title: dlarp, a new candidate Hox target in Drosophila whose orthologue in mouse is expressed at sites of epithelium/mesenchymal interactions.
abstract: Hox complex genes are key developmental regulators highly conserved throughout evolution. They encode transcription factors that initiate genetic programs of diversified morphogenesis along the anteroposterior embryonic axis. We report the characterization of the novel Drosophila Hox target gene dlarp, isolated from a further screen of a previously described library of genomic DNA fragments associated in vivo with Ultrabithorax proteins. The dlarp spatio-temporal pattern of transcription in wild-type and homeotic mutant embryos is consistent with a positive regulation by Sex combs reduced and Ultrabithorax in the parasegment 2 ectoderm and the abdominal mesoderm, respectively. The teashirt gene product, thought to act in concert with Hox proteins, is also required for the transcriptional control of this target. Search in databases revealed that dlarp has been highly conserved during evolution. The embryonic expression pattern of the mouse orthologue does not support a function downstream of Hox proteins. It is mainly transcribed in neural structures and in developing organs characterized by epithelial-mesenchymal interactions.
authors.affiliation: Laboratoire de Génétique et Physiologie du Développement, Institut de Biologie du Développement de Marseille, CNRS/Université de la Méditerranée, Parc Scientifique de Luminy, Marseille, France.
authors.forename: S
authors.initials: S
authors.lastname: Chauvet
authors.forename: C
authors.initials: C
authors.lastname: Maurel-Zaffran
authors.forename: R
authors.initials: R
authors.lastname: Miassod
authors.forename: N
authors.initials: N
authors.lastname: Jullien
authors.forename: J
authors.initials: J
authors.lastname: Pradel
authors.forename: D
authors.initials: D
authors.lastname: Aragnol
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000033:anatomy & histology / Q000378:metabolism*; D019143:Evolution, Molecular; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins; D051381:Rats; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D004247:DNA; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000097585:SS-B Antigen; C485865:Larp protein, Drosophila; C412461:Larp1 protein, mouse; C059627:Scr protein, Drosophila; C489361:Tshz1 protein, mouse; C489362:Tshz2 protein, mouse; C066177:Ubx protein, Drosophila; C066830:tsh protein, Drosophila