Monitoring the irradiation-induced conformational changes of ovalbumin by using monoclonal antibodies and surface plasmon resonance.
Two types of conformationally specific anti-irradiated ovalbumin monoclonal antibodies were prepared in order to study and monitor irradiation-induced structural changes in the ovalbumin molecule. Surface plasmon resonance (SPR) detection was used to investigate the kinetic parameters of the reaction between antibodies and ovalbumin which had been administered with different doses of irradiation (0, 1.5, 2.0, 5.0, 10, 20, 50, and 100 kGy). The results demonstrate that the combination of monoclonal antibodies and the SPR method can be used to characterize the irradiation-induced conformational change with an unlabelled reagent.
Masuda
T
T
Research Institute for Food Science, Kyoto University Gokasho, Uji, Japan. masuda@food2.food.kyoto-u.ac.jp
Yasumoto
K
K
Kitabatake
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007700:Kinetics; D051379:Mice; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology / Q000528:radiation effects*; D011487:Protein Conformation / Q000528:radiation effects; D020349:Surface Plasmon Resonance / Q000379:methods
D000911:Antibodies, Monoclonal; D010047:Ovalbumin