Immunocytochemical characteristics of cells and fibers in the nasal mucosa of young and adult macaques.
The mammalian nasal cavity is lined by an olfactory mucosa (OM) and a respiratory mucosa (RM). The principal OM cell type is the olfactory receptor neuron (ORN). However, little is known about ORNs in the life histories of primates. The RM, similar to the RM in the tracheobronchial tract (TBT), is dominated by ciliated columnar cells. Neuroendocrine cells (NECs) are essential in the TBT; little is known about nasal NECs. This study examined the immunolabeling characteristics of primate OM and RM for three important proteins-calretinin (CR), olfactory marker protein (OMP), and protein gene product 9.5 (PGP). Tissues from newborn to 15-year-old macaques were analyzed to determine the expression of these proteins during various stages of development. Standard immunocytochemistry on aldehyde-fixed tissues was applied, utilizing the avidin-biotin peroxidase (ABC) method. Immuno-electron microscopy confirmed the immunoreactive cell types. ORNs were immunoreactive for CR, OMP, and PGP at all ages studied. Immunoreactivity for PGP also was displayed in a subset of ciliated, columnar epithelial cells in the RM and in an extensive network of subepithelial fibers spread throughout both mucosae. The results suggest that macaque ORNs express three important proteins over a wide life history, and that the macaque may be a reliable model for studying primate/human olfaction during aging. The PGP-labeling results also suggest that the macaque nasal peptidergic fibers express PGP and that the respiratory epithelium contains NECs with labeling characteristics similar to those in the TBT.
Johnson
E W
EW
Department of Biological Sciences, Idaho State University, Pocatello, 83209, USA. johnedwa@isu.edu
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D064032:Calbindin 2; D007150:Immunohistochemistry; D008251:Macaca / Q000033:anatomy & histology* / Q000254:growth & development; D016253:Microscopy, Immunoelectron; D009297:Nasal Mucosa / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis; D051842:Olfactory Marker Protein; D018034:Olfactory Receptor Neurons / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D013869:Thiolester Hydrolases / Q000032:analysis; D043222:Ubiquitin Thiolesterase
C576764:CALB2 protein, human; D064032:Calbindin 2; D009419:Nerve Tissue Proteins; D051842:Olfactory Marker Protein; D064030:S100 Calcium Binding Protein G; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase