title: Effect of supplemental methionine, choline and their combinations on the performance and immune response of broilers.
abstract: 1. A study was conducted with broilers to find out the effect of addition of methionine (0.0, 1.5, 3.0 and 4.5 g/kg) and choline (0.0, 1000 and 2000 mg/kg) to a basal diet containing crude protein, 221 g/kg, ME, 12.25 MJ/kg, methionine, 3.6 g/kg and choline, 1300 mg/kg. 2. Supplemental levels of methionine and choline were found to be ineffective in improving the growth, food consumption and food conversion efficiency of broilers. 3. However, a higher value for leucocyte migration inhibition was observed in chicks fed on the diet containing 6.5 g/kg methionine and 1300 mg/kg choline, indicating a significantly improved cellular immune response. 4. HI test and ELISA indicated enhanced antibody titres in chicks receiving 3.0 g/kg methionine and 3300 mg/kg choline, showing a significantly better humoral immune response. 5. Methionine and choline should be supplemented at levels higher than the recommended level of BIS for better health and production in chicks.
authors.affiliation: Central Avian Research Institute, Izatnagar, India. icar@goa.goa.nic.in
authors.forename: B K
authors.initials: BK
authors.lastname: Swain
authors.forename: T S
authors.initials: TS
authors.lastname: Johri
mesh_terms: D000821:Animal Feed; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation; D001835:Body Weight; D002464:Cell Migration Inhibition; D002645:Chickens / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D002794:Choline / Q000008:administration & dosage / Q000378:metabolism* / Q000502:physiology; D019587:Dietary Supplements; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007111:Immunity, Cellular; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000378:metabolism* / Q000502:physiology; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control; D009522:Newcastle disease virus / Q000276:immunology; D011897:Random Allocation; D014611:Vaccination / Q000662:veterinary; D015430:Weight Gain
chemical_list: D000914:Antibodies, Viral; D008715:Methionine; D002794:Choline