Preventive aspirin treatment of streptozotocin induced diabetes: blockage of oxidative status and revertion of heme enzymes inhibition.
Some late complications of diabetes are associated with alterations in the structure and function of proteins due to glycation and free radicals generation. Aspirin inhibits protein glycation by acetylation of free amino groups. In the diabetic status, it was demonstrated that several enzymes of heme pathway were diminished. The aim of this work has been to investigate the in vivo effect of short and long term treatment with acetylsalicylic acid in streptozotocin induced diabetic mice. In both treatments, the acetylsalicylic acid prevented delta-aminolevulinic dehydratase and porphobilinogen deaminase inactivation in diabetic mice and blocked the accumulation of lipoperoxidative aldehydes. Catalase activity was significantly augmented in diabetic mice and the long term treatment with aspirin partially reverted it. We propose that oxidative stress might play an important role in streptozotocin induced diabetes. Our results suggest that aspirin can prevent some of the late complications of diabetes, lowering glucose concentration and probably inhibiting glycation by acetylation of protein amino groups.
Caballero
F
F
Department of Biological Chemistry, FCEN, University of Buenos Aires, Centro de Investigaciones sobre Porfirinas y Porfirias, Ciudad Universitaria, Pabell¿on II, 2do piso, 1428, Buenos Aires, Argentina.
Gerez
E
E
Batlle
A
A
Vazquez
E
E
D000818:Animals; D001241:Aspirin / Q000494:pharmacology / Q000627:therapeutic use*; D001786:Blood Glucose; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000201:enzymology / Q000517:prevention & control*; D004032:Diet; D004435:Eating / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006442:Glycated Hemoglobin / Q000032:analysis; D011163:Hydroxymethylbilane Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013311:Streptozocin
D001241:Aspirin; D001786:Blood Glucose; D002374:Catalase; D004791:Enzyme Inhibitors; D006442:Glycated Hemoglobin; D011163:Hydroxymethylbilane Synthase; D000623:Porphobilinogen Synthase; D013311:Streptozocin