Condition-dependent presence of beta-lipotropin-like peptide in human keratinocytes.
This study aimed to characterize the beta-endorphin-immunoreactive material (betaE-IR) detectable in normal human keratinocytes (NHK). The effects of different culturing conditions and UV-irradiation on production of betaE-IR by NHK were assessed by radioimmunoassay and HPLC. All culture systems contained low levels of betaE-IR that was increased in conditioned media after UV-irradiation under certain conditions. NHK grown in nutrient-poor medium contained highest levels of betaE-IR that exhibited beta-lipotropin-like properties after HPLC analysis. The other culturing conditions displayed no authentic betaE-related peptides. Our results indicate that under certain culturing conditions NHK can produce POMC peptides like beta-lipotropin, which can be induced by UV-radiation.
Wintzen
M
M
Department of Dermatology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands. mwintzen@knmg.nl
Zanello
S B
SB
Holick
M F
MF
Wiegant
V M
VM
Burbach
J P
JP
Vermeer
B J
BJ
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects; D011863:Radioimmunoassay; D014466:Ultraviolet Rays; D008083:beta-Lipotropin / Q000378:metabolism*
D008083:beta-Lipotropin