title: Simultaneous determination of zidovudine and lamivudine in human serum using HPLC with tandem mass spectrometry.
abstract: A method employing high performance liquid chromatography (HPLC) with tandem mass spectrometry (MS) has been developed and validated for the simultaneous determination of clinically relevant levels of zidovudine (AZT) and lamivudine (3TC) in human serum. The method incorporates a fully automated ultrafiltration sample preparation step that replaces the solid-phase extraction step typically used for HPLC with UV detection. The calibration range of the dual-analyte LC-MS/MS method is 2.5-2,500 and 2.5-5,000 ng ml-1 for AZT and 3TC, respectively, using 0.25 ml of human serum. The lower limit of quantification was 2.5 ng ml-1 for each analyte, with a chromatographic run time of approximately 6 min. Overall accuracy, expressed as bias, and inter- and intra-assay precision are < +/- 7 and < 10% for AZT, and < +/- 5 and < 12.1% for 3TC over the full concentration ranges. A cross-validation study demonstrated that the LC-MS/MS method afforded equivalent results to established methods consisting of a radioimmuno-assay for AZT and an HPLC-UV method for 3TC. Moreover, the LC-MS/MS was more sensitive, allowed markedly higher-throughput, and required smaller sample volumes (for 3TC only). The validated method has been used to support post-marketing clinical studies for Combivir a combination tablet containing AZT and 3TC.
authors.affiliation: Division of Bioanalysis and Drug Metabolism, Glaxo Wellcome Research Inc, Research Triangle Park, NC 27709, USA.
authors.forename: K B
authors.initials: KB
authors.lastname: Kenney
authors.forename: S A
authors.initials: SA
authors.lastname: Wring
authors.forename: R M
authors.initials: RM
authors.lastname: Carr
authors.forename: G N
authors.initials: GN
authors.lastname: Wells
authors.forename: J A
authors.initials: JA
authors.lastname: Dunn
mesh_terms: D019380:Anti-HIV Agents / Q000097:blood*; D000918:Antibody Specificity; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007553:Isotope Labeling; D019259:Lamivudine / Q000097:blood*; D013058:Mass Spectrometry; D011863:Radioimmunoassay; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D015215:Zidovudine / Q000097:blood*
chemical_list: D019380:Anti-HIV Agents; D007202:Indicators and Reagents; D019259:Lamivudine; D015215:Zidovudine