title: Mechanisms underlying regulated CFTR trafficking.
abstract: Stimulation of membrane capacitance and cell surface labeling of epitope-tagged CFTR provide evidence of cAMP-regulated CFTR trafficking. Co-expression of syntaxin 1A inhibits cAMP-stimulated current and capacitance changes in CFTR expressing cells and blocks cAMP-induced increases in cell surface CFTR. Inhibition of CFTR trafficking by syntaxin over-expression suggests a role for SNARE proteins in this process. CFTR phosphorylation may alter physical interactions with SNARE proteins to regulate plasma membrane CFTR density.
authors.affiliation: Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pennsylvania, USA.
authors.forename: K W
authors.initials: KW
authors.lastname: Peters
authors.forename: J
authors.initials: J
authors.lastname: Qi
authors.forename: S C
authors.initials: SC
authors.lastname: Watkins
authors.forename: R A
authors.initials: RA
authors.lastname: Frizzell
mesh_terms: D000242:Cyclic AMP / Q000502:physiology; D003550:Cystic Fibrosis / Q000235:genetics*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D050765:Qa-SNARE Proteins; D050827:Syntaxin 1
chemical_list: C505032:CFTR protein, human; D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; C494212:STX1A protein, human; D050827:Syntaxin 1; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000242:Cyclic AMP