title: In vivo comparison of CHX-DTPA ligand isomers in athymic mice bearing carcinoma xenografts.
abstract: Monoclonal antibodies (MAbs) labeled with radiometallonuclides via metal chelators are being investigated in the laboratory for use in clinical trials. The biodistribution of 111In- and 88Y-labeled antibody (MAb B72.3) using two isomeric forms (CHX-A and CHX-B) of the 2-(p-isothiocyanatobenzyl)-cyclohexyl-DTPA was compared in athymic mice bearing LS-174T tumors, human colon carcinoma xenografts. CHX-(A or B)-125I-DTPA-B72.3 was co-injected in all athymic mice to assess if the chelate conjugation altered the properties of MAb B72.3. In vitro studies demonstrated maintenance of integrity and immunoreactivity for both radioimmunoconjugates. The in vivo analysis, however, indicated major differences between the two isomer forms. In fact, the 88Y-CHX-A-DTPA radioimmunoconjugate demonstrated over the 7-day study period, a more efficient and stable tumor localization as well as a slower blood clearance rate than the CHX-B-DTPA chelate conjugate, suggesting a greater in vivo stability. Differences were also evident in critical normal organ uptake: no significant increase in liver- and spleen- or bone-to-blood ratios was observed when the CHX-A-DTPA chelate was labeled with indium or yttrium. The results described here demonstrate that the CHX-A-DTPA chelate conjugate can be considered more suitable than the CHX-B-DTPA isomer form when radiometallonuclides are coupled to an MAb.
authors.affiliation: Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, MD 20892, USA.
authors.forename: M
authors.initials: M
authors.lastname: Roselli
authors.forename: D E
authors.initials: DE
authors.lastname: Milenic
authors.forename: M W
authors.initials: MW
authors.lastname: Brechbiel
authors.forename: S
authors.initials: S
authors.lastname: Mirzadeh
authors.forename: C G
authors.initials: CG
authors.lastname: Pippin
authors.forename: O A
authors.initials: OA
authors.lastname: Gansow
authors.forename: D
authors.initials: D
authors.lastname: Colcher
authors.forename: J
authors.initials: J
authors.lastname: Schlom
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics / Q000627:therapeutic use; D003110:Colonic Neoplasms / Q000532:radiotherapy*; D006801:Humans; D007205:Indium Radioisotopes / Q000493:pharmacokinetics* / Q000627:therapeutic use; D007536:Isomerism; D017879:Isothiocyanates; D008024:Ligands; D051379:Mice; D008819:Mice, Nude; D004369:Pentetic Acid / Q000031:analogs & derivatives*; D016499:Radioimmunotherapy; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics* / Q000627:therapeutic use; D014018:Tissue Distribution; D014183:Transplantation, Heterologous; D015021:Yttrium Radioisotopes / Q000493:pharmacokinetics* / Q000627:therapeutic use
chemical_list: D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D017879:Isothiocyanates; D008024:Ligands; D019275:Radiopharmaceuticals; D015021:Yttrium Radioisotopes; C085678:N-(2-amino-3-(4-isothiocyanatophenyl)propyl)cyclohexane-1,2-diamine-N,N',N',N'',N''-pentaacetic acid; D004369:Pentetic Acid