title: A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model.
abstract: Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.
authors.affiliation: Department of Pathology, University of Sydney, Camperdown, NSW, Australia. pmac@zip.com.au
authors.forename: C S
authors.initials: CS
authors.lastname: McLachlan
authors.forename: J L
authors.initials: JL
authors.lastname: Yin
authors.forename: C
authors.initials: C
authors.lastname: Driussi
authors.forename: P R
authors.initials: PR
authors.lastname: Jusuf
authors.forename: B
authors.initials: B
authors.lastname: Hambly
authors.forename: M A
authors.initials: MA
authors.lastname: McGuire
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D006333:Heart Failure / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits