title: Functionalized nucleoside 5'-triphosphates for in vitro selection of new catalytic ribonucleic acids.
abstract: A series of novel 2'-modified nucleoside 5'-triphosphates was synthesized. The amino, imidazole, and carboxylate functionalities were attached to the 5-position of pyrimidine base of these molecules through alkynyl and alkyl spacers, respectively. Two different phosphorylation methods were used to optimize the yields of these highly modified triphosphates.
authors.affiliation: Department of Chemistry & Biochemistry, Ribozyme Pharmaceuticals, Inc., Boulder, CO 80301, USA.
authors.forename: J
authors.initials: J
authors.lastname: Matulic-Adamic
authors.forename: A T
authors.initials: AT
authors.lastname: Daniher
authors.forename: A
authors.initials: A
authors.lastname: Karpeisky
authors.forename: P
authors.initials: P
authors.lastname: Haeberli
authors.forename: D
authors.initials: D
authors.lastname: Sweedler
authors.forename: L
authors.initials: L
authors.lastname: Beigelman
mesh_terms: D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009711:Nucleotides; D016337:RNA, Catalytic