Isolation of rat mitochondrial transcription factor A (r-Tfam) cDNA.
We have isolated rat mitochondrial transcription factor A (Tfam; formerly known as mtTFA) cDNA clones from a rat cerebellum cDNA library using human Tfam cDNA as a probe. The deduced amino acid sequence of r-Tfam shows 62% and 89% overall identity to human and mouse Tfam, respectively. We also show the presence of two r-Tfam isoforms in testis as for mouse. Our findings suggest that the mechanisms underlying transcription of mitochondrial genes are conserved among rat, mouse, and human.
Inagaki
H
H
Department of Chemistry, National Industrial Research Institute of Nagoya, Japan. hinagaki@nirin.go.jp
Hayashi
T
T
Matsushima
Y
Y
Lin
K H
KH
Maeda
S
S
Ichihara
S
S
Kitagawa
Y
Y
Saito
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000378:metabolism; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D020033:Protein Isoforms / Q000235:genetics; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D029867:Xenopus Proteins
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D024101:Mitochondrial Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; C499631:TFAM protein, human; C081930:Tfam protein, mouse; C489030:Tfam protein, rat; D015534:Trans-Activators; D014157:Transcription Factors; C097078:XL-MTTFA protein, Xenopus; D029867:Xenopus Proteins; C422562:mitochondrial transcription factor A