title: Regeneration of halved embryonic lower first mouse molars: correlation with the distribution pattern of non dividing IDE cells, the putative organizers of morphogenetic units, the cusps.
abstract: Recently we demonstrated that non-cycling, cap-stage, mouse molar inner dental epithelial (IDE) cells corresponding to the primary enamel knot (EK) area underwent a coordinated temporo-spatial patterning leading to their patchy irregular segregation at the tips of the forming cusps. These non-cycling cells were suggested to perhaps represent the organizers of the morphogenetic units (OMU), the cusps. The present study has analyzed the regenerative capacity of halved cap-stage first lower mouse molars through three dimensional (3D) reconstructions. Partial regeneration of the anterior half and possible complete regeneration of the posterior half were documented. Using BrdU (5-bromo-2'-deoxyuridine) labeling and 3D reconstructions of the IDE, we have correlated the patterns of cusp regeneration with the distribution of BrdU negative IDE cells. These data support a morphogenetic role for the non-cycling IDE cells.
authors.affiliation: INSERM U-424, Institut de Biologie Médicale, Faculté de Médecine, Strasbourg, France.
authors.forename: R
authors.initials: R
authors.lastname: Coin
authors.forename: R
authors.initials: R
authors.lastname: Schmitt
authors.forename: H
authors.initials: H
authors.lastname: Lesot
authors.forename: J L
authors.initials: JL
authors.lastname: Vonesch
authors.forename: J V
authors.initials: JV
authors.lastname: Ruch
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D003481:Cuspid / Q000196:embryology*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000196:embryology*; D009924:Organ Culture Techniques; D012038:Regeneration; D013997:Time Factors
chemical_list: D001973:Bromodeoxyuridine