A common step for changing cell shape in fruiting body and starvation-independent sporulation of Myxococcus xanthus.
Myxococcus xanthus can sporulate in either of two ways: at the end of the program of fruiting body development or after exposure of growing cells to certain reagents such as concentrated glycerol. Fruiting body sporulation requires starvation, while glycerol sporulation requires rapid growth, and since the two types of spores are structurally somewhat different, it has generally been assumed that the two processes are different. However, a Tn5 Lac insertion mutation, Omega7536, has been isolated which simultaneously blocks the development of fruiting body spores as well as glycerol-induced spores. Both sporulation pathways are blocked in the mutant within the process that converts a rod-shaped cell into a spherical spore. The Omega7536 locus is expressed at the time of cell shape change appropriate to each process, early after glycerol induction and late after starvation induction. On the C-signal response pathway, it is possible to identify positions for the normal function of the Omega7536 locus and for the inducing stimulus from glycerol that are unique and consistent with the observations. Although the two sporulation pathways differ in certain respects, it is shown that they share at least one step for changing a rod-shaped cell into a spherical spore.
Licking
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Departments of Biochemistry and Developmental Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Gorski
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Kaiser
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D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D005990:Glycerol / Q000378:metabolism; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D016254:Mutagenesis, Insertional; D016941:Myxococcus xanthus / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D013171:Spores, Bacterial / Q000502:physiology; D001616:beta-Galactosidase
D001426:Bacterial Proteins; C063576:CsgA protein, Myxococcus xanthus; D004251:DNA Transposable Elements; D001616:beta-Galactosidase; D005990:Glycerol