AFLP fingerprint analysis of Bradyrhizobium strains isolated from Faidherbia albida and Aeschynomene species.
The diversity of Bradyrhizobium isolates from Faidherbia albida and Aeschynomenee species was assessed using AFLP analysis, a high-resolution genomic fingerprinting technique. Reference strains from Bradyrhizobium japonicum, Bradyrhizobium elkanii and Bradyrhizobium liaoningense were included for comparison. At a similarity level of 50%, a total of 34 different groups were obtained by cluster analysis of the genomic fingerprints. Four of these clusters correspond to the three reference species, demonstrating the large diversity of the isolates studied. Comparison with other data demonstrates that AFLP has a higher resolution than restriction analysis of 16S rRNA genes, SDS-PAGE analysis of proteins and phenotypic analysis. Results of the latter two methods showed little correspondence with the genotypic data.
Willems
A
A
Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, Belgium. anne.willems@rug.ac.be
Doignon-Bourcier
F
F
Coopman
R
R
Hoste
B
B
de Lajudie
P
P
Gillis
M
M
D000045:Acacia / Q000382:microbiology*; D020369:Bradyrhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D007887:Fabaceae / Q000382:microbiology*; D014644:Genetic Variation; D016680:Genome, Bacterial; D005838:Genotype; D010946:Plants, Medicinal; D012150:Polymorphism, Restriction Fragment Length