title: Estimate of human gene number provided by genome-wide analysis using Tetraodon nigroviridis DNA sequence.
abstract: The number of genes in the human genome is unknown, with estimates ranging from 50,000 to 90,000 (refs 1, 2), and to more than 140,000 according to unpublished sources. We have developed 'Exofish', a procedure based on homology searches, to identify human genes quickly and reliably. This method relies on the sequence of another vertebrate, the pufferfish Tetraodon nigroviridis, to detect conserved sequences with a very low background. Similar to Fugu rubripes, a marine pufferfish proposed by Brenner et al. as a model for genomic studies, T. nigroviridis is a more practical alternative with a genome also eight times more compact than that of human. Many comparisons have been made between F. rubripes and human DNA that demonstrate the potential of comparative genomics using the pufferfish genome. Application of Exofish to the December version of the working draft sequence of the human genome and to Unigene showed that the human genome contains 28,000-34,000 genes, and that Unigene contains less than 40% of the protein-coding fraction of the human genome.
authors.affiliation: Genoscope and CNRS FRE2231, Evry cedex, France.
authors.forename: H
authors.initials: H
authors.lastname: Roest Crollius
authors.forename: O
authors.initials: O
authors.lastname: Jaillon
authors.forename: A
authors.initials: A
authors.lastname: Bernot
authors.forename: C
authors.initials: C
authors.lastname: Dasilva
authors.forename: L
authors.initials: L
authors.lastname: Bouneau
authors.forename: C
authors.initials: C
authors.lastname: Fischer
authors.forename: C
authors.initials: C
authors.lastname: Fizames
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: P
authors.initials: P
authors.lastname: Brottier
authors.forename: F
authors.initials: F
authors.lastname: Quétier
authors.forename: W
authors.initials: W
authors.lastname: Saurin
authors.forename: J
authors.initials: J
authors.lastname: Weissenbach
mesh_terms: D000818:Animals; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D019295:Computational Biology; D017124:Conserved Sequence / Q000235:genetics; D004247:DNA / Q000235:genetics*; D016208:Databases, Factual; D005091:Exons / Q000235:genetics; D005399:Fishes / Q000235:genetics*; D005796:Genes; D015894:Genome, Human; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012984:Software
chemical_list: D004247:DNA