[The conservative region sequence analysis of PdSox9 in Paramisgurnus dabryanus].
Degenerate primers within HMG-box analyzed the Sox genes of P. dabryanus. Three amplification bands with the length 220, 550 and 1500 bp respectively were observed. A novel gene was obtained from the 550 bp band. The identity of possible amino acid sequence to Sox9 of human, mouse and chicken is 94%. The similarity to HSOX10, MSox10, HSRY, MSry is 87%, 55%, 40% and 47% respectively. So, it was named PdSox9. Northern blotting result showed that it overexposes in adult testis and brain.
Chang
Z J
ZJ
College of Life Sciences, Wuhan University, Hubei Province, China.
Zhou
R J
RJ
Yu
Q X
QX
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D005399:Fishes / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D055758:SOX9 Transcription Factor; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D006609:High Mobility Group Proteins; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; C527933:Sox9 protein, mouse; D014157:Transcription Factors