[Differences in phorbol-dependent phosphorylation of regulatory proteins and contraction of phasic and tonic smooth muscle].
Smooth muscles are divided into slowly contracting tonic and relatively fast phasic muscles. In both cases Ca2+ is a key mediator of the contractile response. However, the appearance of a tonic component during sphincter or arterial muscle contraction and its absence in contracting visceral smooth muscle is characteristic of their difference. We have found that in chicken tissues phorbol 12,13-dibutyrate (PDBu) induces a sustained contraction in carotid arterial muscle, but provokes no contraction in phasic gizzard smooth muscle. Next we were aimed to find differences in PDBu-induced phosphorylation of the key proteins involved in regulation of smooth muscle contraction, i.e. caldesmon, myosin light chain kinase (MLCK), and the myosin light chain kinase-related protein (KRP, also known as telokin). Two correlative differences were observed. 1. PDBu stimulated phosphorylation of MLCK in tonic smooth muscle and had no effect on the level of MLCK phosphorylation in phasic muscle. Phosphopeptide mapping suggests the involvement of mitogen-activated protein (MAP) kinases in phosphorylation of MLCK in situ. 2. PDBu induced phosphorylation of MAP-kinase sites in caldesmon in both types of smooth muscle, but this phosphorylation had no significant effect on caldesmon functional activity in vitro. For the first time we have shown that in gizzard PDBu also stimulates a yet unknown transitory caldesmon-kinase different from protein kinase, C, Ca2+/calmodulin-dependent kinase II and casein kinase CK2. 3. No significant difference was found in the kinetics of PDBu-dependent phosphorylation of KRP in tonic and phasic smooth muscles. KRP was also demonstrated to be a major phosphoprotein in smooth muscle phosphorylated in vivo at several sites located within its N-terminal sequence. Protein kinases able to phosphorylate these sites were identified in vitro. Among them, MAP-kinase was suggested to phosphorylate a serine residue homologous to that phosphorylated in MLCK. 4. p42erk2 and p38 MAP-kinases were found in phasic and tonic smooth muscles. Both were responsive to PDBu in cultured chicken aortic smooth muscle cells, and their role in phosphorylation of MLCK and low molecular weight isoform of caldesmon was evaluated.
Vorotnikov
A V
AV
Institute of Experimental Cardiology, Russian Cardiology Research Centre, Ministry of Public Health, Moscow.
Krymskiĭ
M A
MA
Chibalina
M V
MV
Kudriashov
D S
DS
Shirinskiĭ
V P
VP
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D002645:Chickens; D066298:In Vitro Techniques; D016547:Kinesins; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D010449:Peptide Mapping; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology*; D010766:Phosphorylation; D051381:Rats
D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D009124:Muscle Proteins; C074152:kinesin-II; D015240:Phorbol 12,13-Dibutyrate; D009219:Myosin-Light-Chain Kinase; D020928:Mitogen-Activated Protein Kinases; D016547:Kinesins
Razlichia v forbolzavisimom fosforilirovanii regliatornykh belkov i sokrashchenii faznykh i tonicheskikh gladkikh myshts.