Mucosal immunization with experimental feline immunodeficiency virus (FIV) vaccines induces both antibody and T cell responses but does not protect against rectal FIV challenge.
Feline immunodeficiency virus (FIV) is a natural lentiviral pathogen of cats which can be experimentally transmitted via rectal and vaginal routes--the major routes of human immunodeficiency virus type 1 transmission in man. An important objective for lentiviral research is the development of vaccine strategies which generate good mucosal immune responses capable of giving protection from a mucosal virus challenge. The experimental vaccines employed in this study were based on (a) a peptide from the third variable region of the FIV envelope glycoprotein and (b) fixed whole FIV, Glasgow-8 strain. Adjuvants used were Quil A and cholera toxin for mucosal administration and incomplete Freund's adjuvant and immune stimulating complexes for subcutaneous injection. Mucosal immunization was given by rectal and intranasal routes. Both antibody and proliferative responses were elicited by mucosal immunization and cholera toxin was found to be a good mucosal adjuvant. The addition of a lipo thioester to the FIV peptide improved IgG and IgA responses upon parenteral administration. However, no protection from a rectal FIV challenge was achieved.
Finerty
S
S
Department of Clinical Veterinary Science, University of Bristol, Langford, BS40 5DU, Bristol, UK. sue.finerty@bristol.ac.uk
Stokes
C R
CR
Gruffydd-Jones
T J
TJ
Hillman
T J
TJ
Reeves
N A
NA
Whiting
C V
CV
Schaaper
W M
WM
Dalsgaard
K
K
Harbour
D A
DA
D000281:Administration, Intranasal; D000285:Administration, Rectal; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002415:Cats; D007114:Immunization; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D012007:Rectum / Q000821:virology*; D013601:T-Lymphocytes / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014765:Viral Vaccines