title: A closer look at the insertion within helix 54 of the 23S-rRNA of the genus Corynebacterium (and related taxa).
abstract: Direct sequencing of the insertion in helix 54 of the 23S-rRNA of different strains of 8 species of the genus Corynebacterium was performed in order to determine the extent of variations of these strains. The results demonstrated that there is considerable variation within different strains of a single species. While this clearly jeopardizes the usefulness of the insertion with regard to species-specific diagnostic probes, the variations were found to concentrate within two clearly defined regions comprising the ascending and descending parts of the distal helix of a putative stemlike secondary structure. The remainder of the insertion sequences seem to be fairly constant within a single species while displaying considerable differences toward sequences of other species of the same genus.
authors.affiliation: Laborärztliche Gemeinschaftspraxis Heidelberg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hügle
authors.forename: M
authors.initials: M
authors.lastname: Weizenegger
authors.forename: K
authors.initials: K
authors.lastname: Hengstler
authors.forename: A
authors.initials: A
authors.lastname: Fahr
authors.forename: J
authors.initials: J
authors.lastname: Bartel
mesh_terms: D001483:Base Sequence; D003352:Corynebacterium / Q000145:classification / Q000235:genetics*; D004251:DNA Transposable Elements; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000737:chemistry / Q000235:genetics; D009690:Nucleic Acid Conformation; D015347:RNA Probes / Q000737:chemistry; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements; D015347:RNA Probes; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S