Assessing the allozyme variation in cultivars and Swedish landraces of rye (Secale cereale L.).
Genetic interpretation and diversity of 9 isozyme loci have been estimated in 7 improved varieties and 19 landraces from Sweden by means of starch gel electrophoresis. The isozyme systems were ACO, DIA, GPI, MDH, PGD and PGM. For the statistic analysis we used the following measures: average number of alleles per locus, percentage of polymorphic loci, average heterozygosity direct count and average heterozygosity Hardy-Weinberg expected unbiased estimate. The measures were made on species and population levels. The distribution of the total genetic diversity among populations was also calculated. To illustrate the genetic relationships among populations, genetic distances were measured and principal component analysis performed. As expected in a cross-pollinated crop we found high genetic diversity and a larger variation within than among the populations. Somewhat unexpectedly, however, we found that the currently used varieties have the same high level of heterozygosity as the landraces but in the dendrogram the two groups are separated. The dendrogram showed three main clusters. The large cluster included 21 populations and the two small clusters were clearly distinguishable from the rest. The landrace spring-type could not be separated from the landraces winter-type, but we did detect a difference between different spring types. A few populations had unique alleles for certain loci.
Persson
K
K
Department of Plant Breeding Research, Swedish University of Agricultural Sciences (SLU), Svalöv, Sweden. karin.persson@vf.slu.se
von Bothmer
R
R
D000154:Aconitate Hydratase / Q000235:genetics; D000483:Alleles; D016000:Cluster Analysis; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics; D004592:Electrophoresis, Starch Gel; D014644:Genetic Variation; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D008291:Malate Dehydrogenase / Q000235:genetics; D008957:Models, Genetic; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012434:Secale / Q000235:genetics*; D013548:Sweden
D007527:Isoenzymes; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase; D000154:Aconitate Hydratase; D005956:Glucose-6-Phosphate Isomerase