Profiling of genes which are differentially expressed in mouse liver in response to adenoviral vectors and delivered genes.
The effects of transgene delivery by adenoviral vectors were studied by probing a 588 gene, mouse cDNA array with mRNA derived from infected liver. The liver tissues were obtained from naive mice and mice infected with replication-deficient adenovirus, adenovirus expressing transforming growth factor beta1 (TGFbeta1), and adenovirus expressing connective tissue growth factor (CTGF). Expression of 98 genes was detected in the array analysis. The increased expression of the transcripts for Stat1, gamma interferon-induced monokine (MIG) and interferon regulatory factor 1 (IRF1) clearly demonstrated the immune response induced by infection with a first generation, replication-incompetent adenovirus. In vivo expression of TGFbeta1 led to a down-regulation of genes involved in the immune response. The increased expression of u-PAR1, laminin receptor and BMP-1 confirms the importance of CTGF and TGFbeta1 in angiogenesis, and tissue repair. Expression of the serine protease inhibitors, Spi 2.4 and Spi 2, is also increased in response to AdTGFbeta1 and AdCTGF.
Haberberger
T C
TC
Department of Molecular Technologies, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94710, USA.
Kupfer
K
K
Murphy
J E
JE
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern / Q000379:methods; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors / Q000008:administration & dosage*; D006524:Hepatitis, Viral, Animal / Q000276:immunology*; D050856:Interferon Regulatory Factor-1; D008099:Liver / Q000276:immunology* / Q000821:virology; D008666:Metalloendopeptidases / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics; D017474:Receptors, Laminin / Q000235:genetics; D050794:STAT1 Transcription Factor; D015534:Trans-Activators / Q000235:genetics; D014162:Transfection / Q000379:methods; D016212:Transforming Growth Factor beta / Q000235:genetics*
D019485:Bone Morphogenetic Proteins; D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; C494258:Irf1 protein, mouse; D010455:Peptides; D010750:Phosphoproteins; D017474:Receptors, Laminin; D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; C009862:connective tissue-activating peptide; D008666:Metalloendopeptidases; C525707:Bmp1 protein, mouse; D055395:Bone Morphogenetic Protein 1