title: Structure of a thioredoxin-like [2Fe-2S] ferredoxin from Aquifex aeolicus.
abstract: The 2.3 A resolution crystal structure of a [2Fe-2S] cluster containing ferredoxin from Aquifex aeolicus reveals a thioredoxin-like fold that is novel among iron-sulfur proteins. The [2Fe-2S] cluster is located near the surface of the protein, at a site corresponding to that of the active-site disulfide bridge in thioredoxin. The four cysteine ligands are located near the ends of two surface loops. Two of these ligands can be substituted by non-native cysteine residues introduced throughout a stretch of the polypeptide chain that forms a protruding loop extending away from the cluster. The presence of homologs of this ferredoxin as components of more complex anaerobic and aerobic electron transfer systems indicates that this is a versatile fold for biological redox processes.
authors.affiliation: Division of Chemistry and Chemical Engineering 147-75CH, California Institute of Technology, Pasadena, CA, 91125, USA.
authors.forename: A P
authors.initials: AP
authors.lastname: Yeh
authors.forename: C
authors.initials: C
authors.lastname: Chatelet
authors.forename: S M
authors.initials: SM
authors.lastname: Soltis
authors.forename: P
authors.initials: P
authors.lastname: Kuhn
authors.forename: J
authors.initials: J
authors.lastname: Meyer
authors.forename: D C
authors.initials: DC
authors.lastname: Rees
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D001419:Bacteria / Q000737:chemistry* / Q000235:genetics; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D019281:Dimerization; D004220:Disulfides / Q000378:metabolism; D042967:Electron Transport Complex I; D005288:Ferredoxins / Q000737:chemistry* / Q000235:genetics; D007501:Iron / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008983:Molybdoferredoxin / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry; D009591:Nitrogenase / Q000378:metabolism; D011485:Protein Binding; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013455:Sulfur / Q000378:metabolism; D013879:Thioredoxins / Q000737:chemistry*
chemical_list: D004220:Disulfides; D005288:Ferredoxins; D008024:Ligands; D008983:Molybdoferredoxin; D013879:Thioredoxins; D013455:Sulfur; D007501:Iron; D009591:Nitrogenase; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I; D003545:Cysteine