Detection of human serum antibodies against type-specifically reactive peptides from the N-terminus of glycoprotein B of herpes simplex virus type 1 and type 2 by surface plasmon resonance.
A single-step surface plasmon resonance protocol for the detection of antibodies against herpes simplex virus type 1 and type 2 (HSV-1, HSV-2) in human sera was established using the BIAcore system. Two peptides from corresponding segments of the N-terminus of HSV-1 and HSV-2 glycoprotein B (gB), i.e. peptide gB-1 (60-73) (GAAPTGDPKPKKNK) and peptide gB-2 (55-68) (SPATTKARKRKTKK), were identified as immunogenic. Employing both peptides as diagnostic antigens in the surface plasmon resonance assay, a sensitivity for the detection of HSV-1 and HSV-2 type-specific antibodies of 83 and 86%, respectively, was achieved as compared with immunoblotting as a reference method. Peptide gB-1 (60-73) allowed the discrimination between HSV-1 and HSV-2 type-specific antibodies with a specificity of 67%, whereas peptide gB-2 (55-68) reacted in a strictly HSV-2 type-specific manner. It is concluded that peptides from the N-terminus of gB-1 and gB-2 are recognized predominantly by human sera in an HSV-specific manner. Peptide gB-2 (55-68) can be employed successfully for the determination of type-specific antibodies against HSV-2.
Wittekindt
C
C
Klinik für Hals-, Nasen-, Ohrenkrankheiten, Universität Köln, Germany.
Fleckenstein
B
B
Wiesmüller
K
K
Eing
B R
BR
Kühn
J E
JE
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood* / Q000737:chemistry; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000276:immunology; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D016415:Sequence Alignment; D020349:Surface Plasmon Resonance / Q000379:methods*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D010446:Peptide Fragments