title: Isolation of a 41-kDa protein with cell adhesion activity for animal tumor cells from the mushroom Hypsizigus marmoreus by affinity chromatography with type IV collagen immobilized on agarose.
abstract: A type IV collagen-binding protein of 41 kDa was isolated from the mushroom Hypsizigus marmoreus and the protein was designated as HM41. The Western blotting analysis with anti-HM41 antibodies demonstrated that HM41 was unrelated to HM23, which had been shown to have an affinity for type IV collagen. The microsequence analysis of the membrane-blotted peptides generated by fragmentation with cyanogen bromide showed no homologous proteins reported. HM41 had cell adhesion-promoting activity for murine Lewis lung carcinoma LL2 cells and human fibrosarcoma HT1080 cells. These results indicate that HM41 is a hitherto undescribed fungus protein that can interact both with animal extracellular matrix protein type IV collagen and with animal tumor cells.
authors.affiliation: School of Food and Nutritional Sciences, University of Shizuoka, Yada, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shoji
authors.forename: M
authors.initials: M
authors.lastname: Isemura
authors.forename: H
authors.initials: H
authors.lastname: Muto
authors.forename: S
authors.initials: S
authors.lastname: Isemura
authors.forename: Y
authors.initials: Y
authors.lastname: Aoyagi
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D000818:Animals; D018827:Carcinoma, Lewis Lung; D002448:Cell Adhesion / Q000187:drug effects; D002846:Chromatography, Affinity / Q000379:methods; D003094:Collagen / Q000378:metabolism*; D005354:Fibrosarcoma; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D016023:Integrins / Q000302:isolation & purification; D051379:Mice; D038981:Receptors, Collagen; D012685:Sepharose; D014407:Tumor Cells, Cultured
chemical_list: D005656:Fungal Proteins; D016023:Integrins; D038981:Receptors, Collagen; D003094:Collagen; D012685:Sepharose