title: Influence of phonation on basement membrane zone recovery after phonomicrosurgery: a canine model.
abstract: The process of wound healing at the basement membrane zone of the vocal folds after phonomicrosurgery was investigated. The influence of phonation and the implied optimal period of voice rest were considered. Phonomicrosurgery was performed on both vocal folds of 20 adult dogs. In 10 dogs, the left recurrent laryngeal nerve was simultaneously resected to simulate iatrogenic voice rest; the remaining 10 dogs were allowed to phonate normally. The healing process of the vocal folds of each group was observed at 1, 2, 4, 8, and 12 weeks after surgery with immunohistochemical staining and transmission electron microscopy. In the voice rest group, the basement membrane was completely re-formed in 2 weeks, and the "cover" appeared completely rearranged by 8 weeks. The results of the phonation group were characterized by a delayed healing process and basement membrane changes. These results suggest that voice rest of at least 2 weeks after surgery may be beneficial and that vocal hygiene should be maintained for as long as 8 weeks.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, St Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul.
authors.forename: S H
authors.initials: SH
authors.lastname: Cho
authors.forename: H T
authors.initials: HT
authors.lastname: Kim
authors.forename: I J
authors.initials: IJ
authors.lastname: Lee
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000737:chemistry / Q000648:ultrastructure; D004285:Dogs; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D007830:Larynx / Q000648:ultrastructure; D008854:Microscopy, Electron; D008866:Microsurgery; D010699:Phonation; D014827:Vocal Cords / Q000737:chemistry / Q000601:surgery* / Q000648:ultrastructure*; D014945:Wound Healing
chemical_list: D007797:Laminin