title: BCR/ABL rearrangement in Philadelphia chromosome negative CML patients.
abstract: Reverse transcription-polymerase chain reaction (RT-PCR) was used to study 34 patients with chronic myelogenous leukemia (CML) associated with negative Philadelphia (Ph) chromosome. This report showed evidence of a chimeric BCR/ABL transcript in 18 (52.9%) and 28 (82.4%) cases by first PCR and seminested PCR, respectively. In these BCR/ABL transcript positive cases, the incidence of BCR exon3/ABL exon2 (B3A2) and BCR exon 2/ABL exon2 rearrangement was 25 (89.3%) and 3 (10.7%) cases, respectively. The other 6 Ph negative patients showed no evidence of reciprocal translocation of BCR to chromosome 9. This data demonstrates that seminested PCR is sufficiently sensitive to detect BCR/ABL fusion transcript in Ph chromosome negative CML patients.
authors.affiliation: Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: B
authors.initials: B
authors.lastname: Rerkamnuaychoke
authors.forename: U
authors.initials: U
authors.lastname: Jomsawat
authors.forename: N
authors.initials: N
authors.lastname: Maneesri
authors.forename: A
authors.initials: A
authors.lastname: Apilugsanachit
authors.forename: N
authors.initials: N
authors.lastname: Pattanasak
mesh_terms: D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000032:analysis*; D005784:Gene Amplification; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics*; D008297:Male; D010677:Philadelphia Chromosome; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D016044:Fusion Proteins, bcr-abl