[The use of chemiluminescence for assessing the physiological status of macrophages activated by pertussis preparations].
The work deals with the comparative analysis of oxygen-dependent metabolic processes in the peritoneal macrophages of intact and immune, mice after their interaction with whole-cell pertussis vaccine (WCPV), dialyzed pertussis antigen (DPA) and fraction 2 of this antigen. The study revealed that WCPV, DPA and its fraction 2 modulated the production of active forms of oxygen by peritoneal macrophages, evaluated by means of luminol-dependent chemiluminescence (CL). The influence of WCPV of oxygen-dependent metabolic processes in macrophages after the first contacts with them had a dose-dependent character: low concentrations activated and higher concentrations suppressed the "respiratory" explosion. The immunization of animals abolished the effect of extinguishing CL on the contact of macrophages with high doses of WCPV and essentially increased the level of their CL response to all pertussis preparations in comparison with those for intact cells. DPA and its fraction 2 were not inferior to WCPV in their capacity for inducing the "respiratory" explosion, and in high doses they essentially surpassed WCPV doses in this capacity, especially on the first contact with macrophages.
Moskalenko
E P
EP
State Medical University, Rostov-on-Don, Russia.
Tiukavkina
S Iu
SIu
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000494:pharmacology*; D001886:Bordetella pertussis / Q000276:immunology*; D016001:Confidence Intervals; D004306:Dose-Response Relationship, Immunologic; D007114:Immunization; D008163:Luminescent Measurements; D008262:Macrophage Activation / Q000187:drug effects* / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010567:Pertussis Vaccine / Q000276:immunology / Q000494:pharmacology*; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology
D000942:Antigens, Bacterial; D010567:Pertussis Vaccine
Ispol'zovanie khemiliuminestsentsii dlia otsenki fiziologicheskogo sostoianiia makrofagov, aktivirovannykh kokliushnymi preparatami.