Detection of Mycobacterium tuberculosis complex in lymph nodes of wild boar (Sus scrofa) by a target-amplified test system.
A detailed macroscopic and histological description is given of tuberculous-like lesions in mandibular lymph nodes obtained from wild boar (Sus scrofa) collected in Italy during the 1995/1996 hunting season, as well as a correlation with results obtained from a Mycobacterium tuberculosis complex target amplified test system. According to macroscopic appearance, lesions were largely represented by caseous-necrotic-calcified granulomata (n = 272/285; 95.4%), while histological investigations showed mainly necrotic-calcified (n = 82/218; 37.6%) and fibronecrotic-calcified (n = 81/218; 37.2%) lesions. When tested with a target rRNA amplification/hybridization technique to detect M. tuberculosis complex, 112 (43.6%) samples out of 275 tested gave positive results.
Bollo
E
E
Università degli Studi di Torino, Dipartimento di Patologia Animale, Italy.
Ferroglio
E
E
Dini
V
V
Mignone
W
W
Biolatti
B
B
Rossi
L
L
D000818:Animals; D000835:Animals, Wild; D008198:Lymph Nodes / Q000382:microbiology* / Q000473:pathology; D008334:Mandible; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012335:RNA, Ribosomal / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology*; D014376:Tuberculosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*
D012335:RNA, Ribosomal