Microheterogeneity of tubulin proteins in neuronal and glial cells from the mouse brain in culture.
The microheterogeneity of the alpha and beta isoforms of tubulin in brain cells in culture was studied. The cells were prepared from two precise regions of the embryonic mouse brain (ED15), the striatum and the mesencephalon. It was possible to maintain virtually pure cultures of neuronal or glial cells up to 1 and 4 weeks in vitro, respectively. The tubulin heterogeneity of striatal and mesencephalic neurons was found to be very similar after a few days in culture. More precise examination of pure neurons from the striatum revealed that their tubulin content after 7 days in vitro exhibited the same degree of complexity as a control extract from a 4 day-old mouse brain. In fact, we could detect the presence of at least six alpha and nine beta tubulin isoforms. Among these isoforms a specific family of beta proteins (beta' tubulin) and the more acidic alpha proteins were present. Since these isoforms have, up to now, been found only in tubulin extracts prepared from the nervous system, our experiments suggest that they belong to the neuronal subpopulation of this tissue. This point is reinforced by their complete absence from the tubulin proteins extracted from pure glial cells even after several weeks in vitro. These results lead us to propose that brain tubulin microheterogeneity is associated with the presence of neurons and not of glia and may, therefore, play a specific role in maintaining neuronal shape and function.
Moura Neto
V
V
Laboratoire de Biochimie Cellulaire, INSERM U.114, Collège de France, Paris, France.
Mallat
M
M
Jeantet
C
C
Prochiantz
A
A
D000818:Animals; D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D051379:Mice; D009457:Neuroglia / Q000737:chemistry* / Q000166:cytology; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D020033:Protein Isoforms / Q000032:analysis; D014404:Tubulin / Q000032:analysis*
D020033:Protein Isoforms; D014404:Tubulin