Determination of moxifloxacin (BAY 12-8039) in plasma and lung tissue by high-performance liquid chromatography with ultraviolet detection using a fully automated extraction method with a new polymeric cartridge.
The aim of this study was to develop a high-performance liquid chromatographic (HPLC) assay for the determination of moxifloxacin in human plasma and lung tissue. The assay was based on HPLC with a Supelcosil ABZ+ column and ultraviolet detection set at a wavelength of 296 nm. The extraction procedure was characterized by a fully automated liquid-solid extraction using an OASIS column for the solid phase. The assay has been found to be linear and validated over the concentration range 3.2 to 0.025 microg/ml for moxifloxacin in plasma and from 16 to 0.25 microg/g for moxifloxacin in lung tissue. In future, the assay will support the pharmacokinetic study of the penetration of moxifloxacin in human lung tissue.
Lemoine
T
T
Clinical Pharmacokinetic Department, Victor Segalen University, Bordeaux, France.
Breilh
D
D
Ducint
D
D
Dubrez
J
J
Jougon
J
J
Velly
J F
JF
Saux
M C
MC
D042462:4-Quinolones; D000890:Anti-Infective Agents / Q000097:blood / Q000493:pharmacokinetics*; D001372:Aza Compounds; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D024841:Fluoroquinolones; D006801:Humans; D008168:Lung / Q000378:metabolism*; D000077266:Moxifloxacin; D011804:Quinolines; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D042462:4-Quinolones; D000890:Anti-Infective Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D000077266:Moxifloxacin