14-3-3 proteins interact with a 13-lipoxygenase, but not with a 9-lipoxygenase.
Associations between lipoxygenases (Lox) and 14-3-3 proteins were demonstrated by two different methods. First, immunoprecipitation experiments, using isoenzyme-specific monoclonal Lox antibodies, showed that 14-3-3 proteins co-precipitate with 13-Lox, but not with the 9-Lox from barley. Second, interactions between 13-Lox and 14-3-3 were established by surface plasmon resonance studies, showing that 13-Lox binds with 14-3-3 proteins in a concentration-dependent manner. The interactions between 14-3-3 proteins and 13-Lox may reveal their role during plant development.
Holtman
W L
WL
Center for Phytotechnology UL-TNOO, Department of Applied Plant Sciences, Wassenaarseweg 64, 2333 Al Leiden, The Netherlands. holtman@voeding.tno.nl
Roberts
M R
MR
Oppedijk
B J
BJ
Testerink
C
C
van Zeijl
M J
MJ
Wang
M
M
D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004926:Escherichia coli; D001467:Hordeum / Q000201:enzymology; D007163:Immunosorbent Techniques; D007527:Isoenzymes / Q000378:metabolism*; D008084:Lipoxygenase / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011506:Proteins; D011994:Recombinant Proteins; C117433:13-lipoxygenase; D008084:Lipoxygenase; D014446:Tyrosine 3-Monooxygenase