Evaluation of a Yersinia adhesion gene (yadA) specific PCR for the identification of enteropathogenic Yersinia enterocolitica.
A total of 101 Yersinia enterocolitica strains was investigated with a PCR assay [Blais and Phillipe, Food Control, 6 (1995) 211-214] targeting the Yersinia adhesin gene (yadA) responsible for autoagglutination. Compared to the autoagglutination test the PCR assay has a specificity of 100% but a sensitivity of only 70%. This failure might be caused by the sequence heterogeneity of yadA.
Neubauer
H
H
Institute of Microbiology, German Armed Forces Medical Academy, Munich. 320027945810-0001@t-online.de
Hensel
A
A
Aleksic
S
S
Meyer
H
H
D018829:Adhesins, Bacterial / Q000235:genetics*; D000372:Agglutination Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D015008:Yersinia enterocolitica / Q000302:isolation & purification* / Q000472:pathogenicity
D018829:Adhesins, Bacterial; C064832:YadA protein, Yersinia