Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1.
An assay was developed to study plant receptor kinase activation and signaling mechanisms. The extracellular leucine-rich repeat (LRR) and transmembrane domains of the Arabidopsis receptor kinase BRI1, which is implicated in brassinosteroid signaling, were fused to the serine/threonine kinase domain of XA21, the rice disease resistance receptor. The chimeric receptor initiates plant defense responses in rice cells upon treatment with brassinosteroids. These results, which indicate that the extracellular domain of BRI1 perceives brassinosteroids, suggest a general signaling mechanism for the LRR receptor kinases of plants. This system should allow the discovery of ligands for the LRR kinases, the largest group of plant receptor kinases.
He
Z
Z
Plant Biology Laboratory, The Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Wang
Z Y
ZY
Li
J
J
Zhu
Q
Q
Lamb
C
C
Ronald
P
P
Chory
J
J
D017360:Arabidopsis; D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D016923:Cell Death; D002460:Cell Line; D002688:Chitinases / Q000235:genetics; D002777:Cholestanols / Q000378:metabolism* / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D008024:Ligands; D012275:Oryza / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016897:Respiratory Burst; D015398:Signal Transduction; D013260:Steroids, Heterocyclic / Q000378:metabolism* / Q000494:pharmacology; D014974:Xanthomonas / Q000502:physiology
D029681:Arabidopsis Proteins; D060406:Brassinosteroids; D002777:Cholestanols; D008024:Ligands; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D013260:Steroids, Heterocyclic; C097148:Xa21 protein, Oryza sativa; D011494:Protein Kinases; C108270:BRI1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; D002688:Chitinases; D010650:Phenylalanine Ammonia-Lyase; C023623:brassinolide