Functional genomics with protein-protein interactions.
Knowing the sequence of a gene does not mean knowing its function. Although, information stored at the DNA level can be used to predict biological processes, proteins are the final executors of the various response programs of a cell. Transient information, like posttranslational modifications or interactions among proteins, cannot be deduced from DNA sequences. The rapid accumulation of large amounts of DNA sequence data in genomics projects has led to an increasing demand for powerful tools to analyze proteins and their behaviour at a large scale. This review aims to compare different technologies used for identification of interacting proteins and discusses recent developments in the field of high-throughput protein-protein interaction mapping.
Rudert
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Xerion Pharmaceuticals GmbH, Martinsried/Munich, Germany. f.rudert@xerion-pharma.com
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D001435:Bacteriophages / Q000235:genetics; D005057:Eukaryotic Cells; D005544:Forecasting; D005821:Genetic Techniques; D008024:Ligands; D013058:Mass Spectrometry / Q000379:methods; D008967:Molecular Biology / Q000379:methods*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D008024:Ligands; D011506:Proteins