title: Change of Th0 to Th1 cell-cytokine profile following tuberculosis chemotherapy.
abstract: T cells mediate protection against tuberculosis, but little is known about their role during chemotherapy of patients with active disease. Here we examined the cytokine profile of CD4 T cells before and after four months of chemotherapy in six initial skin test anergic cases. Purified protein derivative (PPD) and 16-kDa antigen-reactive CD4 T-cell clones prior to therapy resided mostly in disease-associated body fluids and were of the Th0 (interferon (IFN)-gamma + interleukin (IL)-4) secreting profile. In contrast, the majority of postchemotherapy CD4 T-cell clones originated from blood and were of the IFN-gamma secreting Th1 type. However, the recognition of several peptides derived from the 16-kDa antigen was not significantly different between the Th1 and Th0 clones. We conclude that chemotherapy shifts CD4 T cells from the affected body fluids to the blood circulation, accompanied by a change from Th0 to Th1 cytokine profile.
authors.affiliation: Section of Pathology, Department of Biopathology, University of Palermo, 90134 Palermo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Dieli
authors.forename: M
authors.initials: M
authors.lastname: Singh
authors.forename: R
authors.initials: R
authors.lastname: Spallek
authors.forename: A
authors.initials: A
authors.lastname: Romano
authors.forename: L
authors.initials: L
authors.lastname: Titone
authors.forename: G
authors.initials: G
authors.lastname: Sireci
authors.forename: G
authors.initials: G
authors.lastname: Friscia
authors.forename: C
authors.initials: C
authors.lastname: Di Sano
authors.forename: D
authors.initials: D
authors.lastname: Santini
authors.forename: A
authors.initials: A
authors.lastname: Salerno
authors.forename: J
authors.initials: J
authors.lastname: Ivanyi
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003459:Crystallins / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D015847:Interleukin-4 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D014373:Tuberculin / Q000276:immunology; D014376:Tuberculosis / Q000188:drug therapy / Q000276:immunology*
chemical_list: D003459:Crystallins; D014373:Tuberculin; D015847:Interleukin-4; D007371:Interferon-gamma