[Disordered differentiation of mouse embryonic lungs in organ cultures under the action of nitrosomethylurea].
We have studied the effect of nitrosomethylurea (NMU) on differentiation of early rudiments of mouse embryonic lungs (12th day of embryogenesis) explanted into an organ culture. We have demonstrated that nontoxic doses of NMU are capable of accelerating normal lung differentiation both at the early (increase in the number of epithelial buds) and at the late (increase in the number of explants with regions of well-developed alveoli) stages of cultivation. However, NMU induces disturbances of differentiation, which appear as polycystic structures and hyperplastic nodules generally absent in the control.
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Institute of Carcinogenesis, Blokhin Oncological Science Center, Russian Academy of Medical Sciences, Moscow, Russia.
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D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005865:Gestational Age; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000196:embryology* / Q000378:metabolism; D008770:Methylnitrosourea / Q000494:pharmacology*; D051379:Mice; D009924:Organ Culture Techniques; D013997:Time Factors
D000477:Alkylating Agents; D008770:Methylnitrosourea
Narushenie differentsirovki émbrional'nykh legkikh mysheĭ v organnykh kul'turakh pod deĭstviem nitrozometilmocheviny.