title: [Cathepsin L-like activity in hemolymph of females crammed with Boophilus microplus].
abstract: The cysteine-proteinase activity present in the hemolymph of females crammed with Boophilus microplus at the second oviposition was proved. The enzyme assay was performed using the synthetic N-carbobenzoxi-phenilalanyl-arginil-4-metoxibetan++  +-naphthylamide substrate, and hydrolysis in both raw ovarim extract and hemolymph were observed. The activity was 100% inhibited by trans-epoxisuscinyl-L-leucidamide (4-guadinine) butane, an specific inhibitor for cysteine-proteinase. Polyacrylamide and gelatin-gel electrophoresis showed the existence of only one active band in both samples tested with the same electrophoretic sliding (approximately 38 kDa).
authors.affiliation: ayme@ipk.sld.cu
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Calienes Valdés
authors.forename: J
authors.initials: J
authors.lastname: Mendiola Martínez
authors.forename: H
authors.initials: H
authors.lastname: Hernández Alvarez
authors.forename: C M
authors.initials: CM
authors.lastname: Villalvilla
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000097:blood*; D005260:Female; D006458:Hemolymph; D010271:Parasites
chemical_list: D002403:Cathepsins