title: Selective vulnerability to radiation in the hippocampal dentate granule cells.
abstract: BACKGROUND
  Radiation therapy is an effective approach in the treatment of highly radiosensitive brain tumors such as germinomas. However, recent studies have reported intellectual disturbances in patients who underwent whole-brain irradiation as children. We detected apoptosis in the infantile murine cerebrum after systemic X-ray irradiation.
  
  
  METHODS
  Subjects were 100 ICR mice 4 weeks old, of which 90 were systemically exposed to 18 Gy X-rays (0.45 Gy/min); 10 each were decapitated and the cerebrums were removed 1, 3, 6, 9, 12, 18, 24, 48, and 72 hours after irradiation. Controls were 10 unirradiated mice. DNA fragmentation analysis was carried out by agarose gel electrophoresis, and morphological analysis was by the TUNEL method.
  
  
  RESULTS
  According to agarose gel electrophoresis, the cerebral DNA ladders were detected only over 6 to 24 hr, peaking in 9 hr. Even at the peak, band intensity was nearly double that of the unirradiated normal thymus. According to the TUNEL analysis, radiation-induced apoptosis increased, with a peak at 9 hours, but decreased 24 hours after irradiation. Apoptotic cells were always localized exclusively in the hippocampal dentate granule cells.
  
  
  CONCLUSIONS
  We found that vulnerability to radiation existed in the hippocampal dentate granule cells. Intellectual disturbances in patients who have undergone whole-brain irradiation may be caused by injury to the hippocampus.
authors.affiliation: Graduate School of Science and Laboratory of Radiation Biology, Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Nagai
authors.forename: S
authors.initials: S
authors.lastname: Tsunoda
authors.forename: Y
authors.initials: Y
authors.lastname: Hori
authors.forename: H
authors.initials: H
authors.lastname: Asada
mesh_terms: D000208:Acute Disease; D000818:Animals; D016371:Cranial Irradiation / Q000009:adverse effects*; D053938:DNA Fragmentation / Q000528:radiation effects; D018891:Dentate Gyrus / Q000473:pathology / Q000528:radiation effects*; D004587:Electrophoresis, Agar Gel; D051379:Mice; D008813:Mice, Inbred ICR; D011832:Radiation Injuries / Q000209:etiology*; D014916:Whole-Body Irradiation / Q000009:adverse effects*