Comparison of non-viral transfection methods in melanoma cell primary cultures.
Melanoma primary cultures were transiently transfected via electroporation and lipofection for comparison. Transfection efficiency was superior with electroporation (58+/-9%) as compared to lipofection (23+/-9%) as determined by enhanced green fluorescent plasmid (EGFP) transfection. Secretion of IL-2 persisted for up to 3 weeks after electroporation. The increase in sensitivity against immunologic effector cells by transfection with IL-2 was not significant. Our results show the feasibility of a gene transfer into primary human melanoma cells, different from retroviral transduction.
Micka
B
B
Department of Medicine, Rheinische Friedrich Wilhelms Universität, Bonn, Germany.
Trojaneck
B
B
Niemitz
S
S
Lefterova
P
P
Kruopis
S
S
Huhn
D
D
Wittig
B
B
Schadendorf
D
D
Schmidt-Wolf
I G
IG
D015703:Antigens, CD / Q000032:analysis; D002411:Cation Exchange Resins; D004337:Drug Carriers; D018274:Electroporation / Q000379:methods; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000032:analysis / Q000235:genetics*; D008055:Lipids; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008545:Melanoma; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D002411:Cation Exchange Resins; D004337:Drug Carriers; D007376:Interleukin-2; D008055:Lipids; C086724:Lipofectamine; D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins