Detection of prognostic significant translocations in childhood acute lymphoblastic leukaemia by one-step multiplex reverse transcription polymerase chain reaction.
The search for chromosomal translocations in de novo cases of childhood acute lymphoblastic leukaemia (ALL) is crucial for the selection of the appropriate therapeutic protocol. In this work, we describe a new method - one-step multiplex reverse transcription polymerase chain reaction (RT-PCR) - to screen for prognostic significant translocations in childhood ALL. Our approach involves a single PCR reaction for the simultaneous detection of the molecular rearrangements resulting from the t(9;22), t(12;21), t(4;11) and t(1;19), with a turnaround time of less than 24 h. This assay proved to be highly sensitive, specific, reproducible and easy to implement in a routine genetics laboratory.
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Unidade de Oncologia Molecular, Serviço de Genética, Instituto Português de Oncologia, Porto, Portugal.
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Mariz
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Marques
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D002675:Child, Preschool; D002878:Chromosomes, Human, Pair 1; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D002888:Chromosomes, Human, Pair 19; D002891:Chromosomes, Human, Pair 21; D002892:Chromosomes, Human, Pair 22; D002894:Chromosomes, Human, Pair 4; D002899:Chromosomes, Human, Pair 9; D006801:Humans; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics*; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014178:Translocation, Genetic