title: beta2-adrenoceptor gene polymorphisms and blood pressure variations in East Anglian Caucasians.
abstract: OBJECTIVE
  The amino-terminal polymorphisms, Arg16Gly and Gln27Glu, of the beta2-adrenergic receptor (beta2AR) have been shown to affect regulation of the receptor expression by an agonist in cell culture studies. The Arg16Gly polymorphism has also been recently shown to be associated with essential hypertension. We therefore evaluated whether the amino-terminal polymorphisms of beta2AR are associated with hypertension in a Caucasian population.
  
  
  METHODS
  We performed an association study in 298 hypertensive patients and an equal number of age-matched normotensive controls from the East Anglian region, with blood pressure assessed categorically and quantitatively. We also examined the influence of the amino-terminal polymorphisms on blood pressure response to beta-blockade in 144 of the patients randomly assigned to this class of drug. Genotyping of the Arg16Gly polymorphism was undertaken by a newly designed mismatched polymerase chain reaction (PCR) and digestion with Nde I, whereas the Gln27Glu polymorphism was genotyped by PCR followed by Fnu4H I cleavage.
  
  
  RESULTS
  We found no differences in the genotype or allele frequencies of the beta2AR polymorphisms between hypertensive and normotensive participants. There was also no association between the beta2AR genotypes and variations in either basal blood pressure or the blood pressure response to a beta-blocker.
  
  
  CONCLUSIONS
  These findings suggest that the amino-terminal polymorphisms of the beta2AR gene are unlikely to constitute major susceptibility for essential hypertension in the East Anglian population.
authors.affiliation: Clinical Pharmacology Unit, University of Cambridge, Addenbbrooke's Hospital, United Kingdom. haiyan.jia@ucl.ac.uk
authors.forename: H
authors.initials: H
authors.lastname: Jia
authors.forename: P
authors.initials: P
authors.lastname: Sharma
authors.forename: R
authors.initials: R
authors.lastname: Hopper
authors.forename: C
authors.initials: C
authors.lastname: Dickerson
authors.forename: D D
authors.initials: DD
authors.lastname: Lloyd
authors.forename: M J
authors.initials: MJ
authors.lastname: Brown
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000627:therapeutic use; D000483:Alleles; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D004739:England; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D016896:Treatment Outcome; D044465:White People / Q000235:genetics*
chemical_list: D000319:Adrenergic beta-Antagonists; D011943:Receptors, Adrenergic, beta