Density-dependent induction of TNF-alpha release from human monocytes by immobilized P-selectin.
P-selectin purified from human platelets, when immobilized on a solid surface, induced monocytes to release tumor necrosis factor-alpha (TNF-alpha). The induction of TNF-alpha release was dependent on the concentration of P-selectin used for the immobilization, and the maximal stimulation was observed when the plate was coated with 0.3 microgram/ml of P-selectin. Use of either a higher or a lower concentration of P-selectin for the plate-coating was found to elicit less TNF-alpha release, although the higher concentration of P-selectin caused a stronger adhesion of HL-60 leukemic cells. The expression of mRNA for TNF-alpha roughly paralleled the TNF-alpha secretion, as assessed by RT-PCR. These results indicate that monocytes are activated by immobilized P-selectin in a density-dependent manner.
Koike
J
J
Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
Nagata
K
K
Kudo
S
S
Tsuji
T
T
Irimura
T
T
D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D019007:P-Selectin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D019007:P-Selectin; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha