Development of a recombinant nucleoprotein-based enzyme-linked immunosorbent assay for quantification of antibodies against porcine reproductive and respiratory syndrome virus.
A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.
Witte
S B
SB
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506, USA.
Chard-Bergstrom
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C
Loughin
T A
TA
Kapil
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S
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D009698:Nucleoproteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D009698:Nucleoproteins; D014764:Viral Proteins