Ginsenosides inhibit capsaicin-activated channel in rat sensory neurons.
Ginsenosides isolated from ginseng are biologically active components. In this study, whole-cell and inside-out configurations of patch clamp technique had been used to test the effect of ginsenosides on the capsaicin-activated channels in cultured small diameter sensory neurons of young rat. Ginsenosides (100 microg/ml) decreased the amplitude of capsaicin-activated currents by 78.2% in whole cell mode. Similarly, ginsenosides decreased capsaicin-activated single-channel activities in a dose-dependent manner in inside-out patches. These results indicate that ginsenosides might directly block capsaicin-activated channels, resulting in attenuation of the currents in rat sensory neurons.
Hahn
J
J
Department of Physiology, Chung-Ang University School of Medicine, 156-756, Seoul, South Korea.
Nah
S Y
SY
Nah
J J
JJ
Uhm
D Y
DY
Chung
S
S
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D002491:Central Nervous System Agents / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D036145:Ginsenosides; D009475:Neurons, Afferent / Q000187:drug effects* / Q000378:metabolism; D009619:Nociceptors / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010146:Pain / Q000188:drug therapy / Q000503:physiopathology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology*
D015220:Calcium Channels; D002491:Central Nervous System Agents; D036145:Ginsenosides; D011955:Receptors, Drug; D012503:Saponins