title: Cytotoxic effects of 5-fluorocytosine on melanoma cells transduced with cytosine deaminase gene.
abstract: To investigate the cytotoxic effects of 5-fluorocytosine on melanoma cells genetically modified with cytosine deaminase gene, the gene was transduced into the tumor cells with the retroviral method. The cytotoxicity effects of 5-fluorocytosine on the tumor cells were measured with the MTT assay and clonogenic assay. It was found that the prodrug 5-fluorocytosine had significant cytotoxic effects on melanoma cells transduced with cytosine deaminase in vitro. The IC50 value of 5-fluorocytosine on transgenic and nontransgenic melanoma cells was 572 microg/mL(-1) and 3870 microg/mL(-1), respectively. Our experiment demonstrated the potential value of the cytosine deaminase gene/5-fluorocytosine system in the treatment of melanoma.
authors.affiliation: Department of Clinical Pharmacology, Affiliated North Taiping Hospital, Beijing, China.
authors.forename: H
authors.initials: H
authors.lastname: Lü
authors.forename: D
authors.initials: D
authors.lastname: Wu
authors.forename: Y
authors.initials: Y
authors.lastname: Wan
authors.forename: B
authors.initials: B
authors.lastname: Gu
authors.forename: J
authors.initials: J
authors.lastname: Wang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D043525:Cytosine Deaminase; D004354:Drug Screening Assays, Antitumor; D005437:Flucytosine / Q000494:pharmacology*; D008546:Melanoma, Experimental / Q000473:pathology; D051379:Mice; D009700:Nucleoside Deaminases / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000963:Antimetabolites; D005437:Flucytosine; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase