[Integration of plasmids into E. coli K12 chromosomes, caused by a Bordetella transposon].
Transposition of Bordetella pertussis transposon in E. coli chromosome has been studied on a model of exclusion of donor multicopy pKK3 plasmid with coumermicin. TnBP3 induced the formation of co-integrates between the plasmid and chromosome. The structure of co-integrate was determined. Facts of exclusion of integrated structure and transposon transposition within integrated plasmid into new sites on a recipient chromosome were detected. Relationship between these processes and activity of bacterial cell recombination system has been determined.
Sivov
I G
IG
Beliavskiĭ
O A
OA
karataev
G I
GI
D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D017931:DNA Primers; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005819:Genetic Markers; D010957:Plasmids; D014161:Transduction, Genetic
D017931:DNA Primers; D004251:DNA Transposable Elements; D005819:Genetic Markers
Integratsiia plazmidy v khroosomu E. coli K12, obuslovlennaia transpozonom Bordetella.