Genetic analysis of sliding motility in Mycobacterium smegmatis.
A screen for nonsliding mutants of Mycobacterium smegmatis yielded 20 mutants with transposon insertions in the mps gene, which is involved in glycopeptidolipid biosynthesis. One mutant had an insertion in a gene predicted to encode a membrane transport protein. All mutants lacked glycopeptidolipids and were unable to form biofilms on polyvinyl chloride.
Recht
J
J
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Martínez
A
A
Torello
S
S
Kolter
R
R
D018441:Biofilms; D002352:Carrier Proteins / Q000235:genetics*; D002633:Chemotaxis / Q000235:genetics*; D004251:DNA Transposable Elements; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D020102:Mycobacterium smegmatis / Q000235:genetics*; D016366:Open Reading Frames; D011143:Polyvinyl Chloride
D002352:Carrier Proteins; D004251:DNA Transposable Elements; D008565:Membrane Proteins; D011143:Polyvinyl Chloride