title: Effect of sleep deprivation on insulin sensitivity and cortisol concentration in healthy subjects.
abstract: The objective of this study was to assess insulin sensitivity and cortisol concentration in healthy subjects with 24-hr sleep deprivation. A randomised, single-blind, controlled clinical trial was performed in 28 healthy subjects. Fourteen individuals were studied before and after 24-hr sleep deprivation and 14 volunteers with normal sleep periods (NSP) as a control group. Serum creatinine, uric acid, total cholesterol, high-density lipoprotein cholesterol, and triglyceride concentrations were measured in both groups. Insulin suppression test modified with octreotide (IST) and cortisol levels were performed before and after 24-hr sleep deprivation or NSP. Clinical and metabolic characteristics of the subjects in both groups are similar. Steady-state glucose (SSG) concentration of the IST was significantly higher after 24-hr sleep deprivation (5.7+/-2.1 vs 6.7+/-2.2 mmol/l; p=0.01). SSG level was similar before and after NSP (5.0+/-2.1 vs 5.0+/-1.8 mmol/l, respectively; p=0.91). There were not significant differences in cortisol levels between initial and final tests in both groups. In conclusion, 24-hr sleep deprivation decreased the insulin sensitivity in healthy subjects without changes in cortisol levels.
authors.affiliation: Medical Research Unit in Clinical Epidemiology, West National Medical Center, Mexican Institute of Social Security, Guadalajara City. uiec@prodigy.net.mx
authors.forename: M
authors.initials: M
authors.lastname: González-Ortiz
authors.forename: E
authors.initials: E
authors.lastname: Martínez-Abundis
authors.forename: B R
authors.initials: BR
authors.lastname: Balcázar-Muñoz
authors.forename: S
authors.initials: S
authors.lastname: Pascoe-González
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D003404:Creatinine / Q000097:blood; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology*; D008055:Lipids / Q000097:blood; D008297:Male; D016037:Single-Blind Method; D012892:Sleep Deprivation / Q000097:blood / Q000503:physiopathology*; D014527:Uric Acid / Q000097:blood
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008055:Lipids; D014527:Uric Acid; D003404:Creatinine; D006854:Hydrocortisone