A homolog of mammalian antizyme is present in fission yeast Schizosaccharomyces pombe but not detected in budding yeast Saccharomyces cerevisiae.
The antizymes (AZ) are proteins that regulate cellular polyamine pools in metazoa. To search for remote homologs in single-celled eukaryotes, we used computer software based on hidden Markov models. The most divergent homolog detected was that of the fission yeast Schizosaccharomyces pombe. Sequence identities between S.POMBE: AZ and known AZs are as low as 18-22% in the most conserved C-terminal regions. The authenticity of the S.POMBE: AZ is validated by the presence of a conserved nucleotide sequence that, in metazoa, promotes a +1 programmed ribosomal frameshift required for AZ expression. However, no homolog was detected in the completed genome of the budding yeast Saccharomyces cerevisiae. Procedural details and supplementary information can be found at http://itsa.ucsf.edu/ approximately czhu/AZ.
Zhu
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Department of Microbiology and Immunology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Karplus
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Grate
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Coffino
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D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D004791:Enzyme Inhibitors / Q000737:chemistry; D005656:Fungal Proteins / Q000235:genetics*; D016681:Genome, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D065108:Ornithine Decarboxylase Inhibitors; D011506:Proteins; C031017:ornithine decarboxylase antizyme