title: Distribution of non-collagenous dentin matrix proteins and proteoglycans, and their relation to calcium accumulation in bisphosphonate-affected rat incisors.
abstract: It has been reported that multiple injections of 1-hydroxyethylidene- 1,1-bisphosphonate (HEBP) to rats prevent mineralization of incisor dentin, thereby revealing high concentrations of calcium in the non-mineralized matrix of circumpulpal dentin. To identify the molecules responsible for calcium accumulation in circumpulpal dentin matrix, rats were injected daily with HEBP (8 mg P/kg) for 7 d, and the incisors processed for various histochemical and immunohistochemical staining of non-collagenous matrices of dentin. Cuprolinic blue reactions for proteoglycans (PGs) were equally distributed in non-mineralized matrix of mantle and circumpulpal dentin layers. Dentin sialoprotein (DSP) and osteopontin (OPN) immunoreactions were found in non-mineralized circumpulpal dentin matrix, but not in mantle dentin. In normal incisors, however, predentin matrix showing significant DSP immunoreactivity was negative for Ca-GBHA reactions. HEBP-affected, non-mineralized OPN immunopositive bone matrix was also non-reactive for calcium. From these observations, neither PGs, OPN nor DSP appear to be responsible for calcium accumulation in HEBP-affected circumpulpal dentin. Stains-all reactive component, possibly dentin phosphoprotein (DPP), only showed the same distribution as that of Ca-GBHA in both HEBP-affected and normal dentin matrix, implicating a possible contribution of DPP to calcium accumulation in circumpulpal dentin and, hence, to appositional mineralization of dentin.
authors.affiliation: Department of Pediatric Dentistry, Faculty of Dentistry, Tokyo Medical and Dental University, Japan. oma.pedo@dent.tmd.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Ohma
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
authors.forename: Y
authors.initials: Y
authors.lastname: Takano
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003804:Dentin / Q000737:chemistry* / Q000187:drug effects; D012968:Etidronic Acid / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry* / Q000187:drug effects; D016326:Extracellular Matrix Proteins / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D010750:Phosphoproteins / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000032:analysis; D013194:Staining and Labeling; D014074:Tooth Calcification / Q000187:drug effects* / Q000502:physiology
chemical_list: D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011509:Proteoglycans; D012795:Sialoglycoproteins; D012968:Etidronic Acid; D002118:Calcium