Epitope mapping on N-terminal region of taenia solium paramyosin.
Epitope mapping of the amino-terminal 20aa sequence from Taenia solium paramyosin (TPmy), an immunodominant protein involved in the complex host-parasite relationship in human and porcine cysticercosis is reported. A 12-mer random peptide phage display library was screened with antibodies raised against a synthetic peptide corresponding to the amino-terminal 20aa sequence of TPmy, its highly immunodominant region. In total, 57 clones isolated in two panning conditions were analyzed, of which a single group of 14 sequences found in 25 clones shared a consensus motif showing structural similarity with the antigen Arg10-Thr16 region.
Gazarian
K G
KG
Department of Biotechnology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Aportado Postal 70228, Ciudad Universitaria 04510 D.F., Mexico. karlen@servidor.unam.mx
Gazarian
T G
TG
Solís
C F
CF
Hernández
R
R
Shoemaker
C B
CB
Laclette
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D018604:Epitope Mapping / Q000379:methods; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D011817:Rabbits; D013621:Taenia / Q000276:immunology*; D014335:Tropomyosin / Q000276:immunology*
D018985:Epitopes, B-Lymphocyte; D016056:Immunodominant Epitopes; D010455:Peptides; D014335:Tropomyosin