title: Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis.
abstract: A total of 10,154 5'-end expressed sequence tags (EST) were established from the normalized and size-selected cDNA libraries of a marine red alga, Porphyra yezoensis. Among the ESTs, 2140 were unique species, and the remaining 8014 were grouped into 1127 species. Database search of the 3267 non-redundant ESTs by BLAST algorithm showed that the sequences of 1080 species (33.1%) have similarity to those of registered genes from various organisms including higher plants, mammals, yeasts, and cyanobacteria, while 2187 (66.9%) are novel. Codon usage analysis in the coding regions of 101 non-redundant EST groups showing significant similarity to known genes indicated the higher GC contents at the third position of codons (79.4%) than the first (62.2%) and the second position (45.0%), suggesting that the genome has been exposed to high GC pressure during evolution. The sequence data of individual ESTs are available at the web site http://www.kazusa.or.jp/en/plant/porphyra/EST/.
authors.affiliation: Graduate School of Marine Science and Technology, Tokai University, Shimizu, Shizuoka, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Nikaido
authors.forename: E
authors.initials: E
authors.lastname: Asamizu
authors.forename: M
authors.initials: M
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Saga
authors.forename: S
authors.initials: S
authors.lastname: Tabata
mesh_terms: D000465:Algorithms; D003062:Codon; D018076:DNA, Complementary / Q000378:metabolism; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D015723:Gene Library; D008969:Molecular Sequence Data; D000461:Rhodophyta / Q000235:genetics*; D012984:Software
chemical_list: D003062:Codon; D018076:DNA, Complementary