Solid-state fermentation with Aspergillus niger for cellobiase production.
Aspergillus niger NRRL3 was cultivated in a moist wheat bran and ground corncob solid medium supplemented with inorganic minerals for the production of cellobiase (beta-1,4-glucosidase, EC 3.2.1.21). With this method, A. niger NRRL3 was able to produce a high concentration of cellobiase (215 IU/g of solid substrate) after 96 h of incubation. Temperature and moisture content affected final cellobiase titers. The best conditions for cellobiase production from solid substrate by A. niger NRRL3 were determined to be 70% moisture and 35 degrees C.
Tsao
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Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907, USA. tsaogt@ecn.purdue.edu
Xia
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Cao
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Gong
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D001230:Aspergillus / Q000201:enzymology*; D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development*; D001431:Bacteriological Techniques; D001709:Biotechnology; D005285:Fermentation; D007700:Kinetics; D013045:Species Specificity; D013696:Temperature; D001617:beta-Glucosidase / Q000096:biosynthesis*
D001617:beta-Glucosidase