The annexin A3-membrane interaction is modulated by an N-terminal tryptophan.
The crystal structure of annexin A3 (human annexin III) solved recently revealed a well-ordered folding of its N-terminus with the side chain of tryptophan 5 interacting with residues at the extremity of the central pore. Since the pore of annexins has been suggested as the ion pathway involved in membrane permeabilization by these proteins, we investigated the effect of the N-terminal tryptophan on the channel activity of annexin A3 by a comparative study of the wild-type and the W5A mutant in structural and functional aspects. Calcium influx and patch-clamp recordings revealed that the mutant exhibited an enhanced membrane permeabilization activity as compared to the wild-type protein. Analysis of the phospholipid binding behavior of wild-type and mutant protein was carried out by cosedimentation with lipids and inhibition of PLA(2) activity. Both methods reveal a much stronger binding of the mutant to phospholipids. The structure is very similar for the wild-type and the mutant protein. The exchange of the tryptophan for an alanine results in a disordered N-terminal segment. Urea-induced denaturation of the wild-type and mutant monitored by intrinsic fluorescence indicates a separate unfolding of the N-terminal region which occurs at lower urea concentrations than unfolding of the protein core. We therefore conclude that the N-terminal domain of annexin A3, and especially tryptophan 5, is involved in the modulation of membrane binding and permeabilization by annexin A3.
Hofmann
A
A
Max Planck Institut für Biochemie, D-82152 Planegg-Martinsried, Germany. hofmanna@ncifcrf.gov
Raguénès-Nicol
C
C
Favier-Perron
B
B
Mesonero
J
J
Huber
R
R
Russo-Marie
F
F
Lewit-Bentley
A
A
D000409:Alanine / Q000235:genetics / Q000378:metabolism; D017318:Annexin A3 / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D004594:Electrophysiology; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D008566:Membranes / Q000378:metabolism / Q000502:physiology; D008958:Models, Molecular; D010539:Permeability; D010743:Phospholipids / Q000378:metabolism*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D014364:Tryptophan / Q000235:genetics / Q000378:metabolism*; D014508:Urea / Q000737:chemistry
D008081:Liposomes; D010743:Phospholipids; D014364:Tryptophan; D014508:Urea; D017318:Annexin A3; D000409:Alanine; D002118:Calcium