title: Radiation-induced apoptosis and cell cycle alterations in human carcinoma cell lines with different radiosensitivities.
abstract: Radiosensitivity of examined human neoplastic cell lines was assessed with the aid of MTT assay. Differences between radiosensitive and radioresistant human neoplastic cell lines were as follow: a) radiation-induced apoptosis detected by flow cytometry was apparent in the most radiosensitive (i.e. CH-1 ovarian carcinoma cell line), but not in the radioresistant (i.e. SKOV-3 ovarian carcinoma) cell lines, b) radiation-induced G2/M arrest appeared early after irradiation (6 hours) in both the radioresistant SKOV-3 cells and in the radiosensitive CH-1 human ovarian carcinoma cell line, but a different pattern was observed 24 hours after irradiation with 2 Gy dose with G2/M arrest only in radiosensitive cell line. The radiosensitivity and resistance to radiation-induced apoptosis in the radioresistant human breast carcinoma MDA-MB-231 cell line were similar to those observed in SKOV-3 cells. These data suggest that radiation-induced apoptosis and cell cycle alterations can predict radiosensitivity at least in some examined human malignant cells in vitro.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic. exonhun@savba.sk
authors.forename: L
authors.initials: L
authors.lastname: Hunáková
authors.forename: M
authors.initials: M
authors.lastname: Chorváth
authors.forename: J
authors.initials: J
authors.lastname: Duraj
authors.forename: Z
authors.initials: Z
authors.lastname: Bartosová
authors.forename: L
authors.initials: L
authors.lastname: Sevcíková
authors.forename: M
authors.initials: M
authors.lastname: Suliková
authors.forename: J
authors.initials: J
authors.lastname: Chovancová
authors.forename: J
authors.initials: J
authors.lastname: Sedlák
authors.forename: B
authors.initials: B
authors.lastname: Chorváth
authors.forename: E
authors.initials: E
authors.lastname: Boljesíková
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured / Q000528:radiation effects