Quantitative analysis of enantioselective enzymatic hydrolysis with non-enantioselective removal of chiral products.
Kinetic resolution of racemic compounds by enzymatic hydrolysis with non-enantioselective separation of enantiomer products via a separator or ion-pair formation has been quantitatively analyzed. Theoretical results indicate that the removal of chiral products has profound effects on improving the conversion and enantiomeric excess for the desired chiral substrate or product. The analysis was confirmed from lipase-catalyzed hydrolysis of racemic methyl 2-chloropropionate in the presence of pyrrolidine in buffer saturated dichloromethane.
Cheng
IC
Department of Chemical Engineering, National Cheng Kung, University, 70101, Tainan, Taiwan
Tsai
SW