title: Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium.
abstract: Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16,525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21.
authors.affiliation: Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 10-Uhrineves, Prague, 104 00, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Fliegerova
authors.forename: S
authors.initials: S
authors.lastname: Pazoutova
authors.forename: P
authors.initials: P
authors.lastname: Pristas
authors.forename: H J
authors.initials: HJ
authors.lastname: Flint
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D020357:Selenomonas / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004269:DNA, Bacterial