Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus.
A blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions. A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
Echeverría
M G
MG
Area of Virology, Faculty of Veterinary Sciences, National University of La Plata, Argentina.
Nosetto
E O
EO
Etcheverrigaray
M E
ME
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001118:Argentina; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D011558:Herpesvirus 1, Suid / Q000276:immunology / Q000302:isolation & purification*; D006735:Horseradish Peroxidase / Q000737:chemistry; D009500:Neutralization Tests / Q000662:veterinary; D011557:Pseudorabies / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology; D014510:Urease / Q000737:chemistry
D000914:Antibodies, Viral; D006735:Horseradish Peroxidase; D014510:Urease