Abscissic acid specific expression of RAB18 involves activation of anion channels in Arabidopsis thaliana suspension cells.
The abscissic acid (ABA) transduction cascade following the plasmalemma perception was analyzed in intact Arabidopsis thaliana suspension cells. In response to impermeant ABA, anion currents were activated and K(+) inward rectifying currents were inhibited. Anion current activation was required for the ABA specific expression of RAB18. By contrast, specific inhibition of K(+) channels by tetraethylammonium or Ba(2+) did not affect RAB18 expression. Thus, outer plasmalemma ABA perception triggered two separated signaling pathways.
Ghelis
T
T
Physiologie Cellulaire et Moléculaire des Plantes, UMR CNRS 7632, Université Pierre et Marie Curie (Paris VI), 4 place Jussieu, 75252 Paris, Cedex 05, France.
Dellis
O
O
Jeannette
E
E
Bardat
F
F
Cornel
D
D
Miginiac
E
E
Rona
J P
JP
Sotta
B
B
D000040:Abscisic Acid / Q000494:pharmacology*; D000838:Anions; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins; D017609:Barium Compounds / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004553:Electric Conductivity; D015870:Gene Expression / Q000187:drug effects*; D007473:Ion Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010940:Plant Proteins / Q000235:genetics*; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000502:physiology; D015398:Signal Transduction; D019789:Tetraethylammonium / Q000494:pharmacology; D019287:Zinc Sulfate / Q000494:pharmacology; D020691:rab GTP-Binding Proteins
D000838:Anions; D029681:Arabidopsis Proteins; D017609:Barium Compounds; D002712:Chlorides; D007473:Ion Channels; D010940:Plant Proteins; D026902:Potassium Channel Blockers; D015221:Potassium Channels; C078703:rab18 protein, Arabidopsis; C024986:barium chloride; D019789:Tetraethylammonium; D000040:Abscisic Acid; D019287:Zinc Sulfate; D020691:rab GTP-Binding Proteins