Simple detection method for distinguishing dead and living yeast colonies.
A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies.
Kucsera
J
J
Department of Ultrastructure and Molecular Function, Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan. kucsera@bio.u-szeged.hu
Yarita
K
K
Takeo
K
K
D001487:Basidiomycota / Q000166:cytology* / Q000187:drug effects; D002176:Candida albicans / Q000166:cytology* / Q000187:drug effects; D004396:Coloring Agents / Q000633:toxicity; D005840:Gentian Violet / Q000633:toxicity; D008751:Methylene Blue / Q000633:toxicity; D012395:Rose Bengal / Q000633:toxicity; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects; D012568:Schizosaccharomyces / Q000166:cytology* / Q000187:drug effects; D014343:Trypan Blue / Q000633:toxicity
D004396:Coloring Agents; D012395:Rose Bengal; D014343:Trypan Blue; D005840:Gentian Violet; D008751:Methylene Blue