Effect of dentin primers on the morphology, molecular composition and collagen conformation of acid-demineralized dentin in situ.
OBJECTIVE
The purpose of this study was to assess the effect of two commercial primers and distilled water on the morphology, molecular composition and collagen conformation of acid-demineralized dentin in situ.


METHODS
Dentin specimens etched with Scotchbond Etchant were imaged by tapping mode AFM and analyzed by MIR-FTIR spectroscopy. They were then primed with Scotchbond Multi-Purpose Plus Primer, Scotchbond 1 Adhesive or distilled water and imaged and analyzed again. The chemical modifications induced on the uppermost 2 microns of primed dentin were studied after water and original primer subtraction. The conformational changes of type I collagen at this region were evaluated by deconvoluting the amide I band components. The absorbance ratio An(1655/1627) was used to semiquantitatively assess, on a relative basis, the extent of collagen denaturation.


RESULTS
All the priming treatments swelled the collapsed dentin collagen left after etching. No evidence of primary bonding was found after priming treatments, while approximately 50% of the conditioned dentin collagen appeared denatured. Treatment with distilled water did not change the status of denatured collagen, however, application of the commercial primers refolded the alpha-helix to approximately 95% of the extent found on the native reference dentin.


CONCLUSIONS
The dynamic response of dentin collagen to demineralization and priming treatments reveals the critical role of some primers in rapidly restoring the conformational status of acid-denatured collagen. Implementation of reactive adhesive groups in alpha-helix recovery may provide an associative means of modifying the mechanical properties of the demineralized collagen based on the extent of their intermolecular bonding.
Eliades
G
G
Research Center for Biomaterials, Athens, Greece. ekevyl@hol.gr
Vougiouklakis
G
G
Palaghias
G
G
D000134:Acid Etching, Dental; D000704:Analysis of Variance; D003094:Collagen / Q000187:drug effects; D003804:Dentin / Q000737:chemistry / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D019279:Resin Cements; D017550:Spectroscopy, Fourier Transform Infrared; D018709:Statistics, Nonparametric
D017220:Dentin-Bonding Agents; D019279:Resin Cements; C084018:Scotchbond Multi-Purpose; D003094:Collagen