Atomic force microscopy studies of intercalation-induced changes in plasmid DNA tertiary structure.
Structural transitions in the tertiary structure of plasmid DNA have been investigated using atomic force microscopy. Changes in superhelical stress were induced by ethidium bromide intercalation, and conformational effects monitored by recording topographic images from DNA complexes of various ethidium bromide:base pair stoichiometry. Significant changes in the tertiary structure of individual DNA molecules were observed with increasing ethidium bromide concentration. The first distinct conformational transition was from a predominantly relaxed structure to one consisting solely of toroidal supercoils. A further increase in ethidium bromide concentration resulted in the formation of regions of plectonemic supercoiling. The ratio of plectonemic:toroidal supercoiling gradually increased until an extremely tightly interwound structure of solely plectonemic supercoiling was finally adopted. The toroidal form of supercoiling observed in this study is unusual as both atomic force microscopy and electron microscopy techniques have previously shown that plectonemic supercoiling is the predominant form adopted by plasmid DNA.
Pope
L H
LH
Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
Davies
M C
MC
Laughton
C A
CA
Roberts
C J
CJ
Tendler
S J
SJ
Williams
P M
PM
D004278:DNA, Superhelical / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D010957:Plasmids
D004278:DNA, Superhelical; D007364:Intercalating Agents; D004996:Ethidium