Effect of dietary oil intake on hepatic cytochrome P450 activity in the rat.
The objective of the present study was to investigate whether or not different dietary oils, commonly used as drug vehicles, alter hepatic microsomal drug metabolism in the rat. Male adult Sprague-Dawley rats were administered 1 mL/kg/d of either corn, olive, sesame, or soybean oil via oral gavage for 7 days. An additional rat group was given an equal volume of water each day to serve as a control. We found that the hepatic cytochrome P450 (CYP) 3A2 protein level increased by 16% (p < 0.01) in rats given soybean oil compared with control rats. In contrast, CYP2C11 protein levels decreased by 32% (p < 0.01) in the corn oil group and by 31% (p < 0. 01) in rats given olive oil. The changes in the in vitro production of 6beta- and 2alpha-hydroxytestosterone, markers of CYP3A2 and 2C11 activities, respectively, were consistent with their protein levels, although not statistically different than controls. The results demonstrate that dietary oils may have differential effects on specific hepatic CYP isoforms and may add to the variability in metabolism when xenobiotics are administered using dietary oils as vehicles.
Brunner
L J
LJ
Pharmaceutics Division, College of Pharmacy, PHR 4.214E, The University of Texas at Austin, Austin, Texas 78712-1074, USA. lane.brunner@mail.utexas.edu
Bai
S
S
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004042:Dietary Fats, Unsaturated; D003577:Cytochrome P-450 Enzyme System