title: Collagen type I-coating of Ti6Al4V promotes adhesion of osteoblasts.
abstract: The initial contact of osteoblasts with implant surfaces is an important event for osseointegration of implants. Osseointegration of Ti6Al4V may be improved by precoating of its surface with collagen type I. In this study, the adhesion of rat calvarial osteoblasts to uncoated and collagen type I-coated titanium alloy was investigated over a period of 24 h. Collagen type I-coating accelerates initial adhesion of osteoblasts in the presence of fetal calf serum. One hour after plating, no differences in the percentage of adherent cells between the surfaces investigated were found. Adhesion of osteoblasts to uncoated surfaces was reduced by the GRGDSP peptide by about 70%, whereas adhesion to collagen type I-coated surfaces remained unaffected by treatment of the cells with the peptide. Cell adhesion to coated materials was reduced by about 80% by anti-integrin beta1 antibody. The integrin beta1 antibody did not influence the adhesion to uncoated titanium alloy. The results suggest that osteoblasts adhere to collagen type I-coated materials via integrin beta1 but not by interacting with RGD peptides, whereas adhesion to uncoated titanium alloy is mediated by RGD sequences but not via integrin beta1. Fibronectin does not seem to be involved in the adhesion of osteoblasts to either coated or uncoated titanium alloy.
authors.affiliation: Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Dresden University of Technology, Karl-Marx-Strasse 3, 01109 Dresden, Germany. ugeissle@rcs.urz.tu-dresden.de
authors.forename: U
authors.initials: U
authors.lastname: Geissler
authors.forename: U
authors.initials: U
authors.lastname: Hempel
authors.forename: C
authors.initials: C
authors.lastname: Wolf
authors.forename: D
authors.initials: D
authors.lastname: Scharnweber
authors.forename: H
authors.initials: H
authors.lastname: Worch
authors.forename: K
authors.initials: K
authors.lastname: Wenzel
mesh_terms: D000327:Adsorption; D000497:Alloys; D000818:Animals; D001665:Binding Sites; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000648:ultrastructure; D005353:Fibronectins; D019012:Integrin beta1 / Q000378:metabolism; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D013499:Surface Properties; D014025:Titanium
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D005353:Fibronectins; D019012:Integrin beta1; D009842:Oligopeptides; C031462:titanium alloy (TiAl6V4); C047981:arginyl-glycyl-aspartic acid; D003094:Collagen; D014025:Titanium