title: Cloning and sequence analysis of the Candida boidinii ADE2 gene.
abstract: Candida boidinii ADE2 gene (phosphoribosyl-5-aminoimidazole carboxylase; AIRC, EC 4. 1. 1. 21) has been cloned by homology to the Saccharomyces cerevisiae ADE2 gene. The cloned C. boidinii ADE2 gene complemented the ade2 mutation of S. cerevisiae. Sequence analysis showed a single open reading frame of 1719 nucleotides coding for a polypeptide of 573 residues. Comparison of the deduced amino acid sequence with those of AIRC enzymes from other yeasts showed marked homology among yeast AIRCs.
authors.affiliation: Central Laboratories for Key Technology, Kirin Brewery Co. Ltd., 1-13-5, Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa, 236-0004, Japan. t-komeda@kirin.co.jp
authors.forename: T
authors.initials: T
authors.lastname: Komeda
authors.forename: H
authors.initials: H
authors.lastname: Suda
authors.forename: Y
authors.initials: Y
authors.lastname: Sakai
authors.forename: N
authors.initials: N
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Tamai
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D002262:Carboxy-Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D017353:Gene Deletion; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase