Efficient retrovirus-mediated transfer of the multidrug resistance 1 gene into autologous human long-term repopulating hematopoietic stem cells.
Pre-clinical studies indicate that efficient retrovirus-mediated gene transfer into hematopoietic stem cells and progenitor cells can be achieved by co-localizing retroviral particles and target cells on specific adhesion domains of fibronectin. In this pilot study, we used this technique to transfer the human multidrug resistance 1 gene into stem and progenitor cells of patients with germ cell tumors undergoing autologous transplantation. There was efficient gene transfer into stem and progenitor cells in the presence of recombinant fibronectin fragment CH-296. The infusion of these cells was associated with no harmful effects and led to prompt hematopoietic recovery. There was in vivo vector expression, but it may have been limited by the high rate of aberrant splicing of the multidrug resistance 1 gene in the vector. Gene marking has persisted more than a year at levels higher than previously reported in humans.
Abonour
R
R
Department of Medicine, Howard Hughes Medical Institute, Indiana University School of Medicine, 1044 W. Walnut, Rm. 402, Indianapolis, IN 46202, USA.
Williams
D A
DA
Einhorn
L
L
Hall
K M
KM
Chen
J
J
Coffman
J
J
Traycoff
C M
CM
Bank
A
A
Kato
I
I
Ward
M
M
Williams
S D
SD
Hromas
R
R
Robertson
M J
MJ
Smith
F O
FO
Woo
D
D
Mills
B
B
Srour
E F
EF
Cornetta
K
K
D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34; D005353:Fibronectins / Q000235:genetics*; D005500:Follow-Up Studies; D018014:Gene Transfer Techniques; D019450:Genes, MDR; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D018237:Germinoma / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008875:Middle Aged; D010865:Pilot Projects; D012190:Retroviridae; D013997:Time Factors; D016896:Treatment Outcome
D018952:Antigens, CD34; D005353:Fibronectins