Microfabricated electrophoresis chips for simultaneous bioassays of glucose, uric acid, ascorbic acid, and acetaminophen.
A micromachined capillary electrophoresis chip is described for simultaneous measurements of glucose, ascorbic acid, acetaminophen, and uric acid. Fluid control is used to mix the sample and enzyme glucose oxidase (GOx). The enzymatic reaction, a catalyzed aerobic oxidation of glucose to gluconic acid and hydrogen peroxide, occurs along the separation channel. The enzymatically liberated neutral peroxide species is separated electrophoretically from the anionic uric and ascorbic acids in the separation/reaction channel. The three oxidizable species are detected at the downstream gold-coated thick-film amperometric detector at different migration times. Glucose can be detected within less than 100 s, and detection of all electroactive constituents is carried out within 4 min. Measurements of glucose in the presence of acetaminophen, a neutral compound, are accomplished by comparing the responses in the presence and absence of GOx in the running buffer. The reproducibility of the on-chip glucose measurements is improved greatly by using uric acid as an internal standard. Factors influencing the performance, including the GOx concentration, field strength, and detection potential, are optimized. Such coupling of enzymatic assays with electrophoretic separations on a microchip platform holds great promise for rapid testing of metabolites (such as glucose or lactate), as well as for the introduction of high-speed clinical microanalyzers based on multichannel chips.
Wang
J
J
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003, USA.
Chatrathi
M P
MP
Tian
B
B
Polsky
R
R
D000082:Acetaminophen / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000295:instrumentation; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D008838:Microcomputers; D008839:Microelectrodes; D014527:Uric Acid / Q000032:analysis*
D004800:Enzymes, Immobilized; D014527:Uric Acid; D000082:Acetaminophen; D005947:Glucose; D001205:Ascorbic Acid