Characterisation of the carbohydrate components of Taenia solium metacestode glycoprotein antigens.
Human neurocysticercosis is caused by Taenia solium metacestodes. It usually affects the central nervous system of humans and can be confused with other brain pathologies. The Lens culinaris-binding glycoproteins from this parasite have been shown to be ideal targets for the development of a highly specific immunoassay for the diagnosis of neurocysticercosis. In the present study we characterised the carbohydrates associated with five antigenic glycoproteins of T. solium metacestodes in the range of 12-28 kilodaltons. Lectin-affinities and enzymatic deglycosylations suggested that each of the five antigens contain various glycoforms of asparagine-linked carbohydrates of the hybrid, complex and probably high mannose type. These carbohydrates accounted for at least 30-66% of the apparent molecular mass of the glycoconjugates. In contrast, there was no evidence for the presence of O-linked carbohydrates. Lectin affinity patterns suggested that the sugars are short and truncated in their biosynthetic route, and that some contain terminal galactose moieties. Elucidating the precise structure of the carbohydrates and establishing their role in antigenicity will be essential to design strategies to produce them in large and reproducible amounts for the development of improved immunoassays.
Restrepo
B I
BI
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia. blancos@epm.net.co
Obregón-Henao
A
A
Mesa
M
M
Gil
D L
DL
Ortiz
B L
BL
Mejía
J S
JS
Villota
G E
GE
Sanzón
F
F
Teale
J M
JM
D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry*; D001665:Binding Sites; D015153:Blotting, Western; D002241:Carbohydrates / Q000737:chemistry*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D013621:Taenia / Q000737:chemistry*
D000947:Antigens, Helminth; D002241:Carbohydrates