title: Expression and purification of woodchuck tumour necrosis factor alpha.
abstract: The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-alpha) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-alpha was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-alpha was purified and used for the production of neutralizing antisera.
authors.affiliation: Institut für Virologie, Universitätsklinikum Essen, Hufelandstr. 55, Essen, 45122, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Lohrengel
authors.forename: M
authors.initials: M
authors.lastname: Lu
authors.forename: D
authors.initials: D
authors.lastname: Bauer
authors.forename: M
authors.initials: M
authors.lastname: Roggendorf
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004926:Escherichia coli; D005354:Fibrosarcoma; D007074:Immunoglobulin G; D007739:L Cells; D008392:Marmota; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D015183:Restriction Mapping; D014162:Transfection; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha