title: Effects of a 98% solution of glycerol or sterilization with ethylene oxide on FeLV in bone allografts and effects on bone incorporation of allografts in cats.
abstract: OBJECTIVE
  To compare virucidal effects and bone incorporation properties of cortical bone allografts transplanted into specific-pathogen-free (SPF) cats. Allografts consisted of untreated bone from a SPF cat (negative-control group) and bone from 5 FeLV-infected cats that was subjected to sterilization with ethylene oxide (ETO), preservation with glycerol, or no treatment (positive-control group).
  
  
  METHODS
  Bones from the aforementioned groups and twenty 8-week-old SPF cats (5 cats/group) implanted with an allograft from 1 of the aforementioned groups.
  
  
  METHODS
  After implantation, blood samples were collected weekly to monitor FeLV p27 antigen and antibody titers. Quantification of FeLV provirus was performed on blood samples at weeks 0, 4, and 8 and donor bone samples at time of implantation. Cats were euthanatized 8 weeks after transplantation, and graft sites were evaluated.
  
  
  RESULTS
  All results for negative-control cats were negative. All ETO group cats had negative results for antigen and provirus in blood, whereas 1 cat had a low antibody titer. Although 3 ETO-treated allografts were positive for provirus, the DNA appeared denatured. One cat in the glycerol group had positive results for all tests in blood samples. All glycerol-preserved allografts were positive when tested for provirus. All results for positive-control group cats were positive. Differences in incorporation of bone grafts were not observed.
  
  
  CONCLUSIONS
  Glycerol preservation of FeLV-infected bone allografts did not eliminate transmission of retrovirus to recipients. In contrast, ETO sterilization appeared to denature DNA and prevent infection. Treatments did not affect incorporation of bone grafts in young cats.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824-1314, USA.
authors.forename: G S
authors.initials: GS
authors.lastname: Coronado
authors.forename: C L
authors.initials: CL
authors.lastname: Swenson
authors.forename: S A
authors.initials: SA
authors.lastname: Martinez
authors.forename: K S
authors.initials: KS
authors.lastname: Burkhardt
authors.forename: S P
authors.initials: SP
authors.lastname: Arnoczky
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood; D016025:Bone Transplantation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D002415:Cats / Q000601:surgery*; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005027:Ethylene Oxide / Q000737:chemistry / Q000494:pharmacology*; D005470:Fluorometry / Q000662:veterinary; D005990:Glycerol / Q000737:chemistry; D006651:Histocytochemistry; D005256:Leukemia Virus, Feline / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011859:Radiography; D011897:Random Allocation; D012192:Retroviridae Infections / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary; D013047:Specific Pathogen-Free Organisms; D013242:Sterilization / Q000379:methods*; D014184:Transplantation, Homologous / Q000662:veterinary; D014412:Tumor Virus Infections / Q000517:prevention & control / Q000635:transmission / Q000662:veterinary; D014457:Ulna / Q000000981:diagnostic imaging / Q000601:surgery
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D004202:Disinfectants; D005027:Ethylene Oxide; D005990:Glycerol