Preparation of completely 6-O-desulfated heparin and its ability to enhance activity of basic fibroblast growth factor.
Although regioselective removal of 6-O-sulfate groups of heparin has been undertaken by several researchers, complete 6-O-desulfation with little side reaction has not been attained successfully. In this work, a modified method with a certain silylating reagent, N-methyl-N-(trimethylsilyl)trifluoroacetamide, has been established to produce completely 6-O-desulfated heparin with few other chemical changes. The degrees of 6-O-desulfation were estimated by means of chemical disaccharide analyses and/or (13)C NMR spectra. Although the completely 6-O-desulfated heparin lost about 20% of 2-O-sulfate groups, any other chemical changes and depolymerization were not detected. The completely 6-O-desulfated heparin displayed strong inhibition of COS-1 cell adhesion to basic fibroblast growth factor (bFGF)-coated well in a dose-dependent manner, as was clarified by the competitive cell-adhesion assay. Furthermore, the completely 6-O-desulfated heparin was shown to promote in vitro A31 fibroblast proliferation in a dose-dependent manner in the presence of bFGF. These results suggest that signal transduction through bFGF/bFGF receptor in A31 cells occurs in the absence of 6-O-sulfate groups in heparin. The involvement of 6-O-sulfate group(s) of heparin/heparan sulfate in the promotion of bFGF mitogenic activity was reported by several groups. This discrepancy between our results and those of other groups would be due to the differences in molecular size of heparin/heparan sulfate derivatives and/or cell species used for the assay.
Kariya
Y
Y
Tokyo Research Institute, Seikagaku Corporation, 3-1253 Tateno, Higashiyamato, Tokyo 207-0021, Japan. kariya@to.sekagaku.co.jp
Kyogashima
M
M
Suzuki
K
K
Isomura
T
T
Sakamoto
T
T
Horie
K
K
Ishihara
M
M
Takano
R
R
Kamei
K
K
Hara
S
S
D000818:Animals; D019556:COS Cells; D002455:Cell Division; D002851:Chromatography, High Pressure Liquid; D019281:Dimerization; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006493:Heparin / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008938:Mitosis; D013431:Sulfates
D013431:Sulfates; D016222:Fibroblast Growth Factor 2; D006493:Heparin