Fabrication of an electrode-viologen-hydrogenase heterogeneous system and the electrochemical hydrogen evolution.
An indium tin oxide (ITO) electrode was chemically modified by one layer of viologen (VIO) derivative, which possessed a persistent and reproducible electrochemical response. A monolayer of a thermal stable hydrogenase from Thiocapsa roseopersicina was stabilized on a synthesized poly-L-lysine subphase surface and transferred onto the electrode for fabrication of an ITO-VIO-hydrogenase heterogeneous system. Electrochemical properties of both the ITO-VIO monolayer and the heterogeneous ITO-VIO-hydrogenase system have been investigated. Hydrogen evolution could be measured by potentiostating the VIO-hydrogenase-covered ITO electrode to "electroplate" [(VIO+)n]surf, and a large increase in hydrogen evolution was observed when using an electrolyte solution containing sodium dithionite. We discuss the possible electron transfer process.
Qian
D J
DJ
National Institute of Advanced Interdisciplinary Research, AIST, Ibaraki, Japan.
Nakamura
C
C
Noda
K
K
Zorin
N A
NA
Miyake
J
J
D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006859:Hydrogen; D006864:Hydrogenase / Q000378:metabolism*; D011199:Potentiometry; D020586:Thiocapsa / Q000201:enzymology; D017971:Tin Compounds
D004800:Enzymes, Immobilized; D017971:Tin Compounds; C109984:indium tin oxide; D006859:Hydrogen; D006864:Hydrogenase