Identification of a novel secretory leukocyte protease inhibitor-binding protein involved in membrane phospholipid movement.
Previous studies have suggested that human salivary secretory leukocyte protease inhibitor (SLPI) inhibits HIV-1 by binding to a host cell surface protein of unknown identity. Using the yeast two-hybrid assay, we identified a gene sequence encoding a novel SLPI-binding protein (SLPI-BP). The 1.5-kb cDNA encodes a 318-amino acid protein with a predicted transmembrane segment near the C-terminus. Sequence analysis revealed that SLPI-BP is the human scramblase protein that is involved in the movement of membrane phospholipids. Co-expression of SLPI and SLPI-BP followed by an S-protein pulldown assay confirmed the specific interaction between these two proteins. Our data represent the first report for the identity of SLPI-BP.
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Divisions of Basic Sciences and of Restorative and Prosthodontic Sciences, New York University College of Dentistry, New York, NY 10010, USA. cct1@is3.nyu.edu
Tseng
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D001483:Base Sequence; D015497:HIV-1 / Q000378:metabolism; D006801:Humans; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D053481:Secretory Leukocyte Peptidase Inhibitor
D008563:Membrane Lipids; D010743:Phospholipids; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; C506814:SLPI protein, human; D012471:Salivary Proteins and Peptides; D053481:Secretory Leukocyte Peptidase Inhibitor