title: Trauma and inflammation modulate lymphocyte localization in vivo: quantitation of tissue entry and retention using indium-111-labeled lymphocytes.
abstract: OBJECTIVE
  Determine the in vivo localization pattern of indium-111-labeled lymphocytes after a standardized extremity injury or standardized laparotomy and after sterile inflammation of the central nervous system.
  
  
  METHODS
  Prospective animal study with concurrent controls.
  
  
  METHODS
  Animal research laboratory.
  
  
  METHODS
  Male Lewis rats weighing 150-175 g.
  
  
  METHODS
  Indium-111-labeled splenic lymphocytes were injected into animals after a standardized hind limb trauma or laparotomy and after induction of sterile central nervous system inflammation.
  
  
  RESULTS
  Lymphoid and non-lymphoid organs were removed at fixed intervals after lymphocyte injection and the proportion of injected lymphocytes/gram of tissue was determined using a quantitative radionuclide calculation. Results from treated animals were compared with results from untreated control animals. Muscle injury caused early localization of lymphocytes to injured hind limbs, liver, and spleen compared with controls, whereas laparotomy decreased lymphocyte localization in the thymus and colon. Encephalitis increased localization to the central nervous system with no effect on other tissues.
  
  
  CONCLUSIONS
  These results identify a sensitive method to track in vivo leukocyte localization and specifically demonstrate that lymphocyte localization is altered in both traumatic and nontraumatic models of inflammation.
authors.affiliation: Department of Anesthesiology, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Yeager
authors.forename: J A
authors.initials: JA
authors.lastname: DeLeo
authors.forename: P J
authors.initials: PJ
authors.lastname: Hoopes
authors.forename: A
authors.initials: A
authors.lastname: Hartov
authors.forename: L
authors.initials: L
authors.lastname: Hildebrandt
authors.forename: W F
authors.initials: WF
authors.lastname: Hickey
mesh_terms: D000818:Animals; D002464:Cell Migration Inhibition; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D004660:Encephalitis / Q000276:immunology; D007205:Indium Radioisotopes; D008297:Male; D009104:Multiple Trauma / Q000000981:diagnostic imaging / Q000276:immunology*; D011877:Radionuclide Imaging; D051381:Rats; D011917:Rats, Inbred Lew; D018746:Systemic Inflammatory Response Syndrome / Q000000981:diagnostic imaging / Q000276:immunology*; D013601:T-Lymphocytes / Q000000981:diagnostic imaging / Q000276:immunology*; D014018:Tissue Distribution
chemical_list: D007205:Indium Radioisotopes