Loss of VLA-3 (CD49c/CD29) expression in two multidrug resistant Burkitt's lymphoma cell lines.
Expression of P-glycoprotein (P-gp) mediated multidrug resistance (MDR) has been suggested to be associated with an impaired clinical outcome in several malignancies. In contrast to P-gp itself, further phenotypical and functional alterations related to MDR are poorly characterized. In this in vitro study, we analyzed two Burkitt's lymphoma cell lines (Raji and Daudi) for the beta 1 integrin phenotype prior to and after induction of MDR via co-cultivation with vincristine. A significant loss of the VLA-3 (CD49c/CD29) adhesion receptor was observed whereas all other intergins analyzed lacked considerable changes. We conclude that induction of P-gp mediated MDR does not only affect resistance to cytotoxic drugs but also induces cellular changes with potential relevance for migratory and/or adhesive properties of malignant cells.
Duensing
S
S
Department of Pathology, Medizinische Hochschule Hannover, Germany.
Duensing
A
A
Grosse
J
J
Atzpodien
J
J
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis*; D002051:Burkitt Lymphoma / Q000276:immunology*; D018432:Drug Resistance, Multiple / Q000276:immunology*; D006801:Humans; D039422:Integrin alpha3; D039201:Integrin alpha3beta1; D019012:Integrin beta1 / Q000032:analysis*; D016023:Integrins / Q000032:analysis*; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000633:toxicity*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015703:Antigens, CD; D039422:Integrin alpha3; D039201:Integrin alpha3beta1; D019012:Integrin beta1; D016023:Integrins; D014750:Vincristine