[Diagnostic value of some cytokines levels in chronic myelogenous leukemia].
Some cytokines play an important role in aetiology and pathogenesis of leukaemia. Majority of data available in the literature relates to a single cytokine in the cell culture in vitro. In patients with chronic myelogenous leukaemia several different cytokines are probably active. Therefore, this study aimed to determine the concentrations of interleukin-1 beta, G-CSF, L-selectin in leukaemia cell culture supernatant, broken granulocytes and plasma in the course of disease exacerbation and remission. Cytokines have been assayed with available immunoenzymatic kits of ELISA type. IL-1 beta, G-CSF and L-selectin levels have been increased in cell supernatant but IL-1 beta level has been decreased in non-stimulated broken granulocytes. G-CSF levels have been low both in stimulated and non-stimulated granulocytes. In comparison to control the lowered levels of G-CSF and L-selectin have been observed in blood plasma of patients with CML. Different levels of assayed cytokines and adhesion molecule may suggest their contribution to leukaemia cells proliferation regulation.
Kiersnowska-Rogowska
B
B
Kliniki Hematologii Akademii Medycznej w Białymstoku.
Parfieńczyk
A
A
Rogowski
F
F
Citko
A
A
Jaroszewicz
E
E
D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000276:immunology*; D012074:Remission Induction
D016207:Cytokines
Wartość diagnostyczna aktywności stezeń cytokin w przewlekłej białaczce szpikowej.