title: Association of gastric epithelial apoptosis with the ability of Helicobacter pylori to induce a neutrophil oxidative burst.
abstract: Both polymorphonuclear cell infiltration and increased epithelial apoptosis are seen in gastric mucosa in the presence of Helicobacter pylori infection. This study examined the association between bacterial ability to stimulate an oxidative burst in neutrophils and epithelial apoptosis. Biopsy specimens were obtained from 15 patients to detect apoptotic cells by the TUNEL method. H. pylori strains isolated from corresponding stomach biopsy samples were tested for the ability to stimulate an oxidative burst in human neutrophils. Neutrophils were isolated from healthy subjects without H. pylori infection and the oxidative burst was measured by flow cytometry with dichlorofluorescein diacetate. Stimulation with H. pylori increased both the percentage of activated cells and fluorescence intensity. There was a significant positive correlation between the number of epithelial apoptotic cells and fluorescence intensity. Increased neutrophil oxidative burst stimulated by H. pylori may play a role in enhanced gastric mucosal DNA damage and consequent atrophic gastritis and gastric cancer.
authors.forename: I
authors.initials: I
authors.lastname: Mizuki
authors.forename: T
authors.initials: T
authors.lastname: Shimoyama
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.affiliation: First Department of Internal Medicine and *Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.affiliation: First Department of Internal Medicine and *Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakaji
authors.forename: A
authors.initials: A
authors.lastname: Munakata
mesh_terms: D017209:Apoptosis; D001706:Biopsy; D004847:Epithelial Cells / Q000382:microbiology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology*; D005757:Gastritis, Atrophic / Q000382:microbiology / Q000473:pathology; D005838:Genotype; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism / Q000382:microbiology*; D011446:Prospective Studies; D016897:Respiratory Burst