title: TransgenICSI reviewed: foreign DNA transmission by intracytoplasmic sperm injection in rhesus monkey.
abstract: This brief review considers the status of transgenesis by intracytoplasmic sperm injection (ICSI) with nonhuman primates. GFP expressing rhesus macaques embryos (mean = 34.6%; N = 81) were produced by ICSI using rhodamine-tagged DNA encoding the green fluorescence protein (GFP) gene bound on sperm. Rhodamine signal was lost at the egg surface during in vitro fertilization (IVF) but could be traced by dynamic imaging during ICSI within the egg cytoplasm. GFP gene was expressed as early as the 4-cell stage in ICSI embryos but not in embryos produced by in vitro fertilization (IVF). The percentage of GFP expressing blastomeres increased during embryogenesis to the blastocyst stage. Three offspring resulted from seven embryo transfers-a set of anatomically normal twins (a male and a female) stillborn 35 days premature, and a healthy male born at term. Although transgene was not detected in the offspring, the successful production of live primates using DNA bound sperm by ICSI suggests an alternative route to creating transgenic animals. It also raises concern regarding transmission of infectious material during ICSI.
authors.affiliation: Oregon Regional Primate Research Center, and Department of Cell-Developmental Biology, Oregon Health Sciences University, Beaverton 97006, USA.
authors.forename: A W
authors.initials: AW
authors.lastname: Chan
authors.forename: C M
authors.initials: CM
authors.lastname: Luetjens
authors.forename: T
authors.initials: T
authors.lastname: Dominko
authors.forename: J
authors.initials: J
authors.lastname: Ramalho-Santos
authors.forename: C R
authors.initials: CR
authors.lastname: Simerly
authors.forename: L
authors.initials: L
authors.lastname: Hewitson
authors.forename: G
authors.initials: G
authors.lastname: Schatten
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004624:Embryo Transfer; D005260:Female; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008253:Macaca mulatta; D008297:Male; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D004247:DNA