Influence of various technological parameters on the preparation of spray-dried poly(epsilon-caprolactone) microparticles containing a model antigen.
This work evaluates the efficacy of the spray-drying technique to prepare poly(epsilon-caprolactone) (PCL) microparticles containing an entrapped model antigen (bovine albumin, BSA). The presence of a stabiliser was found to be an important parameter when preparing PCL microparticles containing a hydrophilic antigen. The effect of various technological parameters (concentration of the polymer and protein solutions, organic/aqueous phases ratio, nature of solvents and emulsion parameters such as duration and speed of agitation) on microparticle morphology and size, BSA entrapment and encapsulation efficiency was studied. Microparticles were characterized by a mean size from 9.56+/-0.25 to 24.31+/-2.87 microm and a BSA entrapment from 0.80+/-0.02 to 24.21+/-0.23% (w/w). SDS-PAGE electrophoresis and isoelectric focusing (IEF) confirmed the conservation of the physicochemical characteristics of the BSA entrapped within PCL microparticles produced by spray-drying. Together, these results showed that spray-drying is an efficient technique to overcome the key obstacle that represents the scaling-up of the manufacturing process to produce sufficient quantities of vaccine for clinical trials and, ultimately, commercialization.
Baras
B
B
Laboratoire de Pharmacie Galénique, Industrielle et Officinale, Ecole de Pharmacie, Université Catholique de Louvain, Brussels, Belgium. ipv@pasteur-
Benoit
M A
MA
Gillard
J
J
D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000737:chemistry; D002214:Capsules / Q000302:isolation & purification*; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004339:Drug Compounding; D004655:Emulsions; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D011091:Polyesters / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology; D012996:Solutions; D014612:Vaccines / Q000008:administration & dosage
D000941:Antigens; D002214:Capsules; D004655:Emulsions; D011091:Polyesters; D012996:Solutions; D014612:Vaccines; C016240:polycaprolactone; D012710:Serum Albumin, Bovine