title: Determination of histamine in tomatoes by liquid chromatography/mass spectrometry.
abstract: The histamine level in tomato fruits and pastes was determined by 2 orthogonal techniques as a means of comparing accuracy. Close statistical agreement was found between assays for free histamine by capillary electrophoresis (with UV absorbance detection), and for the dansyl derivative by reversed-phase liquid chromatography (LC). Both techniques have adequate sensitivity for the analysis of endogenous histamine in tomatoes, but LC/electrospray tandem mass spectrometry was more sensitive by at least an order of magnitude, down to a level of 0.05 mg/kg.
authors.affiliation: Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire, UK.
authors.forename: E
authors.initials: E
authors.lastname: Bolygo
authors.forename: P A
authors.initials: PA
authors.lastname: Cooper
authors.forename: K M
authors.initials: KM
authors.lastname: Jessop
authors.forename: F
authors.initials: F
authors.lastname: Moffatt
mesh_terms: D002853:Chromatography, Liquid; D006632:Histamine / Q000032:analysis*; D018551:Solanum lycopersicum / Q000737:chemistry*; D013058:Mass Spectrometry; D008956:Models, Chemical
chemical_list: D006632:Histamine