title: The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus 'Sir Winston Churchill'.
abstract: An HPLC with on-line coupled UV/MS-biochemical detection method for acetylcholinesterase (AChE) inhibitors in natural sources has been developed. The potential of this method is shown by the isolation of a new AChE inhibitor from the alcoholic extract of Narcissus 'Sir Winston Churchill'. Combining a prefractionation technique using centrifugal partition chromatography with the on-line HPLC-UV/MS-biochemical detection resulted in the isolation of the active compound that was identified as ungiminorine. This alkaloid shows a mild inhibitory effect on AChE.
authors.affiliation: Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
authors.forename: K
authors.initials: K
authors.lastname: Ingkaninan
authors.forename: A
authors.initials: A
authors.lastname: Hazekamp
authors.forename: C M
authors.initials: CM
authors.lastname: de Best
authors.forename: H
authors.initials: H
authors.lastname: Irth
authors.forename: U R
authors.initials: UR
authors.lastname: Tjaden
authors.forename: R
authors.initials: R
authors.lastname: van der Heijden
authors.forename: J
authors.initials: J
authors.lastname: van der Greef
authors.forename: R
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D047151:Amaryllidaceae Alkaloids; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005702:Galantamine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010944:Plants / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000470:Alkaloids; D047151:Amaryllidaceae Alkaloids; D002800:Cholinesterase Inhibitors; C412690:ungiminorine; D005702:Galantamine