Improved liquid chromatographic method for the analysis of photosynthetic pigments of higher plants.
The paper presents an improved reversed-phase LC method for the separation of the pigments from green leaves. A good separation of carotenoids and of their cis- and trans-isomers was achieved, especially for the separation of trans-lutein, zeaxanthin, cis-lutein, which are usually not well separated. No perfect separation of alpha-carotene, beta-carotene and pheophytin a was possible, but conditions for a perfect coelution of pheophytin a with either beta-carotene or alpha-carotene were established. Simultaneous equations allowing the determination of pheophytin a and alpha-carotene or pheophytin a and beta-carotene are also given.
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Plant Cytophysiology and Phycology Laboratory, University of Lille, Villeneuve d'Ascq, France.
Schoefs
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B
Lemoine
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Y
D002338:Carotenoids / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D014975:Lutein / Q000032:analysis; D010674:Pheophytins / Q000032:analysis; D010788:Photosynthesis; D010860:Pigments, Biological / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene / Q000031:analogs & derivatives / Q000032:analysis
D010674:Pheophytins; D010860:Pigments, Biological; D024341:Xanthophylls; D065146:Zeaxanthins; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene; C061694:pheophytin a; D014975:Lutein