Expansion of transgenic tobacco protoplasts expressing pumpkin ascorbate oxidase is more rapid than that of wild-type protoplasts.
When pumpkin (Cucurbita spp., cv. Ebisu Nankin) ascorbate oxidase cDNA was introduced into cultured cells of tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow No. 2) by Agrobacterium-mediated transformation, the transgenic cells expressed and secreted the recombinant pumpkin ascorbate oxidase into the culture medium. These transgenic cells showed no morphological difference from wild-type cells. However, in the presence of applied hormones protoplasts prepared from the transgenic cells elongated more rapidly than those of wild-type cells. We propose that ascorbate oxidase may play a key role in the regulation of cell expansion perhaps by controlling transport processes through the plasma membrane, but not by affecting the cell wall.
Kato
N
N
Faculty of Applied Biological Science, Hiroshima University, Japan.
Esaka
M
M
D001204:Ascorbate Oxidase / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D019662:Cucurbitaceae / Q000235:genetics; D015151:Immunoblotting; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins; D001204:Ascorbate Oxidase