[Quality of whole blood as a result of storage and preparation (inline-leukocyte depletion). Evidence for autologous predeposit].
OBJECTIVE
Investigation of various laboratory parameters in stored whole blood, with respect to duration of storage and kind of product.


METHODS
Whole blood was donated by 12 healthy volunteers using CPDA1 stabilisator. Six units were filtered with the Leukotrap A1-System (PALL Comp., Dreieich, Germany) for leukocyte depletion. The twelve units were stored for 49 days. Several hematological, biochemical and coagulatory parameters were analysed during storage.


RESULTS
There was an adequate reduction of lycocytes by filtration (< 3 x 10(6) white cells per unit). ATP decreased during storage to 45% of initial value at the 49th day, without any influence of the kind of preparation. The course of other parameters such as lactate and free haemoglobin (increase), PH-value (decrease), antithrombin III (decrease), prothrombin, protein C, thrombin-antithrombin-complex, alpha-2-Anti-plasmin (decrease or indifferent) did not show any influence of the kind of preparation. Coagulation factors V and VIII decreased in both preparations, which was significantly less pronounced in whole blood with leukocyte depletion. In contrast parameters of activated coagulation such as D-Dimere and fibrinmonomeric did not change during storage after leukocyte reduction but increased at the end of storage time in CPDA1-blood.


CONCLUSIONS
Several parameters indicating quality of stored blood were constant in whole blood independent of the kind of preparation during a storage of 49 days. This is in contrast to the main part of specific scientific communications. A beneficial influence of leukocyte depletion was observed for some coagulation parameters whereas increasing characteristics of activated coagulation in CPDA1-stored whole blood at the end of storage time had to be observed. The preparation of whole CPDA1-blood is recommended for autologous predonation, if storage time does not exceed 30 days. Storage time of > 40 days seems to be possible for autologous whole blood after filtration for leucocyte depletion.
von Bormann
B
B
Klinik für Anaesthesiologie, operative Intensivmedizin und Schmerztherapie, St. Johannes-Hospital, Duisburg-Hamborn. vbormann@jodu.de
Wirtz
S
S
Weiler
J
J
von Bormann
C
C
Trobisch
H
H
D000255:Adenosine Triphosphate / Q000097:blood; D001771:Blood Banks; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D001777:Blood Coagulation; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion / Q000592:standards*; D001804:Blood Transfusion, Autologous; D006801:Humans; D007962:Leukocytes / Q000502:physiology*
D000255:Adenosine Triphosphate
Die Qualität von Vollblut in Abhängigkeit von Herstellungsverfahren (Leukozytendepletion) und Lagerungsdauer. Bedeutung für die operationsvorbereitende Eigenblutspende.