title: Dual HDL/total cholesterol test: a single-tube, homogeneous assay for sequential measurement of HDL cholesterol and total cholesterol.
abstract: The quantitation of cholesterol in lipoprotein subfractions is valuable in estimating the risk for coronary artery disease, but requires multiple tests. We describe a relatively simple procedure, referred to as the dual HDL/total cholesterol (DHT) assay, which allows the sequential measurement of HDL cholesterol (HDL-C) and total cholesterol (total-C) in a single tube. HDL-C is first measured using a homogeneous assay that utilizes an anti-apolipoprotein B [apo(B)] antibody, which sterically blocks the enzymatic measurement of cholesterol on the non-HDL subfractions. Next, deoxycholate is added, which disrupts the antibody-apo(B) complex and allows the subsequent enzymatic measurement of the remaining cholesterol in the non-HDL subfractions. The DHT assay has an acceptable analytical performance and yields results similar to standard methods: for HDL-C, y(DHT) = 0.98x + 0.19, r=0.90; for total-C, y(DHT) = 1.11x - 0.09, r=0.99. In summary, the DHT assay is a homogeneous assay for both HDL-C and total-C, and provides a simple and cost-effective method for screening for hyperlipidaemia.
authors.affiliation: Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Sampson
authors.forename: G
authors.initials: G
authors.lastname: Csako
authors.forename: A T
authors.initials: AT
authors.lastname: Remaley
mesh_terms: D000906:Antibodies / Q000378:metabolism; D001055:Apolipoproteins B / Q000276:immunology; D001663:Bilirubin / Q000378:metabolism; D002624:Chemistry, Clinical / Q000379:methods*; D002784:Cholesterol / Q000032:analysis* / Q000097:blood*; D003840:Deoxycholic Acid / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000175:diagnosis; D008075:Lipoproteins, HDL / Q000032:analysis* / Q000097:blood*; D008403:Mass Screening / Q000191:economics / Q000379:methods; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000906:Antibodies; D001055:Apolipoproteins B; D006454:Hemoglobins; D008075:Lipoproteins, HDL; D003840:Deoxycholic Acid; D002784:Cholesterol; D001663:Bilirubin