title: Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp.
abstract: Transfer of VanB-type resistance to glycopeptides among enterococci has been reported to be associated with the movement of large chromosomal genetic elements or of plasmids. The authors report the characterization of the 34 kb transposon Tn1549 borne by a plasmid related to pAD1 and conferring vancomycin resistance in clinical isolates of Enterococcus spp. Tn1549 contained 30 ORFs and appeared to be organized like the Tn916 family of conjugative transposons into three functional regions: (i) the right end, implicated in the excision-integration process; (ii) the central part, in which the vanB2 operon replaces the tet(M) gene; and (iii) the left extremity, in which eight of the 18 ORFs could be implicated in the conjugative transfer.
authors.affiliation: Unité des Agents Antibactériens1 and Laboratoire de Génomique des Micro-organismes Pathogènes2, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Garnier
authors.affiliation: Unité des Agents Antibactériens1 and Laboratoire de Génomique des Micro-organismes Pathogènes2, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Sead
authors.initials: S
authors.lastname: Taourit
authors.affiliation: Unité des Agents Antibactériens1 and Laboratoire de Génomique des Micro-organismes Pathogènes2, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Glaser
authors.affiliation: Unité des Agents Antibactériens1 and Laboratoire de Génomique des Micro-organismes Pathogènes2, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Courvalin
authors.affiliation: Unité des Agents Antibactériens1 and Laboratoire de Génomique des Micro-organismes Pathogènes2, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Galimand
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003227:Conjugation, Genetic; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon; D010957:Plasmids / Q000235:genetics; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004251:DNA Transposable Elements; C079913:VanB protein, Enterococcus