Expression of measles virus antigens in Streptococcus gordonii.
The measles virus proteins haemagglutinin (HA) and fusion protein (F), which together mediate attachment and penetration of the virus in the host cell and can elicit production of neutralising antibodies in the course of natural infection were expressed in the vaccine vector Streptococcus gordonii, a Gram-positive bacterium normally present in the human oral cavity. HA and F were expressed as fusion proteins attached to the bacterial surface, and were both found to be immunogenic when the recombinant S. gordonii were inoculated subcutaneously in mice.
Maggi
T
T
Dipartimento di Biologia Molecolare, Università di Siena, Italy.
Oggioni
M R
MR
Medaglini
D
D
Bianchi Bandinelli
M L
ML
Soldateschi
D
D
Wiesmüller
K H
KH
Muller
C P
CP
Valensin
P E
PE
Pozzi
G
G
D000818:Animals; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D005260:Female; D006389:Hemagglutinins, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology; D013291:Streptococcus / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D008458:Measles Vaccine; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; C032397:hemagglutinin protein G, measles virus