Anaerobic accumulation of amino acids in rice roots: role of the glutamine synthetase/glutamate synthase cycle.
Accumulation of amino acids was studied in rice roots of 3-day-old seedlings subjected for 48h to anaerobic conditions. Alanine and Gaba were the main amino acids accumulated under anoxia. Their synthesis was strongly inhibited by MSX and AZA, inhibitors of glutamine synthetase and glutamate synthase. These activities increased after 8h of anaerobic treatment and, by immunoprecipitation of 35S-labeled proteins, it was shown that glutamine synthetase and ferredoxin-dependent glutamate synthase were synthesized during the treatment. These findings indicate that the glutamine synthetase/glutamate synthase cycle play an important role in anaerobic amino acid accumulation.
Reggiani
R
R
Istituto Biosintesi Vegetali, CNR, Milano, Italy.
Nebuloni
M
M
Mattana
M
M
Brambilla
I
I
D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000935:Antifungal Agents / Q000494:pharmacology; D001377:Azaserine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005288:Ferredoxins / Q000378:metabolism; D005970:Glutamate Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008717:Methionine Sulfoximine / Q000494:pharmacology; D009243:NAD / Q000378:metabolism; D012275:Oryza / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D011233:Precipitin Tests; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000596:Amino Acids; D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D005288:Ferredoxins; D009243:NAD; D008717:Methionine Sulfoximine; D005680:gamma-Aminobutyric Acid; D001377:Azaserine; D005970:Glutamate Synthase; D005974:Glutamate-Ammonia Ligase; D000409:Alanine; D010100:Oxygen