title: Similarity of avian paramyxovirus serotype 1 isolates of low virulence for chickens obtained from contaminated poultry vaccines and from poultry flocks.
abstract: At present Denmark has the status of a 'non-vaccinating' country for Newcastle disease and its poultry population should therefore be free of antibodies to avian paramyxovirus 1 (APMV-1). Three live avian vaccines against infectious bronchitis, avian encephalomyelitis, and chick anaemia which had been found to be contaminated with APMV-1 viruses of low virulence for chickens were examined. The vaccines were produced by the same company and the affected batches had been used in Denmark in 1996/97. Furthermore, APMV-1 isolates of low virulence were obtained from three commercial broiler breeder flocks, one of which had been vaccinated with two of the contaminated vaccines. The flocks belonged to the same hatchery organisation. A comparison of viral F0 gene sequences and typing of virus isolates with a panel of monoclonal antibodies showed that the vaccine and field isolates were identical.
authors.affiliation: Danish Veterinary Laboratory, Aarhus.
authors.forename: P H
authors.initials: PH
authors.lastname: Jørgensen
authors.forename: K J
authors.initials: KJ
authors.lastname: Handberg
authors.forename: P
authors.initials: P
authors.lastname: Ahrens
authors.forename: R J
authors.initials: RJ
authors.lastname: Manvell
authors.forename: K M
authors.initials: KM
authors.lastname: Frost
authors.forename: D J
authors.initials: DJ
authors.lastname: Alexander
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002645:Chickens; D003718:Denmark; D004340:Drug Contamination; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000276:immunology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping / Q000662:veterinary; D014765:Viral Vaccines; D014774:Virulence
chemical_list: D000911:Antibodies, Monoclonal; D014765:Viral Vaccines