Isolation and characterization of a novel gene encoding a putative seven-span transmembrane protein, TM7SF3.
As part of a project involving large-scale sequencing of clones randomly selected from a human cDNA library, we isolated a novel human gene, termed TM7SF3 (transmembrane 7 superfamily member 3). Its open reading frame encodes a 570 amino acid protein containing seven putative transmembrane domains. The transcript of this gene was expressed in all human tissues examined, but most abundantlyin kidney. Immunocytochemical analysis demonstrated subcellular localization of TM7SF3 protein at the plasma membrane. We determined the chromosome location of TM7SF3 as 12q11.2-->q12 by a combination of fluorescence in situ hybridization and radiation hybrid mapping.
Akashi
H
H
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Japan.
Han
H J
HJ
Iizaka
M
M
Nakajima
Y
Y
Furukawa
Y
Y
Sugano
S
S
Imai
K
K
Nakamura
Y
Y
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002462:Cell Membrane / Q000737:chemistry; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D005819:Genetic Markers / Q000235:genetics; D006031:Glycosylation; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D007668:Kidney / Q000378:metabolism; D008126:Lod Score; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry; D014162:Transfection; D014407:Tumor Cells, Cultured
D005819:Genetic Markers; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C409878:TM7SF3 protein, human