title: Cutting edge: cell autonomous rather than environmental factors control bacterial superantigen-induced T cell anergy in vivo.
abstract: Anergic T cells display a marked decrease in their ability to produce IL-2 and to proliferate in the presence of an appropriate antigenic signal. Two nonmutually exclusive classes of models have been proposed to explain the persistence of T cell anergy in vivo. While some reports indicate that anergic T cells have intrinsic defects in signaling pathways or transcriptional activities, other studies suggest that anergy is maintained by environmental "suppressor" factors such as cytokines or Abs. To distinguish between these conflicting hypotheses, we employed the well-characterized bacterial superantigen model system to evaluate in vivo the ability of a trace population of adoptively transferred naive or anergized T cells to proliferate in a naive vs anergic environment upon subsequent challenge. Our data clearly demonstrate that bacterial superantigen-induced T cell anergy is cell autonomous and independent of environmental factors.
authors.affiliation: Ludwig Institute for Cancer Research, Lausanne Branch, and Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Attinger
authors.forename: H
authors.initials: H
authors.lastname: Acha-Orbea
authors.forename: H R
authors.initials: HR
authors.lastname: MacDonald
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D017635:Clonal Anergy / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D004334:Drug Administration Schedule; D004768:Enterotoxins / Q000008:administration & dosage / Q000276:immunology*; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D016130:Immunophenotyping; D007279:Injections, Subcutaneous; D008213:Lymphocyte Activation / Q000276:immunology; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology / Q000637:transplantation; D013194:Staining and Labeling; D013211:Staphylococcus aureus / Q000276:immunology*; D013388:Succinimides / Q000378:metabolism; D018089:Superantigens / Q000008:administration & dosage / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D004768:Enterotoxins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013388:Succinimides; D018089:Superantigens; C031818:enterotoxin B, staphylococcal