title: In vitro and ex vivo anti- and prooxidant components of Cichorium intybus.
abstract: The water soluble antioxidant properties of Cichorium intybus var. Silvestre, whose production zone is around Chioggia, Italy, were investigated. Vegetable juices were obtained by centrifugation, and (1) filtration at 2 degrees C; (2) filtration at 25 degrees C, and stored for 3 h; (3) boiled for 30 min at 102 degrees C, and then analysed. The antioxidant properties were evaluated in vitro as antioxidant activity (AA) (model system beta-carotene-linoleic acid) and ex vivo as protective activity (PA) against rat liver cell microsome lipid peroxidation measured as 2-thiobarbituric acid-reactive substances (TBA-RS) generated by peroxide degradation. All the vegetable juices showed high but very variable AA (> 83%) and PA (> 64%). After dialysis and analysis of fractions it was shown that the vegetable contained both biological antioxidant and prooxidant compounds. The prooxidants had MW < 3000, conversely the very active antioxidants (PA = 100%) had MW > 15,000. Electrophoretic analysis revealed that the most active fraction was a complex mixture of brown components at MW > 300,000.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Pavia, Italy. gazzani@chifar.unipv.it
authors.forename: G
authors.initials: G
authors.lastname: Gazzani
authors.forename: M
authors.initials: M
authors.lastname: Daglia
authors.forename: A
authors.initials: A
authors.lastname: Papetti
authors.forename: C
authors.initials: C
authors.lastname: Gregotti
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014675:Vegetables / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances