title: Short-term storage of human haematopoietic cells. Influence of air and deoxyribonuclease I.
abstract: The aim of this study was to optimize strategy for ex vivo short-term storage of human cord blood and bone marrow haematopoietic cells. We report that the presence of air in the vials (1/2 of their volume), in which hematopoietic cells are stored, improves the survival of clonogenic progenitors. We observed that presence of air prevented a rapid decrease in the pH of storage medium. Similarly, a beneficial effect on cell survival and recovery also had an addition of Deoxyribonuclease I (DNase I). We observed that DNase I efficiently prevented cell clumping, and moreover, did not affect the clonogenecity of the haematopoietic progenitors. Therefore containers, in which haematopoietic cells are stored, should contain enough air (source of oxygen) and the storage medium itself should be supplemented with DNase I.
authors.affiliation: Department of Cell Pathology, Pomeranian School of Medicine, Szczecin, Poland. machalin@r1.pam.szczecin.pl
authors.forename: B
authors.initials: B
authors.lastname: Machaliński
authors.forename: P
authors.initials: P
authors.lastname: Szołomicka
authors.forename: J
authors.initials: J
authors.lastname: Kijowski
authors.forename: M
authors.initials: M
authors.lastname: Baśkiewicz
authors.forename: A
authors.initials: A
authors.lastname: Karbicka
authors.forename: E
authors.initials: E
authors.lastname: Byra
authors.forename: M
authors.initials: M
authors.lastname: Majka
authors.forename: S
authors.initials: S
authors.lastname: Giedrys-Kalemba
authors.forename: M Z
authors.initials: MZ
authors.lastname: Ratajczak
mesh_terms: D000388:Air; D001854:Bone Marrow Cells / Q000166:cytology*; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D003470:Culture Media; D003850:Deoxyribonuclease I; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D011247:Pregnancy; D011309:Preservation, Biological / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D003470:Culture Media; D003850:Deoxyribonuclease I