Proglucagon cDNAs from the leopard frog, Rana pipiens, encode two GLP-1-like peptides.
We have isolated and characterized proglucagon cDNAs from the intestine and pancreas of the leopard frog Rana pipiens. R. pipiens proglucagon encodes glucagon, glucagon-like peptides 2 (GLP-2), and two glucagon-like peptide 1 (GLP-1) like sequences. The pancreatic and intestinal cDNAs were of identical structure and sequence suggesting that, unlike many other non-mammalian vertebrates, there is little or no alternative splicing of the proglucagon mRNA in this species. A phylogenetic analysis of the GLP-1 encoding sequences implies that the exon encoding GLP-1 was triplicated early in frog evolution, more than 150 million years ago, before the divergence of modern frogs.
Irwin
D M
DM
Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Ont., Canada. david.irwin@utoronto.ca
Sivarajah
P
P
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D005934:Glucagon / Q000235:genetics*; D052216:Glucagon-Like Peptide 1; D053767:Glucagon-Like Peptide 2; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics*; D010455:Peptides / Q000235:genetics; D010802:Phylogeny; D052336:Proglucagon; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011894:Rana pipiens / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D053767:Glucagon-Like Peptide 2; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; D052336:Proglucagon; D052216:Glucagon-Like Peptide 1; D005934:Glucagon