title: On the mechanism of action of thiamin enzymes in the presence of bivalent metal ions.
abstract: Results on the interactions between the bivalent metal ions Zn2+, Cd2+, Hg2+, Co2+, Ni2+ and 'active aldehyde' thiamin derivatives are reviewed. The techniques used in these studies include spectroscopic methods, i.e., IR-Raman, UV-Vis, multidimensional and multinuclear NMR in solution and in solid state, and X-ray crystal structure determinations. More recently, potentiometric studies on thiamin pyrophosphate and 2-(alpha-hydroxyethyl)thiamin in combination with NMR and EPR techniques were also undertaken. All these studies lead to useful conclusions on the mechanism of action of thiamin enzymes in the presence of bivalent metal ions.
authors.affiliation: University of Ioannina, Department of Chemistry, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Malandrinos
authors.forename: K
authors.initials: K
authors.lastname: Dodi
authors.forename: M
authors.initials: M
authors.lastname: Louloudi
authors.forename: N
authors.initials: N
authors.lastname: Hadjiliadis
mesh_terms: D002413:Cations, Divalent / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology*; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman; D013831:Thiamine / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism*; D013833:Thiamine Monophosphate / Q000737:chemistry / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000737:chemistry / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D004798:Enzymes; D008670:Metals; D013833:Thiamine Monophosphate; D013835:Thiamine Pyrophosphate; D013831:Thiamine