Time-dependent closed form solutions for fully competitive enzyme reactions.
An analytic formalism developed earlier to describe the time evolution of the basic enzyme reaction is extended to fully competitive systems. Time-dependent closed form solutions are derived for the three nominal cases of competition: even, slow and fast inhibitors, allowing for the first time the complete characterization of the reactions. In agreement with previous work, the time-independent Michaelis-Menten approach is shown to be inaccurate when a fast inhibitor is present. The validity of the quasi-steady-state approximation on which the present framework is based is also revised.
Schnell
S
S
Centre for Mathematical Biology, Mathematical Institute, Oxford, U.K. schnell@maths.ox.ac.uk
Mendoza
C
C
D004791:Enzyme Inhibitors / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D013997:Time Factors
D004791:Enzyme Inhibitors; D004798:Enzymes