Homologous mutations in two diverse sulphate transporters have similar effects.
Mutations in the human sulphate transporter gene, DTDST, have been implicated in several diseases. Analysis of affected patients has linked disease symptoms to faulty sulphate transporter activity. We have reproduced two of these mutations in SHST1, a homologous member of the family isolated from the tropical legume, Stylosanthes hamata. Both mutations significantly reduce sulphate transport activity of SHST1. These results indicate that conserved residues between distinct members of the family may share essential roles in structure or function. The results also suggest that putative helix 9 may be important for stability and/or trafficking of SHST1 to the plasma membrane.
Khurana
O K
OK
Division of Biochemistry and Molecular Biology, The Faculties, Australian National University, 0200, Canberra, A.C.T., Australia.
Coupland
L A
LA
Shelden
M C
MC
Howitt
S M
SM
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D027321:Anion Transport Proteins; D001692:Biological Transport; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D017124:Conserved Sequence / Q000235:genetics; D007887:Fabaceae; D005816:Genetic Complementation Test; D006801:Humans; D007700:Kinetics; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D010946:Plants, Medicinal; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D013329:Structure-Activity Relationship; D000076988:Sulfate Transporters; D013816:Thermodynamics
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; C089651:SLC26A2 protein, human; D000076988:Sulfate Transporters