title: Elevated temperature greatly improves transformation of fresh and frozen competent cells in yeast.
authors.affiliation: Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University, Ube, Japan. rinji@po.cc.yamaguchi-u.ac.jp
authors.forename: R
authors.initials: R
authors.lastname: Akada
authors.forename: M
authors.initials: M
authors.lastname: Kawahata
authors.forename: Y
authors.initials: Y
authors.lastname: Nishizawa
mesh_terms: D015925:Cryopreservation; D004271:DNA, Fungal / Q000235:genetics; D006358:Hot Temperature; D008967:Molecular Biology / Q000379:methods*; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development
chemical_list: D004271:DNA, Fungal