title: Cytolytic T lymphocytes raised against a human bladder carcinoma recognize an antigen encoded by gene MAGE-A12.
abstract: Human bladder carcinoma line LB831-BLC expresses several distinct Ags that are recognized by different autologous CTL. Here, we show that one of these Ags is presented by HLA-Cw7 and encoded by gene MAGE-A12. This is the first time that CTL directed against a MAGE-encoded Ag have been derived from the lymphocytes of a patient with cancer other than melanoma. This new Ag was found to be nonapeptide VRIGHLYIL, corresponding to position 170-178 of the MAGE-A12 protein. Gene MAGE-A12 is silent in normal tissues except in male germline cells, which do not express HLA molecules. It is expressed in 26-62% of melanomas, infiltrating bladder carcinomas, lung carcinomas, esophageal carcinomas, and head and neck carcinomas. Because HLA-Cw7 is present in 43% of Caucasians, this new Ag is shared by many tumors and should be a useful target for cancer immunotherapy.
authors.affiliation: Ludwig Institute for Cancer Research, Brussels Branch, and Cellular Genetics Unit, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Heidecker
authors.forename: F
authors.initials: F
authors.lastname: Brasseur
authors.forename: M
authors.initials: M
authors.lastname: Probst-Kepper
authors.forename: M
authors.initials: M
authors.lastname: Guéguen
authors.forename: T
authors.initials: T
authors.lastname: Boon
authors.forename: B J
authors.initials: BJ
authors.lastname: Van den Eynde
mesh_terms: D000368:Aged; D017951:Antigen Presentation / Q000235:genetics; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D002999:Clone Cells; D004938:Esophageal Neoplasms / Q000235:genetics; D052138:Genes, Neoplasm / Q000276:immunology*; D006258:Head and Neck Neoplasms / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D008297:Male; D008545:Melanoma / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; C409589:MAGEA12 protein, human; D009363:Neoplasm Proteins; D010446:Peptide Fragments