Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography.
A DEAE membrane radial column (4x5 cm) was used to purify human prothrombin from Nitschmann fraction III and performances of the column were investigated. Sample flow-rate of 20-30 ml/min (i.e., 24-36 bed volumes/h) and elution velocity up to 40 ml/min (i.e., 48 bed volumes/h) were obtained without compromising the separation efficiency. Its breakthrough capacity was one-third to half that of the commercial DEAE Sepharose Fast Flow media. It was concluded that the novel column is an attractive alternate to traditional axial column.
Sun
T
T
Institute of Transfusion Medicine, Beijing, China.
Chen
G
G
Liu
Y
Y
Bu
F
F
Wen
M
M
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008567:Membranes, Artificial; D011516:Prothrombin / Q000302:isolation & purification*
D008567:Membranes, Artificial; D011516:Prothrombin