title: Purification and molecular cloning of chemosensory proteins from Bombyx mori.
abstract: Soluble low molecular weight acidic proteins are suspected to transport stimulus molecules to the sensory neurons within insect sensilla. From the antennae of Bombyx mori, we have purified and sequenced a protein (BmorCSP1) bearing sequence similarity to a class of soluble chemosensory proteins recently discovered in several orders of insects. Based on its N-terminal sequence, the cDNA encoding this protein has been amplified and cloned. Differential screening of a B. mori antennal cDNA library led to the identification of a second gene encoding a related protein (BmorCSP2), sharing 35-40% identity to BmorCSP1 and chemosensory proteins from other species. The predicted secondary structures of moth's, chemosensory proteins comprise alpha-helical foldings at conserved positions and a reduced hydrophobicity with respect to this novel family of chemosensory proteins.
authors.affiliation: Institut für Physiologie, Universität Hohenheim, Stuttgart, Germany. jfp@uni-hohenheim.de
authors.forename: J F
authors.initials: JF
authors.lastname: Picimbon
authors.forename: K
authors.initials: K
authors.lastname: Dietrich
authors.forename: S
authors.initials: S
authors.lastname: Angeli
authors.forename: A
authors.initials: A
authors.lastname: Scaloni
authors.forename: J
authors.initials: J
authors.lastname: Krieger
authors.forename: H
authors.initials: H
authors.lastname: Breer
authors.forename: P
authors.initials: P
authors.lastname: Pelosi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009475:Neurons, Afferent / Q000737:chemistry*; D014018:Tissue Distribution; D014020:Tissue Extracts
chemical_list: C412286:CSP1 protein, Bombyx mori; C412285:CSP2 protein, Bombyx mori; D019476:Insect Proteins; D014020:Tissue Extracts