title: Expression of luteinising hormone and chorionic gonadotropin beta-subunit messenger-RNA and protein in human peripheral blood leukocytes.
abstract: Some pituitary hormones are expressed in leukocytes and are thought to play a role in the regulation of leukocyte function. We studied the expression of the mRNA for the beta-chains of luteinising hormone (LHbeta) and chorionic gonadotropin (CGbeta) and their translation into protein in various leukocyte subsets. Monocytes, granulocytes, B and T-cells from peripheral blood were separated. Lymphocytes were stimulated with various mitogens, prolactin and mixed lymphocyte culture. LHbeta and CGbeta mRNA expression was determined by reverse transcriptase polymerase chain reaction. LH, LHbeta, CG and CGbeta protein were determined in the culture medium by immunofluorometric assays. LHbeta mRNA expression was detected in all cell fractions and cultures and stimulation with prolactin induced LH protein in the culture medium. CGbeta mRNA expression appeared after culture of lymphocytes, but mitogens and prolactin had no clear stimulating effect. The LH expression in leukocytes shown here suggests an autocrine function of this hormone in blood cells.
authors.affiliation: Department of Clinical Chemistry, Helsinki University Central Hospital, Finland. kristina.hotakainen@helsinki.fi
authors.forename: P K
authors.initials: PK
authors.lastname: Hotakainen
authors.forename: E M
authors.initials: EM
authors.lastname: Serlachius
authors.forename: S I
authors.initials: SI
authors.lastname: Lintula
authors.forename: H V
authors.initials: HV
authors.lastname: Alfthan
authors.forename: J P
authors.initials: JP
authors.lastname: Schröder
authors.forename: U E
authors.initials: UE
authors.lastname: Stenman
mesh_terms: D000328:Adult; D001483:Base Sequence; D002460:Cell Line; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics* / Q000378:metabolism
chemical_list: D018997:Chorionic Gonadotropin, beta Subunit, Human; D017931:DNA Primers; D012333:RNA, Messenger; D007986:Luteinizing Hormone