Generation of 7137 non-redundant expressed sequence tags from a legume, Lotus japonicus.
For comprehensive analysis of genes expressed in a model legume, Lotus japonicus, a total of 22,983 5' end expressed sequence tags (ESTs) were accumulated from normalized and size-selected cDNA libraries constructed from young (2 weeks old) plants. The EST sequences were clustered into 7137 non-redundant groups. Similarity search against public non-redundant protein database indicated that 3302 groups showed similarity to genes of known function, 1143 groups to hypothetical genes, and 2692 were novel sequences. Homologues of 5 nodule-specific genes which have been reported in other legume species were contained in the collected ESTs, suggesting that the EST source generated in this study will become a useful tool for identification of genes related to legume-specific biological processes. The sequence data of individual ESTs are available at the web site: http://www.kazusa.or.jp/en/plant/lotus/EST/.
Asamizu
E
E
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
Nakamura
Y
Y
Sato
S
S
Tabata
S
S
D003062:Codon; D020224:Expressed Sequence Tags; D007887:Fabaceae / Q000235:genetics*; D015723:Gene Library; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D018749:RNA, Plant / Q000032:analysis; D016415:Sequence Alignment
D003062:Codon; D018749:RNA, Plant