title: A dual role of 20-hydroxyecdysone in the control of protein synthesis related to DNA puff activity in the anterior region of Bradysia hygida (Diptera, Sciaridae) salivary gland.
abstract: During the last 30 h of the larval stage, the salivary glands of Bradysia hygida show the amplification of some genes, resulting in the formation of two successive groups of DNA puffs, which direct the synthesis of two different sets of polypeptides. Incubation of anterior (S1) salivary gland regions, at age E7, beginning of first group of DNA puffs activity, in culture medium for 2 to 10 h results in a decrease in the synthesis of the polypeptides characteristic of this period. However, during subsequent incubation (from E7 to E7+12 h-24 h), when the second group of DNA puffs is active, S1 regions were able to synthesize some polypeptides characteristic of this period. The role of 20-OH ecdysone was studied, in vitro and in vivo, during these two periods of protein synthesis in S1 regions. The presence of the hormone was shown to be necessary to maintain, in vitro, the synthesis of the first set of polypeptides and was strongly inhibitory, in vitro and in vivo, to the synthesis of the second set of polypeptides. Thus, it is likely that the activity of the two distinct groups of DNA puffs is under opposite 20-OH-ecdysone control mechanisms.
authors.affiliation: Departamento de Morfologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900, SP, Ribeirão Preto, Brazil.
authors.forename: D P
authors.initials: DP
authors.lastname: de Carvalho
authors.forename: P S
authors.initials: PS
authors.lastname: Coelho
authors.forename: J C
authors.initials: JC
authors.lastname: de Almeida
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004175:Diptera / Q000235:genetics*; D004441:Ecdysterone / Q000494:pharmacology*; D005784:Gene Amplification; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000254:growth & development; D014176:Protein Biosynthesis; D012469:Salivary Glands / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D004441:Ecdysterone; D004247:DNA