Reduction of pulmonary mast cells in areas of acute inflammation in calves with Mannheimia (Pasteurella) haemolytica pneumonia.
Mast cells in the left cranial pulmonary lobe of colostrum-deprived neonatal calves were quantified 2 and 6 h after intrabronchial inoculation with Mannheimia (Pasteurella) haemolytica A1. The mast cells were detected (1) immunohistochemically with a mouse anti-human mast cell tryptase monoclonal antibody, and (2) by metachromatic staining with low pH toluidine blue. A greater number of mast cells was demonstrated by the second method than by the first. At 6 h after inoculation, but not at 2 h, the number of mast cells was significantly reduced at the site of the main lesions. Treatment of calves with a sialyl Lewis mimetic (TBC1269) did not appreciably affect the results at 6 h.
Ramírez-Romero
R
R
Department of Veterinary Pathology, Iowa State University, Ames, Iowa 50011-1250, USA.
Brogden
K A
KA
Gallup
J M
JM
Dixon
R A
RA
Ackermann
M R
MR
D000208:Acute Disease; D000818:Animals; D001713:Biphenyl Compounds / Q000627:therapeutic use; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D008168:Lung / Q000276:immunology / Q000473:pathology*; D008297:Male; D016978:Mannheimia haemolytica; D008358:Mannose / Q000031:analogs & derivatives; D008362:Mannosides / Q000627:therapeutic use; D008407:Mast Cells / Q000276:immunology / Q000473:pathology*; D012766:Pasteurellosis, Pneumonic / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*
D001713:Biphenyl Compounds; D008362:Mannosides; C108700:bimosiamose disodium; D008358:Mannose