Molecular cloning of a female-specific cDNA with unique repeat sequences from the fat body of the adult locust, Locusta migratoria.
A cDNA clone encoding a 25-kDa protein (25K) was isolated from a cDNA library made from RNA isolated from the adult fat body and ovaries of the locust, Locusta migratoria. The longest open reading frame of this cDNA clone encodes a 225-amino acid polypeptide, the N-terminal end of which was similar to the 21-kDa and 19-kDa juvenile hormone induced proteins identified in the locust hemolymph, but the C-terminal end was different. The C-terminal end of the 25K cDNA contained seven unique repeat elements of 10 amino acids each, most of which are polar residues. Expression of the 25K mRNA was tissue-, development- and sex-specific. A 1.2-kb mRNA was detected using the 25K cDNA as a probe only in the fat body of adult females. The mRNA started to appear at day 4 after the insect molted to the adult and rapidly increased by day 6. The mRNA was absent in the ovarian follicle cells and fat body of adult males. In vitro transcription and translation of the 25K cDNA produced a protein that migrated around 32 kDa on sodium dodecyl sulfate polyacrylamide gels. The 25K cDNA was expressed in a baculovirus expression system and the protein produced also migrated around 32 kDa.
Feng
Q
Q
Department of Biology, York University, 4700 Keele Street, North York, M3J 1P3, Ontario, Canada.
Palli
S R
SR
Ladd
T R
TR
Sohi
S S
SS
Retnakaran
A
A
Davey
K G
KG
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005216:Fat Body / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D017344:Genes, Insect; D005822:Genetic Vectors; D006110:Grasshoppers / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D011485:Protein Binding; D014176:Protein Biosynthesis; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012717:Sesquiterpenes / Q000378:metabolism
D018076:DNA, Complementary; D012717:Sesquiterpenes; C036585:juvenile hormone III