title: [Enzymatic resolution of racemic naproxen in a low aqueous-organic biphase system].
abstract: A stereoselective hydrolysis of the racemic naproxen methyl ester by immobilized lipase from Candida rugosa in a low aqueous-organic biphase system was studied. Support polar, water content, the logP value of organic phase and product inhibition effected the activity of immobilized enzyme. According to these reaction conditions, a low aqueous-organic biphase system for the continuous production of (S)-(+)-Naproxen was developed. The reaction was carried out in a continuous-flow closed-loop 50 mL stirred bioreactor packed with YWG-C6H5, a poorly polar synthetic support on which the lipase had been immobilized by adsorption. The aqueous phase was permanently remained in the reactor associated with the immobilized enzyme particles; the organic phase containing substrate was pumped through this reactor and emerged with the products. The continous-flow stirred bioreactor containing 75 mg lipase was allowed to operate continuously for 60 days at 30 degrees C with a 25% loss of activity, 900 mg of (S)-(+)-Naproxen (eep 95%) were producted.
authors.affiliation: OSSO, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences.
authors.forename: J Y
authors.initials: JY
authors.lastname: Xin
authors.forename: S B
authors.initials: SB
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: L L
authors.initials: LL
authors.lastname: Wang
authors.forename: R N
authors.initials: RN
authors.lastname: Shen
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism; D009288:Naproxen / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004800:Enzymes, Immobilized; D009288:Naproxen; D008049:Lipase