title: Cryptic and exposed invariable regions of VlsE, the variable surface antigen of Borrelia burgdorferi sl.
abstract: Borrelia burgdorferi, the Lyme disease spirochete, possesses a surface protein, VlsE, which undergoes antigenic variation. VlsE contains two invariable domains and a variable one that includes six variable and six invariable regions (IRs). Five of the IRs are conserved among strains and genospecies of B. burgdorferi sensu lato. IR(6) is conserved, immunodominant, and exposed at the VlsE surface but not at the spirochete surface, as assessed in vitro. In the present study, the remaining conserved IRs (IR(2) to IR(5)) were investigated. Antisera to synthetic peptides based on each of the IR(2) to IR(5) sequences were produced in rabbits. Antipeptide antibody titers were similarly high in all antisera. Native VlsE was immunoprecipitable with antibodies to IR(2), IR(4), and IR(5) but not to IR(3), indicating that the first three sequences were exposed at the VlsE surface. However, negative surface immunofluorescence and in vitro antibody-mediated killing results indicated that none of the IRs were accessible to antibody at the spirochetal surface in vitro.
authors.affiliation: Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433, USA.
authors.forename: F T
authors.initials: FT
authors.lastname: Liang
authors.forename: J M
authors.initials: JM
authors.lastname: Nowling
authors.forename: M T
authors.initials: MT
authors.lastname: Philipp
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D000954:Antigens, Surface / Q000737:chemistry* / Q000276:immunology*; D001426:Bacterial Proteins; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008074:Lipoproteins / Q000737:chemistry* / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011233:Precipitin Tests; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D008074:Lipoproteins; D010455:Peptides; C105546:VlsE protein, Borrelia burgdorferi