Rp-cAMPS has no effect on adenosine A1 receptors in guinea-pig cardiomyocytes.
Rp-cAMPS, a protein kinase A inhibitor, is used in the investigation of the cAMP-dependent systems. A report by Musgrave et al. has suggested that Rp-cAMPS may also act on adenosine receptors. To determine whether this occurs in guinea-pig ventricular myocytes, Rp-cAMPS was applied in the presence and absence of DCPCX, an adenosine A receptor antagonist. The isoprenaline-induced response was significantly decreased by Rp-cAMPS and the effect was not altered by the presence of DCPCX. Therefore Rp-cAMPS has no effect on cell contraction via adenosine A1 receptors and can reliably be used to investigate cyclic AMP-dependent systems in isolated cardiac myocytes.
Adamson
D L
DL
Imperial College of Science Technology and Medicine, National Heart and Lung Institute, London, UK. d.adamson@ic.ac.uk
Harding
S E
SE
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006168:Guinea Pigs; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D018047:Receptors, Purinergic P1 / Q000187:drug effects*; D013873:Thionucleotides / Q000494:pharmacology*; D014970:Xanthines / Q000494:pharmacology
D004791:Enzyme Inhibitors; D018047:Receptors, Purinergic P1; D013873:Thionucleotides; D014970:Xanthines; C016957:adenosine-3',5'-cyclic phosphorothioate; C051360:1,3-dipropyl-8-cyclopentylxanthine; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D000241:Adenosine; D007545:Isoproterenol