title: Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment.
abstract: Mast cells upon stimulation through high affinity IgE receptors massively release inflammatory mediators by the fusion of specialized secretory granules (related to lysosomes) with the plasma membrane. Using the RBL-2H3 rat mast cell line, we investigated whether granule secretion involves components of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) machinery. Several isoforms of each family of SNARE proteins were expressed. Among those, synaptosome-associated protein of 23 kDa (SNAP23) was central in SNARE complex formation. Within the syntaxin family, syntaxin 4 interacted with SNAP23 and all vesicle-associated membrane proteins (VAMPs) examined, except tetanus neurotoxin insensitive VAMP (TI-VAMP). Overexpression of syntaxin 4, but not of syntaxin 2 nor syntaxin 3, caused inhibition of FcepsilonRI-dependent exocytosis. Four VAMP proteins, i.e., VAMP2, cellubrevin, TI-VAMP, and VAMP8, were present on intracellular membrane structures, with VAMP8 residing mainly on mediator-containing secretory granules. We suggest that syntaxin 4, SNAP23, and VAMP8 may be involved in regulation of mast cell exocytosis. Furthermore, these results are the first demonstration that the nonneuronal VAMP8 isoform, originally localized on early endosomes, is present in a regulated secretory compartment.
authors.affiliation: Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Paumet
authors.forename: J
authors.initials: J
authors.lastname: Le Mao
authors.forename: S
authors.initials: S
authors.lastname: Martin
authors.forename: T
authors.initials: T
authors.lastname: Galli
authors.forename: B
authors.initials: B
authors.lastname: David
authors.forename: U
authors.initials: U
authors.lastname: Blank
authors.forename: M
authors.initials: M
authors.lastname: Roa
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D015550:Cell Degranulation / Q000276:immunology; D003594:Cytoplasmic Granules / Q000378:metabolism*; D005089:Exocytosis / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism* / Q000502:physiology*; D020033:Protein Isoforms / Q000378:metabolism; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; D051381:Rats; D017455:Receptors, IgE / Q000502:physiology; D050600:SNARE Proteins; D012995:Solubility; D013347:Subcellular Fractions / Q000378:metabolism; D013744:Tetanus Toxin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; D017455:Receptors, IgE; C100493:SNAP23 protein, human; D050600:SNARE Proteins; D013744:Tetanus Toxin; C496926:Vamp7 protein, rat; C491830:Vamp8 protein, rat; D033921:Vesicular Transport Proteins