Crystallization and preliminary X-ray diffraction analysis of Saccharomyces cerevisiae Ygr203p, a homologue of Acr2 arsenate reductase.
Ygr203p, a 148-residue protein encoded by the ygr203w gene of Saccharomyces cerevisiae, is a homologue of the yeast Acr2 arsenate reductase encoded by the acr2 (or ypr200c) gene. It also shows significant sequence similarity to the human cell-cycle control Cdc25 phosphatase family. It has been overexpressed in soluble form in Escherichia coli with a His(6) tag at its C-terminus. The recombinant protein has been crystallized at 296 K using sodium chloride as precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 40.48, b = 50.95, c = 91.95 A. The asymmetric unit contains a monomer, giving a crystal volume per protein mass (V(m)) of 2.61 A(3) Da(-1) and a solvent content of 53.8%. The crystals diffract to better than 1.9 A resolution with Cu Kalpha X-rays. They are therefore suitable for high-resolution structure determination.
Moon
J
J
Department of Chemistry, College of Natural Sciences, Seoul National University, South Korea.
Kim
Y S
YS
Lee
J Y
JY
Cho
S J
SJ
Song
H K
HK
Cho
J H
JH
Kim
B M
BM
Kim
K K
KK
Suh
S W
SW
D000251:Adenosine Triphosphatases / Q000737:chemistry*; D053502:Arsenate Reductases; D053501:Arsenite Transporting ATPases; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D005822:Genetic Vectors / Q000138:chemical synthesis; D016623:Ion Pumps; D008970:Molecular Weight; D009097:Multienzyme Complexes; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D020687:cdc25 Phosphatases / Q000737:chemistry
D005656:Fungal Proteins; D016623:Ion Pumps; D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C506971:ARR2 protein, S cerevisiae; D053502:Arsenate Reductases; D020687:cdc25 Phosphatases; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases