H-point curve isolation method for coupled liquid chromatography and UV-visible spectrophotometry.
The H-point curve isolation method (HPCIM) for the detection of unknown interferences in chromatography is proposed. The method allows one to estimate the UV-vis spectra of interfering species in a sample as well as to test the purity of the chromatographic peaks. Besides the detection of the unknown interferences in a sample, this method allows one to calculate the concentration of an analyte in the presence of unknown compounds. To illustrate the reliability of the proposed method, samples of diuretics and amphetamines have been analyzed by normal- and reversed-phase high-performance chromatography.
Blasco-Gómez
F
F
Departament de Química Analítica, Facultad de Química, Universidad de Valencia, Spain.
Bosch-Reig
F
F
Campíns-Falcó
P
P
Molins-Legua
C
C
Herráez-Hernández
R
R
D000662:Amphetamines / Q000032:analysis*; D002853:Chromatography, Liquid; D004232:Diuretics / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000662:Amphetamines; D004232:Diuretics