Capillary zone electrophoresis for the determination of atovaquone in serum.
A rapid and simple capillary zone electrophoresis (CZE) method has been developed for the determination of atovaquone in serum. The drug was extracted from equine serum-chloroform (1:3, v/v) at greater than 80% recovery and assayed in buffer containing 25 mM sodium borate (pH 9.1) and 25% acetonitrile. A 100 microm I.D. fused-silica capillary was used and the detection was by UV-diode array at 254 nm; the migration time was approximately 8 min. Intra- and inter-assay variabilities were less than 7.8% and 5.8%, respectively, and the accuracy of the assay (expressed as % bias) ranged from 4.5 to -5.2%. The working assay range was from 2 to 100 microg/ml. This sensitivity could be increased by concentrating during the extraction procedure. Replacement of acetonitrile with 75 mM surfactant 3-(dimethyldodecylammonio)propanesulfonate gave similar sensitivity and provided an additional option to facilitate the separation of atovaquone on multiple-drug samples.
Chou
C C
CC
Department of Large Animal and Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA. chouc@mail.vetmed.ufl.edu
Brown
M P
MP
Merritt
K A
KA
D000935:Antifungal Agents / Q000097:blood*; D053626:Atovaquone; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009285:Naphthoquinones / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D009285:Naphthoquinones; D053626:Atovaquone