Plasma concentrations of blood coagulation factor VII measured by immunochemical and amidolytic methods.
Ever since the coagulant activity of blood coagulation factor VII (FVII:C) was identified as a risk indicator of cardiac death, a large number of studies have measured FVII protein concentrations in plasma. FVII protein concentrations are either measured immunologically with an ELISA method (FVII:Ag) or estimated with an amidolytic method (FVII:Am). We have investigated whether FVII:Am is a valuable alternative to FVII:Ag. FVII:Ag and FVII:Am were measured in 147 plasma samples from blood donors, patients on oral anticoagulant therapy, postmenopausal women on hormone replacement therapy, in postprandial plasma, in cold activated plasma and in FVII deficient plasma. There was a positive correlation (r=0.96) between FVII:Ag and FVII:Am with slightly but significantly higher values for FVII:Ag (FVII:Ag= 106 U/ml and FVII:Am=100 U/ml; p <0.001). A significant correlation (r=0.93) was still observed after omitting the data from patients on oral anticoagulant therapy, with mean values of 113 U/ml for FVII:Ag and 110 U/ml for FVII:Am (p < 0.01). In a linear regression analysis, the intercept (alpha=-21.50) was different from zero (p < 0.0001) and the slope (beta=1.16) was different from 1.0 (p < 0.001). Only a few points (3.1%) in a difference plot were present outside the expected 95% interval calculated from the analytical imprecision of the two methods. Therefore, we consider the amidolytic method an acceptable alternative to the ELISA method when analysing citrated plasma samples containing only enzymatically functional FVII protein, but the results cannot be directly transferred from one method to the other.
Bladbjerg
E M
EM
Department of Thrombosis Research, University of Southern Denmark, Esbjerg. emb@ribeamt.dk
Gram
J
J
Jespersen
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants / Q000627:therapeutic use; D004041:Dietary Fats / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D015914:Estrogen Replacement Therapy; D005167:Factor VII / Q000032:analysis*; D005168:Factor VII Deficiency / Q000097:blood / Q000175:diagnosis; D015942:Factor VIIa / Q000032:analysis*; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged
D000925:Anticoagulants; D004041:Dietary Fats; D005167:Factor VII; D015942:Factor VIIa