Col2-GFP reporter marks chondrocyte lineage and chondrogenesis during mouse skeletal development.
Mice were generated in which a Col2-GFP transgene serves as a reporter for the chondrocyte lineage and for chondrogenesis in live embryos and newborn pups. Cells actively engaged in chondrogenesis were identified by confocal optical sectioning within their native environments in embryos and in thick tissue slices. Chondrocytes exhibiting GFP fluorescence were purified from rib cages by high-speed cell sorting of crude cell suspensions. Intensity of fluorescence correlated with biosynthesis of procollagen II in these cells. The use of these mice and their cells provides a novel approach for studying chondrocyte differentiation and chondrogenesis during skeletal development.
Grant
T D
TD
Research Center, Shriners Hospital for Children, Portland, OR 97201, USA.
Cho
J
J
Ariail
K S
KS
Weksler
N B
NB
Smith
R W
RW
Horton
W A
WA
D000818:Animals; D001842:Bone and Bones / Q000196:embryology*; D019070:Cell Lineage; D002469:Cell Separation; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis / Q000502:physiology*; D003094:Collagen / Q000235:genetics; D005260:Female; D005453:Fluorescence; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D003094:Collagen