title: [Effect of lipids on I and III procollagen mRNA expression of hepatic stellate cells].
abstract: OBJECTIVE
  To study the effect of lipids on I and III procollagen mRNA expression of hepatic stellate cells (HSC).
  
  
  METHODS
  HSC were isolated and cultured from the liver of Wistar rats by in situ perfusion with pronase and collagenase and the density gradient centrifugation with Nycodenz. HSC were incubated with triglyceride (25mg/L) and very low-density lipid (VLDL, 25 mg/L) for 10d, respectively. I and III procollagen mRNA expression of HSC was detected by Northern blot hybridization.
  
  
  RESULTS
  After HSC was stimulated with triglyceride and VLDL, the expression of I and III procollagen mRNA increased obviously.
  
  
  CONCLUSIONS
  Lipids may promote the expression of I and III procollagen mRNA, and may be associated with fatty liver and hepatic fibrogenesis.
authors.affiliation: Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
authors.forename: L
authors.initials: L
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Zeng
authors.forename: J
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D005234:Fatty Liver / Q000209:etiology*; D008055:Lipids / Q000494:pharmacology*; D008079:Lipoproteins, VLDL / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011347:Procollagen / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000494:pharmacology
chemical_list: D008055:Lipids; D008079:Lipoproteins, VLDL; D011347:Procollagen; D012333:RNA, Messenger; D014280:Triglycerides