Detection of human cytomegalovirus DNA by real-time quantitative PCR.
A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.
Nitsche
A
A
Klinik für Innere Medizin m.S. Hämatologie und Onkologie, Charité-Campus Virchow Klinikum, Humboldt Universität zu Berlin, Berlin, Germany.
Steuer
N
N
Schmidt
C A
CA
Landt
O
O
Ellerbrok
H
H
Pauli
G
G
Siegert
W
W
D001782:Blood Donors; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism; D019562:Viral Load
D004279:DNA, Viral; D019914:Taq Polymerase