A highly sensitive, non-radioactive assay for T cell activation in cattle: applications in screening for antigens recognised by CD4(+) and CD8(+) T cells.
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNgamma and activation of immune CD4(+) and CD8(+) T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4(+) T cell clones.
Ballingall
K T
KT
International Livestock Research Institute, P.O. Box 30709, Nairobi, Kenya. k.ballingall@cgiar.org
Mwangi
D M
DM
MacHugh
N D
ND
Taracha
E L
EL
Totte
P
P
McKeever1
D J
DJ
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D016995:Ehrlichia ruminantium / Q000276:immunology*; D004730:Endothelium, Vascular / Q000166:cytology; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology; D007457:Iodine Radioisotopes; D008213:Lymphocyte Activation / Q000276:immunology*; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D016797:Theileria parva / Q000276:immunology*
D000953:Antigens, Protozoan; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; D007371:Interferon-gamma