title: Assessment of insulin sensitivity by 90 min isoglycaemic hyperinsulinaemic glucose clamp in healthy young men.
abstract: We aimed to perform a detailed analysis of the isoglycaemic hyperinsulinaemic glucose clamp in relation to the time spent in performing the procedure, and analysed two series performed by independent investigators on different groups (n = 19 and n = 28) of healthy, young men. We calculated glucose disposal rates (GDR) during 20-min periods at different time points during the clamp. There was no difference in 90- and 120-min GDR when comparing the two series. The differences between 90- and 120-min GDR were (mean +/- SD) 0.48 +/- 1.10 mg/kg/min (p = 0.73) and 0.37 +/- 1.05 mg/kg/min (p = 0.71), respectively. The correlations between 90- and 120-min GDR were 0.94 (p < 0.001) and 0.89 (p < 0.001). Correlations between GDR during the second hour of the clamp and fasting plasma insulin ranged from -0.53 (p = 0.020) to -0.55 (p = 0.016) and from -0.44 (p = 0.020) to -0.54 (p = 0.003), respectively, and did not improve after 60 min of clamping. These data suggest that reliable indices of insulin sensitivity in healthy young men may appear even when the isoglycaemic hyperinsulinaemic clamp procedure is shortened from 120 to 90 min. A shorter procedure is time-effective and less expensive, but may be limited to healthy, young Caucasian men.
authors.affiliation: Department of Cardiology, Ullevaal Hospital, University of Oslo, Norway.
authors.forename: H
authors.initials: H
authors.lastname: Reims
authors.forename: A
authors.initials: A
authors.lastname: Høieggen
authors.forename: E
authors.initials: E
authors.lastname: Fossum
authors.forename: S E
authors.initials: SE
authors.lastname: Kjeldsen
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D005215:Fasting / Q000097:blood; D015309:Glucose Clamp Technique; D006801:Humans; D007328:Insulin / Q000097:blood / Q000502:physiology*; D008297:Male; D012016:Reference Values; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007328:Insulin