Effect of aqueous extract of cigarette smoke on peripheral blood polymorphonuclear leukocytes chemiluminescence.
Cigarette smoke induces a vast cohort of deleterious effects on biological structures. In the present paper, the effect of aqueous extract of cigarette smoke on the activity of polymorphonuclear leukocytes was studied. Although the aqueous extract of cigarette smoke inhibits the luminol oxidation catalysed by horseradish peroxidase, it strongly interacts with polymorphonuclear leukocytes and inhibits their phorbol-induced chemiluminescence in the presence of either luminol or lucigenin. The results indicate that at least some of the components of the aqueous extract of cigarette smoke may strongly interfere with polymorphonuclear cells, contributing to the deleterious effects of smoke products.
Zappacosta
B
B
Istituto di Chimica e Chimica Clinica and CNR Centro Chimica dei Recettori Università Cattolica del Sacro Cuore, Rome, Italy. b.zappacosta@uniserv.ccr.rm.cnr.it
Persichilli
S
S
Giardina
B
B
De Sole
P
P
D000166:Acridines; D006735:Horseradish Peroxidase; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D010947:Plants, Toxic; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects* / Q000737:chemistry; D014867:Water
D000166:Acridines; D012906:Smoke; D014867:Water; C033472:10,10'-dimethyl-9,9'-biacridinium; D008165:Luminol; D006735:Horseradish Peroxidase