Fluorescence line-narrowing detection in chromatography and electrophoresis.
A review of the basic aspects of fluorescence line-narrowing spectroscopy (FLNS) and its coupling with thin-layer chromatography (TLC) and polyacrylamide gel electrophoresis (PAGE) for off-line high-resolution low temperature spectral characterization is discussed. This is followed by a description of the on-line interfacing of capillary electrophoresis (CE) and capillary electrochromatography (CEC) with FLN detection. CE/ CEC-FLNS instrumentation and its applications for spectral identification of closely related analytes are also presented. Future prospects of micro and capillary high performance liquid chromatography (HPLC) with on-line high-resolution low temperature spectroscopic identification are considered.
Jankowiak
R
R
Department of Chemistry, Iowa State University, Ames 50011, USA. jankowiak@ameslab.gov
Roberts
K P
KP
Small
G J
GJ
D002855:Chromatography, Thin Layer / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods*