Selective transcriptional regulations in the human liver cell by hepatitis B viral X protein.
The hepatitis B viral X protein (HBx) is known as a transcription factor and potential oncogene. To gain a better view of the effect of HBx on the transcriptional regulation in the human liver cell, we constructed a HepG2 cell line stably expressing HBx (HepG2-HBx), and performed cDNA microarray analysis on 588 cellular cDNAs comparing with untransformed control cells. Two genes (IGFR-2, RhoA) of oncogenes, one gene (p55CDC) of cell cycle regulators, three genes (thrombin receptor, MLK-3, MacMARCKS) of intracellular transducers, one gene (HSP27) of stress response proteins, two genes (FAST kinase, Bak) of apoptosis response proteins, one gene (p21(WAF)) of transcription factors were highly up-regulated; one gene (transcription elongation factor SII) of transcription factors and two genes (monocyte chemotactic protein 1, T-lymphocyte-secreted protein I-309) of growth factors were highly down-regulated. These results showed selective transcriptional regulation by HBx in the human liver cell.
Han
J
J
School of Biological Sciences, Seoul National University, Korea.
Yoo
H Y
HY
Choi
B H
BH
Rho
H M
HM
D017209:Apoptosis / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000235:genetics; D018816:Genes, cdc; D006133:Growth Substances / Q000235:genetics; D006515:Hepatitis B virus; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D009857:Oncogenes / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured; D054334:Viral Regulatory and Accessory Proteins; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D006133:Growth Substances; D012333:RNA, Messenger; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; D051176:beta Catenin; C057628:hepatitis B virus X protein; D018799:Intercellular Adhesion Molecule-1