Construction and characterization of monoclonal antibodies against western equine encephalitis virus.
A repertoire of mouse monoclonal antibodies (MAbs) against western equine encephalitis virus (WEE) was constructed and characterized. Anti-WEE antibodies were expressed from hybridomas and purified by protein G chromatography. Each of the antibodies was functionally assessed by indirect enzyme-linked immunosorbent assays (ELISAs), Western blotting, and immunoprecipitations. All antibodies bound to WEE antigen in ELISAs, whereas only a subgroup of antibodies was found to be active in Western blotting and immunoprecipitations. A subset of antibodies was found to cross-react with other alphaviruses, such as Sindbis virus (SIN), Venezuelan equine encephalitis (VEE), and eastern equine encephalitis (EEE). Because many of the antibodies were highly reactive to WEE antigen in one or more of the assays, these antibodies are excellent candidates for immunodetection and immunotherapy studies.
Long
M C
MC
Medical Countermeasures Section, Defence Research Establishment Suffield, Alberta, Canada.
Nagata
L P
LP
Ludwig
G V
GV
Alvi
A Z
AZ
Conley
J D
JD
Bhatti
A R
AR
Suresh
M R
MR
Fulton
R E
RE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D003429:Cross Reactions; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology; D004666:Encephalitis Virus, Venezuelan Equine / Q000276:immunology; D004667:Encephalitis Virus, Western Equine / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000737:chemistry; D007132:Immunoglobulin Isotypes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D012845:Sindbis Virus / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007132:Immunoglobulin Isotypes