Do structural deviations between toxins adopting the same fold reflect functional differences?
Three-finger proteins form a structurally related family of compounds that exhibit a great variety of biological properties. To address the question of the prediction of functional areas on their surfaces, we tentatively conferred the acetylcholinesterase inhibitory activity of fasciculins on a short-chain curaremimetic toxin. For this purpose, we assimilated the three-dimensional structure of fasciculin 2 with the one of toxin alpha. This comparison revealed that the tips of the first and second loops, together with the C terminus residue, deviated most. A first recombinant fasciculin/toxin alpha chimera was designed by transferring loop 1 in its entirety together with the tip of loop 2 of fasciculin 2 into the toxin alpha scaffold. A second chimera (rChII) was obtained by adding the point Asn-61 --> Tyr substitution. Comparison of functional and structural properties of both chimeras show that rChII can accommodate the imposed modifications and displays nearly all the acetylcholinesterase-blocking activities of fasciculins. The three-dimensional structure of rChII demonstrates that rChII adopts a typical three-fingered fold with structural features of both parent toxins. Taken together, these results emphasize the great structural flexibility and functional adaptability of that fold and confirm that structural deviations between fasciculins and short-chain neurotoxins do indeed reflect functional diversity.
Ricciardi
A
A
Instituto de Investigaciones Biologicas, Clemente Estable, Montevideo, Uruguay 11600, France.
le Du
M H
MH
Khayati
M
M
Dajas
F
F
Boulain
J C
JC
Menez
A
A
Ducancel
F
F
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002800:Cholinesterase Inhibitors / Q000737:chemistry*; D004546:Elapid Venoms / Q000737:chemistry* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D008958:Models, Molecular; D008969:Molecular Sequence Data; D003473:Neuromuscular Nondepolarizing Agents / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship; D014118:Toxins, Biological / Q000737:chemistry* / Q000235:genetics
D002800:Cholinesterase Inhibitors; D004546:Elapid Venoms; D003473:Neuromuscular Nondepolarizing Agents; D011993:Recombinant Fusion Proteins; D014118:Toxins, Biological; C044937:fasciculin