Sequence annotation of nuclear receptor ligand-binding domains by automated homology modeling.
The quality of three-dimensional homology models derived from protein sequences provides an independent measure of the suitability of a protein sequence for a certain fold. We have used automated homology modeling and model assessment tools to identify putative nuclear hormone receptor ligand-binding domains in the genome of Caenorhabditis elegans. Our results indicate that the availability of multiple crystal structures is crucial to obtaining useful models in this receptor family. The majority of annotated mammalian nuclear hormone receptors could be assigned to a ligand-binding domain fold by using the best model derived from any of four template structures. This strategy also assigned the ligand-binding domain fold to a number of C.elegans. sequences without prior annotation. Interestingly, the retinoic acid receptor crystal structure contributed most to the number of sequences that could be assigned to a ligand-binding domain fold. Several causes for this can be suggested, including the high quality of this protein structure in terms of our assessment tools, similarity between the biological function or ligand of this receptor and the modeled genes and gene duplication in C.elegans.
Françoijs
C J
CJ
Laboratory of Biochemistry, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands.
Klomp
J P
JP
Knegtel
R M
RM
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D017173:Caenorhabditis elegans; D019295:Computational Biology / Q000379:methods; D003198:Computer Simulation; D004331:Drosophila melanogaster; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry* / Q000235:genetics*; D018168:Receptors, Retinoic Acid / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment / Q000379:methods*
D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear; D018168:Receptors, Retinoic Acid