Analysis of the long-term efficacy and selectivity of immunoadsorption columns for low density lipoprotein apheresis.
Immunoadsorption low density lipoprotein (LDL) apheresis is performed with reusable columns containing anti-apolipoprotein B(ApoB) antibodies. We analyzed their long-term efficacy and selectivity. Performance over 60 treatment sessions of six pairs of immunoadsorption LDL apheresis columns was evaluated by analysis of variance using the removal of total cholesterol and ApoB to assess efficacy and the ratio of total cholesterol/high density cholesterol removed to assess selectivity. The removal of cholesterol did not vary significantly with treatment number. The mass of ApoB removed increased significantly (p = 0.002), and the mass of ApoB removed per volume unit of processed plasma showed a trend (p = 0.065) toward an increase with treatment number. Both parameters correlated with the serum ApoB concentration before treatment, which also increased significantly (p = 0.0007) with treatment number. No significant variation of selectivity was found. The efficacy of the LDL apheresis immunoadsorption columns did not decrease after 60 treatment sessions. The columns' selectivity also remained unchanged.
Jovin
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IS
Department of Hemostaseology and Transfusion Medicine, Max-Planck-Institut für physiologische und klinische Forschung, Kerckhoff-Klinik, Bad Nauheim, Germany.
Taborski
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U
Müller-Berghaus
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G
D000704:Analysis of Variance; D000906:Antibodies; D001055:Apolipoproteins B / Q000276:immunology / Q000302:isolation & purification; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods*; D002784:Cholesterol / Q000302:isolation & purification; D006801:Humans; D006937:Hypercholesterolemia / Q000628:therapy*; D007163:Immunosorbent Techniques; D008077:Lipoproteins, LDL / Q000276:immunology / Q000302:isolation & purification*
D000906:Antibodies; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL; D002784:Cholesterol