title: [Binding sites for A2 and A24 monoclonal antibodies on the alpha-latrotoxin molecule].
abstract: alpha-Latrotoxin (alpha-LTX) binding sites to functionally active monoclonal antibodies (MA) A4 and A24 were localized using three approaches: hydrolysis of the toxin followed by the N-terminal sequencing of immunoreactive peptides; the study of antibody interaction with several recombinant alpha-LTX fragments; and Western immunoblotting of synthetic overlapping peptides (6-8 aa) whose structures correspond to that of the immunoreactive alpha-LTX fragment. It was shown that the MA A4 epitope is located within the F234-M294 protein fragment and that of MA A24 interacts with the fragment 347FDKDIT352.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: V D
authors.initials: VD
authors.lastname: Lazareva
authors.forename: N E
authors.initials: NE
authors.lastname: Bocharova
authors.forename: K E
authors.initials: KE
authors.lastname: Volynskiĭ
authors.forename: T M
authors.initials: TM
authors.lastname: Volkova
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001483:Base Sequence; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D017931:DNA Primers; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D013111:Spider Venoms / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers; D011994:Recombinant Proteins; D013111:Spider Venoms; C020763:alpha-latrotoxin
vernacular_title: Uchastki uznavaniia monoklonal'nykh antitel A4 i A24 v molekule alpha-latrotoksina.