Functionalized nucleoside 5'-triphosphates for in vitro selection of new catalytic ribonucleic acids.
A series of novel 2'-modified nucleoside 5'-triphosphates was synthesized. The amino, imidazole, and carboxylate functionalities were attached to the 5-position of pyrimidine base of these molecules through alkynyl and alkyl spacers, respectively. Two different phosphorylation methods were used to optimize the yields of these highly modified triphosphates.
Matulic-Adamic
J
J
Department of Chemistry & Biochemistry, Ribozyme Pharmaceuticals, Inc., Boulder, CO 80301, USA.
Daniher
A T
AT
Karpeisky
A
A
Haeberli
P
P
Sweedler
D
D
Beigelman
L
L
D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009711:Nucleotides; D016337:RNA, Catalytic