Effect of dietary lipid (soybean lecithin and triacylglycerol) on hepatic F-actin microfilaments in cyclosporine A-treated rats: image analysis by confocal laser scanning microscopy.
We studied and quantified the effect of cyclosporine A on hepatic F-actin on bile canalicular and basolateral membranes in rats fed either soybean lecithin, triacylglycerol-enriched diet, or low-fat diet by means of confocal laser scanning microscopy imaging. The phalloidin-FITC staining of F-actin was quite normal in the lecithin-cyclosporine A group but decreased significantly in the other cyclosporine A-treated groups (by 40% and 25% of control in triacylglycerol-cyclosporine A and cyclosporine A groups, respectively). The alteration of F-actin by cyclosporine A, related to cholestasis evidenced by a decrease in bile salt secretion, was prevented by dietary soybean lecithin and amplified by dietary soybean triacylglycerol.
Benkoel
L
L
Laboratoire de Pathologie Cellulaire et Métabolique du Foie, INSERM U260, Faculté de Médecine, Marseille, France.
Chanussot
F
F
Dodero
F
F
De la Maisonneuve
C
C
Bongrand
P
P
Benoliel
A M
AM
Lambert
R
R
Brisse
J
J
Chamlian
A
A
D000199:Actins / Q000378:metabolism / Q000502:physiology*; D000546:Amanitins; D000818:Animals; D001646:Bile / Q000378:metabolism; D016572:Cyclosporine / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D018613:Microscopy, Confocal; D010713:Phosphatidylcholines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max; D014280:Triglycerides / Q000494:pharmacology*
D000199:Actins; D000546:Amanitins; D004041:Dietary Fats; D010713:Phosphatidylcholines; D014280:Triglycerides; C018358:phallotoxin; D016572:Cyclosporine