Analysis of unique variable region of a plant root inducing plasmid, pRi1724, by the construction of its physical map and library.
Ri plasmids in Agrobacterium rhizogenes specifically induce the hairy root syndrome on various dicotyledonous plants. Its T-DNA transfer system as well as those of Ti plasmids have successfully provided the fundamental technique to introduce exogenous genes into plants. To study the Ri genome structure, we constructed a complete BamHI physical map and a lambda library of pRi1724 of A. rhizogenes strain 1724. By using these, we carried out the complete sequence of the 74-kb region between the right border of T-DNA and tra operon, which is the highly variable region (VAR) among Ri and Ti plasmids. As a result, we found three kinds of putative ABC-type transport operons, histidine utilization operon, glycerol utilization operon and two chemoreceptor genes. In addition, a virulence-related gene, tzs was located independently of the vir region.
Moriguchi
K
K
Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan.
Maeda
Y
Y
Satou
M
M
Kataoka
M
M
Tanaka
N
N
Yoshida
K
K
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D015723:Gene Library; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D020161:Physical Chromosome Mapping; D010957:Plasmids / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018528:ATP-Binding Cassette Transporters