Neutralizing potency of horse antibothropic Brazilian antivenom against Bothrops snake venoms from the Amazonian rain forest.
Neutralization of lethal toxicity (50% effective dose; ED(50)), hemorrhagic (minimum hemorrhagic dose; MHD) and hemolytic activity (PLA(2)) and levels of antibodies, measured by enzyme-linked immunosorbent assay (ELISA), were investigated to test the potency of horse antibothropic serum (ABS) against Bothrops venoms from the Amazonian rain forest. ABS neutralized the lethal activity with a potency (mg of venom neutralized per 1 ml of antivenom) of 5.5, 3.7, 1.6, 1.3 and 6.5, respectively, for B. jararaca (reference venom for assessing the ABS potency in Brazil), B. atrox, B. brazili, B. bilineatus smaragdinus and B. taeniatus venoms. The volume of antivenom (microl) that neutralized one MHD of B. jararaca, B. atrox, B. brazili, B. bilineatus smaragdinus and B. taeniatus venoms was 5, 7.71, 7.76, 8.3 and 5, respectively. ABS neutralized the PLA(2) activity with a potency of 6.2, 3.2, 1.4, 2.6 and 5 respectively, for B. jararaca, B. atrox, B. brazili, B. bilineatus smaragdinus and B. taeniatus venoms. ELISA reactivity of ABS against the separate venoms was found to be quite variable. The reactivity against B. jararaca venom was higher than against other Bothrops venoms. In conclusion, the assays described here suggest that Brazilian Bothrops polyspecific antivenom is not very efficient in neutralizing the effects of venom from some Amazonian Bothrops species.
Muniz
E G
EG
Gerência de Animais Peçonhentos, Fundação de Medicina Tropical, Instituto de Medicina Tropical do Amazonas, AM, Manaus, Brazil.
Maria
W S
WS
Estevão-Costa
M I
MI
Buhrnheim
P
P
Chávez-Olórtegui
C
C
D000818:Animals; D000997:Antivenins / Q000276:immunology* / Q000494:pharmacology; D017837:Bothrops / Q000145:classification / Q000276:immunology*; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000276:immunology* / Q000633:toxicity; D004791:Enzyme Inhibitors / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006461:Hemolysis / Q000187:drug effects; D006736:Horses / Q000276:immunology*; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D013045:Species Specificity
D000997:Antivenins; D003435:Crotalid Venoms; D004791:Enzyme Inhibitors; D010741:Phospholipases A