Photosensitizing properties of palladium-tetraphenylporphycene on cultured tumour cells.
In this study we describe photokilling properties and effects on the mitotic index (MI) of cultured HeLa cells induced by palladium(II)-tetraphenylporphycene (PdTPP(0)). The drug was synthesized by refluxing tetraphenylporphycene (TPP(0)) and PdCl2 in dimethylformamide, followed by evaporation and purification by chromatography. Cells were treated with different concentrations of PdTPPo incorporated into dipalmitoylphosphatidylcholine liposomes, and red light irradiation (lambda > 600 nm) was performed at 21 mW/cm2. No dark toxicity was found when the drug was applied under our experimental conditions. Using lethal (LD(100)) treatments, cells showed the immediate occurrence of bubbles on the plasma membrane, whereas homogeneous nuclear condensation and loss of cytoplasm appeared 3-24 h later. An increased MI was found 6-8 h after sublethal LD(25) and LD(40) treatments as well as a high proportion of abnormal metaphases with altered spindle microtubules. Chromatin condensation and fragmentation were observed 8 h after LD(75) treatments. These results show that in comparison with TPP(0), the new sensitizer PdTPPo has more efficient photokilling properties which could be very valuable for the photodynamic therapy of cancer.
Cañete
M
M
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Canto Blanco, Spain.
Ortiz
A
A
Juarranz
A
A
Villanueva
A
A
Nonell
S
S
Borrell
J I
JI
Teixidó
J
J
Stockert
J C
JC
D015060:1,2-Dipalmitoylphosphatidylcholine; D002453:Cell Cycle / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003624:Darkness; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008027:Light; D008081:Liposomes; D008665:Metalloporphyrins / Q000138:chemical synthesis / Q000633:toxicity*; D017319:Photosensitizing Agents / Q000633:toxicity*; D014407:Tumor Cells, Cultured
D008081:Liposomes; D008665:Metalloporphyrins; D017319:Photosensitizing Agents; C411522:palladium(II)-2,7,12,17-tetraphenylporphycene; D015060:1,2-Dipalmitoylphosphatidylcholine