title: Osmotic regulation of cyclic 1,2-beta-glucan synthesis.
abstract: In contrast to what happens in AGROBACTERIUM: tumefaciens and RHIZOBIUM: meliloti, synthesis of periplasmic cyclic 1,2-beta-glucan in BRUCELLA: spp. was not inhibited when bacteria were grown in media of high osmolarity. Studies performed with crude membrane preparations showed that cyclic 1,2-beta-glucan synthetase of BRUCELLA: spp. was not inhibited by 0.5 M KCl or potassium glutamate; concentrations that completely inhibit the osmosensitive enzymes of A. tumefaciens A348 or R. meliloti 102F34, respectively encoded by the chvB or ndvB genes. The BRUCELLA: abortus cyclic 1, 2-beta-glucan synthetase gene (cgs) was introduced into A. tumefaciens A1011 chvB and R. meliloti GRT21s ndvB mutants. Synthesis of cyclic 1,2-beta-glucan by the recombinant strains was not inhibited when grown in media of high osmolarity (0.25 M NaCl or 0.5 M mannitol). On the other hand, when the A. tumefaciens cyclic 1, 2-beta-glucan synthetase gene was introduced into the R. meliloti GRT21s ndvB mutant, the recombinant strain displayed marked inhibition of cyclic 1,2-beta-glucan synthesis when grown in high-osmolarity media. However, the same gene introduced into a B. abortus cgs mutant background resulted in no inhibition of glucan synthesis at high osmolarity. In vitro studies with crude membranes isolated from recombinant strains revealed that BRUCELLA: cyclic 1, 2-beta-glucan synthetase was not inhibited by high concentrations of KCl or potassium glutamate even when expressed in AGROBACTERIUM: or RHIZOBIUM: backgrounds. It was concluded that the lack of effect of high osmolarity on 1,2-beta-glucan synthesis in BRUCELLA: is due to two convergent mechanisms: a) the presence of a cyclic 1, 2-beta-glucan synthetase that is not affected by concentrations of solutes such as KCl or potassium glutamate and b) either the possible accumulation of compatible solutes that might protect the enzyme from the inhibition by potassium glutamate or the accumulation of other osmolytes that do not affect the 1, 2-beta-glucan synthetase.
authors.affiliation: Instituto de Investigaciones Biotecnólogicas, Universidad Nacional de General San Martı́n, Av. General Paz entre Constituyentes y Albarellos, PO Box 30, (1650) Buenos Aires, Argentina1.
authors.forename: Nora Iñón
authors.initials: NI
authors.lastname: de Iannino
authors.affiliation: Instituto de Investigaciones Biotecnólogicas, Universidad Nacional de General San Martı́n, Av. General Paz entre Constituyentes y Albarellos, PO Box 30, (1650) Buenos Aires, Argentina1.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Briones
authors.affiliation: Instituto de Investigaciones Biotecnólogicas, Universidad Nacional de General San Martı́n, Av. General Paz entre Constituyentes y Albarellos, PO Box 30, (1650) Buenos Aires, Argentina1.
authors.forename: Florencia
authors.initials: F
authors.lastname: Iannino
authors.affiliation: Instituto de Investigaciones Biotecnólogicas, Universidad Nacional de General San Martı́n, Av. General Paz entre Constituyentes y Albarellos, PO Box 30, (1650) Buenos Aires, Argentina1.
authors.forename: Rodolfo A
authors.initials: RA
authors.lastname: Ugalde
mesh_terms: D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins; D001622:Betaine / Q000494:pharmacology; D002003:Brucella abortus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003470:Culture Media; D005936:Glucans / Q000032:analysis / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005971:Glutamates / Q000494:pharmacology; D009154:Mutation; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000235:genetics; D047071:beta-Glucans
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D005936:Glucans; D005971:Glutamates; D011994:Recombinant Proteins; D047071:beta-Glucans; D001622:Betaine; D011189:Potassium Chloride; C033744:beta-1,2-glucan; D005964:Glucosyltransferases; C114346:cyclic beta(1-2) glucan synthetase, Brucella melitensis