Separation and detection techniques for peptides and proteins in stability research and bioanalysis.
In this paper, a brief overview of the most commonly used methods for the separation and analysis of peptides and proteins in stability and bioanalysis studies is presented. To investigate the physical stability of peptides and proteins, size-exclusion chromatography and electrophoretic separation techniques are being used, apart from several other methods. To determine the chemical stability of these compounds, separation systems are also important, with informative detection modes, such as various spectroscopic detections, electrochemical detection and mass spectrometric detection. For the bioanalysis of peptides, separation is the most important factor, while the detection must be done at the highest possible level of sensitivity.
Underberg
W J
WJ
Department of Pharmaceutical Analysis and Toxicology, Faculty of Pharmacy, Utrecht University, The Netherlands. w.j.m.underberg@far.ruu.nl
Hoitink
M A
MA
Reubsaet
J L
JL
Waterval
J C
JC
D000595:Amino Acid Sequence; D002850:Chromatography, Gel / Q000379:methods; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
D010455:Peptides; D011506:Proteins