title: Estradiol-17beta sulfotransferase activity in canine osteosarcoma D17 cells.
abstract: Estrogen sulfatase and sulfotransferase (EST) activities are present in breast cancer tissues but there are no reports on EST in cancerous bone cells. We incubated [(3)H]estradiol-17beta with cells from a canine osteosarcoma D17 line for periods up to 24 h. Radioactive steroids were recovered from the media and separated into unconjugated and conjugated fractions using Sep-Pak C18 cartridges. The conjugate fraction was solvolyzed and the resulting free steroids were obtained from a second C18 cartridge. Little metabolism was apparent in 4 h of incubation, but by 24 h as much as one half of the radioactivity was seen in the conjugate fraction. Most of the conjugates were recovered as sulfates in all three experiments. HPLC profiles showed a limited metabolism of estradiol to other compounds except for estrone, which was clearly present in both free and sulfate fractions. These results suggest that EST may have a role in the local metabolism of estrogens in bone.
authors.affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: J I
authors.initials: JI
authors.lastname: Raeside
authors.forename: H L
authors.initials: HL
authors.lastname: Christie
authors.forename: L
authors.initials: L
authors.lastname: Forster
authors.forename: R L
authors.initials: RL
authors.lastname: Renaud
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000201:enzymology*; D001842:Bone and Bones / Q000378:metabolism; D001943:Breast Neoplasms / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006801:Humans; D012516:Osteosarcoma / Q000201:enzymology*; D013429:Sulfatases / Q000378:metabolism; D015238:Sulfotransferases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D004958:Estradiol; D015238:Sulfotransferases; C037564:estradiol-17 beta sulfotransferase; D013429:Sulfatases; C039596:estrogen sulfatase