title: Anaplastic osteosarcoma with abundant eosinophilic cytoplasm and minimal osteoid production.
abstract: A case of osteosarcoma with unusual microscopic features, occurring in the right proximal tibial metaphysis of a 12-year-old boy is reported. Radiographically, the tumor was ill-defined and purely osteolytic. On gross examination, the tumor was soft, fragile, spongy and red to brown in color. Microscopically, the tumor consisted of pleomorphic cells possessing abundant eosinophilic cytoplasm, including cells larger than 100 microm in diameter. The cells were arranged in a sheet, with few extracellular collagen fibers. Multiple sectioning of the specimens revealed a small amount of osteoid production. Immunohistochemical study revealed a positive reaction for vimentin and osteocalcin. Electron microscopic study suggested the fibroblastic or osteoblastic origin of the cells.
authors.affiliation: Department of Orthopaedic Surgery, Kobe University School of Medicine, Kobe, Japan. yamatetu@med.kobe-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Marui
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Kizaki
authors.forename: R
authors.initials: R
authors.lastname: Minami
authors.forename: K
authors.initials: K
authors.lastname: Hanioka
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D014408:Biomarkers, Tumor / Q000032:analysis; D001859:Bone Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000628:therapy; D002648:Child; D002945:Cisplatin / Q000008:administration & dosage; D003593:Cytoplasm / Q000473:pathology*; D004317:Doxorubicin / Q000008:administration & dosage; D004359:Drug Therapy, Combination; D004801:Eosine Yellowish-(YS); D017809:Fatal Outcome; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D008727:Methotrexate / Q000008:administration & dosage; D009363:Neoplasm Proteins / Q000032:analysis; D012516:Osteosarcoma / Q000737:chemistry / Q000473:pathology* / Q000628:therapy; D013194:Staining and Labeling; D013977:Tibia / Q000000981:diagnostic imaging / Q000473:pathology; D014057:Tomography, X-Ray Computed
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D004317:Doxorubicin; D002945:Cisplatin; D004801:Eosine Yellowish-(YS); D008727:Methotrexate