Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex.
The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H(t(His)):gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD(t) were determined. As antigens, purified soluble recombinant forms of the gH(t(His)):gL complex produced by insect cells and of gD(t) produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH(t(His)): gL-specific IgG. Using peripheral blood (PB) T cells, gH(t(His)):gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH(t(His)):gL contained high levels of interferon-gamma and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.
Westra
Douwe F
DF
Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Verjans
Georges M G M
GMGM
Rotterdam Eye Hospital2 and Institute of Virology3, Erasmus University Rotterdam, Rotterdam, The Netherlands.
Osterhaus
Albert D M E
ADME
Rotterdam Eye Hospital2 and Institute of Virology3, Erasmus University Rotterdam, Rotterdam, The Netherlands.
van Kooij
Adriaan
A
Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Welling
Gjalt W
GW
Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Scheffer
Albert Jan
AJ
Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
The
T Hauw
TH
Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Welling-Wester
Sytske
S
Department of Medical Microbiology1 and Department of Clinical Immunology4, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D015847:Interleukin-4; D007371:Interferon-gamma