Leukocyte CD11b expression is not essential for the development of atherosclerosis in mice.
CD11b is an alpha chain of the leukocyte beta(2)-integrin, Mac-1, which mediates binding and extravasation of leukocytes. Because this event is critical in atherosclerosis, we examined the role of CD11b in lesion formation. Atherosclerosis-susceptible, low density lipoprotein receptor-deficient (LDL-R(-/)-) mice were irradiated and repopulated with bone marrow cells from CD11b-deficient (CD11b(-/)-) mice. After 4 weeks, <2% of the peripheral blood leukocytes of the CD11b(-/)- bone marrow-transplanted LDL-R(-/)- mice expressed CD11b, whereas approximately 25% of the CD11b(+/)+ bone marrow-transplanted LDL-R(-/)- mice expressed CD11b. After consuming a high-fat diet for 16 weeks the mean lesion aortic valve area, cholesterol accumulation in the aorta, and the degree of intimal macrophage infiltration were similar in mice reconstituted with either CD11b(+)(/+) or CD11b(-/)- bone marrow cells. The studies confirm that CD11b expression of bone marrow-derived cells does not influence the development of atherosclerosis in hypercholesterolemic LDL-R(-/)- mice.
Kubo
N
N
Departments of Immunology, Scripps Research Institute, La Jolla, CA 92037, USA.
Boisvert
W A
WA
Ballantyne
C M
CM
Curtiss
L K
LK
D000818:Animals; D001011:Aorta / Q000737:chemistry; D001021:Aortic Valve / Q000473:pathology; D001161:Arteriosclerosis / Q000209:etiology*; D016026:Bone Marrow Transplantation; D018821:CD18 Antigens / Q000235:genetics*; D006937:Hypercholesterolemia / Q000235:genetics*; D007962:Leukocytes / Q000502:physiology; D016177:Macrophage-1 Antigen / Q000235:genetics*; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D011828:Radiation Chimera; D011973:Receptors, LDL / Q000235:genetics*
D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D011973:Receptors, LDL