Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages.
Pluripotent hematopoietic stem cells have been studied extensively, but the events that occur during their differentiation remain largely uncharted. To develop a system that allows the differentiation of cultured multipotent progenitors by time-lapse fluorescence microscopy, myelomonocytic cells were labeled with green fluorescent protein (GFP) in vivo. This was achieved by knocking the enhanced GFP (EGFP) gene into the murine lysozyme M (lys) locus and using a targeting vector, which contains a neomycin resistant (neo) gene flanked by LoxP sites and "splinked" ends, to increase the frequency of homologous recombination. Analysis of the blood and bone marrow of the lys-EGFP mice revealed that most myelomonocytic cells, especially mature neutrophil granulocytes, were fluorescence-positive, while cells from other lineages were not. Removal of the neo gene through breeding of the mice with the Cre-deleter strain led to an increased fluorescence intensity. Mice with an inactivation of both copies of the lys gene developed normally and were fertile. (Blood. 2000;96:719-726)
Faust
N
N
Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Varas
F
F
Kelly
L M
LM
Heck
S
S
Graf
T
T
D000818:Animals; D002469:Cell Separation; D005434:Flow Cytometry; D005453:Fluorescence; D015870:Gene Expression; D018390:Gene Targeting; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005838:Genotype; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics*; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009113:Muramidase / Q000235:genetics*
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D009113:Muramidase