title: Detection of hematogenic tumor cell dissemination in patients undergoing resection of liver metastases of colorectal cancer.
abstract: OBJECTIVE
  To determine the extent of pre- and intraoperative hematogenic tumor cell dissemination in patients undergoing liver resection for metastatic colorectal cancer.
  
  
  BACKGROUND
  For patients with hepatic metastases of colorectal cancer, liver resection is the only potentially curative therapy. However, 38% to 53% of patients develop extrahepatic tumor recurrence, probably caused by tumor cells disseminated before or during surgery not detected by current staging systems.
  
  
  METHODS
  Blood samples harvested before, during, and after surgery from 41 patients and bone marrow samples from 30 patients undergoing resection of liver metastases of colorectal cancer were analyzed for disseminated tumor cells using cytokeratin 20 reverse transcriptase-polymerase chain reaction.
  
  
  RESULTS
  Tumor cells were detected in the blood samples of 26 of the 41 patients (63.4%) and in the bone marrow samples of 8 of the 30 patients (26.7%). Tumor cells were detected significantly more often during surgery than before or after surgery. Intraoperative tumor cell dissemination was detected in 41.7% of patients undergoing resection of two or more liver segments but only 14.3% of patients undergoing resection of one liver segment. Compared with resection of primary colorectal cancer, major liver resection carries an increased risk for intraoperative tumor cell dissemination.
  
  
  CONCLUSIONS
  Detection of disseminated tumor cells in patients undergoing liver resection for metastases of colorectal cancer using cytokeratin 20 reverse transcriptase-polymerase chain reaction might help to identify patients at high risk for tumor recurrence who may benefit from adjuvant therapy. Major liver resection of metastases leads to frequent intraoperative tumor cell shedding, possibly preventable by alternative surgical strategies.
authors.affiliation: Division for Molecular Diagnostics and Therapy and the Division for Surgical Oncology, the Department of Surgery, University of Heidelberg, and the Central Unit Biostatistics, German Cancer Research Center, Heidelberg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Weitz
authors.forename: M
authors.initials: M
authors.lastname: Koch
authors.forename: P
authors.initials: P
authors.lastname: Kienle
authors.forename: A
authors.initials: A
authors.lastname: Schrödel
authors.forename: F
authors.initials: F
authors.lastname: Willeke
authors.forename: A
authors.initials: A
authors.lastname: Benner
authors.forename: T
authors.initials: T
authors.lastname: Lehnert
authors.forename: C
authors.initials: C
authors.lastname: Herfarth
authors.forename: M
authors.initials: M
authors.lastname: von Knebel Doeberitz
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D001853:Bone Marrow / Q000473:pathology; D015179:Colorectal Neoplasms / Q000473:pathology*; D005260:Female; D006498:Hepatectomy; D006801:Humans; D007381:Intermediate Filament Proteins; D007432:Intraoperative Period; D053554:Keratin-20; D008113:Liver Neoplasms / Q000473:pathology* / Q000556:secondary / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D007381:Intermediate Filament Proteins; C507499:KRT20 protein, human; D053554:Keratin-20