title: HPA-1, 3, 5 genotyping to establish a typed platelet donor panel.
abstract: Platelet antigen typing of blood donors is not routinely performed. Usually, ABO-matched platelets are transfused. In situations where the recipient has a human platelet-associated (HPA) antibody resulting in refractoriness to platelet transfusions, post-transfusion purpura or neonatal alloimmune thrombocytopenia, HPA-typed platelets may be required. Having a panel of HPA-typed donors would be helpful. This study utilised PCR-RFLP and PCR-SSP methods to determine the HPA-1, 3, 5 genotypes of 1,000 blood donors to establish a panel of HPA homozygous donors who could donate platelets for patients with anti-HPA-antibodies. The study demonstrates the HPA genotype frequencies in a Western Australian blood donor population and has identified donors who are homozygous for the "aa" and "bb" genotypes for HPA-1, 3 and 5. The Australian Red Cross Blood Service-NW Region can now use these data to develop a panel of HPA-1, 3 and 5 "aa" and "bb" homozygous platelet donors, which can be accessed for patients with HPA antibodies requiring platelet transfusion.
authors.affiliation: Department of Pathology, University of Western Australia.
authors.forename: J
authors.initials: J
authors.lastname: Verran
authors.forename: D
authors.initials: D
authors.lastname: Grey
authors.forename: J
authors.initials: J
authors.lastname: Bennett
authors.forename: J A
authors.initials: JA
authors.lastname: Lown
authors.forename: W N
authors.initials: WN
authors.lastname: Erber
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics* / Q000276:immunology; D001782:Blood Donors; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001792:Blood Platelets / Q000276:immunology*; D001803:Blood Transfusion; D004247:DNA / Q000032:analysis; D000939:Epitopes / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012042:Registries; D014904:Western Australia
chemical_list: D016824:Antigens, Human Platelet; D000939:Epitopes; D004247:DNA