A novel HLA-B*40 allele and novel exon 1 sequences of two B*40 alleles identified in potential marrow donors.
Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) typing suggested the presence of variants of HLA-B*40 in three individuals. Two were part of 3,500 potential marrow donors being screened for the National Marrow Donor Program, while the third was a clinical specimen. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. In one individual, a native of the Pacific Islands, sequencing revealed a novel HLA-B*40 allele (B*4023). In two other individuals, a previously unknown exon 1 sequence was determined for HLA-B*4016 (ethnicity unknown) and B*4020 (Hispanic). These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations.
Gourley
I S
IS
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19305, USA.
Wu
J
J
Bassinger
S
S
Montoya
G D
GD
Yee
J
J
Griffith
B B
BB
Kearns
J
J
McKeen
M
M
Birkos
S
S
Williams
T M
TM
Kamoun
M
M
D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005091:Exons / Q000235:genetics*; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012042:Registries; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens