[Integration via Tn10 and expression of Bti cryIVA gene in the chromosome DNA of flourescent Pseudomonas bacterial strains].
cryIVA gene of the Bacillus thuringiensis subsp. israelensis was subcloned into the Tn10 of a suicide transposon plasmid vector and a transposon plasmid pLF97A carrying cryIVA was constructed. By electroporation and transposition, cryIVA gene integrated into chromosome DNA of F.P.DE2. Thus the genetic engineered bacterial strain, F.P.DE202, was constructed. Southern blotting revealed that this integration was happened in different loci of the chromosome. Western blotting demonstrated that cryIVA was expressed in the engineered strain, and that the expressed product possesed the pesticidal ability against the third-instar larva of Bradysia odoriphaga.
Liu
G Q
GQ
College of Life Sciences, Nankai University, Tianjin.
Jiang
R Z
RZ
Zhang
Z L
ZL
D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D004731:Endotoxins / Q000235:genetics*; D006460:Hemolysin Proteins; D010572:Pest Control, Biological; D011549:Pseudomonas / Q000235:genetics*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004251:DNA Transposable Elements; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis