title: Hepatic colon cancer metastases in mice: dynamic in vivo correlation with hypoechoic rims visible at US.
abstract: OBJECTIVE
  To use videomicroscopy of tumor-bearing livers of live mice to depict tumors directly to determine the exact nature of rims seen on corresponding ultrasonographic (US) scans.
  
  
  METHODS
  Seventy-six hepatic colorectal cancer metastases were studied in exteriorized livers of 18 mice by using intravital microscopy, US, and histologic examination of the same tumors.
  
  
  RESULTS
  Hypoechoic rims correlated with distended sinusoidal spaces in vivo. These spaces surrounded only locally invasive tumors (mean diameter, 0.85 mm) that had obstructed the supplying terminal portal venules. These spaces, containing adherent leukocytes and tumor cells, gave rise to new tumor vasculature. Results of histologic examination of rims (portal inflammation, congested or compressed sinusoids, cell atrophy) correlated with leukocyte endothelial adherence, occluded sinusoids, and new vessel formation in vivo.
  
  
  CONCLUSIONS
  Unlike results from previous studies, dynamic in vivo observations of peritumoral rims demonstrated distended sinusoidal spaces giving rise to new tumor-penetrating vessels. These sinusoids arose around locally invasive tumors and were associated with more advanced intrahepatic disease. These dynamic observations provide a pathophysiologic explanation for previous histologic correlates of peritumoral rims.
authors.affiliation: Department of Radiology, Beth Israel Deaconess Medical Center-West, Boston, MA 02215, USA. jkruskal@caregroup.harvard.edu
authors.forename: J B
authors.initials: JB
authors.lastname: Kruskal
authors.forename: P
authors.initials: P
authors.lastname: Thomas
authors.forename: I
authors.initials: I
authors.lastname: Nasser
authors.forename: O
authors.initials: O
authors.lastname: Cay
authors.forename: R A
authors.initials: RA
authors.lastname: Kane
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D008099:Liver / Q000000981:diagnostic imaging / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging* / Q000473:pathology / Q000556:secondary*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018715:Microscopy, Video; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured; D014463:Ultrasonography