title: Iron, ferritin, transferrin, and transferrin receptor in the adult rat retina.
abstract: OBJECTIVE
  The retina and other tissues need iron to survive. However, the normal iron metabolism in rodent retinas had not been characterized. This study was intended to investigate iron and iron homeostasis protein (ferritin, transferrin [Tf] and transferrin receptor [Tf-R]) distribution in 20- to 55-day-old rat retinas.
  
  
  METHODS
  Iron was revealed on retinal sections directly by proton-induced x-ray emission (PIXE) and indirectly by electron microscopy (EM). Ferritin, Tf, and Tf-R proteins were localized by immunohistochemistry. Transferrin expression was localized by in situ hybridization (ISH). Transferrin and ferritin proteins and mRNA were analyzed by Western blot analysis and reverse transcription-polymerase chain reaction (RT-PCR), respectively.
  
  
  RESULTS
  Iron is widely and unevenly distributed throughout the adult rat retina. The highest concentration was observed by PIXE in the choroid and the retinal pigmented epithelial cell (RPE) layer, and in inner segments of photoreceptors (IS). Outer segments of photoreceptors (OS) also contain iron. EM studies suggested the presence of iron inclusions inside the photoreceptor discs. Choroid, RPE, and IS showed a strong immunoreactivity for ferritin. Transferrin accumulated mainly in the IS and OS areas and in RPE cells but can also be detected slightly in retinal capillaries. Western blot analysis for Tf and ferritin confirmed their presence in the adult neural retina. By RT-PCR, H- and L-chains of ferritin and Tf mRNAs were expressed in neural retina, but the main sites of Tf synthesis observed by ISH were the RPE and choroid cell layers. Tf-R immunoreactivity was detected in the ganglion cell layer, inner nuclear layer, outer plexiform layer, IS, RPE, and choroid. These results were similar for all stages studied.
  
  
  CONCLUSIONS
  For the first time, the present study characterized both iron and iron homeostasis proteins in rodent retinas. In the outer retina, iron and ferritin shared the same distribution patterns. In contrast, Tf, mainly synthesized by RPE cells and detected in OS and IS areas, probably helps to transport iron to photoreceptors through their Tf-R. This is a likely pathway for filling iron needs in the outer retina.
authors.affiliation: Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg.
authors.forename: M G
authors.initials: MG
authors.lastname: Yefimova
authors.forename: J C
authors.initials: JC
authors.lastname: Jeanny
authors.forename: X
authors.initials: X
authors.lastname: Guillonneau
authors.forename: N
authors.initials: N
authors.lastname: Keller
authors.forename: J
authors.initials: J
authors.lastname: Nguyen-Legros
authors.forename: C
authors.initials: C
authors.lastname: Sergeant
authors.forename: F
authors.initials: F
authors.lastname: Guillou
authors.forename: Y
authors.initials: Y
authors.lastname: Courtois
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015342:DNA Probes / Q000737:chemistry; D004577:Electron Probe Microanalysis; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D017403:In Situ Hybridization; D007501:Iron / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D011863:Radioimmunoassay; D051381:Rats; D020318:Rats, Long-Evans; D011922:Rats, Mutant Strains; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000378:metabolism*; D012160:Retina / Q000378:metabolism* / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014168:Transferrin / Q000235:genetics / Q000378:metabolism*
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D014168:Transferrin; D005293:Ferritins; D007501:Iron