title: A new modular chemiluminescence immunoassay analyser evaluated.
abstract: Thyrotropin (TSH), free thyroxine (fT4) and testosterone assays have been used as a probe to evaluate the performances of a new modular chemiluminescence (CL) immunoassay analyser, the Abbott Architect 2000. The evaluation was run in parallel on other systems that use CL as the detection reaction: DPC Immulite, Chiron Diagnostics ACS-180 and ACS Centaur (TSH functional sensitivity only). TSH functional sensitivity was 0.0012, 0.009, 0.033 and 0.039 mU/I for the Architect, Immulite, ACS Centaur and ACS-180, respectively. Testosterone functional sensitivity was 0.38, 3.7 and 2.0 nmol/l for Architect, Immulite and ACS-180, respectively. Good correlation was obtained between the ACS-180 and Architect for all assays. The Immulite correlation did not agree well with the Architect or ACS-180 for fT4 and testosterone but was in good agreement for TSH. Regarding fT4 and testosterone, equilibrium dialysis and isotopic dilution gas-chromatography mass-spectrometry (GC-MS) respectively were used as reference methods. For both within- and between-run precision, the Architect showed the best reproducibility for all three analytes (CV < 6%).
authors.affiliation: Laboratory of Endocrinology and Toxicology, Azienda Ospedaliera Careggi, Firenze, Italy. ago@flownet.it
authors.forename: A
authors.initials: A
authors.lastname: Ognibene
authors.forename: C J
authors.initials: CJ
authors.lastname: Drake
authors.forename: K Y
authors.initials: KY
authors.lastname: Jeng
authors.forename: T E
authors.initials: TE
authors.lastname: Pascucci
authors.forename: S
authors.initials: S
authors.lastname: Hsu
authors.forename: F
authors.initials: F
authors.lastname: Luceri
authors.forename: G
authors.initials: G
authors.lastname: Messeri
mesh_terms: D001331:Automation; D002138:Calibration; D016022:Case-Control Studies; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood; D007037:Hypothyroidism / Q000097:blood; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007676:Kidney Failure, Chronic / Q000097:blood; D008163:Luminescent Measurements; D008297:Male; D011247:Pregnancy; D015203:Reproducibility of Results; D012217:Rheumatoid Factor / Q000097:blood; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000097:blood; D013972:Thyrotropin / Q000097:blood; D013974:Thyroxine / Q000097:blood; D013997:Time Factors
chemical_list: D013739:Testosterone; D013972:Thyrotropin; D012217:Rheumatoid Factor; D013974:Thyroxine