title: Effect of linoleic acid-albumin on post-thaw survival of in vitro-produced bovine embryos at the 16-cell stage.
abstract: The effect of addition of linoleic acid-albumin (LAA) to culture medium before freezing on the survival rate of bovine 16-cell embryos after freezing-thawing was investigated. Embryos were incubated in CR1aa containing LAA (0.25 mg/ml) for 4 days after insemination. A conventional slow cooling method was used, in which embryos were cooled at a rate of 0.3 degrees C/min to -30 degrees C in medium supplemented with 1.5 M ethylene glycol and 0.2 M trehalose. The developmental rate to the blastocyst stage of thawed embryos that had been cultured with LAA-containing medium before freezing was higher than that of these cultured without LAA (P<0.05). However, with fresh, non-frozen, embryos that were incubated under the same culture conditions (with and without LAA), no such difference was found.
authors.affiliation: Hyogo Prefectural Agricultural Institute, Kasai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tominaga
authors.forename: Y
authors.initials: Y
authors.lastname: Hamada
authors.forename: T
authors.initials: T
authors.lastname: Yabuue
authors.forename: T
authors.initials: T
authors.lastname: Ariyoshi
mesh_terms: D000418:Albumins; D000818:Animals; D002417:Cattle / Q000196:embryology*; D015925:Cryopreservation / Q000662:veterinary*; D003470:Culture Media; D004624:Embryo Transfer / Q000662:veterinary; D019787:Linoleic Acid / Q000494:pharmacology*
chemical_list: D000418:Albumins; D003470:Culture Media; D019787:Linoleic Acid