Electrospray ionisation-mass spectrometric characterisation of selected anti-psychotic drugs and their detection and determination in human hair samples by liquid chromatography-tandem mass spectrometry.
Electrospray ionisation quadrupole ion-trap mass spectrometric (ESI-MS) characterisation of the anti-psychotic drugs chlorpromazine, trifluoperazine, flupenthixol, risperidone and the antidepressant/internal standard trimipramine is presented and possible mechanisms for the observed MSn fragmentation patterns proposed. A validated liquid chromatography (LC)-MS-MS method is then applied to the detection and determination of these drugs in the hair of a patient under clinical treatment for schizophrenia. Chlorpromazine, trifluoperazine and flupenthixol are identified and determined in this hair sample following alkaline degradation of the matrix, solvent extraction and LC-MS-MS using trimipramine as internal standard.
McClean
S
S
School of ABCS, University of Ulster, Coleraine, Northern Ireland, UK.
O'Kane
E J
EJ
Smyth
W F
WF
D014150:Antipsychotic Agents / Q000032:analysis* / Q000378:metabolism; D002138:Calibration; D002853:Chromatography, Liquid; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D012559:Schizophrenia / Q000378:metabolism; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D014150:Antipsychotic Agents; D007202:Indicators and Reagents