title: 10q23.3 loss of heterozygosity is higher in lymph node-positive (pT2-3,N+) versus lymph node-negative (pT2-3,N0) prostate cancer.
abstract: Loss of heterozygosity (LOH) in the region of 10q23.3 has been associated with multiple tumors, including glioblastoma multiforme, melanoma, endometrial carcinoma, and prostate carcinoma. The tumor suppressor gene, PTEN/MMAC1, is also located in this region, and, in addition to other tumor types (eg, glioblastoma multiforme, endometrial, and melanoma), PTEN/MMAC1 mutations have been found in prostate cancer cell lines, xenografts, and hormone refractory prostate cancer tissue specimens. The aim of this study was to evaluate LOH at 10q23.3 as a marker of cancer progression in node-positive prostate cancer. Genetic alterations in the region of 10q23.3 were assessed in 23 node-positive (pT2-3, N+) and 44 node-negative prostate (pT2-3, N0) cancers with D10S532, D10S1687, D10S541, and D10S583 flanking polymorphic genetic markers; PTENCA, a genetic marker within PTEN/MMAC1, was also tested. Using DNA from paired normal and microdissected tumor samples, LOH at microsatellite loci was determined after polymerase chain reaction amplification. LOH in at least 1 marker was identified in 14% (6 of 44) of lymph node-negative and 43% (10 of 23) of lymph node-positive prostate cancers (chi-square test, P = .007). This increase in genetic alterations in node-positive prostate cancer suggests that 10q23.3 is a marker for metastatic progression.
authors.affiliation: Department of Pathology and Medicine, College of Physicians and Surgeons of Columbia University, New York, NY, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Rubin
authors.forename: A
authors.initials: A
authors.lastname: Gerstein
authors.forename: K
authors.initials: K
authors.lastname: Reid
authors.forename: D G
authors.initials: DG
authors.lastname: Bostwick
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: R
authors.initials: R
authors.lastname: Parsons
authors.forename: N
authors.initials: N
authors.lastname: Papadopoulos
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000556:secondary; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics*; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology