HPLC-electrospray mass spectrometry for the analysis of temoporfin-poly(ethylene glycol) conjugates.
The photodynamic therapeutic agent temoporfin, 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC) conjugated with poly(ethylene glycol) 2000 (PEG), has been analysed by high performance liquid chromatography (HPLC), linked to electrospray ionisation mass spectrometry (ESI-MS). Sufficient separation of m-THPC-PEG 2000 oligomers was achieved, enabling determination of molecular mass. The use of ESI-MS alone could not achieve this, because of too great a complexity in the mass spectrum, resulting from the presence of four PEG 2000 side chains with a wide molecular mass distribution. The technique is applicable to similar PEG conjugated compounds.
Lord
G A
GA
MRC Toxicology Unit, University of Leicester, UK.
Cai
H
H
Luo
J L
JL
Lim
C K
CK
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008652:Mesoporphyrins / Q000032:analysis* / Q000737:chemistry; D017319:Photosensitizing Agents / Q000032:analysis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry
D008652:Mesoporphyrins; D017319:Photosensitizing Agents; C411426:temoporfin-polyethylene glycol conjugate; D011092:Polyethylene Glycols