Expression of estrogen receptor-beta protein and mRNA in the cerebellum of the rat.
Estrogen has been shown to play a modulatory role in cerebellar neuronal signaling. Recent reports have also shown that estrogen receptor beta (ER-beta) mRNA is expressed in cerebellum. The purpose of the present study was to identify and map ER-beta protein expression, and to determine the identity of the major splice variants of ER-beta mRNA in the cerebellum. Polyclonal antibodies raised against the NH(3)- and COOH-termini of rat ER-beta were used for immunohistochemistry. Mapping of ER-beta immunoreactivity was compared with the distribution of ER-beta mRNA using in situ hybridization. We also determined, using RT-PCR, whether the ER-beta mRNA was variably spliced in cerebellum. Our results show that in all cases the distribution of ER-beta protein was identical to the distribution of ER-beta mRNA. Both Purkinje cells and scattered cells in the granule cell layer, perhaps golgi cells, robustly expressed ER-beta. Reverse transcription-polymerase chain reaction analysis showed that three splice variants in addition to wild type ER-beta are expressed in rat cerebellum. However, wild type ER-beta was the predominant form. These observations provide anatomical evidence that neurons in the cerebellum express ER-beta and thus may be targets of estrogen action.
Price
R H
RH
Department of Cell Biology, Neurobiology and Anatomy, Loyola University Chicago, IL 60153, Maywood, USA.
Handa
R J
RJ
D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D002531:Cerebellum / Q000378:metabolism*; D047629:Estrogen Receptor beta; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics*; D014158:Transcription, Genetic
D047629:Estrogen Receptor beta; D012333:RNA, Messenger; D011960:Receptors, Estrogen