title: BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3'.
abstract: The recognition sequence and cleavage positions of a new restriction endonuclease BTR:I isolated from Bacillus stearothermophilus SE-U62 have been determined. BTR:I belongs to a rare type IIQ of restriction endonucleases, which recognise non-palindromic nucleotide sequences and cleave DNA symmetrically within them.
authors.affiliation: SibEnzyme, 9 Ak.Timakov Str., Novosibirsk 630117, Russia.
authors.forename: S K
authors.initials: SK
authors.lastname: Degtyarev
authors.forename: O A
authors.initials: OA
authors.lastname: Belichenko
authors.forename: N A
authors.initials: NA
authors.lastname: Lebedeva
authors.forename: V S
authors.initials: VS
authors.lastname: Dedkov
authors.forename: M A
authors.initials: MA
authors.lastname: Abdurashitov
mesh_terms: D004279:DNA, Viral / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; C410662:endodeoxyribonuclease BtrI; D015252:Deoxyribonucleases, Type II Site-Specific