title: A role of carboxy-terminal region of Toxoplasma gondii-heat shock protein 70 in enhancement of T. gondii infection in mice.
abstract: We investigated the role of recombinant Toxoplasma gondii heat shock protein (rT.g.HSP) 70-full length, rT.g.HSP70-NH2-terminal region, or rT.g.HSP70-carboxy-terminal region in prophylactic immunity in C57BL/6 mice perorally infected with Fukaya cysts of T. gondii. At 3, 4, 5, and 6 weeks after infection, the number of T. gondii in the brain tissue of each mouse was measured by quantitative competitive-polymerase chain reaction (QC-PCR) targeting the surface antigen (SAG) 1 gene. Immunization with rT.g.HSP70-full length or r.T.g.HSP70-carboxy-terminal region increased the number of T. gondii in the brain tissue after T. gondii infection, whereas immunization with rT.g.HSP70-NH2-terminal region did not. These results suggest that T.g.HSP70-carboxy-terminal region as well as T.g.HSP70-full length may induce deleterious effects on the protective immunity of mice infected with a cyst-forming T. gondii strain, Fukaya.
authors.affiliation: Department of Parasitology, Chiba University School of Medicine, Japan.
authors.forename: H S
authors.initials: HS
authors.lastname: Mun
authors.forename: K
authors.initials: K
authors.lastname: Norose
authors.forename: F
authors.initials: F
authors.lastname: Aosai
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Yano
mesh_terms: D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014122:Toxoplasma / Q000502:physiology*; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000469:parasitology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D011994:Recombinant Proteins