title: Purification and kinetic characterization of an anionic peroxidase from melon (Cucumis melo L.) cultivated under different salinity conditions.
abstract: The partial characterization of an anionic peroxidase in melon fruit is described. Four melon peroxidase (MPX) isoenzymes were detected in crude extracts after isoelectric focusing. The major MPX isoenzyme (pI = 3.7) was partially purified by including hydrophobic and anion-exchange chromatography in the purification scheme. The sample obtained was used to characterize MPX. This peroxidase did not show activity on ascorbic acid but oxidized guaiacol at a high rate, showing an optimum pH of 5.5 when acting on this last reducing substrate. Melon fruits grown under highly saline conditions showed slightly increased levels of this anionic isoenzyme. Kinetic studies using 2,2'-azinobis(3-ethylbenzothiazolinesulfonic acid) (ABTS) as reducing substrate showed that increased salinity in the growth medium did not modify the kinetic parameters of melon peroxidase on both hydrogen peroxide and reducing substrate.
authors.affiliation: Grupo de Investigación de Enzimología (GENZ), Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain.
authors.forename: J N
authors.initials: JN
authors.lastname: Rodríguez-López
authors.forename: J C
authors.initials: JC
authors.lastname: Espín
authors.forename: F
authors.initials: F
authors.lastname: del Amor
authors.forename: J
authors.initials: J
authors.lastname: Tudela
authors.forename: V
authors.initials: V
authors.lastname: Martínez
authors.forename: A
authors.initials: A
authors.lastname: Cerdá
authors.forename: F
authors.initials: F
authors.lastname: García-Cánovas
mesh_terms: D019662:Cucurbitaceae / Q000201:enzymology*; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D010544:Peroxidases / Q000302:isolation & purification* / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry*
chemical_list: D007527:Isoenzymes; D012965:Sodium Chloride; D010544:Peroxidases; C120494:anionic peroxidase