title: Beta2-agonist extraction procedures for chromatographic analysis.
abstract: Normally, different procedures were necessary to prepare sample matrices for chromatographic determination of beta2-agonists. The present review includes sampling, pre-treatment and extraction/purification for urine, plasma, liver, meat, feeds, hair and milk powder, as previous steps for chromatographic analysis of beta2-agonists. Six methodologies were especially revised for extraction/purification namely, liquid-liquid extraction, solid-phase extraction (SPE), matrix solid-phase dispersion, immunoaffinity chromatography, dialysis and supercritical fluid extraction. SPE was discussed in detail and five mechanisms were described: adsorption, apolar, polar, ion-exchange and mixed phase. A brief conclusion in this field was also outlined.
authors.affiliation: Laboratório de Bromatologia, Nutrição e Hidrologia, Faculdade de Farmácia, Universidade de Coimbra, Portugal. fjramos@ci.uc.pt
authors.forename: F J
authors.initials: FJ
authors.lastname: dos Ramos
mesh_terms: D000318:Adrenergic beta-Agonists / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*
chemical_list: D000318:Adrenergic beta-Agonists