[An immunoglobulin preparation as a calibrating agent for the gel filtration method].
The fractional composition of commercial immunoglobulin was studied by the method of gel filtration on ultragel AcA-34 and the possibility of its use for the calibration of a chromatographic column was shown. The fractionation of a specimen of IgG revealed the presence of 4 fractions. Their molecular weights corresponded to dimers, monomers, Fab fragments and low-molecular peptides. The qualitative fractional composition of the preparation was shown to be stable during 3 years of storage, as well as after keeping the preparation at 37 degrees C for 1 month. The attested characteristic of each IgG fraction, the distribution coefficient (Kav), was established. The use of a specimen of immunoglobulin obtained with the use of the modified Cohn's method--as calibrant will make it possible to calibrate the columns for a shorter period and to control the correctness of the analysis.
Kargina
T M
TM
Tarasevich Research Institute for Standardization and Control of Medical Biological Preparations, Moscow, Russia.
Volkova
R A
RA
Gavrilenkova
V Iu
VIu
D002138:Calibration / Q000592:standards; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004355:Drug Stability; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D008970:Molecular Weight; D013997:Time Factors
D007074:Immunoglobulin G
Preparat immunoglobulina v kachestve kalibranta dlia metoda gel-fil'tratsii.