The structure of the carbohydrate backbone of the lipopolysaccharides from Bordetella hinzii and Bordetella bronchiseptica.
The structure of the core-lipid A region of the lipopolysaccharides from Bordetella hinzii and Bordetella bronchiseptica has been analyzed. Lipopolysaccharides were deacylated using strong alkaline hydrolysis, the products were separated by high performance anion-exchange chromatography and analyzed by NMR and mass spectrometry. The following structure of the products can be deduced from the experimental results: where for the product from Bordetella hinzii N = H, R = H, beta-FucN4N- or partially N-acetylated Sug-(1-3)-beta-FucN4N and for the product from Bordetella bronchiseptica N = alpha-Hep, R = H, beta-FucN4N, beta-FucN4NMe or partially N-acetylated Sug-(1-3)-beta-FucN4N or Sug-(1-3)-beta-FucN4NMe; Sug = 2,3-diamino-2,3, 4-trideoxy-hex-4-enuronopyranosyl.
Vinogradov
E
E
Institute for Biological Sciences, National Research Council, Ottawa, Canada. evguenii.vinogradov@nrc.ca
D001884:Bordetella / Q000737:chemistry*; D016950:Bordetella bronchiseptica / Q000737:chemistry*; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry
D008070:Lipopolysaccharides; D009844:Oligosaccharides