Functional significance of prop-1 gene expression in pituitary adenomas.
Prophet of Pit-1 (Prop-1), which is a paired-like homeodomain transcription factor, is capable of binding to sites in an early enhancer of the Pit-1 gene and regulating its expression. As human Pit-1 is expressed considerably in pituitary adenomas, we studied human Prop-1 gene expression in pituitary adenomas. We also sequenced the Prop-1 cDNAs in pituitary adenomas. Human Prop-1 transcript was detected in all pituitary adenomas examined by RT-PCR analysis. The expression of human Prop-1 in pituitary adenomas was confirmed by in situ hybridization in one of the GH-producing adenomas. The sequence analysis of human Prop-1 cDNAs in these pituitary adenomas revealed that there were no mutations except 5 silent nucleic acid substitutions, suggesting that mutations of Prop-1 gene do not represent a frequent mechanism of human pituitary tumorigenesis.
Usui
T
T
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.
Nakamura
Y
Y
Mizuta
H
H
Murabe
H
H
Muro
S
S
Suda
M
M
Tanaka
K
K
Tanaka
I
I
Shimatsu
A
A
Nakao
K
K
D000236:Adenoma / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D001483:Base Sequence / Q000235:genetics; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D010911:Pituitary Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018398:Homeodomain Proteins; C102134:Prophet of Pit-1 protein