title: Preparation of an imidazole-conjugated oligonucleotide.
abstract: Postsynthetic modification of an oligonucleotide with an imidazole functional group was achieved by formation of an amide bond between the functional group and a single 2'-amino-2'-deoxyuridine, d-aU, of the oligonucleotide. The succinimidyl ester of N-glutaryl-histamine was synthesized under anhydrous conditions and added to the oligonucleotide in an acetonitrile-containing buffer at pH 8.0. Formation of the conjugate was assayed by digestion with snake venom phosphodiesterase and bacterial alkaline phosphatase, followed by reversed-phase HPLC to resolve constituent nucleosides. The disappearance of a peak corresponding to d-aU and the appearance of a peak that coelutes with authentic 2'-(N-glutaryl-N'-histaminyl)-2'-deoxyuridine confirmed the formation of the conjugate. Imidazole-conjugated oligonucleotides may have utility as antisense agents capable of hydrolyzing RNA.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Public Health Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA.
authors.forename: M
authors.initials: M
authors.lastname: Beban
authors.forename: P S
authors.initials: PS
authors.lastname: Miller
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D007093:Imidazoles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D009841:Oligonucleotides