SDS/PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes.
Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/PAGE followed by renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.
Sanecka-Obacz
M
M
Department of Physiological Chemistry, Medical Academy, Lublin, Lubartowska, Poland.
D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D047388:Casein Kinases; D002642:Chick Embryo; D017868:Cyclic AMP-Dependent Protein Kinases / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000302:isolation & purification; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification*; D012269:Ribosomal Proteins / Q000378:metabolism; D012270:Ribosomes / Q000201:enzymology* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D007527:Isoenzymes; D012269:Ribosomal Proteins; D012967:Sodium Dodecyl Sulfate; D011494:Protein Kinases; D047388:Casein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases