Sensitive method for the determination of ambroxol in body fluids by capillary electrophoresis and fluorescence detection.
A sensitive and rapid capillary electrophoretic method combined with laser-induced fluorescence detection has been developed for the determination of ambroxol. Samples were derivatized with 5 x 10(-4) M fluorescein isothiocyanate. A linear relationship between concentration and peak area was obtained in the concentration range 0.008-42 microg ml(-1) with a correlation coefficient of 0.9999. The applicability of the method to serum and urine samples was demonstrated. The method is also useful for the determination of ambroxol in pharmaceutical preparations.
Perez-Ruiz
T
T
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain. tpr@fcu.um.es
Martínez-Lozano
C
C
Sanz
A
A
Bravo
E
E
D000551:Ambroxol / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005100:Expectorants / Q000493:pharmacokinetics*; D006801:Humans; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005100:Expectorants; D000551:Ambroxol