CDw108 expression during T-cell development.
We recently reported a gene encoding the human CDw108, a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that is preferentially expressed on activated T lymphocytes and erythrocytes. The present study investigated the expression of CDw108 on various tissues and cells, particularly on T cells during development. The murine CDw108 cDNA was cloned initially, and it was highly homologous to the human CDw108 (88.0% or 89.3% similarity at the nucleotide or amino acid level, respectively) or identical to the murine semaphorin K1/Sema7A. The CDw108 mRNA was demonstrated in a few tissues including thymus and brain with the highest expression coming on day 7 in whole embryo followed by relatively consistent expression during development. Cell-surface expression of the CDw108 during T-cell development was further examined by flow cytometry in the human umbilical cord blood and thymus. It was preferentially expressed on a CD34+ stem cell population of umbilical cord blood, and CD3dull CD34+/- CD117 (c-kit)+ CD4bright CDbright cells in the thymus that are involved in the stage of positive selection. These results suggest the contribution of CDw108 in T-cell development, especially in the stage of positive selection in the thymus.
Mine
T
T
Kurume University Research Center for Innovative Cancer Therapy, Department of Surgery, Kurume University School of Medicine, Japan.
Harada
K
K
Matsumoto
T
T
Yamana
H
H
Shirouzu
K
K
Itoh
K
K
Yamada
A
A
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology*; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D018507:Gene Expression Regulation, Developmental / Q000276:immunology*; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008074:Lipoproteins / Q000235:genetics* / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry / Q000276:immunology; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D039961:Semaphorins; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000737:chemistry / Q000276:immunology; D013601:T-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D013737:Testis / Q000737:chemistry / Q000276:immunology; D013950:Thymus Gland / Q000737:chemistry / Q000166:cytology / Q000276:immunology
D015703:Antigens, CD; D018076:DNA, Complementary; D058851:GPI-Linked Proteins; D006023:Glycoproteins; D008074:Lipoproteins; D012333:RNA, Messenger; C118753:SEMA7A protein, human; C485571:Sema7a protein, mouse; D039961:Semaphorins