Sensitivity to ethanol-induced motor incoordination in 5-HT(1B) receptor null mutant mice is task-dependent: implications for behavioral assessment of genetically altered mice.
Neuromuscular impairment by ethanol likely involves complex effects on balance, gait, muscle strength, and other features of motor coordination. The present experiments showed that relative sensitivity to ethanol-induced motor impairment in serotonin 1B (5-HT(1B)) null mutant and control mice was task dependent. We found that ethanol-treated null mutant mice made fewer missteps on a balance beam than did ethanol-treated wild-type mice, and confirmed a previous finding of their lesser ethanol sensitivity in the grid test. The genotypes did not differ in ethanol sensitivity as measured by the screen test, static dowel, fixed-speed rotarod, accelerating rotarod, grip strength, or loss of righting reflex tests. These experiments suggest that within a behavioral domain, alternative tests of function are not equivalent, so multiple assessment tools should be used to avoid misinterpretation of gene function.
Boehm
S L
SL
Portland Alcohol Research Center, Veterans Affairs Medical Center, Oregon 97201, USA. boehms@ohsu.edu
Schafer
G L
GL
Phillips
T J
TJ
Browman
K E
KE
Crabbe
J C
JC
D000818:Animals; D001921:Brain / Q000187:drug effects; D000431:Ethanol / Q000493:pharmacokinetics / Q000633:toxicity*; D005260:Female; D005838:Genotype; D018737:Hand Strength; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D008818:Mice, Neurologic Mutants; D009048:Motor Skills / Q000187:drug effects*; D009949:Orientation / Q000187:drug effects; D004856:Postural Balance / Q000187:drug effects*; D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin / Q000235:genetics*
D044302:Receptor, Serotonin, 5-HT1B; D011985:Receptors, Serotonin; D000431:Ethanol