DNA in situ hybridization on polytene chromosomes of Simulium sanctipauli at loci relevant to insecticide resistance.
A DNA technique for in situ hybridization developed by Kumar & Collins (1994) for use on polytene chromosomes of adult Anopheles mosquitoes (Diptera: Culicidae) was modified for use with Simulium larval salivary gland chromosomes (Diptera: Simuliidae). Cloned fragments of several Simulium genes (coding for aspartate amino transferase, cytochrome P450 and DNA polymerase) were successfully mapped physically by assigning specific band locations in Simulim sanctipauli V. & D. This represents the first attempt at locating genes beyond the resolution of linkage to inversions in any blackfly species.
Boakye
D A
DA
Institute of Evolutionary and Ecological Sciences, Section of Evolutionary Biology, University of Leiden, The Netherlands. dboakye@noguchi.mimcom.net
Cornel
A J
AJ
Meredith
S E
SE
Brakefield
P M
PM
Collins
F H
FH
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D002875:Chromosomes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004351:Drug Resistance / Q000235:genetics; D017403:In Situ Hybridization / Q000662:veterinary*; D007306:Insecticides; D008969:Molecular Sequence Data; D012843:Simuliidae / Q000235:genetics*
D007306:Insecticides; D004247:DNA