Distribution of carbonic anhydrase isozyme VI in the developing bovine parotid gland.
The distribution of bovine carbonic anhydrase isozyme VI (CA-VI), purified from bovine saliva, was studied immunohistochemically using antiserum against bovine CA-VI in bovine parotid glands during fetal and postnatal development. A weak expression of CA-VI in undifferentiated epithelial cells and ductal cells was observed in a 4- to 5-month-old fetus with a 26-cm crown-rump length. The reaction in both acinar and ductal cells subsequently persisted during late gestation and birth. Although anti-CA-VI reactivity was still seen in both regions immediately following birth, the reactivity had almost completely disappeared from most duct segments by 1 month following birth. Changes in the localization and time-dependent expression of the isozyme in parotid glands may reflect changes in the biological function of structurally closely related isozymes.
Asari
M
M
Department of Anatomy and Physiology, Azabu University, School of Veterinary Medicine, Fuchinobe, Sagamihara, Japan. asari@azabu-u.ac.jp
Miura
K
K
Ichihara
N
N
Nishita
T
T
Amasaki
H
H
D000818:Animals; D000831:Animals, Newborn; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002417:Cattle; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism; D010306:Parotid Gland / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development
D007527:Isoenzymes; D002256:Carbonic Anhydrases