courtless, the Drosophila UBC7 homolog, is involved in male courtship behavior and spermatogenesis.
The courtless (col) mutation disrupts early steps of courtship behavior in Drosophila males, as well as the development of their sperm. Most of the homozygous col/col males (78%) do not court at all. Only 5% perform the entire ritual and copulate, yet these matings produce no progeny. The col gene maps to polytene chromosome band 47D. It encodes two proteins that differ in their carboxy termini and are the Drosophila homologs of the yeast ubiquitin-conjugating enzyme UBC7. The col mutation is caused by an insertion of a P element into the 3' UTR of the gene, which probably disrupts translational regulatory elements. As a consequence, the homozygous mutants exhibit a six- to sevenfold increase in the level of the COL protein. The col product is essential, and deletions that remove the col gene are lethal. During embryonic development col is expressed primarily in the CNS. Our results implicate the ubiquitin-mediated system in the development and function of the nervous system and in meiosis during spermatogenesis.
Orgad
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Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Tel-Aviv 69978, Israel.
Rosenfeld
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Greenspan
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Segal
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D000483:Alleles; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D003001:Cloning, Molecular; D003380:Courtship; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins; D005260:Female; D015870:Gene Expression; D006720:Homozygote; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics*; D008025:Ligases / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010453:Peptide Synthases; D020161:Physical Chromosome Mapping; D017386:Sequence Homology, Amino Acid; D012726:Sexual Behavior, Animal / Q000502:physiology; D013091:Spermatogenesis / Q000235:genetics*; D014162:Transfection; D044763:Ubiquitin-Conjugating Enzymes; D014452:Ubiquitins / Q000378:metabolism
D029721:Drosophila Proteins; D019476:Insect Proteins; D014452:Ubiquitins; C079437:UBE2L3 protein, human; C412168:Ubc7 protein, Drosophila; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases; D010453:Peptide Synthases