Changes in REM sleep occurrence due to rhythmical auditory stimulation in the rat.
The effects of the rhythmical delivery of an auditory stimulus (1000 Hz, from 50 to 100 dB, 20 ms, every 20 s) on the pattern of rapid eye movement (REM) sleep occurrence was studied in the rat. The stimulation was simultaneously carried out on pairs of rats over 5 consecutive days (10-h recording sessions), during which a tone of increasing intensity (50, 63, 75, 88, 100 dB) was used. In each experimental session, auditory stimulation was triggered by the REM sleep occurrence of one rat (REMS-selective stimulation) whilst the other rat simultaneously received the same stimuli, but during any stage of the wake-sleep cycle (REMS-unselective stimulation). The results showed that the total amount of REM sleep in the 10-h recording session was increased over the 5 days of stimulation in the REMS-unselective group. This effect was due to an increase in the mean duration of REM sleep episodes. However, no significant changes were observed in animals under REMS-selective stimulation, nor in a third group of animals in which the spontaneous evolution of REM sleep occurrence (REMS-spontaneous) was studied. Since 86% of the stimuli under the REMS-unselective auditory stimulation fell outside REM sleep, the result would suggest that REM sleep occurrence is affected when the stimuli are delivered during a time period (i.e. during wakefulness or non-REM sleep) in which it is well known that physiological regulations are fully operant.
Amici
R
R
Dipartimento di Fisiologia Umana e Generale, Università di Bologna, Piazza di Porta S. Donato, 2, I-40127, Bologna, Italy. ramici@biocfarm.unibo.it
Domeniconi
R
R
Jones
C A
CA
Morales-Cobas
G
G
Perez
E
E
Tavernese
L
L
Torterolo
P
P
Zamboni
G
G
Parmeggiani
P L
PL
D000161:Acoustic Stimulation; D000818:Animals; D001306:Auditory Pathways / Q000502:physiology; D001921:Brain / Q000502:physiology; D004569:Electroencephalography; D008297:Male; D010507:Periodicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012895:Sleep, REM / Q000502:physiology*