Response to nitrogen starvation in Corynebacterium glutamicum.
Proteins strongly synthesized in Corynebacterium glutamicum during nitrogen restriction were examined by two-dimensional gel electrophoresis and microsequencing. Two main groups of enzymes were identified beside miscellaneous proteins, enzymes involved (i) in protein synthesis, and (ii) in carbon metabolism. Biochemical measurements revealed an increase of oxygen consumption during nitrogen starvation, indicating an enhanced energy demand of the cells. By Northern hybridizations, an increased transcription for the gap and fda genes upon nitrogen deprivation was shown.
Schmi
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R
ABteilung Mikrobiologie, Universität Osnabrück, Germany.
Uhlemann
E M
EM
Nolden
L
L
Wersch
G
G
Hecker
R
R
Hermann
T
T
Marx
A
A
Burkovski
A
A
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D015152:Blotting, Northern; D003352:Corynebacterium / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D009584:Nitrogen; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D001426:Bacterial Proteins; D003470:Culture Media; D012329:RNA, Bacterial; D012333:RNA, Messenger; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005634:Fructose-Bisphosphate Aldolase; D009584:Nitrogen