[Activity of N-b-D-acetylglucosaminidase molecular forms in amniotic fluid of tobacco smoking pregnant women with oligohydramnios or premature rupture of the membrane complications].
OBJECTIVE
Morphological lesions in placenta and changed metal distribution through placenta, which were observed in pregnancy exposed to tobacco smoke, could cause alternations in lysosomal enzymes secretion to amniotic fluid.


METHODS
Assessment of total activity and molecular forms of N-beta-D-acetylglucosaminidase in amniotic fluid in pregnancy with oligohydramnios or PROM.


METHODS
The materials for studies were amniotic fluids collected from pregnancy with oligohydramnios and from pregnancy with PROM. Determination of N-beta-D-acetylglucosaminidase activity was performed with use of sodium salt of 3-crezolosulfthaleinyl-N-beta-D-acetylglucosamide as a substrate (test Boehringer, Niemcy). Creatinine was determined by kinetic method (test of Analco-GBG firm). Protein was determine by Bredford method. NAG-B was assessed after previous thermal NAG-A inactivation in 56 degrees C, for 2 hours and the difference between activity of total form and termostabile form of NAG were assessed. The microsomal and cytosol fractions were separated by ultracentrifugation.


CONCLUSIONS
The statistically significant growth of NAG activity in amniotic fluid in pregnant with oligohydramnios was observed. The cytosol form took over 90% of whole activity. The activity of NAG microsomal fraction was lower in amniotic fluid in women with PROM diagnosis. The positive coleration between NAG-A activity and the Pb level was observed in this group of women as well. In the second group of women with oligohydramnios, the positive coleration between NAG and Cd level, NAG-A and level, NAG-B and Pb level were demonstrated. Obtained results show on damage of placental and foetal membranes cell structure. It could be caused by metal ions cumulation and releasing of molecular N-beta-D-acetylglucosaminase form to amniotic fluid.
Milnerowicz
H
H
Pracowni Badań nad Metalotioneina Katedry i Zakładu Toksykologii AM we Wrocławiu.
Zalewski
J
J
Milnerowicz-Nabzdyk
E
E
Woytoń
J
J
D000118:Acetylglucosaminidase / Q000032:analysis* / Q000378:metabolism*; D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000175:diagnosis* / Q000201:enzymology*; D006801:Humans; D008875:Middle Aged; D016104:Oligohydramnios / Q000175:diagnosis* / Q000201:enzymology*; D011247:Pregnancy; D011248:Pregnancy Complications; D012907:Smoking / Q000009:adverse effects*
D000118:Acetylglucosaminidase
Aktywność molekularnych form N-acetylo-beta-D-glukozaminidazy w płynie owodniowym kobiet palacych papierosy podczas ciazy powikłanej małowodziem lub przedwczesnym odpływaniem płynu owodniowego.