High-performance liquid chromatographic analysis of saponin compounds in Bupleurum falcatum.
A mixture of saponin compounds (saikosaponin c, a, and d) in the 70% ethanol extract of a powdered sample of Bupleuri radix are analyzed by an Inertsil ODS-3 C(18) column at a flow rate of 1.0 mL/min and detection wavelength of 203 nm. Well resolved chromatograms of saikosaponin c, a, and d are obtained with a gradient elution of acetonitrile-water from 40:60 (v/v) to 50:50 (v/v). The total time required for a single analysis is approximately 20 min. Calibration curves for saikosaponin c, a, and d are linear up to 2.5 mg/mL. The coefficient of variability values for saikosaponins in the extract are below 4%, and the recoveries for saikosaponin c, a, and d are 95.2 +/- 1.1, 96.5 +/- 0.9, and 96.2 +/- 1.0%, respectively. The changes in saikosaponin contents for a two-year growth of Bupleurum falcatum are measured by the established high-performance liquid chromatography method.
Park
I S
IS
Korea Food Research Institute, Backhyun-dong, Songnam-si, Republic of Korea.
Kang
E M
EM
Kim
N
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019684:Magnoliopsida / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D012502:Sapogenins / Q000032:analysis; D012503:Saponins / Q000032:analysis*
D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D