title: Accumulation of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in porcine preovulatory follicles after in vitro exposure to TCDD: effects on steroid secretion and cell proliferation.
abstract: The present experiments were conducted to test 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) accumulation in the tissues, and its influence on cell proliferation and steroid secretion. A dose of 3.2 ng of TCDD/g tissue was added at the beginning of the culture, and the media were changed every 24 h or not changed till the end (96 h) of the culture. TCDD in the tissue was analysed by mass spectrometry, and the percentage of proliferating cells was measured using the MIB-1 labelling index. TCDD added to the culture medium accumulated in the tissues after 24 h (59.3%) and 96 h (81.2%) of exposure. The accumulative effect of TCDD was manifested by a reduction in the percentage of proliferating cells (53.5 and 33.8%, after 24 and 96 h exposure, respectively). A single exposure to TCDD had no effect on progesterone, reduced testosterone secretion and caused a significant increase in oestradiol secretion. Prolonged exposure to TCDD caused an increase in the concentration of the three steroids investigated in the culture medium. The results suggest that TCDD action is complex in the follicles.
authors.affiliation: Department of Chemistry, Cracow University of Technology, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Grochowalski
authors.forename: R
authors.initials: R
authors.lastname: Piekło
authors.forename: A
authors.initials: A
authors.lastname: Gasińska
authors.forename: R
authors.initials: R
authors.lastname: Chrzaszcz
authors.forename: E L
authors.initials: EL
authors.lastname: Gregoraszczuk
mesh_terms: D000728:Androgens / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004967:Estrogens / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D066298:In Vitro Techniques; D006080:Ovarian Follicle / Q000166:cytology* / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000493:pharmacokinetics*; D011374:Progesterone / Q000378:metabolism; D013552:Swine; D013723:Teratogens / Q000493:pharmacokinetics*
chemical_list: D000728:Androgens; D004967:Estrogens; D012739:Gonadal Steroid Hormones; D000072317:Polychlorinated Dibenzodioxins; D013723:Teratogens; D011374:Progesterone