In vivo intracellular cytokine production by leukocytes during haemodialysis.
Several chronic inflammatory changes undergone during chronic haemodialysis are associated with increased pro-inflammatory cytokine production. Although generation of anaphylatoxins has been incriminated in the untoward effects of haemodialysis, it is still debated whether anaphylatoxins stimulate monocyte secretion of TNF-alpha and IL-1. We demonstrate that peripheral mononuclear cells isolated from healthy controls and cultured with complement-activated autologous serum or recombinant C5a induced high levels of IL-1, IL-1ra, IL-8 and MCP-1, low levels of TNFalpha and sTNFRII but no IL-10 and MIP-1alpha. Cytokine production by leukocytes was investigated by FACS analysis in six patients dialysed consecutively with three equivalent low permeability membranes known to activate the complement to different degrees: polysulfone (F6HPS), cellulose acetate (CA) and cuprophane (CP). Percentage of leukocytes expressing IL-1, IL-1ra, TNF-alpha and IL-8 is increased in patients dialysed with CP. Moreover, we show for the first time that haemodialysis is associated with the production of cytokines by circulating neutrophils. Predialysis plasma levels of MCP-1 and TNFRII did not increase during the dialysis session at the time when anaphylatoxin generation was highest. Dialysis with membranes that activate the complement to a high extent induce activation of leukocytes which may explain chronic complications associated with dialysing with CP.
Rousseau
Y
Y
Institut National de la Santé et de la Recherche Médicale, INSERM U430, Hôpital Broussais and Université Pierre et Marie Curie, Paris, France.
Haeffner-Cavaillon
N
N
Poignet
J L
JL
Meyrier
A
A
Carreno
M P
MP
D000328:Adult; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D008875:Middle Aged; D006435:Renal Dialysis
D016207:Cytokines