title: A rapid and accurate closed-tube immunoassay for platelets on an automated hematology analyzer.
abstract: Accurate and precise platelet counts are important for patients with severe thrombocytopenia or who are receiving chemotherapy. We developed a novel flow cytometric analysis of platelets that may be particularly valuable for assessing the necessity for platelet transfusions. This ImmunoPlt (CD61) assay is based in part on CD61 monoclonal antibody labeling and has been automated and implemented on the CELL-DYN 4000 hematology analyzer. It is well suited for thrombocytopenic specimens, since it reduces interference by nonplatelet particles. It takes less than 5 minutes from closed-tube aspiration to report. Data for more than 350 thrombocytopenic specimens demonstrate that the ImmunoPlt (CD61) assay is more accurate than the optical scatter or the impedance count for specimens with platelet counts between 1 and 60 x 10(3)/microL (1 and 60 x 10(9)/L). The ImmunoPlt (CD61) assay is more precise than the optical scatter or the impedance count for specimens with platelet counts between 1 and 50 x 10(3)/microL (1 and 50 x 10(9)/L).
authors.affiliation: Research & Development Department, Abbott Diagnostics Division, Santa Clara, CA 95054, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Gill
authors.forename: K A
authors.initials: KA
authors.lastname: Davis
authors.forename: W J
authors.initials: WJ
authors.lastname: Cowart
authors.forename: F U
authors.initials: FU
authors.lastname: Nepacena
authors.forename: Y R
authors.initials: YR
authors.lastname: Kim
mesh_terms: D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D016477:Artifacts; D001331:Automation; D001792:Blood Platelets / Q000276:immunology* / Q000473:pathology; D004355:Drug Stability; D006405:Hematology / Q000295:instrumentation*; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards*; D039661:Integrin beta3; D008858:Microscopy, Phase-Contrast; D010976:Platelet Count; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039661:Integrin beta3; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; D010980:Platelet Membrane Glycoproteins