Chloramphenicol induces apoptosis in the developing brain.
Programmed cell death was studied in the superior colliculus of the developing rat brain following injections of chloramphenicol. Neonatal rats were either subject to unilateral eye removal or left untouched. Following a 3-h post-operative survival, the animals were perfused with fixatives and frozen sections of their brains were examined for apoptosis after either neutral-red staining, in situ nick-end labeling of fragmented DNA, or immunocytochemistry to activated caspase-3. Chloramphenicol induced apoptosis in control brains and potentiated cell death in deafferented superior colliculi. The results show that CMP has a general pro-apoptotic effect in the developing brain.
Guimarães
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Instituto de Biofísica da UFRJ, Centro de Ciências da Saúde, bloco G, Cidade Universitária, 21949-900, Rio de Janeiro, Brazil. cinthya@biof.ufrj.br
Linden
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D000344:Afferent Pathways / Q000503:physiopathology / Q000601:surgery; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D002701:Chloramphenicol / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003714:Denervation; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011506:Proteins / Q000187:drug effects; D051381:Rats; D013477:Superior Colliculi / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
D011500:Protein Synthesis Inhibitors; D011506:Proteins; D002701:Chloramphenicol; C505410:Casp3 protein, rat; D053148:Caspase 3; D020169:Caspases