[Mutation analysis of the beta-catenin gene in epithelial carcinomas of the ovaries].
beta-catenin is a continuously expressed cytoplasmic protein that has an important role is both E-cadherin-mediated cell-cell adhesion and in activation of Wnt/Wingless transcriptional pathway. The accumulation of stabilized beta-catenin caused by the mutation of the exon 3 of beta-catenin gene can stimulate the T-cell factor/Lymphoid enhancing factor-mediated transcriptional activation. The activation of transcriptional pathway may through oncogenes is an important step of the oncogenesis in solid tumors. In this study we analyzed mutations in exon 3 of the beta-catenin gene in 18 sporadic epithelial ovarian tumors. Three mutations were found from these 18 ovarian tumor samples which contained 8 serous, 3 mucinous, 5 endometrioid, one malignant Brenner-type tumor and one transitional cell carcinoma. Two mutations occurred in endometrioid-type (in 47 and 55 codons) and one in serous-type (in 47 codon) ovarian carcinomas, and both mutations were missense and somatic. The patients with mutated beta-catenin gene appeared from the younger patients under the age of 50. Our results suggest that the stabilization of beta-catenin protein by the mutation of CTNNB1 gene can contribute to the multistep process of the oncogenesis of epithelial ovarian tumors. Furthermore these mutations mostly occurs in the endometrioid-type of EOT, but can appear in other types such as serous-type ovarian tumor.
Tanyi
J
J
Altalános Orvostudományi Kar, Semmelweis Egyetem, Budapest.
Páy
A
A
Rigó
J
J
Nagy
B
B
Papp
Z
Z
D000328:Adult; D000367:Age Factors; D000368:Aged; D015820:Cadherins / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005973:Glutamine / Q000235:genetics; D006801:Humans; D007532:Isoleucine / Q000235:genetics; D008239:Lysine / Q000235:genetics; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012694:Serine / Q000235:genetics; D015534:Trans-Activators; D051176:beta Catenin
C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm; D015534:Trans-Activators; D051176:beta Catenin; D007532:Isoleucine; D005973:Glutamine; D012694:Serine; D008239:Lysine
A beta-catenin génmutáció analízise epithelialis ovarium-carcinomákban.