title: Elevation of anti-cytokeratin 18 antibody and circulating cytokeratin 18: anti-cytokeratin 18 antibody immune complexes in sera of patients with idiopathic pulmonary fibrosis.
abstract: In this study, we hypothesize that anti-cytokeratin 18 (CK18) antibody and CK18:anti-CK18 immune complex increase in sera in patients with idiopathic pulmonary fibrosis (IPF). To prove the existence of anti-CK18 antibodies in patients' sera, bovine CK18 was stained with patients' sera using a Western blotting. In patients with IPF, anti-CK18 antibodies were clearly demonstrated in sera by Western blotting. Then, we tried to establish an enzyme-linked immunosorbent assay (ELISA) to quantify anti-CK18 antibodies and CK18:anti-CK18 immune complexes in sera of patients with IPF. Levels of anti-human CK18 antibodies in sera of patients with IPF (0.81 +/- 0.31, mean +/- SD) measured by ELISA were significantly high compared with that of normal volunteers (0.45 +/- 0.06, p < 0.01). In addition, levels of CK18:anti-CK18 antibody complexes in patients' sera (0.64 +/- 0.35, man +/- SD) significantly increased compared with those of normal subjects (0.40 +/- 0.10, p < 0.01). These results suggest that anti-CK18 antibody and its immune complex may have played a role in the process of lung injury in IPF.
authors.affiliation: First Department of Internal Medicine, Kagawa Medical University, Kagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Dobashi
authors.forename: J
authors.initials: J
authors.lastname: Fujita
authors.forename: M
authors.initials: M
authors.lastname: Murota
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: I
authors.initials: I
authors.lastname: Yamadori
authors.forename: T
authors.initials: T
authors.lastname: Yoshinouchi
authors.forename: R
authors.initials: R
authors.lastname: Ueda
authors.forename: S
authors.initials: S
authors.lastname: Bandoh
authors.forename: T
authors.initials: T
authors.lastname: Kamei
authors.forename: M
authors.initials: M
authors.lastname: Nishioka
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: J
authors.initials: J
authors.lastname: Takahara
mesh_terms: D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000097:blood* / Q000276:immunology; D001323:Autoantibodies; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D002455:Cell Division / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007633:Keratins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011650:Pulmonary Alveoli / Q000276:immunology / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000097:blood* / Q000276:immunology / Q000473:pathology; D012189:Retrospective Studies; D012720:Severity of Illness Index
chemical_list: D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D015415:Biomarkers; D007633:Keratins