title: [A convenient method for laboratory synthesis of the N-([1-14C]palmitoyl)ethanolamine, labeled by a fatty acid].
abstract: Data concerning the synthesis of bioactive lipid compound N-([1-14C]-palmitoyl)ethanolamine labeled by 14C fatty acid are reported. The method is based on the ability of ethanolamine and fatty acid to the direct chemical condensation at 180 degrees C with yielding of N-acylethanolamine. The purification of the end product by the double crystallization in ethanol allows to obtain chromatographically pure substance. The presented method of the labeled N-([1-14C]-palmitoyl)ethanolamine synthesis is simple and in extensive that's why it might be used in the area of biologically active compounds investigation.
authors.affiliation: O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
authors.forename: V M
authors.initials: VM
authors.lastname: Marhitykh
authors.forename: O D
authors.initials: OD
authors.lastname: Zhukov
authors.forename: M V
authors.initials: MV
authors.lastname: Artamonov
authors.forename: N M
authors.initials: NM
authors.lastname: Hula
mesh_terms: D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D010714:Phosphatidylethanolamines / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D002250:Carbon Radioisotopes; D010714:Phosphatidylethanolamines