title: Cytokine production by in vitro processed and unprocessed haematopoietic cells.
abstract: The studies described here demonstrate that in vitro processing of cells before extraction of RNA has a major effect on the number and type of cytokine transcripts present within MDS and leukemia cells. Transcripts for GM-CSF, a cytokine whose production by leukemia cells is believed to play an important role in the pathogenesis of leukemia, was not detectable in 12/13 unprocessed AML specimens, in 12/12 MDS specimens, or in 7/7 CML specimens but once detected in many specimens after processing. These data strongly suggest that leukemia cell production of GMCSF rarely occurs in vivo.
authors.affiliation: Rush Cancer Institute, Departments of Medicine, Immunology & Microbiology, Rush-Presbyterian-St Luke's Medical Center, Rush Medical College, Chicago, IL 60612, USA. sbi@rush.edu
authors.forename: S
authors.initials: S
authors.lastname: Bi
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.forename: E
authors.initials: E
authors.lastname: Devemy
authors.forename: H
authors.initials: H
authors.lastname: Chopra
authors.forename: P
authors.initials: P
authors.lastname: Venugopal
authors.forename: A
authors.initials: A
authors.lastname: Raza
authors.forename: H D
authors.initials: HD
authors.lastname: Preisler
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D018929:Cell Culture Techniques; D002469:Cell Separation; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000276:immunology; D013048:Specimen Handling; D020298:U937 Cells
chemical_list: D016207:Cytokines