Molecular and biologic characterization of Leishmania parasites implicated in an epidemic outbreak in northwestern Argentina.
Leishmania (Viannia) braziliensis and its variants were implicated in the epidemic outbreak of mucocutaneous leishmaniasis that occurred in Salta, northwestern Argentina, in 1985. A total of 24 suspected, untreated cases were evaluated clinically and parasitologically. Four of five stable isolates were consistent with the reference strain of L. (V.) braziliensis as determined by monoclonal antibodies and indirect immunofluorescence or radioimmunobinding assays. Zymodeme analysis in agarose gels showed a close relationship with L. (V.) guyanensis and L. (V.) panamensis. All zymograms obtained with polyacrylamide gels belonged to the subgenus Viannia; the patterns were different from, but very closely related to, the reference strains of L. (V.) braziliensis as determined by dendrogram analysis. Hamsters infected with two isolates showed a pattern consistent with L. (V.) braziliensis. The pattern of development in the gut of Lutzomyia longipalpis was consistent with members of Viannia.
Segura
E L
EL
Administración Nacional de Laboratorios e Institutos de Salud, Dr. Carlos G. Malbrán, Buenos Aires, Argentina. esegura@anlis.gov.ar
Juan
N
N
Piquin
A L
AL
Cuba Cuba
C A
CA
Abramo Orrego
L
L
McMahon-Pratt
D
D
Montamat
E E
EE
Momen
H
H
Grimaldi
G
G
D000328:Adult; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D006224:Cricetinae; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007891:Leishmania / Q000145:classification*; D007892:Leishmania braziliensis / Q000302:isolation & purification*; D007897:Leishmaniasis, Mucocutaneous / Q000453:epidemiology* / Q000469:parasitology*; D008297:Male; D008647:Mesocricetus; D011863:Radioimmunoassay