title: Isolation of Bartonella henselae from a serologically negative cat in Bloemfontein, South Africa.
abstract: Sera collected from apparently healthy 6-12-month-old cats (n = 31) presented to the Society for the Prevention of Cruelty to Animals Veterinary Clinic in Bloemfontein for neutering were tested for antibodies reactive to Bartonella henselae (Houston-1 strain) by indirect fluorescent antibody testing. Whole blood collected from the cats was used in isolation experiments and subsequent identification of Bartonella species was based on comparison of the nucleotide base sequence of polymerase chain reaction-amplified citrate synthase gene fragments. While none of the cats had antibodies reactive with B. henselae at titres > or =1/64, an organism with a partial citrate synthase gene sequence identical to that of B. henselae (Houston-1) was isolated from 1 cat.
authors.affiliation: Department of Medical Microbiology, Faculty of Health Sciences, University of the Orange Free State, Bloemfontein, South Africa.
authors.forename: A M
authors.initials: AM
authors.lastname: Pretorius
authors.forename: P J
authors.initials: PJ
authors.lastname: Kelly
authors.forename: R J
authors.initials: RJ
authors.lastname: Birtles
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D018416:Bartonella henselae / Q000276:immunology / Q000302:isolation & purification*; D002415:Cats / Q000097:blood / Q000821:virology*; D019084:Fluorescent Antibody Technique, Indirect; D016133:Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D013019:South Africa
chemical_list: D000907:Antibodies, Bacterial