Amperometric immunosensors based on protein A coupled polyaniline-perfluorosulfonated ionomer composite electrodes.
A very sensitive immunosensor based on polyaniline/ Nafion/protein A (PA/NF/PrA) composite electrodes has been developed for the amperometric immunoanalysis with urease-labeled immunoreagents. The use of urease conjugated goat anti-RIgG (GaRIgG-Ur) as the labeled antibody and urea as the substrate with an amperometric detection at -200 mV (vs Ag/AgCl) resulted in a dynamic range of 50-2000 ng mL-1 and a low detection limit of 10 ng/mL (64 pM) for the immunoanalysis of rabbit immunoglobulin G (RIgG). Because of the special affinity between protein A and RIgG, the PA/NF/PrA electrode can be regenerated repetitively by changing the pH of the buffer solutions. Characteristics of the PA/NF/PrA/RIgG immunosensor and optimal conditions for the competitive immunoanalysis of RIgG with FIA were studied.
Liu
C H
CH
Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
Liao
K T
KT
Huang
H J
HJ
D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry; D007202:Indicators and Reagents; D013205:Staphylococcal Protein A / Q000737:chemistry*
D000814:Aniline Compounds; D005465:Fluorocarbon Polymers; D007202:Indicators and Reagents; D013205:Staphylococcal Protein A; C040402:perfluorosulfonic acid