Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
We report here the first demonstration of dengue virus infection and vasoactive cytokine response of a cell of the mast cell/basophil lineage. Infection of KU812 cells was dependent on dengue-specific antibody and gave rise to infectious virions. This antibody-enhanced dengue virus infection triggered a four- to fivefold increase in the release of interleukin-1beta (IL-1beta) and a modest increase for IL-6 but not for an alternate cytokine, granulocyte-macrophage colony-stimulating factor. The results suggest a potential role for mast cells/basophils in the pathogenesis of dengue virus-induced disease.
King
C A
CA
Departments of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada.
Marshall
J S
JS
Alshurafa
H
H
Anderson
R
R
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001491:Basophils / Q000276:immunology / Q000821:virology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008407:Mast Cells / Q000276:immunology / Q000821:virology*; D014709:Vero Cells; D014771:Virion / Q000502:physiology
D000914:Antibodies, Viral; D007375:Interleukin-1; D015850:Interleukin-6