A precerebellin-like protein is part of the acute phase response in rainbow trout, Oncorhynchus mykiss.
The acute phase response (APR) has a long evolutionary history, but it remains to be characterized fully in lower vertebrates. To study the acute phase proteins of a teleost, rainbow trout (Oncorhynchus mykiss), we induced an APR by injecting Vibrio bacterin emulsified in FIA. In samples taken over the next 3 weeks, the total plasma protein profile changed consistently as seen in one and two-dimensional SDS PAGE. One 18.1 kD upregulated protein was isolated from 2D gels and an N-terminal sequence obtained. Using reverse transcriptase-PCR, a 700 bp cDNA sequence was amplified. The sequence is 53% similar at the amino acid level with rat precerebellin (regions aa 42-184 from trout and aa 89-224 precerebellin), and 46% similar with the globular portion of the human B chain of the first complement component C1q. However, it lacks the collagen portion of C1q with its characteristic Gly-X-Y repeats. The isolated protein seems to be involved in the inflammatory response but its physiological function is unknown.
Gerwick
L
L
Department of Zoology and Environmental Health Sciences Center, Oregon State University, OR, Corvallis 97331, USA. gerwickl@bcc.orst.edu
Reynolds
W S
WS
Bayne
C J
CJ
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000210:Acute-Phase Reaction / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011498:Protein Precursors / Q000096:biosynthesis*; D051381:Rats; D017386:Sequence Homology, Amino Acid
D000209:Acute-Phase Proteins; D009419:Nerve Tissue Proteins; D011498:Protein Precursors