title: Repression of Acinetobacter vanillate demethylase synthesis by VanR, a member of the GntR family of transcriptional regulators.
abstract: Vanillate is converted to protocatechuate by the action of vanillate demethylase encoded by vanAB. Convergent upon and overlapping Acinetobacter vanB is an open reading frame encoding a member of the gntR repressor family and designated vanR. This gene organization differs from that found in a Pseudomonas isolate. An Acinetobacter strain with a knockout mutation in vanR constitutively converted vanillate to protocatechuate. Reverse transcriptase-polymerase chain reaction was used to demonstrate that control of vanAB was exerted at the level of transcription.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA.
authors.forename: B
authors.initials: B
authors.lastname: Morawski
authors.forename: A
authors.initials: A
authors.lastname: Segura
authors.forename: L N
authors.initials: LN
authors.lastname: Ornston
mesh_terms: D000150:Acinetobacter / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins; D015536:Down-Regulation; D010090:Oxidoreductases, O-Demethylating / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors; C073902:VanR protein, bacteria; D010090:Oxidoreductases, O-Demethylating; C023284:vanillate O-demethylase