title: Distribution of aromatase mRNA in the ram hypothalamus: an in situ hybridization study.
abstract: The regional distribution of neurones expressing aromatase mRNA in the ram hypothalamus was examined by in situ hybridization using 33P-labelled cRNA probes. The highest amounts of hybridization signal were observed in the central part of the medial preoptic nucleus and posterior medial part of the bed nucleus of the stria terminalis. Moderate amounts of hybridization signal were observed in the anteroventral periventricular preoptic nucleus, medial preoptic nucleus and a broad band extending between the medial preoptic nucleus and bed nucleus of the stria terminalis. Low levels of hybridization signal were observed in the organum vasculosum of the lamina terminalis, anterior part of the medial preoptic nucleus, and central part of the ventromedial nucleus of the hypothalamus. The presence of aromatase mRNA within neurones of the steroid-sensitive hypothalamic circuit supports a role for aromatization in the mechanism of testosterone action on reproductive function in male sheep. The distribution of aromatase mRNA in the ovine hypothalamus was similar to that described for other vertebrate species, suggesting a high degree of functional conservation across species.
authors.affiliation: Department of Physiology and Pharmacology, School of Medicine, Oregon Health Sciences University, Portland, Oregon 97201-3098, USA. rosellic@ohsu.edu
authors.forename: C E
authors.initials: CE
authors.lastname: Roselli
authors.forename: F
authors.initials: F
authors.lastname: Stormshak
authors.forename: J A
authors.initials: JA
authors.lastname: Resko
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D007031:Hypothalamus / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary; D012333:RNA, Messenger; D001141:Aromatase