Amplification of human genomic sequences by human papillomaviruses universal consensus primers.
Detection of human papillomaviruses DNA (HPV) in tumour samples is often determined by a PCR based approach with standard universal consensus oligonucleotides. It is shown that these primers under the same amplification conditions can amplify a human genomic sequence of 1361 nucleotides in oral carcinomas and normal DNA samples. This sequence is detected more easily as the copy number of HPV DNA decreases. Therefore, in tumour samples that have a low copy number of HPV or that are contaminated by normal tissue there is a potential risk of misidentification of the presence of HPV if this observation is not taken into account.
Fernández-Contreras
M E
ME
Unidad de Investigación, Servicio de Medicina Interna, Hospital Universitario de La Princesa, Madrid, Spain.
Sarriá
C
C
Nieto
S
S
Lazo
P A
PA
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis*; D005189:False Positive Reactions; D006367:HeLa Cells; D019337:Hematologic Neoplasms / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000235:genetics* / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014412:Tumor Virus Infections
D017931:DNA Primers; D004279:DNA, Viral