As2O3 induces apoptosis of the human B lymphoma cell line MBC-1.
OBJECTIVE
To see how arsenic trioxide (As2O3) affects proliferation of the human B lymphoma cell line MBC-1.


METHODS
We studied the effect of As2O3 on MBC-1 cells and its mechanism by morphological observation, flow cytometry assay and DNA gel electrophoresis.


RESULTS
As2O3 could upregulate p53 gene expression at protein level, inducing cell apoptosis and inhibiting the proliferation of MBC-1 cells. Upregulation of p53 expression appears to be important in the apoptosis of MBC-1 cells.


CONCLUSIONS
As2O3 can inhibit the proliferation of MBC-1 cells by upregulating p53 gene expression, thus inducing apoptosis.
Shen
L
L
Department of Oncology, Shanghai Institute for Pediatric Research, China.
Chen
T X
TX
Wang
Y P
YP
Lin
Z
Z
Zhao
H J
HJ
Zu
Y Z
YZ
Wu
G
G
Ying
D M
DM
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D002051:Burkitt Lymphoma / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage; D053938:DNA Fragmentation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019254:Genes, bcl-2; D016158:Genes, p53; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D010087:Oxides / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D000970:Antineoplastic Agents; D001152:Arsenicals; D009363:Neoplasm Proteins; D010087:Oxides; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D000077237:Arsenic Trioxide