Cloning of MMP-26. A novel matrilysin-like proteinase.
A cDNA encoding a novel human matrix metalloproteinase (MMP), named MMP-26, was cloned from fetal cDNA. The deduced 261-amino-acid sequence is homologous to macrophage metalloelastase (51.8% identity). It includes only the minimal characteristic features of the MMP family: a signal peptide, a prodomain and a catalytic domain. As with MMP-7, this new MMP does not comprise the hemopexin domain, which is believed to be involved in substrate recognition. A study of MMP-26 mRNA steady states levels reveals, among the tissue examined, a specific expression in placenta. MMP-26 mRNA could also be detected in several human cell lines such as HEK 293 kidney cells and HFB1 lymphoma cells. Recombinant MMP-26 was produced in mammalian cells and used to demonstrate a proteolytic activity of the enzyme on gelatin and beta-casein.
de Coignac
A B
AB
Centre d'immunologie Pierre Fabre, St Julien-en-Genevois, France; INSERM U 346, Hôpital Edouard Herriot, Lyon, France.
Elson
G
G
Delneste
Y
Y
Magistrelli
G
G
Jeannin
P
P
Aubry
J P
JP
Berthier
O
O
Schmitt
D
D
Bonnefoy
J Y
JY
Gauchat
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D020134:Catalytic Domain; D002460:Cell Line; D002522:Chlorocebus aethiops; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism; D053505:Matrix Metalloproteinases, Secreted; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010920:Placenta / Q000201:enzymology*; D011257:Pregnancy Proteins / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014162:Transfection
D018076:DNA, Complementary; D011257:Pregnancy Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C410599:MMP26 protein, human; D020782:Matrix Metalloproteinases; D053505:Matrix Metalloproteinases, Secreted