title: The Candida albicans antiporter gene CNH1 has a role in Na+ and H+ transport, salt tolerance, and morphogenesis.
abstract: The isolation and functional characterization of a Candida albicans Na+/H+ antiporter gene, CNH1, is reported here. The gene encodes a protein of 840 amino acids that exhibits high levels of similarity in sequence, size, and structural and functional domains to a group of known Na+/H+ antiporters of fungi. The CNH1 gene is able to functionally complement the salt-sensitivity of a Saccharomyces cerevisiae ena1 nha1 mutant, and mutations of two conserved aspartate residues to asparagines in the putative Na+-binding site abolish this activity. Deletion of CNH1 results in retardation of growth and a highly elongated morphology in a significant fraction of cells under conditions that normally support yeast growth. These results indicate that CNH1 has a role in Na+ and H+ transport, salt-tolerance, and morphogenesis.
authors.affiliation: Microbial Collection and Screening Laboratory, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 1176091.
authors.forename: Tuck-Wah
authors.initials: TW
authors.lastname: Soong
authors.affiliation: Microbial Collection and Screening Laboratory, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 1176091.
authors.forename: Tan-Fong
authors.initials: TF
authors.lastname: Yong
authors.affiliation: Microbial Collection and Screening Laboratory, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 1176091.
authors.forename: Narendrakumar
authors.initials: N
authors.lastname: Ramanan
authors.affiliation: Microbial Collection and Screening Laboratory, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 1176091.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D027682:Cation Transport Proteins; D003001:Cloning, Molecular; D005690:Galactose; D017353:Gene Deletion; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase; D014170:Transformation, Genetic
chemical_list: D027682:Cation Transport Proteins; C086375:ENA1 protein, S cerevisiae; D008565:Membrane Proteins; C000631624:NHA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C490997:Slc9a1 protein, mouse; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D005690:Galactose