Structure of a thioredoxin-like [2Fe-2S] ferredoxin from Aquifex aeolicus.
The 2.3 A resolution crystal structure of a [2Fe-2S] cluster containing ferredoxin from Aquifex aeolicus reveals a thioredoxin-like fold that is novel among iron-sulfur proteins. The [2Fe-2S] cluster is located near the surface of the protein, at a site corresponding to that of the active-site disulfide bridge in thioredoxin. The four cysteine ligands are located near the ends of two surface loops. Two of these ligands can be substituted by non-native cysteine residues introduced throughout a stretch of the polypeptide chain that forms a protruding loop extending away from the cluster. The presence of homologs of this ferredoxin as components of more complex anaerobic and aerobic electron transfer systems indicates that this is a versatile fold for biological redox processes.
Yeh
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Division of Chemistry and Chemical Engineering 147-75CH, California Institute of Technology, Pasadena, CA, 91125, USA.
Chatelet
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Soltis
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Kuhn
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Meyer
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Rees
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D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D001419:Bacteria / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D019281:Dimerization; D004220:Disulfides / Q000378:metabolism; D042967:Electron Transport Complex I; D005288:Ferredoxins / Q000737:chemistry* / Q000235:genetics; D007501:Iron / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008983:Molybdoferredoxin / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry; D009591:Nitrogenase / Q000378:metabolism; D011485:Protein Binding; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013455:Sulfur / Q000378:metabolism; D013879:Thioredoxins / Q000737:chemistry*
D004220:Disulfides; D005288:Ferredoxins; D008024:Ligands; D008983:Molybdoferredoxin; D013879:Thioredoxins; D013455:Sulfur; D007501:Iron; D009591:Nitrogenase; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I; D003545:Cysteine