Autocrine growth effect of IL-8 and GROalpha on a human pancreatic cancer cell line, Capan-1.
A human pancreatic cancer cell line, Capan-1, secretes the chemokines interleukin-8 (IL-8) and growth-related oncogene alpha (GROalpha). Capan-1 cells also express the chemokine receptor 2 (CXCR2), which is a Gialpha-protein coupled receptor. Growth of Capan-1 cells was inhibited when anti-IL-8 or anti-GROalpha monoclonal antibody was added into the culture medium. Pertussis toxin, which blocks Gialpha also demonstrated a growth-inhibitory effect on Capan-1 cells. These results indicated that IL-8 and GROalpha act on Capan-1 cells as growth factors in an autocrine manner through CXCR2.
Takamori
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First Department of Surgery, Kumamoto University School of Medicine, Japan. takamori@mail.kuh.kamamoto-u.ac.jp
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D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000235:genetics / Q000502:physiology*; D006131:Growth Inhibitors / Q000235:genetics / Q000502:physiology*; D006133:Growth Substances / Q000235:genetics / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000235:genetics / Q000502:physiology*; D010190:Pancreatic Neoplasms; D037342:Pertussis Toxin; D023063:Receptors, Interleukin-8B / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000911:Antibodies, Monoclonal; C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006131:Growth Inhibitors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D023063:Receptors, Interleukin-8B; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin