Influenza virus NS1 protein counteracts PKR-mediated inhibition of replication.
The availability of an influenza virus NS1 gene knockout virus (delNS1 virus) allowed us to establish the significance of the biological relationship between the influenza virus NS1 protein and double-stranded-RNA-activated protein kinase (PKR) in the life cycle and pathogenicity of influenza virus. Our results show that the lack of functional PKR permits the delNS1 virus to replicate in otherwise nonpermissive hosts, suggesting that the major function of the influenza virus NS1 protein is to counteract or prevent the PKR-mediated antiviral response.
Bergmann
M
M
Department of Surgery, University of Vienna Medical School, Austria.
Garcia-Sastre
A
A
Carnero
E
E
Pehamberger
H
H
Wolff
K
K
Palese
P
P
Muster
T
T
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity*; D007251:Influenza, Human / Q000235:genetics / Q000276:immunology* / Q000821:virology; D007372:Interferons / Q000276:immunology; D008168:Lung / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D050794:STAT1 Transcription Factor; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication; D019892:eIF-2 Kinase / Q000235:genetics / Q000276:immunology*
D004268:DNA-Binding Proteins; C085820:INS1 protein, influenza virus; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; C494080:Stat1 protein, mouse; D015534:Trans-Activators; D017361:Viral Nonstructural Proteins; D007372:Interferons; D019892:eIF-2 Kinase