Cloning of the gene coding for Staphylococcus hyicus exfoliative toxin B and its expression in Escherichia coli.
The Staphylococcus hyicus exfoliative toxin B (SHETB) gene was cloned into pUC118 and expressed in Escherichia coli. The nucleotide sequence of the SHETB gene consists of a coding region of 804 bp specifying a polypeptide of 268 amino acid residues, which included a putative 20-residue signal sequence.
Watanabe
T
T
Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Sato
H
H
Hatakeyama
Y
Y
Matsuzawa
T
T
Kawai
M
M
Aizawa
C
C
Danbara
H
H
Maehara
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005083:Exfoliatins / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D013210:Staphylococcus / Q000235:genetics* / Q000472:pathogenicity
D005083:Exfoliatins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins