Oral administration of branched chain amino acids improves virus-induced glucose intolerance in mice.
We investigated the therapeutic effect of branched chain amino acids (BCAA) on mice with glucose intolerance induced by encephalomyocarditis virus (EMCV). Male DBA/2 mice were divided into three groups: treated with BCAA, (such as valine, leucine, and isoleucine), untreated, and control. BCAA-treated and -untreated groups were inoculated intraperitoneally with the NDK25 variant of EMCV at 200 plaque-forming units per mouse. The BCAA-treated group was administered orally 0.9 g/kg/day of each BCAA from the day after viral inoculation. The control group neither received virus inoculation nor was treated with BCAA. One week after inoculation, oral glucose tolerance tests (OGTT) were performed. After the glucose loading at 1.5 g/kg of body weight, blood glucose levels in the untreated group were 92.0+/-10.0 mg/dl at baseline, 224.6+/-10.9 mg/dl at 30 min, and 169.4+/-21.4 mg/dl at 60 min, which were significantly (P<0.05) higher than those in the control group (62. 7+/-3.6 mg/dl, 167.2+/-16.4, and 83.8+/-6.0 mg/dl, respectively). Blood glucose levels in the BCAA-treated group were 54.5+/-3.7 mg/dl at baseline, 145.2+/-8.7 mg/dl at 30 min, and 128.7+/-18.3 mg/dl at 60 min after the glucose loading, which were not significantly higher than those in the control group. Immunoreactive insulin levels at 30 min after the glucose loading were lower in the untreated group than in the control group at 1 week after virus inoculation. Histological investigations showed that the grade of insulitis in the pancreas of mice of the BCAA-treated group was lower than that of the mice of the untreated group. These results suggest that oral administration of BCAA is able to improve glucose intolerance induced by EMCV.
Utsugi
T
T
Second Department of Internal Medicine, Gunma University, School of Medicine, 3-39-11 Showa-machi, Gunma 371, Maebashi, Japan.
Yoshida
A
A
Kanda
T
T
Kobayashi
I
I
Kurabayashi
M
M
Tomono
S
S
Kawazu
S
S
Tajima
Y
Y
Nagai
R
R
D000284:Administration, Oral; D000597:Amino Acids, Branched-Chain / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D018188:Cardiovirus Infections; D004686:Encephalomyocarditis virus; D018149:Glucose Intolerance / Q000188:drug therapy* / Q000821:virology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010179:Pancreas / Q000187:drug effects / Q000473:pathology
D000597:Amino Acids, Branched-Chain; D001786:Blood Glucose