title: Hsl7p, the yeast homologue of human JBP1, is a protein methyltransferase.
abstract: The yeast protein Hsl7p is a homologue of Janus kinase binding protein 1, JBP1, a newly characterized protein methyltransferase. In this report, Hsl7p also is shown to be a methyltransferase. It can be crosslinked to [(3)H]S-adenosylmethionine and exhibits in vitro protein methylation activity. Calf histones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not. We demonstrated that JBP1 can complement Saccharomyces cerevisiae with a disrupted HSL7 gene as judged by a reduction of the elongated bud phenotype, and a point mutation in the JBP1 S-adenosylmethionine consensus binding sequence eliminated all complementation by JBP1. Therefore, we conclude the yeast protein Hsl7p is a sequence and functional homologue of JBP1. These data provide evidence for an intricate link between protein methylation and macroscopic changes in yeast morphology.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854-5635, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: J R
authors.initials: JR
authors.lastname: Cook
authors.forename: B P
authors.initials: BP
authors.lastname: Pollack
authors.forename: T G
authors.initials: TG
authors.lastname: Kinzy
authors.forename: D
authors.initials: D
authors.lastname: Norris
authors.forename: S
authors.initials: S
authors.lastname: Pestka
mesh_terms: D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005816:Genetic Complementation Test; D006657:Histones / Q000378:metabolism; D006801:Humans; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism; D016296:Mutagenesis; D004676:Myelin Basic Protein / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D017354:Point Mutation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011484:Protein-Arginine N-Methyltransferases; D012436:S-Adenosylmethionine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D014466:Ultraviolet Rays
chemical_list: D003574:Cytochrome c Group; D006657:Histones; D004676:Myelin Basic Protein; D029701:Saccharomyces cerevisiae Proteins; D012436:S-Adenosylmethionine; D008780:Methyltransferases; D011496:Protein Methyltransferases; C400529:PRMT5 protein, human; D011484:Protein-Arginine N-Methyltransferases; C120666:HSL7 protein, S cerevisiae; D011494:Protein Kinases; D005690:Galactose