title: A polymorphism upstream from the human paraoxonase (PON1) gene and its association with PON1 expression.
abstract: Human serum paraoxonase (PON1) is an esterase that has been shown to decrease the susceptibility of lipoproteins to lipid peroxidation. We found a polymorphism of cytosine/thymidine (-108C/T, the number is from the ATG codon) in the upstream region of the PON1 gene. The luciferase activity was lower in the -108T allele than in the -108C allele. The serum PON1 concentrations in 132 normal subjects were as follows: -108CC>-108CT and>-108TT genotypes. The polymorphism upstream from the PON1 gene is associated with transcription of the PON1 gene and the serum PON1 concentration.
authors.affiliation: The Second Department of Internal Medicine, Kochi Medical School, Kohasu, Okoh-cho, Nankoku, 781-8505, Kochi, Japan. suehirot@kochi-ms.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Suehiro
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Shiinoki
authors.forename: Y
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Kumon
authors.forename: M
authors.initials: M
authors.lastname: Shindo
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
mesh_terms: D000483:Alleles; D043303:Aryldialkylphosphatase; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003327:Coronary Disease / Q000097:blood / Q000201:enzymology / Q000235:genetics; D004247:DNA / Q000032:analysis*; D017931:DNA Primers / Q000737:chemistry; D004950:Esterases / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D015227:Lipid Peroxidation / Q000235:genetics*; D008156:Luciferases / Q000097:blood; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA; D008156:Luciferases; D004950:Esterases; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human