title: Radioprotective effects of the thiols GSH and WR-2721 against X-ray-induction of micronuclei in erythroblasts.
abstract: The frequency of micronucleated polychromatic erythrocytes (MNPCEs) was assessed in the bone marrow and peripheral blood of adult male Swiss mice treated with reduced glutathione (GSH) and S-2-/3-aminopropylamino/ethyl phosphorothioic acid (WR-2721), at a dose of 400 mg/kg body weight, and exposed to 6 Gy X-rays. GSH or WR-2721 was applied alone, or 60 and 30 min, respectively, prior to X-ray-exposure. The number of MNPCEs was determined at 24 h after the thiol treatment and X-irradiation. The radioprotection and toxicity caused in the mouse erythroblasts by GSH and WR-2721, as indicated by the number of MNPCEs were dependent on the thiol applied. The stronger radioprotective effect is obtained following WR-2721 administration than after GSH application. WR-2721 showed greater toxicity than GSH. The combination of GSH and WR-2721 given before X-ray-exposure resulted in the most radioprotective effect as compared to the respective single-drug treatment of mice. Application of the both thiols, without subsequent X-irradiation appeared to be the most toxic, compared with administration of WR-2721 or GSH alone. The effective radioprotection by the combined action of GSH and WR-2721 against genomic instability induced in the mouse erythroblasts by X-rays was shown.
authors.affiliation: Department of Animal Physiology, Jagiellonian University, Cracow, Poland. maz@zuk.iz.uj.edu.pl
authors.forename: L
authors.initials: L
authors.lastname: Mazur
mesh_terms: D004999:Amifostine / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004357:Drug Synergism; D004900:Erythroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects* / Q000528:radiation effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014965:X-Rays
chemical_list: D011837:Radiation-Protective Agents; D005978:Glutathione; D004999:Amifostine