title: A novel murine Staufen isoform modulates the RNA content of Staufen complexes.
abstract: Mouse Staufen (mStau) is a double-stranded RNA-binding protein associated with polysomes and the rough endoplasmic reticulum (RER). We describe a novel endogenous isoform of mStau (termed mStau(i)) which has an insertion of six amino acids within dsRBD3, the major double-stranded RNA (dsRNA)-binding domain. With a structural change of the RNA-binding domain, this conserved and widely distributed isoform showed strongly impaired dsRNA-binding ability. In transfected cells, mStau(i) exhibited the same tubulovesicular distribution (RER) as mStau when weakly expressed; however, when overexpressed, mStau(i) was found in large cytoplasmic granules. Markers of the RER colocalized with mStau(i)-containing granules, showing that overexpressed mStau(i) could still be associated with the RER. Cotransfection of mStau(i) with mStau relocalized overexpressed mStau(i) to the reticular RER, suggesting that they can form a complex on the RER and that a balance between these isoforms is important to achieve proper localization. Coimmunoprecipitation demonstrated that the two mStau isoforms are components of the same complex in vivo. Analysis of the immunoprecipitates showed that mStau is a component of an RNA-protein complex and that the association with mStau(i) drastically reduces the RNA content of the complex. We propose that this new isoform, by forming a multiple-isoform complex, regulates the amount of RNA in mStau complexes in mammalian cells.
authors.affiliation: Departments of Biochemistry, University of Montreal, Montreal, Quebec, Canada H3C 3J7.
authors.forename: T
authors.initials: T
authors.lastname: Duchaîne
authors.forename: H J
authors.initials: HJ
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: S V
authors.initials: SV
authors.lastname: Steinberg
authors.forename: I R
authors.initials: IR
authors.lastname: Nabi
authors.forename: L
authors.initials: L
authors.lastname: DesGroseillers
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D013347:Subcellular Fractions
chemical_list: D020033:Protein Isoforms; D016601:RNA-Binding Proteins; C492187:Stau1 protein, mouse; C117884:staufen protein, mammalian; D012313:RNA