Matrix-assisted laser desorption/ionization mass spectrometric quantification of the mu opioid receptor agonist DAMGO in ovine plasma.
The synthetic opioid peptide analog Tyr-D-Ala-Gly-N-methyl-Phe-Gly-ol (DAMGO), which is a mu opioid receptor-selective agonist, was quantified in ovine plasma samples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), using delayed extraction and a reflectron. The internal standard was pentadeuterated DAMGO. Timed-ion selection was used to select the precursor ion. The analysis of the post-source decay fragments improved the detection sensitivity, and the use of the precursor-product ion relationship optimized the specificity. For plasma samples, the inter-assay variability of this method was 6.4% (n = 79) and the intra-assay variability was 6.0% (n = 10). The variability for controls was 3.4% (n = 43). The profile of DAMGO amount versus time was determined in sheep plasma, and the corresponding pharmacokinetic data were calculated.
Desiderio
D M
DM
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, Departments of Neurology and Biochemistry, University of Tennessee, Memphis, 847 Monroe Avenue, Memphis, Tennessee 38163, USA. ddesiderio@utmem.edu
Wirth
U
U
Lovelace
J L
JL
Fridland
G
G
Umstot
E S
ES
Nguyen
T M
TM
Schiller
P W
PW
Szeto
H S
HS
Clapp
J F
JF
D000704:Analysis of Variance; D000818:Animals; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D007262:Infusions, Intravenous; D017450:Receptors, Opioid, mu / Q000819:agonists*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data
D017450:Receptors, Opioid, mu; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-