title: Motility is required to initiate host cell invasion by Yersinia enterocolitica.
abstract: Invasin-mediated invasion of host cells by the pathogen Yersinia enterocolitica was shown to be affected by flagellar-dependent motility. Motility appears to be required to ensure the bacterium migrates to and contacts the host cell. Nonmotile strains of Y. enterocolitica were less invasive than motile strains, but the reduction in invasion could be overcome by artificially bringing the bacteria into host cell contact by centrifugation. Mutations in known regulatory genes of the flagellar regulon, flhDC and fliA, resulted in less inv expression but did not have a significant effect on invasin levels. However, invasin levels were reduced for strains that harbored flhDC on a multicopy plasmid, apparently as a result of increased proteolysis of invasin.
authors.affiliation: Department of Molecular Microbiology, Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital, St. Louis, Missouri 63110, USA.
authors.forename: G M
authors.initials: GM
authors.lastname: Young
authors.forename: J L
authors.initials: JL
authors.lastname: Badger
authors.forename: V L
authors.initials: VL
authors.lastname: Miller
mesh_terms: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D029968:Escherichia coli Proteins; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D006801:Humans; D009068:Movement; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D018085:Regulon; D012808:Sigma Factor / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D015008:Yersinia enterocolitica / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C064971:FliA protein, Bacteria; D012808:Sigma Factor; D015534:Trans-Activators; C090552:flhC protein, E coli; C090553:flhD protein, E coli; C053476:invasin, Yersinia