Spatially addressed synthesis of amino- and amino-oxy-substituted 1, 3,5-triazine arrays on polymeric membranes.
Effective spatially addressed parallel assembly of trisamino- and amino-oxy-1,3,5-triazines was achieved by applying the SPOT-synthesis technique on cellulose and polypropylene membranes. In addition to developing a suitable linker strategy and employing amines and phenolate ions as building blocks, a highly effective microwave-assisted nucleophilic substitution procedure at membrane-bound monochlorotriazines was developed. The 1,3, 5-triazines obtained could be cleaved in parallel from the solid support by TFA vapor to give compounds adsorbed on the membrane surface in a conserved spatially addressed format for analysis and screening. The reaction conditions developed were employed for the synthesis of 8000 cellulose-bound 1,3,5-triazines which were probed in parallel for binding to the anti-transforming growth factor-alpha monoclonal antibody Tab2 in order to identify epitope mimics.
Scharn
D
D
Institut für Medizinische Immunologie, Universitätsklinikum Charité, Humboldt-Universität zu Berlin, Schumannstr. 20-21, D-10098 Berlin, Germany.
Wenschuh
H
H
Reineke
U
U
Schneider-Mergener
J
J
Germeroth
L
L
D000588:Amines; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015195:Drug Design; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010087:Oxides; D010455:Peptides / Q000737:chemistry; D011108:Polymers; D013329:Structure-Activity Relationship; D016211:Transforming Growth Factor alpha / Q000737:chemistry / Q000276:immunology*; D014227:Triazines / Q000138:chemical synthesis* / Q000737:chemistry
D000588:Amines; D000911:Antibodies, Monoclonal; D000939:Epitopes; D008567:Membranes, Artificial; D010087:Oxides; D010455:Peptides; D011108:Polymers; D016211:Transforming Growth Factor alpha; D014227:Triazines