title: Correlation of genetic polymorphism of cytochrome P4502D6 with dextromethorphan oxidative metabolism in Chinese.
abstract: OBJECTIVE
  To determine the genetic mechanism of dextromethorphan oxidative polymorphism in Chinese subjects.
  
  
  METHODS
  CYP2D6 genotype was analyzed by polymerase chain reaction (PCR) and Hph I restriction fragment length polymorphism (RFLP) techniques in 119 healthy Chinese subjects.
  
  
  RESULTS
  The gene frequency of CYP2D6*10B was 58.4%, including 13(10.9%) of homozygous wild type(w/w), 33 (27.7%) of homozygous mutant(m/m), and 73(61.3%) of heterozygous genotypes(m/w). Twenty-nine subjects out of 42 intermediate metabolizers(70%) of dextromethorphan were homozygous for CYP2D6*10B. One poor metabolizer subject also showed an m/m genotype. Besides, 10 Chinese subjects were tested and excluded for the presence of any of the six mutant alleles associated with poor metabolism of CYP2D6 in Caucasians.
  
  
  CONCLUSIONS
  The CYP2D6*10B allele containing the C(188)--> T mutation is the major cause of CYP2D6 polymorphism in relation to diminished dextromethorphan oxidative capacity in Chinese subjects.
authors.affiliation: Department of Clinical Pharmacology, Nanjing General Hospital of Nanjing Command, Nanjing, Jiangsu, 210002 P. R. China. wmcai@public1. ptt.js.cn
authors.forename: W
authors.initials: W
authors.lastname: Cai
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Tao
authors.forename: S
authors.initials: S
authors.lastname: Ling
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics*; D003915:Dextromethorphan / Q000378:metabolism*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D011110:Polymorphism, Genetic
chemical_list: D003915:Dextromethorphan; D019389:Cytochrome P-450 CYP2D6