Distribution of P2X purinoceptor clusters on individual rat dorsal root ganglion cells.
The distribution of P2X receptors on the cell bodies of acutely dissociated rat dorsal root ganglion cells has been determined using immunohistochemistry. Only medium and small ganglion cells showed P2X receptor labelling, which took the form of receptor clusters 0. 2-0.5 microm in diameter. P2X(3) was the predominant cluster type although clusters of all other subtypes, P2X(1), P2X(2), P2X(4), P2X(5) and P2X(6) were observed. There was little evidence for colocalization of the different receptor subtypes pointing to very little heteropolymerization of P2X receptors in the ganglion.
Barden
J A
JA
Protein Receptor Structure Laboratory, Department of Anatomy and Histology, University of Sydney, Australia.
Bennett
M R
MR
D000818:Animals; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000737:chemistry / Q000166:cytology*; D007150:Immunohistochemistry; D009475:Neurons, Afferent / Q000737:chemistry*; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000032:analysis*; D058469:Receptors, Purinergic P2X; D058476:Receptors, Purinergic P2X2; D058477:Receptors, Purinergic P2X3; D058485:Receptors, Purinergic P2X5
C546403:P2rx2 protein, rat; C546410:P2rx3 protein, rat; C546467:P2rx5 protein, rat; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D058476:Receptors, Purinergic P2X2; D058477:Receptors, Purinergic P2X3; D058485:Receptors, Purinergic P2X5; C110590:purinoceptor P2X6