title: Characterization of JOK-1, a human gastric epithelial cell line.
abstract: Human gastric epithelial cells were isolated from samples of human gastric lining and immortalized with simian virus 40 (SV40) to generate the stable human gastric epithelial cell line "JOK-l." These cells express conventional epithelial markers (vimentin, cytokeratin-18, occludin, N- and E-cadherins, beta-catenin, ZO-1, ZO-2, mucin, epithelial specific antigen) as well as SV40 large T-antigen. These cells rapidly externalized E-cadherin in response to acidic medium, and exhibited epithelial-like barrier properties that are also regulated by media pH. In contrast, the kidney epithelial cell line "MDCK" also expresses several epithelial markers (vimentin, cytokeratin-18, occludin, N- and E-cadherin, beta-catenin, ZO-1, ZO-2, epithelial specific antigen), but does not express mucin, or large T-antigen. However, MDCK rapidly internalize their E-cadherin from the cell surface and increase the solute flux in an acidic medium. These data suggest that the JOK-1 cell line is a potentially useful cell line for developing models of gastric epithelial function, development, and disease.
authors.affiliation: Department of Molecular and Cellular Physiology Louisiana State University Medical Center, Shreveport, 71130-393, USA.
authors.forename: N
authors.initials: N
authors.lastname: Okayama
authors.forename: M R
authors.initials: MR
authors.lastname: Fowler
authors.forename: S R
authors.initials: SR
authors.lastname: Jennings
authors.forename: R
authors.initials: R
authors.lastname: Specian
authors.forename: B
authors.initials: B
authors.lastname: Alexander
authors.forename: T H
authors.initials: TH
authors.lastname: Jackson
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: T
authors.initials: T
authors.lastname: Shannon
authors.forename: J S
authors.initials: JS
authors.lastname: Alexander
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D002461:Cell Line, Transformed / Q000166:cytology* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005819:Genetic Markers; D006801:Humans; D007633:Keratins / Q000378:metabolism; D007668:Kidney / Q000166:cytology; D008565:Membrane Proteins / Q000378:metabolism; D062793:Occludin; D010750:Phosphoproteins / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D013270:Stomach / Q000166:cytology*; D015534:Trans-Activators; D014162:Transfection; D014746:Vimentin / Q000378:metabolism; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D051176:beta Catenin
chemical_list: D000952:Antigens, Polyomavirus Transforming; C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D005819:Genetic Markers; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; D010750:Phosphoproteins; C569042:TJP1 protein, human; C569046:TJP2 protein, human; D015534:Trans-Activators; D014746:Vimentin; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D051176:beta Catenin; D007633:Keratins