Differential immediate-early gene responses to shear stress and intraluminal pressure in intact human conduit vessels.
We have previously shown distinct effects of shear stress and pressure on transcription of several potent vascular mediators. In the present study, we tested the hypothesis that c-jun and c-fos are regulated differentially by shear and pressure. Intact human umbilical veins were perfused with various combinations of shear and pressure during 1.5, 3 and 6 h. Protein and gene expressions were assessed by immunofluorescence and real-time reverse transcription PCR, respectively. Shear stress and pressure exert differential temporal effects on c-jun and c-fos gene and protein expression, and these immediate-early gene responses appear to be cell-type specific for endothelial and smooth muscle cells.
Gan
L
L
Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Ostra, Göteborg University, SE 416 85, Göteborg, Sweden.
Doroudi
R
R
Hägg
U
U
Johansson
A
A
Selin-Sjögren
L
L
Jern
S
S
D004730:Endothelium, Vascular / Q000378:metabolism* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early / Q000235:genetics*; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D006439:Hemodynamics / Q000502:physiology; D006801:Humans; D006874:Hydrostatic Pressure; D066298:In Vitro Techniques; D009130:Muscle, Smooth / Q000378:metabolism; D009928:Organ Specificity; D010477:Perfusion; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013314:Stress, Mechanical; D013997:Time Factors; D014471:Umbilical Veins; D015854:Up-Regulation
D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger