Rapid and reliable protein extraction from yeast.
The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces cerevisiae and for yeast Hansenula polymorpha DL-1. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates.
Kushnirov
V V
VV
Institute of Experimental Cardiology, Cardiology Research Centre, Moscow, Russia. vita@cardio.ru
D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009172:Mycology / Q000379:methods; D010843:Pichia / Q000737:chemistry* / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000254:growth & development
D005656:Fungal Proteins