Interaction of exogenous DNA with the nuclear matrix of live spermatozoa.
Sperm chromatin is a highly organized array of protamines and DNA, with the protamines serving to tightly condense the DNA into a compact, defined structure. We have previously demonstrated that the sperm nucleus is an ordered library of DNA organized into functional zones, such as the nuclear matrix and nuclear annulus. Other laboratories have suggested that mouse spermatozoa can interact with exogenous pSV2CAT plasmid DNA. In this work, we explored this interaction and examined the subcellular localization of the exogenous DNA. We found a repeatable association of exogenous DNA with a specific region of the sperm nuclear matrix. This region of the nucleus correlates with the equatorial segment of the sperm head. This interaction requires only a defined fertilization media, transfection quality DNA, and incubation with spermatozoa.
McCarthy
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Rutgers University, Division of Urology, Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA.
Ward
W S
WS
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015530:Nuclear Matrix / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure; D014162:Transfection
D002843:Chromatin; D004247:DNA