Purification and characterization of Sa-lrp, a DNA-binding protein from the extreme thermoacidophilic archaeon Sulfolobus acidocaldarius homologous to the bacterial global transcriptional regulator Lrp.
Archaea, constituting the third primary domain of life, harbor a basal transcription apparatus of the eukaryotic type, whereas curiously, a large fraction of the potential transcription regulation factors appear to be of the bacterial type. To date, little information is available on these predicted regulators and on the intriguing interplay that necessarily has to occur with the transcription machinery. Here, we focus on Sa-lrp of the extremely thermoacidophilic crenarchaeote Sulfolobus acidocaldarius, encoding an archaeal homologue of the Escherichia coli leucine-responsive regulatory protein Lrp, a global transcriptional regulator and genome organizer. Sa-lrp was shown to produce a monocistronic mRNA that was more abundant in the stationary-growth phase and produced in smaller amounts in complex medium, this down regulation being leucine independent. We report on Sa-Lrp protein purification from S. acidocaldarius and from recombinant E. coli, both identified by N-terminal amino acid sequence determination. Recombinant Sa-Lrp was shown to be homotetrameric and to bind to its own control region; this binding proved to be leucine independent and was stimulated at high temperatures. Interference binding experiments suggested an important role for minor groove recognition in the Sa-Lrp-DNA complex formation, and mutant analysis indicated the importance for DNA binding of the potential helix-turn-helix motif present at the N terminus of Sa-Lrp. The DNA-binding capacity of purified Sa-Lrp was found to be more resistant to irreversible heat inactivation in the presence of L-leucine, suggesting a potential physiological role of the amino acid as a cofactor.
Enoru-Eta
J
J
Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, and Department of Microbiology, The Flanders Interuniversity Institute for Biotechnology, Université Libre de Bruxelles, Brussels, Belgium.
Gigot
D
D
Thia-Toong
T L
TL
Glansdorff
N
N
Charlier
D
D
D019943:Amino Acid Substitution; D019843:Archaeal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D018983:DNA Footprinting; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005796:Genes; D018257:Helix-Loop-Helix Motifs; D051756:Leucine-Responsive Regulatory Protein; D008024:Ligands; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic; D019642:RNA, Archaeal; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016945:Sulfolobus acidocaldarius / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D019843:Archaeal Proteins; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D008024:Ligands; C497278:Lrp protein, E coli; D019642:RNA, Archaeal; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D051756:Leucine-Responsive Regulatory Protein; D004247:DNA; D003850:Deoxyribonuclease I