Comedogenicity of squalene monohydroperoxide in the skin after topical application.
The comedogenicity of squalene peroxides was examined on the rabbit ear skin after topical application of squalene-monohydroperoxide (Sq-OOH), the initial product when squalene was irradiated with UV-A. Since comedogenic products from UV-irradiated squalene were extracted with methanol solution, we isolated Sq-OOH by reverse-phased HPLC with a methanol mobile phase solvent. The degree of comedogenic reaction induced by Sq-OOH was higher than that of well-known comedogenic cosmetic ingredients. Unlike two other mono-peroxides, tert-butyl hydroperoxide and cumene-mono-hydroperoxide, Sq-OOH induced comedo-formation in the rabbit ear skin. However, the comedogenicity of reduced Sq-OOH, squalene-hydroxide (Sq-OH) and squalene itself was lower than that of Sq-OOH. These results indicate that Sq-OOH is a potent comedogenic mono-hydroperoxide chemical to rabbit skin.
Chiba
K
K
Department of Toxicology, Safety Research Center, Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Yoshizawa
K
K
Makino
I
I
Kawakami
K
K
Onoue
M
M
D000152:Acne Vulgaris / Q000139:chemically induced* / Q000473:pathology; D000287:Administration, Topical; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004431:Ear, External / Q000187:drug effects / Q000473:pathology; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000138:chemical synthesis / Q000633:toxicity*; D008054:Lipid Peroxides / Q000633:toxicity; D008297:Male; D010782:Photolysis; D011817:Rabbits; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D013185:Squalene / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000528:radiation effects / Q000633:toxicity*; D018675:Toxicity Tests; D014466:Ultraviolet Rays
D008054:Lipid Peroxides; D013185:Squalene; D006861:Hydrogen Peroxide