Spinal CSF from rats with painful peripheral neuropathy evokes catecholamine release from chromaffin cells in vitro.
The environment presented by host tissue may influence cellular transplants in the CNS depending on injury or disease. Here we examined whether chronic pain alters cerebrospinal fluid (CSF), thereby enhancing the analgesic effect of transplanted adrenal cells. CSF samples were taken intracisternally from rats with neuropathic pain induced by chronic constriction injury of the sciatic nerve. The samples were applied to cultured bovine chromaffin-cell clusters while catecholamine release was measured by fast cyclic voltammetry. This caused marked and sustained elevations in catecholamine levels, compared to CSF from sham-operated controls, which were reversible by the nicotinic antagonist mecamylamine. These results suggest that chronic neuropathic pain produces increased CSF levels of secretogogues for chromaffin cells, and illustrates the importance of host microenvironmental factors in determining graft function.
Hentall
I D
ID
The Miami Project to Cure Paralysis, University of Miami School of Medicine, 1600 NW 10th Avenue (R-48), Miami, FL 33136, USA.
Sagen
J
J
D000313:Adrenal Medulla / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D002555:Cerebrospinal Fluid / Q000737:chemistry / Q000378:metabolism; D002556:Cerebrospinal Fluid Proteins / Q000494:pharmacology*; D019439:Chromaffin Cells / Q000187:drug effects* / Q000378:metabolism / Q000637:transplantation*; D002908:Chronic Disease / Q000188:drug therapy; D004594:Electrophysiology; D008297:Male; D008464:Mecamylamine / Q000494:pharmacology; D008839:Microelectrodes; D018733:Nicotinic Antagonists / Q000494:pharmacology; D010146:Pain / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000503:physiopathology*; D010523:Peripheral Nervous System Diseases / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism; D013116:Spinal Cord / Q000737:chemistry / Q000378:metabolism / Q000503:physiopathology*
D002395:Catecholamines; D002556:Cerebrospinal Fluid Proteins; D018733:Nicotinic Antagonists; D011978:Receptors, Nicotinic; D008464:Mecamylamine