In vivo subcellular target compartments of interferon-gamma and interferon-gamma receptor (alpha- and beta-chains) in mouse liver.
IFN-gamma displays several effects on different tissues via its specific cell surface receptor (IFN-gammaR). In order to identify the target compartments of IFN-gamma and IFN-gammaR (alpha and beta-chains), we used a quantitative immunogold approach. In physiological conditions, IFN-gamma and IFN-gammaR immunoreactivities were detected in the plasma membrane, in the endoplasmic reticulum area, in the mitochondria and in the nucleus. After a single IFN-gamma injection, we observed, in a quantitative manner, an increase of signal density without modification of the subcellular distribution of IFN-gamma and IFN-gammaR subunits.
Lambert
A
A
CNRS UMR 5578, Université Claude Bernard-Lyon 1, Villeurbanne, France.
Sadir
R
R
Brisson
C
C
Morel
G
G
D000818:Animals; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000276:immunology* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000276:immunology / Q000648:ultrastructure; D007371:Interferon-gamma / Q000032:analysis / Q000493:pharmacokinetics*; D008099:Liver / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D016253:Microscopy, Immunoelectron; D008930:Mitochondria, Liver / Q000276:immunology / Q000648:ultrastructure; D017471:Receptors, Interferon / Q000032:analysis / Q000378:metabolism*; D011994:Recombinant Proteins; D000096743:Interferon gamma Receptor
D007371:Interferon-gamma; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D000096743:Interferon gamma Receptor