title: Dendritic translocation of the rat ferritin H chain mRNA.
abstract: To elucidate the mechanism regulating the selective transport of mRNAs to synaptic sites, we compared the synaptosomal mRNAs with those from the forebrain using the differential display method. The ferritin H chain mRNA was found to be highly enriched in the synaptosomes. In situ hybridization for the ferritin H chain mRNA in the cultured dissociated neurons and in the hippocampal brain slices demonstrated its existence in the dendritic region. These data clearly indicate the selective translocation of the ferritin H chain mRNA into the dendrites and suggested the local expression of ferritin at the synapse.
authors.affiliation: Department of Organic Materials, Osaka National Research Institute, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishimoto
authors.forename: K
authors.initials: K
authors.lastname: Fujimori
authors.forename: M
authors.initials: M
authors.lastname: Kasai
authors.forename: T
authors.initials: T
authors.lastname: Taguchi
mesh_terms: D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D003712:Dendrites / Q000378:metabolism*; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D017403:In Situ Hybridization; D016548:Prosencephalon / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013569:Synapses / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D005293:Ferritins