A new affinity ligand for the isolation of a single 'feruloyl esterase' (FAE-III) from Aspergillus niger.
8-Aminooctyl 5'-S-coniferyl-5'-deoxy-thio-alpha-L-arabinofuranoside has been synthesised and shown to be a selective affinity ligand for the feruloyl esterase III of Aspergillus niger. The hydrolyses of methyl 5-O-coumaroyl, feruloyl, or sinapoyl alpha-L-arabinofuranosides by this enzyme proceed at comparable rates.
Zhao
Y
Y
Department of Chemistry, University of Illinois at Chicago, 60607-7061, USA.
Sinnott
M L
ML
D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development; D002265:Carboxylic Ester Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D007700:Kinetics; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D013379:Substrate Specificity; D013865:Thioglycosides / Q000138:chemical synthesis* / Q000378:metabolism
C414941:8-aminooctyl 5'-S-coniferyl-5'-deoxythioarabinofuranoside; D008024:Ligands; D013865:Thioglycosides; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase