title: Deencryption of cellular tissue factor is independent of its cytoplasmic domain.
abstract: Tissue factor (TF) is a transmembrane molecule that, when exposed to plasma, is the key initiator of coagulation. Cellular TF activity is normally "encrypted", but treating cells with calcium ionophore (i.e. , ionomycin or A23187) increases ("deencrypts") TF activity without increasing TF mRNA or antigen expression. Deencryption results from both plasma membrane phosphatidylserine (PS)-dependent and -independent mechanisms; however, the nature of the PS-independent component is unclear. Since deencryption has been suggested to result from release of TF dimers on the cell surface, and since TF's cytoplasmic domain binds to actin-binding protein 280 and interacts with the cytoskeleton, we hypothesized that interactions with the cytoskeleton, through the cytoplasmic domain, play a role in mediating encryption/deencryption. We examined TF deencryption and the role of the cytoplasmic domain in the PS-independent component using baby hamster kidney (BHK) cells expressing full length TF (BHK-TF) or TF lacking its cytoplasmic domain (BHK-descyt) (Sorensen et al. (1999) J. Biol. Chem. 274, 21349). Both BHK-TF and BHK-descyt cells exhibited a dose-dependent, 1.5- to 10-fold increase in TF activity upon treatment with calcium ionophore, and this increase in activity was only partially blocked by annexin V. These results indicate that deencryption is not restricted to cells which naturally express TF and that the PS-independent component of deencryption is intact on cells transfected with either full length or truncated TF. Our results clearly indicate that deencryption is not dependent on an intact cytoplasmic domain in transfected BHK cells.
authors.affiliation: Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Wolberg
authors.forename: R H
authors.initials: RH
authors.lastname: Kon
authors.forename: D M
authors.initials: DM
authors.lastname: Monroe
authors.forename: M
authors.initials: M
authors.lastname: Ezban
authors.forename: H R
authors.initials: HR
authors.lastname: Roberts
authors.forename: M
authors.initials: M
authors.lastname: Hoffman
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism / Q000494:pharmacology; D000001:Calcimycin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D015942:Factor VIIa / Q000378:metabolism / Q000494:pharmacology; D015951:Factor Xa / Q000378:metabolism; D006801:Humans; D007476:Ionophores / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008563:Membrane Lipids / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion / Q000235:genetics*; D013925:Thromboplastin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D017304:Annexin A5; D007476:Ionophores; D008563:Membrane Lipids; D010718:Phosphatidylserines; D000001:Calcimycin; D013925:Thromboplastin; D015942:Factor VIIa; D015951:Factor Xa