[The expression of arrhythmic related genes on Xenopus oocytes for evaluation of class III antiarrhythmic drugs from ocean active material].
By techniques of gene clone, microinjection and patch-clamp, human arrhythmic related gene Kv1.5 and Kv4.2 are translated mRNA, and injected into Xenopus Oocytes, respectively. A pure and single K+ current of ultrarapid delayed rectifier K+ current (Ikur) or transient outward K+ current (Ito) is respectively expressed on Xenopus Oocytes. This is a modern pharmacological model for evaluation class III antiarrhythmic drugs, overcomes many defects such as lacking human fresh cardiac muscle cells as material and co-expression of many currents on cell membrane. A new compound A1998 from Ocean material is evaluated in this model for class III antiarrhythmic functions.
Xu
D H
DH
Department of Pharmacy, Zhongshan University, Guangzhou, China.
Xu
S B
SB
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D001145:Arrhythmias, Cardiac / Q000235:genetics*; D051668:Delayed Rectifier Potassium Channels; D005260:Female; D006801:Humans; D009865:Oocytes / Q000378:metabolism; D015221:Potassium Channels / Q000187:drug effects / Q000235:genetics*; D024642:Potassium Channels, Voltage-Gated; D051672:Shal Potassium Channels; D014981:Xenopus / Q000235:genetics*
D000889:Anti-Arrhythmia Agents; D051668:Delayed Rectifier Potassium Channels; C497018:KCND2 protein, human; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051672:Shal Potassium Channels