Role of putative loops 2 and 3 in imipenem passage through the specific porin OprD of Pseudomonas aeruginosa.
Mutant proteins with eight amino acid deletions in putative surface loops 2 and 3 of the imipenem-specific porin OprD of Pseudomonas aeruginosa failed to reconstitute imipenem susceptibility in an oprD-deficient background. The loop 3 deletion prevented the ability of imipenem to inhibit KCl conductance through the OprD channel, as previously shown for a loop 2 deletion. This suggests that both loops 2 and 3 have a role in imipenem binding to the OprD channel.
Ochs
M M
MM
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.
Bains
M
M
Hancock
R E
RE
D000595:Amino Acid Sequence; D001692:Biological Transport; D017353:Gene Deletion; D015378:Imipenem / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D018272:Porins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011189:Potassium Chloride / Q000378:metabolism; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013845:Thienamycins / Q000378:metabolism*
D018272:Porins; D013845:Thienamycins; C077157:OprD protein, Pseudomonas aeruginosa; D011189:Potassium Chloride; D015378:Imipenem