Quantification of the influence of single-point mutations on haloalkane dehalogenase activity: a molecular quantum similarity study.
Controlled modifications in certain protein amino acid residues can lead to changes in their function and stability. Amino acid structural features and their relation to these changes were examined by using quantum molecular similarity techniques. The effect of deliberate mutations in position 172 of the haloalkane dehalogenase enzyme, yielding to variations on the dehalogenation of 1,2-dibromoethane, was studied qualitatively and quantitatively using molecular quantum similarity techniques. A valuable classification of the residues according to their effect on activity was obtained by representing the optimal two-dimensional classical scaling solution. In addition, satisfactory quantitative relationships were found, comparable to those attained by previous studies on this same data set using other techniques. Molecular quantum similarity analysis provides a consistent, unbiased, and homogeneous set of molecular descriptors and is a feasible alternative to the use of physicochemical properties.
Robert
D
D
Institute of Computational Chemistry, University of Girona, Catalonia, Spain.
Gironés
X
X
Carbó-Dorca
R
R
D006867:Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D011789:Quantum Theory; D013329:Structure-Activity Relationship
D006867:Hydrolases; C046137:haloalkane dehalogenase