Sequence and molecular analysis of the rpoA cluster genes from Xanthomonas campestris pv. campestris.
The Xanthomonas campestris rpsM (S13)-rpsK (S11)-rpsD (S4)-rpoA (alpha)-rplQ (L17) cluster, encoding RNA polymerase alpha-subunit and four ribosomal proteins, reside in a 3164-bp DNA region. The N-terminal sequence of the authentic alpha-protein determined chemically matches that predicted from the nucleotide sequence. rplQ is monocistronic, instead of being co-transcribed with the other genes as in Escherichia coli. Antiserum against the His-tagged alpha-protein cross-reacted with the E. coli alpha-protein.
Lai
J Y
JY
Institute of Molecular Biology, National Chung Hsing University, 402, Taichung, Taiwan.
Huang
C F
CF
Tseng
Y H
YH
Yang
M T
MT
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D016959:Xanthomonas campestris / Q000235:genetics*
D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit