Biochemical-genetic characterization and distribution of OXA-22, a chromosomal and inducible class D beta-lactamase from Ralstonia (Pseudomonas) pickettii.
From genomic DNA of Ralstonia pickettii isolate PIC-1, a beta-lactamase gene was cloned that encodes the oxacillinase OXA-22. It differs from known oxacillinases, being most closely related to OXA-9 (38% amino acid identity). The hydrolytic spectrum of OXA-22 is limited mostly to benzylpenicillin, cloxacillin, and restricted-spectrum cephalosporins. OXA-22-like genes were identified as single chromosomal copies in five other R. pickettii clinical isolates. The expression of OXA-22-like beta-lactamases was inducible in R. pickettii.
Nordmann
P
P
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France. nordmann.patrice@bct.ap-hop-paris.fr
Poirel
L
L
Kubina
M
M
Casetta
A
A
Naas
T
T
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004790:Enzyme Induction; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; C413942:beta-lactamase OXA-22; D001618:beta-Lactamases