Candida albicans triggers interleukin-6 and interleukin-8 responses by oral fibroblasts in vitro.
Oral candidiasis is the most frequent opportunistic infection associated with an immunocompromised host. Production of proinflammatory cytokines, such as interleukin-6 (IL-6) and IL-8, by host cells in response to Candida albicans can be expected to have a major impact in the activation of immune effector cells against the invading microorganism. Using a human cell--C. albicans coculture model system, we determined that this microorganism can trigger secretion of these potent chemoattractant and proinflammatory cytokines by oral mucosal fibroblasts. This response varied depending on the infecting strain and required fungal viability, germination of yeast into hyphae and mannose-mediated direct contact between the host cell and Candida. The secretion of proinflammatory cytokines by oral mucosal fibroblasts in response to C. albicans suggests that these cells have the potential to enhance the host defense against this organism in vivo. This may have important implications in controlling fungal overgrowth in the oral cavity.
Dongari-Bagtzoglou
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A
Columbia University School of Dental and Oral Surgery, New York, NY 10032, USA.
Wen
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K
Lamster
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IB
D002176:Candida albicans / Q000472:pathogenicity*; D002478:Cells, Cultured; D018920:Coculture Techniques; D005347:Fibroblasts / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D008358:Mannose / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology*; D018375:Neutrophil Activation
D015850:Interleukin-6; D016209:Interleukin-8; D007378:Interleukins; D008358:Mannose