title: Validation of negligible depletion solid-phase microextraction as a tool to determine tissue/blood partition coefficients for semivolatile and nonvolatile organic chemicals.
abstract: In the current study, negligible depletion solid-phase microextraction (nd-SPME) as a technique to determine tissue/blood (tissue/water and blood/water) partition coefficients was validated. With this method the free fraction of chemicals in water in the presence of different tissues was determined and, subsequently, the partition coefficients were calculated. Liver, blood, muscle, and fat tissues obtained from male Wistar-derived rats (U:Wu) were used without homogenization. Data obtained were compared with literature data for lindane, parathion, and paraoxon. The results show that the data in the present work differ by less than a factor of two from those reported in literature. In addition, the standard deviations obtained show that the technique is accurate. Therefore, we conclude that this accurate and automated method can be used to determine tissue/blood partition coefficients for semivolatile and nonvolatile chemicals.
authors.affiliation: Research Institute of Toxicology, Utrecht University, Utrecht, The Netherlands. Elsa@ritox.vet.uu.nl
authors.forename: E
authors.initials: E
authors.lastname: Artola-Garicano
authors.forename: W H
authors.initials: WH
authors.lastname: Vaes
authors.forename: J L
authors.initials: JL
authors.lastname: Hermens
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005069:Evaluation Studies as Topic; D001556:Hexachlorocyclohexane / Q000032:analysis / Q000493:pharmacokinetics; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008832:Microchemistry / Q000379:methods*; D009132:Muscles / Q000737:chemistry / Q000378:metabolism; D009930:Organic Chemicals / Q000032:analysis* / Q000493:pharmacokinetics; D010261:Paraoxon / Q000032:analysis / Q000493:pharmacokinetics; D010278:Parathion / Q000032:analysis / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012995:Solubility; D014018:Tissue Distribution; D014835:Volatilization; D014867:Water / Q000737:chemistry
chemical_list: D009930:Organic Chemicals; D014867:Water; D001556:Hexachlorocyclohexane; D010278:Parathion; D010261:Paraoxon