title: Langerhans cells that have matured in vivo in the absence of T cells are fully capable of inducing a helper CD4 as well as a cytotoxic CD8 response.
abstract: Langerhans cells (LCs) are immature dendritic cells (DCs) present in the skin epithelium. Upon Ag exposure, they migrate to the draining lymph nodes where they mature into potent stimulators of naive T cells. The aim of this study was to investigate the influence of T cells on LC migration and maturation. Therefore, the in vivo migration and maturation of LCs after sensitization with the hapten FITC was compared between C57BL/6 or BALB/c mice used as positive controls, and recombination activating gene (RAG) 1 knockout (-/-) mice or SCID mice used as T cell-deficient mice. Phenotypically, there was no difference between migrated LCs from RAG1-/- or SCID mice vs normal C57BL/6 or BALB/c mice: both populations of FITC+ cells had a dendritic morphology and a mature phenotype as they expressed high levels of MHC class II molecules and costimulatory molecules CD80, CD86, and CD54. Sorted migrated LCs of RAG1-/- or SCID mice were efficient stimulators of allogeneic T cells and Ag-specific CD4+ T cells. The same results were found if migrated LCs were fixed instead of irradiated, excluding the possibility that LCs derived from RAG1-/- or SCID mice would mature in the presence of T cells during the stimulation tests. Importantly, fixed migrated LCs of RAG1-/- mice were also efficient stimulators of cytotoxic CD8+ T cells. These data suggest that T cells are not required for full maturation of LCs.
authors.affiliation: Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University Hospital, Ghent, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: De Creus
authors.forename: K
authors.initials: K
authors.lastname: Van Beneden
authors.forename: T
authors.initials: T
authors.lastname: Taghon
authors.forename: F
authors.initials: F
authors.lastname: Stolz
authors.forename: V
authors.initials: V
authors.lastname: Debacker
authors.forename: J
authors.initials: J
authors.lastname: Plum
authors.forename: G
authors.initials: G
authors.lastname: Leclercq
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology; D018398:Homeodomain Proteins / Q000235:genetics; D007114:Immunization; D016130:Immunophenotyping; D007801:Langerhans Cells / Q000166:cytology* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000235:genetics; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D009924:Organ Culture Techniques; D012867:Skin / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D019895:Transposases / Q000235:genetics
chemical_list: D006241:Haptens; D018398:Homeodomain Proteins; C064658:RAG-1 protein; D019895:Transposases; D016650:Fluorescein-5-isothiocyanate