Involvement of the glutathione S-conjugate compounds and the MRP protein in Tc-99m-tetrofosmin and Tc-99m-sestamibi uptake in glioma cell lines.
The objective of this study was to compare the accumulation of Tc-99m-tetrofosmin and Tc-99m-sestamibi in four grade IV glioma cell lines and to correlate their accumulation with the multidrug resistance of the cells. Tc-99m-tetrofosmin in all glioma cell lines showed slightly higher uptake and more efficient release beyond 150 min than Tc-99m-sestamibi and the retention of both tracers in the cells was to a certain extend inversely proportional to their degree of multidrug resistance. The results obtained showed that the efflux of both tracers was carried out only in part through the MRP/GS-X pump system. Tc-99m-tetrofosmin showed good potential as a marker of recurrent malignant glioma and in vivo studies are currently underway to confirm these observations.
Perek
N
N
Department of Biophysics and Image Treatment, Research Group "Cell Death and Neoplasia," Faculty of Medicine Jacques Lisfranc, Saint Etienne, France.
Prevot
N
N
Koumanov
F
F
Frere
D
D
Sabido
O
O
Beauchesne
P
P
Dubois
F
F
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D001932:Brain Neoplasms / Q000378:metabolism*; D005434:Flow Cytometry; D019450:Genes, MDR / Q000235:genetics*; D005910:Glioma / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009943:Organophosphorus Compounds / Q000378:metabolism*; D015609:Organotechnetium Compounds / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000378:metabolism*; D017256:Technetium Tc 99m Sestamibi / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005978:Glutathione; D007074:Immunoglobulin G; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D017256:Technetium Tc 99m Sestamibi; C078700:technetium tc-99m tetrofosmin