Interaction of phospholipid bilayers with polyamines of different length.
The tip-dip patch clamp method was used to study the effect of three polyamines, putrescine, spermidine and spermine, on bilayer lipid membrane (BLM) stability. Two kinds of mixed lipid-polyamine membranes were investigated. The poration voltage (V(p)), and the closed (sigma(cl)) and open (sigma(op)) state conductances for pure and polyamine-treated lipid membranes were determined by the method of current-voltage surfaces. It was demonstrated that putrescine and spermidine destabilized lipid membranes under all circumstances. BLM stabilization by spermine was observed when it was added to preformed membranes.
Zheliaskova
A
A
Biomolecular Layers Department, Institute of Solid State Physics, Bulgarian Academy of Sciences, Sofia. georgant@issp.bas.bg
Naydenova
S
S
Petrov
A G
AG
D004355:Drug Stability; D004563:Electrochemistry / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D008564:Membrane Potentials; D010713:Phosphatidylcholines / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D011700:Putrescine / Q000737:chemistry; D013095:Spermidine / Q000737:chemistry; D013096:Spermine / Q000737:chemistry; D013329:Structure-Activity Relationship
D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine