Glycosaminoglycans alter the conformation of interferon-gamma.
Interferon-gamma (IFN-gamma) is a potent immunomodulatory cytokine whose physiological roles are modulated by the extracellular matrix. Here, circular dichroism and fluorescence spectroscopic techniques are used to demonstrate that low molecular weight heparin and chondroitin sulfate cause significant changes in secondary and tertiary structure of IFN-gamma. The results suggest that heparin and chondroitin sulfate modulate IFN-gamma activity by causing structural changes in the IFN-gamma dimer.
Balasubramanian
V
V
Department of Pharmaceutics, State University of New York at Buffalo, 14260-1200, USA.
Ramanathan
M
M
D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000737:chemistry*; D002942:Circular Dichroism; D006025:Glycosaminoglycans / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry*; D007371:Interferon-gamma / Q000737:chemistry*; D011487:Protein Conformation; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence / Q000379:methods
D006025:Glycosaminoglycans; D006495:Heparin, Low-Molecular-Weight; D007371:Interferon-gamma; D002809:Chondroitin Sulfates