Free radical scavenging and antioxidant effects of lactate ion: an in vitro study.
Divergent literature data are found concerning the effect of lactate on free radical production during exercise. To clarify this point, we tested the pro- or antioxidant effect of lactate ion in vitro at different concentrations using three methods: 1) electron paramagnetic resonance (EPR) was used to study the scavenging ability of lactate toward the superoxide aion (O(2)(-).) and hydroxyl radical (.OH); 2) linoleic acid micelles were employed to investigate the lipid radical scavenging capacity of lactate; and 3) primary rat hepatocyte culture was used to study the inhibition of membrane lipid peroxidation by lactate. EPR experiments exhibited scavenging activities of lactate toward both O(2)(-). and.OH; lactate was also able to inhibit lipid peroxidation of hepatocyte culture. Both effects of lactate were concentration dependent. However, no inhibition of lipid peroxidation by lactate was observed in the micelle model. These results suggested that lactate ion may prevent lipid peroxidation by scavenging free radicals such as O(2)(-). and.OH but not lipid radicals. Thus lactate ion might be considered as a potential antioxidant agent.
Groussard
C
C
Laboratoire de Physiologie et de Biomécanique de l'Exercice Musculaire, UFRAPS Rennes 2, UPRES A 1274, Campus la Harpe, CS 24414, 35044 Rennes Cedex, France. carole.groussard@uhb.fr
Morel
I
I
Chevanne
M
M
Monnier
M
M
Cillard
J
J
Delamarche
A
A
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology*; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000494:pharmacology*; D019787:Linoleic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000166:cytology; D008823:Micelles; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D016166:Free Radical Scavengers; D008823:Micelles; D013481:Superoxides; D019344:Lactic Acid; D019787:Linoleic Acid