title: Human antibody response to longus type IV pilus and study of its prevalence among enterotoxigenic Escherichia coli in Bangladesh by using monoclonal antibodies.
abstract: Mouse monoclonal antibodies (MAbs) were derived against longus (CS20), a type IV pilus expressed by human enterotoxigenic Escherichia coli (ETEC). One MAb (ICA39) detected longus in 56 (8.5%) of 662 ETEC isolates obtained from a routine surveillance of diarrheal stools from children and adults. Five patients with diarrhea from whom longus-positive ETEC were isolated were also recruited. Of these 61 isolates, 50 were positive for other colonization factors (CFs; 61% for CFA/II and 21% for CFA/I), and 11 were negative for any of the other 8 CFs that were tested. They were either positive for the heat-stable enterotoxin (ST; n=29) or for the heat-labile enterotoxin (LT) and ST (n=32). All longus-positive ETEC were confirmed by polymerase chain reaction to harbor lngA, the longus structural pilin gene. Sera and/or fecal extracts from the patients reacted with the 22-kDa pilin polypeptide in immunoblots and ELISA. These studies show that longus is prevalent among ETEC in Bangladesh and that longus gives rise to IgA antibody responses in patients.
authors.affiliation: Laboratory Sciences Division, ICDDR, B, Dhaka 1000, Bangladesh. fqadri@icddrb.org
authors.forename: F
authors.initials: F
authors.lastname: Qadri
authors.forename: J A
authors.initials: JA
authors.lastname: Girón
authors.forename: A
authors.initials: A
authors.lastname: Helander
authors.forename: Y A
authors.initials: YA
authors.lastname: Begum
authors.forename: M
authors.initials: M
authors.lastname: Asaduzzaman
authors.forename: J
authors.initials: J
authors.lastname: Xicohténcatl-Cortes
authors.forename: E
authors.initials: E
authors.lastname: Negrete
authors.forename: M J
authors.initials: MJ
authors.lastname: Albert
mesh_terms: D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000032:analysis; D002675:Child, Preschool; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity; D005260:Female; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C022924:colonization factor antigens; D036781:Fimbriae Proteins