title: Enterobacter cloacae sepsis outbreak in a newborn unit caused by contaminated total parenteral nutrition solution.
abstract: OBJECTIVE
  The study aimed to investigate an outbreak caused by Enterobacter cloacae in a neonate intensive care unit.
  
  
  METHODS
  A descriptive study of an outbreak of sepsis in high-risk neonates was used.
  
  
  METHODS
  The study was set in a tertiary care university teaching hospital.
  
  
  METHODS
  The patients were 11 neonates infected with Enterobacter cloacae whose symptoms and signs of sepsis developed during a 16-hour period. All but one neonate received parenteral nutrition. Isolates from blood cultures, in-use parenteral nutrition solutions, and control aliquots of parenteral nutrition solution were typed by pulsed-field gel electrophoresis.
  
  
  RESULTS
  Enterobacter cloacae was found in the refrigerated aliquots of parenteral nutrition solution, in blood cultures from infected newborns, and from in-use parenteral nutrition solutions. All these strains of Enterobacter cloacae had the same antibiotic susceptibility pattern and the same genomic DNA profile. The strain isolated from the one patient who did not receive parenteral nutrition presented a different susceptibility profile and genotype.
  
  
  CONCLUSIONS
  The source of the nosocomial sepsis was the parenteral nutrition solution in 10 neonates. This contamination apparently occurred during preparation of the parenteral solution.
authors.affiliation: Nosocomial Infection Control Committee, Faculty of Medical Sciences, State University of Campinas, Brazil.
authors.forename: A T
authors.initials: AT
authors.lastname: Tresoldi
authors.forename: M C
authors.initials: MC
authors.lastname: Padoveze
authors.forename: P
authors.initials: P
authors.lastname: Trabasso
authors.forename: J F
authors.initials: JF
authors.lastname: Veiga
authors.forename: S T
authors.initials: ST
authors.lastname: Marba
authors.forename: A
authors.initials: A
authors.lastname: von Nowakonski
authors.forename: M L
authors.initials: ML
authors.lastname: Branchini
mesh_terms: D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D016972:Enterobacter cloacae / Q000235:genetics / Q000302:isolation & purification*; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000209:etiology* / Q000382:microbiology; D005260:Female; D016680:Genome, Bacterial; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D008297:Male; D008826:Microbial Sensitivity Tests; D010289:Parenteral Nutrition, Total / Q000009:adverse effects*; D012307:Risk Factors; D012772:Shock, Septic / Q000209:etiology* / Q000382:microbiology