title: Early life humoral response of ducks to DNA immunization against hepadnavirus large envelope protein.
abstract: DNA vaccination may represent an interesting strategy for early life immunization. However, in some cases, this approach has been shown to induce a tolerance rather than immunity. We have compared the efficiency of neonatal DNA or protein immunization against hepadnavirus envelope protein using the duck hepatitis B virus (DHBV) model. Three-day-old ducklings were immunized with either a plasmid encoding the DHBV pre-S/S large envelope protein (L), or a recombinant preS protein, followed by sequential DNA or protein boosts at weeks 4 and 15. Our results showed that genetic immunization of duck neonates induced specific humoral response to DHBV L protein. Interestingly, an enhanced antibody response was elicited when animals received DNA priming-DNA boosting as compared to DNA priming-protein boosting.
authors.affiliation: Unité de Recherche sur les Virus des Hépatites et les Pathologies Associées, Institut National de la Santé et de la Recherche Médicale unité 271, Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Rollier
authors.forename: C
authors.initials: C
authors.lastname: Charollois
authors.forename: C
authors.initials: C
authors.lastname: Jamard
authors.forename: C
authors.initials: C
authors.lastname: Trepo
authors.forename: L
authors.initials: L
authors.lastname: Cova
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D004372:Ducks; D006508:Hepatitis Antibodies / Q000097:blood*; D015611:Hepatitis B Virus, Duck / Q000276:immunology*; D007114:Immunization; D007115:Immunization Schedule; D019444:Vaccines, DNA / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D006508:Hepatitis Antibodies; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014765:Viral Vaccines