The role of phosphatidylcholine in fatty acid exchange and desaturation in Brassica napus L. leaves.
The role of phosphatidylcholine (PC) in fatty acid exchange and desaturation was examined and compared with that of monogalactosyldiacylglycerol (MGDG) in Brassica napus leaves using (14)C-labelling in vivo. Data are presented which indicate that in the chloroplast newly formed saturated (palmitic acid, 16:0) and monounsaturated (oleic acid, 18:1) fatty acid is incorporated into MGDG and desaturated in situ. In the non-plastidic compartments, however, newly formed fatty acid is exchanged with polyunsaturated fatty acid in PC, the probable major site of subsequent desaturation. The unsaturated fatty acid is released to the acyl-CoA pool, which is then used to synthesize diacylglycerol (DAG) containing a high level of unsaturated fatty acid. This highly unsaturated DAG may be the source for the biosynthesis of other cellular glycerolipids. The generally accepted pathway in which PC is synthesized from molecular species of DAG containing 16:0 and 18:1 followed by desaturation of the 18:1 to linoleic (18:2) and linolenic (18:3) acids is questioned.
Williams
J P
JP
Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario, Canada M5S 3B2. williams@botany.utoronto.ca
Imperial
V
V
Khan
M U
MU
Hodson
J N
JN
D001937:Brassica / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D019787:Linoleic Acid / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry*; D008954:Models, Biological; D019301:Oleic Acid / Q000378:metabolism; D010713:Phosphatidylcholines / Q000096:biosynthesis* / Q000737:chemistry / Q000502:physiology*; D013997:Time Factors
D005227:Fatty Acids; D008055:Lipids; D010713:Phosphatidylcholines; D019301:Oleic Acid; D019787:Linoleic Acid