BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3'.
The recognition sequence and cleavage positions of a new restriction endonuclease BTR:I isolated from Bacillus stearothermophilus SE-U62 have been determined. BTR:I belongs to a rare type IIQ of restriction endonucleases, which recognise non-palindromic nucleotide sequences and cleave DNA symmetrically within them.
Degtyarev
S K
SK
SibEnzyme, 9 Ak.Timakov Str., Novosibirsk 630117, Russia.
Belichenko
O A
OA
Lebedeva
N A
NA
Dedkov
V S
VS
Abdurashitov
M A
MA
D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D013379:Substrate Specificity
D004279:DNA, Viral; C410662:endodeoxyribonuclease BtrI; D015252:Deoxyribonucleases, Type II Site-Specific