New specificity and yield enhancer of polymerase chain reactions.
Tetramethylammonium (TMA) chloride, dimethyl sulfoxide and formamide are known to increase, under certain conditions, the specificity and efficiency of the polymerase chain reaction (PCR). We compared the ability of several TMA derivatives and some other reagents to increase the specificity of PCR and to improve the yield of amplification. A novel combination of the enhancer TMA and oxalate as anion is demonstrated to be a powerful enhancer of PCR. Addition of 2 mM TMA oxalate to the PCR mixture decreases the formation of non-specific DNA fragments and increases the yield of specific PCR products.
Kovárová
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Department of Mammalian Gene Expression, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Vídenská 1083, 142 20 Prague 4, Czech Republic.
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D000818:Animals; D007202:Indicators and Reagents; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D051381:Rats; D012680:Sensitivity and Specificity; D018800:Thy-1 Antigens / Q000235:genetics
D007202:Indicators and Reagents; D000644:Quaternary Ammonium Compounds; D018800:Thy-1 Antigens; C027917:tetramethylammonium