Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection.
In this study, antiviral effect of black seed oil (BSO) from Nigella sativa was investigated using murine cytomegalovirus (MCMV) as a model. The viral load and innate immunity mediated by NK cells and M&phi; during early stage of the infection were analyzed. Intraperitoneal (i.p.) administration of BSO to BALB/c mice, a susceptible strain of MCMV infection, strikingly inhibited the virus titers in spleen and liver on day 3 of infection with 1x10(5) PFU MCMV. This effect coincided with an increase in serum level of IFN-gamma. Although BSO treatment decreased both number and cytolytic function of NK cells on day 3 of infection, it increased numbers of M&phi; and CD4(+) T cells. On day 10 of infection, the virus titer was undetectable in spleen and liver of BSO-treated mice, while it was detectable in control mice. Although spleen of both control and BSO-treated mice showed similar CTL activities on day 10 after infection, serum level of IFN-gamma in BSO-treated mice was higher. Furthermore, BSO treatment upregulated suppressor function of M&phi; in spleen. These results show that BSO exhibited a striking antiviral effect against MCMV infection which may be mediated by increasing of M&phi; number and function, and IFN-gamma production.
Salem
M L
ML
Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. mohamed_84@hotmail.com
Hossain
M S
MS
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000097:blood / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008099:Liver / Q000821:virology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010938:Plant Oils / Q000494:pharmacology*; D010946:Plants, Medicinal; D013154:Spleen / Q000276:immunology / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology; D019562:Viral Load
D000998:Antiviral Agents; D010938:Plant Oils; D007371:Interferon-gamma