Higher-order structure of nucleosome oligomers from short-repeat chromatin.
Sedimentation measurements and electron microscopy at a series of ionic strengths suggest that chromatin from neurons of the cerebral cortex is able to form condensed structures in vitro that are probably several turns of a solenoid with about six nucleosomes per turn. Since neuronal chromatin has a short nucleosomal repeat (approximately 165 bp) allowing virtually no linker DNA between nucleosomes, and yet forms apparently 'normal' elements of solenoid, the packing of nucleosomes in the solenoid must be highly constrained. This permits only a limited number of possible models, and enables tentative suggestions to be made about the location of the linker DNA in the typical solenoid.
Pearson
E C
EC
Department of Biochemistry, University of Cambridge, UK.
Butler
P J
PJ
Thomas
J O
JO
D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000166:cytology; D002843:Chromatin / Q000648:ultrastructure; D008854:Microscopy, Electron; D009474:Neurons; D009707:Nucleosomes / Q000235:genetics* / Q000648:ultrastructure; D009994:Osmolar Concentration; D012091:Repetitive Sequences, Nucleic Acid
D002843:Chromatin; D009707:Nucleosomes