title: Identification of Ehrlichia chaffeensis by nested PCR in ticks from Southern China.
abstract: A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55. 2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.
authors.affiliation: Institute of Microbiology and Epidemiology, Beijing 100071, China. caowc@nic.bmi.ac.cn
authors.forename: W C
authors.initials: WC
authors.lastname: Cao
authors.forename: Y M
authors.initials: YM
authors.lastname: Gao
authors.forename: P H
authors.initials: PH
authors.lastname: Zhang
authors.forename: X T
authors.initials: XT
authors.lastname: Zhang
authors.forename: Q H
authors.initials: QH
authors.lastname: Dai
authors.forename: J S
authors.initials: JS
authors.lastname: Dumler
authors.forename: L Q
authors.initials: LQ
authors.lastname: Fang
authors.forename: H
authors.initials: H
authors.lastname: Yang
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D002681:China; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018469:Ehrlichia chaffeensis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009758:Nymph / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial