Enterococcus faecalis V583 contains a cytochrome bd-type respiratory oxidase.
We have cloned an Enterococcus faecalis gene cluster, cydABCD, which when expressed in Bacillus subtilis results in a functional cytochrome bd terminal oxidase. Our results indicate that E. faecalis V583 cells have the capacity of aerobic respiration when grown in the presence of heme.
Winstedt
L
L
Department of Microbiology, Lund University, Lund, Sweden. lena.winstedt@mikrbiol.lu.se
Frankenberg
L
L
Hederstedt
L
L
von Wachenfeldt
C
C
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism; D003573:Cytochrome b Group; D003580:Cytochromes / Q000235:genetics*; D045222:Electron Transport Chain Complex Proteins; D013293:Enterococcus faecalis / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005810:Multigene Family; D016296:Mutagenesis; D010088:Oxidoreductases / Q000235:genetics*
D003573:Cytochrome b Group; D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D010088:Oxidoreductases; C040077:cytochrome bd terminal oxidase complex, E coli