JIL-1, a chromosomal kinase implicated in regulation of chromatin structure, associates with the male specific lethal (MSL) dosage compensation complex.
JIL-1 is a novel chromosomal kinase that is upregulated almost twofold on the male X chromosome in Drosophila. Here we demonstrate that JIL-1 colocalizes and physically interacts with male specific lethal (MSL) dosage compensation complex proteins. Furthermore, ectopic expression of the MSL complex directed by MSL2 in females causes a concomitant upregulation of JIL-1 to the female X that is abolished in msl mutants unable to assemble the complex. Thus, these results strongly indicate JIL-1 associates with the MSL complex and further suggests JIL-1 functions in signal transduction pathways regulating chromatin structure.
Jin
Y
Y
Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA.
Wang
Y
Y
Johansen
J
J
Johansen
K M
KM
D000818:Animals; D002843:Chromatin / Q000201:enzymology*; D004268:DNA-Binding Proteins; D004330:Drosophila; D029721:Drosophila Proteins; D005260:Female; D018628:Gene Dosage; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006657:Histones / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014960:X Chromosome / Q000201:enzymology*
D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; D019476:Insect Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; C082132:msl-1 protein, Drosophila; C094166:msl-2 protein, Drosophila; C093904:msl-3 protein, Drosophila; D011494:Protein Kinases