Synthesis of aminoglycoside-modified oligonucleotides.
[structure: see text] To study the structural requirements of aminoglycoside binding to nucleic acids, compound 1-an analogue of the naturally occurring nucleoside J-was synthesized. When incorporated into oligodeoxynucleotides, 1 leads to thermal stabilization of the resulting duplexes. The increase in pairing affinity is stronger with complementary RNA than with DNA.
Tona
R
R
Department of Chemistry and Biochemistry, University of Bern, Switzerland.
Bertolini
R
R
Hunziker
J
J
D000617:Aminoglycosides / Q000138:chemical synthesis / Q000737:chemistry*; D020029:Base Pairing; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry
D000617:Aminoglycosides; D009841:Oligonucleotides; D012313:RNA; D004247:DNA