PCR amplification and phylogenetic analysis of groESL operon sequences from Ehrlichia ewingii and Ehrlichia muris.
Broad-range PCR primers were used to amplify part of the groESL operon of the canine pathogen Ehrlichia ewingii, recently recognized as a human pathogen, and the murine pathogen Ehrlichia muris. Phylogenetic analysis supported the relationships among Ehrlichia species previously determined by comparison of 16S rRNA gene sequences. These sequences provide additional PCR targets for species for which few gene sequences have been determined.
Sumner
J W
JW
Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jws3@cdc.gov
Storch
G A
GA
Buller
R S
RS
Liddell
A M
AM
Stockham
S L
SL
Rikihisa
Y
Y
Messenger
S
S
Paddock
C D
CD
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002478:Cells, Cultured; D018833:Chaperonins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs; D004536:Ehrlichia / Q000145:classification* / Q000235:genetics; D016873:Ehrlichiosis / Q000382:microbiology*; D020459:Genes, rRNA; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009876:Operon; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C097970:GroESL protein, Bacteria; D012336:RNA, Ribosomal, 16S; D018833:Chaperonins