A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.
Only a few human malignant peripheral nerve sheath tumour (MPNST)-cell lines have been reported, and their characteristics have not been fully established. In this study, we established a new human cell line, HS-Sch-2, from an MPNST of the ordinary type which arose in a 54-year-old woman without von Recklinghausen's disease. This cell line was characterized by chromosome analysis, immunohistochemistry, ultrastructural examination, and direct sequencing of the p53 gene. The HS-Sch-2 cells have grown for more than 48 months in vitro, and exhibited hypotriploid karyotypes with complex chromosome abnormalities lacking a specific pattern. Histological features of the heterotranplanted nude mouse tumours were essentially the same as those of the original MPNST, with positive reactions for S-100 protein and neuron-specific enolase but not for epithelial membrane antigen, fibronectin or CD34. Ultrastructural examination in vivo revealed intricate interdigitation of long cytoplasmic processes and basal lamina-like structures. In addition, direct sequencing of the p53 gene detected a point mutation from CGT to CAT at codon 273 in exon 8. This HS-Sch-2 cell line, which exhibits distinctive morphological characteristics of MPNST and a p53 point mutation, will be useful for biological and pathological investigations of MPNST.
Sonobe
H
H
Department of Pathology, Kochi Medical School, Kohasu, Okoh, Nankoku, Kochi 783-8505, Japan.
Takeuchi
T
T
Furihata
M
M
Taguchi
T
T
Kawai
A
A
Ohjimi
Y
Y
Iwasaki
H
H
Kaneko
Y
Y
Ohtsuki
Y
Y
D000818:Animals; D002415:Cats; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D018317:Nerve Sheath Neoplasms / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D010524:Peripheral Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D017354:Point Mutation / Q000235:genetics*; D012583:Schwann Cells / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000648:ultrastructure