Micro-electrode techniques for the analysis of oral fluids.
This paper describes the use of micro-electrodes for the analysis of small fluid volumes recovered from the oral environment. The analysis has several advantages: (1) It directly measures the activity of ions, a quantity more relevant to mineral saturation than the conventionally measured concentration, (2) minimum fluid volume for analysis is usually less than 0.005 microL, small enough to avoid sample pooling in most analyses, (3) numerous ions can be measured simultaneously, (4) the analysis time is very short, and (5) the use of mineral oil to isolate specimens provides a simple method for controlling the CO2 tension and humidity over the specimens.
Vogel
G L
GL
Paffenbarger Research Center, American Dental Association Health Foundation, National Bureau of Standards, Gaithersburg, Maryland 20899, USA.
Carey
C M
CM
Chow
L C
LC
Brown
W E
WE
D002118:Calcium / Q000032:analysis; D002245:Carbon Dioxide / Q000737:chemistry; D002712:Chlorides / Q000032:analysis; D003773:Dental Plaque / Q000737:chemistry*; D004867:Equipment Design; D005122:Exudates and Transudates / Q000737:chemistry; D005459:Fluorides / Q000032:analysis; D006801:Humans; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D017736:Ion-Selective Electrodes; D008839:Microelectrodes; D008899:Mineral Oil; D010313:Partial Pressure; D010710:Phosphates / Q000032:analysis; D011188:Potassium / Q000032:analysis; D012463:Saliva / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
D002712:Chlorides; D010710:Phosphates; D002245:Carbon Dioxide; D008899:Mineral Oil; D005459:Fluorides; D011188:Potassium; D002118:Calcium