Inhibition of fungal cell wall synthesizing enzymes by trans-cinnamaldehyde.
This study examined the inhibitory effects of trans-cinnamaldehyde (CA), an aromatic aldehyde derived from Cinnamomi Cortex, on Saccharomyces cerevisiae cell wall synthesizing enzymes in vitro. This compound was found to be a noncompetitive inhibitor of beta-(1,3)-glucan synthase and a mixed inhibitor of chitin synthase 1 with 50% inhibitory concentrations (IC50) of 0.84 and 1.44 mM, respectively. Chitin synthases 2 and 3 were less sensitive than chitin synthase 1 to CA. CA can be useful as a model compound of cell wall inhibitors for the development of effective antifungal agents.
Bang
K H
KH
Department of Microbiology, College of Natural Sciences, Chungnam National University, Yusong, Taejon, Korea.
Lee
D W
DW
Park
H M
HM
Rhee
Y H
YH
D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D002473:Cell Wall / Q000378:metabolism*; D002687:Chitin Synthase / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors*; D008565:Membrane Proteins; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins; D013237:Stereoisomerism
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins; D000171:Acrolein; D005964:Glucosyltransferases; D002687:Chitin Synthase; C020570:1,3-beta-glucan synthase; C012843:cinnamaldehyde