Microdialysis sampling coupled to HPLC for transdermal delivery study of ondansetron hydrochloride in rats.
The transdermal delivery of ondansetron hydrochloride (ON) solution in propylene glycol (PG) with a widely used penetration enhancer, oleic acid (OA), was studied in rats by a microdialysis sampling technique. Dialysate samples collected from the probe were directly injected into the HPLC system without any pre-treatment and no interference occurred in the blank sample. A good linearity between the standard concentrations and peak areas within the calibration range was achieved. In vivo recovery (32.52 +/- 1.8%) of the probe was assessed with the retrodialysis method, which was used to calculate the ON concentration in the dermis. Oleic acid at the concentrations of 2% and 5% (w/v) increased the steady-state delivery rate from 0.001 to 0.030 and 0.058 microg/h, respectively. OA proved to be an effective enhancer for transdermal delivery of ON in rats.
Ding
P
P
Department of Pharmacy, Shenyang Pharmaceutical University, People's Republic of China. dingpt402@yahoo.com
Xu
H
H
Wei
G
G
Zheng
J
J
D000279:Administration, Cutaneous; D000818:Animals; D000932:Antiemetics / Q000008:administration & dosage* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007700:Kinetics; D008297:Male; D017551:Microdialysis; D019301:Oleic Acid; D017294:Ondansetron / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D019946:Propylene Glycol; D051381:Rats; D012702:Serotonin Antagonists / Q000008:administration & dosage* / Q000032:analysis; D012867:Skin / Q000737:chemistry / Q000378:metabolism; D012996:Solutions
D000932:Antiemetics; D012702:Serotonin Antagonists; D012996:Solutions; D019301:Oleic Acid; D017294:Ondansetron; D019946:Propylene Glycol