title: Shunting is a translation strategy used by plant pararetroviruses (Caulimoviridae).
abstract: In eukaryotes standard initiation of translation involved 40S ribosome scanning to bridge the distance from the cap to the initiation codon. Recently deviations from that rule had been described, including "internal initiation", "poly-A dependent translation", and "ribosome shunting". In ribosome shunting, ribosomes start scanning at the cap but large portions of the leader are skipped. Thereby the secondary structure of the shunted region is preserved. Scanning in plant caulimoviruses involve a small open reading frame properly spaced in front of a strong stem structure, and, in order to function, the small open reading frome has to be translated and the peptide released. This arrangement can be mimicked by artificial small open reading frames and stem structures. Shunting with viral and synthetic leaders occurs not only in plant-, but also in mammalian and yeast systems. Thus it responds to an intrinsic property of the eukaryotic translational machinery and probably acts in many cases where coding regions are preceded by complex leaders.
authors.affiliation: Friedrich Miescher Institute, P.O. Box 2543, CH-4002, Basel, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Hohn
authors.forename: S
authors.initials: S
authors.lastname: Corsten
authors.forename: D
authors.initials: D
authors.lastname: Dominguez
authors.forename: J
authors.initials: J
authors.lastname: Fütterer
authors.forename: D
authors.initials: D
authors.lastname: Kirk
authors.forename: M
authors.initials: M
authors.lastname: Hemmings-Mieszczak
authors.forename: M
authors.initials: M
authors.lastname: Pooggin
authors.forename: N
authors.initials: N
authors.lastname: Schärer-Hernandez
authors.forename: L
authors.initials: L
authors.lastname: Ryabova
mesh_terms: D017796:Caulimovirus / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010448:Peptide Initiation Factors / Q000378:metabolism; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D010448:Peptide Initiation Factors; D012367:RNA, Viral