title: Biliary and urinary metabolic profiles of 1,2-diethyl-3-hydroxypyridin-4-one (CP94) in the rat.
abstract: This study compares the biliary and urinary metabolic profiles of 1,2-diethyl-3-hydroxypyridin-4-one (CP94), an orally active iron chelator, in the normal rat. Surprisingly, CP94 was found to form two phase II metabolites, the 3-O- and 4-O-glucuronides. These glucuronides accounted for 38 and 28% of the administered CP94 dose, in bile and urine, respectively. Unchanged CP94 accounted for 5% of the CP94 dose in both bile and urine. The 2-(1'-hydroxy) metabolite of CP94 was found to be the dominant metabolite in urine. In addition, an unstable metabolite was detected in the bile although its structure remains unknown at the present stage. The excretion of iron in bile, after administration of CP94, was found to parallel the biliary elimination of CP94 together with its hydroxylated derivatives, indicating the importance of metabolites in iron excretion.
authors.affiliation: Department of Pharmacy, King's College London, London, United Kingdom.
authors.forename: S L
authors.initials: SL
authors.lastname: Lu
authors.forename: I
authors.initials: I
authors.lastname: Gosriwatana
authors.forename: D Y
authors.initials: DY
authors.lastname: Liu
authors.forename: Z D
authors.initials: ZD
authors.lastname: Liu
authors.forename: A I
authors.initials: AI
authors.lastname: Mallet
authors.forename: R C
authors.initials: RC
authors.lastname: Hider
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D007502:Iron Chelating Agents / Q000378:metabolism; D008297:Male; D011725:Pyridines / Q000378:metabolism; D011728:Pyridones / Q000032:analysis / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar
chemical_list: C121031:3-hydroxypyridin-4-one; D007502:Iron Chelating Agents; D011725:Pyridines; D011728:Pyridones; C068543:1,2-diethyl-3-hydroxypyridin-4-one; C108010:CP 102