New fluorogenic substrate for the first continuous steroid sulfatase assay.
The screening for new inhibitors of steroid sulfatase requires an efficient test system. To overcome the shortcomings of the available discontinuous fluorimetric assay, several coumarin-type compounds were investigated as potential new substrates. 3,4-Benzocoumarin 7-O-sulfate was found to have appropriate substrate properties for the establishment of the first direct continuous assay of steroid sulfatase.
Bilban
M
M
NOVARTIS Research Institute, Vienna, Austria.
Billich
A
A
Auer
M
M
Nussbaumer
P
P
D001192:Arylsulfatases / Q000037:antagonists & inhibitors*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003374:Coumarins / Q000138:chemical synthesis* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology*; D005470:Fluorometry; D006801:Humans; D007700:Kinetics; D011994:Recombinant Proteins; D043266:Steryl-Sulfatase; D013329:Structure-Activity Relationship; D013429:Sulfatases / Q000037:antagonists & inhibitors
C414669:3,4-benzocoumarin 7-O-sulfate; D003374:Coumarins; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D013429:Sulfatases; C020121:estrone sulfatase; D001192:Arylsulfatases; D043266:Steryl-Sulfatase