Analysis of the sulfomycin component of alexomycin in animal feed by enhanced solvent extraction and supercritical fluid chromatography.
Enhanced solvent extraction (ESE) was used to isolate components of alexomycin from different types of animal feed samples. It was demonstrated that ESE was more economical as regards money and, for this particular matrix, twice as fast as supercritical fluid extraction (SFE) even though it was demonstrated that alexomycin can be extracted from feed quantitatively using both ESE and SFE. Supercritical fluid chromatography was used to separate alexomycin from other co-extractives of feed since liquid chromatography was unable to isolate the alexomycin for quantification purposes. Our results also showed that the concentration of alexomycin in an extruded sample was approximately half of the concentration which was originally added to the feed.
Ashraf-Khorassani
M
M
Durability, Inc., Corporate Research Center, Blacksburg, VA 24060, USA.
Taylor
L T
LT
Marr
J G
JG
D000821:Animal Feed / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000432:Methanol / Q000737:chemistry; D010455:Peptides; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D011437:Propylamines / Q000737:chemistry; D013997:Time Factors
D000900:Anti-Bacterial Agents; D010455:Peptides; D010456:Peptides, Cyclic; D011437:Propylamines; C413282:promothiocin; C049270:sulfomycin; C035263:2-propylamine; C102859:Alexomycin; D000432:Methanol