Changes in superoxide dismutase activity and mRNA in vivo after short-term supplementation with trilinolein in rats.
BACKGROUND
Oxidative damage plays a central role in atherogenesis and antioxidation defense mechanisms may prevent atherosclerosis. This study evaluated the effect of short-term supplementation of the natural lipophilic antioxidant trilinolein on superoxide dismutase (SOD) activity and SOD-mRNA gene expression in vivo in rat vital organs.


METHODS
Male Wistar rats (n = 8) were injected intraperitoneally with trilinolein (1 mM/ml/kg/day in 0.5% ethanol) daily for three consecutive days. Two control groups (n = 8) were administered saline or 0.5% ethanol in saline, respectively, for three days.


RESULTS
Assay of SOD activity and SOD-mRNA by Northern blotting in rat liver, spleen and brain showed significant increases in SOD activity and increased SOD-mRNA gene expression.


CONCLUSIONS
The natural lipophilic antioxidant trilinolein potentiates the SOD antioxidation defense mechanism and increases gene expression of SOD-mRNA after short-term supplementation in rats.
Chan
P
P
Department of Medicine, Taipei Medical College, Taiwan, ROC.
Huang
W P
WP
Liu
J C
JC
Chen
Y J
YJ
Cheng
J T
JT
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005609:Free Radicals; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000494:pharmacology*
D000975:Antioxidants; D005609:Free Radicals; D012333:RNA, Messenger; D014280:Triglycerides; D013482:Superoxide Dismutase; C009564:trilinolein