Antigenic characterization of the nucleocapsid protein of Newcastle disease virus by means of a new panel of monoclonal antibodies.
Nine monoclonal antibodies (MAB) against nucleocapsid protein (NP) of Newcastle disease virus (NDV) have been prepared and characterized. All the MABs were classified into three groups by means of the competitive binding assay. At least three antigenic sites were delineated on the NP. The 1st site includes two closely located epitopes; the 2nd site includes two related and two distinct epitopes; the 3rd site includes two closely related and one distinct epitopes.
Panshin
A
A
Division of Avian Diseases, Kimron Veterinary Institute, Beit Dagan, Israel.
Shihmanter
E
E
Weisman
Y
Y
Orvell
C
C
Kydyrmanov
A
A
Asanov
N
N
Daulbaeva
K
K
Sayatov
M
M
Lipkind
M
M
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000145:classification / Q000276:immunology*; D001667:Binding, Competitive; D007132:Immunoglobulin Isotypes; D009522:Newcastle disease virus / Q000145:classification / Q000276:immunology*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000145:classification / Q000276:immunology*; D013045:Species Specificity; D014764:Viral Proteins / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007132:Immunoglobulin Isotypes; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014764:Viral Proteins; C409209:nucleoprotein, Newcastle disease virus