Stereoselective interactions of a specialized antibody with cholesterol and epicholesterol monolayers.
The stereoselective recognition by monoclonal antibodies of two-dimensional monolayers of cholesterol spread at the air-water interface is presented. Using immunofluorescence, we show that one antibody, raised and selected against crystals of cholesterol monohydrate, specifically recognizes monolayers of cholesterol, but not monolayers of epicholesterol--its epimeric form. This demonstrates that stereoselective recognition also applies to protein-surface interactions.
Izhaky
D
D
Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
Addadi
L
L
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D001665:Binding Sites; D002784:Cholesterol / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D008823:Micelles; D013237:Stereoisomerism; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D008823:Micelles; D002784:Cholesterol