title: Phenotypic alterations of recruited eosinophils in peritoneal fluid eosinophilia.
abstract: OBJECTIVE
  To characterize eosinophils and soluble factors in effluent from continuous ambulatory peritoneal dialysis (CAPD) patients and connect these findings to related conditions with eosinophilic accumulation.
  
  
  METHODS
  Three newly started CAPD patients, two with peritoneal fluid eosinophilia (PFE) and one with bacteria-induced peritonitis. One patient with PFE was followed up for 10 visits during a 7-month period.
  
  
  METHODS
  Leukocytes were analyzed in dialysate and peripheral blood from the patients, by flow cytometry, and soluble mediators by ELISA or CAP technique.
  
  
  RESULTS
  We found an increased number of neutrophils in the effluent from the patient with bacteria-induced peritonitis; accumulation of eosinophils in combination with negative cultures was noted in the patients with PFE. Increased levels of interleukin (IL)-5 and eosinophil cationic protein, but equal levels of eotaxin, were found in effluent from the PFE patients compared to the patient with neutrophilia. Peritoneal fluid eosinophils were activated by means of EG2, CD11b, CD9, and CD69 expression. Compared to blood eosinophils, the cytokine receptors for IL-5 and granulocyte-macrophage colony-stimulating factor, but not IL-3, were down regulated.
  
  
  CONCLUSIONS
  The finding of activated eosinophils in combination with IL-5 and eotaxin in PFE indicates existing similarities between PFE and conditions found during recruitment of eosinophils in allergic inflammatory responses.
authors.affiliation: Division of Clinical Immunology, Department of Laboratory Medicine, Karolinska Hospital, Stockholm, Sweden. cecilia.hellman@mb.ks.se
authors.forename: C
authors.initials: C
authors.lastname: Hellman
authors.forename: J
authors.initials: J
authors.lastname: Lundahl
authors.forename: B
authors.initials: B
authors.lastname: Hylander
authors.forename: G
authors.initials: G
authors.lastname: Halldén
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001202:Ascitic Fluid / Q000166:cytology; D001798:Blood Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D047091:Eosinophil Granule Proteins; D004802:Eosinophilia / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis*; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects*; D010532:Peritoneal Diseases / Q000175:diagnosis* / Q000209:etiology; D010641:Phenotype; D011336:Probability; D012016:Reference Values; D012260:Ribonucleases; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
chemical_list: D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D016209:Interleukin-8; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D012260:Ribonucleases