title: Differential immediate-early gene responses to shear stress and intraluminal pressure in intact human conduit vessels.
abstract: We have previously shown distinct effects of shear stress and pressure on transcription of several potent vascular mediators. In the present study, we tested the hypothesis that c-jun and c-fos are regulated differentially by shear and pressure. Intact human umbilical veins were perfused with various combinations of shear and pressure during 1.5, 3 and 6 h. Protein and gene expressions were assessed by immunofluorescence and real-time reverse transcription PCR, respectively. Shear stress and pressure exert differential temporal effects on c-jun and c-fos gene and protein expression, and these immediate-early gene responses appear to be cell-type specific for endothelial and smooth muscle cells.
authors.affiliation: Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Ostra, Göteborg University, SE 416 85, Göteborg, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Gan
authors.forename: R
authors.initials: R
authors.lastname: Doroudi
authors.forename: U
authors.initials: U
authors.lastname: Hägg
authors.forename: A
authors.initials: A
authors.lastname: Johansson
authors.forename: L
authors.initials: L
authors.lastname: Selin-Sjögren
authors.forename: S
authors.initials: S
authors.lastname: Jern
mesh_terms: D004730:Endothelium, Vascular / Q000378:metabolism* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early / Q000235:genetics*; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D006439:Hemodynamics / Q000502:physiology; D006801:Humans; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D009130:Muscle, Smooth / Q000378:metabolism; D009928:Organ Specificity; D010477:Perfusion; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013314:Stress, Mechanical; D013997:Time Factors; D014471:Umbilical Veins; D015854:Up-Regulation
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger