Characterization of immune response to synthetic peptides derived from the hemagglutinin of measles virus.
We describe here four synthetic peptides derived from the hemagglutinin of measles virus. The peptides were predicted by a computer program combining hydrophilicity, flexibility, surface probability, secondary structure and antigenic index parameters of the amino acid sequence of measles virus hemagglutinin. Rabbits were immunized with the synthesized peptides conjugated to purified protein derivative using immunostimulating complex as adjuvant. Anti-peptide antisera raised in rabbits against the peptide conjugates reacted well with the homologous peptides and with measles virus antigen as tested with plate ELISA. None of these sera had either neutralizing or hemagglutination inhibiting antibody or reacted with measles hemagglutinin protein in Western blot and reacted weakly in immunofluorescence. Human sera positive for measles virus antibody reacted with the synthesized peptides indicating that the selected locations function as partial antigenic sites.
Meqdam
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Department of Applied Biology, Jordan University of Science and Technology, Irbid. meqdam@just.edu.jo
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D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D006389:Hemagglutinins, Viral / Q000276:immunology*; D006801:Humans; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D009842:Oligopeptides; D010446:Peptide Fragments; C032397:hemagglutinin protein G, measles virus