Changes in benzodiazepine binding in a subkindling situation.
OBJECTIVE
A low dose of the benzodiazepine receptor inverse agonist methyl beta-carboline-3-carboxylate (beta-CCM) (1 mg/kg) was used to assess [3H]-flumazenil binding in a subkindling situation in Swiss mice.


METHODS
The brains were removed, and benzodiazepine receptor binding was studied every second day over 14 days of administration.


RESULTS
With each successive trial, Bmax values showed a steady and significant decrease, whereas Kd values showed a steady and significant increase. Behavioral data showed that at this low dose, actual kindling (seizuring) was not reached at the behavioral level.


CONCLUSIONS
The findings suggest that decreased gamma-aminobutyric acid (GABA) inhibition may occur even if behavioral effects of kindling are not observed.
Rössler
A S
AS
UMR 7593 CNRS "Personnalité et Conduites adaptatives," Faculté de Médecine Pitié-Salpêtrière, "Pathologie Expérimentale et Communications Cellulaires," Service de Biochimie, Hôpital Lariboisière, Paris, France.
Launay
J M
JM
Venault
P
P
Dodd
R H
RH
Chapouthier
G
G
D000818:Animals; D002243:Carbolines / Q000494:pharmacology*; D003292:Convulsants / Q000494:pharmacology*; D004195:Disease Models, Animal; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism; D005442:Flumazenil / Q000378:metabolism*; D018756:GABA Antagonists; D007696:Kindling, Neurologic / Q000187:drug effects*; D008297:Male; D051379:Mice; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism*; D012640:Seizures / Q000139:chemically induced* / Q000378:metabolism; D014316:Tritium
D002243:Carbolines; D003292:Convulsants; D018756:GABA Antagonists; D011963:Receptors, GABA-A; D014316:Tritium; D005442:Flumazenil; C036150:beta-carboline-3-carboxylic acid methyl ester