The TATA motif, the CAA motif and the poly(T) transcription termination motif are all important for transcription re-initiation on plant tRNA genes.
The effect of alteration of 5' and 3' flanking sequences on the transcription of plant tRNA genes was analysed using an RNA polymerase III-dependent in vitro transcription system derived from nuclei of cultured tobacco cells. A TATA-like sequence and the CAA motif frequently observed upstream of plant tRNA genes, and the poly(T) stretch usually present downstream, were shown to be necessary for efficient re-initiation of transcription. The CAA motif was shown to be a transcription initiation site. Introduction of the CAA and TATA-like motifs into a gene naturally lacking them greatly enhanced transcription by promoting efficient re-initiation.
Yukawa
Y
Y
Center for Gene Research, Nagoya University, Nagoya 464-8602, Japan.
Sugita
M
M
Choisne
N
N
Small
I
I
Sugiura
M
M
D001483:Base Sequence; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D010947:Plants, Toxic; D012362:RNA, Transfer, Ser / Q000235:genetics*; D017384:Sequence Deletion; D016385:TATA Box; D013728:Terminator Regions, Genetic / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D012362:RNA, Transfer, Ser