A simple and rapid extraction of high molecular weight chromosomal DNA from Bacillus subtilis protoplasts for cosmid cloning and interspecific transformation.
After conversion of Bacillus subtilis vegetative cells to protoplasts, a simple and rapid method for extracting high-molecular-weight chromosomal DNA was devised with the inclusion of bovine serum albumin and phenol-chloroform treatments. The DNA sample thus prepared was the size of 100-450 kb and could be used for cosmid cloning and interspecific transformation.
Akamatsu
T
T
Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan. akamatsu@bio.kumamoto-it.ac.jp
Taguchi
H
H
Okada
H
H
D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D011523:Protoplasts; D012710:Serum Albumin, Bovine; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D012710:Serum Albumin, Bovine