title: Differential glycosylation of interleukin 2, the molecular basis for the NOD Idd3 type 1 diabetes gene?
abstract: The insulin-dependent diabetes (Idd) gene, Idd3, has been localised to a 0.35 cM region of chromosome 3 containing the structural gene for the cytokine interleukin 2 (IL-2). While variation of the N-terminal amino acid sequence of IL-2 has been shown to correlate with Idd3 allelic variation, differences in induction of proliferation by IL-2 allotypes have not been detected. In the current study, we examined the electrophoretic migration of IL-2 allotypes and have found two distinct patterns, consistent with differences in glycosylation, that correlate with diabetes-resistance and susceptibility. These findings strongly suggest that IL-2 variants may be functionally distinct.
authors.affiliation: Department of Autoimmune Diseases Research, Merck Research Laboratories, Rahway, New Jersey 07065, USA.
authors.forename: P L
authors.initials: PL
authors.lastname: Podolin
authors.forename: M B
authors.initials: MB
authors.lastname: Wilusz
authors.forename: R M
authors.initials: RM
authors.lastname: Cubbon
authors.forename: U
authors.initials: U
authors.lastname: Pajvani
authors.forename: C J
authors.initials: CJ
authors.lastname: Lord
authors.forename: J A
authors.initials: JA
authors.lastname: Todd
authors.forename: L B
authors.initials: LB
authors.lastname: Peterson
authors.forename: L S
authors.initials: LS
authors.lastname: Wicker
authors.forename: P A
authors.initials: PA
authors.lastname: Lyons
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D007376:Interleukin-2 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data
chemical_list: D007376:Interleukin-2