Molecular cloning of a gene encoding a 20S proteasome beta subunit from Plasmodium falciparum.
A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome beta-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a 156-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding.
Li
G D
GD
Department of Pharmacology and Therapeutics, The University of Liverpool, L69 3BX, Liverpool, UK.
Li
J L
JL
Mugthin
M
M
Ward
S A
SA
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D010963:Plasmodium falciparum / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D051381:Rats; D016415:Sequence Alignment; D014982:Xenopus laevis; D015027:Zebrafish
D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases