title: Immunohistochemistry can be used to subtype acute myeloid leukemia in routinely processed bone marrow biopsy specimens. Comparison with flow cytometry.
abstract: Flow cytometry (FC) is the preferred method of immunophenotyping acute myeloid leukemia (AML). However, there are situations in which FC is unavailable and in which immunohistologic staining of bone marrow biopsy specimens can be used to provide immunophenotypic information. To evaluate immunohistologic staining and to confirm its value, we selected 80 newly diagnosed cases of AML that were classified according to French-American-British (FAB) criteria and confirmed by flow cytometric analysis for this study. Paraffin-embedded bone marrow specimens were stained using a panel of antibodies that included CD34 (QBEND10), antimyeloperoxidase (anti-MPO), antihemoglobin, factor VIII-related antigen, and 3 epitopes of CD68 (HAM56, KP1, and PG-M1). Our findings suggest that with the use of the paraffin-reactive antibodies CD34 (QBEND10), MPO, CD68 (PG-M1), antihemoglobin, and factor VIII-related antigen, immunohistochemistry can be used to subclassify AML. Comparison of immunohistochemical results with FC immunophenotyping suggests that there is significant concordance in the results for markers that can be used with both techniques, indicating that the sensitivity and specificity of both methods is comparable (P > .53 in all cases).
authors.affiliation: Indiana University School of Medicine, Indianapolis, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Manaloor
authors.forename: R S
authors.initials: RS
authors.lastname: Neiman
authors.forename: D K
authors.initials: DK
authors.lastname: Heilman
authors.forename: M
authors.initials: M
authors.lastname: Albitar
authors.forename: T
authors.initials: T
authors.lastname: Casey
authors.forename: T
authors.initials: T
authors.lastname: Vattuone
authors.forename: P
authors.initials: P
authors.lastname: Kotylo
authors.forename: A
authors.initials: A
authors.lastname: Orazi
mesh_terms: D000208:Acute Disease; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis; D001706:Biopsy; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D002452:Cell Count; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007951:Leukemia, Myeloid / Q000145:classification* / Q000378:metabolism / Q000473:pathology; D016612:Paraffin Embedding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor