Selectivity release of the type II decoy IL-1 receptor.
The type II IL-1 receptor (IL-1RII) is a non-signalling molecule which acts as a decoy target for IL-1. Various signals (e.g. chemoattractants and phorbol ester) induce metalloprotease-mediated rapid shedding of the IL-1RII. The present study was designed to compare the susceptibility to shedding of the decoy IL-1RII vs the signalling IL-1RI. HEK 293 cells and COS cells were transfected with cDNAs encoding the human IL-1RI, IL-1RII or both. Slow spontaneous release and rapid phorbol ester-induced shedding were only observed for the decoy IL-1RII. Similarly, OVCAR-3 cells, which express substantial amounts of both IL-1RI and IL-1RII, only released the IL-1RII. These results indicate that the IL-RII, but not the IL-1RI, is susceptible to proteolytic shedding, a finding consistent with the decoy function of this molecule.
Orlando
S
S
Department of Immunology and Cell Biology, Istituto di Ricerche Farmacologiche 'Mario Negri', Milano, Italy.
Polentarutti
N
N
Mantovani
A
A
D000818:Animals; D019556:COS Cells; D002460:Cell Line; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D013755:Tetradecanoylphorbol Acetate