title: Homogeneous longitudinal profiles and synchronous fluctuations of mitochondrial transmembrane potential.
abstract: This study reports for the first time (a) the longitudinal profile of the transmembrane potential (mDeltapsi) of single mitochondria using a Nernstian fluorescent probe and (b) the distribution of mDeltapsi fluctuations of mitochondria undergoing permanent depolarization. Our findings show that (1) mitochondria in different energetic conditions coexist in the same cell, (2) mDeltapsi is rather homogeneous along the entire length of single mitochondria, (3) mDeltapsi is not influenced by the surrounding cytoplasmic environment and (4) mDeltapsi fluctuations occur simultaneously in groups of mitochondria connected in a network. Taken together, these findings provide further evidence for a functional relationship between mitochondrial arrangement and energetic condition.
authors.affiliation: Department of Cytomorphology, University of Cagliari, Cittadella Universitaria, 09042, Monserrato, Italy. gdiaz@unica.it
authors.forename: G
authors.initials: G
authors.lastname: Diaz
authors.forename: A M
authors.initials: AM
authors.lastname: Falchi
authors.forename: F
authors.initials: F
authors.lastname: Gremo
authors.forename: R
authors.initials: R
authors.lastname: Isola
authors.forename: A
authors.initials: A
authors.lastname: Diana
mesh_terms: D001253:Astrocytes / Q000502:physiology / Q000648:ultrastructure; D002460:Cell Line; D006801:Humans; D007425:Intracellular Membranes / Q000502:physiology; D008564:Membrane Potentials; D008928:Mitochondria / Q000502:physiology*