title: A novel and single step ultrastructural method for staining biological tissues embedded in common embedding media.
abstract: A new single step staining method is described which is very simple, rapid and reproducible with better contrast than the widely employed uranyl acetate-lead citrate double staining method for observation of cell organelles in various tissue sections using transmission electron microscope. A copper uranyl acetate solution was employed to stain the ultrathin sections of tissues embedded in either water-miscible or water-immiscible resin media. The staining results are excellent. Cell organelles and extracellular matrix are stained with no artifactual precipitation or granulation.
authors.affiliation: Department of Biophysics and Electron Microscopy, Central Leather Research Institute, Adyar, Chennai, India.
authors.forename: B
authors.initials: B
authors.lastname: Lokanadam
authors.forename: P D
authors.initials: PD
authors.lastname: Gupta
authors.forename: T
authors.initials: T
authors.lastname: Ramasami
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000648:ultrastructure; D004396:Coloring Agents; D007422:Intestines / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D010870:Pineal Gland / Q000648:ultrastructure; D051381:Rats; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D004396:Coloring Agents