Acquisition of intact allogeneic human leukocyte antigen molecules by human dendritic cells.
In an attempt to transduce monocyte-derived dendritic cells (DCs) by a retroviral vector coding for a cell surface marker, we were confronted by the observation of high transfer of the surface molecule in the absence of vector proviral DNA in the treated cells. Indeed, DCs acquired the surface marker by a mechanism independent of the vector machinery, requiring cell-to-cell contact and involving transfer of lipids and a variety of intact membrane proteins. Most important, this property of DCs also includes acquisition of foreign human leukocyte antigen (HLA) molecules. Consequently, DCs become immunological hybrids as they display their own and foreign HLA molecules. The newly acquired HLA is fully functional because it allows recognition by allo-specific T lymphocytes and the binding and presentation of antigen peptides.
Russo
V
V
HSR-Telethon Institute of Gene Therapy (TIGET) and the Cancer Immunotherapy and Gene Therapy Program, H.S. Raffaele Scientific Institute, Milan, Italy.
Zhou
D
D
Sartirana
C
C
Rovere
P
P
Villa
A
A
Rossini
S
S
Traversari
C
C
Bordignon
C
C
D002450:Cell Communication / Q000502:physiology; D002462:Cell Membrane / Q000276:immunology / Q000502:physiology; D003713:Dendritic Cells / Q000276:immunology*; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D007519:Isoantigens / Q000378:metabolism*; D008545:Melanoma; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D006684:HLA-DR Antigens; D007519:Isoantigens; D008563:Membrane Lipids; D008565:Membrane Proteins