Participation of Poly(ADP-ribose)-polymerase of nuclear matrix in DNA repair.
The poly(ADP-ribose)-polymerase activity of brain and liver cell nuclei is changed during X-irradiation of rats. In the nuclear matrix, poly(ADP-ribose)-polymerase activity increases at a low dose of irradiation (1.7 Gy) and decreases at a high dose (6.7 Gy). A significant part of the activity of nuclear NMN-adenylyltransferase, a key enzyme for biosynthesis of NAD (the substrate of poly(ADP-ribose)-polymerase), has been found in the nuclear matrix. An interrelation between ADP-ribosylation taking place on the matrix level and eukaryotic cell DNA repair is suggested.
Zaalishvili
T M
TM
Institute of Molecular Biology and Biological Physics, Georgian Academy of Sciences, Tbilisi, 380060, Georgia.
Gabriadze
I Y
IY
Margiani
D O
DO
Philauri
V R
VR
Surguladze
N M
NM
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000528:radiation effects; D004260:DNA Repair; D008099:Liver / Q000201:enzymology / Q000528:radiation effects; D015530:Nuclear Matrix / Q000201:enzymology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D014965:X-Rays
D011065:Poly(ADP-ribose) Polymerases