Regulation of CD69 expression on eosinophil precursors by interferon-gamma.
The purpose of this study was to determine whether interferon-gamma (IFN-gamma) induced CD69 expression by eosinophil precursors. Eosinophil precursors were induced from CD34+ cord blood cells using recombinant human interleukin-3 (IL-3) and interleukin-5 (IL-5). On day 14 of culture, cells constitutively expressed CD69 and the IFN-gamma receptor (IFN-gammaR). Stimulation with IFN-gamma for 24 h did not affect IFN-gammaR expression by the cells. On the other hand, IFN-gamma significantly upregulated CD69 expression by the precursors after 24 h of incubation. A specific JAK2 inhibitor (AG-490) caused a concentration-dependent suppression of IFN-gamma-induced CD69 expression by the precursors. In conclusion, these results indicate that IFN-gamma induces CD69 expression by eosinophil precursors via the activation of JAK2.
Ochiai
K
K
Department of Internal Medicine, Toho University School of Medicine, Sakura Hospital, Sakura, Japan.
Kagami
M
M
Nakazawa
T
T
Sugiyama
T
T
Sueishi
M
M
Ito
M
M
Tomioka
H
H
D015703:Antigens, CD / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D004804:Eosinophils / Q000737:chemistry / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D053614:Janus Kinase 2; D037181:Lectins, C-Type; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins; D017471:Receptors, Interferon / Q000032:analysis; D020032:Tyrphostins / Q000494:pharmacology; D000096743:Interferon gamma Receptor; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007371:Interferon-gamma; D053614:Janus Kinase 2; D037181:Lectins, C-Type; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D017471:Receptors, Interferon; D020032:Tyrphostins; D000096743:Interferon gamma Receptor; D000099192:CD69 Antigens; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; C507924:JAK2 protein, human