Reversible and oxidative inactivation of endogenous survival factors after removal of exogenous survival factors.
Serum contains exogenous survival factors for fibroblasts. Removal of these exogenous factors causes inactivation of endogenous survival factors, subsequent release of a constitutively expressed apoptosis machinery from negative control and apoptosis. Inactivation of endogenous survival factors after serum withdrawal is mediated by reactive oxygen species and can be rapidly reversed through re-addition of serum. Oxidative inactivation of endogenous survival factors after depletion of exogenous survival factors as well as the process of reversion to the active state do not require protein synthesis, indicating the existence of a constitutively expressed regulatory system that controls the interaction of exogenous and endogenous survival factors.
Engelmann
I
I
Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Freiburg, Germany.
Bauer
G
G
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001769:Blood; D002460:Cell Line; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology*; D003470:Culture Media; D016895:Culture Media, Serum-Free; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D007700:Kinetics; D051379:Mice; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D003470:Culture Media; D016895:Culture Media, Serum-Free; D011500:Protein Synthesis Inhibitors; D017382:Reactive Oxygen Species; D016212:Transforming Growth Factor beta; D003513:Cycloheximide