In vitro study of the effects of endothelin-1 on human dental pulp cells.
OBJECTIVE
To study the effect of Endothelin-1 (ET-1) on cell proliferation in primary cultures of human dental pulp cells; the cell mechanism of mitogen; and collagen synthesis.


METHODS
Primary cultures of human dental pulp cells; Thiazolyl Blue Tetrazolium Bromide (MTT) test to investigate cell proliferation; measurement of calcium ions in human dental pulp cells with the calcium-sensitive dye fluo-3; enzyme-linked immunosorbent assay and avidin-biotin complex immunocytochemical assay for collagen synthesis in human dental pulp cells.


RESULTS
Incubation of cultures with ET-1 resulted in a dose-dependent increase in cell proliferation over control (unstimulated) cultures. In addition ET-1 increased the Ca2+ concentration in human dental pulp cells, which cannot be inhabited by Verapamil. The finding demonstrated that collagen type I synthesis of human dental pulp cells was increased by ET-1.


CONCLUSIONS
ET-1 played a crucial role in pulp restoration of lesions and had an effect on mitogen and differentiation of human dental pulp cells.
Yan
Y
Y
Department of Oral Pediatrics, School of Stomatology, Shanghai Second Medical University, P. R. China.
Liu
Z
Z
Zhang
W G
WG
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects*; D019332:Endothelin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D014665:Vasodilator Agents / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D019332:Endothelin-1; D014665:Vasodilator Agents; D014700:Verapamil