Chaetoatrosin A, a novel chitin synthase II inhibitor produced by Chaetomium atrobrunneum F449.
Chaetoatrosin A, a novel chitin synthase II inhibitor, was isolated from the culture broth of fungus F449, which was identified as Chaetomium atrobrunneum F449. Chaetoatrosin A was purified by solvent partition, silica gel, ODS, preparative TLC, and Sephadex LH-20 column chromatographies, consecutively. The structure of chaetoatrosin A was assigned as 1,8-dihydroxy-3(2-hydroxypropionyl)-6-methoxynaphthalene on the basis of various spectroscopic analyses including UV, IR, mass spectral, and NMR. Its molecular weight and formula were found to be 262 and C14H14O5, respectively. ,Chaetoatrosin A inhibited chitin synthase II by 50% at the concentration of 104 microg/ml in an enzyme assay system. This compound showed antifungal activities against Rhizoctonia solani, Pyricularia oryzae, Botrytis cinerea, Cryptococcus neoformans and Trichophyton mentagrophytes.
Hwang
E I
EI
Antibiotics Research Lab, Korea Research Institute of Bioscience and Biotechnology, Yusung, Taejon, Korea.
Yun
B S
BS
Kim
Y K
YK
Kwon
B M
BM
Kim
H G
HG
Lee
H B
HB
Bae
K S
KS
Kim
S U
SU
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D002597:Chaetomium / Q000145:classification / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D002687:Chitin Synthase / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005285:Fermentation; D005658:Fungi / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009181:Mycoses / Q000382:microbiology; D009284:Naphthols / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D009284:Naphthols; C409193:chaetoatrosin A; D002687:Chitin Synthase