Volume expansion sensitivity of swelling-activated Cl(-) channel in human epithelial cells.
In human epithelial cell lines, whole-cell swelling-activated Cl(-) current was not directly correlated to cell volume per se, membrane tension or hydrostatic pressure. The current density exhibited a relation described by a Boltzmann function to the square of the cell diameter. Cytochalasin D enhanced the volume sensitivity. These results suggest that the activation mechanism of volume-sensitive Cl(-) channel is related to cytoskeleton-dependent membrane spring energy in human epithelial cells.
Morishima
S
S
Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, 444-8585 Japan.
Shimizu
T
T
Kida
H
H
Okada
Y
Y
D002460:Cell Line; D048429:Cell Size / Q000502:physiology; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000502:physiology*; D017136:Ion Transport / Q000502:physiology
D018118:Chloride Channels; D002712:Chlorides