Epitope mapping of the influenza A virus RNA polymerase PA using monoclonal antibodies.
To obtain reagents to functionally map the PA protein, we produced monoclonal antibodies specific to this protein. Twenty-two monoclonal antibodies reacting with PA protein in ELISA were divided into 10 groups on the basis of competitive binding patterns to this protein. Of these, seventeen monoclonal antibodies bound to PA polypeptide spanning amino acids 101-400 and three bound to that of amino acids 518-600, while the other two did not react with any PA polypeptides tested with the exception of full-length PA. Among these monoclonal antibodies, only five reacted with PA in A/PR/8/34 virus-infected cells in indirect immunofluorescence assay. Thus, we obtained monoclonal antibodies that recognize at least 10 distinct regions of the PA molecule. These monoclonal antibodies should be useful in dissecting functions of the PA protein.
Hatta
M
M
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Asano
Y
Y
Masunaga
K
K
Ito
T
T
Okazaki
K
K
Toyoda
T
T
Kawaoka
Y
Y
Ishihama
A
A
Kida
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002460:Cell Line; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping / Q000379:methods*; D006801:Humans; D009980:Influenza A virus / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D051379:Mice; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D012324:RNA-Dependent RNA Polymerase