99Tcm-tetrofosmin: evaluation of fractionated cold kits and two new methods of quality control.
The fractionation of Myoview into 1:5 was followed by storage at -80 degrees C for 3 months. The kits were reconstituted with 3.5 GBq 99Tcm-pertechnetate and the average radiochemical purity (RCP) was maintained at 96.5 +/- 1.6% (n = 24) for 24 h. There was no difference in biodistribution between the 1:5 fraction and the full kit, especially with respect to myocardial uptake. These observations show that it is possible to fractionate and store Myoview kits at -80 degrees C and produce a product that is stable over 3 months. This provides a cost-effective method for using 99Tcm-tetrofosmin. Two new methods of determining RCP were also examined and found to give results that were not significantly different from the recommended manufacturer's method. A mini paper chromatography (MPC) method using Whatman 17 paper and a solvent mixture of ethyl acetate: acetone (1:9) and an Amprep SAX cartridge method using absolute ethanol as the solvent were found to be more rapid and reliable methods of quality control for this product.
Penglis
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S
Royal Adelaide Hospital Radiopharmacy, Department of Nuclear Medicine, Australia. s.penglis@unisa.edu.au
Tsopelas
C
C
D000818:Animals; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D015609:Organotechnetium Compounds / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D011786:Quality Control; D011854:Radiochemistry; D015203:Reproducibility of Results; D014018:Tissue Distribution
D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; C078700:technetium tc-99m tetrofosmin