title: Identification of major histocompatibility complex class II-associated peptides derived from freshly prepared rat Langerhans cells using MALDI-PSD and Edman degradation.
abstract: The isolation and identification is described of MHC class II-bound peptides derived from Langerhans cells. A combination of preparative micro-HPLC, MALDI-MS, Edman degradation was used for determining the amino acid sequence of MHC-associated peptides. Sample handling was crucial because fractions containing trace amounts of material require immediate storage at -80 degrees C to prevent peptide losses.
authors.affiliation: Institut für Physiologische Chemie, Ruhr-Universität Bochum, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Sickmann
authors.forename: M
authors.initials: M
authors.lastname: Blüggel
authors.forename: M
authors.initials: M
authors.lastname: Kulke
authors.forename: H
authors.initials: H
authors.lastname: Kremmin
authors.forename: A
authors.initials: A
authors.lastname: Marx
authors.forename: K
authors.initials: K
authors.lastname: Reske
authors.forename: H E
authors.initials: HE
authors.lastname: Meyer
mesh_terms: D000818:Animals; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007801:Langerhans Cells / Q000276:immunology*; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D011917:Rats, Inbred Lew; D012867:Skin / Q000276:immunology*
chemical_list: D000949:Histocompatibility Antigens Class II