title: In vitro micronucleus assay with Chinese hamster V79 cells - results of a collaborative study with in situ exposure to 26 chemical substances.
abstract: A collaborative study with 10 participating laboratories was conducted to evaluate a test protocol for the performance of the in vitro micronucleus (MN) test using the V79 cell line with one treatment and one sampling time only. A total of 26 coded substances were tested in this study for MN-inducing properties. Three substances were tested by all 10 laboratories and 23 substances were tested by three or four laboratories in parallel. Six aneugenic, 7 clastogenic and 6 non-genotoxic chemicals were uniformly recognised as such by all laboratories. Three chemicals were tested uniformly negative by three laboratories although also clastogenic properties have been reported for these substances. Another set of three clastogenic substances showed inconsistent results and one non-clastogenic substance was found to be positive by one out of three laboratories. Within the study, the applicability of the determination of a proliferation index (PI) as an internal cytotoxicity parameter in comparison with the determination of the mitotic index (MI) was also evaluated. Both parameters were found to be useful for the interpretation of the MN test result with regard to the control of cell cycle kinetics and the mode of action for MN induction. The MN test in vitro was found to be easy to perform and its results were mainly in accordance with results from chromosomal aberration tests in vitro.
authors.affiliation: Federal Institute for Health Protection of Consumers and Veterinary Medicine, BgVV, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: von der Hude
authors.forename: S
authors.initials: S
authors.lastname: Kalweit
authors.forename: G
authors.initials: G
authors.lastname: Engelhardt
authors.forename: S
authors.initials: S
authors.lastname: McKiernan
authors.forename: P
authors.initials: P
authors.lastname: Kasper
authors.forename: R
authors.initials: R
authors.lastname: Slacik-Erben
authors.forename: H G
authors.initials: HG
authors.lastname: Miltenburger
authors.forename: N
authors.initials: N
authors.lastname: Honarvar
authors.forename: R
authors.initials: R
authors.lastname: Fahrig
authors.forename: B
authors.initials: B
authors.lastname: Görlitz
authors.forename: S
authors.initials: S
authors.lastname: Albertini
authors.forename: S
authors.initials: S
authors.lastname: Kirchner
authors.forename: D
authors.initials: D
authors.lastname: Utesch
authors.forename: F
authors.initials: F
authors.lastname: Pötter-Locher
authors.forename: H
authors.initials: H
authors.lastname: Stopper
authors.forename: S
authors.initials: S
authors.lastname: Madle
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D015162:Micronucleus Tests; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D015203:Reproducibility of Results
chemical_list: D000970:Antineoplastic Agents; D009153:Mutagens