title: Lymphokine release from human lymphomononuclear cells after co-culture with isolated pancreatic islets: effects of islet species, long-term culture, and monocyte-macrophage cell removal.
abstract: This study evaluated the release of Th1 and Th2 cytokines from human lymphomononuclear cells (LMC) in response to purified human (HI) or bovine (BI) islets, and the role of long-term (3-4 weeks) islet culture and removal of monocyte-macrophage cells. The results showed that HI and BI caused a similar increase of the release of gamma interferon (IFN), IL-2 and IL-6 from LMC, whereas BI had a more marked effect than HI on IL-10 release. Culturing the islets had possible positive effects (reduction of IFN and IL-2), but also potentially negative effects (increase of TNF). Removal of monocyte-macrophage cells determined a significant reduction of IL-6, IL-10 and TNF production in response to xeno-islets.
authors.affiliation: Dipartimento di Endocrinologia e Metabolismo, University of Pisa, Italy. metabpri@immr.med.unipi.it
authors.forename: L
authors.initials: L
authors.lastname: Marselli
authors.forename: P
authors.initials: P
authors.lastname: Marchetti
authors.forename: C
authors.initials: C
authors.lastname: Tellini
authors.forename: R
authors.initials: R
authors.lastname: Giannarelli
authors.forename: C
authors.initials: C
authors.lastname: Lencioni
authors.forename: S
authors.initials: S
authors.lastname: Del Guerra
authors.forename: R
authors.initials: R
authors.lastname: Lupi
authors.forename: M
authors.initials: M
authors.lastname: Carmellini
authors.forename: F
authors.initials: F
authors.lastname: Mosca
authors.forename: R
authors.initials: R
authors.lastname: Navalesi
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D007376:Interleukin-2; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007372:Interferons