72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli.
An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.
von Wilcken-Bergmann
B
B
Institut für Genetik, Universität zu Köln, FRG.
Koenen
M
M
Griesser
H W
HW
Müller-Hill
B
B
D000595:Amino Acid Sequence; D019677:Artificial Gene Fusion; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic; D009876:Operon; D012097:Repressor Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C050993:Galactose repressor proteins; D012097:Repressor Proteins; D001616:beta-Galactosidase