title: [Radiation-induced apoptosis and p53, bcl-2 gene expression products in QGY-7703 cell line in vitro].
abstract: OBJECTIVE
  To investigate the dose response and time course of radiation-induced apoptosis and gene expression products of p53, bcl-2 genes in human hepatocarcinoma cell line QGY-7703 in vitro.
  
  
  METHODS
  Apoptosis was induced by radiation and quantitated by Flow Cytometry. The oncoproteins of p53 and bcl-2 genes after radiation were detected immunocytochemically on cell cytospinned slides. The data were analyzed with Sony Mias-300 Spato Immage Analyzer.
  
  
  RESULTS
  The percentage of apoptosis in unradiation QGY-7703 was 4. 79%. After radiation, the apoptotic fraction increased with incubation time and radiation dose. Both gene expression products increased with incubation time and reached plateau at 6h post-radiation.
  
  
  CONCLUSIONS
  After radiation, the response and time course of radiation-induced apoptosis in QGY-7703 cell line are similar to those of p53, bcl-2 oncoproteins. The later proceeds by the apoptosis peak about 6 h. The results suggest that p53 and bcl-2 genes are the regulating factors partially in radiation-induced apoptosis of QGY-7703 cell line.
authors.affiliation: Department of Radiotherapy, First Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: He
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy*; D004307:Dose-Response Relationship, Radiation; D019254:Genes, bcl-2; D016158:Genes, p53; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology / Q000532:radiotherapy*; D014407:Tumor Cells, Cultured