title: Intrascleral concentration vs depth profile of mitomycin-C after episcleral application: impact of applied concentration and volume of mitomycin-C solution.
abstract: The purpose of this study was to investigate the impact of different concentrations and volumes of Mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. The episcleral sides of scleral quadrants of human donor eyes were exposed for 1 min to sponges (corneal light shield, Merocel Corp.) soaked with MMC. After irrigation with 40 ml saline a central 8 mm diameter scleral disk was horizontally dissected with a cryotome. MMC concentrations of six layers of 140 microns thickness were analysed by means of high-performance liquid chromatography. In Experiment 1 (11 eyes) the sponges were soaked with 50 microliters of 10, 100 and 200 micrograms ml-1 MMC solutions. In Experiment 2 (12 eyes) the sponges were soaked with 10, 30, 50 and 80 microliters of a 200 micrograms ml-1 isotonic MMC solution. In Experiment 1 the MMC concentrations (microgram g-1) of layer 1 were 0.35 (+/- 0.20; 10 micrograms ml-1 group) and 9.22 (+/- 2.92; 200 micrograms ml-1 group). In Experiment 2 the MMC concentrations were 2.57 (+/- 1.17; 10 microliters group), 7.35 (+/- 2.49; 30 microliters group) and 11.67 (+/- 3.25; 80 microliters group). The scleral MMC concentrations were significantly influenced by the applied concentrations (layers 1-5) and by the applied volumes (all layers) of MMC solution. The intrascleral MMC concentration increased linearly with increasing concentration and not linearly with increasing volume of the applied MMC solution. To achieve more predictable scleral concentrations of MMC after trabeculectomy with MMC it seems advisable to control both the concentration and the volume of the MMC solution used to soak the sponge.
authors.affiliation: Department of Ophthalmology, University of Vienna, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Vass
authors.forename: M
authors.initials: M
authors.lastname: Georgopoulos
authors.forename: I
authors.initials: I
authors.lastname: el Menyawi
authors.forename: S
authors.initials: S
authors.lastname: Radda
authors.forename: P
authors.initials: P
authors.lastname: Nimmerrichter
mesh_terms: D000042:Absorption; D000287:Administration, Topical; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005901:Glaucoma / Q000378:metabolism / Q000601:surgery; D006801:Humans; D016685:Mitomycin / Q000493:pharmacokinetics*; D019384:Nucleic Acid Synthesis Inhibitors / Q000493:pharmacokinetics*; D012590:Sclera / Q000187:drug effects / Q000378:metabolism*; D014130:Trabeculectomy
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin