Effects of hydrogel/silver coatings on in vitro adhesion to catheters of bacteria associated with urinary tract infections.
Sections of sterile all-silicone-, hydrogel/silver-all-silicone-, and hydrogel/silver-latex-Foley urinary catheters were exposed to suspensions of bacteria and Candida albicans associated with urinary tract infections. The adhesion of these microorganisms to the catheters was determined with a radiolabel-cell procedure and scanning electron microscopy. Anomalous data with the radiolabel procedure were produced with the hydrogel/silver-latex catheters for certain species. These aberrant data were related to adhesion on the untreated cut ends of the latex catheter. Radiolabel-cell-adhesion procedures that involve sections of coated materials may need to be supplemented with additional procedures such as scanning electron microscopy for valid interpretations of the data. Adhesion to the hydrogel/silver catheters by both Gram-positive- and Gram-negative bacteria most commonly associated with nosocomial urinary tract infections, including a strain of Pseudomonas aeruginosa noted for its superior adhesion capacity, was significantly lower than the adhesion to the control all-silicone catheter.
Ahearn
D G
DG
Center for Applied and Environmental Biology, Georgia State University, Atlanta 30302-4010, USA.
Grace
D T
DT
Jennings
M J
MJ
Borazjani
R N
RN
Boles
K J
KJ
Rose
L J
LJ
Simmons
R B
RB
Ahanotu
E N
EN
D001422:Bacterial Adhesion; D002176:Candida albicans / Q000472:pathogenicity; D006090:Gram-Negative Bacteria / Q000302:isolation & purification / Q000472:pathogenicity; D006094:Gram-Positive Bacteria / Q000472:pathogenicity; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D066298:In Vitro Techniques; D007840:Latex; D008855:Microscopy, Electron, Scanning; D012828:Silicones; D012834:Silver; D013997:Time Factors; D014546:Urinary Catheterization / Q000009:adverse effects* / Q000295:instrumentation; D014552:Urinary Tract Infections / Q000382:microbiology* / Q000517:prevention & control
D007840:Latex; D012828:Silicones; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012834:Silver