High-performance liquid chromatography of monoamines on phenyl-bound sorbents using organic free mobile phases.
Tryptophan and its metabolites, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindolacetic acid, as well as dopamine, homovanilic acid and 2,3-dihydroxyphenylacetic acid, were separated on phenyl bound silica gel using isocratic elution with phosphate buffer. The method was successfully transferred to several other phenyl HPLC columns from different manufacturers simply by adjusting the pH of the buffer. The method has been validated by the determination of the level of monoamines in rat hypothalamus.
Mezine
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Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
Chavez
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M
Vatamaniuk
M
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Teff
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K
D015102:3,4-Dihydroxyphenylacetic Acid / Q000302:isolation & purification; D006916:5-Hydroxytryptophan / Q000302:isolation & purification; D000818:Animals; D015306:Biogenic Monoamines / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000302:isolation & purification; D006719:Homovanillic Acid / Q000302:isolation & purification; D006897:Hydroxyindoleacetic Acid / Q000302:isolation & purification; D007031:Hypothalamus / Q000737:chemistry; D051381:Rats; D012701:Serotonin / Q000302:isolation & purification; D014364:Tryptophan / Q000302:isolation & purification
D015306:Biogenic Monoamines; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D006916:5-Hydroxytryptophan; D004298:Dopamine; D006719:Homovanillic Acid