[Assessment of malaria DNA vaccines in mice and monkeys].
Cholera toxin B subunit is a good carrier protein and an effective adjuvant which can boost both cellular and humoral immunity. DNA fragments encoding B cell, Th cell and CTL epitopes of P. falciparum CS, MSA-1, MSA-2 and RESA antigens were cloned down-2 stream of cholera toxin B subunit gene in the same reading frame. High titer of anti-malaria epitopes antibodies and strong cellular immunogenicity were elicited after Balb/c mice were immunized three times with 100 micrograms recombinant plasmid DNA dissolved in 100 microliters PBS. A total of 120 vaccinees were challenged with mouse Plasmodium yoelli to investigate if cross protection existed. The protective efficacy was about 60%-80%. Four rhesus monkeys were challenged with 10(8) of P. cynomalgi, better results were obtained in the groups immunized with mixed plasmids including NANP, AWTE.
Zhong
H
H
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing, China.
Cao
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C
Li
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P
Li
J Z
JZ
Ma
Q J
QJ
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000913:Antibodies, Protozoan / Q000097:blood; D002772:Cholera Toxin / Q000276:immunology; D005260:Female; D008253:Macaca mulatta; D008288:Malaria / Q000517:prevention & control; D017780:Malaria Vaccines / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*
D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan; D017780:Malaria Vaccines; D019444:Vaccines, DNA; D002772:Cholera Toxin