Calcium-binding proteins calbindin D28K, calretinin, and parvalbumin immunoreactivity in the rabbit visual cortex.
The distribution and morphology of neurons containing three calcium-binding proteins, calbindin D28K, calretinin, and parvalbumin in the adult rabbit visual cortex were studied. The calcium-binding proteins were identified using antibody immunocytochemistry. Calbindin D28K-immunoreactive (IR) neurons were located throughout the cortical layers with the highest density in layer V. However, calbindin D28K-IR neurons were rarely encountered in layer I. Calretinin-IR neurons were mainly located in layers II and III. Considerably lower densities of calretinin-IR neurons were observed in the other layers. Parvalbumin-IR neurons were predominantly located in layers III, IV, V, and VI. In layers I and II, parvalbumin-IR neurons were only rarely seen. The majority of the calbindin D28K-IR neurons were stellate, round or oval cells with multipolar dendrites. The majority of calretinin-IR neurons were vertical fusiform cells with long processes traveling perpendicularly to the pial surface. The morphology of the majority of parvalbumin-IR neurons was similar to that of calbindin D28K: stellate, round or oval with multipolar dendrites. These results indicate that these three different calcium-binding proteins are contained in specific layers and cells in the rabbit visual cortex.
Park
H J
HJ
Department of Biology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
Lee
S N
SN
Lim
H R
HR
Kong
J H
JH
Jeon
C J
CJ
D000818:Animals; D064032:Calbindin 2; D064026:Calbindins; D048429:Cell Size; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D010320:Parvalbumins / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D064030:S100 Calcium Binding Protein G / Q000032:analysis* / Q000276:immunology; D014793:Visual Cortex / Q000737:chemistry* / Q000166:cytology
D064032:Calbindin 2; D064026:Calbindins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G