Characterization of a C-terminal-type kinesin-related protein from Dictyostelium discoideum.
We have determined the full sequence of K2, a kinesin-related protein (KRP) in Dictyostelium discoideum. Sequence homology and domain organization placed K2 in the ncd/Kar3 subfamily of the C-terminal-type KRPs. Bacterially expressed, truncated K2 showed ATP-dependent binding to microtubules and microtubule-stimulated ATPase activity. K2-null cells grew and developed normally, suggesting overlapping functions of K2 with other microtubule motor(s). Overexpression of K2 caused partial mitotic arrest. Green fluorescent protein-tagged full-length K2 localized in the nucleus at the interphase and on the mitotic spindle during mitosis. These results suggest that K2 is a microtubule-dependent motor which may play some roles in mitotic spindles.
Iwai
S
S
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153-8902, Tokyo, Japan.
Suyama
E
E
Adachi
H
H
Sutoh
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D004023:Dictyostelium; D016547:Kinesins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D002135:Calcium-Binding Proteins; D009124:Muscle Proteins; C074152:kinesin-II; D016547:Kinesins