cDNA cloning of bradykinin-potentiating peptides-C-type natriuretic peptide precursor, and characterization of the novel peptide Leu3-blomhotin from the venom of Agkistrodon blomhoffi.
A cDNA clone, 1.8 kb long, was isolated from a venom gland cDNA library of Agkistrodon blomhoffi that encodes a large plurifunctional precursor composed of 263 amino-acid residues. Nucleotide sequence analysis of this clone revealed that sequences which code for blomhotin and a novel peptide Leu3-blomhotin are located in the N-terminal region of the precursor polypeptide, followed by four tandemly aligned sequences which code for three types of bradykinin-potentiating peptide. In the C-terminal region, the sequence for the C-type natriuretic peptide was located along with a preceding processing signal. The deduced amino-acid sequences for the four bradykinin-potentiating peptides coincided exactly with previously known sequences for potentiator B, potentiator C and potentiator E. The actual Leu3-blomhotin peptide was subsequently isolated from the venom of A. blomhoffi and characterized. Leu3-blomhotin possesses contractile activity in isolated rat stomach fundus smooth muscle in the same manner as blomhotin. Furthermore, it was shown that blomhotin and Leu3-blomhotin retained activity to inhibit the angiotensin-converting enzyme.
Murayama
N
N
School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Michel
G H
GH
Yanoshita
R
R
Samejima
Y
Y
Saguchi
K
K
Ohi
H
H
Fujita
Y
Y
Higuchi
S
S
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000737:chemistry*; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type / Q000737:chemistry / Q000235:genetics*; D010455:Peptides / Q000302:isolation & purification*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D011817:Rabbits
D000806:Angiotensin-Converting Enzyme Inhibitors; D003435:Crotalid Venoms; D018076:DNA, Complementary; C411855:Leu3 blomhotin; D010455:Peptides; D011498:Protein Precursors; D020098:Natriuretic Peptide, C-Type