Recovery of lymphocyte and dendritic cell subsets after autologous CD34+ cell transplantation.
Following high-dose chemotherapy (HDC) and peripheral blood progenitor cell transplantation (PBPCT), there are profound changes in leukocyte homeostasis and the immune system is compromised. Transplantation of purified CD34+ cells may further compromise immune recovery because the grafts are depleted of mature immune cells. However, a detailed monitoring of immune cell reconstitution has not been done. We monitored blood levels of antigen-presenting cells (APC) and of lymphocytes by multi-color flow cytometry at different times post CD34+ PBPCT. We found a rapid normalization of circulating levels of the antigen-presenting CD11c+ dendritic cells (defined as lineage- HLA-DR+ CD11c+ cells). There was a slight over-representation of lin- DR+ CD11c- cells at day 42 post transplantation suggesting that the composition of the APC population might be affected. Normal levels of total T, B and NK lymphocytes were rapidly achieved but the composition of the T cell population was abnormal. Patients had elevated levels of CD8+ T cells at early times and a persistent reduction in levels of naive CD8+ T cells (CD8+ CD4- CD45RA+ CD27+) and of naive CD4+ T cells (CD4+CD3+ CD8- CD45RA+). Thus, we found a rapid recovery of DC after CD34+ PBPCT but the specific numerical defects in naive T cells are likely to be a major cause of immune dysfunction in the patients. Bone Marrow Transplantation (2000) 25, 1249-1255.
Galy
A
A
Stem Cell Transplantation Program, Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA.
Rudraraju
S
S
Baynes
R
R
Klein
J
J
D000328:Adult; D017951:Antigen Presentation; D018952:Antigens, CD34; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology* / Q000628:therapy*; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D014182:Transplantation, Autologous
D018952:Antigens, CD34