title: Carboxyfluorescein fluorescence experiments.
abstract: Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern-Volmer representation for a dynamic quenching.
authors.affiliation: IPBS du CNRS, 205 Route de Narbonne 31077, Toulouse Cedex, France
authors.initials: S
authors.lastname: Massou
authors.initials: R
authors.lastname: Albigot
authors.initials: M
authors.lastname: Prats