title: Chiral discrimination of N-carbazole-carbonyl derivatives of alpha-amino acids with a short linear alkyl side chain by bovine serum albumin.
abstract: Chiral discrimination of racemic carbazole carbonyl (CC)-amino acids with linear alkyl sidechain (C(1)-C(4)) by bovine serum albumin (BSA) was investigated by competitive replacement experiments using dansyl-L-proline and dansyl-D-norvaline as fluorescent probes. It was found that the CC derivatives of the D-forms of alanine (C(1)), amino butyric acid (C(2)), norvaline (C(3)), and norleucine (C(4)) bound to the dansyl-L-proline site much more strongly than their L-forms, whereas the interactions between both enantiomers of these amino acids with dansyl-D-norvaline site were slight.
authors.affiliation: Kyoritsu College of Pharmacy, Tokyo, Japan. abe-ys@kyoritsu-ph.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.forename: T
authors.initials: T
authors.lastname: Shoji
authors.forename: M
authors.initials: M
authors.lastname: Matsubara
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Sugata
authors.forename: K
authors.initials: K
authors.lastname: Iwata
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000409:Alanine / Q000737:chemistry; D000478:Alkylation; D000596:Amino Acids / Q000737:chemistry*; D001665:Binding Sites; D002227:Carbazoles / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014633:Valine / Q000737:chemistry
chemical_list: D000596:Amino Acids; D002227:Carbazoles; D003619:Dansyl Compounds; D012710:Serum Albumin, Bovine; D011392:Proline; D014633:Valine; D000409:Alanine