title: Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis.
abstract: We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation.
authors.affiliation: Molecular Biology and Virology Laboratory, The Salk Institute, La Jolla, CA 92037, USA.
authors.forename: D T
authors.initials: DT
authors.lastname: Bishop
authors.forename: W H
authors.initials: WH
authors.lastname: McDonald
authors.forename: K L
authors.initials: KL
authors.lastname: Gould
authors.forename: S L
authors.initials: SL
authors.lastname: Forsburg
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012322:RNA Precursors / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics*; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D016196:S Phase / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment
chemical_list: D005656:Fungal Proteins; C065573:PRP6 protein, S cerevisiae; D012322:RNA Precursors; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D017415:Ribonucleoprotein, U5 Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C491450:prp31 protein, S pombe