title: Synechocystis strain PCC 6803 cya2, a prokaryotic gene that encodes a guanylyl cyclase.
abstract: Synechocystis strain PCC 6803 exhibits similar levels of cyclic AMP (cAMP) and cyclic GMP (cGMP). A thorough analysis of its genome showed that Cya2 (Sll0646) has all the sequence determinants required in terms of activity and purine specificity for being a guanylyl cyclase. Insertional mutagenesis of cya2 caused a marked reduction in cGMP content without altering the cAMP content. Thus, Cya2 represents the first example of a prokaryotic guanylyl cyclase.
authors.affiliation: Dynamique des Membranes Végétales, Complexes Protéines-Pigments, CNRS UMR8543, Ecole Normale Supérieure, 75230 Paris Cedex 05, France. jhoumard@biologie.ens.fr
authors.forename: J A
authors.initials: JA
authors.lastname: Ochoa De Alda
authors.forename: G
authors.initials: G
authors.lastname: Ajlani
authors.forename: J
authors.initials: J
authors.lastname: Houmard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D006162:Guanylate Cyclase / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D000242:Cyclic AMP; D006162:Guanylate Cyclase; D006152:Cyclic GMP