[The modulation of phagocyte oxygen metabolism by recombinant cytokines and a complex of natural cytokines].
The data on the modulating function of cytokines on the oxygen-producing function of peritoneal exudate cells of rats are presented. As priming agents, recombinant cytokines IL1 beta and TFR beta 1, as well as the natural complex of cytokines, were used. The priming action of cytokines was studied by changing in the production of active forms of oxygen by peritoneal exudate cells of rats, stimulated with opsonized zymosan, by the method of luminol-dependent chemiluminescence. The study revealed that IL1 beta and the natural complex of cytokines primed peritoneal exudate cells for the production of active forms of oxygen. The maximum value of the prestimulation index was 1.9 +/- 0.1 and 2.95 +/- 0.27 respectively. The preincubation of peritoneal exudate cells of rats with TFR beta 1 led to the pronounced inhibition of the intensity of the chemiluminescent response of cells. The prestimulation index did not exceed 1.06 +/- 0.1. Moreover, as revealed with the use of the probe Fura-2/AM, in the process the prestimulation of phagocytes with the natural complex of cytokines the intracellular concentration of calcium increased from 0.86 +/- 0.15 to 1.86 +/- 0.2 microM/ml. The mechanism of the prestimulation of peritoneal exudate cells of rats cytokines seems to be calcium-dependent.
Nikankina
L V
LV
State Medical University, Moscow, Russia.
Dolgina
E N
EN
Gankovskaia
L V
LV
Klebanov
G I
GI
Koval'chuk
L V
LV
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002118:Calcium / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology*; D004338:Drug Combinations; D007370:Interferon Type I / Q000494:pharmacology*; D008163:Luminescent Measurements; D010101:Oxygen Consumption / Q000187:drug effects* / Q000502:physiology; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D018709:Statistics, Nonparametric; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016207:Cytokines; D004338:Drug Combinations; D007370:Interferon Type I; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D002118:Calcium
Moduliatsiia kislorodnogo metabolizma fagotsitov rekombinantnymi tsitokinami i kompleksom prirodnykh tsitokinov.