Cellular proteins bind to the 3' and 5' untranslated regions of dengue 2 virus genome.
In vitro generated cloned full length dengue 2 virus untranslated regions (UTRs) were used in RNA gel mobility shift assays to examine cellular factors binding to the virus genomes. Cellular factors in lysates of Vero (monkey) and C6/36 (mosquito) cells bound specifically and non-specifically to the dengue 2 virus 3' UTR. Non-specific interaction with the 5' UTR, resulting in formation of at least 4 band shift complexes was noted with lysate of the C6/36 cells only. Pre-treating the cell lysates with proteinase K affected binding of cellular factors to the dengue 2 virus UTRs, suggesting that the cellular factors were proteins. These findings suggest that cellular proteins could interact with specific sites on the dengue virus genomes.
Ong
C C
CC
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Lam
S K
SK
AbuBakar
S
S
D020413:3' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D020121:5' Untranslated Regions / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D003716:Dengue Virus / Q000235:genetics*; D016679:Genome, Viral; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014709:Vero Cells
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017931:DNA Primers; D012367:RNA, Viral; D016601:RNA-Binding Proteins