title: An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains.
abstract: To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.
authors.affiliation: Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
authors.lastname: van Dijken JP
authors.initials: J
authors.lastname: Bauer
authors.initials: L
authors.lastname: Brambilla
authors.initials: P
authors.lastname: Duboc
authors.initials: JM
authors.lastname: Francois
authors.initials: C
authors.lastname: Gancedo
authors.initials: ML
authors.lastname: Giuseppin
authors.initials: JJ
authors.lastname: Heijnen
authors.initials: M
authors.lastname: Hoare
authors.initials: HC
authors.lastname: Lange
authors.initials: EA
authors.lastname: Madden
authors.initials: P
authors.lastname: Niederberger
authors.initials: J
authors.lastname: Nielsen
authors.initials: JL
authors.lastname: Parrou
authors.initials: T
authors.lastname: Petit
authors.initials: D
authors.lastname: Porro
authors.initials: M
authors.lastname: Reuss
authors.lastname: van Riel N
authors.initials: M
authors.lastname: Rizzi
authors.initials: HY
authors.lastname: Steensma
authors.initials: CT
authors.lastname: Verrips
authors.initials: J
authors.lastname: Vindeløv
authors.initials: JT
authors.lastname: Pronk