[Effect of suprainhibitory (supra-MIC) concentration of clindamycin on endotoxin release from Bacteroides fragilis].
The aim of this work was to determine the influence of clindamycin at concentration 100 x MIC on the growth of culture and the induction of endotoxin release from the cells of standard B. fragilis IPL E 323 strain. The antibiotic was added to 24-hour culture of the strain in BHI medium (time 0), its final concentration was 12.5 mg/l. The samples for further examinations were collected at time 0 and after 1, 2, 3 and 24 hours of continued incubation. At the same time the strain was cultured in a medium without the antibiotic. The optical density (OD 420 nm) of each sample was determined, they were centrifuged (2000 x g, 10 min), the supernatants were filtered (0.45 micron filters) and concentrated three times (5000 D ultrafilters). Two serological methods were applied to detect the presence of endotoxin in filtrates of culture medium: immunoelectroprecipitation (IEP) and immunoenzymatic assay (dot-ELISA) with rabbit anti-IPL E 323 immune serum. The results of experiments performed with filtrates of culture without the antibiotic indicate that standard B. fragilis IPL E 323 strain liberates endotoxin spontaneously to the culture medium. Single suprainhibitory dose of clindamycin at concentration 100 x MIC inhibits the growth of examined strain and does not cause augmented release of endotoxin from B. fragilis cells in vitro.
Rokosz
A
A
Zakład Mikrobiologii Lekarskiej AM w Warszawie.
Meisel-Mikołajczyk
F
F
D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001441:Bacteroides fragilis / Q000187:drug effects* / Q000378:metabolism*; D002981:Clindamycin / Q000008:administration & dosage*; D004731:Endotoxins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D008826:Microbial Sensitivity Tests; D011233:Precipitin Tests
D000900:Anti-Bacterial Agents; D004731:Endotoxins; D002981:Clindamycin
Wpływ nadprogowego (supra-MIC) stezenia klindamycyny na uwalnianie endotoksyny przez Bacteroides fragilis.