Antimicrobial susceptibility of staphylococci isolated from otitis externa in dogs.
Samples were obtained from 65 unmedicated adult dogs, processed for isolation of Staphylococcus species and tested for susceptibility to penicillin G, gentamicin, oxacillin, tetracycline, trimethoprim-sulphamethoxazole, streptomycin, ampicillin and rifampin. Forty-four isolates were obtained, which represents 67.7% of samples. Coagulase-negative species were most commonly found, and the most frequently isolated staphylococcus species were Staph. epidermidis and Staph. aureus. Other species, such as Staph. simulans, Staph. haemolyticus, Staph. saprophyticus and Staph. intermedius were also isolated. Resistance to antibiotics was frequently observed, with 90.9% of the isolates showing resistance to at least one drug. The most active antimicrobial agents against staphylococci isolated from otitis externa of dogs were rifampin and oxacillin. Multidrug resistance was a common finding, and one strain of Staph. haemolyticus species, was resistant to all tested antimicrobial agents. Resistance to three or more different drugs was a common finding, observed in 16 strains (36.4%) of both coagulase-positive and coagulase-negative staphylococci. This study highlights the emergence of cases of otitis externa determined by coagulase-negative staphylococcus strains and once more emphasizes the need for bacterial culture with species identification and susceptibility testing of swab specimens from the ear canal in order to choose appropriate antimicrobial agents.
Lilenbaum
W
W
Laboratorio de Bacteriologia, Instituto Biomédico, Universidade Federal Fluminense, Niteroi, RJ, Brazil. mipwalt@vm.uff.br
Veras
M
M
Blum
E
E
Souza
G N
GN
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003030:Coagulase / Q000032:analysis; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D005260:Female; D008297:Male; D008826:Microbial Sensitivity Tests; D010032:Otitis Externa / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000187:drug effects* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D003030:Coagulase