Sialyl Lewis(x) (CD15s) monitoring as a means to select antirejection therapy in patients with rejection after renal transplantation: CD15s monitoring for treatment and diagnosis in patients with acute rejection after renal transplantation.
BACKGROUND
Sialyl Lewis(x) (CD15s), which is known to serve as a ligand for the cell adhesion molecules E-selectin and P-selectin, is expressed on peripheral lymphocytes in renal transplant patients with rejection. In this study, we examined whether CD15s monitoring could differentiate the patients with rejection in whom steroids were effective from those in whom steroids were not effective.


METHODS
To investigate CD15s expression on peripheral lymphocytes, flow cytometry was performed before and after steroid pulse therapy in 20 recipients with rejection after renal transplantation, including 5 recipients resistant to steroid therapy. We also compared CD15s expression with pathological findings before and after steroid pulse therapy.


RESULTS
CD15s expression was stronger before steroid pulse therapy in the 5 patients resistant to steroids than in the 15 patients responsive to steroid treatment. In addition, CD15s expression remained high without any pathological improvement in the 5 patients resistant to steroids after steroid treatment, although CD15s recovered to normal levels with remarkable improvement of pathological findings in the other 15 patients. Five patients in whom steroids were not effective, had full recovery of serum creatinine levels as well as CD15s expression after muromonab (OKT3) therapy.


CONCLUSIONS
CD15s monitoring might help clinicians to select antirejection therapy.
Ishida
H
H
Department of Surgery III, Tokyo Women's Medical University, Japan.
Koyama
I
I
Sawada
T
T
Murakami
T
T
Tojimbara
T
T
Nakajima
I
I
Tanabe
K
K
Yamaguchi
Y
Y
Fuchinoue
S
S
Toma
H
H
Agishi
T
T
D000328:Adult; D015415:Biomarkers / Q000097:blood*; D003404:Creatinine / Q000097:blood; D016903:Drug Monitoring / Q000379:methods; D004351:Drug Resistance; D005260:Female; D005434:Flow Cytometry; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis* / Q000188:drug therapy*; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D016030:Kidney Transplantation / Q000276:immunology*; D016256:Lewis X Antigen / Q000097:blood*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D016853:Muromonab-CD3 / Q000627:therapeutic use*; D009844:Oligosaccharides / Q000097:blood*; D000080506:Sialyl Lewis X Antigen; D013256:Steroids / Q000627:therapeutic use*
D015415:Biomarkers; D007166:Immunosuppressive Agents; D016256:Lewis X Antigen; D016853:Muromonab-CD3; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D013256:Steroids; D003404:Creatinine