title: New fluorogenic substrate for the first continuous steroid sulfatase assay.
abstract: The screening for new inhibitors of steroid sulfatase requires an efficient test system. To overcome the shortcomings of the available discontinuous fluorimetric assay, several coumarin-type compounds were investigated as potential new substrates. 3,4-Benzocoumarin 7-O-sulfate was found to have appropriate substrate properties for the establishment of the first direct continuous assay of steroid sulfatase.
authors.affiliation: NOVARTIS Research Institute, Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Bilban
authors.forename: A
authors.initials: A
authors.lastname: Billich
authors.forename: M
authors.initials: M
authors.lastname: Auer
authors.forename: P
authors.initials: P
authors.lastname: Nussbaumer
mesh_terms: D001192:Arylsulfatases / Q000037:antagonists & inhibitors*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003374:Coumarins / Q000138:chemical synthesis* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology*; D005470:Fluorometry; D006801:Humans; D007700:Kinetics; D011994:Recombinant Proteins; D043266:Steryl-Sulfatase; D013329:Structure-Activity Relationship; D013429:Sulfatases / Q000037:antagonists & inhibitors
chemical_list: C414669:3,4-benzocoumarin 7-O-sulfate; D003374:Coumarins; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D013429:Sulfatases; C020121:estrone sulfatase; D001192:Arylsulfatases; D043266:Steryl-Sulfatase