Inhibitory effects of human neutrophil functions by the 45-kD glycoprotein derived from the parasitic nematode Trichinella spiralis.
OBJECTIVE
We evaluated the effect of the 45-kD protein of Trichinella spiralis (gp45), purified by affinity chromatography, on random migration and chemotaxis, the oxidative metabolism of human neutrophils and on the CD11b upregulation induced by formyl-methionyl-leucyl-phenylalanine (f-MLP).


METHODS
Donor neutrophils incubated with different amounts of gp45 (0.5, 1, 1.5, 2 microg/ml) or buffer and the random migration and chemotaxis, evaluated by means of a special technique of image analysis, and the chemiluminescence response to f-MLP or phorbol myristate acetate (PMA) were analyzed. The effect on CD11b upregulation was assessed incubating cells with the protein, when activating them with f-MLP.


RESULTS
The results showed that gp45 inhibited both random and stimulated migrations, and reduced the response to f-MLP and PMA. Furthermore, gp45 significantly reduced the upregulation of the CD11b induced by f-MLP.


CONCLUSIONS
The results show that gp45 inhibits PMN in different functions, suggesting an anti-inflammatory action.
Bruschi
F
F
Istituto di Patologia Generale, Università di Perugia, Italy. f.bruschi@med.unipi.it
Carulli
G
G
Azzarà
A
A
Homan
W
W
Minnucci
S
S
Rizzuti-Gullaci
A
A
Sbrana
S
S
Angiolini
C
C
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism / Q000494:pharmacology*; D015801:Helminth Proteins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D011485:Protein Binding; D017160:Trichinella spiralis / Q000276:immunology*; D015854:Up-Regulation
D000893:Anti-Inflammatory Agents; D006023:Glycoproteins; D015801:Helminth Proteins; D016177:Macrophage-1 Antigen; C411064:glycoprotein 45-kDa, Trichinella; D009240:N-Formylmethionine Leucyl-Phenylalanine