Study of the beta1 adrenergic receptor expression in human tissues: immunological approach.
Questions exist regarding tissue distribution of the beta1 adrenergic receptor (beta1-AR). The aim of this study was to investigate relative distribution patterns of the beta1-AR at the protein level in a variety of human tissues by Western blot analysis. The specificity of anti-peptide antibodies was confirmed both by Western blot with recombinant beta1-AR expressed as a membrane protein in E. coli and by immunoprecipitation of membranes from Sf9 cells infected with baculovirus to express the human recombinant beta1-AR. beta1-AR was found in all tissues examined. The relative amount of protein varied significantly between the tissues, from highest in lung and testis to very low in liver. beta1-ARs were rather abundant in heart, kidney, placenta, spleen and thyroid. These results reveal unique distribution of beta1-AR protein that suggests its tissue specific role. Moreover, our data demonstrate a high sensitivity of immunological detection that allows direct comparison of beta1-AR subtype expression and could be used for receptor study in biopsies available in limited amounts, such as human heart biopsy.
Hellgren
I
I
Karolinska Institute at Department of Cardiology, Huddinge University Hospital, Stockholm, Sweden. Irina.Hellgren@medhs.ki.se
Sylvén
C
C
Magnusson
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011817:Rabbits; D018342:Receptors, Adrenergic, beta-1 / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D018342:Receptors, Adrenergic, beta-1; D011994:Recombinant Proteins