Anti-HBV effect of targeted antisense RNA against HBV C gene.
OBJECTIVE
To investigate the anti-HBV effect of targeted antisense RNA to hepatic cells.


METHODS
pREP4-aC which would transcript antisense RNA against HBV C gene in eukaryotic cells were delivered into 2.2.15 cells by glactosylated poly-L-lisine (Gal-PLL), and the positive cells were selected. HBsAg, HBeAg and HBV DNA produced by 2. 2.15 cells were detected with ELISA or Southern blot during the experiment, and the cytotoxicity of targeted antisense on 2.2.15 cells was observed.


RESULTS
The inhibition effect on HBsAg, HBeAg and HBV DNA occurred at the 24th hour after delivery and reached the highest level at the 6th day, and kept at lest two months. No cytotoxicity on 2.2.15 cells was observed.


CONCLUSIONS
The targeted antisense against HBV C gene by delivery of Gal-PLL could effectively inhibit the antigen expression of HBV and DNA replication.
Zhang
J
J
Department of Infectious Disease, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan Province 646000, China.
Chen
F
F
Zhong
S
S
Tang
K
K
Shi
X
X
Wang
M
M
Peng
J
J
D000998:Antiviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D016372:RNA, Antisense / Q000494:pharmacology*
D000998:Antiviral Agents; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D016372:RNA, Antisense