Identification of a novel FGF, FGF-21, preferentially expressed in the liver.
We isolated cDNA encoding a novel FGF (210 amino acids) from mouse embryos. As this is the 21st documented FGF, we tentatively term it FGF-21. FGF-21 has a hydrophobic amino terminus ( approximately 30 amino acids), which is a typical signal sequence, and appears to be a secreted protein. The expression of FGF-21 mRNA in mouse adult tissues was examined by Northern blotting analysis. FGF-21 mRNA was most abundantly expressed in the liver, and also expressed in the thymus at lower levels. We also isolated human cDNA encoding FGF-21 (209 amino acids). Human FGF-21 is highly identical ( approximately 75% amino acid identity) to mouse FGF-21. Among human FGF family members, FGF-21 is most similar ( approximately 35% amino acid identity) to FGF-19.
Nishimura
T
T
Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Yoshida-Shimoadachi, Sakyo, Kyoto 606-8501,Japan.
Nakatake
Y
Y
Konishi
M
M
Itoh
N
N
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000032:analysis; D004622:Embryo, Mammalian / Q000502:physiology; D005346:Fibroblast Growth Factors / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors