Mechanical stress regulation and proliferation of rat mandibular condylar chondrocytes in vitro.
OBJECTIVE
To study the proliferative changes of cultured rat mandibular condylar chondrocytes effected by mechanical stress.


METHODS
Flow cytometry (FCM) was used to explore the changes of cellular DNA content and cell cycles of passaged condylar chondrocytes.


RESULTS
FCM results indicated that proliferation of cultured rat mandibular condylar chondrocytes decreased with stress duration, with marked differences between the experimental and control groups.


CONCLUSIONS
Within given force duration and force range (0-207 g/an2), proliferation of rat mandibular condylar chondrocytes increased with force magnitude, reaching the maximum 6 hours after application of stress. Cellular proliferation decreased when the chondrocytes were cultured under continuous stress for 24 hours. The mechanism for these proliferation changes requires further study.
Yang
H
H
Department of Orthodontics, College of Stomatology, West China University of Medical Sciences, Chengdu, Sichuan, P. R. China.
Luo
S
S
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D003799:Dental Stress Analysis; D005434:Flow Cytometry; D008335:Mandibular Condyle / Q000737:chemistry*; D051381:Rats; D013314:Stress, Mechanical