A polymorphism upstream from the human paraoxonase (PON1) gene and its association with PON1 expression.
Human serum paraoxonase (PON1) is an esterase that has been shown to decrease the susceptibility of lipoproteins to lipid peroxidation. We found a polymorphism of cytosine/thymidine (-108C/T, the number is from the ATG codon) in the upstream region of the PON1 gene. The luciferase activity was lower in the -108T allele than in the -108C allele. The serum PON1 concentrations in 132 normal subjects were as follows: -108CC>-108CT and>-108TT genotypes. The polymorphism upstream from the PON1 gene is associated with transcription of the PON1 gene and the serum PON1 concentration.
Suehiro
T
T
The Second Department of Internal Medicine, Kochi Medical School, Kohasu, Okoh-cho, Nankoku, 781-8505, Kochi, Japan. suehirot@kochi-ms.ac.jp
Nakamura
T
T
Inoue
M
M
Shiinoki
T
T
Ikeda
Y
Y
Kumon
Y
Y
Shindo
M
M
Tanaka
H
H
Hashimoto
K
K
D000483:Alleles; D043303:Aryldialkylphosphatase; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003327:Coronary Disease / Q000097:blood / Q000201:enzymology / Q000235:genetics; D004247:DNA / Q000032:analysis*; D017931:DNA Primers / Q000737:chemistry; D004950:Esterases / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D015227:Lipid Peroxidation / Q000235:genetics*; D008156:Luciferases / Q000097:blood; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA; D008156:Luciferases; D004950:Esterases; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human