title: Enzymatic degradation of cell wall polysaccharides from mango (Mangifera indica L.) puree.
abstract: Ripe mango puree (Smith cultivar) was treated with fungal polysaccharidases containing pectinolytic, hemicellulolytic, and cellulolytic activities for 2 h at 50 degrees C. A loss of 30% of the cell wall material (CWM) was measured. CWM polysaccharides were hydrolyzed to varying degrees: 88, 65, and 65% of, respectively, galacturonic acid-, arabinose-, and rhamnose-containing polymers were hydrolyzed, whereas 50% of cellulose was degraded. After 30 min of treatment, the ethanol precipitation test on the serum was negative, indicating that pectic substances were rapidly hydrolyzed. Oligogalacturonic acids (degree of polymerization, 1-12) were observed in the serum. A viscosity drop of 90% was measured after 2 h, confirming the dominant role of pectic substances in puree viscosity.
authors.affiliation: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Départment FLHOR, Montpellier, F-34032, France. olle@cirad.fr
authors.forename: D
authors.initials: D
authors.lastname: Ollé
authors.forename: A
authors.initials: A
authors.lastname: Baron
authors.forename: Y F
authors.initials: YF
authors.lastname: Lozano
authors.forename: J M
authors.initials: JM
authors.lastname: Brillouet
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D014783:Viscosity
chemical_list: D011134:Polysaccharides; D006026:Glycoside Hydrolases