The impact of ofloxacin on rat testicular DNA: application of image analysis.
Ofloxacin induces its antibacterial action mainly by inhibition of DNA gyrase which is equivalent to topoisomerase II in mammalian cells. The present study was focused on the impact of ofloxacin on rat testicular DNA ploidy in a dose-response relationship using an image analysis technique on testicular sections following Fuelgen DNA staining. Sperm count and motility as well as sperm head abnormality tests were also investigated. Ofloxacin was given p.o. in doses of 36, 72 and 360 mg kg(-1) day(-1) for 15 consecutive days. The animals were then left to live safely to day 60 from starting the experiment to give them a chance to complete the cycle of spermatogenesis. Results revealed that ofloxacin significantly decreased the percentage of haploid cells in a dose-dependent manner with concomitant increase in the percentage of diploid cells. Sperm count and motility was also markedly decreased in a dose-dependent fashion. Sperm head abnormality showed no marked change following ofloxacin treatment. In conclusion, ofloxacin induced a marked disturbance in rat testicular DNA ploidy, which may be explained on the basis of cross-reactivity to topoisomerase II.
Abd-Allah
A R
AR
Department of Pharmacology & Toxicology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
Gannam
B B
BB
Hamada
F M
FM
D000818:Animals; D000890:Anti-Infective Agents / Q000633:toxicity*; D004396:Coloring Agents; D004247:DNA / Q000187:drug effects* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D007091:Image Processing, Computer-Assisted; D008297:Male; D015242:Ofloxacin / Q000633:toxicity*; D011003:Ploidies; D051381:Rats; D012394:Rosaniline Dyes; D013076:Sperm Count; D013077:Sperm Head / Q000187:drug effects / Q000473:pathology; D013081:Sperm Motility / Q000187:drug effects; D013082:Sperm Tail / Q000187:drug effects / Q000473:pathology; D013194:Staining and Labeling; D013737:Testis / Q000187:drug effects* / Q000473:pathology
D000890:Anti-Infective Agents; D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA; D015242:Ofloxacin