Hydroxylated metabolites of 2,4-dichlorophenol imply a fenton-type reaction in Gloeophyllum striatum.
While degrading 2,4-dichlorophenol, two strains of Gloeophyllum striatum, a basidiomycetous fungus causing brown rot decay of wood, simultaneously produced 4-chlorocatechol and 3,5-dichlorocatechol. These metabolites were identified by comparing high-performance liquid chromatography retention times and mass spectral data with those of chemically synthesized standards. Under similar conditions, 3-hydroxyphthalic hydrazide was generated from phthalic hydrazide, a reaction assumed to indicate hydroxyl radical formation. Accordingly, during chemical degradation of 2,4-dichlorophenol by Fenton's reagent, identical metabolites were formed. Both activities, the conversion of 2,4-[U-(14)C]dichlorophenol into (14)CO(2) and the generation of 3-hydroxyphthalic hydrazide, were strongly inhibited by the hydroxyl radical scavenger mannitol and in the absence of iron. These results provide new evidence in favor of a Fenton-type degradation mechanism operative in Gloeophyllum.
Schlosser
D
D
UFZ Centre for Environmental Research Leipzig-Halle, D-06120 Halle, Germany. schloss@hdg.ufz.de
Fahr
K
K
Karl
W
W
Wetzstein
H G
HG
D001487:Basidiomycota / Q000254:growth & development / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006900:Hydroxylation; D007501:Iron
D002250:Carbon Radioisotopes; D002733:Chlorophenols; C045076:Fenton's reagent; D005296:Ferrous Compounds; D002245:Carbon Dioxide; D006861:Hydrogen Peroxide; D007501:Iron; C004762:2,4-dichlorophenol