Chemiluminescence and reactive oxygen scavenging activities of the hydrogen peroxide/gallic acid/lactoperoxidase system.
Photon emission in the hydrogen peroxide/gallic acid/lactoperoxidase system was studied using visible region chemiluminescence. The photon emission intensity showed a pH-dependence curve with a maximum at pH 4.5-5.0. The spectral analysis showed to be at 510 nm and its energy was 61.0 kcal/mol at either pH 4.5 or pH 7.0. In addition, near-infrared spectral analysis at 1270 nm suggested that this photon emission from the hydrogen peroxide/gallic acid/lactoperoxidase system was produced without generation of (1)O(2). The gallic acid/lactoperoxidase system, based on the chemiluminescence system, has superoxide-, hydroxyl radical-scavenging activities and antioxidative activity. These results are strong evidence that the gallic acid/lactoperoxidase system is one of the reactive oxygen scavenging systems.
Yoshiki
Y
Y
Department of Environmental Bioremediation, Graduate School of Agricultural Science, Tohoku University, Japan. yoshiki@bios.tohoku.ac.jp
Okubo
K
K
Akiyama
Y
Y
Sato
K
K
Kawanari
M
M
D000818:Animals; D002417:Cattle; D016166:Free Radical Scavengers / Q000378:metabolism*; D005707:Gallic Acid / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007784:Lactoperoxidase / Q000378:metabolism*; D019787:Linoleic Acid / Q000378:metabolism; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism; D013816:Thermodynamics
D016166:Free Radical Scavengers; D013481:Superoxides; D005707:Gallic Acid; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D007784:Lactoperoxidase