Limitation of the AccuProbe Coccidioides immitis culture identification test: false-negative results with formaldehyde-killed cultures.
The AccuProbe Coccidioides immitis culture identification test (CI test) yielded false-negative results with formaldehyde-killed C. immitis submitted to a reference laboratory. Further evaluation with pure or mixed cultures or stored, heat-killed cultures revealed the CI test to be highly sensitive and specific for C. immitis except when the cultures were pretreated with formaldehyde.
Gromadzki
S G
SG
Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York 12201-2002, USA.
Chaturvedi
V
V
D003045:Coccidioides / Q000302:isolation & purification*; D005188:False Negative Reactions; D005404:Fixatives; D005557:Formaldehyde; D011933:Reagent Kits, Diagnostic
D005404:Fixatives; D011933:Reagent Kits, Diagnostic; D005557:Formaldehyde