The identification of novel therapeutic targets for the treatment of malignant brain tumors.
A two-step strategy was developed consisting of differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) with cultured normal human fetal astrocytes and U-373MG glioma cells followed by reverse Northern analysis of normal brain and primary tumor tissues. hu-dek, alpha-NAC, ribosomal proteins L7a and L35a, and five novel genes were identified. Since none of these genes has been previously shown to be associated with malignant brain tumor formation, this approach may be useful to identify novel targets for the diagnosis and treatment of brain tumors.
Kroes
R A
RA
The Chicago Institute of Neurosurgery and Neuroresearch, 2515 N. Clark St., Suite 800, Chicago, IL, 60614, USA.
Jastrow
A
A
McLone
M G
MG
Yamamoto
H
H
Colley
P
P
Kersey
D S
DS
Yong
V W
VW
Mkrdichian
E
E
Cerullo
L
L
Leestma
J
J
Moskal
J R
JR
D001253:Astrocytes / Q000502:physiology; D015152:Blotting, Northern; D001921:Brain / Q000502:physiology; D001932:Brain Neoplasms / Q000235:genetics*; D029721:Drosophila Proteins; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods; D005909:Glioblastoma / Q000235:genetics*; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D018832:Molecular Chaperones; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics; D036081:Receptors, Eph Family; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D029721:Drosophila Proteins; D018832:Molecular Chaperones; C489281:RPL35A protein, human; C057838:RPL7A protein, human; D012269:Ribosomal Proteins; C489186:RpL7A protein, Drosophila; C489189:Rpl7a protein, rat; D015534:Trans-Activators; C096099:nascent-polypeptide-associated complex; C119557:Dek protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases; D036081:Receptors, Eph Family