Relationship among oxygenation, myoelectric activity, and lactic acid accumulation in vastus lateralis muscle during exercise with constant work rate.
The purpose of this study was to determine whether oxygenation in localized working muscle depended on the muscle activity and on the lactic acidosis level. Seven healthy male subjects underwent the five 6-min cycling exercises with work rates of 50 watts (25.0 +/- 5.0% VO2max), 100 watts (36.6 +/- 6.2% VO2max), 150 watts (50.6 +/- 7.7% VO2max), 200 watts (67.8 + 6.9% VO2max), and 250 watts (82.9 +/- 7.5% VO2max) while gas exchange parameters and blood lactate concentration (BL) were measured. We also measured oxygenated hemoglobin and myoglobin concentration (oxy-Hb/Mb) with continuous-wave near infrared spectroscopy (NIR) and surface myoelectric activity with surface electrodes (EMG). The NIR probe and electrodes were positioned on the vastus lateralis muscle of the right leg. The relative change in oxy-Hb/Mb was estimated by regarding oxy-Hb/Mb in the resting condition as 100% and that obtained during thigh occlusion as 0%. The mean values of oxy-Hb/Mb and integrated EMG (iEMG) were determined from 5'30" to 6'00" at each work rate. The percentage of oxy-Hb/Mb was sustained at the first two work rates corresponding to 25.0 +/- 5.0 and 36.6 +/- 6.2% VO2max and decreased slightly at 150 watts corresponding to 50.6 +/- 7.7% VO2max, which was followed by a linear decrease at 200 and 250 watts corresponding to 67.8 +/- 6.9 and 82.9 +/- 7.5% VO2max. The iEMG, however, was increased slowly at 25.0 +/- 5.0 to 50.6 +/- 7.7% VO2max, and a rapid increment of the iEMG occurred at 67.8 +/- 6.9 and 82.9 +/- 7.5% VO2max. BL was sustained at 25.0 +/- 5.0 to 50.6 +/- 7.7% VO2max and increased linearly at 67.8 +/- 6.9 and 82.9 +/- 7.5% VO2max. There was a significant negative correlation for each subject between the percentage of oxy-Hb/Mb and iEMG (r = -0.947 to -0.993), between the percentage of oxy-Hb/Mb and BL (r = -0.890 to -0.982), and between the percentage of oxy-Hb/Mb and VO2 (r = -0.929 to -0.994) These results indicated that oxygenated hemoglobin/myoglobin concentration measured with NIR reflected the muscle activity and the lactic acidosis.
Miura
H
H
Laboratory for Sports Science, Faculty of Integrated Arts and Sciences, University of Tokushima, Japan. hajime-m@ias.tokushima-u.ac.jp
Araki
H
H
Matoba
H
H
Kitagawa
K
K
D000140:Acidosis, Lactic / Q000097:blood / Q000175:diagnosis*; D000328:Adult; D004576:Electromyography; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000502:physiology*; D009211:Myoglobin / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology; D010108:Oxyhemoglobins / Q000378:metabolism; D019265:Spectroscopy, Near-Infrared / Q000379:methods; D013848:Thigh
D009211:Myoglobin; D010108:Oxyhemoglobins; D019344:Lactic Acid