Influence of lipid peroxidation on the nicotinic acetylcholine receptors in PC12 cells.
The expression of neuronal nicotinic acetylcholine receptors (nAChRs) influenced by lipid peroxidation has been investigated. The results showed that exposure of PC12 cells to a free radical insult (FeSO(4)) induced concentration-dependent increase in level of MDA (malondialdehyde), and a significant decline in MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] reduction. In cultured cells exposed to FeSO(4), a significant dose-dependent decrease in [(3)H] epibatidine binding sites was detected and a significant reduction in B(max) value was observed in the high affinity epibatidine binding site. These results demonstrate that lipid peroxidation in cellular membranes can induce a reduction in numbers of neuronal nAChRs.
Guan
Z
Z
Department of Clinical Neuroscience, Occupational Therapy and Elderly Care Research, Division of Molecular Neuropharmacology, Huddinge University Hospital B84, Karolinska Institutet, S-141 86, Huddinge, Sweden. zhizhong@kfcmail.hs.sll.se
Zhang
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Nordberg
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D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005296:Ferrous Compounds / Q000494:pharmacology; D008054:Lipid Peroxides / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D018722:Nicotinic Agonists / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D016716:PC12 Cells / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism; D051381:Rats; D011978:Receptors, Nicotinic / Q000187:drug effects*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D019086:Bridged Bicyclo Compounds, Heterocyclic; D005296:Ferrous Compounds; D008054:Lipid Peroxides; D018722:Nicotinic Agonists; D011725:Pyridines; D011978:Receptors, Nicotinic; D013778:Tetrazolium Salts; D013844:Thiazoles; C020748:ferrous sulfate; D008315:Malondialdehyde; C022616:thiazolyl blue; C082748:epibatidine