Major and 5S ribosomal sequences of the largemouth bass Micropterus salmoides (Perciformes, Centrarchidae) are localized in GC-rich regions of the genome.
Major and 5S ribosomal genes have been localized in the chromosomes of Micropterus salmoides. By C-banding, Ag-staining, CMA3-staining and 45S and 5S fluorescence in-situ hybridization (FISH), we demonstrate that the 45S and 5S ribosomal genes are clustered in two different chromosome pairs and both are located in heterochromatic GC-rich regions. PCR amplification and sequencing of the 5S intergenic non-transcribed sequences have allowed us to identify variability essentially due to a trinucleotide tandem repeat (GCT).
Deiana
A M
AM
Dipartimento di Biologia Animale ed Ecologia, Università di Cagliari, Italy. adeiana@unica.it
Cau
A
A
Salvadori
S
S
Coluccia
E
E
Cannas
R
R
Milia
A
A
Tagliavini
J
J
D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics*; D002871:Chromosome Banding; D004275:DNA, Ribosomal / Q000235:genetics*; D005260:Female; D005456:Fluorescent Dyes; D020862:GC Rich Sequence; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D016415:Sequence Alignment
D004275:DNA, Ribosomal; D005456:Fluorescent Dyes; D012341:RNA, Ribosomal, 5S