Use of spectrophotometric reading for in vitro antifungal susceptibility testing of Aspergillus spp.
A comparative study of visual and spectrophotometric MIC endpoint determinations for antifungal susceptibility testing of Aspergillus species was performed. A broth microdilution method adapted from the National Committee for Clinical Laboratory Standards (NCCLS) was used for susceptibility testing of 180 clinical isolates of Aspergillus species against amphotericin B and itraconazole. MICs were determined visually and spectrophotometrically at 490 nm after 24, 48, and 72 h of incubation, and MIC pairs were compared. The agreement between the two methods was 99% for amphotericin B and ranged from 95 to 98% for itraconazole. It is concluded that spectrophotometric MIC endpoint determination is a valuable alternative to the visual reference method for susceptibility testing of Aspergillus species.
Dannaoui
E
E
Laboratoire de Parasitologie, Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I, France. edanna@rockefeller.univ-lyon1.fr
Persat
F
F
Monier
M F
MF
Borel
E
E
Piens
M A
MA
Picot
S
S
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects* / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009172:Mycology / Q000379:methods; D013053:Spectrophotometry / Q000379:methods*
D000935:Antifungal Agents; D017964:Itraconazole; D000666:Amphotericin B