title: Comparative study of epithelial and fibroblastic cell lines as an alternative cytotoxicity test to the Draize method.
abstract: Several methods are being used with considerable advantage as alternatives to the Draize test, although some technical difficulties still persist. This work compared the sensitivity of HeLa and NCTC L 929 cells to evaluate the cytotoxicity of shampoos used by adults and children (undiluted and diluted to 25, 5, 1, and 0.1%), and eye drops and their containers and surfactants (diluted to 30, 10, 1, and 0.1%). Nondiluted adult shampoos and their 25 and 5% dilutions were cytotoxic for both cell lines. When diluted to 1%, only one of the shampoos was noncytotoxic, whereas among those diluted to 0.1%, only one was cytotoxic. Children's shampoos were cytotoxic when not diluted or diluted to 25%. From those diluted to 5%, only one was noncytotoxic for both cell lines. The cytotoxic tests showed that the eye drops and their containers were noncytotoxic. Surfactants were cytotoxic when diluted to 30 and 10% and noncytotoxic when diluted to 1 and 0.1%. An excellent correlation (r = 0.95) was demonstrated between the sensitivity of the HeLa and NCTC L929 cells in the evaluation of cytotoxicity reactions.
authors.affiliation: Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Farmácia, Brazil.
authors.forename: T J
authors.initials: TJ
authors.lastname: Pinto
authors.forename: J C
authors.initials: JC
authors.lastname: de Azevedo
authors.forename: A S
authors.initials: AS
authors.lastname: Cruz
mesh_terms: D000328:Adult; D002460:Cell Line; D002648:Child; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004847:Epithelial Cells / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects*; D006203:Hair Preparations / Q000009:adverse effects; D006367:HeLa Cells; D006801:Humans
chemical_list: D006203:Hair Preparations