A novel gene derived from developing spinal cords, SCDGF, is a unique member of the PDGF/VEGF family.
We isolated a novel gene designated spinal cord-derived growth factor (SCDGF). Its expression was increased in chick spinal cords with embryonic development and decreased after hatching. The amino acid sequences of chick and human SCDGFs revealed a putative signal sequence followed by a CUB domain and a region homologous to the members of the platelet-derived growth factor/vascular endothelial growth factor family. Furthermore, human SCDGF secreted from the cells showed a mitogenic activity for 10T1/2 cells in vitro. These results led us to speculate that SCDGF plays an important role in the development of the spinal cord.
Hamada
T
T
Department of Pharmacology, Nippon Medical School, Tokyo, Japan.
Ui-Tei
K
K
Miyata
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D002642:Chick Embryo; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000378:metabolism; D016228:Endothelial Growth Factors / Q000235:genetics; D006801:Humans; D008222:Lymphokines / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D005810:Multigene Family; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013116:Spinal Cord / Q000196:embryology / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D018076:DNA, Complementary; D016228:Endothelial Growth Factors; D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C411423:platelet-derived growth factor C