title: Nature of the residue of [(14)C]Cloransulam-methyl in lactating goats.
abstract: Two lactating goats were given a daily oral dose of either [UL-aniline-(14)C; AN] or [triazolopyrimidine-7,9-(14)C; TP]cloransulam-methyl for 5 consecutive days. Each animal received a dietary equivalent of approximately 10 mg/kg of test material, approximately 2225 times the realistic maximum dietary exposure for a dairy animal. Milk, urine, and feces samples were collected in the morning and afternoon for each animal. Each goat was sacrificed within 23 h of receiving the last dose, and the liver, kidneys, samples of blood, fat, muscle, and gastrointestinal tract contents, and urine from the bladder were collected. All of these samples were analyzed for (14)C content. Cloransulam-methyl (CM) was rapidly excreted by the animals, with 99.9% of the recovered radioactivity appearing in the urine and feces. Radiochemical analysis showed very low residues, with the highest being in the kidneys at 0.122 and 0. 128 mg equiv of CM/kg (AN and TP labeled compounds, respectively). Radioactive residues were extracted and fractionated from kidney, liver, and milk. Analysis showed approximately 0.066 mg/kg CM in the kidney but <0.003 mg/kg in the liver. Only one metabolite, cloransulam, was identified (in liver, 9.5% of total radioactive residue; 0.005 mg/kg). All other metabolites were present at lower levels. Sulfonanilide bridge cleavage was not a significant degradation route for cloransulam-methyl in ruminants. These data indicated a very low bioaccumulation potential for cloransulam-methyl and its metabolites in ruminants. For a ruminant exposed to anticipated levels of cloransulam-methyl in its diet, parent and metabolites, in total, would not be expected to exceed 50 ng/kg in the kidney and liver.
authors.affiliation: Dow AgroSciences, Indianapolis, IN 46268, USA. plewer@dowagro.com
authors.forename: P
authors.initials: P
authors.lastname: Lewer
authors.forename: K L
authors.initials: KL
authors.lastname: Finney-Brink
authors.forename: D O
authors.initials: DO
authors.lastname: Duebelbeis
mesh_terms: D000818:Animals; D001565:Benzoates / Q000493:pharmacokinetics*; D002250:Carbon Radioisotopes; D003612:Dairying; D004350:Drug Residues / Q000493:pharmacokinetics*; D005260:Female; D006041:Goats; D006540:Herbicides / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism; D007774:Lactation; D008099:Liver / Q000378:metabolism; D008657:Metabolic Clearance Rate; D008892:Milk / Q000737:chemistry; D011865:Radioisotope Dilution Technique; D013449:Sulfonamides / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D001565:Benzoates; D002250:Carbon Radioisotopes; D006540:Herbicides; D013449:Sulfonamides; C412472:cloransulam-methyl