Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT).
Truett
G E
GE
Developmental Genetics Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA. truettge@pbrc.edu
Heeger
P
P
Mynatt
R L
RL
Truett
A A
AA
Walker
J A
JA
Warman
M L
ML
D000375:Aging; D000818:Animals; D002021:Buffers; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D003848:Deoxyguanine Nucleotides; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015636:Magnesium Chloride; D051379:Mice; D016133:Polymerase Chain Reaction; D012972:Sodium Hydroxide; D012995:Solubility; D019914:Taq Polymerase; D013942:Thymine Nucleotides
D002021:Buffers; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D003848:Deoxyguanine Nucleotides; D007202:Indicators and Reagents; D013942:Thymine Nucleotides; D015636:Magnesium Chloride; C024107:2'-deoxycytidine 5'-triphosphate; D012972:Sodium Hydroxide; C029603:deoxyguanosine triphosphate; D004247:DNA; D019914:Taq Polymerase; C026600:2'-deoxyadenosine triphosphate; C024157:thymidine 5'-triphosphate