title: Response to nitrogen starvation in Corynebacterium glutamicum.
abstract: Proteins strongly synthesized in Corynebacterium glutamicum during nitrogen restriction were examined by two-dimensional gel electrophoresis and microsequencing. Two main groups of enzymes were identified beside miscellaneous proteins, enzymes involved (i) in protein synthesis, and (ii) in carbon metabolism. Biochemical measurements revealed an increase of oxygen consumption during nitrogen starvation, indicating an enhanced energy demand of the cells. By Northern hybridizations, an increased transcription for the gap and fda genes upon nitrogen deprivation was shown.
authors.affiliation: ABteilung Mikrobiologie, Universität Osnabrück, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Schmi
authors.forename: E M
authors.initials: EM
authors.lastname: Uhlemann
authors.forename: L
authors.initials: L
authors.lastname: Nolden
authors.forename: G
authors.initials: G
authors.lastname: Wersch
authors.forename: R
authors.initials: R
authors.lastname: Hecker
authors.forename: T
authors.initials: T
authors.lastname: Hermann
authors.forename: A
authors.initials: A
authors.lastname: Marx
authors.forename: A
authors.initials: A
authors.lastname: Burkovski
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D015152:Blotting, Northern; D003352:Corynebacterium / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D009584:Nitrogen; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D012329:RNA, Bacterial; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005634:Fructose-Bisphosphate Aldolase; D009584:Nitrogen