Analysis of baclofen by capillary electrophoresis with laser-induced fluorescence detection.
A new analytical method for baclofen (4-amino-3-p-chlorophenylbutyric acid) based on capillary electrophoretic separation and laser-induced fluorescence detection has been developed. Naphthalene-2,3-dicarboxaldehyde was used for precolumn derivatization of the non-fluorescent drug. Optimal separation and detection were obtained with an electrophoretic buffer of 50 mM sodium borate (pH 9.5) and a He-Cd laser (excitation at 442 nm, emission at 500 nm). Linearity (r > or = 0.99) over three orders of magnitude was generally obtained and the concentration limit of detection was in the nanomolar level. Coupled with a simple cleanup procedure, the method was successfully applied to the analysis of baclofen in human plasma. Recovery of spiked baclofen in plasma was 98%. The relative standard deviation values on peak size and migration time were 7.9% and 0.4%, respectively. The limit of detection of baclofen in plasma was 10 ng/ml.
Chiang
M T
MT
Department of Chemistry, Tunghai University, Taichung, Taiwan.
Chang
S Y
SY
Whang
C W
CW
D001418:Baclofen / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D018755:GABA Agonists / Q000032:analysis*; D006801:Humans; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000379:methods*
D018755:GABA Agonists; D001418:Baclofen