Molecular cloning of a cyclin-like protein associated with cyclin-dependent kinase 3 (cdk 3) in vivo.
cdk3 has been considered to be rate-limiting for cell cycle progression of mammalian cells while its precise function remains to be elucidated. To assess cdk3 function, a cDNA coding for a cyclin-like protein (designated as ik3-1 from an interactor-1 with cdk3) was isolated with the yeast two-hybrid system using a cyclin-dependent kinase 3 (cdk3) cDNA as bait. p70(ik3-1) (a 70-kDa protein designated as p70(ik3-1)) seems to belong to the cyclin family as its C-terminal domain composed of 124 amino acids resembles the highly conserved cyclin box. Coimmunoprecipitation indicated that p70(ik3-1) binds to p35(cdk3) in vivo. The ik3-1 gene may belong to a multigene family and is highly conserved during evolution. mRNA expression of ik3-1 was low in the early G1 phase, upregulated during G1 progression, maximal at a mid-late G1 point, and declined gradually thereafter, suggesting that it may work mainly in G1 phase.
Matsuoka
M
M
Department of Pharmacology, Keio University School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo, 160-8582, Japan. sakimatu@mc.med.keio.ac.jp
Matsuura
Y
Y
Semba
K
K
Nishimoto
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D002352:Carrier Proteins; D003001:Cloning, Molecular; D056849:Cyclin-Dependent Kinase 3; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D016193:G1 Phase; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010750:Phosphoproteins; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C486568:CABLES1 protein, human; C411580:Cables1 protein, mouse; D002352:Carrier Proteins; D016213:Cyclins; D018076:DNA, Complementary; D010750:Phosphoproteins; D012333:RNA, Messenger; C490914:Cdk3 protein, mouse; D056849:Cyclin-Dependent Kinase 3; D018844:Cyclin-Dependent Kinases