title: Purification of recombinant calpastatin expressed in Escherichia coli.
authors.affiliation: Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Maki
authors.forename: K
authors.initials: K
authors.lastname: Hitomi
mesh_terms: D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification*; D002154:Calpain / Q000037:antagonists & inhibitors; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D015853:Cysteine Proteinase Inhibitors / Q000235:genetics* / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D011489:Protein Denaturation; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D002135:Calcium-Binding Proteins; D015853:Cysteine Proteinase Inhibitors; D011994:Recombinant Proteins; C033668:calpastatin; D002154:Calpain