title: A novel HLA-B*40 allele and novel exon 1 sequences of two B*40 alleles identified in potential marrow donors.
abstract: Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) typing suggested the presence of variants of HLA-B*40 in three individuals. Two were part of 3,500 potential marrow donors being screened for the National Marrow Donor Program, while the third was a clinical specimen. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. In one individual, a native of the Pacific Islands, sequencing revealed a novel HLA-B*40 allele (B*4023). In two other individuals, a previously unknown exon 1 sequence was determined for HLA-B*4016 (ethnicity unknown) and B*4020 (Hispanic). These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19305, USA.
authors.forename: I S
authors.initials: IS
authors.lastname: Gourley
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Bassinger
authors.forename: G D
authors.initials: GD
authors.lastname: Montoya
authors.forename: J
authors.initials: J
authors.lastname: Yee
authors.forename: B B
authors.initials: BB
authors.lastname: Griffith
authors.forename: J
authors.initials: J
authors.lastname: Kearns
authors.forename: M
authors.initials: M
authors.lastname: McKeen
authors.forename: S
authors.initials: S
authors.lastname: Birkos
authors.forename: T M
authors.initials: TM
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Kamoun
mesh_terms: D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005091:Exons / Q000235:genetics*; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012042:Registries; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens