Sero- and molecular typing of Duffy blood group in Southeast Asians and Oceanians.
Duffy blood group was studied among malaria-endemic Thai and Indonesian populations: Hmong (n = 103), Akha (n = 218), Lisu (n = 44), Bugis (n = 95), Toraja (n = 77), Dani (n = 44), Mee (n = 80) and Irianese (n = 81). Phenotypes were studied by the ordinal indirect Coombs' test and genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test. DNA analysis was used to type the Duffy blood group system. Deduced allele frequencies of Fya (0.958-1.0) based on the phenotypes were similar to those in other populations in Southeast Asia and Oceania. The study revealed the presence of Fya-antigens showing weak reactivity to antisera as well as a discrepancy between the genotype shown by the PCR-RFLP study and that predicted by the phenotype. The PCR-RFLP study also suggested the presence of an alternative genetic basis for the Fy(a- b-) phenotype, which differs from the African type.
Shimizu
Y
Y
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.
Ao
H
H
Soemantri
A
A
Tiwawech
D
D
Settheetham-Ishida
W
W
Kayame
O W
OW
Kimura
M
M
Nishioka
T
T
Ishida
T
T
D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D004375:Duffy Blood-Group System / Q000235:genetics*; D019353:Endemic Diseases; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D008288:Malaria / Q000097:blood* / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012494:Sampling Studies; D013785:Thailand / Q000453:epidemiology
D004375:Duffy Blood-Group System