Studies on hepatic gene expression in different liver regenerative models.
We have investigated the expression of several growth-related genes in the liver after partial hepatectomy in three experimental models: normal, Dexamethasone-pretreated, and hypophysectomized rats. Dexamethasone and hypophysectomy resulted in a delay in the peak of cell replication in 6 and 18 h, respectively, when compared to the normal animals. TGFalpha mRNA expression was shifted together with the DNA synthesis, but the expression of c-myc, c-fos, c-jun, HGF, TGFbeta1, IL1beta did not delay. This result suggests that liver-derived TGFalpha but not the other factors are important in the timing of the proliferative response after partial hepatectomy.
Nagy
P
P
First Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest, Hungary. peter_nagy@nih.gov
Bisgaard
H C
HC
Schnur
J
J
Thorgeirsson
S S
SS
D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D019925:Cyclin A / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D006498:Hepatectomy; D017228:Hepatocyte Growth Factor / Q000235:genetics; D007016:Hypophysectomy; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology*; D008115:Liver Regeneration / Q000187:drug effects; D008297:Male; D008954:Models, Biological; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors; D015291:Transforming Growth Factors / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D019925:Cyclin A; D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D017228:Hepatocyte Growth Factor; D015291:Transforming Growth Factors; D003907:Dexamethasone; D016203:CDC2 Protein Kinase