Study on DNA polymorphism at D1S8 locus in Hebei Han population.
OBJECTIVE
To study the polymorphism at D1S8 locus and to provide basic information for the construction of DNA fingerprint database and the application in forensic medicine.


METHODS
Minisatellite variant repeat-polymerase chain reaction (MVR-PCR) and polyacrylamide gradient gel electrophoresis followed by silver staining were used to detect the variant repeat sequences at D1S8 locus of 240 unrelated individuals in Hebei Han population, and digital codes were obtained.


RESULTS
Each individual obtained about 30 digital codes, in which none of two unrelated individuals had the same code. The probability of identity of 30 digital codes was 3.55x10(-11). The percentages of three repeat units, a-type, t-type and o-type were 54.77%, 42.54% and 2.69% respectively. The heterozygosity (H) was 0.9837. The excluding probability of paternity(EPP) and polymorphism information content(PIC) were 0.9669 and 0.9833 respectively.


CONCLUSIONS
Because of its high polymorphic nature, D1S8 locus is a valuable marker for forensic identification and paternity testing.
Guo
X
X
Department of Forensic Medicine, Hebei Medical University, Shijiazhuang, Hebei, 050017 P. R. China. Guocarol@hotmail.com
Cong
B
B
Gu
Z
Z
Zuo
M
M
Zhang
G
G
Yao
Y
Y
Peng
Y
Y
Wang
J
J
You
H
H
D002874:Chromosome Mapping; D016172:DNA Fingerprinting; D005554:Forensic Medicine; D006801:Humans; D018895:Microsatellite Repeats; D010334:Paternity; D011110:Polymorphism, Genetic