A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation.
A physical clone contig has been constructed, spanning 2 Mb on the proximal mouse X chromosome containing the mouse scurfy (sf) and tattered (Td) mutations. Extensive transcript mapping in this interval has identified 37 potential transcription units, including a number of novel genes, and 4 pseudogenes. These genes have been ordered by STS content and restriction mapping. Comparison of the transcript map to the corresponding region in human Xp11.23-p11.22 shows extensive homology, with complete conservation of gene order for loci in common between the two maps. Further, using a novel method to identify simple sequence length polymorphisms, we have developed a number of genetic markers, which has enabled the region containing the sf mutation to be narrowed to <300 kb. This contig has already allowed the cloning of the Td gene using a candidate gene approach and now serves as a starting point for the cloning of the sf mutation.
Means
G D
GD
Immunex Corporation, Seattle, Washington 98101-2936, USA.
Toy
D Y
DY
Baum
P R
PR
Derry
J M
JM
D000818:Animals; D002876:Chromosomes, Bacterial; D020451:Contig Mapping; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D006239:Haplotypes; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D014158:Transcription, Genetic; D014960:X Chromosome / Q000235:genetics*
D018076:DNA, Complementary