Inhibition of the lethal and myotoxic activities of Crotalus durissus terrificus venom by Tabernaemontana catharinensis: identification of one of the active components.
In Brazilian folk medicine, victims of bites by poisonous animals are usually treated with plant extracts derived from the diverse national flora. The chemical and pharmacological properties of most extracts were yet not investigated. In the rural community of Assis-SP, the root bark of Tabernaemontana catharinensis ("leiteiro", "cow milk") is applied to the site of the snake bite and believed to neutralize the effect of the venom. We report here the ability of the lyophilized aqueous extract (AE) and of a pure compound obtained from the ethanolic extract of T. catharinensis to inhibit the lethal and myotoxic activities of C. d. terrificus (South American rattlesnake) venom. Doses of 10 mg AE/100 g, injected (i.m., rat) 20 s after injecting (i.m.) the venom and that of 2.5 mg AE/100 g, incubated for 1 h at 25 degrees C with the venom before injection (i.m.) were able to neutralize the lethal activity of 2LD50. These data indicate that T. catharinensis could be used as a source of a model molecule able to neutralize the lethality and myotoxicity induced by C. d. terrificus venom. Its ethanolic extract was then fractionated on a silica gel 60 chromatography column affording fractions A to F. Fraction A consisted basically of non-polar compounds, terpenes and sterols. Fraction D showed a pronounced antiophidian activity which was later correlated with the presence of the quaternary alkaloid 12-methoxy-4-methylvoachalotine in this fraction. This alkaloid was isolated and inhibited 100% lethality when injected 20 s after 2 LD50 at 1.7 mg/100 g.
Batina
M de F
Mde F
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto, Brazil.
Cintra
A C
AC
Veronese
E L
EL
Lavrador
M A
MA
Giglio
J R
JR
Pereira
P S
PS
Dias
D A
DA
França
S C
SC
Sampaio
S V
SV
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000931:Antidotes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002227:Carbazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D003402:Creatine Kinase / Q000097:blood; D003435:Crotalid Venoms / Q000037:antagonists & inhibitors* / Q000506:poisoning; D007928:Lethal Dose 50; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011041:Poisoning / Q000188:drug therapy / Q000401:mortality; D051381:Rats; D017208:Rats, Wistar; D014197:Trees / Q000737:chemistry*
C412802:12-methoxy-4-methylvoachalotine; D000470:Alkaloids; D000931:Antidotes; D002227:Carbazoles; D003435:Crotalid Venoms; D010936:Plant Extracts; D003402:Creatine Kinase