title: Reduced fetal, placental, and amniotic fluid PTHrP in the growth-restricted spontaneously hypertensive rat.
abstract: Evidence implicates pivotal roles for parathyroid hormone-related protein (PTHrP) in stimulating cell growth and differentiation, placental calcium transport, and placental vasodilatation. As spontaneously hypertensive rat (SHR) fetuses are growth restricted compared with those of its normotensive control, the Wistar Kyoto (WKY) rat, we examined intrauterine PTHrP and total and ionic calcium concentrations in these rats. Fetal plasma PTHrP concentrations, but not total calcium concentrations, were lower in the SHR compared with WKY (P < 0.05). SHR placental concentrations of PTHrP were lower than in WKY (P < 0.03) and failed to show the increase observed in WKY near term (P < 0.05). PTHrP concentrations in amniotic fluid from SHR were not raised near term and were lower compared with WKY (P < 0.0005). The increased ionic calcium concentrations in amniotic fluid in the WKY near term (P < 0.05) were not detected in the SHR. Thus SHR fetal plasma, placental, and amniotic fluid PTHrP concentrations were reduced and associated with fetal growth restriction. We suggest that PTHrP may play a role in the etiology of both growth restriction during pregnancy and hypertension later in life.
authors.affiliation: Department of Physiology, The University of Melbourne, Victoria 3010, Australia 3065. m.wlodek@physiology.unimelb.edu.au
authors.forename: M E
authors.initials: ME
authors.lastname: Wlodek
authors.forename: K T
authors.initials: KT
authors.lastname: Westcott
authors.forename: P W
authors.initials: PW
authors.lastname: Ho
authors.forename: A
authors.initials: A
authors.lastname: Serruto
authors.forename: R
authors.initials: R
authors.lastname: Di Nicolantonio
authors.forename: W
authors.initials: W
authors.lastname: Farrugia
authors.forename: J M
authors.initials: JM
authors.lastname: Moseley
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000097:blood; D005321:Extraembryonic Membranes / Q000033:anatomy & histology / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005317:Fetal Growth Retardation / Q000378:metabolism*; D005865:Gestational Age; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D009929:Organ Size; D044162:Parathyroid Hormone-Related Protein; D010920:Placenta / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014599:Uterus / Q000737:chemistry / Q000378:metabolism
chemical_list: D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D002118:Calcium