[Preparation of conjugated antigens based on zearalenone carboxymethyloxime and their use in immunoenzyme analysis].
Zearalenone-6'-carboxymethyloxime was synthesized, and its conjugates with albumins and gelatin were prepared. Polyclonal rabbit antibodies against the conjugate with bovine serum albumin were shown to be highly specific to zearalenone and to have a lower cross-reactivity toward its structural analogues (alpha-zearalenol--28%, beta-zearalenol--6%, zearalanone--12%, and alpha-zearalanol--5%). The sensitivity of enzyme immunoassay using gelatin-based immobilized conjugates for determination of zearalenone in solutions was 1 ng/ml, and this allowed us to determine this substance in feed at a threshold concentration of 200 micrograms/kg.
Burkin
A A
AA
All-Russia Institute of Veterinary Sanitation, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, Russia.
Konenenko
G P
GP
Soboleva
N A
NA
Zotova
E V
EV
D000418:Albumins / Q000737:chemistry; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D005780:Gelatin / Q000737:chemistry; D007124:Immunoenzyme Techniques; D010091:Oximes / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity; D015025:Zearalenone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology
D000418:Albumins; D000941:Antigens; D010091:Oximes; C411642:zearalenone 6'-carboxymethyloxime; D015025:Zearalenone; D005780:Gelatin
Poluchenie kon''iugirovannykh antigenov na osnove karboksimetiloksima zearalenona i ikh primenenie v immunofermentnom analize.