High-dose mouse immunoglobulin G administration suppresses Theiler's murine encephalomyelitis virus-induced demyelinating disease.
We studied the effect of high-dose mouse IgG on TMEV-induced demyelinating disease (TMEV-IDD). We injected TMEV intracerebrally into susceptible SJL/J mice and induced TMEV-IDD. Mouse IgG were injected intraperitonealy, and clinical course and various immunological indicators were studied. The results show that TMEV-IDD was significantly suppressed both clinically and histologically (P<0.01) when IgG were administered in the effector phase. The delayed type hypersensitivity and T cell proliferative response specific for TMEV were decreased by this treatment. In an ELISPOT assay, the number of TNF-alpha producing lymphocytes in the spinal cords was low in high-dose IgG treated mice compared with PBS treated control mice. These data suggest that administration of IgG suppresses TMEV-IDD and may be promising treatment to prevent exacerbation of human multiple sclerosis.
Koh
C
C
Department of Medicine (Neurology), Shinshu University School of Medicine, 3-1-1 Asahi, 390-8621, Matsumoto, Japan. kshosei@hsp.md.shinshu-u.ac.jp
Inoue
A
A
Yamazaki
M
M
Kim
B S
BS
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D018188:Cardiovirus Infections / Q000276:immunology / Q000503:physiopathology / Q000628:therapy / Q000821:virology; D003711:Demyelinating Diseases / Q000276:immunology* / Q000628:therapy* / Q000821:virology; D004195:Disease Models, Animal; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007074:Immunoglobulin G / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009103:Multiple Sclerosis / Q000276:immunology / Q000628:therapy; D013116:Spinal Cord / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D017945:Theilovirus / Q000187:drug effects* / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma