Gps mutations in Chilean patients harboring growth hormone-secreting pituitary tumors.
Hypersecretion of GH is usually caused by a pituitary adenoma and about 40% of these tumors exhibit missense gsp mutations in Arg201 or Gln227 of the Gs, gene. We studied 20 pituitary tumors obtained from patients with GH hypersecretion. One tumor was resected from an 11 year-old boy with a 3 year history of accelerated growth, associated with increased concentrations of serum GH and IGF-I, which were not suppressed by glucose administration. The remaining 19 tumors were obtained from adult acromegalic patients, who had elevated baseline serum GH levels that did not show evidence of suppression after administration of glucose. The gsp mutations were studied by enzymatic digestion of the amplified PCR fragment of exon 8 (Arg201) and exon 9 (Gln227) with the enzymes NlaIII and NgoAIV, respectively. The tumors obtained from the boy and from nine of the 19 patients with acromegaly exhibited the gsp mutation R201H. None of the tumors had the Gln227 mutation. The gsp positive patients tended to be older, had smaller tumors, and had preoperative basal serum GH levels which were significantly lower (21 +/- 6 vs 56 +/- 16 microg/l, p<0.05) than the gsp negative patients. In this study, we documented the presence of a gsp mutation in Arg201 in a boy with gigantism and in approximately half of 19 Chilean adult patients with acromegaly, similar to other populations.
Johnson
M C
MC
Institute of Maternal and Child Research, University of Chile School of Medicine, Santiago.
Codner
E
E
Eggers
M
M
Mosso
L
L
Rodriguez
J A
JA
Cassorla
F
F
D000236:Adenoma / Q000150:complications / Q000235:genetics* / Q000378:metabolism*; D002648:Child; D002677:Chile; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000235:genetics*; D005877:Gigantism / Q000209:etiology / Q000235:genetics / Q000503:physiopathology; D005951:Glucose Tolerance Test; D006128:Growth / Q000502:physiology; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging; D008297:Male; D020125:Mutation, Missense / Q000235:genetics*; D015513:Oncogene Proteins / Q000235:genetics*; D010911:Pituitary Neoplasms / Q000150:complications / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003062:Codon; D015513:Oncogene Proteins; D019382:Human Growth Hormone; D004247:DNA; D019205:GTP-Binding Protein alpha Subunits, Gs