Construction and evaluation of a capillary array DNA sequencer based on a micromachined sheath-flow cuvette.
A capillary array electrophoresis DNA sequencer is reported based on a micromachined sheath-flow cuvette as the detection chamber. This cuvette is equipped with a set of micromachined features that hold the capillaries in precise registration to ensure uniform spacing between the capillaries, in order to generate uniform hydrodynamic flow in the cuvette. A laser beam excites all of the samples simultaneously, and a microscope objective images fluorescence onto a set of avalanche photodiodes, which operate in the analog mode. A high-gain transimpedance amplifier is used for each photodiode, providing high duty-cycle detection of fluorescence.
Crabtree
H J
HJ
Department of Chemistry, University of Alberta, Edmonton, Canada.
Bay
S J
SJ
Lewis
D F
DF
Zhang
J
J
Coulson
L D
LD
Fitzpatrick
G A
GA
Delinger
S L
SL
Harrison
D J
DJ
Dovichi
N J
NJ
D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods; D005069:Evaluation Studies as Topic; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods
D004279:DNA, Viral; D004247:DNA