Cytopathogenic effect of Trichomonas vaginalis on human vaginal epithelial cells cultured in vitro.
In this study we established human vaginal epithelial cells (hVECs) in culture and evaluated their interaction with Trichomonas vaginalis parasites to complement previous studies using other cell types. Primary cultures of hVECs were established. Contaminating fibroblasts were separated from epithelial cells by differential trypsinization. Specific antibody staining revealed that over 92% of cells in hVEC monolayers were epithelial cells. T. vaginalis adhered to hVECs and produced severe cytotoxic effects resulting in obliteration of the monolayer within 24 h. Adherence and cytotoxicity were not observed when T. vaginalis was exposed to human vaginal fibroblasts or bovine vaginal epithelial cells. Likewise, the bovine parasite Tritrichomonas foetus had no cytotoxic effects on hVECs. We concluded that the interaction between T. vaginalis and hVECs is both cell specific (limited to epithelial cells and not vaginal fibroblasts) and species specific (limited to human vaginal cells and not bovine cells). Pretreatment of T. vaginalis with metronidazole or periodate abolished the adhesion of parasites to cell monolayers and the cytotoxic effect, suggesting involvement of carbohydrate-containing molecules in these processes. Different clinical isolates of T. vaginalis caused damage to cultured cells at different rates. Parasites separated from the vaginal cell monolayer by a permeable membrane did not produce a cytopathic effect, suggesting contact-dependent cytotoxicity.
Gilbert
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RO
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA. rog1@cornell.edu
Elia
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G
Beach
D H
DH
Klaessig
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S
Singh
B N
BN
D000818:Animals; D000994:Antitrichomonal Agents / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000469:parasitology / Q000473:pathology; D005260:Female; D006801:Humans; D008795:Metronidazole / Q000494:pharmacology; D013045:Species Specificity; D014244:Trichomonas / Q000472:pathogenicity; D014246:Trichomonas vaginalis / Q000187:drug effects / Q000472:pathogenicity*; D014621:Vagina / Q000469:parasitology* / Q000473:pathology
D000994:Antitrichomonal Agents; D008795:Metronidazole