title: c-Myc expression in human anagen hair follicles.
abstract: The hair follicle represents a very attractive organ system for studying the precise balance between cell proliferation, growth, differentiation, and death of cells, because it periodically and regularly regenerates, retaining its morphogenetic signals throughout its life. One of the most intriguing oncogenes which is able to induce both cell growth and apoptosis, depending upon the environmental conditions, is c-myc. The aim of the present study was to investigate its presence and localization in human hair follicles by immunohistochemistry and immunofluorescence. Our observations demonstrated the consistent presence of two clusters of c-Myc-expressing cells in anagen follicles, located in two annular regions of the inner root sheath, at the border between cells characterized by putative trichohyalin granules and cells which are keratinized. The lower group belongs to Henle's layer, while the upper group belongs to Huxley's layer. c-Myc oncoprotein seems to favour apoptosis/differentiation and may be a marker for terminal differentiation of trichocytes, at least in the inner root sheath. Our findings agree with the interpretation that the complex morphology of the hair follicle reflects its complex function; the extrusion of a highly organized multicellular structure, the hair shaft, driven by another highly organized multicellular structure, the inner root sheath.
authors.affiliation: Institute of Human Anatomy, University of Milan, Via Mangiagalli 31, 20133 Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Rumio
authors.forename: E
authors.initials: E
authors.lastname: Donetti
authors.forename: A
authors.initials: A
authors.lastname: Imberti
authors.forename: I
authors.initials: I
authors.lastname: Barajon
authors.forename: E
authors.initials: E
authors.lastname: Prosperi
authors.forename: M F
authors.initials: MF
authors.lastname: Brivio
authors.forename: A
authors.initials: A
authors.lastname: Boselli
authors.forename: E
authors.initials: E
authors.lastname: Lavezzari
authors.forename: S
authors.initials: S
authors.lastname: Veraldi
authors.forename: M
authors.initials: M
authors.lastname: Bignotto
authors.forename: P
authors.initials: P
authors.lastname: Castano
mesh_terms: D017209:Apoptosis; D002454:Cell Differentiation; D002455:Cell Division; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008297:Male; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*
chemical_list: D016271:Proto-Oncogene Proteins c-myc