title: Processing of frameshifted vasopressin precursors.
abstract: Biosynthesis of the vasopressin (VP) prohormone in magnocellular neurones of the hypothalamo-neurohypophysial system comprises endoplasmic reticulum (ER) transit, sorting into the regulated secretory pathway and subsequent processing in the individual proteins VP, neurophysin and a glycoprotein. These processes are severely disrupted in the homozygous diabetes insipidus (di/di) Brattleboro rat, which expresses a mutant VP precursor due to a single nucleotide deletion in the neurophysin region of the VP gene resulting in VP deficiency. Previous studies have shown the presence of additional frameshift mutations in VP transcripts, in solitary magnocellular neurones of the di/di rat due to a GA dinucleotide deletion resulting in two different mutant VP precursors with partly restored reading frame. Frameshifted VP precursors are also expressed in several magnocellular neurones in wild-type rats. In this study, we determined if the +1 frameshifted precursors from di/di and wild-type rats can lead to biosynthesis of the hormone VP. Therefore, eukaryotic expression plasmids containing the frameshifted VP cDNAs were transiently expressed in peptidergic tumour cell lines, and cells were analysed by reversed phase high-performance liquid chromatography and specific radioimmunoassays, and by immunofluoresence. Neuro2A neuroblastoma cells expressing the +1 frameshifted precursors of di/di rats retained products in the cell body. Only precursor or insignificant quantities of neurophysin-immunoreactive products were detected. In contrast, in AtT20 cells, frameshifted VP precursors were at least partly processed to yield the VP peptide, indicating that they have access to the regulated secretory pathway. Comparison between the two cell lines showed a very slow ER transit of the wild-type prohormone combined with inefficient processing in Neuro2A cells. The results show that mutant precursors can reach the regulated secretory pathway if ER transport is sufficiently rapid as in the case of AtT20 cells. This suggests that the di/di rat may regain the capacity to biosynthesize authentic VP through these +1 frameshifted precursors in magnocellular neurones.
authors.affiliation: Rudolf Magnus Institute for Neurosciences, Department of Medical Pharmacology, Utrecht University, Universiteitsweg, Utrecht, The Netherlands.
authors.forename: D A
authors.initials: DA
authors.lastname: Evans
authors.forename: F M
authors.initials: FM
authors.lastname: De Bree
authors.forename: M
authors.initials: M
authors.lastname: Nijenhuis
authors.forename: A A
authors.initials: AA
authors.lastname: Van Der Kleij
authors.forename: R
authors.initials: R
authors.lastname: Zalm
authors.forename: N
authors.initials: N
authors.lastname: Korteweg
authors.forename: F W
authors.initials: FW
authors.lastname: Van Leeuwen
authors.forename: J P
authors.initials: JP
authors.lastname: Burbach
mesh_terms: D000818:Animals; D001692:Biological Transport; D003919:Diabetes Insipidus / Q000235:genetics; D004721:Endoplasmic Reticulum / Q000378:metabolism; D016368:Frameshift Mutation; D017353:Gene Deletion; D051379:Mice; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D051381:Rats; D011910:Rats, Brattleboro; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
chemical_list: D011498:Protein Precursors; D014667:Vasopressins