Cellular in vivo assay of calpain activity using a fluorescent substrate. Application to study of anoxic liver injury.
Rosser
B G
BG
Liver Disease Unit, University of Manitoba, Winnipeg, Canada.
Gores
G J
GJ
D000818:Animals; D020013:Calcium Signaling; D002135:Calcium-Binding Proteins / Q000235:genetics; D002154:Calpain / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology; D002478:Cells, Cultured; D003374:Coumarins; D015853:Cysteine Proteinase Inhibitors / Q000235:genetics; D004151:Dipeptides; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D005978:Glutathione / Q000378:metabolism; D000860:Hypoxia / Q000201:enzymology*; D017404:In Situ Hybridization, Fluorescence; D008099:Liver / Q000201:enzymology* / Q000293:injuries; D009842:Oligopeptides; D051381:Rats; D013379:Substrate Specificity
D002135:Calcium-Binding Proteins; D003374:Coumarins; D015853:Cysteine Proteinase Inhibitors; D004151:Dipeptides; D005456:Fluorescent Dyes; C114703:N-succinyl-leucyl-leucyl-valyl-tyrosyl-7-amino-4-methylcoumarin; D009842:Oligopeptides; C049925:fura-2-am; C033668:calpastatin; D002154:Calpain; D005978:Glutathione; D016257:Fura-2