Effect of calmodulin antagonists on the compound action potential of the cochlea.
This study aimed to evaluate the effect of calmodulin antagonists on the threshold of the compound action potential (CAP) and the functional recovery of the cochlea after transient ischemia. When trifluoperazine and W-7 were administered to albino guinea pigs with perilymphatic perfusion, these drugs did not significantly affect the CAP thresholds. Transient cochlear ischemia of 30-min duration was obtained via a skull base approach. Although trifluoperazine significantly ameliorated the post-ischemic CAP threshold shifts 4 h after the onset of reperfusion, 1 to 50 microM W-7 did not affect the CAP threshold shifts. These results suggest that the action antagonizing calmodulin has no effect on the CAP threshold, while the role that calmodulin plays in cochlear ischemia-reperfusion injury still remains unclear.
Tabuchi
K
K
Department of Otolaryngology, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, 305-8575, Tsukuba, Japan.
Tsuji
S
S
Hara
A
A
Kusakari
J
J
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D003051:Cochlea / Q000098:blood supply / Q000187:drug effects* / Q000502:physiology*; D004056:Differential Threshold / Q000187:drug effects; D006168:Guinea Pigs; D007511:Ischemia / Q000503:physiopathology; D010477:Perfusion; D010498:Perilymph; D020127:Recovery of Function; D012039:Regional Blood Flow / Q000502:physiology; D015424:Reperfusion; D013449:Sulfonamides / Q000008:administration & dosage / Q000494:pharmacology; D014268:Trifluoperazine / Q000008:administration & dosage / Q000494:pharmacology
D002147:Calmodulin; D013449:Sulfonamides; D014268:Trifluoperazine; C017967:W 7