title: The Monodelphis melanoma model: initial report on large ultraviolet A exposures of suckling young.
abstract: The objective of this study was to determine whether exposure of early suckling young of the opossum Monodelphis domestica to ultraviolet A (UVA) radiation (320-400 nm) can lead to the development of melanocytic lesions similar to those induced after exposure to ultraviolet B (UVB) radiation (280-320 nm) to total doses as low as 380 J/m2. A total of 576 sucklings received nine exposures of 0.6, 2.6 or 15.5 kJ/m2 per dose (total doses approximately 6, 23 and 140 kJ/m2, respectively) from a Blak Ray lamp source with a narrow range emission at 365 nm. A further 280 sucklings were exposed in the same way to doses of 2.6 kJ/m2 per dose (total approximately 23 kJ/m2) broad-band UVA with visible wavelengths from a Dermalight lamp. Frequency of litter loss following all of the UVA-exposure protocols was similar to that within the same stocks in the colony at large. Only one of the 856 UVA-exposed individuals possessed a melanocytic lesion at the 5 month assessment point. No radiation-induced lesions of any type were evident on the skin of the other animals exposed as sucklings. The affected male was from a group of 70 individuals exposed to the highest total dose (140 kJ/m2) from the Blak Ray light source. The melanocytic hyperplasia was provisionally identified as a potential melanoma but it slowly regressed as the animal aged. We conclude that in the opossum suckling exposure system, the potency of UVA for melanoma induction is extremely low compared with that of UVB. Possible explanations, amenable to further investigations, are given for the low UVA sensitivity of the suckling model compared to the adult exposure model of Ley (Ley, R. D. [1997] Cancer Res. 57, 3682-3684).
authors.affiliation: Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78245-0549, USA. edwardsr@darwin.sfbr.org
authors.forename: E S
authors.initials: ES
authors.lastname: Robinson
authors.forename: R H
authors.initials: RH
authors.lastname: Hill
authors.forename: M L
authors.initials: ML
authors.lastname: Kripke
authors.forename: R B
authors.initials: RB
authors.lastname: Setlow
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D004195:Disease Models, Animal; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology*; D009381:Neoplasms, Radiation-Induced / Q000473:pathology*; D009893:Opossums; D011829:Radiation Dosage; D012878:Skin Neoplasms / Q000473:pathology*; D014466:Ultraviolet Rays