title: IL-1-mediated expression of membrane type matrix-metalloproteinase in rheumatoid osteoblasts.
abstract: OBJECTIVE
  To determine whether the activation of metalloproteinases can be achieved by the interaction of the inflammatory cytokine, IL-1 beta, with rheumatoid osteoblasts.
  
  
  RESULTS
  MMP-2 is not secreted by rheumatoid osteoblasts as a proenzyme; however, IL-1 beta stimulation induced the secretion of MMP-2 as an active form from rheumatoid osteoblasts. This MMP-2 activating activity was detected significantly in IL-1 beta-stimulated rheumatoid osteoblasts. In support of this result, IL-1 beta stimulation induced the expression of membrane type matrix-metalloproteinase (MT-MMP), a newly-identified MMP-2 specific activator, in rheumatoid osteoblasts.
  
  
  CONCLUSIONS
  These results suggest that IL-1 beta induces MMP-2 activation in part by up-regulating MT-MMP expression and represents a new mechanism for cytokine-mediated articular destruction in RA.
authors.affiliation: School of Allied Medical Sciences, Nagasaki University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Origuchi
authors.forename: K
authors.initials: K
authors.lastname: Migita
authors.forename: T
authors.initials: T
authors.lastname: Nakashima
authors.forename: M
authors.initials: M
authors.lastname: Tominaga
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Nakashima
authors.forename: T
authors.initials: T
authors.lastname: Aoyagi
authors.forename: A
authors.initials: A
authors.lastname: Kawakami
authors.forename: Y
authors.initials: Y
authors.lastname: Kawabe
authors.forename: K
authors.initials: K
authors.lastname: Eguchi
mesh_terms: D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000276:immunology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D020778:Matrix Metalloproteinase 2