title: Characterization of thin layers of glucose oxidase.
abstract: Glucose Oxidase (GOD) has been covalently bound to functionalized glass cover slips. The surface density of immobilized GOD molecules was measured by a method based on the amperometric determination of Flavin Adenine Dinucleotide (FAD). Atomic Force Microscopy (AFM) images, obtained in aqueous solution for the covalently bound enzyme, show a monomolecular layer of the enzyme on a functionalized glass surface. The catalytic constants were measured for the immobilized GOD and compared with those of the free enzyme.
authors.affiliation: Department of Biological Chemistry, University of Padova.
authors.forename: S
authors.initials: S
authors.lastname: Bortoluzzi
authors.forename: M L
authors.initials: ML
authors.lastname: Di Paolo
authors.forename: A
authors.initials: A
authors.lastname: Rigo
authors.forename: F
authors.initials: F
authors.lastname: Vianello
authors.forename: L
authors.initials: L
authors.lastname: Zennaro
mesh_terms: D005898:Glass; D005949:Glucose Oxidase / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005949:Glucose Oxidase