Relevance of IgG receptor IIIb (CD16) polymorphism to handling of Porphyromonas gingivalis: implications for the pathogenesis of adult periodontitis.
Polymorphonuclear neutrophils (PMNs) are essential in host defense against periodontopathic bacteria such as Porphyromonas gingivalis. The uptake of immunoglobulin G (IgG)-opsonized bacteria via IgG Fc receptors (Fc gamma R) on PMN constitutes a central defense mechanism in periodontium. Fc gamma RIIIb is the most abundantly expressed Fc gamma R on PMN and is functionally polymorphic. The Fc gamma RIIIb-NA1 and IIIb-NA2 allotypes interact differently with IgG1- and IgG3-opsonized particles. We recently showed recurrence rates of adult periodontitis (AP) to be higher in patients carrying at least 1 Fc gamma RIIIb-NA2 allele. In this study we evaluated the functional relevance of the Fc gamma RIIIb polymorphism to anti-P. gingivalis PMN effector functions. Our results showed Fc gamma RIIIb-NA2-carrying PMN from both patients with AP and healthy controls to be less efficient in phagocytosis and induction of oxidative burst upon interaction with IgG1- and IgG3-opsonized P. gingivalis. These functional differences between Fc gamma RIIIb-NA1 and IIIb-NA2 were observed in the presence of CD32-blocking antibody fragments, but not upon blocking CD16. Moreover, PMNs from AP patients exhibited increased Fc gamma RIIIb-allelic differences in IgG3-induced oxidative burst compared to control PMNs. These results support the concept that Fc gamma RIIIb heterogeneity may influence the clinical course of AP.
Kobayashi
T
T
Department of Periodontology, Faculty of Dentistry, Niigata University, Japan.
van der Pol
W L
WL
van de Winkel
J G
JG
Hara
K
K
Sugita
N
N
Westerdaal
N A
NA
Yoshie
H
H
Horigome
T
T
D000328:Adult; D019138:Antibodies, Blocking / Q000276:immunology; D016866:Bacteroidaceae Infections / Q000209:etiology* / Q000276:immunology; D005260:Female; D005838:Genotype; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology; D010518:Periodontitis / Q000209:etiology* / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D011110:Polymorphism, Genetic / Q000276:immunology*; D016966:Porphyromonas gingivalis / Q000276:immunology* / Q000472:pathogenicity; D017452:Receptors, IgG / Q000276:immunology*; D012008:Recurrence
D019138:Antibodies, Blocking; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D017452:Receptors, IgG