Dialyzable leukocyte extract suppresses the activity of essential transcription factors for HIV-1 gene expression in unstimulated MT-4 cells.
The human immunodeficiency virus type 1 (HIV-1) contains regulatory regions in its long terminal repeat (LTR) implicated in the control of viral gene expression. We previously demonstrated that Dialyzable Leukocyte Extract (DLE), a preparation derived from immune leukocytes, is able to inhibit HIV-1 replication in MT-4 cell cultures. Here, we examined the effect of DLE on the activation of NF-kappaB and Sp1 transcription factors. NF-kappaB activity was completely suppressed after seven days of treatment with 2.5 U/mL of DLE, with a parallel large reduction in the amounts of Sp1 complexes. These findings correlate with the maximum inhibitory effect on HIV-1 replication described in a previous report. IkappaBalpha and NF-kappaB p65(RelA) gene expression are not regulated by DLE in MT-4 cells. Although up to day, the precise molecular mechanism of DLE biological activity in HIV-1 infection remains unclear, this report presents data that indicate a potential downregulatory effect of DLE on HIV-1 gene expression.
Ojeda
M O
MO
Cell Biology Division, Center for Biological Research, Havana, Cuba.
Fernández-Ortega
C
C
Rosaínz
M J
MJ
D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology*; D014165:Transfer Factor / Q000494:pharmacology*
D017931:DNA Primers; D016328:NF-kappa B; D014157:Transcription Factors; D014165:Transfer Factor