Differentially expressed mRNAs in androgen-independent but not androgen-dependent Shionogi carcinoma.
Recently, a new and highly effective method termed suppressive subtractive hybridization (SSH) has been introduced to clone differentially expressed mRNAs. Genes expressed in androgen-independent but not in androgen-dependent tumors, and vice versa, are obviously significant to delineate the mechanisms of androgen dependency/independency of these tumors. Mouse mammary cancer (Shionogi carcinoma-115) has been extensively used to analyze the mechanism of androgen-dependent cancer growth.


METHODS
We cloned androgen-independent and androgen-dependent Shionogi carcinoma-115 specific mRNAs by the SSH method. Cloned sequences were compared with known sequences using NCBI BLAST across the Internet. Two clones were positive for cDNA insert when androgen-independent cDNA was used as tester cDNA, while no clones were positive using the androgen-dependent tester cDNA. One of the former was mouse protein kinase C beta-II while the other was a new DNA sequence. Mouse protein kinase C beta-II mRNA and the new mRNA were shown to be differentially expressed by RT-PCR analysis in androgen-independent but not androgen-dependent Shionogi carcinoma. Two mRNA species differentially expressed in androgen-independent but not androgen-dependent Shionogi carcinoma were cloned by the SSH method. The significance of these mRNAs for androgen-dependency/independency of Shionogi carcinoma should be explained in future studies.
Takeuchi
T
T
Department of Urology, Faculty of Medicine, University of Tokyo, Japan. TAKEUCHI-URO@h.u-tokyo.ac.jp
Nishimatsu
H
H
Ueki
T
T
Kajiwara
T
T
Fukuhara
H
H
Ishida
T
T
Moriyama
N
N
Kitamura
T
T
D000728:Androgens / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009376:Neoplasms, Hormone-Dependent / Q000235:genetics*; D015345:Oligonucleotide Probes; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis
D000728:Androgens; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D012333:RNA, Messenger