title: Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable.
abstract: DNA-based typing of HLA class I alleles of the HLA-A and HLA-B loci using sequence-specific oligonucleotide primers and/or probes has been used for the large-scale typing of individuals for the National Marrow Donor Program unrelated donor registry. Typing was performed by 16 laboratories at a low level of resolution (e.g. A*01, B*07). The results of blinded quality control analysis for the first 12 months of the project show the typing to be highly accurate, specific and reliable. The total error rate based on 11,545 HLA-A and 11,428 HLA-B assignments was 1.1% for HLA-A and 1.9% for HLA-B. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 64,180 donor samples tested at the same time by the laboratories.
authors.affiliation: Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC 20007, USA. hurley@gunet.georgetown.edu
authors.forename: C K
authors.initials: CK
authors.lastname: Hurley
authors.forename: M
authors.initials: M
authors.lastname: Maiers
authors.forename: J
authors.initials: J
authors.lastname: Ng
authors.forename: D
authors.initials: D
authors.lastname: Wagage
authors.forename: J
authors.initials: J
authors.lastname: Hegland
authors.forename: J
authors.initials: J
authors.lastname: Baisch
authors.forename: R
authors.initials: R
authors.lastname: Endres
authors.forename: M
authors.initials: M
authors.lastname: Fernandez-Vina
authors.forename: U
authors.initials: U
authors.lastname: Heine
authors.forename: S
authors.initials: S
authors.lastname: Hsu
authors.forename: M
authors.initials: M
authors.lastname: Kamoun
authors.forename: Y
authors.initials: Y
authors.lastname: Mitsuishi
authors.forename: D
authors.initials: D
authors.lastname: Monos
authors.forename: H
authors.initials: H
authors.lastname: Noreen
authors.forename: L
authors.initials: L
authors.lastname: Perlee
authors.forename: S
authors.initials: S
authors.lastname: Rodriguez-Marino
authors.forename: A
authors.initials: A
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Stastny
authors.forename: M
authors.initials: M
authors.lastname: Trucco
authors.forename: S Y
authors.initials: SY
authors.lastname: Yang
authors.forename: N
authors.initials: N
authors.lastname: Yu
authors.forename: R
authors.initials: R
authors.lastname: Holsten
authors.forename: R J
authors.initials: RJ
authors.lastname: Hartzman
authors.forename: M
authors.initials: M
authors.lastname: Setterholm
mesh_terms: D016026:Bone Marrow Transplantation / Q000276:immunology; D017931:DNA Primers; D005820:Genetic Testing / Q000592:standards; D015234:HLA-A Antigens / Q000032:analysis* / Q000235:genetics*; D015235:HLA-B Antigens / Q000032:analysis* / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods / Q000592:standards*; D006801:Humans; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012042:Registries; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D015234:HLA-A Antigens; D015235:HLA-B Antigens