Microtubule-associated protein MAP2 preferentially binds to a dA/dT sequence present in mouse satellite DNA.
Microtubule-associated protein MAP2 binds to the Sau96.1 restriction monomer fragment of mouse satellite DNA. This fragment is also present in a lower proportion in bulk DNA. The digestion of MAP2-Sau96.1 fragment complex by DNase results in the protection of certain nucleotide sequences. The sequence poly(dA)4/poly(dT)4 is mainly protected against DNase digestion.
Avila
J
J
Centro de Biologia Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Montejo de Garcini
E
E
Wandosell
F
F
Villasante
A
A
Sogo
J M
JM
Villanueva
N
N
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004276:DNA, Satellite / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D013552:Swine
D004276:DNA, Satellite; D008869:Microtubule-Associated Proteins; D011089:Polydeoxyribonucleotides; C084838:polydeoxyadenylic acid-polythymidylic acid; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases