Expression of neural properties in olfactory cytokeratin-positive basal cell line.
A cytokeratin (CK)(+), horizontal basal cell (HBC)-like cell line from murine olfactory epithelium, DBC1.2, was characterized. Histochemically, it is CK14(+), CK18(-), neuron-specific tubulin (NST)(+) and capable of binding Bandeiraea simplicifolia lectin I. RT-PCR studies revealed that it expresses mRNAs for EGF receptor, NST, Pax-6, but not for Mash-1 and neurogenin-1. Retinoic acid reduced the expression of CK14 and CKs recognized by polyclonal anti-keratin antibody, whereas it induced nestin. These results suggest that DBC1.2 shares the properties of HBCs and neural cells.
Satoh
M
M
Institute for Fermentation, Osaka (IFO), 2-17-85 Juso-honmachi, Yodogawa-ku, 532-8686, Osaka, Japan. satoh-motonobu@ifo.or.jp
Yoshida
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D051792:Basic Helix-Loop-Helix Proteins; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics; D005136:Eye Proteins; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018398:Homeodomain Proteins; D007633:Keratins / Q000032:analysis* / Q000235:genetics*; D037102:Lectins; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics; D019006:Neural Cell Adhesion Molecules / Q000032:analysis; D009831:Olfactory Mucosa / Q000737:chemistry* / Q000166:cytology*; D018034:Olfactory Receptor Neurons / Q000737:chemistry / Q000166:cytology; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D037121:Plant Lectins; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology; D014404:Tubulin / Q000032:analysis / Q000235:genetics; D014981:Xenopus
D000970:Antineoplastic Agents; D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D066246:ErbB Receptors; D005136:Eye Proteins; D018398:Homeodomain Proteins; D007633:Keratins; D037102:Lectins; D009419:Nerve Tissue Proteins; D019006:Neural Cell Adhesion Molecules; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D037121:Plant Lectins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D014212:Tretinoin; D014404:Tubulin; C499317:Ascl1 protein, mouse; C045475:Griffonia simplicifolia lectins; C000605732:Pax6 protein, mouse; C486336:Neurog1 protein, mouse