title: Transmissible gastroenteritis virus induces apoptosis in swine testicular cell lines but not in intestinal enterocytes.
abstract: Evidence of apoptosis caused by infection with the Purdue strain of transmissible gastroenteritis virus (TGEV) was sought in vitro (in infected swine testicular [ST] cells) and in vivo (in the intestinal tissues of infected piglets). The methods used were (1) DNA electrophoresis for detection of DNA fragmentation, and (2) terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescein nick and labelling (TUNEL). DNA "laddering" was detected in TGEV-infected ST cells only. Numerous signs of apoptosis were detected in TGEV-infected ST cells by TUNEL assay, the positive (dark brown) staining reaction being present in the majority of cell nuclei, without background staining. No such staining was seen in TGEV-infected enterocytes at various times after inoculation of piglets. Thus, it would appear that apoptosis does not occur in the enterocytes of piglets infected with TGEV.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Suwon 441-744, Kyounggi-Do, Republic of Korea.
authors.forename: B
authors.initials: B
authors.lastname: Kim
authors.forename: O
authors.initials: O
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Tai
authors.forename: C
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation; D020895:Enterocytes / Q000473:pathology* / Q000821:virology; D020287:In Situ Nick-End Labeling; D008297:Male; D013552:Swine; D013737:Testis / Q000473:pathology* / Q000821:virology; D005760:Transmissible gastroenteritis virus / Q000502:physiology*
chemical_list: D004247:DNA