title: The histidine decarboxylase (HDC) gene of Tetrahymena pyriformis is similar to the mammalian one. A study of HDC expression.
abstract: RNA was isolated from Tetrahymena pyriformis GL and using human histidine decarboxylase (HDC) gene primers, the RT-PCR product was sequenced. A fraction containing 207 base pairs was compared to the published sequences of prokaryotic and mammalian (rat, mouse and human) HDC cDNA (exons). The HDC-cDNA fraction of Tetrahymena was similar to the mammalian cDNA-s and it was completely different from the prokaryotic HDC-gene. The results indicate the presence of a mammalian-like HDC-gene already in a unicellular eukaryote organism and demonstrates also that the divergence of the prokaryotic-eukaryotic common gene took place already at this low evolutionary level.
authors.affiliation: Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine.
authors.forename: H
authors.initials: H
authors.lastname: Hegyesi
authors.forename: C
authors.initials: C
authors.lastname: Szalai
authors.forename: A
authors.initials: A
authors.lastname: Falus
authors.forename: G
authors.initials: G
authors.lastname: Csaba
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D006640:Histidine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008322:Mammals / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid; D013769:Tetrahymena pyriformis / Q000201:enzymology / Q000235:genetics*
chemical_list: D006640:Histidine Decarboxylase