1,1-Diphenyl-2-picrylhydrazyl radical-scavenging compounds from soybean miso and antiproliferative activity of isoflavones from soybean miso toward the cancer cell lines.
Guided by their DPPH radical-scavenging activity, nine compounds were isolated from soybean miso. Of these, 8-hydroxydaidzein, 8-hydroxygenistein and syringic acid had as high DPPH radical-scavenging activity as that of alpha-tocopherol. The antiproliferative activity of four of the isolated isoflavones toward three cancer cell lines was examined. 8-Hydroxygenistein showed the highest activity (IC50=5.2 microM) toward human promyelocytic leukemia cells (HL-60).
Hirota
A
A
Laboratory of Applied Microbiology, School of Food and Nutritional Sciences, University of Shizuoka, Japan. hirotaa@u-shizuoka-ken.ac.jp
Taki
S
S
Kawaii
S
S
Yano
M
M
Abe
N
N
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D015764:Bepridil / Q000031:analogs & derivatives*; D001713:Biphenyl Compounds; D002455:Cell Division / Q000187:drug effects*; D003373:Coumaric Acids / Q000378:metabolism / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005285:Fermentation; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D005609:Free Radicals; D019833:Genistein / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D010851:Picrates; D013025:Glycine max / Q000737:chemistry* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D005609:Free Radicals; D007529:Isoflavones; D010851:Picrates; D015764:Bepridil; C004931:1,1-diphenyl-2-picrylhydrazyl; D019833:Genistein