Molecular identification and characterization of novel members of the human organic anion transporter (OATP) family.
We identified three novel transporters structurally belonging to the organic anion transporting polypeptide (OATP) family in humans. Since previously known rat oatp1 to 3 do not necessarily correspond to the human OATPs in terms of either tissue distribution or function, here we designate the newly identified human OATPs as OATP-B, -D and -E, and we rename the previously known human OATP as OATP-A. OATP-C proved to be identical with the recently reported LST1/OATP-2. Expression profiles of the five OATPs and the prostaglandin transporter PGT (a member of OATP family) in human tissues showed that OATP-C is exclusively localized in liver, OATP-A and PGT are expressed in restricted ranges of tissues, and OATP-B, -D and -E show broad expression profiles. OATP-B, -C, -D and -E exhibited transport activity for [(3)H]estrone-3-sulfate as a common substrate. OATP-C has a high transport activity with broad substrate specificity.
Tamai
I
I
Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, 920-0934, Japan.
Nezu
J
J
Uchino
H
H
Sai
Y
Y
Oku
A
A
Shimane
M
M
Tsuji
A
A
D000595:Amino Acid Sequence; D027321:Anion Transport Proteins; D017920:Antiporters / Q000737:chemistry / Q000235:genetics; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D015232:Dinoprostone / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism; D004970:Estrone / Q000031:analogs & derivatives / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009928:Organ Specificity; D027361:Organic Anion Transporters; D010400:Penicillin G / Q000378:metabolism; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D013379:Substrate Specificity; D014162:Transfection
D027321:Anion Transport Proteins; D017920:Antiporters; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D027361:Organic Anion Transporters; D012333:RNA, Messenger; C503526:SLCO2A1 protein, human; C503528:Slco2a1 protein, rat; C025483:estradiol-17 beta-glucuronide; D004970:Estrone; D004958:Estradiol; D015232:Dinoprostone; D010400:Penicillin G; C017296:estrone sulfate