title: Fluorogenic substrates for assay of chymosin.
abstract: The use of fluorogenic substrates with intramolecular fluorescence quenching as substrates for chymosin was studied. It was shown that chymosin hydrolyzes the Phe-Phe peptide bond. The effect of pH on the hydrolysis of substrates by chymosin was investigated. The catalytic characteristics of the hydrolysis of the fluorogenic substrates were obtained at the pH optima. The influence of dimethylformamide on chymosin activity was studied.
authors.affiliation: Department of Natural Compounds Chemistry, School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. violst@genebee.msu.su.
authors.forename: V V
authors.initials: VV
authors.lastname: Starovoitova
authors.forename: I Y
authors.initials: IY
authors.lastname: Filippova
authors.forename: E N
authors.initials: EN
authors.lastname: Lysogorskaya
authors.forename: E S
authors.initials: ES
authors.lastname: Oksenoit
authors.forename: G I
authors.initials: GI
authors.lastname: Lavrenova
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D012085:Chymosin / Q000032:analysis* / Q000378:metabolism; D004126:Dimethylformamide / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D013379:Substrate Specificity
chemical_list: D005456:Fluorescent Dyes; D004126:Dimethylformamide; D012085:Chymosin