Cloning and expression of a new site-specific methyltransferase M.SscL1I from Staphylococcus sp. L1.
The gene of the new site-specific methyltransferase M.SscL1I belonging to the same modification-restriction system as the previously described by us site-specific endonuclease SscL1I has been cloned from the natural strain Staphylococcus sp. L1. A plasmid to express the methylase gene under control of the T7 phage-specific promotor has been constructed. Conditions were found to express the recombinant methylase M.SscL1I and to purify it to near homogeneity. It is shown that the methylase modifies the adenine base in the recognition site 5;-GANTC-3;.
Vasiljeva
L Y
LY
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
Zheleznaya
L A
LA
Matvienko
N I
NI
D002850:Chromatography, Gel; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013210:Staphylococcus / Q000201:enzymology*; D013379:Substrate Specificity
D011994:Recombinant Proteins; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases