title: Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography.
abstract: A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).
authors.affiliation: Dpt. Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gimeno
authors.forename: A I
authors.initials: AI
authors.lastname: Castellote
authors.forename: R M
authors.initials: RM
authors.lastname: Lamuela-Raventós
authors.forename: M C
authors.initials: MC
authors.lastname: de la Torre
authors.forename: M C
authors.initials: MC
authors.lastname: López-Sabater
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E / Q000032:analysis*
chemical_list: D010938:Plant Oils; D014810:Vitamin E