title: Mass spectrometric analysis of hydroxyproline glycans.
abstract: Mass spectrometric techniques are presented which allow one to analyze the sugar part bound to hydroxyproline in hydroxyproline-rich glycoproteins. The hydroxyproline (Hyp) glycans obtained by alkaline hydrolysis give abundant [M + Na](+) ions by electrospray ionization which after collision-induced dissociation (CID) yield inter alia [Hyp - H + Na](+). In mixtures a parent ion scan of this species will indicate the various molecular species which can then be analyzed by MS(n) after CID in an ion trap, where successive losses of the sugar units are observed. Methylation techniques allow one to distinguish between linear and branched isomeric structures.
authors.affiliation: Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, 50939 Cologne, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kilz
authors.forename: S
authors.initials: S
authors.lastname: Waffenschmidt
authors.forename: H
authors.initials: H
authors.lastname: Budzikiewicz
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D016825:Chlamydomonas reinhardtii / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D006909:Hydroxyproline / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D008745:Methylation; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D006909:Hydroxyproline