title: Two bean cell wall proteins more abundant during water deficit are high in proline and interact with a plasma membrane protein.
abstract: Two antigenically related glycoproteins, called p33 and p36, accumulate in the soluble fraction of the cell wall in response to water deficit in Phaseolus vulgaris. In this report, we show that p33 and p36 are able to adhere to leaf protoplasts, and that they bind to plasma membrane (PM) vesicles in a divalent cation-dependent manner. Data from the partial amino acid sequence of the p33 and p36 proteins indicate that they contain repeats of the decapeptide POVYKPOVEK; therefore, they are related to proline-rich proteins. Binding assays demonstrate that both proteins specifically bind to an 80 kDa PM protein. This binding is competed with a peptide that contains the RGD motif, as well as with fibronectin, which also includes this sequence, suggesting that the 80 kDa PM protein has an integrin-like function whose natural ligands are p33 and p36. This is the first case where a PM ligand for a higher plant cell wall protein has been identified.
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250 Cuernavaca, Morelos, México.
authors.forename: B I
authors.initials: BI
authors.lastname: García-Gómez
authors.forename: F
authors.initials: F
authors.lastname: Campos
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: A A
authors.initials: AA
authors.lastname: Covarrubias
mesh_terms: D000595:Amino Acid Sequence; D002473:Cell Wall / Q000378:metabolism*; D007887:Fabaceae / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D010946:Plants, Medicinal; D011392:Proline / Q000378:metabolism*; D011485:Protein Binding; D014867:Water
chemical_list: D008565:Membrane Proteins; D009842:Oligopeptides; D014867:Water; C047981:arginyl-glycyl-aspartic acid; D011392:Proline