title: Particle size determination of a three-component suspension using a laser-scattering particle size distribution analyzer.
abstract: In this study, a rapid and accurate particle size determination method using a light-scattering particle size analyzer was developed to measure the particle size and size distribution of a suspension containing three solid components: clotrimazole, triamcinolone, and sarafloxacin, which have different refractive indices. To ensure that data represent the size distribution of the primary particles of the suspension, the optimal sonication prior to and during measurement was determined. It was found that the results obtained using the average relative refractive index (RRI) of the three components agreed with the results obtained using three individual RRIs. In addition, the results from two analysts demonstrated good reproducibility of this method. The size distribution data of the suspension were also compared to those of the bulk drugs. The results showed that the median particle size of this three-component suspension is relatively close to that of clotrimazole, which accounts for 80% of solid particles in the suspension. Furthermore, the results obtained using the light-scattering technique were comparable to those obtained using a polarized light microscope equipped with an image analyzer, indicating acceptable accuracy of this technique.
authors.affiliation: Abbott Laboratories, Department 97D, Abbott Park, IL 60064-6075, USA.
authors.forename: S
authors.initials: S
authors.lastname: Toongsuwan
authors.forename: H C
authors.initials: HC
authors.lastname: Chang
authors.forename: L C
authors.initials: LC
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Stephens
authors.forename: H
authors.initials: H
authors.lastname: Plichta-Mahmoud
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000737:chemistry; D003022:Clotrimazole / Q000737:chemistry; D024841:Fluoroquinolones; D007834:Lasers; D010316:Particle Size; D012031:Refractometry / Q000379:methods*; D013535:Suspensions / Q000737:chemistry*; D014221:Triamcinolone / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D013535:Suspensions; D014221:Triamcinolone; D002939:Ciprofloxacin; D003022:Clotrimazole; C047224:sarafloxacin