title: Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex.
abstract: The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H(t(His)):gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD(t) were determined. As antigens, purified soluble recombinant forms of the gH(t(His)):gL complex produced by insect cells and of gD(t) produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH(t(His)): gL-specific IgG. Using peripheral blood (PB) T cells, gH(t(His)):gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH(t(His)):gL contained high levels of interferon-gamma and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.
authors.affiliation: Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
authors.forename: Douwe F
authors.initials: DF
authors.lastname: Westra
authors.affiliation: Rotterdam Eye Hospital2 and Institute of Virology3, Erasmus University Rotterdam, Rotterdam, The Netherlands.
authors.forename: Georges M G M
authors.initials: GMGM
authors.lastname: Verjans
authors.affiliation: Rotterdam Eye Hospital2 and Institute of Virology3, Erasmus University Rotterdam, Rotterdam, The Netherlands.
authors.forename: Albert D M E
authors.initials: ADME
authors.lastname: Osterhaus
authors.affiliation: Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
authors.forename: Adriaan
authors.initials: A
authors.lastname: van Kooij
authors.affiliation: Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
authors.forename: Gjalt W
authors.initials: GW
authors.lastname: Welling
authors.affiliation: Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
authors.forename: Albert Jan
authors.initials: AJ
authors.lastname: Scheffer
authors.affiliation: Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
authors.forename: T Hauw
authors.initials: TH
authors.lastname: The
authors.affiliation: Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
authors.forename: Sytske
authors.initials: S
authors.lastname: Welling-Wester
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D015847:Interleukin-4; D007371:Interferon-gamma