Optimizing sensitivity of the human sperm motility assay for embryo toxicity testing.
The human sperm motility assay was used as a measure of quality control in the IVF laboratory. The effects of albumin supplementation and incubation time on the sensitivity of the human sperm motility assay were investigated. The assay was also compared with mouse embryo development. The human sperm motility assay and mouse embryo development assays were performed on 25 items commonly used in IVF laboratories. Sperm motility assay was conducted after 2, 4, 6, 8, 24 and 48 h incubation intervals under standard embryology conditions. A calculated sperm motility index value <0.75 was used to indicate sperm toxicity. It was found that optimum sensitivity (P < 0.01) of the human sperm motility assay was attained in the absence of albumin after 4, 8 and 48 h incubation periods. Items identified to be sperm toxic within 8 h by the human sperm motility assay were considered to be of clinical significance due to the close concordance of these results with mouse embryo development.
Claassens
O E
OE
Queensland Fertility Group, 225 Wickham Terrace, Brisbane 4000, Australia.
Wehr
J B
JB
Harrison
K L
KL
D000818:Animals; D005314:Embryonic and Fetal Development; D005307:Fertilization in Vitro / Q000009:adverse effects* / Q000295:instrumentation / Q000592:standards*; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011786:Quality Control; D012661:Semen / Q000502:physiology; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000494:pharmacology; D013076:Sperm Count; D013081:Sperm Motility; D013997:Time Factors
D012709:Serum Albumin