Development and sexual maturation of Echinococcus vogeli in an alternative definitive host, Mongolian gerbil (Meriones unguiculatus).
An alternative definitive host model for Echinococcus vogeli was developed using Mongolian gerbils. The animals treated with prednisolone tert-butylacetate were orally inoculated with protoscoleces of E. vogeli and the development of worms in the small intestine was observed until 50 days post infection (DPI). The parasites were recovered most frequently from the anterior part of the small intestine. The band formation (the sign of proglottisation) of the worm was observed at 7 DPI, the second proglottid and tests formation at 14 DPI, spermatozoa in seminal receptacle and cleavage of ova in uterus at 21 DPI, the hook formation of oncospheres at 28 DPI and the embryophore formation at 34 DPI. Eggs in the feces were first detected at 35 DPI. The coproantigen detection method developed for E. multilocularis could detect the E. vogeli coproantigen early in the infection, at 7 DPI during the prepatent period. Therefore, this technique could be used for the diagnosis of E. vogeli, replacing ordinary fecal egg examination.
Matsuo
K
K
Laboratory of Parasitology, Graduate School of Veterinary Medicine, Hokkaido University, 060-0818, Sapporo, Japan. kmatsuo@vetmed.hokudai.ac.jp
Shimizu
M
M
Nonaka
N
N
Oku
Y
Y
Kamiya
M
M
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D004195:Disease Models, Animal; D004443:Echinococcosis / Q000469:parasitology*; D004446:Echinococcus / Q000254:growth & development* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005849:Gerbillinae; D007421:Intestine, Small / Q000469:parasitology; D010270:Parasite Egg Count
D000947:Antigens, Helminth