A region in the 3' UTR of MnSOD RNA enhances translation of a heterologous RNA.
The studies reported in this paper were designed to test the hypothesis that a cis element located in the 3' UTR of manganese superoxide dismutase (MnSOD) RNA, designated MnSOD-response element (MnSOD-RE), is a translational enhancer in vivo. NIH/3T3 cells were transfected with a posttranscriptional reporter construct in which MnSOD-RE was placed 3' of the coding region of chloramphenicol acetyltransferase (CAT); this construct is designated CAT-RMS. Transient transfection of CAT-RMS did not change the concentration of CAT mRNA but increased CAT activity by approximately 400% compared to a control construct, CAT-V, which contains approximately the same size of non-MnSOD 3' UTR sequence. Transfection of CAT-RMS had no effect on endogenous MnSOD protein, mRNA, or MnSOD RNA-binding protein activity. Because of its ability to increase translation of a heterologous RNA, MnSOD-RE may be useful in designing expression vectors for in vitro expression systems and in vivo gene therapy.
Knirsch
L
L
Department of Pediatrics, Georgetown University School of Medicine, Washington, DC 20007, USA.
Clerch
L B
LB
D020413:3' Untranslated Regions; D016475:3T3 Cells; D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D017930:Genes, Reporter; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000235:genetics*; D014162:Transfection
D020413:3' Untranslated Regions; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D015500:Chloramphenicol O-Acetyltransferase