title: An ORF from Bacillus licheniformis encodes a putative DNA repressor.
abstract: The complete sequence of a reading frame adjacent to the endo-beta-1,3-1,4-D-glucanase gene from Bacillus licheniformis is reported. It encodes a putative 171 amino acid residues protein with either, low significant sequence similarity in data banks or the corresponding orthologue in the recently sequenced Bacillus subtilis genome. Computer analyses predict a canonical Helix-Turn-Helix motif characteristic of bacterial repressors/DNA binding proteins. A maxicells assay shows that the encoded polypeptide is expressed. A DNA-protein binding, assay performed by gel electrophoresis shows that the expressed protein specifically binds to Bacillus licheniformis DNA.
authors.affiliation: Institut de Biologia Fonamental and Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Naval
authors.forename: D
authors.initials: D
authors.lastname: Aguilar
authors.forename: X
authors.initials: X
authors.lastname: Serra
authors.forename: J A
authors.initials: JA
authors.lastname: Pérez-Pons
authors.forename: J
authors.initials: J
authors.lastname: Piñol
authors.forename: J
authors.initials: J
authors.lastname: Lloberas
authors.forename: E
authors.initials: E
authors.lastname: Querol
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D019077:Helix-Turn-Helix Motifs; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012097:Repressor Proteins