Preparation and characterization of calixarene-coated capillaries for capillary electrophoresis.
Preparation and characterization of calixarene-coated capillaries for capillary electrophoresis (CE) were exemplified with p-allylcalix[4]arene (pACX4) which was immobilized to the fused silica surface using gamma-methacryloxypropyl-trimethoxysilane (gamma-MAPS) as linking agent. Successful gamma-ACX4 coating was suggested by the greatly decreased electroosmotic flow (EOF), due to the introduction of phenolic hydroxyl groups on the inner surface of the capillaries. A slight slope of EOF versus pH at pH <8 would help make the separation reproducible. The coated columns also featured a low ultraviolet (UV) absorption background and long lifetime (> 6 months at 4<pH<9). Moreover, their special selectivity on phenolic compounds indicated a certain extent of selective interactions between solutes and the calixarene coating, which effected separations partially based on an electrochromatographic mechanism.
Wang
Z
Z
Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences, Beijing.
Chen
Y
Y
Yuan
H
H
Huang
Z
Z
Liu
G
G
D047250:Calixarenes; D019075:Electrophoresis, Capillary / Q000379:methods*; D046911:Macromolecular Substances; D015394:Molecular Structure; D018377:Neurotransmitter Agents / Q000302:isolation & purification; D010636:Phenols; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D046911:Macromolecular Substances; D018377:Neurotransmitter Agents; D010636:Phenols; D047250:Calixarenes