Detection of progesterone receptor transcript in human spermatozoa.
The present study, to our knowledge, is the first to demonstrate presence of progesterone receptor (PR) transcript in human spermatozoa. The study shows the presence of low copy number PR mRNA in mature human spermatozoa. The PR transcript in spermatozoa was detected by reverse transcriptase-polymerase chain reaction using primers specific for the hormone binding domain and the DNA binding domain of the conventional uterine PR. Further, the cDNA sequence of the partial PR transcript from spermatozoa was found to be identical to the region spanning nucleotides 2694 to 3230 of the conventional PR full-length cDNA sequence. This study also indirectly suggests that the PR protein indeed is an intrinsic sperm protein and is not acquired through proteinaceous secretions of accessory reproductive organs.
Sachdeva
G
G
Institute for Research in Reproduction, Indian Council of Medical Research, Parel, Mumbai 400 012, India.
Shah
C A
CA
Kholkute
S D
SD
Puri
C P
CP
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011374:Progesterone / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D011980:Receptors, Progesterone / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000737:chemistry*
D012333:RNA, Messenger; D011980:Receptors, Progesterone; D011374:Progesterone; D004247:DNA