Absorption and metabolism of genistin in the isolated rat small intestine.
Uptake and intestinal metabolism of physiologically active genistin were studied in an ex vivo intestinal perfusion model; luminally applied concentrations were 5.9, 12.0, and 23.8 micromol/l. The intestinal absorption of genistin was 14.9% (+/-2.3, n=9), irrespective of the amounts applied. The majority of the absorbed genistin appeared as genistein glucuronide (11.6%), also recovered as the main metabolite on the luminal side (19.5%). Minor amounts of genistin (1.3%) and genistein (1.9%) were found on the vascular side, whereas 15.4% of applied genistin was luminally cleaved to yield genistein. Sulfate derivatives of genistein or genistin were not observed.
Andlauer
W
W
Institute for Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart, Germany. andlauer@uni-hohenheim.de
Kolb
J
J
Fürst
P
P
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004968:Estrogens, Non-Steroidal / Q000493:pharmacokinetics*; D019833:Genistein / Q000378:metabolism; D020723:Glucuronic Acid / Q000378:metabolism; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism*; D007529:Isoflavones / Q000493:pharmacokinetics*; D008297:Male; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D004968:Estrogens, Non-Steroidal; D007529:Isoflavones; C040641:genistin; D020723:Glucuronic Acid; D019833:Genistein