Interaction of fibrinogen with saphenous vein endothelial cells stimulates tyrosine phosphorylation of cortactin.
Fibrinogen can engage diverse receptors on vascular cells, including ICAM-1. We have investigated the effect of fibrinogen on the tyrosine phosphorylation of cortactin, a cytoskeletal protein in human saphenous vein endothelial cells (HSVECs). Incubation of HSVECs with fibrinogen (0-4 microM) caused a concentration-dependent increase in the tyrosine phosphorylation of cortactin. Fibrinogen (4 microM) and fibrinogen fragment D (4 microM) caused 250% and >450% increase in tyrosine phosphorylation of cortactin respectively, but fibrinogen fragment E (50 kDa form) was ineffective. Preincubation of HSVECs with soluble ICAM-1 attenuated the response to fibrinogen. At physiological concentrations fibrinogen binds to receptors (probably including ICAM-1) on HSVECs, to increase tyrosine phosphorylation of cortactin.
Harley
S L
SL
Department of Vascular Surgery, Imperial College School of Medicine, Charing Cross Hospital, London, UK.
Powell
J T
JT
D002478:Cells, Cultured; D051356:Cortactin; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005338:Fibrin Fibrinogen Degradation Products / Q000494:pharmacology; D005340:Fibrinogen / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D012501:Saphenous Vein / Q000166:cytology; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism
C495868:CTTN protein, human; D051356:Cortactin; D005338:Fibrin Fibrinogen Degradation Products; D008840:Microfilament Proteins; C015214:fibrinogen D fragment; C018543:fibrinogen fragment E; D018799:Intercellular Adhesion Molecule-1; D014443:Tyrosine; D005340:Fibrinogen