[The development and testing of test systems for the determination of herpes simplex virus and cytomegalovirus antigens by lanthanide immunofluorescence analysis].
On the basis of the lanthanide immunofluorescent assay (LIFA) test systems for the determination of herpes simplex virus (HSV-LIFA) and cytomegalovirus (CMV-LIFA) antigens have been developed. The test system HSV-LIFA includes the sandwich of monoclonal antibodies (McAb) HSV A.3.3. or B-11 to type 2 HSV, strain BH; the test system CMV-LIFA includes the sandwich of rabbit McAb to CMV, strain AD 169. The approbation of the test systems has revealed that they insure the specific detection of HSV and CMV antigens in clinical specimens (urine, blood, liquor, saliva), the LIFA results well correlating with the data on the isolation of viruses in cell cultures, with the results obtained by other diagnostic methods and with clinical manifestations of diseases. LIFA has been shown to be more sensitive than the enzyme immunoassay.
Pomelova
V G
VG
State Research Institute of Biological Instruments, Moscow, Russia.
Bychenkova
T A
TA
Grigor'eva
A M
AM
Masalova
O V
OV
Zubkov
V V
VV
Barinskiĭ
I F
IF
L'vov
N D
ND
Kushch
A A
AA
Ivanova
L A
LA
Kudashov
N I
NI
Osin
N S
NS
Khramov
E N
EN
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001826:Body Fluids / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008674:Metals, Rare Earth; D051379:Mice; D011817:Rabbits; D011933:Reagent Kits, Diagnostic / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D000956:Antigens, Viral; D008674:Metals, Rare Earth; D011933:Reagent Kits, Diagnostic
Razrabotka i aprobatsiia test-sistem dlia opredeleniia antigenov virusa prostogo gerpesa i tsitomegalovirusa metodom lantanidnogo immunofliuorestsentnogo analiza.