The role of the sympathetic nervous system in radiation-induced apoptosis in jejunal crypt cells of spontaneously hypertensive rats.
To evaluate the effect of the sympathetic nervous system on radiation-induced apoptosis in jejunal crypt cells, apoptosis levels were compared in spontaneously hypertensive rats (SHR), animals which are a genetic hyperfunction model of the sympathetic nervous system, and normotensive Wistar-Kyoto rats (WKY). SHR and WKY were exposed to whole body X-ray irradiation at doses from 0.5 to 2 Gy. The apoptotic index in jejunal crypt cells was significantly greater in SHR than in WKY at each time point after irradiation and at each dose. WKY and SHR were treated with reserpine to induce sympathetic dysfunction, and were subsequently exposed to irradiation. Reserpine administration to SHR or WKY resulted in a significant suppression of apoptosis. p53 accumulation was detected in the jejunum in both WKY and SHR after irradiation by Western blotting analysis. There were no significant differences in the levels of p53 accumulation in irradiated intestine between WKY and SHR. These findings suggested that hyperfunction of the sympathetic nervous system is involved in the mechanism of high susceptibility to radiation-induced apoptosis of the jejunal crypt cells.
Matsuu
M
M
Department of Molecular Pathology, Nagasaki University School of Medicine, Japan. f1173@cc.nagasaki-u.ac.jp
Shichijo
K
K
Nakamura
Y
Y
Ikeda
Y
Y
Naito
S
S
Ito
M
M
Okaichi
K
K
Sekine
I
I
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D001342:Autonomic Nervous System Diseases / Q000235:genetics / Q000503:physiopathology; D004298:Dopamine / Q000378:metabolism; D006973:Hypertension / Q000209:etiology / Q000235:genetics; D007413:Intestinal Mucosa / Q000473:pathology / Q000528:radiation effects*; D007583:Jejunum / Q000294:innervation / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism; D011833:Radiation Injuries, Experimental / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012110:Reserpine; D013563:Sympathectomy, Chemical; D013564:Sympathetic Nervous System / Q000378:metabolism / Q000503:physiopathology*; D014916:Whole-Body Irradiation
D012110:Reserpine; D004298:Dopamine; D009638:Norepinephrine