The production of hemicellulases by aerobic fungi on medium containing residues of banana plant as substrate.
Trichoderma harzianum strains T4 and T6, Acrophialophora nainiana, and Humicola grisea var. thermoidea were screened for their ability to produce carbohydrate-degrading enzyme activities in a medium containing banana plant residue as the carbon source. The best balance of enzyme activities was obtained from cultures of H. grisea var. thermoidea. Xylanase activity from crude extract of A. nainiana had a maximum activity at pH 5.5-7.0 and a temperature range of 50-55 degrees C. It was stable up to 55 degrees C at pH 7.0 for at least 2 h. The fungi were also able to produce xylanase and pectinase activities when grown on extractives as substrate.
Medeiros
R G
RG
Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília, CEP 70910-900, Brasil.
Soffner
M L
ML
Thomé
J A
JA
Cacais
A O
AO
Estelles
R S
RS
Salles
B C
BC
Ferreira
H M
HM
Lucena Neto
S A
SA
Silva
F G
FG
Filho
E X
EX
D003470:Culture Media; D005638:Fruit / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D003470:Culture Media; D006026:Glycoside Hydrolases; C023305:hemicellulase