Isolation and characterization of a jacalin-related mannose-binding lectin from salt-stressed rice (Oryza sativa) plants.
A novel plant lectin was isolated from salt-stressed rice (Oryza sativa L.) plants and partially characterized. The lectin occurs as a natural mixture of two closely related isoforms consisting of two identical non-covalently linked subunits of 15 kDa. Both isoforms are best inhibited by mannose and exhibit potent mitogenic activity towards T-lymphocytes. Biochemical analyses and sequence comparisons further revealed that the rice lectins belong to the subgroup of mannose-binding jacalin-related lectins. In addition, it could be demonstrated that the lectins described here correspond to the protein products of previously described salt-stress-induced genes. Our results not only identify the rice lectin as a stress protein but also highlight the possible importance of protein-carbohydrate interactions in stress responses in plants.
Zhang
W
W
Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium.
Peumans
W J
WJ
Barre
A
A
Astoul
C H
CH
Rovira
P
P
Rougé
P
P
Proost
P
P
Truffa-Bachi
P
P
Jalali
A A
AA
Van Damme
E J
EJ
D000595:Amino Acid Sequence; D000818:Animals; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism*; D037241:Mannose-Binding Lectins; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012275:Oryza / Q000378:metabolism* / Q000502:physiology; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D011817:Rabbits; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000378:metabolism*
D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C043696:jacalin; C417580:orysata protein, Oryza sativa; D012965:Sodium Chloride; D008358:Mannose