title: High insulin and low IGF-I plasma levels following pancreas transplantation in rats. Implications for bone and mineral metabolism.
abstract: Primary disturbances in mineral metabolism and deficiencies in insulin and insulin-like growth factor-I (IGF-I) have been implicated in the pathogenesis of diabetic osteopenia. This prompted us to investigate whether normal bone minerals and bone morphology are preserved after pancreas transplantation. To this end, 8 inbred rats (transplants) were compared with 9 sham-operated rats (controls) 20 months after orthotopic pancreas transplantation. While basal levels of insulin remained unaffected by transplantation, an oral glucose load elicited hyperinsulinemia (integrated incremental response: mean +/- SEM, 62+/-8 nmol l(-1) 60 min in transplants vs. 32+/-6 nmol l(-1) 60 min in controls; p<0.01) in the presence of normal glucose levels. Fecal and urinary excretion and fractional intestinal absorption of calcium, magnesium and phosphorus, net calcium absorption and the respective serum mineral levels were unchanged after transplantation, as were those of the calciotropic hormones. Serum osteocalcin and bone alkaline phosphatase remained unaffected, and urinary excretion of pyridinium and deoxypyridinium were unchanged. Fasting plasma IGF-I concentration was significantly decreased in transplants (930+/-42 ng ml(-1)) vs. control rats (1074+/-49 ng ml(-1); p < 0.05). Despite similar physical and chemical properties of bone in both groups, histomorphometry revealed slight osteopenia in transplant rats, as reflected by a 38% reduction in the cancellous bone area of the proximal tibial metaphysis. Plasma IGF-I levels were significantly correlated with bone mineral apposition rate (r=0.70, p<0.02), osteoblast perimeter (r=0.60, p<0.05) and osteoid perimeter (r=0.60, p<0.05). In conclusion, pancreas transplantation preserves physical and chemical properties of bone, but bone metabolism is not completely normal after transplantation, as evidenced by decreased cancellous bone. This might have resulted from the insulin resistance associated with the lowering of the plasma IGF-I level, which was correlated with the mineral apposition rate.
authors.affiliation: Department of Surgery, Friedrich Alexander University, Erlangen, Germany. hermann.kissler@stud.uni-erlangen.de
authors.forename: H J
authors.initials: HJ
authors.lastname: Kissler
authors.forename: G
authors.initials: G
authors.lastname: Hofmann
authors.forename: H
authors.initials: H
authors.lastname: Gepp
authors.forename: R G
authors.initials: RG
authors.lastname: Erben
authors.forename: P O
authors.initials: PO
authors.lastname: Schwille
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000652:urine; D005243:Feces; D005947:Glucose / Q000008:administration & dosage; D006946:Hyperinsulinism / Q000378:metabolism; D007328:Insulin / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D007408:Intestinal Absorption; D007515:Islets of Langerhans / Q000502:physiology; D008274:Magnesium / Q000378:metabolism / Q000652:urine; D008297:Male; D008903:Minerals / Q000378:metabolism*; D016035:Pancreas Transplantation; D010758:Phosphorus / Q000378:metabolism / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D008903:Minerals; D010758:Phosphorus; D007334:Insulin-Like Growth Factor I; D008274:Magnesium; D005947:Glucose; D002118:Calcium