title: Floating cultivation of marine cyanobacteria using coal fly ash.
abstract: The aim of this study was to develop improved methodologies for bulk culturing of biotechnologically useful marine cyanobacteria in the open ocean. We have investigated the viability of using coal fly ash (CFA) blocks as the support medium in a novel floating culture system for marine micro-algae. The marine cyanobacterium Synechococcus sp. NKBG 040607 was found to adhere to floating CFA blocks in liquid culture medium. Maximum density of attached cells of 2.0 x 10(8) cells/cm2 was achieved using seawater. The marine cyanobacterium Synechococcus sp. NKBG 042902 weakly adhered to floating CFA blocks in BG-11 medium. Increasing the concentration of calcium ion in the culture medium enhanced adherence to CFA blocks.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
authors.forename: E
authors.initials: E
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Takeyama
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D001709:Biotechnology / Q000379:methods; D002118:Calcium / Q000494:pharmacology; D002130:Calcium Phosphates / Q000494:pharmacology; D002244:Carbon; D003031:Coal; D060729:Coal Ash; D003470:Culture Media; D000458:Cyanobacteria / Q000187:drug effects / Q000254:growth & development*; D052638:Particulate Matter; D012623:Seawater / Q000382:microbiology*
chemical_list: D002130:Calcium Phosphates; D003031:Coal; D060729:Coal Ash; D003470:Culture Media; D052638:Particulate Matter; D002244:Carbon; D002118:Calcium