title: Identification of protective outer membrane antigens of Brucella ovis by passive immunization of mice with monoclonal antibodies.
abstract: Outer membrane proteins (OMPs) and rough lipopolysaccharide (R-LPS), the main surface antigens of Brucella ovis, display surface-exposed epitopes. Mixtures of monoclonal antibodies (mAbs) to both antigens were previously shown to protect mice against a B. ovis challenge. To further identify the antigens involved, seven mAbs against Brucella OMPs (Omp10, Omp16, Omp19, Omp25, Omp31, Omp2b and Omp1) and three to R-LPS were tested for protection either individually or in combinations. Significant reduction in spleen infection in challenged mice, relative to controls, was used as the protection criteri. Controls included nonimmunized mice and mice given an irrelevant, anti-O-polysaccharide (OPS), mAb. For comparison, a group received a mouse serum containing antibodies to both OMPs and R-LPS; this serum was prepared by immunization with a B. ovis hot-saline extract which, as described previously, induces protective immunity in mice and rams. Significant protection was observed with both mAbs to OMPs and R-LPS. mAbs to Omp16, Omp19 and Omp31 afforded the highest protection and prevented the development of splenomegaly. The protective effect of mAb to Omp31 was not interfered with by nonprotective mAbs in different mixtures. The data presented confirm the protective role of antibodies to OMPs and R-LPS against B. ovis, and identify several OMPs, especially Omp31, which are promising candidates for a subunit vaccine against ram epididymitis.
authors.affiliation: Laboratorio de Inmunoquimica y Biotecnologia, Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, UNICEN, 7000, Tandil, Argentina.
authors.forename: R A
authors.initials: RA
authors.lastname: Bowden
authors.forename: S M
authors.initials: SM
authors.lastname: Estein
authors.forename: M S
authors.initials: MS
authors.lastname: Zygmunt
authors.forename: G
authors.initials: G
authors.lastname: Dubray
authors.forename: A
authors.initials: A
authors.lastname: Cloeckaert
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D015153:Blotting, Western; D002002:Brucella; D002006:Brucellosis / Q000382:microbiology / Q000628:therapy*; D004359:Drug Therapy, Combination; D007116:Immunization, Passive; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides