title: Sexually dimorphic expression of two types of DM (Doublesex/Mab-3)-domain genes in a teleost fish, the Tilapia (Oreochromis niloticus).
abstract: Sex determination consists of somatic and germ-line sex differentiation hierarchies whose interaction is poorly understood. A single gene known to control somatic sex determination, the DM-domain containing (Doublesex/Mab-3 DNA-binding motif) gene, is highly conserved across species. Vertebrate DMRT1 (DM-related transcription factor 1) expression occurs predominantly in testis. We, however, isolated two distinct DM-domain cDNAs from tilapia testis and ovary, named tDMRT1, and tDMO (DM-domain gene in Ovary), respectively. Despite high homology in the DM-domain, there is little similarity outside the DM-domain. A male specific motif is absent in tDMO indicating a similarity with the female type of doublesex in Drosophila. In contrast to the alternatively spliced male and female types of doublesex, tDMRT1 and tDMO cDNAs are encoded by two different genes. The mutually exclusive nature of tDMRT1 and tDMO expression in the testis or ovary suggests that they both play important roles in gonadal development and/or function.
authors.affiliation: Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Guan
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Nagahama
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics*; D005260:Female; D029941:Fish Proteins; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D012727:Sex Characteristics; D019849:Sex Determination Processes; D013737:Testis / Q000196:embryology / Q000378:metabolism; D017210:Tilapia / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics
chemical_list: C410952:DMO protein, Oreochromis niloticus; C110547:DMRT1 protein; D029941:Fish Proteins; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors