Purge-and-trap gas chromatographic determination of styrene in urine and blood. Application to exposed workers.
A simple purge-and-trap gas chromatographic method with flame ionization detection was developed for the determination of styrene in urine and blood. Styrene present in a 5 ml sample at room temperature was swept by helium at 40 ml/min for 11 min, trapped on a Tenax trap, desorbed by heating, cryofocused, and injected by flash heating into a DB-5 capillary GC column. The oven temperature program was from 80 degrees C, held for 8 min, to 120 degrees C at 5 degrees C/min, and then held for 2 min. The detector temperature was 250 degrees C. The calibration curves were linear in the range of 2.5-15 ppb styrene in urine and 25-150 ppb in blood. The detection limits calculated were 0.4 microg/l in urine and 0.6 microg/l in blood. The coefficients of variations within the day and day-to-day were 3 and 3.1%, respectively, for 2.5 ppb of styrene in urine, and 1 and 1.6% for 25 ppb of styrene in blood. The results obtained from samples taken from workers exposed to styrene were reported.
Prieto
M J
MJ
División de Medicina Legal y Forense, Facultad de Medicina, Universidad Miguel Hernández, San Juan, Alicante, Spain.
Berenguer
V
V
Marhuenda
D
D
Cardona
A
A
D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D008297:Male; D016273:Occupational Exposure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020058:Styrene / Q000032:analysis* / Q000097:blood / Q000652:urine
D020058:Styrene