title: Usefulness of a toothbrush as a source of evidential DNA for typing.
abstract: We investigated the usefulness of a toothbrush as a source of DNA for an unidentified cadaver. Ten toothbrushes were obtained from ten individuals along with their peripheral blood. We recovered from 10 to 430 ng of DNA from all but one of the toothbrushes. All ten toothbrushes, including the one containing no detectable DNA by fluorometry, were typed correctly at all of the loci tested, including nine STRs. Three toothbrushes obtained in two actual deaths also identified two victims and one suspect. Therefore, toothbrushes seem to be useful as a source of evidential DNA for personal identification.
authors.affiliation: Department of Legal Medicine and Bioethics, Postgraduate School of Medicine, Nagoya University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Yoshimoto
authors.forename: H
authors.initials: H
authors.lastname: Nozawa
authors.forename: H
authors.initials: H
authors.lastname: Ohtaki
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Tamaki
authors.forename: Y
authors.initials: Y
authors.lastname: Katsumata
mesh_terms: D002102:Cadaver; D004247:DNA / Q000302:isolation & purification*; D005470:Fluorometry; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D013048:Specimen Handling; D020080:Tandem Repeat Sequences / Q000235:genetics*; D014099:Toothbrushing
chemical_list: D004247:DNA