title: Interaction of myosin with F-actin: time-dependent changes at the interface are not slow.
abstract: The kinetics of formation of the actin-myosin complex have been reinvestigated on the minute and second time scales in sedimentation and chemical cross-linking experiments. With the sedimentation method, we found that the binding of the skeletal muscle myosin motor domain (S1) to actin filament always saturates at one S1 bound to one actin monomer (or two S1 per actin dimer), whether S1 was added slowly (17 min between additions) or rapidly (10 s between additions) to an excess of F-actin. The carbodiimide (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, EDC)-induced cross-linking of the actin-S1 complex was performed on the subsecond time scale by a new approach that combines a two-step cross-linking protocol with the rapid flow-quench technique. The results showed that the time courses of S1 cross-linking to either of the two actin monomers are identical: they are not dependent on the actin/S1 ratio in the 0.3-20-s time range. The overall data rule out a mechanism by which myosin rolls from one to the other actin monomer on the second or minute time scales. Rather, they suggest that more subtle changes occur at the actomyosin interface during the ATP cycle.
authors.affiliation: CRBM du Centre National de la Recherche Scientifique, IFR 24, Montpellier, France.
authors.forename: J
authors.initials: J
authors.lastname: Van Dijk
authors.forename: F
authors.initials: F
authors.lastname: Céline
authors.forename: T
authors.initials: T
authors.lastname: Barman
authors.forename: P
authors.initials: P
authors.lastname: Chaussepied
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D003432:Cross-Linking Reagents / Q000494:pharmacology; D019281:Dimerization; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D007700:Kinetics; D018482:Muscle, Skeletal / Q000378:metabolism; D015879:Myosin Subfragments / Q000737:chemistry / Q000378:metabolism; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D013997:Time Factors
chemical_list: D000199:Actins; D003432:Cross-Linking Reagents; D015879:Myosin Subfragments; D009218:Myosins; D005022:Ethyldimethylaminopropyl Carbodiimide