title: Clinical application of amniotic membranes on a patient with epidermolysis bullosa.
abstract: The case of a patient with dystrophic epidermolysis bullosa treated with radiosterilised amniotic membranes is presented. The disorder is a congenital disease characterised by a poor desmosomal junction in the keratinocyte membrane. After proper donor screening, amnios were collected at Hospital Central Sur de Alta Especialidad (HCSAE), PEMEX and microbiological analysis was performed at Universidad Nacional Autónoma de México, FQUNAM, (Biology Dept. of the Chemistry Faculty, National Autonomous University of Mexico), before and after radiation sterilisation. Processing, packaging and sterilisation were performed at Instituto Nacional de Investigaciones Nucleares, ININ, (National Nuclear Research Institute). The patient, a ten-year-old boy with severe malnutrition, extensive loss of skin and pseudomonad infection in the whole body, was treated with gentle debridement in a Hubbard bath. Later amnion application was performed with sterilised amnios by using two different processes, in one of which the amnion was sterilised with paracetic acid, preserved in glycerol, kindly donated by the German Institute for Tissue and Cell Replacement and applied by Dr. Johannes C. Bruck, IAEA visiting expert, and the other amnion was processed at ININ: air dried and sterilised by gamma radiation at dose of 30 kGy. After spontaneous epithelisation was successfully promoted for seven days, the pain was alleviated and mobility was improved in a few hours and the patient's general condition was so improved that in a month he was discharged. Unfortunately, because this disease is revertive and has malignant degeneration, the prognosis is not good.
authors.affiliation: Instituto Nacional de Investigaciones Nucleares, México, D.F. memp@nuclear.inin.mx
authors.forename: M E
authors.initials: ME
authors.lastname: Martínez Pardo
authors.forename: M L
authors.initials: ML
authors.lastname: Reyes Frías
authors.forename: L E
authors.initials: LE
authors.lastname: Ramos Durón
authors.forename: E
authors.initials: E
authors.lastname: Gutiérrez Salgado
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez
authors.forename: M A
authors.initials: MA
authors.lastname: Marín
authors.forename: D
authors.initials: D
authors.lastname: Luna Zaragoza
mesh_terms: D000650:Amnion / Q000528:radiation effects; D001684:Biological Dressings; D002648:Child; D016108:Epidermolysis Bullosa Dystrophica / Q000473:pathology / Q000601:surgery*; D006801:Humans; D008297:Male; D016038:Skin Transplantation / Q000379:methods; D013242:Sterilization / Q000379:methods; D014182:Transplantation, Autologous