title: Cockroach diuretic hormones: characterization of a calcitonin-like peptide in insects.
abstract: Insect diuretic hormones are crucial for control of water balance. We isolated from the cockroach Diploptera punctata two diuretic hormones (DH), Dippu-DH(31) and Dippu-DH(46), which increase cAMP production and fluid secretion in Malpighian tubules of several insect species. Dippu-DH(31) and -DH(46) contain 31 and 46 amino acids, respectively. Dippu-DH(46) belongs to the corticotropin-releasing factor (CRF)-like insect DH family, whereas Dippu-DH(31) has little sequence similarity to the CRF-like DH, but is similar to the calcitonin family. Dippu-DH(46) and -DH(31) have synergistic effects in D. punctata but have only additive effects in Locusta migratoria. Dippu-DH(31) represents a distinct type of insect DH with actions that differ from those of previously identified insect peptides with diuretic activity.
authors.affiliation: Department of Biochemistry, University of Nevada, Reno, NV 89557-0014, USA.
authors.forename: K
authors.initials: K
authors.lastname: Furuya
authors.forename: R J
authors.initials: RJ
authors.lastname: Milchak
authors.forename: K M
authors.initials: KM
authors.lastname: Schegg
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: S S
authors.initials: SS
authors.lastname: Tobe
authors.forename: G M
authors.initials: GM
authors.lastname: Coast
authors.forename: D A
authors.initials: DA
authors.lastname: Schooley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002116:Calcitonin / Q000302:isolation & purification* / Q000494:pharmacology; D003058:Cockroaches / Q000737:chemistry*; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D004232:Diuretics / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data
chemical_list: D004232:Diuretics; D002116:Calcitonin; D003346:Corticotropin-Releasing Hormone; D000242:Cyclic AMP