Modulation of human interferon-gamma biosynthesis by antisense oligodeoxynucleotides.
We investigated the inhibition of human interferon-gamma (HuIFN-gamma) production in cultures of lymphocytes with the use of the antisense strategy. Out of a series of antisense oligodeoxynucleotides (ODN) complementary to different regions of the HuIFN-gamma gene, a 16-mer specific for a sequence including the translation initiation codon was the most effective. Here we describe a detailed protocol for the isolation of lymphocytes from buffy coats, the rational design of antisense ODN, and the monitoring of HuIFN-gamma production of the antisense ODN-treated cells.
Boeve
C M
CM
Laboratory for Biochemistry, Katholieke Universiteit Leuven, Belgium.
De Ley
M
M
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*
D016376:Oligonucleotides, Antisense; D007371:Interferon-gamma