title: Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT).
authors.affiliation: Developmental Genetics Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA. truettge@pbrc.edu
authors.forename: G E
authors.initials: GE
authors.lastname: Truett
authors.forename: P
authors.initials: P
authors.lastname: Heeger
authors.forename: R L
authors.initials: RL
authors.lastname: Mynatt
authors.forename: A A
authors.initials: AA
authors.lastname: Truett
authors.forename: J A
authors.initials: JA
authors.lastname: Walker
authors.forename: M L
authors.initials: ML
authors.lastname: Warman
mesh_terms: D000375:Aging; D000818:Animals; D002021:Buffers; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D003848:Deoxyguanine Nucleotides; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015636:Magnesium Chloride; D051379:Mice; D016133:Polymerase Chain Reaction; D012972:Sodium Hydroxide; D012995:Solubility; D019914:Taq Polymerase; D013942:Thymine Nucleotides
chemical_list: D002021:Buffers; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D003848:Deoxyguanine Nucleotides; D007202:Indicators and Reagents; D013942:Thymine Nucleotides; D015636:Magnesium Chloride; C024107:2'-deoxycytidine 5'-triphosphate; D012972:Sodium Hydroxide; C029603:deoxyguanosine triphosphate; D004247:DNA; D019914:Taq Polymerase; C026600:2'-deoxyadenosine triphosphate; C024157:thymidine 5'-triphosphate