[The use of polymerase chain reaction for detection of tetracycline resistance urogenital mycoplasma ].
PCR was used for detecting tetracyclin resistance genes tet-M and tet-O in Mollicutes. The tet determinants were detected in U. urealyticum and M. hominis genomes in 60 and 57.14%, respectively. PCR was used to detect urogenital mycoplasma in patients with different urogenital infections. The rate of detection of U. urealyticum was 10.71-25.1% in males and 23.08-61.91% in females, for M. hominis these values were 6.25-11.45% and 3.85-22.99%, and for M. genitalium 6.25-29.64% and 4.76-30.0%, respectively. Hence, the detection rate of tet determinants in mycoplasma by PCR was high (7-60%) in all groups of patients.
Solov'eva
S V
SV
Tsoĭ
E G
EG
Zigangirova
N A
NA
Gamova
N A
NA
Rakovskaia
I V
IV
Gintsburg
A L
AL
D005260:Female; D052776:Female Urogenital Diseases / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013753:Tetracycline Resistance
Primenenie polimeraznoĭ tsepnoĭ reaktsii dlia vyiavleniia teratsiklinustochivykh shtammov urogenital'nykh mikoplazm.