title: Sample preparation for high throughput accurate mass analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
abstract: An automated sample preparation for high throughput accurate mass determinations by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been developed. Sample preparation was performed with an automated workstation and automated mass analyses were performed with a commercial MALDI-TOF mass spectrometer. The method was tested with a 41-sample library. MALDI-TOFMS was found to give the needed sensitivity, accurate mass measurement, and soft ionization necessary for structure confirmation, even of mixtures. A mass accuracy of 5 ppm or less was obtained in over 80% of known compound measurements. A mass accuracy better than 10 ppm was obtained for all measurements of known compounds. Analyses of parallel synthesis products resulted in 77% of the measurements with a mass accuracy of 5 ppm or better.
authors.affiliation: Department of Chemistry & Biochemistry, University of Delaware, Newark, DE 19711, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Lake
authors.forename: M V
authors.initials: MV
authors.lastname: Johnson
authors.forename: C N
authors.initials: CN
authors.lastname: McEwen
authors.forename: B S
authors.initials: BS
authors.lastname: Larsen
mesh_terms: D001331:Automation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors