Factors associated with the formation of triploid zygotes after intracytoplasmic sperm injection.
OBJECTIVE
To determine whether clinical or laboratory factors influence development of triploid (3PN) zygotes after ICSI.


METHODS
Retrospective review.


METHODS
The assisted reproductive technology program of Brigham and Women's Hospital.


METHODS
Patients undergoing ICSI.


METHODS
None.


METHODS
Cycles were divided into two groups: group A, cycles with one or more 3PN zygotes after ICSI, and group B, cycles with no 3PN zygotes. Age, amount of gonadotropin administered, peak estradiol levels, number of follicles, number of oocytes retrieved and injected, time between retrieval and injection, oocyte abnormalities, sperm type and motile count, percentage of diploid zygotes, and ongoing pregnancy rates were compared between groups.


RESULTS
Compared with patients in group B, those in group A received fewer ampoules of gonadotropins, had higher estradiol levels, and had more follicles on the day of hCG administration, oocytes, immature oocytes and oocytes injected and lower percentages of diploid zygotes. However, ongoing pregnancy rates did not differ between groups.


CONCLUSIONS
Patients who produce 3PN zygotes after ICSI are high responders to ovarian stimulation. The appearance of such embryos is not associated with lower ongoing pregnancy rates and should not necessarily dictate alterations in ovarian stimulation protocols.
Sachs
A R
AR
Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
Politch
J A
JA
Jackson
K V
KV
Racowsky
C
C
Hornstein
M D
MD
Ginsburg
E S
ES
D000328:Adult; D016922:Cellular Senescence; D006063:Chorionic Gonadotropin / Q000627:therapeutic use; D015925:Cryopreservation; D004171:Diploidy; D004958:Estradiol / Q000097:blood; D005260:Female; D006062:Gonadotropins / Q000008:administration & dosage / Q000627:therapeutic use; D006801:Humans; D009865:Oocytes / Q000502:physiology; D006080:Ovarian Follicle / Q000033:anatomy & histology; D011003:Ploidies; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D015053:Zygote / Q000502:physiology*
D006063:Chorionic Gonadotropin; D006062:Gonadotropins; D004958:Estradiol