The mouse ERG before and after light damage is independent of p53.
Death of retinal photoreceptors by apoptosis is observed under many physiological and pathological conditions such as histogenesis, retinal dystrophies and light-induced photoreceptor degeneration. To date, little is known about regulatory mechanisms for apoptosis in the retina. The tumor suppressor gene p53 is a regulator of apoptosis in a number of systems, however, p53-independent apoptosis has also been described. We have therefore investigated whether the lack of p53 influences the dark-adapted ERG in C57BL/6 p53-/- mice compared to p53+/+ control littermates under physiological (regular light-dark cycle) conditions. We also recorded ERGs at 12 to 14 h in darkness following diffuse bright light exposure to 8,000 or 15,000 lux for 2 h. ERG analysis over a range of 6 logarithmic units of light intensity revealed normal and virtually identical a-, b-, c-waves and oscillatory potentials in dark-adapted p53+/+ and p53-/- mice. After exposure to diffuse white fluorescent light strong decreases of all ERG components were found to be very similar in both genotypes. These data support the notion that the p53 protein is neither essential for normal retinal function nor for processes involved in light-induced depression of the ERG in mice.
Lansel
N
N
Dept of Ophthalmology, University Hospital, Zurich. Switzerland.
Hafezi
F
F
Marti
A
A
Hegi
M
M
Remé
C
C
Niemeyer
G
G
D000818:Animals; D003623:Dark Adaptation; D004596:Electroretinography / Q000528:radiation effects*; D005260:Female; D016158:Genes, p53 / Q000502:physiology*; D008027:Light / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology / Q000528:radiation effects*; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000235:genetics / Q000503:physiopathology*; D012162:Retinal Degeneration / Q000209:etiology / Q000235:genetics / Q000503:physiopathology*