Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos.
Based on the assumption that foreign DNA sequences may have increased chance of integration into the host genome if they are flanked by high copy-numbered genomic sequences such as SINEs (short interspersed elements), we investigated the integration frequency of Lac Z reporter gene flanked by a fused B1/B2 in an in vivo system using pronuclear microinjection technique in the mouse. The SINE-flanked DNA showed a 4-fold increased integration frequency of the reporter gene than the control DNA (63% vs. 16%). Moreover, the level of beta-galactosidase expression, estimated from the X-Gal staining intensity in transgenic embryos, was greatly higher in SINE-carrying DNA. These results suggest that the SINE sequences can serve a very useful tool in improving the efficiency of current transgenic animal technology.
Kang
Y K
YK
Animal Development Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon, South Korea.
Park
J S
JS
Lee
C S
CS
Yeom
Y I
YI
Han
Y M
YM
Chung
A S
AS
Lee
K K
KK
D000818:Animals; D004278:DNA, Superhelical; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D051379:Mice; D008845:Microinjections; D011247:Pregnancy; D020086:Short Interspersed Nucleotide Elements; D019076:Transgenes; D001616:beta-Galactosidase / Q000235:genetics*
D004278:DNA, Superhelical; D001616:beta-Galactosidase