[Dried nutrient media for the cultivation of pneumococcus and meningococcus].
Dried synthetic nutrient medium for the cultivation of meningococci and the accumulation of their biomass has been developed. The kinetics of the culture growth, changes in pH and in the content of dissolved oxygen in the medium during prolonged controlled processes of the cultivation of meningococci in a bioreactor with the use of this medium have been studied. The stable physico-chemical properties and composition of the polysaccharide-protein complex isolated from the biomass of meningococci grown in the above-mentioned medium have made it possible to use it for the preparation of the samples of group B meningococcal vaccine. In addition, dried semi-synthetic nutrient medium for the accumulation of pneumococci without the necessity of introducing blood or serum into the medium has been developed. As regards its biological properties, this newly developed medium make it not inferior to meat media, containing blood or serum, and ensures good yield of biomass.
Omarova
S M
SM
Research and Production Amalgamation Nutrient Media, Makhachkala, Russia.
Basnak'ian
I A
IA
Artem'eva
T A
TA
D001431:Bacteriological Techniques; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003470:Culture Media / Q000737:chemistry / Q000592:standards; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009345:Neisseria meningitidis / Q000254:growth & development* / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000254:growth & development* / Q000302:isolation & purification
D003470:Culture Media
Sukhie pitatel'nye sredy dlia kul'tivirovaniia pnevmokokkov i meningokokkov.