Electron spin resonance studies of dipalmitoylphosphatidylcholine liposomes containing soybean-derived sterylglucoside.
The effects of soybean-derived sterylglucoside (SG) on the fluidity of liposomal membrane composed of dipalmitoylphosphatidylcholine (DPPC) were investigated compared with those of soybean-derived sterol (SS) and cholesterol (Ch) using an electron spin resonance spectrometer. Three kinds of liposomes were prepared in the molar ratio of DPPC/X=7/4, where X is SS, Ch or SG. The fluidity close to the polar head groups increased with an increase of temperature in the DPPC membrane containing SS, Ch and SG in the range 35 to 45 degrees C. Those near the hydrophobic end changed with an increase in temperature in liposomes containing SS, Ch and SG, which had a fluidizing effect on the DPPC membrane below the transition temperature (Tm, 41.9 degrees C) and a condensing effect over the Tm. The fluidizing effects of these compounds around 37 degrees C near the polar head group and the hydrophobic end increased in the following order: Ch < SG < or = SS and SS < Ch < SG, respectively. SG increased the fluidity of liposomal membrane dramatically above the Tm (35.4 degrees C). These results suggest that the high fluidity close to the hydrophobic end of the liposomal membranes around 37 degrees C, the decrease of Tm, and the sigmoidal nature of fluidity vs. temperature are important factors in the effectiveness of liposomes containing SG as a carrier of drugs.
Muramatsu
K
K
Department of Pharmaceutics, Hoshi University, Tokyo, Japan.
Masumizu
T
T
Maitani
Y
Y
Hwang
S H
SH
Kohno
M
M
Takayama
K
K
Nagai
T
T
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002782:Cholestenes / Q000737:chemistry*; D004337:Drug Carriers; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers; D008081:Liposomes / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
D002782:Cholestenes; D004337:Drug Carriers; D008051:Lipid Bilayers; D008081:Liposomes; C096056:soy derived sterylglucoside mixture; D015060:1,2-Dipalmitoylphosphatidylcholine