title: The metabotropic glutamate receptor subtype mGluR 2/3 is located at extrasynaptic loci in rat spinal dorsal horn synapses.
abstract: The position of neurotransmitter receptors relative to active neurotransmitter release sites may be a major factor influencing neuronal responses. The location of the metabotropic glutamate receptor subtype mGluR2/3 was investigated in synaptic structures in the rat superficial spinal dorsal horn laminae by using a pre-embedding immunogold technique. Immunostaining for mGluR2/3 occurred in laminae I through III. Gold particles were encountered both in the cytosol and along the plasma membrane. Distinctive plasmalemmal immunodeposits were detected in vesicle-containing profiles, where they were located to membrane compartments distant from active release sites rather than in the close vicinity of synaptic specialisations. No distinct immunolabelling was observed in profiles meeting characteristics of primary afferent terminals. The extrasynaptic occurrence of mGluR2/3 suggests a presynaptic heteroreceptor role for these receptor subtypes in the spinal dorsal horn.
authors.affiliation: Department of Neurosciences, School of Medicine and Dentistry, University of the Basque Country, Bilbao, Spain. onbazbaj@lg.ehu.es
authors.forename: J J
authors.initials: JJ
authors.lastname: Azkue
authors.forename: J M
authors.initials: JM
authors.lastname: Mateos
authors.forename: I
authors.initials: I
authors.lastname: Elezgarai
authors.forename: R
authors.initials: R
authors.lastname: Benítez
authors.forename: A
authors.initials: A
authors.lastname: Osorio
authors.forename: J
authors.initials: J
authors.lastname: Díez
authors.forename: A
authors.initials: A
authors.lastname: Bilbao
authors.forename: A
authors.initials: A
authors.lastname: Bidaurrazaga
authors.forename: P
authors.initials: P
authors.lastname: Grandes
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D020671:Posterior Horn Cells / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000032:analysis*; D013569:Synapses / Q000737:chemistry*
chemical_list: D018094:Receptors, Metabotropic Glutamate; C108822:metabotropic glutamate receptor 2; C102718:metabotropic glutamate receptor 3