Enantiomorph determination using inverse reference-beam diffraction images.
It is shown that enantiomorph structures of a noncentrosymmetric crystal can be determined, in the absence of anomalous diffraction signals, by measuring two series of reference-beam oscillation diffraction patterns related by an inverse-beam geometry. The corresponding intensities of the Friedel pairs recorded on the two sets of images exhibit the characteristic three-beam interference effects that provide the unambiguous phase information. The experimental arrangement and the data-analysis procedure are demonstrated through an experimental example on tetragonal lysozyme.
Shen
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Q
Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, NY 14853, USA. qs11@cornell.edu
Kycia
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S
Dobrianov
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I
D018360:Crystallography, X-Ray / Q000379:methods*; D009113:Muramidase / Q000737:chemistry
D009113:Muramidase