Plasma total homocysteine and other thiols analyzed by capillary electrophoresis/laser-induced fluorescence detection: comparison with two other methods.
We present a new analytical method for thiol quantification in plasma, based on the use of capillary electrophoresis (CE) and laser-induced fluorescence (LIF) to analyze 6-iodoacetamidofluorescein derivatives. Quantitative results of homocysteine, glutathione, cysteinylglycine, and cystationine are presented. A comparison of the quantitation of homocysteine in plasma, using high performance liquid chromatography/fluorescence detection and fluorescence polarization immunoassay is proposed. The results indicate that these techniques for plasma total homocysteine (tHcy) determination can be used interchangeably. The major advantage of CE-LIF is that it can quantitate the thiols in one run while keeping the price of consumables reasonable.
Caussé
E
E
Laboratoire de Biochimie/INSERM U466, Centre Hospitalier Rangueil, Toulouse, France.
Malatray
P
P
Calaf
R
R
Charpiot
P
P
Candito
M
M
Bayle
C
C
Valdiguié
P
P
Salvayre
R
R
Couderc
F
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005454:Fluorescence Polarization / Q000379:methods; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013438:Sulfhydryl Compounds / Q000097:blood*
D013438:Sulfhydryl Compounds; D006710:Homocysteine