Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens.
We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.
Espíndola
N M
NM
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brasil.
De Gaspari
E N
EN
Nakamura
P M
PM
Vaz
A J
AJ
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D003429:Cross Reactions; D003551:Cysticercosis / Q000276:immunology; D005260:Female; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D013621:Taenia / Q000276:immunology*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M