A glycoprotein isolated from the sponge, Pachymatisma johnstonii, has anti-leishmanial activity.
A high anti-leishmanial activity was observed in an aqueous extract from the marine sponge Pachymatisma johnstonii, Bowerbank 1842 (Demospongiae, Geodiidae). Pachymatismin, a glycoprotein, was purified and shown to be a cytotoxic agent, which acts on promastigote and clinical-like amastigote stages with IC50 about 1 microg protein/ml and induces changes in the cell shape, phospholipase A2 activity and invasion capacity of the parasite. We believe pachymatismin is the first reported substance from a marine organism with anti-leishmanial activity.
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Unité de Parasitologie, UPRES 1155, Faculté de Pharmacie, Université de Nantes, France. plepape@sante.univ-nantes.fr
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Abdala
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D000818:Animals; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D036341:Intercellular Signaling Peptides and Proteins; D007891:Leishmania / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D017737:Macrophages, Peritoneal / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011161:Porifera / Q000737:chemistry*
D000981:Antiprotozoal Agents; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C106743:pachymatismin protein, Pachymatisma johnstonii; D010741:Phospholipases A; D054467:Phospholipases A2