Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. Strain NG-27.
Alkalophilic Bacillus sp. strain NG-27 produces a 42-kDa endoxylanase active at 70 degrees C and at a pH of 8.4. The gene for this endoxylanase was cloned and sequenced. The gene contained one open reading frame of 1,215 bases. An active site characteristic of the family 10 beta-glycanases was recognized between amino acids 303 and 313, with the active glutamate at position 310. Though highly thermostable, the enzyme contains no cysteine residue.
Gupta
N
N
Institute of Microbial Technology, Sector 39-A, Chandigarh-160036, India.
Reddy
V S
VS
Maiti
S
S
Ghosh
A
A
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D043364:Endo-1,4-beta Xylanases; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013696:Temperature; D014995:Xylosidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases