Ly-6I, a new member of the murine Ly-6 superfamily with a distinct pattern of expression.
A new member of the mouse Ly-6SF, designated Ly-6I, has been isolated as a gene homologous to a segment of the Ly-6C gene. A single allelic difference in the mature protein sequence was identified, which is similar to other Ly-6SF members. Ly-6I mRNA has been detected in a wide range of tissues and cell lines, and a rabbit polyclonal Ab has been used to determine that Ly-6I protein is present at a low constitutive level on cell lines from several different lineages. In contrast to Ly-6C and Ly-6A/E, the Ly-6I gene is only weakly responsive to IFNs. Expression in vivo is most abundant on bone marrow populations and is coexpressed with Ly-6C on granulocytes and macrophages. However, Ly-6I is also expressed on immature B cell populations that do not express Ly-6C. Expression on mature B cells in spleen is uniformly low. Similarly, Ly-6I is expressed on TCRlow/int, but not TCRhigh, thymocytes. Ly-6I is re-expressed on Ly-6Chigh T cells in the periphery. Thus, Ly-6I may be a useful marker to define maturation stages of both T and B lymphocytes as well as subsets of monocytes and granulocytes.
Pflugh
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Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
Maher
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SE
Bothwell
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D016475:3T3 Cells; D000818:Animals; D000950:Antigens, Ly / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry; D005786:Gene Expression Regulation / Q000276:immunology*; D006098:Granulocytes / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D009000:Monocytes / Q000276:immunology / Q000378:metabolism; D005810:Multigene Family / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000950:Antigens, Ly; D018076:DNA, Complementary; C074650:Ly6a protein, mouse; C499392:Ly6i protein, mouse; D008565:Membrane Proteins