Identification, by cDNA microarray, of A-raf and proliferating cell nuclear antigen as genes induced in rat lung by exposure to diesel exhaust.
Diesel exhaust particles (DEP) contain various carcinogens and mutagens, and chronic exposure to diesel exhaust (DE) induces pulmonary cancer in experimental animals. However, the oncogenes involved in pulmonary carcinogenesis have not been identified. After F344 rats were exposed to DE containing 6 mg/m3 DEP for 4 weeks, oncogenes and related genes expressed in their lungs were surveyed using a new technique, cDNA microarray, and the results were confirmed by northern blot analysis. Expression of A-raf and proliferating cell nuclear antigen (PCNA) mRNAs was induced in rat lung by exposure to DE. These results suggest that A-raf and PCNA might contribute to pulmonary carcinogenesis in rats.
Sato
H
H
Environmental Health Sciences Division, National Institute for Environmental Studies, Ibaraki, Japan.
Sagai
M
M
Suzuki
K T
KT
Aoki
Y
Y
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000187:drug effects*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics*; D051381:Rats; D011916:Rats, Inbred F344; D001335:Vehicle Emissions / Q000633:toxicity*
D017931:DNA Primers; D018076:DNA, Complementary; D018809:Proliferating Cell Nuclear Antigen; D001335:Vehicle Emissions; D019908:Proto-Oncogene Proteins c-raf