Single-motor mechanics and models of the myosin motor.
Recent progress in single-molecule detection techniques is remarkable. These techniques have allowed the accurate determination of myosin-head-induced displacements and how mechanical cycles are coupled to ATP hydrolysis, by measuring individual mechanical events and chemical events of actomyosin directly at the single-molecule level. Here we review our recent work in which we have made detailed measurements of myosin step size and mechanochemical coupling, and propose a model of the myosin motor.
Yanagida
T
T
Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Mino, Osaka. yanagida@physl.med.osaka-u.ac.jp
Esaki
S
S
Iwane
A H
AH
Inoue
Y
Y
Ishijima
A
A
Kitamura
K
K
Tanaka
H
H
Tokunaga
M
M
D000199:Actins / Q000737:chemistry / Q000502:physiology; D000205:Actomyosin / Q000737:chemistry / Q000502:physiology; D000818:Animals; D001696:Biomechanical Phenomena; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000502:physiology*; D009119:Muscle Contraction / Q000502:physiology*; D009218:Myosins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*
D000199:Actins; D020409:Molecular Motor Proteins; D000205:Actomyosin; D009218:Myosins