title: [The determination of lipopolysaccharide admixtures in protein preparations].
abstract: A new method for the determination of lipopolysaccharide (LPS) admixtures in protein solutions has been developed. The method includes the periodate oxidation of LPS, biotinylation with biotin hydraside, immobilization on a nitrocellulose membrane and the development of biotinylated LPS in the streptavidin--alkaline phosphatase system. Proteins are previously removed from the solution by treatment with hot phenol. Development with the use of 5-bromoinodyl phosphate and nitrotetrazolium blue makes it possible to detect about 30 pg of LPS immobilized on the nitrocellulose membrane.
authors.affiliation: Research Institute for Design and Technology of Biologically Active Substances, State Scientific Center of Virology and Biotechnology Vector, Berdsk, Novosibirsk Region, Russia.
authors.forename: L Ia
authors.initials: LIa
authors.lastname: Denisova
authors.forename: I I
authors.initials: II
authors.lastname: Baturina
authors.forename: A I
authors.initials: AI
authors.lastname: Zakabunin
authors.forename: G N
authors.initials: GN
authors.lastname: Afinogenova
authors.forename: N M
authors.initials: NM
authors.lastname: Pustoshilova
mesh_terms: D019921:Biotinylation; D002138:Calibration; D003101:Collodion; D004340:Drug Contamination; D004926:Escherichia coli; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000032:analysis*; D008567:Membranes, Artificial; D010504:Periodic Acid; D011994:Recombinant Proteins / Q000032:analysis*; D012996:Solutions
chemical_list: D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D012996:Solutions; D010504:Periodic Acid; D003101:Collodion; C009288:metaperiodate
vernacular_title: Opredelenie primeseĭ lipopolisakharidov v preparatakh belkov.