Identification of differentially regulated genes of Plasmodium by suppression subtractive hybridization.
Plasmodium, the causative agent of malaria, has many morphologically and functionally distinct developmental stages. In the mosquito host alone, there are five transitions during the development of a gametocyte into a sporozoite. Determining which genes are expressed at the different developmental stages is vital to our understanding of the parasite. There are a growing number of techniques designed to study gene expression, including microarray. Here, Johannes Dessens, Gabrielle Margos, Maria del Carmen Rodriguez and Robert Sinden describe a novel method: suppression subtractive hybridization (SSH) and its successful application in obtaining mosquito midgut stage-specific genes of Plasmodium.
Dessens
J T
JT
Imperial College of Science, Technology and Medicine, Department of Biology, Sir Alexander Fleming Building, Imperial College Road, London, UK SW7 2AZ. j.dessens@ic.ac.uk
Margos
G
G
Rodriguez
M C
MC
Sinden
R E
RE
D000818:Animals; D009033:Culicidae / Q000469:parasitology; D018507:Gene Expression Regulation, Developmental; D008288:Malaria / Q000469:parasitology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010961:Plasmodium / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism