Factors influencing the expression of interleukin-1 beta in cultured rainbow trout (Oncorhynchus mykiss) leucocytes.
The dose dependency and kinetics of lipopolysaccharide (LPS) induced interleukin-1beta (IL-1beta) mRNA expression in rainbow trout leucocytes has been studied. Northern blot analysis revealed weak hybridisation of a trout IL-1beta probe to RNA from head kidney leucocytes stimulated with 0.1 microg LPS/ml, with a large increase in IL-1beta transcript level seen between 0.1 and 1.5 microg LPS/ml. Using 5 microg LPS/ml expression was first detectable 1-2 h post-stimulation. By 4 h post-stimulation maximal induction was seen but by 24-48 h the level had fallen and by 72 h no transcript was detectable. Culture temperature had a marked effect on IL-1beta expression, with low temperatures inhibiting transcription. Indeed, an eight-fold increase in IL-1beta transcript level was seen between cells cultured at 4 degrees C and 22 degrees C. Preincubation with cortisol was also shown to inhibit LPS-induced IL-1beta expression.
Zou
J
J
Department of Zoology, University of Aberdeen, UK.
Holland
J
J
Pleguezuelos
O
O
Cunningham
C
C
Secombes
C J
CJ
D000818:Animals; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D006854:Hydrocortisone / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007668:Kidney / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017686:Oncorhynchus mykiss / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013696:Temperature
D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D006854:Hydrocortisone