title: Surfactant treatment effects on lung structure and type II cells of preterm ventilated lambs.
abstract: We evaluated surfactant treatment effects on lung morphology and alveolar type II cells of preterm ventilated lambs. Lambs were ventilated for 10 h following treatment of the right lung with natural surfactant. Lung parenchyma from the surfactant-treated right and the untreated left lung was compared morphometrically. Mechanical ventilation without surfactant resulted in distention of alveolar ducts accompanied by shallowing and loss of well-defined alveoli without disruption of collagen or elastin fibers. Surfactant treatment almost completely prevented these changes. The percent of normal parenchyma was 82 +/- 7% in surfactant-treated lobes and 26 +/- 5% in the nontreated lobes (p < 0.05). Type II cells became flatter in lungs ventilated without surfactant, and cell shape was preserved by surfactant treatment. The volume densities of lamellar bodies and multivesicular bodies in alveolar type II cells were not changed by surfactant treatment. With or without surfactant treatment, mechanical ventilation was associated with a shift in lamellar body distribution to a smaller size and a decrease in glycogen content of type II cells. Surfactant treatment of the preterm lung prevents alveolar distortion and atelectasis, but does not result in changes in subcellular organelles in immature type II cells.
authors.affiliation: Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Pinkerton
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
authors.forename: L M
authors.initials: LM
authors.lastname: Dillard
authors.forename: A H
authors.initials: AH
authors.lastname: Jobe
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D005865:Gestational Age; D008168:Lung / Q000473:pathology*; D015388:Organelles / Q000473:pathology; D011650:Pulmonary Alveoli / Q000473:pathology; D011663:Pulmonary Surfactants / Q000627:therapeutic use*; D012121:Respiration, Artificial / Q000009:adverse effects*; D012756:Sheep
chemical_list: D011663:Pulmonary Surfactants