Interaction between the transcription factor SPBP and the positive cofactor RNF4. An interplay between protein binding zinc fingers.
The activator of stromelysin 1 gene transcription, SPBP, interacts with the RING finger protein RNF4. Both proteins are ubiquitously expressed and localized in the nucleus. RNF4 facilitates accumulation of specific SPBP-DNA complexes in vitro and acts as a positive cofactor in SPBP-mediated transactivation. SPBP harbors an internal zinc finger of the PHD/LAP type. This domain can form intra-chain protein-protein contacts in SPBP resulting in negative modulation of SPBP-RNF4 interaction.
Lyngsø
C
C
Department of Molecular and Structural Biology, University of Aarhus, C. F. Mollers Alle 130, DK-8000 Aarhus C., Denmark.
Bouteiller
G
G
Damgaard
C K
CK
Ryom
D
D
Sanchez-Muñoz
S
S
Nørby
P L
PL
Bonven
B J
BJ
Jørgensen
P
P
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004926:Escherichia coli; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010449:Peptide Mapping; D011485:Protein Binding; D012441:Saccharomyces cerevisiae; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D044767:Ubiquitin-Protein Ligases; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C114070:RNF4 protein, human; C114750:TCF20 protein, human; D014157:Transcription Factors; C486410:Rnf4 protein, mouse; D044767:Ubiquitin-Protein Ligases; D019278:Matrix Metalloproteinase 3