Determination of clozapine by capillary zone electrophoresis following end-column amperometric detection with simplified capillary/electrode alignment.
Capillary zone electrophoresis was employed for the determination of clozapine using an end-column amperometric detection at a carbon fiber array microdisk electrode with simplified capillary/electrode alignment. The optimum conditions of separation and detection are: Britton-Robinson buffer, pH 2.0 (1.3 x 10(-2) mol/L total concentration of acids, 3.2 x 10(-3) mol/L NaOH), 15 kV for separation voltage, 5 kV and 10 s for injection voltage and injection time, respectively. The limit of detection is 4.2 x 10(-7) mol/L or 1.2 fmole (signal to noise, S/N = 2). The relative standard deviation is 1.4% for the migration time and 2.5% for the electrophoretic peak current. The method was applied to the determination of clozapine in human blood. The recovery of the method is between 94-104%.
Jin
W
W
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China. wenrujin@jn-public.sd.cninfo.net
Xu
Q
Q
Li
W
W
D003024:Clozapine / Q000097:blood*; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results
D003024:Clozapine