CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
CD40 ligand (CD40L)/CD40 interactions play a central role in T-cell-dependent B-cell activation as previously shown by in vitro studies, the phenotype of CD40L knockout mice and the defective expression of CD40L in patients who have X-linked immunodeficiency with hyper-IgM. The distribution of CD40 in cells other than of myeloid and lymphoid lineages has suggested additional functions for this receptor/ligand couple. Here we show that CD40L stimulates myelopoiesis with a noticeable effect on megakaryocytopoiesis in cocultures of hematopoietic progenitor cells and bone marrow stromal cells. These results suggest a mechanism by which T-cell or platelet-associated or soluble CD40L may regulate myelopoiesis. (Blood. 2000;95:3758-3764)
Solanilla
A
A
FR 60, Biologie des Greffes, Université de Bordeaux 2; ETS Franche Comté, Besançon, France.
Déchanet
J
J
El Andaloussi
A
A
Dupouy
M
M
Godard
F
F
Chabrol
J
J
Charbord
P
P
Reiffers
J
J
Nurden
A T
AT
Weksler
B
B
Moreau
J F
JF
Ripoche
J
J
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D023201:CD40 Ligand; D019556:COS Cells; D002478:Cells, Cultured; D018920:Coculture Techniques; D003114:Colony-Forming Units Assay; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D019891:Leukopoiesis / Q000187:drug effects / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D018345:Mice, Knockout; D011247:Pregnancy; D013926:Thrombopoietin / Q000096:biosynthesis*; D014471:Umbilical Veins
D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C084881:flt3 ligand protein; D023201:CD40 Ligand; D013926:Thrombopoietin