title: Molecular identification and characterization of novel members of the human organic anion transporter (OATP) family.
abstract: We identified three novel transporters structurally belonging to the organic anion transporting polypeptide (OATP) family in humans. Since previously known rat oatp1 to 3 do not necessarily correspond to the human OATPs in terms of either tissue distribution or function, here we designate the newly identified human OATPs as OATP-B, -D and -E, and we rename the previously known human OATP as OATP-A. OATP-C proved to be identical with the recently reported LST1/OATP-2. Expression profiles of the five OATPs and the prostaglandin transporter PGT (a member of OATP family) in human tissues showed that OATP-C is exclusively localized in liver, OATP-A and PGT are expressed in restricted ranges of tissues, and OATP-B, -D and -E show broad expression profiles. OATP-B, -C, -D and -E exhibited transport activity for [(3)H]estrone-3-sulfate as a common substrate. OATP-C has a high transport activity with broad substrate specificity.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, 920-0934, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Tamai
authors.forename: J
authors.initials: J
authors.lastname: Nezu
authors.forename: H
authors.initials: H
authors.lastname: Uchino
authors.forename: Y
authors.initials: Y
authors.lastname: Sai
authors.forename: A
authors.initials: A
authors.lastname: Oku
authors.forename: M
authors.initials: M
authors.lastname: Shimane
authors.forename: A
authors.initials: A
authors.lastname: Tsuji
mesh_terms: D000595:Amino Acid Sequence; D027321:Anion Transport Proteins; D017920:Antiporters / Q000737:chemistry / Q000235:genetics; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D015232:Dinoprostone / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism; D004970:Estrone / Q000031:analogs & derivatives / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009928:Organ Specificity; D027361:Organic Anion Transporters; D010400:Penicillin G / Q000378:metabolism; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D013379:Substrate Specificity; D014162:Transfection
chemical_list: D027321:Anion Transport Proteins; D017920:Antiporters; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D027361:Organic Anion Transporters; D012333:RNA, Messenger; C503526:SLCO2A1 protein, human; C503528:Slco2a1 protein, rat; C025483:estradiol-17 beta-glucuronide; D004970:Estrone; D004958:Estradiol; D015232:Dinoprostone; D010400:Penicillin G; C017296:estrone sulfate