Polymorphisms in FcepsilonRI beta chain do not affect IgE-mediated mast cell activation.
Genetic polyymorphisms that result in three amino acid changes in FcepsilonRI beta chain (Ile(181)-->Leu, Val(183)-->Leu, and Glu(237)-->Gly) have been identified as candidates that associate with allergic disorders such as atopy and asthma. To elucidate the biological significance of these polymorphisms in regulating the expression and function of FcepsilonRI, we generated four types of transfectants that express wild-type or mutant mouse beta chains corresponding to these human variants by retrovirus-mediated gene transfer into beta chain-deficient mouse-derived mast cells. No significant functional differences between the wild-type beta chain transfectant and any of the mutant beta chain transfectants were observed in beta-hexosaminidase release, intracellular calcium mobilization, or cytokine and leukotriene C(4) production in response to FcepsilonRI crosslinking. Our results suggest that these polymorphisms in FcepsilonRI beta chain do not affect FcepsilonRI-mediated mast cell activation at least in our mouse in vitro system.
Furumoto
Y
Y
Allergy Research Center, Department of Immunology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Hiraoka
S
S
Kawamoto
K
K
Masaki
S
S
Kitamura
T
T
Okumura
K
K
Ra
C
C
D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D015550:Cell Degranulation; D016207:Cytokines / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism*; D066298:In Vitro Techniques; D008407:Mast Cells / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016297:Mutagenesis, Site-Directed; D011110:Polymorphism, Genetic; D051381:Rats; D017455:Receptors, IgE / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D016207:Cytokines; D017931:DNA Primers; D017455:Receptors, IgE; D007073:Immunoglobulin E; D001619:beta-N-Acetylhexosaminidases; D002118:Calcium