title: Retinoid receptors and the induction of apoptosis in canine osteosarcoma cells.
abstract: Retinoids, all-trans-retinoic acid (ATRA) and 9-cis-retinoic acid (9-cis-RA), induced morphological changes and apoptosis-like cell death characterized by cell shrinkage, chromatin condensation and nuclear disintegration in three canine osteosarcoma cells, OOS, HOS and POS, at a concentration of 10(-5) M. Both retinoid receptors, RARs and RXRs, were identified in these cells. 9-cis-RA bound to both the RXRs and the RARs, whereas ATRA bound to only the RARs in these cells. Those results indicate that the induction of apoptosis in canine osteosarcoma cells may be mediated by the specific control of RARs and RXRs.
authors.affiliation: Division of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: S H
authors.initials: SH
authors.lastname: Hong
authors.forename: E
authors.initials: E
authors.lastname: Ohashi
authors.forename: T
authors.initials: T
authors.lastname: Kadosawa
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: S
authors.initials: S
authors.lastname: Matsunaga
authors.forename: R
authors.initials: R
authors.lastname: Nishimura
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000077556:Alitretinoin; D000818:Animals; D017209:Apoptosis; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D012516:Osteosarcoma / Q000473:pathology / Q000662:veterinary*; D018168:Receptors, Retinoic Acid / Q000032:analysis* / Q000378:metabolism; D047488:Retinoid X Receptors; D014157:Transcription Factors / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology
chemical_list: D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D014157:Transcription Factors; D000077556:Alitretinoin; D014212:Tretinoin