title: A proteomic analysis of secreted proteins from xylan-induced Bacillus sp. strain K-1.
abstract: The expression level of extracellular proteins in an alkaliphilic bacterium, Bacillus sp. strain K-1, grown in a xylan-containing medium, is significantly increased when compared with that grown in the nonxylan culture medium. A proteomic approach has been efficiently applied to separate and characterize these differentially expressed secretory proteins. Eight prominent protein spots were identified and subjected to N-terminal amino acid sequencing. The results show that three spots share considerable similarity with the xylanolytic enzymes and that two spots share considerable similarity with the GltC regulatory protein and 3-dehydroquinate dehydratase, respectively. In addition, the three other proteins show little similarity with the known proteins in the database. In conclusion, our results demonstrate that the proteomic approach is a highly efficient method to rapidly study the differential expression of the secreted proteins by Bacillus sp. strain K-1 grown under xylan-induced condition.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
authors.forename: P W
authors.initials: PW
authors.lastname: Chu
authors.forename: M N
authors.initials: MN
authors.lastname: Yap
authors.forename: C Y
authors.initials: CY
authors.lastname: Wu
authors.forename: C M
authors.initials: CM
authors.lastname: Huang
authors.forename: F M
authors.initials: FM
authors.lastname: Pan
authors.forename: M J
authors.initials: MJ
authors.lastname: Tseng
authors.forename: S T
authors.initials: ST
authors.lastname: Chen
mesh_terms: D001407:Bacillus / Q000187:drug effects* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020543:Proteome / Q000096:biosynthesis*; D013997:Time Factors; D014990:Xylans / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome; D014990:Xylans