Transposition in Lactobacillus sakei: inactivation of a second lactocin S operon by the insertion of IS1520, a new member of the IS3 family of insertion sequences.
The analysis of spontaneous bacteriocin-negative mutants has led to the identification and characterization of a new, transpositionally active, insertion sequence of the IS3 family in the lactocin-S-producing Lactobacillus sakei strain L45. The element, which has been designated IS1520, is 1302 bp long with 10 bp perfect inverted repeat ends and generates direct repeats of a trinucleotide of target sequence upon transposition to the lactocin S locus. IS1520 encodes two consecutive, partially overlapping, major ORFs, which are frameshifted in a manner typical of the IS3 family. Despite a low overall DNA sequence similarity, the putative frameshifting region of IS1520 is highly similar to the corresponding region of IS1163, which is a related element previously shown to be active in L. sakei L45.
Skaugen
Morten
M
Laboratory of Microbial Gene Technology, Agricultural University of Norway, PO Box 5051, N-1432 Ås-NLH, Norway1.
Nes
Ingolf F
IF
Laboratory of Microbial Gene Technology, Agricultural University of Norway, PO Box 5051, N-1432 Ås-NLH, Norway1.
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001430:Bacteriocins; D001483:Base Sequence; D004251:DNA Transposable Elements; D020868:Gene Silencing; D007778:Lactobacillus / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D009876:Operon; D010455:Peptides; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004251:DNA Transposable Elements; D010455:Peptides; C103925:lactocin S