Immunodetection of adenylyl cyclase protein in tissues.
Immunodetection of the adenylyl cyclase isoforms has been difficult in tissues because of its low quantity of protein expression. We have developed a one-step cellular protein purification method that enables a simple immunodetection of the adenylyl cyclase isoforms. The type I isoform was detected exclusively in the brain. The type III isoform was detected in the brain and lungs. Further, the protein expression of type III adenylyl cyclase in lungs changed ontogenically and was the lowest in neonates. Thus, the comparison of the amount of certain adenylyl cyclase isoforms protein in each tissue is now feasible using our method.
Ishikawa
Y
Y
Weis Center for Research, Department of Molecular and Cellular Physiology, Pennsylvania State Medical College, Danville 17822, USA. yishikawa@psghs.edu
Grant
B S
BS
Okumura
S
S
Schwencke
C
C
Yamamoto
M
M
D000262:Adenylyl Cyclases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology; D007150:Immunohistochemistry / Q000379:methods*; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008168:Lung / Q000201:enzymology / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013552:Swine; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D000262:Adenylyl Cyclases