title: Interaction of plant polysomes with the actin cytoskeleton.
abstract: Protein composition and functional activity of various polysome subpopulations isolated from Vicia faba L. leaves and Triticum aestivum L. and Hordeum vulgare L. seedlings were studied. Membrane- and cytoskeleton-bound polysomes were more active in the wheat germ cell-free translational system than free polysomes. Several non-ribosomal proteins were detected in the polysome preparations by gel electrophoresis and Western blot analysis: (1) a canonical actin of mol wt 42 kDa; (2) a 40 kDa protein, demonstrating affinity for ribosomes, sharing some determinants with actin, and present predominantly in the subpopulations of bound polysomes; and (3) an acidic ribosome-associated p40 evenly distributed between free and bound polysomes. The possibility of involvement of these proteins in interactions between polysomes and the actin cytoskeleton is discussed.
authors.affiliation: Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya ul. 35, Moscow. ifr@ippras.ru
authors.forename: N
authors.initials: N
authors.lastname: Klyachko
authors.forename: L
authors.initials: L
authors.lastname: Aksenova
authors.forename: M
authors.initials: M
authors.lastname: Dunaeva
authors.forename: A
authors.initials: A
authors.lastname: Kulikova
mesh_terms: D000199:Actins / Q000378:metabolism*; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003599:Cytoskeleton / Q000201:enzymology / Q000378:metabolism*; D007887:Fabaceae; D001467:Hordeum; D006868:Hydrolysis; D008970:Molecular Weight; D018515:Plant Leaves / Q000201:enzymology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000201:enzymology / Q000378:metabolism; D010946:Plants, Medicinal; D011132:Polyribosomes / Q000201:enzymology / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism; D014908:Triticum
chemical_list: D000199:Actins; D010940:Plant Proteins; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase