Cutting edge: antilisterial activity of CD8+ T cells derived from TNF-deficient and TNF/perforin double-deficient mice.
The mechanisms by which CD8+ T cells mediate immunity against bacterial pathogens remain largely unknown. Perforin-dependent cytolysis plays a role, but is not required for CD8+ T cell-mediated immunity against Listeria monocytogenes. TNF is essential for CD8+ T cell immunity to L. monocytogenes, but the cellular source of TNF is undefined. TNF-deficient and TNF/perforin double-deficient mice were used to generate CD8+ T cells specific for an L. monocytogenes-derived Ag. Wild-type and TNF-deficient CD8+ T cells mediated antilisterial immunity in wild-type but not TNF-deficient host mice, revealing that CD8+ T cell-derived TNF is not required for CD8+ T cell-mediated antilisterial immunity, but demonstrating a role for TNF derived from other cell types. TNF/perforin double-deficient CD8+ T cells mediated antilisterial immunity in the liver, but not in the spleen, of wild-type recipient mice, suggesting that perforin-independent immunity in the spleen requires CD8+ T cell-derived TNF.
White
D W
DW
Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City 52242, USA.
Badovinac
V P
VP
Kollias
G
G
Harty
J T
JT
D019264:Adoptive Transfer; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000382:microbiology* / Q000637:transplantation; D007113:Immunity, Innate / Q000235:genetics; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D008088:Listeriosis / Q000235:genetics / Q000276:immunology; D008099:Liver / Q000276:immunology / Q000382:microbiology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000172:deficiency* / Q000235:genetics*; D014774:Virulence
D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D014409:Tumor Necrosis Factor-alpha; D054353:Perforin; C072980:actA protein, Listeria monocytogenes