title: Functional analysis of the genomic and antigenomic promoters of human respiratory syncytial virus.
abstract: The promoters involved in transcription and RNA replication by respiratory syncytial virus (RSV) were examined by using a plasmid-based minireplicon system. The 3' ends of the genome and antigenome, which, respectively, contain the 44-nucleotide (nt) leader (Le) and 155-nt trailer-complement (TrC) regions, should each contain a promoter for RNA replication. The 3' genome end also should have the promoter for transcription. Substitution for the Le with various lengths of TrC demonstrated that the 3'-terminal 36 nt of TrC are sufficient for extensive (but not maximal) replication and that when juxtaposed with a transcription gene-start (GS) signal, this sequence was also able to direct transcription. It was also shown that the region of Le immediately preceding the GS signal of the first gene could be deleted with either no effect or with a slight decrease in transcription initiation. Thus, the TrC is competent to direct transcription even though it does not do so in nature, and the partial sequence identity it shares with the 3' end of the genome likely represents the important elements of a conserved promoter active in both replication and transcription. Increasing the length of the introduced TrC sequence incrementally to 147 nt resulted in a fourfold increase in replication and a nearly complete inhibition of transcription. These two effects were unrelated, implying that transcription and replication are not interconvertible processes mediated by a common polymerase, but rather are independent processes. The increase in replication was specific to the TrC sequence, implying the presence of a nonessential, replication-enhancing cis-acting element. In contrast, the inhibitory effect on transcription was due solely to the altered spacing between the 3' end of the genome and GS signal, which implies that the transcriptase recognizes the first GS signal as a promoter element. Neither the enhancement of replication nor the inhibition of transcription was due to increased base-pairing potential between the 3' and 5' ends. The relative strengths of the Le and TrC promoters for directing RNA synthesis were compared and found to be very similar. Thus, these findings highlighted a high degree of functional similarity between the RSV antigenomic and genomic promoters, but provided a further distinction between promoter requirements for transcription and replication.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892-0720, USA.
authors.forename: R
authors.initials: R
authors.lastname: Fearns
authors.forename: P L
authors.initials: PL
authors.lastname: Collins
authors.forename: M E
authors.initials: ME
authors.lastname: Peeples
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D018075:RNA, Complementary; D012367:RNA, Viral; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014779:Virus Replication
chemical_list: D020121:5' Untranslated Regions; D018075:RNA, Complementary; D012367:RNA, Viral