Amino acid sequence of a nuclease (nuclease Le1) from Lentinus edodes.
The fruit bodies of Lentinus edodes produce two acid nucleases, nucleases Le1 and Le3, both of which are thought to be candidates for the enzymes producing a tasty substance, 5'-GMP. To obtain the basic information on the mechanism of production of 5'-GMP, and structure-function relationship of these nucleases, the primary structure of nuclease Le1 was estimated by both protein chemistry and gene cloning. Nuclease Le1 is a glycoprotein and consists of 290 amino acid residues, and about 2 and 6 residues of hexosamine and neutral sugar, respectively. The nucleotide sequence of cDNA and genomic DNA encoding nuclease Le1 indicated the presence of 20 amino acid residues of a signal peptide. Nuclease Le1 has 115 and 108 residues of identical amino acid residues with nucleases P1 and S, respectively. The amino acid residues concerning the coordination with Zn2+ in nuclease P1 are all conserved in nuclease Le1. Nuclease Le1 contains 8 half-cystine residues and 4 of them are located at the same places as those of nucleases P1 and S.
Kobayashi
H
H
Department of Microbiology, College of Pharmacy, Nihon University, Funabashi, Chiba, Japan.
Kumagai
F
F
Itagaki
T
T
Inokuchi
N
N
Koyama
T
T
Iwama
M
M
Ohgi
K
K
Irie
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D008969:Molecular Sequence Data; D009708:Nucleotidases / Q000737:chemistry*; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000235:genetics
D018076:DNA, Complementary; D012260:Ribonucleases; D009708:Nucleotidases; C040470:3'-nucleotidase