title: Differential secretion of adrenaline and noradrenaline in response to various secretagogues from bovine chromaffin cells.
abstract: 1. Differential secretion of adrenaline (Adr) and noradrenaline (NA) in response to various secretagogues was studied in bovine adrenal chromaffin cells. 2. Acetylcholine (ACh; 3-300 mumol/L), 1,1-dimethyl-4-phenyl-piperazinum (DMPP; 1-100 mumol/L), high K+ (20-60 mmol/L), calcimycin (1-100 mumol/L), histamine (0.3-30 mumol/L) and angiotensin (Ang)II (0.3-30 mumol/L) induced the secretion of a 1.3-2-fold greater percentage of NA stores than Adr stores in intact cells. 3. In beta-escin-permeabilized cells, Ca2+ (0.1-30 mumol/L) induced a greater secretion of Adr and NA in the presence of MgATP (2 mmol/L) than in the absence of MgATP. The percentage of NA secreted was 1.4- and 1.5-fold greater than that of Adr in the presence and absence of MgATP, respectively. 4. The ATP-independent phase of the Ca(2+)-dependent exocytosis is thought to be associated with the final step that ultimately leads to fusion, while the ATP-dependent phase is thought to be associated with the vesicle priming reaction. Therefore, the preferential secretion of NA in response to ACh, DMPP, high K+, calcimycin, histamine and AngII may be due, at least in part, to the greater effectiveness of Ca2+ in producing exocytosis in NA-containing cells.
authors.affiliation: Department of Dental Pharmacology, Nippon Dental University School of Dentistry at Niigata, Japan. kuwajima@ngt.ndu.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Kuwashima
authors.forename: C
authors.initials: C
authors.lastname: Matsumura
authors.forename: T
authors.initials: T
authors.lastname: Kimura
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000502:physiology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects; D019439:Chromaffin Cells / Q000187:drug effects* / Q000378:metabolism*; D004246:Dimethylphenylpiperazinium Iodide / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism*; D004928:Escin / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology
chemical_list: D000804:Angiotensin II; D000001:Calcimycin; D004246:Dimethylphenylpiperazinium Iodide; D004928:Escin; D006632:Histamine; D000255:Adenosine Triphosphate; D000109:Acetylcholine; D011188:Potassium; D001920:Bradykinin; D002118:Calcium; D009638:Norepinephrine; D004837:Epinephrine