title: Changes in superoxide dismutase activity and mRNA in vivo after short-term supplementation with trilinolein in rats.
abstract: BACKGROUND
  Oxidative damage plays a central role in atherogenesis and antioxidation defense mechanisms may prevent atherosclerosis. This study evaluated the effect of short-term supplementation of the natural lipophilic antioxidant trilinolein on superoxide dismutase (SOD) activity and SOD-mRNA gene expression in vivo in rat vital organs.
  
  
  METHODS
  Male Wistar rats (n = 8) were injected intraperitoneally with trilinolein (1 mM/ml/kg/day in 0.5% ethanol) daily for three consecutive days. Two control groups (n = 8) were administered saline or 0.5% ethanol in saline, respectively, for three days.
  
  
  RESULTS
  Assay of SOD activity and SOD-mRNA by Northern blotting in rat liver, spleen and brain showed significant increases in SOD activity and increased SOD-mRNA gene expression.
  
  
  CONCLUSIONS
  The natural lipophilic antioxidant trilinolein potentiates the SOD antioxidation defense mechanism and increases gene expression of SOD-mRNA after short-term supplementation in rats.
authors.affiliation: Department of Medicine, Taipei Medical College, Taiwan, ROC.
authors.forename: P
authors.initials: P
authors.lastname: Chan
authors.forename: W P
authors.initials: WP
authors.lastname: Huang
authors.forename: J C
authors.initials: JC
authors.lastname: Liu
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chen
authors.forename: J T
authors.initials: JT
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005609:Free Radicals; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D012333:RNA, Messenger; D014280:Triglycerides; D013482:Superoxide Dismutase; C009564:trilinolein