title: Bacterial contamination of anterior chamber during IOL surgery.
abstract: OBJECTIVE
  To study the nature and frequency of bacterial contamination during cataract surgery.
  
  
  METHODS
  The preoperative smears from the conjunctiva and anterior chamber (AC) fluid aspirates during extra-capsular cataract surgery (ECCE) with posterior chamber intraocular lens (PCIOL) implantation in 40 eyes were analysed for aerobic and anaerobic bacteria. Any change in the bacterial strains isolated before and after cataract surgery was also studied.
  
  
  RESULTS
  AC fluid aspirates were positive for bacteria in 15 eyes (37.5%). Coagulase-negative Staphylococcus was the most common aerobe (39.4%) and Propionibacterium acnes the most common anaerobe. Of the 15 cases with positive AC fluid cultures, 6 showed an organism in the AC aspirate different from the conjunctival smear.
  
  
  CONCLUSIONS
  Clinically there was no endophthalmitis in any of the eyes. Factors such as preoperative antibiotic use, the antibacterial properties of aqueous, or low inoculum size could explain this. The preoperative conjunctival smear may not be useful in predicting the AC fluid contamination or outcome of cataract surgery.
authors.affiliation: Department of Ophthalmology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India.
authors.forename: R
authors.initials: R
authors.lastname: Srinivasan
authors.forename: R A
authors.initials: RA
authors.lastname: Reddy
authors.forename: S
authors.initials: S
authors.lastname: Rene
authors.forename: R
authors.initials: R
authors.lastname: Kanungo
authors.forename: M K
authors.initials: MK
authors.lastname: Natarajan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000867:Anterior Chamber / Q000382:microbiology*; D000900:Anti-Bacterial Agents; D001419:Bacteria / Q000302:isolation & purification*; D002387:Cataract Extraction / Q000009:adverse effects*; D003228:Conjunctiva / Q000382:microbiology; D004359:Drug Therapy, Combination / Q000627:therapeutic use; D015818:Eye Infections, Bacterial / Q000188:drug therapy / Q000382:microbiology*; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D006801:Humans; D019654:Lens Implantation, Intraocular / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013530:Surgical Wound Infection / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D005938:Glucocorticoids