[The development of a process for culturing Bordetella pertussis immobilized on a polyurethane carrier].
The processes of the cultivation of Bordetella pertussis, immobilized on polyurethane carrier in a fermenter, were carried out and studied. Acellular pertussis preparations were produced from the culture fluid obtained in the batch and multi-cycle cultivation processes with immobilized cells, as well as in the process with interrupted fermentation (for confirming the possibility of the preservation of cell viability). The content of protein and B. pertussis toxin in these preparations, as well as their leukocytes-stimulating and hemagglutinating activity, did not differ from similar characteristics of preparations obtained from culture fluid in homogeneous cultivation.
Miriasova
L V
LV
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Basnak'ian
I A
IA
Kopaeva
T V
TV
Sarkisov
I Iu
IIu
D001431:Bacteriological Techniques / Q000295:instrumentation; D019149:Bioreactors; D001886:Bordetella pertussis / Q000254:growth & development* / Q000378:metabolism; D018914:Cells, Immobilized; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D037342:Pertussis Toxin; D010567:Pertussis Vaccine; D011140:Polyurethanes; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000096:biosynthesis
D003470:Culture Media; D010567:Pertussis Vaccine; D011140:Polyurethanes; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin
Razrabotka protsessa kul'tivirovaniia kokliushnykh bakteriĭ, immobilizovannykh na poliuretanovom nositele.