Composition of N-linked carbohydrates from ovalbumin and co-purified glycoproteins.
Analysis of commercial samples of chicken ovalbumin by reversed-phase high performance liquid chromatography and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) showed the presence of several other co-purifying glycoproteins. These were isolated, subjected to tryptic digestion, and two of them were identified as ovomucoid and chicken riboflavin binding-protein following database matching of the peptide masses obtained by MALDI. The N-linked glycans were released from the glycoproteins and their structures were examined by MALDI-MS in combination with exoglycosidase digestion. Ovalbumin was found to be glycosylated mainly with high-mannose and hybrid structures, consistent with profiles obtained on the intact glycoprotein by electrospray. The other glycoproteins contained mainly larger, complex glycans with up to five antennae, many of which had earlier been associated with ovalbumin.
Harvey
D J
DJ
Department of Biochemistry, Oxford Glycobiology Institute, United Kingdom. dh@glycob.ox.ac.uk
Wing
D R
DR
Küster
B
B
Wilson
I B
IB
D002241:Carbohydrates / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D006868:Hydrolysis; D013058:Mass Spectrometry; D010047:Ovalbumin / Q000737:chemistry* / Q000302:isolation & purification; D010455:Peptides / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D014357:Trypsin
D002241:Carbohydrates; D006023:Glycoproteins; D006834:Hydrazines; D010455:Peptides; D011134:Polysaccharides; D010047:Ovalbumin; D006026:Glycoside Hydrolases; D014357:Trypsin