title: Synthesis of glycosylated polyethylenimine with reduced toxicity and high transfecting efficiency.
abstract: A safe and efficient synthesis of glycosylated polyethylenimine using titanium (IV) isopropoxide and sodium borohydride has been carried out as a substitute for the highly toxic sodium cyanoborohydride method currently used. Poryplexes formed between DNA and the various glycosylated polyethylenimines appeared to be much less cytotoxic than polyethylenimine (PEI)/DNA polyplexes.
authors.affiliation: Laboratoire de Chimie Bioorganique et Biotechnologie Moleculaire et cellulaire, Unité Mixte ENSCP/CNRS/AVENTIS (UMR 7001), ENSCP, Paris, France. fralecle@ext.jussieu.fr
authors.forename: F
authors.initials: F
authors.lastname: Leclercq
authors.forename: C
authors.initials: C
authors.lastname: Dubertret
authors.forename: B
authors.initials: B
authors.lastname: Pitard
authors.forename: D
authors.initials: D
authors.lastname: Scherman
authors.forename: J
authors.initials: J
authors.lastname: Herscovici
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D006031:Glycosylation; D011094:Polyethyleneimine / Q000138:chemical synthesis* / Q000494:pharmacology* / Q000633:toxicity; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D011094:Polyethyleneimine