title: Adenosine A1 receptors modulate high voltage-activated Ca2+ currents and motor pattern generation in the xenopus embryo.
abstract: Adenosine causes voltage- and non-voltage-dependent inhibition of high voltage-activated (HVA) Ca2+ currents in Xenopus laevis embryo spinal neurons. As this inhibition can be blocked by 8-cyclopentyl-1, 3-dipropylxanthine (DPCPX) and mimicked by N6-cyclopentyladenosine (CPA) it appears to be mediated by A1 receptors. Agents active at A2 receptors either were without effect or could be blocked by DPCPX. AMP had no agonist action on these receptors. By using omega-conotoxin GVIA we found that adenosine inhibited an N-type Ca2+ current as well as a further unidentified HVA current that was insensitive to dihydropyridines, omega-agatoxin TK and omega-conotoxin MVIIC. Both types of current were subject to voltage- and non-voltage-dependent inhibition. We used CPA and DPCPX to test whether A1 receptors regulated spinal motor pattern generation in spinalized Xenopus embryos. DPCPX caused a near doubling of, while CPA greatly shortened, the length of swimming episodes. In addition, DPCPX slowed, while CPA greatly speeded up, the rate of run-down of motor activity. Our results demonstrate a novel action of A1 receptors in modulating spinal motor activity. Furthermore they confirm that adenosine is produced continually throughout swimming episodes and acts to cause the eventual termination of activity.
authors.affiliation: School of Biological Sciences, Bute Medical Building, University of St Andrews, St Andrews, Fife KY16 9TS, UK.
authors.forename: P
authors.initials: P
authors.lastname: Brown
authors.forename: N
authors.initials: N
authors.lastname: Dale
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020864:Calcium Channels, N-Type / Q000502:physiology; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D004625:Embryo, Nonmammalian / Q000166:cytology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005415:Flavins / Q000494:pharmacology; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009046:Motor Neurons / Q000737:chemistry / Q000502:physiology*; D018408:Patch-Clamp Techniques; D018047:Receptors, Purinergic P1 / Q000502:physiology*; D013126:Spinal Nerve Roots / Q000166:cytology; D013550:Swimming / Q000502:physiology; D014970:Xanthines / Q000494:pharmacology; D014981:Xenopus; D020866:omega-Conotoxin GVIA / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D020864:Calcium Channels, N-Type; D015226:Dinucleoside Phosphates; D004791:Enzyme Inhibitors; D005415:Flavins; D018047:Receptors, Purinergic P1; D014970:Xanthines; C013907:cytidylyl adenosine; D000249:Adenosine Monophosphate; C008173:isoalloxazine; D020866:omega-Conotoxin GVIA; C051360:1,3-dipropyl-8-cyclopentylxanthine; D000241:Adenosine; D002118:Calcium