title: Anti-sickling, analgesic and anti-inflammatory properties of 3,5-dimethoxy-4-hydroxy benzoic acid and 2,3,4-trihydroxyacetophenone.
abstract: Effects of 3,5-dimethoxy-4-hydroxybenzoic acid and 2,3,4-trihydroxyacetophenone were studied on haemoglobin S (Hb S) polymerisation, analgesia and inflammation using Hb S solution, rats and mice. UV spectrophotometric procedure was used to monitor the polymerization of the Hb S. Acetic acid induced writhing in mice and egg albumin induced rat paw edema procedures were used to evaluate analgesic and anti-inflammatory activities of the compounds respectively. The results indicate that both drugs inhibit the process of polymerization significantly, possibly by direct action on the Hb S molecules. The drugs inhibited acetic acid induced pain and decreased egg albumin induced oedema. It is concluded that 3,5-dimethoxy-4-hydroxybenzoic acid and 2,3,4-trihydroxyacetophenone may have some value in the management of sickle cell disease.
authors.affiliation: Department of Pharmacology & Toxicology, NIPRD, Abuja, Nigeria.
authors.forename: K
authors.initials: K
authors.lastname: Gamaniel
authors.forename: B B
authors.initials: BB
authors.lastname: Samuel
authors.forename: D S
authors.initials: DS
authors.lastname: Kapu
authors.forename: A
authors.initials: A
authors.lastname: Samson
authors.forename: H
authors.initials: H
authors.lastname: Wagner
authors.forename: J I
authors.initials: JI
authors.lastname: Okogun
authors.forename: C
authors.initials: C
authors.lastname: Wambebe
mesh_terms: D019342:Acetic Acid; D000098:Acetophenones / Q000494:pharmacology* / Q000633:toxicity; D000700:Analgesics / Q000494:pharmacology*; D000755:Anemia, Sickle Cell / Q000097:blood; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D000986:Antisickling Agents / Q000494:pharmacology*; D004487:Edema; D005260:Female; D006168:Guinea Pigs; D006451:Hemoglobin, Sickle / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology* / Q000633:toxicity; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010047:Ovalbumin; D010146:Pain; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: C102214:2',3,4-trihydroxyacetophenone; C413324:3,5-dimethoxy-4-hydroxybenzoic acid; D000098:Acetophenones; D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000986:Antisickling Agents; D006451:Hemoglobin, Sickle; D062385:Hydroxybenzoates; D010047:Ovalbumin; D019342:Acetic Acid