Coelichelin, a new peptide siderophore encoded by the Streptomyces coelicolor genome: structure prediction from the sequence of its non-ribosomal peptide synthetase.
A gene cluster for the non-ribosomal synthesis of a peptide of unknown structure has been identified in the partial genome sequence of Streptomyces coelicolor. Using molecular and computational analyses, the total structure of a tripeptide siderophore synthesized by the non-ribosomal peptide synthetase within the cluster has been deduced from the translated sequence of its encoding gene. This represents a novel method for the structural assignment of natural products from genome sequence data.
Challis
G L
GL
Department of Genetics, John Innes Centre, Norwich, UK. gchallis@ix.netcom.com
Ravel
J
J
D020816:Amino Acid Motifs; D019295:Computational Biology; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009842:Oligopeptides / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D010452:Peptide Biosynthesis; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017262:Siderophores / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D009842:Oligopeptides; D017262:Siderophores; C412862:coelichelin; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase