title: Localization and immunogenicity of a low molecular weight antigen of Eimeria tenella.
abstract: A low molecular weight (LMW) antigen recognized by a murine monoclonal antibody (C(3)4F(1)) was localized within endogenous stages of Eimeria tenella (USDA strain 80). Using indirect fluorescent antibody assay and immunoelectron microscopy, the LMW antigen was found in: sporozoites, first, second and third generation meronts, gamonts, unsporulated oocysts, and sporocysts. The antigen was observed in the cytoplasm and pellicle of the parasite, and in the parasitophorous vacuole, sporocyst walls and cytoplasm of infected host cells. The immunogenicity of this LMW antigen was assessed by antigen-specific serum antibody responses in chickens orally inoculated with live oocysts or injected intramuscularly with dead sporozoites. LMW antigen-specific serum antibodies were detected using Western blots of E. tenella sporozoites as early as 4 days after sporozoite injection and 6 days after oocyst inoculation. Unusually, the monoclonal antibody C(3)4F(1) reduced the binding of immune chicken serum to the antigen in a competitive antibody binding assay, but not the reverse, suggesting that there is a single, immunodominant epitope on this antigen.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Canada.
authors.forename: S A
authors.initials: SA
authors.lastname: Tennyson
authors.forename: J R
authors.initials: JR
authors.lastname: Barta
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification*; D002451:Cell Compartmentation; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology; D016786:Eimeria tenella / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D011201:Poultry Diseases / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan