Evidence for palytoxin as one of the sheep erythrocyte lytic in lytic factors in crude extracts of ciguateric and non-ciguateric reef fish tissue.
The occurrence of palytoxin or its congener in fish extracts has been presented in this study. The presences of hemolytic factors in fish extracts of Hawaiian reef fish and their implication in ciguatera poisoning have been shown by the sheep erythrocyte assay. By use of the anti-palytoxin inhibition assay with fish extracts and sheep red blood cell (RBC), it was shown that palytoxin was one of the major factors in the lysis of sheep erythrocytes. Ouabain, an antagonist of palytoxin for the Na+/K+ ATPase receptor on RBC, also showed inhibition of sheep RBC lysis by fish extracts. From these results, it was concluded that, in part, palytoxin and other palytoxin-related, hemolysin-like factors in fish extracts were responsible for sheep cell hemolysis.
Wachi
K M
KM
Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822, USA.
Hokama
Y
Y
Haga
L S
LS
Shiraki
A
A
Takenaka
W E
WE
Bignami
G S
GS
Levine
L
L
D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D002922:Ciguatoxins / Q000737:chemistry*; D003064:Cnidarian Venoms; D004912:Erythrocytes / Q000187:drug effects*; D005399:Fishes; D006461:Hemolysis; D066298:In Vitro Techniques; D010042:Ouabain / Q000494:pharmacology; D012756:Sheep; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood; D014020:Tissue Extracts / Q000494:pharmacology; D000095702:Polyether Toxins
D000178:Acrylamides; D002922:Ciguatoxins; D003064:Cnidarian Venoms; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D014020:Tissue Extracts; C010272:palytoxin; D000095702:Polyether Toxins