title: Active zones on motor nerve terminals contain alpha 3beta 1 integrin.
abstract: Active zones are the sites along nerve terminals where synaptic vesicles dock and undergo calcium-dependent exocytosis during synaptic transmission. Here we show, by immunofluorescent staining with antibodies generated against Xenopus laevis integrins, that alpha3beta1 integrin is concentrated at the active zones of Xenopus motor nerve terminals. Because integrins can link extracellular matrix molecules to cytoskeletal elements and participate in the formation of signaling complexes, the localization of integrin at active zones suggests that it may play a role in the adhesion of the nerve terminals to the synaptic basal lamina, in the formation and maintenance of active zones, and in some of the events associated with calcium-dependent exocytosis of neurotransmitter. Our findings also indicate that the integrin composition of the terminal Schwann cells differs from that of the motor nerve terminals, and this may account at least in part for differences in their adhesiveness to the synaptic basal lamina.
authors.affiliation: Department of Physiology, McGill University, Montreal, Quebec Canada H3G 1Y6. monroe@med.mcgill.ca
authors.forename: M W
authors.initials: MW
authors.lastname: Cohen
authors.forename: B G
authors.initials: BG
authors.lastname: Hoffstrom
authors.forename: D W
authors.initials: DW
authors.lastname: DeSimone
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion; D017364:Collagenases; D039201:Integrin alpha3beta1; D016023:Integrins / Q000032:analysis*; D009046:Motor Neurons / Q000166:cytology* / Q000648:ultrastructure; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D018482:Muscle, Skeletal / Q000294:innervation; D009411:Nerve Endings / Q000648:ultrastructure*; D009469:Neuromuscular Junction / Q000166:cytology* / Q000648:ultrastructure; D012583:Schwann Cells / Q000166:cytology / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure; D014982:Xenopus laevis
chemical_list: D000911:Antibodies, Monoclonal; D039201:Integrin alpha3beta1; D016023:Integrins; D017364:Collagenases