Mitotic replication initiation proteins are not required for pre-meiotic S phase.
Initiation of mitotic DNA replication in eukaryotes requires conserved factors, including Cdc18/CDC6 and minichromosome maintenance (MCM) proteins. We show here that these proteins are not essential for meiotic DNA replication or subsequent meiotic divisions in fission yeast. In addition, vegetative replication checkpoint genes are not required for the arrest of meiotic divisions in response to pre-meiotic S-phase delays. Genes essential for other aspects of vegetative DNA replication, however, including polymerases and DNA ligase, are also required for pre-meiotic DNA synthesis. Our results indicate that the process of replication initiation and checkpoint control may be fundamentally different in mitotic and meiotic cells.
Forsburg
S L
SL
Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA. forsburg@salk.edu
Hodson
J A
JA
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D000071877:Checkpoint Kinase 1; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis; D004268:DNA-Binding Proteins; D004171:Diploidy; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008540:Meiosis / Q000502:physiology*; D064148:Minichromosome Maintenance Complex Component 4; D008938:Mitosis / Q000502:physiology*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D011995:Recombination, Genetic; D016196:S Phase; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D011503:Transglutaminases
D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C082463:RAD4 protein, S pombe; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C082462:cdc18 protein, S pombe; D011503:Transglutaminases; D011494:Protein Kinases; C087019:ran1 protein, S pombe; D000071877:Checkpoint Kinase 1; D017346:Protein Serine-Threonine Kinases; C092802:MCM4 protein, S cerevisiae; D064148:Minichromosome Maintenance Complex Component 4