Upregulation of P53 protein in rat heart subjected to a transient occlusion of the coronary artery followed by reperfusion.
The accumulation of p53 protein in cardiomyocyte nuclei was immunohistochemically demonstrated by the pronounced staining of p53 antibody in 4 h-reperfused ventricular tissue after a 45-min coronary occlusion. The occurrence of apoptosis in the reperfused ventricular tissue was evidenced by positive TUNEL staining and DNA laddering on agarose gels.
Xie
Z
Z
3rd Department of Anatomy, School of Medicine, Hokkaido University, Sapporo, 064-0821 Japan.
Koyama
T
T
Abe
K
K
Fuji
Y
Y
Sawa
H
H
Nagtashima
K
K
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D003326:Coronary Circulation / Q000502:physiology*; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D020287:In Situ Nick-End Labeling; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000473:pathology; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53