Viral activation of interleukin-15 (IL-15): characterization of a virus-inducible element in the IL-15 promoter region.
We identified an interferon regulatory factor motif (IRF-E) upstream of an NF-kappaB binding site in the interleukin-15 (IL-15) promoter. Since these two motifs are part of the virus-inducible enhancer region of the beta interferon promoter, we speculated that there might be similar responses of these two genes to stimuli such as viruses. To test this hypothesis, L929 cells were infected with Newcastle disease virus (NDV), which led to the induction of IL-15 mRNA and protein expression. Using IL-15 promoter-reporter deletion constructs, a virus-inducible region, encompassing IRF-E, NF-kappaB, and a 13-nucleotide sequence flanked by these two motifs, was mapped to the -295-to--243 position relative to the transcription initiation site. Using cotransfection studies, it was demonstrated that all three motifs were essential to achieve the maximum promoter activity induced by IRF-1 and NF-kappaB expression plasmids. The presence of a virus-inducible region in the IL-15 promoter suggests a role for IL-15 as a component of host antiviral defense mechanisms.
Azimi
N
N
Metabolism Branch, Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-1374, USA. nazli@helix.nih.gov
Shiramizu
K M
KM
Tagaya
Y
Y
Mariner
J
J
Waldmann
T A
TA
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D005347:Fibroblasts; D005786:Gene Expression Regulation; D050856:Interferon Regulatory Factor-1; D019409:Interleukin-15 / Q000096:biosynthesis / Q000235:genetics*; D007739:L Cells; D051379:Mice; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D009522:Newcastle disease virus / Q000276:immunology*; D010750:Phosphoproteins / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; D019409:Interleukin-15; C494258:Irf1 protein, mouse; D016328:NF-kappa B; D010750:Phosphoproteins