Effects of spinach powder fat-soluble extract on proliferation of human gastric adenocarcinoma cells.
Four kinds of assays were used to study the effect of a fat-soluble extract of spinach powder (SPFE) on the proliferation of human gastric adenocarcinoma cell line (SGC-7901) in vitro. These studies included: (i) cell growth assay, (ii) colony forming assay, (iii) MTT colorimetric assay, and (iv) 3H-TdR incorporation assay. The concentrations of SPFE expressed as the level of beta-carotene in the medium were 2 x 10(-8), 2 x 10(-7) and 2 x 10(-6) mol/L beta-carotene in assay (i)-(iii), but 4 x 10(-8), 4 x 10(-7) and 4 x 10(-6) mol/L beta-carotene in assay (iv) respectively. The results indicated that SPFE inhibited the proliferation and colony forming ability of SGC-7901 cells. And in MTT assay, SPFE inhibited the viability of SGC-7901 cells, but no inhibitory effect of SPFE was observed on the viability of lymphocytes in peripheral blood of healthy people. Finally, in the 3H-TdR incorporation test, both SPFE and beta-carotene showed significant inhibitory effects on DNA synthesis in SGC-7901 cells, but SPFE was more effective than beta-carotene.
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Department of Nutrition and Food Hygiene, School of Public Health, West China University of Medical Sciences (WCUMS), Chengdu, China.
Huang
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Chen
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Hou
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YH
D000230:Adenocarcinoma / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology; D012995:Solubility; D018724:Spinacia oleracea; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D010936:Plant Extracts