title: Localisation and accumulation of a new carotenoporphyrin in two primary tumour models.
abstract: We have investigated the tumour-localising properties and in vivo fluorescence kinetics of a hexamethoxylated carotenqporphyrin (CP6) in two primary tumour models: UV-B-induced early skin cancer in hairless mice and chemically induced mucosal dysplasia in the rat palate. CP6 fluorescence kinetics are investigated by measuring in vivo fluorescence spectra and images of the mouse skin and the rat palate at different time points after injection. For the tumour-localising properties, microscopic phase-contrast and fluorescence images are recorded. The in vivo fluorescence kinetics in the mouse skin show localization of CP6 in the tumours. However, fluorescence microscopy images show that CP6 localises in the dermis and structures that are not related to the malignant transformation of the mouse skin. The fluorescence kinetics in the rat palate show a significant correlation between the degree of malignancy and the CP6 fluorescence build-up time in the palate. The microscopic images show that CP6 fluorescence localises in the connective tissue and not in the dysplastic epithelium. In conclusion, CP6 does not localise preferentially in (pre-) cancerous tissue in the two primary tumour models studied here, in contrast to reports about localisation of carotenoporphyrins in transplanted tumours. However, the CP6 build-up time in rat palates correlates with the degree of malignancy and this might possibly be a useful parameter in tumour detection.
authors.affiliation: Department of Radiation Oncology, Daniel den Hoed Cancer Centre, University Hospital Rotterdam, The Netherlands.
authors.forename: J T
authors.initials: JT
authors.lastname: van den Akker
authors.forename: O C
authors.initials: OC
authors.lastname: Speelman
authors.forename: H J
authors.initials: HJ
authors.lastname: van Staveren
authors.forename: A L
authors.initials: AL
authors.lastname: Moore
authors.forename: T A
authors.initials: TA
authors.lastname: Moore
authors.forename: D
authors.initials: D
authors.lastname: Gust
authors.forename: W M
authors.initials: WM
authors.lastname: Star
authors.forename: H J
authors.initials: HJ
authors.lastname: Sterenborg
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D015394:Molecular Structure; D010157:Palatal Neoplasms / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012878:Skin Neoplasms / Q000378:metabolism*
chemical_list: D011166:Porphyrins; C090538:trimethoxycarotenoporphyrin; D002338:Carotenoids