title: Avoidance of solidification of sesame oil at low temperature by self-interesterification with immobilized lipase.
abstract: To reduce the freezing point of sesame oil, the lipase-catalyzed interesterification of sesame oil in a solvent free system was studied. The lipase was immobilized on Celite and refined sesame oil was used as the only substrate for the reaction. After interesterification, the oil did not solidify at 0 degrees C even after 24 h, and even longer storage at 2-4 degrees C did not result in solidification. The change of physical behavior was investigated with a differential scanning calorimeter and X-ray diffraction, and reduction in the thermodynamic and crystallographic stability of the interesterified oil was demonstrated. The change in triacylglycerol species composition after the reaction was analyzed, showing that content of trisaturated acylglycerol was decreased.
authors.affiliation: Fermentation and Food Experiment Station, Kagawa Prefectural Government, Syozu, Japan. hakkou@mb.kagawa-net.or.jp
authors.forename: H
authors.initials: H
authors.lastname: Shibasaki
authors.forename: T
authors.initials: T
authors.lastname: Yamane
mesh_terms: D002152:Calorimetry, Differential Scanning; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000032:analysis; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005615:Freezing; D005989:Glycerides / Q000032:analysis; D008049:Lipase / Q000378:metabolism*; D012715:Sesame Oil / Q000737:chemistry* / Q000378:metabolism; D014280:Triglycerides / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D004075:Diglycerides; D004800:Enzymes, Immobilized; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D014280:Triglycerides; D012715:Sesame Oil; D008049:Lipase