Phytanyl-pyrophosphate-linked substrate for a bacterial alpha-mannosyltransferase.
The biochemical characterization of bacterial glycosyltransferases involved in the assembly of cell-wall-associated polysaccharides is often hindered by the lack of the appropriate undecaprenyl-pyrophosphate-linked acceptor substrate. In order to find a suitable synthetic substrate for the alpha1,3-mannosyltransferase AceA from Acetobacter xylinum, phytanyl-pyrophosphate-linked cellobiose was prepared. In the presence of GDP-[14C]mannose and recombinant AceA, the phytanyl-pyrophosphate-linked cellobiose afforded a 14C-labeled trisaccharide that was sensitive to alpha-mannosidase degradation in a fashion analogous to the natural undecaprenyl-pyrophosphate-linked cellobiose substrate. These results suggest that phytanyl-pyrophosphate-linked oligosaccharides may be useful substrates for other important bacterial glycosyltransferases.
Lellouch
A C
AC
CNRS Centre de Recherches sur les Macromolecules Vegetales, associe avec l'Universite Joseph Fourier, Grenoble, France. lellouch@cermav.cnrs.fr
Watt
G M
GM
Geremia
R A
RA
Flitsch
S L
SL
D000091:Acetobacter / Q000201:enzymology; D002475:Cellobiose / Q000737:chemistry / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D015394:Molecular Structure; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity
D011104:Polyisoprenyl Phosphate Oligosaccharides; D011994:Recombinant Proteins; D002475:Cellobiose; C100893:GDP-mannose - cellobiosyl-diphosphopolyprenol mannosyltransferase; D008364:Mannosyltransferases