title: cAMP activates an ATP-permeable pathway in neonatal rat cardiac myocytes.
abstract: The molecular mechanisms associated with intracellular ATP release by the heart are largely unknown. In this study the luciferin-luciferase assay and patch-clamp techniques were used to characterize the pathways responsible for ATP release in neonatal rat cardiac myocytes (NRCM). Spontaneous ATP release by NRCM was significantly increased after cAMP stimulation under physiological conditions. cAMP stimulation also induced an anion-selective electrodiffusional pathway that elicited linear, diphenylamine-2-carboxylate (DPC)-inhibitable Cl(-) currents in either symmetrical MgCl(2) or NaCl. ATP, adenosine 5'-O-(3-thiotriphosphate), and the ATP derivatives ADP and AMP, permeated this pathway; however, GTP did not. The cAMP-induced ATP currents were inhibited by DPC and glibenclamide and by a monoclonal antibody raised against the R domain of the cystic fibrosis transmembrane conductance regulator (CFTR). The channel-like nature of the cAMP-induced ATP-permeable pathway was also determined by assessing protein kinase A-activated single channel Cl(-) and ATP currents in excised inside-out patches of NRCM. Single channel currents were inhibited by DPC and the anti-CFTR R domain antibody. Thus the data in this report demonstrate the presence of a cAMP-inducible electrodiffusional ATP transport mechanism in NRCM. Based on the pharmacology, patch-clamping data, and luminometry studies, the data are most consistent with the role of a functional CFTR as the anion channel implicated in cAMP-activated ATP transport in NRCM.
authors.affiliation: Renal Unit, Massachusetts General Hospital East, Charlestown 02129, Massachusetts, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Lader
authors.forename: Y F
authors.initials: YF
authors.lastname: Xiao
authors.forename: C R
authors.initials: CR
authors.lastname: O'Riordan
authors.forename: A G
authors.initials: AG
authors.lastname: Prat
authors.forename: G R
authors.initials: GR
authors.lastname: Jackson
authors.forename: H F
authors.initials: HF
authors.lastname: Cantiello
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D000906:Antibodies / Q000494:pharmacology; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D018118:Chloride Channels / Q000502:physiology; D002712:Chlorides / Q000502:physiology; D000242:Cyclic AMP / Q000494:pharmacology / Q000502:physiology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000276:immunology / Q000502:physiology; D004553:Electric Conductivity; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats
chemical_list: D000906:Antibodies; D018118:Chloride Channels; D002712:Chlorides; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000255:Adenosine Triphosphate; D000242:Cyclic AMP