title: Applications of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones.
abstract: Plastic embedding has been used to localize various antigens in conjunction with immunohistochemistry. Peptide hormones have been among the antigens that have been studied extensively. Recent application of water-soluble plastics such as LR White and Lowicryl has extended the ranges of detectable antigens and enabled the observation of antigen-antigen or mRNA-antigen combinations. This review article deals with technical aspects, procedures, and applications in endocrine cells.
authors.affiliation: Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan. osamura@is.icc.u-tokai.ac.jp
authors.forename: R Y
authors.initials: RY
authors.lastname: Osamura
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: A
authors.initials: A
authors.lastname: Matsuno
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism; D004703:Endocrine System / Q000166:cytology / Q000378:metabolism; D006728:Hormones / Q000096:biosynthesis* / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D016253:Microscopy, Immunoelectron / Q000379:methods; D010452:Peptide Biosynthesis; D010455:Peptides / Q000378:metabolism*; D016613:Plastic Embedding
chemical_list: D000180:Acrylic Resins; D006728:Hormones; D010455:Peptides; C048707:LR white