Analysis of genetically modified plant gene expression using GUS fluorimetry.
A fluorimetric assay method for the analysis of beta-glucuronidase (GUS) reporter gene expression in genetically modified plants is described. Optimization of this method for woody plants and a statistical approach suitable for comparisons of gene expression in different transformants or tissues of the same plant is described. Example data from elm (Ulmus procera) SR4 regenerant plants, shown to be genetically modified by PCR and DNA-DNA hybridizations, in which higher GUS expression levels are found in stems than in leaves demonstrates the utility of this approach.
Gartland
K M
KM
Plant Biotechnology Research Group, University of Abertay Dundee, Scotland.
McHugh
A T
AT
Vitha
S
S
Benes
K
K
Irvine
R J
RJ
Gartland
J S
JS
D018744:DNA, Plant / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D005470:Fluorometry / Q000379:methods*; D015870:Gene Expression; D005798:Genes, Bacterial; D017343:Genes, Plant; D017930:Genes, Reporter / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012015:Reference Standards
D018744:DNA, Plant; D005966:Glucuronidase