Construction of a physical and transcript map flanking the imprinted MEST/PEG1 region at 7q32.
MEST/PEG1, a gene expressed paternally in mesodermal derivatives in early embryonic stages, is the first imprinted gene mapped to chromosome 7q32. Since imprinted genes are clustered in general at a chromosomal region, we speculated that a similar imprinted-gene cluster may exist at chromosome region 7q32 and that the functions of some such genes may contribute to the phenotype of Silver-Russell syndrome including maternal uniparental disomy for chromosome 7 (maternal UPD7). As an initial step toward the isolation of imprinted genes at 7q32, we adopted an integrated approach involving the construction of a PAC contig and ESTs mapping in the vicinity of MEST. Here, we have constructed a complete contig of PAC and BAC clones and a transcript map spanning the entire approximately 1-Mb region between D7S530 and D7S649. We developed 60 novel STSs and precisely mapped 47 genes/ESTs. This map contains a putative autistic disorder locus that has been suggested to be localized near markers D7S530 and D7S684. This integrated physical and transcript map provides a valuable resource for identification of an imprinted gene(s) in this region as well as a candidate gene(s) for autistic disorder.
Hayashida
S
S
Department of Human Genetics, Nagasaki University School of Medicine, Sakamoto 1-12-4, Nagasaki, 852-8523, Japan.
Yamasaki
K
K
Asada
Y
Y
Soeda
E
E
Niikawa
N
N
Kishino
T
T
D001483:Base Sequence; D002897:Chromosomes, Human, Pair 7; D017931:DNA Primers; D018392:Genomic Imprinting; D006801:Humans; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D017931:DNA Primers; D011506:Proteins; D012333:RNA, Messenger; C103637:mesoderm specific transcript protein