Analysis of papillomavirus consensus L1 gene in a closed colony of baboons (Papio anubis).
OBJECTIVE
The purpose of this study was to analyze cervical specimens and semen from a closed colony of baboons for the presence of the papillomavirus consensus L1 gene.


METHODS
Cervical swabs were collected from lightly anesthetized female baboons. Semen was collected from a male baboon by standard electroejaculation techniques. Deoxyribonucleic acid was extracted from the cells by two different methods and analyzed by polymerase chain reaction targeting the L1 consensus gene common for >25 genital papillomaviruses.


RESULTS
Analyses of the polymerase chain reaction-amplified products did not reveal bands for the papillomavirus in either the cervical specimens or the semen.


CONCLUSIONS
The hypothesis of a linkage between primates with papillomavirus as a common factor is not supported by the results of this study. This information is also important in assisting clinicians in setting up specific pathogen-free colonies of baboons.
Wonderly
D E
DE
Departments of Gynecology and Obstetrics, Physiology and Pharmacology, and Surgery, Loma Linda University School of Medicine, CA 92354, USA.
Chan
P J
PJ
Cseh
S
S
Jacobson
J D
JD
Bailey
L
L
D000818:Animals; D036022:Capsid Proteins; D002584:Cervix Uteri / Q000821:virology; D016384:Consensus Sequence; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005814:Genes, Viral; D008297:Male; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics*; D010215:Papio / Q000821:virology*; D016133:Polymerase Chain Reaction; D012661:Semen / Q000821:virology
D036022:Capsid Proteins; D004279:DNA, Viral; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral