A preliminary study of single nucleotide polymorphisms of lipoprotein lipase gene in coronary atherosclerotic heart disease.
OBJECTIVE
This study inquired into the relationship between single nucleotide polymorphisms(SNPs) of lipoprotein lipase gene (LPL) and coronary atherosclerotic heart disease(CHD).


METHODS
Exon 6 and fragment of intron 6 in LPL gene from 102 cases of CHD and 110 normal subjects were analysed by polymerase chain reaction amplification, temperature-modulated high-performance liquid chromatography (TmHPLC) and DNA sequencing.


RESULTS
Three new SNPs (4212t/c, 4509t/c, 4576a/c) were detected in the intron 6 of LPL gene. There was significant difference in frequencies of 4212t/c, and 4576a/c between health group and CHD group(P<0.05).


CONCLUSIONS
This study has accumulated additional data on SNPs in LPL gene and provided new data for exploring the mechanism of CHD.
Su
Z
Z
Department of Medical Genetics, The First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan, 610041 P. R. China. zszg@wcums.edu.cn
Zhang
S
S
Hou
Y
Y
Zhang
L
L
Liao
L
L
Xia
Q
Q
Xiao
C
C
Meng
H
H
Yan
Y
Y
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D006801:Humans; D008071:Lipoprotein Lipase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013696:Temperature
D008071:Lipoprotein Lipase