A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 1: direct binding.
The nuclear import receptor karyopherin alpha recognizes nuclear localization signals (NLSs), peptides that direct the transport of proteins into the nucleus. A simple, colorimetric assay has been developed to facilitate the identification and comparison of karyopherin ligands by direct and competitive binding using NLSs immobilized on the solid phase (TentaGel resin).
Connolly
M D
MD
Department of Chemistry, Texas A&M University, College Station 77842-3012, USA.
Park
S B
SB
Reedy
B M
BM
Standaert
R F
RF
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors; D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry; D001667:Binding, Competitive / Q000187:drug effects; D001710:Biotin / Q000737:chemistry; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003124:Colorimetry; D007202:Indicators and Reagents; D007211:Indoles / Q000737:chemistry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects / Q000378:metabolism*; D019809:Streptavidin; D028901:alpha Karyopherins
D000952:Antigens, Polyomavirus Transforming; D007202:Indicators and Reagents; D007211:Indoles; D009687:Nuclear Proteins; D010455:Peptides; D018160:Receptors, Cytoplasmic and Nuclear; D028901:alpha Karyopherins; C035455:5-bromo-4-chloro-3-indoxyl phosphate; D001710:Biotin; D019809:Streptavidin; D000469:Alkaline Phosphatase