Comparison of two colorimetric assays to determine viral infectivity in micro culture virus titration.
Efficacy of two colorimetric assays, viz. MTT (3-4,5-dimethylthiazol-2-(yl-2,5-diphenyl tetrazolium bromide) and neutral red (NR) assays, performed by integrating them to micro culture virus titration (MCVT), was compared with the conventional MCVT method in terms of percentages of infectivity and 50% infectivity end points by employing Polio virus type-3 and Dengue virus type 4 as the candidate viruses. The results suggested that MTT assay has an edge over NR assay as well as conventional MCVT method. For the first time, NR assay has been successfully employed for the determination of virus infectivity titre.
Parida
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MM
Division of Virology, Defence Research & Development Establishment, Gwalior, India.
Pandya
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G
Bhargava
R
R
Bhattacharya
R
R
Jana
A M
AM
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003124:Colorimetry / Q000379:methods*; D003588:Cytopathogenic Effect, Viral; D003716:Dengue Virus / Q000472:pathogenicity; D005069:Evaluation Studies as Topic; D006801:Humans; D009499:Neutral Red; D017955:Poliovirus / Q000472:pathogenicity; D013778:Tetrazolium Salts; D013844:Thiazoles; D014709:Vero Cells; D014773:Virology / Q000379:methods*
D013778:Tetrazolium Salts; D013844:Thiazoles; D009499:Neutral Red; C022616:thiazolyl blue