[Toxicity of magnetite-dextran particles: morphological study].
Females of OFI mice were given single repeated intravenous injections of magnetite-dextran nanoparticles (MD3), the total partical diameter being 49 nm, with the magnetic core diameter equal to 10-15 nm. MD3 is a superparamagnetic preparation commonly used for magnetic resonance imaging (MRI). The liver, spleen, heart, kidney, and lung microstructures of these mice were determined after MD3 administration. Both dose- and time-dependent changes in the examined organs were compared after single and repeated MD3 doses. MD3 induces an increse in ferritine and iron levels in all the organs, the appearance of small aggregates of lymphoid cells in the liver, the appearance of iron-containing cell formations in hepatic sinusoids, presumably composed of the Kupffer cells and portal macrophages, splenomegaly, and hemostasis of spleen blood vessels. The pronounced morphological alterations have been revealed primarily in the liver and spleen after a single administration of high MD3 doses and after repeated MD3 injections. The results of The present investigation seem to narrow somewhat the safety limits of superparamagnetic iron oxide particles. Nevertheless, the degree of morphological changes in the liver and spleen in our experiments appeared to be rather low even after a single MD3 dose that exceeds approximately by 200 times a dose necessary for diagnostics in MRI.
Okon
E E
EE
Institute of Cytology, Russian Academy of Sciences, St. Petersburg. callpath@link.cytspb.rssi.ru
Pulikan
D
D
Pereverzev
A E
AE
Kudriavtsev
B N
BN
Zhale
P
P
D000818:Animals; D003911:Dextrans / Q000633:toxicity*; D005260:Female; D005293:Ferritins / Q000378:metabolism; D052203:Ferrosoferric Oxide; D006321:Heart / Q000187:drug effects; D007501:Iron / Q000378:metabolism / Q000633:toxicity*; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D051379:Mice; D010087:Oxides / Q000633:toxicity*; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D003911:Dextrans; D010087:Oxides; D005293:Ferritins; D007501:Iron; D052203:Ferrosoferric Oxide
K voprosu o toksichnosti magnetit-dekstranovykh chastits: morfologicheskioe issledovanie.