Simultaneous determination of alpha-tocopherol and beta-carotene in olive oil by reversed-phase high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method was developed for the determination, in one run, of alpha-tocopherol and beta-carotene in virgin olive oil. The method involved a rapid saponification and a later extraction with a mixture of hexane-ethyl acetate. The chromatographic system consists of an ODS-2 column with a mobile phase of methanol-water-butanol and a diode-array detector. Linearity, precision, recovery and sensitivity were satisfactory. The main advantage of the proposed method is the speed and simultaneous determination of both compounds at the same time.
Gimeno
E
E
Dpt. Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Spain.
Calero
E
E
Castellote
A I
AI
Lamuela-Raventós
R M
RM
de la Torre
M C
MC
López-Sabater
M C
MC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E / Q000032:analysis*; D019207:beta Carotene / Q000032:analysis*
D000069463:Olive Oil; D010938:Plant Oils; D019207:beta Carotene; D014810:Vitamin E