Simultaneous detection of hepatitis B, C, and G viral genomes by multiplex PCR method.
We established a multiplex polymerase chain reaction (PCR) method for simultaneous detection of hepatitis B, C, and G viral genomes. The levels of concordance with the data obtained by conventional single PCR method were 100% for single infection, 98 to 100% for double infections, and 92% for triple infections. This method is not only suited to rapid, large-scale epidemiological screening and clinical diagnosis of those virus infections occurring alone or in combination, but is also time- and cost-effective.
Konomi
N
N
Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Yamaguchi
M
M
Naito
H
H
Aiba
N
N
Saito
T
T
Arakawa
Y
Y
Abe
K
K
D003362:Cost-Benefit Analysis; D018067:Flaviviridae / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000175:diagnosis / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012367:RNA, Viral / Q000737:chemistry; D013997:Time Factors
D012367:RNA, Viral