Suppression by radiosensitizer AK-2123 of early phorbol myristate acetate-stimulated chemiluminescence response of human neutrophils induced by gamma-irradiation (short communication).
The influence of gamma-irradiation and radiosensitizer AK-2123 on secretion of reactive oxygen species (ROS) of human neutrophils was studied. gamma-Irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous chemiluminescence and activated phorbol-12-myristate-13-acetate (PMA)-stimulated chemiluminescence. The time of maximum PMA-stimulated chemiluminescence amplitude was decreased with the dose range from 2 to 25 Gy. The addition of radiosensitizer AK-2123 depressed the early PMA-stimulated chemiluminescence response to gamma-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils and may prevent the radiation-induced damage of blood cells.
Schepetkin
I
I
Oncology Institute of Tomsk Research Centre of Russian Acad Med. Sci., Russia.
D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008163:Luminescent Measurements; D018375:Neutrophil Activation / Q000187:drug effects* / Q000528:radiation effects; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*
D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D013755:Tetradecanoylphorbol Acetate