title: Suppression of a DnaX temperature-sensitive polymerization defect by mutation in the initiation gene, dnaA, requires functional oriC.
abstract: Temperature sensitivity of DNA polymerization and growth, resulting from mutation of the tau and gamma subunits of Escherichia coli DNA polymerase III, are suppressed by Cs,Sx mutations of the initiator gene, dnaA. These mutations simultaneously cause defective initiation at 20 degrees C. Efficient suppression, defined as restoration of normal growth rate at 39 degrees C to essentially all the cells, depends on functional oriC. Increasing DnaA activity in a strain capable of suppression, by introducing a copy of the wild-type allele, increasing the suppressor gene dosage or introducing a seqA mutation, reversed the suppression. This suggests that the suppression mechanism depends on reduced activity of DnaACs, Sx. Models that assume that suppression results from an initiation defect or from DnaACs,Sx interaction with polymerization proteins during nascent strand synthesis are proposed.
authors.affiliation: Section of Molecular Genetics and Microbiology, and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
authors.forename: A
authors.initials: A
authors.lastname: Blinkova
authors.forename: E
authors.initials: E
authors.lastname: Ginés-Candelaria
authors.forename: J D
authors.initials: JD
authors.lastname: Ross
authors.forename: J R
authors.initials: JR
authors.lastname: Walker
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D004258:DNA Polymerase III / Q000235:genetics*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D016296:Mutagenesis; D011108:Polymers; D018741:Replication Origin; D013489:Suppression, Genetic; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; C103076:DnaX protein, Bacteria; D011108:Polymers; D004258:DNA Polymerase III