title: Diversification and selection mechanisms for the production of protein repertoires: lessons from the immune system.
abstract: The physiological mechanism for producing antigen-specific antibodies is based on a two-phase neo-Darwinian process: the first phase consists of diversity generation (formation of the repertoire), and the second phase is antigen-mediated selection. In this article, we consider how the natural immunoglobulin gene-diversification processes can be exploited both in vivo and in vitro in order to allow the generation of novel antibody (and heterologous protein) repertoires.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, NSW, Australia. msn@mrc-lmb.cam.ac.uk
authors.forename: M S
authors.initials: MS
authors.lastname: Neuberger
authors.forename: J E
authors.initials: JE
authors.lastname: Sale
authors.forename: S J
authors.initials: SJ
authors.lastname: Cumbers
authors.forename: C J
authors.initials: CJ
authors.lastname: Jolly
authors.forename: M P
authors.initials: MP
authors.lastname: Bemark
authors.forename: M R
authors.initials: MR
authors.lastname: Ehrenstein
authors.forename: A
authors.initials: A
authors.lastname: Lanoue
authors.forename: M
authors.initials: M
authors.lastname: Brüggemann
authors.forename: F D
authors.initials: FD
authors.lastname: Batista
authors.forename: S L
authors.initials: SL
authors.lastname: Davies
authors.forename: G T
authors.initials: GT
authors.lastname: Williams
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000235:genetics; D000915:Antibody Affinity / Q000235:genetics; D000916:Antibody Diversity / Q000235:genetics*; D000918:Antibody Specificity / Q000235:genetics; D002460:Cell Line; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D012641:Selection, Genetic
chemical_list: D000911:Antibodies, Monoclonal