title: A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding.
abstract: The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competitive binding using NLSs immobilized on the solid phase (TentaGel resin).
authors.affiliation: Department of Chemistry, Texas A&M University, College Station 77842-3012, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Connolly
authors.forename: S B
authors.initials: SB
authors.lastname: Park
authors.forename: B M
authors.initials: BM
authors.lastname: Reedy
authors.forename: R F
authors.initials: RF
authors.lastname: Standaert
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry; D001667:Binding, Competitive / Q000187:drug effects; D001710:Biotin / Q000737:chemistry; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003124:Colorimetry; D007202:Indicators and Reagents; D007211:Indoles / Q000737:chemistry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects / Q000378:metabolism*; D019809:Streptavidin; D028901:alpha Karyopherins
chemical_list: D000952:Antigens, Polyomavirus Transforming; D007202:Indicators and Reagents; D007211:Indoles; D009687:Nuclear Proteins; D010455:Peptides; D018160:Receptors, Cytoplasmic and Nuclear; D028901:alpha Karyopherins; C035455:5-bromo-4-chloro-3-indoxyl phosphate; D001710:Biotin; D019809:Streptavidin; D000469:Alkaline Phosphatase