A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 2: competitive binding.
A qualitative assay for the evaluation of soluble ligands of the nuclear import receptor karyopherin alpha has been developed. The assay relies on competition with an immobilized ligand, the nuclear localization signal (NLS) from nucleoplasmin, for binding to the receptor, which is detected by an enzyme-linked colorimetric method.
Park
S B
SB
Department of Chemistry, Texas A&M University, College Station 77842-3012, USA.
Reedy
B M
BM
Standaert
R F
RF
D000595:Amino Acid Sequence; D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry; D001667:Binding, Competitive / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D003124:Colorimetry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D056489:Nucleoplasmins; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology; D010750:Phosphoproteins / Q000737:chemistry; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects / Q000378:metabolism*; D028901:alpha Karyopherins
D000952:Antigens, Polyomavirus Transforming; D009687:Nuclear Proteins; D056489:Nucleoplasmins; D010455:Peptides; D010750:Phosphoproteins; D018160:Receptors, Cytoplasmic and Nuclear; D028901:alpha Karyopherins