:Solutions; D003545:Cysteine; D001151:Arsenic; D000077237:Arsenic Trioxide