A shaker-like K(+) channel with weak rectification is expressed in both source and sink phloem tissues of Arabidopsis.
RNA gel blot and reverse transcription-polymerase chain reaction experiments were used to identify a single K(+) channel gene in Arabidopsis as expressed throughout the plant. Use of the beta-glucuronidase reporter gene revealed expression of this gene, AKT2/AKT3, in both source and sink phloem tissues. The AKT2/AKT3 gene corresponds to two previously identified cDNAs, AKT2 (reconstructed at its 5' end) and AKT3, the open reading frame of the latter being shorter at its 5' end than that of the former. Rapid amplification of cDNA ends with polymerase chain reaction and site-directed mutagenesis was performed to identify the initiation codon for AKT2 translation. All of the data are consistent with the hypothesis that the encoded polypeptide corresponds to the longest open reading frame previously identified (AKT2). Electrophysiological characterization (macroscopic and single-channel currents) of AKT2 in both Xenopus oocytes and COS cells revealed a unique gating mode and sensitivity to pH (weak inward rectification, inhibition, and increased rectification upon internal or external acidification), suggesting that AKT2 has enough functional plasticity to perform different functions in phloem tissue of source and sink organs. The plant stress hormone abscisic acid was shown to increase the amount of AKT2 transcript, suggesting a role for the AKT2 in the plant response to drought.
Lacombe
B
B
Biochimie et Physiologie Moléculaire des Plantes, UMR 5004, Agro-M/CNRS/INRA/UM2, Place Viala, 34060 Montpellier Cedex 1, France.
Pilot
G
G
Michard
E
E
Gaymard
F
F
Sentenac
H
H
Thibaud
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029681:Arabidopsis Proteins; D019556:COS Cells; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009865:Oocytes; D018408:Patch-Clamp Techniques; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D018514:Plant Structures / Q000378:metabolism; D030821:Plants, Genetically Modified; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014981:Xenopus
C097485:AKT2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D010940:Plant Proteins; D015221:Potassium Channels