The identification of a nonclassical cadherin expressed during B cell development and its interaction with surrogate light chain.
A 130-kDa glycoprotein (p130) has been found to be associated with surrogate light chain on pro- and pre-B I cells. Using peptide sequences obtained from purified p130 we have cloned its gene. The gene encodes a typical cadherin type 1 membrane protein with six extracellular cadherin domains (one pseudo domain) but lacking the catenin-binding site in its cytoplasmic part. Even without this catenin-binding site, p130 mediates Ca(2+)-dependent homotypic adhesion of cells. The interaction of p130 with surrogate light chain is confirmed by co-transfection and co-immunoprecipitation experiments. The expression of p130 is biphasic during the B cell development. Reverse transcriptase-polymerase chain reaction and flow cytometric analyses revealed that it is expressed on B220(+)c-Kit(+) pro-B and pre-B-I cells as well as on B220(+)CD25(-)IgM(+) immature and mature B cells but not on B220(+)CD25(+) pre-B-II cells. It is also expressed in fetal liver, at low levels in myeloid cells, and strongly in intestinal epithelial cells. In the spleen, p130-expressing cells are mainly localized in the marginal zone. We call this B lineage-, intestine-, liver- and leukocyte-expressed gene BILL-cadherin. The possible functions of BILL-cadherin in B cell development are discussed.
Ohnishi
K
K
Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland and the Department of Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan. ohnishik@nih.go.jp
Shimizu
T
T
Karasuyama
H
H
Melchers
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D015820:Cadherins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D005260:Female; D005434:Flow Cytometry; D015723:Gene Library; D007147:Immunoglobulin Light Chains / Q000737:chemistry; D054444:Immunoglobulin Light Chains, Surrogate; D007075:Immunoglobulin M / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D017434:Protein Structure, Tertiary; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D051381:Rats; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014018:Tissue Distribution; D014162:Transfection
D000911:Antibodies, Monoclonal; D015820:Cadherins; C490585:Cdh17 protein, mouse; D018076:DNA, Complementary; D007147:Immunoglobulin Light Chains; D054444:Immunoglobulin Light Chains, Surrogate; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D008562:Membrane Glycoproteins; D015375:Receptors, Interleukin-2; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D002118:Calcium