Differentiation by polymerase chain reaction - restriction fragment length polymorphism of some Oestridae larvae causing myiasis.
The cytochrome oxidase I (COI) gene of the most wide-spread Italian species of Oestridae larvae causing myiasis (Gasterophilus spp., Hypoderma bovis, Hypoderma lineatum, Oestrus ovis and Przhevalskiana silenus) was amplified by polymerase chain reaction (PCR) using conserved primers. Restriction fragment length polymorphism (RFLP) of amplicons was also carried out and their restriction profiles compared. A clear genetic difference between the Oestridae larvae examined was demonstrated by using Taq(alpha) I, Hinf I, Rsa I and Hpa II enzymes. No intra-specific variation in RFLPs was detected between the two species of Hypoderma. The results highlight the taxonomic and phylogenetic relationships among larvae belonging to the different subfamilies, and thus offer additional diagnostic and epidemiological instruments.
Otranto
D
D
Dipartimento di Sanità e Benessere animale, Facoltà di Medicina Veterinaria, Valenzano, Italy.
Tarsitano
E
E
Giangaspero
A
A
Puccini
V
V
D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000302:isolation & purification; D019003:Deoxyribonuclease HpaII / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D004175:Diptera / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D009198:Myiasis / Q000469:parasitology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases; C040158:GTAC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases