Effects of intraoral splint wear on proteoglycans in the temporomandibular joint disc.
Intraoral splints are a common dental treatment for dysfunctions of the temporomandibular joint (TMJ), but their effects on the structures of the joint, specifically the disc, have not been well investigated. This study examined proteoglycans (PGs) of the TMJ disc of the miniature pig and tested for alterations resulting from intraoral splint wear. Sixteen female pigs were divided into three groups: control (C), control splint (CS), and protrusive splint (PS). Splinted groups received chrome-cobalt ramp splints which were worn continuously for 2 months. PG content within various disc locations was determined by colorimeteric assay. PG synthesis and type were examined by labeling with (35)S-sulfate and SDS-PAGE analysis. Average water content of the disc was 77.1%, which places it at the high end of the normal range for collagenous biomaterials (60-80%). PGs migrating to the positions typical of aggrecan, biglycan, and decorin on SDS-PAGE were present in all locations of all groups. The highest content and synthesis of PGs were always found in the intermediate band of the disc regardless of group (P < 0.05), supporting the notion that this band encounters heavy compressive loading during function. The joints of animals from both splinted groups showed a high frequency of gross pathology. Biglycan synthesis was increased in both splinted groups (P < 0.05). Newly synthesized biglycan had a shorter migration distance in the intermediate bands of the CS group, suggesting increased hydrodynamic size. These findings suggest that intraoral splint wear may cause disc damage or remodeling.
Sindelar
B J
BJ
Department of Bioengineering, University of Washington, Seattle, Washington, 98195-7446, USA. sindelar@u.washington.edu
Evanko
S P
SP
Alonzo
T
T
Herring
S W
SW
Wight
T
T
D000818:Animals; D058578:Biglycan; D058575:Decorin; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D007592:Joint Diseases / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D008335:Mandibular Condyle / Q000473:pathology; D017090:Occlusal Splints; D011509:Proteoglycans / Q000096:biosynthesis / Q000378:metabolism*; D013314:Stress, Mechanical; D013431:Sulfates / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature; D019224:Temporomandibular Joint Disc / Q000378:metabolism* / Q000473:pathology
C546884:BGN protein, human; D058578:Biglycan; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates