Fate of meiotic lamin C2 in rat spermatocytes cultured in the presence of okadaic acid.
The fate of nuclear envelope proteins during the pachytene/metaphase I transition was investigated in rat spermatocytes cultured in vitro in the presence of the phosphatase inhibitor okadaic acid (OA). Under these experimental conditions lamin B1 and the lamina-associated proteins 2 (LAPs2) behave as already described in other cell types. In contrast to these results, meiotic lamin C2 appears to be degraded after addition of OA to the spermatocyte culture medium as this lamin was no longer detectable by immunofluorescence microscopy or by immunoblotting. Taking into account the peculiarities of the lamin C2 primary structure, it is tempting to speculate that degradation of this protein represents a critical step in the process of disassembly of the spermatocyte nuclear envelope.
von Glasenapp
E
E
Department of Cell and Developmental Biology, Biocenter, University of Würzburg, Germany.
Benavente
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D034921:Lamin Type B; D007797:Laminin; D034882:Lamins; D008297:Male; D008540:Meiosis / Q000187:drug effects*; D008565:Membrane Proteins / Q000378:metabolism; D008677:Metaphase / Q000187:drug effects; D008954:Models, Biological; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D019319:Okadaic Acid / Q000494:pharmacology*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D013090:Spermatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013573:Synaptonemal Complex / Q000187:drug effects
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D034921:Lamin Type B; D007797:Laminin; D034882:Lamins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C085453:Sycp3 protein, rat; C087355:lamin C2; C093413:lamina-associated polypeptide 2; D019319:Okadaic Acid