title: Induction of tryptophan 2,3-dioxygenase in the mouse endometrium during implantation.
abstract: Indoleamine 2,3-dioxygenase (IDO) is expressed in trophoblasts and defends the conceptus against rejection by reducing the tryptophan level and suppressing the T cell activity. We isolated a cDNA for tryptophan 2,3-dioxygenase (TDO), another key catabolizing enzyme of tryptophan, from a mouse uterus cDNA library enriched with pregnancy-induced genes. Northern blot and in situ hybridization analyses demonstrated that the TDO mRNA was induced in the decidualized stromal cells around the implanted embryo at the time of implantation. The expression was then upregulated and primarily localized at the mesometrial decidua. TDO mRNA was induced by deciduoma formation as well as embryo transfer but not by ovarian steroid hormones. These findings demonstrated that TDO is induced in the endometrial stromal cells concomitant with decidualization and suggested its involvement in the implantation process by regulating the tryptophan level at the implantation site.
authors.affiliation: Department of Gynecology and Obstetrics, Department of Clinical Molecular Biology, Graduate School of Medicine, Kyoto University, Shogoin Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tatsumi
authors.forename: T
authors.initials: T
authors.lastname: Higuchi
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: H
authors.initials: H
authors.lastname: Egawa
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Fujii
authors.forename: J
authors.initials: J
authors.lastname: Fujita
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000378:metabolism; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000378:metabolism; D004717:Endometrium / Q000201:enzymology*; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015723:Gene Library; D017403:In Situ Hybridization; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D008297:Male; D051379:Mice; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014184:Transplantation, Homologous; D014364:Tryptophan / Q000378:metabolism; D014366:Tryptophan Oxygenase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D014364:Tryptophan; D014366:Tryptophan Oxygenase