Vitellin and vitellogenin in the soldier bug, Podisus maculiventris: identification with monoclonal antibodies and reproductive response to diet.
A 171,000 M(r )polypeptide of Podisus maculiventris (Say) (Heteroptera: Pentatomidae) that constituted 16% of the protein in eggs also constituted up to 25% of the protein in hemolymph of fed females. It was identified as the major or sole apoprotein of vitellogenin. Eggs contained major polypeptides of 171, 106, and 51 kDa. The hemolymph polypeptide was identified with a polypeptide (vitellin) in egg extracts by comparing molecular weights, specificity of occurrence in fed females, and immunological reactivities. Females, starved for 5 days after eclosion to assure complete previtellogenic development, produced vitellogenin within a day after feeding on larval Galleria mellonella, and within 4 days after feeding on an artificial diet. Appearance of vitellogenin preceded ovarian growth by 2-3 days. Two monoclonal antibodies raised against egg proteins of P. maculiventris were selected for their strong reaction against egg extract and female hemolymph and null reaction against male hemolymph. Only one 170-kDa band in egg and hemolymph reacted with the antibodies on denaturing Western blots. These monoclonal antibodies are being used to develop an enzyme-linked immunosorbent assay (ELISA) to quantitate reproductive response of females to diets of differing quality.
Shapiro
J P
JP
USDA, ARS, CMAVE, Gainesville, FL 32608, USA. jshapiro@gainesville.usda.ufl.edu
Wasserman
H A
HA
Greany
P D
PD
Nation
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004032:Diet; D004527:Egg Proteins / Q000032:analysis* / Q000276:immunology; D005260:Female; D020062:Heteroptera / Q000737:chemistry*; D008297:Male; D012098:Reproduction / Q000502:physiology; D014819:Vitellogenins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004527:Egg Proteins; D014819:Vitellogenins