Effect of sodium butyric acid, sodium valerianic acid, and osmolarity on contractility of specimens of intestinal wall obtained from the cecum and spiral colon of healthy cows.
OBJECTIVE
To compare the effect of various concentrations of sodium butyric acid and sodium valerianic acid, as well as various osmolarities, on contractility of ex-vivo intestinal wall specimens obtained from the cecum and spiral colon of each of several healthy cows.


METHODS
Full-thickness preparations of intestinal wall, dissected parallel to the longitudinal smooth muscle layers, harvested from freshly slaughtered healthy cows.


METHODS
Specimens of intestinal wall were incubated for 5 minutes with various concentrations of sodium butyric acid and sodium valerianic acid as well as various osmolar concentrations of NaCl, using a crossover design. Isometric contractions were induced 7 times with carbachol (CH; 5 X 10(-6) mol/L). Contractility was defined as the maximum amplitude of contraction and the amplitude of contraction 2 minutes after addition of CH.


RESULTS
Repeated addition of CH did not result in a significant effect on contractility of specimens from the cecum and spiral colon. Contractility after addition of CH was not significantly affected by prior incubation with various concentrations of sodium butyric acid or sodium valerianic acid or after an increase of osmolarity. Maximum amplitude of contraction was significantly higher in specimens from the spiral colon, compared with specimens from the cecum.


CONCLUSIONS
Increases in concentrations of sodium butyric acid or sodium valerianic acid and increases in osmolarity did not inhibit contractility of intestinal wall specimens from the cecum and spiral colon of a group of healthy cows.
Allemann
M
M
Clinic for Food Animals and Horses, University of Bern, Switzerland.
Eicher
R
R
Mevissen
M
M
Haltiner
R
R
Meylan
M
M
Steiner
A
A
D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology*; D002217:Carbachol / Q000008:administration & dosage; D002417:Cattle; D002418:Cattle Diseases / Q000209:etiology / Q000503:physiopathology; D002432:Cecum / Q000187:drug effects* / Q000503:physiopathology; D018679:Cholinergic Agonists / Q000008:administration & dosage; D003106:Colon / Q000187:drug effects* / Q000503:physiopathology; D018592:Cross-Over Studies; D005260:Female; D066298:In Vitro Techniques; D007410:Intestinal Diseases / Q000209:etiology / Q000503:physiopathology / Q000662:veterinary; D015999:Multivariate Analysis; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009994:Osmolar Concentration; D010421:Pentanoic Acids / Q000494:pharmacology*; D018709:Statistics, Nonparametric
D018679:Cholinergic Agonists; D010421:Pentanoic Acids; D020148:Butyric Acid; D002217:Carbachol; C038780:n-pentanoic acid