title: Molecular analysis of two genes, DD9A and B, which are expressed during the postmolt stage in the decapod crustacean Penaeus japonicus.
abstract: In decapod crustaceans, deposition of calcium carbonate crystals (calcification) in the exoskeleton takes place during the postmolt phase of the molt cycle. In an attempt to identify proteins which regulate the calcification process, the differential display technique was used to identify genes which were specifically expressed in the integument during the postmolt stage in the penaeid prawn Penaeus japonicus. One of the genes thus identified, named DD9A, was expressed in the epithelial cells of the tail fan. DD9A encoded a putative precursor of a secreted protein of 113 amino acids which exhibited sequence similarities to a group of crustacean and insect cuticular proteins, suggesting that DD9A was a protein component of the exoskeleton. Another gene, DD9B, which was also transcribed specifically during the postmolt period was identified based on its sequence similarity to DD9A. Potential roles of the DD9A protein in the calcification of the exoskeleton will be discussed.
authors.affiliation: Laboratory of Molecular Biology of Marine Organisms, University of Tokyo, Japan. toshi@ori.u-tokyo.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: P
authors.initials: P
authors.lastname: Persson
authors.forename: H
authors.initials: H
authors.lastname: Endo
authors.forename: M
authors.initials: M
authors.lastname: Kono
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003445:Crustacea / Q000196:embryology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D018978:Molting / Q000235:genetics*
chemical_list: D019476:Insect Proteins