Large unilamellar vesicles as trehalose-stabilised vehicles for vaccines: storage time and in vivo studies.
Liposomes, as a pharmaceutical formulation must display a long shelf life. The recombinant heat-shock protein from Mycobacterium leprae (18-kDa hsp) or its N-acylated derivative, when entrapped within or externally associated with large unilamellar vesicles, acts as a T-epitope source. Freeze-fracture electron microscopy shows unequivocally that trehalose avoids aggregation and fusion of these vesicles. Formulations containing trehalose retained up to 98% of the entrapped protein. The highest antibody level is obtained with formulations containing trehalose. The adjuvant effect depends on the liposomal membrane integrity.
Quintilio
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W
Lab. de Microesferas e Lipossomos, C. de Biotecnologia, I. Butantan, Av. Vital Brasil 1500, 05503-900, Butantan, Brazil.
Sato
R A
RA
Sant'Anna
O A
OA
Esteves
M I
MI
Sesso
A
A
de Araujo
P S
PS
Bueno da Costa
M H
MH
D000215:Acylation; D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay; D005614:Freeze Fracturing; D006360:Heat-Shock Proteins / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis; D008081:Liposomes; D008567:Membranes, Artificial; D051379:Mice; D009166:Mycobacterium leprae / Q000276:immunology*; D014677:Pharmaceutical Vehicles; D014199:Trehalose / Q000737:chemistry*
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008081:Liposomes; D008567:Membranes, Artificial; D014677:Pharmaceutical Vehicles; C081475:hsp18 protein, bacteria; D014199:Trehalose