Internalization and nuclear translocation of IFN-gamma and IFN-gammaR: an ultrastructural approach.
IFN-gamma signalling involves the Jak-STAT pathway. However, several hypothesis have been proposed where the receptor and its ligand itself took an active role within the cell. Using a quantitative immunogold approach, we found that both IFN-gamma and its receptor are rapidly internalized and translocated in the nucleus. We found that cell surface heparan sulfate, which binds IFN-gamma, delayed the nuclear accumulation of IFN-gamma suggesting that these molecules serve as storage depot around the cell for local delivery of the cytokine.
Sadir
R
R
CNRS UMR 5578, Université Claude Bernard-Lyon 1, Villeurbanne, France.
Lortat-Jacob
H
H
Morel
G
G
D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis*; D016253:Microscopy, Immunoelectron; D017471:Receptors, Interferon / Q000032:analysis / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D020298:U937 Cells; D000096743:Interferon gamma Receptor
D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor