title: Immunocytochemical evidence for a progesterone receptor in neurons and glial cells of the rat spinal cord.
abstract: Using the KC 146 monoclonal antibody recognizing the B-form of the progesterone receptor (PR) and immunocytochemical techniques, we investigated if PR-immunoreactive cells are present in the rat spinal cord. Neurons from ventral horn Lamina IX, glial cells in gray and white matter and ependymal cells were PR-positive. Evidence for estrogen-inducibility of PR in ovariectomized rats was not observed. There were no significant gender differences in neuronal PR immunostaining intensity in the spinal cord, measured by computerized image analysis. In pituitary and uterus from estrogenized female rats, PR showed a strict nuclear localization, whereas in neurons and glial cells of the spinal cord, PR localized in cytoplasm and/or nucleus and in some cell processes. This receptor may be implicated in some of the biological effects of progesterone described in the spinal cord.
authors.affiliation: Laboratory of Neuroendocrine Biochemistry, Instituto de Biologia y Medicina Experimental, Department of Biochemistry, University of Buenos Aires, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Labombarda
authors.forename: R
authors.initials: R
authors.lastname: Guennoun
authors.forename: S
authors.initials: S
authors.lastname: Gonzalez
authors.forename: P
authors.initials: P
authors.lastname: Roig
authors.forename: A
authors.initials: A
authors.lastname: Lima
authors.forename: M
authors.initials: M
authors.lastname: Schumacher
authors.forename: A F
authors.initials: AF
authors.lastname: De Nicola
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009457:Neuroglia / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D011980:Receptors, Progesterone / Q000032:analysis* / Q000276:immunology; D012737:Sex Factors; D013116:Spinal Cord / Q000737:chemistry* / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D004967:Estrogens; D011980:Receptors, Progesterone