title: Molecular models of the structural arrangement of subunits and the mechanism of proton translocation in the membrane domain of F(1)F(0) ATP synthase.
abstract: Subunit c of the proton-transporting ATP synthase of Escherichia coli forms an oligomeric complex in the membrane domain that functions in transmembrane proton conduction. The arrangement of subunit c monomers in this oligomeric complex was studied by scanning mutagenesis. On the basis of these studies and structural information on subunit c, different molecular models for the potential arrangement of monomers in the c-oligomer are discussed. Intersubunit contacts in the F(0) domain that have been analysed in the past by chemical modification and mutagenesis studies are summarised. Transient contacts of the c-oligomer with subunit a might play a crucial role in the mechanism of proton translocation. Schematic models presented by several authors that interpret proton transport in the F(0) domain by a relative rotation of the c-subunit oligomer against subunit a are reviewed against the background of the molecular models of the oligomer.
authors.affiliation: Heinrich-Heine Universität Düssseldorf, Biochemie der Pflanzen, Universitätsstr. 1, 40225, Düsseldorf, Germany. georg.groth@uni-duesseldorf.de
authors.forename: G
authors.initials: G
authors.lastname: Groth
mesh_terms: D001389:Azirines / Q000737:chemistry; D002462:Cell Membrane / Q000201:enzymology; D002518:Ceramides / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D004926:Escherichia coli / Q000201:enzymology*; D008958:Models, Molecular; D008968:Molecular Conformation; D020409:Molecular Motor Proteins; D016296:Mutagenesis; D019920:Photoaffinity Labels; D006180:Proton-Translocating ATPases / Q000737:chemistry* / Q000235:genetics; D011522:Protons
chemical_list: C100592:3-trifluoromethyl-3-(3-iodophenyl)diazirineceramide; D001389:Azirines; D002518:Ceramides; D004220:Disulfides; D020409:Molecular Motor Proteins; D019920:Photoaffinity Labels; D011522:Protons; D006180:Proton-Translocating ATPases