title: Comparison of polymorphism of thymidine kinase gene and restriction fragment length polymorphism of genomic DNA in herpes simplex virus type 1.
abstract: The polymorphism of the thymidine kinase (TK) gene of herpes simplex virus type 1 (HSV-1) was analyzed and was compared with the restriction fragment length polymorphism (RFLP) of the whole genome to evaluate the relative efficiency of the TK gene as a potential probe for identification and discrimination of HSV-1. The effectiveness of using the polymorphism of the TK gene in classifying HSV-1 strains was comparable to that of RFLP analysis of 66 sites, suggesting that TK gene sequencing may have important applications in epidemiological studies of HSV-1.
authors.affiliation: Department of Otolaryngology, Asahikawa Medical College, Asahikawa, 078-8510, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagamine
authors.forename: T
authors.initials: T
authors.lastname: Suzutani
authors.forename: M
authors.initials: M
authors.lastname: Saijo
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: M
authors.initials: M
authors.lastname: Azuma
mesh_terms: D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D016679:Genome, Viral; D006561:Herpes Simplex / Q000453:epidemiology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D013937:Thymidine Kinase / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D013937:Thymidine Kinase