Detection of human alpha-defensin-1, an antimicrobial peptide, in the fluid of jaw cysts.
OBJECTIVE
The purpose of this study was to isolate and to identify an antimicrobial peptide, human alpha-defensin-1 (HNP-1) in jaw cyst fluid.


METHODS
We collected jaw cyst fluid from 22 patients with various jaw cysts and analyzed the peptide components of them by reversed-phase high-performance liquid chromatography (HPLC). HNP-1 in the jaw cyst fluid was identified by the amino acid sequence and the molecular weight. The concentration of HNP-1 in the fluid of various jaw cysts was quantified by HPLC.


RESULTS
HNP-1 in the jaw cyst fluid was isolated, purified, and identified. The concentrations of HNP-1 in the jaw cyst fluid ranged from 27.0 to 3725.4 microg/mL.


CONCLUSIONS
HNP-1 was detected in the fluid of various types of jaw cysts.
Mizukawa
N
N
Department of Oral and Maxillofacial Surgery 1, Okayama University Dental School, Japan.
Sugiyama
K
K
Ueno
T
T
Mishima
K
K
Takagi
S
S
Sugahara
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002648:Child; D002851:Chromatography, High Pressure Liquid; D020420:Cyst Fluid / Q000737:chemistry*; D023082:Defensins; D006801:Humans; D007570:Jaw Cysts / Q000737:chemistry*; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009504:Neutrophils / Q000737:chemistry; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D023084:alpha-Defensins
D000890:Anti-Infective Agents; D023082:Defensins; D011506:Proteins; D023084:alpha-Defensins; C406413:human neutrophil peptide 1