GFP-centrin as a marker for centriole dynamics in living cells.
A long-standing puzzle in cell biology is the question of how cells generate one and only one new centrosome in each cell cycle and what is the role of the centriole pair in this process. In this study, the introduction of GFP-centrin into cultured cells allows direct visualization of centriole behavior in living cells and in real time. Using this method, centriole dynamics can be observed throughout the cell cycle and following a variety of experimental treatments. Our studies demonstrate that the biogenesis of new centrioles from individual members of a preexisting centriole pair is asynchronous: the older centriole initiates assembly of a new daughter centriole before the younger centriole initiates assembly of its daughter.
White
R A
RA
Tumor Biology Program, Mayo Clinic, Rochester, MN 55905, USA.
Pan
Z
Z
Salisbury
J L
JL
D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D002453:Cell Cycle; D002478:Cells, Cultured; D002502:Centrioles / Q000737:chemistry / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection
D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D008164:Luminescent Proteins; D011994:Recombinant Proteins; C056608:caltractin; D049452:Green Fluorescent Proteins