Isolation and localization of type IIb Na/Pi cotransporter in the developing rat lung.
Differential display analysis of rat lung at different developmental stages identified a fragment, HG80, which appeared on embryonic day 16.5 and thereafter. A full-length cDNA derived from a cDNA library of newborn rat lung probed with HG80 was the rat counterpart of sodium-dependent phosphate transporter type IIb and was designated rNaPi IIb. In situ hybridization showed that rNaPi IIb was expressed in type II alveolar cells, suggesting a role in the synthesis of surfactant in the alveoli. The time-dependent changes in localization of this gene in the developing lung and its possible use as a type II pneumocyte marker are discussed.
Hashimoto
M
M
First Department of Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Wang
D Y
DY
Kamo
T
T
Zhu
Y
Y
Tsujiuchi
T
T
Konishi
Y
Y
Tanaka
M
M
Sugimura
H
H
D000818:Animals; D000831:Animals, Newborn; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017422:Sequence Analysis, DNA; D050608:Sodium-Phosphate Cotransporter Proteins; D050610:Sodium-Phosphate Cotransporter Proteins, Type II; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb; D027981:Symporters
D002352:Carrier Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; C493559:Slc34a2 protein, rat; D050608:Sodium-Phosphate Cotransporter Proteins; D050610:Sodium-Phosphate Cotransporter Proteins, Type II; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb; D027981:Symporters; D012313:RNA