title: Specific antibody promotes opsonization and PMN-mediated killing of phagocytosis-resistant Enterococcus faecium.
abstract: Many clinical isolates of Enterococcus faecium are resistant to neutrophil (PMN)-mediated phagocytosis and killing in the presence of normal human serum. We have now examined the ability of specific polyclonal rabbit antibodies to promote opsonization and killing of phagocytosis-resistant E. faecium. Immune rabbit serum generated against formalin-killed E. faecium TX0016, a phagocytosis-resistant strain, markedly promoted binding of TX0016 organisms to PMNs and PMN-mediated killing. These effects were dramatically reduced by (a) adsorption of immune serum with E. faecium TX0016, but not by adsorption with a strain of E. faecium susceptible to phagocytosis, and (b) incubation of immune serum with carbohydrate purified from TX0016, but not by incubation with a surface protein extract from TX0016. IgG purified from immune serum was unable by itself to promote bacterial binding to PMNs. However, specific IgG was able to promote binding to PMNs and PMN-mediated killing in the presence of normal human serum as a complement source, as were F(ab')(2) and Fab fragments produced from it, and the alternative pathway of complement was sufficient to promote IgG- and F(ab')(2)-mediated opsonization. PMN complement receptor type 3, but not complement receptor type 1, was involved in bacterial binding to PMNs induced by the combination of F(ab')(2) fragments and normal human serum. These results suggest that opsonization by antibodies potentially directed against bacterial carbohydrate, in conjunction with complement activation, has an important role in the host defense against phagocytosis-resistant E. faecium.
authors.affiliation: Virginia Mason Medical Center. Seattle, WA 98111, USA. cidrmr@vmmc.org
authors.forename: R M
authors.initials: RM
authors.lastname: Rakita
authors.forename: V C
authors.initials: VC
authors.lastname: Quan
authors.forename: K
authors.initials: K
authors.lastname: Jacques-Palaz
authors.forename: K V
authors.initials: KV
authors.lastname: Singh
authors.forename: R C
authors.initials: RC
authors.lastname: Arduino
authors.forename: M
authors.initials: M
authors.lastname: Mee
authors.forename: B E
authors.initials: BE
authors.lastname: Murray
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D003167:Complement Activation; D016984:Enterococcus faecium / Q000276:immunology* / Q000378:metabolism; D016908:Gram-Positive Bacterial Infections / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D007106:Immune Sera; D009895:Opsonin Proteins