[The effect of methylated cyclodextrin on pertussis toxin accumulation in a Bordetella pertussis culture in a bioreactor].
The results obtained in the study of the influence methylated cyclodextrin (beta CD) on the growth of B. pertussis and the accumulation of pertussis toxin in the course of submerged batch cultivation in a bioreactor are presented. As demonstrated by these results, the presence of beta CD in the culture medium in a dose of 0.1 ml/l in the growth deceleration phase causes a tenfold increase in the synthesis of pertussis toxin by microbial cells in comparison with conditions characterized by the absence of beta CD. It cannot by ruled out that beta CD may act as a stressor which influences the synthesis of pertussis toxin, protector protein making it possible for the microbe to survive under new conditions.
Siundiukova
R A
RA
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
Basnak'ian
I A
IA
Stukalova
N V
NV
Aleksakhina
N N
NN
D019149:Bioreactors / Q000706:statistics & numerical data; D001886:Bordetella pertussis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D002648:Child; D016001:Confidence Intervals; D003470:Culture Media; D003505:Cyclodextrins / Q000494:pharmacology*; D006801:Humans; D008745:Methylation; D037342:Pertussis Toxin; D013268:Stimulation, Chemical; D010566:Virulence Factors, Bordetella / Q000032:analysis / Q000096:biosynthesis*; D014917:Whooping Cough / Q000382:microbiology
D003470:Culture Media; D003505:Cyclodextrins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin
Vliianie metilirovannogo tsiklodekstrina na nakoplenie kokliushnogo toksina v kul'ture Bordetella pertussis v bioreaktore.