title: Cholesterol-lowering effects of guar gum: changes in bile acid pools and intestinal reabsorption.
abstract: Soluble fibers such as guar gum (GG) may exert cholesterol-lowering effects. It is generally accepted that bile acid (BA) reabsorption in portal blood is reduced, thus limiting the capacity of BA to down-regulate liver cholesterol 7alpha-hydroxylase, the rate-limiting enzyme of BA synthesis. In the present work, rats were adapted to fiber-free (FF) or 5% GG diets (supplemented or not with 0.25% cholesterol), to investigate various aspects of enterohepatic BA cycling. GG in the diet at a level of 5% elicited a significant lowering of plasma cholesterol during the absorptive period, in cholesterol-free (-13%) or 0.25% cholesterol (-20%) diet conditions. In rats adapted to the GG diets, the small intestinal and cecal BA pools and the ileal vein-artery difference for BA were markedly enhanced; reabsorption in the cecal vein was also enhanced in these rats. [14C]Taurocholate absorption, determined in perfused ileal segments, was not significantly different in rats adapted to the FF or GG diet, suggesting that a greater flux of BA in the ileum might support a greater ileal BA reabsorption in rats adapted to the GG diet. In contrast, capacities for [14C]cholate absorption from the cecum at pH 6.5 were higher in rats adapted to the GG diet than to the FF diet. Acidification of the bulk medium in isolated cecum (from pH 7.1 down to pH 6.5 or 5.8) or addition of 100 mM volatile fatty acids was also found to stimulate cecal [14C]cholate absorption. These factors could contribute to accelerated cecal BA absorption in rats fed the GG diet. The effects of GG on steroid fecal excretion thus appear to accompany a greater intestinal BA absorption and portal flux to the liver. These results suggest that some mechanisms invoked to explain cholesterol-lowering effect of fibers should be reconsidered.
authors.affiliation: Unité Maladies Métaboliques & Micronutriments, INRA de Theix, St-Genès-Champanelle, France.
authors.forename: S
authors.initials: S
authors.lastname: Moriceau
authors.forename: C
authors.initials: C
authors.lastname: Besson
authors.forename: M A
authors.initials: MA
authors.lastname: Levrat
authors.forename: C
authors.initials: C
authors.lastname: Moundras
authors.forename: C
authors.initials: C
authors.lastname: Rémésy
authors.forename: C
authors.initials: C
authors.lastname: Morand
authors.forename: C
authors.initials: C
authors.lastname: Demigné
mesh_terms: D000818:Animals; D001158:Arteries; D001647:Bile Acids and Salts / Q000097:blood / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002432:Cecum / Q000098:blood supply / Q000378:metabolism; D002784:Cholesterol / Q000097:blood*; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004043:Dietary Fiber / Q000494:pharmacology*; D005685:Galactans / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007082:Ileum / Q000098:blood supply; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008351:Mannans / Q000494:pharmacology*; D053149:Plant Gums; D051381:Rats; D017208:Rats, Wistar; D013656:Taurocholic Acid / Q000378:metabolism; D014680:Veins
chemical_list: D001647:Bile Acids and Salts; D002250:Carbon Radioisotopes; D002791:Cholesterol, Dietary; D004043:Dietary Fiber; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D013656:Taurocholic Acid; D002784:Cholesterol; C007894:guar gum