Regiospecific transglycolytic synthesis and structural characterization of 6-O-alpha-glucopyranosyl-glucopyranose (isomaltose).
The enzymatic synthesis of 6-O-alpha-glucopyranosyl-glucopyranose (isomaltose) was achieved. The regiospecific transglycosylation reaction was catalyzed by a crude preparation of alpha-D-glucosidase from Aspergillus niger, using p-nitrophenyl alpha-D-glucopyranose as the donor and glucopyranose as the acceptor. The yield of the reaction was 59% on a molar basis with respect to the donor. The structural identity of the product was fully determined by HPLC, HPAEC-PAD, ionspray mass spectrometry and (13)C NMR.
Vetere
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Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Via L. Giorgieri 1, Trieste, I-34127, Italy. vetere@bbcm.univ.trieste.it
Gamini
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Campa
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Paoletti
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D001234:Aspergillus niger / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D007534:Isomaltose / Q000138:chemical synthesis* / Q000737:chemistry* / Q000302:isolation & purification; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008956:Models, Chemical; D013997:Time Factors; D000520:alpha-Glucosidases / Q000737:chemistry
D007534:Isomaltose; D000520:alpha-Glucosidases