Unravelling genetic data by arrayed primer extension.
We have developed a method for arrayed primer extension (APEX) on an oligonucleotide microchip together with the 4-color fluoresence imaging equipment and supporting software, that allows analysis of the DNA sequence and changes in it. Mutation analysis of BRCA1 gene and single nucleotide polymorphism (SNP) chip for genotyping were used as a model system. Chip surface chemistry, template preparation and APEX reaction conditions were optimised and the assay is ready to be implemented in variety of DNA analysis from SNP testing to DNA resequencing.
Tõnisson
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N
Institute of Molecular and Cell Biology, University of Tartu, Estonian Biocentre.
Kurg
A
A
Kaasik
K
K
Lõhmussaar
E
E
Metspalu
A
A
D000483:Alleles; D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D005260:Female; D019398:Genes, BRCA1; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods; D020641:Polymorphism, Single Nucleotide
D017931:DNA Primers