Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA.
We analyzed the nucleotide sequences of the non-coding region of chloroplast DNA: the intergenic spacer between trnL (UAA) 3'exon and trnF (GAA). Two kinds of sequence, "type-1" and "type-2," were detected in 33 populations of Cannabis sativa. The length of the "type-1" fragment was 354 bp. In contrast, the "type-2" fragment from 3 populations was 353 bp long, with only one base deletion compared to "type-1." The fragment length from Humulus lupulus was 353 bp with a 1-bp deletion, and ten 1-bp substitutions compared to the sequences from C. sativa "type-1." Furthermore, we could clearly identify differences between C. sativa and H. lupulus using single-strand conformation polymorphism of PCR products (PCR-SSCP) analysis.
Kohjyouma
M
M
Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Shizuoka, Japan. kohjyou@nihs.go.jp
Lee
I J
IJ
Iida
O
O
Kurihara
K
K
Yamada
K
K
Makino
Y
Y
Sekita
S
S
Satake
M
M
D001483:Base Sequence; D002188:Cannabis / Q000235:genetics*; D017931:DNA Primers; D018742:DNA, Chloroplast / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D018742:DNA, Chloroplast