Analysis of BRCA1 involvement in breast cancer in Indian women.
The involvement of the familial breast-ovarian cancer gene (BRCA1) in the molecular pathogenesis of breast cancer among Indian women is unknown. We have used a set of microsatellite polymorphisms to examine the frequency of allele loss at the BRCA1 region on chromosome 17q21, in a panel of 80 human breast tumours. Tumour and blood leukocyte/normal tissue DNA from a series of 80 patients with primary breast cancer was screened by PCR-amplified microsatellite length polymorphisms to detect deletions at three polymorphic BRCA1 loci. PCR-allelotype was valuable in examining allele losses from archival and small tumour samples. Loss of alleles at BRCA1 in the patient set, confirmed a noteworthy role of this gene in the molecular patho-genesis of breast cancer and was in accordance with its well-documented tumour suppressive function.
Pestonjamasp
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PH
Division of Laboratory Medicine, Tata Memorial Hospital, Ernest Borges Road, Parel, Mumbai 400 012, India.
Mittra
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D000483:Alleles; D019313:BRCA1 Protein / Q000032:analysis*; D001943:Breast Neoplasms / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D005260:Female; D006801:Humans; D007194:India; D019656:Loss of Heterozygosity; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics*; D016133:Polymerase Chain Reaction
D019313:BRCA1 Protein