Two N-terminally truncated forms of C-type natriuretic peptide from habu snake venom.
Two N-terminally truncated forms of the C-type natriuretic peptide (CNP) were isolated from the venom of habu snake, Trimeresurus flavoviridis, and their structures were determined by EMI-MS spectrometry and amino acid sequencing. Tf-CNP(6-22), the shorter peptide retaining the 17-membered ring structure formed by an intra-molecular disulfide bridge, has a vasorelaxant activity in rat aortic strips and a diuretic potency in anesthetized rats. Tf-CNP(3-22), the other 20 amino acid residues peptide, also comprised the 17- membered ring with a short N-terminal extension of 3 amino acid residues. Tf-CNP(6-22), the ring, is the shortest naturally occurring CNP peptide identified so far, and as potent as Tf-CNP(1-22), the supposedly intact CNP of 22 amino acid residues.
Michel
G H
GH
School of Pharmaceutical Sciences, Showa University, Hatanodai, Shinagawa-ku, 142-8555, Tokyo, Japan.
Murayama
N
N
Sada
T
T
Nozaki
M
M
Saguchi
K
K
Ohi
H
H
Fujita
Y
Y
Koike
H
H
Higuchi
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000737:chemistry* / Q000494:pharmacology; D004231:Diuresis / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D017841:Trimeresurus; D014664:Vasodilation / Q000187:drug effects
D003435:Crotalid Venoms; D020098:Natriuretic Peptide, C-Type