Activation of phospholipase D activity in transforming growth factor-beta-induced cell growth inhibition.
Cells regulate phospholipase D (PLD) activity in response to numerous extracellular signals. Here, we investigated the involvement of PLD activity in transforming growth factor-beta (TGF-beta1)-mediated growth inhibition of epithelial cells. TGF-beta1 inhibits the growth of MDCK, Mv1Lu, and A-549 cells. In the presence of 0.4% butanol, TGF-beta1 induces an increase in the formation of phosphatidylbutanol, a unique product catalyzed by PLD. TGF-beta1 also induces an increase in phosphatidic acid (PA) level in A-549 and MDCK cells. TGF-beta1 induces an increase in the levels of DAG labeled with [3H]-myristic acid in A-549 and MDCK cells but not in Mv1Lu cells. No increase of DAG was observed in cells prelabeled with [3H]-arachidonic acid. The data presented suggest that PLD activation is involved in the TGF-beta1-induced cell growth inhibition.
Zhou
B H
BH
State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Chinese Academy of Sciences.
Chen
J S
JS
Chai
M Q
MQ
Zhao
S
S
Liang
J
J
Chen
H H
HH
Song
J G
JG
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004075:Diglycerides / Q000096:biosynthesis; D004285:Dogs; D004789:Enzyme Activation; D006131:Growth Inhibitors / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D008907:Mink; D010713:Phosphatidylcholines / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism* / Q000494:pharmacology; D053773:Transforming Growth Factor beta1
D004075:Diglycerides; D006131:Growth Inhibitors; D010713:Phosphatidylcholines; D018129:Phosphatidylinositol Phosphates; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D010739:Phospholipase D