The role of the bacitracin ABC transporter in bacitracin resistance and collateral detergent sensitivity.
The bacitracin resistance of Bacillus licheniformis, a producer of bacitracin, is mediated by the ABC transporter Bcr. Bacillus subtilis cells carrying bcr genes on high-copy number plasmids developed collateral detergent sensitivity, as did human cells with overexpressed multidrug resistance P-glycoprotein. Resistance against bacitracin and sensitivity of resistant cells to detergents were shown to be inseparable phenomena associated with the membrane part of Bcr transporter, namely protein BcrC. A fused protein, consisting of ATP-binding protein BcrA and membrane component BcrC was constructed. It resembled a half molecule of P-glycoprotein and was capable of providing a significant degree of antibiotic resistance and detergent sensitivity.
Podlesek
Z
Z
Department of Biology, University of Ljubljana, Slovenia. zdravko.podlesek@uni-lj.si
Comino
A
A
Herzog-Velikonja
B
B
Grabnar
M
M
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D001407:Bacillus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D001414:Bacitracin / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014169:Transformation, Bacterial
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D003902:Detergents; D011993:Recombinant Fusion Proteins; C121796:bacitracin permease; D001414:Bacitracin