title: Postischemic recovery of heart metabolism and function: role of mitochondrial fatty acid transfer.
abstract: Postischemic recovery of contractile function is better in hearts from fasted rats than in hearts from fed rats. In this study, we examined whether feeding-induced inhibition of palmitate oxidation at the level of carnitine palmitoyl transferase I is involved in the mechanism underlying impaired recovery of contractile function. Hearts isolated from fasted or fed rats were submitted to no-flow ischemia followed by reperfusion with buffer containing 8 mM glucose and either 0.4 mM palmitate or 0.8 mM octanoate. During reperfusion, oxidation of palmitate was higher after fasting than after feeding, whereas oxidation of octanoate was not influenced by the nutritional state. In the presence of palmitate, recovery of left ventricular developed pressure was better in hearts from fasted rats. Substitution of octanoate for palmitate during reperfusion enhanced recovery of left ventricular developed pressure in hearts from fed rats. However, the chain length of the fatty acid did not influence diastolic contracture. The results suggest that nutritional variation of mitochondrial fatty acid transfer may influence postischemic recovery of contractile function.
authors.affiliation: Cardiology Center, University Hospital of Geneva, CH-1211 Geneva 14, Switzerland. christophe.montessuit@hcuge.ch
authors.forename: C
authors.initials: C
authors.lastname: Montessuit
authors.forename: I
authors.initials: I
authors.lastname: Papageorgiou
authors.forename: I
authors.initials: I
authors.lastname: Tardy-Cantalupi
authors.forename: N
authors.initials: N
authors.lastname: Rosenblatt-Velin
authors.forename: R
authors.initials: R
authors.lastname: Lerch
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002210:Caprylates / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D004435:Eating / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D005215:Fasting / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D009200:Myocardial Contraction / Q000502:physiology; D017202:Myocardial Ischemia / Q000378:metabolism*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D010168:Palmitates / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D016277:Ventricular Function, Left / Q000502:physiology*
chemical_list: D002210:Caprylates; D002250:Carbon Radioisotopes; D010168:Palmitates; D002334:Carnitine O-Palmitoyltransferase; D003402:Creatine Kinase; D005947:Glucose; C031492:octanoic acid