title: The fibrous structure of the cemento-dentinal junction in human molars shown by scanning electron microscopy combined with NaOH-maceration.
abstract: The cemento dentinal junction was studied in acellular and cellular cementum of human mandibular third molars by scanning electron microscopy combined with NaOH-maceration. Scanning electron microscopy with NaOH-maceration was applied to observe the fibrous structure in detail through long sections of the cemento-dentinal junction. In macerated specimens, the cemento dentinal junction was a fibril-poor groove. Some cemental fibrils or fibril bundles penetrated the groove and appeared to intermingle with dentinal fibrils. Prolonged maceration caused detachment of the cemento-dentinal junction irrespective of fibril intermingling allowing observation of the inner cementum surface facing the root dentin. Observations suggested that the fibril intermingling was point-like and present only in places at the cemento-dentinal junction. It was established that NaOH-maceration removes interfibrillar substances effectively in connective tissues and does no damage to the collagen fibril structure and architecture. This study showed the 3-dimensional fibrous structure of the cemento-dentinal junction in human mandibular third molars, and suggested that interfibrillar adhesive substances are more important than the fibril intermingling for the cemento-dentinal attachment.
authors.affiliation: Department of Oral Anatomy II, Hokkaido University School of Dentistry, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Domon
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: M N
authors.initials: MN
authors.lastname: Islam
authors.forename: R
authors.initials: R
authors.lastname: Suzuki
mesh_terms: D002424:Caustics; D003739:Dental Cementum / Q000648:ultrastructure*; D003804:Dentin / Q000648:ultrastructure*; D016591:Histocytological Preparation Techniques; D006801:Humans; D008334:Mandible; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008964:Molar, Third / Q000648:ultrastructure*; D012972:Sodium Hydroxide
chemical_list: D002424:Caustics; D012972:Sodium Hydroxide