title: Correlation between IL-8 induction, cagA status and vacA genotypes in 153 French Helicobacter pylori isolates.
abstract: The polymorphism of clinical presentations associated with Helicobacter pylori infection is potentially due to differences in the virulence of individual strains. H. pylori virulence has been associated with the ability to induce secretion of interleukin-8 (IL-8), the vacA genotypes, and the cagA status. The aim of this study was to determine the virulence profiles of 153 French H. pylori isolates on the basis of vacA genotypes, cagA status, and IL-8 induction ability. A total of 153 H. pylori isolates from patients with chronic gastritis (n = 74) or gastro-duodenal ulcers (n = 79) was examined for vacA genotypes and cagA status by polymerase chain reaction (PCR) and dot blot, and for their ability to induce IL-8 secretion by HEp-2 cells. The prevalence of vacA genotypes was: s1/m1 44.3%, s1/m2 24.9%, and s2/m2 23.5%. The cagA gene was present in 64% of the strains. IL-8 secretion was induced by 58.7% of the isolates. The presence of the cagA gene was significantly correlated with the s1/m1 vacA genotype and with the induction of IL-8. Thirty-four strains were atypical (cagA-positive/IL-8 noninducer or cagA-negative/IL-8 inducer). vacA genotypes, cagA status, and IL-8 induction ability are not correlated with the presence or absence of ulcer. The cagA status is not sufficient to predict the proinflammatory ability of H. pylori.
authors.affiliation: Laboratoire de microbiologie A, IFR-FR59 (CNRS), CHU La Milétrie, Poitiers, France. celine_audibert@hotmail.com
authors.forename: C
authors.initials: C
authors.lastname: Audibert
authors.forename: B
authors.initials: B
authors.lastname: Janvier
authors.forename: B
authors.initials: B
authors.lastname: Grignon
authors.forename: L
authors.initials: L
authors.lastname: Salaün
authors.forename: C
authors.initials: C
authors.lastname: Burucoa
authors.forename: J C
authors.initials: JC
authors.lastname: Lecron
authors.forename: J L
authors.initials: JL
authors.lastname: Fauchère
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D005260:Female; D005756:Gastritis / Q000382:microbiology; D005798:Genes, Bacterial; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D006801:Humans; D015151:Immunoblotting; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016209:Interleukin-8; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori