Establishment of a ciliated epithelial cell line from human Fallopian tube.
Human tubal epithelial cells in primary culture were transfected with simian virus 40 (SV40) large T antigen plasmid, and an immortalized ciliated cell line, named as NT/T-S, was established without crisis. Transmission electron microscopy proved that NT/T-S cells had cilia, microvilli, junctional complexes, rough endoplasmic reticula, free ribosomes and microtubules. NT/T-S cells were evaluated preliminarily on the basis of co-culture study using surplus embryos at the 4- to 8-cell stage in our IVF and embryo transfer programme. All of the 133 embryos had >/=10% fragments (based on the surface area) and were unworthy of cryopreservation. Up to 57% (16/28) of the embryos with 10-30% fragments reached the blastocyst stage by co-culture. In contrast, blastocyst formation was observed in <10% of the control embryos, some of which were co-cultured with NFL/T cells (the immortalized human fetal liver epithelial cells) (1/16), and the others were incubated with the co-culture medium alone (1/18). Various cytokines/growth factors such as leukaemia inhibitory factor (LIF), interleukin (IL)-6, IL-8 and basic fibroblast growth factor were secreted by NT/T-S cells as well as by the tubal epithelial cells in primary culture. The establishment of a ciliated cell line will provide a valuable resource for the further studies of the Fallopian tube in the early events of pregnancy.
Ando
H
H
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Nagoya, Japan.ando@tsuru.med.nagoya-u.ac.jp
Kobayashi
M
M
Toda
S
S
Kikkawa
F
F
Masahashi
T
T
Mizutani
S
S
D001755:Blastocyst / Q000502:physiology; D002461:Cell Line, Transformed; D002923:Cilia / Q000648:ultrastructure; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003584:Cytological Techniques; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D005187:Fallopian Tubes / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology
D016207:Cytokines