Role of pHyde novel gene product as an intrinsic factor for apoptotic pathway in prostate cancer.
Induction of apoptotic cell death mechanism can be regulated by internal factor(s), such as by gene product(s) that directly upregulate the apoptosis pathway or indirectly by down-regulating the anti-apoptosis gene. This homeostasis is a normal phenomenon in a biological system disturbed by cancer. It is thus important to find any gene functioning as an upregulator for the apoptosis pathway that may have a potential application in the context of cancer gene therapy. We have cloned a novel rat gene, denoted as pHyde, that fulfilled this objective. Internally, this pHyde gene product renders the stable transfectant of rat prostatic cancer cell lines more susceptible to apoptosis even without any external inducer. By using an external agent, such as 5-fluoro-2'-deoxyuridine (FdUr), apoptotic responses of the stable transfectants are even higher, suggesting that both intrinsic and extrinsic factors work synergistically. The pHyde gene product was termed an intrinsic factor, whereas FdUr was considered an extrinsic factor for the apoptosis in rat prostate cancer model.
Rinaldy
A R
AR
University of Tennessee Urologic Research Laboratories, College of Medicine, University of Tennessee, Memphis 38163, USA. Arinaldy@UTMEM1.UTMEM.EDU
Menon
R P
RP
Lerner
J L
JL
Steiner
M S
MS
D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D004249:DNA Damage; D004260:DNA Repair; D005468:Fluorodeoxyuridylate / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007437:Intrinsic Factor / Q000235:genetics / Q000502:physiology*; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured
D005468:Fluorodeoxyuridylate; D007437:Intrinsic Factor