title: Long-term evolution of local, proximal and remote astrocyte responses after diverse nucleus basalis lesioning (an experimental Alzheimer model): GFAP immunocytochemical study.
abstract: A study on long-term astrocytic responses (from 1 day to 20 months after lesioning in 4-month-old rats, and from 1 day to 6 months in 20-month-old rats) to diverse unilateral damage of the nucleus basalis (nbM) by injection of 40 nmol of ibotenic acid, or 50 or 100 nmols of quisqualic acid was performed using a histochemical method (immunoreactivity against the glial fibrillary acidic protein GFAP). Glial reactivity (i.e., isolated or clustered hypertrophic and/or hyper-reactive astrocytes) was evaluated in several ipsilateral and contralateral brain regions: the 'local response' within the damaged nbM region; the 'proximal response' (a new concept proposed by us) in the non-damaged structures neighbouring the nbM; and the 'remote response' in the ipsilateral brain cortex and in the contralateral cortex and nbM. In 4-month-old animals, the remote cortical glial responses, independent of the involution of cortical cholinergic activity and randomly located in layers I-V of motor and somatosensory cortical regions, were similar in appearance over a long period (13-20 months), with the highest reactivity 45 days after lesioning. The proximal response lasted from 1 day to 13 months and afterwards tended to disappear. Contralateral reactivity and ipsilateral cortical scars were observed. The local (nbM) glial response was maintained throughout the period studied. Subsets of astrocytes of different reactivities were observed, most of their elements being highly intermeshed. In 20-month-old animals, nbM lesions produced less positive, but similar, glial reactive patterns. This glial reactivity was superposed onto the glial reactivity of old age. All these results are discussed. The maintenance of reactive astrocytes many months after lesioning suggests the existence of cellular factors other than those produced by damaged nbM neurons. Taking into account the role of glial cells under pathological conditions, it is possible that these reactive astrocytes in humans could promote neurodegenerative processes, such as amyloid plaque formation and neurodegeneration (Alzheimer's disease). Along this line, nbM cholinergic involution could then originate cortical involution through induced reactive astrocytosis.
authors.affiliation: Department of Morphology (Cellular Biology Section), Faculty of Health Sciences, University of Las Palmas, Gran Canaria, Canary Islands, Las Palmas, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Monzón-Mayor
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: J
authors.initials: J
authors.lastname: Arbelo-Galván
authors.forename: M
authors.initials: M
authors.lastname: Romero-Alemán
authors.forename: C
authors.initials: C
authors.lastname: Yanes
authors.forename: M L
authors.initials: ML
authors.lastname: Plaza
authors.forename: J R
authors.initials: JR
authors.lastname: Rodríguez
authors.forename: J J
authors.initials: JJ
authors.lastname: Rodríguez
authors.forename: A
authors.initials: A
authors.lastname: Toledano
mesh_terms: D000367:Age Factors; D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D020532:Basal Nucleus of Meynert / Q000293:injuries / Q000378:metabolism*; D004195:Disease Models, Animal; D005625:Frontal Lobe / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010296:Parietal Lobe / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein