Using microwave distillation-solid-phase microextraction--gas chromatography--mass spectrometry for analyzing fish tissue.
A technique for the analysis of the volatile compounds from fish tissue employing microwave distillation-solid-phase microextraction-gas chromatography-mass spectrometry is described. A qualitative listing of 174 compounds observed in the headspace is given, and a quantitative method for the determination of the off-flavor contaminants (2-methylisoborneol and geosmin) is presented. Borneol and decahydro-1-naphthol are used as the surrogate and internal standards, respectively. A linear calibration curve is obtained for 0.1 to 5 ppb with a recovery level of 60% at 2.5 ppb. Comparison of the instrumental method with a human flavor checker showed good agreement.
Grimm
C C
CC
USDA-ARS-SRRC, New Orleans, LA 70124, USA. cgrimm@nola.srrc.usda.gov
Lloyd
S W
SW
Batista
R
R
Zimba
P V
PV
D000818:Animals; D002397:Catfishes; D005396:Fish Products / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008872:Microwaves; D012015:Reference Standards