title: Semiquantitative morphological analysis of stromal cells in the irradiated and recovering rat thymus.
abstract: To understand the roles of thymic stromal cells in T-lymphocyte development, we semiquantitatively analysed rat thymi recovering from irradiation (6 Gy), using a transmission electron microscope. The most striking findings were that the percentage of subcapsular epithelial cells significantly increased in the cortex on day 3 after irradiation compared with the control; the percentage of intermediate epithelial cells significantly increased in the cortex on days 3 and 5 after irradiation and in the medulla on days 5 and 7 compared with the control; the interdigitating cells disappeared from the medulla by day 7 after irradiation and reappeared on day 9. The present data thus reveal that during recovery after irradiation (6 Gy), marked changes occur in the relative proportions of different epithelial cell subtypes in the cortex and medulla of the rat thymus. In addition, the percentages of macrophages and interdigitating cells also changed during the recovery. These changes, which may be associated with the abrupt proliferation of thymocytes after irradiation, should shed light on the significance of stromal cells in the T cell development.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Arudchelvan
authors.forename: N
authors.initials: N
authors.lastname: Tokuda
authors.forename: M
authors.initials: M
authors.lastname: Tamechika
authors.forename: Y H
authors.initials: YH
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Mizutani
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Fukumoto
authors.forename: F
authors.initials: F
authors.lastname: Shinozaki
mesh_terms: D000818:Animals; D005260:Female; D051381:Rats; D017154:Stromal Cells / Q000528:radiation effects / Q000648:ultrastructure; D013950:Thymus Gland / Q000473:pathology / Q000528:radiation effects / Q000648:ultrastructure*; D014916:Whole-Body Irradiation