Quantitative determination of Cu-thionein from human fluids with application of solid-phase extraction on covalent affinity chromatography with thiol-disulphide interchange support.
The aim of our investigation was to carry out quantitative isolation of Cu-thionein (Cu-Th) in human body fluids (urine, plasma and breast milk) in order to determine the level of exposure to heavy metals. In the experiment covalent affinity chromatography with thiol-disulphide interchange gel (CAC-TDI) was used as a solid phase extraction (SPE) support for preconcentration of Cu-thionein (Cu-Th) protein and Cu bonded with MT from water and human fluids samples. The present paper is a continuation of early experiments on the quantitation of Hg-thionein (Hg-Th), Cd-thionein (Cd-Th) and Zn-thionein (Zn-Th) in human body fluids such as urine, plasma and breast milk.
Kabziński
A K
AK
University of Lódź, Faculty of Physics and Chemistry, Department of General and Inorganic Chemistry, Poland.
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004220:Disulfides; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008297:Male; D008668:Metallothionein / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D008895:Milk, Human / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013438:Sulfhydryl Compounds
D004220:Disulfides; D013438:Sulfhydryl Compounds; C007121:copper thionein; D008668:Metallothionein