hsp70, hsp32, and grp78 are increased in thermally injured skin with and without antithrombin(human) concentrate infusion.
An acquired deficiency of antithrombin (AT), an anti-inflammatory protein, develops in patients with thermal injuries. Skin thermotolerance is regulated by heat shock protein (hsp) genes. hsp70, hsp32, hsp27, and glucose-regulated protein78 (grp78) were studied in burned and unburned human skin to determine whether correction of the AT deficiency modulated the intensity of expression of these proteins. Fifty-four human skin samples were prepared by Western blot analysis: 11 unburned and 22 burned control skin samples and 7 unburned and 14 burned skin samples from patients treated with AT(Human), or AT(H). The intensity of hsp32 expression in burned AT(H)-treated skin (P < .001) and in burned control skin (P < .01) was significantly increased compared with unburned control skin. The intensity of expression of hsp70 was statistically significant in burned AT(H)-treated skin compared with unburned control skin (P < .02), as was that of grp78 (P < .01). Thermally injured skin with or without AT(H) treatment had an increased expression of hsp70, hsp32, and grp78 compared with unburned control skin.
Kowal-Vern
A
A
Department of Pathology, Loyola University Medical Center, Maywood, Ill 60153, USA.
Goral
J
J
Gamelli
R L
RL
McGill
V
V
Clancy
J
J
D000293:Adolescent; D000328:Adult; D000990:Antithrombin III / Q000494:pharmacology / Q000627:therapeutic use*; D020152:Antithrombin III Deficiency / Q000209:etiology / Q000503:physiopathology; D015153:Blotting, Western; D002056:Burns / Q000150:complications / Q000188:drug therapy / Q000503:physiopathology*; D002352:Carrier Proteins / Q000096:biosynthesis*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones / Q000096:biosynthesis*
D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D000990:Antithrombin III