title: Cloning and molecular characterization of hrpX from Xanthomonas axonopodis pv. citri.
abstract: The hrpX gene of plant pathogenic Xanthomonas species is essential for pathogenicity on host plants and to cause hypersensitive reaction on non-host plants. We cloned and analyzed a hrpX homologue, designated hrpXct, of X. axonopodis pv. citri, a pathogen of citrus canker. The open reading frame of hrpXct has 1431 bp in nucleotides which has a coding capacity of 476 amino acid residues with a molecular mass of 52.4 kDa. The predicted amino acid sequence of HrpXct has 90% identity to the AraC family type transcriptional activator protein HrpXc of X. campestris pv. campestris, 95% to HrpXo of X. oryzae pv. oryzae and 97% to X. vesicatoria. These findings clearly indicate and confirm that the structure of the hrpX genes in plant pathogenic Xanthomonas species is highly conserved.
authors.affiliation: School of Bioresources, Hiroshima Prefectural University, Shoubara-city, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iwamoto
authors.forename: T
authors.initials: T
authors.lastname: Oku
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014974:Xanthomonas / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C080371:HrpX protein, Bacteria; D014157:Transcription Factors