Expression and purification of woodchuck tumour necrosis factor alpha.
The production of recombinant woodchuck cytokines is an essential prerequisite to study the immune response to hepadnavirus infection in the woodchuck model. Woodchuck tumour necrosis factor-alpha (TNF-alpha) was expressed in mammalian cells and in Escherichia coli. A test system for the biological activity of woodchuck TNF-alpha was established on basis of its cytotoxic effect to the murine fibrosarcoma cell line L929. Recombinant TNF-alpha was purified and used for the production of neutralizing antisera.
Lohrengel
B
B
Institut für Virologie, Universitätsklinikum Essen, Hufelandstr. 55, Essen, 45122, Germany.
Lu
M
M
Bauer
D
D
Roggendorf
M
M
D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004926:Escherichia coli; D005354:Fibrosarcoma; D007074:Immunoglobulin G; D007739:L Cells; D008392:Marmota; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D015183:Restriction Mapping; D014162:Transfection; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000302:isolation & purification* / Q000494:pharmacology
D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha