title: Intranasal immunization with protein-linked phosphorylcholine protects mice against a lethal intranasal challenge with streptococcus pneumoniae.
abstract: Immunization against phosphorylcholine (PC) linked to a protein protects mice against Streptococcus pneumoniae when used parenterally, and against Salmonella typhimurium when used orally after entrapment in D,L-Lactide-co-Glycolide microspheres. Here, we immunized BALB/c mice intranasally with a serotype 3 S. pneumoniae strain. Immunization was followed by a rise in anti-PC IgA and IgG titers in serum and in pulmonary secretions, but not by any rise in anti ds-DNA antibody nor any glomerular Ig deposition. The survival rates were 91 and 76% in the two groups of mice, respectively. These rates were significantly higher than those in control mice immunized intranasally either with Thyr loaded in microspheres (0%), blank microspheres (22%), free Thyr (17%), and saline (18%). This demonstrates that the mucosal route is effective for vaccination against S. pneumoniae pneumonia with PC linked to a protein carrier. It constitutes another important step forward in the development of the concept that PC can be used as a mucosal immunogen for protection against the different diseases caused by PC-bearing bacteria.
authors.affiliation: UMR-CNRS 8612, Faculté de Pharmacie, 92296 Chatenay-Malabry Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Trolle
authors.forename: E
authors.initials: E
authors.lastname: Chachaty
authors.forename: N
authors.initials: N
authors.lastname: Kassis-Chikhani
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: E
authors.initials: E
authors.lastname: Fattal
authors.forename: P
authors.initials: P
authors.lastname: Couvreur
authors.forename: B
authors.initials: B
authors.lastname: Diamond
authors.forename: J
authors.initials: J
authors.lastname: Alonso
authors.forename: A
authors.initials: A
authors.lastname: Andremont
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D005260:Female; D018928:Immunity, Mucosal; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000008:administration & dosage / Q000276:immunology*; D011008:Pneumococcal Infections / Q000401:mortality / Q000517:prevention & control*
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010767:Phosphorylcholine