Regulation of presynaptic terminal organization by C. elegans RPM-1, a putative guanine nucleotide exchanger with a RING-H2 finger domain.
Presynaptic terminals contain highly organized subcellular structures to facilitate neurotransmitter release. In C. elegans, the typical presynaptic terminal has an electron-dense active zone surrounded by synaptic vesicles. Loss-of-function mutations in the rpm-1 gene result in abnormally structured presynaptic terminals in GABAergic neuromuscular junctions (NMJs), most often manifested as a single presynaptic terminal containing multiple active zones. The RPM-1 protein has an RCC1-like guanine nucleotide exchange factor (GEF) domain and a RING-H2 finger. RPM-1 is most similar to the Drosophila presynaptic protein Highwire (HIW) and the mammalian Myc binding protein Pam. RPM-1 is localized to the presynaptic region independent of synaptic vesicles and functions cell autonomously. The temperature-sensitive period of rpm-1 coincides with the time of synaptogenesis. rpm-1 may regulate the spatial arrangement, or restrict the formation, of presynaptic structures.
Zhen
M
M
Department of Biology, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
Huang
X
X
Bamber
B
B
Jin
Y
Y
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins / Q000235:genetics; D004330:Drosophila / Q000378:metabolism; D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000502:physiology*; D007202:Indicators and Reagents / Q000493:pharmacokinetics; D008164:Luminescent Proteins / Q000493:pharmacokinetics; D006899:Mixed Function Oxygenases; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics; D009474:Neurons / Q000502:physiology; D017729:Presynaptic Terminals / Q000378:metabolism / Q000502:physiology*; D018079:Receptors, GABA / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000502:physiology; D013572:Synaptic Vesicles / Q000378:metabolism / Q000648:ultrastructure; D014018:Tissue Distribution; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D029721:Drosophila Proteins; D020662:Guanine Nucleotide Exchange Factors; C409633:HIW protein, Drosophila; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; C409634:RPM-1 protein, C elegans; D018079:Receptors, GABA; D049452:Green Fluorescent Proteins; D006899:Mixed Function Oxygenases; C114022:MYCBP2 protein, human; D044767:Ubiquitin-Protein Ligases