title: P2X receptor immunoreactivity in the male genital organs of the rat.
abstract: The distribution of ATP ionotropic P2X receptors in the genital organs of the male rat has been investigated with immunohistochemical techniques using specific antibodies to P2X1-7 receptors. In the excretory ducts of the testis (ductus epididymidis, vas deferens and its associated seminal vesicles), the major signals were seen with antibodies to P2X1 and P2X2 in the membranes of the smooth muscle layer, suggesting that these receptors are involved in the process of sperm transport and ejaculation. In the penis body, strong P2X1 and weaker P2X2 immunoreactivity was seen in the smooth muscle of blood vessels and the corpus cavernosum, suggesting a participation in the detumescence process. P2X5 immunoreactivity, a marker for differentiating cells in stratified squamous epithelia, was observed in the epithelia of the terminal urethra, the "horny spur" (spine-studded epithelium of the glans) and the inner surface of the prepuce. Antibodies to P2X3 reacted with nerve fibres in the adventitia of vas deferens, and the P2X6 receptor was localised in the basal lamina of the epithelium. In the prostate, there was immunostaining of the smooth muscle between the tubules with antibody for P2X1, but not with P2X2; P2X3 immunostaining of nerves and strong P2X7 immunostaining of the glandular epithelium of the prostate were also present.
authors.affiliation: Department of Anatomy, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Bardini
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001360:Avidin; D001710:Biotin; D004822:Epididymis / Q000378:metabolism / Q000648:ultrastructure; D005837:Genitalia, Male / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D009218:Myosins / Q000378:metabolism; D010413:Penis / Q000378:metabolism / Q000648:ultrastructure; D011467:Prostate / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000378:metabolism* / Q000648:ultrastructure; D012669:Seminal Vesicles / Q000378:metabolism / Q000648:ultrastructure; D014521:Urethra / Q000378:metabolism / Q000648:ultrastructure; D014649:Vas Deferens / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D007074:Immunoglobulin G; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D001360:Avidin; D001710:Biotin; D009218:Myosins