title: Effects of organic acids on lipid synthesis and ecdysis in Triatoma infestans eggs.
abstract: Medium chain fatty acids are known inhibitors of the activity of lipogenic enzymes at the transcriptional level. Mature Triatoma infestans eggs incorporate [14C]-acetate into phospholipid (PL), triacylglycerol (TG), and free fatty acid (FFA) fractions. In the presence of sodium octanoate (SO) or sodium phenylethylpropionate (PHEP), lipid synthesis is inhibited in a dose-dependent manner. When eggs are incubated in vivo with [14C]-acetate, the usual fatty acid products are largely palmitic and oleic acids. However, in the presence of SO (5-10 mM), the elongation of [14C]-acetate units is interrupted at [14C] eight total carbons. Eggs incubated in vivo with [14C]-SO, accumulate most of the label in the FFA fraction. SO (> 0.1 mM) but not sodium hexanoate (SH), inhibits the activity of microsomal and cytosolic fatty acid synthetases (FAS's), measured as [14C]-malonyl-CoA incorporation. PHEP (1 mM) and SO (10 mM) also produce major alterations in egg hatching and survival of the emerged insects, after a 1 h immersion bioassay.
authors.affiliation: Instituto de Investigaciones Bioquímicas de la Plata, Facultad de Ciencias Médicas, UNLP, La Plata, Argentina. mjuarez@isis.unlp.edu.ar
authors.forename: M P
authors.initials: MP
authors.lastname: Juárez
authors.forename: R
authors.initials: R
authors.lastname: Napolitano
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D002208:Caproates / Q000494:pharmacology; D002210:Caprylates / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D010063:Ovum / Q000187:drug effects / Q000378:metabolism*; D011422:Propionates / Q000494:pharmacology; D012098:Reproduction / Q000187:drug effects; D014224:Triatoma / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*
chemical_list: D000085:Acetates; D002208:Caproates; D002210:Caprylates; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D008055:Lipids; D011422:Propionates; C037652:hexanoic acid; D064429:Fatty Acid Synthases; C031492:octanoic acid