title: Myelin/oligodendrocyte glycoprotein-induced autoimmune encephalomyelitis in common marmosets: the encephalitogenic T cell epitope pMOG24-36 is presented by a monomorphic MHC class II molecule.
abstract: Immunization of common marmosets (Callithrix jacchus) with a single dose of human myelin in CFA, without administration of Bordetella pertussis, induces a form of autoimmune encephalomyelitis (EAE) resembling in its clinical and pathological expression multiple sclerosis in humans. The EAE incidence in our outbred marmoset colony is 100%. This study was undertaken to assess the genetic and immunological basis of the high EAE susceptibility. To this end, we determined the separate contributions of immune reactions to myelin/oligodendrocyte glycoprotein (MOG) and myelin basic protein to the EAE induction. Essentially all pathological features of myelin-induced EAE were also found in animals immunized with MOG in CFA, whereas in animals immunized with myelin basic protein in CFA clinical and pathological signs of EAE were lacking. The epitope recognition by anti-MOG Abs and T cells were assessed. Evidence is provided that the initiation of EAE is based on T and B cell activation by the encephalitogenic phMOG14-36 peptide in the context of monomorphic Caja-DRB*W1201 molecules.
authors.affiliation: Department of Immunobiology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors.forename: H P
authors.initials: HP
authors.lastname: Brok
authors.forename: A
authors.initials: A
authors.lastname: Uccelli
authors.forename: N
authors.initials: N
authors.lastname: Kerlero De Rosbo
authors.forename: R E
authors.initials: RE
authors.lastname: Bontrop
authors.forename: L
authors.initials: L
authors.lastname: Roccatagliata
authors.forename: N G
authors.initials: NG
authors.lastname: de Groot
authors.forename: E
authors.initials: E
authors.lastname: Capello
authors.forename: J D
authors.initials: JD
authors.lastname: Laman
authors.forename: K
authors.initials: K
authors.lastname: Nicolay
authors.forename: G L
authors.initials: GL
authors.lastname: Mancardi
authors.forename: A
authors.initials: A
authors.lastname: Ben-Nun
authors.forename: B A
authors.initials: BA
authors.lastname: Hart
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D001323:Autoantibodies / Q000096:biosynthesis; D002144:Callithrix; D002460:Cell Line; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007114:Immunization; D007271:Injections, Intradermal; D008213:Lymphocyte Activation; D008297:Male; D004676:Myelin Basic Protein / Q000008:administration & dosage / Q000276:immunology; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000008:administration & dosage / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000276:immunology* / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D001323:Autoantibodies; D018984:Epitopes, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; C571326:MOG protein, human; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments