title: 14-3-3 proteins interact with a 13-lipoxygenase, but not with a 9-lipoxygenase.
abstract: Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.
authors.affiliation: Center for Phytotechnology UL-TNOO, Department of Applied Plant Sciences, Wassenaarseweg 64, 2333 Al Leiden, The Netherlands. holtman@voeding.tno.nl
authors.forename: W L
authors.initials: WL
authors.lastname: Holtman
authors.forename: M R
authors.initials: MR
authors.lastname: Roberts
authors.forename: B J
authors.initials: BJ
authors.lastname: Oppedijk
authors.forename: C
authors.initials: C
authors.lastname: Testerink
authors.forename: M J
authors.initials: MJ
authors.lastname: van Zeijl
authors.forename: M
authors.initials: M
authors.lastname: Wang
mesh_terms: D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004926:Escherichia coli; D001467:Hordeum / Q000201:enzymology; D007163:Immunosorbent Techniques; D007527:Isoenzymes / Q000378:metabolism*; D008084:Lipoxygenase / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance; D014446:Tyrosine 3-Monooxygenase
chemical_list: D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011506:Proteins; D011994:Recombinant Proteins; C117433:13-lipoxygenase; D008084:Lipoxygenase; D014446:Tyrosine 3-Monooxygenase