Use of polymerase chain reaction to detect Brucella abortus biovar 1 in infected goats.
The polymerase chain reaction (PCR) was used to diagnose goat brucellosis and compare its sensitivity against some of the most commonly used serological and bacteriological techniques. Twenty two female and one male out of 300 clinically healthy, mixed-breed goats were randomly chosen from a ranch located at Marín, Nuevo León, Mexico. Milk and blood samples were taken from each animal and used to obtain both microbiological cultures and DNA of the pathogen, and sera was tested against Rose Bengal antigen (RBT). Results showed that 86% of the blood samples were positive on the PCR test, while 60% were positive on the serological test. The pathogen was isolated from only one blood culture. Sixty four percent of the milk samples were positive on PCR tests, but failed to yield bacteria in culture. Biochemical and PCR specific assay demonstrated that Brucella abortus biovar 1 was associated with the infection. This study demonstrates the higher sensitivity of PCR over RBT and blood culture and its potential towards a rapid identification of Brucella strains.
Leal-Klevezas
D S
DS
Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Col. Independencia, 44340, Jal. , Guadalajara, Mexico.
Martínez-Vázquez
I O
IO
García-Cantú
J
J
López-Merino
A
A
Martínez-Soriano
J P
JP
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D002003:Brucella abortus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats; D008297:Male; D008892:Milk / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012395:Rose Bengal / Q000737:chemistry; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D012395:Rose Bengal