title: PrP immunohistochemistry: different protocols, including a procedure for long formalin fixation, and a proposed schematic classification for deposits in sporadic Creutzfeldt-Jakob disease.
abstract: The use of immunohistochemistry on formalin-fixed and paraffin-embedded tissue has greatly improved the neuropathological diagnosis of Creutzfeldt-Jakob disease and the other subacute spongiform encephalopathies in human and animals. Two pitfalls of this technique, however, currently exist: low sensitivity after long formalin fixation and difficulties in interpreting some images. Here we review the protocols currently in use for the pretreatment of sections allowing PrP detection by immunohistochemistry. In addition, a technique useful after long formalin fixation is reported: enzymatic digestion with proteinase K (24 degrees C, 1/100 for 8 minutes) was employed in addition to the usual autoclaving (121 degrees C for 10 minutes) followed by formic acid (99% for 5 minutes) and 4M guanidine thiocyanate (4 degrees C for 2 hours). This allowed a substantial increase in the sensitivity of 3F4 immunohistochemistry on paraffin-embedded tissue, especially after prolonged formalin fixation. In addition, we suggest a simple method for classification of PrP immunolabelling in sporadic Creutzfeldt-Jakob disease that would allow easy comparisons.
authors.affiliation: Raymond Escourolle Neuropathology Laboratory, La Salpêtrière Hospital, Pierre et Marie Curie, Paris VI University, INSERM U 360, Association Claude Bernard, 75651 Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Privat
authors.forename: V
authors.initials: V
authors.lastname: Sazdovitch
authors.forename: D
authors.initials: D
authors.lastname: Seilhean
authors.forename: J L
authors.initials: JL
authors.lastname: LaPlanche
authors.forename: J J
authors.initials: JJ
authors.lastname: Hauw
mesh_terms: D001921:Brain / Q000378:metabolism / Q000473:pathology; D007562:Creutzfeldt-Jakob Syndrome / Q000378:metabolism / Q000473:pathology*; D019286:Endopeptidase K; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding; D011328:Prions / Q000032:analysis*; D012680:Sensitivity and Specificity; D016707:Tissue Fixation / Q000379:methods
chemical_list: D005404:Fixatives; D011328:Prions; D005557:Formaldehyde; D019286:Endopeptidase K