title: Intestinal ischaemia-reperfusion increases plasma amylin concentration in rats.
abstract: Intestinal ischaemia-reperfusion and hyperamylinaemia are both associated with severe acute pancreatitis. The aim of this study was to examine the relationship between intestinal-ischaemia reperfusion and plasma amylin in an experimental model. Wistar rats (n = 24, 400-450 g) were divided into three groups: (1) a sham (S)-operated group (n = 7) that underwent laparotomy and isolation (without clamping) of the superior mesenteric artery, (2) an ischaemia-reperfusion (IR) group (n = 7) that had clamping of the superior mesenteric artery for 60 min followed by 15 min reperfusion, and (3) a control (C) group (n = 10) that underwent no surgery. Amylin was significantly elevated in the IR group (median 39 pM, range 30-44) compared with the S group (19 pM, range 15-45; Mann-Whitney U, p < 0.05) and the C group (24 pM, range 15-55; p < 0. 01). Insulin was significantly elevated in the IR group (2,060 pM, range 1,000-4,650) compared with the S group (558 pM, range 424-2, 020; p < 0.01). There was a significant positive correlation between amylin and insulin (R = 0.82, F = 46.6, p < 0.0001), but not between amylin and glucose or insulin and glucose. Intestinal histology was consistent with an ischaemia-reperfusion injury, whereas pancreatic histology was normal. The unique finding that plasma amylin concentration is increased with intestinal ischaemia-reperfusion injury warrants further investigation.
authors.affiliation: Pancreatitis Research Group, Department of Surgery, Faculty of Medicine and Health Science, University of Auckland, New Zealand. a.phillis@auckland.ac.nz
authors.forename: A R
authors.initials: AR
authors.lastname: Phillips
authors.forename: F M
authors.initials: FM
authors.lastname: Abu-Zidan
authors.forename: M J
authors.initials: MJ
authors.lastname: Bonham
authors.forename: M O
authors.initials: MO
authors.lastname: Simovic
authors.forename: G J
authors.initials: GJ
authors.lastname: Cooper
authors.forename: J A
authors.initials: JA
authors.lastname: Windsor
mesh_terms: D000682:Amyloid / Q000097:blood*; D000818:Animals; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D007511:Ischemia / Q000097:blood* / Q000150:complications / Q000473:pathology; D058228:Islet Amyloid Polypeptide; D008297:Male; D009994:Osmolar Concentration; D010195:Pancreatitis / Q000150:complications; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood*
chemical_list: D000682:Amyloid; D058228:Islet Amyloid Polypeptide