One- and two-dimensional gel electrophoresic identification of African yam bean seed proteins.
Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose-Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. N-terminal sequencing of prominently resolved polypetide bands led to identification of proteins having sequence homology with characterized legume seed proteins, namely, mung bean seed albumin, pea alpha-fucosidase, soybean Kunitz-type trypsin inhibitor, an endochitinase, pea pathogenesis-related protein, and/or cowpea seed storage proteins. Minor lectin-like proteins lacking hemagglutinating activity against rabbit and human erythrocytes were also identified. Because proteins such as protease inhibitors, chitinases, pathogenesis-related proteins, and lectins are known to have antimetabolic effects, the findings from this study may have relevance in the acceptability, adoption, and utilization of AYB as human food.
Machuka
J
J
International Institute of Tropical Agriculture, C/O LW Lambourn & Company Ltd., Croydon, United Kingdom. j.machuka@cgiar.org
Okeola
O G
OG
D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007887:Fabaceae / Q000737:chemistry*; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D008969:Molecular Sequence Data; D009753:Nutritive Value; D010446:Peptide Fragments / Q000737:chemistry; D037121:Plant Lectins; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal; D011817:Rabbits; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D037102:Lectins; D010446:Peptide Fragments; D037121:Plant Lectins; D010940:Plant Proteins