Bacterial mode of replication with eukaryotic-like machinery in a hyperthermophilic archaeon.
Despite a rapid increase in the amount of available archaeal sequence information, little is known about the duplication of genetic material in the third domain of life. We identified a single origin of bidirectional replication in Pyrococcus abyssi by means of in silico analyses of cumulative oligomer skew and the identification of an early replicating chromosomal segment. The replication origin in three Pyrococcus species was found to be highly conserved, and several eukaryotic-like DNA replication genes were clustered around it. As in Bacteria, the chromosomal region containing the replication terminus was a hot spot of genome shuffling. Thus, although bacterial and archaeal replication proteins differ profoundly, they are used to replicate chromosomes in a similar manner in both prokaryotic domains.
Myllykallio
H
H
Institut de Génétique et Microbiologie, Laboratoire de Biologie Cellulaire, Université de Paris-Sud, 91405 Orsay Cedex, France.
Lopez
P
P
López-García
P
P
Heilig
R
R
Saurin
W
W
Zivanovic
Y
Y
Philippe
H
H
Forterre
P
P
D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D019847:Chromosomes, Archaeal / Q000378:metabolism*; D017124:Conserved Sequence; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics*; D019641:DNA, Archaeal / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism; D019143:Evolution, Molecular; D019766:Genes, Archaeal; D020745:Genome, Archaeal; D051738:Origin Recognition Complex; D011691:Puromycin / Q000494:pharmacology; D019711:Pyrococcus / Q000235:genetics* / Q000378:metabolism*; D018741:Replication Origin; D029701:Saccharomyces cerevisiae Proteins
D019843:Archaeal Proteins; C066503:CDC6 protein, S cerevisiae; D018797:Cell Cycle Proteins; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins; D051738:Origin Recognition Complex; D029701:Saccharomyces cerevisiae Proteins; D011691:Puromycin; D004265:DNA Helicases; C403162:MCM protein, Methanobacterium thermoautotrophicum