Cloning, structural organization, and chromosomal assignment of the porcine c-fos proto-oncogene, FOS.
The complete porcine c-fos proto-oncogene (FOS) with flanking regions was cloned and sequenced. FOS consists of four exons at amino acids 1-47, 48-131, 132-167, and 168-380 and includes all the typical motifs of the fos proto-oncogene. The promoter contains consensus sequences for CRE, SRE, CaRE, and the E-Box, as well as an AP-1 site. Homologies between human and swine were between 89.7% and 96.3% in the exons. Based on somatic cell hybrid panel screening and known homologies between swine chromosome 7 and human chromosome 14, the porcine c-fos gene was assigned to chromosome 7q23.
Reiner
G
G
Department of Animal Breeding and Biotechnology, University of Hohenheim, Stuttgart, Germany. g-reiner@uni-hohenheim.de
Heinricy
L
L
Brenig
B
B
Geldermann
H
H
Dzapo
V
V
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics*; D016762:Genes, fos / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D020218:Response Elements / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*; D016385:TATA Box / Q000235:genetics