Decrease of hindpaw withdrawal latency by cocaine- and amphetamine-regulated transcript peptide to the mouse spinal cord.
Immunohistochemical studies with the use of an antiserum against the cocaine- and amphetamine-regulated transcript (CART) peptide-(55-102) showed an abundance of CART-immunoreactive fibers in the mouse dorsal horn laminae I and II. A few CART-positive somata were scattered in the dorsal horn and around the central canal. Intrathecal injection of the CART peptide-(55-102) at doses 3, 10 and 100 ng caused a dose-dependent and significant decrease of paw withdrawal latency; whereas, saline injection was without significant effect. Our results provide the first evidence that CART-immunoreactive fibers are present in the dorsal horn and that the peptide administered intrathecally produces hyperalgesia, as assessed by paw withdrawal latency in mice.
Ohsawa
M
M
Department of Anesthesiology, Medical College of Wisconsin, Watertown Plank Road, Milwaukee, WI 52226, USA.
Dun
S L
SL
Tseng
L F
LF
Chang
J
J
Dun
N J
NJ
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006614:Hindlimb; D007150:Immunohistochemistry; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010146:Pain / Q000503:physiopathology / Q000517:prevention & control*; D010147:Pain Measurement; D010446:Peptide Fragments / Q000494:pharmacology; D011930:Reaction Time / Q000187:drug effects; D013116:Spinal Cord / Q000737:chemistry; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein