Successful transplantation of highly selected CD34+ peripheral blood stem cells in a HLA-sensitized patient treated with immunoadsorption onto protein A.
BACKGROUND
Haploidentical bone marrow transplantation with preexisting anti-HLA antibodies is associated with a high risk of graft failure.


METHODS
A 27-year-old female patient with chronic myeloid leukemia and evidence of several osseous chloromas had no suitable matched bone marrow donor, and fluorescence cytometric cross-match (FCXM) revealed antibodies against donor-specific HLA-molecules. Immunoadsorption onto staphylococcal protein A was applied to remove these antibodies, and peripheral stem cell transplantation was performed from her haploidentical sister after a negative FCXM was documented after immunoadsorption and conditioning treatment.


RESULTS
FCXM for donor lymphocytes and stem cells remained negative throughout the posttransplant period, and engraftment of donor cells was documented on day +69.


CONCLUSIONS
Immunoadsorption onto protein A should be considered in stem cell transplantation even from an haploidentical donor where anti-HLA antibodies and a positive FCXM are documented.
Braun
N
N
Sektion Nieren- und Hochdruckkrankheiten der Abteilung III, Medizinische Universitätsklinik und Poliklinik, Tübingen, Germany. nbraun@uni-tuebingen.de
Faul
C
C
Wernet
D
D
Schnaidt
M
M
Stuhler
G
G
Kanz
L
L
Risler
T
T
Einsele
H
H
D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006680:HLA Antigens / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006650:Histocompatibility Testing; D006801:Humans; D007114:Immunization; D007163:Immunosorbent Techniques; D013205:Staphylococcal Protein A / Q000627:therapeutic use*; D013234:Stem Cells / Q000276:immunology*
D018952:Antigens, CD34; D006680:HLA Antigens; D013205:Staphylococcal Protein A