Evaluation of the cholera spot test: a chromatographic immunoassay for the rapid detection of cholera antigen.
A chromatographic immunoassay cholera antigen detection kit, the Cholera Spot test, was evaluated. The test was found to be specific with a sensitivity of 10(6) cfu/ml for the direct detection of V. cholerae in simulated stool specimens and 10 cfu/ml in simulated cotton-tipped swab specimens after overnight incubation in alkaline peptone water. This enables early recognition of cholera cases and their contacts so that prevention and control measures can be promptly instituted.
Rohani
M Y
MY
Bacteriology Division, Institute for Medical Research, Kuala Lumpur, Malaysia.
Hasnidah
D
D
Ong
K H
KH
D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis*; D002771:Cholera / Q000175:diagnosis* / Q000382:microbiology; D002845:Chromatography; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000276:immunology* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D011934:Reagent Strips