[Effect of eosin Y on Ca2+-dependent activity of Ca2+-Mg2+-ATPase in smooth muscle cell membrane].
With the aim to elucidate mechanism of eosin Y inhibitory effect on the Ca(2+)-transporting ATPase activity of myometrial cell plasma membrane effect of this inhibitor on the maximal initial rate of ATP hydrolysis reaction, catalyzed by Ca2+, Mg(2+)-ATPase, and on the enzyme affinity for Ca2+ was studied. It was established that eosin Y decreased the rate of Ca2+, Mg(2+)-ATPase catalitic turnover determined by Ca2+ and had no effect on enzyme affinity for this cation.
Chernysh
I H
IH
O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv.
Slincheno
N M
NM
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002384:Catalysis; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology*; D005260:Female; D009130:Muscle, Smooth / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009215:Myometrium / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium; D004801:Eosine Yellowish-(YS)