Mechanistic studies on the repair of a novel DNA photolesion: the spore photoproduct.
[formula: see text] UV irradiation of spores results in the formation of the spore photoproduct. This novel DNA photolesion is repaired in the germinating spore in a reaction catalyzed by the spore photoproduct lyase. Model studies, using a simple bispyrimidine, suggest that this repair reaction proceeds by hydrogen abstraction from C6 of the spore photoproduct followed by beta-scission of the bond linking the two pyrimidines and back hydrogen atom transfer.
Mehl
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RA
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Begley
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TP
D002384:Catalysis; D004249:DNA Damage; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism; D011506:Proteins; D013171:Spores, Bacterial / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*
D004269:DNA, Bacterial; D011506:Proteins; C088014:spore photoproduct lyase; D004255:Deoxyribodipyrimidine Photo-Lyase