Chromatographic and immunochemical approaches to the analysis of the HIV protease inhibitor saquinavir in plasma.
The development of the HIV protease inhibitor saquinavir (Ro 31-8959) required a range of analytical methods for its measurement in biological fluids. This paper describes the development of isocratic, reverse-phase HPLC/UV methods for the routine measurement of plasma levels of the drug together with a more sensitive radioimmunoassay. The performance of the two assays is compared with that of an HPLC/MS/MS method previously published and has been shown to be satisfactory, with coefficients of variation of calibration standards and quality control samples within the usual outside limits of +/- 15%. The HPLC/UV method can be routinely applied for concentrations down to 10-20 ng/ml and a lower limit of quantification of 1 ng/ml from 1 ml of human plasma is possible. The radioimmunoassay was developed for the specific measurement of saquinavir concentrations in human, HIV-positive plasma samples and has a lower limit of quantification of 0.5-1.0 ng/ml. Some preliminary findings suggested that it might not be specific in rat plasma and no attempts have been made to quantify any nonclinical samples with this technique. If still greater sensitivity is required, recourse can be made to the HPLC/MS/MS assay.
Wiltshire
H R
HR
Roche Discovery Welwyn, Welwyn Garden City, Hertfordshire, United Kingdom. hugh.wiltshire@roche.com
Wiltshire
B G
BG
Clarke
A F
AF
Worth
E
E
Prior
K J
KJ
Tjia
J F
JF
D000906:Antibodies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D017320:HIV Protease Inhibitors / Q000097:blood*; D006801:Humans; D011786:Quality Control; D011849:Radioactive Tracers; D011863:Radioimmunoassay / Q000379:methods*; D015203:Reproducibility of Results; D019258:Saquinavir / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D014614:Vaccines, Synthetic / Q000737:chemistry
D000906:Antibodies; D017320:HIV Protease Inhibitors; D011849:Radioactive Tracers; D014614:Vaccines, Synthetic; D019258:Saquinavir