The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus 'Sir Winston Churchill'.
An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.
Ingkaninan
K
K
Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
Hazekamp
A
A
de Best
C M
CM
Irth
H
H
Tjaden
U R
UR
van der Heijden
R
R
van der Greef
J
J
Verpoorte
R
R
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D047151:Amaryllidaceae Alkaloids; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005702:Galantamine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010944:Plants / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D002800:Cholinesterase Inhibitors; C412690:ungiminorine; D005702:Galantamine