Rapid up-regulation of P2Y messengers during granulocytic differentiation of HL-60 cells.
HL-60 cells are human promyelocytic cells expressing two ATP receptors: the P2Y(2) and P2Y(11) subtypes. Our Northern blotting experiments have shown that P2Y(2) and P2Y(11) messengers were up-regulated in these cells, rapidly and independently of protein synthesis, following treatment with granulocytic differentiating agents such as retinoic acid, dimethylsulfoxide, granulocyte-colony stimulating factor, dibutyryl cyclic AMP and ATP. AR-C67085 and adenosine 5'-O-(3-thiotriphosphate), two potent agonists of the recombinant P2Y(11) receptor, increased intracellular cAMP concentration in HL-60 cells more potently than ATP itself. These observations support the conclusion that the effect of ATP on HL-60 cell differentiation is mediated by the P2Y(11) receptor.
Communi
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Institute of Interdisciplinary Research, School of Medicine, Universit¿e Libre de Bruxelles, Brussels, Belgium. communid@ulb.ac.be
Janssens
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Robaye
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Zeelis
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Boeynaems
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JM
D002454:Cell Differentiation; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis*; D015854:Up-Regulation
D012333:RNA, Messenger; D018048:Receptors, Purinergic P2