Bovine serum albumin-hemoglobin fractionation: significance of ultrafiltration system and feed solution characteristics.
This work investigates the fractionation of similar molecular weight proteins bovine serum albumin (69 kD) and bovine hemoglobin (67 kD) by ultrafiltration. Three different membranes, viz. regenerated cellulose, poly(sulfone) and surface modified poly(acrylonitrile), each with a nominal molecular cutoff rating of 100 kD, were examined. The experiments were conducted in dead end, crossflow and vortex flow filtration modes and the separation was studied as a function of feed pH and ionic strength. Under similar system hydrodynamics, the surface modified poly(acrylonitrile) membrane displayed the highest resolution with minimum membrane fouling. The separation could be improved further by operating at low applied pressure (40 kPa) and high mass transfer (> 20 x 10(-6) m/s) in a vortex flow module. Under these conditions, the highest separation factor of 40 was obtained at the pI of hemoglobin.
Shukla
R
R
Agricultural Bioprocess Laboratory, University of Illinois at Urbana-Champaign 61801, USA.
Balakrishnan
M
M
Agarwal
G P
GP
D005374:Filtration; D006454:Hemoglobins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008567:Membranes, Artificial; D008970:Molecular Weight; D009994:Osmolar Concentration; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*
D006454:Hemoglobins; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine