Correlation of genetic polymorphism of cytochrome P4502D6 with dextromethorphan oxidative metabolism in Chinese.
OBJECTIVE
To determine the genetic mechanism of dextromethorphan oxidative polymorphism in Chinese subjects.


METHODS
CYP2D6 genotype was analyzed by polymerase chain reaction (PCR) and Hph I restriction fragment length polymorphism (RFLP) techniques in 119 healthy Chinese subjects.


RESULTS
The gene frequency of CYP2D6*10B was 58.4%, including 13(10.9%) of homozygous wild type(w/w), 33 (27.7%) of homozygous mutant(m/m), and 73(61.3%) of heterozygous genotypes(m/w). Twenty-nine subjects out of 42 intermediate metabolizers(70%) of dextromethorphan were homozygous for CYP2D6*10B. One poor metabolizer subject also showed an m/m genotype. Besides, 10 Chinese subjects were tested and excluded for the presence of any of the six mutant alleles associated with poor metabolism of CYP2D6 in Caucasians.


CONCLUSIONS
The CYP2D6*10B allele containing the C(188)--> T mutation is the major cause of CYP2D6 polymorphism in relation to diminished dextromethorphan oxidative capacity in Chinese subjects.
Cai
W
W
Department of Clinical Pharmacology, Nanjing General Hospital of Nanjing Command, Nanjing, Jiangsu, 210002 P. R. China. wmcai@public1. ptt.js.cn
Chen
B
B
Tao
X
X
Ling
S
S
Zhang
Y
Y
D000293:Adolescent; D000328:Adult; D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics*; D003915:Dextromethorphan / Q000378:metabolism*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D011110:Polymorphism, Genetic
D003915:Dextromethorphan; D019389:Cytochrome P-450 CYP2D6