title: Trimerization of cell adhesion molecule L1 mimics clustered L1 expression on the cell surface: influence on L1-ligand interactions and on promotion of neurite outgrowth.
abstract: Several studies indicate that cell adhesion molecules have to be clustered on the cell surface to engage in adhesive functions. We investigated adhesive functions of clustered versus monomeric L1 extracellular parts in vitro to distinguish how clustering affects ligand binding and promotion of neurite outgrowth. Trimeric L1 was recombinantly expressed and covalently assembled by the cartilage matrix protein's coiled-coil domain. Trimeric L1 has an apparent molecular mass of approximately 380 kDa in the nonreduced form and approximately 130 kDa in the reduced form. Rotary shadowing electron micrographs of trimeric L1 revealed a rod-like shape terminating in three globular domains. Monomeric L1 assumes a horseshoe shape of domains Ig I-IV followed by a rod-like structure consisting of Ig V and VI and fibronectin type III 1-5. Circular dichroism measurements showed that the secondary structure consists of beta-sheets. Trimeric L1 binds to itself, to monomeric L1, to laminin-1, and to alpha5beta1 integrin in a concentration-dependent manner. In contrast, binding of monomeric L1 could only be saturated with itself but not with laminin-1 and with alpha5beta1 integrin. Promotion of neurite outgrowth from PC12 cells cultured on adsorbed trimeric L1 was increased by 100%, whereas on monomeric L1 the increase was only 50% over the control value. Promotion of neurite outgrowth from PC12 cells was specifically inhibited in a concentration-dependent manner by a polyclonal antibody against L1. These findings show that clustering of only three extracellular domains increases considerably L1's binding affinity to different ligands and enhances neurite outgrowth, suggesting that adhesive functions of L1 on the cell surface depend on cluster formation.
authors.affiliation: Department of Biophysical Chemistry, Biozentrum, University of Basel, Basel, Switzerland. hall@biomed.mat.ethz.ch
authors.forename: H
authors.initials: H
authors.lastname: Hall
authors.forename: D
authors.initials: D
authors.lastname: Bozic
authors.forename: C
authors.initials: C
authors.lastname: Fauser
authors.forename: J
authors.initials: J
authors.lastname: Engel
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D019556:COS Cells; D002942:Circular Dichroism; D015870:Gene Expression; D007797:Laminin / Q000378:metabolism; D039841:Leukocyte L1 Antigen Complex; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D010084:Oxidation-Reduction; D016716:PC12 Cells / Q000648:ultrastructure; D017433:Protein Structure, Secondary; D051381:Rats; D017469:Receptors, Fibronectin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence; D014162:Transfection
chemical_list: D007797:Laminin; D039841:Leukocyte L1 Antigen Complex; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D017469:Receptors, Fibronectin; C121346:laminin 1