Characterization of JOK-1, a human gastric epithelial cell line.
Human gastric epithelial cells were isolated from samples of human gastric lining and immortalized with simian virus 40 (SV40) to generate the stable human gastric epithelial cell line "JOK-l." These cells express conventional epithelial markers (vimentin, cytokeratin-18, occludin, N- and E-cadherins, beta-catenin, ZO-1, ZO-2, mucin, epithelial specific antigen) as well as SV40 large T-antigen. These cells rapidly externalized E-cadherin in response to acidic medium, and exhibited epithelial-like barrier properties that are also regulated by media pH. In contrast, the kidney epithelial cell line "MDCK" also expresses several epithelial markers (vimentin, cytokeratin-18, occludin, N- and E-cadherin, beta-catenin, ZO-1, ZO-2, epithelial specific antigen), but does not express mucin, or large T-antigen. However, MDCK rapidly internalize their E-cadherin from the cell surface and increase the solute flux in an acidic medium. These data suggest that the JOK-1 cell line is a potentially useful cell line for developing models of gastric epithelial function, development, and disease.
Okayama
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N
Department of Molecular and Cellular Physiology Louisiana State University Medical Center, Shreveport, 71130-393, USA.
Fowler
M R
MR
Jennings
S R
SR
Specian
R
R
Alexander
B
B
Jackson
T H
TH
Oshima
T
T
Shannon
T
T
Alexander
J S
JS
D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D002461:Cell Line, Transformed / Q000166:cytology* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005819:Genetic Markers; D006801:Humans; D007633:Keratins / Q000378:metabolism; D007668:Kidney / Q000166:cytology; D008565:Membrane Proteins / Q000378:metabolism; D062793:Occludin; D010750:Phosphoproteins / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D013270:Stomach / Q000166:cytology*; D015534:Trans-Activators; D014162:Transfection; D014746:Vimentin / Q000378:metabolism; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D051176:beta Catenin
D000952:Antigens, Polyomavirus Transforming; C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D005819:Genetic Markers; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; D010750:Phosphoproteins; C569042:TJP1 protein, human; C569046:TJP2 protein, human; D015534:Trans-Activators; D014746:Vimentin; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D051176:beta Catenin; D007633:Keratins