Efficacy of a potentized homoeopathic drug (Arsenicum-album-30) in reducing cytotoxic effects produced by arsenic trioxide in mice: III. Enzymatic changes and recovery of tissue damage in liver.
OBJECTIVE
To determine whether the potentized homoeopathic drug Arsenicum Album-30 can induce enzymatic and some other biochemical changes to repair tissue damage caused by the injection of arsenic trioxide in mice.


METHODS
Controlled laboratory study.


METHODS
Mice injected with arsenic trioxide and then orally administered the homoeopathic drug were compared with control animals who either received saline only, or injections of arsenic trioxide, or injections of arsenic trioxide followed by orally administered dilute alcohol. Activities of the enzymes acid and alkaline phosphatases, lipid peroxidation and reduced glutathione, which are used as 'marker' enzymes for cytotoxicity levels, were assessed by standard methods. Histopathological slide preparations of liver were made by routine microtechnique method of tissue sectioning and staining with haematoxylin- eosin for histological examination.


RESULTS
The mice fed homoeopathic drug showed positive results of tissue recovery both in terms of enzymatic and histological changes, compared to controls.


CONCLUSIONS
The homoeopathic drug is capable of preventing or repairing liver damage induced by arsenic trioxide and the positive changes were also confirmed by the activities of the enzymatic markers.
Kundu
S N
SN
Department of Zoology, Kalyani University, Kalyani, 741235, India.
Mitra
K
K
Bukhsh
A R
AR
D000818:Animals; D020261:Arsenic Poisoning / Q000188:drug therapy*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000009:adverse effects* / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury; D008107:Liver Diseases / Q000201:enzymology / Q000473:pathology; D008419:Materia Medica / Q000627:therapeutic use*; D051379:Mice; D010087:Oxides / Q000009:adverse effects*
D001152:Arsenicals; D008419:Materia Medica; D010087:Oxides; C121243:arsenicum album-30; D000077237:Arsenic Trioxide