title: Apoptosis in human Jurkat T cells after culture with live Taenia crassiceps cysticerci in vitro.
abstract: This study examines the effects of Taenia crassiceps cysticerci on the viability of a human T lymphocyte cell line (Jurkat). Both budding and non-budding T. crassiceps metacestodes were cultured over 24 and 48 h in the presence of Jurkat cells. Cell viability decreased with increasing numbers of cysticerci, particularly budding cysticerci. Single cell gel electrophoresis (comet) analysis, which grades DNA damage, showed a significant increase in apoptosis at 24 and 48 h. The morphology of treated cells was determined using acridine orange with the classical morphology of apoptotic bodies seen to increase with increasing cysticerci numbers over time. These results indicate that parasite-induced apoptosis occurs during murine cysticercosis. Such a mechanism may be important in survival of other metacestode infections of medical or veterinary importance.
authors.affiliation: Department of Biological Sciences, University of Salford, UK.
authors.forename: K M
authors.initials: KM
authors.lastname: O'Connell
authors.forename: M T
authors.initials: MT
authors.lastname: Rogan
mesh_terms: D000165:Acridine Orange / Q000737:chemistry; D000818:Animals; D017209:Apoptosis; D002470:Cell Survival; D004396:Coloring Agents / Q000737:chemistry; D020552:Comet Assay; D004249:DNA Damage; D005047:Etoposide / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D019169:Jurkat Cells / Q000737:chemistry / Q000166:cytology / Q000469:parasitology; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors / Q000737:chemistry; D018709:Statistics, Nonparametric; D013621:Taenia / Q000254:growth & development*; D013622:Taeniasis / Q000469:parasitology*; D014343:Trypan Blue / Q000737:chemistry
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D019384:Nucleic Acid Synthesis Inhibitors; D005047:Etoposide; D000165:Acridine Orange; D014343:Trypan Blue