title: Persistence of a Salmonella enterica serotype typhimurium clone in Danish pig production units and farmhouse environment studied by pulsed field gel electrophoresis (PFGE)
abstract: The clonal relationship among Salmonella enterica serotype Typhimurium isolates from selected pig production units in Denmark was investigated by the pulsed field gel electrophoresis (PFGE) typing method to determine environmental survival and spread of Salmonella in different herds. Thirty-four Typhimurium isolated during 1996-1998 from porcine faeces and environmental samples from three pig farms designated 1, 3 and 5 were characterised by PFGE using two restriction enzymes. Farm 5 supplied piglets to farm 1 and the herds were located close to each other. Results of PFGE analysis showed both intra- and inter-relationships, i.e. identical PFGE patterns among the faecal and environmental isolates from farm 1 and farm 5. All the isolates from farm 3 irrespective of the source showed identical PFGE patterns, but were different from samples from farms 1 and 5. This study indicates spread between farms and survival of a farm-specific clone. Furthermore, identical PFGE patterns of isolates from piglet supplier and finisher herds indicate that the farrow-to-grower herd of farm 5 was sub-clinically infected prior to delivery to farm 1 and thereby caused the transmission of Salmonella.
authors.affiliation: Danish Veterinary Laboratory, Bülowsvej 27, DK-1790 V, Copenhagen, Denmark. ds@ssi.dk
authors.forename: D
authors.initials: D
authors.lastname: Sandvang
authors.forename: L B
authors.initials: LB
authors.lastname: Jensen
authors.forename: D L
authors.initials: DL
authors.lastname: Baggesen
authors.forename: S B
authors.initials: SB
authors.lastname: Baloda
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D001434:Bacteriophage Typing / Q000662:veterinary; D004269:DNA, Bacterial / Q000032:analysis*; D003718:Denmark; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D004783:Environmental Microbiology; D005243:Feces / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012486:Salmonella typhimurium / Q000145:classification / Q000302:isolation & purification*; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial