title: The Drosophila SHC adaptor protein is required for signaling by a subset of receptor tyrosine kinases.
abstract: Receptor tyrosine kinases (RTKs) transduce signals via cytoplasmic adaptor proteins to downstream signaling components. We have identified loss-of-function mutations in dshc, the Drosophila homolog of the mammalian adaptor protein SHC. A point mutation in the phosphotyrosine binding (PTB) domain completely abolishes DSHC function and provides in vivo evidence for the function of PTB domains. Unlike other adaptor proteins, DSHC is involved in signaling by only a subset of RTKs: dshc mutants show defects in Torso and DER but not Sevenless signaling, which is confirmed by epistasis experiments. We show by double-mutant analysis that the adaptors DOS, DRK, and DSHC act in parallel to transduce the Torso signal. Our results suggest that DSHC confers specificity to receptor signaling.
authors.affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abteilung Genetik, Tübingen, Germany. stefan.luschnig@tuebingen.mpg.de
authors.forename: S
authors.initials: S
authors.lastname: Luschnig
authors.forename: J
authors.initials: J
authors.lastname: Krauss
authors.forename: K
authors.initials: K
authors.lastname: Bohmann
authors.forename: I
authors.initials: I
authors.lastname: Desjeux
authors.forename: C
authors.initials: C
authors.lastname: Nüsslein-Volhard
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins; D004843:Epistasis, Genetic; D066246:ErbB Receptors; D005123:Eye / Q000196:embryology; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010053:Ovary / Q000196:embryology; D011494:Protein Kinases; D011506:Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D018010:Receptors, Invertebrate Peptide; D055765:Shc Signaling Adaptor Proteins; D015398:Signal Transduction; D020664:Son of Sevenless Proteins / Q000378:metabolism; D014921:Wings, Animal / Q000196:embryology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; C100319:Dos protein, Drosophila; D029721:Drosophila Proteins; D005136:Eye Proteins; D019476:Insect Proteins; D011506:Proteins; D018010:Receptors, Invertebrate Peptide; D055765:Shc Signaling Adaptor Proteins; D020664:Son of Sevenless Proteins; C080530:drk protein, Drosophila; D011494:Protein Kinases; C066532:Egfr protein, Drosophila; D066246:ErbB Receptors; D020794:Receptor Protein-Tyrosine Kinases; C080148:tor protein, Drosophila