Isolation and characterization of an Achromobacter xylosoxidans strain B3 and other bacteria capable to degrade the synthetic chelating agent iminodisuccinate.
Three bacterial strains were isolated, which used the synthetic chelating agent iminodisuccinate (IDS) as sole carbon source for growth in mineral salts media (MSM). Taxonomic analysis and 16S rDNA sequence analysis identified one of these isolates (B3), which was isolated from sewage sludge, as a strain of Achromobacter xylosoxidans subsp. xylosoxidans. It exhibited a doubling time of approximately 3 h in liquid MSM supplemented with IDS and grew even in the presence of 1.0% (w/v) IDS. Since photometric and high performance liquid chromatography analysis showed that IDS, which came onto the market only recently as an alternative for ethylenediaminetetraacetate, was completely degraded by axenic cultures of bacteria; it will probably be readily degraded in the environment.
Reinecke
F
F
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Germany.
Groth
T
T
Heise
K P
KP
Joentgen
W
W
Müller
N
N
Steinbüchel
A
A
D000421:Alcaligenes / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002614:Chelating Agents / Q000138:chemical synthesis / Q000378:metabolism*; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007098:Imino Acids / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D012988:Soil Microbiology; D013053:Spectrophotometry / Q000379:methods; D013386:Succinates / Q000737:chemistry / Q000378:metabolism*
D000596:Amino Acids; D002614:Chelating Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007098:Imino Acids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013386:Succinates; C412825:iminodisuccinic acid