Apoptosis induced by the flavonoid from lemon fruit (Citrus limon BURM. f.) and its metabolites in HL-60 cells.
The flavonoid from lemon fruit (Citrus limon BURM. f.) and its metabolites, particularly eriodictyol, 3,4-dihydroxyhydrocinnamic acid, and phloroglucinol had the function of DNA fragmentation in HL-60 cells when analyzed by flow cytometry. An apoptotic DNA ladder and chromatin condensation were observed in HL-60 cells when treated with these compounds. The caspase inhibitor prevented DNA fragmentation. These compounds are anticipated to be useful for medical purposes.
Ogata
S
S
Department of Life Science, Faculty of Bioresources, Mie University, Tsu, Japan.
Miyake
Y
Y
Yamamoto
K
K
Okumura
K
K
Taguchi
H
H
D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002109:Caffeic Acids / Q000494:pharmacology; D061945:Caspase Inhibitors; D048429:Cell Size; D002957:Citrus / Q000737:chemistry*; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D044950:Flavanones; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D018922:HL-60 Cells; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D010696:Phloroglucinol / Q000494:pharmacology
D002109:Caffeic Acids; D061945:Caspase Inhibitors; D044950:Flavanones; D005419:Flavonoids; C114706:eriocitrin; D010696:Phloroglucinol; D006569:Hesperidin; C007619:eriodictyol; C040048:caffeic acid