Detection of a human parvovirus intrauterine infection with the polymerase chain reaction.
OBJECTIVE
To detect human parvovirus B19 intrauterine infection in pregnancy with the polymerase chain reaction (PCR).


METHODS
DNA of chorionic villi and amniotic fluid was extracted and the gene of human parvovirus B19 amplified with PCR.


RESULTS
The study analyzed 61 specimens of chorionic villi and 26 specimens of amniotic fluid and found two positive specimens of chorionic villi and 1 positive specimen of amniotic fluid.


CONCLUSIONS
The vertical transmission of human parvovirus B19 infection in early pregnancy may be a pathway of intrauterine infection. Chorionic villus sampling in early pregnancy and PCR could be developed as a method of prenatal diagnosis of human parvovirus B19 intrauterine infection.
Dong
Z W
ZW
Department of Molecular Biology, National Research Institute for Family Planning, Beijing, People's Republic of China. zwdong@ihw.com.cn
Zhou
S Y
SY
Li
Y
Y
Liu
R M
RM
D000328:Adult; D000653:Amniotic Fluid / Q000821:virology; D002824:Chorionic Villi / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D010322:Parvoviridae Infections / Q000635:transmission / Q000821:virology*; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000592:standards*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D011296:Prenatal Diagnosis / Q000592:standards*; D014591:Uterine Diseases / Q000821:virology*
D017931:DNA Primers; D004279:DNA, Viral