title: D(Va) category phenotype and genotype in Japanese families.
abstract: OBJECTIVE
  The genetic background of the D(Va) category phenotype has been described in two Caucasian individuals. We were interested in the RHD sequence of 7 Japanese D(Va) individuals and their families.
  
  
  METHODS
  With SSP-PCR we tested exons 4, 5 and 7 of the RHD gene and used restriction enzymes for testing nucleotide associated with the D(Va) phenotype.
  
  
  RESULTS
  A single RHD G667 C697 allele was present in 5 individuals with D(Va) category phenotype, and in 2 individuals we found a D-CE-D hybrid gene (exon 5 had been replaced). The D(Va)Ce gene complex was found in all families.
  
  
  CONCLUSIONS
  The changes of the RHD gene described in European D(Va) individuals were also observed in Japanese families.
authors.affiliation: Department of Transfusion Medicine, University of Göttingen, Germany. tlegler@med.uni-goettingen.de
authors.forename: T J
authors.initials: TJ
authors.lastname: Legler
authors.forename: V
authors.initials: V
authors.lastname: Wiemann
authors.forename: H
authors.initials: H
authors.lastname: Ohto
authors.forename: I
authors.initials: I
authors.lastname: Matuda
authors.forename: T
authors.initials: T
authors.lastname: Obara
authors.forename: M
authors.initials: M
authors.lastname: Uchikawa
authors.forename: M
authors.initials: M
authors.lastname: Köhler
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D005091:Exons; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D010375:Pedigree; D010641:Phenotype; D018807:Polymorphism, Single-Stranded Conformational; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D012698:Serologic Tests
chemical_list: D012204:Rh-Hr Blood-Group System