[Antiretinal antibodies associated with cystoid macular edema].
OBJECTIVE
Recently, anti-Enolase and anti-carbonic anhydrase antibodies have been observed in over 60% of patients with retinitis pigmentosa (RP) and cystoid macular oedema (CME). We investigated the presence of these antibodies in a series of patients with CME due to different pathologies.


METHODS
In 10 patients with CME serum antibodies against Carbonic anhydrase (CA) II (30 kD) and Enolase (46 kD) were sought using Western Blots, Dot Blots as well as ELISA.


RESULTS
Western and dot blotting showed anti-CA II antibodies in all and anti-Enolase antibodies in six of the 10 patients. The average titer measured with ELISA was 0.9 +/- 0.08 OD Units (0.35-1.4) with a dilution of 1:400.


CONCLUSIONS
The presence of anti-retinal antibodies in the serum of all patients confirms the high prevalence of these antibodies in patients with CME. This may suggest that a dysfunction of CA and enolase activity in the retinal pigment epithelium may lie at the root of oedema formation, whereas other mechanisms may be responsible in the absence of these antibodies.
Wolfensberger
T J
TJ
Hôpital Ophtalmique Jules Gonin, Université de Lausanne, Suisse. tjw@pingnet.ch
Aptsiauri
N
N
Godley
B
B
Downes
S
S
Bird
A C
AC
D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D015153:Blotting, Western; D002256:Carbonic Anhydrases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008269:Macular Edema / Q000209:etiology / Q000276:immunology*; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000276:immunology*; D012160:Retina / Q000201:enzymology / Q000276:immunology*
D001323:Autoantibodies; D002256:Carbonic Anhydrases; D010751:Phosphopyruvate Hydratase
Antiretinale Antikörper assoziiert mit zystoidem Makulaödem.