title: Growth of Candida boidinii on methanol and the activity of methanol-degrading enzymes as affected from formaldehyde and methylformate.
abstract: Formaldehyde and methylformate affect the growth of Candida boidinii on methanol and the activity of methanol-degrading enzymes. The presence of both intermediates in the feeding medium caused an increase in biomass yield and productivity and a decrease in the specific rate of methanol consumption. In the presence of formaldehyde, the activity of formaldehyde dehydrogenase and formate dehydrogenase was essentially increased, whereas the activity of methanol oxidase was decreased. On the contrary, the presence of methylformate caused an increase of the activity of methanol oxidase and a decrease of the activity of formaldehyde dehydrogenase and formate dehydrogenase. Interpretations concerning the yeast behavior in the presence of intermediate oxidation products were considered and discussed.
authors.affiliation: Department of Agricultural Biotechnology, Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, 75 Iera Odos, GR-118 55, Athens, Greece. gaggel@aua.gr
authors.forename: G
authors.initials: G
authors.lastname: Aggelis
authors.forename: N
authors.initials: N
authors.lastname: Margariti
authors.forename: C
authors.initials: C
authors.lastname: Kralli
authors.forename: F
authors.initials: F
authors.lastname: Flouri
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D002175:Candida / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000378:metabolism; D005563:Formic Acid Esters / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D005563:Formic Acid Esters; D005557:Formaldehyde; C025468:methyl formate; D000429:Alcohol Oxidoreductases; C029293:methanol oxidase; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D000432:Methanol