title: Lack of GATC sites in the genome of Streptococcus bovis bacteriophage F4.
abstract: A strong bias against GATC sites was observed in the genome of phage F4, a lytic Streptococcus bovis bacteriophage. Only three GATC sites were found within the 60.4-kbp genome of this phage. The comparative lack of GATC sequences within the F4 genome was probably not due to dam methylation, as no modification within this site was detected using methylation-sensitive isoschizomer pair restriction endonuclease analysis. The short oligonucleotide composition of available S. bovis DNA sequences suggested the existence of an unknown mechanism for counterselection of GATC sites in S. bovis bacteriophages.
authors.affiliation: Institute of Animal Physiology of the Slovak Academy of Sciences, Kosice. styriak@mail2.saske.sk
authors.forename: I
authors.initials: I
authors.lastname: Styriak
authors.forename: P
authors.initials: P
authors.lastname: Pristas
authors.forename: P
authors.initials: P
authors.lastname: Javorský
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D001665:Binding Sites; D015280:DNA Restriction-Modification Enzymes; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D016679:Genome, Viral; D012418:Ruminants / Q000382:microbiology*; D016985:Streptococcus bovis / Q000821:virology*
chemical_list: D015280:DNA Restriction-Modification Enzymes; D004279:DNA, Viral