title: In-vitro spermatogenesis resumption in men with maturation arrest: relationship with in-vivo blocking stage and serum FSH.
abstract: We have shown previously that germ cells recovered from some men with maturation arrest can resume spermatogenesis in vitro and give rise to late elongated spermatids. This study relates the ability of germ cells to differentiate in vitro to the stage at which spermatogenesis is blocked in vivo and to the patient's serum FSH concentration. The presence of germ cells at different stages of spermatogenesis was assessed, before and after culture, by classical cytology, by fluorescence in-situ hybridization and by immunocytochemistry with a germline-specific marker. The proportion of cases of maturation arrest at the primary spermatocyte, secondary spermatocyte and spermatid stage in which in-vitro resumption of meiosis was achieved was 24.3% (9/37), 100% (3/3) and 51.1% (23/45) respectively. Serum FSH concentrations were higher than normal in most cases. However, lower values were measured in patients in whom in-vitro spermatogenesis was achieved compared with those in whom no progression was detected. These data show that, under the conditions of this study, germ cells from men with very high serum FSH concentrations (>20 IU/l) are less likely to resume spermatogenesis in vitro than those coming from men with only moderate increase (10-20 IU/l).
authors.affiliation: Laboratoire d'Eylau, 55 rue Saint-Didier, 75116 Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Tesarik
authors.forename: B
authors.initials: B
authors.lastname: Balaban
authors.forename: A
authors.initials: A
authors.lastname: Isiklar
authors.forename: C
authors.initials: C
authors.lastname: Alatas
authors.forename: B
authors.initials: B
authors.lastname: Urman
authors.forename: S
authors.initials: S
authors.lastname: Aksoy
authors.forename: C
authors.initials: C
authors.lastname: Mendoza
authors.forename: E
authors.initials: E
authors.lastname: Greco
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology*; D003584:Cytological Techniques; D005640:Follicle Stimulating Hormone / Q000097:blood*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000097:blood / Q000503:physiopathology*; D009994:Osmolar Concentration; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D005640:Follicle Stimulating Hormone