Ocat, a paternally expressed gene closely linked and transcribed in the opposite direction to Peg3.
Two reciprocally imprinted Kruppel-type zinc finger genes, Peg3 and Zim1, have been found in close proximity on mouse proximal chromosome 7. Here, we describe the identification of another novel imprinted transcript, Ocat (ossification center-associated transcript). Ocat encodes a 5.5-kb spliced transcript and is transcribed in the opposite orientation to Peg3. One of its exons (putative exon 1) lies approximately 200 bp upstream of Peg3. Ocat is predominantly expressed in embryos from days 11 to 17, particularly in ossification centers of the embryonic skeleton at day 15. Like Peg3, Ocat is expressed exclusively from the paternal allele. Despite their proximity, Peg3 and Ocat showed dissimilar expression profiles, suggesting that they are regulated by independent genetic elements.
Szeto
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IY
Wellcome/CRC Institute of Cancer and Developmental Biology, Department of Physiology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, United Kingdom. yys22@mole.bio.cam.ac.uk
Li
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LL
Surani
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MA
D000818:Animals; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage; D018392:Genomic Imprinting; D051741:Kruppel-Like Transcription Factors; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae; D011247:Pregnancy; D011494:Protein Kinases; D011506:Proteins / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic
D018076:DNA, Complementary; D051741:Kruppel-Like Transcription Factors; C097901:Peg3 protein, mouse; D011506:Proteins; D014157:Transcription Factors; D011494:Protein Kinases