title: Characterization of protease-activated receptors in rat peritoneal mast cells.
abstract: Activation of protease-activated receptor (PAR)-1 or PAR-2 elicits inflammation most probably via mast cell degranulation in vivo. The present study aimed at characterizing PARs in rat peritoneal mast cells (PMC). Messenger RNA for PAR-1, but not for PAR-2, was detected in PMC. Thrombin, the PAR-1 agonist SFLLR-NH2 or the PAR-2 agonist SLIGRL-NH2 failed to induce histamine release from PMC. Surprisingly, the PAR-2-inactive control peptide LSIGRL-NH2 triggered histamine release from PMC. Thus, PAR-1, but not PAR-2, are expressed in PMC, whereas neither PAR-1 nor PAR-2 are considered to be involved in degranulation of PMC. LSIGRL-NH2 does not appear to be appropriate as a control peptide for PAR-2 in inflammation studies.
authors.affiliation: Research & Development Center, Fuso Pharmaceutical Industries Ltd., Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nishikawa
authors.forename: A
authors.initials: A
authors.lastname: Kawabata
authors.forename: R
authors.initials: R
authors.lastname: Kuroda
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: K
authors.initials: K
authors.lastname: Kawai
mesh_terms: D000818:Animals; D029742:Caenorhabditis elegans Proteins; D015550:Cell Degranulation / Q000187:drug effects; D015801:Helminth Proteins / Q000235:genetics / Q000502:physiology*; D006636:Histamine Release / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D010529:Peritoneal Cavity / Q000166:cytology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013917:Thrombin / Q000494:pharmacology
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D012333:RNA, Messenger; C087977:par-2 protein, C elegans; D017346:Protein Serine-Threonine Kinases; D013917:Thrombin