Trypsin/acrosin inhibitor activity of rat and guinea pig caltrin proteins. Structural and functional studies.
Dramatic inhibition of trypsin activity by rat caltrin and guinea pig caltrin I was spectrophotometrically demonstrated using the artificial substrate benzoylarginyl ethyl ester. Approximately 6% and 21% of residual proteolytic activity was recorded after preincubating the enzyme with 0.22 and 0.27 microM rat caltrin and guinea pig caltrin I, respectively. Reduction and carboxymethylation of the cysteine residues abolished the inhibitor activity of both caltrin proteins. Rat caltrin and guinea pig caltrin I show structural homology with secretory trypsin/acrosin inhibitor proteins isolated from boar and human seminal plasma and mouse seminal vesicle secretion and share a fragment of 13 amino acids of almost identical sequence (DPVCGTDGH/K/ITYG/AN), which is also present in the structure of Kazal-type trypsin inhibitor proteins from different mammalian tissues. Bovine, mouse, and guinea pig caltrin II, three caltrin proteins that have no structural homology with rat caltrin or guinea pig caltrin I, lack trypsin inhibitor activity. Rat caltrin, guinea pig caltrin I, and the mouse seminal vesicle trypsin inhibitor protein P12, which also inhibits Ca(2+) uptake into epididymal spermatozoa (mouse caltrin I), bound specifically to the sperm head, on the acrosomal region, as detected by indirect immunofluorescence. They also inhibited the acrosin activity in the gelatin film assay. Caltrin I may play an important role in the control of sperm functions such as Ca(2+) influx in the acrosome reaction and activation of acrosin and other serine-proteases at the proper site and proper time to ensure successful fertilization.
Winnica
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DE
Cátedras de Química Biológica, Facultades de Ciencias Médicas and Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina.
Novella
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ML
Dematteis
A
A
Coronel
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CE
D000176:Acrosin / Q000037:antagonists & inhibitors*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D006023:Glycoproteins; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D029589:Seminal Plasma Proteins; D017386:Sequence Homology, Amino Acid; D013077:Sperm Head / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism; D014359:Trypsin Inhibitor, Kazal Pancreatic; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D006023:Glycoproteins; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C487134:Spink1 protein, mouse; D014361:Trypsin Inhibitors; C043076:beta-microseminoprotein; D014359:Trypsin Inhibitor, Kazal Pancreatic; D000176:Acrosin