Development of conjunctival goblet cells and their neuroreceptor subtype expression.
OBJECTIVE
To investigate expression of muscarinic, cholinergic, and adrenergic receptors on developing conjunctival goblet cells.


METHODS
Eyes were removed from rats 9 to 60 days old, fixed, and used for microscopy. For glycoconjugate expression, sections were stained with Alcian blue/periodic acid-Schiffs reagent (AB/PAS) and with the lectins Ulex europeus agglutinin I (UEA-I) and Helix pomatia agglutinin (HPA). Goblet cell bodies were identified using anti-cytokeratin 7 (CK7). Nerve fibers were localized using anti-protein gene product 9.5. Location of muscarinic and adrenergic receptors was investigated using anti-muscarinic and beta-adrenergic receptors.


RESULTS
At days 9 and 13, single apical cells in conjunctival epithelium stained with AB/PAS, UEA-I, and CK7. At days 17 and 60, increasing numbers of goblet cells were identified by AB/PAS, UEA-I, HPA, and CK7. Nerve fibers were localized around stratified squamous cells and at the epithelial base at days 9 and 13, and around goblet cells and at the epithelial base at days 17 and 60. At days 9 and 13, M2- and M3-muscarinic and beta2-adrenergic receptors were found in stratified squamous cells, but M1-muscarinic and beta1-adrenergic receptors were not detected. At days 17 and 60, M2- and M3-muscarinic receptors were found in goblet cells, whereas M1-muscarinic receptors were in stratified squamous cells. Beta1- and beta2-adrenergic receptors were found on both cell types. Beta3-adrenergic receptors were not detected.


CONCLUSIONS
In conjunctiva, nerves, M2- and M3-muscarinic, and beta1- and beta2-adrenergic receptors are present on developing goblet cells and could regulate secretion as eyelids open.
Ríos
J D
JD
Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA. rios@vision.eri.harvard.edu
Forde
K
K
Diebold
Y
Y
Lightman
J
J
Zieske
J D
JD
Dartt
D A
DA
D000423:Alcian Blue; D000818:Animals; D003228:Conjunctiva / Q000166:cytology / Q000254:growth & development* / Q000294:innervation; D019084:Fluorescent Antibody Technique, Indirect; D006001:Glycoconjugates / Q000378:metabolism; D020397:Goblet Cells / Q000166:cytology / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009882:Ophthalmic Nerve / Q000378:metabolism; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis* / Q000145:classification; D011976:Receptors, Muscarinic / Q000096:biosynthesis* / Q000145:classification; D013869:Thiolester Hydrolases / Q000378:metabolism; D043222:Ubiquitin Thiolesterase
D006001:Glycoconjugates; D037102:Lectins; D009419:Nerve Tissue Proteins; D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic; D007633:Keratins; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase; D000423:Alcian Blue