Detection of intracellular bacteria in the buds of Scotch pine (Pinus sylvestris L.) by in situ hybridization.
Bacterial isolates were obtained from pine (Pinus sylvestris L.) tissue cultures and identified as Methylobacterium extorquens and Pseudomonas synxantha. The existence of bacteria in pine buds was investigated by 16S rRNA in situ hybridization. Bacteria inhabited the buds of every tree examined, primarily colonizing the cells of scale primordia and resin ducts.
Pirttilä
A M
AM
Department of Biology/Botany, University of Oulu, Oulu, Finland. AM>Pirttila@oulu.fi
Laukkanen
H
H
Pospiech
H
H
Myllylä
R
R
Hohtola
A
A
D032462:Cycadopsida / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D017403:In Situ Hybridization; D020601:Methylobacterium extorquens / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D041605:Pinus sylvestris; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S