Effects of a muramidase on a mixed bacterial community.
In bacterial communities one bacterium can influence the growth of other members of the population. These interactions may be based on nutritional factors or may occur via bacterial signaling molecules that are released in the medium. We present an example, showing that in addition to the above means of interactions, muramidases, enzymes that specifically cleave peptidoglycan chains, can also mediate interactions between bacteria. Using fluorescent in situ hybridization we demonstrate that Lactococcus lactis muramidase AcmA can hydrolyze the cell wall of Streptococcus thermophilus, without affecting viability. This intercellular activity of the lactococcal muramidase results in chain disruption of streptococci in vivo. Our data lead us to propose that chains can give growth advantages to streptococci in aerobic conditions.
Mercier
C
C
Unité de Recherches Laitières et Génétique Appliquée, Domaine de Vilvert, 78352, INRA, Jouy-en-Josas, France. 7
Domakova
E
E
Tremblay
J
J
Kulakauskas
S
S
D000332:Aerobiosis; D001426:Bacterial Proteins / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D017404:In Situ Hybridization, Fluorescence; D013294:Lactococcus lactis / Q000201:enzymology*; D009113:Muramidase / Q000378:metabolism*; D010457:Peptidoglycan / Q000737:chemistry / Q000378:metabolism; D013291:Streptococcus / Q000737:chemistry / Q000254:growth & development*
D001426:Bacterial Proteins; D003470:Culture Media; D010457:Peptidoglycan; C092497:AcmA protein, Lactococcus lactis; D009113:Muramidase