Evaluation of indirect fluorescent antibody test and enzyme-linked immunosorbent assay for diagnosis of hepatic amebiasis in Bangladesh.
Serum samples of 31 amebic liver abscess (ALA) patients, 8 amebic hepatitis (AH) patients, and 60 controls were tested for anti-amebic IgG by enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody tests (IFAT). Sera of 29 (93.6%) ALA and 6 (75%) AH patients and 2 (3.3%) control subjects were positive by IFAT. Anti-amebic antibody titer above the cutoff point (= 0.168; x + 2 SD of control sera) was observed in sera of 27 (87%) ALA, 4 (50%) AH, and 1 (1.7%) control by ELISA. All the 8 pus samples were positive for anti-amebic antibodies by IFAT and ELISA. Sensitivity of ELISA was 87% for ALA, with a positive predictive value of 0.96, and 50% for AH cases, with a positive predictive value of 0.80. The sensitivity of IFAT was 93.6% for ALA, with a positive predictive value of 0.94, and 75% for AH, with a positive predictive value of 0.75. When pus samples were tested, the sensitivity was 100% for both tests. The specificity was 98.3% for ELISA and 96.7% for IFAT. Although not significant, IFAT was found more sensitive than ELISA (P>0.05).
Shamsuzzaman
S M
SM
Department of Parasitology, Kochi Medical School, Japan.
Haque
R
R
Hasin
S K
SK
Hashiguchi
Y
Y
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D001459:Bangladesh; D016022:Case-Control Studies; D016001:Confidence Intervals; D004748:Entamoeba histolytica / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D008101:Liver Abscess, Amebic / Q000175:diagnosis* / Q000276:immunology; D011237:Predictive Value of Tests; D012016:Reference Values; D012680:Sensitivity and Specificity; D013492:Suppuration / Q000276:immunology
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G