Correlation between metastatic potency and the down-regulation of E-cadherin in the mouse hepatoma cell lines G-1 and G-5.
Cell-cell adhesiveness, involving the adherens junction system including homophilic adhesion of cadherin and intracellular catenins, is a critical factor for tumor cell invasion and metastasis. We evaluated the levels of E-cadherin and beta-catenin in hepatoma cell sublines with high and low metastatic capacities. Stimulation of these cells with serum growth factors for more than 3 h after 24 h of starvation caused decreases in levels of E-cadherin and beta-catenin in the subline with high metastatic capacity, G-5. In contrast, no significant changes were observed in the subline with low metastatic capacity, G-1. Concomitantly with the decreases in E-cadherin and beta-catenin levels, G-5 cells were dissociated and detached from the culture dish, although G-1 cells again showed no morphological alterations. These in vitro results reflected the in vivo metastatic potencies of these hepatoma sublines, and further suggested the importance of the adherens junction system in determining metastatic potency of these parenchymal tumor cell lines as in epithelial/endothelial tumors.
Saeki
Y
Y
Department of Immunology and Cell biology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka 537-8511, Japan.
Hazeki
K
K
Matsumoto
M
M
Toyoshima
K
K
Akedo
H
H
Seya
T
T
D000818:Animals; D001564:Benzo(a)pyrene; D014408:Biomarkers, Tumor / Q000032:analysis; D015820:Cadherins / Q000032:analysis*; D002448:Cell Adhesion; D003598:Cytoskeletal Proteins / Q000032:analysis; D004622:Embryo, Mammalian; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D015534:Trans-Activators; D014407:Tumor Cells, Cultured; D051176:beta Catenin
D014408:Biomarkers, Tumor; C495267:CTNNB1 protein, mouse; D015820:Cadherins; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin; D001564:Benzo(a)pyrene