title: [Isolation and characteristics of low molecular weight recombinant peptides, representing antigenic domains in the NP protein of lymphocytic choriomeningitis virus].
abstract: High- and low-molecular recombinant peptides of LCM virus nucleoprotein, representing individual immunodominant antigenic sites, were obtained in pJC40 expressing vector and studied in solid-phase enzyme immunoassay. 2-7% proteins resultant from total cellular synthesis are recombinant peptides. Comparative analysis of antigenic properties of recombinant peptides and native viral protein showed that recombinant peptides are virtually not inferior to native viral protein in antigenic properties.
authors.forename: A S
authors.initials: AS
authors.lastname: Vladyko
authors.forename: I K
authors.initials: IK
authors.lastname: Fomin
authors.forename: E P
authors.initials: EP
authors.lastname: Scheslenok
mesh_terms: D000956:Antigens, Viral / Q000737:chemistry*; D001483:Base Sequence; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D007124:Immunoenzyme Techniques; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D008970:Molecular Weight; D009698:Nucleoproteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D017931:DNA Primers; D009698:Nucleoproteins; D010446:Peptide Fragments; D011994:Recombinant Proteins
vernacular_title: Poluchenie i kharakteristika nizkomolekuliarnykh rekombinantnykh peptidov, predstavliaiushchikh antigennye domeny belka NP virusa limfotsitarnogo khoriomeningita.