[Weight distribution and optical characteristics of soil humic acids fractionated by gel chromatography and electrophoresis].
Combination of gel chromatography on Sephadex and polyacrylamide gel electrophoresis was used to obtain molecular weight- and electrophoretic mobility-uniform fractions of humic acids from soil of different types (chernozem, sod-podzol, grey forest, and red soils). Extinction and color (E4/E6) coefficients, as well as weight distribution (%) were studied for all humic acids and their fractions. The fractions differed by the weight distribution (%). Sod-podzol and red soils had twice the amount of high-molecular and half the amount of low molecular HA fractions as compared to chernozem and grey forest soil.
Trubetskoĭ
O A
OA
Institute of Soil Science and Photosynthesis, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
Trubetskaia
O E
OE
Reznikova
O I
OI
Afanas'eva
G V
GV
D005591:Chemical Fractionation; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006812:Humic Substances / Q000032:analysis*; D012987:Soil / Q000032:analysis*
D006812:Humic Substances; D012987:Soil