A cellular protein with an RNA-binding activity co-purifies with viral dsRNA from mycovirus-infected Helminthosporium victoriae.
A cellular protein that co-purifies with mycoviral dsRNA was isolated from the plant pathogenic fungus Helminthosporium victoriae (telomorph: Cochliobolus victoriae) infected with two viruses, the totivirus Helminthosporium victoriae 190S virus and the chrysovirus-like Helminthosporium victoriae 145S virus (Hv145SV). The cellular protein, which was, designated Hv-p68, accumulated to higher levels in virus-infected isolates compared to virus-free ones. The majority of the Hv145S dsRNAs were found in association with Hv-p68 and not packaged in virions. Hv-p68 could also be detected as a minor component of the virus capsid. Evidence is presented that Hv-p68 occurs in vivo as an octamer and that it possesses RNA-binding activities. Based on partial amino acid sequence analysis, Hv-p68 was shown to share significant sequence identity with alcohol oxidases from methylotrophic yeasts. Hv-p68 is proposed to play a role in viral RNA packaging/replication and in regulating viral pathogenesis.
Soldevila
A I
AI
Department of Plant Pathology, University of Kentucky, Lexington, Kentucky 40546-0091, USA.
Havens
W M
WM
Ghabrial
S A
SA
D000429:Alcohol Oxidoreductases / Q000737:chemistry; D000595:Amino Acid Sequence; D002213:Capsid / Q000378:metabolism / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D006375:Helminthosporium / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D015347:RNA Probes / Q000235:genetics / Q000378:metabolism; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism* / Q000648:ultrastructure; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019065:Virus Assembly
D015347:RNA Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase