Caffeine induces in vivo premature appearance of telencephalic vesicles.
Caffeine administered to pregnant mice during germinative neuroepithelium preparation (embryonic days 8-10) dramatically accelerated primitive neuroepithelium evagination into telencephalic vesicles, versus age-matched controls. This histologically-documented, dose-dependent effect seemed reversible during subsequent neuronal migration if caffeine exposure was discontinued. Our in vivo model provides a new tool for studying telencephalic symmetry acquisition and for identifying genes potentially involved in holoprosencephaly, a developmental disorder characterized by defective telencephalic vesicle formation.
Sahir
N
N
Service de Neurologie Pédiatrique, Hôpital Robert-Debré, 48 Blvd. Sérurier, 75019, Paris, France.
Bahi
N
N
Evrard
P
P
Gressens
P
P
D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D016142:Holoprosencephaly / Q000139:chemically induced*; D051379:Mice; D011247:Pregnancy; D013687:Telencephalon / Q000187:drug effects* / Q000196:embryology*
D000697:Central Nervous System Stimulants; D002110:Caffeine