Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric detection of bacterial biomarker proteins isolated from contaminated water, lettuce and cotton cloth.
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectra of bacterial proteins were obtained from water, lettuce and cloth samples contaminated with Shigella flexneri, Escherichia coli, and Aeromonas hydrophila. Spectra were obtained using proteins directly isolated from water (or water used for rinsing samples) without culturing the bacteria. For S. flexneri and E. coli, two marker ions for specific proteins associated with a virulence-related property (acid resistance) were easily detected. For A. hydrophila, ions from two specifically selected marker proteins, as well as ions from the larger group of proteins isolated from pure cultures, all matched spectra from a contaminated water sample, providing strong evidence that A. hydrophila was the bacterial contaminant. Rinse water from contaminated lettuce and cloth samples showed the same marker ions as the contaminated water samples.
Holland
R D
RD
National Center for Toxicological Research, Jefferson, AR 72079, USA.
Rafii
F
F
Heinze
T M
TM
Sutherland
J B
JB
Voorhees
K J
KJ
Lay
J O
JO
D016980:Aeromonas hydrophila / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D004926:Escherichia coli / Q000302:isolation & purification; D005516:Food Microbiology; D003368:Gossypium / Q000382:microbiology*; D007477:Ions; D007834:Lasers; D018545:Lactuca / Q000382:microbiology*; D012763:Shigella flexneri / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014871:Water Microbiology
D001426:Bacterial Proteins; D015415:Biomarkers; D007477:Ions