Genetic variation among European strains of Saccharomyces paradoxus: results from DNA fingerprinting.
We used microsatellite fingerprinting and RAPD analysis to characterize 28 wild European strains of Saccharomyces paradoxus. In contrast to our results from a previous allozyme survey [Naumov et al. Int. J. Syst. Bacteriol. 47: 341-344 (1997a)], these methods revealed extensive genetic variation. The RAPD primers 5'AATCGGGCTG and 5'GGGTAACGCC and the microsatellite primer (GTG)5 yielded reproducible amplification patterns of sufficient clarity and variability to distinguish individual strains from the wild. UPGMA analysis tended to group the strains according to climatic and geographic origin. A comparative study of Ty1 sequence having multiple chromosomal location was also done. Each wild S. paradoxus isolate shows a unique hybridization pattern allowing discrimination to the strain level.
Naumov
E S
ES
State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Naumov
G I
GI
Molina
E I
EI
D015139:Blotting, Southern; D016172:DNA Fingerprinting / Q000379:methods*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D005060:Europe; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique; D018626:Retroelements / Q000235:genetics; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics*
D004271:DNA, Fungal; D018626:Retroelements