The nonenzymatic hydrolysis of oligoribonucleotides. VII. Structural elements affecting hydrolysis.
Several elements of oligoribonucleotide structure are important for efficient hydrolysis. We have found that the following factors influence oligoribonucleotide hydrolysis: (i) single-stranded structure of RNA flanking the scissile phosphodiester bond, (ii) the substituent on atom C-5 of the uridine adjacent to the cleaved internucleotide bond, (iii) the position of the scissile UA phosphodiester bond within a hairpin loop, (iv) the concentration of formamide, urea, ethanol and sodium chloride.
Bibillo
A
A
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Figlerowicz
M
M
Ziomek
K
K
Kierzek
R
R
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004277:DNA, Single-Stranded / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes; D009843:Oligoribonucleotides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D014529:Uridine / Q000737:chemistry
D004277:DNA, Single-Stranded; D009692:Nucleic Acid Heteroduplexes; D009843:Oligoribonucleotides; D012313:RNA; D014529:Uridine