title: Experimental Salmonella typhi infection in the domestic pig, Sus scrofa domestica.
abstract: The domestic pig, Sus scrofa domestica, was examined as a model for typhoid fever, a severe and systemic disease of humans caused by Salmonella typhi. Six pigs were inoculated 1 week post-weaning with approximately 10(10)colony forming units (cfu) of wild type Salmonella typhi strain ISP1820 intranasally and observed for 3 weeks. S. typhi was cultured from the tonsils of 50% of the pigs at necropsy. Cultures from all other organs analysed (ileum, colon, spleen and liver) were negative. No clinical or histopathological signs of disease were observed. Pigs inoculated in parallel with swine-virulent S. choleraesuis all exhibited signs of systemic salmonellosis indicating that the parameters of the experimental infection with S. typhi (e.g. route) were appropriate. Whereas the pig has a gastrointestinal tract that is very similar to humans, our results indicated that the unique features of host and microbe interaction needed to produce typhoid fever were not mimicked in swine. Nevertheless, our observation of tonsillar involvement was consistent with former observations of S. choleraesuis and S. typhimurium infections in swine and supports a role for the tonsil in all porcine salmonella infections.
authors.affiliation: Department of Microbiology and Immunology, F. Edward Hébert School of Medicine, Bethesda, MD 20814-4799, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Metcalf
authors.forename: G W
authors.initials: GW
authors.lastname: Almond
authors.forename: P A
authors.initials: PA
authors.lastname: Routh
authors.forename: J R
authors.initials: JR
authors.lastname: Horton
authors.forename: R C
authors.initials: RC
authors.lastname: Dillman
authors.forename: P E
authors.initials: PE
authors.lastname: Orndorff
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D014066:Palatine Tonsil / Q000382:microbiology; D012475:Salmonella / Q000254:growth & development / Q000472:pathogenicity; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012485:Salmonella typhi / Q000254:growth & development / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014435:Typhoid Fever / Q000382:microbiology*; D014774:Virulence