Alteration of the self-incompatibility phenotype in Brassica by transformation of the antisense SLG gene.
Self-incompatible (SI) Brassica rapa (syn. B. campestris) was transformed with an antisense SLG gene by using SLG8 cDNA isolated from the B. campestris S8 homozygote. Two transformed lines were obtained and analyzed. Northern blot and Western blot analyses revealed that endogenous SLG and SRK were greatly reduced of the transcriptional and translational levels in the transformant. Pollination experiments confirmed that their SI phenotype had broken down. In addition, the progeny with the antisense SLG gene, resulting from self- or cross-pollination of the transgenic plant, also showed the self-compatible phenotype. The breakdown of SI in the tranformants was due to the change in property of the stigma and not of the pollen. These results provide strong evidence that SLG and/or SRK is implicated in the pollen-stigma recognition of SI and that they act only as stigmatic factors.
Shiba
H
H
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
Kimura
N
N
Takayama
S
S
Hinata
K
K
Suzuki
A
A
Isogai
A
A
D001937:Brassica / Q000033:anatomy & histology / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D016373:DNA, Antisense / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics*; D015151:Immunoblotting; D007178:Inbreeding; D010641:Phenotype; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000502:physiology*; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics*; D018749:RNA, Plant / Q000032:analysis; D014170:Transformation, Genetic; D019076:Transgenes
D016373:DNA, Antisense; D006023:Glycoproteins; D010940:Plant Proteins; D018749:RNA, Plant; C064156:self-incompatibility locus specific glycoprotein, Brassica; D011494:Protein Kinases; C079832:S-receptor kinase