title: Massive accumulation of M and H subunits of neurofilament proteins in spinal motor neurons of neurofilament deficient Japanese quail, Quv.
abstract: Quiver (Quv) is a non-sense mutation of neurofilament protein L subunit (NF-L) that causes neurofilament deficiency with preserved microtubules in Japanese quail. Anti-NF-M and anti-NF-H mAbs stained cell bodies of motor neurons in Quv embryo spinal cords much more intense than those in control spinal cords. Volume of motor neurons in Quv spinal cords increased to 2.3 times of control motor neurons. Immunoblot of Quv spinal cords revealed a relative increase in non- and hypo-phosphorylated NF-M and NF-H, and a decrease in the total amount of NFs. Quv sciatic nerves showed faintly reacted phosphorylated NF-M and NF-H. These results suggest that deficiency of assembled neurofilament results in decreased axonal transport of NFs and accumulation of NFs in cell bodies of spinal motor neurons.
authors.affiliation: Department of Internal Medicine, Akita University School of Medicine, Japan. toyoshim@med.akita-u.ac.jp
authors.forename: I
authors.initials: I
authors.lastname: Toyoshima
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Sugawara
authors.forename: C
authors.initials: C
authors.lastname: Wada
authors.forename: S
authors.initials: S
authors.lastname: Okawa
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: O
authors.initials: O
authors.lastname: Masamune
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001370:Axonal Transport / Q000235:genetics; D002642:Chick Embryo; D003370:Coturnix / Q000235:genetics*; D004195:Disease Models, Animal; D015151:Immunoblotting; D016472:Motor Neuron Disease / Q000235:genetics / Q000378:metabolism; D009046:Motor Neurons / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D000911:Antibodies, Monoclonal; D016900:Neurofilament Proteins; C044882:neurofilament protein L; C061195:neurofilament protein M