Glucosinolates in members of the family brassicaceae: separation and identification by LC/ESI-MS-MS.
Seeds of 14 different members of the family Brassicaceae were investigated with regard to their content and composition of glucosinolates by HPLC-UV/ESI-MS-MS coupling. The seeds were extracted with hot methanol/water (70:30 v/v) and the desulfoglucosinolates isolated by anion-exchange chromatography with solid-phase extraction columns. The desulfoglucosinolates were detected by UV and identified by ESI-MS/MS with the neutral loss method. Nineteen different glucosinolates were detected in the seeds with a wide range of contents (10-200 micromol/g) and a great variation in the composition.
Matthäus
B
B
Institut für Chemie und Physik der Fette der Bundesanstalt für Getreide-, Kartoffel- und Fettforschung, Münster, Germany. matthaus@uni-muenster.de
Luftmann
H
H
D001937:Brassica / Q000737:chemistry*; D019607:Brassicaceae / Q000737:chemistry*; D005961:Glucosinolates / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods
D005961:Glucosinolates