[Effect of gangliosides on intensity of the lipid peroxidation process and structural changes in neuronal membranes, caused by toxic doses of glutamate].
We studied effects of gangliosides on the level of lipid peroxides and microviscosity of membrane lipid bilayer in primary dissociated cultures of cerebellar granule cells prepared from 8 day-old rats under conditions of neurotoxic effect of glutamate. It was found that glutamate (100 mkM) treatment of primary cultures activated the processes of lipid peroxidation and decreased microviscosity of neuronal membranes determined as a degree of pyrene excimerization. It was also shown that preincubation of granule cells with gangliosides did not prevent the accumulation of TBA-reactive products induced by glutamate. At the same time gangliosides significantly decreased the membrane-fluidizing effect caused by glutamate.
Ivanova
V P
VP
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg.
Andreeva
N A
NA
Dupin
A M
AM
Erin
A N
AN
Viktorov
I V
IV
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005732:Gangliosides / Q000494:pharmacology*; D018698:Glutamic Acid / Q000633:toxicity*; D008051:Lipid Bilayers; D015227:Lipid Peroxidation / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005732:Gangliosides; D008051:Lipid Bilayers; D017392:Thiobarbituric Acid Reactive Substances; D018698:Glutamic Acid
Vliianie gangliozidov na intensivnost' protsessov perekisnogo okisleniia lipidov i strukturnye izmeneniia neĭronal'noĭ membrany, vyzvannye toksicheskimi dozami glutamata.