title: Attempts to establish experimental Cyclospora cayetanensis infection in laboratory animals.
abstract: Attempts were made to develop an animal model for Cyclospora cayetanensis to identify a practical laboratory host for studying human cyclosporiasis. Oocysts collected from stool of infected humans in the United States, Haiti, Guatemala, Peru, and Nepal were held in potassium dichromate solution to allow development of sporozoites. The following animal types were inoculated: 9 strains of mice, including adult and neonatal immunocompetent and immune-deficient inbred and outbred strains, rats, sandrats, chickens, ducks, rabbits, jirds, hamsters, ferrets, pigs, dogs, owl monkeys, rhesus monkeys, and cynomolgus monkeys. Most animals were inoculated by gavage, although some of the primates were fed oocysts on food items. The animals were examined for signs of infection, particularly diarrhea, and stool samples were examined for 4-6 wk after inoculation. None of the animals developed patent infections or signs of infection. We conclude that none of the animals tested is susceptible to infection with C. cayetanensis.
authors.affiliation: Division of Parasitic Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia 30341, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Eberhard
authors.forename: Y R
authors.initials: YR
authors.lastname: Ortega
authors.forename: D E
authors.initials: DE
authors.lastname: Hanes
authors.forename: E K
authors.initials: EK
authors.lastname: Nace
authors.forename: R Q
authors.initials: RQ
authors.lastname: Do
authors.forename: M G
authors.initials: MG
authors.lastname: Robl
authors.forename: K Y
authors.initials: KY
authors.lastname: Won
authors.forename: C
authors.initials: C
authors.lastname: Gavidia
authors.forename: N L
authors.initials: NL
authors.lastname: Sass
authors.forename: K
authors.initials: K
authors.lastname: Mansfield
authors.forename: A
authors.initials: A
authors.lastname: Gozalo
authors.forename: J
authors.initials: J
authors.lastname: Griffiths
authors.forename: R
authors.initials: R
authors.lastname: Gilman
authors.forename: C R
authors.initials: CR
authors.lastname: Sterling
authors.forename: M J
authors.initials: MJ
authors.lastname: Arrowood
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004285:Dogs; D004372:Ducks; D016783:Eucoccidiida / Q000472:pathogenicity*; D005243:Feces / Q000469:parasitology; D005260:Female; D005289:Ferrets; D000882:Haplorhini; D006801:Humans; D008297:Male; D011817:Rabbits; D012377:Rodentia; D013552:Swine