title: Metalloprotease-disintegrin (ADAM) genes are widely and differentially expressed in the adult CNS.
abstract: ADAM family of metalloprotease-disintegrins, including enzymes that process TNF-alpha and beta-amyloid precursor protein, has been indicated in neuronal development, but the role of these protease/adhesion/signaling proteins in adult nervous system remains poorly understood. Present study provides a systematic examination of ADAM gene expression in rodent CNS, showing the first quantitative characterization of ADAM mRNA distribution therein. At least 17 ADAM mRNAs were expressed. Individual ADAM mRNAs and their isoforms showed strikingly different expression patterns. Expression of mRNAs for ADAM10, the putative alpha-secretase, and ADAM17 (TACE), also indicated in APP processing, was further characterized using in situ hybridization. Expression of ADAM10 mRNA was widespread, while ADAM17 showed a more restricted pattern. Altogether, the wide and differential expression of ADAM mRNAs suggests versatile roles for ADAMs in the adult CNS.
authors.affiliation: Laboratory of Cancer Genetics, Institute of Medical Technology, Tampere, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Kärkkäinen
authors.forename: E
authors.initials: E
authors.lastname: Rybnikova
authors.forename: M
authors.initials: M
authors.lastname: Pelto-Huikko
authors.forename: A P
authors.initials: AP
authors.lastname: Huovila
mesh_terms: D051722:ADAM Proteins; D000072197:ADAM10 Protein; D000072198:ADAM17 Protein; D000367:Age Factors; D053829:Amyloid Precursor Protein Secretases; D000818:Animals; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry / Q000235:genetics*; D017931:DNA Primers; D019483:Disintegrins / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics; D008666:Metalloendopeptidases / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D017931:DNA Primers; D019483:Disintegrins; D008565:Membrane Proteins; D012333:RNA, Messenger; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; D008666:Metalloendopeptidases; D000072197:ADAM10 Protein; C491234:Adam10 protein, mouse; D000072198:ADAM17 Protein; C000606463:Adam17 protein, mouse