title: Dissociation of mitochondrial malate dehydrogenase into active soluble subunits.
abstract: Gel exclusion chromatographic studies demonstrate that cytosolic and mitochondrial malate dehydrogenases (cMDH and mMDH) dissociate into subunits in the presence of 0.1% of the non-ionic detergent Triton X-100 (TX-100). The presence of cofactor and catalytically competent cofactor-substrate pairs does not protect mMDH against this dissociation. In contrast, cMDH dimers resist dissociation in the presence of either addition. Since steady state kinetic studies indicate both enzymes are fully active in the presence of 0.1% TX-100, we conclude that quaternary structure is not a kinetically important feature of mMDH structure and cooperativity does not account for mMDH kinetic anomalies. In contrast, cooperativity is a reasonable explanation for cMDH kinetic properties even in the presence of 0.1% TX-100, since this enzyme's subunits associate in the presence of active site ligands. The existence of fully active mMDH subunits raises the possibility that this species rather than the dimer may be a constituent of proposed multi-enzyme complexes of the mitochondrion. Preliminary chromatographic experiments involving gently disrupted mitochondria have found MDH activity in differently sized complexes, all with molecular weights larger than the mMDH dimer but smaller than complexes anticipated for multi-enzyme complexes involving other enzymes and the mMDH dimer.
authors.affiliation: Electronic Data Systems, Dayton, OH 45439, USA.
authors.forename: S
authors.initials: S
authors.lastname: Shaw
authors.forename: R
authors.initials: R
authors.lastname: Geyer
authors.forename: G M
authors.initials: GM
authors.lastname: Alter
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000737:chemistry*; D008928:Mitochondria / Q000201:enzymology*; D008929:Mitochondria, Heart / Q000201:enzymology; D008970:Molecular Weight; D017830:Octoxynol; D013552:Swine
chemical_list: D017830:Octoxynol; D008291:Malate Dehydrogenase