Application of a luminescence-based pH optrode to monitoring of fermentation by Klebsiella pneumoniae.
The application of an optical sensor based on immobilization of the ruthenium complex [Ru(bpy)2(dhphen)]2+ (bpy = 2,2'-bipyridine, dhphen = 4,7-dihydroxy-1,10-phenanthroline) in Nafion to pH monitoring of fermentation by Klebsiella pneumoniae is described. Interference from the culture medium can be eliminated by addition of a black microporous filter membrane (pore size: 0.45 microm) on top of the sensing film. The response of this pH optrode was found to show good correlation with the conventional pH electrode.
Chan
C M
CM
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hunghom, Kowloon.
Lo
W
W
Wong
K Y
KY
D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D008163:Luminescent Measurements