title: Nitric oxide binding to nitrophorin 4 induces complete distal pocket burial.
abstract: The nitrophorins comprise an unusual family of proteins that use ferric (Fe(III)) heme to transport highly reactive nitric oxide (NO) from the salivary gland of a blood sucking bug to the victim, resulting in vasodilation and reduced blood coagulation. We have determined structures of nitrophorin 4 in complexes with H2O, cyanide and nitric oxide. These structures reveal a remarkable feature: the nitrophorins have a broadly open distal pocket in the absence of NO, but upon NO binding, three or more water molecules are expelled and two loops fold into the distal pocket, resulting in the packing of hydrophobic groups around the NO molecule and increased distortion of the heme. In this way, the protein apparently forms a 'hydrophobic trap' for the NO molecule. The structures are very accurate, ranging between 1.6 and 1.4 A resolutions.
authors.affiliation: Department of Biochemistry, University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: A
authors.initials: A
authors.lastname: Weichsel
authors.forename: J F
authors.initials: JF
authors.lastname: Andersen
authors.forename: S A
authors.initials: SA
authors.lastname: Roberts
authors.forename: W R
authors.initials: WR
authors.lastname: Montfort
mesh_terms: D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D003486:Cyanides / Q000378:metabolism; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006420:Hemeproteins / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D012239:Rhodnius / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry* / Q000378:metabolism*; D014867:Water / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D003486:Cyanides; D006420:Hemeproteins; D012471:Salivary Proteins and Peptides; C090560:nitrophorin; D014867:Water; D009569:Nitric Oxide; D006418:Heme