Expression of inflammatory cytokines by murine macrophages activated with a new synthetic lipopeptide JT3002.
The present study was undertaken to investigate the effect of JT3002, a new synthetic analogue of a lipoprotein from the outer wall of a gram-negative bacterium on the production of cytokines by mouse peritoneal macrophages. Multilamellar liposomes containing different concentrations of JT3002 induced production of the inflammatory cytokines tumor necrosis factor-alpha, interleukin-1 alpha, and interleukin-6 by macrophages in dose- and time-dependent manners. The presence of interferon-gamma enhanced production of tumor necrosis factor-alpha by macrophages exposed to lower concentrations of JT3002 and induced the release of nitric oxide, a potent cytolytic molecule of activated macrophages. Unlike lipopolysaccharide, JT3002 activated macrophages independently of serum, but like lipopolysaccharide, it required protein tyrosine kinase.
Kumar
R
R
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Eue
I
I
Dong
Z
Z
Killion
J J
JJ
Fidler
I J
IJ
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D055666:Lipopeptides; D008070:Lipopolysaccharides / Q000494:pharmacology; D008074:Lipoproteins / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011505:Protein-Tyrosine Kinases / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; C112524:JBT-3002; D055666:Lipopeptides; D008070:Lipopolysaccharides; D008074:Lipoproteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D011505:Protein-Tyrosine Kinases