Distinctive properties of the catalase B of Aspergillus nidulans.
Aspergillus nidulans catalase B (CatB) was purified to homogeneity and characterized as a hydroperoxidase which resembles typical catalases in some physicochemical characteristics: (1) it has an apparent molecular weight of 360000 and is composed of four glycosylated subunits, (2) it has hydrophobic properties as revealed by extractability in ethanol/chloroform and binding to phenyl-Superose, and (3) it has an acidic isoelectric point at pH 3. 5. Also CatB exhibits some distinctive properties, e.g. it is not inhibited by the presence of 2% sodium dodecyl sulfate, 9 M urea or reducing agents. Furthermore, even though CatB does not exhibit any residual peroxidase activity, it is able to retain up to 38% of its initial catalase activity after incubation with the typical catalase inhibitor 3-amino-1,2,4-triazole.
Calera
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JA
Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Sánchez-Weatherby
J
J
López-Medrano
R
R
Leal
F
F
D000640:Amitrole / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001233:Aspergillus nidulans / Q000201:enzymology*; D002374:Catalase / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000494:pharmacology; D010544:Peroxidases / Q000378:metabolism; D019163:Reducing Agents / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D013997:Time Factors; D014415:Tunicamycin / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D019163:Reducing Agents; D013501:Surface-Active Agents; D014415:Tunicamycin; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D010544:Peroxidases; D002374:Catalase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D000640:Amitrole