title: Fasciola hepatica: an antigen fraction derived from newly excysted juveniles, containing an immunoreactive 32-kDa protein, induces strong protective immunity in rats.
abstract: Crude antigens of adult Fasciola hepatica and of newly excysted juveniles (NEJ) and a low-molecular-weight fraction of antigen from NEJs were tested for inducing protective immunity in rats. Two routes of vaccination were applied. The results showed that intraperitoneal vaccination induced significantly better protection (P <0.05) than intramuscular vaccination. Intraperitoneal vaccination with antigens from NEJs induced more effective protection: after challenge infection, rats that were so vaccinated had 92.6% (+/-2.5% SEM) fewer parasites in their liver and 57.3% (+/-13.3% SEM) fewer parasites penetrating the gut wall than control rats. Rats that were vaccinated with a low-molecular-weight fraction of antigen from NEJs were also highly protected against a challenge. F. hepatica antigens that are immunoreactive were identified on immunoblots, using sera collected from highly protected rats that had been vaccinated with NEJ antigens and also sera from cattle and rats that were experimentally infected with F. hepatica. The low-molecular-weight fraction of antigen from NEJs contained an immunodominant 32-kDa protein that was recognized by serum antibodies of vaccinated rats and immune cattle. This 32-kDa protein was not detected in partially purified antigens from adult flukes. We conclude that antigens of NEJs of F. hepatica, when injected intraperitoneally in rats, are highly protective. In particular, the 32-kDa protein contained in these antigens may be highly valuable for the development of an effective vaccine against F. hepatica.
authors.affiliation: Department of Immunology, DLO-Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
authors.forename: F J
authors.initials: FJ
authors.lastname: van Milligen
authors.forename: J B
authors.initials: JB
authors.lastname: Cornelissen
authors.forename: B A
authors.initials: BA
authors.lastname: Bokhout
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000517:prevention & control*; D005260:Female; D007117:Immunization, Secondary; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000379:methods*; D014612:Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D014612:Vaccines