Development and validation of a capillary electrophoresis method for direct measurement of isocitric, citric, tartaric and malic acids as adulteration markers in orange juice.
Fruit juices each have very distinct organic acids profiles that can be used as fingerprints for establishing authenticity. A method has been developed, optimised and validated for measuring by capillary electrophoresis citric, isocitric, malic and tartaric acids as authenticity markers in orange juices, without any sample treatment other than dilution and filtration. Final conditions were phosphate buffer 200 mM, pH 7.50, -14 kV as applied potential, and 57 cm length neutral capillary. Detection was direct UV at 200 nm. Different kinds and marks of orange juice, chosen from the great variety existent in the market, were analysed and clear differences could be found between them and just pressed orange juice.
Saavedra
L
L
Facultad de CC Experimentales y Técnicas, Universidad San Pablo-CEU, Urbanización Montepríncipe, Madrid, Spain.
García
A
A
Barbas
C
C
D001628:Beverages / Q000032:analysis*; D019343:Citric Acid / Q000032:analysis*; D002957:Citrus / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007523:Isocitrates / Q000032:analysis*; D008293:Malates / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013644:Tartrates / Q000032:analysis*
D007523:Isocitrates; D008293:Malates; D013644:Tartrates; D019343:Citric Acid; C030298:malic acid; C034219:isocitric acid; C029768:tartaric acid