A high-resolution radiation hybrid map of the proximal portion of mouse chromosome 5.
Radiation hybrid (RH) mapping of the mouse genome provides a useful tool in the integration of existing genetic and physical maps, as well as in the ongoing effort to generate a dense map of expressed sequence tags. To facilitate functional analysis of mouse Chromosome 5, we have constructed a high-resolution RH map spanning 75 cM of the chromosome. During the course of these studies, we have developed RHBase, an RH data management program that provides data storage and an interface to several RH mapping programs and databases. We have typed 95 markers on the T31 RH panel and generated an integrated map, pooling data from several sources. The integrated RH map ranges from the most proximal marker, D5Mit331 (Chromosome Committee offset, 3 cM), to D5Mit326, 74.5 cM distal on our genetic map (Chromosome Committee offset, 80 cM), and consists of 138 markers, including 89 simple sequence length polymorphic markers, 11 sequence-tagged sites generated from BAC end sequence, and 38 gene loci, and represents average coverage of approximately one locus per 0.5 cM with some regions more densely mapped. In addition to the RH mapping of markers and genes previously localized on mouse Chromosome 5, this RH map places the alpha-4 GABA(A) receptor subunit gene (Gabra4) in the central portion of the chromosome, in the vicinity of the cluster of three other GABA(A) receptor subunit genes (Gabrg1-Gabra2-Gabrb1). Our mapping effort has also defined a new cluster of four genes in the semaphorin gene family (Sema3a, Sema3c, Sema3d, and Sema3e) and the Wolfram syndrome gene (Wfs1) in this region of the chromosome.
Tarantino
L M
LM
Center for Neurobiology and Behavior, Department of Neuroscience, Department of Genetics, University of Pennsylvania, 111 CRB, 415 Curie Boulevard, Philadelphia, Pensylvania
Feiner
L
L
Alavizadeh
A
A
Wiltshire
T
T
Hurle
B
B
Ornitz
D M
DM
Webber
A L
AL
Raper
J
J
Lengeling
A
A
Rowe
L B
LB
Bucan
M
M
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002630:Chemotactic Factors / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002895:Chromosomes, Human, Pair 5 / Q000235:genetics; D016208:Databases, Factual; D005819:Genetic Markers; D005981:Glutathione Synthase / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D005810:Multigene Family / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D015221:Potassium Channels / Q000235:genetics; D024683:Potassium Channels, Tandem Pore Domain; D011963:Receptors, GABA-A / Q000235:genetics; D040121:Semaphorin-3A; D016324:Sequence Tagged Sites; D012984:Software
D002352:Carrier Proteins; D002630:Chemotactic Factors; D005819:Genetic Markers; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D011963:Receptors, GABA-A; C108639:SEMA3A protein, human; C488217:Sema3a protein, mouse; D040121:Semaphorin-3A; C420132:potassium channel subfamily K member 3; D005981:Glutathione Synthase