title: Spinal GABA(A) receptors do not mediate the sympathetic baroreceptor reflex in the rat.
abstract: Activation of baroreceptors causes efferent sympathetic nerve activity (SNA) to fall. Two mechanisms could account for this sympathoinhibition: disfacilitation of sympathetic preganglionic neurons (SPN) and/or direct inhibition of SPN. The roles that spinal GABA and glycine receptors play in the baroreceptor reflex were examined in anesthetized, paralyzed, and artificially ventilated rats. Spinal GABA(A) receptors were blocked by an intrathecal injection of bicuculline methiodide, whereas glycine receptors were blocked with strychnine. Baroreceptors were activated by stimulation of the aortic depressor nerve (ADN), and a somatosympathetic reflex was used as control. After an intrathecal injection of vehicle, there was no effect on any measured variable or evoked reflex. In contrast, bicuculline caused a dose-dependent increase in arterial pressure, SNA, phrenic nerve discharge, and it significantly facilitated the somatosympathetic reflex. However, bicuculline did not attenuate either the depressor response or sympathoinhibition evoked after ADN stimulation. Similarly, strychnine did not affect the baroreceptor-induced depressor response. Thus GABA(A) and glycine receptors in the spinal cord have no significant role in baroreceptor-mediated sympathoinhibition.
authors.affiliation: Hypertension and Stroke Research Laboratories, Departments of Physiology and Neurosurgery, University of Sydney, Royal North Shore Hospital, St. Leonards, NSW 2065, Australia.
authors.forename: A K
authors.initials: AK
authors.lastname: Goodchild
authors.forename: B T
authors.initials: BT
authors.lastname: Van Deurzen
authors.forename: Q J
authors.initials: QJ
authors.lastname: Sun
authors.forename: J
authors.initials: J
authors.lastname: Chalmers
authors.forename: P M
authors.initials: PM
authors.lastname: Pilowsky
mesh_terms: D000320:Adrenergic Fibers / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000294:innervation; D017704:Baroreflex / Q000502:physiology*; D001640:Bicuculline / Q000008:administration & dosage / Q000031:analogs & derivatives*; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002245:Carbon Dioxide / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D058787:GABA-A Receptor Antagonists; D007275:Injections, Intravenous; D007278:Injections, Spinal; D010791:Phrenic Nerve / Q000187:drug effects / Q000502:physiology; D010812:Physical Stimulation; D011659:Pulmonary Gas Exchange; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects; D011963:Receptors, GABA-A / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D013153:Splanchnic Nerves / Q000187:drug effects / Q000502:physiology; D013331:Strychnine / Q000008:administration & dosage
chemical_list: D058787:GABA-A Receptor Antagonists; D011963:Receptors, GABA-A; D002245:Carbon Dioxide; C017069:bicuculline methiodide; D013331:Strychnine; D001640:Bicuculline