Cloning and characterization of the ddc homolog encoding L-2,4-diaminobutyrate decarboxylase in Enterobacter aerogenes.
L-2,4-diaminobutyrate decarboxylase (DABA DC) catalyzes the formation of 1,3-diaminopropane (DAP) from DABA. In the present study, the ddc gene encoding DABA DC from Enterobacter aerogenes ATCC 13048 was cloned and characterized. Determination of the nucleotide sequence revealed an open reading frame of 1470 bp encoding a 53659-Da protein of 490 amino acids, whose deduced NH2-terminal sequence was identical to that of purified DABA DC from E. aerogenes. The deduced amino acid sequence was highly similar to those of Acinetobacter baumannii and Haemophilus influenzae DABA DCs encoded by the ddc genes. The lysine-307 of the E. aerogenes DABA DC was identified as the pyridoxal 5'-phosphate binding residue by site-directed mutagenesis. Furthermore, PCR analysis revealed the distribution of E. aerogenes ddc homologs in some other species of Enterobacteriaceae. Such a relatively wide occurrence of the ddc homologs implies biological significance of DABA DC and its product DAP.
Yamamoto
S
S
Faculty of Pharmaceutical Sciences, Okayama University, Japan. syamamoto@pharm.okayama-u.ac.jp
Mutoh
N
N
Tsuzuki
D
D
Ikai
H
H
Nakao
H
H
Shinoda
S
S
Narimatsu
S
S
Miyoshi
S I
SI
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002262:Carboxy-Lyases / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004754:Enterobacter / Q000201:enzymology / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009942:Organometallic Compounds; D010455:Peptides; D011732:Pyridoxal Phosphate / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D036341:Intercellular Signaling Peptides and Proteins; D009942:Organometallic Compounds; C074094:PC 1038; D010455:Peptides; D011732:Pyridoxal Phosphate; C101427:2,4-diaminobutyrate decarboxylase; D002262:Carboxy-Lyases; D008239:Lysine