Interactions of invasive and noninvasive strains of Neisseria meningitidis with monkey epithelial cells, mouse monocytes and human macrophages.
Adherence and phagocytosis of invasive and noninvasive Neisseria meningitidis strains was investigated using light, fluorescence and electron microscopy. Invasive strains were isolated from the cerebrospinal fluid and/or blood of the patients with invasive meningococcal disease and noninvasive strains from the nasopharynx and/or larynx of healthy carriers. Adherence/endocytosis was studied on monkey kidney cells (the LLC-MK2 cell line) and phagocytosis on mouse monocytes and human macrophages (the P388D1 and U-937 cell lines, respectively). Although invasive and noninvasive meningococci isolated in the same cluster showed identical genotype and phenotype markers, they were found to interact differently with epithelial cells as well as with monocytes/macrophages. Invasive isolates displayed higher adherence to the surface of LLC-MK2 cells compared to noninvasive ones. Phagocytosis by P388D1 cells of noninvasive strains was effective and the bacteria were damaged by cytolysis. In contrast, invasive bacteria frequently persisted in "coiling" vacuoles and in effect could destroy the host cell. This is the first demonstration of coiling phagocytosis induced by meningococci. Efficiency of phagocytosis by U-937 cells was significantly higher for the noninvasive than invasive strains. Different behaviour of invasive and noninvasive strains of N. meningitidis observed during 4 hours of interactions with epithelial cells and monocytes/macrophages reflects well the higher pathogenic potential of invasive bacteria.
Kalmusova
J
J
National Institute of Public Health, Prague, Czech Republic.
Novotny
J
J
Hulinska
D
D
Musilek
M
M
Kriz
P
P
D000818:Animals; D004847:Epithelial Cells / Q000382:microbiology*; D000882:Haplorhini; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D008589:Meningococcal Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000382:microbiology; D051379:Mice; D009000:Monocytes / Q000382:microbiology* / Q000648:ultrastructure; D009345:Neisseria meningitidis / Q000472:pathogenicity*; D010587:Phagocytosis; D010614:Pharynx / Q000382:microbiology; D020298:U937 Cells