A role of iron ions in the SOS DNA repair response induced by nitric oxide in Escherichia coli.
An induction of the SOS DNA repair response by physiological nitric oxide donors (dinitrosyl iron complexes (DNIC) with thiols and S-nitrosothiols (RSNO)) was studied in E. coli cells. DNIC with thiols were the most effective SOS-inducers. Being more toxic, RSNO mediated a similar response at 10-100 microM, but they were inactive at concentrations above 0.5 mM. Pretreatment of the cells with chelating agents, o-phenanthroline and picolinic acid, prevented induction of the SOS response by all NO-donors used and led to a decrease in the DNIC-type EPR signal that appeared after incubation of the cells with DNIC or S-nitrosoglutathione (GSNO). Analysis of these effects revealed a dual role of iron ions in reactivity and toxicity of the NO-donating agents. On one hand, they could stabilize GSNO in the form of less toxic DNIC, and, on the other hand, they took part in the formation of the SOS-inducing signal by NO-donating agents.
Stupakova
M V
MV
Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 117334, Russia.
Lobysheva
I I
II
Mikoyan
V D
VD
Vanin
A F
AF
Vasilieva
S V
SV
D004260:DNA Repair / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D007501:Iron / Q000502:physiology*; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D013014:SOS Response, Genetics
D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D007501:Iron