title: The stretch-inactivated channel, a vanilloid receptor variant, is expressed in small-diameter sensory neurons in the rat.
abstract: Exposure to hypertonic conditions is known to produce pain and activate small-diameter sensory neurons. Recently, the vanilloid receptor variant and stretch-inactivated ion channel (SIC) was cloned and shown to mediate an inward current in response to cell shrinkage. Since other vanilloid receptors have been previously shown to mediate nociception, we investigated whether SIC is expressed in sensory neurons. Using reverse transcription-polymerase chain reaction and in situ hybridization techniques, we identified SIC in the neurons of dorsal root and trigeminal ganglia. Furthermore, SIC was found to be present almost exclusively in the small-diameter sensory neurons, which includes the nociceptive population. Since SIC is activated by cell shrinkage, it may participate in the mediation of pain produced by hypertonic stimuli.
authors.affiliation: Department of Anesthesia and Perioperative Care, University of California, San Francisco 94143-0648, USA. schumac@itsa.ucsf.edu
authors.forename: M A
authors.initials: MA
authors.lastname: Schumacher
authors.forename: B E
authors.initials: BE
authors.lastname: Jong
authors.forename: S L
authors.initials: SL
authors.lastname: Frey
authors.forename: S P
authors.initials: SP
authors.lastname: Sudanagunta
authors.forename: N F
authors.initials: NF
authors.lastname: Capra
authors.forename: J D
authors.initials: JD
authors.lastname: Levine
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001923:Brain Chemistry / Q000502:physiology; D018076:DNA, Complementary / Q000302:isolation & purification; D005727:Ganglia, Spinal / Q000166:cytology; D015870:Gene Expression / Q000502:physiology; D017403:In Situ Hybridization; D007473:Ion Channels / Q000235:genetics*; D008969:Molecular Sequence Data; D009475:Neurons, Afferent / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D009619:Nociceptors / Q000378:metabolism; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011955:Receptors, Drug / Q000235:genetics*; D013314:Stress, Mechanical
chemical_list: D018076:DNA, Complementary; D007473:Ion Channels; D012333:RNA, Messenger; D011955:Receptors, Drug