Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography.
A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).
Gimeno
E
E
Dpt. Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Spain.
Castellote
A I
AI
Lamuela-Raventós
R M
RM
de la Torre
M C
MC
López-Sabater
M C
MC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E / Q000032:analysis*
D010938:Plant Oils; D014810:Vitamin E