title: Reovirus protein sigmaNS binds in multiple copies to single-stranded RNA and shares properties with single-stranded DNA binding proteins.
abstract: Reovirus nonstructural protein sigmaNS interacts with reovirus plus-strand RNAs in infected cells, but little is known about the nature of those interactions or their roles in viral replication. In this study, a recombinant form of sigmaNS was analyzed for in vitro binding to nucleic acids using gel mobility shift assays. Multiple units of sigmaNS bound to single-stranded RNA molecules with positive cooperativity and with each unit covering about 25 nucleotides at saturation. The sigmaNS protein did not bind preferentially to reovirus RNA over nonreovirus RNA in competition experiments but did bind preferentially to single-stranded over double-stranded nucleic acids and with a slight preference for RNA over DNA. In addition, sigmaNS bound to single-stranded RNA to which a 19-base DNA oligonucleotide was hybridized at either end or near the middle. When present in saturative amounts, sigmaNS displaced this oligonucleotide from the partial duplex. The strand displacement activity did not require ATP hydrolysis and was inhibited by MgCl(2), distinguishing it from a classical ATP-dependent helicase. These properties of sigmaNS are similar to those of single-stranded DNA binding proteins that are known to participate in genomic DNA replication, suggesting a related role for sigmaNS in replication of the reovirus RNA genome.
authors.affiliation: Department of Biochemistry and Institute for Molecular Virology, The College of Agricultural and Life Sciences, University of Wisconsin-Madison, 53706, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Gillian
authors.forename: S C
authors.initials: SC
authors.lastname: Schmechel
authors.forename: J
authors.initials: J
authors.lastname: Livny
authors.forename: L A
authors.initials: LA
authors.lastname: Schiff
authors.forename: M L
authors.initials: ML
authors.lastname: Nibert
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D036022:Capsid Proteins; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018165:Orthoreovirus; D012330:RNA, Double-Stranded / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C053179:sigma protein 3, Reovirus