Influence of ring opening-closure equilibrium of oxanine, a novel damaged nucleobase, on migration behavior in capillary electrophoresis.
Oxanine (Oxa) is a novel damaged nucleobase which is generated from guanine by HNO2 or NO. As a fundamental study for detection of Oxa formed in vivo, capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) have been tested by changing the pH of the running buffer. At pH 7, CZE did not separate Oxa from seven other nucleobases, and MEKC separated Oxa but their peaks migrated close together. In both the techniques, an extreme peak broadening occurred for Oxa around pH 9 and a good peak separation was achieved at pH 12. The behavior of the Oxa peak is discussed in relation to the unique multistep acid-base equilibria of Oxa.
Suzuki
T
T
Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Japan.
Yamada
M
M
Ide
H
H
Kanaori
K
K
Tajima
K
K
Morii
T
T
Makino
K
K
D002021:Buffers; D004249:DNA Damage; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D012263:Ribonucleosides / Q000737:chemistry
D002021:Buffers; D012263:Ribonucleosides; C032886:oxanosine