Changes in circadian period and morphology of the hypothalamic suprachiasmatic nucleus in fyn kinase-deficient mice.
Protein tyrosine phosphorylation is involved in intracellular signal transduction and plays important roles in various physiological events. To understand the role of Fyn, a non-receptor type tyrosine kinase of Src family kinases, in the mechanism of circadian rhythms, we analyzed the circadian locomotor behavior and morphology of the hypothalamic suprachiasmatic nucleus (SCN), a master circadian oscillator in Fyn mutant mice, because Fyn-like immunoreactive substance was observed in the SCN. Under constant dark (DD) condition the Fyn (-/-) mutant mice showed a free-running circadian rhythm, and the period of the circadian rhythm of the locomotor activity was significantly longer than that of the control mice. Fyn (-/-) mutant mice had abnormal distribution of neurons containing vasoactive intestinal polypeptide (VIP)-like immunoreactive substance in the SCN. These findings suggest that Fyn is involved in the mechanism of circadian oscillation and morphological formation of the SCN. The mechanism of the implication of Fyn discussed with the Fyn's roles in neural network formation and cellular signal transduction pathway.
Shima
T
T
Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamada-Oka, Suita, 565-0871, Osaka, Japan.
Yagi
T
T
Isojima
Y
Y
Okumura
N
N
Okada
M
M
Nagai
K
K
D000818:Animals; D015153:Blotting, Western; D002940:Circadian Rhythm / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D009043:Motor Activity / Q000502:physiology; D009474:Neurons / Q000737:chemistry / Q000201:enzymology; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D051076:Proto-Oncogene Proteins c-fyn; D015398:Signal Transduction / Q000235:genetics; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000502:physiology*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D011518:Proto-Oncogene Proteins; D014660:Vasoactive Intestinal Peptide; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn