[Functional characteristics of the promoter region of the tag7/PGRP gene in KSML0, KSML100 murine mammary adenocarcinoma cell ines and VMR liver].
A study was carried out on the transcription regulation of the tag7/PGRP gene, whose product is similar to cytokines and is involved in recognizing peptidoglycane in mouse mammary adenocarcinoma cell lines KSML0, KSML100, and VMR-liver. The 3250-bp fragment of the promoter region was sequenced and tested for DNase I-hypersensitive sites (DHSs). In the KSML0 cells line, DHSs were found in the vicinity of the TATA box and approximately at position -3000 relative to the transcription start of the gene. Only a DHS cluster near the TATA box was found in the VMR-liver cell line. Regions involved in transcription regulation of the gene in the three cell lines were identified with the use of reporter constructions carrying the CAT gene under the control of deletion derivatives of the tag7/PGRP promoter region. The minimal promoter including only the TATA box with the nearest neighboring sequences was inactive in all cell lines. The elements (enhancers) positively regulating gene transcription in KSML0 and VMR-liver were mapped to region -192, -48. An element accounting for the transcriptional inactivity of the gene in KSML100 cells was assigned to region -315, -3250.
Ruzov
A S
AS
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. egorbp@mx.ibg.rssi.ru
Georgiev
G P
GP
Prokhorchuk
E B
EB
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D016207:Cytokines / Q000235:genetics*; D017931:DNA Primers; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D051379:Mice; D011401:Promoter Regions, Genetic; D016385:TATA Box; D014158:Transcription, Genetic
D002352:Carrier Proteins; D016207:Cytokines; D017931:DNA Primers; C113582:Pglyrp1 protein, mouse; C118897:peptidoglycan recognition protein; D015500:Chloramphenicol O-Acetyltransferase
Funktsional'naia kharakteristika promotornoĭ oblassti gena tag7/PGRP v kletkakh liniĭ KSML0, KSML100 i VMR-pechen' adenokartsinom molochnoĭ zhelezy myshi.