title: Expression of chemokines and their receptors in nephrotoxic serum nephritis.
abstract: BACKGROUND
  Chemokines play a major role in leukocyte infiltration in inflammatory kidney diseases. The specificity of the chemokine action is determined by the restricted expression of the corresponding receptors on leukocytes. We therefore simultaneously studied the expression of CC-chemokine and CC-chemokine receptor 1-5 (CCR 1-5) mRNA in an accelerated model of nephrotoxic nephritis in CD-1 mice.
  
  
  METHODS
  Kidneys were harvested at day 0, 2 and 7. Induction of nephritis was confirmed by assessment of albuminuria by ELISA and by histological evaluation. RNA was prepared from cortex and isolated glomeruli. RNase protection assays were performed to study the expression of chemokines, RNase protection assays as well as quantitative RT-PCR assays to study the expression of chemokine receptors.
  
  
  RESULTS
  In the cortex of nephritic kidneys mRNA for MCP-1 was increased 5-fold on day 2 and increased 4-fold on day 7 as compared to controls. mRNA for RANTES was increased 5-fold on day 7 and mRNA for IP-10 6-fold on day 7. The increase of mRNA for the chemokine receptors CCR1 and 5 was between 2-fold and 3-fold determined by RNase protection assay and for CCR1, 2 and 5 between 2- and 4-fold as determined by RT-PCR. In isolated glomeruli we found by RT-PCR an increase of CCR1, CCR2 and CCR5 of between 3 and 12-fold.
  
  
  CONCLUSIONS
  These results show that chemokines and their specific chemokine receptors are increased in parallel in this model of glomerulonephritis, consistent with the potential role of the chemokine system in leukocyte recruitment to the immune injured kidney.
authors.affiliation: Medizinische Poliklinik, Klinikum Innenstadt der Ludwigs-Maximilians-Universität, Munich, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Schadde
authors.forename: M
authors.initials: M
authors.lastname: Kretzler
authors.forename: B
authors.initials: B
authors.lastname: Banas
authors.forename: B
authors.initials: B
authors.lastname: Luckow
authors.forename: K
authors.initials: K
authors.lastname: Assmann
authors.forename: D
authors.initials: D
authors.lastname: Schlöndorff
mesh_terms: D000818:Animals; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007668:Kidney / Q000378:metabolism; D051379:Mice; D009393:Nephritis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D018925:Chemokines; D007106:Immune Sera; D020033:Protein Isoforms; D012333:RNA, Messenger; D019707:Receptors, Chemokine