GC/MS/MS detection of pyrrolic metabolites in animals poisoned with the pyrrolizidine alkaloid riddelliine.
Pyrrolic metabolites from pyrrolizidine alkaloids (PAs) were detected in liver and dried blood samples using a gas chromatography/tandem mass spectrometry (GC/MS/MS) selected product-ion-monitoring method. A calibration curve was constructed using a protein-metabolite conjugate spiked into dried bovine blood. These spiked samples served as a model for tissues from animals poisoned by the toxic metabolite of PAs. Tissue samples from pigs fed various amounts of the PA alkaloid riddelliine (from Senecio riddellii) were analyzed for pyrrolic metabolites, and the results were applied to the calibration curve to provide a measure of the degree of PA poisoning. Pyrrolic metabolites were detected in liver and blood samples of all poisoned animals at levels between 2 and 64 ppm. Although differences in metabolite levels could be discerned under the reported experimental conditions, the amount detected did not correlate with the dose of riddelliine given; and livers fixed with formalin gave greatly reduced recovery than those same livers either frozen or freeze dried.
Schoch
T K
TK
United States Department of Agriculture, Agricultural Research Service, Poisonous Plant Research Laboratory, Logan, Utah 84341, USA.
Gardner
D R
DR
Stegelmeier
B L
BL
D000818:Animals; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008099:Liver / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008956:Models, Chemical; D010936:Plant Extracts / Q000737:chemistry; D011763:Pyrrolizidine Alkaloids / Q000009:adverse effects* / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013552:Swine
D010936:Plant Extracts; D011763:Pyrrolizidine Alkaloids; C013672:riddelliine; C068583:riddelliine N-oxide