Mtr1, a novel biallelically expressed gene in the center of the mouse distal chromosome 7 imprinting cluster, is a member of the Trp gene family.
We recently described a novel putative Ca(2+) channel gene, MTR1, which shows a high level of homology to the human TRPC7 gene and the melastatin 1 (MLSN1) gene, another Trp (transient receptor potential protein)-related gene whose transcript was found to be downregulated in metastatic melanomas. It maps to human chromosome band 11p15.5, which is associated with the Beckwith-Wiedemann syndrome and predisposition to a variety of neoplasias. Here we report the isolation and characterization of the murine orthologue Mtr1. The chromosomal localization on distal chromosome 7 places it in a cluster of imprinted genes, flanked by the previously described Tapa1 and Kcnq1 genes. The Mtr1 gene encodes a 4.4-kb transcript, present in a variety of fetal and adult tissues. The putative open reading frame consists of 24 exons, encoding 1158 amino acids. Transmembrane prediction algorithms indicate the presence of six membrane-spanning domains in the proposed protein. Imprinting analysis, using RT-PCR on RNA from reciprocal mouse crosses harboring a sequence polymorphism, revealed biallelic expression of Mtr1 transcripts at all stages and tissues examined.
Enklaar
T
T
Children's Hospital, University of Mainz, Langenbeckstrasse 1, Mainz, D-55101, Germany. enklaar@wserv.kinder.klinik.uni-mainz.de
Esswein
M
M
Oswald
M
M
Hilbert
K
K
Winterpacht
A
A
Higgins
M
M
Zabel
B
B
Prawitt
D
D
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015220:Calcium Channels / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D005091:Exons; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005796:Genes / Q000235:genetics; D018392:Genomic Imprinting; D006801:Humans; D007438:Introns; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D050052:TRPC Cation Channels; D050053:TRPM Cation Channels; D014018:Tissue Distribution
D015220:Calcium Channels; D008565:Membrane Proteins; D012333:RNA, Messenger; D050052:TRPC Cation Channels; D050053:TRPM Cation Channels; C404409:TRPM5 protein, human; C498617:Trpm5 protein, mouse; C096351:transient receptor potential cation channel, subfamily C, member 1