Brain multiparametric responses to carbon monoxide exposure in the aging rat.
The multiparametric monitoring system was applied to study the effects of 2000 ppm carbon monoxide (CO) on brain functions in vivo in the aging rat. The vasodilatory (non hypoxic) effects of CO on CBF in normal adult rats, which were shown in concentrations of 1000-2000 ppm involved the effect of nitric oxide (NO). Energy metabolism was evaluated by optical monitoring of CBF and mitochondrial function by fluorometry of NADH. Ionic homeostasis was evaluated by monitoring the extracellular level of K(+) and H(+) and the DC steady potential. Seven aging rats (24 months) were exposed to 2000 ppm for 60 min and 120 min of recovery, while five control rats were exposed to air under the same conditions. A comparison between the CO group and the control group showed that the changes in CBF, NADH and light reflectance were not statistically significant while extracellular K(+) was elevated and tissue pH became more acidic. Thus, the typical CO induced increase in CBF, was not recorded in the aging rats. We concluded that the brain vasodilatory response to CO was not active in the aging rat, while the ionic homeostasis responses were similar to those found in the adult rat.
Mendelman
A
A
Faculty of Life Sciences, Bar-Ilan University, 52900, Ramat-Gan, Israel.
Zarchin
N
N
Rifkind
J
J
Mayevsky
A
A
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism; D002248:Carbon Monoxide / Q000494:pharmacology*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D010084:Oxidation-Reduction; D011188:Potassium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects
D002248:Carbon Monoxide; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium