title: Phytanyl-pyrophosphate-linked substrate for a bacterial alpha-mannosyltransferase.
abstract: The biochemical characterization of bacterial glycosyltransferases involved in the assembly of cell-wall-associated polysaccharides is often hindered by the lack of the appropriate undecaprenyl-pyrophosphate-linked acceptor substrate. In order to find a suitable synthetic substrate for the alpha1,3-mannosyltransferase AceA from Acetobacter xylinum, phytanyl-pyrophosphate-linked cellobiose was prepared. In the presence of GDP-[14C]mannose and recombinant AceA, the phytanyl-pyrophosphate-linked cellobiose afforded a 14C-labeled trisaccharide that was sensitive to alpha-mannosidase degradation in a fashion analogous to the natural undecaprenyl-pyrophosphate-linked cellobiose substrate. These results suggest that phytanyl-pyrophosphate-linked oligosaccharides may be useful substrates for other important bacterial glycosyltransferases.
authors.affiliation: CNRS Centre de Recherches sur les Macromolecules Vegetales, associe avec l'Universite Joseph Fourier, Grenoble, France. lellouch@cermav.cnrs.fr
authors.forename: A C
authors.initials: AC
authors.lastname: Lellouch
authors.forename: G M
authors.initials: GM
authors.lastname: Watt
authors.forename: R A
authors.initials: RA
authors.lastname: Geremia
authors.forename: S L
authors.initials: SL
authors.lastname: Flitsch
mesh_terms: D000091:Acetobacter / Q000201:enzymology; D002475:Cellobiose / Q000737:chemistry / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D015394:Molecular Structure; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D011104:Polyisoprenyl Phosphate Oligosaccharides; D011994:Recombinant Proteins; D002475:Cellobiose; C100893:GDP-mannose - cellobiosyl-diphosphopolyprenol mannosyltransferase; D008364:Mannosyltransferases