An integrated deletion and physical map encompassing l71Rl, a chromosome 7 locus required for peri-implantation survival in the mouse.
l71Rl, a locus that maps just proximal to the pink-eyed dilution (p) locus in mouse chromosome 7, was initially identified as being required for early post-implantation survival. We define further the null phenotype of l71Rl as peri-implantation lethal, with homozygous mutant embryos degenerating between embryonic day 4.5 (E4.5) and E5. 5. We constructed an integrated deletion/physical map covering a 1. 82-Mb chromosomal segment extending proximally from p. This map defines the minimum critical interval for l71Rl as an 80- to 300-kb region. This sequence-ready deletion/physical map should enable the cloning and characterization of the l71Rl gene(s).
Wu
M
M
Life Sciences Division, University of Tennessee-Oak Ridge, Oak Ridge, Tennessee 37831-8077, USA.
Rinchik
E M
EM
Johnson
D K
DK
D000818:Animals; D002875:Chromosomes / Q000235:genetics*; D020451:Contig Mapping; D010064:Embryo Implantation / Q000235:genetics*; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005260:Female; D005313:Fetal Death / Q000235:genetics; D017353:Gene Deletion; D006720:Homozygote; D008297:Male; D051379:Mice; D009115:Muridae; D009154:Mutation; D020161:Physical Chromosome Mapping; D011247:Pregnancy