title: Effects of fluid management on edema volume and midline shift in a rat model of ischemic stroke.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effects of fluid management on brain water content (BW) and midline shift (MLS) after a focal cerebral ischemic insult.
  
  
  METHODS
  A suture model was used to induce focal cerebral ischemia for 90 minutes (n=44). The rats were randomly assigned to 3 groups 2. 5 hours after reperfusion: dehydration (n=24), control (n=8), or hydration (n=12). BW was obtained with the wet-dry weight method 24 hours after middle cerebral artery (MCA) occlusion. In addition, MRI were obtained (n=31) 24 hours after the onset of ischemia so that the ratio of hemispheric volumes ipsilateral (IH) and contralateral (CH) to the infarct and the extent of MLS could be obtained.
  
  
  RESULTS
  Across the range from moderate dehydration to intravascular volume expansion with isotonic saline, BW of the IH increased linearly as a function of change in body weight (r(2)=0.89), whereas few changes in relation to body weight were observed in CH, indicating a preferential effect of fluid management on the infarcted hemisphere. Furthermore, the hemispheric volume ratio (IH/CH) and MLS also increased in relation to changes in body weight. However, paradoxical increases in BW, IH/CH, and extent of MLS were observed in comparison with controls when severe dehydration was produced with high-dose mannitol.
  
  
  CONCLUSIONS
  Changes in ischemic BW by fluid management correlated closely with changes in body weight except when high-dose mannitol was used. Mannitol, as a dehydrating agent, may be associated with bimodal effects, with a high dose aggravating ischemic BW.
authors.affiliation: Midwest Neurology Group, Evensville, MO, USA.
authors.forename: R P
authors.initials: RP
authors.lastname: Paczynski
authors.forename: R
authors.initials: R
authors.lastname: Venkatesan
authors.forename: M N
authors.initials: MN
authors.lastname: Diringer
authors.forename: Y Y
authors.initials: YY
authors.lastname: He
authors.forename: C Y
authors.initials: CY
authors.lastname: Hsu
authors.forename: W
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D001835:Body Weight; D001929:Brain Edema / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D002545:Brain Ischemia / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003681:Dehydration; D004195:Disease Models, Animal; D004232:Diuretics / Q000494:pharmacology; D004234:Diuretics, Osmotic / Q000494:pharmacology; D005440:Fluid Therapy; D005665:Furosemide / Q000494:pharmacology; D007552:Isotonic Solutions / Q000494:pharmacology; D008279:Magnetic Resonance Imaging; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D051381:Rats; D020318:Rats, Long-Evans; D012965:Sodium Chloride / Q000494:pharmacology; D020521:Stroke / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D014867:Water / Q000378:metabolism
chemical_list: D004232:Diuretics; D004234:Diuretics, Osmotic; D007552:Isotonic Solutions; D014867:Water; D008353:Mannitol; D012965:Sodium Chloride; D005665:Furosemide