title: Reliable gas chromatographic-mass spectrometric method combined with a headspace autosampler for isoflurane determination in blood.
abstract: Isoflurane is a nonflammable, liquid, volatile inhalation anesthetic administered by vaporizing. Although it is now commonly used, fatal cases resulting from its abuse or misuse have been reported. A combined system of a gas chromatograph-mass spectrometer and a headspace autosampler is therefore proposed for the detection of blood isoflurane. This analytic method showed sharp and well separated peaks, and revealed a good linear relationship (r=0.9994) with a function of y = 7.3768x - 0.0222 at concentrations between 18.7 and 299.2 microg/ml. The limits of detection and quantitation of this method were 1.2 and 4.7 microg/ml, respectively. The within- and between-run precision for spiked samples, assessed by the coefficient of variations, ranged from 1.7 to 10.0% and from 4.1 to 12.8%, respectively. The within- and between-run accuracy, assessed by errors from theoretical values, were 2.2-7.8% and 2.4-9.6%, respectively. In addition, practical sample analysis showed a good applicability, with a within-run precision rate of 5.6 to 7.7% and a between-run precision rate of 5.2-10.6%. In summary, the present work presents a valid alternative for blood isoflurane analysis.
authors.affiliation: Department of Anesthesiology, Taichung Veterans General Hospital, Taiwan.
authors.forename: N C
authors.initials: NC
authors.lastname: Yang
authors.forename: K L
authors.initials: KL
authors.lastname: Hwang
authors.forename: D Z
authors.initials: DZ
authors.lastname: Hung
authors.forename: H H
authors.initials: HH
authors.lastname: Wuhh
authors.forename: W M
authors.initials: WM
authors.lastname: Ho
mesh_terms: D018685:Anesthetics, Inhalation / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007530:Isoflurane / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D018685:Anesthetics, Inhalation; D007530:Isoflurane