[Introduction of rabbit defensin NP-1 gene into poplar (P. tomentosa) by Agrobacterium-mediated transformation].
Rabbit defensin NP-1 possesses a broad resistant spectrum to pathogens. In this work, we have transferred the NP-1 gene into poplar plants by Agrobacterium-mediated transformation. PCR amplification and Southern analysis showed that rabbit defensin NP-1 gene was integrated into the poplar genome. The transformation efficiency is about 15.6%. Antimicrobial activity test showed that the extract of transgenic plants inhibited the growth of the tested microbes.
Zhao
S M
SM
Institute of Genetics, Chines Academy of Sciences, Beijing.
Zu
G C
GC
Liu
G Q
GQ
Huang
M R
MR
Xu
J X
JX
Sun
Y R
YR
D000818:Animals; D023082:Defensins; D018014:Gene Transfer Techniques; D030821:Plants, Genetically Modified; D011506:Proteins / Q000235:genetics*; D011817:Rabbits; D012231:Rhizobium / Q000235:genetics*
D023082:Defensins; D011506:Proteins