title: Development of a purification procedure for the isolation of nucleosides from urine prior to mass spectrometric analysis.
abstract: A chromatographic separation of nucleosides from urine has been developed in order to facilitate their mass spectrometric analysis for clinical diagnosis. A number of chromatographic resins were studied in order to develop an effective and efficient purification procedure. The optimized sequential protocol comprises a centrifugation, acidification and neutralization step, followed by application of an affinity chromatographic column and finally further separation on an acidic cation exchange column and a basic anion exchanger. This scheme shows effective clean-up of a standard radiolabelled nucleoside with a recovery of 92.5%, and recovery of nucleosides added to urine samples before extraction showed recoveries of 72-82%.
authors.affiliation: Biochemistry Research Group, School of Biological Sciences, University of Wales Swansea, UK.
authors.forename: E
authors.initials: E
authors.lastname: Dudley
authors.forename: S
authors.initials: S
authors.lastname: El-Shakawi
authors.forename: D E
authors.initials: DE
authors.lastname: Games
authors.forename: R P
authors.initials: RP
authors.lastname: Newton
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003911:Dextrans / Q000737:chemistry; D005782:Gels; D006801:Humans; D007475:Ion Exchange Resins / Q000737:chemistry; D013058:Mass Spectrometry; D009705:Nucleosides / Q000302:isolation & purification* / Q000652:urine
chemical_list: D000180:Acrylic Resins; D003911:Dextrans; D005782:Gels; D007475:Ion Exchange Resins; D009705:Nucleosides; C016680:polyacrylamide gels; C025614:sephadex