title: Structural organization of the RNA polymerase-promoter open complex.
abstract: We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RPo). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of RPo, and permit analysis of effects of a transcriptional activator on the structure of RPo.
authors.affiliation: Howard Hughes Medical Institute, Department of Chemistry, Rutgers University, Piscataway, New Jersey 08854, USA.
authors.forename: N
authors.initials: N
authors.lastname: Naryshkin
authors.forename: A
authors.initials: A
authors.lastname: Revyakin
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: V
authors.initials: V
authors.lastname: Mekler
authors.forename: R H
authors.initials: RH
authors.lastname: Ebright
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011487:Protein Conformation; D014158:Transcription, Genetic
chemical_list: D004247:DNA; D012321:DNA-Directed RNA Polymerases