title: Identification of a cDNA encoding an isoform of human CTP synthetase.
abstract: A full-length cDNA clone encoding an isoform of human CTP synthetase (type II) was isolated. A 1761-nucleotide open reading frame which corresponds to a protein of 586 amino acids with a predicted molecular mass of 65678 Da was identified. The predicted protein sequence showed 74% identity with the translation product of a previously identified human CTP synthetase cDNA clone (type I). The function of the human cDNA encoding type II CTP synthetase was verified by successful complementation of the cytidine-requiring CTP synthetase deficient mutant JF618 of Escherichia coli. The gene encoding type II CTP synthetase has been localized on chromosome Xp22.
authors.affiliation: Academic Medical Center, University of Amsterdam, Emma Children's Hospital and Department of Clinical Chemistry, Laboratory Genetic Metabolic Diseases, PO Box 22700, 1100 DE, Amsterdam, The Netherlands. a.b.vankuilenburg@amc.uva.nl
authors.forename: A B
authors.initials: AB
authors.lastname: van Kuilenburg
authors.forename: R
authors.initials: R
authors.lastname: Meinsma
authors.forename: P
authors.initials: P
authors.lastname: Vreken
authors.forename: H R
authors.initials: HR
authors.lastname: Waterham
authors.forename: A H
authors.initials: AH
authors.lastname: van Gennip
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019731:Carbon-Nitrogen Ligases / Q000235:genetics*; D002874:Chromosome Mapping; D018076:DNA, Complementary; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D014960:X Chromosome
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D019731:Carbon-Nitrogen Ligases; C019951:CTP synthetase