title: Somatostatin and somatostatin receptors in the pig pineal gland during postnatal development: an immunocytochemical study.
abstract: An immunohistochemical study of the pineal gland of the domestic pig was carried out using rabbit antisera raised against synthetic peptide fragments corresponding to different amino acid sequences of the prosomatostatin, the somatostatin-14, and the somatostatin-28 molecule. The study was supplemented by immunohistochemical staining with rabbit antisera raised against five subtypes of somatostatin receptors. The pineal glands were taken from the newborn, 21-day-old and 7-month-old pigs. Immunoreactive nerve fibers and cells were observed in the pineal gland with all the antisera against somatostatin and prosomatostatin. The nerve fibers were located throughout the pineal gland-in the capsule, connective septa, and parenchyma-with the highest density in proximo-ventral part of the gland. The somatostatin positive fibers were also found in the habenular and posterior commissurae areas. Somatostatin-immunoreactive cell bodies were observed mostly in the central part of the gland. These results point to the existence of two somatostatin sources in the pig pineal gland: 1) nerve fibers, probably of central origin; and 2) cells that may represent intrapineal neurons or specialised pinealocytes. A clear difference in the immunoreactivity between newborn, 21-day-old, and 7-month-old pigs was found. Generally, the density of nerve fibers was lower in adult than young animals. The number of the cells also decreased with age. By using the antisera against the five somatostatin receptors, only sst3 - receptor immunoreactivity could be detected. The receptor-immunoreactivity was confined to varicose and smooth fibers and some cells. The sst(3)-receptor positive structures were localised in all parts of the gland and their number was higher in younger pigs.
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, Warmia-Masurian University, 10-713 Olsztyn, Poland. przybyl@moskit.art.olsztyn.pl
authors.forename: B
authors.initials: B
authors.lastname: Przybylska-Gornowicz
authors.forename: L
authors.initials: L
authors.lastname: Helboe
authors.forename: B
authors.initials: B
authors.lastname: Lewczuk
authors.forename: M
authors.initials: M
authors.lastname: Møller
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000378:metabolism; D010870:Pineal Gland / Q000254:growth & development / Q000378:metabolism*; D011498:Protein Precursors / Q000032:analysis*; D017481:Receptors, Somatostatin / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis*; D013552:Swine / Q000254:growth & development / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D017481:Receptors, Somatostatin; C101130:somatostatin receptor type 1; D013004:Somatostatin; C028456:prosomatostatin