Cbfa1, an essential transcription factor for bone formation, is expressed in testis from the same promoter used in bone.
Cbfa1 is an essential transcription factor for bone formation, but its transcripts have also been detected in thymus and testis. To elucidate the expression of Cbfa1 in testis, we isolated Cbfa1 cDNA from mouse testis. First we examined the length of the transcripts expressed in mouse testis by Northern hybridization. Using a cDNA probe that can detect both Type-I and Type-II Cbfa1 (Type-I: originally reported as Pebp2alphaA by Ogawa et al.; Type-II: originally reported as til-1 by Stewart et al.), 1.8-kb transcripts were detected in testis, and larger transcripts (6.3 kb and 7.4 kb) in T-cell lines, thymus and bone. Using a Type-II specific probe (bone isoform-specific probe), surprisingly, 1.8-kb testis transcript(s) were detected as well as those (6.3 kb) found in bone. In addition, the transcription start site in mouse testis coincides with one of three start sites identified in bone. These results suggest that the testis transcript is generated from the same promoter of Type-II Cbfa1, which is thought to be active only in osteoblasts and some chondrocytes. Next, to define the difference in length of transcripts, we isolated the Cbfa1 cDNA from mouse testis. Sequence analysis of the cDNA showed that alternative splicing around exon 2 and poly-adenylation within exon 8 occurred in the testis isoform, which produce the smaller transcripts. These data revealed that testis Cbfa1 mRNA is transcribed from same promoter used in bones, but the post-transcriptional regulation is different between testis and skeletal tissues.
Ogawa
S
S
Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
Harada
H
H
Fujiwara
M
M
Tagashira
S
S
Katsumata
T
T
Takada
H
H
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001842:Bone and Bones / Q000378:metabolism*; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D011061:Poly A / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D012323:RNA Processing, Post-Transcriptional; D013601:T-Lymphocytes / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism; D014018:Tissue Distribution; D014157:Transcription Factors / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
D050659:Core Binding Factor Alpha 1 Subunit; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D014157:Transcription Factors; D011061:Poly A