[Phenylephrine synchromizes rhythm of protein synthesis in hepatocyte culture].
Published data indicate that 1-3 microM adrenomimetic phenylephrine increases the concentration of calcium ions in the cytoplasm of cultured hepatocytes. We studied low-density cultures exhibiting no protein synthesis rhythm in the fresh medium and demonstrated that a 2 min action of 2 microM phenylephrine induces protein synthesis rhythm, i.e., synchronizes the synthesis oscillations in hepatocytes. A similar effect was observed for a selective inhibitor of the reticulum calcium pump, di-tert-butyl-benzohydroquinone, that increases the cytoplasm concentration of calcium ions by a receptor-independent mechanism. A calcium antagonist imipramine obviated the synchronization effect of phenylephrine. We propose that short-term changes in the cytoplasm concentration of calcium ions covering the whole cell population are among intracellular mechanisms of protein synthesis synchronization in hepatocytes in vitro.
Brodskiĭ
V Ia
VIa
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Nechaeva
N V
NV
Avdonin
P V
PV
Zvezdina
N D
ND
Novikova
T E
TE
Gvazava
I G
IG
Fateeva
V I
VI
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010507:Periodicity; D010656:Phenylephrine / Q000494:pharmacology*; D014176:Protein Biosynthesis; D051381:Rats
D000316:Adrenergic alpha-Agonists; D004791:Enzyme Inhibitors; D010656:Phenylephrine; D000252:Calcium-Transporting ATPases; D002118:Calcium