Solution structure of a dynein motor domain associated light chain.
Dyneins are molecular motors that translocate towards the minus ends of microtubules. In Chlamydomonas flagellar outer arm dynein, light chain 1 (LC1) associates with the nucleotide binding region within the gamma heavy chain motor domain and consists of a central leucine-rich repeat section that folds as a cylindrical right handed spiral formed from six beta-beta-alpha motifs. This central cylinder is flanked by terminal helical subdomains. The C-terminal helical domain juts out from the cylinder and is adjacent to a hydrophobic surface within the repeat region that is proposed to interact with the dynein heavy chain. The position of the C-terminal domain on LC1 and the unexpected structural similarity between LC1 and U2A' from the human spliceosome suggest that this domain interacts with the dynein motor domain.
Wu
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Department of Biochemistry, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06032-3305, USA.
Maciejewski
M W
MW
Marintchev
A
A
Benashski
S E
SE
Mullen
G P
GP
King
S M
SM
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D016825:Chlamydomonas reinhardtii / Q000737:chemistry* / Q000166:cytology; D004398:Dyneins / Q000737:chemistry* / Q000378:metabolism*; D005407:Flagella / Q000737:chemistry; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017413:Ribonucleoprotein, U2 Small Nuclear / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D013329:Structure-Activity Relationship; D013499:Surface Properties
D004398:Dyneins; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D017413:Ribonucleoprotein, U2 Small Nuclear; D012996:Solutions; C484318:SNRPA1 protein, human