Comparison of serotyping, ribotyping and pulsed-field gel electrophoresis for distinguishing group A Streptococcus strains isolated in Albania.
Conventional serotyping for T antigens, rRNA gene restriction fragment length polymorphism analysis (ribotyping) and pulsed-field gel electrophoresis were compared for distinguishing among group A streptococci isolated in Albania between 1980-1982 and in 1995. A total of twelve serotypes were identified among seventy GAS strains. Ribotyping revealed eight and eleven distinct patterns after digestion with HindIII and PvuII, respectively. Twenty-three strains of serotype T12 were subdivided in 10 ribotypes and 11 strains of T2 serotype were differentiated in 5 ribotypes. By comparison, PFGE generated 37 patterns after SmaI digestion. The index of discrimination, using the Hunter-Gaston formula, was applied to assess the value of these methods for interpretation of the epidemiological data. For serotyping the value of index was 0.85. The ribotyping system revealed an ID of 0.83 when the combination HindIII and PvuII was used. This index reached 0.97 for PFGE. The methods used were useful to subtype the isolates of GAS.
Shundi
L
L
Cantacuzino Institute Bucharest, Molecular Epidemiology Laboratory, Romania.
Surdeanu
M
M
Damian
M
M
D000415:Albania / Q000453:epidemiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007169:Impetigo / Q000382:microbiology; D010031:Otitis / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D012541:Scarlet Fever / Q000382:microbiology; D012703:Serotyping; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000145:classification* / Q000302:isolation & purification; D014069:Tonsillitis / Q000382:microbiology
D004269:DNA, Bacterial