Comparison of antioxidant enzyme activities between Solanum tuberosum L. Cultivars Danshaku and Kitaakari during low-temperature storage.
We compared the antioxidant enzyme activities between Solanum tuberosum L. cultivars Kitaakari and "Danshaku" during storage at 1 degrees C and 20 degrees C. The Kitaakari and Danshaku plants contained approximately 330 microM and 120 microM ascorbic acid (AsA) immediately after the harvest, respectively. At 1 degrees C, the activity of ascorbate peroxidase (APx) in the Kitaakari plants showed the tendency to increase, while in the Danshaku its activity increased temporally by 9 weeks and thereafter returned to basal levels. Superoxide dismutase (SOD) activity increased after 12 weeks in the case of the Kitaakari at 1 degrees C. Catalase did not show any difference in both cultivars at each temperature. The contents of AsA, which was one of the substrates of APx, decreased more rapidly at 1 degrees C than at 20 degrees C in both cultivars. Particularly in the case of the Danshaku, AsA contents were already less than 30 microM at 9 weeks, which confirmed that APx was inactivated.
Kawakami
S
S
Graduate School of Science and Technology, Kobe University, Japan.
Mizuno
M
M
Tsuchida
H
H
D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000032:analysis / Q000378:metabolism; D002374:Catalase / Q000378:metabolism*; D005519:Food Preservation; D005615:Freezing; D010544:Peroxidases / Q000378:metabolism*; D011198:Solanum tuberosum / Q000737:chemistry / Q000201:enzymology*; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism*
D010544:Peroxidases; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid