Effects of metabolites of benzo(a)pyrene on unschedule DNA synthesis in BALB/3T3 cell line.
In order to explore the damage from metabolites of benzo(a)pyrene on DNA of mammalian cells, the effects of four metabolites of benzo(a)pyrene (anti-BPDE, syn-BPDE, 3-OH-BP and 9-OH-BP) on synthesis of DNA and unschedule DNA synthesis (UDS) in BALB/3T3 cells were assayed, by methods of single-labeling and double-labeling. The results showed that all of the four agents were able to increase the synthesis of DNA, but only three of them (apart from syn-BPDE) induced UDS in BALB/3T3 cells. The above indicates that the metabolites of benzo(a)pyrene are able to damage DNA in BALB/3T3 cells, and that this effect may be relative to the sterical structure of metabolites of benzo(a)pyrene.
Chen
J K
JK
Institute for Chemical Carcinogenesis, Guangzhou Medical College, People's Republic of China.
Wu
Z L
ZL
Liu
Y G
YG
Lei
Y X
YX
D016475:3T3 Cells; D000818:Animals; D001564:Benzo(a)pyrene / Q000009:adverse effects* / Q000031:analogs & derivatives / Q000494:pharmacology; D002273:Carcinogens / Q000009:adverse effects* / Q000494:pharmacology; D018929:Cell Culture Techniques; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008175:Lung Neoplasms / Q000139:chemically induced; D051379:Mice; D008807:Mice, Inbred BALB C; D018675:Toxicity Tests
D002273:Carcinogens; D001564:Benzo(a)pyrene; D004247:DNA