title: A small-angle X-ray solution scattering study of bovine alpha-crystallin.
abstract: The native high molecular mass form of alpha-crystallin, the most important soluble protein in the eye lens, and its low molecular mass form obtained at 37 degrees C in dilute solutions were investigated by synchrotron radiation small-angle X-ray scattering. The alpha-crystallin solutions are polydisperse and good fits to the experimental data can be obtained using distributions of spheres with radii varying between about 5 and 10 nm. In spite of the polydispersity, two different ab initio methods were used to retrieve low resolution shapes from the scattering data. These shapes correspond to the z-average structure of the oligomers. In the absence of any symmetry constraints, the scattering curves of the two forms of alpha-crystallin yield bean-like shapes. The shape corresponding to the low molecular mass form has about 20% less mass at the periphery. Imposing tetrahedral symmetry on the average structures worsens the fit to the experimental data. We emphasized the apparent contradiction between hydrodynamic and molecular properties of alpha-crystallin. An explanation was put forward based on the presence of solvent-exposed flexible C-terminal extensions. We present two bead models ('hollow globule with tentacles' and 'bean with tentacles') based on NMR and cryo-electron microscopy studies and discuss how well they correspond with our data from X-ray scattering, light scattering and analytical ultracentrifugation.
authors.affiliation: Biophysics Research Group, Department of Biochemistry, University of Antwerp, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Vanhoudt
authors.forename: S
authors.initials: S
authors.lastname: Abgar
authors.forename: T
authors.initials: T
authors.lastname: Aerts
authors.forename: J
authors.initials: J
authors.lastname: Clauwaert
mesh_terms: D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000737:chemistry*; D008958:Models, Molecular; D012542:Scattering, Radiation; D012996:Solutions; D014965:X-Rays
chemical_list: D003459:Crystallins; D012996:Solutions