Simple method for determination of terbutaline plasma concentration by high-performance liquid chromatography.
A method is described in which low nanomolar concentrations of terbutaline in plasma can be quantitated by use of a standard isocratic high-performance liquid chromatography system with electrochemical detection. Samples were prepared for injection by solid-phase extraction and preserved from degradation by addition of glutathione. Terbutaline and internal standard metaproterenol were resolved from plasma constituents on a single C(18) column by ion-pairing chromatography. The method is precise and accurate for measurement of freebase concentrations as low as 4.4 nmol/l (1 ng/ml).
Herring
V L
VL
Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee, Memphis 38163, USA.
Johnson
J A
JA
D001993:Bronchodilator Agents / Q000097:blood* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013726:Terbutaline / Q000097:blood* / Q000493:pharmacokinetics
D001993:Bronchodilator Agents; D013726:Terbutaline