A study on the nitric oxide production-suppressing activity of sanguisorbae radix components.
The active components of an aqueous extract of Sanguisorbae Radix, which possesses nitric oxide (NO) production-suppressing activity, were determined using macrophages that were activated by the addition of lipopolysaccharide. Significant inhibitory activity against the formation of both NO and inducible NO synthase, and NADPH-diaphorase activity, which is involved in NO generation, was shown by Sanguisorbae Radix fractions T-B and T-C. On further fractionation, the subfractions of T-B and T-C all showed high anti-NO activity. Sanguiin H-6, sanguiin H-11, 1,2,3,4,6-penta-O-galloyl-beta-D-glucose, eugeniin and polymeric proanthocyanidin were isolated from TB-3 and TC-4, and all were identified as exhibiting strong anti-NO activity. We have confirmed that sanguiin H-6 is the most active component of Sanguisorbae Radix with respect to the suppression of NO production. It is suggested that tannin makes a prominent contribution to the biological activity of Sanguisorbae Radix.
Yokozawa
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Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan. yokozawa@ms.toyama-mpu.ac.jp
Chen
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CP
Tanaka
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Kitani
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D000818:Animals; D000872:Anthocyanins / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology; D047348:Hydrolyzable Tannins; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D044945:Proanthocyanidins; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013634:Tannins / Q000737:chemistry / Q000494:pharmacology
D000872:Anthocyanins; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D047348:Hydrolyzable Tannins; D044945:Proanthocyanidins; D013634:Tannins; C406326:sanguiin H 11; C013221:proanthocyanidin; D009569:Nitric Oxide; C110634:eugeniin; D005707:Gallic Acid; C081844:sanguiin H 6; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D009252:NADPH Dehydrogenase