title: A rapid LC method for the identification and determination of CNS drugs in pharmaceutical formulations.
abstract: Antidepressant, neuroleptic and antiepileptic drugs were identified and determined in pharmaceutical formulations (tablets, capsules and oral solutions) by a rapid high-performance liquid chromatography method. The sample pretreatment consisted of a one-step extraction, filtration and dilution. The chromatographic conditions were: reversed-phase C8 column (150 x 4.6 mm i.d., 5 microm); acetonitrile-tetramethylammonium perchlorate aqueous solution (pH 2.8; 12.6 mM) (45:55, v/v) as the mobile phase; detection wavelength, 230 nm. Calibration curves were linear in the 100-1000 ng ml(-1) range for all tested drugs except for phenobarbital. The repeatability (or intra-day precision), expressed by the relative standard deviation, was better than 2.0%. The accuracy, resulting from recovery studies, was between 98.1 and 101.3%. The amount of drug found agreed with the declared content within the limits specified by United States Pharmacopeia and British Pharmacopeia.
authors.affiliation: Department of Pharmaceutical Sciences, University of Bologna, Italy. raggima@kaiser.alma.unibo.it
authors.forename: M A
authors.initials: MA
authors.lastname: Raggi
authors.forename: G
authors.initials: G
authors.lastname: Casamenti
authors.forename: R
authors.initials: R
authors.lastname: Mandrioli
authors.forename: C
authors.initials: C
authors.lastname: Sabbioni
authors.forename: V
authors.initials: V
authors.lastname: Volterra
mesh_terms: D002138:Calibration; D002491:Central Nervous System Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002491:Central Nervous System Agents; D004364:Pharmaceutical Preparations