Increase of GAP-43 expression following kainic acid injection into whisker barrel cortex.
Plasticity after microinjection of kainic acid (KA) into the adult rat whisker barrel cortex was investigated with immunohistochemical staining of phosphorylated growth-associated protein (GAP)-43. After mapping the barrel cortex with the technique of intrinsic signal optical imaging, a small volume of KA was injected into one barrel. Rats were sacrificed at 2 days, 3 days, 1 week, and 6 weeks after lesioning. GAP-43 staining demonstrated intense immunoreactivity (IR) at the injected barrel which spread to the inter-barrel septa and the surrounding barrels. Elevated IR of GAP-43 was visible 2 days after KA injection, and increased gradually at least 6 weeks following the lesion. This model has the possibility of offering a simple and reliable tool for studying cortical plasticity.
Yamamoto
T
T
Department of Neurosurgery, State University of New York, Upstate Medical University, Syracuse 13210, USA.
Nguyen
T T
TT
Stevens
R T
RT
Meiri
K F
KF
Hodge
C J
CJ
D000818:Animals; D019922:GAP-43 Protein / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D007267:Injections; D007608:Kainic Acid / Q000494:pharmacology*; D009473:Neuronal Plasticity / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000187:drug effects* / Q000378:metabolism*; D013194:Staining and Labeling; D014738:Vibrissae / Q000502:physiology*
D019922:GAP-43 Protein; D007608:Kainic Acid