[The expression patterns of genes in different biotypes by genome addition].
The patterns of SOD, EST and haemoglobin genes expression among the different combinations conducted with genome addition were investigated. The erythrocyte expression of these isozymes and protein were visualized by histochemical staining after polyacrylamide gradient gel electrophoresis. The results of our electrophoresis analysis have revealed that biochemical polymorphism exists in the combinations, and these genetic variance have resulted from the contribution of different genomes. This study provides additional evidence that gene dosages can be interpreted from the enzyme patterns and electrophoretic staining intensities existed in different ploidies, which suggests that subunit interactions for SOD are co-operative and additive. This would indicate that each allele could be differentially regulated so that even one-third genome can be expressed in triploid individuals. These genetic data could be used to construct genome specific varieties, and would not only help to maximize efficiency in the use of different biotypes identification, but also help to maximize studying the relationship between gene dosage, expression and regulation.
Wu
Y P
YP
Institute of Genetics, Maternal and Child Health Hospital of Zhuhai City, China.
Ye
Y Z
YZ
Wu
Q J
QJ
D000818:Animals; D018628:Gene Dosage; D015870:Gene Expression; D006454:Hemoglobins / Q000235:genetics; D006801:Humans; D011110:Polymorphism, Genetic; D013482:Superoxide Dismutase / Q000235:genetics
D006454:Hemoglobins; D013482:Superoxide Dismutase