title: Amino acid sequence of a nuclease (nuclease Le1) from Lentinus edodes.
abstract: The fruit bodies of Lentinus edodes produce two acid nucleases, nucleases Le1 and Le3, both of which are thought to be candidates for the enzymes producing a tasty substance, 5'-GMP. To obtain the basic information on the mechanism of production of 5'-GMP, and structure-function relationship of these nucleases, the primary structure of nuclease Le1 was estimated by both protein chemistry and gene cloning. Nuclease Le1 is a glycoprotein and consists of 290 amino acid residues, and about 2 and 6 residues of hexosamine and neutral sugar, respectively. The nucleotide sequence of cDNA and genomic DNA encoding nuclease Le1 indicated the presence of 20 amino acid residues of a signal peptide. Nuclease Le1 has 115 and 108 residues of identical amino acid residues with nucleases P1 and S, respectively. The amino acid residues concerning the coordination with Zn2+ in nuclease P1 are all conserved in nuclease Le1. Nuclease Le1 contains 8 half-cystine residues and 4 of them are located at the same places as those of nucleases P1 and S.
authors.affiliation: Department of Microbiology, College of Pharmacy, Nihon University, Funabashi, Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: F
authors.initials: F
authors.lastname: Kumagai
authors.forename: T
authors.initials: T
authors.lastname: Itagaki
authors.forename: N
authors.initials: N
authors.lastname: Inokuchi
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Iwama
authors.forename: K
authors.initials: K
authors.lastname: Ohgi
authors.forename: M
authors.initials: M
authors.lastname: Irie
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D008969:Molecular Sequence Data; D009708:Nucleotidases / Q000737:chemistry*; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012260:Ribonucleases; D009708:Nucleotidases; C040470:3'-nucleotidase