Increased urinary excretion of endothelin during hypothermic perfusion preservation in kidneys subjected to preretrieval warm ischemic injury.
The purpose of the study was two-fold: 1) to determine whether endothelin (ET) levels could be detected in the ureteral effluent during hypothermic perfusion preservation (HPP) and; 2) to determine whether preretrieval warm ischemic (WI) injury is associated with increased ureteral excretion of ET. In situ pre-WI injury was induced in Lewis rats (n=10) by a 30-min extrinsic occlusion of the suprarenal aorta. The left kidney underwent 16 hr of HPP, and ureteral effluent (UE) from ischemic and control kidneys (n=10) was collected over 16 hr of HPP. The UE ET concentration and total ET excretion over 16 hr of HPP were significantly higher in kidneys subjected to pre-WI injury compared with nonischemic controls. Kidneys subjected to pre-WI injury can be distinguished from nonischemic control kidneys during HPP by a significantly higher concentration of ET in the UE and a higher overall excretion of ET during HPP.
Inman
S R
SR
Department of Urology, Loyola University of Chicago Stritch School of Medicine and the Hines Veterans Administration Hospital, Maywood, Illinois 60153, USA.
Burns
T E
TE
Osgood
R W
RW
Plott
W K
WK
Lewis
R M
RM
D000818:Animals; D016232:Endothelins / Q000652:urine*; D007511:Ischemia; D007668:Kidney / Q000098:blood supply / Q000502:physiology; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D013696:Temperature; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014513:Ureter / Q000502:physiology
D016232:Endothelins