Supercritical carbon dioxide extraction of lipids from Eucalyptus globulus wood.
Various typical lipid components of wood extractives have been isolated from Eucalyptus globulus wood by supercritical carbon dioxide modified with methanol. The influence of various extraction parameters on the yield and qualitative composition of the extracts have been studied. The extracts were analyzed by gas chromatography-mass spectrometry and compared with those obtained by Soxhlet extraction with acetone, the standard method for the determination of wood extractives. The qualitative and quantitative results obtained by both methods were in good agreement. The experimental planning to asses the influence of pressure, temperature and percentage of methanol and their interactions on the extraction efficiency was carried out with a factorial design, followed by multiple linear regression algorithm.
González-Vila
F J
FJ
Instituto de Recursos Naturales y Agrobiología, CSIC, P.O. Box 1052, E-41080, Sevilla, Spain. fjgon@irnase.csic.es
Bautista
J M
JM
Gutiérrez
A
A
Del Rio
J C
JC
González
A G
AG
D002245:Carbon Dioxide / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D002849:Chromatography, Gas; D005052:Eucalyptus / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D016014:Linear Models; D008055:Lipids / Q000302:isolation & purification*; D015233:Models, Statistical; D010946:Plants, Medicinal; D013997:Time Factors; D014934:Wood
D008055:Lipids; D002245:Carbon Dioxide