Controlled release of platelet-derived growth factor-BB from chondroitin sulfate-chitosan sponge for guided bone regeneration.
Platelet-derived growth factor-BB (PDGF-BB) releasing porous chondroitin-4-sulfate (CS)-chitosan sponge was designed with an aim of controlling growth factor delivery in order to improve bone formation. Porous CS-chitosan sponge was fabricated by freeze drying and crosslinking aqueous CS-chitosan solution. PDGF-BB was incorporated into the CS-chitosan sponge by soaking CS-chitosan sponge into the PDGF-BB solution. CS-chitosan sponge retained a porous structure with a 150-200-microm pore diameter that was suitable for cellular migration and osteoid ingrowth. Release rate of PDGF-BB could be controlled by varying the composition of CS in the sponge or initial loading content of PDGF-BB. CS-chitosan sponge induced increased osteoblast migration and proliferation as compared with chitosan sponge alone. Furthermore, the release of PDGF-BB from CS-chitosan sponge significantly enhanced osteoblast proliferation. These results suggest that PDGF-BB-releasing CS-chitosan sponge may be beneficial to enhance bone cell adaptation and regenerative potential when applied in wound sites.
Park
Y J
YJ
Department of Industrial Pharmacy, College of Pharmacy, Ewha Womans University, 11-1 Daehyun-Dong, Seodaemun-Ku, 120-750, Seoul, South Korea.
Lee
Y M
YM
Lee
J Y
JY
Seol
Y J
YJ
Chung
C P
CP
Lee
S J
SJ
D000818:Animals; D000077214:Becaplermin; D001861:Bone Regeneration / Q000187:drug effects*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D002809:Chondroitin Sulfates / Q000737:chemistry*; D003692:Delayed-Action Preparations; D005079:Excipients; D005347:Fibroblasts; D005881:Gingiva / Q000166:cytology; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage* / Q000494:pharmacology; D016062:Porosity; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012886:Skull / Q000166:cytology
D003692:Delayed-Action Preparations; D005079:Excipients; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D002686:Chitin; D000077214:Becaplermin; D002809:Chondroitin Sulfates; D048271:Chitosan