Determination of the binding parameters of drug to protein by equilibrium dialysis/piezoelectric quartz crystal sensor.
A novel method, equilibrium dialysis/piezoelectric quartz crystal sensor, applied to determine the binding parameters of diethyldithiocarbamate to human plasma protein is proposed. Based on the investigation of the equilibrium reaction for the binding of drug to protein, the related theoretical equations for this binding were derived. By monitoring the frequency responses of a copper-plated piezoelectric quartz crystal sensor to drug in and out of a dialysis membrane after equilibrium, the binding parameters were determined, i.e., 0.375 micromol g(-1) for beta(p), 6.496 microM for K(dp), 141.99 L mmol(-1) for K(p), and 0.043 for N. These values were in good agreement with reference values. It was found that this method may have application for studying the characteristics of the interaction between other drugs and proteins.
Zhou
A
A
College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, People's Republic of China.
He
D
D
Nie
L H
LH
Yao
S Z
SZ
D015374:Biosensing Techniques; D001798:Blood Proteins / Q000378:metabolism; D002614:Chelating Agents / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003300:Copper; D004050:Ditiocarb / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D017551:Microdialysis / Q000379:methods*; D015233:Models, Statistical; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism
D001798:Blood Proteins; D002614:Chelating Agents; D004364:Pharmaceutical Preparations; D012709:Serum Albumin; D003300:Copper; D004050:Ditiocarb