Comparison of DNA extraction from blood stain and decomposed muscle in STR polymorphism analysis.
Forensic samples that are often degraded and limited in quality cause DNA typing analysis by conventional methods unsuitable. We performed a single tube-multiplex PCR on 9 STR loci (D3S1358, vWA, FGA, TH01, TPOX, CSF1PO, D5S818, D13S317, and D7S820) and the X-Y homologous gene amelogenin of DNA extracted from six week postmortem blood stain and decomposed muscle by using QIAGEN QIAamp blood or tissue procedure. An automated genetic analyzer based on fluorescent dye technology was used to detect STR allele patterns. The DNA profile of blood stain sample obtained a complete and unambiguous pattern, whereas, that of muscle DNA extracted from QIAamp tissue and Chelex plus QIAamp blood protocols showed detected STR alleles for 70 per cent and 50 per cent of all tested alleles, respectively. The degraded muscle DNA could not yield amplified products of large size STR alleles; CSF1PO, D13S317 and D7S820. However, the analysis which relied upon the PCR-based STR polymorphism analysis and automated genetic analyzer system offers an ideal strategy for forensic identification.
Rerkamnuaychoke
B
B
Human Genetics Unit, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Chantratita
W
W
Jomsawat
U
U
Thanakitgosate
J
J
Pattanasak
N
N
Rojanasunan
P
P
D001344:Autopsy; D001801:Blood Stains; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting; D006801:Humans; D018482:Muscle, Skeletal / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012680:Sensitivity and Specificity; D020080:Tandem Repeat Sequences
D004247:DNA