title: Determination of non-transferrin-bound iron in genetic hemochromatosis using a new HPLC-based method.
abstract: OBJECTIVE
  Non-transferrin-bound iron may play a major pathogenic role in iron overload diseases due to its high hepatic uptake and potential damaging effect. The aim of this study was to evaluate the relevance of measuring serum non-transferrin-bound iron levels in genetic hemochromatosis using a new high performance liquid chromatography-based method.
  
  
  METHODS
  This method includes a presaturation step of transferrin with cobalt(II) in order to avoid secondary deplacement of non-transferrin-bound iron toward transferrin during the assay. Six genetic hemochromatotic patients were followed serially during venesection treatment.
  
  
  CONCLUSIONS
  The results indicate: (i) that this new method permits detection of non-transferrin-bound iron when transferrin is not fully saturated, (ii) that non-transferrin-bound iron levels persist almost until the completion of treatment, (iii) that non-transferrin-bound iron levels are well correlated with transferrin saturation for a given patient, and (iv) that despite some individual variations, a transferrin saturation value lower than 35% usually corresponds to the disappearance of non-transferrin-bound iron.
authors.affiliation: INSERM U522, CHR Pontchaillou, Rennes, France.
authors.forename: O
authors.initials: O
authors.lastname: Loréal
authors.forename: I
authors.initials: I
authors.lastname: Gosriwatana
authors.forename: D
authors.initials: D
authors.lastname: Guyader
authors.forename: J
authors.initials: J
authors.lastname: Porter
authors.forename: P
authors.initials: P
authors.lastname: Brissot
authors.forename: R C
authors.initials: RC
authors.lastname: Hider
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006432:Hemochromatosis / Q000097:blood* / Q000235:genetics; D006801:Humans; D007501:Iron / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D012015:Reference Standards; D014168:Transferrin / Q000378:metabolism*
chemical_list: D014168:Transferrin; D007501:Iron