Cloning and characterization of a novel cDNA sequence encoding the precursor of a novel venom peptide (BmKbpp) related to a bradykinin-potentiating peptide from Chinese scorpion Buthus martensii Karsch.
Based on the amino acid sequence of a bradykinin-potentiating peptide (Bpp) (peptide K-12) from scorpion Buthus occitanus, a full-length cDNA sequence encoding the precursor of a novel venom peptide (named BmKbpp) related to this Bpp, has been isolated and analyzed. The cDNA encodes a precursor of 72 amino acid residues, including a signal peptide of 22 residues and an extra Arg-Arg-Arg tail at the C-terminal end of the precursor, which have to be removed in the processing step. The C-terminal region (21 residues) of the precursor is homologous (57% identical) with the sequence of peptide K-12. Thus, according to the primary structure of the BmKbpp precursor, there may be a propeptide between the signal peptide and the putative mature BmKbpp at the C-terminal region of the precursor.
Zeng
X C
XC
Department of Virology and Molecular Biology, School of Life Science, Wuhan University, People's Republic of China.
Li
W X
WX
Peng
F
F
Zhu
Z H
ZH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D015723:Gene Library; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics*; D012604:Scorpion Venoms / Q000737:chemistry* / Q000235:genetics*; D012605:Scorpions / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C416855:BmKbpp protein precursor; C416854:BmKbpp protein, Buthus martensii Karsch; D018076:DNA, Complementary; D009842:Oligopeptides; D011498:Protein Precursors; D012604:Scorpion Venoms; C006791:bradykinin potentiating factors