A novel endoproteolytic processing activity in mitochondria of erythroid cells and the role in heme synthesis.
The erythroid isoform of aminolevulinate synthase (eALAS) protein is a major control point in erythroid heme synthesis and hemoglobin formation. Erythroid cells were extracted from mouse blood and bone marrow and metabolically labeled with (35)S-methionine. This was followed by immunoprecipitation of eALAS protein products. The results show that the N-terminus of the expected full-length 59-kd form of the eALAS protein is truncated in bone marrow erythroid cells by approximately 7 kd. More differentiated erythroid cells in the peripheral blood exhibit very little of this protein truncation. Erythroid cells from the bone marrow were isolated using monoclonal antibody TER-119 and were shown to contain a unique endoprotease activity that could cleave the eALAS protein to the shorter form in vitro. With or without the mitochondrial signal sequence, the eALAS protein could serve as a substrate for the cleavage. This cleavage renders a functional eALAS protein and only removes a domain of unclear function, which has previously been reported to vary in size as a result of alternative RNA splicing. The protease activity was enriched from the membranes of mitochondria from bone marrow cells and was shown to be different from mitochondrial processing peptidase, medullasin, and other known proteases. Apart from the mitochondrial processing peptidase that cleaves the import signal sequence, this is the first description of a mitochondrially located site-specific processing protease activity. (Blood. 2000;96:740-746)
Dzikaite
V
V
Microbiology and Tumorbiology Center, Karolinska Institutet, Stockholm, Sweden.
Kanopka
A
A
Brock
J H
JH
Kazlauskas
A
A
Melefors
O
O
D000624:5-Aminolevulinate Synthetase / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002469:Cell Separation; D010450:Endopeptidases / Q000378:metabolism*; D004912:Erythrocytes / Q000648:ultrastructure*; D015672:Erythroid Precursor Cells / Q000648:ultrastructure; D006418:Heme / Q000096:biosynthesis*; D006801:Humans; D018189:Immunomagnetic Separation; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013462:Sulfur Radioisotopes
D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D006418:Heme; D000624:5-Aminolevulinate Synthetase; D010450:Endopeptidases