Induction of quinone reductase and glutathione transferase in rat tissues by juglone and plumbagin.
The ability of the naturally-occurring naphthoquinone derivatives, juglone and plumbagin, to increase tissue activities of the Phase II detoxification enzymes quinone reductase (QR) and glutathione transferase (GT) has been investigated in rats. Groups of female Sprague-Dawley rats were dosed by oral intubation on 5 consecutive days with either juglone or plumbagin at 12.5, 25, 50, 75, 100 or 125 mumoles/kg/day. The animals were then killed and the activities of QR and GT determined in tissue homogenates. The naphthoquinone derivatives had no significant effect on enzyme activities in the liver, spleen, heart, lung or urinary bladder. Increases in the activities of one or both enzymes were recorded, however, in the caecum, kidney, forestomach, duodenum, colon, glandular stomach and jejunum. The possibility that induction of Phase II enzymes could contribute to the previously-reported ability of juglone and plumbagin to protect animals against chemically-induced intestinal neoplasia is discussed.
Munday
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AgResearch, Ruakura Agricultural Research Centre, Hamilton, New Zealand. mundayr@agresearch.cri.nz
Munday
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D000284:Administration, Oral; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D009285:Naphthoquinones / Q000494:pharmacology*; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley
D000970:Antineoplastic Agents; D009285:Naphthoquinones; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase; C005134:juglone; C014758:plumbagin