Application of the intracellular gamma interferon assay to recalculate the potency of CD8(+) T-cell responses to herpes simplex virus.
Enumeration and characterization of herpes simplex virus (HSV)-specific CD8(+) T lymphocytes are tedious and indirect. We quantitated antigen-specific CD8(+) T cells during acute and secondary stages of HSV infection using intracellular gamma interferon production upon stimulation with virus or immunodominant peptide. Results show a substantial increase in the number of CD8(+) T cells which was otherwise underestimated with the conventional limiting dilution analysis.
Kumaraguru
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Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996-0845, USA.
Rouse
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BT
D000208:Acute Disease; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D002478:Cells, Cultured; D006561:Herpes Simplex / Q000276:immunology* / Q000821:virology; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology; D007371:Interferon-gamma / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000276:immunology* / Q000502:physiology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000821:virology
D015415:Biomarkers; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D007371:Interferon-gamma