Rapid simultaneous determination of glucagon and insulin by capillary electrophoresis immunoassays.
A rapid capillary electrophoresis (CE) with laser-induced fluorescence (LIF) competitive immunoassay has been developed for the determination of glucagon in biological mixtures. In the assay, fluorescein-conjugated glucagon is mixed with the sample followed by addition of anti-glucagon. Free and antibody-bound, tagged glucagon could be separated in 3 s using CE to obtain quantitative determination of glucagon with a concentration detection limit of 760 pM. The assay was combined with a previously developed competitive immunoassay for insulin to produce a simultaneous immunoassay for both peptides. The method was used to determine glucagon content of islets of Langerhans.
German
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Department of Chemistry, University of Florida, Gainesville 32611-7200, USA.
Kennedy
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D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D017022:Flow Injection Analysis; D005934:Glucagon / Q000032:analysis*; D007118:Immunoassay; D007328:Insulin / Q000032:analysis*; D051379:Mice
D007328:Insulin; D005934:Glucagon