A rapid measurement of apoptosis-associated light scatter changes using a hematology analyzer.
The alternative application of an automated hematology analyzer, H*3 system, has been described for the detection of apoptosis. Apoptosis induction by the topoisomerase I inhibitor, camptothecin (CAM) on several cell lines is followed by typical morphological alterations. On the H*3 cytogram, measurement of CAM-treated cells revealed an increased population of cells with reduced size suggesting cell contraction during apoptosis. The decreased LUC/Lymph ratio also indicated the enhanced degree of apoptosis directly correlated with increasing CAM concentration and/or incubation period. Quantitative analysis shows a good correlation between the H*3 measurement and flow cytometry measurements of Annexin V-fluorescein isothiocyanate-labeled method. Thus, the H*3 measurement, under an appropriate adjustment, can be used as a rapid monitor for evaluating the degree of apoptotic changes in drug susceptibility testing of homogeneous cell samples.
Lertworasirikul
T
T
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Bunyaratvej
A
A
D017209:Apoptosis / Q000187:drug effects; D002166:Camptothecin / Q000494:pharmacology; D002452:Cell Count; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D059004:Topoisomerase I Inhibitors; D014407:Tumor Cells, Cultured
D004791:Enzyme Inhibitors; D059004:Topoisomerase I Inhibitors; D002166:Camptothecin