Tumor cell growth suppression by tannic acid.
Tannic acid was cultured together with tumor cells that had originated from human malignant tumors (HCT-15 & AGS). Significant suppression of tumor growth was observed. The 50% suppression was observed at the concentration between 50 micrograms/ml and 12.5 micrograms/ml. When tannic acid was injected into HCT-15 cells by electroporation at 1180 mu p and 200 ohm, the suppression rate was 34.3% at the concentration of 50 micrograms/ml in HBS solution. The suppression rate of cells only in contact with tannic acid solution for one hour under the same conditions as used for electroporation, was 26.1%. Histographic findings suggested that tannic acid almost completely blocked the S phase of the cell cycle.
Kamei
H
H
Department of Surgery, Aichi-Gakuin University Hospital, Nagoya, Japan. kamei@dpc.aichi-gakuin.ac.jp
Koide
T
T
Hashimoto
Y
Y
Kojima
T
T
Hasegawa
M
M
D000230:Adenocarcinoma / Q000473:pathology*; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000473:pathology*; D004261:DNA Replication / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis; D004354:Drug Screening Assays, Antitumor; D018274:Electroporation; D006801:Humans; D047348:Hydrolyzable Tannins / Q000008:administration & dosage / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D047348:Hydrolyzable Tannins; D019384:Nucleic Acid Synthesis Inhibitors