title: The effect of elastomeric impression materials on the growth of micro-organisms.
abstract: The influence of elastomeric impression materials on the growth of micro-organisms was examined in vitro. Bacillus subtilis was inoculated into broth containing impression materials and incubated at 37 degrees C for 72 hours. Express STD Putty, President Putty and Jet-Light Body, Low and Very High Viscosity Permagum and Provil L stimulated growth whereas Impregum-F and Express Light Body inhibited growth. The influence of Impregum-F and Express Light Body on oral micro-organisms was investigated further. Broth extracts were prepared by soaking these materials in Todd Hewitt broth for either 5 or 10 minutes. Thereafter, the extracts were inoculated with oral strains of Staphylococcus aureus, Streptococcus mutans and Candida albicans, incubated at 37 degrees C for 72 hours and plated on blood and Sabourauds agar to test for the presence of viable micro-organisms. The 10-minute broth extracts killed all the test isolates which suggests that impressions taken with Impregum-F and Express Light Body may not require disinfecting.
authors.affiliation: Department of Restorative Dentistry, University of the Witwatersrand, Johannesburg.
authors.forename: C M
authors.initials: CM
authors.lastname: Tuit
authors.forename: M M
authors.initials: MM
authors.lastname: Coogan
mesh_terms: D000704:Analysis of Variance; D002176:Candida albicans / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003748:Dental Equipment / Q000382:microbiology*; D003760:Dental Impression Materials; D004866:Equipment Contamination; D008826:Microbial Sensitivity Tests; D011145:Polyvinyls; D012826:Silicone Elastomers; D012833:Siloxanes; D013211:Staphylococcus aureus / Q000302:isolation & purification; D013295:Streptococcus mutans / Q000302:isolation & purification
chemical_list: D003760:Dental Impression Materials; D011145:Polyvinyls; D012826:Silicone Elastomers; D012833:Siloxanes; C034183:vinyl polysiloxane