title: The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity.
abstract: The Bacillus pumilus gene encoding acetyl xylan esterase (axe) was identified and characterized. The axe gene was expressed and the recombinant enzyme produced in Escherichia coli was purified and characterized. The recombinant enzyme displayed similar properties to the acetyl xylan esterase (AXE) purified from B. pumilus. The AXE primary structure was 76% identical to the cephalosporin C deacetylase of B. subtilis, and 40% to two recently identified AXEs from Thermoanaerobacterium and Thermotoga maritima. These four proteins are of similar size and represent a new family of esterases having a broad substrate specificity. The recombinant AXE was demonstrated to have activity on several acetylated substrates, including on cephalosporin C.
authors.affiliation: Bacteriology Group, International Centre for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, I-34012 Trieste, Italy1.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Degrassi
authors.affiliation: Bacteriology Group, International Centre for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, I-34012 Trieste, Italy1.
authors.forename: Milan
authors.initials: M
authors.lastname: Kojic
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11000 Belgrade, Yugoslavia2.
authors.forename: Goran
authors.initials: G
authors.lastname: Ljubijankic
authors.affiliation: Bacteriology Group, International Centre for Genetic Engineering and Biotechnology, Area Science Park, Padriciano 99, I-34012 Trieste, Italy1.
authors.forename: Vittorio
authors.initials: V
authors.lastname: Venturi
mesh_terms: D000115:Acetylesterase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001421:Bacteria, Anaerobic / Q000201:enzymology / Q000235:genetics; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases; C019861:cephalosporin-C deacetylase; D000115:Acetylesterase; C081851:acetylxylan esterase