tef1, a Phytophthora infestans gene encoding translation elongation factor 1alpha.
From a set of Phytophthora infestans cDNA clones randomly selected from a potato-P. infestans interaction cDNA library, three out of 22 appeared to correspond to a gene encoding translation elongation factor 1alpha. The gene, called tef1, is a single copy gene in P. infestans. During the life cycle of P. infestans, tef1 is expressed in all developmental stages. Alignment and phylogeny analysis based on EF-1alpha proteins from several taxonomic groups, including fungi, slime molds, algae, higher plants and archeabacteria, support the view that oomycetes evolved completely independently from the true fungi. In the phylogenetic tree, P. infestans EF-1alpha forms one branch with EF-1alpha from the unicellular alga Cyanophora paradoxa, an organism belonging to a taxonomic group that occupies a key position in the evolution of plastids.
van't Klooster
J W
JW
Laboratory of Phytopathology, Wageningen University and Graduate School of Experimental Plant Sciences, Netherlands.
van den Berg-Velthuis
G
G
van West
P
P
Govers
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010802:Phylogeny; D010838:Phytophthora / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004271:DNA, Fungal; D020648:Peptide Elongation Factor 1; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; C410826:TEF1 protein, S cerevisiae