title: Genetic analysis of infectious salmon anaemia virus (ISAV) from Scotland.
abstract: The nucleotide sequences of segments 2 and 8 of infectious salmon anaemia virus (ISAV) isolates from Scotland were determined. These were used to predict amino acid sequences of the products of these segments. The sequences were compared with those previously published for ISAV from Norway and North America. Nucleotide and amino acid variations were found in the Scottish isolate, indicating that it is not identical to those Norwegian or North American strains. Although phylogenetic analysis of the sequences was not possible, it was clear that the Scottish isolate is more closely related to the Norwegian strain than the North American. Sequencing further isolates that are geographically or temporally separated will be important in advancing understanding of the epidemiology of this important disease.
authors.affiliation: Fisheries Research Services (FRS) Marine Laboratory, Aberdeen, Scotland, UK. cunninghamc@marlab.ac.uk
authors.forename: C O
authors.initials: CO
authors.lastname: Cunningham
authors.forename: M
authors.initials: M
authors.lastname: Snow
mesh_terms: D000595:Amino Acid Sequence; D000740:Anemia / Q000662:veterinary* / Q000821:virology; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D016000:Cluster Analysis; D017931:DNA Primers / Q000737:chemistry; D005393:Fish Diseases / Q000821:virology*; D007668:Kidney / Q000166:cytology / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009975:Orthomyxoviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D020317:Salmo salar; D012606:Scotland; D016415:Sequence Alignment / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D014764:Viral Proteins