Production of a heterologous endo-1,4-beta-xylanase in the yeast Pichia stipitis with an O(2)-regulated promoter.
The Cryptococcus albidus XLN-gene (encoding endo-1,4-beta-xylanase) was expressed in the yeast Pichia stipitis under the control of the PsADH2-promoter, which is activated under O(2) limitation. The resulting transformant produced endo-1,4-beta-xylanase after a shift to anoxic conditions. Endo-1,4-beta-xylanase production was enhanced by limited aeration after the shift.
Passoth
V
Applied Microbiology, Lund University, P.O. Box 124, 22100, Lund, Sweden
Hahn-Hägerdal
B