A free cysteine residue at the dimer interface decreases conformational stability of Xenopus laevis copper,zinc superoxide dismutase.
The two Cu,Zn superoxide dismutases from the amphibian Xenopus laevis (denoted XSODA and XSODB) display different heat sensitivities, XSODA being more thermolabile than XSODB. In this study, we have investigated the contribution of a free cysteine residue located close to the subunit interface of XSODA to its lower thermal stability. We have found that mutation of residue Cys 150 to Ala in XSODA makes the thermal stability of this enzyme comparable to that of the wild-type XSODB isoenzyme, while the introduction of a cysteine residue in the same position of XSODB renders this enzyme variant much more heat-sensitive. Differential scanning calorimetry experiments showed that XSODA has a melting temperature about 8.5 degrees C lower than that of XSODB. On the contrary, the melting temperature of XSODACys150Ala is very close to that of XSODB, while the melting temperature of XSODBSer150Cys is even lower than that of wild-type XSODA. These data indicate that the free cysteine residue present in XSODA affects not only the reversibility of unfolding of the enzyme but also its conformational stability. We suggest that the large effect of the Cys 150 residue on XSODA stability might be due to incorrect disulfide bond formation or disulfide bond interchange during heat-induced unfolding rather than to alteration of the interaction between the enzyme subunits.
Bonaccorsi di Patti
M C
MC
Department of Biochemical Sciences A. Rossi Fanelli and CNR Center of Molecular Biology, University of Rome La Sapienza, Italy.
Carrì
M T
MT
Gabbianelli
R
R
Da Gai
R
R
Volpe
C
C
Giartosio
A
A
Rotilio
G
G
Battistoni
A
A
D000818:Animals; D002152:Calorimetry, Differential Scanning; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D019281:Dimerization; D004578:Electron Spin Resonance Spectroscopy; D004795:Enzyme Stability; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D014982:Xenopus laevis
D013482:Superoxide Dismutase; D003545:Cysteine