Whole body and abdominal lipolytic sensitivity to epinephrine is suppressed in upper body obese women.
We measured whole body and regional lipolytic and adipose tissue blood flow (ATBF) sensitivity to epinephrine in 8 lean [body mass index (BMI): 21 +/- 1 kg/m(2)] and 10 upper body obese (UBO) women (BMI: 38 +/- 1 kg/m(2); waist circumference >100 cm). All subjects underwent a four-stage epinephrine infusion (0.00125, 0.005, 0.0125, and 0.025 microgram. kg fat-free mass(-1). min(-1)) plus pancreatic hormonal clamp. Whole body free fatty acid (FFA) and glycerol rates of appearance (R(a)) in plasma were determined by stable isotope tracer methodology. Abdominal and femoral subcutaneous adipose tissue lipolytic activity was determined by microdialysis and (133)Xe clearance methods. Basal whole body FFA R(a) and glycerol R(a) were both greater (P < 0.05) in obese (449 +/- 31 and 220 +/- 12 micromol/min, respectively) compared with lean subjects (323 +/- 44 and 167 +/- 21 micromol/min, respectively). Epinephrine infusion significantly increased FFA R(a) and glycerol R(a) in lean (71 +/- 21 and 122 +/- 52%, respectively; P < 0.05) but not obese subjects (7 +/- 6 and 39 +/- 10%, respectively; P = not significant). In addition, lipolytic and ATBF sensitivity to epinephrine was blunted in abdominal but not femoral subcutaneous adipose tissue of obese compared with lean subjects. We conclude that whole body lipolytic sensitivity to epinephrine is blunted in women with UBO because of decreased sensitivity in upper body but not lower body subcutaneous adipose tissue.
Horowitz
J F
JF
Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Klein
S
S
D000005:Abdomen; D000273:Adipose Tissue / Q000098:blood supply / Q000378:metabolism*; D000328:Adult; D001783:Blood Flow Velocity; D001824:Body Constitution; D015992:Body Mass Index; D004837:Epinephrine / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D015309:Glucose Clamp Technique; D005990:Glycerol / Q000097:blood; D006801:Humans; D007328:Insulin / Q000097:blood; D007700:Kinetics; D008066:Lipolysis / Q000187:drug effects*; D017551:Microdialysis; D009765:Obesity / Q000378:metabolism*; D010084:Oxidation-Reduction; D014980:Xenon Radioisotopes
D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014980:Xenon Radioisotopes; D005990:Glycerol; D004837:Epinephrine