The exacerbating effect of insulin-induced hypoglycemia on spontaneous peripheral neuropathy in aged B6C3F1 mice.
The effect of insulin-induced hypoglycemia on spontaneous peripheral neuropathy in aged mice was examined. Ninety-five-week-old female B6C3F1 mice were infused subcutaneously for 2 weeks with 40 or 80 IU/kg/day of insulin with a micro osmotic pump. Blood glucose level was decreased during the infusion (4.3-6.8 mmol/L in mice receiving 40 IU/kg/day of insulin or 2.4-5.4 mmol/L in mice receiving 80 IU/kg/day of insulin versus 6.5-7.6 mmol/L in control mice). In histopathological examination, axonal degeneration and/or remyelination were observed in a small number of nerve fibers of control mice. Similar nerve fiber lesions were observed in mice receiving 40 IU/kg/day of insulin, whereas severer lesions with an increase in segmental axonal degeneration of nerve fibers were observed in 4/7 mice receiving 80 IU/kg/day of insulin. These findings suggest that spontaneous peripheral neuropathy in aged mice is exacerbated by sustained hypoglycemia induced by insulin treatment.
Ikegami
H
H
Safety Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd., Tokyo, Japan.
Tabata
H
H
Matsuzawa
T
T
Suzuki
H
H
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000473:pathology; D001786:Blood Glucose; D001917:Brachial Plexus / Q000187:drug effects* / Q000473:pathology; D020516:Brachial Plexus Neuropathies / Q000097:blood / Q000139:chemically induced* / Q000209:etiology / Q000473:pathology; D002417:Cattle; D016503:Drug Delivery Systems; D005260:Female; D007003:Hypoglycemia / Q000139:chemically induced* / Q000150:complications / Q000473:pathology; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D009410:Nerve Degeneration / Q000139:chemically induced / Q000473:pathology; D009995:Osmosis; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology
D001786:Blood Glucose; D007328:Insulin