title: Specialized contributions by alpha(1,3)-fucosyltransferase-IV and FucT-VII during leukocyte rolling in dermal microvessels.
abstract: Noninflamed skin venules support constitutive leukocyte rolling. P-selectin controls the rolling frequency, whereas E-selectin dictates rolling velocity (Vroll). Fucosylated selectin ligands are essential for all interactions, as rolling was absent in mice doubly deficient in alpha1,3-fucosyltransferase (FucT)-IV and FucT-VII. The rolling fraction was reduced in FucT-VII-/- animals but normal in FucT-IV-/- mice. However, Vroll was markedly increased in both strains. P-selectin ligands generated by FucT-VII are crucial for initial leukocyte tethering, whereas E-selectin ligands that permit maximum slowing of Vroll require simultaneous expression of FucT-IV and FucT-VII. These results demonstrate a role for FucT-IV in selectin-dependent adhesion and suggest that the endothelial selectins and FucTs have distinct but overlapping functions in the immunosurveillance of the skin.
authors.affiliation: The Center for Blood Research and Department of Pathology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: W
authors.initials: W
authors.lastname: Weninger
authors.forename: L H
authors.initials: LH
authors.lastname: Ulfman
authors.forename: G
authors.initials: G
authors.lastname: Cheng
authors.forename: N
authors.initials: N
authors.lastname: Souchkova
authors.forename: E J
authors.initials: EJ
authors.lastname: Quackenbush
authors.forename: J B
authors.initials: JB
authors.lastname: Lowe
authors.forename: U H
authors.initials: UH
authors.lastname: von Andrian
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D019040:E-Selectin / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology; D004423:Ear / Q000098:blood supply; D005260:Female; D005647:Fucosyltransferases / Q000378:metabolism / Q000502:physiology*; D007249:Inflammation / Q000201:enzymology / Q000276:immunology; D019041:L-Selectin / Q000235:genetics / Q000502:physiology; D007962:Leukocytes / Q000201:enzymology* / Q000276:immunology* / Q000378:metabolism; D008024:Ligands; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008833:Microcirculation / Q000201:enzymology / Q000276:immunology / Q000378:metabolism; D019007:P-Selectin / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012867:Skin / Q000098:blood supply* / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D014699:Venules / Q000201:enzymology / Q000276:immunology / Q000378:metabolism
chemical_list: D019040:E-Selectin; D008024:Ligands; D019007:P-Selectin; D019041:L-Selectin; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase