Catalytic mechanism of an abzyme displaying a beta-lactamase-like activity.
A catalytic IgG (Ab2) displaying a beta-lactamase-like activity was previously obtained by using the antiidiotypic pathway: the particularity of this antibody is that it is a true antiidiotype of the beta-lactamase active site. We have previously demonstrated that this IgG has retained some of the structural information displayed by the beta-lactamase active site, evident from data that polyclonal anti-Ab2 antibodies (Ab3) recognize beta-lactamase. In this article, we investigated the catalytic mechanism of the abzyme compared to that of the enzyme. The experimental data allow us to hypothesize the catalytic residues required for catalysis.
Avalle
B
B
UPRES A 6022 Génie Enzymatique et Cellulaire, Université de Technologie de Compiègne, France. avalle@utc.fr
Débat
H
H
Friboulet
A
A
Thomas
D
D
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D017151:Antibodies, Catalytic / Q000378:metabolism*; D001409:Bacillus cereus / Q000201:enzymology; D020134:Catalytic Domain; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D001618:beta-Lactamases / Q000276:immunology* / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; D017151:Antibodies, Catalytic; D007074:Immunoglobulin G; D001618:beta-Lactamases