Ectopic expression of c-ski disrupts gastrulation and neural patterning in zebrafish.
The c-ski proto-oncogene encodes a transcriptional regulator that has been implicated in the development of different tissues at different times during vertebrate development. We identified two novel paralogues of the c-ski gene family, skiA and skiB in zebrafish (Danio rerio). The skiA protein is maternal and ubiquitous while skiB is zygotic. Overexpression of SkiA or SkiB disrupted gastrulation and resulted in a dorsalized phenotype. In situ analyses suggested that overexpression of Ski leads to a slight expansion of dorsal-axial mesoderm, diminishment or loss of ventral mesoderm and radialization of dorsal neuroectoderm. The dorsalized phenotype could be rescued by the ventral specifying factor, BMP4. These results provide evidence that Ski proteins participate in dorsal-ventral specification of both neuroectoderm and mesoderm.
Kaufman
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Department of Genetics, Cell Biology and Development, University of Minnesota, 1445 Gortner Avenue, St. Paul, MN 55108-1095, USA.
Martínez-Rodriguez
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Hackett
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PB
D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005775:Gastrula; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors