title: Haem oxygenase activity in human umbilical cord and rat vascular tissues.
abstract: Carbon monoxide (CO) has been shown to affect vascular tone in smooth muscle cells and thus, may regulate regional or systemic blood pressure as well as fetoplacental vascular tone and fetal blood delivery. To assess the potential of vascular tissue to produce CO, we determined haem oxygenase (HO) activity through in vitro quantitation of CO production with gas chromatography and its inhibition by 33-66 microm of chromium mesoporphyrin (CrMP) in homogenate preparations of rat aorta and vena cava and human umbilical cord tissues. We compared these results to HO activity in rat heart and liver. We also discuss normalization of HO activity on a per mg protein as well as per g fresh weight (FW) tissue basis. We found that both rat vascular tissue HO activities (per g FW) were equal, but greater than that of heart (x3) and less than that of liver (x0.2). For human cord tissues, HO activities of artery and vein were equal, but greater than that of Wharton's jelly. Also, HO activity in rat vascular tissues was 3x greater than that of the human cord tissues. HO activity was completely inhibited by CrMP in rat heart (90 per cent) and liver (96 per cent), but incompletely (50-66 per cent) in both rat and human vascular tissues. We established that it is unlikely that other non-haem CO-generating processes account for this unique insensitivity of HO to CrMP inhibition. In fact, high concentrations of other potent metalloporphyrin inhibitors affected vascular tissue HO even less. We found that the degree of in vitro HO inhibition appeared to be related to the concentration of haem in the reaction medium. We conclude that the presence of HO activity in cord tissues supports the possibility that CO plays a role in fetoplacental blood flow regulation.
authors.affiliation: Department of Pediatrics, Division of Neonatal and Developmental Medicine, Stanford University School of Medicine, Stanford, CA, 94305-5119, USA. henk.vreman@stanford.edu
authors.forename: H J
authors.initials: HJ
authors.lastname: Vreman
authors.forename: R J
authors.initials: RJ
authors.lastname: Wong
authors.forename: E C
authors.initials: EC
authors.lastname: Kim
authors.forename: D C
authors.initials: DC
authors.lastname: Nabseth
authors.forename: G S
authors.initials: GS
authors.lastname: Marks
authors.forename: D K
authors.initials: DK
authors.lastname: Stevenson
mesh_terms: D000818:Animals; D001011:Aorta / Q000201:enzymology; D002248:Carbon Monoxide / Q000502:physiology; D002857:Chromium; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D008099:Liver / Q000201:enzymology*; D008297:Male; D008665:Metalloporphyrins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D014469:Umbilical Arteries / Q000201:enzymology*; D014470:Umbilical Cord / Q000201:enzymology*; D014471:Umbilical Veins / Q000201:enzymology*; D014684:Venae Cavae / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D008665:Metalloporphyrins; D002857:Chromium; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing)