Effects of an L-selectin antibody on the pulmonary and systemic manifestations of severe smoke inhalation injuries in sheep.
Sheep were treated with either lymphocyte adhesion molecule (LAM)1-3, an antibody against L-selectin, (40 mg 1 hour before smoke inhalation and 35 mg 24 hours after smoke inhalation; n = 6) or equivalent volumes of 0.9% saline solution (n = 6). After the smoke inhalation injuries, the PaO2/FIO2 ratio declined in both groups until 40 hours after the injuries, when a trend toward improvement was noted in the group that received LAM1-3. Lung lymph flow increased in both groups until 36 hours after the smoke inhalation injuries and then significantly decreased in the group that received LAM1-3. Forty-eight hours after the smoke inhalation injuries, there was a significant decrease in the ratio of wet-dry lung weight and in preservation of the reflection coefficient in the group that received LAM1-3 (P < .05). Histopathologic examination showed no differences between the groups in the pulmonary morphology associated with smoke inhalation. A reduction in splanchnic blood flow was noted in the control group (P < .05); this reduction was attenuated by treatment with LAM1-3. The delayed pulmonary effects and improved splanchnic blood flow suggested that LAM1-3 attenuated the development of a systemically induced secondary lung injury rather than of the primary lung injury associated with smoke inhalation.
Schenarts
P J
PJ
Department of Anesthesiology, University of Texas Medical Branch, and Shriners Burns Institute, Galveston 77555-0833, USA.
Schmalstieg
F C
FC
Hawkins
H
H
Bone
H G
HG
Traber
L D
LD
Traber
D L
DL
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D019041:L-Selectin / Q000494:pharmacology*; D008168:Lung / Q000473:pathology*; D008214:Lymphocytes / Q000276:immunology; D011652:Pulmonary Circulation; D051381:Rats; D012129:Respiratory Function Tests; D012756:Sheep; D015208:Smoke Inhalation Injury / Q000276:immunology* / Q000503:physiopathology
D019041:L-Selectin