The wound healing properties of Channa striatus-cetrimide cream-- tensile strength measurement.
Channa striatus, a fresh water snakehead fish, is reported to enhance dermal wound healing. Biochemical components such as amino acids and fatty acids are important for the synthesis of collagen fibers during wound healing. Arachidonic acid, a precursor of prostaglandin plays a vital role in healing the wounds. Haruan (C. striatus) contains all the essential amino acids for wound healing particularly glycine as well as high contents of arachidonic acid and polyunsaturated fatty acids that can promote prostaglandin synthesis. In the present work we have studied the wound healing effect of C. striatus in Sprague-Dawley rats. Cream formulations having different haruan fish extract concentrations as the active ingredient were prepared and stabilized, and they were applied to the wounds. The healing of wounds was characterized by an increase in the tensile strength of the skin, determined on the 7th post-operative day in each case. Haruan treatment of wounds promotes remodeling of collagen, by the synthesis of inter- and intra-molecular protein crosslinking and thus produces a marked increase (P<0.05) in tensile strength as compared to the cetrimide treated group. On the basis of our experiment we conclude that C. striatus helps in wound healing as indicated by the increase in tensile strength. We hypothesise that this effect may be due to its high content of arachidonic acid, glycine and polyunsaturated fatty acids. The mechanism of wound healing will be investigated in future studies.
Baie
S H
SH
Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia. saringat@usm.my
Sheikh
K A
KA
D000818:Animals; D002498:Centrifugation; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology*; D004355:Drug Stability; D005399:Fishes / Q000502:physiology*; D008297:Male; D009824:Ointments; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology; D012867:Skin / Q000473:pathology*; D013268:Stimulation, Chemical; D013696:Temperature; D013718:Tensile Strength; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000473:pathology*
D002593:Cetrimonium Compounds; D009824:Ointments; D000077286:Cetrimonium