title: A simple method for measurement of CD14weak CD16strong monocytes in peripheral blood.
abstract: Mounting evidence for the clinical significance of the CD 14weak CD16strong monocyte subpopulation in peripheral blood induced the demand for an efficient method for its determination. We propose a simple, fast, no-wash flow cytometric method using fluorescence-labelled anti-CD14, anti-CD16, and anti-HLA-DR antibodies and ammonium chloride-based erythrocyte lysis. This type of analysis can be performed on a standard three-colour flow cytometer. The method avoids interference by NK-cells and neutrophil granulocytes without defining monocytes by stringent light scatter criteria that might lead to a loss of CD14weak CD16strong monocytes. It, therefore, offers high reliability and accuracy. Its performance recommends the method to be used for routine clinical measurements of CD14weak CD16strong monocytes.
authors.affiliation: Central Laboratory, Wilhelminenspital, Vienna, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Hübl
authors.forename: L H
authors.initials: LH
authors.lastname: Ziegler-Heitbrock
authors.forename: P M
authors.initials: PM
authors.lastname: Bayer
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D017452:Receptors, IgG / Q000032:analysis / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG