Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation.
Clostridium beijerinckii BA101 (mutant strain) and C. beijerinckii 8052 (wild type) were compared for substrate and butanol inhibition. The wild-type strain is more strongly inhibited by added butanol than is the mutant strain. Acetone and butanol were removed from a fed-batch reactor inoculated with C. beijerinckii BA101 by pervaporation using a silicone membrane. In the batch reactor, C. beijerinckii BA101 produced 25.3 g/L of total solvents, whereas in the fermentation-recovery experiment it produced 165.1 g/L of total solvents. Solvent productivity increased from 0.35 (batch reactor) to 0.98 g/L.h (fed-batch reactor). The fed-batch reactor was fed with 500 g/L of glucose-based P2 medium. Acetone selectivities ranged from 2 to 10 whereas butanol selectivities ranged from 7 to 19. Total flux varied from 26 to 31 g/m2.h.
Qureshi
N
N
University of Illinois, Department of Food Science and Human Nutrition, Urbana 61801, USA. nqureshi@uiuc.edu
Blaschek
H P
HP
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D000440:Butanols / Q000302:isolation & purification / Q000378:metabolism; D003013:Clostridium / Q000235:genetics / Q000502:physiology*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008567:Membranes, Artificial; D012828:Silicones; D012997:Solvents; D013171:Spores, Bacterial / Q000378:metabolism
D000440:Butanols; D008567:Membranes, Artificial; D012828:Silicones; D012997:Solvents; D005947:Glucose