title: SEK1/MKK4, c-Jun and NFKappaB are differentially activated in forebrain neurons during postnatal development and injury in both control and p75NGFR-deficient mice.
abstract: The common neurotrophin receptor (p75NGFR) can signal in vitro through activation of the c-Jun N-terminal kinase (JNK) pathway and nuclear translocation of NFKappaB. Activation of JNK and its substrate c-Jun can lead to apoptosis. We investigated these activities in vivo by comparing immunoreactivity for phosphorylated(p) SEK-1 (or MKK4, which activates JNK), c-Jun (ser63, ser73) and nuclear translocation of NFKappaB-p50 in tissue sections through the forebrain of control and p75NGFR-deficient mice. During postnatal development, SEK1p-immunoreactivity was detectable in p75NGFR-positive cholinergic neurons and p75NGFR-negative neurons throughout the forebrain in control mice. During development, few cells contained c-Junp, although many neurons contained c-Jun. No obvious c-Jun immunostaining was present in the adult forebrain. At any age, NFKappaB-p50 immunoreactivity was seen in nuclei of most cells throughout the forebrain. Following fimbria fornix transection in adult mice, few basal forebrain neurons contained SEK1p while many axotomized choline acetyltransferase (ChAT)-positive neurons contained c-Junp and nuclear NFKappaB-p50. The immunostaining patterns of SEK1p, c-Junp and NFKB during development and following injury were largely similar in p75NGFR-deficient mice. During development, cells throughout the forebrain had TdT-mediated dUTP-biotin nick end labelling (TUNEL)-labelling (a potential marker for apoptosis), however, their presence was not predicted by number of neurons stained for SEK1p or c-Junp. These results suggest that the expected activation of the JNK pathway by p75NGFR, as well as the expected relationship between SEK1 and downstream activation of c-Jun do not occur in the mammalian forebrain. Also, these results suggest that this activation does not necessarily lead to cell death.
authors.affiliation: Department of Anatomy and Neurobiology, Dalhousie University, Halifax, Novia Scotia, B3H 4H7 Canada.
authors.forename: N L
authors.initials: NL
authors.lastname: Ward
authors.forename: T
authors.initials: T
authors.lastname: Hagg
mesh_terms: D000367:Age Factors; D000818:Animals; D000906:Antibodies; D019771:Axotomy; D016923:Cell Death / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002799:Cholinergic Fibers / Q000737:chemistry / Q000201:enzymology; D053938:DNA Fragmentation; D003714:Denervation; D006624:Hippocampus / Q000166:cytology / Q000254:growth & development; D020287:In Situ Nick-End Labeling; D048670:MAP Kinase Kinase 4; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D020929:Mitogen-Activated Protein Kinase Kinases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D009410:Nerve Degeneration / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D016548:Prosencephalon / Q000166:cytology / Q000254:growth & development; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D020800:Receptor, Nerve Growth Factor / Q000235:genetics*; D012686:Septal Nuclei / Q000166:cytology / Q000254:growth & development / Q000601:surgery; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000906:Antibodies; D016328:NF-kappa B; D016755:Proto-Oncogene Proteins c-jun; D020800:Receptor, Nerve Growth Factor; D002795:Choline O-Acetyltransferase; D048670:MAP Kinase Kinase 4; C482562:Map2k4 protein, mouse; D020929:Mitogen-Activated Protein Kinase Kinases