[Changes in the EPR spectra of the nitrosyl complexes of blood proteins in the low-intensity whole-body irradiation of mice].
After NO adding to mice blood and isolated erythrocytes ESR signal of nitrozyl complex HbNO (g = 2.07, g = 1.98) and NO-induced MetNg (g = 6.0) were registered. It was shown that the intensity of ESR spectra of these complexes increased after radiation of mice with a dose of 0.06, 0.6 and 5.4 cGy. Low-dose irradiation (0.6 and 0.06 cGy) caused the change in the form of ESR spectra of HbNO (g = 2.07), which is indicative of the shift from T-structure to R-structure and of the preferred formation of R-conformations of oxyhemoglobin in blood. It was found that dependence of NO-induced MetHb signal on irradiation dose is bimodal that may be connected with nonlinear response of the cells to irradiation and retarded adaptive response after radiation with low doses.
Mil'
E M
EM
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow. Emil@chat.ru
Kasparov
V V
VV
Biniukov
V I
VI
Tabatchikova
N V
NV
Borisova
O A
OA
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006454:Hemoglobins / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D008706:Methemoglobin / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009569:Nitric Oxide / Q000494:pharmacology; D013997:Time Factors; D014916:Whole-Body Irradiation
D006454:Hemoglobins; C008657:nitrosyl hemoglobin; D009569:Nitric Oxide; D008706:Methemoglobin