Rapid determination of membrane transport parameters in adherent cells.
Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials. The method is convenient, cost effective and requires no special apparatus. Initial uptake rates, steady-state and free substrate levels are demonstrated in ZR-75-1 breast cancer and Chinese hamster ovary cell lines using methotrexate, a model agent transported by the reduced folate carrier. The technique should be applicable to the study of the transport properties in a broad range of substrates and cells in monolayer culture.
Sharif
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KA
Comprehensive Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Goldman
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ID
D000818:Animals; D001943:Breast Neoplasms; D016466:CHO Cells; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000502:physiology; D006224:Cricetinae; D015195:Drug Design; D004345:Drug Industry / Q000379:methods*; D005493:Folic Acid Antagonists / Q000493:pharmacokinetics*; D006801:Humans; D008727:Methotrexate / Q000493:pharmacokinetics*; D012588:Scintillation Counting; D014162:Transfection; D014316:Tritium; D014407:Tumor Cells, Cultured
D005493:Folic Acid Antagonists; D014316:Tritium; D008727:Methotrexate