A putative hyperglycemic factor from the cerebral ganglia of Otala lactea (Mollusca: Pulmonata).
Mantle tissue pieces from adult Otala lactea continuously synthesized glycogen over a 72-h incubation period. Acid-saline extract of the cerebral ganglia inhibited glycogen synthesis by mantle tissue in vitro. This effect was dose-dependent. The glycogen reduction factor from the cerebral ganglia was heat stable, protease sensitive, and relatively hydrophobic. The cerebral ganglia extract also stimulated mantle glycogen phosphorylase in vitro in a dose-dependent manner. The results suggest the presence of a hyperglycemic factor in the cerebral ganglia of Otala. The molecular weight of this factor, estimated by size-exclusion chromatography, was approximately 10,000. Mammalian glucagon had no significant effect on glycogen synthesis by the mantle pieces.
Abdraba
A M
AM
Department of Biology, York University, Toronto, Ontario, Canada.
Saleuddin
A S
AS
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D017952:Ganglia, Invertebrate / Q000737:chemistry / Q000201:enzymology; D005934:Glucagon / Q000378:metabolism; D005947:Glucose / Q000493:pharmacokinetics; D006003:Glycogen / Q000096:biosynthesis; D006943:Hyperglycemia / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism; D012908:Snails / Q000378:metabolism*
D002457:Cell Extracts; D006003:Glycogen; D005934:Glucagon; D006005:Phosphorylases; D005947:Glucose