Solution properties of viilian, the exopolysaccharide from Lactococcus lactis subsp. cremoris SBT 0495.
The exopolysaccharide (EPS) "viilian" was isolated from a large-batch fermentation of Lactococcus lactis subsp. cremoris SBT 0495. After applying a newly developed purification procedure, pure viilian with a weight-averaged molar mass of 2.64 x 10(3) kg/mol was obtained in a yield of 0.6 g/L culture broth. The native EPS, as well as lower molar mass fractions obtained by sonication of the native polymer, were studied by capillary viscometry and size-exclusion chromatography (SEC) coupled to multiangle laser light scattering detection (MALLS). From the viscosity data at various ionic strengths, we extracted a Mark-Houwink-Kuhn-Sakurada exponent a = 0.79, and a Smidsrod B value of 0.03. By application of the Hearst, Bohdanecký, and Odijk models for stiff polymer coils, in connection to the experimental viscosity data, we established the characteristic ratio to be C(infinity) = 44 and the intrinsic persistence length q(0) = 11.5 nm. The rms radii of gyration predicted from each of the models were in good agreement with the experimental radii (e.g., <S(2)>(1/2)(w) = 162 nm for native viilian in 0.2M NaNO(3)), as determined by SEC-MALLS. In addition, the Odijk model predicts correct ionic strength-linear charge density dependence of the rms radius of gyration. From the combined viscosity and SEC-MALLS experiments we concluded that, in dilute aqueous solutions, viilian behaves as an intermediately stiff, random coil polyelectrolyte system.
Higashimura
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M
Snow Brand European Research Laboratories B.V., Zernikepark 6, 9747 AN Groningen, The Netherlands.
Mulder-Bosman
B W
BW
Reich
R
R
Iwasaki
T
T
Robijn
G W
GW
D002236:Carbohydrate Conformation; D002853:Chromatography, Liquid; D013294:Lactococcus lactis / Q000737:chemistry*; D008956:Models, Chemical; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012996:Solutions; D014783:Viscosity
D011135:Polysaccharides, Bacterial; D012996:Solutions; C411814:Viilian