Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus.
High-performance liquid chromatography-electrospray ionization mass spectrometry has been applied to analyze the flavonoids of Huangqi, the roots of Astragalus mongholicus and A. membranaceus. Eight flavonoids were identified as calycosin-7-O-beta-D-glucoside, calycosin-7-O-beta-D-glucoside-6"-O-malonate (2), ononin, (6aR,11aR)-3-hydroxy-9,10-dimethoxypterocarpan-3-O-bet a-D-glucoside, calycosin, (3R)-7,2'-dihydroxy-3',4'-dimethoxyisoflavan-7-O-beta-D-glucoside, formononetin-7-O-beta-D-glucoside-6"-O-malonate and formononetin by direct comparison with the isolated standards from Huangqi. The existence of (6aR,11aR)-3-hydroxy-9,10-dimethoxypterocarpan, (3R)-7,2'-dihydroxy-3',4'-dimethoxyisoflavan, astrapterocarpanglucoside-6'-O-malonate and astraisoflavanglucoside-6'-O-malonate was detected. This is the first report of flavonoid glycoside malonates in these two Astragalus species, and malonate 2 is a structurally completely identified new compound.
Lin
L Z
LZ
Research Laboratory of Natural Products Chemistry, A.M. Todd Botanicals, Eugene, OR 97402, USA. longze.lin@amtbotanicals.com
He
X G
XG
Lindenmaier
M
M
Nolan
G
G
Yang
J
J
Cleary
M
M
Qiu
S X
SX
Cordell
G A
GA
D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D019684:Magnoliopsida / Q000737:chemistry*; D008314:Malonates / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D018517:Plant Roots / Q000737:chemistry; D012015:Reference Standards; D019685:Rosales / Q000737:chemistry*
D005419:Flavonoids; D008314:Malonates