Sauchinone, a lignan from Saururus chinensis, attenuates CCl4-induced toxicity in primary cultures of rat hepatocytes.
We used primary cultures of rat hepatocytes injured by the hepatotoxin, carbon tetrachloride (CCl4), as a test system to screen for hepatoprotective compounds from natural products. Sauchinone was isolated from the aerial parts of Saururus chinensis (Saururaceae) by this method. At a concentration of 50 microM, sauchinone significantly reduced the release into the culture medium of glutamic pyruvic transaminase from CCl4-damaged cultures of rat hepatocytes. It has been determined that glutathione, superoxide dismutase and glutathione peroxidase all play important roles in the cellular defense against oxidative stress. Sauchinone appeared to protect primary cultured rat hepatocytes exposed to CCl4 from significant drops in the levels of each of these three specific markers, respectively. Sauchinone also seemed to ameliorate lipid peroxidation as demonstrated by a reduction in the production of the oxidized lipid byproduct, malondialdehyde. These results suggest that sauchinone may exert hepatoprotective activity through antioxidant activity.
Sung
S H
SH
College of Pharmacy, Seoul National University, Korea.
Lee
E J
EJ
Cho
J H
JH
Kim
H S
HS
Kim
Y C
YC
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D004149:Dioxoles / Q000494:pharmacology*; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D001578:Benzopyrans; D004149:Dioxoles; D017705:Lignans; D020011:Protective Agents; C410958:sauchinone; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione