title: Binding and activation of human plasminogen by Mycobacterium tuberculosis.
abstract: The first evidence of the interaction of Mycobacterium tuberculosis with the plasminogen system is herein reported. By FACScan analysis and affinity blotting, lysine-dependent binding of plasminogen to M. tuberculosis was demonstrated. The binding molecules were 30-, 60-, and 66-kDa proteins present in cell wall and soluble protein extracts. The activation of plasminogen, which occurred only in presence of fibrin and was not inhibited by the host serpin, alpha(2)-antiplasmin, was also demonstrated.
authors.affiliation: Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, C.P. 04510 Mexico City D.F., Mexico.
authors.forename: V
authors.initials: V
authors.lastname: Monroy
authors.forename: A
authors.initials: A
authors.lastname: Amador
authors.forename: B
authors.initials: B
authors.lastname: Ruiz
authors.forename: P
authors.initials: P
authors.lastname: Espinoza-Cueto
authors.forename: W
authors.initials: W
authors.lastname: Xolalpa
authors.forename: R
authors.initials: R
authors.lastname: Mancilla
authors.forename: C
authors.initials: C
authors.lastname: Espitia
mesh_terms: D005337:Fibrin / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009169:Mycobacterium tuberculosis / Q000378:metabolism / Q000472:pathogenicity*; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D055293:Receptors, Urokinase Plasminogen Activator; D014376:Tuberculosis / Q000209:etiology; D000979:alpha-2-Antiplasmin / Q000494:pharmacology
chemical_list: C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D000979:alpha-2-Antiplasmin; D005337:Fibrin; D010958:Plasminogen