title: Unaltered distribution of laminins, fibronectin, and tenascin in celiac intestinal mucosa.
abstract: Extensive remodeling of the extracellular matrix (ECM) occurs in inflammatory tissues. The celiac lesion in the small intestine is characterized by inflammation accompanied by profound morphological alterations. We used immunohistochemistry to determine the distribution of laminin, fibronectin, and tenascin isoforms in small intestinal biopsies of untreated patients with celiac disease. In normal mucosa, the distribution of laminin isoforms defines three epithelial basement membrane (BM) zones. We found that the organization of these zones was maintained in the celiac mucosa. Thus, components of laminin-5 (alpha3 and beta3) were found in the surface epithelial BM, laminin alpha2 chain was found selectively at crypt bottoms, and laminin alpha5 chain was the sole alpha-type chain in middle crypt BMs. Likewise, the distribution of fibronectin and tenascin resembled that of the normal gut. The organization of pericryptal fibroblasts and lamina propria smooth muscle strands, as defined by immunostaining for alpha-smooth muscle actin, also remained unchanged in the celiac mucosa. Unexpectedly, major ECM changes were not detected in the celiac lesion.
authors.affiliation: Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland. matti.korhonen@helsinki.fi
authors.forename: M
authors.initials: M
authors.lastname: Korhonen
authors.forename: M
authors.initials: M
authors.lastname: Ormio
authors.forename: R E
authors.initials: RE
authors.lastname: Burgeson
authors.forename: I
authors.initials: I
authors.lastname: Virtanen
authors.forename: E
authors.initials: E
authors.lastname: Savilahti
mesh_terms: D000293:Adolescent; D000328:Adult; D001485:Basement Membrane / Q000378:metabolism; D002446:Celiac Disease / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000098:blood supply / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009130:Muscle, Smooth / Q000378:metabolism; D019063:Tenascin / Q000378:metabolism*
chemical_list: D005353:Fibronectins; D007797:Laminin; D019063:Tenascin