Rapid screening of natural products by high-resolution high-temperature gas chromatography.
The crude ethanol extracts from the leaves of three Croton hemiargyrus hemiargyreus plants are fractionated by thin-layer chromatography, yielding five fractions. The fractions and the crude extract are analyzed by high-temperature high-resolution gas chromatography coupled with mass spectrometry (HT-HRGC-MS). Several natural products, including thermolabile components, can be characterized directly in the samples, such as alkaloids, terpenes, flavonoids, acids, alcohols, etc. The cold on-column technique proves to be appropriate for the injection of these thermolabile compounds. HT-HRGC-MS is shown to be a valuable tool and an alternative technique to classical phytochemical procedures for the simple and fast routine analysis of natural products in crude extracts.
Carbonell
S A
SA
LADETEC, Instituto de Química, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Cidade Universitaria, Rio de Janeiro, Brazil.
de Aquino Neto
F R
FR
Cardoso
J N
JN
dos Santos Pereira
A
A
Amaral
A C
AC
Barnes
R A
RA
D000438:Alcohols / Q000032:analysis; D000470:Alkaloids / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D002855:Chromatography, Thin Layer; D000431:Ethanol; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006358:Hot Temperature; D006838:Hydrocarbons / Q000032:analysis; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D013729:Terpenes / Q000032:analysis
D000438:Alcohols; D000470:Alkaloids; D006838:Hydrocarbons; D010936:Plant Extracts; D013729:Terpenes; D000431:Ethanol