title: Valproic acid intoxication identified by 1H and 1H-(13)C correlated NMR spectroscopy of urine samples.
abstract: Analysis of biological fluids by proton and carbon nuclear magnetic resonance spectroscopy (1H and 13C NMR) is a promising tool in clinical biology. We used this method for rapid toxicological screening in the case of two suicide attempts. For each case, a urine sample was analysed at 300 MHz by 1D and 2D sequences (TOCSY and HMBC) in a short experimental time. Quantification was performed by peak integration on the 1D 1H NMR spectrum. For the two patients, results showed the same resonances of the major metabolite, valproyl-O-glucuronide at concentrations of 121 and 44 mmol/l.
authors.affiliation: Laboratoire de Physique, UPRESA CNRS 8009, Laboratoire d'Application RMN de l'Universit¿ de Lille 2, BP 83, 59006 Cedex, Lille, France.
authors.forename: N
authors.initials: N
authors.lastname: Azaroual
authors.forename: M
authors.initials: M
authors.lastname: Imbenotte
authors.forename: B
authors.initials: B
authors.lastname: Cartigny
authors.forename: F
authors.initials: F
authors.lastname: Leclerc
authors.forename: L
authors.initials: L
authors.lastname: Vallée
authors.forename: M
authors.initials: M
authors.lastname: Lhermitte
authors.forename: G
authors.initials: G
authors.lastname: Vermeersch
mesh_terms: D000293:Adolescent; D000328:Adult; D000927:Anticonvulsants / Q000378:metabolism / Q000506:poisoning* / Q000652:urine*; D002247:Carbon Isotopes; D062787:Drug Overdose; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D013406:Suicide, Attempted; D014635:Valproic Acid / Q000031:analogs & derivatives / Q000378:metabolism / Q000506:poisoning* / Q000652:urine*
chemical_list: D000927:Anticonvulsants; D002247:Carbon Isotopes; C041285:valproic acid glucuronide; D014635:Valproic Acid; D006859:Hydrogen