title: In vivo EPR dosimetry of accidental exposures to radiation: experimental results indicating the feasibility of practical use in human subjects.
abstract: Low frequency electron paramagnetic resonance (EPR) provides the potential advantage of making accurate and sensitive measurements of absorbed radiation dose in teeth in situ, i.e. without removing the teeth from the potential victim. The potential limiting factors for making such measurements are: (1) whether low frequency EPR is sufficiently sensitive to detect radiation-induced signal in human teeth; (2) whether sufficient sensitivity can be maintained under in vivo conditions. In this manuscript, we summarize results indicating that this approach is feasible. Using 1.2 GHz EPR spectroscopy, we found that the lower limit for these measurements in isolated human teeth is 0.2 Gy or lower. Measurements of radiation-induced EPR signals in the teeth of living rats were achieved with sufficient sensitivity to indicate that, when taking into consideration the larger mass of human teeth, similar measurements in human teeth in situ would provide sensitivity in the dose range for potential accidental exposures. We estimate that the current lower limit for detecting radiation doses in human teeth in situ (in vivo) is 0.5-1.0 Gy; this would be sufficient for determining if a person has been exposed to potentially life threatening doses of ionizing radiation. The limiting factor for sensitivity appears to be background signals rather than signal/noise, and there are feasible means to overcome this problem and further increase sensitivity. The additional instrumental developments required to make an effective in vivo EPR dosimetric spectrometer for the measurements in teeth in human subjects in situ, seem quite achievable.
authors.affiliation: EPR Center for the Study of Viable Biological Systems, Dartmouth Medical School, Hanover, NH 03755, USA.
authors.forename: M
authors.initials: M
authors.lastname: Miyake
authors.forename: K J
authors.initials: KJ
authors.lastname: Liu
authors.forename: T M
authors.initials: TM
authors.lastname: Walczak
authors.forename: H M
authors.initials: HM
authors.lastname: Swartz
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008297:Male; D018788:Radioactive Hazard Release; D011874:Radiometry / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D014070:Tooth / Q000528:radiation effects