title: Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli.
abstract: Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.
authors.affiliation: Abteilung Allgemeine Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg-August-Universität, 37077 Göttingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Henne
authors.forename: R A
authors.initials: RA
authors.lastname: Schmitz
authors.forename: M
authors.initials: M
authors.lastname: Bömeke
authors.forename: G
authors.initials: G
authors.lastname: Gottschalk
authors.forename: R
authors.initials: R
authors.lastname: Daniel
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D004950:Esterases / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012988:Soil Microbiology; D014280:Triglycerides / Q000378:metabolism; D014304:Triolein / Q000378:metabolism
chemical_list: D014280:Triglycerides; D014304:Triolein; D004950:Esterases; D008049:Lipase; C005830:tributyrin