title: Functional significance of prop-1 gene expression in pituitary adenomas.
abstract: Prophet of Pit-1 (Prop-1), which is a paired-like homeodomain transcription factor, is capable of binding to sites in an early enhancer of the Pit-1 gene and regulating its expression. As human Pit-1 is expressed considerably in pituitary adenomas, we studied human Prop-1 gene expression in pituitary adenomas. We also sequenced the Prop-1 cDNAs in pituitary adenomas. Human Prop-1 transcript was detected in all pituitary adenomas examined by RT-PCR analysis. The expression of human Prop-1 in pituitary adenomas was confirmed by in situ hybridization in one of the GH-producing adenomas. The sequence analysis of human Prop-1 cDNAs in these pituitary adenomas revealed that there were no mutations except 5 silent nucleic acid substitutions, suggesting that mutations of Prop-1 gene do not represent a frequent mechanism of human pituitary tumorigenesis.
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Usui
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Mizuta
authors.forename: H
authors.initials: H
authors.lastname: Murabe
authors.forename: S
authors.initials: S
authors.lastname: Muro
authors.forename: M
authors.initials: M
authors.lastname: Suda
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: I
authors.initials: I
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Shimatsu
authors.forename: K
authors.initials: K
authors.lastname: Nakao
mesh_terms: D000236:Adenoma / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D001483:Base Sequence / Q000235:genetics; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D010911:Pituitary Neoplasms / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018398:Homeodomain Proteins; C102134:Prophet of Pit-1 protein