alpha-Amylase production by free and immobilized Bacillus subtilis.
The effect of glucose on the alpha-amylase production by Bacillus subtilis ATCC-21556 was studied. Initial glucose concentrations up to 20 g/L were found to be directly proportional to the specific alpha-amylase production in an immobilized-cell batch system, whereas a free-cell batch system presented an inversely proportional relationship with the initial glucose concentration. This might be owing to the alpha-amylase repression by the glucose present in the culture medium. Three hundred eighty-five percent of the specific alpha-amylase production with the free-cell system was produced by the immobilized-cell batch culture.
Duran-Paramo
E
E
Laboratoire de Technologie Enzymatique (UPRES-A CNRS 6022), Université de Technologie de Compiègne, France.
Garcia-Kirchner
O
O
Hervagault
J F
JF
Thomas
D
D
Barbotin
J N
JN
D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D018533:Biomass; D018914:Cells, Immobilized / Q000187:drug effects / Q000201:enzymology*; D004790:Enzyme Induction; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D000516:alpha-Amylases / Q000096:biosynthesis*
D000516:alpha-Amylases; D005947:Glucose