[Transposon mutagenesis in preparing mutants of a strain--a producer of a new antibiotic batumin].
Various plasmids carrying transposon Tn5 were used to generate insertion mutants synthesizing batumin, a unique antibiotic with a selective antistaphylococcal effect. One of the plasmids used provided a sufficient yield of the clones in question. An analysis of over 7000 clones allowed us to select the mutant clones with increased and decreased levels of batumin synthesis and the mutants that lost the ability to synthesize this antibiotic.
Smirnov
V V
VV
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Churkina
L N
LN
Kravets
A N
AN
Perepnikhatka
V I
VI
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016296:Mutagenesis; D009930:Organic Chemicals; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics; D013210:Staphylococcus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D009930:Organic Chemicals; C088306:batumin
Transpozonovyĭ mutagenez pri poluchenii mutantov shtamma--produtsenta novogo antibiotika batumina.