Rearrangement generated in double genes, NSP1 and NSP3, of viable progenies from a human rotavirus strain.
We generated rotavirus clones with rearrangement in vitro by serial passages of a human rotavirus strain (IGV-80-3) at high multiplicity of infection and determined nucleotide sequences of the rearranged genes from two distinct rotavirus clones, each of which possesses two rearranged genes: a common rearranged NSP1 gene and NSP3 gene with slightly different migration in polyacrylamide gel electrophoresis. Sequence analysis showed that the rearranged NSP1 and NSP3 genes had similar gene structures: concatemerization in a head to tail orientation and partial duplication of the open reading frame following the termination codon. The rearranged NSP1 gene had a direct repeat, whereas in the rearranged NSP3 gene, no such pattern was found.
Kojima
K
K
Department of Hygiene, Sapporo Medical University School of Medicine, Japan. kkojima@sapmed.ac.jp
Taniguchi
K
K
Kawagishi-Kobayashi
M
M
Matsuno
S
S
Urasawa
S
S
D015152:Blotting, Northern; D019976:Cloning, Organism; D015321:Gene Rearrangement; D005814:Genes, Viral; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics*; D017421:Sequence Analysis
D012367:RNA, Viral