A metalloprotease disintegrin that controls cell migration in Caenorhabditis elegans.
In Caenorhabditis elegans, the gonad acquires two U-shaped arms by the directed migration of its distal tip cells (DTCs) along the body wall basement membranes. Correct migration of DTCs requires the mig-17 gene, which encodes a member of the metalloprotease-disintegrin protein family. The MIG-17 protein is secreted from muscle cells of the body wall and localizes in the basement membranes of gonad. This localization is dependent on the disintegrin-like domain of MIG-17 and its catalytic activity. These results suggest that the MIG-17 metalloprotease directs migration of DTCs by remodeling the basement membrane.
Nishiwaki
K
K
PRESTO, Japan Science and Technology Corporation and Fundamental Research Laboratories, NEC Corporation, Miyukigaoka, Tsukuba 305-8501, Japan.nishiwak@frl.cl.nec.co.jp
Hisamoto
N
N
Matsumoto
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001485:Basement Membrane / Q000201:enzymology; D017173:Caenorhabditis elegans / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins; D002465:Cell Movement; D003001:Cloning, Molecular; D019483:Disintegrins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D020869:Gene Expression Profiling; D017238:Genes, Helminth; D006031:Glycosylation; D006066:Gonads / Q000166:cytology / Q000201:enzymology / Q000254:growth & development; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009132:Muscles / Q000166:cytology / Q000201:enzymology; D009154:Mutation; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D019076:Transgenes
D029742:Caenorhabditis elegans Proteins; D019483:Disintegrins; C410348:MIG-17 protein, C elegans; D011993:Recombinant Fusion Proteins; C119674:GON-1 protein, C elegans; D008666:Metalloendopeptidases