Microbiological high throughput screening: an opportunity for the lead discovery process.
Microbial HTS has been implemented at Rhône-Poulenc Rorer through the development of a dedicated robotic platform. This robot (Turbo) has been designed with the aim of fully integrating microbial HTS into the lead discovery processes. Innovative solutions have been found to reach high throughput as well as flexibility. This opens up new prospects for solid-phase microbial screening, taking advantage of the easy implementation and the very low costs of such screens. The different types of microbial screens done in our laboratory, as well as the throughputs and outputs obtained, are described. Some of the specific aspects of microbial HTS, as compared to biochemical and cell-based assays, are also discussed.
Beydon
M H
MH
HTS, Aventis Pharma, Centre de Recherche de Vitry-Alfortville, Vitry sur Seine, France. jerome.becquart@aventis.com
Fournier
A
A
Drugeault
L
L
Becquart
J
J
D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000379:methods*; D005069:Evaluation Studies as Topic; D017930:Genes, Reporter; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000379:methods*; D012371:Robotics; D020798:Two-Hybrid System Techniques