title: Biotechnology for the production of nutraceuticals enriched in conjugated linoleic acid: II. Multiresponse kinetics of the hydrolysis of corn oil by a Pseudomonas sp. lipase immobilized in a hollow-fiber reactor.
abstract: The hydrolysis of corn oil in the presence of a lipase from Pseudomonas sp. immobilized within the walls of a hollow-fiber reactor was studied at 30 degrees C. To assess the selectivity of this immobilized enzyme, the effluent concentrations of five different free fatty acids were measured using high-performance liquid chromatography (HPLC). Several rate expressions associated with a generic ping-pong bi-bi mechanism were used to fit the experimental data for this lipase-catalyzed reaction. A multiresponse nonlinear regression method was employed to determine the kinetic parameters associated with these rate expressions. Quasi-optimum operating conditions corresponded to 30 degrees C and a buffer pH value of 7.0. Under these conditions, the concentration of free linoleic acid (C18:2) (the fatty acid of primary interest) in the effluent oil stream for a fluid residence time of 6 h was approximately 0.5 M.
authors.affiliation: Department of Chemical Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, USA.
authors.forename: P S
authors.initials: PS
authors.lastname: Sehanputri
authors.forename: C G
authors.initials: CG
authors.lastname: Hill
mesh_terms: D019149:Bioreactors; D003314:Corn Oil / Q000378:metabolism*; D004800:Enzymes, Immobilized; D006868:Hydrolysis; D007700:Kinetics; D019787:Linoleic Acid / Q000096:biosynthesis* / Q000737:chemistry; D008049:Lipase / Q000378:metabolism; D008954:Models, Biological; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D003314:Corn Oil; D019787:Linoleic Acid; D008049:Lipase