Relationship between viral load in blood, cerebrospinal fluid, brain tissue and isolated microglia with neurological disease in macaques infected with different strains of SIV.
The role of the viral burden in the brain for the pathogenesis of human immunodeficiency virus-associated neurological disorders is still unclear. To address this issue, we have quantified the viral load in plasma, cerebrospinal fluid (CSF) and brain tissue of macaques infected with simian immunodeficiency virus (SIV). We discovered that the viral strain used for infection determines the replicative capacity in microglial cells as well as the extent of neuropathological lesions and the occurrence of neurological symptoms. Moreover, the viral load in the brain parenchyma correlated with the development of overt neurological disease whereas the one in plasma did not. By comparing the viral load in three different compartments, we demonstrated that the viral burden in the CSF is influenced both by the viral replication in the periphery as well as in the brain parenchyma. According to these results, it is not the absolute amount of viral load in the CSF but rather the viral antigen contributed by the viral production within the brain which correlates with the development of neurological disease. In longitudinal studies, we observed that this autochthonous virus production, as evidenced by a ratio of the viral load in CSF to the one in plasma, takes place for a prolonged period of time before overt neurological signs are manifested. This finding suggests that this ratio could be used as a prognostic marker for immunodeficiency virus-induced neurological disease.
Demuth
M
M
Institut für Virologie und Immunbiologie, Julius-Maximilians-Universität, Versbacherstrabetae 7, D-97078 Würzburg, Germany.
Czub
S
S
Sauer
U
U
Koutsilieri
E
E
Haaft
P
P
Heeney
J
J
Stahl-Hennig
C
C
ter Meulen
V
V
Sopper
S
S
D000386:AIDS-Related Complex / Q000134:cerebrospinal fluid / Q000175:diagnosis; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D001921:Brain / Q000473:pathology / Q000821:virology*; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D004195:Disease Models, Animal; D018792:Encephalitis, Viral / Q000097:blood / Q000134:cerebrospinal fluid / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D015683:Gene Products, gag / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D017403:In Situ Hybridization; D008253:Macaca mulatta; D017628:Microglia / Q000821:virology*; D011379:Prognosis; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000134:cerebrospinal fluid / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D019562:Viral Load; D014779:Virus Replication
D000956:Antigens, Viral; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D012367:RNA, Viral