Dbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis.
Ribosome biogenesis requires, in addition to rRNA molecules and ribosomal proteins, a multitude of trans-acting factors. Recently it has become clear that in the yeast Saccharomyces cerevisiae many RNA helicases of the DEAD-box and related families are involved in ribosome biogenesis. Here we show that the previously uncharacterised open reading frame YDL031w (renamed DBP10 for DEAD-box protein 10) encodes an essential putative RNA helicase that is required for accurate ribosome biogenesis. Genetic depletion of Dbp10p results in a deficit in 60S ribosomal subunits and an accumulation of half-mer polysomes. Furthermore, pulse-chase analyses of pre-rRNA processing reveal a strong delay in the maturation of 27SB pre-rRNA intermediates into 25S rRNA and 7S pre-rRNA. Northern blot analyses indicate that this delay leads to higher steady-state levels of 27SB species and reduced steady-state levels of 7S pre-rRNA and 25S/5.8S mature rRNAs, thus explaining the final deficit in 60S subunit and the formation of half-mer polysomes. Consistent with a direct role in ribosome biogenesis, Dbp10p was found to be located predominantly in the nucleolus.
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Département de Biochimie Médicale, CMU, 1 rue Michel Servet, CH 1211 Genève 4, Switzerland.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002466:Cell Nucleolus / Q000502:physiology; D053487:DEAD-box RNA Helicases; D017353:Gene Deletion; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016366:Open Reading Frames; D010641:Phenotype; D020365:RNA Helicases / Q000235:genetics* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S; D012270:Ribosomes / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins
D009838:Oligodeoxyribonucleotides; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; D029701:Saccharomyces cerevisiae Proteins; C065039:RNA, ribosomal, 25S; C411109:DBP10 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases