DNA interaction with rabbit sperm cells and its transfer into ova in vitro and in vivo.
Exogenous DNA interaction with rabbit sperm cells and its transfer into ova has been studied. It was shown that rabbit sperm cells are able to capture, hold, and transfer exogenous DNA to ova during fertilization. Sperm/DNA transfer leads to genetically transformed offspring. Transfection efficiency varied within a large range. Low expression and rearrangements of the transgene were observed.
Kuznetsov
A V
AV
State Research Center for Applied Microbiology, Obolensk, Russia.
Kuznetsova
I V
IV
Schit
I Y
IY
D000818:Animals; D030801:Animals, Genetically Modified; D002467:Cell Nucleus; D006224:Cricetinae; D004274:DNA, Recombinant / Q000235:genetics* / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007315:Insemination, Artificial; D008297:Male; D008647:Mesocricetus; D008845:Microinjections; D010063:Ovum / Q000166:cytology*; D016133:Polymerase Chain Reaction; D011817:Rabbits; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000166:cytology*; D014162:Transfection
D004274:DNA, Recombinant