title: Protection of cattle against bovine leukemia virus (BLV) infection could be attained by DNA vaccination.
abstract: The bovine leukemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into a vehicle expression vector under the human cytomegalovirus (CMV) intermediate early promoter. The intramuscular injection of this plasmid vector generated a cellular immune response. Seven out of ten cows vaccinated with the DNA construct resisted a drastic challenge (500 BLV-infected lymphocytes as an infectious dose).
authors.affiliation: Technical University of Gdańsk, Department of Microbiology, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Brillowska
authors.forename: S
authors.initials: S
authors.lastname: Dabrowski
authors.forename: J
authors.initials: J
authors.lastname: Rułka
authors.forename: P
authors.initials: P
authors.lastname: Kubiś
authors.forename: E
authors.initials: E
authors.lastname: Buzała
authors.forename: J
authors.initials: J
authors.lastname: Kur
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000276:immunology / Q000517:prevention & control*; D015752:Genes, env; D006801:Humans; D007111:Immunity, Cellular; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000494:pharmacology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000914:Antibodies, Viral; D017931:DNA Primers; D019444:Vaccines, DNA; D014765:Viral Vaccines