Analysis of the function of a putative 2,3-diphosphoglyceric acid-dependent phosphoglycerate mutase from Bacillus subtilis.
A Bacillus subtilis gene termed yhfR encodes the only B. subtilis protein with significant sequence similarity to 2, 3-diphosphoglycerate-dependent phosphoglycerate mutases (dPGM). This gene is expressed at a low level during growth and sporulation, but deletion of yhfR had no effect on growth, sporulation, or spore germination and outgrowth. YhfR was expressed in and partially purified from Escherichia coli but had little if any PGM activity and gave no detectable PGM activity in B. subtilis. These data indicate that B. subtilis does not require YhfR and most likely does not require a dPGM.
Pearson
C L
CL
Department of Biochemistry, University of Connecticut Health Center, Farmington 06032, USA.
Loshon
C A
CA
Pedersen
L B
LB
Setlow
B
B
Setlow
P
P
D019794:2,3-Diphosphoglycerate / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D010736:Phosphoglycerate Mutase / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013171:Spores, Bacterial
D011993:Recombinant Fusion Proteins; D019794:2,3-Diphosphoglycerate; D010736:Phosphoglycerate Mutase