A novel and single step ultrastructural method for staining biological tissues embedded in common embedding media.
A new single step staining method is described which is very simple, rapid and reproducible with better contrast than the widely employed uranyl acetate-lead citrate double staining method for observation of cell organelles in various tissue sections using transmission electron microscope. A copper uranyl acetate solution was employed to stain the ultrathin sections of tissues embedded in either water-miscible or water-immiscible resin media. The staining results are excellent. Cell organelles and extracellular matrix are stained with no artifactual precipitation or granulation.
Lokanadam
B
B
Department of Biophysics and Electron Microscopy, Central Leather Research Institute, Adyar, Chennai, India.
Gupta
P D
PD
Ramasami
T
T
D000818:Animals; D001808:Blood Vessels / Q000648:ultrastructure; D004396:Coloring Agents; D007422:Intestines / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D010870:Pineal Gland / Q000648:ultrastructure; D051381:Rats; D016707:Tissue Fixation / Q000379:methods*
D004396:Coloring Agents