title: Meiotic recombination in RAD54 mutants of Saccharomyces cerevisiae.
abstract: The Rad54 protein is an important component of the recombinational DNA repair pathway in vegetative Saccharomyces cerevisiae cells. Unlike those in other members of the RAD52 group, the meiotic defect in rad54 is rather mild, reducing spore viability only to 26%-65%. A consistently greater requirement for Rad54p during meiosis was observed in hybrid strains, suggesting that Rad54p has a certain role in interhomolog interactions. Such a role is probably minor as no recombination defects were found in the surviving gametes in three genetic intervals on chromosome V. Also, the spore viability pattern in tetrads did not reflect an increase in nondisjunction at meiosis I indicative of a meiotic recombination defect. We suggest that the meiotic defect of rad54 cells lies in the failure to repair meiosis-specific double-strand breaks outside the context of the highly differentiated pathway leading to interhomolog joint molecules and meiotic crossovers that ensure accurate segregation at meiosis I.
authors.affiliation: Institute of General Microbiology, University of Bern, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Schmuckli-Maurer
authors.forename: W D
authors.initials: WD
authors.lastname: Heyer
mesh_terms: D003433:Crosses, Genetic; D004265:DNA Helicases; D004260:DNA Repair; D045643:DNA Repair Enzymes; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008540:Meiosis / Q000235:genetics*; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013172:Spores, Fungal / Q000235:genetics
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C075468:RAD54 protein, S cerevisiae; D004265:DNA Helicases; D045643:DNA Repair Enzymes