title: Cloning of the gene coding for Staphylococcus hyicus exfoliative toxin B and its expression in Escherichia coli.
abstract: The Staphylococcus hyicus exfoliative toxin B (SHETB) gene was cloned into pUC118 and expressed in Escherichia coli. The nucleotide sequence of the SHETB gene consists of a coding region of 804 bp specifying a polypeptide of 268 amino acid residues, which included a putative 20-residue signal sequence.
authors.affiliation: Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Hatakeyama
authors.forename: T
authors.initials: T
authors.lastname: Matsuzawa
authors.forename: M
authors.initials: M
authors.lastname: Kawai
authors.forename: C
authors.initials: C
authors.lastname: Aizawa
authors.forename: H
authors.initials: H
authors.lastname: Danbara
authors.forename: N
authors.initials: N
authors.lastname: Maehara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005083:Exfoliatins / Q000096:biosynthesis / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D013210:Staphylococcus / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D005083:Exfoliatins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins