title: 72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli.
abstract: An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.
authors.affiliation: Institut für Genetik, Universität zu Köln, FRG.
authors.forename: B
authors.initials: B
authors.lastname: von Wilcken-Bergmann
authors.forename: M
authors.initials: M
authors.lastname: Koenen
authors.forename: H W
authors.initials: HW
authors.lastname: Griesser
authors.forename: B
authors.initials: B
authors.lastname: Müller-Hill
mesh_terms: D000595:Amino Acid Sequence; D019677:Artificial Gene Fusion; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic; D009876:Operon; D012097:Repressor Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C050993:Galactose repressor proteins; D012097:Repressor Proteins; D001616:beta-Galactosidase