title: Kinetic effects of the electrochemical proton gradient on plastoquinone reduction at the Qi site of the cytochrome b6f complex.
abstract: We have investigated the effects of the light-induced thylakoid transmembrane potential on the turnover of the b(6)f complex in cells of the unicellular green alga Chlamydomonas reinhardtii. The reduction of the potential by either decreasing the light intensity or by adding increasing concentrations of the ionophore carbonylcyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) revealed a marked inhibition of the cytochrome b(6) oxidation rate (10-fold) without substantial modifications of cytochrome f oxidation kinetics. Partial recovery of this inhibition could be obtained in the presence of ionophores provided that the membrane potential was re-established by illumination with a train of actinic flashes fired at a frequency higher than its decay. Measurements of isotopic effects on the kinetics of cytochrome b(6) oxidation revealed a synergy between the effects of ionophores and the H(2)O-D(2)O exchange. We propose therefore, that protonation events influence the kinetics of cytochrome b(6) oxidation at the Qi site and that these reactions are strongly influenced by the light-dependent generation of a transmembrane potential.
authors.affiliation: Centro di Studio del CNR sulla Biologia Cellulare e Molecolare delle Piante, via Celoria 26, 20133 Milano, Italy.
authors.forename: R P
authors.initials: RP
authors.lastname: Barbagallo
authors.forename: C
authors.initials: C
authors.lastname: Breyton
authors.forename: G
authors.initials: G
authors.lastname: Finazzi
mesh_terms: D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D016825:Chlamydomonas reinhardtii / Q000187:drug effects / Q000201:enzymology*; D000460:Chlorophyta; D003573:Cytochrome b Group / Q000037:antagonists & inhibitors / Q000378:metabolism*; D045346:Cytochrome b6f Complex; D003903:Deuterium; D004563:Electrochemistry; D007700:Kinetics; D008027:Light; D008564:Membrane Potentials / Q000187:drug effects; D010084:Oxidation-Reduction; D010971:Plastoquinone / Q000378:metabolism*; D011522:Protons; D014475:Uncoupling Agents / Q000494:pharmacology; D014867:Water
chemical_list: D003573:Cytochrome b Group; D011522:Protons; D014475:Uncoupling Agents; D014867:Water; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D045346:Cytochrome b6f Complex; D003903:Deuterium; D010971:Plastoquinone