title: Decreased in vitro fertilization and cleavage rates after an equipment error during CO(2) calibration.
abstract: OBJECTIVE
  To report the decreased IVF rate of oocytes and the reduced cleavage rate of pronuclear stage embryos after gas has backflowed from a gas analyzer into an incubator during the calibration of the CO(2) concentration.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Clinical research unit for reproductive medicine in a hospital.
  
  
  METHODS
  Backflow of gas from a gas analyzer into an incubator during calibration of the CO(2) concentration.
  
  
  METHODS
  The IVF rate of oocytes and the cleavage rate of pronuclear stage embryos.
  
  
  RESULTS
  Thirty-two oocytes were retrieved from four infertile female patients. Nine oocytes were fertilized and four fertilized oocytes were cleaved. In a related animal experiment, the cleavage rate was 57.1% (36/63) in the control group and 25.4% (16/63) in the study group (P =.00026).
  
  
  CONCLUSIONS
  The backflow of gas from the gas analyzer adversely affected the fertilization of oocytes and the cleavage of pronuclear stage embryos.
authors.affiliation: Clinical Research Unit for Reproductive Medicine, Shanxi Provincial Hospital, Taiyuan, People's Republic of China. hxwang@public.ty.sx.cn
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D002138:Calibration; D002245:Carbon Dioxide / Q000009:adverse effects; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D004868:Equipment Failure; D005260:Female; D005306:Fertilization / Q000187:drug effects; D005307:Fertilization in Vitro / Q000295:instrumentation*; D006801:Humans; D008297:Male; D051379:Mice
chemical_list: D002245:Carbon Dioxide