Determination of histamine in tomatoes by liquid chromatography/mass spectrometry.
The histamine level in tomato fruits and pastes was determined by 2 orthogonal techniques as a means of comparing accuracy. Close statistical agreement was found between assays for free histamine by capillary electrophoresis (with UV absorbance detection), and for the dansyl derivative by reversed-phase liquid chromatography (LC). Both techniques have adequate sensitivity for the analysis of endogenous histamine in tomatoes, but LC/electrospray tandem mass spectrometry was more sensitive by at least an order of magnitude, down to a level of 0.05 mg/kg.
Bolygo
E
E
Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire, UK.
Cooper
P A
PA
Jessop
K M
KM
Moffatt
F
F
D002853:Chromatography, Liquid; D006632:Histamine / Q000032:analysis*; D018551:Solanum lycopersicum / Q000737:chemistry*; D013058:Mass Spectrometry; D008956:Models, Chemical
D006632:Histamine