Nitric oxide synthesis causes inositol phosphate production and Ca2+ release in rat parotid acinar cells.
The activity of nitric oxide synthase (NOS) in rat parotid acinar cells was measured using a newly synthesized fluorescent NO indicator DAF-2/DA. Our results show that NO production is most effectively stimulated by activation of the beta-adrenergic receptor, and to a minor extent by substance P (SP). NO activates the production of cGMP, an intracellular messenger that has been shown to release Ca2+ from ryanodine-sensitive intracellular stores. We found that cGMP is also able to release Ca2+ from ryanodine-insensitive intracellular stores. Our data show that a rise in the cGMP concentration induces inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] synthesis and Ca2+ release from intracellular stores.
Tritsaris
K
K
Department of Oral Function and Physiology, The Panum Institute, University of Copenhagen, Denmark.
Looms
D K
DK
Nauntofte
B
B
Dissing
S
S
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D006152:Cyclic GMP / Q000096:biosynthesis / Q000494:pharmacology; D019793:Fluorescein; D007202:Indicators and Reagents; D007295:Inositol Phosphates / Q000096:biosynthesis*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000502:physiology; D010306:Parotid Gland / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000494:pharmacology
C111205:4,5-diaminofluorescein; D000318:Adrenergic beta-Agonists; D007202:Indicators and Reagents; D007295:Inositol Phosphates; D009569:Nitric Oxide; D013373:Substance P; D006152:Cyclic GMP; D007545:Isoproterenol; D002118:Calcium; D019793:Fluorescein