Effect of exercise training on erythrocyte insulin-like growth factor-I receptor binding in adolescent males.
Exercise training leads to tissue anabolism by acting through the growth hormone (GH)-insulin-like growth factor-I (IGF-I) axis, but the role of tissue IGF-I receptors in this process is not known. Erythrocyte IGF-I receptor binding is known to be affected by circulating levels of IGF-I. We hypothesized that training would lead to alterations in erythrocyte IGF-I binding. Thirty-eight adolescent males (mean age 16+/-0.7 yr) were randomized to a control (n=18) or endurance training intervention lasting 5 weeks. Erythrocyte IGF-I binding was measured by standard techniques. Quantity of receptor binding sites (R), binding affinity constant (Kaff), and nonspecific binding (NS) were calculated. Training led to increases (p<0.05) in 1) Kaff x R - an index of overall binding capacity (control, 7.3 +/-8.0%; trained, 60+/-21%); and 2) NS (control, 1.5+/-6.6%; trained 23.2+/-7.5%). Kaff, R, and R/erythrocyte increased in trained subjects, but not significantly so. These results suggest a training-associated upregulation of IGF-I binding. Finally, the unexpected increase in NS may reflect IGF binding protein activity, rather than changes in the erythrocyte IGF-I receptors themselves.
Lee
C
C
Department of Pediatrics, Harbor UCLA-Medical Center, Torrance, CA, USA.
Eliakim
A
A
Brasel
J A
JA
Cooper
D M
DM
D000293:Adolescent; D004912:Erythrocytes / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D006801:Humans; D008297:Male; D010807:Physical Endurance; D017526:Receptor, IGF Type 1 / Q000097:blood*
D017526:Receptor, IGF Type 1