Immunocytochemical strategies for electron microscopy: choice or compromise.
This review attempts to describe the major immunocytochemical strategies that are in current use for transmission electron microscopy. Emphasis is placed on suggesting the most appropriate application of each technique that is described. The review is not intended to identify the microscopist's 'cure all' technique for immunocytochemistry but rather to describe what techniques are available and what are their relative merits. Expensive equipment and technically complicated methods are not always necessary to collect robust data. I hope to present a review that will enable its readers to select the most profitable technique (or compromise) available, to reflect both their resources and their ability to address their biological questions with vigour.
Skepper
J N
JN
University of Cambridge, Multi-Imaging Centre, Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK. jns1000@hermes.cam.ac.uk
D000818:Animals; D007150:Immunohistochemistry / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D016610:Tissue Embedding / Q000379:methods; D016707:Tissue Fixation / Q000379:methods