[A nutrient medium for the isolation and cultivation of Campylobacter].
Campylobacter agar, nutrient medium intended for the isolation of bacteria of the genus Campylobacter from clinical material, has been developed. The composition of the medium includes sprat hydrolysate, aerotolerant additive (ferric sulfate--oxide, sodium pyruvate, sodium pyrosulfite), sodium glutaminate, agar. The selective properties of the medium are ensured by introducing the mixture of antibiotics consisting of polymyxin B, rifampicin, amphotericin B, ristomycin. The balanced composition of Campylobacter agar ensures the aerotolerance of Campylobacter organisms and gives the optimal conditions for their growth when the inoculated material is cultivated in the atmosphere made up of the mixture of three gases (5% of oxygen, 10% of carbon dioxide, 85% of nitrogen), as well as under the conditions of a "candle vessel". The medium suppresses the development of the associative microflora diluted 10(-1). As shown in the trial of the quality of Campylobacter agar by the inoculation of material taken from patients with acute enteric infections, agricultural animals and monkeys, the medium has pronounced selective, properties with regard to extraneous microflora, while ensuring the isolation of Campylobacter on the level of the control medium.
Temirkhanova
Z U
ZU
Research and Production Amalgamation Nutrient Media, Makhachkala, Russi
Gashimova
P Sh
PSh
Safonova
N V
NV
Moroz
A F
AF
Khazenson
L V
LV
D000362:Agar; D000704:Analysis of Variance; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D002167:Campylobacter / Q000254:growth & development / Q000302:isolation & purification*; D017000:Campylobacter coli / Q000254:growth & development / Q000302:isolation & purification; D016123:Campylobacter jejuni / Q000254:growth & development / Q000302:isolation & purification; D003470:Culture Media; D006801:Humans
D003470:Culture Media; D000362:Agar
Pitatel'naia sreda dlia vydeleniia i kul'tivirovaniia kampilobakteriĭ.