Homozygous deletion of the PTEN tumor-suppressor gene is not a feature in oral squamous cell carcinoma.
A recently identified putative tumor-suppressor gene, PTEN, at 10q23 has been described as mutated or homozygously deleted in many different human tumors. To determine the role of the homozygous deletion of this PTEN gene in oral squamous cell carcinoma (OSCC), we screened two cell lines derived from the latter tissue and 28 tumor samples from patients with OSCC, using a differential display polymerase chain reaction (PCR) system and, direct DNA sequencing methods. All of the nine exons of the PTEN could be successfully amplified using DNA from tumor tissues and the cell lines using this system. DNA sequencing confirmed the accuracy of the PCR procedures. However, none of the samples, either from the cancer tissues or from the cell lines, showed homozygous deletion of PTEN. These data suggest that homozygous deletion of the PTEN gene is unlikely to be a feature of OSCCs.
Chen
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Q
Faculty of Dentistry, University of Hong Kong, Hong Kong. qchen@khusua.hku.hk
Samaranayake
L P
LP
Zhou
H
H
Xiao
L
L
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D025521:Tumor Suppressor Proteins
D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human