Measurement of urinary retinol binding protein by immunonephelometry.
A method is described for the determination of retinol binding protein (RBP) by immunonephelometry. The assay is sensitive to 5 microg/l and has acceptable imprecision. The method correlates with an established ELISA assay. A provisional normal range is proposed for daytime random urine samples. The increased excretion of RBP in adult subject with type 1 diabetes mellitus is demonstrated.
Twyman
S J
SJ
Department of Chemical Pathology, Southampton General Hospital, SO16 6YD, Southampton, UK.
Overton
J
J
Rowe
D J
DJ
D000293:Adolescent; D000328:Adult; D003922:Diabetes Mellitus, Type 1 / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D012177:Retinol-Binding Proteins / Q000592:standards / Q000652:urine*
D012177:Retinol-Binding Proteins