Non-electrostatic complexes with DNA: towards novel synthetic gene delivery systems.
We have developed new DNA complexing amphiphile based on Hoechst 33258 interaction with DNA grooves. The synthesis and physicochemical characterisation of lipid/DNA complexes, as compared to that of classical lipopolyamine for gene delivery, are described and discussed.
Soto
J
J
UMR-7001 ENSCP/CNRS/Aventis Pharma Gencell, Vitry sur Seine, France.
Bessodes
M
M
Pitard
B
B
Mailhe
P
P
Scherman
D
D
Byk
G
G
D006690:Bisbenzimidazole / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium / Q000737:chemistry; D005456:Fluorescent Dyes; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006860:Hydrogen Bonding; D008055:Lipids; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D005456:Fluorescent Dyes; D008055:Lipids; D000644:Quaternary Ammonium Compounds; D004247:DNA; D004996:Ethidium; D006690:Bisbenzimidazole