Basic fibroblast growth factor inhibits cell proliferation in cultured avian inner ear sensory epithelia.
Postembryonic production of inner ear hair cells occurs after insult in nonmammalian vertebrates. Recent studies suggest that the fibroblast family of growth factors may play a role in stimulating cell proliferation in mature inner ear sensory epithelium. Effects of acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) were tested on progenitor cell division in cultured auditory and vestibular sensory epithelia taken from posthatch chickens. The effects of heparin, a glycosaminoglycan that often potentiates the effects of the FGFs, were also assessed. Tritiated-thymidine autoradiographic techniques and 5-bromo-2;-deoxyuridine (BrdU) immunocytochemistry were used to identify cells synthesizing DNA. The terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick-end-label (TUNEL) method was used to identify apoptotic cells. TUNEL and overall counts of sensory epithelial cell density were used to assess possible cytotoxic effects of the growth factors. FGF-2 inhibited DNA synthesis in vestibular and auditory sensory epithelia and was not cytotoxic at the concentrations employed. FGF-1 did not significantly alter sensory epithelial cell proliferation. Heparin by itself inhibited DNA synthesis in the vestibular sensory epithelia and failed to potentiate the effects of FGF-1 or FGF-2. Heparin was not cytotoxic at the concentrations employed. Results presented here suggest that FGF-2 may be involved in inhibiting cell proliferation or stimulating precursor cell differentiation in avian inner ear sensory epithelia.
Oesterle
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Virginia Merrill Bloedel Hearing Research Center and Department of Otolaryngology-HNS, University of Washington, Seattle, Washington 98195-7923, USA. oesterle@u.washington.edu
Bhave
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Coltrera
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D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001798:Blood Proteins / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects* / Q000502:physiology; D002645:Chickens / Q000033:anatomy & histology / Q000378:metabolism*; D007758:Ear, Inner / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects / Q000502:physiology; D012444:Saccule and Utricle / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology
D001798:Blood Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D006493:Heparin