Exploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized beds.
Two polysaccharides, alginate and chitosan, showed unusual affinity and bound alpha-amylase (from various sources) and Aspergillus niger cellulase, respectively. The beads prepared from these polymers were successfully used for the purification of the respective enzymes by fluidized bed affinity chromatography. alpha-amylase from wheat germ could be purified by 58-fold with about 90% recovery of activity. Aspergillus niger cellulase, on the other hand, was purified by 30-fold with 80% recovery of enzyme activity. Both purified preparations show single band on SDS-PAGE.
Roy
I
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, 110016, New Delhi, India
Sardar
M
Gupta
MN