The galactose-binding and mannose-binding jacalin-related lectins are located in different sub-cellular compartments.
A galactose-specific and a mannose-specific lectin of the family of the jacalin-related lectins have been localized by immunofluorescence microscopy. The present localization studies provide for the first time unambiguous evidence for the cytoplasmic location of the mannose-specific jacalin-related lectin from rhizomes of Calystegia sepium, which definitely differs from the vacuolar location of the galactose-specific jacalin from Artocarpus integrifolia. These observations support the hypothesis that the galactose-specific jacalin-related lectins evolved from their mannose-specific homologues through the acquisition of vacuolar targeting sequences.
Peumans
W J
WJ
Laboratory for Phytopathology, Katholieke Universiteit Leuven, Willen de Croylaan 42, 3001 Leuven, Belgium.
Hause
B
B
Van Damme
E J
EJ
D002451:Cell Compartmentation; D005690:Galactose / Q000378:metabolism*; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D037121:Plant Lectins; D011485:Protein Binding; D013347:Subcellular Fractions / Q000378:metabolism*
D037102:Lectins; D037121:Plant Lectins; C043696:jacalin; D008358:Mannose; D005690:Galactose