Immunobiological activities of a chemically synthesized lipid A of Porphyromonas gingivalis.
A synthetic lipid A of Porphyromonas gingivalis strain 381 (compound PG-381), which is similar to its natural lipid A, demonstrated no or very low endotoxic activities as compared to Escherichia coli-type synthetic lipid A (compound 506). On the other hand, compound PG-381 had stronger hemagglutinating activities on rabbit erythrocytes than compound 506. Compound PG-381 also induced mitogenic responses in spleen cells from lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice, as well as LPS-responsive C3H/HeN mice. The addition of polymyxin B resulted in the inhibition of mitogenic activities, however, compound 506 did not show these capacities. Additionally, compound PG-381 showed a lower level of activity in inducing cytokine production in peritoneal macrophages and gingival fibroblasts from C3H/HeN mice, but not C3H/HeJ mice, in comparison to compound 506. Thus, this study demonstrates that the chemical synthesis of lipid A, mimicking the natural lipid A portion of LPS from P. gingivalis, confirms its low endotoxic potency and immunobiological activity.
Ogawa
T
T
Department of Microbiology, Asahi University, School of Dentistry, Gifu, Japan. tomo527@dent.asahi-u.ac.jp
Asai
Y
Y
Yamamoto
H
H
Taiji
Y
Y
Jinno
T
T
Kodama
T
T
Niwata
S
S
Shimauchi
H
H
Ochiai
K
K
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004731:Endotoxins / Q000633:toxicity; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D005881:Gingiva / Q000166:cytology / Q000276:immunology; D006386:Hemagglutination Tests; D008033:Limulus Test; D008050:Lipid A / Q000138:chemical synthesis / Q000276:immunology* / Q000633:toxicity*; D008213:Lymphocyte Activation; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D016966:Porphyromonas gingivalis / Q000737:chemistry* / Q000276:immunology*; D011817:Rabbits
D016207:Cytokines; D004731:Endotoxins; D008050:Lipid A