Applications of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones.
Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges of detectable antigens and enabled the observation of antigen-antigen or mRNA-antigen combinations. This review article deals with technical aspects, procedures, and applications in endocrine cells.
Osamura
R Y
RY
Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan. osamura@is.icc.u-tokai.ac.jp
Itoh
Y
Y
Matsuno
A
A
D000180:Acrylic Resins; D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism; D004703:Endocrine System / Q000166:cytology / Q000378:metabolism; D006728:Hormones / Q000096:biosynthesis* / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods; D010452:Peptide Biosynthesis; D010455:Peptides / Q000378:metabolism*; D016613:Plastic Embedding
D000180:Acrylic Resins; D006728:Hormones; D010455:Peptides; C048707:LR white