Microemulsion electrokinetic chromatography in suppressed electroosmotic flow environment. Separation of fat-soluble vitamins.
Microemulsion electrokinetic chromatography (MEEKC) was carried out in a pH 2.5 phosphate buffer to effectively suppress the electroosmotic flow (EOF). With 66.6% (w/w) 25 mM phosphate buffer pH 2.5, 20.0% (w/w) 2-propanol, 6.6% (w/w) 1-butanol, 6.0% (w/w) sodium lauryl sulphate (SDS), and 0.8% (w/w) n-octane as the separation medium, the fat-soluble vitamins A palmitate, E acetate, and D3 were baseline separated within 11 min. With strongly suppressed EOF, the polarity of the separation voltage was reversed (positive electrode at the outlet); the n-octane micro droplets surrounded by negatively charged SDS molecules migrated towards the detector. The aqueous part of the microemulsion was modified with 20% (w/w) 2-propanol to improve partition between the n-octane phase and the surrounding aqueous medium. The fat-soluble vitamins were separated in order of decreasing hydrophobicity with a high migration time stability (repeatable within 0.1% RSD). Excellent accuracy and precision were obtained when the system was applied for the determination of vitamin E acetate in commercial vitamin tablets; quantitative data corresponded to 97.0% of label claim, intra-day results varied within 1.72% RSD (n=6), and inter-day results varied within 3.22% RSD (n=5).
Pedersen-Bjergaard
S
S
School of Pharmacy, University of Oslo, Norway.
Naess
O
O
Moestue
S
S
Rasmussen
K E
KE
D002021:Buffers; D002762:Cholecalciferol / Q000302:isolation & purification; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004224:Diterpenes; D004655:Emulsions / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D012015:Reference Standards; D000084562:Retinyl Esters; D012995:Solubility; D024505:Tocopherols; D014801:Vitamin A / Q000031:analogs & derivatives / Q000302:isolation & purification; D014810:Vitamin E / Q000031:analogs & derivatives / Q000302:isolation & purification; D014815:Vitamins / Q000737:chemistry / Q000302:isolation & purification*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D002021:Buffers; D004224:Diterpenes; D004655:Emulsions; D009821:Oils; D000084562:Retinyl Esters; D014815:Vitamins; D014801:Vitamin A; D014810:Vitamin E; D002762:Cholecalciferol; C014794:retinol palmitate; D024502:alpha-Tocopherol; D024505:Tocopherols