Sensory afferents and motor neurons as targets for nitric oxide in the locust.
In the adult locust, nitric oxide (NO) synthase is expressed in interneurons that innervate mechanosensory neuropils, indicating that NO may participate in mechanosensory processing. Here, we have identified potential neuronal targets of NO by localizing the expression and activity of soluble guanylyl cyclase (SGC), its principal molecular target in the nervous system. We used two complementary approaches, namely immunolocalization of SGC alpha-subunit (SGCalpha), and of cyclic GMP (cGMP) after exposure to an NO donor. The cell bodies, axons and central projections of thoracic exteroceptors, proprioceptors, auditory receptors, and chemoreceptors were strongly immunoreactive for SGCalpha. Strong SGCalpha immunoreactivity also occurred in all thoracic motor neurons, including their axon terminals. NO-donors induced a pattern of cGMP immunostaining that was similar to the distribution of SGCalpha, indicating that both sensory and motor neurons contain functional SGC. Therefore, NO may modulate both the input from these sensory neurons and the output of motor neurons. Although the expression of SGCalpha was highly consistent, NO donors did not always induce cGMP-staining in SGC-containing neurons, suggesting that SGC is coregulated by factors other than NO. Complementing previous reports in the visual and olfactory system, our results indicate a general role for NO-cGMP signaling in early sensory processing; diffusible signals may mediate a cross-adaptation or -sensitization within neural maps where similarly tuned neurons have adjacent projections, an anatomical arrangement shared by many sensory systems.
Ott
S R
SR
Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, United Kingdom. S.R.Ott@gmw.ac.uk
Jones
I W
IW
Burrows
M
M
Elphick
M R
MR
D000344:Afferent Pathways / Q000737:chemistry* / Q000201:enzymology; D000818:Animals; D006152:Cyclic GMP / Q000032:analysis; D005260:Female; D006110:Grasshoppers / Q000737:chemistry / Q000201:enzymology; D006162:Guanylate Cyclase / Q000032:analysis*; D009046:Motor Neurons / Q000737:chemistry* / Q000201:enzymology; D009475:Neurons, Afferent / Q000737:chemistry* / Q000201:enzymology; D009569:Nitric Oxide / Q000502:physiology*
D009569:Nitric Oxide; D006162:Guanylate Cyclase; D006152:Cyclic GMP