Printing patterns of biospecifically-adsorbed protein.
The advancement of elastomeric patterning techniques in recent years has significantly enhanced our ability to spatially control biomaterial surface chemistry at the micrometre level. The application of this technology to the patterning of biomolecules onto solid surfaces has created many potential applications including the development of advanced biosensors, combinatorial library screening and the formation of tissue engineering templates. In this paper, we describe the direct patterning of protein by microcontact printing. An important consideration for the fabrication of protein micropatterns intended for these applications is the nature of the protein immobilization to a substrate. To date, the patterning of proteins by direct microcontact printing (microCP) has relied on the non-covalent adsorption to a substrate. Ideally, the proteins need to be firmly anchored onto a surface without adversely effecting their activity. Here, the high affinity avidin-biotin receptor-ligand interaction has been exploited to form arrays of avidin molecules onto a polymeric substrate expressing biotin moieties. This has created a generic technique by which any biotinylated species can be subsequently immobilized into defined patterns. Utilizing atomic force microscopy (AFM), the patterned surfaces have been characterized to molecular resolution. The micropatterned sample supported cell adhesion when biotin-(G)11-GRGDS was bound to the avidin bearing arrays.
Patel
N
N
Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, UK. nikin@molprofiles.co.uk
Bhandari
R
R
Shakesheff
K M
KM
Cannizzaro
S M
SM
Davies
M C
MC
Langer
R
R
Roberts
C J
CJ
Tendler
S J
SJ
Williams
P M
PM
D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D001360:Avidin; D001672:Biocompatible Materials / Q000737:chemistry*; D001710:Biotin; D002448:Cell Adhesion; D020724:Elastomers; D005456:Fluorescent Dyes; D008422:Materials Testing; D051379:Mice; D018625:Microscopy, Atomic Force; D011506:Proteins / Q000737:chemistry*; D012235:Rhodamines; D013499:Surface Properties
D001672:Biocompatible Materials; D020724:Elastomers; D005456:Fluorescent Dyes; D011506:Proteins; D012235:Rhodamines; D001360:Avidin; D001710:Biotin