title: Controllable stability of DNA-containing polyelectrolyte complexes in water-salt solutions.
abstract: Destruction of polyelectrolyte complexes (PECs) formed by DNA and synthetic polyamines of different structures was carried out by addition of low molecular weight electrolyte to PEC solution at different pHs. The dissociation was studied by the fluorescence quenching technique using the ability of cationic dye ethidium bromide to intercalate into free sites of DNA double helix followed by ignition of ethidium fluorescence. Structure of amine groups of the polycation was shown to be a decisive factor of PEC stability. PECs formed by polycations with quaternary amine groups, i.e., poly(N-alkyl-4-vinylpyridinium) bromides, poly(N, N-dimethyldiallylammonium) chloride, and ionene bromide, were pH independent and the least tolerant to destruction by the added salt. Primary amine groups of basic polypeptides poly-L-lysine hydrobromide and poly-L-arginine hydrochloride as well as synthetic polycation poly(vinyl-2-aminoethyl ether) provided the best stability of PECs in water-salt solutions under wide pH range. Moderate and pH-dependent stability was revealed for PECs included poly(N,N-dimethylaminoethylmethacrylate) with tertiary amine groups in the chain or branched poly(ethylenimine) with primary, secondary, and tertiary amine groups in the molecule. The data obtained appear to be the basis for design of DNA-containing PECs with given and controllable stability. The design may be accomplished not only by proper choice of polyamine of one or another type, but by using of tailor-made polycations with given composition of amine groups of different structure in the chain as well. Thus, quaternization of a part of tertiary amine groups of poly(N, N-dimethylaminoethylmethacrylate) resulted in expected decrease of stability of DNA-containing PECs in water-salt solutions. The destruction of PEC formed by random copolymer of 4-vinylpyridine and N-ethyl-4-vinylpyridinium bromide was pH sensitive and could be performed under pH and ionic strength closed to the physiological conditions. This result appears to be particularly promising for addressing DNA packed in PEC species to the target cell.
authors.affiliation: Chemistry Department, Moscow State University, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Izumrudov
authors.forename: M V
authors.initials: MV
authors.lastname: Zhiryakova
authors.forename: S E
authors.initials: SE
authors.lastname: Kudaibergenov
mesh_terms: D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004337:Drug Carriers; D004573:Electrolytes / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism*; D011199:Potentiometry; D012965:Sodium Chloride / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014867:Water / Q000494:pharmacology
chemical_list: D004337:Drug Carriers; D004573:Electrolytes; D011073:Polyamines; D014867:Water; D012965:Sodium Chloride; D004247:DNA