title: Influence of lipid peroxidation on the nicotinic acetylcholine receptors in PC12 cells.
abstract: The expression of neuronal nicotinic acetylcholine receptors (nAChRs) influenced by lipid peroxidation has been investigated. The results showed that exposure of PC12 cells to a free radical insult (FeSO(4)) induced concentration-dependent increase in level of MDA (malondialdehyde), and a significant decline in MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] reduction. In cultured cells exposed to FeSO(4), a significant dose-dependent decrease in [(3)H] epibatidine binding sites was detected and a significant reduction in B(max) value was observed in the high affinity epibatidine binding site. These results demonstrate that lipid peroxidation in cellular membranes can induce a reduction in numbers of neuronal nAChRs.
authors.affiliation: Department of Clinical Neuroscience, Occupational Therapy and Elderly Care Research, Division of Molecular Neuropharmacology, Huddinge University Hospital B84, Karolinska Institutet, S-141 86, Huddinge, Sweden. zhizhong@kfcmail.hs.sll.se
authors.forename: Z
authors.initials: Z
authors.lastname: Guan
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: A
authors.initials: A
authors.lastname: Nordberg
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005296:Ferrous Compounds / Q000494:pharmacology; D008054:Lipid Peroxides / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D018722:Nicotinic Agonists / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D016716:PC12 Cells / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D005296:Ferrous Compounds; D008054:Lipid Peroxides; D018722:Nicotinic Agonists; D011725:Pyridines; D011978:Receptors, Nicotinic; D013778:Tetrazolium Salts; D013844:Thiazoles; C020748:ferrous sulfate; D008315:Malondialdehyde; C022616:thiazolyl blue; C082748:epibatidine