Mutation detection in the breast cancer gene BRCA1 using the protein truncation test.
About 400 distinct mutations have been defined in the BRCA1 gene, and these are spread fairly evenly through the 5592 bp of coding DNA. This circumstance presents a formidable challenge for mutation screening. Apart from total direct sequencing, the preferred screening method has been single-strand conformation polymorphism (SSCP) gels, with a smaller input from constant denaturant gradient electrophoresis (CDGE), heteroduplex (HD) analysis, and mismatch cleavage. The protein truncation test (PTT) was used early in BRCA1 mutation screening but has not been widely adopted, perhaps because a straightforward analysis of the whole BRCA1 gene requires working with RNA and all its perceived problems. The present work was undertaken to assess the practicality of using the PTT under routine conditions for the screening of long genes such as BRCA1 that are not highly expressed in lymphocytes. We conclude that, provided RNA preparation is carried out effectively and consistently, the PTT approach has significant advantages over other methodologies such as SSCP gels.
Moore
W
W
Biophysics Laboratories, University of Portsmouth, UK.
Bogdarina
I
I
Patel
U A
UA
Perry
M
M
Crane-Robinson
C
C
D001483:Base Sequence; D015331:Cohort Studies; D017931:DNA Primers; D005091:Exons; D019398:Genes, BRCA1; D006801:Humans; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational; D014176:Protein Biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers