The expression of glucose regulated protein-94 in colorectal carcinoma cells treated by sodium butyrate.
The expression of glucose regulated protein 94 (GRP94) during the treatment of human colorectal carcinoma cell line-Clone A cells with sodium butyrate was studied. Sodium butyrate (SB) can cause functional and morphological effects on Clone A cells including growth arrest at G0/G1 stage and cell differentiation as observed by morphological changes, MTT and flow cytometry assays, as well as reduced Grp94 gene expression as shown by Northern blot and Western blot assays. The possible mechanism of the correlation between Grp94 gene expression and tumor growth inhibition and cell differentiation is briefly discussed.
Wu
Y D
YD
Key Laboratory of Cell Biology, Ministry of Public Health of China.
Song
J D
JD
D002087:Butyrates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D015179:Colorectal Neoplasms; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006131:Growth Inhibitors / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D018832:Molecular Chaperones / Q000096:biosynthesis / Q000235:genetics*; D012964:Sodium; D014407:Tumor Cells, Cultured
D002087:Butyrates; D006131:Growth Inhibitors; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; D018832:Molecular Chaperones; C018551:glucose-regulated proteins; D012964:Sodium; D005947:Glucose