Synthesis and fluorescence properties of new fluorescent, polymerizable, metal-chelating lipids.
Liposomes incorporating fluorescent, metal-chelating lipids find applications in molecular recognition of peptides, 2D protein recrystallization, protein targeting, and biological sensing. It would be advantageous to combine the usefulness of polymerizable, metal-chelating lipids and fluorescent lipids. Herein, we report the synthesis and fluorescence properties of several fluorescent, polymerizable, metal-chelating lipids. They have been successfully incorporated into liposomes and then polymerized. These lipids can be used as membrane probes to study the polymerizable liposomes in the unpolymerized state and to investigate lipid redistribution during polymerization. In addition, if a luminescent metal ion (e.g., Eu(3+), Tb(3+), etc.) is used to complex the headgroup, the lipids can probe the membrane interior and exterior simultaneously.
Roy
B C
BC
Department of Chemistry, North Dakota State University, Fargo, North Dakota 58105, USA.
Peterson
R
R
Mallik
S
S
Campiglia
A D
AD
D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D003374:Coumarins; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry*; D008670:Metals; D008854:Microscopy, Electron; D008968:Molecular Conformation; D009602:Nitrosamines; D012235:Rhodamines
D002614:Chelating Agents; D003374:Coumarins; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008670:Metals; D009602:Nitrosamines; D012235:Rhodamines; C013622:nitrosobis(2-oxopropyl)amine; C030123:coumarin; C029773:rhodamine B