Double-resonance J-edited (1)H-NMR detection of 6-(13)C-D-2-deoxyglucose uptake in glioma cells.
The C6 methylene protons were selectively detected in (1)H-NMR spectra of intact glioma cells incubated with 6-(13)C-D-2-deoxyglucose (6-(13)C-2dG), a (13)C-enriched glucose analog that is suitable for monitoring glucose utilization in brain tumors. Spectral editing via (1)H-(13)C scalar coupling was performed with twin spin-echo double resonance (T-SEDOR), a pulse sequence which combines chemical specificity and high sensitivity, requires no solvent pre-saturation, and can easily be adapted to imaging protocols. This work demonstrates the suitability of the pulse sequence for monitoring 6-(13)C-2dG uptake in living cells in vitro, in spite of line-broadening and the occurrence of other strong signals in the spectral region of interest (3.5-4.4 ppm).
Knijn
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A
Laboratory of Cell Biology, Istituto Superiore di Sanità, 00161 Rome, Italy.
Casieri
C
C
Carpinelli
G
G
Testa
C
C
Podo
F
F
De Luca
F
F
D000818:Animals; D001692:Biological Transport; D002247:Carbon Isotopes; D003847:Deoxyglucose / Q000032:analysis* / Q000378:metabolism; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051381:Rats
D002247:Carbon Isotopes; D003847:Deoxyglucose