Use of restriction fragment length polymorphism to distinguish between salmon species.
Identification of 10 salmon species using DNA-based methodology was investigated. Amplification of DNA was carried out using a primer set which amplified a region of the mitochondrial cytochrome b gene. Sequences of PCR-amplified DNA from the salmon species were used to select six restriction enzymes allowing species to be uniquely classified. RFLP patterns generated following analysis with each enzyme were resolved using polyacrylamide gel electrophoresis and visualized by silver staining. Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.
Russell
V J
VJ
Rowett Research Institute, Aberdeen, Scotland, UK.
Hold
G L
GL
Pryde
S E
SE
Rehbein
H
H
Quinteiro
J
J
Rey-Mendez
M
M
Sotelo
C G
CG
Pérez-Martin
R I
RI
Santos
A T
AT
Rosa
C
C
D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D017683:Oncorhynchus / Q000145:classification / Q000235:genetics; D017685:Oncorhynchus keta / Q000145:classification / Q000235:genetics; D017684:Oncorhynchus kisutch / Q000145:classification / Q000235:genetics; D017686:Oncorhynchus mykiss / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012474:Salmon / Q000145:classification / Q000235:genetics; D012487:Salmonidae / Q000145:classification* / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D014337:Trout / Q000145:classification / Q000235:genetics