Prevention of monocyte adhesion and inflammatory cytokine production during blood platelet storage: an in vitro model with implications for transfusion practice.
A novel platelet additive solution [ThromboSoltrade mark (TS)] was designed to allow extended refrigerated platelet storage. It has been shown to preserve platelet function and prevent cytokine accumulation in platelet concentrates stored for up to 9 days. It consists of amiloride, adenosine, sodium nitroprusside, dipyridamole, quinacrine, and ticlopidine. We hypothesized that the cytokine inhibition may be due to prevention of monocyte (MC) adhesion and activation on the surfaces of platelet storage bag plastic polymers. In an in vitro model, we incubated purified peripheral blood MCs on discs of polyolefin and polyvinylchloride from platelet storage bags, and on polystyrene, in the presence of TS for up to 7 days. We found that after incubation with TS, adherent MC numbers were decreased by >80-95% compared with controls on all surfaces examined. Levels of cytokines [interleukin (IL)-1beta, IL-1RA, IL-6, IL-8, and tumor necrosis factor-alpha] were low in wells with TS but rose progressively in the controls during incubation. Amiloride alone had similar effects on adhesion and cytokine release as the complete TS preparation. Removing amiloride from TS abrogated these effects. These findings suggest an important role for TS and amiloride in monocyte function, and have implications for the development of agents designed for prolonged platelet storage.
Wood
E M
EM
Blood Bank and Transfusion Medicine Service, University Hospitals of Cleveland, Ohio 44106, USA.
Colton
E
E
Yomtovian
R A
RA
Currie
L M
LM
Connor
J
J
Anderson
J M
JM
D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D002448:Cell Adhesion; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D007249:Inflammation; D009000:Monocytes / Q000502:physiology; D012996:Solutions
D016207:Cytokines; D012996:Solutions