Dendritic translocation of the rat ferritin H chain mRNA.
To elucidate the mechanism regulating the selective transport of mRNAs to synaptic sites, we compared the synaptosomal mRNAs with those from the forebrain using the differential display method. The ferritin H chain mRNA was found to be highly enriched in the synaptosomes. In situ hybridization for the ferritin H chain mRNA in the cultured dissociated neurons and in the hippocampal brain slices demonstrated its existence in the dendritic region. These data clearly indicate the selective translocation of the ferritin H chain mRNA into the dendrites and suggested the local expression of ferritin at the synapse.
Ishimoto
T
T
Department of Organic Materials, Osaka National Research Institute, Japan.
Fujimori
K
K
Kasai
M
M
Taguchi
T
T
D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D016548:Prosencephalon / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013569:Synapses / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
D012333:RNA, Messenger; D005293:Ferritins