Transfer of tissue factor from leukocytes to platelets is mediated by CD15 and tissue factor.
We describe thrombogenic tissue factor (TF) on leukocyte-derived microparticles and their incorporation into spontaneous human thrombi. Polymorphonuclear leukocytes and monocytes transfer TF(+) particles to platelets, thereby making them capable of triggering and propagating thrombosis. This phenomenon calls into question the original dogma that vessel wall injury and exposure of TF within the vasculature to blood is sufficient for the occurrence of arterial thrombosis. The transfer of TF(+) leukocyte-derived particles is dependent on the interaction of CD15 and TF with platelets. Both the inhibition of TF transfer to platelets by antagonizing the interaction CD15 with P-selectin and the direct interaction of TF itself suggest a novel therapeutic approach to prevent thrombosis.
Rauch
U
U
Division of Thrombosis Research, Department of Medicine, and The Zena and Michael A. Wiener Cardiovascular Institute, Mount Sinai School of Medicine, New York, NY 10029, USA.
Bonderman
D
D
Bohrmann
B
B
Badimon
J J
JJ
Himber
J
J
Riederer
M A
MA
Nemerson
Y
Y
D001692:Biological Transport; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D002460:Cell Line; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000502:physiology*; D016256:Lewis X Antigen / Q000097:blood*; D016253:Microscopy, Immunoelectron; D013925:Thromboplastin / Q000378:metabolism*; D013927:Thrombosis / Q000097:blood / Q000503:physiopathology
D016256:Lewis X Antigen; D013925:Thromboplastin