Immunohistochemical and chemical changes of beta-citryl-L-glutamate in the differentiation of bovine lens epithelial cells into lens fiber cells.
Beta-citryl-L-glutamate (beta-CG) concentration was determined by HPLC during the differentiation of bovine lens epithelial cells into lens fiber cells in culture. beta-CG increased from 1 to 4 weeks of culture and then decreased slightly, while alpha-crystallin, a marker of lens cell differentiation, increased rapidly 4 weeks after the culture and continued to increase gradually until week 11. In addition, the localization of beta-CG was immunohistochemically examined using anti-beta-CG antibody. Cells around lentoid bodies were stained with anti-beta-CG antibody, whereas cells in the bodies were stained strongly with anti-gamma-crystallin antibody. These findings suggest that beta-CG accumulated immediately before the differentiation of the bovine lens epithelial cells into lens fiber cells and may play a role in regulating the differentiation of lens cells.
Narahara
M
M
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Kobe, Japan. narahara@pharm.kobegakuin.ac.jp
Tachibana
K
K
Kurisu
N
N
Kanazawa
M
M
Miyake
M
M
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005971:Glutamates / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*
D005971:Glutamates; C018491:beta-citrylglutamic acid