title: Antimicrobial activity of cetylpyridinium chloride washes against pathogenic bacteria on beef surfaces.
abstract: Cetylpyridinium chloride (CPC), a water-soluble, neutral pH, colorless compound, is widely used in oral hygiene products to inhibit bacteria responsible for plaque. Previously, researchers have demonstrated that CPC not only reduces Salmonella typhimurium on poultry but also prevents cross-contamination. To determine the effectiveness of CPC against pathogens associated with lean and adipose beef surfaces, several spray-washing experiments (862 kPa, 15 s, 35 degrees C) with 1% (wt/vol) CPC were conducted. On lean beef surfaces, CPC immediately reduced 5 to 6 log10 CFU/cm2 of Escherichia coli O157:H7 and Salmonella typhimurium to virtually undetectable levels (0 log10 CFU/cm2), as well as after 35 days of refrigerated (4 degrees C), vacuum-packaged storage. On adipose beef surfaces, 5 log10 CFU/cm2 Salmonella typhimurium and E. coli O157:H7 were reduced immediately (>2.5 log10 CFU/cm2) with 1% CPC; by day 35 the reduction was <1.3 log10 CFU/cm2. Further plate overlay analyses indicated that the effectiveness of CPC against pathogens on adipose surfaces was not hampered by the presence of meat components or fatty acids. Additional chemical and microbiological analyses of 1% CPC-treated beef surfaces subjected to a secondary water wash (following contact times of 0, 5, 10, 15, or 30 min) or grinding did reduce pathogenic bacteria and CPC levels. However, residual CPC levels following any of the treatments were considered excessive for human consumption. Despite the residual levels, this study is the first to demonstrate the effect of CPC on pathogenic bacteria associated with beef surfaces immediately after treatment and also after long-term, refrigerated, vacuum-packaged storage.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA. cnc3@psu.edu
authors.forename: C N
authors.initials: CN
authors.lastname: Cutter
authors.forename: W J
authors.initials: WJ
authors.lastname: Dorsa
authors.forename: A
authors.initials: A
authors.lastname: Handie
authors.forename: S
authors.initials: S
authors.lastname: Rodriguez-Morales
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: P J
authors.initials: PJ
authors.lastname: Breen
authors.forename: C M
authors.initials: CM
authors.lastname: Compadre
mesh_terms: D000818:Animals; D002417:Cattle; D002594:Cetylpyridinium / Q000494:pharmacology*; D019453:Escherichia coli O157 / Q000187:drug effects; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D008461:Meat Products / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D002594:Cetylpyridinium