title: Resorbable calcium phosphate particles as a carrier material for bone marrow in an ovine segmental defect.
abstract: Resorbable calcium phosphate ceramics are only osteoconductive; therefore, their combination with osteogenic substances may lead to stimulation of bone healing. In the present study this combination, using autologous bone marrow, was investigated. In 31 sheep, a 3-cm tibial segmental defect was created and stabilized with an intramedullary nail. The animals were divided into four groups: empty defects (group 1, n = 7), and defects filled with 10-mL dense resorbable calcium phosphate particles (group 2, n = 8), with 10-mL particles soaked in bone marrow (group 3, n = 8), or with 10-mL autologous bone (group 4, n = 8). On evaluation after 12 weeks, significantly higher values were seen in group 3 than in group 2 for callus volume (p = .016), bone mineral density ratio (p = .03), bone mineral content ratio (p = .04), torsional strength (p = .005), and torsional stiffness (p = .01). For all end points, the outcome of group 3 was lower than that of group 4. In the histology, there was direct contact between newly formed bone and remnants of the particles. There were no signs of inflammatory reactions. Although a stimulatory effect of bone marrow was seen, the combination of resorbable calcium phosphate particles with bone marrow does not provide an alternative for autologous bone grafting.
authors.affiliation: Department of Traumatology, Academic Hospital Vrije Universiteit, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. tj.blokhuis@azvu.nl
authors.forename: T J
authors.initials: TJ
authors.lastname: Blokhuis
authors.forename: B W
authors.initials: BW
authors.lastname: Wippermann
authors.forename: F C
authors.initials: FC
authors.lastname: den Boer
authors.forename: A
authors.initials: A
authors.lastname: van Lingen
authors.forename: P
authors.initials: P
authors.lastname: Patka
authors.forename: F C
authors.initials: FC
authors.lastname: Bakker
authors.forename: H J
authors.initials: HJ
authors.lastname: Haarman
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D015519:Bone Density; D016026:Bone Marrow Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000293:injuries / Q000473:pathology / Q000601:surgery*; D002130:Calcium Phosphates; D002516:Ceramics; D005260:Female; D008422:Materials Testing; D010012:Osteogenesis; D012756:Sheep; D014182:Transplantation, Autologous
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous