Amplified restriction fragment length polymorphism in parasite genetics.
The amplified restriction fragment length polymorphism (AFLP) technique is a relatively new method for the analysis of polymorphism that has not yet been widely used in parasitology. In this article, Dan Masiga, Andy Tait and Mike Turner provide a brief introduction to AFLP and illustrate how it can be used in the investigation of marker inheritance in genetic crosses and in the analysis of polymorphism of field populations. They also briefly highlight the strengths and weaknesses of AFLP in comparison with other methods for detecting polymorphism and conclude that AFLP is a very useful addition to the range of techniques available.
Masiga
D K
DK
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, UK G12 8QQ.
Tait
A
A
Turner
C M
CM
D000818:Animals; D003433:Crosses, Genetic; D006801:Humans; D010271:Parasites / Q000235:genetics*; D010272:Parasitic Diseases / Q000453:epidemiology / Q000469:parasitology; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length