Characterization of CaGSP1, the Candida albicans RAN/GSP1 homologue.
Gsp1p is a small nuclear-located GTP binding protein from the yeast Saccharomyces cerevisiae. It is highly conserved among eucaryotic cells and is involved in numerous cellular processes, including nucleocytoplasmic trafficking of macromolecules. To learn more about the GSP1 structure/function, we have characterized its Candida albicans homologue. CaGsp1p is 214 amino acids long and displays 91% identity to the ScGsp1p. There is functional complementation in S. cerevisiae, and its mRNA is constitutively expressed in the diploid C. albicans grown under various physiological conditions. Disruption of both alleles was not possible, suggesting that it could be an essential gene, but heterozygous mutants exhibited genomic instability.
Clément
M
M
Department of Microbiology and Immunology, Université de Montréal, Centre-ville, Quebec, Canada.
Fournier
H
H
de Repentigny
L
L
Belhumeur
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D002176:Candida albicans / Q000235:genetics* / Q000254:growth & development; D002455:Cell Division / Q000235:genetics; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D005816:Genetic Complementation Test; D006160:Guanosine Triphosphate / Q000378:metabolism; D006579:Heterozygote; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic
C080324:GSP1 protein, S cerevisiae; D009687:Nuclear Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D006160:Guanosine Triphosphate; D020559:Monomeric GTP-Binding Proteins