title: Use of phosphonic acid as a generic hapten in the production of broad specificity anti-organophosphate pesticide antibody.
abstract: Phosphonic acid (trans-4-phosphono-2-butenic acid; TPB) was used as a generic hapten in order to generate broad specificity antibodies against a group of organophosphorus pesticides. The polyclonal antiserum showed, in an indirect enzyme-linked immunosorbent assay (ELISA) format, preferential binding toward pesticides containing unsaturated diethyl-phosphate functionalities rather than the equivalent thiophosphate or dimethyl structures. The level of detection in the ELISA using a heterologous system was investigated and showed a 20-fold improvement when a conjugate for which the antibody had lower affinity was immobilized on the plate. Biosensor assays using parathion as a standard indicated that the antibody had a relatively high dissociation rate, and reproducible cycles of regeneration were achieved. The potential for using TPB as a generic hapten is discussed.
authors.affiliation: Institute of Food Research, Norwich Research Park, Colney, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Alcocer
authors.forename: P P
authors.initials: PP
authors.lastname: Dillon
authors.forename: B M
authors.initials: BM
authors.lastname: Manning
authors.forename: C
authors.initials: C
authors.lastname: Doyen
authors.forename: H A
authors.initials: HA
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Daly
authors.forename: R
authors.initials: R
authors.lastname: O'Kennedy
authors.forename: M R
authors.initials: MR
authors.lastname: Morgan
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006241:Haptens; D007306:Insecticides / Q000032:analysis* / Q000276:immunology*; D008297:Male; D063065:Organophosphonates / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D006241:Haptens; D007306:Insecticides; D063065:Organophosphonates