[Effect of subinhibitory concentrations of various antimicrobials on insoluble glucan production, polystyrene adherence and bacterial surface hydrophobicity of Streptococcus sobrinus].
The effect of subinhibitory concentrations (1/2, 1/4, 1/8 of the MIC) of amoxicillin, teicoplanin, clindamycin and erythromycin on the factors influencing the adherence and pathogenicity of Streptococcus sobrinus was evaluated. These factors included insoluble glucan, polystyrene adherence and bacterial surface hydrophobicity. Insoluble glucan was detected using a spectrophotometric method; polystyrene adherence was assessed using microtiter plates; and surface hydrophobicity was determined using a biphasic system (water/p-xiline). Amoxicillin and teicoplanin induced a statistically significant decrease in adherence at subinhibitory concentrations, and they also decreased the hydrophobicity. However, clindamycin and erythromycin were unable to decrease these parameters. The adherence and hydrophobicity of S. sobrinus was also dependent on the concentration of sucrose in the culture medium.
Heredia
S
S
Departamento de Microbiología I, Facultad de Medicina, Universidad Complutense de Madrid.
Ramos
C
C
Gómez-Lus
M L
ML
Prieto
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion; D002462:Cell Membrane / Q000502:physiology; D005936:Glucans / Q000096:biosynthesis; D008826:Microbial Sensitivity Tests; D011137:Polystyrenes; D016986:Streptococcus sobrinus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D014867:Water
D000900:Anti-Bacterial Agents; D005936:Glucans; D011137:Polystyrenes; D014867:Water
Efecto de diferentes antimicrobianos a concentraciones subinhibitorias sobre la producción de glucano insoluble, adherencia al poliestireno ehidrofobicidad de superficie bacteriana de Streptococcus sobrinus.