title: Oxygen radical generation of neutrophils: a reason for oxidative stress during marathon running?
abstract: Hematological parameters and blood markers that indicate oxidative stress, such as lipid peroxides (LPO), reduced and oxidized glutathione (GSH, GSSG), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), were measured in 18 marathon runners before, immediately after the race, and after 8 days of rest. In parallel, the oxygen radical generation of neutrophils (PMN) was measured by chemiluminescence in six randomly selected runners. After the race, a 4.4-fold enhanced PMN count and a 1.4-fold increased capacity to generate oxygen radicals of the PMN (2.20+/-0.38 vs. 3.12+/-0.69 arb. unit/10(6) cells) were found. Consequently, a 6.25-fold increased capacity to generate oxygen radicals of the post-run blood (7.26+/-1.3 vs. 45.40+/-10.3 arb. unit/ml blood) was calculated. This points to PMN as an important oxygen radical source established in the runners' blood, which could contribute to the oxidative stress indicated in the post-run blood by increased LPO (11.46+/-3.09 vs. 13.09+/-3.14 micromol/l plasma), GSSG (0.038+/-0.003 vs. 0.045+/-0. 005 mmol/l blood) and GSSG/GSH ratio (3.8+/-0.5 vs. 4.1+/-0.6%) and by decreased SOD (15.63+/-1.78 vs. 14.58+/-1.51 10(3)U/mmol Hb) and GSH-Px (485.1+/-107.1 vs. 434.9+/-101.7 U/mmol Hb). Despite the decline of the oxygen radical source during rest, the oxidative stress in the blood did not decrease in all runners.
authors.affiliation: Institut für Medizinische Physik und Biophysik, Humboldt-Universität, D-10098, Berlin, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Hessel
authors.forename: A
authors.initials: A
authors.lastname: Haberland
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: D
authors.initials: D
authors.lastname: Lerche
authors.forename: I
authors.initials: I
authors.lastname: Schimke
mesh_terms: D000328:Adult; D005609:Free Radicals; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007958:Leukocyte Count; D008054:Lipid Peroxides / Q000097:blood*; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009211:Myoglobin / Q000097:blood; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D012420:Running / Q000502:physiology*; D013482:Superoxide Dismutase / Q000097:blood; D013481:Superoxides
chemical_list: D005609:Free Radicals; D008054:Lipid Peroxides; D009211:Myoglobin; D017382:Reactive Oxygen Species; D013481:Superoxides; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione