title: Chemiluminescence immunometric assay for measuring osteocalcin in healthy children.
abstract: Osteocalcin (OC) was measured in serum samples from 92 children and adolescents (57 females, 35 males) by a two-site chemiluminescence immunometric assay (Nichols Institute Diagnostics, USA), which recognizes the 1-19 region of the whole molecule as well as the large N-terminal midregion fragment representing the main part of OC in serum. The highest OC levels are measured between the age of 10-15 years. The linear correlations between OC and alkaline phosphatase were 0.65 (p < .01) for total alkaline phosphatase (TAP) and 0.61 (p < 0.1) for bone alkaline phosphatase (BAP).
authors.affiliation: Children's Hospital, University of Cologne.
authors.forename: O
authors.initials: O
authors.lastname: Fricke
authors.forename: A
authors.initials: A
authors.lastname: Stabrey
authors.forename: E
authors.initials: E
authors.lastname: Schönau
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000378:metabolism; D001842:Bone and Bones / Q000201:enzymology; D002648:Child; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006801:Humans; D007118:Immunoassay; D008163:Luminescent Measurements; D008297:Male; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000097:blood*; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011933:Reagent Kits, Diagnostic
chemical_list: D000939:Epitopes; D010446:Peptide Fragments; D011933:Reagent Kits, Diagnostic; D015675:Osteocalcin; D000469:Alkaline Phosphatase