Novel HLA-B*15 alleles identified in potential marrow donors.
Several methods for low-resolution class I typing of potential bone marrow donors are available. The National Marrow Donor Program (NMDP) has initiated pilot projects for large-scale DNA-based class I typing to initially characterize donors. Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) screening of 3,500 NMDP potential donors suggested the presence of variants of known HLA-B*15 variants in 3 donors. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. Sequencing revealed 3 alleles differing from known HLA-B*15 alleles by nucleotide substitutions resulting in predicted novel HLA-B antigens. The new alleles occur in distinct ethnic groups. These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations.
Williams
T M
TM
Department of Pathology, University of New Mexico, Albuquerque 87131-5051, USA. twilliams@salud.unm.edu
Wu
J
J
Bassinger
S
S
Montoya
G D
GD
Yee
J
J
Griffith
B B
BB
Kearns
J
J
McKeen
M
M
Birkos
S
S
Gourley
I S
IS
Kamoun
M
M
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005091:Exons / Q000276:immunology; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D059910:HLA-B15 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D014019:Tissue Donors; D014481:United States; D001741:Black or African American
D015235:HLA-B Antigens; D059910:HLA-B15 Antigen