title: [Isolation of toxigenic strains of Clostridium difficile and enterotoxin producing Bacteroides fragilis from fecal specimens of patients suspected of antibiotic associated diarrhoea].
abstract: Fifty faecal samples from patients suspected of AAD (antibiotic associated diarrhoea) were studied for Clostridium difficile and enterotoxin producing Bacteroides fragilis (ETBF). Using TCD (Becton-Dickinson) and C. difficile Toxin A test (Oxoid) in 34% of specimens the presence of toxin A was detected. From all specimens 25 C. difficile strains were isolated. All isolated strains produced toxin B in vitro which was shown in Mc Coy cytotoxicity test. Eighteen strains only were toxin A positive in vitro. From all isolated C. difficile strains 28% were tox A (-) tox B (+). By means of PCR presence of toxin A and toxin B genes was tested directly in faecal samples and in strains. From the same 50 faecal samples 17 B. fragilis strains were isolated. Four of them produced the enterotoxin (fragilisin) which was detected on the HT 29/C1 cell line. Genes of fragilisin were found in strains and directly in faecal samples. Toxin producing C. difficile and B. fragilis (ETBF) together were found in 3 samples. From one faecal sample only ETBF was cultured.
authors.affiliation: Zakład Mikrobiologii Lekarskiej AM w Warszawie.
authors.forename: H
authors.initials: H
authors.lastname: Pituch
authors.forename: P
authors.initials: P
authors.lastname: Leszczyński
authors.forename: A
authors.initials: A
authors.lastname: van Belkum
authors.forename: P
authors.initials: P
authors.lastname: Obuch-Woszczatyński
authors.forename: G S
authors.initials: GS
authors.lastname: Rouyan
authors.forename: F
authors.initials: F
authors.lastname: Meisel-Mikołajczyk
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D001441:Bacteroides fragilis / Q000302:isolation & purification* / Q000378:metabolism; D002648:Child; D016360:Clostridioides difficile / Q000302:isolation & purification* / Q000378:metabolism; D003967:Diarrhea / Q000139:chemically induced* / Q000382:microbiology*; D004768:Enterotoxins / Q000096:biosynthesis*; D005243:Feces / Q000382:microbiology*; D006801:Humans
chemical_list: D000900:Anti-Bacterial Agents; D004768:Enterotoxins
vernacular_title: Izolacja toksynotwórczych szczepów Clostridium difficile i enterotoksynotwórczych szczepów Bacteroides fragilis z kału osób z podejrzeniem biegunki poantybiotykowej.