title: Mercury distribution in the squirrel monkey retina after in Utero exposure to mercury vapor.
abstract: Pregnant squirrel monkeys were exposed to mercury vapor during approximately 2/3 of a pregnancy, at a concentration of 0.5 or 1 mg Hg/m(3) air for 4 or 7 h a day, 5 days a week. The offspring were sacrificed at different ages (gestational week 16 to 5 years). The eyes were enucleated and horizontal sections of the retina, comprising the optic disc and the fovea, were processed for autometallographic (AMG) silver enhancement. The AMG mercury distribution was mapped using light and epipolarization microscopy. In young offspring (16-week-old fetus to 3 days old), mercury was detected mainly in the optic nerve, retinal pigment epithelium, inner plexiform layer, vessel walls, and ganglion cells. Three and a half months later, the amount of visualized mercury had decreased in all areas except for the retinal pigment epithelium. In adult monkeys that had survived for 2 to 5 years, only a faint AMG staining was seen in the retinal pigment epithelium, the optic nerve, and in some vessel walls. In conclusion, in offspring sacrificed in utero or shortly after birth, the structures accumulating mercury were the same as those which accumulate mercury following direct exposure through the lungs, as reported previously (K. Warfvinge and A. Bruun, 1996, Toxicology 107, 189-200), although the amount of AMG staining was less in transplacental animals. This demonstrates that inorganic mercury penetrates the blood-retina barrier. In monkeys that had survived 3 to 5 years, only tiny amounts of mercury were detected, which is in contrast to findings from direct exposure, in which large amounts were still found 3 years after exposure. This may suggest that the elimination process in the retina is more efficient in young animals, but a possible adverse effect of mercury on retinal development cannot be ruled out.
authors.affiliation: Department of Ophthalmology, University Hospital of Lund, Lund, S-221 85, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Warfvinge
authors.forename: A
authors.initials: A
authors.lastname: Bruun
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001813:Blood-Retinal Barrier; D005260:Female; D019570:Inhalation Exposure; D018811:Maternal Exposure; D008628:Mercury / Q000008:administration & dosage / Q000009:adverse effects / Q000493:pharmacokinetics*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012160:Retina / Q000737:chemistry* / Q000473:pathology; D012453:Saimiri; D014018:Tissue Distribution; D014835:Volatilization
chemical_list: D008628:Mercury