title: Proliferative activity of gastric epithelial cells in Helicobacter pylori infected children.
abstract: Helicobacter pylori infection has been associated with gastric carcinogenesis. Gastric epithelial cells proliferative rate is accelerated in H. pylori infected adult patients. Our study was performed to evaluate proliferative cell activity in gastric epithelium in the course of H. pylori infection in the early stage of its natural history. Gastric antral biopsy specimens were obtained from thirteen H. pylori positive and seven negative children. To assess replication rates we used nucleolar organiser regions staining with colloidal silver nitrate technique (AgNOR). The number of AgNORs per nucleus, area of single AgNOR, and the quotient of these two parameters (AgNOR content) were analysed. The mean area of AgNOR was lower in H. pylori positive than in negative children. Conversely, both the mean number of AgNOR per nucleus and AgNOR content were higher in infected than non infected subjects. These results show accelerated proliferation of gastric antral epithelial cells in the course of H. pylori infection in children. Such alteration of cell replication occurring in an initial phase of natural history of long lasting infection provides an explanation for the association between acquisition of H. pylori infection in the first years of life and the development of gastric cancer.
authors.affiliation: Department of Human Anatomy and Histology, Military Medical Academy, Lódź, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Janas
authors.forename: S
authors.initials: S
authors.lastname: Orkisz
authors.forename: H
authors.initials: H
authors.lastname: Bartel
authors.forename: E
authors.initials: E
authors.lastname: Czkwianianc
authors.forename: I
authors.initials: I
authors.lastname: Płaneta-Małecka
authors.forename: S
authors.initials: S
authors.lastname: Suski
mesh_terms: D000293:Adolescent; D002455:Cell Division / Q000502:physiology; D002648:Child; D004847:Epithelial Cells / Q000166:cytology* / Q000382:microbiology; D005753:Gastric Mucosa / Q000166:cytology / Q000382:microbiology; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori; D006801:Humans; D011706:Pyloric Antrum / Q000166:cytology* / Q000382:microbiology; D016622:Silver Staining