title: A flow injection chemiluminescence method using Cr(III) as a catalyst for determining hydrogen peroxide. Application to H(2)O(2) determination in cultures of microalgae.
abstract: Flow injection analysis has been applied to the determination of hydrogen peroxide produced by some different species of microalgae. The method is based on the luminol-H(2)O(2) chemiluminescence reaction using Cr(III) as a catalyst. Optimum experimental conditions for the method have been studied and trace amounts of hydrogen peroxide determined with detection limits of 4 10(-8) mol/L. The method using Cr(III) was compared with that using horseradish peroxidase as the catalyst.
authors.affiliation: Department of Analytical Chemistry, University of Seville, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Escobar
authors.forename: S
authors.initials: S
authors.lastname: García-Domínguez
authors.forename: A
authors.initials: A
authors.lastname: Guiraúm
authors.forename: O
authors.initials: O
authors.lastname: Montes
authors.forename: F
authors.initials: F
authors.lastname: Galván
authors.forename: F F
authors.initials: FF
authors.lastname: de La Rosa
mesh_terms: D002384:Catalysis; D002857:Chromium; D004492:Edetic Acid; D056890:Eukaryota / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D017022:Flow Injection Analysis / Q000379:methods*; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol; D010777:Photochemistry
chemical_list: D002857:Chromium; D008165:Luminol; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase