title: Mitochondrial DNA sequences of greenbug (Homoptera: Aphididae) biotypes.
abstract: Sequence comparisons were made for 738-bp of mtDNA cloned from seven greenbug, Schizaphis graminum, biotypes (B, C, E, F, G, H and I) obtained from laboratory colonies maintained by USDA-ARS, Stillwater, OK. These sequences include parts of the genes for 16S ribosomal subunit (16S rRNA), tRNAleu, tRNAser, cytochrome b (cytb) and NADH dehydrogenase (ND) subunits one and four. Sequence data revealed considerable variation in 86 (12%) nucleotide sites over the 738-bp sequenced among the seven greenbug biotypes. Nucleotide invariance was observed within the seven greenbug biotypes from both the laboratory colonies and field collected biotype E greenbugs from Kansas, Nebraska, Oklahoma, and Texas.
authors.affiliation: Department of Entomology, University of Nebraska-Lincoln, 68583, USA. bondlab@msm.edu
authors.forename: F O
authors.initials: FO
authors.lastname: Aikhionbare
authors.forename: Z B
authors.initials: ZB
authors.lastname: Mayo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003573:Cytochrome b Group / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial; D016054:DNA, Protozoan; D012336:RNA, Ribosomal, 16S; D012343:RNA, Transfer; D009245:NADH Dehydrogenase