Detection of group- and subgroup-specific antigens of bovine rotaviruses in Bangladesh.
The study was carried out to detect group- and subgroup-specific antigens of bovine rotaviruses. Stool specimens, collected from diarrhoeic calves of the Savar Dairy Farm, Bangladesh, were examined by an enzyme-linked immunosorbent assay, using group- and subgroup-specific monoclonal antibodies. Thirty-three specimens showed specificity for group A rotavirus. While subgrouping, 21 group A-positive specimens showed subgroup I specificity. Twelve specimens did not react with either of the subgroup I- and subgroup II-specific monoclonal antibodies.
Alam
M M
MM
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Ahmed
M U
MU
Chowdhury
N S
NS
Urasawa
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000302:isolation & purification*; D001459:Bangladesh; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D012401:Rotavirus / Q000276:immunology* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral