A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes.
Diphenyl-1-pyrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O). DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor with tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several kinds of free radicals. It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically.
Okimotoa
Y
Y
Research Center for Advanced Science and Technology, University of Tokyo, Meguro, Japan.
Watanabea
A
A
Nikia
E
E
Yamashitab
T
T
Noguchia
N
N
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010713:Phosphatidylcholines / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000378:metabolism
D005456:Fluorescent Dyes; D005609:Free Radicals; D008054:Lipid Peroxides; D008081:Liposomes; D009943:Organophosphorus Compounds; D016877:Oxidants; D010713:Phosphatidylcholines; D011721:Pyrenes; D012996:Solutions; C066322:diphenyl-1-pyrenylphosphine; C008464:methyl linoleate hydroperoxide; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate