Measurement of loosely-bound iron in brain regions using redox cycling and salicylate.
A sensitive iron assay was developed for measuring non-heme and loosely bound iron in regions of rat brain. The method is based on the salicylate trapping of hydroxyl radicals generated from ascorbate-driven redox cycling of Fe3+-EDTA. This assay has high sensitivity (about 20 nM) because of amplification obtained with redox-cycling and fluorescent detection of the salicylate hydroxylation product, 2,5-dihydroxybenzoate. The assay detects iron as Fe2+ and Fe3+ combined. Values of non-heme and loosely bound iron are given for three areas of cortex, caudate, hippocampus, thalamus and brainstem of the rat brain.
Nelson
S R
SR
Ralph L. Smith Research Center, University of Kansas Medical Center, Kansas City 66160-7336, USA. snelson@iopener.net
Pazdernik
T L
TL
samson
F E
FE
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002138:Calibration; D002712:Chlorides; D005290:Ferric Compounds / Q000378:metabolism; D007501:Iron / Q000032:analysis*; D007502:Iron Chelating Agents / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D020156:Salicylic Acid / Q000378:metabolism*
D002712:Chlorides; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D007501:Iron; D020156:Salicylic Acid; C024555:ferric chloride