title: Expression of chemokine receptors on CD4+ T cells in peripheral blood from HIV-infected individuals in Uganda.
abstract: CXCR4, a coreceptor for T cell (T)-tropic HIV-1, is preferentially expressed on naive T cells, whereas CCR5, a coreceptor for macrophage (M)-tropic HIV-1, is preferentially expressed on previously activated memory T cells and the Th1 subset of CD4+ T cells. CCR4 is preferentially expressed on the Th2 subset of CD4+ T cells. A cross-sectional flow cytometry study was conducted to evaluate the expression of CXCR4, CCR5, and CCR4 on the peripheral blood CD4+ T cells from African HIV-1-infected and uninfected Ugandan adults. The plasma viral load in HIV-1-infected individuals was also examined. Upregulation of CCR4 and CCR5 expression but no decrease in CXCR4 expression on CD4+ T cells were obtained in peripheral blood from African adults with progression of the disease. Plasma HIV-1 viremia significantly and inversely correlated with the peripheral CD4+ T cell count but did not correlate with the degree of CCR4 and CCR5 expression on the peripheral CD4+ T cells in HIV-1-infected individuals. Our present data suggest an increase in percentage of activated memory CD4+ T cells in the advanced stage of HIV-1 infection among African adults. There was no evidence of a Th1 to Th2 shift in terms of chemokine receptor expression profile with advancing disease in the peripheral blood of these subjects.
authors.affiliation: Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Japan. oishi-k@net.nagasaki-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Oishi
authors.forename: M
authors.initials: M
authors.lastname: Hayano
authors.forename: H
authors.initials: H
authors.lastname: Yoshimine
authors.forename: S B
authors.initials: SB
authors.lastname: Tugume
authors.forename: A
authors.initials: A
authors.lastname: Kebba
authors.forename: R
authors.initials: R
authors.lastname: Mugerwa
authors.forename: P
authors.initials: P
authors.lastname: Mugyenyi
authors.forename: A
authors.initials: A
authors.lastname: Kumatori
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
authors.forename: T
authors.initials: T
authors.lastname: Nagatake
mesh_terms: D000328:Adult; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D006801:Humans; D008297:Male; D054398:Receptors, CCR4; D019713:Receptors, CCR5 / Q000097:blood; D019718:Receptors, CXCR4 / Q000097:blood; D019707:Receptors, Chemokine / Q000097:blood*; D014454:Uganda; D014766:Viremia / Q000097:blood / Q000276:immunology
chemical_list: C515354:CCR4 protein, human; D054398:Receptors, CCR4; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D019707:Receptors, Chemokine