Glycated apolipoprotein A-I assay by combination of affinity chromatography and latex immunoagglutination.
The degree of glycation of plasma apolipoprotein A-I was measured by a combination of gel filtration, boronate affinity chromatography and latex immunoagglutination. The plasma concentrations of apolipoprotein A-I determined by this combination method (y) correlated well with those determined by turbidimetric immunoassay (x) (y=1.12x + 1.9, r=0.964). The inter- and intra-assay coefficients of variation in the glycated apolipoprotein A-I assay were 4.1-5.0% and 4.0-4.4%, respectively. Interference from plasma glucose at concentrations up to 55.1 mmol/L was eliminated by gel filtration. Labile glycated apolipoprotein A-I did not interfere with the measurement of glycated apolipoprotein A-I. Reference values for glycated apolipoprotein A-I were determined to be 2.4-4.0% (n=140), with no significant difference between men and women. The mean concentration of plasma glycated apolipoprotein A-I in patients with uncontrolled diabetes mellitus (5.11%) was significantly higher than in normal subjects (3.12%, P<0.001). The method is simple, rapid and highly sensitive for determination of the glycation level of plasma apolipoprotein A-I.
Shishino
K
K
Department of Clinical Laboratory Medicine and Central Laboratory, School of Medicine, Ehime University, Onsen-Gun, Japan. kshishi@m.ehime-u.ac.jp
Murase
M
M
Makino
H
H
Saheki
S
S
D000371:Agglutination; D016632:Apolipoprotein A-I / Q000097:blood* / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001897:Boronic Acids / Q000378:metabolism; D016022:Case-Control Studies; D002624:Chemistry, Clinical / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D002850:Chromatography, Gel; D003920:Diabetes Mellitus / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007118:Immunoassay; D008297:Male; D012680:Sensitivity and Specificity; D012737:Sex Factors; D013997:Time Factors
D016632:Apolipoprotein A-I; D001786:Blood Glucose; D001897:Boronic Acids