Edema factor from the venom of Trimeresurus elegans (Sakishimahabu).
An edema factor was isolated from the venom of Trimeresurus elegans using HW-55, CM-Cellulose, and Mono S column chromatographies. Homogeneity was demonstrated by the formation of a single band in polyacrylamide gel electrophoresis (pH 8.3). The edema factor has a molecular weight of 25,500, an isoelectric point of 7.5, and express edema, proteolytic and capillary permeability-increasing activities. Edema, proteolytic and capillary permeability-increasing activities are inhibited by ethylenediaminetetraacetic acid (EDTA), o-phenanthroline, and N-bromosuccinimide. Additionally, this factor exhibits kinin-releasing activity. The edema factor possesses proteolytic activity as shown by hydrolyzing the Val(3)-Asn(4), His(5)-Leu(6), Ser(9)-His(10), Ala(14)-Leu(15), Leu(15)-Tyr(16), Tyr(16)-Leu(17), and Glu(21)-Arg(22) bonds of oxidized insulin B chain. The A alpha, B beta, and gamma chains of human fibrinogen were also hydrolyzed. The edema factor was found to contain 1 mol of zinc and 2 mols of calcium per mol of protein and the amino-terminal sequence was determined.
Nikai
T
T
Faculty of Pharmacy, Meijo University, Nagoya, Japan. nikai@meijo-u.ac.jp
Suzuki
Y
Y
Komori
Y
Y
Sugihara
H
H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003435:Crotalid Venoms / Q000737:chemistry*; D004487:Edema / Q000139:chemically induced*; D010450:Endopeptidases / Q000737:chemistry; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017841:Trimeresurus; D014757:Viper Venoms / Q000737:chemistry*
D000596:Amino Acids; D003435:Crotalid Venoms; C029748:Trimeresurus venoms; D014757:Viper Venoms; C416405:edema factor; D010450:Endopeptidases; C121739:elegatoxin