Capillary electrophoresis with postcolumn infectivity assay for the analysis of different serotypes of human rhinovirus (common cold virus).
Differentiation of virus serotypes with capillary zone electrophoresis was demonstrated. For four serotypes of human rhinovirus (HRV2, HRV14, HRV16, HRV49), different electrophoretic mobility was achieved at pH 8.3 (borate/boric acid buffer, 100 mmol/L). Addition of detergent (Triton X-100-R, deoxycholate, and/or SDS) to the background electrolyte was required for reduction of wall adsorption and improvement of peak shape. A major nonviral contaminant, present in all virus samples, was best separated from the viral peaks with 10 mmol/L SDS as additive. The method allowed detecting serotypes HRV16 and HRV49 in crude, partially purified virus preparations. An infectivity assay carried out off-line with fractions collected at the capillary outlet enabled the sensitive and biospecific identification of the peaks of HRV2 and HRV14.
Okun
V
V
Institute of Analytical Chemistry, University of Vienna, Austria.
Ronacher
B
B
Blaas
D
D
Kenndler
E
E
D019075:Electrophoresis, Capillary; D006367:HeLa Cells; D006801:Humans; D012229:Rhinovirus / Q000737:chemistry* / Q000145:classification / Q000472:pathogenicity*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013056:Spectrophotometry, Ultraviolet