title: Novel cytotoxic diterpenes from Casearia arborea.
abstract: Cytotoxicity-guided fractionation of the dichloromethane-methanol extract of the roots of Casearia arborea yielded five novel clerodane diterpenes, casearborins A-E (1-5), as well as cucurbitacin B. The presence of cucurbitacins glycosides was also detected. The absolute configuration of casearborin E was determined by X-ray crystallography.
authors.affiliation: Laboratory of Drug Discovery Research & Development, Developmental Therapeutics Program, Division of Cancer Treatment & Diagnosis, National Cancer Institute-FCRDC, Bldg. 1052, Rm. 121, Frederick, Maryland 21702-1201, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Beutler
authors.forename: K L
authors.initials: KL
authors.lastname: McCall
authors.forename: K
authors.initials: K
authors.lastname: Herbert
authors.forename: D L
authors.initials: DL
authors.lastname: Herald
authors.forename: G R
authors.initials: GR
authors.lastname: Pettit
authors.forename: T
authors.initials: T
authors.lastname: Johnson
authors.forename: R H
authors.initials: RH
authors.lastname: Shoemaker
authors.forename: M R
authors.initials: MR
authors.lastname: Boyd
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010946:Plants, Medicinal / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes