Characterization of gammadelta T cells expressing CD158b, a killer cell inhibitory receptor, in a patient with chronic CD4(+) lymphocytopenia and disseminated Mycobacterium intracellulare infection.
A population of Vdelta1(+)Vgamma9(-) gammadelta T cells that represented almost the totality (84%) of circulating lymphocytes in a patient with chronic, non-HIV-related, CD4 lymphocytopenia complicated by a disseminated Mycobacterium intracellulare infection was characterized. These gammadelta(+) T cells expressed a single killer inhibitory receptor (CD158b) and their phenotype (CD8(+)CD57(+)CD27(-)CD28(-)) indicated that, although CD45RA(+), they were not naive. However, the absence of large granular lymphocyte morphology, the impaired proliferative activity, the high susceptibility to apoptosis, and the total lack of cytotoxic ability suggested that these gammadelta cells were in a resting state. A high percentage of the cells did not harbor the CD11b integrin alpha chain and exhibited a decreased capability to bind endothelial cells. This defect might represent the mechanism whereby they remained trapped in the circulation.
Airò
P
P
Servizio di Immunologia Clinica, Spedali Civili, Brescia, Italy.
Caruso
A
A
Stellini
R
R
Antonioli
C
C
Malacarne
F
F
Licenziati
S
S
Albertini
A
A
Cattaneo
R
R
Imberti
L
L
D015703:Antigens, CD / Q000145:classification; D017209:Apoptosis / Q000276:immunology; D018106:CD28 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002448:Cell Adhesion; D002455:Cell Division; D002908:Chronic Disease; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000276:immunology; D008875:Middle Aged; D015269:Mycobacterium avium Complex / Q000276:immunology; D015270:Mycobacterium avium-intracellulare Infection / Q000097:blood / Q000150:complications / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000276:immunology; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3; D018344:T-Lymphocytopenia, Idiopathic CD4-Positive / Q000097:blood / Q000150:complications / Q000276:immunology*
D015703:Antigens, CD; D018106:CD28 Antigens; D016827:CD8 Antigens; D016177:Macrophage-1 Antigen; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3