Induction of the ATP-sensitive potassium (uK(ATP)-1) channel by endotoxemia.
The effect of sepsis on the ubiquitously expressed ATP-sensitive potassium (uK(ATP)-1) channel expression was measured in Sprague-Dawley rat diaphragms. Rats were treated with either 0.5 ml saline or 20 mg/Kg E. coli lipopolysaccharides and sacrificed at 3, 6, 12, 24, or 48 h later. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that channel mRNA expression was increased at 3 h and continued to rise up to 48 h. Western blotting analysis showed a approximately 9-fold increase in channel protein expression 24 h after sepsis. Our results demonstrate that sepsis upregulates the uK(ATP)-1 channel.
Czaika
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G
Respiratory Muscle Biology Group, Centre de Recherche du CHUM, Hôpital Notre-Dame, Université de Montréal, Québec, Canada.
Gingras
Y
Y
Zhu
E
E
Comtois
A S
AS
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D003964:Diaphragm / Q000737:chemistry / Q000378:metabolism*; D019446:Endotoxemia / Q000139:chemically induced / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D015221:Potassium Channels / Q000032:analysis / Q000235:genetics*; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000502:physiology
D008070:Lipopolysaccharides; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger; D000255:Adenosine Triphosphate