[Production and partial purification of the main hemolysin (pneumolysin) of Streptococcus pneumoniae].
The capacity of 36 Cuban strains of Streptococcus pneumoniae to produce pneumolysin was studied, and 94.4% of them were determined to be producers of that enzyme. One of the best producers was cultured at a great scale and the pneumolysin found in the supernatan was partially purified through an ion-exchange chromatography in mono-Q column. This method made it possible to recover the enzyme whose purity level increased by 4.39 with 100% output.
Toraño Peraza
G T
GT
era@ipk.sld.cu
Vilaseca Méndez
J C
JC
Tamargo Martínez
I
I
Pérez Monrrás
M
M
D001426:Bacterial Proteins; D003603:Cytotoxins / Q000096:biosynthesis / Q000302:isolation & purification*; D006801:Humans; D013296:Streptococcus pneumoniae / Q000378:metabolism*; D013301:Streptolysins / Q000096:biosynthesis / Q000302:isolation & purification*
D001426:Bacterial Proteins; D003603:Cytotoxins; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae