title: Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.
abstract: A bacterial toxin that causes progressive distension and death of Chinese hamster ovary (CHO) cells and HeLa cells, termed cytolethal distending toxin (Cdt), has been identified in several diarrhoeagenic bacteria, including Campylobacter spp. (C. jejuni and Cq coli), some pathogenic strains of Escherichia coli and Shigella spp. Genes encoding this toxin were identified as a cluster of three adjacent genes cdtA, cdtB and cdtC. Homologues of cdtB from five species of enterohepatic helicobacters (Helicobacter hepaticus, H. bilis, H. canis and two novel Helicobacter spp. isolated from mice and woodchuck, respectively) were identified by means of degenerative PCR primers, cloned and sequenced. The similarities of these partial cdtB nucleotide sequences from these Helicobacter spp. to those of cdtB genes known to be present in other bacteria were: C. jejuni, 58.3-64.8%; E. coli, 52.3-57.8%, Haemophilus ducreyi, 53.4-58.4% and Actinobacillus actinomycetemcomitans, 52.7-58.1%. Bacterial lysates from four of these helicobacters caused characteristic cytolethal distension of HeLa cells. Cdt caused cell cycle arrest at G2/M phase as measured by flow cytometry. The results demonstrated the presence of a toxin in these Helicobacter spp. belonging to the family of Cdt.
authors.affiliation: *Division of Comparative Medicine and †Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Chien
authors.affiliation: *Division of Comparative Medicine and †Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Nancy S
authors.initials: NS
authors.lastname: Taylor
authors.affiliation: *Division of Comparative Medicine and †Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Ge
authors.affiliation: *Division of Comparative Medicine and †Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Schauer
authors.affiliation: *Division of Comparative Medicine and †Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Vincent B
authors.initials: VB
authors.lastname: Young
authors.affiliation: *Division of Comparative Medicine and †Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: James G
authors.initials: JG
authors.lastname: Fox
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics / Q000633:toxicity; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D005434:Flow Cytometry; D016368:Frameshift Mutation; D016195:G2 Phase / Q000187:drug effects; D006367:HeLa Cells; D016998:Helicobacter / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D008392:Marmota; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; C067044:cytolethal distending toxin