Micelle-mediated extraction for concentrating hydrophobic organic compounds.
An extraction method based on polymer-induced phase separation of aqueous micellar solutions of octyl-beta-D-thioglucoside (OTG) was assessed for concentrating hydrophobic analytes. Various hydrophobic compounds such as polycyclicaromatic hydrocarbons, alkylbenzenes, alkylphenols, chlorobenzenes, chlorophenols, phthalic esters, pesticides, and steroid hormones could be efficiently concentrated into a small volume of surfactant-rich phase, while hydrophilic matrix components remained in the bulk aqueous phase. The surfactant-rich phase containing concentrated OTG could be directly introduced into the hygro-organic mobile phase of high-performance liquid chromatography with ultra-violet photometric detection. The application of this method greatly enhanced the signal intensity in the chromatogram while reducing the interference of matrix components.
Saitoh
T
T
Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Japan. saitoh@numse.nagoya-u.ac.jp
Matsudo
T
T
Matsubara
C
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008823:Micelles; D009930:Organic Chemicals / Q000737:chemistry* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013863:Thioglucosides / Q000737:chemistry; D014867:Water
D008823:Micelles; D009930:Organic Chemicals; D013863:Thioglucosides; D014867:Water; C042989:n-octyl-beta-D-thioglucopyranoside