Exposure of plasma proteins on Dacron and ePTFE vascular graft material in a perfusion model.
OBJECTIVE
to compare the exposure of plasma proteins adsorbed onto three vascular graft materials (polytetrafluoroethylene ePTFE and two modifications of polyethyleneterephthalate Dacron).


METHODS
surface exposure of fibronectin, vitronectin, thrombospondin, antithrombin III, IgG, high molecular-weight kininogen, fibrinogen, albumin and plasminogen was studied by incubation with radiolabelled antibodies in a perfusion model. Perfusion times with human plasma were 1, 4, 24 and 48 hours.


RESULTS
all proteins could be detected at 1, 4, 24 and 48 hours after the start of perfusion. Overall, the least amount of proteins adsorbed onto ePTFE.


CONCLUSIONS
the low adsorption of proteins onto ePTFE may be one of the reasons for the lower incidence of infections reported with this material.
Falkenback
D
D
Department of Infectious Diseases and Medical Microbiology, Lund University, Lund, Sweden.
Lundberg
F
F
Ribbe
E
E
Ljungh
A
A
D000327:Adsorption; D001798:Blood Proteins / Q000378:metabolism*; D001807:Blood Vessel Prosthesis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D066298:In Vitro Techniques; D008954:Models, Biological; D010477:Perfusion / Q000379:methods; D011093:Polyethylene Terephthalates / Q000378:metabolism*; D011138:Polytetrafluoroethylene / Q000378:metabolism*; D011485:Protein Binding
D001798:Blood Proteins; D007074:Immunoglobulin G; D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene