Chemical inhibitors: a tool for plant cell cycle studies.
Synchrony provides a large number of cells at defined points of the cell cycle. Highly synchronised cells are powerful and effective tools for molecular analyses and for studying the biochemical events of the cell cycle in plants. Usually, plant cell suspensions can be synchronised by chemical agents, which arrest the cell cycle by acting on the driving forces of the cell cycle engine such as cyclin-dependent kinase activity, enzymes involved in DNA synthesis or proteolysis of cell cycle regulators or by acting on the cell cycle apparatus (mitotic spindle). The specificity, reversibility and efficiency of each type of cell cycle inhibitor are described and related to their mode of action.
Planchais
S
S
Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1ZT, UK. sp292@biotech.cam.ac.uk
Glab
N
N
Inzé
D
D
Bergounioux
C
C
D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D059828:Plant Cells; D018521:Plant Physiological Phenomena
D004791:Enzyme Inhibitors; D018844:Cyclin-Dependent Kinases