Self-assembling photosynthetic reaction centers on electrodes for current generation.
Photosynthetic reaction centers (RCs) made from photosynthetic organisms can be used in solar batteries because their molecules cause light-induced charge separation. We present a simple immobilization system of the intact RCs from Rhodobacter sphaeroides on an electrode that uses nickel ligand binding by the hexameric histidine tag on H subunit (HHisRC). The binding constant of HHisRC to the nickel-nitrilotriacetic acid (Ni-NTA) chip measured with a surface plasmon resonance instrument was 1.6 x 10(8) M-1. HHisRCs were immobilized on an indium tin oxide electrode overlaid with an Ni-NTA gold substrate. The photoinduced displacement current of this electrode was measured to estimate the orientation of HHisRC on the electrode, and the detachability of HHisRC from the electrode was determined by using an imidazole solution wash. The direction of the flash-light-induced displacement current suggested that the H subunit side of the immobilized HHisRC faced the surface of the electrode. The photoinduced current disappeared after the electrode was washed in the imidazole solution. This simple immobilization and detachment of HHisRC to the electrode might be useful for making a reproducible photocurrent device.
Nakamura
C
C
National Institute for Advanced Interdisciplinary Research, AIST, MITI, Ibaraki, Japan. nakamura@nair.go.jp
Hasegawa
M
M
Yasuda
Y
Y
Miyake
J
J
D015374:Biosensing Techniques; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D009532:Nickel; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives; D009942:Organometallic Compounds; D045322:Photosynthetic Reaction Center Complex Proteins; D012242:Rhodobacter sphaeroides; D020349:Surface Plasmon Resonance
D009942:Organometallic Compounds; D045322:Photosynthetic Reaction Center Complex Proteins; C088321:nickel nitrilotriacetic acid; D009532:Nickel; D009571:Nitrilotriacetic Acid