title: A monoclonal blocking EIA for herpes simplex virus type 2 antibody: validation for seroepidemiological studies in Africa.
abstract: A competitive type-specific enzyme-linked immunosorbent assay (ELISA) for herpes simplex virus type 2 (HSV-2) antibody was developed using an infected cell antigen and a monoclonal antibody to glycoprotein G-2. This assay has been validated for use for epidemiological studies using a large panel of sera collected in rural Uganda and a panel of 143 sera characterised previously by Western blotting, the 'gold standard' for HSV type-specific serology. This evaluation was found to have a sensitivity of 96% and a specificity of 91% in comparison with Western blot on 143 sera from clinic patients. The ELISA had a sensitivity of 93% and a specificity of 91% in comparison with Western blot on 495 sera collected in Uganda. The assay showed good reproducibility and a low percentage of sera gave equivocal results, indicating its suitability for epidemiological studies.
authors.affiliation: Enteric and Respiratory Virus Laboratory Virus, Central Public Health Laboratory, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Gopal
authors.forename: T
authors.initials: T
authors.lastname: Gibbs
authors.forename: M J
authors.initials: MJ
authors.lastname: Slomka
authors.forename: J
authors.initials: J
authors.lastname: Whitworth
authors.forename: L M
authors.initials: LM
authors.lastname: Carpenter
authors.forename: A
authors.initials: A
authors.lastname: Vyse
authors.forename: D W
authors.initials: DW
authors.lastname: Brown
mesh_terms: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006561:Herpes Simplex / Q000453:epidemiology* / Q000821:virology; D018258:Herpesvirus 2, Human / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014454:Uganda / Q000453:epidemiology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral