Effects of collagenase and elastase on the mechanical properties of lung tissue strips.
The dynamic stiffness (H), damping coefficient (G), and harmonic distortion (k(d)) characterizing tissue nonlinearity of lung parenchymal strips from guinea pigs were assessed before and after treatment with elastase or collagenase between 0.1 and 3.74 Hz. After digestion, data were obtained both at the same mean length and at the same mean force of the strip as before digestion. At the same mean length, G and H decreased by approximately 33% after elastase and by approximately 47% after collagenase treatment. At the same mean force, G and H increased by approximately 7% after elastase and by approximately 25% after collagenase treatment. The k(d) increased more after collagenase (40%) than after elastase (20%) treatment. These findings suggest that, after digestion, the fraction of intact fibers decreases, which, at the same mean length, leads to a decrease in moduli. At the same mean force, collagen fibers operate at a higher portion of their stress-strain curve, which results in an increase in moduli. Also, G and H were coupled so that hysteresivity (G/H) did not change after treatments. However, k(d) was decoupled from elasticity and was sensitive to stretching of collagen, which may be of value in detecting structural alterations in the connective tissue of the lung.
Yuan
H
H
Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA.
Kononov
S
S
Cavalcante
F S
FS
Lutchen
K R
KR
Ingenito
E P
EP
Suki
B
B
D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D003094:Collagen / Q000378:metabolism; D017364:Collagenases / Q000494:pharmacology*; D003238:Connective Tissue / Q000187:drug effects / Q000378:metabolism; D004548:Elasticity; D004549:Elastin / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D017711:Nonlinear Dynamics; D010196:Pancreatic Elastase / Q000494:pharmacology*; D013314:Stress, Mechanical
D003094:Collagen; D004549:Elastin; D010196:Pancreatic Elastase; D017364:Collagenases