title: Novel HLA-B*15 alleles identified in potential marrow donors.
abstract: Several methods for low-resolution class I typing of potential bone marrow donors are available. The National Marrow Donor Program (NMDP) has initiated pilot projects for large-scale DNA-based class I typing to initially characterize donors. Sequence-specific oligonucleotide probe hybridization and sequence-specific primer polymerase chain reaction (PCR) screening of 3,500 NMDP potential donors suggested the presence of variants of known HLA-B*15 variants in 3 donors. PCR products encompassing HLA-B locus exons 1 through 3 were prepared and subcloned. Sequencing revealed 3 alleles differing from known HLA-B*15 alleles by nucleotide substitutions resulting in predicted novel HLA-B antigens. The new alleles occur in distinct ethnic groups. These findings further illustrate the substantial genetic variation present at the HLA-B locus within human populations.
authors.affiliation: Department of Pathology, University of New Mexico, Albuquerque 87131-5051, USA. twilliams@salud.unm.edu
authors.forename: T M
authors.initials: TM
authors.lastname: Williams
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Bassinger
authors.forename: G D
authors.initials: GD
authors.lastname: Montoya
authors.forename: J
authors.initials: J
authors.lastname: Yee
authors.forename: B B
authors.initials: BB
authors.lastname: Griffith
authors.forename: J
authors.initials: J
authors.lastname: Kearns
authors.forename: M
authors.initials: M
authors.lastname: McKeen
authors.forename: S
authors.initials: S
authors.lastname: Birkos
authors.forename: I S
authors.initials: IS
authors.lastname: Gourley
authors.forename: M
authors.initials: M
authors.lastname: Kamoun
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D044383:Black People / Q000235:genetics; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005091:Exons / Q000276:immunology; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D059910:HLA-B15 Antigen; D006650:Histocompatibility Testing; D006801:Humans; D007198:Indians, North American / Q000235:genetics; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D014019:Tissue Donors; D014481:United States; D001741:Black or African American
chemical_list: D015235:HLA-B Antigens; D059910:HLA-B15 Antigen