title: Glutathione S-transferase-pi gene expression and platinum drug exposure in human lung cancer.
abstract: We examined the association between the gene expression levels of glutathione S-transferase-pi (GST-pi) and platinum drug exposure in human lung cancer. First we monitored GST-pi gene expression levels in two lung cancer cell lines and in peripheral mononuclear cells of ten previously untreated lung cancer patients after platinum drug exposure. Next we examined GST-pi gene expression levels in 40 lung cancer autopsy specimens. The GST-pi gene expression levels were assessed by the quantitative reverse transcription-polymerase chain reaction or Northern blot analysis. The GST-pi gene expression was not induced by platinum drugs either in vitro and in vivo within 24 h of exposure. In contrast, GST-pi gene expression levels in lung cancer tissues of patients who had been exposed to platinum drugs at least 1 month before death were significantly higher than that in those of patients who had not been exposed. These results suggest that GST-pi gene expression is associated with chronic exposure to platinum drugs in lung cancer and/or the stress response to xenobiotics.
authors.affiliation: Second Department of Internal Medicine, Hiroshima University Faculty of Medicine, Kasumi 1-2-3, Minami-ku, 734-8551, Hiroshima, Japan. oguri@mcai.med.hiroshoma-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Oguri
authors.forename: Y
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: O
authors.initials: O
authors.lastname: Katoh
authors.forename: H
authors.initials: H
authors.lastname: Daga
authors.forename: N
authors.initials: N
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Fujitaka
authors.forename: M
authors.initials: M
authors.lastname: Yamasaki
authors.forename: M
authors.initials: M
authors.lastname: Yokozaki
authors.forename: T
authors.initials: T
authors.lastname: Isobe
authors.forename: S
authors.initials: S
authors.lastname: Ishioka
authors.forename: M
authors.initials: M
authors.lastname: Yamakido
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000502:physiology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics; D009944:Organoplatinum Compounds / Q000378:metabolism / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D007527:Isoenzymes; D009944:Organoplatinum Compounds; D018808:Transcription Factor AP-1; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase