Electrically mediated plasmid DNA delivery to hepatocellular carcinomas in vivo.
Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. In vivo electroporation has been used to enhance delivery of chemotherapeutic agents to tumors in both animal and human studies. Recently, this delivery technique has been extended to large molecules such as plasmid DNA. Here, the successful delivery of plasmids encoding reporter genes to rat hepatocellular carcinomas by in vivo electroporation is demonstrated.
Heller
L
L
Center for Molecular Delivery, University of South Florida, Tampa 33612, USA.
Jaroszeski
M J
MJ
Coppola
D
D
Pottinger
C
C
Gilbert
R
R
Heller
R
R
D000818:Animals; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D008297:Male; D010957:Plasmids / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley