Haemonchus contortus (Nematoda: Trichostrongylidae) is much more sensitive than Caenorhabditis elegans (Nematoda: Rhabditidae) to the ovicidal action of thiabendazole.
When eggs of the trichostrongylid nematode Haemonchus contortus were exposed to thiabendazole, the concentration required to prevent hatching in 90% of the eggs (MIC90) was found to be 0.1 microg/ml (using 1% dimethylsulfoxide [DMSO] as solvent). In contrast, eggs of the free-living rhabditid nematode Caenorhabditis elegans hatched at normal rates at a concentration 200 times higher, i.e., 20 microg/ml, and showed only a partial inhibitory effect at a concentration 1,200 times higher, i.e., 120 microg/ml (in 3% DMSO). Because solubility limitations precluded the testing of higher concentrations of thiabendazole, a more soluble derivative, 5-([1-methylethoxy]carbonylamino)-2-(4-thiazloyl)1H-++ +benzimidazolyliminoacetic acid N,N-diethylethanamine salt, was tested against C. elegans eggs. The MIC90 was found to be 400 microg/ml, and although the derivative was not tested against H. contortus eggs, this finding further suggests that C. elegans eggs have an exceptionally low degree of benzimidazole sensitivity.
Fasiuddin
A
A
Department of Biology and Research Institute for Scientists Emeriti, Drew University, Madison, New Jersey 07940, USA.
Campbell
W C
WC
D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects*; D004121:Dimethyl Sulfoxide; D005243:Feces / Q000469:parasitology; D006189:Haemonchus / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects; D012756:Sheep; D012997:Solvents; D013827:Thiabendazole / Q000494:pharmacology*
D000969:Antinematodal Agents; D012997:Solvents; D013827:Thiabendazole; D004121:Dimethyl Sulfoxide