Morphometry of the FRTL-5 cells after irradiation.
The morphological changes of in vitro irradiated FRTL-5 cells and their ability to grow in semi-solid medium were studied morphometrically. FRTL-5 cells were grown in medium with 4 different concentrations of TSH (0, 0.1, 1, 10 mU/ml). After irradiation with 0 Gy, 2 Gy and 4 Gy, the cells were seeded on glass cover-slips and in methocel. Fourteen days after irradiation, the morphometric analysis of FRTL-5 cells and their nuclei was performed. The results showed that irradiation and different doses of TSH have influence on FRTL-5 cell size, more on their nuclei than on the cells as a whole. Growing of FRTL-5 cells in the methocel indicates the possible transformation of these cells after long-culturing in the TSH medium and after irradiation.
Stiblar-Martincic
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Institute of Histology and Embryology, Medical Faculty, University of Ljubliana, Slovenia. Draga.Stiblar@MF.UNI-LJ.SI
Pajer
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Z
Cör
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A
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D051381:Rats; D011916:Rats, Inbred F344; D013961:Thyroid Gland / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects*; D013972:Thyrotropin / Q000494:pharmacology; D014965:X-Rays
D013972:Thyrotropin