Application of cross-species color banding (RxFISH) in the study of T-prolymphocytic leukemia.
OBJECTIVE
Cross-species color banding (RxFISH) is a new FISH technology based on the use of differentially labeled gibbon chromosome probes to obtain a specific color banding pattern for each human chromosome. The aim of the study was to test the RxFISH technique for better characterization of complex karyotypes in patients with T-prolymphocytic leukemia (T-PLL).


METHODS
The study evaluated the cross-species color banding technique in four patients affected with T-PLL previously studied by conventional cytogenetics.


RESULTS
All patients showed an abnormal karyotype and three of them had a complex karyotype. The involvement of 14q11 in all four cases, the gain of 8q in three cases and a loss of chromosome 10, 15 and 17 and a gain of chromosome 21 in two cases were noted. The RxFISH technique identified from 2 to 7 not previously recognized aberrations per case and confirmed the inv(14)(q11q32).


CONCLUSIONS
To our knowledge, this is the first application of RxFISH to characterize chromosomal rearrangements in T-cell neoplasms. RxFISH gave rapid and easy identification of chromosome rearrangements that were difficult to recognize by conventional cytogenetics. Using this new technology we identified 15 rearrangements not detected by conventional cytogenetics.
Espinet
B
B
Laboratori de Citologia Hematològica, Laboratori de Referència de Catalunya, Unitat d'Hematologia 1973, Barcelona, Spain. e0037@imas.imim.es.
Solé
F
F
Salido
M
M
Lloveras
E
E
Abella
E
E
Besses
C
C
Serrano
S
S
Woessner
S
S
Florensa
L
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002871:Chromosome Banding / Q000379:methods*; D007446:Chromosome Inversion; D002883:Chromosomes, Human, Pair 14; D003582:Cytogenetics; D015342:DNA Probes; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000592:standards; D007621:Karyotyping; D015463:Leukemia, Prolymphocytic / Q000235:genetics*; D015458:Leukemia, T-Cell / Q000235:genetics*; D008297:Male; D008722:Methods
D015342:DNA Probes