Diagnosis of quantitative mitochondrial DNA defects by rapidly prepared whole mitochondrial DNA probe.
Analysis of disease-causing mutations in mitochondria genome requires rapid and reliable genetic approaches. However, the preparation of mitochondrial DNA (mtDNA) probe used for the determination of quantitative and qualitative mtDNA defects is time-consuming, cumbersome, and requires complicated instrumentation. To overcome the difficulties encountered during isolation and purification of mtDNA, the authors developed an alternative method based on polymerase chain reaction (PCR) amplification of whole mtDNA genome. In this study, they show that PCR-amplified and fluorescein-labeled mtDNA probe makes it possible, through Southern blot analysis, to identify quantitative defect of mtDNA. The results indicate that mtDNA probe can be prepared rapidly by PCR amplification and used to determine the level of mtDNA in the patients with mitochondrial diseases.
Seyrantepe
V
V
Department of Medical Biology, Hacettepe University, Faculty of Medicine, Ihsan Dogramaci Children's Hospital, Ankara, Turkey.
Topaloglu
H
H
D015139:Blotting, Southern; D002478:Cells, Cultured; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes; D004272:DNA, Mitochondrial / Q000032:analysis*; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D008931:Mitochondria, Muscle / Q000235:genetics; D017240:Mitochondrial Myopathies / Q000175:diagnosis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000473:pathology
D017931:DNA Primers; D015342:DNA Probes; D004272:DNA, Mitochondrial