title: Sequencing and characterization of the cryptic plasmid QpRS from Coxiella burnetii.
abstract: Plasmid QpRS from Coxiella burnetii, isolate Priscilla Q177, phase I, has been completely sequenced. DNA for sequencing was amplified with "extra-long" (XL) PCR. The size of the QpRS plasmid sequence was determined to be 39,280 bp, with a G + C content of 39.3%. Putative proteins associated with replication and recombination were identified. The sequence of QpRS plasmid was analyzed for shared and unique regions among C. burnetii plasmids.
authors.affiliation: Justus Liebig University, Institute for Hygiene and Infectious Diseases of Animals, Giessen, Germany. Silke.Lautenschlaeger@vetmed.uni-giessen.de
authors.forename: S
authors.initials: S
authors.lastname: Lautenschläger
authors.forename: H
authors.initials: H
authors.lastname: Willems
authors.forename: C
authors.initials: C
authors.lastname: Jäger
authors.forename: G
authors.initials: G
authors.lastname: Baljer
mesh_terms: D001483:Base Sequence; D016997:Coxiella burnetii / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000032:analysis*; D014176:Protein Biosynthesis; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004269:DNA, Bacterial