title: Mycobacterium smegmatis has two pyrazinamidase enzymes, PncA and pzaA.
abstract: The Mycobacterium smegmatis pncA gene, encoding nicotinamidase/pyrazinamidase, was identified. While it was similar to counterparts from other mycobacteria, the M. smegmatis PncA had little homology to the other M. smegmatis pyrazinamidase/nicotinamidase, encoded by the pzaA gene. Transformation of Mycobacterium bovis strain BCG with M. smegmatis pncA or pzaA conferred susceptibility to pyrazinamide.
authors.affiliation: Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
authors.forename: M
authors.initials: M
authors.lastname: Guo
authors.forename: Z
authors.initials: Z
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000235:genetics; D009535:Nicotinamidase / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D000581:Amidohydrolases; C022490:pyrazinamide deamidase; D009535:Nicotinamidase