Cytotoxic and DNA interaction activities of extracts from medicinal plants used in Argentina.
Eight crude extracts from seven Argentine plants with cancer-related ethnobotanical uses have been subjected to a bioscreening study to detect cytotoxic activity. The plants studied were: Aristolochia triangularis, Baccharis grisebachii, Bolax gummifera, Eupatorium hecatanthum, Erythrina crista-galli, Pterocaulon polystachium and Salpichroa origanifolia. Crown gall tumour inhibition, DNA interaction and cytotoxicity towards KB cells were assayed using the potato disc, the DNA-methyl green (DNA-MG) and the KB cells cytotoxicity bioassays respectively. The results obtained indicate that A. triangularis (ED50=47 microg/ml), B. gummifera (ED50=32 microg/ml) and E. hecatanthum (ED50=35 microg/ml) contained cytotoxic compounds against KB cells. All of the plants studied inhibited the growth of crown gall tumours, showing correlation between the experimental data and the uses reported for these plants. Moreover, the results obtained for the extracts of E. hecatanthum and P. polystachium indicate the presence of compounds that interact with DNA (48 and 22% of absorbance decrease, respectively). The results obtained suggest that cytotoxicity could play an important role in the activities claimed for the plants under study.
Mongelli
E
E
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Pabellón 2, 1428, Buenos Aires, Argentina.
Pampuro
S
S
Coussio
J
J
Salomon
H
H
Ciccia
G
G
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001118:Argentina; D004247:DNA / Q000187:drug effects*; D019448:Ethnobotany; D006801:Humans; D007624:KB Cells; D008739:Methyl Green; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D012394:Rosaniline Dyes; D011198:Solanum tuberosum
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D012394:Rosaniline Dyes; D008739:Methyl Green; D004247:DNA