Biosynthesis of interleukin-6, an autocrine growth factor for melanoma, is regulated by melanoma-derived histamine.
Interleukin-6 is an autocrine growth factor in advanced stage melanoma and biosynthesis of IL-6 is increased by histamine in various cell lines. In our study we analysed the direct relation of histamine and IL-6 synthesis in human melanoma cell lines. All melanoma cells contained IL-6 mRNA, but only metastatic melanoma cells secreted the IL-6 protein. The H1 histamine receptor antagonist triprolidine decreased gene expression and biosynthesis of IL-6, while other histamine receptor antagonists had no effect. These data indicate that endogenous histamine has a definite role in the regulation of local IL-6, suggesting that histamine and IL-6 could be part of autocrine growth regulation of the tumor.
Molnár
E L
EL
Department of Genetics, Cell- and Immunbiology, Semmelweis University, Budapest, Hungary.
Hegyesi
H
H
Tóth
S
S
Darvas
Z
Z
László
V
V
Szalai
C
C
Falus
A
A
D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism* / Q000627:therapeutic use; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008545:Melanoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D006133:Growth Substances; D015850:Interleukin-6; D012333:RNA, Messenger; D006632:Histamine; D005956:Glucose-6-Phosphate Isomerase