title: Tissue factor antigen and activity in serum of postoperatively shed blood used for autologous transfusion.
abstract: Orthopaedic surgery involves extensive dissection of connective and richly vascularised tissues rich in tissue factor (TF). It was, therefore, of interest to quantify the amount of TF antigen and activity in postoperatively drained, unwashed wound blood collected for the purpose of autologous transfusion. In nine young patients subjected to surgery for idiopathic thoracic scoliosis, samples were drawn postoperatively from collected shed blood, a pulmonary artery catheter and a radial arterial cannula prior to, during and after reinfusion of shed blood (10 ml/kg body weight), and analysed for TF antigen and activity. Preoperative arterial control samples contained 128 pg/ml TF antigen compared with 40 pg/ml postoperatively. During reinfusion of drained blood, arterial TF concentration rose to 96 pg/ml and dropped to 64 pg/ml after infusion. Arterial and mixed venous blood did not differ significantly in TF levels. Serum from drained blood contained high concentrations of TF antigen (773 pg/ml), but no TF activity was detected. It is concluded that the high concentrations of TF antigen in serum from postoperatively drained blood collected for autologous transfusion are devoid of procoagulant activity. The TF antigen in plasma of drained blood is suggested to be a soluble, proteolysed TF-apoprotein or a TF complex inactivated by the TF pathway inhibitor (TFPI).
authors.affiliation: National Hospital, Centre for Orthopaedics, Oslo, Norway.
authors.forename: C D
authors.initials: CD
authors.lastname: Krohn
authors.forename: O
authors.initials: O
authors.lastname: Reikerås
authors.forename: S
authors.initials: S
authors.lastname: Bjørnsen
authors.forename: F
authors.initials: F
authors.lastname: Brosstad
mesh_terms: D000293:Adolescent; D000328:Adult; D016063:Blood Loss, Surgical; D001804:Blood Transfusion, Autologous; D005260:Female; D006801:Humans; D008297:Male; D009985:Orthopedics; D013925:Thromboplastin / Q000032:analysis*
chemical_list: D013925:Thromboplastin