Calmodulin gene expression in an immortalized striatal gabaergic cell line.
We have demonstrated the presence of the mRNAs transcribed from the three calmodulin (CaM) genes in the GABAergic cell line M26-1F derived from embryonic rat striatal cells and immortalized by oncogene transduction. Similarly as in the rat striatum in vivo, these clonal cells express CaM I, CaM II and CaM III mRNAs differently, the CaM I mRNA population being the most abundant, followed in sequence by the CaM II and CaM III mRNA populations. The proportions of these transcripts resemble those in the adult striatum. The possibility of deriving immortalized cell lines from primary neuronal tissue which exhibit characteristics similar to those of the tissue of origin could provide an important tool in many types of in vitro studies.
Pálfi
A
A
Department of Zoology and Cell Biology, University of Szeged, Hungary.
Tarcsa
M
M
Várszegi
S
S
Gulya
K
K
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D002147:Calmodulin / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002147:Calmodulin; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D005680:gamma-Aminobutyric Acid