A simple method for taking photographs of histological sections without using neither photographic camera nor microscope.
A simple photographic method for histological sections is described. This is a contact method which does not require the use of photographic camera. It is very similar to the contact print method used routinely in photographic laboratories. It is performed by placing the slides with the histological section, coverslip downwards, over the emulsion of the negative film. Then, they are illuminated with a common photographic enlarger during a brief period of time. Thus, a negative contact print of the section is obtained. This technique yields photographs of high quality focus, sharply contrasted with great detail. These results may also be obtained with low-contrast stained sections. This method is most suitable for photography of large or medium-sized histological sections (macros). This is often the case of neuroanatomical and immunohistochemical studies. Our method, being simple and quick, is recommended for routine use in laboratories.
Carmanchahi
P D
PD
Instituto de Neurociencia (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina. PDC@biolo.bg.fcen.uba.ar
Aldana Marcos
H J
HJ
Ferrari
C C
CC
Affanni
J M
JM
D006652:Histological Techniques; D010784:Photomicrography / Q000379:methods*