title: Renal vascular morphology and haemodynamics in Dahl salt-sensitive rats on high salt-low potassium diet: neural and genetic influences.
abstract: OBJECTIVE
  A dietary combination of high salt and low potassium (HS-LK) exacerbates hypertension in Dahl salt-sensitive (DS) rats and renders Dahl salt-resistant (DR) rats hypertensive. In both strains, the hypertension is accompanied by remodelling of the renal resistance vasculature, and is attenuated by peripheral chemical sympathectomy. In the current study, we sought to determine whether the sympathetic nervous system is causally involved in mediating the renal vascular and haemodynamic alterations associated with HS-LK feeding in Dahl rats.
  
  
  METHODS
  Two groups each of DS and DR rats were maintained on HS-LK diet (8% NaCl, 0.2% KCl) for 8 weeks. One group of DS (n = 9) and DR (n = 8) were treated with 6-hydroxydopamine (6-OHDA) in 0.001 N HCl vehicle to chemically ablate peripheral sympathetic nerve terminals. The two remaining groups (n = 8 each) received equivalent injections of vehicle.
  
  
  METHODS
  At the end of the dietary regimen, arterial blood pressure (ABP), glomerular filtration rate (GFR) and renal blood flow (RBF) were measured, and the structure of intra-renal resistance vessels was examined by planar morphometric analysis of coronal sections prepared from perfusion-fixed kidneys.
  
  
  RESULTS
  Both 6-OHDA-treated and untreated DS rats presented a greater degree of intra-renal vessel remodelling characterized by reduced lumen diameter in the absence (eutrophic) or presence (hypertrophic) of cross-sectional area expansion, higher renal vascular resistance (RVR) and lower GFR and RBF than DR rats. Chemical sympathectomy increased lumen diameters and reduced vascular wall expansion, resulting in a decrease in RVR and a concomitant increase in RBF and GFR in both strains; however, the effect was more prominent in the DS rats.
  
  
  CONCLUSIONS
  We conclude that HS-LK-induced changes in intra-renal vessel structure and renal haemodynamic function in Dahl rats are, at least in part, dependent on the activity of the sympathetic nervous system.
authors.affiliation: Department of Physiology, University of Toronto, Ontario, Canada.
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.forename: J W
authors.initials: JW
authors.lastname: Scholey
authors.forename: H
authors.initials: H
authors.lastname: Sonnenberg
authors.forename: L G
authors.initials: LG
authors.lastname: Melo
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000294:innervation / Q000473:pathology / Q000503:physiopathology; D004032:Diet; D004039:Diet, Sodium-Restricted; D005919:Glomerular Filtration Rate / Q000187:drug effects; D006439:Hemodynamics; D008297:Male; D009420:Nervous System / Q000503:physiopathology*; D016627:Oxidopamine / Q000494:pharmacology; D011188:Potassium / Q000008:administration & dosage; D051381:Rats; D020303:Rats, Inbred Dahl / Q000235:genetics / Q000502:physiology*; D012079:Renal Circulation / Q000187:drug effects; D013563:Sympathectomy, Chemical; D013564:Sympathetic Nervous System / Q000503:physiopathology; D013565:Sympatholytics / Q000494:pharmacology; D014655:Vascular Resistance / Q000187:drug effects
chemical_list: D013565:Sympatholytics; D016627:Oxidopamine; D011188:Potassium