Heterocomplex formation between metastasis-related protein S100A4 (Mts1) and S100A1 as revealed by the yeast two-hybrid system.
S100A4 (Mts1) is a Ca(2+)-binding protein of the S100 family. This protein plays an important role in promoting tumor metastasis. In order to identify S100A4 interacting proteins, we have applied the yeast two-hybrid system as an in vivo approach. By screening a mouse mammary adenocarcinoma library, we have demonstrated that S100A4 forms a heterocomplex with S100A1, another member of the S100 family. The non-covalent heterodimerization was confirmed by fluorescence spectroscopy and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Mutational analysis revealed that replacement of Cys(76) and/or Cys(81) of S100A4 by Ser abolishes the S100A4/S100A1 heterodimerization, but does not affect the S100A4 homodimerization in vivo.
Tarabykina
S
S
Department of Molecular Cancer Biology, Danish Cancer Society, Strandboulevarden 49, Copenhagen 2100, Denmark. svetlana@mcb.molbio.ku.dk
Kriajevska
M
M
Scott
D J
DJ
Hill
T J
TJ
Lafitte
D
D
Derrick
P J
PJ
Dodson
G G
GG
Lukanidin
E
E
Bronstein
I
I
D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D051379:Mice; D009362:Neoplasm Metastasis; D011485:Protein Binding; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence
D002135:Calcium-Binding Proteins; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C110294:S100A1 protein; C470944:S100a4 protein, mouse