title: Identification of differentially regulated genes of Plasmodium by suppression subtractive hybridization.
abstract: Plasmodium, the causative agent of malaria, has many morphologically and functionally distinct developmental stages. In the mosquito host alone, there are five transitions during the development of a gametocyte into a sporozoite. Determining which genes are expressed at the different developmental stages is vital to our understanding of the parasite. There are a growing number of techniques designed to study gene expression, including microarray. Here, Johannes Dessens, Gabrielle Margos, Maria del Carmen Rodriguez and Robert Sinden describe a novel method: suppression subtractive hybridization (SSH) and its successful application in obtaining mosquito midgut stage-specific genes of Plasmodium.
authors.affiliation: Imperial College of Science, Technology and Medicine, Department of Biology, Sir Alexander Fleming Building, Imperial College Road, London, UK SW7 2AZ. j.dessens@ic.ac.uk
authors.forename: J T
authors.initials: JT
authors.lastname: Dessens
authors.forename: G
authors.initials: G
authors.lastname: Margos
authors.forename: M C
authors.initials: MC
authors.lastname: Rodriguez
authors.forename: R E
authors.initials: RE
authors.lastname: Sinden
mesh_terms: D000818:Animals; D009033:Culicidae / Q000469:parasitology; D018507:Gene Expression Regulation, Developmental; D008288:Malaria / Q000469:parasitology; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010961:Plasmodium / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism