title: Effect of short interspersed element sequences on the integration and expression of a reporter gene in the preimplantation-stage mouse embryos.
abstract: Based on the assumption that foreign DNA sequences may have increased chance of integration into the host genome if they are flanked by high copy-numbered genomic sequences such as SINEs (short interspersed elements), we investigated the integration frequency of Lac Z reporter gene flanked by a fused B1/B2 in an in vivo system using pronuclear microinjection technique in the mouse. The SINE-flanked DNA showed a 4-fold increased integration frequency of the reporter gene than the control DNA (63% vs. 16%). Moreover, the level of beta-galactosidase expression, estimated from the X-Gal staining intensity in transgenic embryos, was greatly higher in SINE-carrying DNA. These results suggest that the SINE sequences can serve a very useful tool in improving the efficiency of current transgenic animal technology.
authors.affiliation: Animal Development Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejon, South Korea.
authors.forename: Y K
authors.initials: YK
authors.lastname: Kang
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: Y I
authors.initials: YI
authors.lastname: Yeom
authors.forename: Y M
authors.initials: YM
authors.lastname: Han
authors.forename: A S
authors.initials: AS
authors.lastname: Chung
authors.forename: K K
authors.initials: KK
authors.lastname: Lee
mesh_terms: D000818:Animals; D004278:DNA, Superhelical; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D051379:Mice; D008845:Microinjections; D011247:Pregnancy; D020086:Short Interspersed Nucleotide Elements; D019076:Transgenes; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D004278:DNA, Superhelical; D001616:beta-Galactosidase