[The subcellular mechanisms of the action of disinfectants. The effect of iodine and chloramine-B on bacterial ribosomes].
The composition of ribosomal material in bacterial cells of 5 families, subjected to the minimal bactericidal action of iodine and chloramine-B, was studied with the use of electrophoresis in polyacrylamide gel. The study demonstrated that under these conditions protein-synthesizing organelles of vibrios and bifidobacteria did not undergo perceptible changes, while Escherichia, Pseudomonas and bacilli essential ribosomal degradation was found to occur, which was manifested by a decrease in the intensity of bands 23S and 16S of ribosomal RNA. The conclusion was made that the in vivo destruction of ribosomes was not the direct result of the action of disinfectants, but mediated by some cellular mechanisms.
Shandala
M G
MG
Research Institute of Disinfectology, Moscow, Russia.
Lishchenko
N N
NN
D002700:Chloramines / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000187:drug effects*; D007455:Iodine / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D012329:RNA, Bacterial / Q000032:analysis / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000187:drug effects; D012338:RNA, Ribosomal, 23S / Q000032:analysis / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects*; D000096926:Benzenesulfonamides
D002700:Chloramines; D004202:Disinfectants; D007455:Iodine; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; C030263:chloramine-B; D000096926:Benzenesulfonamides
Subkletochnye mekhanizmy deĭstviia dezinfektantov. Vliianie ĭoda i khloramina-B na ribosomy bakteriĭ.