title: Effects of cholecystokinin on acid formation in glands and cells isolated from rabbit and rat gastric mucosa.
abstract: Isolated gastric glands and isolated cells prepared from rabbit and rat were studied to analyse the influence of cholecystokinin octapeptide (CCK 8) on histamine stimulated parietal cell acid formation as assessed by [14C]aminopyrine sequestered in acid tissue compartments. In rabbit gastric glands, CCK 8 evoked 32+/-6% (P<0. 01) inhibition of histamine stimulated acid formation, whereas in glands prepared from rat no inhibition was recorded. Instead, CCK 8 seemed to induce a variable increase of the histamine stimulation in rat gastric glands as the aminopyrine accumulation was increased by 110+/-46% (P<0.1). Further studies on cell preparations derived from rabbit gastric mucosa revealed dual properties of CCK 8, eliciting either inhibition or stimulation of the parietal cell depending on the presence of endocrine cells. The results show that paracrine communication may be effective in glandular preparations, but seems to vary depending on species.
authors.affiliation: Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, Sweden. per.bengtsson@eu.pnu.com
authors.forename: P
authors.initials: P
authors.lastname: Bengtsson
authors.forename: L
authors.initials: L
authors.lastname: Azerkan
authors.forename: G
authors.initials: G
authors.lastname: Lundqvist
authors.forename: G
authors.initials: G
authors.lastname: Nilsson
authors.forename: S
authors.initials: S
authors.lastname: Mårdh
mesh_terms: D000632:Aminopyrine / Q000493:pharmacokinetics; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics; D002452:Cell Count; D002478:Cells, Cultured; D002766:Cholecystokinin / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019858:Enteroendocrine Cells / Q000166:cytology / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D010295:Parietal Cells, Gastric / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012844:Sincalide / Q000378:metabolism / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000632:Aminopyrine; D013004:Somatostatin; D006632:Histamine; D002766:Cholecystokinin; D012844:Sincalide