Simultaneous determination of losartan and hydrochlorothiazide in tablets by high-performance liquid chromatography.
A method for the simultaneous determination of losartan potassium and hydrochlorothiazide in tablets is described. The procedure, based on the use of reversed-phase high-performance liquid chromatography, is linear in the concentration range 3.0-7.0 microg ml(-1) for losartan and 0.5-2.0 microg ml(-1) for hydrochlorothiazide, is simple and rapid and allows accurate and precise results. The limit of detection was 0.08 microg ml(-1) for losartan and 0.05 microg ml(-1) for hydrochlorothiazide.
Carlucci
G
G
Dipartimeto di Chimica, Ingegneria Chimica e Materiali, Università dell'Aquila, Coppito (L'Aquila), Italy. carlucci@aquila.infn.it
Palumbo
G
G
Mazzeo
P
P
Quaglia
M G
MG
D000959:Antihypertensive Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004232:Diuretics; D006852:Hydrochlorothiazide / Q000032:analysis*; D019808:Losartan / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D049993:Sodium Chloride Symporter Inhibitors / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D000959:Antihypertensive Agents; D004232:Diuretics; D049993:Sodium Chloride Symporter Inhibitors; D006852:Hydrochlorothiazide; D019808:Losartan