Characterization of thin layers of glucose oxidase.
Glucose Oxidase (GOD) has been covalently bound to functionalized glass cover slips. The surface density of immobilized GOD molecules was measured by a method based on the amperometric determination of Flavin Adenine Dinucleotide (FAD). Atomic Force Microscopy (AFM) images, obtained in aqueous solution for the covalently bound enzyme, show a monomolecular layer of the enzyme on a functionalized glass surface. The catalytic constants were measured for the immobilized GOD and compared with those of the free enzyme.
Bortoluzzi
S
S
Department of Biological Chemistry, University of Padova.
Di Paolo
M L
ML
Rigo
A
A
Vianello
F
F
Zennaro
L
L
D005898:Glass; D005949:Glucose Oxidase / Q000737:chemistry*; D013499:Surface Properties
D005949:Glucose Oxidase