Glutathione S-transferase-pi gene expression and platinum drug exposure in human lung cancer.
We examined the association between the gene expression levels of glutathione S-transferase-pi (GST-pi) and platinum drug exposure in human lung cancer. First we monitored GST-pi gene expression levels in two lung cancer cell lines and in peripheral mononuclear cells of ten previously untreated lung cancer patients after platinum drug exposure. Next we examined GST-pi gene expression levels in 40 lung cancer autopsy specimens. The GST-pi gene expression levels were assessed by the quantitative reverse transcription-polymerase chain reaction or Northern blot analysis. The GST-pi gene expression was not induced by platinum drugs either in vitro and in vivo within 24 h of exposure. In contrast, GST-pi gene expression levels in lung cancer tissues of patients who had been exposed to platinum drugs at least 1 month before death were significantly higher than that in those of patients who had not been exposed. These results suggest that GST-pi gene expression is associated with chronic exposure to platinum drugs in lung cancer and/or the stress response to xenobiotics.
Oguri
T
T
Second Department of Internal Medicine, Hiroshima University Faculty of Medicine, Kasumi 1-2-3, Minami-ku, 734-8551, Hiroshima, Japan. oguri@mcai.med.hiroshoma-u.ac.jp
Fujiwara
Y
Y
Katoh
O
O
Daga
H
H
Ishikawa
N
N
Fujitaka
K
K
Yamasaki
M
M
Yokozaki
M
M
Isobe
T
T
Ishioka
S
S
Yamakido
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000502:physiology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D009944:Organoplatinum Compounds / Q000378:metabolism / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000502:physiology; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D007527:Isoenzymes; D009944:Organoplatinum Compounds; D018808:Transcription Factor AP-1; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase