In vitro and in vivo efficacy of lasalocid for treatment of experimental cryptosporidiosis.
In vitro viability of purified Cryptosporidium parvum oocysts, exposed for 30, 60, 90 and 120min to 0.27mg/ml lasalocid suspension was evaluated by inclusion or exclusion of two fluorogenic vital dyes and an excystation technique. Continuously, preventive and curative efficacies at different doses (9, 6.75, 5.625 and 4.5mg/kg body weight) and regimens of lasalocid against cryptosporidial infection were evaluated on an experimental neonatal mice model. In vitro assays demonstrated a decrease in the oocyst viability related to an increase in exposure time for exposure to the lasalocid suspension. The infection was eradicated when the suspension was administered with a dose of > or = 6.75mg/kg body weight. No apparent toxic effects were observed.
Castro Hermida
J A
JA
Laboratorio de Parasitología, Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, La Coruña, Spain.
Freire Santos
F
F
Oteiza López
A M
AM
Vergara Castiblanco
C A
CA
Ares-Mazás
M E
ME
D000818:Animals; D000831:Animals, Newborn; D003049:Coccidiostats / Q000008:administration & dosage / Q000592:standards / Q000627:therapeutic use*; D003457:Cryptosporidiosis / Q000188:drug therapy / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000187:drug effects / Q000254:growth & development*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D007420:Intestine, Large / Q000469:parasitology; D007421:Intestine, Small / Q000469:parasitology; D007832:Lasalocid / Q000008:administration & dosage / Q000592:standards / Q000627:therapeutic use*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000662:veterinary; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D010270:Parasite Egg Count / Q000662:veterinary; D011419:Propidium / Q000737:chemistry; D018709:Statistics, Nonparametric
D003049:Coccidiostats; D005456:Fluorescent Dyes; D007211:Indoles; D011419:Propidium; C007293:DAPI; D007832:Lasalocid