Bacterial, viral and parasitic enteric pathogens associated with acute diarrhea in hospitalized children from northern Jordan.
To determine the etiology of acute diarrhea in Jordanian children under 5 years of age, we examined stool samples from 265 children admitted to the pediatric ward at Princess Rahma Hospital for Children, Irbid, Jordan, for parasites, rotavirus and enteric bacteria. Using both traditional and molecular diagnostic techniques, we detected enteropathogens in 66.4% of patients with diarrhea. A single enteric pathogen was detected in 50.9% of the children, and multiple pathogens were detected in 15.5%. The prevalence of enteropathogens identified was as follows: rotavirus (32.5%), enteropathogenic Escherichia coli (12.8%), enteroaggregative E. coli (10.2), enterotoxigenic E. coli (5.7%), Shigella spp. (4.9%), Entamoeba histolytica (4.9%), Salmonella spp. (4.5%), Campylobacter jejuni/coli (1.5%), Cryptosporidium spp. (1.5%), enteroinvasive E. coli (1.5%), eae-, Ehly-positive E. coli (0.8%), Giardia lamblia (0. 8%) and Yersinia enterocolitica (0.4%). No Vibrio cholerae, Shiga toxin-producing E. coli, microsporidia, adenovirus or small round virus were detected. Findings from this study demonstrate that rotavirus and several types of diarrheagenic E. coli, which are not screened for during routine examinations of stool samples in public health laboratories, were the most frequently detected enteropathogens in these children. Our findings highlight the value of using a combination of traditional and molecular techniques in the diagnosis of diarrheal disease in this population.
Youssef
M
M
Department of Biological Sciences, Yarmouk University, Irbid, Jordan. mcy6@cdc.gov
Shurman
A
A
Bougnoux
M
M
Rawashdeh
M
M
Bretagne
S
S
Strockbine
N
N
D000208:Acute Disease; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D002675:Child, Preschool; D017931:DNA Primers; D003967:Diarrhea / Q000453:epidemiology / Q000209:etiology* / Q000473:pathology; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D056890:Eukaryota / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology / Q000469:parasitology; D005260:Female; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007597:Jordan / Q000453:epidemiology; D008297:Male; D015995:Prevalence; D012401:Rotavirus / Q000302:isolation & purification*
D017931:DNA Primers