title: Expression of neuronal nitric oxide synthase in several cell types of the rat gastric epithelium.
abstract: The aim of this study was to identify which cell types of the rat gastric epithelium express neuronal nitric oxide synthase (nNOS) because the results of the previous studies have been very divergent regarding this point. By the combination of immunohistochemical (IHC) and in situ hybridization (ISH) techniques, we detected expression of nNOS in chief and mucosecretory cells of the gastric epithelium. Moreover, some gastric endocrine cells were immunoreactive for nNOS, although they could not be distinguished in sections treated with ISH techniques. The strongest signal for all antibodies in IHC techniques was obtained when microwave (MW) heating was performed before the IHC procedure. Our results indicate that in the gastric epithelium a variety of cell types are able to produce NO. The NO produced by the different cell types (chief, mucous, and endocrine) may form a complex network of paracrine communication with an important role in gastric physiology.
authors.affiliation: Departments of Cytology and Histology, University of Navarra, Pamplona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Vitoria
authors.forename: C
authors.initials: C
authors.lastname: García-Corchón
authors.forename: J A
authors.initials: JA
authors.lastname: Rodríguez
authors.forename: F
authors.initials: F
authors.lastname: García-Amigot
authors.forename: M A
authors.initials: MA
authors.lastname: Burrell
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology*; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat