In vitro reversion of amphotericin B resistance in Leishmania donovani by poloxamer 188.
A micellar formulation of amphotericin B (AmB) solubilized with poloxamer 188 was evaluated against an AmB Leishmania donovani-resistant line. A concave isobologram showed a synergistic effect of this association against promastigotes. This result was confirmed with amastigotes since the 50% effective concentration of the new formulation was 100 times less than that of the control AmB formulation.
Espuelas
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Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Navarra, 31080 Pamplona, Spain.
Legrand
P
P
Loiseau
P M
PM
Bories
C
C
Barratt
G
G
Irache
J M
JM
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001622:Betaine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004352:Drug Resistance, Microbial; D004357:Drug Synergism; D020128:Inhibitory Concentration 50; D007893:Leishmania donovani / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D020442:Poloxamer / Q000494:pharmacology*
D000981:Antiprotozoal Agents; D020442:Poloxamer; C066009:desintegron B; D001622:Betaine; D000666:Amphotericin B