A protein factor of rat liver mitochondrial matrix involved in flavinylation of dimethylglycine dehydrogenase.
The involvement of rat liver mitochondria in the flavinylation of the mitochondrial matrix flavoenzyme dimethylglycine dehydrogenase (Me2GlyDH) has been investigated. Me2GlyDH was synthesized as an apoenzyme in the rabbit reticulocyte lysate (RL) transcription/translation system and its flavinylation was monitored by virtue of the trypsin resistance of the holoenzyme. The rate of holoenzyme formation in the presence of FAD was stimulated with increasing efficiency by the addition of solubilized mitoplasts, mitochondrial matrix and DEAE-purified matrix fraction. Apo-Me2GlyDH was also converted into holoenzyme when the solubilized mitoplasts were supplemented with FMN and ATP. This observation is consistent with the existence of a mitochondrial FAD synthetase generating the FAD needed for holoenzyme formation from its precursors. Holoenzyme formation in the presence of FAD increased linearly with the concentration of matrix protein in the assay, and depended on the amount of externally added Me2GlyDH with saturation characteristics. These findings suggest the presence of a protein factor in the mitochondrial matrix which stimulates Me2GlyDH flavinylation. This factor was different from both mitochondrial heat shock protein (Hsp)70, as shown by immunodepletion experiments, and mitochondrial Hsp60, as demonstrated by the capability of a DEAE-purified matrix fraction devoid of Hsp60 to accelerate flavinylation of both RL translated and purified Me2GlyDH.
Brizio
C
C
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy.
Otto
A
A
Brandsch
R
R
Passarella
S
S
Barile
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001051:Apoenzymes / Q000737:chemistry; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000378:metabolism; D002846:Chromatography, Affinity; D050893:Dimethylglycine Dehydrogenase; D005486:Flavin Mononucleotide / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005415:Flavins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000737:chemistry* / Q000201:enzymology; D024101:Mitochondrial Proteins; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D010957:Plasmids; D014176:Protein Biosynthesis; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012156:Reticulocytes / Q000201:enzymology; D013997:Time Factors; D014158:Transcription, Genetic
D001051:Apoenzymes; D018834:Chaperonin 60; D005415:Flavins; D018840:HSP70 Heat-Shock Proteins; D024101:Mitochondrial Proteins; D005182:Flavin-Adenine Dinucleotide; D005486:Flavin Mononucleotide; D000255:Adenosine Triphosphate; D010089:Oxidoreductases, N-Demethylating; D050893:Dimethylglycine Dehydrogenase; C494400:Dmgdh protein, rat