Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa.
The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polkappa), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polkappa bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polkappa in the mutagenic bypass of certain types of DNA lesions.
Ohashi
E
E
Institute for Virus Research, Kyoto University, Japan.
Ogi
T
T
Kusumoto
R
R
Iwai
S
S
Masutani
C
C
Hanaoka
F
F
Ohmori
H
H
D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004249:DNA Damage / Q000235:genetics*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D006801:Humans
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C094399:DinB protein, E coli; D029968:Escherichia coli Proteins