A chromogenic medium for the detection of yeasts with beta-galactosidase and beta-glucosidase activities from intermediate moisture foods.
A selective and differential solid medium for the specific detection of some common yeasts frequently causing spoilage in intermediate moisture foods is described. The principle of the method is based on the detection of two enzymes, beta-glucosidase and beta-galactosidase, using the chromogenic substrates salmon-Gluc and X-Gal. Over 140 yeasts and bacteria were tested, and Debaryomyces hansenii and Kluyveromyces marxianus strains produced salmon and dark blue colonies, respectively, thus permitting their clear discrimination from other yeasts common in intermediate moisture foods. The medium was very satisfactory when intermediate moisture foods were tested.
de Silóniz
M I
MI
Departamento de Microbiología, Facultad de Biología, Universidad Complutense de Madrid, Spain. siloniz@eucmax.sim.ucm.es
Valderrama
M J
MJ
Peinado
J M
JM
D002863:Chromogenic Compounds; D003470:Culture Media; D005516:Food Microbiology; D007716:Kluyveromyces / Q000201:enzymology; D008828:Microbiological Techniques; D004718:Saccharomycetales / Q000201:enzymology; D015003:Yeasts / Q000201:enzymology*; D001616:beta-Galactosidase / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
D002863:Chromogenic Compounds; D003470:Culture Media; D001617:beta-Glucosidase; D001616:beta-Galactosidase