Protection of BALB/c mice against experimental Helicobacter pylori infection by oral immunisation with H pylori heparan sulphate-binding proteins coupled to cholera toxin beta-subunit.
The presence of Helicobacter pylori in the gastroduodenal mucosae is associated with chronic active gastritis, peptic ulcers and gastric cancers such as adenocarcinoma and low-grade gastric B-cell lymphoma. In response to the presence of antibiotic-resistant strains, the use of vaccines to combat this infection has become an attractive alternative. The present study used a murine model of infection by a mouse-adapted H. pylori strain to determine whether infection in BALB/c mice can be successfully eradicated by intragastric vaccination with H. pylori heparan sulphate-binding proteins (HSBP) covalently coupled to the beta-subunit of cholera toxin (CTB). It was shown that vaccination confers protection against exposure of BALB/c mice to the pathogen, as revealed by microbiological, histopathological and molecular methods.
Ruiz-Bustos
E
E
Sierra-Beltran
A
A
Romero
M J
MJ
Rodriguez-Jaramillo
C
C
Department of Marine Pathology and *Histology Laboratory, Center for Biological Research, La Paz, Baja California Sur 23000, Mexico.
Ascencio
F
F
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage; D001646:Bile / Q000276:immunology; D001779:Blood Coagulation Factors; D002352:Carrier Proteins / Q000276:immunology* / Q000378:metabolism; D002772:Cholera Toxin / Q000276:immunology* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000276:immunology / Q000382:microbiology; D016481:Helicobacter Infections / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006493:Heparin / Q000378:metabolism; D018928:Immunity, Mucosal; D007114:Immunization / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D012269:Ribosomal Proteins; D018074:Vaccines, Conjugate / Q000008:administration & dosage
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D001779:Blood Coagulation Factors; D002352:Carrier Proteins; D004269:DNA, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D016601:RNA-Binding Proteins; C104946:RPL29 protein, human; D012269:Ribosomal Proteins; D018074:Vaccines, Conjugate; D006493:Heparin; D002772:Cholera Toxin