title: Cytocidal and apoptotic effects of the ClyA protein from Escherichia coli on primary and cultured monocytes and macrophages.
abstract: Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates a hemolytic phenotype. We show here that highly purified ClyA and ClyA-expressing E. coli were cytotoxic and apoptogenic to fresh as well as cultured human and murine monocytes/macrophages.
authors.affiliation: Department of Microbiology, Umeå University, S-901 87 Umeå, Sweden.
authors.forename: X H
authors.initials: XH
authors.lastname: Lai
authors.forename: I
authors.initials: I
authors.lastname: Arencibia
authors.forename: A
authors.initials: A
authors.lastname: Johansson
authors.forename: S N
authors.initials: SN
authors.lastname: Wai
authors.forename: J
authors.initials: J
authors.lastname: Oscarsson
authors.forename: S
authors.initials: S
authors.lastname: Kalfas
authors.forename: K G
authors.initials: KG
authors.lastname: Sundqvist
authors.forename: Y
authors.initials: Y
authors.lastname: Mizunoe
authors.forename: A
authors.initials: A
authors.lastname: Sjöstedt
authors.forename: B E
authors.initials: BE
authors.lastname: Uhlin
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D053938:DNA Fragmentation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000235:genetics / Q000302:isolation & purification / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D009000:Monocytes / Q000187:drug effects* / Q000473:pathology; D020298:U937 Cells
chemical_list: D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C119944:hlyE protein, E coli