Retinoic acid upregulates beta(1)-integrin in vascular smooth muscle cells and alters adhesion to fibronectin.
Retinoic acid has an established physiological role in differentiation, development, and cellular growth. This study investigated the action of all-trans retinoic acid (ATRA) on vascular integrins, cell-surface receptors that control growth and remodeling of blood vessels. The beta(1)-integrin subunit mRNA and protein was induced after treatment with ATRA in two different rat vascular smooth muscle cell lines. To relate this result to the in vivo state, the aortas from adult rats fed with therapeutic doses of ATRA were examined for beta(1)-integrin protein. A significant upregulation of the integrin subunit was observed in vivo. To assess if this increase contributed to physiological changes in cellular function, cells treated with ATRA were tested for alterations in adhesion to extracellular matrix proteins. The cells exposed to the retinoid were seen to adhere more strongly to fibronectin, via the beta(1)-integrin. These results showed that modulation of vascular integrins by ATRA in adult rats contributes to functional changes that can cause remodeling of blood vessels.
Medhora
M M
MM
Department of Physiology, Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA. medhoram@mcw.edu
D000818:Animals; D001011:Aorta; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D005353:Fibronectins / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D019012:Integrin beta1 / Q000235:genetics* / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D011651:Pulmonary Artery; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*
D005353:Fibronectins; D019012:Integrin beta1; D012333:RNA, Messenger; D014212:Tretinoin