Anti-rat CD45RA monoclonal antibodies cross-react with glucagon.
Anti-rat CD45RA monoclonal antibody (MAb) has been used as a marker for rat B-lymphocytes (B-cells). When we applied the MAb to identify B-lymphocytes among immune cells infiltrating pancreatic islets of the BB rat, which is an animal model for human type 1 diabetes mellitus, a subpopulation of islet cells was immunostained as well. Our immunohistochemical investigations demonstrated that the stained islet cells are pancreatic alpha-cells and the MAb cross-reacts with glucagon.
Kaino
Y
Y
Department of Pediatrics, Ehime University School of Medicine, Shigenobu, Japan. kaino@m.ehime-u.ac.jp
Ito
T
T
Hirai
H
H
Kida
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D003429:Cross Reactions; D005934:Glucagon / Q000276:immunology*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD; D010179:Pancreas / Q000473:pathology; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D051381:Rats; D011913:Rats, Inbred BB; D011917:Rats, Inbred Lew
D000911:Antibodies, Monoclonal; D005934:Glucagon; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1