Mice lacking the transcriptional corepressor TIF1beta are defective in early postimplantation development.
TIF1beta, a member of the transcriptional intermediary factor 1 family, has been reported to function as a corepressor for the large class of KRAB domain-containing zinc finger proteins of the Krüppel type. To address the biological function of TIF1beta, we have generated TIF1beta-deficient mice by gene disruption. TIF1beta protein was detected in wild-type but not TIF1beta(-/-) blastocysts. Homozygous mutant embryos, which developed normally until the blastocyst stage and underwent uterine implantation, were arrested in their development at the early egg-cylinder stage at about embryonic day (E) 5.5 and were completely resorbed by E8.5. Taken together, these results provide genetic evidence that TIF1beta is a developmental regulatory protein that exerts function(s) essential for early postimplantation development.
Cammas
F
F
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, BP 163, France.
Mark
M
M
Dollé
P
P
Dierich
A
A
Chambon
P
P
Losson
R
R
D000483:Alleles; D000818:Animals; D001755:Blastocyst / Q000502:physiology; D015153:Blotting, Western; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D004622:Embryo, Mammalian / Q000378:metabolism / Q000502:physiology*; D005326:Fetal Proteins; D005455:Fluorescent Antibody Technique; D005775:Gastrula / Q000502:physiology; D005838:Genotype; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016296:Mutagenesis; D009687:Nuclear Proteins; D010641:Phenotype; D017434:Protein Structure, Tertiary; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D013234:Stem Cells; D020825:T-Box Domain Proteins / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors; D000076183:Tripartite Motif-Containing Protein 28; D016335:Zinc Fingers; D000098908:Brachyury Protein
D004268:DNA-Binding Proteins; D005326:Fetal Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D020825:T-Box Domain Proteins; D014157:Transcription Factors; D000076183:Tripartite Motif-Containing Protein 28; D000098908:Brachyury Protein; C103190:Trim28 protein, mouse