The chemical synthesis of oligoribonucleotides with selectively placed 2'-O-phosphates.
A phosphoramidite, solid support method for the chemical synthesis of oligoribonucleotides containing 2'-O-phosphate at a selected position is presented. Synthesis of these oligoribonucleotides is based on uridine- and adenosine-(2'-O-phosphate)-3'-phosphoramidites, and a new condition for removal of 2'-O-phosphate protecting groups, which does not cleave internucleotide bonds. The structure of oligoribonucleotides with 2'-O-phosphate has been proven by enzymatic digestions and dephosphorylation by yeast 2'-phosphotransferase.
Kierzek
R
R
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
Steiger
M A
MA
Spinelli
S L
SL
Turner
D H
DH
Phizicky
E M
EM
D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
D009843:Oligoribonucleotides; D010770:Phosphotransferases; D000241:Adenosine; D014529:Uridine