Genomic DNA fingerprinting of Oenococcus oeni strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA-PCR.
Genetic diversity of 60 Oenococcus oeni strains from different wines was evaluated by numerical analysis of (i) pulsed-field gel electrophoresis (PFGE) patterns with endonuclease ApaI and (ii) randomly amplified polymorphic DNA (RAPD)-PCR fingerprints with four oligonucleotide primers. Sixty-two percent of the strains could be distinguished by PFGE, whereas most strains were identified by distinct RAPD-PCR profiles and associated according to the geographical origin. Because of its rapidity and reliability, RAPD-PCR appeared to be a suitable method for typing and monitoring O. oeni strains in winemaking.
Zapparoli
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Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, strada Le Grazie, 37134 Verona, Italy.
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Bordons
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Torriani
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Dellaglio
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D016172:DNA Fingerprinting; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006095:Gram-Positive Cocci / Q000201:enzymology / Q000235:genetics*; D007934:Leuconostoc / Q000201:enzymology / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D014920:Wine / Q000382:microbiology
D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases