Induction of reparative dentin formation in dogs with combined recombinant human bone morphogenetic protein 2 and fibrin sealant.
OBJECTIVE
To investigate the reparative dentin formation induced by the complex of recombinant human bone morphogenetic protein 2 (rhBMP2) and fibrin sealant (FS) in dogs.


METHODS
Freshly exposed pulp of molars, premolars, and canines were treated, respectively, with the complex of rhBMP2-FS, rhBMP2, and Ca(OH)2 paste, which served as control.


RESULTS
Wound healing of exposed pulp treated with the complex of rhBMP2 and FS seemed better than that with rhBMP2 alone. Dentin bridge formation was observed at 1 week when pulp was treated with the complex and at 4 weeks with rhBMP2 and Ca(OH)2. At 9 weeks after operation, more amount of tubular dentin bridge formation was found in pulp treated with the complex than with rhBMP2.


CONCLUSIONS
The results suggest that synergistic effects of fibrin and rhBMP2 existed and that rh-BMP2 with FS carrier can be used as bioactive pulp capping agent. Together, these agents can induce a large amount of dentin and enhance healing of exposed pulp.
Ren
W H
WH
Department of Oral Pathology, College and Hospital of Stomatology, Fourth Military Medical University (FMMU), Xian, P. R. China.
Yang
L J
LJ
Dong
S Z
SZ
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D003782:Dental Pulp / Q000187:drug effects*; D003809:Dentin, Secondary / Q000187:drug effects* / Q000254:growth & development; D003810:Dentinogenesis / Q000187:drug effects; D004285:Dogs; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D015718:Fibrin Tissue Adhesive; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2