Neuroprotective effect of retro-inverso Prosaptide D5 on focal cerebral ischemia in rat.
Prosaposin (the precursor of saposins A-D) has been identified as a neurotrophic factor in vitro and in vivo. In this study, a novel 11-mer retro-inverso peptidomimetic, Prosaptide D5, was injected i.m. to assess its effectiveness in a rat ischemic model produced by reversible total occlusion of the left middle cerebral artery (MCA). Prosaptide (300 microg/kg, i.m.) injected 3 h after reversible occlusion reduced brain infarct area by 56% compared with a saline group (p < 0.01) at 21 h of reperfusion. A similar injection of D5 6h after occlusion produced a 32% decrease.
Lu
A G
AG
Department of Neurosciences, School of Medicine, Center for Molecular Genetics, University of California at San Diego, La Jolla 92093-0634, USA.
Otero
D A
DA
Hiraiwa
M
M
O'Brien
J S
JS
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D001929:Brain Edema / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology*; D002544:Cerebral Infarction / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics / Q000493:pharmacokinetics / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; C404155:prosaptide D5