In vivo electron spin resonance assessment of decay constant of nitroxyl radical in selenium-deficient rat.
In vivo reducing capacity and cytosolic glutathione peroxidase (GSH-Px) activity in liver homogenate were evaluated in 6 weeks old Se-deficient and normal rats. GSH-Px was significantly lower in Se-deficient rats than in normal rats. In vivo reducing capacity in head and liver parts, estimated from in vivo signal decay of a nitroxyl spin probe using a low frequency (300 MHz) ESR spectrometer, was significantly decreased in Se-deficient rats, suggesting a decrease of antioxidant capacity in Se-deficient rats.
Matsumoto
K
K
Department of Physical Chemistry, Showa Pharmaceutical University, Machida, Tokyo Japan. knmatsu@ac.shoyaku.ac.jp
Endo
K
K
Utsumi
H
H
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D009589:Nitrogen Oxides / Q000737:chemistry* / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000172:deficiency* / Q000378:metabolism
D009589:Nitrogen Oxides; D005979:Glutathione Peroxidase; C039900:nitroxyl; D012643:Selenium