Analysis of carbohydrates using liquid chromatography--surface plasmon resonance immunosensing systems.
An immunosensing system based on surface plasmon resonance (SPR) was used for on-line detection and characterization of carbohydrate molecules separated by high-performance liquid chromatography. These analytes, with or without serum, were continuously separated and analyzed in the combined liquid chromatography-surface plasmon resonance (LC-SPR) system. By using weak and readily reversible monoclonal antibodies, the SPR system allowed specific on-line monitoring of the substances. To increase the specificity of the immunosensor, nonrelevant antibodies were used as reference in a serial flow cell. The sensitivity of the LC-SPR system was dependent on molecular weight of the carbohydrate, affinity of binding, and design of the sensor.
Jungar
C
C
Department of Physics and Measurement Technology, Linköping University, Sweden.
Strandh
M
M
Ohlson
S
S
Mandenius
C F
CF
D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods*
D002241:Carbohydrates