Distribution of bile acid absorption and bile acid transporter gene message in the hamster ileum.
The apical, Na-dependent, ileal bile acid transporter (IBAT) is critical for the reabsorption of bile acids in the ileum. Bile acid transport capacities as well as the distribution of bile acid transporter messenger ribonucleic acid (mRNA) and transporter protein were studied along the axis of the ileum. Na-dependent and Na-independent taurocholate uptake was measured in the hamster ileum using an everted-sleeve technique. The distribution of IBAT mRNA and protein were mapped by in-situ hybridization, immunohistochemistry, and Western blotting. Na-dependent and Na-independent bile acid uptake rates were highest 1-4 cm before the ileocecal valve (maxima 780 and 120 pmol/mm2 per min, respectively) and decreased proximally and distally. Na-independent absorption was increased in the last 6 cm of the ileum. IBAT mRNA and protein expression were linked closely to the distribution of uptake capacity. IBAT mRNA was more abundant near the crypt-villus junction whereas the protein was expressed evenly along the villus axis. We conclude that Na-dependent and Na-independent bile acid absorption capacities both have distinct distribution curves in the hamster ileum. All ileocytes on villi in the high-uptake area of the ileum express IBAT mRNA and protein.
Stelzner
M
M
Department of Surgery, University of Washington, Seattle 98108, USA. stelzner@u.washington.edu
Hoagland
V
V
Somasundaram
S
S
D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D006224:Cricetinae; D003720:Densitometry; D007082:Ileum / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D027981:Symporters; D013656:Taurocholic Acid / Q000378:metabolism / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D002352:Carrier Proteins; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger; D027981:Symporters; C071908:sodium-bile acid cotransporter; D013656:Taurocholic Acid; D012964:Sodium