Characterization of the DNA replication module of bacteriophage A2 and use of its origin of replication as a defense against infection during milk fermentation by Lactobacillus casei.
Adjacent to the lysis/lysogeny cassette of the A2 phage genome lies a stretch of over 8 kb, which contains a series of genes probably involved in DNA replication. Fifteen open reading frames (orfs) were identified, 13 of which are encoded on the main coding strand and only two on the complementary strand. Database searches and comparative analyses allowed the identification of an open reading frame (orf455) that shows similarity with DNA helicases and contains a variant zinc-finger motif known from the phage T7 helicase/primase. Orf770 showed similarity to putative plasmid and phage DNA primases. Downstream of orf770 is a noncoding 258-bp region rich in direct and inverted repeats, which specifically binds to proteins whose synthesis is induced during phage infection. When present in a plasmid, this region can direct a partial bacteriophage resistance phenotype due to interference with phage DNA replication, both under laboratory conditions and during milk fermentation. It is deduced that this stretch contains the origin of replication of phage A2.
Moscoso
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Area de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, c/Julián Clavería s/n, Oviedo, E-33006, Spain.
Suárez
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JE
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D016208:Databases, Factual; D005285:Fermentation; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000821:virology*; D008892:Milk / Q000378:metabolism* / Q000382:microbiology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins