Identification of seven genes in the major histocompatibility complex class I region of the zebrafish.
Physical linkage of genes whose products are involved in similar physiological pathways may have functional significance. The identification of conserved gene linkage in distantly related organisms can therefore strengthen the hypothesis of selection acting towards keeping genes on a chromosome. We used the cDNA selection technique and the polymerase chain reaction (PCR) with generic primers for the identification of new genes on the genomic clones bearing the major histocompatibility complex (Mhc) class I genes of the zebrafish (Danio rerio). We found six new genes (BING1, DAXX, TAPBP, KNSL2, TAP2B and KE6) whose orthologues are known to be linked to the Mhc class II region in humans and mice. In addition, a new zebrafish Mhc class I gene, termed Dare-UFA, was detected. By contrast, a search for the human leucocyte antigen (HLA)-linked BING3, KE3 and SACM2L genes revealed that these loci are not located on the class I clones of the zebrafish. The zebrafish class I region contains repetitive elements with similarity to the DANA, SATA and LINE repeats, as well as Tc1 transposable elements. Our findings indicate a high degree of linkage conservation between the zebrafish class I and the mammalian class II regions.
Sültmann
H
H
Max-Planck-Institut für Biologie, Abteilung Immungenetik, Tübingen, Germany.
Murray
B W
BW
Klein
J
J
D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D017920:Antiporters / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification; D056970:Co-Repressor Proteins; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification; D005805:Genes, MHC Class I / Q000235:genetics*; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000302:isolation & purification; D047908:Intracellular Signaling Peptides and Proteins; D016547:Kinesins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D026901:Membrane Transport Proteins; D051379:Mice; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification; D015027:Zebrafish / Q000235:genetics* / Q000276:immunology*; D029961:Zebrafish Proteins
D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D048868:Adaptor Proteins, Signal Transducing; D017920:Antiporters; D002352:Carrier Proteins; D056970:Co-Repressor Proteins; C106985:DAXX protein, human; D004268:DNA-Binding Proteins; C491473:Daxx protein, mouse; C102228:HSD17B8 protein, human; D007136:Immunoglobulins; D047908:Intracellular Signaling Peptides and Proteins; C486567:KIF15 protein, human; D026901:Membrane Transport Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D011506:Proteins; C489516:Tap2 protein, mouse; C484736:ZBTB22 protein, human; C112042:Zbtb22 protein, mouse; D029961:Zebrafish Proteins; C410083:kifc1 protein, zebrafish; C489513:tap2t protein, zebrafish; C107762:tapasin; C484737:zbtb22b protein, zebrafish; C071692:TAP2 protein, human; D010088:Oxidoreductases; D016547:Kinesins