Diversification and selection mechanisms for the production of protein repertoires: lessons from the immune system.
The physiological mechanism for producing antigen-specific antibodies is based on a two-phase neo-Darwinian process: the first phase consists of diversity generation (formation of the repertoire), and the second phase is antigen-mediated selection. In this article, we consider how the natural immunoglobulin gene-diversification processes can be exploited both in vivo and in vitro in order to allow the generation of novel antibody (and heterologous protein) repertoires.
Neuberger
M S
MS
Medical Research Council Laboratory of Molecular Biology, Cambridge, NSW, Australia. msn@mrc-lmb.cam.ac.uk
Sale
J E
JE
Cumbers
S J
SJ
Jolly
C J
CJ
Bemark
M P
MP
Ehrenstein
M R
MR
Lanoue
A
A
Brüggemann
M
M
Batista
F D
FD
Davies
S L
SL
Williams
G T
GT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics; D000915:Antibody Affinity / Q000235:genetics; D000916:Antibody Diversity / Q000235:genetics*; D000918:Antibody Specificity / Q000235:genetics; D002460:Cell Line; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D012641:Selection, Genetic
D000911:Antibodies, Monoclonal