Altered behaviour following RNA interference knockdown of a C. elegans G-protein coupled receptor by ingested double stranded RNA.
Using a systemic and continuous delivery method based on feeding on a particular strain of transformed Escherichia coli to induce double stranded RNA-mediated interference, we targeted the product of the npr-1 gene, a putative Caenorhabditis elegans homologue of a neuropeptide Y receptor, a G-protein coupled receptor. We were able to reproduce the social behaviour observed for the naturally occurring npr-1 mutant when wild type N2 Bristol eggs developed in a lawn of bacteria producing double stranded RNA for npr-1. This facile approach may also be useful when studying the function of other worm G-protein coupled receptors.
Vaz Gomes
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A
Center for Genomics Research, Karolinska Institutet, Berzelius väg 37, S-171 77, Stockholm, Sweden. ana.vaz.gomes@ccgr.ki.se
Wahlestedt
C
C
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods; D009154:Mutation; D012330:RNA, Double-Stranded / Q000008:administration & dosage* / Q000235:genetics; D017476:Receptors, Neuropeptide Y / Q000235:genetics*
D012330:RNA, Double-Stranded; D017476:Receptors, Neuropeptide Y