Simultaneous monitoring of vascular contractility, intracellular pH and intracellular calcium in isolated rat mesenteric arteries; effects of weak bases.
We report the first simultaneous measurements of pHi, [Ca2+]i and tension, upon alteration of pHi, in isolated rat mesenteric arteries loaded with both carboxy SNARF and indo-1. In these vessels (pre-contracted by 30 mM KCl) alterations of pHi, by addition and subsequent washout of weak bases, produced complicated effects on tone. Although the changes in contractility did not mirror the changes in pHi they were, at all times, accompanied by parallel changes in [Ca2+]i.
Otter
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Department of Medicine, Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, UK.
Austin
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D000468:Alkalies; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001578:Benzopyrans; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007211:Indoles; D008297:Male; D008638:Mesenteric Arteries / Q000737:chemistry / Q000502:physiology*; D008744:Methylamines / Q000494:pharmacology; D009119:Muscle Contraction / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000502:physiology*; D009284:Naphthols; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines
D000468:Alkalies; D001578:Benzopyrans; D005456:Fluorescent Dyes; D007211:Indoles; D008744:Methylamines; D009284:Naphthols; D012235:Rhodamines; C068301:carboxy-seminaphthorhodaminefluoride; D000643:Ammonium Chloride; D011189:Potassium Chloride; C023336:trimethylamine; C048960:indo-1; D002118:Calcium