title: Continuing damage to rat retinal DNA during darkness following light exposure.
abstract: The damaging effects of visible light on the mammalian retina can be detected as functional, morphological or biochemical changes in the photoreceptor cells. Although previous studies have implicated short-lived reactive oxygen species in these processes, the termination of light exposure does not prevent continuing damage. To investigate the degenerative processes persisting during darkness following light treatment, rats were exposed to 24 h of intense visible light and the accumulation of DNA damage to restriction fragments containing opsin, insulin 1 or interleukin-6 genes was measured as single-strand breaks (ssb) on alkaline agarose gels. With longer dark treatments all three DNA fragments showed increasing DNA damage. Treatment of rats with the synthetic antioxidant dimethylthiourea prior to light exposure reduced the initial development of alkali-sensitive strand breaks and allowed significant repair of all three DNA fragments. The time course of double-strand DNA breaks was also examined in specific genes and repetitive DNA. Nucleosomal DNA laddering was evident immediately following the 24 h light treatment and increased during the subsequent dark period. The increase in the intensity of the DNA ladder pattern suggests a continuation of enzymatically mediated apoptotic processes triggered during light exposure. The protective effects of antioxidant suggests that the light-induced DNA degradative process includes both early oxidative reactions and enzymatic processes that continue after cessation of light exposure.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wright State University School of Medicine, Dayton, OH 45435, USA.
authors.forename: S
authors.initials: S
authors.lastname: Specht
authors.forename: D T
authors.initials: DT
authors.lastname: Organisciak
authors.forename: R M
authors.initials: RM
authors.lastname: Darrow
authors.forename: M
authors.initials: M
authors.lastname: Leffak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D003624:Darkness; D008027:Light; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000975:Antioxidants; C038983:1,3-dimethylthiourea; D004247:DNA; D013890:Thiourea