title: [Rapid progression of passaged mouse hepatocarcinoma associated with the loss of cell polarity].
abstract: Two transplantable differentiated mouse hepatocarcinomas were obtained in B6 D2 F1 mice from primary tumors induced by initiation (NDEA)-promotion (phenobarbital) protocol. Both HC were slowly growing with time from passage to passage of 5-7 months (s-HC). A fast-growing variant with time between passages of 2-3 weeks appeared in one mouse on the third passage (f-HC). The three HC strains constantly retained their phenotype. The s-HCs were characterized by prominent cell polarity according to organization of the cytoskeleton and distribution of domain-specific membrane-associated markers. Cell polarity was completely destroyed in the fast-growing variant. Cell adhesion in the latter tumor was very low. An in vitro growing strain of f-HC was easily obtained enabling experimental study of regulation of progression in HCs.
authors.affiliation: N. N. Blokhin Russian Cancer Research Center, Moscow.
authors.forename: N V
authors.initials: NV
authors.lastname: Engel'gardt
authors.forename: E I
authors.initials: EI
authors.lastname: Kudriavtseva
authors.forename: O V
authors.initials: OV
authors.lastname: Morozova
authors.forename: T D
authors.initials: TD
authors.lastname: Rudinskaia
authors.forename: V S
authors.initials: VS
authors.lastname: Turusov
mesh_terms: D000818:Animals; D002455:Cell Division; D016764:Cell Polarity; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D051379:Mice; D014407:Tumor Cells, Cultured