Molecular cloning and characterization of phosphatidylcholine transfer protein-like protein gene expressed in murine haploid germ cells.
We have isolated a cDNA clone specifically expressed in spermiogenesis from a subtracted cDNA library of mouse testis. The cDNA consisted of 1392 nucleotides and had an open reading frame of 873 nucleotides encoding a protein of 291 amino acid residues. Computer-mediated homology search revealed that the nucleotide sequence was unique but the deduced amino acid sequence had similarity to mouse phosphatidylcholine transfer protein (PCTP). We named this newly isolated gene PCTP-like protein. Northern blot analysis revealed a 1.4-kilobase mRNA expressed in the testis, kidney, liver, and intestine with the highest level in the testis. Messenger RNA expression in the testis was detected first on Day 23 in postnatal development and then increased up to adulthood. The protein, having a molecular weight of approximately 40 000, was encoded by the mRNA and was detected at the tail of the elongated spermatids and sperm by immunohistochemical staining.
Yamanaka
M
M
Department of Urology, Osaka University Medical School, Suita City, Osaka 565-0871, Japan.
Koga
M
M
Tanaka
H
H
Nakamura
Y
Y
Ohta
H
H
Yomogida
K
K
Tsuchida
J
J
Iguchi
N
N
Nojima
H
H
Nozaki
M
M
Matsumiya
K
K
Okuyama
A
A
Toshimori
K
K
Nishimune
Y
Y
D000375:Aging; D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D015870:Gene Expression; D006238:Haploidy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger / Q000032:analysis; D017385:Sequence Homology; D013082:Sperm Tail / Q000737:chemistry; D013087:Spermatids / Q000737:chemistry; D013094:Spermatozoa / Q000737:chemistry*; D013737:Testis / Q000737:chemistry
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D018076:DNA, Complementary; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger