Development of 16S rDNA-based PCR assay for detecting centipeda periodontii and selenomonas sputigena.
To detect oral motile bacteria directly from dental plaque, specific PCR primers for Centipeda periodontii and Selenomonas sputigena were designed based on the sequence analysis of their 16S rDNA. The primers were specific and sensitive enough to amplify DNA fragments from the available oral bacteria. The detection limit was fewer than 10 bacterial cells per sample. It was also possible to detect these bacteria in dental plaque. The prevalence of these bacteria varied in each sample. The specific primers designed in this study may clarify the epidemiology of periodontal disease.
Sawada
S
S
Department of Periodontology and Endodontology, Okayama University Dental School, Okayama, Japan.
Kokeguchi
S
S
Takashiba
S
S
Murayama
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D005881:Gingiva / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020357:Selenomonas / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S