title: Chromatin remodelling during the life cycle of trypanosomatids.
abstract: The mechanisms which control the expression of developmentally regulated genes in trypanosomatids remain unclear. The genes are grouped together into transcription units that are co-transcribed to yield polycistronic RNAs. Trans-splicing and polyadenylation give rise to mature, monocistronic mRNAs. It is difficult to imagine that expression of these genes is controlled at the level of transcription initiation because this would suggest that the genes are transcribed at the same rate. This is not the case, because at any given developmental stage in trypanosomes or Leishmania, genes transcribed from the same transcription unit are expressed at different levels within the cell. Consequently, these parasites must rely on post-transcriptional or post-translational mechanisms to generate the appropriate levels of gene product within the cell. There are no well-established examples of RNA polymerase II promoters in trypanosomes or Leishmania. However, the promoters for genes encoding the variant surface glycoprotein (VSG) and the procyclic acidic repetitive protein (PARP) have been identified and resemble ribosomal RNA polymerase I promoters. In higher eukaryotes where the mechanisms regulating transcription are clearer, there is increasing evidence that epigenetic factors, such as histones and modified bases, influence gene expression. Chemical modification of these factors can restructure chromatin and lead to gene activation or silencing. In trypanosomatids, an epigenetic mechanism for the control of developmentally expressed genes is a possibility. In this review, chromatin remodelling during the life and cell cycle of trypanosomes and Leishmania is explored, and the influence of epigenetic factors such as histones and modified bases on this process is discussed.
authors.affiliation: Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology, Sydney, Westbourne Street, Gore Hill, NSW 2065, Australia. Sabina.Belli@uts.edu.au
authors.forename: S I
authors.initials: SI
authors.lastname: Belli
mesh_terms: D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D005838:Genotype; D006657:Histones / Q000737:chemistry; D007621:Karyotyping; D008018:Life Cycle Stages; D015533:Transcriptional Activation; D014345:Trypanosoma / Q000235:genetics / Q000254:growth & development*
chemical_list: D002843:Chromatin; D006657:Histones