Levels and repair of cyclobutane pyrimidine dimers and 6-4 photoproducts in skin of sporadic basal cell carcinoma patients.
The 32P-postlabeling method was applied to measure directly the levels and repair rates of specific cyclobutane pyrimidine dimers and 6-4 photoproducts in 10 basal cell carcinoma patients and 10 controls matched on age, skin type, and gender after exposure to 400 J per m2 of solar simulating radiation on previously unexposed buttock skin. The results showed an identical level of photoproducts at 0 h after solar simulating radiation in the basal cell carcinoma group and the control group. Erythemal response correlated with the repair of cyclobutane pyrimidine dimers within 24 h in both groups, i.e., repair was faster in those with a strong erythemal reaction. The basal cell carcinoma patients showed a somewhat slower repair of photoproducts in skin compared with the controls, but the result was not significant. Photoproducts formed at the TTC sites were repaired faster than those at the TTT sites for both cyclobutane pyrimidine dimers and 6-4 photoproducts in the basal cell carcinoma group and in the controls.
Xu
G
G
Department of Biosciences at Novum, Karolinska Institute, Huddinge, Sweden. Guogang.xu@csb.ki.se
Snellman
E
E
Jansen
C T
CT
Hemminki
K
K
D000368:Aged; D002280:Basal Cell Carcinoma / Q000737:chemistry*; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics / Q000378:metabolism*; D004890:Erythema / Q000503:physiopathology; D006801:Humans; D008875:Middle Aged; D011740:Pyrimidine Dimers / Q000235:genetics / Q000378:metabolism*; D012878:Skin Neoplasms / Q000737:chemistry*
D011740:Pyrimidine Dimers; C098727:pyrimidine(6-4)pyrimidone photolyase; D004255:Deoxyribodipyrimidine Photo-Lyase