Opposite effects of antimicrotubule agents on c-myc oncogene expression depending on the cell lines used.
We investigated the expression of c-myc in HT29-D4, HBL100 and Caco-2 cells treated with microtubule stabilising (paclitaxel) or depolymerising agents (vinblastine, nocodazole). After induction by epidermal growth factor (EGF), c-myc expression decreased in HT29-D4 cells treated with all the antimicrotubule agents. In HBL100 and Caco-2, when microtubules were stabilised with paclitaxel, c-myc expression also decreased. In contrast, its expression increased after treatment with depolymerising agents. In both cell lines, we also observed that depolymerising agents alone induced c-myc expression whilst paclitaxel had no effect. This mRNA induction was confirmed at the protein level. In HT29-D4, no variation of c-myc expression was observed. Then, we showed that the increase of mRNA level was due to activation of gene transcription. These results indicate that modulation of c-myc expression varied depending on the cell lines used and the type of antimicrotubule agents. This work provides a potential link between the microtubular network and c-myc gene expression.
Bourgarel-Rey
V
V
UPRES-A CNRS 6032 Faculté de Pharmacie, Marseille, France.
El Khyari
S
S
Rimet
O
O
Bordas
B
B
Guigal
N
N
Braguer
D
D
Seree
E
E
Barra
Y
Y
Briand
C
C
D000230:Adenocarcinoma / Q000188:drug therapy*; D015153:Blotting, Western; D018938:Caco-2 Cells / Q000378:metabolism; D003110:Colonic Neoplasms / Q000188:drug therapy*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc / Q000235:genetics*; D019073:HT29 Cells / Q000378:metabolism; D006801:Humans; D008870:Microtubules / Q000187:drug effects*; D016133:Polymerase Chain Reaction / Q000379:methods; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger