The expression frequency of common fragile sites and genetic predisposition to colon cancer.
The expression frequency of common fragile sites induced by aphidicolin (Apc), bromodeoxyuridine (BrdU), and caffeine was evaluated on prometaphase chromosomes obtained from the peripheral blood lymphocytes of 32 patients with colon cancer, 30 of their clinically healthy family members and 30 age-matched normal controls. The proportion of damaged cells (P < 0.001), the mean number of chromosomal aberrations and the expression frequencies of fragile sites were significantly higher in the patient and relative groups compared to the control group. Our findings show an increased genetic instability in patients with colon cancer and their first-degree relatives. In addition, common fragile sites can be used as a suitable marker for determining genetic predisposition to cancer.
Tunca
B
B
Department of Medical Biology, Medical Faculty of Uludağ University, Bursa, Turkey.
Egeli
U
U
Zorluoglu
A
A
Yilmazlar
T
T
Yerci
O
O
Kizil
A
A
D000230:Adenocarcinoma / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D020087:Alu Elements; D016590:Aphidicolin / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002877:Chromosomes, Human / Q000187:drug effects* / Q000648:ultrastructure; D003110:Colonic Neoplasms / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D020022:Genetic Predisposition to Disease; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D010375:Pedigree
D016590:Aphidicolin; D002110:Caffeine; D001973:Bromodeoxyuridine