Antioxidant activity of various fractions of non-tannin phenolics of canola hulls.
Cyclone canola hulls were extracted with 70% (v/v) acetone. The dried crude extract was dissolved in ethanol and fractionated on a Sephadex LH-20 column using 95% (v/v) ethanol as the mobile phase. Five major fractions were isolated according to the UV absorption. All fractions exhibited marked antioxidant activity in a beta-carotene-linoleate model system. Fractions I and II showed the best preventive effect against the bleaching of beta-carotene. The scavenging effect of fractions I, III, and V, at 1 mg, on alpha, alpha-diphenyl-beta-picrylhydrazyl (DPPH) radical was 67.4%, 80.7%, and 63.3%, respectively. Fractions II and IV showed weak DPPH scavenging effects. The reducing power of phenolics present in fractions IV and V was greater than that of fractions I-III, and the observed data correlated well (r(2) = 0.937; P = 0.007) with the total content of phenolics present in each fraction.
Amarowicz
R
R
Department of Human Nutrition, St. Francis Xavier University, Antigonish, P.O. Box 5000, NS B2G 2W5, Canada.
Naczk
M
M
Shahidi
F
F
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D015764:Bepridil / Q000031:analogs & derivatives / Q000378:metabolism; D001713:Biphenyl Compounds; D019607:Brassicaceae / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D005609:Free Radicals; D007202:Indicators and Reagents; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010851:Picrates; D013634:Tannins; D014466:Ultraviolet Rays
D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D007202:Indicators and Reagents; D010636:Phenols; D010851:Picrates; D013634:Tannins; D015764:Bepridil; C004931:1,1-diphenyl-2-picrylhydrazyl