Tecoma sambucifolia: anti-inflammatory and antinociceptive activities, and 'in vitro' toxicity of extracts of the 'huarumo' of peruvian incas.
Aqueous and alcoholic extracts of pods and flowers of Tecoma sambucifolia H.B.K. (Bignoniaceae) ('huarumo') were analysed to determine their anti-inflammatory activity (carrageenan-induced edema test), antinociceptive activity (acetic acid writhing test) and 'in vitro' toxicity in Chinese hamster ovary cells, human hepatome cells and human larynx epidermal carcinoma cells. The cytotoxic effects of both extracts were evaluated by two endpoint systems: neutral red uptake assay and tetrazolium assay. The results showed that all extracts have anti-inflammatory and antinociceptive activity, but the highest potency is that of the alcoholic extracts. There were significant differences in cytotoxicity between extracts and among the response of cells to them. The highest cytotoxicity was noted with the alcoholic extract, and the human hepatome cell line was the most sensitive, especially to the alcoholic extract of flowers. The aqueous pod extract appeared to have the best pharmaco-toxicological profile, since it provided a significant reduction of both pain and inflammation together with the lowest cytotoxicity.
Alguacil
L F
LF
Departamento de CC Biomédicas, Universidad San Pablo-CEU, Madrid, Spain.
Galán de Mera
A
A
Gómez
J
J
Llinares
F
F
Morales
L
L
Muñoz-Mingarro
M D
MD
Pozuelo
J M
JM
Vicente Orellana
J A
JA
D000818:Animals; D006224:Cricetinae; D008297:Male; D051379:Mice; D051381:Rats; D019342:Acetic Acid; D018712:Analgesics, Non-Narcotic / Q000302:isolation & purification / Q000494:pharmacology; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001522:Behavior, Animal / Q000187:drug effects; D002351:Carrageenan; D016466:CHO Cells; D004354:Drug Screening Assays, Antitumor; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D007199:Indians, South American; D008519:Medicine, Traditional; D010146:Pain / Q000139:chemically induced / Q000517:prevention & control; D010568:Peru; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured; D006801:Humans
D019342:Acetic Acid; D018712:Analgesics, Non-Narcotic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D002351:Carrageenan; D010936:Plant Extracts