A new Klebsiella planticola strain (Cd-1) grows anaerobically at high cadmium concentrations and precipitates cadmium sulfide.
Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl(2) under a wide range of NaCl concentrations and at acidic or neutral pH. In growth medium amended with thiosulfate, it precipitated significant amounts of cadmium sulfide (CdS), as confirmed by x-absorption spectroscopy. In comparison with various other strains tested, Cd-1 is superior for precipitating CdS in cultures containing thiosulfate. Thus, our results suggest that Cd-1 is a good candidate for the accelerated bioremediation of systems contaminated by high levels of cadmium.
Sharma
P K
PK
Department of Applied Science, Brookhaven National Laboratory, Upton, New York 11973, USA.
Balkwill
D L
DL
Frenkel
A
A
Vairavamurthy
M A
MA
D000693:Anaerobiosis; D002104:Cadmium / Q000378:metabolism* / Q000494:pharmacology; D019187:Cadmium Compounds / Q000378:metabolism*; D011232:Chemical Precipitation; D015169:Colony Count, Microbial; D003470:Culture Media; D005798:Genes, Bacterial; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D007709:Klebsiella / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D013440:Sulfides / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism
D019187:Cadmium Compounds; D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D013440:Sulfides; D013885:Thiosulfates; D002104:Cadmium; C034939:cadmium sulfide; D012965:Sodium Chloride