title: Expulsion of allergen-containing materials from hydrated rye grass (Lolium perenne) pollen revealed by using immunogold field emission scanning and transmission electron microscopy.
abstract: BACKGROUND
  Several studies demonstrated episodes of grass pollen-induced allergic asthma after heavy rainfalls. It has been hypothesized that these asthma attacks might be due to the release of respirable allergen-bearing particles from pollen cytoplasm.
  
  
  OBJECTIVE
  In this study we investigated the release mechanism of the most potent and frequently recognized grass pollen allergens, group 1 and group 5, from freshly harvested and subsequently hydrated rye grass pollen at the ultrastructural level.
  
  
  METHODS
  Rabbit antisera against purified recombinant group 1 and group 5 allergens were used to investigate, by using field emission scanning and transmission immunogold electron microscopy, the allergen release from rye grass pollen grains into isotonic aqueous solutions or water.
  
  
  RESULTS
  Pollen grains exposed to isotonic aqueous solutions remained intact and released allergens by means of diffusion. However, pollen grains hydrated in distilled water or rainwater expelled starch grains and cytoplasmic debris of respirable size. Group 1 and group 5 allergens were observed on and within these materials.
  
  
  CONCLUSIONS
  Exposure of rye grass pollen to water leads to an expulsion of subcellular allergen-containing pollen components of respirable size. Our ultrastructural data thus support the idea that this release of allergen-containing respirable pollen materials may be a cause of asthma attacks after heavy rainfalls.
authors.affiliation: Institute of Medical Physics and Biophysics, University of Münster; Germany.
authors.forename: M
authors.initials: M
authors.lastname: Grote
authors.forename: S
authors.initials: S
authors.lastname: Vrtala
authors.forename: V
authors.initials: V
authors.lastname: Niederberger
authors.forename: R
authors.initials: R
authors.lastname: Valenta
authors.forename: R
authors.initials: R
authors.lastname: Reichelt
mesh_terms: D000485:Allergens / Q000276:immunology*; D003593:Cytoplasm / Q000648:ultrastructure; D007150:Immunohistochemistry; D008129:Lolium / Q000276:immunology / Q000648:ultrastructure; D008854:Microscopy, Electron; D011058:Pollen / Q000276:immunology*
chemical_list: D000485:Allergens