Direct coupling of microcolumn liquid chromatography with in-tube solid-phase microextraction for the analysis of antidepressant drugs.
The direct coupling of in-tube solid-phase microextraction (in-tube SPME) with microcolumn liquid chromatography (micro-LC) has been investigated for the analysis of antidepressants in human urine. The use of in-tube SPME has been clearly shown to be advantageous for the on-line coupling of the SPME method, as the sample pretreatment technique, with micro-LC as the separation technique. This is because much smaller amounts of the sample solutions, desorption solvents and the mobile phase are required compared with conventional SPME-LC systems. The parameters for preconcentration have been investigated for the extraction capillary with the newly developed 'wire-in-tube' configuration.
Saito
Y
Y
School of Materials Science, Toyohashi University of Technology, Japan.
Kawazoe
M
M
Hayashida
M
M
Jinno
K
K
D000928:Antidepressive Agents / Q000652:urine*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans
D000928:Antidepressive Agents