Hatching in zebrafish (Danio rerio) embryos exposed to a 50 Hz magnetic field.
Zebrafish embryos were exposed intra ovo to a 50 Hz AC magnetic field of 1000 microT rms, and the progress of asynchronous hatching was monitored. A statistically significant delay was observed when field exposure started 48 h after fertilization. In contrast, when exposure started 2 h after fertilization, no statistically significant effect was seen. When field exposure was administered together with submaximal doses of progesterone at 48 h postfertilization, the two treatments appeared to delay hatching in an additive manner. Evaluating the progress of hatching in zebrafish embryos seems relevant for exploration of EMF effects on reproduction.
Skauli
K S
KS
Norwegian Radiation Protection Authority, Osterås, Norway. kirsten.sanna.skauli@nrpa.no
Reitan
J B
JB
Walther
B T
BT
D000818:Animals; D005260:Female; D008280:Magnetics / Q000009:adverse effects*; D008297:Male; D011374:Progesterone / Q000494:pharmacology; D012098:Reproduction; D015027:Zebrafish / Q000196:embryology* / Q000254:growth & development / Q000502:physiology
D011374:Progesterone