Effect of leukocyte-endothelial adhesion antagonism on neutrophil migration and neurologic outcome after cortical trauma.
BACKGROUND
Administration of anti-CD11B, a monoclonal antibody directed against the leukocyte adhesion molecule CD11B, results in decreased neutrophil infiltration into injured tissue after experimental ischemia. We determined the effect of anti-CD11B administration on neutrophil migration and neurologic functioning after experimental cortical trauma.


METHODS
Injuries were produced by a pneumatic impactor. Treatment animals received anti-CD11B after injury. Neurologic functioning was quantitated at 1, 12, and 24 hours after injury. Neutrophil migration was assessed with the myeloperoxidase assay.


RESULTS
Neutrophil influx was increased in injured cortex after trauma. Anti-CD11B significantly reduced neutrophil influx. There was no significant improvement in neurologic functioning after MAb administration.


CONCLUSIONS
These results show there is marked neutrophil response to injury as produced with the pneumatic contusion model. This migration may be significantly attenuated by administration of a anti-CD11B.
Weaver
K D
KD
University of North Carolina-Chapel Hill Division of Neurosurgery, 27599-7060, USA.
Branch
C A
CA
Hernandez
L
L
Miller
C H
CH
Quattrocchi
K B
KB
D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology / Q000627:therapeutic use*; D001930:Brain Injuries / Q000188:drug therapy* / Q000276:immunology; D002464:Cell Migration Inhibition; D019040:E-Selectin / Q000187:drug effects*; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009460:Neurologic Examination; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000911:Antibodies, Monoclonal; D019040:E-Selectin