title: Tumor antigen presentation by dermal antigen-presenting cells.
abstract: Several phenotypes of antigen-presenting cells are present in the dermis, where they presumably function to present encountered antigens for immune responses. This study examined the ability of dermal antigen-presenting cells to present tumor-associated antigens for the induction of in vivo antitumor immunity. Total murine dermal cells were exposed either to medium alone or to medium containing tumor-associated antigens from S1509a tumor cells. Subsequently, dermal cells were injected subcutaneously at weekly intervals into naïve mice for a total of three immunizations. One week following the final immunization, mice were challenged with living tumor cells. In these experiments, dermal cells pulsed with tumor-associated antigens induced protective immunity to tumor growth. Dermal cells exposed to tumor-associated antigens were also able to elicit delayed-type hypersensitivity after footpad injection into mice previously immunized against S1509a tumor cells. The ability to present tumor-associated antigens for both induction of antitumor immunity and elicitation of delayed-type hypersensitivity was dependent on I-A+ cells and was genetically restricted. Finally, dermal cells tended towards eliciting a greater antitumor delayed-type hypersensitivity response than epidermal cells. These results show that the murine dermis contains antigen-presenting cells capable of processing S1509a tumor antigens for the generation of protective antitumor immunity in vivo.
authors.affiliation: Department of Dermatology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York 10021, USA.
authors.forename: K
authors.initials: K
authors.lastname: Campton
authors.forename: W
authors.initials: W
authors.lastname: Ding
authors.forename: Z
authors.initials: Z
authors.lastname: Yan
authors.forename: H
authors.initials: H
authors.lastname: Ozawa
authors.forename: K
authors.initials: K
authors.lastname: Seiffert
authors.forename: E
authors.initials: E
authors.lastname: Miranda
authors.forename: A
authors.initials: A
authors.lastname: Lonati
authors.forename: S
authors.initials: S
authors.lastname: Beissert
authors.forename: R D
authors.initials: RD
authors.lastname: Granstein
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000938:Antigen-Presenting Cells / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000166:cytology*; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D019496:Cancer Vaccines