title: Regeneration of Class III furcation defects with basic fibroblast growth factor (b-FGF) associated with GTR. A descriptive and histometric study in dogs.
abstract: BACKGROUND
  The poor predictability of periodontal regenerative treatment of Class III furcation defects stimulates the study of alternatives to improve its results, such as the use of polypeptide growth factors. The objective of this study was to evaluate, both histologically and histometrically, the effects of topical application of basic fibroblast growth factor (b-FGF) associated with guided tissue regeneration (GTR) in the treatment of Class III defects surgically induced in dogs.
  
  
  METHODS
  All second and fourth premolars of 5 mongrel dogs were used and randomly assigned to one of three treatment groups: group 1 (control), treated with scaling and root planing, tetracycline hydrochloride (125 mg/ml) conditioning, and GTR with a collagen membrane; group 2, same treatment as group 1 plus 0.5 mg of b-FGF; group 3, same treatment as group 1 plus 1.0 mg of b-FGF. After a 90-day healing period, routine histologic processing and staining with hematoxylin and eosin and Masson trichrome were performed.
  
  
  RESULTS
  The descriptive analysis indicated better regenerative results in both groups treated with b-FGF while the histometric data, analyzed by means of analysis of variance (ANOVA), showed greater filling of the defects in group 2 in comparison to the defects in groups 3 and 1, respectively, which was represented by a smaller area of plaque-occupied space (P = 0.004) as well as a greater amount of newly formed cementum (P = 0.002).
  
  
  CONCLUSIONS
  These results indicate that b-FGF, especially in smaller doses, may enhance the regenerative results in Class III furcation lesions, leading to greater filling of these defects with both mineralized and non-mineralized tissues.
authors.affiliation: Department of Periodontology, Araraquara Dental School, State University of São Paulo (UNESP), Brazil. crossajr@foar.unesp.br
authors.forename: C
authors.initials: C
authors.lastname: Rossa
authors.forename: E
authors.initials: E
authors.lastname: Marcantonio
authors.forename: J A
authors.initials: JA
authors.lastname: Cirelli
authors.forename: R A
authors.initials: RA
authors.lastname: Marcantonio
authors.forename: L C
authors.initials: LC
authors.lastname: Spolidorio
authors.forename: J C
authors.initials: JC
authors.lastname: Fogo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003739:Dental Cementum / Q000187:drug effects*; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology / Q000627:therapeutic use*; D017823:Furcation Defects / Q000188:drug therapy* / Q000601:surgery; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D015999:Multivariate Analysis; D011897:Random Allocation; D018709:Statistics, Nonparametric
chemical_list: D016222:Fibroblast Growth Factor 2