Functional vagal input to gastric myenteric plexus as assessed by vagal stimulation-induced Fos expression.
Immunohistochemical detection of c-Fos expression was used to identify gastric myenteric plexus neurons that receive excitatory input from vagal efferent neurons activated by electrical stimulation of the cervical vagi in anesthetized rats. Vagal stimulation-induced Fos expression increased with higher pulse frequency, so that with 16 Hz (rectangular pulses of 1 mA/0.5 ms for 30 min) approximately 30% and with 48 Hz 90% of all neurons near the lesser curvature were Fos positive. In sham-stimulated rats there was no Fos expression. The percentage of Fos-activated neurons was only slightly smaller (85% with 48 Hz) near the greater curvature. Prior atropine administration (1 mg/kg ip) had little effect on vagal stimulation-induced Fos expression, and in unilaterally stimulated rats there was no Fos expression on the contralateral (noninnervated) side of the stomach, ruling out mediation by gastric motility or secretory responses. However, polysynaptic recruitment of third- and higher-order neurons cannot be ruled out completely. These results support the idea that, at least in the stomach, functional excitatory innervation of myenteric plexus neurons by the efferent vagus is profuse and widespread, refuting the notion of only a few vagal "command neurons."
Zheng
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Neurobiology of Nutrition Laboratory, Pennington Biomedical Research Center, Louisiana State University, Baton Rouge 70808, USA.
Berthoud
H R
HR
D000759:Adjuvants, Anesthesia / Q000494:pharmacology; D000778:Anesthetics, Dissociative / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004525:Efferent Pathways; D004558:Electric Stimulation; D004594:Electrophysiology; D007150:Immunohistochemistry; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000502:physiology*; D009407:Nerve Block; D009431:Neural Conduction / Q000187:drug effects; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D010276:Parasympatholytics / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000294:innervation*; D014628:Vagotomy; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*
D000759:Adjuvants, Anesthesia; D000778:Anesthetics, Dissociative; D010276:Parasympatholytics; D016760:Proto-Oncogene Proteins c-fos; D007649:Ketamine; D001285:Atropine; D010424:Pentobarbital