Changes in [3H]citalopram binding in cerebral cortex after antidepressant treatment in monoamine-impaired rats.
The [3H]citalopram binding after three weeks vehicle, desipramine 10 mg/kg or citalopram 5 mg/kg treatment was studied in the cerebral cortex of normal, DSP-4-, and p-CPA-impaired rats. The DSP-4 50 mg/kg treatment decreased the affinity (Kd), but increased the maximal number of the apparent binding sites (Bmax) of the 5-hydroxytryptamine transporter (5-HTT). This effect was reversed by desipramine 10 mg/kg treatment. The p-CPA 350 mg/kg treatment decreased the Bmax value while the antidepressant treatment did not influence this parameter. In conclusion, our experiments demonstrate that the monoaminergic impairment induced by DSP-4 and p-CPA treatment evokes opposite changes in the 5-HTT binding characteristics and these changes are partially reversed by the chronic antidepressant treatment.
Pruus
K
K
Department of Pharmacology, University of Tartu, Estonia.
Rudissaar
R
R
Skrebuhhova
T
T
Allikmets
L
L
Matto
V
V
D000818:Animals; D000929:Antidepressive Agents, Tricyclic / Q000494:pharmacology; D001596:Benzylamines / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D015283:Citalopram / Q000493:pharmacokinetics*; D003891:Desipramine / Q000494:pharmacology; D010134:Fenclonine / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018490:Serotonin Agents / Q000494:pharmacology; D017367:Selective Serotonin Reuptake Inhibitors / Q000493:pharmacokinetics*; D013565:Sympatholytics / Q000494:pharmacology
D000929:Antidepressive Agents, Tricyclic; D001596:Benzylamines; D015283:Citalopram; D003891:Desipramine; D010134:Fenclonine; D018490:Serotonin Agents; D017367:Selective Serotonin Reuptake Inhibitors; D013565:Sympatholytics; C012102:DSP 4