title: Cantaloupe melon peroxidase: characterization and effects of additives on activity.
abstract: Peroxidase in cantaloupe melon (Cucumis melo L. var. reticulatus Naud.), a fruit commonly fresh cut processed, was characterized to determine reaction pathway, optimal conditions for activity and effect of some additives on enzymatic action. Mn2+, CaCl2, NaNO2 and kinetin had partial inhibitory effects on enzyme activity. Activity was effectively inhibited by compounds capable of chelating peroxidase heme iron such as diethyldithiocarbamate and tiron, but unaffected by EDTA. Free radical scavenger, superoxide dismutase, also had no effect on reaction velocity. Enzymatic action was consistent with that of ascorbate peroxidase based on the relatively higher affinity for ascorbate over guaiacol. Optimum activity temperature was 50-55 degrees C. The enzyme was stable at temperatures below 40 degrees C and at 50 degrees C for up to 10 min. Over 90% of total activity was lost at 80 degrees C within 5 min. Broad pH optima, 5.5-7.5 at 50 degrees C and 6-7 at 30 degrees C, were obtained. Peroxidase activity in cantaloupe was higher than those in strawberry (Fragaria ananassa Duch.) and lettuce (Lactuca sativa L.), suggesting a relatively high oxidative stress in fresh cut cantaloupe. The potential use of ascorbate as an additive in fresh cut cantaloupe melon was demonstrated by its ability to preserve color in minimally processed fruits for 25 days at 4 degrees C, possibly as a result of an enhanced antioxidative action of the ascorbate-peroxidase complex and trace metal ion cofactors.
authors.affiliation: United States Department of Agriculture, Southern Regional Research Center, New Orleans, LA 70124, USA. sola@nola.srrc.usda.gov
authors.forename: O
authors.initials: O
authors.lastname: Lamikanra
authors.forename: M A
authors.initials: MA
authors.lastname: Watson
mesh_terms: D003116:Color; D005503:Food Additives / Q000737:chemistry*; D005519:Food Preservation; D005638:Fruit / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D009195:Peroxidase / Q000737:chemistry*; D013696:Temperature
chemical_list: D005503:Food Additives; D009195:Peroxidase