Pax4 represses pancreatic glucagon gene expression.
The paired box and homeodomain containing transcription factors Pax4 and Pax6 are known to be essential for development of the pancreatic endocrine cells. In this report we demonstrate that stable expression of Pax4 in a rat glucagon-producing cell line inhibits the endogenously expressed glucagon gene completely. Furthermore, Pax4 represses Pax6 independent transcription of the insulin promoter, suggesting that Pax4 can actively repress transcription in addition to acting by competition with the transcriptional activator Pax6.
Petersen
H V
HV
Department of Developmental Biology, Hagedorn Research Institute, Niels Steensensvej 6, Gentofte, DK-2820, Denmark.
Jørgensen
M C
MC
Andersen
F G
FG
Jensen
J
J
F-Nielsen
T
T
Jørgensen
R
R
Madsen
O D
OD
Serup
P
P
D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000502:physiology; D005136:Eye Proteins; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005934:Glucagon / Q000235:genetics* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D007328:Insulin / Q000235:genetics; D007515:Islets of Langerhans / Q000378:metabolism*; D051379:Mice; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D007328:Insulin; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C110218:Pax4 protein, mouse; C491708:Pax4 protein, rat; C000605732:Pax6 protein, mouse; C000605733:Pax6 protein, rat; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D005934:Glucagon