Short communication: salt extends the upper temperature limit for growth of Lactococcus lactis ssp. cremoris on solid M17 medium.
We have determined conditions for plating of the Lactococcus lactis ssp. cremoris laboratory strain MG1363 on solid M17 broth at 38 degrees C, which is required for the optimal use of the pGhost plasmids. The addition of 1% NaCl (or KCl, potassium acetate, or sucrose at 170 mM) to M17 agar plates results in extension of the upper temperature limit for growth from 37 to 40 degrees C; no decrease in plating efficiency was detected from 30 to 39 degrees C.
Kilstrup
M
M
Department of Microbiology, Technical University of Denmark, Lyngby. immk@pop.dtu.dk
Hammer
K
K
D003470:Culture Media; D013294:Lactococcus lactis / Q000254:growth & development*; D011189:Potassium Chloride / Q000494:pharmacology; D019346:Sodium Acetate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology*; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
D003470:Culture Media; D012965:Sodium Chloride; D019346:Sodium Acetate; D013395:Sucrose; D011189:Potassium Chloride