title: Viability and functional activity of cryopreserved mononuclear cells.
abstract: Factors that influence viability and function of cryopreserved peripheral blood mononuclear cells (PBMC) were identified on 54 samples from 27 AIDS Clinical Trial Units. PBMC viability ranged from 1 to 96% with a median of 70%, was higher in laboratories with experienced staff, and was not significantly associated with CD4 cell number. Function of cryopreserved PBMC, measured by lymphocyte proliferation, was associated with viability. Preparations with viability greater than or equal to 70% had consistent proliferative responses and were suitable for functional analyses.
authors.affiliation: University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. adriana.weinberg@uchsc.edu
authors.forename: A
authors.initials: A
authors.lastname: Weinberg
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: A
authors.initials: A
authors.lastname: Erice
authors.forename: B
authors.initials: B
authors.lastname: Polsky
authors.forename: M S
authors.initials: MS
authors.lastname: Hirsch
authors.forename: S
authors.initials: S
authors.lastname: Owens
authors.forename: K
authors.initials: K
authors.lastname: Lamb
mesh_terms: D002455:Cell Division; D002470:Cell Survival; D015925:Cryopreservation; D006801:Humans; D007963:Leukocytes, Mononuclear