title: Molecular cloning and characterization of CRLM-2, a novel type I cytokine receptor preferentially expressed in hematopoietic cells.
abstract: A murine expressed sequence tag (EST) showing homology with erythropoietin receptor (EPOR) was identified in the EST database. Cloning of the full-length cDNA revealed a 359 amino acid novel type I cytokine receptor, designated cytokine receptor like molecule-2 (CRLM-2). While CRLM-2 lacks typical WSXWS motif, it has a significant homology with EPOR, IL-2 receptor beta and gamma, and IL-9 receptor alpha. The murine CRLM-2 gene is composed of 8 exons, and an alternative mRNA splicing generates a variant transcript encoding a soluble CRLM-2. CRLM-2 is preferentially expressed in hematopoietic cells, particularly in hematopoietic progenitors and myeloid cells. Furthermore, CRLM-2 is constitutively associated with JAK2, a well-known tyrosine kinase that transmits signals from cytokine receptors. These data strongly suggest that CRLM-2 is a novel cytokine receptor involved in the regulation of hematopoietic system.
authors.affiliation: Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, and CREST (JST), Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hiroyama
authors.forename: A
authors.initials: A
authors.lastname: Iwama
authors.forename: Y
authors.initials: Y
authors.lastname: Morita
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Shibuya
authors.forename: H
authors.initials: H
authors.lastname: Nakauchi
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006413:Hematopoietic System / Q000276:immunology*; D007136:Immunoglobulins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018121:Receptors, Cytokine / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007136:Immunoglobulins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; C410591:Tslpr protein, mouse