Blood pressure changes after intravenous administration of cell-free hemoglobin A and hemoglobin H in the rat.
Hemoglobin H (HbH) is a tetramer of four beta chains (present in erythrocytes of alpha thalassemia), whereas hemoglobin A is a tetramer of two alpha and two beta chains. Since HbH is known to bind four times more nitric oxide (a vasodilator) at its sulfhydryls compared to HbA, the present studies were conducted to see the effect of HbH and HbA on rat blood pressure. The acute administration (20-2000 nmol/kg) of both HbH and HbA produced a dose-dependent effect on blood pressure. The net change in mean arterial pressure was significantly higher with HbH compared to HbA. Partially nitrosylated (in which SH groups are occupied with NO) HbH retained the property of raising blood pressure to some extent while HbA lost this property. Completely nitrosylated (in which both heme and SH groups are occupied with NO) derivatives of both HbH and HbA reduced the blood pressure to the same extent. The preliminary studies with chronic administration of HbA and HbH resulted in nonsignificant increase in blood pressure. It is concluded that these findings may explain the earlier observations of increased risk of hypertension in individuals with alpha thalassemia.
Reddy
P L
PL
Department of Pathology and Laboratory Medicine, Department of Medicine, Evanston Northwestern Health Care, 2650 Ridge Avenue, Evanston, Illinois, 60201, USA. plreddy@AOL.com
Bowie
L J
LJ
Jiang
H
H
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D006441:Hemoglobin A / Q000008:administration & dosage / Q000494:pharmacology*; D006447:Hemoglobin H / Q000008:administration & dosage / Q000494:pharmacology*; D007262:Infusions, Intravenous; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D006441:Hemoglobin A; D006447:Hemoglobin H