Peroxidase activity in the submandibular gland of the house musk shrew, Suncus murinus (Soricidae, Insectivora).
Endogenous peroxidase activity in the submandibular gland of the house musk shrew, Suncus murinus was cytochemically investigated by light and electron microscopy using 3,3'-diaminobenzidine-tetrahydrochloride salt (DAB). The submandibular glands of male Suncus murinus at 8-month-olds were excised and diced into small pieces. In general, salivary glands are structurally divided into a terminal portion comprising a secretory portion and duct system. The submandibular gland of the Suncus murinus, the terminal portions consisted of proximal and distal acinar cells. On the other hand, a granular duct cell of the duct system contained a number of characteristic myelin-like bodies. In the present study, the peroxidase reaction products were localized in the secretory granules of the proximal acinar cells and in the endoplasmic reticulum, Golgi apparatus and myelin-like bodies of the granular duct cells. These reaction products were reduced when 5 mM 3-amino-1,2,4-triazole was added to the reaction medium. Additionally, release of peroxidase into the lumen was observed. In conclusion, the proximal acinar and granular duct cells formed peroxidase and may have performed excretory secretions. Moreover, the peroxidase positive myelin-like body consisted of lamellated membrane and its outer surface membrane continued to the endoplasmic reticulum.
Moriguchi
K
K
Department of 1st Anatomy, School of Dentistry, Aichi-Gakuin University, Japan.
Utsumi
M
M
Hanamura
H
H
Ohno
N
N
D000818:Animals; D008297:Male; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism*; D012788:Shrews / Q000502:physiology*; D013363:Submandibular Gland / Q000201:enzymology* / Q000648:ultrastructure
D009195:Peroxidase