title: Genomic organization and characterization of human PEX2 encoding a 35-kDa peroxisomal membrane protein.
abstract: Peroxins are proteins involved in peroxisome biogenesis and are encoded by PEX genes. The human PEX2 gene encodes a 35-kDa peroxisomal integral membrane protein which is a member of the zinc finger protein family. Mutations in the PEX2 gene are the primary defect in a subset of patients with Zellweger syndrome and related peroxisome biogenesis disorders. The role of zinc finger proteins in peroxisome assembly and function is poorly understood. Here we report the cloning and characterisation of the human PEX2 structural gene. PEX2 was assigned to human chromosome 8q13-q21 and its murine homologue to mouse chromosome 3. The gene is approximately 17.5 kb in length, and contains four exons. The entire coding sequence is included in one exon, exon 4. The 5'-flanking region has features of housekeeping genes (GC enrichment, two Sp1 sites) and tissue-specific, inducible genes (two CCAAT boxes). In more than 1.5 kb of 5'-flanking sequences we did not identify consensus peroxisomal proliferator responsive elements (PPRE).
authors.affiliation: Department of Paediatrics, Heinrich Heine University Düsseldorf, Moorenstrasse 5, Düsseldorf, D-40225, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Biermanns
authors.forename: J
authors.initials: J
authors.lastname: Gärtner
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D000074428:Peroxisomal Biogenesis Factor 2; D011401:Promoter Regions, Genetic
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; C000614230:PEX2 protein, human; C000614231:PEX2 protein, mouse; D000074428:Peroxisomal Biogenesis Factor 2