Restriction endonuclease analysis of the genome of two Italian caprine herpesvirus 1 strains.
Two caprine herpesvirus (CpHV.1) strains isolated in two goat flocks in southern Italy were compared with E/CH and McK/US reference CpHV.1 strains by restriction endonuclease analysis. BamHI, KpnI, NotI and PstI restriction enzymes were used. With these enzymes McK/US strain was clearly differentiated from the others. KpnI and NotI digestion resulted in unique patterns for all four strains. Restriction enzyme analysis might be a useful method to distinguish CpHV.1 isolated from animals and provide a basis for further epidemiological studies of the CpHV.1 infection.
Pratelli
A
A
Department of Health and Animal Welfare, University of Bari, Italy.
Greco
G
G
Dall'Ara
P
P
Engels
M
M
Tempesta
M
M
Buonavoglia
C
C
D018138:Alphaherpesvirinae / Q000235:genetics*; D000818:Animals; D016679:Genome, Viral; D006041:Goats; D007558:Italy; D015183:Restriction Mapping