Cytotoxic effects of 5-fluorocytosine on melanoma cells transduced with cytosine deaminase gene.
To investigate the cytotoxic effects of 5-fluorocytosine on melanoma cells genetically modified with cytosine deaminase gene, the gene was transduced into the tumor cells with the retroviral method. The cytotoxicity effects of 5-fluorocytosine on the tumor cells were measured with the MTT assay and clonogenic assay. It was found that the prodrug 5-fluorocytosine had significant cytotoxic effects on melanoma cells transduced with cytosine deaminase in vitro. The IC50 value of 5-fluorocytosine on transgenic and nontransgenic melanoma cells was 572 microg/mL(-1) and 3870 microg/mL(-1), respectively. Our experiment demonstrated the potential value of the cytosine deaminase gene/5-fluorocytosine system in the treatment of melanoma.
Lü
H
H
Department of Clinical Pharmacology, Affiliated North Taiping Hospital, Beijing, China.
Wu
D
D
Wan
Y
Y
Gu
B
B
Wang
J
J
D016475:3T3 Cells; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D043525:Cytosine Deaminase; D004354:Drug Screening Assays, Antitumor; D005437:Flucytosine / Q000494:pharmacology*; D008546:Melanoma, Experimental / Q000473:pathology; D051379:Mice; D009700:Nucleoside Deaminases / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
D000963:Antimetabolites; D005437:Flucytosine; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase