title: Determination of hydroperoxides in liposomes by the modified IDF and the modified tiron methods.
abstract: Two new methods, the modified International Dairy Federation and the modified Tiron methods, were developed for the determination of hydroperoxides in liposomes. Interferences of alpha-tocoferol and egg-phosphatidylcholine (EPC) required a solid-phase extraction before the analysis to eliminate alpha-tocoferol and a fluid-fluid extraction based on the solvent triangle of Bligh and Dyer to separate EPC. The developed color, using thiocyanate and Tiron, respectively, as complex-formers for the generated ferri-ions, was measured spectrophotometrically. Both techniques showed good reproducibility and high sensitivity. Peroxide contents of 0.04 and 0.07% (g/g) in EPC samples were easily determined.
authors.affiliation: VUB Pharmaceutical Technology and Physical Pharmacy, Brussels, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Gabriëls
authors.forename: J
authors.initials: J
authors.lastname: Cirunay
authors.forename: M
authors.initials: M
authors.lastname: Alafandy
authors.forename: M
authors.initials: M
authors.lastname: Brisaert
authors.forename: M
authors.initials: M
authors.lastname: de Taevernier
authors.forename: F
authors.initials: F
authors.lastname: Camu
authors.forename: J
authors.initials: J
authors.lastname: Plaizier-Vercammen
mesh_terms: D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D001555:Benzene Derivatives / Q000032:analysis; D006861:Hydrogen Peroxide / Q000032:analysis; D007202:Indicators and Reagents; D008081:Liposomes / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry; D010545:Peroxides / Q000032:analysis*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012996:Solutions; D014810:Vitamin E
chemical_list: D001555:Benzene Derivatives; D007202:Indicators and Reagents; D008081:Liposomes; D010545:Peroxides; D012996:Solutions; D014810:Vitamin E; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D006861:Hydrogen Peroxide; C007164:cumene hydroperoxide