High-performance liquid chromatographic assay to detect hydroxylate and conjugate metabolites of propofol in human urine.
This paper describes a HPLC method for the simultaneous detection of phase I (2,6-diisopropyl-1-4-quinol and 2,6-diisopropyl-1-4-quinone) and phase II (4-(2,6-diisopropyl-1-4-quinol)-sulphate, 1-(2,6-diisopropyl-1-4-quinol)-glucuronide, 4-(2,6-diisopropyl-1-4-quinol)-glucuronide, and propofol-glucuronide) metabolites of propofol in human urine samples. Separation was based on a simple mobile phase and a reversed-phase chromatographic column. Metabolite identification was performed by UV spectrum on a diode-array detector and by LC-APCI-MS. The identification was also carried out using in vitro incubation mixtures (cytosol and microsomes prepared from liver) from several species: human, rat and rabbit. This assay was performed using UV, fluorescence and electrochemical detection modes. Each of these was analyzed and discussed.
Favetta
P
P
Département de Pharmacie Clinique de Pharmacocinétique et d'Evaluation du Médicament, Institut des Sciences Pharmaceutiques et Biologiques de Lyon, France.
Guitton
J
J
Degoute
C S
CS
Van Daele
L
L
Boulieu
R
R
D018686:Anesthetics, Intravenous / Q000652:urine*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry; D008862:Microsomes, Liver / Q000378:metabolism; D015742:Propofol / Q000652:urine*; D011817:Rabbits; D051381:Rats; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D018686:Anesthetics, Intravenous; D015742:Propofol