Purification and structure of neurostatin, an inhibitor of astrocyte division of mammalian brain.
Neurostatin was originally described as an inhibitor of astroblast and astrocytoma division present in rat brain extracts and immunologically related to the sugar moiety of epidermal growth factor receptor and to blood group antigens. It was purified recently from mammalian brain extracts and characterized as a glycosphingolipid, but its precise structure remained unknown. Neurostatin has now been purified to apparent homogeneity from ganglioside extracts of rat, bovine, and porcine brain. It is cytostatic for astroblasts, C6 glioma cells, and various human astrocytomas grades III and IV, with IC(50) values ranging from 250 to 450 nM, but does not affect the division of primary or transformed fibroblasts up to concentrations >4 microM. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of purified pig neurostatin showed a molecular ion of 1, 905 Da and ions of 1,863 and 1,934 Da, compatible with a disialoganglioside. Mono- and bidimensional NMR spectra, together with biochemical studies, suggest that neurostatin may be the 9-O-monoacetyl ester of GD1b.
Abad-Rodríguez
J
J
Neural Plasticity Group, Instituto Cajal, Madrid, Spain. Institute of Organic Chemistry, CSIC, Madrid, Spain.
Bernabé
M
M
Romero-Ramírez
L
L
Vallejo-Cremades
M
M
Fernández-Mayoralas
A
A
Nieto-Sampedro
M
M
D016475:3T3 Cells; D000468:Alkalies / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000187:drug effects; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000494:pharmacology; D003636:DEAE-Cellulose; D005326:Fetal Proteins / Q000494:pharmacology; D005910:Glioma; D006028:Glycosphingolipids / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008322:Mammals; D051379:Mice; D019579:Neocortex / Q000737:chemistry* / Q000166:cytology; D009439:Neuraminidase / Q000494:pharmacology; D011522:Protons; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014407:Tumor Cells, Cultured
D000468:Alkalies; D003470:Culture Media; D005326:Fetal Proteins; D006028:Glycosphingolipids; D011522:Protons; C116059:neurostatin; D003636:DEAE-Cellulose; D009439:Neuraminidase