Regulation of expression of nuclear factor kappa B RelA in oligodendrocytes: effect of hypoxia.
Little is known about the expression of nuclear factor kappa B (NF-kappaB) in oligodendrocytes. We demonstrate that under normal culture conditions only 21% of oligodendrocytes express NF-kappaB p65; exposure to hypoxia increased this to 69%. A similar increase in total p65 was seen with Western blots. Northern blot analysis demonstrated that NF-kappaB p65 mRNA increased during the first few h following hypoxia, suggesting that exposure to hypoxia affects NF-kappaB p65 transcription or p65 mRNA stability. Under normoxic conditions only 2% of the oligodendrocytes exhibited activated NF-kappaB; this was increased to 8% by hypoxia followed by reoxygenation. Despite activation of NF-kappaB, no detectable expression of the pro-inflammatory genes iNOS and ICAM-1 was seen. This is the first demonstration that hypoxia can up-regulate p65.
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Department of Anatomy and Cell Biology and The Cameco MS Neuroscience Research Centre, University of Saskatchewan, Saskatoon, Canada.
Juurlink
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D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D000860:Hypoxia / Q000378:metabolism*; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D009836:Oligodendroglia / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012016:Reference Values; D051996:Transcription Factor RelA
D016328:NF-kappa B; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D018799:Intercellular Adhesion Molecule-1; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat