Distribution of HIV-1 subtypes among HIV-seropositive patients in the interior of Côte d'Ivoire.
Limited data exist on the distribution of HIV-1 subtypes in Côte d'Ivoire. The aim of this study is to describe the distribution of genetic subtypes of HIV-1 strains in six regions of Côte d'Ivoire. In 1997, we consecutively collected blood from 172 HIV-1-infected patients from six regional tuberculosis treatment centers. Peripheral blood mononuclear cells (PBMCs) from these people were analyzed by a restriction fragment-length polymorphism (RFLP) assay that involves a sequential endonuclease digestion of a 297-base pair polymerase chain reaction (PCR) fragment; plasma samples were tested by a V3-loop peptide enzyme immunoassay (PEIA). DNA sequencing of the protease or env genes was performed on all samples discordant in the two assays as well as a random sample of the concordant subtyped samples. Of 172 specimens, 3 were PCR-negative, and 169 were putatively classified as subtype A by RFLP. The 3 PCR-negative samples were unequivocally subtyped A by PEIA. Of the 169 RFLP subtype A samples, 159 (94%) were subtyped A by PEIA. Of the 10 discordant samples, PEIA testing classified 3 as subtype C, 2 as D, and 5 as F. Sequencing of the env gene classified these samples as 1 subtype A, 4 Ds, and 5 Gs. Thus, 163 (95%) of the specimens were subtype A, 3 subtype D, 4 subtype G, 1 A/D, and 1 A/G (IbNG) circulating recombinant forms (CRF). In conclusion, most HIV-1-infected tuberculosis patients throughout the interior of Côte d'Ivoire are infected with HIV-1 subtype A, which are very likely the A/G (IbNG) CRF. The uniform distribution of this subtype makes Côte d'Ivoire a potential site for vaccine trials.
Nkengasong
J N
JN
Laboratory of Virology, Project RETRO-CI, Abidjan, Côte d'Ivoire. jcn5@cdc.gov
Luo
C C
CC
Abouya
L
L
Pieniazek
D
D
Maurice
C
C
Sassan-Morokro
M
M
Ellenberger
D
D
Hu
D J
DJ
Pau
C P
CP
Dobbs
T
T
Respess
R
R
Coulibaly
D
D
Coulibaly
I M
IM
Wiktor
S Z
SZ
Greenberg
A E
AE
Rayfield
M
M
D017088:AIDS-Related Opportunistic Infections / Q000097:blood / Q000276:immunology / Q000821:virology*; D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D007560:Cote d'Ivoire; D004279:DNA, Viral; D005260:Female; D015752:Genes, env; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology; D016333:HIV Protease / Q000145:classification / Q000235:genetics*; D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000097:blood / Q000276:immunology / Q000821:virology*
D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D016333:HIV Protease