title: Absence of shedding of two B. abortus strains in goats after vaccination with live vaccines.
abstract: The objective of this study was to determine if B. abortus rough mutant strains RB51 and rfbK are eliminated in goat milk. Thirty milk goats were divided into two groups. Group I was inoculated with 4x10(10) cfu/ml of B.abortus RB51 strain and Group II with 1x10(9) cfu/ml of B. abortus rfbK strain by subcutaneous route in the right axilary region. Milk samples were taken aseptically on a daily basis for the first 30 days and weekly for the second and third months. The samples were inoculated on Farrell's media and incubated under 5-10% CO(2) at 37 degrees C for 10 days. The suspicious colonies were recultured in Farrell's media. There were no isolations of bacteria with characteristics of Brucella from any of the milk samples collected during 90 days of the study. It was concluded that neither of the strains used at these doses were eliminated by milk in goats inoculated during lactation.
authors.affiliation: Centro de Enseñanza, Práctica, Investigación y Extensionismo en Rumiantes (CEPIER), Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, D.F. 04510, Coyoacán, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Soberón-Mobarak
authors.forename: E
authors.initials: E
authors.lastname: Díaz-Aparicio
authors.forename: J
authors.initials: J
authors.lastname: Torres-Armenta
authors.forename: L G
authors.initials: LG
authors.lastname: Adams
authors.forename: F
authors.initials: F
authors.lastname: Suárez-Güemes
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology* / Q000302:isolation & purification; D006041:Goats; D008892:Milk / Q000382:microbiology; D009154:Mutation; D014611:Vaccination
chemical_list: D001428:Bacterial Vaccines