Heparin- and Zn2+-binding proteins from boar seminal plasma.
Low molecular mass, heparin-binding proteins from seminal plasma play an important role in gametes interaction whereas plasmatic Zn2+-binding proteins stabilize chromatin and plasmalemma structures and protect spermatozoa in the female reproductive tract. By means of affinity chromatography the heparin- and Zn2+-binding proteins were isolated from boar seminal plasma and both preparations were analyzed by reverse HPLC. Most of the proteins bound to heparine and Zn2+-ions were classified as spermadhesins. Three fractions binding exclusively Zn2+ were isolated. They differ in amino-acid composition, content of glucosamine and content of protein components revealed by SDS/PAGE.
Hołody
D
D
Department of Animal Biochemistry, Olsztyn University of Agriculture and Technology, Olsztyn-Kortowo, Poland. dholody@art.olsztyn.pl
Strzezek
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D026563:LDL-Receptor Related Protein-Associated Protein; D008297:Male; D012661:Semen / Q000378:metabolism*; D013552:Swine; D015032:Zinc / Q000378:metabolism*
D000596:Amino Acids; D002352:Carrier Proteins; D006023:Glycoproteins; D026563:LDL-Receptor Related Protein-Associated Protein; C017800:zinc-binding protein; D006493:Heparin; D015032:Zinc