Comparative evaluation of a field-based dot-ELISA kit with three other serological tests for the detection of Brucella antibodies in goats.
A dot-ELISA (d-ELISA) test was evaluated and compared with the serum agglutination test (SAT), micro-complement fixation test (CFT) and a plate-ELISA (p-ELISA) for field use in screening herds of goats against brucellosis. During the standardization of the dot-ELISA kit on 1732 caprine serum samples, 1571 samples out of 1666 were found to be negative in d-ELISA, SAT and micro-CFT, while 59 were positive in different combinations. Of a further 66 serum samples, 34 were negative and 31 were positive in different combinations in d-ELISA, SAT, micro-CFT and p-ELISA. A total of 1584 goats belonging to different herds were then screened for brucellosis. Of the 694 serum samples screened in the first batch using d-ELISA, a positive reaction was observed in 26 cases. Further screening of these cases revealed 13 and 21 goats as positive reactors in SAT and CFT, respectively. In a second batch of 890 goats there were 109 positive reactors in d-ELISA. Among these 109 goats, 34, 40 and 80 goats were positive reactors in SAT, CFT and p-ELISA, respectively. The results of d-ELISA correlated well with those of p-ELISA. Dot-ELISA was found to be a more suitable and rapid test for screening large numbers of goats in the field.
Singh
S V
SV
Goat Health Division, Central Institute for Research on Goats, Makhdoom, Mathura, UP, India. shoorvir@cirg.up.nic.in
Gupta
V K
VK
Singh
N
N
D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002002:Brucella / Q000276:immunology* / Q000302:isolation & purification; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D003168:Complement Fixation Tests / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D015511:Goat Diseases / Q000175:diagnosis* / Q000382:microbiology; D006041:Goats; D011933:Reagent Kits, Diagnostic / Q000662:veterinary
D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic