Rapid genotype analysis of the matrix metalloproteinase-1 gene 1G/2G polymorphism that is associated with risk of cancer.
A bi-allelic polymorphism in the promoter of the human matrix metalloproteinase-1 gene has been reported. It has been found to have a functional effect on the promoter strength and to be associated with risk of cancers. The polymorphism is due to an insertion/deletion of a guanine, and conventional methodologies for genotyping this polymorphism are time-consuming and expensive. A rapid genotyping method based on restriction endonuclease digestion is reported here.
Dunleavey
L
L
Department of Human Genetics, University of Southampton, UK.
Beyzade
S
S
Ye
S
S
D005838:Genotype; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000145:classification / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D012307:Risk Factors
D020781:Matrix Metalloproteinase 1