title: The immune response of mice and cynomolgus monkeys to macaque mucin 1-mannan.
abstract: Mice immunised with human epithelial mucin MUC1 coupled to oxidised mannan produce MUC1 specific MHC Class 1 restricted CD8(+) cytotoxic T cells and are completely protected from the development of MUC1(+) tumours; such therapy may be applicable to humans. In this light we describe pre-clinical studies in cynomolgus monkeys (Macaca fascicularis), to test the efficacy of mannan-MUC1 in higher primates. Monkey MUC1 genomic clones were isolated from a macaque library, peptides and fusion protein synthesised and mice and monkeys immunised with macaque MUC1-mannan. In mice CTL responses were induced (as has been found with human MUC1 mannan conjugates), but in contrast monkeys produced a humoral response, with no T cell proliferative, cytotoxic responses or CTLp found. In spite of the presence of anti-MUC1 auto-antibodies, there was no toxicity or induction of autoimmunity.
authors.affiliation: The Austin Research Institute, A&RMC, StudleyRoad, Vic 3084, Heidelberg, Australia.
authors.forename: H A
authors.initials: HA
authors.lastname: Vaughan
authors.forename: D W
authors.initials: DW
authors.lastname: Ho
authors.forename: V
authors.initials: V
authors.lastname: Karanikas
authors.forename: M S
authors.initials: MS
authors.lastname: Sandrin
authors.forename: I F
authors.initials: IF
authors.lastname: McKenzie
authors.forename: G A
authors.initials: GA
authors.lastname: Pietersz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003429:Cross Reactions; D005091:Exons; D007114:Immunization; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008351:Mannans / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011110:Polymorphism, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D020080:Tandem Repeat Sequences
chemical_list: D008351:Mannans; D018396:Mucin-1