Nitric oxide inhibits the basic fibroblast growth factor-stimulated migration of bovine vascular smooth muscle cells in vitro.
BACKGROUND
The purpose of this study was to evaluate the effect of nitric oxide (NO) on basic fibroblast growth factor (bFGF)-stimulated migration of bovine VSMC in vitro.


METHODS
For the migration assay 50,000 cells from passages 4 to 8 were put into a migration chamber and stimulated with 10 ng bFGF/ml (control group). For another experiment, the NO donator sodium nitroprusside (SNP) (10(-6), 10(-8), 10(-10) M), the second messenger of NO 8-bromo-cGMP (10(-6), 10(-8), 10(-10) M) and 100 U of superoxide dismutase (SOD) were added. Migration of cells was quantified after fixing and staining of the cells by means of light microscopy.


RESULTS
Cell migration was significantly inhibited by SNP in a dose dependent manner (p < 0.01 with t-test). This effect was mirrored by 8-bromo-cGMP and not significantly inhibited by SOD, confirming this to be an NO mediated effect and excluding side-effects of superoxide-derived oxidation products.


CONCLUSIONS
Nitric oxide inhibits the basic fibroblast growth factor stimulated migration of bovine vascular smooth muscle cells in vitro.
Alfke
H
H
Department of Radiology, Philipps University of Marburg, Germany. alfke@mailer.uni-marburg.de
Kleb
B
B
Klose
K J
KJ
D000818:Animals; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D009569:Nitric Oxide / Q000494:pharmacology*
D016222:Fibroblast Growth Factor 2; D009569:Nitric Oxide