Routine analysis of short-chain fatty acids for anaerobic bacteria identification using capillary electrophoresis and indirect ultraviolet detection.
The diagnosis of anaerobes can be difficult to perform, using classical biochemical tests. Characterization of metabolic end-products such as short-chain fatty acids (SCFA) was often used because of their reproducible biosynthesis. Despite this, SCFA are difficult to study using gas chromatography, due to their high volatility. Furthermore, the treatment of the samples are long and fastidious. Capillary electrophoresis and indirect UV detection (CE-indirect UV) is a well-known analytical method to study inorganic or organic anions. In this work, we validate the analysis of SCFA using CE-indirect UV detection. To do this, we studied the culture media of 98 anaerobic strains for the detection and quantitation of the following acids: succinic, pyruvic, acetic, lactic, propionic, 2-hydroxybutyric, butyric, 2-hydroxyvaleric, isovaleric, isocaproic, and 3-phenylpropionic. We verified that the CE-indirect UV detection analysis of SCFA for taxonomical data can be used as a mean for rapid identification for the study of anaerobes.
Arellano
M
M
Laboratoire de Synthèse, Physico-Chimie, Radiobiologie, Service de Chimie Analytique, Faculté des Sciences Pharmaceutiques, Toulouse, France.
Jomard
P
P
El Kaddouri
S
S
Roques
C
C
Nepveu
F
F
Couderc
F
F
D001421:Bacteria, Anaerobic / Q000737:chemistry / Q000145:classification*; D015373:Bacterial Typing Techniques; D003470:Culture Media; D019075:Electrophoresis, Capillary / Q000379:methods*; D005232:Fatty Acids, Volatile / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D003470:Culture Media; D005232:Fatty Acids, Volatile