Y-chromosomal STR haplotypes in a population sample from Bavaria.
The seven Y-chromosomal STRs DYS 19, DYS385 I/II, DYS389 I/II, DYS390, DYS391, DYS392 and DYS393 were amplified using two multiplex PCRs. The optimization of the PCR conditions led to reliable and sensitive systems. Co-amplification of the amelogenin locus was possible in both multiplex systems. In a population sample of 151 Bavarian males, a gene diversity of 0.99 was observed. Sensitivity studies revealed a detection limit of 50 pg DNA per 25 microl reaction volume. PCR experiments with combinations of male/male and male/female DNA showed that in male/male mixtures, the minor component could be detected up to a ratio of 1:15, whereas in male/female mixtures the male component could be found in a higher ratio up to 1:60.
Anslinger
K
K
Institute of Legal Medicine, Ludwig-Maximilians-University of Munich, Germany.
Keil
W
W
Weichhold
G
G
Eisenmenger
W
W
D001801:Blood Stains; D016208:Databases, Factual; D005260:Female; D005554:Forensic Medicine; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics*; D005858:Germany; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012727:Sex Characteristics; D014998:Y Chromosome / Q000235:genetics*
D005819:Genetic Markers