Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in pharmacokinetic study.
A specific method for the simultaneous determination of S-(+)Ibuprofen and R-(-)Ibuprofen enantiomers in human plasma is described. Adopting a high-performance liquid chromatographic (HPLC) system with spectrofluorometer detector, the compounds were extracted from plasma in alcohol medium and were separated on C18 column, using a solution of acetonitrile-water-acetic acid-triethylamine as mobile phase. The limit of quantitation was 0.1 microg/mL for both compounds. The method was validated by intra-day assays at three concentration levels and was used in a kinetic study in healthy volunteers. During the study we carried out inter-day assays to confirm the feasibility of the method.
Canaparo
R
R
Department of Anatomy, Pharmacology and Forensic Medicine, University of Torino, Torino, Italy. canaparo@medfarm.unito.it
Muntoni
E
E
Zara
G P
GP
Della Pepa
C
C
Berno
E
E
Costa
M
M
Eandi
M
M
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007052:Ibuprofen / Q000097:blood* / Q000493:pharmacokinetics*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen