Interference of thimerosal in the bicinchoninic acid protein microassay.
In this investigation the total amount of protein present in the three influenza vaccine formulations Fluvirin, Fluzone and Flushield have been tested where thimerosal is present as a preservative. Interaction of thimerosal with protein has been confirmed by analysing ovalbumin as a control against ovalbumin-thimerosal solution. The results indicate that interference of thimerosal can be overcame for the quantitative assay of proteins by BCA technique with the good reproducibility. The present study identified also the degree of interference of thimerosal in the protein formulations and development of a method to nullify the interference by removing thimerosal.
Chattaraj
S C
SC
College of Pharmacy, Nova Southeastern University, Ft. Lauderdale, FL 33328, USA.
Das
S K
SK
D004340:Drug Contamination; D007252:Influenza Vaccines / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D011804:Quinolines / Q000737:chemistry*; D013849:Thimerosal / Q000737:chemistry*
D007252:Influenza Vaccines; D011506:Proteins; D011804:Quinolines; D013849:Thimerosal; C047117:bicinchoninic acid