Subcellular ontogeny of brain pyroglutamyl peptidase I.
The present work studies pyroglutamyl-peptidase I activity in several subcellular fractions of the developing brain. In the synaptosomal fraction, soluble pGlu-peptidase I activity is low until postnatal Day 9 (with a peak at postnatal Day 2) and the activity increases at postnatal Day 15. In later stages there are not significant changes of synaptosomal pGlu-peptidase I activity. However, in the cytosolic fraction the activity is high from ED22 until postnatal Day 9, and afterwards decreases. The changes in the particulate fractions are generally less drastic.
De Gandarias
J M
JM
Department of Physiology, Medical School, University of the Basque Country, Leioa, P.O. Box 699, Bilbao, Spain.
Irazusta
J
J
Gil
J
J
Varona
A
A
Ortega
F
F
Casis
L
L
D015081:2-Naphthylamine / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers; D001921:Brain / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D004622:Embryo, Mammalian; D008297:Male; D011751:Pyroglutamyl-Peptidase I / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure
D015415:Biomarkers; D015081:2-Naphthylamine; D011751:Pyroglutamyl-Peptidase I