title: Adsorption of inulinases in ion-exchange columns.
abstract: The use of adsorption columns packed with ion-exchange resins for recovering, concentrating and purifying proteins is now widespread. The present work consists of a study on the dynamic behavior of adsorption columns that uses two kinds of adsorbents: a cationic and an anionic resin. A frontal analysis of the columns was performed with experimental data obtained from Fructozyme, a mixture of inulinase enzymes. The parameters of a Langmuir type of isotherm and adsorption kinetics were obtained from experimental tests in a batch system. A numerical technique based on orthogonal collocation and a fourth-order Runge-Kutta method was coupled with a nonlinear optimization method to predict the coefficients of the rate equations, which are fundamental for scale-up purposes.
authors.affiliation: School of Chemical Engineering, Universidade Estadual de Campinas, UNICAMP, SP, Brazil.
authors.forename: F R
authors.initials: FR
authors.lastname: Silva
authors.forename: C C
authors.initials: CC
authors.lastname: Santana
mesh_terms: D000327:Adsorption; D001230:Aspergillus / Q000201:enzymology; D001234:Aspergillus niger / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D002912:Chrysosporium / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D007475:Ion Exchange Resins; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology; D012685:Sepharose / Q000031:analogs & derivatives
chemical_list: D007475:Ion Exchange Resins; C047264:sepharose CL 6B; D012685:Sepharose; D006026:Glycoside Hydrolases; C023086:inulinase