Solid-phase peptide synthesis in the reverse (N --> C) direction.
[reaction: see text] A new strategy for SPPS in the reverse direction based on the use of 2-Cl-trityl resin, an allyl ester as the temporary protecting group, and Cu(OBt)(2)/DIPCDI or HATU/DIEA as the coupling method is described. These conditions ensure good yields with minimal racemization of the C-terminal residue.
Thieriet
N
N
Department of Organic Chemistry, University of Barcelona, 08028-Barcelona, Spain.
Guibé
F
F
Albericio
F
F
D001671:Biochemistry / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis*
D010455:Peptides