Usefulness of hybridization and PCR methods in monitoring of CMV infection in renal transplant recipients.
The purpose of this work was to compare hybridization and PCR methods as diagnostic tests in diagnosing and monitoring CMV infection. The investigation was performed in a group of 24 renal transplant recipients treated with ATG. The results we obtained suggest that quantitative variant of hybridization is more useful in diagnosing the infection than PCR, because it enables to monitor the infection. DNA CMV level of about 60 pg/ml or the increasing level in the subsequent samples should be a sign to start antiviral therapy.
Siennicka
J
J
Department of Virology, National Institute of Hygiene, Warsaw, Poland.
Durlik
M
M
Litwińska
B
B
Trzcińska
A
A
Samsel
R
R
Lewandowska
D
D
Paczek
L
L
Lao
M
M
Rowiński
W
W
Kańtoch
M
M
D000328:Adult; D000961:Antilymphocyte Serum / Q000627:therapeutic use; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011183:Postoperative Complications / Q000175:diagnosis; D015203:Reproducibility of Results
D000961:Antilymphocyte Serum; D004279:DNA, Viral; D007166:Immunosuppressive Agents