Cytocidal and apoptotic effects of the ClyA protein from Escherichia coli on primary and cultured monocytes and macrophages.
Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates a hemolytic phenotype. We show here that highly purified ClyA and ClyA-expressing E. coli were cytotoxic and apoptogenic to fresh as well as cultured human and murine monocytes/macrophages.
Lai
X H
XH
Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden.
Arencibia
I
I
Johansson
A
A
Wai
S N
SN
Oscarsson
J
J
Kalfas
S
S
Sundqvist
K G
KG
Mizunoe
Y
Y
Sjöstedt
A
A
Uhlin
B E
BE
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D053938:DNA Fragmentation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000473:pathology; D020298:U937 Cells
D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C119944:hlyE protein, E coli