title: Proteomic analysis of colonic crypts from normal, multiple intestinal neoplasia and p53-null mice: a comparison with colonic polyps.
abstract: In order to observe cellular changes caused by mutation of the tumor suppressors, APC and p53, we have generated protein expression profiles of mouse colon epithelial cells using two-dimensional electrophoresis (2-DE). Crypts, polyps and stroma were isolated from normal, multiple intestinal neoplasia (MIN) and p53-null mice, each with a C57Black/6J background, and subjected to 2-DE in two separate pH ranges (pH 3-10 and pH 6-11). No significant differences in protein expression patterns were observed between the normal, MIN and p53-null colon epithelial crypts. However, 64 proteins from the MIN polyps showed a 2-fold or greater difference in intensity that was statistically significant as assessed by the Wilcoxon rank-sum test (p < or = 0.05). Of these, calreticulin, carbonic anhydrase I and a new member of the glutathione-S-transferase theta family of proteins have so far been identified using an in-gel digestion protocol coupled with reversed-phase high performance liquid chromatography (RP-HPLC) ion-trap mass spectrometry. In addition, 38 marker proteins have been identified in a continuing effort to generate a comprehensive 2-DE database of proteins expressed by mouse colon epithelial cells (these databases are available at http://www.ludwig.edu.au/jpsl/jpslhome. html).
authors.affiliation: Ludwig Institute for Cancer Research, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
authors.forename: A R
authors.initials: AR
authors.lastname: Cole
authors.forename: H
authors.initials: H
authors.lastname: Ji
authors.forename: R J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D000818:Animals; D003106:Colon / Q000737:chemistry* / Q000473:pathology; D003111:Colonic Polyps / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007414:Intestinal Neoplasms / Q000737:chemistry* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009363:Neoplasm Proteins / Q000032:analysis*; D009378:Neoplasms, Multiple Primary / Q000737:chemistry* / Q000473:pathology; D020543:Proteome / Q000032:analysis*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology*
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome; D016159:Tumor Suppressor Protein p53