title: Rapid simultaneous determination of glucagon and insulin by capillary electrophoresis immunoassays.
abstract: A rapid capillary electrophoresis (CE) with laser-induced fluorescence (LIF) competitive immunoassay has been developed for the determination of glucagon in biological mixtures. In the assay, fluorescein-conjugated glucagon is mixed with the sample followed by addition of anti-glucagon. Free and antibody-bound, tagged glucagon could be separated in 3 s using CE to obtain quantitative determination of glucagon with a concentration detection limit of 760 pM. The assay was combined with a previously developed competitive immunoassay for insulin to produce a simultaneous immunoassay for both peptides. The method was used to determine glucagon content of islets of Langerhans.
authors.affiliation: Department of Chemistry, University of Florida, Gainesville 32611-7200, USA.
authors.forename: I
authors.initials: I
authors.lastname: German
authors.forename: R T
authors.initials: RT
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D017022:Flow Injection Analysis; D005934:Glucagon / Q000032:analysis*; D007118:Immunoassay; D007328:Insulin / Q000032:analysis*; D051379:Mice
chemical_list: D007328:Insulin; D005934:Glucagon