False-positive results for Mycobacterium celatum with the AccuProbe Mycobacterium tuberculosis complex assay.
Mycobacterium celatum type 1 was found to cross-react in the AccuProbe Mycobacterium tuberculosis complex assay. Subsequently, we found a statistically significant increase in the relative light units with lower temperatures, suggesting that it is necessary to perform this AccuProbe assay at between 60 and 61 degrees C. We also recommend the inclusion of M. celatum type 1 as a negative control.
Somoskövi
A
A
Wadsworth Center, New York State Department of Health, Albany, New York 12201-0509, USA.
Hotaling
J E
JE
Fitzgerald
M
M
Jonas
V
V
Stasik
D
D
Parsons
L M
LM
Salfinger
M
M
D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005189:False Positive Reactions; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D011933:Reagent Kits, Diagnostic; D013696:Temperature; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000382:microbiology*
D015342:DNA Probes; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic