The immune response of mice and cynomolgus monkeys to macaque mucin 1-mannan.
Mice immunised with human epithelial mucin MUC1 coupled to oxidised mannan produce MUC1 specific MHC Class 1 restricted CD8(+) cytotoxic T cells and are completely protected from the development of MUC1(+) tumours; such therapy may be applicable to humans. In this light we describe pre-clinical studies in cynomolgus monkeys (Macaca fascicularis), to test the efficacy of mannan-MUC1 in higher primates. Monkey MUC1 genomic clones were isolated from a macaque library, peptides and fusion protein synthesised and mice and monkeys immunised with macaque MUC1-mannan. In mice CTL responses were induced (as has been found with human MUC1 mannan conjugates), but in contrast monkeys produced a humoral response, with no T cell proliferative, cytotoxic responses or CTLp found. In spite of the presence of anti-MUC1 auto-antibodies, there was no toxicity or induction of autoimmunity.
Vaughan
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HA
The Austin Research Institute, A&RMC, StudleyRoad, Vic 3084, Heidelberg, Australia.
Ho
D W
DW
Karanikas
V
V
Sandrin
M S
MS
McKenzie
I F
IF
Pietersz
G A
GA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003429:Cross Reactions; D005091:Exons; D007114:Immunization; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D008351:Mannans / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011110:Polymorphism, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D020080:Tandem Repeat Sequences
D008351:Mannans; D018396:Mucin-1