Progress in packed column supercritical fluid chromatography: materials and methods.
This article summarizes recent developments in packed column supercritical fluid chromatography. Silica-based chemically bonded sorbents, similar to those used for HPLC, are widely used with solvent-modified fluids containing additives to suppress undesirable solute-sorbent interactions that lead to poor peak shapes. Composition programming is the most useful approach to gradient elution separations since solvent-modified fluids have low compressibility. Packed column SFC is most useful for the separation of mixtures usually separated by normal-phase HPLC. Compared to normal-phase HPLC it offers faster separations, higher efficiencies, faster column re-equilibration, and a wider range of experimental variables for optimization. Packed column SFC is being increasingly selected for the analytical and preparative separation of racemic mixtures using enantiomer-selective sorbents.
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Wayne State University, Department of Chemistry, Rm. 171 Chemistry, Detroit, MI 48202, USA. cfp@chem.wayne.edu
D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006108:Graphite / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011108:Polymers / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012834:Silver / Q000737:chemistry
D011108:Polymers; D012834:Silver; D012822:Silicon Dioxide; D006108:Graphite