Enhancement of brush border membrane peptidase activity in rat jejunum induced by starvation.
Conflicting results have been obtained in previous studies concerning the adaptation of intestinal blush border membrane enzymes to starvation. This study was designed to clarity the changes in these enzymes under starvation conditions, using a molecular biological approach. Sprague-Dawley rats were starved or given total parenteral nutrition (TPN) for 5 days. Rats allowed free access to food were used as controls. Changes in the activity and expression of jejunal brush border membrane enzymes were compared between three groups. In the starved group, aminopeptidase N and dipeptidyl peptidase IV activity was significantly elevated to 177% and 166%, respectively, of control values. In contrast, sucrase and maltase activity was significantly decreased. The activity of these peptidases also tended to be increased at the renal brush border membrane. Up-regulation of peptidase activity was not evident in the TPN group. Western and Northern blot analysis revealed that the changes in aminopeptidase N activity were attributable to increases in the protein and mRNA level. The activity and expression of brush border membrane peptidases in rat jejunum is up-regulated during starvation, and these changes are considered to be an effect of whole-body malnourishment, rather than an absence of luminal nutrition.
Ihara
T
T
2nd Department of Internal Medicine, Shiga University of Medical Science, Seta, Otsu, Japan.
Tsujikawa
T
T
Fujiyama
Y
Y
Ueyama
H
H
Ohkubo
I
I
Bamba
T
T
D000626:Aminopeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight; D018826:CD13 Antigens / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology*; D018819:Dipeptidyl Peptidase 4 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology / Q000254:growth & development; D007583:Jejunum / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008871:Microvilli / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013217:Starvation / Q000201:enzymology*; D013394:Sucrase-Isomaltase Complex / Q000235:genetics / Q000378:metabolism
D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D011691:Puromycin; D013394:Sucrase-Isomaltase Complex; D010447:Peptide Hydrolases; D000626:Aminopeptidases; C025579:enkephalin degrading enzyme; D018826:CD13 Antigens; D018819:Dipeptidyl Peptidase 4