title: GAP-43 N-terminal translocation signal targets beta-galactosidase to developing axons in a pan-neuronal transgenic mouse line.
abstract: Tracing neural connectivity is important in understanding the intricacy of the nervous system as this represents the functional unit throughout the system. Here, we provide evidence that beta-galactosidase (beta-gal) linked to the N-terminal axonal translocation signal of GAP-43 provides a reproducible and versatile reporter system for analyzing the developing nervous system in vivo. When expressed by the GAP-43 promoter in transgenic mice, the fusion protein is detected equally within the developing axons of the peripheral and the central nervous systems, directly reflecting the promoter activity.
authors.affiliation: Department of Anatomy, Asahikawa Medical College, 4-5-3-11, Nishikagura, Asahikawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: N D
authors.initials: ND
authors.lastname: Allen
authors.forename: P C
authors.initials: PC
authors.lastname: Emson
authors.forename: H
authors.initials: H
authors.lastname: Kiyama
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D019922:GAP-43 Protein / Q000235:genetics*; D005697:Galactosides / Q000235:genetics / Q000378:metabolism*; D017950:Ganglia, Sensory / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D020439:Growth Cones / Q000378:metabolism; D007763:Lac Operon; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D021382:Protein Sorting Signals / Q000235:genetics*
chemical_list: D019922:GAP-43 Protein; D005697:Galactosides; D021382:Protein Sorting Signals; C007816:beta-galactoside