[A system for the automated chemotaxonomic determination of salmonellae and other pathogenic bacteria].
On the basis of the data of the gas-chromatographic analysis of the fatty acids of 208 bacterial strains, representatives of 16 different microbial genera, the algorithm of decision taking, necessary for the program provision of investigations, was worked out. In working out the algorithm the characteristics of 30 fatty acids were used, making it possible to classify bacteria with their genera and in some cases their species. The groups of fatty acids with the number of carbon atoms in their molecules ranging from 10 to 25, their melting temperatures and the dependence of relative characteristics of binding from the number of carbon atoms in the molecule of the acid, its chemical composition and the presence of double bonds were taken into consideration. To indicate salmonellae by their fatty acid profiles, a chromatographic system on the basis of a type Crystal 2000 M gas chromatograph is proposed. In addition, the complex method for the determination of bacteria, combining the determination of salmonellae by changes in the medium resistance (impedance) with the use of an electrochemical analyzer and the subsequent identification of the infective agent by its fatty acid profile in the common system of gas-chromatographic investigation, is proposed.
Bondarenko
V M
VM
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Latkin
A T
AT
Komarov
G D
GD
Meshcheriakova
I S
IS
Pomazanov
V V
VV
D000465:Algorithms; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D002849:Chromatography, Gas / Q000379:methods / Q000706:statistics & numerical data; D003470:Culture Media; D004563:Electrochemistry; D005227:Fatty Acids / Q000032:analysis; D012475:Salmonella / Q000737:chemistry* / Q000145:classification* / Q000472:pathogenicity; D013045:Species Specificity; D013997:Time Factors
D003470:Culture Media; D005227:Fatty Acids
Sistema avtomatizirovannogo khemotaksonomicheskogo obnaruzheniia sal'monell i drugikh patogennykh bakteriĭ.