Molecular basis for differing antineurogenic effects of GATA-1a and GATA-1b in Xenopus.
The erythroid transcription factor GATA-1 in Xenopus has been cloned as a pair of presumably duplicated genes designated as xGATA-1a and xGATA-1b. Although both xGATA-1a and xGATA-1b are able to stimulate erythropoiesis, only xGATA-1b is capable of inhibiting neurogenesis in Xenopus embryos. Chimeras of these two genes were constructed by permuting coding and untranslated regions (UTR) on both ends of these two xGATA-1, and their neurogenesis-inhibitory effects were studied. These results reveal that (1) sequence variations between the coding regions alone do not account for the neurogenesis effect; (2) 3' UTR of xGATA-1a causes the loss of the neurogenesis inhibition of xGATA-1b; (3) 3' UTR of xGATA-1b is essential to inhibit neurogenesis. In addition, the presence of either UTR does not affect the stability of the mRNA in vitro. These observations suggest the influence of 3' UTR in xGATA-1 on the inhibition of neurogenesis.
Chen
H D
HD
Endocrinology and Reproduction Research Branch, NICHD, Bethesda, Maryland 20892, USA. hdchen@helix.nih.gov
Huang
Y K
YK
Ault
K
K
Wong
G W
GW
Lin
M C
MC
Chen
H C
HC
Kung
H F
HF
D020413:3' Untranslated Regions; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D002678:Chimera; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D050981:Erythroid-Specific DNA-Binding Factors; D020440:Gene Duplication; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009154:Mutation; D009420:Nervous System / Q000196:embryology*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D014982:Xenopus laevis
D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D014157:Transcription Factors; D004247:DNA