title: The Saccharomyces cerevisiae RDN1 locus is sequestered from interchromosomal meiotic ectopic recombination in a SIR2-dependent manner.
abstract: Meiotic ectopic recombination occurs at similar frequencies among many sites in the yeast genome, suggesting that all loci are similarly accessible to homology searching. In contrast, we found that his3 sequences integrated in the RDN1 (rDNA) locus were unusually poor participants in meiotic recombination with his3 sequences at other sites. We show that the low rate of meiotic ectopic recombination resulted from the poor ability of RDN1::his3 to act as a donor sequence. SIR2 partially repressed interchromosomal meiotic ectopic recombination at RDN1, consistent with its role in regulating recombination, gene expression, and retrotransposition within RDN1. We propose that RDN1 is physically sequestered from meiotic homology searching mechanisms.
authors.affiliation: Gene Regulation and Chromosome Biology Laboratory, National Institutes of Health, National Cancer Institute, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA. edwardda@intra.niddk.nih.gov
authors.forename: E S
authors.initials: ES
authors.lastname: Davis
authors.forename: B K
authors.initials: BK
authors.lastname: Shafer
authors.forename: J N
authors.initials: JN
authors.lastname: Strathern
mesh_terms: D000483:Alleles; D004275:DNA, Ribosomal / Q000235:genetics*; D004171:Diploidy; D005787:Gene Frequency; D006238:Haploidy; D006655:Histone Deacetylases / Q000378:metabolism* / Q000494:pharmacology; D006836:Hydro-Lyases / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics*; D018626:Retroelements / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D015534:Trans-Activators / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004275:DNA, Ribosomal; D018626:Retroelements; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D015534:Trans-Activators; C087778:SIR2 protein, S cerevisiae; D056565:Sirtuin 2; D037761:Sirtuins; D006655:Histone Deacetylases; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase