Peptoid-containing collagen mimetics with cell binding activity.
Collagen mimetic peptides containing the peptoid residue Nleu (Goodman Bhumralkar, Jefferson, Kwak, Locardi. Biopolymers 1998;47:127-142) were tested for interactions with epithelial cells and fibroblasts. Molecules containing the sequence Gly-Pro-Nleu with a minimum of nine repeats showed cell binding activity. The activity of these molecules appeared to be conformationally sensitive, with the triple-helical form being preferred. When immobilized on a surface, the (Gly-Pro-Nleu)(10)-Gly-Pro-NH(2) sequence stimulated the attachment and growth of corneal epithelial cells and fibroblasts and the migration of epithelial tissue. The peptide sequence KDGEA inhibited cell attachment to the (Gly-Pro-Nleu)(10)-Gly-Pro-NH(2) sequence, suggesting that cell binding to this collagen mimetic involves the alpha2beta1 heterodimer integrin receptor. Interestingly, peptides containing the sequence (GlyNleu-Pro-)(10)-NH(2) did not have cell binding activity. The discovery that triple-helical peptides containing the Gly-Pro-Nleu sequences interact with cells opens up new opportunities in the design of collagen mimetic biomaterials.
Johnson
G
G
Cooperative Research Centre for Eye Research and Technology, P.O. Box 184, North Ryde, New South Wale 1670. graham.johnson@molsci.csiro.au
Jenkins
M
M
McLean
K M
KM
Griesser
H J
HJ
Kwak
J
J
Goodman
M
M
Steele
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D003315:Cornea / Q000166:cytology / Q000187:drug effects; D004355:Drug Stability; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D034444:Peptoids; D011489:Protein Denaturation
D001672:Biocompatible Materials; D009842:Oligopeptides; D034444:Peptoids; D003094:Collagen