title: Condition-dependent presence of beta-lipotropin-like peptide in human keratinocytes.
abstract: This study aimed to characterize the beta-endorphin-immunoreactive material (betaE-IR) detectable in normal human keratinocytes (NHK). The effects of different culturing conditions and UV-irradiation on production of betaE-IR by NHK were assessed by radioimmunoassay and HPLC. All culture systems contained low levels of betaE-IR that was increased in conditioned media after UV-irradiation under certain conditions. NHK grown in nutrient-poor medium contained highest levels of betaE-IR that exhibited beta-lipotropin-like properties after HPLC analysis. The other culturing conditions displayed no authentic betaE-related peptides. Our results indicate that under certain culturing conditions NHK can produce POMC peptides like beta-lipotropin, which can be induced by UV-radiation.
authors.affiliation: Department of Dermatology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands. mwintzen@knmg.nl
authors.forename: M
authors.initials: M
authors.lastname: Wintzen
authors.forename: S B
authors.initials: SB
authors.lastname: Zanello
authors.forename: M F
authors.initials: MF
authors.lastname: Holick
authors.forename: V M
authors.initials: VM
authors.lastname: Wiegant
authors.forename: J P
authors.initials: JP
authors.lastname: Burbach
authors.forename: B J
authors.initials: BJ
authors.lastname: Vermeer
mesh_terms: D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects; D011863:Radioimmunoassay; D014466:Ultraviolet Rays; D008083:beta-Lipotropin / Q000378:metabolism*
chemical_list: D008083:beta-Lipotropin