title: Extraordinarily high density of unrelated genes showing overlapping and intraintronic transcription units.
abstract: The cloning of pyrroline 5-carboxylate reductase from Drosophila melanogaster was accomplished by cDNA complementation of an Escherichia coli proline auxotroph. The corresponding P5cr gene is tightly clustered with three other expressed coding regions. A bidirectional promoter, an overlapping 3'UTR and an intraintronic sequence may all be found in only 4.3 kb, making this the most densely clustered region of unrelated genes in any eukaryote.
authors.affiliation: Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6.
authors.forename: S R
authors.initials: SR
authors.lastname: Misener
authors.forename: V K
authors.initials: VK
authors.lastname: Walker
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics; D005807:Genes, Overlapping; D016678:Genome; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D011762:Pyrroline Carboxylate Reductases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase
chemical_list: D020413:3' Untranslated Regions; D011762:Pyrroline Carboxylate Reductases; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase