title: Chromatin texture analysis of cortical adrenal gland adenomas, including incidentalomas, and adjacent normal-appearing cortical tissue.
abstract: OBJECTIVE
  To describe, by morphometric and chromatin texture analysis, a series of adrenal gland lesions, including Cushing's and Conn's adenomas and incidentalomas.
  
  
  METHODS
  The material for the study consisted of five consecutive cases of incidentaloma, three cases of Conn's adenoma and three cases of Cushing's adenoma. Also included were five cases of adrenal carcinoma. Sections were stained according to the Feulgen procedure. Measurements were taken from the nodules and from two different zones, identified as outer and inner parts, of the normal-appearing adrenal cortex adjacent to the tumor. Data on approximately 50 nuclei were recorded for each of these three sites (tumor and outer and inner normal-appearing adrenal cortex). The nuclei were subjected to feature extraction and were analyzed by identification procedures--i.e., establishing nuclear and lesion signatures.
  
  
  RESULTS
  The total optical density (OD) distributions of the nuclei from the normal-appearing adrenal cortex pointed to their diploid or near-diploid nature. In incidentalomas there was a very small increase in the number of nuclei, with increased total OD. In Conn's adenoma there was a noticeable but modest extension of the total OD distribution into the higher OD range. This trend continued for Cushing's adenoma. The pixel OD histograms for nuclei from normal-appearing tissue and from incidentalomas were hardly distinguishable. Starting with nuclei from Conn's adenoma, a shift toward lower pixel OD values began. The trend continued for nuclei from Cushing's adenoma and was very pronounced for nuclei from carcinoma. The nuclear signatures showed no appreciable difference between nuclei from normal-appearing cortex and from incidentaloma. Nuclei from Conn's adenoma were more similar to those from normal tissue in their signatures than nuclei from Cushing's adenoma. In fact, the nuclear signatures from Cushing's adenoma were almost identical to those of carcinoma. The lesion signatures for normal tissue, incidentaloma and Conn's adenoma confirmed the results seen in the nuclear signatures. There was a very modest increase in the number of nuclei with greater deviation from normal in incidentalomas, and the trend was more obvious in Conn's adenoma. However, in Cushing's adenoma there was a very substantial increase in the number of nuclei, with large deviations of their nuclear chromatin texture from normal.
  
  
  CONCLUSIONS
  Computer-assisted analysis of nuclear characteristics proved useful in identifying and describing differences between groups of tumors arising in the adrenal cortex and highlighted the similarity between incidentalomas and adjacent normal-appearing cortex and between Cushing's adenoma and adrenal carcinoma.
authors.affiliation: Institute of Pathology, University of Ancona, Italy. m.scarpelli@popcsi.unian.it
authors.forename: M
authors.initials: M
authors.lastname: Scarpelli
authors.forename: M G
authors.initials: MG
authors.lastname: Baccarini
authors.forename: P
authors.initials: P
authors.lastname: Colanzi
authors.forename: G
authors.initials: G
authors.lastname: Arnaldi
authors.forename: R
authors.initials: R
authors.lastname: Montironi
authors.forename: D
authors.initials: D
authors.lastname: Thompson
authors.forename: P H
authors.initials: PH
authors.lastname: Bartels
mesh_terms: D000236:Adenoma / Q000145:classification / Q000473:pathology*; D000293:Adolescent; D000306:Adrenal Cortex Neoplasms / Q000145:classification / Q000473:pathology*; D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000145:classification / Q000473:pathology*; D002843:Chromatin / Q000473:pathology*; D003480:Cushing Syndrome / Q000473:pathology; D005260:Female; D006801:Humans; D006929:Hyperaldosteronism / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged
chemical_list: D002843:Chromatin