Universal bases for hybridization, replication and chain termination.
Several unnatural, predominantly hydrophobic nucleobases that pack efficiently in duplex DNA without hydrogen bonding functionalities are reported to circumvent the hydrogen bonding-based specificity, both during oligonucleotide hybridization and enzymatic DNA synthesis. The reported nucleoside analogs are efficient 'universal bases' for hybridization, template directed DNA synthesis and chain termination. Moreover, several of the universal bases function in their biological role, hybridization or replication, with an efficiency not significantly reduced relative to their natural counterparts.
Berger
M
M
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Wu
Y
Y
Ogawa
A K
AK
McMinn
D L
DL
Schultz
P G
PG
Romesberg
F E
FE
D020029:Base Pairing; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D003845:Deoxycytosine Nucleotides / Q000378:metabolism; D004355:Drug Stability; D006358:Hot Temperature; D006860:Hydrogen Bonding; D007546:Isoquinolines / Q000737:chemistry; D008745:Methylation; D009693:Nucleic Acid Hybridization; D009705:Nucleosides / Q000737:chemistry / Q000378:metabolism; D009841:Oligonucleotides / Q000378:metabolism; D013379:Substrate Specificity; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism
D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D007546:Isoquinolines; D009705:Nucleosides; D009841:Oligonucleotides; D013942:Thymine Nucleotides; C024107:2'-deoxycytidine 5'-triphosphate; C008179:isocarbostyril; D004247:DNA; D004259:DNA-Directed DNA Polymerase; C026600:2'-deoxyadenosine triphosphate; C024157:thymidine 5'-triphosphate