title: A novel approach to the recovery of biologically active oligosaccharides from milk using a combination of enzymatic treatment and nanofiltration.
abstract: A new easily scalable approach to the recovery of biologically active oligosaccharides from milk has been developed which relies on the combination of enzymatic treatment of defatted milk using beta-galactosidase and nanofiltration. It was shown that enzymatic hydrolysis of lactose significantly improves the efficiency and selectivity of membrane-based separations. With the best membrane, as much as 6.7 g of oligosaccharides (containing very little contaminating lactose) could be obtained from one liter of defatted human milk in just four nanofiltration cycles. The human milk oligosaccharides recovered by this method were shown to inhibit binding of intimin, an adhesion molecule of enteropathogenic Escherichia coli, to epithelial cells in vitro. No significant difference in the oligosaccharide profile between samples prepared by this method and conventional gel-permeation chromatography was found. The developed approach is also suitable for the recovery of substantial quantities of tri- and tetra-saccharides from caprine milk.
authors.affiliation: Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, United Kingdom.
authors.forename: D B
authors.initials: DB
authors.lastname: Sarney
authors.forename: C
authors.initials: C
authors.lastname: Hale
authors.forename: G
authors.initials: G
authors.lastname: Frankel
authors.forename: E N
authors.initials: EN
authors.lastname: Vulfson
mesh_terms: D018829:Adhesins, Bacterial; D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D002352:Carrier Proteins; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000378:metabolism; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005260:Female; D006041:Goats; D006801:Humans; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D008895:Milk, Human / Q000737:chemistry / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification*; D011485:Protein Binding; D014462:Ultrafiltration; D001616:beta-Galactosidase / Q000494:pharmacology*
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D009844:Oligosaccharides; C073819:eaeA protein, E coli; D001616:beta-Galactosidase