Assay for Laccase activity by microcalorimetry: laccase was extracted from china lacquer of Rhus vernicifera.
The reactions between Laccase (extracted from China lacquer of Rhus vernicifera) and various substrates (3,4-Dihydroxybenzaldehyde, Guaiacol, Pyrogallol, Gallic acid) have been studied using LKB-2107 batch microcalorimetry system. Based on calorimetry, a new method has been proposed. Laccase activity and the Michaelis constant K(m) have been determined simultaneously by this method. The method is simple, sample-saving, and valid for a wider range of substrate concentrations. Furthermore, it can be extended for assaying other enzymes catalyzing reactions using this method.
Wang
T
T
Department of Chemistry, Central China Normal University, 430079, Wuhan, PR China. tzwang@mnr.whcnc.ac.cn
Li
W
W
Wan
H W
HW
Zhou
P J
PJ
Qu
S S
SS
D002151:Calorimetry / Q000379:methods*; D007700:Kinetics; D042845:Laccase; D010088:Oxidoreductases / Q000378:metabolism*; D010947:Plants, Toxic; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D012251:Toxicodendron / Q000201:enzymology*
D010088:Oxidoreductases; D042845:Laccase