title: The budding yeast homolog of the human EBNA1-binding protein 2 (Ebp2p) is an essential nucleolar protein required for pre-rRNA processing.
abstract: The human EBP2 protein was found by two-hybrid analysis to interact with the Epstein-Barr virus nuclear antigen 1 (EBNA1). Homologs of human EBP2 can be found in Caenorhabditis elegans, Schizosaccharomyces pombe, and in Saccharomyces cerevisiae, and they all share a conserved 200-300-amino acid block of residues at their C termini. To understand the cellular function of EBP2, we have begun to study the protein in S. cerevisiae. The yeast Ebp2 protein contains N-terminal, nucleolar-associated KKE motifs, and deletion analysis reveals that the C-terminal conserved region is required for the activity of the protein. The EBP2 gene codes for an essential protein that localizes to the nucleolus. Temperature-sensitive ebp2-1 mutants become depleted of ribosomes and cease to divide after several generations at the restrictive temperature of 36 degrees C. This decline in ribosome levels is accompanied by a diminution in the levels of the 35 S-derived recombinant RNAs (rRNAs) (in particular the 25 S and 5.8 S rRNAs). Pulse-chase, Northern, and primer extension analysis of the rRNA biosynthetic pathway indicates that ebp2-1 mutants are defective in processing the 27 SA precursor into the 27 SB pre-rRNA.
authors.affiliation: Molecular Biology and Biochemistry Department, Wesleyan University, Middletown, Connecticut 06459-0175, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Huber
authors.forename: J H
authors.initials: JH
authors.lastname: Dworet
authors.forename: K
authors.initials: K
authors.lastname: Shire
authors.forename: L
authors.initials: L
authors.lastname: Frappier
authors.forename: M A
authors.initials: MA
authors.lastname: McAlear
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002466:Cell Nucleolus / Q000737:chemistry*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000378:metabolism*; D005656:Fungal Proteins / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D005656:Fungal Proteins; D012322:RNA Precursors; D012335:RNA, Ribosomal