title: Simultaneous separation of atovaquone, proguanil and its metabolites on a mixed mode high-performance liquid chromatographic column.
abstract: An isocratic high-performance liquid chromatographic (HPLC) method for simultaneous separation of the components in the antimalarial combination drug Malarone with UV detection is described. An HPLC system using a mixed mode column composed of 50% C(18) phase and 50% strong cation-exchanger has been optimised for the simultaneous separation of atovaquone, proguanil and its two main metabolites. The mobile phase was optimised for factors such as pH, counter ion concentration and acetonitrile. Elimination of interferences from other antimalarial drugs was achieved by adding sodium perchlorate to the mobile phase. With a mobile phase of acetonitrile-phosphate buffer (60:40, v/v) pH 6.8, 50.7 mmol l(-1) K+ and 10 mmol l(-1) Na x ClO4, separation was achieved within a run time shorter than 17 min.
authors.affiliation: Dalarna University College, Division of Clinical Pharmacology, Karolinska Institute, Huddinge University Hospital, Sweden. ybq@du.se
authors.forename: Y
authors.initials: Y
authors.lastname: Bergqvist
authors.forename: C
authors.initials: C
authors.lastname: Hopstadius
mesh_terms: D000097:Acetonitriles; D053626:Atovaquone; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D006863:Hydrogen-Ion Concentration; D009285:Naphthoquinones / Q000302:isolation & purification* / Q000378:metabolism; D002727:Proguanil / Q000302:isolation & purification* / Q000378:metabolism; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000097:Acetonitriles; D009285:Naphthoquinones; D002727:Proguanil; D053626:Atovaquone; C032159:acetonitrile