title: Expression of CD44 variants in lung cancer and its relationship to hyaluronan binding.
abstract: We examined the expression of CD44 variant forms and their binding to hyaluronan (HA) in lung cancer cell lines. There was no relationship between the level of expression of CD44 variants and HA binding in different lung cancer cell lines. The expression of CD44v6 and CD44E in some cell lines was not always associated with HA binding. There was no relationship between the tissue pathological type and CD44 expression or HA binding. Deglycosylation by neuraminidase induced CD44-HA binding in human lung cancer cell lines. Our findings suggest that the HA binding ability of CD44, which is negatively regulated by glycosylation, might be a more important factor in tumorigenesis or metastasis than the expression of CD44 variant forms.
authors.affiliation: Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsubara
authors.forename: S
authors.initials: S
authors.lastname: Katoh
authors.forename: H
authors.initials: H
authors.lastname: Taniguchii
authors.forename: M
authors.initials: M
authors.lastname: Oka
authors.forename: J
authors.initials: J
authors.lastname: Kadota
authors.forename: S
authors.initials: S
authors.lastname: Kohno
mesh_terms: D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D008175:Lung Neoplasms; D020033:Protein Isoforms / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D020033:Protein Isoforms; D006820:Hyaluronic Acid