Efficiency of erythropoietin's signal peptide for HIV(MN)-1 gp 120 expression.
The HIV-1 gp120 gene with natural signal sequence expressed in eukaryotic expression systems showed extremely low levels of synthesis and secretion. Several expression systems have been used to improve the secretion levels of gp 120. In mammalian cells, the efficient expression of gp120 fused to t-PA signal peptide has been previously reported. Here, the effects of t-PA and EPO signal peptides were compared as secretion sequences for expression of gp120 in COS-7 cells. The EPO's signal peptide is used for the first time as leader sequence for secretion of foreign proteins. Our results indicated that higher amounts of secreted gp 120 were obtained when vectors containing EPO signal peptide were used.
Herrera
A M
AM
Division of Vaccines, Centro de Ingenieria Genética y Biotecnologia, Cdad. Habana, 10600, Cuba. amherrera@cigb.edu.cu
Musacchio
A
A
Fernández
J R
JR
Duarte
C A
CA
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D004921:Erythropoietin / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D015699:HIV Envelope Protein gp120; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D004921:Erythropoietin; D010959:Tissue Plasminogen Activator