title: Participation of endogenous endothelins in delayed eosinophil and neutrophil recruitment in mouse pleurisy.
abstract: OBJECTIVE
  Investigate the role of endothelins in leukocyte recruitment in allergic and non allergic inflammation.
  
  
  METHODS
  Pleurisy was induced in mice by intrathoracic injection of ovalbumin (OVA; in sensitized animals), E. coli LPS, carrageenan, Mycobacterium bovis (BCG) or zymosan. Animals were treated with BQ-123 or BQ-788 (1.5-150 pmol/cavity), or intravenously with bosentan (30 mg/kg).
  
  
  RESULTS
  None of the ET receptor antagonists modified early neutrophil recruitment (at 4 h) induced by OVA, LPS, carrageenan, BCG or zymosan or plasma leakage caused by carrageenan or zymosan. Mononuclear and eosinophil accumulation triggered by OVA were reduced by BQ-123 (150 pmol/cavity) or bosentan (68 and 43% inhibition of eosinophilia), but unaffected by BQ-788. BQ-123 and bosentan also inhibited LPS increases in neutrophil (by 67 and 40%) and eosinophil (by 63 and 74%) at 24 h.
  
  
  CONCLUSIONS
  Endothelins, acting via ETA receptors, play a role in late eosinophil and neutrophil accumulation (24 h), but not in the acute (4 h) neutrophilic response.
authors.affiliation: Laboratório de Farmacologia Aplicada, Instituto de Tecnologia em Fármacos - FARMANGUINHOS - Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
authors.forename: A L
authors.initials: AL
authors.lastname: Sampaio
authors.forename: G A
authors.initials: GA
authors.lastname: Rae
authors.forename: M G
authors.initials: MG
authors.lastname: Henriques
mesh_terms: D000818:Animals; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000502:physiology*; D004804:Eosinophils / Q000502:physiology*; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000502:physiology*; D009842:Oligopeptides / Q000494:pharmacology; D010047:Ovalbumin; D010456:Peptides, Cyclic / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D010998:Pleurisy / Q000139:chemically induced / Q000473:pathology*; D011506:Proteins / Q000378:metabolism; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000187:drug effects / Q000502:physiology
chemical_list: D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D008070:Lipopolysaccharides; D009842:Oligopeptides; D010456:Peptides, Cyclic; D010880:Piperidines; D011506:Proteins; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin; C086539:BQ 788; D010047:Ovalbumin; C072247:cyclo(Trp-Asp-Pro-Val-Leu)