Identification of Ehrlichia chaffeensis by nested PCR in ticks from Southern China.
A total of 717 ticks collected from southern China were examined by nested PCR for the presence of Ehrlichia chaffeensis. Sixteen (55. 2%) of 29 adult Amblyomma testudinarium ticks and 28 (11.7%) of 240 adult and at least 4.2% of 215 nymphal (pooled specimens) Haemaphysalis yeni ticks tested positive. Four other species of ticks were negative. Selected positive amplicons were confirmed by DNA sequencing.
Cao
W C
WC
Institute of Microbiology and Epidemiology, Beijing 100071, China. caowc@nic.bmi.ac.cn
Gao
Y M
YM
Zhang
P H
PH
Zhang
X T
XT
Dai
Q H
QH
Dumler
J S
JS
Fang
L Q
LQ
Yang
H
H
D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D002681:China; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018469:Ehrlichia chaffeensis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009758:Nymph / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013987:Ticks / Q000382:microbiology*
D004269:DNA, Bacterial