title: Identification of peptides in aggregates formed during hydrolysis of beta-lactoglobulin B with a Glu and Asp specific microbial protease.
abstract: The purpose of the present study was to identify the peptides responsible for aggregate formation during hydrolysis of beta-lactoglobulin by BLP at neutral pH. Hydrolysates taken at various stages of aggregate formation were separated into a precipitate and a soluble phase and each was analyzed by CE and mass spectrometry. The aggregates consisted of six to seven major peptides of which four were tentatively identified. The peptides were positively charged at neutral pH and had a high charge-to-mass ratio at low pH. The fragment f135-158 seemed to be the initiator of aggregation, since it was present at high concentration in the aggregates at all stages, and the concentration of this peptide remained low in the supernatant. F135-158 contains several basic and acid amino acids alternating with hydrophobic amino acids, which is in accordance with formation of noncovalently linked aggregates, as previously shown.
authors.affiliation: Department of Dairy and Food Science, The Royal Veterinary and Agricultural University, Rolighedsvej, Frederiksberg, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Otte
authors.forename: S B
authors.initials: SB
authors.lastname: Lomholt
authors.forename: T
authors.initials: T
authors.lastname: Halkier
authors.forename: K B
authors.initials: KB
authors.lastname: Qvist
mesh_terms: D000595:Amino Acid Sequence; D001224:Aspartic Acid; D001407:Bacillus / Q000201:enzymology; D010450:Endopeptidases / Q000378:metabolism*; D018698:Glutamic Acid; D006868:Hydrolysis; D007782:Lactoglobulins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
chemical_list: D007782:Lactoglobulins; D010446:Peptide Fragments; D001224:Aspartic Acid; D018698:Glutamic Acid; D010450:Endopeptidases