[Studies of the development of Triticum aestivum-Leymus racemosus translocation lines by pollen irradiation].
Pollens of 94G15 and 94G45, two wheat lines added to chromosome Lr.14 and Lr.2 of Leymus racemosus respectively were irradiated via 60Co-gamma rays and then used to hybridize with two common wheat varieties--Yangmai 5 and Mianyang 11. The five plants showing chromosome pairing between T. aestivum and L. racemosus at PMC MI stage were selected from seventeen examined plants of M1 progeny as the result of meiosis configuration analysis of chromosomes treated with Giemsa C-banding and fluorescent in situ hybridization. Among which, two T. aestivum-L. racemosus alien translocation lines--LW8(3)1 and LW11(3)1 were developed on the basis of further identification of M2 RTC chromosomes by C-banding and in situ hybridization. In addition, feasibility and effectiveness of the pollen irradiation in the development of T. aestivum-relatives alien translocations and the availability of the translocation lines of T. aestivum-L. racemosus are also discussed.
Liu
W X
WX
Key Lab. of Crop Cytogenetics, CMA, Nanjing Agricultural University, China.
Chen
P D
PD
Liu
D J
DJ
D002871:Chromosome Banding; D017403:In Situ Hybridization; D011058:Pollen / Q000528:radiation effects; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*