title: Platelet function analysis with PFA-100 in patients medicated with acetylsalicylic acid strongly depends on concentration of sodium citrate used for anticoagulation of blood sample.
abstract: Our data from 214 patients after cessation of long-term therapy with 100 mg/d ASA demonstrate that the determination of platelet-related primary hemostasis in citrated whole blood with PFA-100 is a reliable and sensitive method for the detection of ASA-induced platelet dysfunction. However, the sensitivity of the method is strongly dependent on concentration of sodium citrate used as anticoagulant. The results of PFA-100 testing show a clearly enhanced sensitivity for ASA when blood samples were collected with 0.129 M rather than 0.106 M sodium citrate. According to sample stability, PFA-100 results can only be confirmed up to 1 hour postcollection when blood was anticoagulated with 0.129 M but not with 0.106 M sodium citrate. Therefore, we recommend that testing with PFA-100 in patients with suspected ASA-induced platelet dysfunction should be performed exclusively in blood collected in buffered 0.129 M sodium citrate.
authors.affiliation: Institute for Laboratory Medicine, Hospital Fulda, Fulda, Germany.
authors.forename: K W
authors.initials: KW
authors.lastname: von Pape
authors.forename: E
authors.initials: E
authors.lastname: Aland
authors.forename: J
authors.initials: J
authors.lastname: Bohner
mesh_terms: D016477:Artifacts; D001241:Aspirin / Q000627:therapeutic use*; D001778:Blood Coagulation Disorders / Q000097:blood / Q000139:chemically induced / Q000175:diagnosis; D001800:Blood Specimen Collection; D002951:Citrates / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D006801:Humans; D009994:Osmolar Concentration; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000627:therapeutic use*; D010979:Platelet Function Tests / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000077559:Sodium Citrate
chemical_list: D002951:Citrates; D010975:Platelet Aggregation Inhibitors; D000077559:Sodium Citrate; D001241:Aspirin