title: The antiadhesive agent sodium hyaluronate increases the proliferation rate of human peritoneal mesothelial cells.
abstract: OBJECTIVE
  Evaluating the influence of sodium hyaluronate on the proliferation rate of attached and nonattached human peritoneal mesothelial cells to elucidate whether this is a possible mechanism of action in peritoneal tissue repair.
  
  
  METHODS
  Controlled laboratory experiment.
  
  
  METHODS
  A university hospital.
  
  
  METHODS
  Five patients undergoing colorectal surgery for noninfectious reasons.
  
  
  METHODS
  Human peritoneal mesothelial cells were harvested from patients undergoing a laparotomy for noninfectious reasons. Cells, both nonattached and attached, were incubated for 4 and 24 hours with different concentrations of sodium hyaluronate. Thereafter, the cell proliferation rate was measured by XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-car boxanilide) colorimetric assay. To mimic peritoneal injury, the cells were exposed to tumor necrosis factor alpha and/or lipopolysaccharide and were incubated immediately or after 24 hours of exposure to 0% or 0.2% sodium hyaluronate. Afterward, the cell proliferation rate was measured.
  
  
  METHODS
  Proliferation rate measured by XTT assay.
  
  
  RESULTS
  Sodium hyaluronate significantly increased the proliferation rate of mesothelial cells, both in a nonattached (P<.005) and attached (P<.001) state. Exposure of the mesothelial cells to tumor necrosis factor alpha and/or lipopolysaccharide diminished the cells' proliferation rate. However, incubation of these exposed cells with 0.2% sodium hyaluronate significantly increased the proliferation rate, regardless of whether the sodium hyaluronate was added immediately (P<.001) or after 24 hours (P<.001).
  
  
  CONCLUSIONS
  Sodium hyaluronate increases the proliferation rate of human peritoneal mesothelial cells, both attached and nonattached, under normal conditions and after stimulation with tumor necrosis factor alpha and/or lipopolysaccharide.
authors.affiliation: Sahlgrenska University Hospital/Ostra, Göteborg, Sweden. mmpj.reijnen@worldonline.nl
authors.forename: M M
authors.initials: MM
authors.lastname: Reijnen
authors.forename: P
authors.initials: P
authors.lastname: Falk
authors.forename: H
authors.initials: H
authors.lastname: van Goor
authors.forename: L
authors.initials: L
authors.lastname: Holmdahl
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology*; D010533:Peritoneal Lavage; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D018960:Hyaluronan Receptors; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D006820:Hyaluronic Acid