title: Optimization of simultaneous saccharification and fermentation for the production of ethanol from lignocellulosic biomass.
abstract: Simultaneous saccharification and fermentation (SSF) of alkaline hydrogen peroxide pretreated Antigonum leptopus (Linn) leaves to ethanol was optimized using cellulase from Trichoderma reesei QM-9414 (Celluclast from Novo) and Saccharomyces cerevisiae NRRL-Y-132 cells. Response surface methodology (RSM) and a three-level four-variable design were employed to evaluate the effects of SSF process variables such as cellulase concentration (20-100 FPU/g of substrate), substrate concentration (5-15% w/v), incubation time (24-72 h), and temperature (35-45 degrees C) on ethanol production efficiency. Cellulase and substrate concentrations were found to be the most significant variables. The optimum conditions arrived at are as follows: cellulase = 100 FPU/g of substrate, substrate = 15% (w/v), incubation time = 57.2 h, and temperature = 38.5 degrees C. At these conditions, the predicted ethanol yield was 3.02% (w/v) and the actual experimental value was 3.0% (w/v).
authors.affiliation: Biotechnology Division, Department of Chemical Engineering, Andhra University, Visakhapatnam 530 003, India. krishna70@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Hari Krishna
authors.forename: G V
authors.initials: GV
authors.lastname: Chowdary
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology
chemical_list: C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; D002480:Cellulase