[Investigation of fetal cells isolated from maternal blood by different methods].
This paper presents the results of investigation of the isolated from maternal blood by 4 different methods according to the optimized protocols. All women had male fetuses. The mononuclear cells with fetal erythroblasts were preisolated by using density-gradient centrifugation of the maternal blood in the Ficoll solution. Fetal cells were detected by FISH for Y-chromosomal sequences. The fetal cells were 2.1% by fluorescence activated cell sorting (FACS); 3.8% by magnetic field sorting, and 2.6% by two-stage density gradient precipitation. The fetal lymphocytes were investigated through air-cultivation of peripheral maternal lymphocytes. Their proportion was 3.9% in the culture samples. The findings lead to the conclusion that the new non-invasive approach is useful for prenatal diagnosis of chromosomal aneuploidies.
Zolotukhina
T V
TV
Shilova
N V
NV
Zamulaeva
I A
IA
Saenko
A S
AS
D000782:Aneuploidy; D002469:Cell Separation / Q000379:methods*; D004900:Erythroblasts / Q000166:cytology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D012729:Sex Chromosome Aberrations / Q000097:blood / Q000175:diagnosis / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics*