Structural study of the exopolysaccharide produced by a clinical isolate of Burkholderia cepacia.
The primary structure of the exopolysaccharide produced by a clinical isolate of the bacterium Burkholderia cepacia was studied by means of methylation analysis, selective degradation, NMR spectroscopy, and electrospray mass spectrometry. The resulting data showed that the parent repeating unit of the exopolysaccharide is a highly branched heptasaccharide with the following structure: Two acetyl groups are present per repeating unit, as noncarbohydrate substituents.
Cescutti
P
P
Dipartimento BBCM, Università di Trieste, via L. Giorgieri 1, Trieste, I-34127, Italy. cescutti@bbcm.univ.trieste.it
Bosco
M
M
Picotti
F
F
Impallomeni
G
G
Leitão
J H
JH
Richau
J A
JA
Sá-Correia
I
I
D016956:Burkholderia cepacia / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry*
D011135:Polysaccharides, Bacterial