Synthesis and hybridization properties of polyamide based nucleic acid analogues incorporating pyrrolidine-derived nucleoamino acids.
Ndelta-Fmoc protected nucleoamino acids of type I (Base = T, C, A) have been synthesized and employed as building blocks for the construction of novel polyamide based nucleic acid analogues. Homopyrimidine oligomer A binds to complementary RNA with significant affinity and in a sequence-specific fashion, while no binding was observed to complementary DNA.
Altmann
K H
KH
Novartis Pharma AG, TA Oncology Research, Basel, Switzerland. karl-heinz.altmann@pharma.novartis.com
Hüsken
D
D
Cuenoud
B
B
García-Echeverría
C
C
D000596:Amino Acids / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D018076:DNA, Complementary / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009696:Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D009757:Nylons / Q000138:chemical synthesis* / Q000737:chemistry; D011759:Pyrrolidines / Q000737:chemistry*; D018075:RNA, Complementary / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D018076:DNA, Complementary; D009696:Nucleic Acids; D009757:Nylons; D011759:Pyrrolidines; D018075:RNA, Complementary