Amniotic membrane transplantation or conjunctival limbal autograft for limbal stem cell deficiency induced by 5-fluorouracil in glaucoma surgeries.
OBJECTIVE
To determine if human amniotic membrane transplantation or limbal stem cell transplantation is effective to restore the corneal surface with partial or total limbal stem cell deficiency, respectively, caused by 5-fluorouracil (5-FU) toxicity after glaucoma surgeries.


METHODS
Partial and total limbal stem cell deficiency was confirmed by impression cytology as the cause of reduced vision and corneal surface breakdown in a 69-year-old man and a 67-year-old man, respectively, who both had received a total of 105 mg 5-FU injections. Amniotic membrane transplantation or conjunctival limbal autograft was performed for corneal surface reconstruction, respectively.


RESULTS
For a period of 15 months of follow-up, the visual acuity improved, and their corneal surfaces remained avascular, smooth, and without recurrence of limbal stem cell deficiency.


CONCLUSIONS
Limbal stem cell deficiency can occur as a late complication for patients receiving 5-FU after glaucoma filtering surgeries. Partial limbal stem cell deficiency can be treated with amniotic membrane transplantation alone, whereas limbal transplantation must be considered as an alternative for total limbal stem cell deficiency to restore the corneal surface integrity and vision.
Pires
R T
RT
Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida 33136, USA.
Chokshi
A
A
Tseng
S C
SC
D000368:Aged; D000650:Amnion / Q000637:transplantation*; D017690:Cell Transplantation; D003228:Conjunctiva / Q000637:transplantation*; D003316:Corneal Diseases / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation*; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000473:pathology; D015590:Fluorophotometry; D005472:Fluorouracil / Q000009:adverse effects*; D005902:Glaucoma, Open-Angle / Q000601:surgery*; D006801:Humans; D007429:Intraocular Pressure; D016850:Limbus Corneae / Q000187:drug effects* / Q000473:pathology; D008297:Male; D013234:Stem Cells / Q000187:drug effects* / Q000473:pathology; D014130:Trabeculectomy; D014182:Transplantation, Autologous; D014792:Visual Acuity
D005472:Fluorouracil