Regulation of interleukin 12 and interleukin 10 expression in vaccinia virus-infected human monocytes and U-937 cell line.
Regulation of IL-12 and IL-10 production in normal human monocytes infected with vaccinia virus (VV) was analysed. IL-12 and IL-10 mRNAs were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and IL-12 and IL-10 protein by ELISA. RT-PCR analysis revealed a marked-up regulation of IL-12 (p40) and IL-10 expression in virally infected cells compared with that from control (non-infected) cells at 24 h post-infection (p.i.). IL-12 transcripts occurred earlier (at 4 h p.i.) than IL-10 mRNA. A significant increase in IL-12 and IL-10 secretion into the medium was caused by the virus, and even a much more pronounced increase in both interleukins expression (mRNAs and proteins) followed LPS or Staphylococcus aureus treatment. Vaccinia virus infection did not alter IL-10 secretion and IL-10 mRNA content (or even cause a decrease) in a human monocytic cell line U937. Undetectable levels of IL-12 protein were found in the cell line although the transcripts were present in the cells at first hours p.i. It appears now that vaccinia virus transiently and sequentially induces IL-12 and IL-10 in human monocytes.
Slezak
K
K
Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Guzik
K
K
Rokita
H
H
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics*; D018664:Interleukin-12 / Q000096:biosynthesis / Q000235:genetics*; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000821:virology*; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger; D018808:Transcription Factor AP-1 / Q000378:metabolism; D020298:U937 Cells; D014616:Vaccinia virus / Q000276:immunology* / Q000502:physiology; D014709:Vero Cells
D016328:NF-kappa B; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D016753:Interleukin-10; D018664:Interleukin-12