title: Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium.
abstract: Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.
authors.affiliation: The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73402, USA.
authors.forename: A T
authors.initials: AT
authors.lastname: Trieu
authors.forename: S H
authors.initials: SH
authors.lastname: Burleigh
authors.forename: I V
authors.initials: IV
authors.lastname: Kardailsky
authors.forename: I E
authors.initials: IE
authors.lastname: Maldonado-Mendoza
authors.forename: W K
authors.initials: WK
authors.lastname: Versaw
authors.forename: L A
authors.initials: LA
authors.lastname: Blaylock
authors.forename: H
authors.initials: H
authors.lastname: Shin
authors.forename: T J
authors.initials: TJ
authors.lastname: Chiou
authors.forename: H
authors.initials: H
authors.lastname: Katagi
authors.forename: G R
authors.initials: GR
authors.lastname: Dewbre
authors.forename: D
authors.initials: D
authors.lastname: Weigel
authors.forename: M J
authors.initials: MJ
authors.lastname: Harrison
mesh_terms: D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis; D018014:Gene Transfer Techniques; D000455:Medicago sativa / Q000235:genetics*; D010944:Plants / Q000235:genetics; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA