[Influence of HBV/C mutation on HLA expression of host cells].
OBJECTIVE
To study the influence of HBV/C mutation on host cellular HLA expression.


METHODS
HBV expression vectors carrying wild or variant HBV/C gene were constructed and transferred into HepG(2) cells. HBV/C gene expression on the host cells was identified. The expression of HLA-I and HLA-DR on the host cell membrane was detected.


RESULTS
DNA segments similar with HBV/C gene size and HBcAg were detected by PCR and Western blot analysis, respectively. Almost all HepG(2) cells expressed HLA-I but not HLA-DR. The fluorescence intensity of HLA-I expression on host cells was different: HepG(2) was 57.8 and wild vector 54.3. The wild HBV/C gene declined significantly to 31.2. While V60, G87 and L97 increased to 43.0, 54.0 and 69.4, respectively. L97 was greatly increased with 11.6 higher than HepG(2). HLA-DR had no significant expression.


CONCLUSIONS
Replication and expression of HBV/C gene can influence HLA-I expression on the host cells directly. Influence of variant gene on HLA-I expression is different from the wild gene. This might relate with the exacerbation of diseases resulting from HBV mutation.
Sui
L
L
Department of Infectious Diseases, Nanfang Hospital, Guangzhou 510515, China.
Zhou
F
F
Luo
K
K
D000818:Animals; D002460:Cell Line; D006684:HLA-DR Antigens / Q000032:analysis*; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D051379:Mice; D009154:Mutation
D006684:HLA-DR Antigens; D006512:Hepatitis B Core Antigens; D015395:Histocompatibility Antigens Class I