title: 2,3-DPG-Hb complex: a hypothesis for an asymmetric binding.
abstract: This study was undertaken to test the symmetry of 2,3-diphosphoglycerate (2,3-DPG) binding site in hemoglobin (Hb). From Arnone's study [A. Arnone, Nature (London) 237 (1972) 146] the 2,3-DPG binding site is located at the top of the cavity, that runs through the center of the deoxy-Hb molecule. However, it is possible that this symmetry reported by Arnone, for crystals of 2,3-DPG-Hb complex, might not be conserved in solution. In this paper, we report the 31P nuclear magnetic resonances of the 2,3-DPG interaction with Hb. The 2,3-DPG chemical shifts of the P2 and P3 resonance are both pH- and hemoglobin-dependent [protein from man, polar bear (Ursus maritimus), Arctic fox (Alopex lagopus) and bovine]. 2,3-DPG binds tightly to deoxyhemoglobin and weakly, nevertheless significantly, to oxyhemoglobin. In particular, our results suggest similar spatial position of the binding site of 2,3-DPG in both forms of Hb in solutions. However, the most unexpected result was the apparent loss of symmetry in the binding site, which might correlate with the ability of the hemoglobin to modulate its functional behavior. The different interactions of the phosphate groups indicate small differences in the quaternary structure of the different deoxy forms of hemoglobin. Given the above structural perturbation an asymmetric binding in the complex could justify, at least in part, different physiological properties of Hb. Regardless, functionally relevant effects of 2,3-DPG seem to be measured and best elucidated through solution studies.
authors.affiliation: Istituto di Chimica e Chimica Clinica, UCSC, Facoltà di Medicina, Roma, Italy. m.pomponi@uniserv.ccr.rm.cnr.it
authors.forename: M
authors.initials: M
authors.lastname: Pomponi
authors.forename: C
authors.initials: C
authors.lastname: Bertonati
authors.forename: E
authors.initials: E
authors.lastname: Fuglei
authors.forename: O
authors.initials: O
authors.lastname: Wiig
authors.forename: A E
authors.initials: AE
authors.lastname: Derocher
mesh_terms: D019794:2,3-Diphosphoglycerate / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D010108:Oxyhemoglobins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D012996:Solutions
chemical_list: D006454:Hemoglobins; D010108:Oxyhemoglobins; D012996:Solutions; D019794:2,3-Diphosphoglycerate; C100003:deoxyhemoglobin