The combined effect against colon-26 cells of heat treatment and immunization with heat treated colon-26 tumour cell extract.
Cancer vaccines represent a promising new strategy for immunotherapy against cancer, but their effects are insufficiently understood. The effect of heat treatment against mouse colon adenocarcinoma cell line (colon-26), and combined effects of heat treatment and immunizing host animals with heat treated colon-26 cell extracts were investigated. Heat treatment of colon-26 cells induced heat-shock protein 70 (HSP70), but not other HSP. Immunization of BALB/cJ mice with heat treated colon-26 cell extract, which was enriched in HSP70, elicited antitumour immunity against subcutaneously injected colon-26 cells. Furthermore, combination therapy of heat treatment and immunization with heat treated colon-26 cell extract significantly reduced tumour volumes compared with heat treatment alone. Similar immunization enhanced the cytotoxic activity of mouse splenic lymphocytes against untreated and heat treated colon-26 cells in an in vitro assay, as well as against heat treated allogenic mouse lymphoma cell line (YAC-1). These findings suggest possible usefulness of heat treated cancer cell extract as a cancer vaccine, especially if given in combination with hyperthermia.
Okamoto
M
M
Second Department of Surgery, Toyama Medical and Pharmaceutical University, Japan.
Tazawa
K
K
Kawagoshi
T
T
Maeda
M
M
Honda
T
T
Sakamoto
T
T
Tsukada
K
K
D000230:Adenocarcinoma / Q000276:immunology / Q000628:therapy*; D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000302:isolation & purification; D019496:Cancer Vaccines / Q000008:administration & dosage*; D003110:Colonic Neoplasms / Q000276:immunology / Q000628:therapy*; D003131:Combined Modality Therapy; D003602:Cytotoxicity, Immunologic; D018840:HSP70 Heat-Shock Proteins / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D006979:Hyperthermia, Induced / Q000379:methods*; D007114:Immunization; D008223:Lymphoma / Q000276:immunology / Q000628:therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; D018840:HSP70 Heat-Shock Proteins