Fluorescent amplified-fragment length polymorphism analysis of the MRSA epidemic.
Genotypes and putative genetic relationships were characterised for epidemic methicillin-resistant Staphylococcus aureus (EMRSA) strains and isolates from England and Wales, using a high resolution DNA fingerprinting technique, fluorescent amplified-fragment length polymorphism (FAFLP). Each of the phage types of EMRSA had a distinct FAFLP profile. The technique revealed clusters of strains and isolates, and could distinguish isolates belonging to the same phage type. FAFLP provides a new approach to the epidemiological study and control of MRSA.
Grady
R
R
Molecular Biology Unit, Central Public Health Laboratory, London, UK.
O'Neill
G
G
Cookson
B
B
Stanley
J
J
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004739:England / Q000453:epidemiology; D006801:Humans; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance; D017720:Molecular Epidemiology; D010406:Penicillins / Q000494:pharmacology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D014852:Wales / Q000453:epidemiology
D004269:DNA, Bacterial; D010406:Penicillins; D008712:Methicillin