Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium.
Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16,525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21.
Fliegerova
K
K
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 10-Uhrineves, Prague, 104 00, Czech Republic.
Pazoutova
S
S
Pristas
P
P
Flint
H J
HJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D020357:Selenomonas / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
D004269:DNA, Bacterial