Expression of T-cell-associated antigens in B-cell non-Hodgkin's lymphoma.
We performed the immunophenotyping of 101 patients with B-cell non-Hodgkin's lymphoma (B-NHL) using two-colour flow cytometry (FCM) and found that lymphoma cells coexpressed at least one kind of T-cell-associated antigen (T-Ag; CD2, CD5, CD7) in 25 patients (24. 8%). Among these three T-Ags, CD5 was the most frequently expressed, in 21 patients (20.8%), followed by CD7, expressed in five patients (5.0%), and CD2, which was expressed in two patients (2.0%). Two kinds of T-Ag were simultaneusly expressed in three patients (CD2/CD5, CD2/CD7, and CD5/CD7, each expressed in one patient). Concerning the expression pattern of T-Ag, there were no significant differences between lymph nodes and extranodal organs in the three patients with T-Ag-positive B-NHL (T-Ag(+) B-NHL) who were analysed. When comparing the clinical features between T-Ag(+) B-NHL and T-Ag-negative B-NHL (T-Ag(-) B-NHL), extranodal involvement and higher International Prognostic Index (H and H.I.) were significantly frequent in the former subgroup (P = 0.0119 and P = 0. 0302 respectively).
Inaba
T
T
Department of Clinical Laboratory and Medicine, Kyoto Prefectural University of Medicine, Japan. inaba178@labmed.kpu-m.ac.jp
Shimazaki
C
C
Sumikuma
T
T
Okano
A
A
Hatsuse
M
M
Okamoto
A
A
Takahashi
R
R
Ashihara
E
E
Hibi
S
S
Sudo
Y
Y
Yamagata
N
N
Murakami
S
S
Rin
K
K
Fujita
N
N
Yoshimura
M
M
Nakagawa
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens / Q000032:analysis*; D019011:Antigens, CD7 / Q000032:analysis; D015139:Blotting, Southern; D018801:CD2 Antigens / Q000032:analysis; D018956:CD5 Antigens / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D019011:Antigens, CD7; D018801:CD2 Antigens; D018956:CD5 Antigens