title: High-yield expression and purification of recombinant proteins in bacteria: a versatile vector for glutathione S-transferase fusion proteins containing two protease cleavage sites.
authors.affiliation: Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: B U
authors.initials: BU
authors.lastname: Sehgal
authors.forename: R
authors.initials: R
authors.lastname: Dunn
authors.forename: L
authors.initials: L
authors.lastname: Hicke
authors.forename: H A
authors.initials: HA
authors.lastname: Godwin
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D005822:Genetic Vectors; D005982:Glutathione Transferase / Q000235:genetics*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D010450:Endopeptidases