Anti-type I allergic mechanisms of mao-bushi-saishin-to in mice.
We investigated the anti-allergic effect of mao-bushi-saishin-to (MBS) on the type I allergy model in mice. When MBS was administered orally at a dose of 0.5 or 1.0 g/kg, edema of the footpad, the amount of plasma IgE and the ratio of eosinophilic leukocytes in peritoneal exudate cells were all dose-relatedly suppressed. Moreover to investigate the anti-type I allergic mechanisms of MBS, enzyme-linked immunosorbent assay was performed to determine the interleukin (IL)-4, IL-5 and interferon (IFN)-gamma production from splenocytes that were stimulated by pokeweed mitogen for 48 h. In addition, we assayed IgE production from splenic B cells stimulated with the lipopolysaccharide and IL-4 for 7 days. MBS inhibited the IL-4 and IFN-gamma production, but IL-5 and IgE production were not affected. Thus possibly, the inhibition of IL-4 production may partially be involved in the expression of the anti-type I allergic effects of MBS.
Ikeda
Y
Y
Central Research Laboratories, Tsumura & Co., Ibaraki, Japan.
Iizuka
A
A
Amagaya
S
S
Ishige
A
A
Komatsu
Y
Y
Fujihira
E
E
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004487:Edema / Q000517:prevention & control; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology
D018926:Anti-Allergic Agents; D004365:Drugs, Chinese Herbal; C119422:mao-bushi-saishin-to; D015847:Interleukin-4; D007073:Immunoglobulin E