[Effect of the protein kinase C inhibitor on changes in Ca(2+) level in pig granulosa cells induced by prolactin].
The effect of the PKC inhibitor on Ca2+ responses to prolactin in the pig granulosa cells was studied using fluorescent dye and chlortetracycline. The effect was shown to be connected with activation of the PKC. The Ro 31-8220 increased penetration of extracellular calcium and exit of calcium from intracellular stores. The data obtained suggest an involvement of the PKC in changes of calcium contents in the pig granulosa cells activated by prolactin.
Denisenko
V Iu
VIu
Research Institute of Farm Animal Genetics and Breeding, St. Petersburg-Pushkin, Russia.
Kuz'mina
T I
TI
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology*; D011388:Prolactin / Q000494:pharmacology / Q000502:physiology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013552:Swine
D004791:Enzyme Inhibitors; D007211:Indoles; D011388:Prolactin; D011493:Protein Kinase C; C064758:Ro 31-8220
Vliianie ingibitora proteinkinazy C na vyzvannye prolaktinom izmenenia urovnia Ca(2+) v kletkakh granulozy svin'i.