Detection of human and animal rotavirus sequences in drinking water.
Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplified fragments-three of them of animal origin (porcine or bovine) and one of human origin.
Gratacap-Cavallier
B
B
Laboratoire de Virologie, Centre Hospitalier Universitaire, 38043 Grenoble, Faculté de Médecine, 38000 Grenoble, France. BGratacap@chu-grenoble.fr
Genoulaz
O
O
Brengel-Pesce
K
K
Soule
H
H
Innocenti-Francillard
P
P
Bost
M
M
Gofti
L
L
Zmirou
D
D
Seigneurin
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D036022:Capsid Proteins; D002417:Cattle; D002648:Child; D004326:Drinking; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D013552:Swine; D014871:Water Microbiology; D014881:Water Supply
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus