A panel of CAb antibodies recognize endogenous and ectopically expressed ING1 protein.
Nine monoclonal antibodies (MAbs) against human and rodent ING1 protein have been generated using an IL6-secreting mouse myeloma line. These antibodies are all effective in recognizing ING1 protein in ELISAs, Western blot assays, and by indirect immunofluorescence. Combining different CAb monoclonal antibodies in a Western blot assay also allows detection of the very low levels of endogenous ING1 found in fibroblast cells in culture and the identification of at least two isoforms of ING1 in normal human diploid fibroblasts and established brain cancer cell lines.
Boland
D
D
S.A.C.R.C. Hybridoma Facility, Southern Alberta Cancer Research Centre, The University of Calgary, Alberta, Canada.
Olineck
V
V
Bonnefin
P
P
Vieyra
D
D
Parr
E
E
Riabowol
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology; D019084:Fluorescent Antibody Technique, Indirect; D016147:Genes, Tumor Suppressor; D006801:Humans; D007132:Immunoglobulin Isotypes; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins; D020033:Protein Isoforms / Q000276:immunology; D011506:Proteins / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C501503:ING1 protein, human; D007132:Immunoglobulin Isotypes; C501505:Ing1 protein, mouse; D000076023:Inhibitor of Growth Protein 1; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D011506:Proteins; D025521:Tumor Suppressor Proteins