Biosynthesis of avenacins and phytosterols in roots of Avena sativa cv. Image.
In keeping with the proposal that avenacin biosynthesis is restricted to the tips of primary roots of oat seedlings, the incorporation of radioactivity from R-[2-(14)C]mevalonic acid (MVA) into avenacins and beta-amyrin by serial sections of primary roots was found to be more-or-less restricted to root tip sections. Squalene synthase (SQS) (EC 2.5.1.21) and 2,3-oxidosqualene:beta-amyrin cyclase (OS beta AC) (EC 5.4.99) were also most active in these sections. The incorporation of radiolabel from R-[2-(14)C]MVA into cycloartenol and 24-methylene cycloartanol by, and the 2,3-oxidosqualene:cycloartenol cyclase (OSCC) (EC 5.4.99) activity in, the various serial sections were consistent with phytosterol biosynthesis occurring in all the sections of the root with some tailing-off in the rate of synthesis in the more distal sections.
Trojanowska
M R
MR
Department of Biological Sciences, University of Hull, UK. miranda.trojanowska@bbsrc.ac.uk
Osbourn
A E
AE
Daniels
M J
MJ
Threlfall
D R
DR
D018554:Avena / Q000201:enzymology / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000378:metabolism; D019751:Intramolecular Transferases / Q000378:metabolism; D008798:Mevalonic Acid / Q000378:metabolism; D010840:Phytosterols / Q000096:biosynthesis*; D018517:Plant Roots / Q000201:enzymology / Q000378:metabolism; D012503:Saponins / Q000096:biosynthesis*
D002250:Carbon Radioisotopes; D010840:Phytosterols; D012503:Saponins; C091460:avenacin A 1; D013186:Farnesyl-Diphosphate Farnesyltransferase; D019751:Intramolecular Transferases; C039924:2,3-oxidosqualene-beta-amyrin-cyclase; D008798:Mevalonic Acid