title: Differential expression of two CYP1A genes in rainbow trout (Oncorhynchys mykiss).
abstract: Differential expression of two rainbow trout CYP1A genes was measured in vivo and in vitro in response to treatment with the model CYP1A inducers beta-naphthoflavone (BNF), 3-methylcholanthrene (3-MC), isosafrole (ISF), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, only in vitro). Originally described by Berndtson and Chen (Arch. Biochem. Biophys. 310, 187-195, 1994) as CYP1A1 and CYP1A2, these genes were renamed CYP1A3 and CYP1A1, respectively, by the P450 nomenclature committee. A significant, differential, inducer-dependent induction of the two CYP1A mRNAs, as measured by RNase protection assay, was observed in vivo. CYP1A3 and CYP1A1 mRNA levels in liver were significantly induced 50- and 18-fold, respectively, following ip injection with BNF. Conversely, CYP1A3 and CYP1A1 mRNA levels were significantly induced 5- and 66-fold, respectively, following ip injection with 3-MC. Isosafrole had no significant effect on in vivo induction of CYP1A mRNA levels. In primary cultures of hepatocytes, BNF, 3-MC, ISF, as well as TCDD all significantly induced CYP1A3 and CYP1A1 mRNA levels compared to controls. The differential induction of the two CYP1A genes was not as evident in vitro as in vivo. In addition, reanalysis and sequence comparison of the these two trout CYP1A genes with the first trout CYP1A cDNA described by Heilmann et al. (DNA 7, 379-387, 1988) indicate that the Heilmann cDNA is a hybrid of the two trout genes. The 5' portion of the cDNA sequence (212 bp) was determined by sequencing of a genomic clone and is 100% identical to the trout CYP1A3 gene. The majority of the cDNA sequence (2377 bp), however, was sequenced from a partial cDNA clone and is 99.2% identical to trout CYP1A1. Although the nomenclature of these two trout CYP1A genes is undergoing revision, these results demonstrate a differential, inducer-dependent response to model mammalian CYP1A inducers.
authors.affiliation: Department of Pathology, Program in Toxicology, University of Maryland at Baltimore, 10 South Pine Street, Baltimore, Maryland 21201, USA. christina.rabergh@utu.fi
authors.forename: C M
authors.initials: CM
authors.lastname: Råbergh
authors.forename: N H
authors.initials: NH
authors.lastname: Vrolijk
authors.forename: M M
authors.initials: MM
authors.lastname: Lipsky
authors.forename: T T
authors.initials: TT
authors.lastname: Chen
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D015152:Blotting, Northern; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D004790:Enzyme Induction; D005260:Female; D005786:Gene Expression Regulation; D008099:Liver / Q000166:cytology / Q000201:enzymology; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D017686:Oncorhynchus mykiss / Q000235:genetics*; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012451:Safrole / Q000494:pharmacology; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D019324:beta-Naphthoflavone / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D008748:Methylcholanthrene; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D019363:Cytochrome P-450 CYP1A1; C106496:cytochrome P-450 CYP1A3 (rainbow trout); D012451:Safrole; C015959:isosafrole