title: Rapid in silico cloning of genes using expressed sequence tags (ESTs).
abstract: Expressed sequence tags (ESTs) are short single-pass DNA sequences obtained from either end of cDNA clones. These ESTs are derived from a vast number of cDNA libraries obtained from different species. Human ESTs are the bulk of the data and have been widely used to identify new members of gene families, as markers on the human chromosomes, to discover polymorphism sites and to compare expression patterns in different tissues or pathologies states. Information strategies have been devised to query EST databases. Since most of the analysis is performed with a computer, the term "in silico" strategy has been coined. In this chapter we will review the current status of EST databases, the pros and cons of EST-type data and describe possible strategies to retrieve meaningful information.
authors.affiliation: Glaxo-Wellcome, Genome Informatics, Genetics Division, Herts, England.
authors.forename: R W
authors.initials: RW
authors.lastname: Gill
authors.forename: P
authors.initials: P
authors.lastname: Sanseau
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017124:Conserved Sequence; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D030342:Genetic Diseases, Inborn / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012984:Software