Extraction and chromatographic separation methods in pharmacokinetic studies of Stobadine--an indole-related antioxidant and free-radical scavenger.
This overview provides comprehensive information on the most relevant results of Stobadine preclinical disposition studies. In order to investigate pharmacokinetic processes of the drug in rats, dogs and in human volunteers, several bioanalytical assays based on radiometric, spectrofluorometric, as well as chromatographic determination methods were developed and implemented. In small laboratory animals, the drug absorption, distribution, metabolism and elimination were investigated by administering 3H-labeled Stobadine. Spectrofluorometry was used alternatively for the determination of cold/unlabeled Stobadine in extracts of biomaterials sampled from larger animal species. The chromatographic separation methods proved, however, to be the most advantageous for determining details of the drug disposition and fate in the body.
Soltés
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L
Institute of Experimental Pharmacology, Slovak Academy of Science, Bratislava, Slovak Republic. exfasolt@savba.savba.sk
Bezek
S
S
Ujházy
E
E
Bauer
V
V
D000818:Animals; D000975:Antioxidants; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002243:Carbolines / Q000302:isolation & purification* / Q000493:pharmacokinetics*; D002845:Chromatography / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004285:Dogs; D016166:Free Radical Scavengers; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D013050:Spectrometry, Fluorescence; D014316:Tritium
D000975:Antioxidants; D002243:Carbolines; D016166:Free Radical Scavengers; D014316:Tritium; C100106:dicarbine