title: Fabrication of an electrode-viologen-hydrogenase heterogeneous system and the electrochemical hydrogen evolution.
abstract: An indium tin oxide (ITO) electrode was chemically modified by one layer of viologen (VIO) derivative, which possessed a persistent and reproducible electrochemical response. A monolayer of a thermal stable hydrogenase from Thiocapsa roseopersicina was stabilized on a synthesized poly-L-lysine subphase surface and transferred onto the electrode for fabrication of an ITO-VIO-hydrogenase heterogeneous system. Electrochemical properties of both the ITO-VIO monolayer and the heterogeneous ITO-VIO-hydrogenase system have been investigated. Hydrogen evolution could be measured by potentiostating the VIO-hydrogenase-covered ITO electrode to "electroplate" [(VIO+)n]surf, and a large increase in hydrogen evolution was observed when using an electrolyte solution containing sodium dithionite. We discuss the possible electron transfer process.
authors.affiliation: National Institute of Advanced Interdisciplinary Research, AIST, Ibaraki, Japan.
authors.forename: D J
authors.initials: DJ
authors.lastname: Qian
authors.forename: C
authors.initials: C
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Noda
authors.forename: N A
authors.initials: NA
authors.lastname: Zorin
authors.forename: J
authors.initials: J
authors.lastname: Miyake
mesh_terms: D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006859:Hydrogen; D006864:Hydrogenase / Q000378:metabolism*; D011199:Potentiometry; D020586:Thiocapsa / Q000201:enzymology; D017971:Tin Compounds
chemical_list: D004800:Enzymes, Immobilized; D017971:Tin Compounds; C109984:indium tin oxide; D006859:Hydrogen; D006864:Hydrogenase