title: Improved operational stability of peroxidases by coimmobilization with glucose oxidase.
abstract: The operational stability of peroxidases was considerably enhanced by generating hydrogen peroxide in situ from glucose and oxygen. For example, the total turnover number of microperoxidase-11 in the oxidation of thioanisole was increased sevenfold compared with that obtained with continuous addition of H(2)O(2). Coimmobilization of peroxidases with glucose oxidase into polyurethane foams afforded heterogeneous biocatalysts in which the hydrogen peroxide is formed inside the polymeric matrix from glucose and oxygen. The total turnover number of chloroperoxidase in the oxidation of thioanisole and cis-2-heptene was increased to new maxima of 250. 10(3) and 10. 10(3), respectively, upon coimmobilization with glucose oxidase. Soybean peroxidase, which normally shows only classical peroxidase activity, was transformed into an oxygen-transfer catalyst when coimmobilized with glucose oxidase. The combination catalyst mediated the enantioselective oxidation of thioanisole [50% ee (S)] with 210 catalyst turnovers.
authors.affiliation: Laboratory of Organic Chemistry and Catalysis, Delft University of Technology, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: van de Velde
authors.forename: N D
authors.initials: ND
authors.lastname: Lourenço
authors.forename: M
authors.initials: M
authors.lastname: Bakker
authors.forename: F
authors.initials: F
authors.lastname: van Rantwijk
authors.forename: R A
authors.initials: RA
authors.lastname: Sheldon
mesh_terms: D002711:Chloride Peroxidase / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized; D005658:Fungi / Q000201:enzymology; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D005949:Glucose Oxidase / Q000378:metabolism*; D006536:Heptanes / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D010544:Peroxidases / Q000378:metabolism*; D011140:Polyurethanes; D012996:Solutions; D013025:Glycine max / Q000201:enzymology; D013237:Stereoisomerism; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism; D020002:tert-Butyl Alcohol / Q000494:pharmacology
chemical_list: D004800:Enzymes, Immobilized; D006536:Heptanes; D011140:Polyurethanes; D012996:Solutions; D013440:Sulfides; C028279:polyurethane foam; C093850:methylphenylsulfide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D010544:Peroxidases; C021799:microperoxidase; D002711:Chloride Peroxidase; D005947:Glucose; D020002:tert-Butyl Alcohol; D010100:Oxygen