title: Molecular cloning of the gene encoding flavoredoxin, a flavoprotein from Desulfovibrio gigas.
abstract: Sulfate-reducing bacteria are rich in unique redox proteins and electron carriers that participate in a variety of essential pathways. Several studies have been carried out to characterize these proteins, but the structure and function of many are poorly understood. Many Desulfovibrio species can grow using hydrogen as the sole energy source, indicating that the oxidation of hydrogen with sulfite as the terminal electron acceptor is an energy-conserving mechanism. Flavoredoxin is an FMN-binding protein isolated from the sulfate-reducing bacteria Desulfovibrio gigas that participates in the reduction of bisulfite from hydrogen. Here we report the cloning and sequencing of the flavoredoxin gene. The derived amino acid sequence exhibits similarity to several flavoproteins which are members of a new family of flavin reductases suggested to bind FMN in a novel mode.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Agostinho
authors.forename: S
authors.initials: S
authors.lastname: Oliveira
authors.forename: M
authors.initials: M
authors.lastname: Broco
authors.forename: M Y
authors.initials: MY
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: LeGall
authors.forename: C
authors.initials: C
authors.lastname: Rodrigues-Pousada
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003901:Desulfovibrio / Q000201:enzymology / Q000235:genetics*; D038181:FMN Reductase; D005486:Flavin Mononucleotide / Q000378:metabolism; D005420:Flavoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013447:Sulfites / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005420:Flavoproteins; D013447:Sulfites; D005486:Flavin Mononucleotide; D006859:Hydrogen; D010088:Oxidoreductases; C410951:flavoredoxin; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; C042345:hydrogen sulfite