title: Noninvasive assessment and necropsy validation of changes in left ventricular mass in ascending aortic banded mice.
abstract: Although left ventricular (LV) hypertrophy can be induced by aortic banding, noninvasive assessment of changes in LV mass in mice with a banded ascending aorta by using 2-dimensional (2D) images has not been previously performed. In this study we serially assessed changes in LV mass by 2D echocardiography with a newly available 12-MHz transducer in mice with a banded ascending aorta and validated measurements at necropsy. Estimated by echocardiography, LV mass increased from 74+/- 17 mg before banding to 191.08+/-54 mg at 8 weeks after banding (P <.0001), and excellent correlation was shown with postmortem measurements (r = 0.97). Furthermore, with the use of pulsed Doppler 2-dimensionally guided echocardiography, noninvasive measurement of flow velocities in the ascending aorta before and after the band at the various time points was possible. We propose that 2D echocardiography with a 12-MHz transducer is a powerful tool for serial noninvasive evaluations as an adjunct to the study of cardiac hypertrophy in the murine model.
authors.affiliation: Division of Cardiology, Department of Medicine, Columbia University, New York, NY 10032, USA.
authors.forename: A
authors.initials: A
authors.lastname: Fard
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Takuma
authors.forename: H A
authors.initials: HA
authors.lastname: Skopicki
authors.forename: D J
authors.initials: DJ
authors.lastname: Pinsky
authors.forename: M R
authors.initials: MR
authors.lastname: Di Tullio
authors.forename: S
authors.initials: S
authors.lastname: Homma
mesh_terms: D000818:Animals; D001011:Aorta / Q000503:physiopathology; D004195:Disease Models, Animal; D018619:Echocardiography, Doppler, Pulsed; D005260:Female; D017379:Hypertrophy, Left Ventricular / Q000000981:diagnostic imaging*; D051379:Mice; D008807:Mice, Inbred BALB C; D012039:Regional Blood Flow