Timecourse study of UVB-induced cytokine induction in whole mouse skin.
Ever since the skin was recognized as a site of immunologic activity, a number of laboratories have studied the production of cytokines by skin cells and the effects of chemicals, allergens, contact irritants and UVB radiation on their production. Most research to date has been carried out using either purified populations of primary cells, or established cell lines. Cytokines, however, do not function in isolation but they appear in human tissues within the context of other cytokines that can, in turn, strongly influence the final biological outcome. Therefore, in vivo studies using whole skin are more physiologically relevant since all cell types are present and interactions among them are allowed to proceed. We report here the results of a detailed timecourse study using whole mouse skin, consisting of both dermis and epidermis, irradiated with either a low or high dose of UVB and analyzed using a Multi-probe RNase protection assay system. The results show that in whole skin the kinetics of cytokine induction are different than what was previously observed in tissue culture. In addition to already known skin-associated cytokines, we report here the presence and UVB induction of cytokines not previously reported.
Scordi
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Department of Microbiology and Immunology, University of Miami School of Medicine, Florida, USA.
Vincek
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D000818:Animals; D001345:Autoradiography; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000528:radiation effects*; D020405:Dermis / Q000276:immunology / Q000528:radiation effects; D004817:Epidermis / Q000276:immunology / Q000528:radiation effects; D005260:Female; D006131:Growth Inhibitors / Q000528:radiation effects; D006801:Humans; D007375:Interleukin-1 / Q000528:radiation effects; D016753:Interleukin-10 / Q000528:radiation effects; D017370:Interleukin-11 / Q000528:radiation effects; D007376:Interleukin-2 / Q000528:radiation effects; D015850:Interleukin-6 / Q000528:radiation effects; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000528:radiation effects; D008233:Lymphotoxin-alpha / Q000528:radiation effects; D016173:Macrophage Colony-Stimulating Factor / Q000528:radiation effects; D008263:Macrophage Migration-Inhibitory Factors / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D015347:RNA Probes; D011829:Radiation Dosage; D012260:Ribonucleases; D012867:Skin / Q000276:immunology / Q000528:radiation effects*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000145:classification*
D016207:Cytokines; D006131:Growth Inhibitors; D007375:Interleukin-1; D017370:Interleukin-11; D007376:Interleukin-2; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D008233:Lymphotoxin-alpha; D008263:Macrophage Migration-Inhibitory Factors; D015347:RNA Probes; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D016173:Macrophage Colony-Stimulating Factor; D012260:Ribonucleases