Substance P radioimmunoassay for quantitative characterization of sensory neurotransmitter release.
In the present work we report the development of a new radioimmunoassay method for measuring the substance P content liberated from isolated rat tracheae in response to electrical or chemical (capsaicin, resiniferatoxin, piperine) stimulation. The amount of substance P released by electrical stimulation has been found to be dependent on the number of pulses and chemically elicited substance P release also proved to be dose-dependent. Our findings reinforce previous data that resiniferatoxin is approximately 100 times more potent than capsaicin and the potency ratio between piperine and capsaicin is 1/50.
Németh
J
J
Department of Pharmacology and Pharmacotherapy, Medical University of Pécs, Hungary.
Oroszi
G
G
Thán
M
M
Helyes
Z S
ZS
Pintér
E
E
Farkas
B
B
Szolcsányi
J
J
D000470:Alkaloids; D000818:Animals; D052117:Benzodioxoles; D002138:Calibration; D002211:Capsaicin / Q000494:pharmacology; D004224:Diterpenes / Q000494:pharmacology; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism*; D010880:Piperidines / Q000494:pharmacology; D053284:Polyunsaturated Alkamides; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013373:Substance P / Q000032:analysis* / Q000378:metabolism*; D014132:Trachea / Q000294:innervation*
D000470:Alkaloids; D052117:Benzodioxoles; D004224:Diterpenes; D004791:Enzyme Inhibitors; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D013373:Substance P; C024353:resiniferatoxin; D002211:Capsaicin; C008922:piperine