title: Development of noncarious cervical notch lesions in vitro.
abstract: OBJECTIVE
  The initiation and progression of noncarious cervical notch lesions (NCCL) continues to perplex clinicians worldwide and poses a considerable restorative challenge. The purpose of this brief communication is to report what is believed to be the first in vitro production of notch-shaped lesions in the cervical third of premolar teeth.
  
  
  METHODS
  The lesions, were produced by axial loading of selected permanent premolar teeth in a 10% aqueous solution of sulfuric acid over a period of 5 days, followed by immersion in water for 7 days.
  
  
  RESULTS
  Results revealed macroscopic and microscopic features similar to those observed in noncarious cervical lesions in vivo. The lesions were incidental findings while the authors were studying stress corrosion of enamel at low pH. Although much remains to be investigated regarding the etiology and pathogenesis of NCCL, axial loading and a corrosive environment may be implicated in these processes. The artificial lesions arose in clinically sound teeth, suggesting that there is no simple clinical examination to identify teeth at risk from NCCL.
  
  
  CONCLUSIONS
  The relationship between the development of NCCL and applied stress indicates that occlusal factors may play the most significant role in the initiation and progression of NCCL.
authors.affiliation: Unit of Operative Dentistry and Endodontology, University of Manchester Dental Hospital, United Kingdom.
authors.forename: S A
authors.initials: SA
authors.lastname: Whitehead
authors.forename: N H
authors.initials: NH
authors.lastname: Wilson
authors.forename: D C
authors.initials: DC
authors.lastname: Watts
mesh_terms: D001641:Bicuspid / Q000473:pathology; D003343:Corrosion; D003799:Dental Stress Analysis; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D018709:Statistics, Nonparametric; D013314:Stress, Mechanical; D013464:Sulfuric Acids; D014072:Tooth Abrasion / Q000209:etiology*; D019237:Tooth Cervix / Q000473:pathology* / Q000648:ultrastructure; D014077:Tooth Erosion / Q000209:etiology*
chemical_list: D013464:Sulfuric Acids; C033158:sulfuric acid