title: Enantioselective determination of bromoisovalerylurea by liquid chromatography on chiral stationary phase in reversed- or normal-phase partition mode.
abstract: Bromoisovalerylurea (bromvalerylurea) is a sedative-hypnotic given orally as a racemate. Enantiomers of this drug could be separated by high-performance liquid chromatography on the three chiral stationary phases (a vancomycin-bonded, beta-cyclodextrin derivative-bonded, or urea derivative-bonded phase). Biological fluids of human subjects who had ingested toxic or therapeutic doses of the racemate were chromatographed after liquid-liquid extraction. The (+)-enantiomer concentration was almost equal to the (-)-enantiomer concentration in the serum of one overdosed patient. In all the other subjects, the (+)-enantiomer was less than the (-)-enantiomer in their sera and saliva. The data suggest that the drug is absorbed non-stereoselectively from the gastrointestinal tract and eliminated from the blood stereoselectively.
authors.affiliation: Department of Clinical Pathology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa, Japan. nishika@kitasato-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Nishikawa
authors.forename: H
authors.initials: H
authors.lastname: Ohtani
authors.forename: Y
authors.initials: Y
authors.lastname: Kamijo
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtani
authors.forename: R
authors.initials: R
authors.lastname: Kondo
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Okuda
mesh_terms: D000328:Adult; D001968:Bromisovalum / Q000032:analysis* / Q000097:blood / Q000506:poisoning; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D062787:Drug Overdose / Q000097:blood; D006801:Humans; D006993:Hypnotics and Sedatives / Q000032:analysis* / Q000097:blood / Q000506:poisoning; D008297:Male; D012463:Saliva / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D006993:Hypnotics and Sedatives; D001968:Bromisovalum