Aut7p, a soluble autophagic factor, participates in multiple membrane trafficking processes.
Aut7p, a protein recently implicated in autophagic events in the yeast Saccharomyces cerevisiae, exhibits significant homology to a mammalian protein, p16, herein termed GATE-16 (Golgi-associated ATPase Enhancer of 16 kDa), a novel intra-Golgi transport factor. Here we provide evidence for the involvement of Aut7p in different membrane trafficking processes. Aut7p largely substitutes for the activity of GATE-16 in mammalian intra-Golgi transport in vitro. In vivo, AUT7 interacts genetically with endoplasmic reticulum to Golgi SNAREs, specifically with BET1 and SEC22. Aut7p interacts physically with the following two v-SNAREs: Bet1p, which is involved in endoplasmic reticulum to Golgi vesicular transport, and Nyv1p, implicated in vacuolar inheritance. We suggest that, in addition to its role in autophagocytosis, Aut7p has pleiotropic effects and participates in at least two membrane traffic events.
Legesse-Miller
A
A
Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, 76100 Israel.
Sagiv
Y
Y
Glozman
R
R
Elazar
Z
Z
D000818:Animals; D000071190:Autophagy-Related Protein 8 Family; D001921:Brain / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000502:physiology; D005656:Fungal Proteins / Q000378:metabolism; D005838:Genotype; D006056:Golgi Apparatus / Q000502:physiology; D007425:Intracellular Membranes / Q000502:physiology; D008322:Mammals; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016366:Open Reading Frames; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins
C113757:ATG8 protein, S cerevisiae; D000071190:Autophagy-Related Protein 8 Family; D002352:Carrier Proteins; D005656:Fungal Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins