Human herpesvirus 8-encoded interleukin-6 homologue (viral IL-6) induces endogenous human IL-6 secretion.
We found that human herpesvirus 8-encoded IL-6 (vIL-6) induced endogenous human IL-6 (huIL-6) secretion from various cell lines (MT-4, THP-1, U937, Raji, and CESS) including patients with multicentric Castleman's disease. Especially, in MT-4 cells, huIL-6 was enhanced with vIL-6 by 30-fold compared with that of control. In addition, reverse transcriptase-polymerase chain reaction confirmed that vIL-6 induced huIL-6 expression in MT-4 cells. Our novel finding of the huIL-6 induction by vIL-6 indicates that vIL-6 may play a significant role in the pathogenesis of HHV-8 associated diseases.
Mori
Y
Y
Department of Microbiology, Osaka University Medical School, Osaka University Medical School, Osaka University, Osaka, Japan. ymori@micro.med.osaka-u.ac.jp
Nishimoto
N
N
Ohno
M
M
Inagi
R
R
Dhepakson
P
P
Amou
K
K
Yoshizaki
K
K
Yamanishi
K
K
D000818:Animals; D005871:Castleman Disease / Q000276:immunology; D002460:Cell Line; D006566:Herpesviridae Infections / Q000276:immunology; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology*; D051379:Mice; D012514:Sarcoma, Kaposi / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000494:pharmacology*
D015850:Interleukin-6; D014764:Viral Proteins