title: Xplore mRNA assays for the quantification of IL-1 beta and TNF-alpha mRNA in lipopolysaccharide-induced mouse macrophages.
abstract: Because the accurate measurement of a number of cytokine mRNA transcripts provides valuable knowledge about cytokine gene regulation, we have developed the Xplore assay for the quantification of cytokine mRNA. This microplate-based assay is rapid (under four hours), quantitative over three orders of magnitude and carries no risk of false-positive values from contamination with amplified target. Here, we describe the use of Xplore assays to measure the steady-state mRNA levels of TNF-alpha and IL-1 beta produced by mouse WEHI and J774 macrophage-like cell lines.
authors.affiliation: Endogen, Inc., Woburn, MA, USA.
authors.forename: S W
authors.initials: SW
authors.lastname: Van Arsdell
authors.forename: K P
authors.initials: KP
authors.lastname: Murphy
authors.forename: C
authors.initials: C
authors.lastname: Pazmany
authors.forename: D
authors.initials: D
authors.lastname: Erickson
authors.forename: C
authors.initials: C
authors.lastname: Burns
authors.forename: M D
authors.initials: MD
authors.lastname: Moody
mesh_terms: D000818:Animals; D002460:Cell Line; D007375:Interleukin-1 / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha