Genetic diversity of Ralstonia solanacearum as assessed by PCR-RFLP of the hrp gene region, AFLP and 16S rRNA sequence analysis, and identification of an African subdivision.
The genetic diversity among strains in a worldwide collection of Ralstonia solanacearum, causal agent of bacterial wilt, was assessed by using three different molecular methods. PCR-RFLP analysis of the hrp gene region was extended from previous studies to include additional strains and showed that five amplicons were produced not only with all R. solanacearum strains but also with strains of the closely related bacteria Pseudomonas syzygii and the blood disease bacterium (BDB). However, the three bacterial taxa could be discriminated by specific restriction profiles. The PCR-RFLP clustering, which agreed with the biovar classification and the geographical origin of strains, was confirmed by AFLP. Moreover, AFLP permitted very fine discrimination between different isolates and was able to differentiate strains that were not distinguishable by PCR-RFLP. AFLP and PCR-RFLP analyses confirmed the results of previous investigations which split the species into two divisions, but revealed a further subdivision. This observation was further supported by 16S rRNA sequence data, which grouped biovar 1 strains originating from the southern part of Africa.
Poussier
Stéphane
S
Laboratoire de Phytopathologie, CIRAD-FLHOR, 97448 Saint-Pierre Cedex, La Réunion, France1.
Trigalet-Demery
Danielle
D
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP27, 31326 Castanet-Tolosan Cedex, France2.
Vandewalle
Peggy
P
Laboratoire de Phytopathologie, CIRAD-FLHOR, 97448 Saint-Pierre Cedex, La Réunion, France1.
Goffinet
Bruno
B
Unité de Biométrie et d'Intelligence Artificielle, INRA, BP27, 31326 Castanet-Tolosan Cedex, France3.
Luisetti
Jacques
J
Laboratoire de Phytopathologie, CIRAD-FLHOR, 97448 Saint-Pierre Cedex, La Réunion, France1.
Trigalet
André
A
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA-CNRS, BP27, 31326 Castanet-Tolosan Cedex, France2.
D000349:Africa; D001426:Bacterial Proteins / Q000235:genetics*; D020118:Cupriavidus necator / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D013045:Species Specificity; D014157:Transcription Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C118343:PrhJ protein, Ralstonia solanacearum; D012336:RNA, Ribosomal, 16S; D014157:Transcription Factors