title: Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine.
abstract: A new Lactobacillus species from pig small intestine has been identified. In an attempt to isolate Lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. A number of isolates were obtained and primary characterization showed that they were Gram-positive, catalase-negative, non-spore-forming, non-motile rods. Growth occurred at 45 degrees C but not at 15 degrees C and the DNA G+C content was 46 mol%. Cell wall analysis together with DNA-DNA hybridization and analysis of the 16S rRNA sequence revealed that the new isolates represent a previously undescribed Lactobacillus species closely related to L. reuteri, Lactobacillus fermentum and Lactobacillus pontis. The name Lactobacillus mucosae is proposed for this species and the type strain is S32T.
authors.forename: S
authors.initials: S
authors.lastname: Roos
authors.forename: F
authors.initials: F
authors.lastname: Karner
authors.forename: L
authors.initials: L
authors.lastname: Axelsson
authors.forename: H
authors.initials: H
authors.lastname: Jonsson
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007413:Intestinal Mucosa / Q000382:microbiology; D007421:Intestine, Small / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009093:Mucus / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S