Isolation and characterization of canthaxanthin biosynthesis genes from the photosynthetic bacterium Bradyrhizobium sp. strain ORS278.
A carotenoid biosynthesis gene cluster involved in canthaxanthin production was isolated from the photosynthetic Bradyrhizobium sp. strain ORS278. This cluster includes five genes identified as crtE, crtY, crtI, crtB, and crtW that are organized in at least two operons. The functional assignment of each open reading frame was confirmed by complementation studies.
Hannibal
L
L
Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA, 34398 Montpellier, France.
Lorquin
J
J
D'Ortoli
N A
NA
Garcia
N
N
Chaintreuil
C
C
Masson-Boivin
C
C
Dreyfus
B
B
Giraud
E
E
D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D020369:Bradyrhizobium / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D016644:Canthaxanthin / Q000096:biosynthesis*; D002338:Carotenoids / Q000096:biosynthesis; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000378:metabolism; D005798:Genes, Bacterial; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D019753:Intramolecular Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010088:Oxidoreductases / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D002338:Carotenoids; D016644:Canthaxanthin; D010088:Oxidoreductases; D010105:Oxygenases; C093259:beta-carotene ketolase; C022398:phytoene dehydrogenase; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D019753:Intramolecular Lyases; C070925:lycopene cyclase-isomerase