Gene expression and peptide localization for LH/hCG receptor in porcine small and large luteal cells: possible regulation by opioid peptides.
The aim of the present studies was to investigate (1) the presence of LH receptor (LHR) in porcine separated small (SLCs) and large (LLCs) luteal cells taken from mid-luteal corpora lutea and (2) the influence of opioid agonist, FK 33-824 (FK) on LHR gene expression in these cells. Immunocytochemistry revealed intense staining for LHR in both SLCs and LLCs. Reverse transcription-polymerase chain reaction (RT-PCR) and Southern hybridization were used to check the effect of FK and hCG on LHR gene expression. The LHR gene expression was observed in non-stimulated LLCs and in both types of cells after treatment with FK or hCG. FK stimulated LHR gene expression in SLCs and inhibited the gene expression in LLCs. Moreover, FK inhibited and potentiated stimulatory influence of hCG on the gene expression in LLCs and SLCs, respectively. These results suggest that LHR gene expression in porcine luteal cells can be regulated by opioid peptides.
Kamiński
T
T
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, Poland.
Gawrońska
B
B
Derecka
K
K
Okrasa
S
S
Przała
J
J
D000818:Animals; D048429:Cell Size; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin / Q000494:pharmacology; D004723:Endorphins / Q000037:antagonists & inhibitors / Q000502:physiology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D011974:Receptors, LH / Q000235:genetics* / Q000378:metabolism*; D013552:Swine; D014018:Tissue Distribution
D006063:Chorionic Gonadotropin; D004723:Endorphins; D011974:Receptors, LH; D005405:D-Ala(2),MePhe(4),Met(0)-ol-enkephalin