Detection of atrazine based on the SPR determination of P450 mRNA levels in Saccharomyces cerevisiae.
We describe a novel method for quantification of atrazine based on detection of P450 mRNA levels in Saccharomyces cerevisiae. The selected oligonucleotide probe exhibited specificity against P450 mRNA and was successfully immobilized on the sensor chip. The mRNA was subsequently quantified by RU change using a SPR system. When the cells were disrupted by boiling, mRNA could be measured without further purification at reduced sensitivity. This simple technique permits the detection of atrazine within 15 min. This rapid and highly sensitive method can be used for the detection of atrazine.
Lim
T
T
Research Center for Advanced Science and Technology, The University of Tokyo, Japan.
Oyama
M
M
Ikebukuro
K
K
Karube
I
I
D001280:Atrazine / Q000032:analysis*; D002138:Calibration; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D001280:Atrazine