Rhinovirus-induced oxidative stress and interleukin-8 elaboration involves p47-phox but is independent of attachment to intercellular adhesion molecule-1 and viral replication.
Virus-induced elaboration of proinflammatory cytokines is mediated by virus-induced oxidative stress. The purpose of these studies was to determine the source of the virus-induced oxidative stress. Inhibition of viral replication with antibody to intercellular adhesion molecule-1 had no effect on virus-induced oxidative stress or interleukin-8 (IL-8) response (597+/-88 vs. 668+/-78 pg/mL in control cells). Treatment of cells with diphenylene iodonium inhibited virus-induced oxidative stress and IL-8 elaboration, but allopurinol, ibuprofen, and rotenone had no effect. Studies in cell lines produced from a patient with gp91-phox deficiency revealed normal responses. In contrast, the oxidative response was decreased and the IL-8 concentration was 227+/-36 pg/mL in cells from a patient with p47-phox deficiency, compared with 664+/-48 pg/mL in control cells. These studies suggest that the stimulation of reactive oxygen species by viral challenge occurs at the cell surface even in the absence of viral replication and involves a flavoprotein that may act in concert with p47-phox.
Kaul
P
P
Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USA.
Biagioli
M C
MC
Singh
I
I
Turner
R B
RB
D000493:Allopurinol / Q000494:pharmacology; D002395:Catecholamines / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology; D048288:Imidazolines; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology*; D016209:Interleukin-8 / Q000096:biosynthesis*; D019255:NADPH Oxidases; D018384:Oxidative Stress; D010750:Phosphoproteins / Q000502:physiology*; D012229:Rhinovirus / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism; D014779:Virus Replication
D002395:Catecholamines; D048288:Imidazolines; D016209:Interleukin-8; D010750:Phosphoproteins; D013481:Superoxides; D018799:Intercellular Adhesion Molecule-1; D000493:Allopurinol; C011775:(3,4-dihydroxyphenylamino)-2-imidazoline; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D007052:Ibuprofen