title: Cloning and sequence of the gene encoding a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-9) from Escherichia coli in Spain.
abstract: A new CTX-M-type beta-lactamase (CTX-M-9) has been cloned from a clinical cefotaxime-resistant Escherichia coli strain. Despite the close identity that exists between the CTX-M-9 and Toho-2 beta-lactamases (88%), the 35 amino acids located between residues Ala-185 and Ala-219 are totally different in both enzymes. Outside of this region there are only six amino acids substitutions between both proteins.
authors.affiliation: Departament de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma 08025 Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Sabaté
authors.forename: R
authors.initials: R
authors.lastname: Tarragó
authors.forename: F
authors.initials: F
authors.lastname: Navarro
authors.forename: E
authors.initials: E
authors.lastname: Miró
authors.forename: C
authors.initials: C
authors.lastname: Vergés
authors.forename: J
authors.initials: J
authors.lastname: Barbé
authors.forename: G
authors.initials: G
authors.lastname: Prats
mesh_terms: D000595:Amino Acid Sequence; D002439:Cefotaxime / Q000378:metabolism / Q000494:pharmacology; D002511:Cephalosporins / Q000378:metabolism / Q000494:pharmacology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D006868:Hydrolysis; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013030:Spain; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D002511:Cephalosporins; D029968:Escherichia coli Proteins; C412613:CTX-M-9 protein, E coli; D001618:beta-Lactamases; D002439:Cefotaxime