[The determination of lipopolysaccharide admixtures in protein preparations].
A new method for the determination of lipopolysaccharide (LPS) admixtures in protein solutions has been developed. The method includes the periodate oxidation of LPS, biotinylation with biotin hydraside, immobilization on a nitrocellulose membrane and the development of biotinylated LPS in the streptavidin--alkaline phosphatase system. Proteins are previously removed from the solution by treatment with hot phenol. Development with the use of 5-bromoinodyl phosphate and nitrotetrazolium blue makes it possible to detect about 30 pg of LPS immobilized on the nitrocellulose membrane.
Denisova
L Ia
LIa
Research Institute for Design and Technology of Biologically Active Substances, State Scientific Center of Virology and Biotechnology Vector, Berdsk, Novosibirsk Region, Russia.
Baturina
I I
II
Zakabunin
A I
AI
Afinogenova
G N
GN
Pustoshilova
N M
NM
D019921:Biotinylation; D002138:Calibration; D003101:Collodion; D004340:Drug Contamination; D004926:Escherichia coli; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000032:analysis*; D008567:Membranes, Artificial; D010504:Periodic Acid; D011994:Recombinant Proteins / Q000032:analysis*; D012996:Solutions
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D012996:Solutions; D010504:Periodic Acid; D003101:Collodion; C009288:metaperiodate
Opredelenie primeseĭ lipopolisakharidov v preparatakh belkov.