An AraC-like transcriptional activator is required for induction of genes needed for alpha-galactoside utilization in Sinorhizobium meliloti.
The nodulating bacterium Sinorhizobium meliloti can utilize alpha-galactosides like melibiose and raffinose as sole sources of carbon and energy. We show that this utilization requires an AraC-like transcriptional activator, AgpT. When agpT was inactivated, Rhizobium meliloti could not utilize alpha-galactosides or induce genes required for transport and catabolism of these sugars. The agpT gene was not essential for the establishment of an effective nitrogen-fixing symbiosis.
Bringhurst
R M
RM
University of Connecticut, Department of Molecular and Cell Biology, 75 N. Eagleville Rd., U-44, Storrs, CT 06269, USA.
Gage
D J
DJ
D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D003470:Culture Media; D005697:Galactosides / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012097:Repressor Proteins / Q000737:chemistry / Q000378:metabolism; D017422:Sequence Analysis, DNA; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000378:metabolism*; D013559:Symbiosis; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors
C412824:AgpT protein, Sinorhizobium meliloti; D051777:AraC Transcription Factor; D001426:Bacterial Proteins; D003470:Culture Media; D005697:Galactosides; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000469:Alkaline Phosphatase