3D EXAFS refinement of the Cu site of azurin sheds light on the nature of structural change at the metal centre in an oxidation-reduction process: an integrated approach combining EXAFS and crystallography.
Three-dimensional information is obtained for the Cu site in azurin at very high resolution by combining high-resolution crystallographic structures and EXAFS data for the oxidized and reduced form of the protein. This combined approach has allowed us to define the subtle structural changes (<0.1 A) which take place at the Cu site during a single-electron redox process.
Cheung
K C
KC
Faculty of Applied Sciences, De Montfort University, Leicester. England.
Strange
R W
RW
Hasnain
S S
SS
D000421:Alcaligenes; D001400:Azurin / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray / Q000379:methods; D004579:Electron Transport; D008024:Ligands; D009569:Nitric Oxide / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010084:Oxidation-Reduction; D013057:Spectrum Analysis / Q000379:methods; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D008024:Ligands; D009573:Nitrites; D001400:Azurin; D009569:Nitric Oxide; D003300:Copper