title: Capillary electrophoresis with postcolumn infectivity assay for the analysis of different serotypes of human rhinovirus (common cold virus).
abstract: Differentiation of virus serotypes with capillary zone electrophoresis was demonstrated. For four serotypes of human rhinovirus (HRV2, HRV14, HRV16, HRV49), different electrophoretic mobility was achieved at pH 8.3 (borate/boric acid buffer, 100 mmol/L). Addition of detergent (Triton X-100-R, deoxycholate, and/or SDS) to the background electrolyte was required for reduction of wall adsorption and improvement of peak shape. A major nonviral contaminant, present in all virus samples, was best separated from the viral peaks with 10 mmol/L SDS as additive. The method allowed detecting serotypes HRV16 and HRV49 in crude, partially purified virus preparations. An infectivity assay carried out off-line with fractions collected at the capillary outlet enabled the sensitive and biospecific identification of the peaks of HRV2 and HRV14.
authors.affiliation: Institute of Analytical Chemistry, University of Vienna, Austria.
authors.forename: V
authors.initials: V
authors.lastname: Okun
authors.forename: B
authors.initials: B
authors.lastname: Ronacher
authors.forename: D
authors.initials: D
authors.lastname: Blaas
authors.forename: E
authors.initials: E
authors.lastname: Kenndler
mesh_terms: D019075:Electrophoresis, Capillary; D006367:HeLa Cells; D006801:Humans; D012229:Rhinovirus / Q000737:chemistry* / Q000145:classification / Q000472:pathogenicity*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013056:Spectrophotometry, Ultraviolet