title: Ascorbate oxidation is a prerequisite for its transport into rat liver microsomal vesicles.
abstract: Oxidation and uptake of ascorbate show similar time courses in rat liver microsomal vesicles: a rapid burst phase is followed by a slower process. Inhibitors of ascorbate oxidation (proadifen, econazole or quercetin) also effectively decreased the uptake of ascorbate. The results show that dehydroascorbate is the transport form of ascorbate at the membrane of the endoplasmic reticulum.
authors.affiliation: Department of Medical Chemistry, Semmelweis University, P.O.B. 260, H-1444 Budapest, Hungary.
authors.forename: M
authors.initials: M
authors.lastname: Csala
authors.forename: V
authors.initials: V
authors.lastname: Mile
authors.forename: A
authors.initials: A
authors.lastname: Benedetti
authors.forename: J
authors.initials: J
authors.lastname: Mandl
authors.forename: G
authors.initials: G
authors.lastname: Bánhegyi
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001692:Biological Transport; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001205:Ascorbic Acid