title: [Introduction of rabbit defensin NP-1 gene into poplar (P. tomentosa) by Agrobacterium-mediated transformation].
abstract: Rabbit defensin NP-1 possesses a broad resistant spectrum to pathogens. In this work, we have transferred the NP-1 gene into poplar plants by Agrobacterium-mediated transformation. PCR amplification and Southern analysis showed that rabbit defensin NP-1 gene was integrated into the poplar genome. The transformation efficiency is about 15.6%. Antimicrobial activity test showed that the extract of transgenic plants inhibited the growth of the tested microbes.
authors.affiliation: Institute of Genetics, Chines Academy of Sciences, Beijing.
authors.forename: S M
authors.initials: SM
authors.lastname: Zhao
authors.forename: G C
authors.initials: GC
authors.lastname: Zu
authors.forename: G Q
authors.initials: GQ
authors.lastname: Liu
authors.forename: M R
authors.initials: MR
authors.lastname: Huang
authors.forename: J X
authors.initials: JX
authors.lastname: Xu
authors.forename: Y R
authors.initials: YR
authors.lastname: Sun
mesh_terms: D000818:Animals; D023082:Defensins; D018014:Gene Transfer Techniques; D030821:Plants, Genetically Modified; D011506:Proteins / Q000235:genetics*; D011817:Rabbits; D012231:Rhizobium / Q000235:genetics*
chemical_list: D023082:Defensins; D011506:Proteins