Co-localization of TFF3 peptide and oxytocin in the human hypothalamus.
TFF-peptides (formerly P domain peptides, trefoil factors) are typical secretory products of many mucous epithelial cells. TFF3 is also synthesized in the hypothalamus and has anxiolytic or anxiogenic activities when injected into the rat amygdala. Here we show by immunohistochemistry that TFF3 is localized to a distinct population of neurons of the human hypothalamic paraventricular and supraoptic nuclei. Generally, TFF3-positive cells are co-localized in oxytocin-producing cells and not in vasopressin-producing cells. Relatively large amounts of TFF3-but not TFF1 and TFF2-are present in the posterior lobe of the human pituitary, where it is probably released into the bloodstream. Furthermore, TFF3 was also detectable in human postmortem cerebrospinal fluid.
Jagla
W
W
Institut für Molekularbiologie und Medizinische Chemie, Otto-von-Guericke-Universität, D-39120 Magdeburg, Germany.
Wiede
A
A
Dietzmann
K
K
Rutkowski
K
K
Hoffmann
W
W
D015153:Blotting, Western; D002555:Cerebrospinal Fluid / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D009077:Mucins; D009124:Muscle Proteins; D009474:Neurons / Q000737:chemistry*; D010121:Oxytocin / Q000032:analysis*; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry* / Q000166:cytology; D010455:Peptides; D010904:Pituitary Gland, Posterior / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D013495:Supraoptic Nucleus / Q000737:chemistry* / Q000166:cytology; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014667:Vasopressins / Q000032:analysis
D009077:Mucins; D009124:Muscle Proteins; D010455:Peptides; D011506:Proteins; C000604007:TFF2 protein, human; C082495:TFF3 protein, human; D000071164:Trefoil Factor-2; D000071165:Trefoil Factor-3; D014667:Vasopressins; D010121:Oxytocin