Preparation and protein conjugation of a divinyl sulphone derivatized bifunctional chelating agent.
A new bifunctional chelating agent with a novel linking arm, 2-[p-¿N-benzyl-N-(2-vinylsulfoethyl)¿- (aminobenzyl)¿-1,3-propane-diamine-N,N,N',N'-tetraacetic acid (VS-PDTA) was synthesized and was conjugated to protein for the purpose of attaching radiometals to monoclonal antibodies (MAbs). The effect of various parameters such as ligand concentration, protein concentration, pH, temperature and reaction period on the conjugation have been examined using chromatographic (SE and TLC) analysis after labeling with 111In. The parameters and chemical variables studied have significant effects on the efficiency and rate of protein conjugation.
Somayaji
V V
VV
AltaRex Inc., Edmonton, Alberta, Canada.
Naicker
S S
SS
Sykes
T R
TR
Guay
V
V
Noujaim
A A
AA
D002614:Chelating Agents / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000138:chemical synthesis*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000378:metabolism*; D013696:Temperature
D002614:Chelating Agents; D003432:Cross-Linking Reagents; D011506:Proteins