title: Assay for Laccase activity by microcalorimetry: laccase was extracted from china lacquer of Rhus vernicifera.
abstract: The reactions between Laccase (extracted from China lacquer of Rhus vernicifera) and various substrates (3,4-Dihydroxybenzaldehyde, Guaiacol, Pyrogallol, Gallic acid) have been studied using LKB-2107 batch microcalorimetry system. Based on calorimetry, a new method has been proposed. Laccase activity and the Michaelis constant K(m) have been determined simultaneously by this method. The method is simple, sample-saving, and valid for a wider range of substrate concentrations. Furthermore, it can be extended for assaying other enzymes catalyzing reactions using this method.
authors.affiliation: Department of Chemistry, Central China Normal University, 430079, Wuhan, PR China. tzwang@mnr.whcnc.ac.cn
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: H W
authors.initials: HW
authors.lastname: Wan
authors.forename: P J
authors.initials: PJ
authors.lastname: Zhou
authors.forename: S S
authors.initials: SS
authors.lastname: Qu
mesh_terms: D002151:Calorimetry / Q000379:methods*; D007700:Kinetics; D042845:Laccase; D010088:Oxidoreductases / Q000378:metabolism*; D010947:Plants, Toxic; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors; D012251:Toxicodendron / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases; D042845:Laccase