Use of phosphonic acid as a generic hapten in the production of broad specificity anti-organophosphate pesticide antibody.
Phosphonic acid (trans-4-phosphono-2-butenic acid; TPB) was used as a generic hapten in order to generate broad specificity antibodies against a group of organophosphorus pesticides. The polyclonal antiserum showed, in an indirect enzyme-linked immunosorbent assay (ELISA) format, preferential binding toward pesticides containing unsaturated diethyl-phosphate functionalities rather than the equivalent thiophosphate or dimethyl structures. The level of detection in the ELISA using a heterologous system was investigated and showed a 20-fold improvement when a conjugate for which the antibody had lower affinity was immobilized on the plate. Biosensor assays using parathion as a standard indicated that the antibody had a relatively high dissociation rate, and reproducible cycles of regeneration were achieved. The potential for using TPB as a generic hapten is discussed.
Alcocer
M J
MJ
Institute of Food Research, Norwich Research Park, Colney, UK.
Dillon
P P
PP
Manning
B M
BM
Doyen
C
C
Lee
H A
HA
Daly
S J
SJ
O'Kennedy
R
R
Morgan
M R
MR
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006241:Haptens; D007306:Insecticides / Q000032:analysis* / Q000276:immunology*; D008297:Male; D063065:Organophosphonates / Q000276:immunology*; D011817:Rabbits; D012680:Sensitivity and Specificity
D000906:Antibodies; D006241:Haptens; D007306:Insecticides; D063065:Organophosphonates