Chemosensitivity of advanced larynx carcinoma cells in vitro and significance of multidrug resistance markers in these tumors.
Thirty cases of previously untreated advanced larynx carcinoma were checked for in vitro chemosensitivity and presence of the resistance markers viz. P-glycoprotein (P-gp) glutathione-S-transferase-pi (GST-pi) and protein kinase C (PKC) overexpression. The cytotoxicity testing was done using MTT assay and the resistance markers were checked by immunohistochemical methods using monoclonal antibodies. The drug combinations employed in MIT assay were 5FU* + MTX*, 5FU + cisPt*, 5FU + Mito*, cisPt + Mito and MTX + Mito (*5FU = 5Fluorouracil, MTX-methotrexate, cisPt-cisplatin and Mito = mitomycin C). No statistically significant correlation was observed between resistance to the above drug combinations and presence of the resistance markers under consideration. A statistically significant correlation was observed between node positivity and expression of resistance markers which indicates that presence of one or more of these markers in these tumors may be considered as a negative prognosis marker. CisPt-Mito was found to be the most effective drug combination in vitro, in the cases studied.
Juvekar
A S
AS
Cancer Research Institute, Parel, Mumbai, India.
Amonkar
A J
AJ
D'cruz
A K
AK
Saikaia
T
T
Pradhan
S
S
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis* / Q000235:genetics; D000970:Antineoplastic Agents / Q000633:toxicity*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000633:toxicity; D018432:Drug Resistance, Multiple; D005472:Fluorouracil / Q000633:toxicity; D015972:Gene Expression Regulation, Neoplastic; D005982:Glutathione Transferase / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology* / Q000601:surgery; D008207:Lymphatic Metastasis; D008727:Methotrexate / Q000633:toxicity; D016685:Mitomycin / Q000633:toxicity; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011493:Protein Kinase C / Q000032:analysis* / Q000235:genetics; D014407:Tumor Cells, Cultured
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D016685:Mitomycin; D005982:Glutathione Transferase; D011493:Protein Kinase C; D002945:Cisplatin; D005472:Fluorouracil; D008727:Methotrexate