title: MEX-5 and MEX-6 function to establish soma/germline asymmetry in early C. elegans embryos.
abstract: An asymmetrical network of cortically localized PAR proteins forms shortly after fertilization of the C. elegans egg. This network is required for subsequent asymmetries in the expression patterns of several proteins that are encoded by nonlocalized, maternally expressed mRNAs. We provide evidence that two nearly identical genes, mex-5 and mex-6, link PAR asymmetry to those subsequent protein asymmetries. MEX-5 is a novel, cytoplasmic protein that is localized through PAR activities to the anterior pole of the 1-cell stage embryo. MEX-5 localization is reciprocal to that of a group of posterior-localized proteins called germline proteins. Ectopic expression of MEX-5 is sufficient to inhibit the expression of germline proteins, suggesting that MEX-5 functions to inhibit anterior expression of the germline proteins.
authors.affiliation: Zoology Department, University of Washington, Seattle 98109, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Schubert
authors.forename: R
authors.initials: R
authors.lastname: Lin
authors.forename: C J
authors.initials: CJ
authors.lastname: de Vries
authors.forename: R H
authors.initials: RH
authors.lastname: Plasterk
authors.forename: J R
authors.initials: JR
authors.lastname: Priess
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning; D017173:Caenorhabditis elegans / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins; D016764:Cell Polarity; D005854:Germ Cells; D015801:Helminth Proteins / Q000235:genetics / Q000302:isolation & purification*; D008954:Models, Biological; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; C410587:MEX-5 protein, C elegans; C410589:MEX-6 protein, C elegans