Determination of food constituents based on SFE: applications to vitamins A and E in meat and milk.
A method has been developed for the determination of vitamins A and E in food using supercritical fluid extraction (SFE), applying liquid or solid trapping, with an accuracy equal to conventional solvent extraction methods. Under optimal conditions, using methanol modified carbon dioxide as a supercritical fluid, Hydromatrix as a water adsorbent, and with a small amount of ascorbic acid and methanol added to the sample, the extraction time is reduced to 80 min. This time is considerably shorter than in conventional methods. Other advantages are the reduction of manual manipulations leading to lower labour costs and reduced consumption of organic solvents in the sample preparation step.
Berg
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Swedish Meats R&D, Box 504, 244 24, Kävlinge, Sweden.
Turner
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C
Dahlberg
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Mathiasson
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L
D000818:Animals; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry; D013997:Time Factors; D014801:Vitamin A / Q000032:analysis* / Q000302:isolation & purification; D014810:Vitamin E / Q000032:analysis* / Q000302:isolation & purification
D012503:Saponins; D014801:Vitamin A; D014810:Vitamin E