Application of ion-exchange cartridge clean-up in food analysis III. Determination of benzylpenicillin, phenoxymethyl-penicillin, oxacillin, cloxacillin, nafcillin and dicloxacillin in bovine liver and kidney by liquid chromatography with ultraviolet detection.
A multiresidue analytical method was developed for the simultaneous determination of benzylpenicillin (PCG), phenoxymethylpenicillin (PCV), oxacillin (MPIPC), cloxacillin (MCIPC), nafcillin (NFPC) and dicloxacillin (MDIPC) in bovine liver and kidney. The method involves the use of an ion-exchange cartridge for sample clean-up followed by ion-pair high-performance liquid chromatography with ultraviolet detection. The recoveries of PCG, PCV, MPIPC, MCIPC, NFPC and MDIPC from bovine liver spiked at levels of 0.5 mg/kg and 0.1 mg/kg were in the range of 73-91% and 83-96% with coefficients of variation of 1.4-4.2% and 3.4-8.7%, respectively. For bovine kidney spiked at levels of 0.5 mg/kg and 0.1 mg/kg, the recoveries of these compounds were 79-92% and 82-92% with RSDs of 1.8-5.9% and 2.7-7.8%, respectively. The detection limits for the six penicillins were 0.02-0.05 mg/kg in bovine liver and kidney.
Ito
Y
Y
Aichi Prefectural Institute of Public Health, Nagoya, Japan.
Ikai
Y
Y
Oka
H
H
Matsumoto
H
H
Kagami
T
T
Takeba
K
K
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D010406:Penicillins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D010406:Penicillins