Adenylate kinase amplification of ATP bioluminescence for hygiene monitoring in the food and beverage industry.
Fourteen food residues, Escherichia coli O157:H7 and Staphylococcus aureus on stainless steel surfaces were detected using a combined assay with adenylate kinase as a cellular marker and ATP bioluminescence. The limit of sensitivity ranged from 0.02 to 708 microg for minced meat and broccoli, respectively. Both methods gave the same detection limit (105 cfu) for E. coli and Staph. aureus on stainless steel surfaces. The combined adenylate kinase-ATP assay is applicable to monitor the hygiene of work surfaces, especially those prone to contamination by meat and vegetable residues.
Corbitt
A J
AJ
Department of Life Sciences, The Nottingham Trent University, Nottingham and Biotrace Ltd, The Science Park, Bridgend, UK.
Bennion
N
N
Forsythe
S J
SJ
D000255:Adenosine Triphosphate / Q000032:analysis*; D000263:Adenylate Kinase / Q000032:analysis*; D001628:Beverages / Q000032:analysis; D003611:Dairy Products / Q000032:analysis; D019453:Escherichia coli O157 / Q000302:isolation & purification; D019649:Food Industry / Q000592:standards*; D005516:Food Microbiology; D006920:Hygiene / Q000592:standards*; D008163:Luminescent Measurements; D008460:Meat / Q000032:analysis; D011202:Poultry Products / Q000032:analysis; D013211:Staphylococcus aureus / Q000302:isolation & purification
D000255:Adenosine Triphosphate; D000263:Adenylate Kinase