title: Phenotypic characterization of monocyte subpopulations in the pig.
abstract: We have recently described the existence of two subsets of porcine monocytes based on the expression of CD163. In this study we compare the expression of a number of cell surface antigens in CD163+ and CD163- monocyte subsets using three-color flow cytometry. These monocyte subsets show differences with respect to the expression of MHC class II antigens (SLA-DR and DQ) and a variety of adhesion molecules (CD11a, wCD11c, wCD29, CD49d) that are expressed at higher levels on CD163+ monocytes, and of CD14 that is higher expressed by CD 163- cells. These differences on phenotype could reflect differences in the ability of these two subsets to migrate to tissues and may account for the higher allostimulatory capacity of CD163+ cells. In some aspects, the phenotype of CD163+ monocytes resembles that of mature macrophages. In vitro serum-induced maturation of monocytes into macrophages lead to the expression of SWC9 together with an increase in the expression of CD163 and a reduction in that of CD14. These results delineate a maturation pathway where CD14hiCD163-SWC9- monocytes develop into CD14loCD163+SWC9- monocytes and these cells into CD14loCD163+SWC9+ macrophages.
authors.affiliation: Centro de Investigación en Sanidad Animal (INIA), Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Chamorro
authors.forename: C
authors.initials: C
authors.lastname: Revilla
authors.forename: B
authors.initials: B
authors.lastname: Alvarez
authors.forename: L
authors.initials: L
authors.lastname: López-Fuertes
authors.forename: A
authors.initials: A
authors.lastname: Ezquerra
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D016130:Immunophenotyping; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D013552:Swine / Q000276:immunology*
chemical_list: D015703:Antigens, CD