PAPIN. A novel multiple PSD-95/Dlg-A/ZO-1 protein interacting with neural plakophilin-related armadillo repeat protein/delta-catenin and p0071.
A neural plakophilin-related armadillo repeat protein (NPRAP)/delta-catenin interacts with one of Alzheimer disease-related gene products, presenilin 1. We have previously reported the interaction of NPRAP/delta-catenin with synaptic scaffolding molecule, which is involved in the assembly of synaptic components. NPRAP/delta-catenin also interacts with E-cadherin and beta-catenin and is implicated in the organization of cell-cell junctions. p0071, a ubiquitous isoform of NPRAP/delta-catenin, is localized at desmosomes in HeLa and A431 cells and at adherens junctions in Madin-Darby bovine kidney cells. We have identified here a novel protein interacting with NPRAP/delta-catenin and p0071 and named this protein plakophilin-related armadillo repeat protein-interacting PSD-95/Dlg-A/ZO-1 (PDZ) protein (PAPIN). PAPIN has six PDZ domains and binds to NPRAP/delta-catenin and p0071 via the second PDZ domain. PAPIN and p0071 are ubiquitously expressed in various tissues and are localized at cell-cell junctions in normal rat kidney cells and bronchial epithelial cells. PAPIN may be a scaffolding protein connecting components of epithelial junctions with p0071.
Deguchi
M
M
Takai Biotimer Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, c/o JCR Pharmaceuticals Co. Ltd., Kobe 651-2241, Japan.
Iizuka
T
T
Hata
Y
Y
Nishimura
W
W
Hirao
K
K
Yao
I
I
Kawabe
H
H
Takai
Y
Y
D048868:Adaptor Proteins, Signal Transducing; D000544:Alzheimer Disease / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D051186:Armadillo Domain Proteins; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D051177:Catenins; D002417:Cattle; D015815:Cell Adhesion Molecules; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004285:Dogs; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins; D051191:Plakophilins; D053764:Presenilin-1; D051381:Rats; D016415:Sequence Alignment; D000095002:Delta Catenin
D048868:Adaptor Proteins, Signal Transducing; D051186:Armadillo Domain Proteins; D002352:Carrier Proteins; D051177:Catenins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C423943:PDZD2 protein, human; C414604:PDZD2 protein, rat; C105677:PKP4 protein, human; C508853:PSEN1 protein, human; D010750:Phosphoproteins; D051191:Plakophilins; D053764:Presenilin-1; D000095002:Delta Catenin