FACKEL is a sterol C-14 reductase required for organized cell division and expansion in Arabidopsis embryogenesis.
In flowering plants, the developing embryo consists of growing populations of cells whose fates are determined in a position-dependent manner to form the adult organism. Mutations in the FACKEL (FK) gene affect body organization of the Arabidopsis seedling. We report that FK is required for cell division and expansion and is involved in proper organization of the embryo. We isolated FK by positional cloning. Expression analysis in embryos revealed that FK mRNA becomes localized to meristematic zones. FK encodes a predicted integral membrane protein related to the vertebrate lamin B receptor and sterol reductases across species, including yeast sterol C-14 reductase ERG24. We provide functional evidence that FK encodes a sterol C-14 reductase by complementation of erg24. GC/MS analysis confirmed that fk mutations lead to accumulation of intermediates in the biosynthetic pathway preceding the C-14 reductase step. Although fk represents a sterol biosynthetic mutant, the phenotype was not rescued by feeding with brassinosteroids (BRs), the only plant sterol signaling molecules known so far. We propose that synthesis of sterol signals in addition to BRs is important in mediating regulated cell growth and organization during embryonic development. Our results indicate a novel role for sterols in the embryogenesis of plants.
Schrick
K
K
Zentrum f]ur Molekularbiologie der Pflanzen, Universit]at T]ubingen, D-72076 T]ubingen, Germany. kathrin.schrick@uni-tuebingen.de
Mayer
U
U
Horrichs
A
A
Kuhnt
C
C
Bellini
C
C
Dangl
J
J
Schmidt
J
J
Jürgens
G
G
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000196:embryology* / Q000201:enzymology; D001483:Base Sequence; D002455:Cell Division; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D008401:Gas Chromatography-Mass Spectrometry; D005816:Genetic Complementation Test; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010088:Oxidoreductases / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic
D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D010088:Oxidoreductases; C022672:delta(14)-sterol reductase