title: Characterization of sulfate assimilation in marine algae focusing on the enzyme 5'-adenylylsulfate reductase.
abstract: 5'-Adenylylsulfate (APS) reductase was characterized in diverse marine algae. A cDNA encoding APS reductase from Enteromorpha intestinalis (EAPR) was cloned by functional complementation of an Escherichia coli cysH mutant. The deduced amino acid sequence shows high homology with APS reductase (APR) from flowering plants. Based on the probable transit peptide cleavage site the mature protein is 45.7 kD. EAPR expressed as a His-tagged recombinant protein catalyzes reduced glutathione-dependent reduction of APS to sulfite, exhibiting a specific activity of approximately 40 micromol min(-1) mg protein(-1) and Michealis-Menten kinetic constants of approximately 1.4 mM for reduced glutathione and approximately 6.5 microM for APS. APR activity and expression were studied in relation to the production of 3-dimethylsulfoniopropionate (DMSP), a sulfonium compound produced by many marine algae. A diverse group of DMSP-producing species showed extremely high enzyme activity (up to 400 times that found in flowering plants). Antibodies raised against a conserved peptide of APR strongly cross-reacted with a protein of 45 kD in several chlorophytes but insignificantly with chromophytes. In the chlorophyte Tetraselmis sp., APR activity varies significantly during the culture cycle and does not follow the changes in cellular DMSP content. However, a positive correlation was found between cell-based APR activity and specific growth rate.
authors.affiliation: Biotechnology Center for Agriculture and the Environment and Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08901-8520, USA. gao@aesop.rutgers.edu
authors.forename: Y
authors.initials: Y
authors.lastname: Gao
authors.forename: O M
authors.initials: OM
authors.lastname: Schofield
authors.forename: T
authors.initials: T
authors.lastname: Leustek
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D000460:Chlorophyta / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism*; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010839:Phytoplankton / Q000201:enzymology; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013452:Sulfonium Compounds / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D013452:Sulfonium Compounds; C068078:dimethylpropiothetin; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase