title: IL-6 promoter is modulated by the 24 kDa FGF-2 isoform fused to the hormone binding domain of the oestrogen receptor.
abstract: Fusion proteins consisting of the 24 kDa nuclear form of basic fibroblast growth factor (FGF-2), associated with the hormone binding domain of oestrogen receptor (HBD), convey oestrogen inducibility to FGF-2. When stable HBD-FGF-2 HeLa cell lines were transiently transfected with an interleukin 6 (IL-6) construct, the IL-6 promoter activity was downregulated by the addition of oestradiol. Moreover, in these cell lines, the function of the FGF-2 nuclear localisation sequence was abolished by its fusion to HBD, while addition of oestradiol restored the location of the chimera to the nucleus.
authors.affiliation: INSERM U397, Institut C. Regaud, Toulouse, France.
authors.forename: I
authors.initials: I
authors.lastname: Delrieu
authors.forename: P
authors.initials: P
authors.lastname: Chinestra
authors.forename: F
authors.initials: F
authors.lastname: Delassus
authors.forename: F
authors.initials: F
authors.lastname: Bayard
authors.forename: H
authors.initials: H
authors.lastname: Prats
authors.forename: J C
authors.initials: JC
authors.lastname: Faye
mesh_terms: D001665:Binding Sites; D015153:Blotting, Western; D015536:Down-Regulation; D004958:Estradiol / Q000378:metabolism / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004967:Estrogens; D015850:Interleukin-6; D020033:Protein Isoforms; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D016222:Fibroblast Growth Factor 2; D004958:Estradiol