DNA release by line-1 (L1) retrotransposon. Could it be possible?
We have verified the presence of line-1 retrotransposon (L1) in plasma DNA in 15/17 brain tumor (glioma) patients and in 6/6 healthy people by applying PCR amplification of part of the L1 5' end. The same samples were separately amplified for K-ras. Results suggested that L1 sequences are circulating throughout the body. We hypothesized the participation of transposable elements such as L1 in a putative DNA release mechanism.
Alves
G
G
Divisão de Genética, Instituto Nacional de Câncer, Rio de Janeiro, Brazil. gbrown@inca.org.br
Kawamura
M T
MT
Nascimento
P
P
Maciel
C
C
Oliveira
J A
JA
Teixeira
A
A
Carvalho
M da G
Mda G
D000328:Adult; D000368:Aged; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics*; D005260:Female; D005910:Glioma / Q000235:genetics*; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004273:DNA, Neoplasm