Cloning and characterization of rat casein kinase 1epsilon.
Genes differentially expressed in the subjective day and night in the rat suprachiasmatic nucleus (SCN) were surveyed by differential display. A gene homologous to human casein kinase 1epsilon (CK1epsilon) was isolated, which initially appeared to be expressed in the suprachiasmatic nucleus (SCN) in a circadian manner. We here describe the cDNA cloning of the rat CK1epsilon and characterization of the protein products. The rCK1epsilon is predominantly expressed in the brain including the SCN, binds and phosphorylates mPer1, mPer2, and mPer3 in vitro, and translocates mPer1 and mPer3, but not mPer2, to the cell nucleus depending on its kinase activity when coexpressed with these Per proteins in COS-7 cells.
Takano
A
A
Division of Protein Metabolism, Institute for Protein Research, Osaka University, 3-2 Yamado-Oka, Suita, Osaka, Japan. atsuko@protein.osaka-u.ac.jp
Shimizu
K
K
Kani
S
S
Buijs
R M
RM
Okada
M
M
Nagai
K
K
D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001683:Biological Clocks; D001692:Biological Transport; D019556:COS Cells; D047388:Casein Kinases; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000378:metabolism; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D056950:Period Circadian Proteins; D010766:Phosphorylation; D011485:Protein Binding; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013493:Suprachiasmatic Nucleus / Q000378:metabolism; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D007527:Isoenzymes; D009687:Nuclear Proteins; C539022:PER1 protein, human; C421902:PER2 protein, human; C539023:Per1 protein, mouse; C539024:Per1 protein, rat; C513312:Per2 protein, mouse; C513311:Per2 protein, rat; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D011494:Protein Kinases; D047388:Casein Kinases