Amplification in human breast cancer of a gene encoding a c-myc mRNA-binding protein.
The coding region determinant-binding protein (CRD-BP) binds in vitro to c-myc mRNA and is thought to stabilize the mRNA and increase c-Myc protein abundance. The CRD-BP gene has 15 exons and 14 introns, is single-copy, and is located on chromosome 11 in mice and 17 in humans, close to HER-2/neu. The CRD-BP gene is moderately amplified in 12 of 40 human breast cancers; it is highly amplified in 2 others (14.4 and 20 copies). Despite their proximity, CRD-BP and HER-2/neu genes can be amplified independently. Amplification of a gene that might up-regulate c-Myc abundance could accelerate breast cancer.
Doyle
G A
GA
Department of Oncology, University of Wisconsin-Madison, 53706, USA.
Bourdeau-Heller
J M
JM
Coulthard
S
S
Meisner
L F
LF
Ross
J
J
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D005091:Exons; D005260:Female; D005784:Gene Amplification; D018628:Gene Dosage; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics
D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C406144:IGF2BP1 protein, human