Effects of lipid-derivatized glycosaminoglycans (GAGs), a novel probe for functional analyses of GAGs, on cell-to-substratum adhesion and neurite elongation in primary cultures of fetal rat hippocampal neurons.
The effects of glycosaminoglycans (GAG) on cell-to-substratum adhesion and neurite elongation were examined in primary cultures of fetal rat hippocampal neurons using tissue culture dishes coated with GAGs coupled to dipalmitoylphosphatidylethanolamine (PE), a novel probe for biological functions of GAGs. Both chondroitin sulfate conjugate to PE (CS-PE) and hyaluronic acid conjugate to PE (HA-PE) promoted neurite elongation from neurons in a dose-dependent manner when immobilized onto polylysine-coated dishes at various concentrations up to 1.0 microg/ml. The coating of CS-PE or HA-PE at a concentration higher than 1.0 microg/ml resulted in failure of neurite extension and adhesion of neurons to the substrata. In contrast, heparin conjugate to PE (HP-PE) did not exert any effects on neurite elongation or on cell attachment at these concentrations. These findings suggest that GAGs serve as a modulator for neurite elongation during neuronal network formation in the developing central nervous system.
Oohira
A
A
Department of Perinatology and Neuroglycoscience, Institute for Developmental Research, Kasugai, Aichi, Japan. oohira@inst-hsc.pref.aichi.jp
Kushima
Y
Y
Tokita
Y
Y
Sugiura
N
N
Sakurai
K
K
Suzuki
S
S
Kimata
K
K
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates; D005333:Fetus / Q000166:cytology; D006025:Glycosaminoglycans / Q000737:chemistry / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects*; D006820:Hyaluronic Acid; D015394:Molecular Structure; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000187:drug effects*; D010714:Phosphatidylethanolamines; D051381:Rats
D006025:Glycosaminoglycans; D010714:Phosphatidylethanolamines; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates