[Insertion of transposon P(lacW) with gradual expression of reporter genes].
In Drosophila, insertions of the P¿lacW¿ transposon led to uncommon expression patterns of the reporter genes white and lacZ: gene activity was gradually reduced in the neighboring cells along the dorsal-ventral or proximal-distal axes. Using in situ hybridization, the gradual gene expression was shown to be caused by P¿lacW¿ insertions in regions 40A (lines G1 and G2) and 69C (line G3) of the Drosophila genome. The expression patterns of genes lacZ and white were similar in eye cells of each line. In salivary gland polytene cells of G3 flies, the lacZ expression was also gradually reduced, which indicates the existence of a mechanism maintaining the gene activity gradient during endomitosis.
Alatortsev
V E
VE
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia. Alatortsev@img.ras.ru
Fadeeva
Iu V
IuV
Frolov
M V
MV
D000818:Animals; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics; D005123:Eye / Q000378:metabolism; D017930:Genes, Reporter; D017403:In Situ Hybridization; D007763:Lac Operon; D010858:Pigmentation / Q000235:genetics
D004251:DNA Transposable Elements
Insertsii transpozona P(lacW) s gradual'noĭ aktivnost'iu genov-reporterov.