Food restriction prevents advanced glycation end product accumulation and retards kidney aging in lean rats.
ABSTRACT.: Tissue content of advanced glycation end products (AGE) increases with age and contributes to the changes in structure and function of the renal and cardiovascular systems. The effect of chronic food restriction on this AGE accumulation was investigated in lean WAG/Rij rats. A 30% food restriction performed from 10 to 30 mo in female rats reduced their mean body weight from 240 +/- 7 to 160 +/- 12 g, but did not modify their survival. AGE collagen content increased from 14.3 +/- 5.5 to 104.7 +/- 13.0 arbitrary units per microgram (AU/microg) of hydroxyproline (OHPro) in kidney between 10 and 30 mo, and from 9.7 +/- 1.2 to 310.6 +/- 34.6 AU/microg OHPro in the abdominal aorta. Food restriction reduced AGE accumulation to 21.4 +/- 3.3 and 74.6 +/- 16.5 AU/microg OHPro in kidney and aorta of 30-mo-old animals. Similar results were found for collagen prepared from isolated glomeruli (7.8 +/- 1.2, 81.2 +/- 16.1, and 10.3 +/- 4.3 AU/microg OHPro in 10-mo, 30-mo, and restricted 30-mo-old rats). Reduction of intrarenal and arterial AGE accumulation by food restriction was confirmed by immunostaining in optical microscopy. Age-related changes in arterial and kidney structures as polyuria and proteinuria were mainly prevented by food restriction. These data indicate that chronic food restriction reduces the accumulation of AGE and preserves the structure and function of the renal and cardiovascular systems in learn rats, although it did not affect survival of the animals between 10 and 30 mo.
Teillet
Laurent
L
Service de Biologie Cellulaire, Commissariat à l'Energie Atomique/Saclay, Gif-sur-Yvette, France.|AP-HP, Hôpital Sainte-Périne, Paris, France.
Verbeke
Philippe
P
Université Paris VII, Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Paris, France.
Gouraud
Sabine
S
Service de Biologie Cellulaire, Commissariat à l'Energie Atomique/Saclay, Gif-sur-Yvette, France.
Bakala
Hilaire
H
Université Paris VII, Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Paris, France.
Borot-Laloi
Caroline
C
Université Paris VII, Laboratoire de Biologie et Biochimie Cellulaire du Vieillissement, Paris, France.
Heudes
Didier
D
Institut National de la Santé et de la Recherche Médicale U430, Hôpital Broussais, Paris, France.
Bruneval
Patrick
P
Institut National de la Santé et de la Recherche Médicale U430, Hôpital Broussais, Paris, France.
Corman
Bruno
B
Service de Biologie Cellulaire, Commissariat à l'Energie Atomique/Saclay, Gif-sur-Yvette, France.
D000818:Animals; D001823:Body Composition; D001835:Body Weight; D004797:Enzyme-Linked Immunosorbent Assay; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007158:Immunologic Techniques; D007668:Kidney / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D016019:Survival Analysis
D017127:Glycation End Products, Advanced