Accurate multiplex polymerase chain reaction assay for gender determination from a single cell.
For gender determination of preimplantation embryos or circulating fetal cells in maternal blood, we developed a multiplex polymerase chain reaction assay from a single cell. This assay which co-amplifies X (DXZ1)- and Y (DYZ1)-specific repeat sequences, yields a 308-bp band in females and two bands of 154 and 308 bp in males. In a randomized, blinded assay of 100 isolated single amniocytes, 99 (99%) were amplified successfully. All 50 of the XY cells were correctly diagnosed as male (100%), whereas 49 of the 50 XX cells were diagnosed as female (98%). This accurate and efficient assay may be applicable in these clinical settings.
Hashiba
T
T
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
Sueoka
K
K
Kuroshima
M
M
Asada
H
H
Kuji
N
N
Yoshimura
Y
Y
D000653:Amniotic Fluid / Q000166:cytology; D005260:Female; D005333:Fetus / Q000166:cytology*; D006801:Humans; D007621:Karyotyping; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019849:Sex Determination Processes