Establishment and usefulness of an anti-human CD57 IgG1 monoclonal antibody.
In the present study we established a new monoclonal antibody, JNK-1, which recognizes all cells recognized by CD57/HNK-1 mAb. JNK-1 and CD57 mAbs inhibited the binding of each other, suggesting that the molecules they recognize are either identical or sufficiently close to cause steric hindrance in the binding assay. JNK-1 mAb detected the 110-kDa protein, which is identical to the protein recognized by CD57/HNK-1 mAb in Western immunoblot analysis combined with immunoprecipitation. Therefore, JNK-1 mAb appears to recognize homogeneous molecules identified by the currently available CD57 mAb. Notably, JNK-1 mAb is composed of mouse IgG1 heavy chains, and thus can be used easily in immunoprecipitation, which cannot easily be performed with the available CD57 mAb because it is an IgM isotype. Thus, JNK-1, which is an IgG isotype, may present a useful tool to elucidate the CD57 protein.
Tajima
J
J
Pharmacology Division, National Cancer Center Research Institute, 5-1-1, Tsukiji, Chuo-ku, 104-0045, Tokyo, Japan.
Heike
Y
Y
Kato
K
K
Ikarashi
Y
Y
Asada-Mikami
R
R
Yoshida
M
M
Kasahara
T
T
Wakasugi
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000145:classification / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D018959:CD57 Antigens / Q000276:immunology*; D003080:Cold Temperature; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000145:classification / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D019002:CD56 Antigen; D018959:CD57 Antigens; D007074:Immunoglobulin G