title: Development of chiral HPLC for selenoamino acids with ICP-MS detection:application to selenium nutritional supplements.
abstract: The enantiomeric separation of three underivatized seleno-amino acids, D,L-selenocystine, and D,L-selenomethionine, and D,L-selenomethionine, with UV and ICP-MS detection is described. An HPLC column with a chiral crown ether stationary phase and a mobile phase of 0.10 M HCIO4 was used. Absolute detection limits obtained with UV detection ranged from 34.5 to 47.1 ng whereas those obtained with the plasma detector were ca. 40-400 times better. The separations with either detector were good, with the little detector effect on the resolution. Ten commercially available dietary selenium supplements were analyzed using the chiral column to identify and quantify the selenium species present with both detection modes. Selenium species were easily identified using ICP-MS detection, whereas UV detection was not viable because of interferences from the sample matrix and inadequate sensitivity. Selenium species that were unretained using the chiral column were identified using anion exchange chromatography. Total amounts in the samples were also measured using a conventional digestion and enzymatic digestion with ICP-MS detection.
authors.affiliation: Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Sutton
authors.forename: C A
authors.initials: CA
authors.lastname: Ponce de Leon
authors.forename: K L
authors.initials: KL
authors.lastname: Ackley
authors.forename: R M
authors.initials: RM
authors.lastname: Sutton
authors.forename: A M
authors.initials: AM
authors.lastname: Stalcup
authors.forename: J A
authors.initials: JA
authors.lastname: Caruso
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements; D013058:Mass Spectrometry / Q000379:methods*; D012643:Selenium / Q000032:analysis*; D018036:Selenium Compounds / Q000737:chemistry*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
chemical_list: D018036:Selenium Compounds; D012643:Selenium