The mouse Psma1 gene coding for the alpha-type C2 proteasome subunit: structural and functional analysis, mapping, and colocalization with Pde3b on mouse chromosome 7.
We have isolated and functionally characterized the mouse gene for the C2 subunit of the 20S proteasome. The gene contains 10 exons distributed over a region of 12 kb on the distal end of mouse chromosome 7. Its exon-intron structure differs from those of the other few known proteasome genes. Transfection assays revealed that 1.5 kb of 5' flanking sequence is active as promoter in cultured myoblasts. Deletion reporter constructs narrowed this presumptive promoter region to within 450 bp upstream of the translation initiation site. Several consensus motifs for transcription factor binding sites were identified in this upstream region of the gene. Psma1 was mapped to mouse chromosome 7 using the interspecific backcross DNA panels from The Jackson Laboratory. Additional mapping studies showed that the mouse genes Psma1 and Pde3b are closely linked, residing between cM 53 and 53.3 in a region syntenic to human chromosome 11p15. Our results extend the structural and functional analysis of genes encoding the 20S proteasome subunits and provide the basis for the study of their regulation.
Hopitzan
A
A
Research and Development, Boehringer Ingelheim Austria, Vienna, 1121, Austria.
Himmelbauer
H
H
Spevak
W
W
Castanon
M J
MJ
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000235:genetics*; D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D003546:Cysteine Endopeptidases / Q000235:genetics*; D017930:Genes, Reporter; D007178:Inbreeding; D051379:Mice; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D020161:Physical Chromosome Mapping; D011401:Promoter Regions, Genetic / Q000235:genetics; D046988:Proteasome Endopeptidase Complex; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D020121:5' Untranslated Regions; D009097:Multienzyme Complexes; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; C517291:PDE3B protein, human; C517293:Pde3b protein, mouse; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex