title: Polymorphisms of HLA DQB1 CAR1/CAR2 and TNFalpha IR2/IR4 microsatellite markers in patients affected with Graves disease.
abstract: Graves disease is an autoimmune thyroid disorder in which HLA class I and II confer susceptibility in different ethnic groups. An allele-specific polymerase chain reaction was used to characterize the variation of the HLA-DQB1 CAR1/CAR2 and TNFalpha markers located on chromosome 6 in 85 unrelated Tunisian patients affected with Graves disease and 148 healthy control subjects. In contrast to the results regarding TNFalpha alleles showing no difference between patients and controls (P = 0.904), the analysis of the HLA-DQB1 CAR1/CAR2 polymorphism showed a significant difference in its alleles (P < 0.0001). Nevertheless, no allele exhibited a predispositional effect on GD pathogenesis.
authors.affiliation: Faculté de Médecine, Laboratoire de Génétique Moléculaire Humaine, Sfax, 3028, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Maalej
authors.forename: H
authors.initials: H
authors.lastname: Hadj Kacem
authors.forename: M
authors.initials: M
authors.lastname: Bellassoued
authors.forename: M
authors.initials: M
authors.lastname: Abid
authors.forename: H
authors.initials: H
authors.lastname: Makni
authors.forename: H
authors.initials: H
authors.lastname: Ayadi
mesh_terms: D000483:Alleles; D006111:Graves Disease / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D018895:Microsatellite Repeats / Q000502:physiology; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D014416:Tunisia
chemical_list: D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D014409:Tumor Necrosis Factor-alpha