title: The inhibitor of apoptosis, cIAP2, functions as a ubiquitin-protein ligase and promotes in vitro monoubiquitination of caspases 3 and 7.
abstract: The inhibitor of apoptosis, cIAP2, contains a putative Ring finger motif at the C terminus. Using in vitro ubiquitination assays, we found that the Ring finger of cIAP2 alone possesses intrinsic ubiquitin ligase activity and promotes substrate-independent ubiquitination. It also promotes ubiquitination of caspases 3 and 7 but not caspase-1. The Ring fingers of c-Cbl and Apc11 failed to promote caspase-7 ubiquitination, suggesting that the Ring finger of cIAP2 itself is involved in substrate recognition.
authors.affiliation: Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies and the Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: H k
authors.initials: Hk
authors.lastname: Huang
authors.forename: C A
authors.initials: CA
authors.lastname: Joazeiro
authors.forename: E
authors.initials: E
authors.lastname: Bonfoco
authors.forename: S
authors.initials: S
authors.lastname: Kamada
authors.forename: J D
authors.initials: JD
authors.lastname: Leverson
authors.forename: T
authors.initials: T
authors.lastname: Hunter
mesh_terms: D000595:Amino Acid Sequence; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases / Q000378:metabolism*; D051034:Inhibitor of Apoptosis Proteins; D008025:Ligases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D014452:Ubiquitins; D014764:Viral Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus; D044767:Ubiquitin-Protein Ligases; D053148:Caspase 3; D053179:Caspase 7; D020169:Caspases; D008025:Ligases