Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein.
Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.
Blance
S J
SJ
Department of Biochemistry, University of Leicester, United Kingdom.
Williams
N L
NL
Preston
Z A
ZA
Bishara
J
J
Smyth
M S
MS
Maxwell
A
A
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D003374:Coumarins / Q000737:chemistry; D018360:Crystallography, X-Ray; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II / Q000737:chemistry* / Q000235:genetics; D019008:Drug Resistance, Neoplasm; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005998:Glycine / Q000737:chemistry; D008956:Models, Chemical; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009675:Novobiocin / Q000031:analogs & derivatives / Q000494:pharmacology; D010641:Phenotype; D011487:Protein Conformation; D013489:Suppression, Genetic; D013696:Temperature; D013816:Thermodynamics; D014633:Valine / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D003374:Coumarins; D004791:Enzyme Inhibitors; D009675:Novobiocin; C006260:clorobiocin; C030123:coumarin; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II; D014633:Valine; D005998:Glycine