Enhancing effect of saccharides on the mutagenicity of 2-chloro-4-methylthiobutanoic acid.
The mutagenicity of 2-chloro-4-methylthiobutanoic acid (CMBA), a nitrite-treated Sanma fish mutagen, in Salmonella typhimurium TA100 was enhanced by addition of D-glucose during the CMBA-treatment. Several other monosaccharides also enhanced the mutagenicity of CMBA, and the order of the enhancing potency was found to be D-mannose, D-glucose>D-fructose, D-ribose, D-galactose. A disaccharide, maltose, showed only little enhancement. No enhancement was found with L-glucose. We investigated whether saccharides affect uptake of [methyl-14C]CMBA into S. typhimurium TA100. Saccharides which enhanced CMBA-induced mutagenesis increased the uptake. L-Glucose did not enhance the uptake. There was good correlation between the enhanced mutagenesis and increased radioactivity in Salmonella, suggesting that the enhancing effect of monosaccharide on the CMBA-induced mutagenesis results from the enhanced uptake of the mutagen into bacteria.
Kimura
S
S
School of Humanities for Environmental Policy and Technology, Himeji Institute of Technology, Hyogo, Japan.
Arimoto-Kobayashi
S
S
Hayatsu
H
H
D002087:Butyrates / Q000378:metabolism / Q000633:toxicity*; D002250:Carbon Radioisotopes / Q000378:metabolism; D004357:Drug Synergism; D005632:Fructose / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D008358:Mannose / Q000494:pharmacology; D009005:Monosaccharides / Q000494:pharmacology*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D012266:Ribose / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013438:Sulfhydryl Compounds; D013997:Time Factors
C093068:2-chloro-4-methylthiobutanoic acid; D002087:Butyrates; D002250:Carbon Radioisotopes; D009005:Monosaccharides; D009153:Mutagens; D013438:Sulfhydryl Compounds; D005632:Fructose; D012266:Ribose; D005947:Glucose; D008358:Mannose; D005690:Galactose