title: Chaetoatrosin A, a novel chitin synthase II inhibitor produced by Chaetomium atrobrunneum F449.
abstract: Chaetoatrosin A, a novel chitin synthase II inhibitor, was isolated from the culture broth of fungus F449, which was identified as Chaetomium atrobrunneum F449. Chaetoatrosin A was purified by solvent partition, silica gel, ODS, preparative TLC, and Sephadex LH-20 column chromatographies, consecutively. The structure of chaetoatrosin A was assigned as 1,8-dihydroxy-3(2-hydroxypropionyl)-6-methoxynaphthalene on the basis of various spectroscopic analyses including UV, IR, mass spectral, and NMR. Its molecular weight and formula were found to be 262 and C14H14O5, respectively. ,Chaetoatrosin A inhibited chitin synthase II by 50% at the concentration of 104 microg/ml in an enzyme assay system. This compound showed antifungal activities against Rhizoctonia solani, Pyricularia oryzae, Botrytis cinerea, Cryptococcus neoformans and Trichophyton mentagrophytes.
authors.affiliation: Antibiotics Research Lab, Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon, Korea.
authors.forename: E I
authors.initials: EI
authors.lastname: Hwang
authors.forename: B S
authors.initials: BS
authors.lastname: Yun
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: B M
authors.initials: BM
authors.lastname: Kwon
authors.forename: H G
authors.initials: HG
authors.lastname: Kim
authors.forename: H B
authors.initials: HB
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Bae
authors.forename: S U
authors.initials: SU
authors.lastname: Kim
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D002597:Chaetomium / Q000145:classification / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002687:Chitin Synthase / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation; D005658:Fungi / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009181:Mycoses / Q000382:microbiology; D009284:Naphthols / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D009284:Naphthols; C409193:chaetoatrosin A; D002687:Chitin Synthase