title: IL-5 and eosinophils mediate the rejection of fully histoincompatible vascularized cardiac allografts: regulatory role of alloreactive CD8(+) T lymphocytes and IFN-gamma.
abstract: CD8(+) T lymphocytes are known to inhibit the development of eosinophilia and IL-5 synthesis in models of experimental lung disease. In transplantation, the rejection of fully mismatched cardiac allografts by recipients depleted of CD8(+) T cells is characterized by the recruitment of eosinophils in the rejected organs. We show here that this intragraft eosinophilia is dependent on the production of IL-5 since hearts transplanted into IL-5-deficient recipients depleted of CD8(+) cells did not contain eosinophils. More importantly, allograft survival was significantly extended in these animals. In mixed lymphocyte cultures (MLC), the presence of CD8(+) T cells in the responding cell population inhibited the secretion of IL-5. This inhibition was IFN-gamma dependent since adding neutralizing anti-IFN-gamma antibodies induced the production of IL-5. Furthermore, spleen cells isolated from IFN-gamma receptor (IFN-gammaR)-deficient mice secreted IL-5 upon allogeneic stimulation in primary MLC. In vivo, eosinophilia was observed in allografts rejected by IFN-gammaR-deficient recipients. On the contrary, grafts rejected by IFN-gammaR-deficient mice treated with neutralizing anti-IL-5 antibodies did not exhibit eosinophilic infiltration. Our study reveals the capacity of IL-5-secreting CD4(+) T cells and eosinophils to promote the rejection of heart allograft and demonstrates the importance of CD8(+) T cells and IFN-gamma in regulating this pathway of rejection.
authors.affiliation: Laboratory of Experimental Immunology, Université Libre de Bruxelles, Brussels, Belgium. mbraun@ulb.ac.be
authors.forename: M Y
authors.initials: MY
authors.lastname: Braun
authors.forename: F
authors.initials: F
authors.lastname: Desalle
authors.forename: A
authors.initials: A
authors.lastname: Le Moine
authors.forename: M
authors.initials: M
authors.lastname: Pretolani
authors.forename: P
authors.initials: P
authors.lastname: Matthys
authors.forename: R
authors.initials: R
authors.lastname: Kiss
authors.forename: M
authors.initials: M
authors.lastname: Goldman
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D016027:Heart Transplantation; D006650:Histocompatibility Testing; D007371:Interferon-gamma / Q000276:immunology*; D015848:Interleukin-5 / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
chemical_list: D015848:Interleukin-5; D007519:Isoantigens; D007371:Interferon-gamma