A PCR product derived from female DNA with regional localization on the Y chromosome.
A 154-bp PCR product amplified from human female DNA mapped onto the Y chromosome under high-stringency in situ hybridization conditions. The female DNA sequence revealed an 89% homology with the HSDYZ1 sequence. When the same primers were used to amplify male DNA, a 154-bp DNA fragment was also obtained, showing a 98% homology with HSDYZ1. However, although the HSDYZ1 sequence is widely distributed along the long arm of the Y chromosome, both of these particular PCR products are di-regionally localized within this distal block of constitutive heterochromatin. In situ hybridization under lower stringency showed that these 154-bp sequences map both onto the autosomes and the Y chromosome. Overall, this paper shows (i) a new class of DNA sequences shared by the autosomes and the Y chromosome; and (ii) a substructured organization of some DNA repeats within the DYZ1 family that forms a large part of the constitutive heterochromatin of the Y chromosome.
de la Torre
J
J
Departamento de Biología, Universidad Autónoma de Madrid, Spain. joaquina@uam.es
Martínez-Ramírez
A
A
Fernández
J L
JL
Díez-Martín
J L
JL
Gómez-Pineda
A
A
Gosálvez
J
J
López-Fernández
C
C
D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000235:genetics*; D017931:DNA Primers; D005260:Female; D006570:Heterochromatin / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016133:Polymerase Chain Reaction; D014998:Y Chromosome / Q000235:genetics*
D017931:DNA Primers; D006570:Heterochromatin