Synthesis of oligodeoxyribonucleotide bearing 2'-S-alkyl residue and its effect on the duplex stability.
2'-Deoxy-2'-S-hexyluridine derivative was synthesized from 2,2'-anhydrouridine and 1-hexanethiol and incorporated into an oligodeoxyribonucleotide. The thermal stability of the duplexes formed by the 2'-S-hexyl modified ODN with either the complementary DNA or RNA strand was decreased compared to the unmodified counterparts.
Ozaki
H
H
Department of Chemistry, Faculty of Engineering, Gunma University, Japan.
Sato
Y
Y
Azuma
S
S
Sawai
H
H
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002942:Circular Dichroism; D018076:DNA, Complementary / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry*; D018075:RNA, Complementary / Q000737:chemistry; D013816:Thermodynamics; D013872:Thionucleosides / Q000138:chemical synthesis* / Q000737:chemistry
C412235:3'-O-((2-cyanoethyl)(diisopropylamino))phosphino-5'-O-(4,4'-dimethoxytrityl)-2'-deoxy-2'-S-hexyluridine; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D018075:RNA, Complementary; D013872:Thionucleosides