title: Identification of microsatellite length polymorphism by a polymerase chain reaction-based assay at IL-1beta gene locus in cancer cells.
abstract: It has long been considered that genetic instability is an integral component of human neoplasia, however the frequency of microsatellite instability in human melanoma and non-melanoma skin cancer was less evidenced. We report here an alteration at the complex dinucleotide repeat (TC)n sequence in the interleukin 1-beta gene locus by polymerase chain reaction (PCR)-based assay in mouse melanoma as well as in various cancer cells, which may provide a simple, rapid, non-radioactive screening assay for cancer detection.
authors.affiliation: Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520, USA. ashok.chakraborty@yale.edu
authors.forename: A K
authors.initials: AK
authors.lastname: Chakraborty
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000235:genetics; D008546:Melanoma, Experimental / Q000235:genetics*; D051379:Mice; D008811:Mice, Inbred DBA; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D007375:Interleukin-1; D004247:DNA