title: Diversity of cyanobacterial hydrogenases, a molecular approach.
abstract: In an effort to elucidate the diversity of cyanobacterial hydrogenases, we used a molecular approach. Filamentous strains from a broad range of sources were screened for the presence of hup (uptake hydrogenase), xisC (rearrangement within hupL), and hox (bidirectional hydrogenase) genes. As expected, an uptake hydrogenase seems to be present in all N(2)-fixing cyanobacteria. On the other hand, no evidence was found for the presence of a conventional bidirectional enzyme in several strains. Similarly, the presence of xisC is not a characteristic shared by all the heterocyst-forming cyanobacteria. Although tempting, it is not possible to establish a correlation between the presence/absence of the bidirectional hydrogenase and the occurrence of xisC. The natural molecular variation of hydrogenases in cyanobacteria is certainly a field to explore, both to understand the physiological functions of the respective enzymes and to identify a genetic background to be used when constructing a strain for photobiological H(2) production in a bioreactor.
authors.affiliation: Department of Physiological Botany, EBC, Uppsala University, Villavägen 6, S-752 36 Uppsala, Sweden. pmtamgn@gaia.ibmc.up.pt
authors.forename: P
authors.initials: P
authors.lastname: Tamagnini
authors.forename: J L
authors.initials: JL
authors.lastname: Costa
authors.forename: L
authors.initials: L
authors.lastname: Almeida
authors.forename: M J
authors.initials: MJ
authors.lastname: Oliveira
authors.forename: R
authors.initials: R
authors.lastname: Salema
authors.forename: P
authors.initials: P
authors.lastname: Lindblad
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics; D006864:Hydrogenase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D010088:Oxidoreductases; C021376:hydrogen dehydrogenase; C091789:HupL protein, Anabaena; D006864:Hydrogenase