Stage-specific protein synthesis during early embryogenesis in Drosophila melanogaster.
The changes in protein species synthesized during early Drosophila embryogenesis were characterized by two-dimensional electrophoresis. Of the 261 proteins scored, 68 (26%) show dramatic changes in rates of synthesis during the first 8 h of embryogenesis. These stage-specific proteins can be classified into three categories: early, detected at 1, 2 and 3 h but not later; late, not detected at 1 h, but appearing later; and discontinuous, detected before and after, but not at 3 and 4 h. RNA was extracted from three representative stages, translated in vitro, and the translation products separated on two-dimensional gels. There was a strong correlation between the patterns of synthesis in vivo and in vitro, suggesting that the early proteins are translated from maternal mRNA, and the late proteins from zygotic mRNA. A thorough comparison was made between the proteins synthesized in wild-type and dorsal embryos, in which virtually only dorsal hypoderm differentiates. The first observed difference was a reduced synthesis of actin I at 8 h, indicating that the absence of mesodermal and endodermal tissues is not detectable at the level of moderately abundant protein until the onset of differentiation.
Trumbly
R J
RJ
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, U.184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Institut de Chimie Biologique, Strasbourg, France.
Jarry
B
B
D000818:Animals; D002474:Cell-Free System; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology* / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000502:physiology; D047108:Embryonic Development; D005260:Female; D019476:Insect Proteins / Q000096:biosynthesis*; D016296:Mutagenesis; D009687:Nuclear Proteins / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D014157:Transcription Factors
D029721:Drosophila Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; C062236:dl protein, Drosophila; D012313:RNA