The arabidopsis DELAYED DEHISCENCE1 gene encodes an enzyme in the jasmonic acid synthesis pathway.
delayed dehiscence1 is an Arabidopsis T-DNA mutant in which anthers release pollen grains too late for pollination to occur. The delayed dehiscence1 defect is caused by a delay in the stomium degeneration program. The gene disrupted in delayed dehiscence1 encodes 12-oxophytodienoate reductase, an enzyme in the jasmonic acid biosynthesis pathway. We rescued the mutant phenotype by exogenous application of jasmonic acid and obtained seed set from previously male-sterile plants. In situ hybridization studies showed that during the early stages of floral development, DELAYED DEHISCENCE1 mRNA accumulated within all floral organs. Later, DELAYED DEHISCENCE1 mRNA accumulated specifically within the pistil, petals, and stamen filaments. DELAYED DEHISCENCE1 mRNA was not detected in the stomium and septum cells of the anther that are involved in pollen release. The T-DNA insertion in delayed dehiscence1 eliminated both DELAYED DEHISCENCE1 mRNA accumulation and 12-oxophytodienoate reductase activity. These experiments suggest that jasmonic acid signaling plays a role in controlling the time of anther dehiscence within the flower.
Sanders
P M
PM
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles 90095-1606, USA.
Lee
P Y
PY
Biesgen
C
C
Boone
J D
JD
Beals
T P
TP
Weiler
E W
EW
Goldberg
R B
RB
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D003517:Cyclopentanes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D054883:Oxylipins; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D003517:Cyclopentanes; D004269:DNA, Bacterial; D054883:Oxylipins; D012333:RNA, Messenger; C036483:T-DNA; C011006:jasmonic acid; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C106360:12-oxophytodienoate reductase