title: Detection of Chlamydia trachomatis DNA in archival paraffinized specimens from chronic salpingitis cases using the polymerase chain reaction.
abstract: OBJECTIVE
  To identify Chlamydia trachomatis by the polymerase chain reaction (PCR) in fallopian tube tissues with chronic salpingitis.
  
  
  METHODS
  Retrospective case-control study.
  
  
  METHODS
  Academic tertiary institution.
  
  
  METHODS
  Women with a pathological diagnosis of chronic salpingitis or normal fallopian tube hospitalized between September 1992 and November 1994. Initial identification of 248 specimens with final analysis of 154.
  
  
  METHODS
  Paraffin-embedded fallopian tube tissues were analyzed with use of PCR to detect C. trachomatis.
  
  
  METHODS
  Identification of C. trachomatis DNA; demographics of age, ethnicity, parity, history of sexually transmitted disease, and surgical procedure.
  
  
  RESULTS
  C. trachomatis DNA was detected in 9 of 77 chronic salpingitis cases. Seventy-seven controls were negative for C. trachomatis. No statistically significant difference in age or ethnicity between cases and controls was identified. Nulliparity was more frequent in cases (26 of 74) than controls (14 of 76). Sexually transmitted disease history was more prevalent in cases (24 of 74) than controls (6 of 76). Chlamydia infection was not associated with a particular surgical indication.
  
  
  CONCLUSIONS
  Chronic salpingitis is highly associated with the presence of C. trachomatis infection as detected by PCR.
authors.affiliation: Greater Baltimore Medical Center, Maryland, USA. ehinton@earthlink.net
authors.forename: E L
authors.initials: EL
authors.lastname: Hinton
authors.forename: L D
authors.initials: LD
authors.lastname: Bobo
authors.forename: T C
authors.initials: TC
authors.lastname: Wu
authors.forename: R J
authors.initials: RJ
authors.lastname: Kurman
authors.forename: R P
authors.initials: RP
authors.lastname: Viscidi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002690:Chlamydia Infections / Q000150:complications / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics*; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D010232:Paraffin; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D012488:Salpingitis / Q000209:etiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D010232:Paraffin