title: Crystallization and preliminary X-ray analysis of high-alkaline pectate lyase.
abstract: Pel-15, a high-alkaline pectate lyase (pectate transeliminase; E.C. 4.2.2.2) from Bacillus sp. strain KSM-P15, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. Two different crystal forms were obtained and preliminary X-ray diffraction data were collected from each crystal form at 100 K. Both forms belong to the orthorhombic space group P2(1)2(1)2(1) and contain one molecule per asymmetric unit. The unit-cell parameters of form I are a = 43.2 (2), b = 60.2 (2), c = 82.2 (2) A and those of form II are a = 42.9 (1), b = 43.4 (1), c = 105.9 (3) A. Diffraction data to a resolution of 1.5 A were collected from form II crystals using a synchrotron-radiation source.
authors.affiliation: Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Akita
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Yamane
mesh_terms: D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols; D011133:Polysaccharide-Lyases / Q000737:chemistry*; D012997:Solvents; D013501:Surface-Active Agents
chemical_list: D001426:Bacterial Proteins; D012997:Solvents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D011133:Polysaccharide-Lyases; C023911:pectate lyase