Increased uptake of 99mTc-HL91 in tumor cells exposed to X-ray radiation.
99mTc-HL91, a hypoxic marker, may be a predictor of tumor response to radiotherapy and an indicator of tumor oxygenation in the course of treatment. In this study, serial changes in 99mTc-HL91 uptake were observed in the normoxic condition in a human bladder cancer cell line exposed to a single dose or a fractionated dose of 10 Gy with an x-ray beam. The uptake per cell increased during cell growth retardation induced by the irradiation. This finding indicates that 99mTc-HL91 uptake is affected by injury to cells due to radiation; it may therefore be difficult to correctly assess the tissue oxygenation status during radiotherapy with 99mTc-HL91.
Kinuya
S
S
Department of Nuclear Medicine, Kanazawa University School of Medicine, Ishikawa, Japan. kinuya@med.kanazawa-u.ac.jp
Yokoyama
K
K
Konishi
S
S
Li
X F
XF
Watanabe
N
N
Shuke
N
N
Aburano
T
T
Takayama
T
T
Michigishi
T
T
Tonami
N
N
D002455:Cell Division / Q000528:radiation effects; D015687:Cell Hypoxia; D006801:Humans; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D010091:Oximes / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000532:radiotherapy*
D015609:Organotechnetium Compounds; D010091:Oximes; D019275:Radiopharmaceuticals; C105527:technetium Tc 99m 4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime