Cellular distribution of isozymes of protein kinase C in septal olfactory epithelium of mice.
To determine the presence of protein kinase C (PKC) isozymes in the septal olfactory epithelium of mice (mSOE), western blotting and immunohistochemistry were performed using antibodies against PKC isozymes. With the exception of PKC-betaI, all of the PKC isozymes were detected in the whole lysate of septal tissue layer and apparent molecular weights for each isoform were found. PKC-alpha, PKC-gamma and PKC-epsilon were detected in the olfactory glandular cells of the lamina propria, and PKC-betaI and PKC-betaII were located in the microvillar cells. Neither novel PKC nor atypical PKC was detected in olfactory glandular cells or microvillar cells, except for PKC-epsilon. PKC-lambda was localized in the mucous layer of the mSOE. Meanwhile, PKC-delta and PKC-xi were distributed in the receptor cells in the mSOE. These data demonstrate the isoform-specific expression of PKC in mSOE and suggest a role for the novel and atypical types of PKC in olfactory transduction.
Kang
W S
WS
Department of Biology, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Lee
W K
WK
Seo
M S
MS
Kim
J K
JK
Han
J W
JW
Moon
Y W
YW
Cho
K K
KK
Kim
M J
MJ
Lee
K H
KH
Kim
Y S
YS
Jo
Y H
YH
Choi
C R
CR
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009300:Nasal Septum / Q000201:enzymology; D009475:Neurons, Afferent / Q000201:enzymology; D009831:Olfactory Mucosa / Q000201:enzymology*; D011493:Protein Kinase C / Q000096:biosynthesis / Q000378:metabolism*
D007527:Isoenzymes; D011493:Protein Kinase C