Increased high-density lipoprotein-3 binding to leukocytes following weight loss and improved glycemic control in type 2 diabetic patients.
This study evaluates the effect on high-density lipoprotein (HDL) binding activity in cultured granulocytes before and after metabolic control of non-insulin-dependent diabetes mellitus ([NIDDM] type 2 diabetes) patients. In 20 type 2 diabetic patients, diabetic control was accomplished by administration of oral antidiabetic agents and dietary restrictions. Adequate metabolic control was reflected by a decrease in the fasting glucose, glycosylated hemoglobin (HbA1c), mean insulin, and body mass index (BMI). After control of the diabetes, the mean HDL3 cholesterol was increased from 0.918 +/- 0.05 to 1.008 +/- 0.05 mmol/L (P < .05) and apolipoprotein AI (apo AI) was increased from 103 +/- 5.8 to 115 +/- 5.1 mg/dL (P < .01). The HDL3 maximum specific binding was higher after versus before diabetic control, 77 +/- 6 versus 122 +/- 8 ng/mg cell protein (P < .01). This increase was related to an increase in maximum binding ([Bmax] from 4.97 x 10(-10) to 8.3 x 10(-10) mol/L, P < .001), and no significant changes were observed in the Kd (from 1.47 x 10(-7) v 2.04 x 10(-7) mol/L). These results suggest that the metabolic control of type 2 diabetes increases HDL3 binding activity.
Paniagua
J A
JA
Unidad de Lipidos y Arteriosclerosis, Hospital Universitario Reina Sofia, Cordoba, Spain.
López-Miranda
J
J
Jansen
S
S
Zambrana
J L
JL
López Segura
F
F
Jiménez Perepérez
J A
JA
Pérez-Jiménez
F
F
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002352:Carrier Proteins; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D008055:Lipids / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood*; D053440:Lipoproteins, HDL3; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D016601:RNA-Binding Proteins; D018110:Receptors, Lipoprotein / Q000097:blood
D001786:Blood Glucose; D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D008055:Lipids; D008075:Lipoproteins, HDL; D053440:Lipoproteins, HDL3; D016601:RNA-Binding Proteins; D018110:Receptors, Lipoprotein; C031616:high density lipoprotein receptors; C075197:high density lipoprotein binding protein