title: Trophic interactions between brain-derived neurotrophic factor and s100beta on cultured serotonergic neurons.
abstract: Brain-derived neurotrophic factor (BDNF) and S100beta stimulate serotonergic neurons in fetal rat raphe primary cultures grown under serum-free conditions. BDNF (50 ng/ml) treatment for 3 h enhanced S100beta immunoreactivity in both raphe and hippocampal glial cells. Combined treatment with BDNF and S100beta for 3 days increased the soma area of 5-HT neurons, but not the neurite length. Our results suggest that BDNF and S100beta, which regulate different signal transduction cascades, interact to exert complimentary effects on neuronal maturation by acting sequentially, not concurrently.
authors.affiliation: Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan. nmayumi@koto.kpu-m.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Nishi
authors.forename: M
authors.initials: M
authors.lastname: Kawata
authors.forename: E C
authors.initials: EC
authors.lastname: Azmitia
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004347:Drug Interactions; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D011903:Raphe Nuclei / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D012701:Serotonin / Q000493:pharmacokinetics*; D014316:Tritium
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002135:Calcium-Binding Proteins; D016895:Culture Media, Serum-Free; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D014316:Tritium; D012701:Serotonin; D020928:Mitogen-Activated Protein Kinases