title: Assisted folding of D-glyceraldehyde-3-phosphate dehydrogenase by trigger factor.
abstract: The Escherichia coli trigger factor is a peptidyl-prolyl cis-trans isomerase that catalyzes proline-limited protein folding extremely well. Here, refolding of D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the presence of trigger factor was investigated. The regain of activity of GAPDH was markedly increased by trigger factor after either long- or short-term denaturation, and detectable aggregation of GAPDH intermediates was prevented. In both cases, time courses of refolding of GAPDH were decelerated by trigger factor. The reactivation yield of GAPDH showed a slow down-turn when molar ratios of trigger factor to GAPDH were above 5, due to tight binding between trigger factor and GAPDH intermediates. Such inactive bound GAPDH could be partially rescued from trigger factor by addition of reduced alphaLA as competitor, by further diluting the refolding mixture, or by disrupting hydrophobic interactions in the complexes. A model for trigger factor assisted refolding of GAPDH is proposed. We also suggest that assisted refolding of GAPDH is due mainly to the chaperone function of trigger factor.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, China.
authors.forename: G C
authors.initials: GC
authors.lastname: Huang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Li
authors.forename: J M
authors.initials: JM
authors.lastname: Zhou
authors.forename: G
authors.initials: G
authors.lastname: Fischer
mesh_terms: D004789:Enzyme Activation; D029968:Escherichia coli Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry* / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding
chemical_list: D029968:Escherichia coli Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C403324:trigger factor, E coli; D019696:Peptidylprolyl Isomerase