Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family.
We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family. The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus.
Aapola
U
U
Institute of Medical Technology, University of Tampere, Finland. ulla.aapola@uta.fi
Kawasaki
K
K
Scott
H S
HS
Ollila
J
J
Vihinen
M
M
Heino
M
M
Shintani
A
A
Kawasaki
K
K
Minoshima
S
S
Krohn
K
K
Antonarakis
S E
SE
Shimizu
N
N
Kudoh
J
J
Peterson
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000090205:DNA Methyltransferase 3A; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D016335:Zinc Fingers / Q000235:genetics*
C000717967:DNMT3A protein, human; C000715535:Dnmt3a protein, mouse; C412484:DNMT3L protein, human; C498796:Dnmt3l protein, mouse; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A