[Topography of cell ahesion molecules CD9, CD24, L1 and N-CAM on the surface of neuroblastoma cells studied using chemical cross linking].
Cell-adhesion molecules are thought to play crucial roles in development and plasticity in the nervous system. Four neural cell adhesion molecules CD9, CD24, L1 and N-CAM are associated in the surface membrane of cultured neuroblastoma cells as studied by chemical cross-linking with bifunctional reagent 3,3'-dithiobis (sulphosuccinimidyl-propionate) followed by a subsequent immunodetection using antibodies directed against the above molecules. We obtained direct evidence of CD9 and L1, but not CD9 and N-CAM clasterisation, also interactions of CD24 with L1, CD24 with N-CAM and some others. These observations illustrate topography of neural cell adhesion molecules located in the vicinity to each other and imply the basis for their functional cooperativity.
Liakhovich
A V
AV
Center for Molecular Neurobiology Eppendorf, Hamburg, Germany.
Aksenov
N L
NL
D000906:Antibodies / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000906:Antibodies; D015815:Cell Adhesion Molecules; D003432:Cross-Linking Reagents
Izuchenie topografii molekul kletochnoĭ adgezii CD9, CD24, L1 i N-CAM na poverkhnosti neĭroblastomnykh kletok s pomoshch'iu metoda khimicheskikh sshivok.