title: In Euglena gracilis, a heat-shock protein related to hsc73 is constitutive and stress inducible.
abstract: Using monoclonal antibodies directed against different cytoplasmic isoforms of hsp70 proteins, namely, the constitutive hsc73 and the inducible hsp72 isoforms, we found that one isoform related to hsc73 was present in Euglena gracilis. This hsc73-like protein is expressed with a higher rate of synthesis in cells growing under heat shock than in control cells. Moreover, in cadmium-resistant cells, cultured at normal growth temperature, the rate of synthesis of this protein is constitutively increased. These results indicate that a heat-shock protein related to hsc73 is present in an ancestral eukaryote, Euglena gracilis, and that this protein may be constitutive and stress inducible as well.
authors.affiliation: Faculté de Pharmacie, Laboratoire Métabolisme Cellulaire et Xénobiotiques, Institut de Signalisation et Innovation Thérapeutique, Châtenay-Malabry, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Barque
authors.forename: P
authors.initials: P
authors.lastname: Schedler
authors.forename: E
authors.initials: E
authors.lastname: Floch
authors.forename: J
authors.initials: J
authors.lastname: Bonaly
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002104:Cadmium / Q000494:pharmacology; D004351:Drug Resistance; D015180:Electrophoresis, Gel, Two-Dimensional; D005056:Euglena gracilis / Q000187:drug effects / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D015800:Protozoan Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D015800:Protozoan Proteins; D002104:Cadmium