The application of single cell gel electrophoresis or Comet assay to human monitoring studies.
OBJECTIVE
In the search of new human genotoxic biomarkers, the single cell gel electrophoresis assay has been proposed as a sensible alternative.


METHODS
This technique detects principally single strand breaks as well as alkali-labile and repair-retarded sites.


RESULTS
Herein we present our experience using the single cell gel electrophoresis assay in human population studies, both occupationally and environmentally exposed.


CONCLUSIONS
We discuss the assay feasibility as a genotoxic biomarker.
Valverde
M
M
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM).
Ostrosky-Wegman
P
P
Rojas
E
E
Fortoul
T
T
Meneses
F
F
Ramírez
M
M
Díaz-Barriga
F
F
Cebrian
M
M
D015415:Biomarkers / Q000032:analysis; D020552:Comet Assay; D004781:Environmental Exposure; D006801:Humans; D008800:Mexico; D008991:Monitoring, Physiologic; D016273:Occupational Exposure; D014505:Urban Population
D015415:Biomarkers