Phylogenetic studies of tospoviruses (family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs.
Analysis of the intergenic region (IGR) of S and M RNAs of tospoviruses (Family Bunyaviridae) indicated their heterogeneity both in length and sequence. In general, IGRs of M RNA were shorter in length compared to the IGRs of their respective S RNA species. Percent identity among the S RNA IGR sequences of distinct tospovirus species varied from 42 to 57%, whereas it was 79 to 99% among isolates of the same species. Similarly, when IGRs of M RNAs were compared, there was higher sequence identity among isolates of the same tospovirus species (84 to 98%) than among distinct tospovirus species (46 to 59%). Percent nucleotide identities and maximum likelihood trees of IGR sequences of S and M RNAs indicated that their sequence divergence is similar to that of nucleocapsid gene at inter and intra-species levels. This is the first detailed sequence analysis of IGRs of S and M RNAs of known tospoviruses.
Pappu
S S
SS
Department of Entomology, University of Georgia, Tifton, USA.
Bhat
A I
AI
Pappu
H R
HR
Deom
C M
CM
Culbreath
A K
AK
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D016679:Genome, Viral; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D013045:Species Specificity; D017865:Tospovirus / Q000145:classification* / Q000235:genetics*
D017931:DNA Primers; D012367:RNA, Viral