A new model of allergic rhinitis in rats by topical sensitization and evaluation of H(1)-receptor antagonists.
An animal model of chronic allergic rhinitis was developed by repeated local booster sensitization into the nasal cavity in sensitized rats. The severity of allergic rhinitis was assessed by determining the extent of two markers of nasal allergic symptoms (sneezing and nasal rubbing) after antigen challenge. The number of incidents of sneezing and nasal rubbing was markedly increased during intranasal instillation of antigen in sensitized rats. The PCA titers were also markedly elevated by intranasal sensitization. Some histamine H(1)-receptor antagonists such as chlorpheniramine, ketotifen, astemizole and epinastine inhibited the increase in antigen-induced nasal symptoms in a dose-related manner. Nasal rubbing was more potently inhibited by H(1)-receptor antagonists than sneezing. In conclusion, we developed a chronic allergic rhinitis model showing nasal symptoms in rats, and this model may be useful for evaluating the effects of drugs on allergic rhinitis.
Sugimoto
Y
Y
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-naka 1-1-1, 700-8530, Okayama, Japan.
Kawamoto
E
E
Chen
Z
Z
Kamei
C
C
D000485:Allergens; D000818:Animals; D018926:Anti-Allergic Agents / Q000627:therapeutic use*; D016589:Astemizole / Q000627:therapeutic use; D002744:Chlorpheniramine / Q000627:therapeutic use; D003984:Dibenzazepines / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006634:Histamine H1 Antagonists / Q000627:therapeutic use*; D007093:Imidazoles / Q000627:therapeutic use; D007114:Immunization; D007665:Ketotifen / Q000627:therapeutic use; D009296:Nasal Cavity / Q000187:drug effects / Q000276:immunology; D010047:Ovalbumin; D010323:Passive Cutaneous Anaphylaxis; D051381:Rats; D017208:Rats, Wistar; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology; D012912:Sneezing / Q000187:drug effects; D013997:Time Factors
D000485:Allergens; D018926:Anti-Allergic Agents; D003984:Dibenzazepines; D006634:Histamine H1 Antagonists; D007093:Imidazoles; D002744:Chlorpheniramine; D016589:Astemizole; D010047:Ovalbumin; C053090:epinastine; D007665:Ketotifen