Effect of ligand binding on the flexibility of dihydrofolate reductase as revealed by compressibility.
The partial specific volume, v, and adiabatic compressibility, beta(s), of Escherichia coli dihydrofolate reductase were measured at 30 degrees C in the presence of various ligands (folate, dihydrofolate, tetrahydrofolate, NADPH, NADP, methotrexate, and KCl). Binding of these ligands (binary and ternary complexes) brought about large changes of v (0.734-0.754 cm(3) g(-1)) and beta(s) (6. 6x10(-6)-9.8x10(-6) bar(-1)), keeping a linear relationship between the two parameters. The values of v and beta(s) increased with an increase in internal cavity, V(cav), and a decrease in accessible surface area, ASA, which were calculated from the X-ray crystal structures of the complexes. A large variation of V(cav) relative to ASA by ligand binding suggested that the cavity is a dominant factor and the effect of hydration might be small for the ligand-induced changes of v and beta(s). The beta(s) values of the binary and ternary complexes suggested a characteristic conformational flexibility of the kinetic intermediates in the enzyme reaction coordinate. Comparison of beta(s) with the cavity distribution in the crystal structures revealed that the flexibility of the intermediates was mainly determined by the total cavity volume with minor contributions of the number, position, and size of cavities. These results demonstrate that the compressibility is a useful measure of the conformational flexibility of the intermediates in the enzyme reaction and that the combined study of compressibility and X-ray crystallography gives new insight into the protein dynamics through the behavior of the cavities.
Kamiyama
T
T
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 739-8526, Higashi-Hiroshima, Japan.
Gekko
K
K
D004926:Escherichia coli / Q000201:enzymology*; D008024:Ligands; D015394:Molecular Structure; D011487:Protein Conformation; D013499:Surface Properties; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry*; D014961:X-Ray Diffraction
D008024:Ligands; D013762:Tetrahydrofolate Dehydrogenase