Synthesis of glycosylated polyethylenimine with reduced toxicity and high transfecting efficiency.
A safe and efficient synthesis of glycosylated polyethylenimine using titanium (IV) isopropoxide and sodium borohydride has been carried out as a substitute for the highly toxic sodium cyanoborohydride method currently used. Poryplexes formed between DNA and the various glycosylated polyethylenimines appeared to be much less cytotoxic than polyethylenimine (PEI)/DNA polyplexes.
Leclercq
F
F
Laboratoire de Chimie Bioorganique et Biotechnologie Moleculaire et cellulaire, Unité Mixte ENSCP/CNRS/AVENTIS (UMR 7001), ENSCP, Paris, France. fralecle@ext.jussieu.fr
Dubertret
C
C
Pitard
B
B
Scherman
D
D
Herscovici
J
J
D002470:Cell Survival / Q000187:drug effects; D006031:Glycosylation; D011094:Polyethyleneimine / Q000138:chemical synthesis* / Q000494:pharmacology* / Q000633:toxicity; D014162:Transfection; D014407:Tumor Cells, Cultured
D011094:Polyethyleneimine