title: Neurofilament architecture combines structural principles of intermediate filaments with carboxy-terminal extensions increasing in size between triplet proteins.
abstract: Mammalian neurofilament triplet proteins (68 K, 160 K and 200 K) have been correlated by a biochemical, immunological and protein chemical study. The 160 K and 200 K triplet proteins are intermediate filament proteins in their own right, since they reveal the alpha-helical coiled-coil rod domain analyzed in detail for the 68 K protein. Triplet proteins display two distinct arrays. Their amino-terminal region built analogously to non-neuronal intermediate filament proteins should allow a co-polymerization process via the interaction of coiled-coil domains. The extra mass of all triplet proteins is allocated to carboxy-terminally located extensions of increasing size and unique amino acid sequences. These may provide highly charged scaffolds suitable for interactions with other neuronal components. Such a domain of 68 K reveals, in sequence analysis, 47 glutamic acids within 106 residues. The epitope recognized by a monoclonal antibody reacting probably with all intermediate filament proteins has been mapped. It is located within the last 20 residues of the rods, where six distinct intermediate filament proteins point to a consensus sequence.
authors.affiliation: Max-Planck-Institute for Biophysical Chemistry, Göttingen, FRG.
authors.forename: N
authors.initials: N
authors.lastname: Geisler
authors.forename: E
authors.initials: E
authors.lastname: Kaufmann
authors.forename: S
authors.initials: S
authors.lastname: Fischer
authors.forename: U
authors.initials: U
authors.lastname: Plessmann
authors.forename: K
authors.initials: K
authors.lastname: Weber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002918:Chymotrypsin; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D007382:Intermediate Filaments / Q000378:metabolism; D008969:Molecular Sequence Data; D016900:Neurofilament Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D016900:Neurofilament Proteins; D002918:Chymotrypsin