title: Inhibition of azoxymethane-induced colon cancer by orange juice.
abstract: Previous research has shown that hesperidin, a flavanone glycoside in orange juice, inhibits colon carcinogenesis and that feeding double-strength orange juice delays the onset of chemically induced mammary cancer in rats. This study determined whether feeding single-strength, pasteurized orange juice would inhibit azoxymethane (AOM)-induced colon cancer in male Fischer 344 rats. Colon cancer was initiated by injecting AOM (15 mg/kg body wt) at 22 and 29 days of age. One week after the second AOM injection, orange juice replaced drinking water for the experimental group (n = 30). The rats were killed 28 weeks later, and tumors were removed for histological analysis. Feeding orange juice reduced tumor incidence by 22% (p < 0.05). Tumor reduction was associated with a decreased labeling index and proliferation zone in the colonic mucosa. Hesperidin, other flavonoids, limonin 17-beta-D-glucopyranoside, and other limonoid glucosides are potential chemopreventive agents in orange juice that could account for the decreased colon tumorigenesis associated with feeding orange juice.
authors.affiliation: Michigan State University, East Lansing 48824-1224, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Miyagi
authors.forename: A S
authors.initials: AS
authors.lastname: Om
authors.forename: K M
authors.initials: KM
authors.lastname: Chee
authors.forename: M R
authors.initials: MR
authors.lastname: Bennink
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001397:Azoxymethane; D001628:Beverages; D002273:Carcinogens; D002957:Citrus / Q000737:chemistry / Q000627:therapeutic use*; D003106:Colon / Q000503:physiopathology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D008517:Phytotherapy; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D000970:Antineoplastic Agents; D002273:Carcinogens; D001397:Azoxymethane