Activity of neurons in the nucleus of the solitary tract of rats: effect of osmotic and mechanical stimuli.
Patch-clamp recordings were used to examine the osmosensitivity and mechanosensitivity of neurons in the caudal part of the nucleus tractus solitarius in coronal slices from rat brain. Firing rates and membrane potentials were measured as slices were exposed to perfusate which varied in its osmolality and/or sodium concentration. In all cells tested, the responses to change in the sodium concentration of perfusate were duplicated by osmolality changes of sucrose or mannitol. When nucleus tractus solitarius cells were tested with changes in pressure applied via the pipette, responses to positive or negative pressure paralleled their responses to osmotic stimulation. We suggest that a mechanosensitive receptor exists on osmosensitive neurons within the nucleus tractus solitarius, and this receptor may be responsible for changes in the firing rate and membrane potential which occur in the nucleus tractus solitarius neurons.
Izawa
S
S
Department of Operative Dentistry, Okayama University Dental School, Japan.
Inoue
K
K
Adachi
A
A
Funahashi
M
M
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D004234:Diuretics, Osmotic / Q000494:pharmacology; D006982:Hypertonic Solutions / Q000494:pharmacology; D007038:Hypotonic Solutions / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology; D017552:Solitary Nucleus / Q000166:cytology* / Q000502:physiology*; D013268:Stimulation, Chemical; D013395:Sucrose / Q000494:pharmacology; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology*
D004234:Diuretics, Osmotic; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D008353:Mannitol; D012965:Sodium Chloride; D013395:Sucrose