title: Effect of serial-day exposure to nitrogen dioxide on airway and blood leukocytes and lymphocyte subsets.
abstract: Nitrogen dioxide (NO2) is a free radical-producing oxidant gas. Inhalation of NO2 could cause airway inflammation, and decrease immune function. This experiment tested the hypothesis that exposure to NO2 would: 1) increase leukocytes in bronchoalveolar lavage (BAL); and 2) change the distribution of lymphocyte subsets and activation in BAL and peripheral blood (PB). Using a counter-balanced, repeated-measures design, 15 healthy volunteers were exposed to filtered air (FA) or 2.0 parts per million NO2 for 4 h x day(-1) (4 x 30 min of exercise), for three consecutive days. Bronchoscopy was performed 18 h following each exposure set, and PB was drawn pre-exposure and pre-bronchoscopy. Flow cytometry was used to enumerate lymphocyte subsets and activation makers in BAL and PB. In the bronchial fraction, there was an increase in the percentage of neutrophils following NO2 exposure compared to FA (median (interquartile range): 10.6 (4.8-17.2)% versus 5.3 (2.5-8.3)%; p=0.005). In the BAL, there was a decrease in the percentage of T-helper cells following NO2 exposure compared to FA (55.9 (40.8-62.7)% versus 61.6 (52.6-65.2)%; p=0.022). For PB, there were no between-condition differences in any leukocyte or lymphocyte subsets, or activation. In conclusion exposure to nitrogen dioxide results in bronchial inflammation and a minimal change in bronchoalveolar lavage T-helper cells, and no changes in peripheral blood cells.
authors.affiliation: Lung Biology Center, University of California, San Francisco 94143, USA.
authors.forename: C
authors.initials: C
authors.lastname: Solomon
authors.forename: D L
authors.initials: DL
authors.lastname: Christian
authors.forename: L L
authors.initials: LL
authors.lastname: Chen
authors.forename: B S
authors.initials: BS
authors.lastname: Welch
authors.forename: M T
authors.initials: MT
authors.lastname: Kleinman
authors.forename: E
authors.initials: E
authors.lastname: Dunham
authors.forename: D J
authors.initials: DJ
authors.lastname: Erle
authors.forename: J R
authors.initials: JR
authors.lastname: Balmes
mesh_terms: D000328:Adult; D001769:Blood; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D016131:Lymphocyte Subsets / Q000187:drug effects*; D008297:Male; D009585:Nitrogen Dioxide / Q000494:pharmacology*
chemical_list: D009585:Nitrogen Dioxide