title: Differential distribution and defence involvement of antimicrobial peptides in mussel.
abstract: In previous papers, we characterised 3 types of 4-kDa, cysteine-rich, cationic antimicrobial peptides: MGDs (for Mytilus galloprovincialis defensins), mytilins and myticins, which are abundant in the mussel hemocytes. In the present work, we revealed a differential distribution of the cells expressing the different genes. In addition, using confocal and electron microscopy, we confirmed that defensins and mytilins were partially located in different sub-types of circulating hemocytes although the peptides can be located in the same cell, and even in the same granule. We also demonstrated that mytilins exert their microbicidal effect within the cells through the process of phagosome-mytilin granule fusion leading to the co-location of ingested bacteria and mytilins.
authors.affiliation: Laboratoire d'Endocrinologie des Annélides, Groupe de Neuroimmunité des Hirudinées, UPRES A 8017 CNRS, Université des Sciences et Technologies de Lille, France.
authors.forename: G
authors.initials: G
authors.lastname: Mitta
authors.forename: F
authors.initials: F
authors.lastname: Vandenbulcke
authors.forename: T
authors.initials: T
authors.lastname: Noël
authors.forename: B
authors.initials: B
authors.lastname: Romestand
authors.forename: J C
authors.initials: JC
authors.lastname: Beauvillain
authors.forename: M
authors.initials: M
authors.lastname: Salzet
authors.forename: P
authors.initials: P
authors.lastname: Roch
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides; D049872:Bivalvia / Q000276:immunology*; D001798:Blood Proteins / Q000032:analysis / Q000235:genetics*; D016384:Consensus Sequence; D023082:Defensins; D015870:Gene Expression / Q000276:immunology; D006434:Hemocytes / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000276:immunology; D011506:Proteins / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014733:Vibrio / Q000276:immunology; D014735:Vibrio Infections / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D023082:Defensins; D011506:Proteins; D012333:RNA, Messenger; C400201:myticin; C408470:mytilin