CNS voltage-dependent Na(+) channel expression and distribution in an undifferentiated and differentiated CNS cell line.
Upon serum removal, CAD-R1 cells undergo neurite outgrowth and an increase in voltage-dependent Na(+) current (VDNaC) density without changing their activation and inactivation properties. Insulin and endothelial cell growth supplement inhibited the increase in VDNaC density but not the neurite outgrowth. RI, RII, RIII Na(+) channel proteins were expressed in CAD-R1 cells. These proteins exhibited both similar and different distribution and clustering patterns which suggested the channel's structural differences play a role in channel distribution.
Castañeda-Castellanos
D R
DR
Hospital for Special Surgery, Department of Anesthesiology, 535 East 70th Street, New York, NY 10021, USA.
Cano
M
M
Wang
J K
JK
Corbett
A
A
Benson
D
D
Blanck
T J
TJ
Thornhill
W B
WB
Recio-Pinto
E
E
D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D008564:Membrane Potentials / Q000502:physiology*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism*
D016895:Culture Media, Serum-Free; D015222:Sodium Channels