Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli.
Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.
Henne
A
A
Abteilung Allgemeine Mikrobiologie, Institut für Mikrobiologie und Genetik der Georg-August-Universität, 37077 Göttingen, Germany.
Schmitz
R A
RA
Bömeke
M
M
Gottschalk
G
G
Daniel
R
R
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D004950:Esterases / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012988:Soil Microbiology; D014280:Triglycerides / Q000378:metabolism; D014304:Triolein / Q000378:metabolism
D014280:Triglycerides; D014304:Triolein; D004950:Esterases; D008049:Lipase; C005830:tributyrin