title: All major regions of FtsK are required for resolution of chromosome dimers.
abstract: Resolution of chromosome dimers, by site-specific recombination between dif sites, is carried out in Escherichia coli by XerCD recombinase in association with the FtsK protein. We show here that a variety of altered FtsK polypeptides, consisting of the N-terminal (cell division) domain alone or with deletions in the proline-glutamine-rich part of the protein, or polypeptides consisting of the C-terminal domain alone are all unable to carry out dif recombination. Alteration of the putative nucleotide-binding site also abolishes the ability of FtsK to carry out recombination between dif sites.
authors.affiliation: Institute of Cell & Molecular Biology, University of Edinburgh, Scotland. Dboyle@skcc.org
authors.forename: D S
authors.initials: DS
authors.lastname: Boyle
authors.forename: D
authors.initials: D
authors.lastname: Grant
authors.forename: G C
authors.initials: GC
authors.lastname: Draper
authors.forename: W D
authors.initials: WD
authors.lastname: Donachie
mesh_terms: D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000378:metabolism*; D011995:Recombination, Genetic; D017384:Sequence Deletion
chemical_list: D029968:Escherichia coli Proteins; C096452:FtsK protein, E coli; D008565:Membrane Proteins