Size exclusion chromatography does not require pores.
Separation of macromolecules on the basis of their molecular weight by size exclusion chromatography has long been considered to be caused by the geometry-dependent partition of macromolecules between a continuous phase and the porous interior of a gel or cross-linked bead. The volume of a pore accessible to a solute is limited by its relative dimensions, so larger molecules will have access to a smaller volume and will remain in a bead for a shorter time than smaller solutes. Our recent alternate picture proposes that the partition coefficient can be calculated from a thermodynamic model for the free energy of mixing of the solute with the gel phase. Size-dependent exclusion caused by the unfavorable entropy of mixing associated with the partition is predicted; the magnitude of the effect is modified by enthalpic interactions between the solute and the gel phase. This concept is extended here to describe the partition of macromolecules into a layer of terminally attached polymer chains grafted onto a solid bead. Both simple mean field and self-consistent field theory calculations predict size-dependent entropic exclusion. Experimental results obtained with neutral polymer chains grafted onto solid polystyrene latex beads confirm the predictions.
Brooks
D E
DE
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada V6T 2B5. don@pathology.ubc.ca
Haynes
C A
CA
Hritcu
D
D
Steels
B M
BM
Müller
W
W
D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D010316:Particle Size; D011108:Polymers
D011108:Polymers