Development of a sensitive ELISA for the determination of fumonisin B(1) in cereals.
Monoclonal fumonisin B(1) antibodies with high titer were raised by using FB(1)-glutaraldehyde-keyhole limpet hemocyanin immunogen prepared by a short cross-linker reagent (glutaraldehyde). Mean cross-reactivities of the selected monoclonal antibody for FB(1), FB(2), and FB(3) were 100, 91.8, and 209%, respectively; no reactivity was found with hydrolyzed fumonisin. A direct, competitive enzyme-linked immunosorbent assay for the quantitative determination of FB(1) in cereals has been developed with this antibody. Fifty percent acetonitrile-based solvent with some additives was used for extraction of cereals, and the diluted extracts were used without cleanup in the test. The mean within-assay and interassay coefficients of variation for the standard curve were <10%. The measuring range of this test is 10-500 ng/g, with a detection limit of 7.6 ng/g FB(1). The toxin recovery from cereals infected with 50-200 ng/g of FB(1) varied between 61 and 84%. According to the comparable results of naturally infected maize samples, this test proved to be suitable for the rapid screening of food and feed samples for the presence of FBs.
Barna-Vetró
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I
Agricultural Biotechnology Center, P.O. Box 411, H-2101 Gödöllo, Hungary. barna@abc.hu
Szabó
E
E
Fazekas
B
B
Solti
L
L
D002264:Carboxylic Acids / Q000032:analysis*; D003432:Cross-Linking Reagents; D002523:Edible Grain / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D037341:Fumonisins; D005976:Glutaral; D008956:Models, Chemical
D002264:Carboxylic Acids; D003432:Cross-Linking Reagents; D037341:Fumonisins; C056933:fumonisin B1; D005976:Glutaral