title: Improved detection of microorganisms by polymerase chain reaction in delayed endophthalmitis after cataract surgery.
abstract: OBJECTIVE
  To evaluate whether the use of polymerase chain reaction (PCR) improves the identification of the causative pathogen in eyes developing delayed endophthalmitis after cataract surgery.
  
  
  METHODS
  Prospective, noncomparative case series.
  
  
  METHODS
  Consecutive series of 25 eyes with the clinical diagnosis of delayed endophthalmitis after cataract.
  
  
  METHODS
  Presence of bacterial or fungal DNA in aqueous humor and vitreous samples.
  
  
  RESULTS
  In the aqueous humor the causative pathogen was identified in 84% (n = 21) of the eyes by PCR compared with 0% by diagnostic culture and 0% by microscopy. In the vitreous samples the pathogen was identified in 92% (n = 23) of the eyes by PCR compared with 24% by diagnostic culture (n = 6) and 0% by microscopy.
  
  
  CONCLUSIONS
  PCR is useful for the identification of the causative pathogen in delayed endophthalmitis and had a higher rate of positive identification of the causative organism than microscopy or diagnostic culture.
authors.affiliation: Universitäts-Augenklinik, Regensburg, Germany.
authors.forename: C P
authors.initials: CP
authors.lastname: Lohmann
authors.forename: H J
authors.initials: HJ
authors.lastname: Linde
authors.forename: U
authors.initials: U
authors.lastname: Reischl
mesh_terms: D001082:Aqueous Humor / Q000382:microbiology; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D002387:Cataract Extraction / Q000009:adverse effects*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis*; D004271:DNA, Fungal / Q000032:analysis; D009877:Endophthalmitis / Q000382:microbiology*; D015818:Eye Infections, Bacterial / Q000209:etiology; D006801:Humans; D019654:Lens Implantation, Intraocular; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D014822:Vitreous Body / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004271:DNA, Fungal