title: A novel 'two-component' protein containing histidine kinase and response regulator domains required for sporulation in Aspergillus nidulans.
abstract: We have characterised a-novel Aspergillus nidulans gene encoding a 'two-component' signalling protein (tcsA). tcsA encodes both a histidine kinase domain and a response regulator domain similar to those found in bacterial, lower eukaryotic and plant members of the two-component family of proteins, while two PAS domains in the amino-terminal region of the predicted tcsA product may monitor the signal which regulates a tcsA histidine kinase-response regulator phosphorelay. While tcsA is nonessential for vegetative growth, cells lacking the gene are unable to produce conidia on standard Aspergillus growth media. However, tcsA is not absolutely required for production since this defect is suppressed by growth on 1 M sorbitol.
authors.affiliation: Department of Biochemistry, University of Dundee, UK.
authors.forename: M
authors.initials: M
authors.lastname: Virginia
authors.forename: C L
authors.initials: CL
authors.lastname: Appleyard
authors.forename: W L
authors.initials: WL
authors.lastname: McPheat
authors.forename: M J
authors.initials: MJ
authors.lastname: Stark
mesh_terms: D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D000071677:Histidine Kinase; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology; D017434:Protein Structure, Tertiary; D013172:Spores, Fungal
chemical_list: D005656:Fungal Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase; C415590:TcsA protein, Aspergillus nidulans