title: Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay.
abstract: Canine parvovirus (CPV) was first isolated in 1978 in the USA. Analysis of CPV isolates by monoclonal antibodies and restriction enzymes have shown that after the first emergence of CPV (CPV-2) it evolved to give rise to new antigenic types, which were designated CPV type 2a and type 2b. These new types have replaced the original CPV type 2, although the proportions of each of the new antigenic types vary in different countries. In Brazil, CPV-like infections were first observed in 1979, however, there has been no information concerning the antigenic types of CPV prevailing in South America. In this study, we designed a PCR assay to type canine parvovirus strains in fecal samples collected from symptomatic dogs during 1980 through 1986 and 1990 through 1995. Our data showed that the CPV epizootic in Brazil followed the same pattern observed in the USA of emergence of CPV-2 followed by replacement by the variants CPV-2a and 2b. The predominant strain found during 1980 was CPV-2a, which was substantially replaced by CPV-2b from 1990 to 1995.
authors.affiliation: Institute of Biomedical Sciences, University of São Paulo, 1374, Av. Prof. Lineu Prestes, CEP 05508-900, São Paulo, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Pereira
authors.forename: T A
authors.initials: TA
authors.lastname: Monezi
authors.forename: D U
authors.initials: DU
authors.lastname: Mehnert
authors.forename: M
authors.initials: M
authors.lastname: D'Angelo
authors.forename: E L
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D000818:Animals; D000940:Antigenic Variation / Q000235:genetics; D001483:Base Sequence; D001938:Brazil; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005243:Feces / Q000821:virology; D006386:Hemagglutination Tests / Q000662:veterinary; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D004279:DNA, Viral