title: Monoclonal antibody 57B stains tumor tissues that express gene MAGE-A4.
abstract: Monoclonal antibody (Mab) 57B, which was raised against a recombinant MAGE-A3 protein, was tested for its ability to stain cells expressing various members of the MAGE-A gene family. COS-7 cells transfected with cDNAs encoding MAGE-A1, A2, A3, A4, A6, or A12 were stained, whereas those transfected with MAGE-A8, A9, A10, or A11 cDNAs were not. However, in tissue sections, we observed a different pattern of staining: the antibody effectively stained the tumors that expressed MAGE-A4 and only these tumors, regardless of the expression of the other MAGE-A genes. It seems, therefore, that at the level of MAGE gene expression found in tumors, a level clearly lower than that observed in transfected COS cells, only the MAGE-A4 protein can be reliably detected. We conclude that the 57B Mab should be useful for tumor diagnosis related to therapeutic vaccination involving MAGE-A4.
authors.affiliation: Ludwig Institute for Cancer Research, Brussels Branch, Brussels, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Landry
authors.forename: F
authors.initials: F
authors.lastname: Brasseur
authors.forename: G C
authors.initials: GC
authors.lastname: Spagnoli
authors.forename: E
authors.initials: E
authors.lastname: Marbaix
authors.forename: T
authors.initials: T
authors.lastname: Boon
authors.forename: P
authors.initials: P
authors.lastname: Coulie
authors.forename: D
authors.initials: D
authors.lastname: Godelaine
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D019556:COS Cells; D006801:Humans; D007150:Immunohistochemistry; D009369:Neoplasms / Q000737:chemistry*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm