title: Effect of dental plaque on the oxidative metabolism of normal neutrophils.
abstract: The purpose of this study was to investigate the interaction between dental plaque and the oxidative metabolism of blood (PB), crevicular (CR) and salivary (SAL) PMN. Data indicated that supragingival plaque induced an in vitro production of chemiluminescence by both PB and CR--PMN in a dose-dependent manner with a maximum activity after 30 min incubation. Comparison between PB, CR and SAL-PMNs indicated that 1) both CR and SAL-PMNs spontaneously produced large quantities of oxygen radicals, 2) CR and SAL-PMNs further produced oxygen radicals upon phorbol myristate acetate or opsonized-zymosan stimulation, and 3) SAL-PMN could not be further activated by supragingival plaque.
authors.affiliation: Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624 Institut Pasteur, Lille, France.
authors.forename: J A
authors.initials: JA
authors.lastname: Charon
authors.forename: F
authors.initials: F
authors.lastname: Joachim
authors.forename: C
authors.initials: C
authors.lastname: Champagne
authors.forename: G
authors.initials: G
authors.lastname: Torpier
authors.forename: A
authors.initials: A
authors.lastname: Capron
mesh_terms: D003773:Dental Plaque / Q000276:immunology*; D005883:Gingival Crevicular Fluid / Q000276:immunology; D006801:Humans; D008163:Luminescent Measurements; D008854:Microscopy, Electron; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst; D012463:Saliva / Q000276:immunology
chemical_list: D017382:Reactive Oxygen Species