Characterization of messenger RNA expression of estrogen receptor-alpha and -beta in patients with ovarian endometriosis.
OBJECTIVE
To evaluate the expression of estrogen receptor (ER)-alpha and ER-beta messenger RNA (mRNA) in ovarian endometriosis.


METHODS
Prospective study.


METHODS
University hospital.


METHODS
Patients with endometriosis and patients with uterine leiomyoma or carcinoma in situ.


METHODS
A sample of ovarian endometriotic cyst tissue was obtained from each of the 32 patients during laparoscopic cystectomy. Samples of ovarian tissue and endometrium were obtained from 15 patients during or just after surgery.


METHODS
Expression of mRNA for ER-alpha and ER-beta with use of the reverse transcription-polymerase chain reaction (RT-PCR) and nonradioactive in situ hybridization (ISH) techniques.


RESULTS
Expression of mRNA for ER-alpha and ER-beta was observed in all of the control tissues from the normal endometrium and normal ovaries in the RT-PCR and ISH analyses, although the distribution of positive signals changed in the ISH analysis during different phases of the menstrual cycle. Messenger RNA for ER-alpha was detected in all of the ovarian endometriotic cysts analyzed (19 of 19), but mRNA for the ER-beta was limited (12 of 19) in the RT-PCR analysis. The ISH analysis confirmed the RT-PCR results and revealed that the two estrogen receptors were localized in both epithelial and stromal cells of endometriotic tissues.


CONCLUSIONS
The results suggest that predominant expression of ER-alpha in both glandular epithelial and stromal cells may be essential to the development and growth of ovarian endometriosis.
Matsuzaki
S
S
Tohoku University School of Medicine, Sendai, Japan. matsuzaki@ob-gy.med.tohoku.ac.jp
Fukaya
T
T
Uehara
S
S
Murakami
T
T
Sasano
H
H
Yajima
A
A
D000328:Adult; D002278:Carcinoma in Situ / Q000378:metabolism; D003560:Cysts / Q000378:metabolism; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000378:metabolism; D008597:Menstrual Cycle / Q000378:metabolism; D010049:Ovarian Diseases / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D011446:Prospective Studies; D012333:RNA, Messenger / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000235:genetics*; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014594:Uterine Neoplasms / Q000378:metabolism
D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011960:Receptors, Estrogen