title: Identification of PLC beta and PKC in pheromone receptor neurons of Antheraea polyphemus.
abstract: Two proteins of the IP3 transduction pathway were identified by Western blots in homogenates of isolated pheromone-sensitive sensilla of the silkmoth Antheraea polyphemus. A 110 kDa protein was recognized by an antiserum raised against the Drosophila phospholipase C beta (PLC beta p121) and a 80kDa protein was labelled by an antiserum against a synthetic peptide of a conserved region of protein kinase C (PKC). Incubation of homogenized sensory hairs with the main sex pheromone component, (E,Z) 6-11 hexadecadienyl acetate, resulted in a 6-fold increase in the activity of PKC compared to controls without pheromone. In contrast, incubation with pheromone did not affect the activity of protein kinase A (PKA). Activation of PKC by the membrane permeable dioctanoylglycerol led to excitation of the pheromone-sensitive receptor neurons. These data support the current concept that pheromone perception of moths is mediated by the IP3 transduction pathway.
authors.affiliation: Max-Planck-Institut für Verhaltensphysiologie, Seewiesen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Maida
authors.forename: A
authors.initials: A
authors.lastname: Redkozubov
authors.forename: G
authors.initials: G
authors.lastname: Ziegelberger
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000818:Animals; D002628:Chemoreceptor Cells / Q000378:metabolism*; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000502:physiology; D007527:Isoenzymes / Q000378:metabolism*; D009036:Moths / Q000378:metabolism*; D009475:Neurons, Afferent / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010675:Pheromones / Q000494:pharmacology; D054799:Phospholipase C beta; D011493:Protein Kinase C / Q000378:metabolism*; D012016:Reference Values; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: C413925:6,11-hexadecadienyl acetate; D000085:Acetates; D004075:Diglycerides; D007527:Isoenzymes; D010675:Pheromones; C044295:1,2-dioctanoylglycerol; D011493:Protein Kinase C; D010738:Type C Phospholipases; D054799:Phospholipase C beta