title: A (17)O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum.
abstract: The (17)O NMR of bromoperoxidase in Tris buffer at pH 8 treated with (17)O-enriched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO2(O2)2(2-) is detected. The results are counter-checked with NMR data on peroxovanadium model compounds.
authors.affiliation: Institut für Anorganische und Angewandte Chemie, Universität Hamburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Casný
authors.forename: D
authors.initials: D
authors.lastname: Rehder
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Vilter
authors.forename: V
authors.initials: V
authors.lastname: Conte
mesh_terms: D001665:Binding Sites; D009682:Magnetic Resonance Spectroscopy; D010103:Oxygen Isotopes / Q000032:analysis*; D010544:Peroxidases / Q000378:metabolism*; D010545:Peroxides / Q000378:metabolism*; D000459:Phaeophyceae / Q000201:enzymology*; D014639:Vanadium / Q000378:metabolism*; D014673:Vecuronium Bromide / Q000378:metabolism
chemical_list: D010103:Oxygen Isotopes; D010545:Peroxides; D014639:Vanadium; D014673:Vecuronium Bromide; D010544:Peroxidases; C026195:bromide peroxidase