Lumbar catheterization of the subarachnoid space with a 32-gauge polyurethane catheter in the rat.
Chronic catheterization of the subarachnoid space of rats is an important tool for intrathecal drug delivery in pharmacologic investigations of pain. We describe a technique using direct lumbar insertion of a small 32-gauge polyurethane (PU) catheter without extensive surgery. Location of the catheter was confirmed with 2% lidocaine injection 1 day later, and methylene blue injection after 7-14 days. This method improved recovery of the rat after catheter implantation and reduced neurologic complications.
Pogatzki
E M
EM
Department of Anesthesia, University of Iowa College of Medicine, Iowa, USA.
Zahn
P K
PK
Brennan
T J
TJ
D000818:Animals; D002404:Catheterization / Q000295:instrumentation / Q000379:methods*; D007278:Injections, Spinal / Q000295:instrumentation / Q000379:methods*; D008297:Male; D011140:Polyurethanes; D051381:Rats; D017207:Rats, Sprague-Dawley; D013346:Subarachnoid Space
D011140:Polyurethanes