title: Possible involvement of cholinergic and adrenergic mechanisms in changing contractility of guinea pig ileum by Ipomoea carnea.
abstract: The study aimed to elucidate the mechanism (s) of action of Ipomoea carnea leaf juice (ILJ) in changing contractility of guinea pig ileum. ILJ produced dose-dependent (10-10000 microg/ml) triphasic responses. The initial contractile phase was blocked by atropine (1 microg/ml) but had additive effect with acetylcholine (2 ng/ml) or carbachol (2 ng/ml). Neostigmine (30 ng/ml) or lignocaine (50 microg/ml) failed to alter the response. In cold-induced denervated preparations, this phase was augmented. The relaxatory phase of ILJ was not modified by phenoxybenzamine (35 microg/ml) but was reduced by propranolol (1 microg/ml) and abolished by lignocaine (50 microg/ml). The final contractile phase of ILJ was not affected by atropine (1 microg/ml). These results suggest that the triphasic response of ILJ is possibly mediated through cholinergic, adrenergic and non-cholinergic mechanisms, respectively.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Science and A.H., I.G.A.U., Anjora, -491001 (MP), Durg, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Hore
authors.forename: R
authors.initials: R
authors.lastname: Ottalwar
authors.forename: K M
authors.initials: KM
authors.lastname: Koley
authors.forename: A K
authors.initials: AK
authors.lastname: Pathak
mesh_terms: D018663:Adrenergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects; D018678:Cholinergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D018663:Adrenergic Agents; D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D018678:Cholinergic Agents; D010936:Plant Extracts