title: Human immunodeficiency virus-associated malignant lymphoma in eastern Denmark diagnosed from 1990-1996: clinical features, histopathology, and association with Epstein-Barr virus and human herpesvirus-8.
abstract: The clinicopathological features of human immunodeficiency virus (HIV)-associated lymphoma were investigated in a retrospective study of 85 adult patients in eastern Denmark diagnosed during the period 1990-1996. The possible pathogenetic role of Epstein-Barr virus (EBV) and human herpesvirus 8 (HHV-8) in these tumours was also studied. Seventy patients (82%) presented with extranodal disease and 26 (31%) had CNS involvement at diagnosis. Diffuse large cell B-cell lymphoma was the most frequent histological subtype, comprising 65 of 79 cases available for microscopic re-evaluation (82%) and including 20 of 23 evaluable patients with CNS lymphoma (87%). EBV RNA was demonstrated by in situ hybridization in 51 of 65 evaluable tumours (79%) and in 14 of 16 cases (88%) with CNS-lymphoma. Three cases showed a T-cell phenotype. The presence of HHV-8 DNA was analysed by PCR in 32 cases. A strong band consistent with tumour cell infection was detected in only one case, weaker bands being seen in 4 cases. None of these patients had primary effusion lymphomas. In conclusion, Danish AIDS-related lymphomas are of predominantly high-grade B-cell type with extranodal localization and atypical presentation. Our results provide further evidence that EBV plays a major role in the pathogenesis of large cell AIDS-related lymphoma, whereas HHV-8 does not appear to contribute significantly to the development of solid lymphomas in this group of patients.
authors.affiliation: Department of Infectious Diseases, University of Copenhagen, Rigshospitalet, Denmark.
authors.forename: P B
authors.initials: PB
authors.lastname: Hansen
authors.forename: M
authors.initials: M
authors.lastname: Penkowa
authors.forename: O
authors.initials: O
authors.lastname: Kirk
authors.forename: P
authors.initials: P
authors.lastname: Skinhøj
authors.forename: C
authors.initials: C
authors.lastname: Pedersen
authors.forename: I
authors.initials: I
authors.lastname: Lisse
authors.forename: K
authors.initials: K
authors.lastname: Kiss
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: S J
authors.initials: SJ
authors.lastname: Hamilton-Dutoit
mesh_terms: D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D003520:Cyclophosphamide / Q000008:administration & dosage; D004279:DNA, Viral / Q000032:analysis; D003718:Denmark; D004317:Doxorubicin / Q000008:administration & dosage; D004854:Herpesvirus 4, Human / Q000235:genetics; D019288:Herpesvirus 8, Human / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D016483:Lymphoma, AIDS-Related / Q000188:drug therapy / Q000473:pathology* / Q000821:virology*; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000821:virology; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D011241:Prednisone / Q000008:administration & dosage; D012367:RNA, Viral / Q000032:analysis; D012189:Retrospective Studies; D016896:Treatment Outcome; D014750:Vincristine / Q000008:administration & dosage
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D014750:Vincristine; D004317:Doxorubicin; D003520:Cyclophosphamide; D011241:Prednisone