title: The mouse ZFH-4 protein contains four homeodomains and twenty-two zinc fingers.
abstract: We isolated the mouse zfh-4 cDNA which is 12 kb long and capable of encoding a 3,550-amino acid protein containing four homeodomains and 22 zinc fingers including two pseudo zinc finger motifs. The mouse ZFH-4 is 51% homologous to the mouse ATBF1 and 23% to the Drosophila ZFH-2. The homeodomain and zinc finger regions are highly conserved between ZFH-4 and ATBF1 except that one zinc finger is missing in ZFH-4. Analysis of partial genomic sequences showed that the mouse zfh-4 and ATBF1 genes are similar in exon-intron organization. RT-PCR analysis of zfh-4 transcripts in adult mouse tissues showed that zfh-4 expression was low but reproducibly detectable in brain, heart, lung and muscle. In these mouse tissues, ATBF1 transcripts were poorly amplified by PCR under the conditions where zfh-4 transcripts were amplified, suggesting that the expression of zfh-4 mRNA is higher than that of ATBF1 mRNA. Other comparative analysis suggests functional similarities and dissimilarities between ZFH-4 and ATBF1.
authors.affiliation: Department of Biochemistry, Showa Pharmaceutical University, Machida, Tokyo, 194-8543, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sakata
authors.forename: K
authors.initials: K
authors.lastname: Hemmi
authors.forename: M
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Noguchi
authors.forename: D
authors.initials: D
authors.lastname: Ma
authors.forename: M
authors.initials: M
authors.lastname: Sasahara
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Hori
authors.forename: T
authors.initials: T
authors.lastname: Tamaoki
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics*; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018398:Homeodomain Proteins; D012333:RNA, Messenger; C489511:Zfhx3 protein, mouse; C093452:Zfhx4 protein, mouse