Septal localization of the membrane-bound division proteins of Bacillus subtilis DivIB and DivIC is codependent only at high temperatures and requires FtsZ.
Using immunofluorescence microscopy, we have examined the dependency of localization among three Bacillus subtilis division proteins, FtsZ, DivIB, and DivIC, to the division site. DivIC is required for DivIB localization. However, DivIC localization is dependent on DivIB only at high growth temperatures, at which DivIB is essential for division. FtsZ localization is required for septal recruitment of DivIB and DivIC, but FtsZ can be recruited independently of DivIB. These localization studies suggest a more specific role for DivIB in division, involving interaction with DivIC.
Katis
V L
VL
Department of Biochemistry, University of Sydney, New South Wales 2006, Australia.
Wake
R G
RG
Harry
E J
EJ
D001412:Bacillus subtilis / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism*; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C085861:DivIB protein, Bacillus subtilis; C110609:DivIC protein, Bacillus subtilis; C071525:FtsZ protein, Bacteria; D008565:Membrane Proteins