title: Cre-loxP-mediated bax gene activation reduces growth rate and increases sensitivity to chemotherapeutic agents in human gastric cancer cells.
abstract: Dysregulation of apoptosis may be closely related to the development of cancer and its chemoresistance. Overexpression of Bax, an inducer of apoptosis, has led to increased cell death in a variety of cancer cell lines. In this study, we investigated the effect of Bax overexpression in two gastric cancer cell lines, MKN-28 and MKN-45, using a Cre-loxP-mediated inducible expression system. After induction of bax, both cell lines showed decreased proliferation, partially due to increased cell death. Furthermore, Bax-expressing MKN-28 cells were more sensitive to cisplatin. These results indicate that up-regulation of the bax gene may provide a novel strategy for the treatment of gastric cancer.
authors.affiliation: Department of Internal Medicine (III), Tohoku University School of Medicine, Sendai, Japan. kkomatsu@doc.med.akita-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Komatsu
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Shimosegawa
authors.forename: J I
authors.initials: JI
authors.lastname: Miyazaki
authors.forename: T
authors.initials: T
authors.lastname: Toyota
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D015152:Blotting, Northern; D002945:Cisplatin / Q000494:pharmacology*; D003131:Combined Modality Therapy; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D019426:Integrases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D015533:Transcriptional Activation; D014162:Transfection; D014407:Tumor Cells, Cultured; D014764:Viral Proteins; D051028:bcl-2-Associated X Protein
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; D017931:DNA Primers; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014764:Viral Proteins; D051028:bcl-2-Associated X Protein; C045073:Cre recombinase; D019426:Integrases; D002945:Cisplatin