An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains.
To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.
van Dijken JP
Department of Microbiology and Enzymology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
Bauer
J
Brambilla
L
Duboc
P
Francois
JM
Gancedo
C
Giuseppin
ML
Heijnen
JJ
Hoare
M
Lange
HC
Madden
EA
Niederberger
P
Nielsen
J
Parrou
JL
Petit
T
Porro
D
Reuss
M
van Riel N
Rizzi
M
Steensma
HY
Verrips
CT
Vindeløv
J
Pronk
JT