title: Use of short high-performance liquid chromatography columns and tandem-mass spectrometry for the rapid analysis of a prostaglandin analog, fluprostenol, in rat plasma.
abstract: A short reversed-phase HPLC column and a tandem mass spectrometer were used to develop a stable-isotope-dilution assay for the rapid and sensitive analysis of fluprostenol, a prostaglandin analog, in rat plasma. A Waters Symmetry ODS column (2.1x10 mm) afforded rapid isocratic elution of fluprostenol (t(R)=40 s) but still provided a relatively large k' value of 4. The use of tandem mass spectrometry allowed the interference-free detection of fluprostenol under the rapid elution conditions, with a limit of quantitation of 25 pg ml(-1) fluprostenol, using 0.2 ml plasma sample volumes. The method was linear over three orders of magnitude, yielded accurate and precise results and allowed the pharmacokinetic profile of fluprostenol to be defined following intravenous administration in rats.
authors.affiliation: Procter & Gamble Pharmaceuticals, Mason, OH 45040, USA.
authors.forename: T H
authors.initials: TH
authors.lastname: Eichhold
authors.forename: D L
authors.initials: DL
authors.lastname: Kuhlenbeck
authors.forename: T R
authors.initials: TR
authors.lastname: Baker
authors.forename: M E
authors.initials: ME
authors.lastname: Stella
authors.forename: J S
authors.initials: JS
authors.lastname: Amburgey
authors.forename: M A
authors.initials: MA
authors.lastname: deLong
authors.forename: J R
authors.initials: JR
authors.lastname: Hartke
authors.forename: C A
authors.initials: CA
authors.lastname: Cruze
authors.forename: S A
authors.initials: SA
authors.lastname: Pierce
authors.forename: K R
authors.initials: KR
authors.lastname: Wehmeyer
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry; D011461:Prostaglandins F, Synthetic / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D011461:Prostaglandins F, Synthetic; C006326:fluprostenol