Leukocyte adhesiveness/aggregation test (LAAT) to discriminate between viral and bacterial infections in children.
OBJECTIVE
We previously noted that white blood cells (WBC) have increased adhesive properties during bacterial infections. Here, we aim to explore the possibility of using the different adhesive properties of WBC as a means of differentiating between viral and bacterial infections, a common problem in paediatrics.


METHODS
The adhesive properties of WBC in the peripheral blood of 25 children with documented bacterial infections, 15 with documented viral infections and 36 with probable viral infections, were studied by means of a leukocyte adhesiveness/aggregation slide test (LAAT). The results of the LAAT were compared with those of the other acute phase reactants, namely WBC, differential count and erythrocyte sedimentation rate (ESR), which were taken in the same blood sample in each patient.


RESULTS
The sensitivity, specificity and positive predictive value were 92%, 96%, and 92%, respectively for the LAAT; 83%, 87% and 80% for the ESR; 56%, 78% and 56% for the white blood cell count; and 54%, 74% and 50% for the differential count.


CONCLUSIONS
The presence of bacterial infections in children can be tested using a simple slide test to reveal the increased state of leukocyte adhesiveness/aggregation in the peripheral blood. The LAAT is a reliable, rapid and inexpensive test, and it can be a useful laboratory tool for the paediatrician treating a child with acute febrile illness.
Urbach
J
J
Department of Pediatrics, Shaare Zedek Medical Center, Hebrew University, Medical School, Jerusalem: Israel.
Lebenthal
Y
Y
Levy
S
S
Berliner
S
S
Leibovitz
E
E
Aronson
M
M
Branski
D
D
D001424:Bacterial Infections / Q000097:blood* / Q000175:diagnosis*; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation; D002464:Cell Migration Inhibition; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007957:Leukocyte Adherence Inhibition Test; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology*; D008297:Male; D012680:Sensitivity and Specificity; D014766:Viremia / Q000097:blood* / Q000175:diagnosis*
D015815:Cell Adhesion Molecules