Changes of transmembrane Ca2+ gradient in the formation of macrophage-derived foam cells.
Macrophages from C57BL/6J mice, an animal susceptible to atherosclerosis, were chosen as target cells to study changes in the transmembrane Ca2+ gradient during the formation of macrophage-derived foam cells. The transmembrane Ca2+ gradients of single living cells were examined-using Fura-2/AM combined with Fluo-3/AM by laser scanning confocal microscopy. Exposure to Oxidized Low Density Lipoprotein, decreases the Ca2+ gradient across macrophage plasma membrane, but increases that across the nuclear membrane. The altered transmembrane Ca2+ gradients could induce the up-regulation of scavenger receptor in macrophages, resulting in the formation of foam cells.
Yang
X
X
NCI-FCRDC, Frederick, Maryland 21702, USA.
Zhang
Y
Y
Huang
Y
Y
Yang
F
F
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003600:Cytosol / Q000378:metabolism; D005434:Flow Cytometry; D005487:Foam Cells / Q000378:metabolism*; D006801:Humans; D007477:Ions; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D015854:Up-Regulation
D007477:Ions; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D002784:Cholesterol; D002118:Calcium