Expression of intestine-specific antigen reveals novel pathways of CD8 T cell tolerance induction.
Reactivity to intestinal epithelium-specific antigen was studied by transgenic expression of cytosolic ovalbumin controlled by an enterocyte-specific promoter. Transferred OVA-specific CD8 cells (OT-I) preferentially expanded in mucosal lymphoid tissues and the epithelium but failed to cause tissue damage. In contrast, concomitant VSV-ova infection induced OT-I-mediated epithelial cell destruction that correlated with antigen density. OT-I cells retained in the epithelium exhibited high levels of lytic activity but were unable to produce cytokines. The mice were systemically tolerant to OVA since endogenous CD8 cells were nonresponsive to VSV-ova infection. Thus, intestinal antigen gained access to peripheral tissues via absorption from effete epithelial cells. This system demonstrated a requirement for inflammation to drive pathogenic autoreactivity against enterocytes and identified pathways of intestine-specific immunoregulation.
Vezys
V
V
Department of Medicine, University of Connecticut Health Center, Farmington 06030, USA.
Olson
S
S
Lefrançois
L
L
D000818:Animals; D016827:CD8 Antigens; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018014:Gene Transfer Techniques; D007108:Immune Tolerance; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009928:Organ Specificity; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology*
D016827:CD8 Antigens; D010047:Ovalbumin