title: Isolation and characterisation of a chick cDNA encoding the RNA polymerase common subunit RPB6.
abstract: The RPB6 cDNA of chicken, encoding one of the small subunits common to all three nuclear DNA-dependent RNA polymerases, has been isolated from an expression cDNA library by screening with a differential display derived probe, representing a gene shown to be highly up-regulated in early heart development. The nucleotide sequence of the cDNA isolated predicts a protein sequence of 127 amino acids. This sequence shares 124 amino acids (98% homology) with the human RNA polymerase II subunit 14.4 kDa (RPB6) and hamster hRPB6 and 123 amino acids (97% homology) with Rattus norvegicus RNA polymerase II subunit RPB6. Other conserved motifs in this protein and potential functions of RPB6 are discussed.
authors.affiliation: School of Biomolecular and Biomedical Science, Griffith University, Nathan, Queensland, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Kaarbo
authors.forename: D I
authors.initials: DI
authors.lastname: Crane
authors.forename: W G
authors.initials: WG
authors.lastname: Murrell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D017124:Conserved Sequence; D006224:Cricetinae; D018076:DNA, Complementary; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; C120633:Polr2f protein, rat; D012321:DNA-Directed RNA Polymerases