title: Interaction between the transcription factor SPBP and the positive cofactor RNF4. An interplay between protein binding zinc fingers.
abstract: The activator of stromelysin 1 gene transcription, SPBP, interacts with the RING finger protein RNF4. Both proteins are ubiquitously expressed and localized in the nucleus. RNF4 facilitates accumulation of specific SPBP-DNA complexes in vitro and acts as a positive cofactor in SPBP-mediated transactivation. SPBP harbors an internal zinc finger of the PHD/LAP type. This domain can form intra-chain protein-protein contacts in SPBP resulting in negative modulation of SPBP-RNF4 interaction.
authors.affiliation: Department of Molecular and Structural Biology, University of Aarhus, C. F. Mollers Alle 130, DK-8000 Aarhus C., Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Lyngsø
authors.forename: G
authors.initials: G
authors.lastname: Bouteiller
authors.forename: C K
authors.initials: CK
authors.lastname: Damgaard
authors.forename: D
authors.initials: D
authors.lastname: Ryom
authors.forename: S
authors.initials: S
authors.lastname: Sanchez-Muñoz
authors.forename: P L
authors.initials: PL
authors.lastname: Nørby
authors.forename: B J
authors.initials: BJ
authors.lastname: Bonven
authors.forename: P
authors.initials: P
authors.lastname: Jørgensen
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010449:Peptide Mapping; D011485:Protein Binding; D012441:Saccharomyces cerevisiae; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C114070:RNF4 protein, human; C114750:TCF20 protein, human; D014157:Transcription Factors; C486410:Rnf4 protein, mouse; D044767:Ubiquitin-Protein Ligases; D019278:Matrix Metalloproteinase 3