Novel approach for large-scale, biocompatible, and low-cost fractionation of peptides in proteolytic digest of food protein based on the amphoteric nature of peptides.
A large-scale, biocompatible, and low-cost procedure for peptide fractionation based on the amphoteric nature of peptide is developed. A sample cell (120 x 100 x 50 mm) with four joint tubes (17 mm i.d. and 20 mm in length) on the front and back was prepared. On the end of the joint tubes, a nylon screen (100 mesh)-supported agarose gel layer was formed. Five or nine of the sample cells were connected. A tryptic digest of casein (2.0-3.6 L) was applied to the sample cells. At each end of the sample cell apparatus, an additional cell filled with 0.1 M H(3)PO(4) or NaOH was connected and used as anode and cathode compartments, respectively. Reproducible fractionation of peptide could be achieved by collecting fractions with specific pH values when voltage reached a plateau by applying direct current at constant power. Running time necessary for fractionation of peptide was inversely proportional to electric power and directly proportional to sample volume.
Akahoshi
A
A
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
Sato
K
K
Nawa
Y
Y
Nakamura
Y
Y
Ohtsuki
K
K
D002364:Caseins / Q000737:chemistry*; D003365:Costs and Cost Analysis; D005502:Food; D006868:Hydrolysis; D007525:Isoelectric Focusing / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D014357:Trypsin / Q000737:chemistry
D002364:Caseins; D010455:Peptides; D014357:Trypsin