title: Construction and evaluation of a capillary array DNA sequencer based on a micromachined sheath-flow cuvette.
abstract: A capillary array electrophoresis DNA sequencer is reported based on a micromachined sheath-flow cuvette as the detection chamber. This cuvette is equipped with a set of micromachined features that hold the capillaries in precise registration to ensure uniform spacing between the capillaries, in order to generate uniform hydrodynamic flow in the cuvette. A laser beam excites all of the samples simultaneously, and a microscope objective images fluorescence onto a set of avalanche photodiodes, which operate in the analog mode. A high-gain transimpedance amplifier is used for each photodiode, providing high duty-cycle detection of fluorescence.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada.
authors.forename: H J
authors.initials: HJ
authors.lastname: Crabtree
authors.forename: S J
authors.initials: SJ
authors.lastname: Bay
authors.forename: D F
authors.initials: DF
authors.lastname: Lewis
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: L D
authors.initials: LD
authors.lastname: Coulson
authors.forename: G A
authors.initials: GA
authors.lastname: Fitzpatrick
authors.forename: S L
authors.initials: SL
authors.lastname: Delinger
authors.forename: D J
authors.initials: DJ
authors.lastname: Harrison
authors.forename: N J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005069:Evaluation Studies as Topic; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods
chemical_list: D004279:DNA, Viral; D004247:DNA