A flow injection chemiluminescence method using Cr(III) as a catalyst for determining hydrogen peroxide. Application to H(2)O(2) determination in cultures of microalgae.
Flow injection analysis has been applied to the determination of hydrogen peroxide produced by some different species of microalgae. The method is based on the luminol-H(2)O(2) chemiluminescence reaction using Cr(III) as a catalyst. Optimum experimental conditions for the method have been studied and trace amounts of hydrogen peroxide determined with detection limits of 4 10(-8) mol/L. The method using Cr(III) was compared with that using horseradish peroxidase as the catalyst.
Escobar
R
R
Department of Analytical Chemistry, University of Seville, Spain.
García-Domínguez
S
S
Guiraúm
A
A
Montes
O
O
Galván
F
F
de La Rosa
F F
FF
D002384:Catalysis; D002857:Chromium; D004492:Edetic Acid; D056890:Eukaryota / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D017022:Flow Injection Analysis / Q000379:methods*; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D008163:Luminescent Measurements; D008165:Luminol; D010777:Photochemistry
D002857:Chromium; D008165:Luminol; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase