[Investigation of nucleolar organizer region activity in epithelial cells of hyperplastic endometrial carcinoma tissue].
Such characteristics of nucleolar organizer regions (NORs) as nucleolar and intra-nucleolar grain counts, NOR mean area, mean total NOR area in nucleus, nuclear area and nucleolus/nucleus ratio were identified using vaginal aspirate-smears from 34 patients with histologically verified diagnosis (adenocarcinoma--10, atypical hyperplasia (ATH)--10, glandular endometrial hyperplasia (GEH)--4); silver-staining and micro-imaging analyzer techniques were employed. Atypical hyperplasia and nucleolar count in nucleus progressively and significantly increased in the following sequence: proliferative phase--GEH--adenocarcinoma. Mean and total area of NORs showed a significant rise in ATH and adenocarcinoma. Those indices of endometrial cell proliferation and GEH were identical. Nuclear area and nucleolus/nucleus ratio offered most information for differentiating GEH, ATH and carcinoma. A highly reliable correlation was established between intra-nucleolar grain count and microscopically-assayed silver concentration, on the one hand, and NOR area, on the other.
Ellinidi
V N
VN
N.N. Petrov Research Institute of Oncology, Ministry of Health of the RF, St. Petersburg.
Novik
V I
VI
Pozharisskiĭ
K M
KM
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D004714:Endometrial Hyperplasia / Q000378:metabolism / Q000473:pathology*; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000473:pathology*
Issledovanie aktivnosti iadryshkoorganizuiushchikh zon v épitelial'nykh kletkakh pri giperplaziiakh i rake éndometriia s ispol'zovaniem analiza izobrazheniia.