Identification of morphine in the rat adrenal gland.
Morphine was identified in rat adrenal extracts by reverse-phase HPLC, following liquid and solid extraction. All experiments were carefully performed to prevent exogenous morphine contamination. The morphine extracted from adrenal tissue (105.31 ng/g of wet adrenal gland) was identical to that of a morphine internal standard. The morphine corresponding HPLC fractions were further analyzed by gas chromatography-mass spectrometry and found to be identical to synthetic morphine. The study conclusively proves morphine is endogenous to the rat adrenal gland.
Goumon
Y
Y
Neuroscience Research Institute, State University of New York, College at Old Westbury, Old Westbury, NY 11568, USA.
Stefano
G B
GB
D000311:Adrenal Glands / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D009020:Morphine / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009020:Morphine