[Binding sites for A2 and A24 monoclonal antibodies on the alpha-latrotoxin molecule].
alpha-Latrotoxin (alpha-LTX) binding sites to functionally active monoclonal antibodies (MA) A4 and A24 were localized using three approaches: hydrolysis of the toxin followed by the N-terminal sequencing of immunoreactive peptides; the study of antibody interaction with several recombinant alpha-LTX fragments; and Western immunoblotting of synthetic overlapping peptides (6-8 aa) whose structures correspond to that of the immunoreactive alpha-LTX fragment. It was shown that the MA A4 epitope is located within the F234-M294 protein fragment and that of MA A24 interacts with the fragment 347FDKDIT352.
Lazareva
V D
VD
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Bocharova
N E
NE
Volynskiĭ
K E
KE
Volkova
T M
TM
Grishin
E V
EV
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001483:Base Sequence; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D017931:DNA Primers; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D013111:Spider Venoms / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D011994:Recombinant Proteins; D013111:Spider Venoms; C020763:alpha-latrotoxin
Uchastki uznavaniia monoklonal'nykh antitel A4 i A24 v molekule alpha-latrotoksina.