title: Analysis of baclofen by capillary electrophoresis with laser-induced fluorescence detection.
abstract: A new analytical method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. Naphthalene-2,3-dicarboxaldehyde was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and a He-Cd laser (excitation at 442 nm, emission at 500 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the concentration limit of detection was in the nanomolar level. Coupled with a simple cleanup procedure, the method was successfully applied to the analysis of baclofen in human plasma. Recovery of spiked baclofen in plasma was 98%. The relative standard deviation values on peak size and migration time were 7.9% and 0.4%, respectively. The limit of detection of baclofen in plasma was 10 ng/ml.
authors.affiliation: Department of Chemistry, Tunghai University, Taichung, Taiwan.
authors.forename: M T
authors.initials: MT
authors.lastname: Chiang
authors.forename: S Y
authors.initials: SY
authors.lastname: Chang
authors.forename: C W
authors.initials: CW
authors.lastname: Whang
mesh_terms: D001418:Baclofen / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D018755:GABA Agonists / Q000032:analysis*; D006801:Humans; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D018755:GABA Agonists; D001418:Baclofen