title: Preparation and characterization of calixarene-coated capillaries for capillary electrophoresis.
abstract: Preparation and characterization of calixarene-coated capillaries for capillary electrophoresis (CE) were exemplified with p-allylcalix[4]arene (pACX4) which was immobilized to the fused silica surface using gamma-methacryloxypropyl-trimethoxysilane (gamma-MAPS) as linking agent. Successful gamma-ACX4 coating was suggested by the greatly decreased electroosmotic flow (EOF), due to the introduction of phenolic hydroxyl groups on the inner surface of the capillaries. A slight slope of EOF versus pH at pH <8 would help make the separation reproducible. The coated columns also featured a low ultraviolet (UV) absorption background and long lifetime (> 6 months at 4<pH<9). Moreover, their special selectivity on phenolic compounds indicated a certain extent of selective interactions between solutes and the calixarene coating, which effected separations partially based on an electrochromatographic mechanism.
authors.affiliation: Center for Molecular Science, Institute of Chemistry, The Chinese Academy of Sciences, Beijing.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: H
authors.initials: H
authors.lastname: Yuan
authors.forename: Z
authors.initials: Z
authors.lastname: Huang
authors.forename: G
authors.initials: G
authors.lastname: Liu
mesh_terms: D047250:Calixarenes; D019075:Electrophoresis, Capillary / Q000379:methods*; D046911:Macromolecular Substances; D015394:Molecular Structure; D018377:Neurotransmitter Agents / Q000302:isolation & purification; D010636:Phenols; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
chemical_list: D046911:Macromolecular Substances; D018377:Neurotransmitter Agents; D010636:Phenols; D047250:Calixarenes