Cloning and sequence analysis of the Candida boidinii ADE2 gene.
Candida boidinii ADE2 gene (phosphoribosyl-5-aminoimidazole carboxylase; AIRC, EC 4. 1. 1. 21) has been cloned by homology to the Saccharomyces cerevisiae ADE2 gene. The cloned C. boidinii ADE2 gene complemented the ade2 mutation of S. cerevisiae. Sequence analysis showed a single open reading frame of 1719 nucleotides coding for a polypeptide of 573 residues. Comparison of the deduced amino acid sequence with those of AIRC enzymes from other yeasts showed marked homology among yeast AIRCs.
Komeda
T
T
Central Laboratories for Key Technology, Kirin Brewery Co. Ltd., 1-13-5, Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa, 236-0004, Japan. t-komeda@kirin.co.jp
Suda
H
H
Sakai
Y
Y
Kato
N
N
Tamai
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D002262:Carboxy-Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA
D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase