title: Cellular distribution of isozymes of protein kinase C in septal olfactory epithelium of mice.
abstract: To determine the presence of protein kinase C (PKC) isozymes in the septal olfactory epithelium of mice (mSOE), western blotting and immunohistochemistry were performed using antibodies against PKC isozymes. With the exception of PKC-betaI, all of the PKC isozymes were detected in the whole lysate of septal tissue layer and apparent molecular weights for each isoform were found. PKC-alpha, PKC-gamma and PKC-epsilon were detected in the olfactory glandular cells of the lamina propria, and PKC-betaI and PKC-betaII were located in the microvillar cells. Neither novel PKC nor atypical PKC was detected in olfactory glandular cells or microvillar cells, except for PKC-epsilon. PKC-lambda was localized in the mucous layer of the mSOE. Meanwhile, PKC-delta and PKC-xi were distributed in the receptor cells in the mSOE. These data demonstrate the isoform-specific expression of PKC in mSOE and suggest a role for the novel and atypical types of PKC in olfactory transduction.
authors.affiliation: Department of Biology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
authors.forename: W S
authors.initials: WS
authors.lastname: Kang
authors.forename: W K
authors.initials: WK
authors.lastname: Lee
authors.forename: M S
authors.initials: MS
authors.lastname: Seo
authors.forename: J K
authors.initials: JK
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Han
authors.forename: Y W
authors.initials: YW
authors.lastname: Moon
authors.forename: K K
authors.initials: KK
authors.lastname: Cho
authors.forename: M J
authors.initials: MJ
authors.lastname: Kim
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: Y H
authors.initials: YH
authors.lastname: Jo
authors.forename: C R
authors.initials: CR
authors.lastname: Choi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009300:Nasal Septum / Q000201:enzymology; D009475:Neurons, Afferent / Q000201:enzymology; D009831:Olfactory Mucosa / Q000201:enzymology*; D011493:Protein Kinase C / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C