title: Increased levels of apoptosis in the prefusion neural folds underlie the craniofacial disorder, Treacher Collins syndrome.
abstract: Treacher Collins syndrome (TCS) is an autosomal dominant disorder of human craniofacial development that results from loss-of-function mutations in the gene TCOF1. Although this gene has been demonstrated to encode the nucleolar phosphoprotein treacle, the developmental mechanism underlying TCS remains elusive, particularly as expression studies have shown that the murine orthologue, Tcof1, is widely expressed. To investigate the molecular pathogenesis of TCS, we replaced exon 1 of Tcof1 with a neomycin-resistance cassette via homologous recombination in embryonic stem cells. Tcof1 heterozygous mice die perinatally as a result of severe craniofacial anomalies that include agenesis of the nasal passages, abnormal development of the maxilla, exencephaly and anophthalmia. These defects arise due to a massive increase in the levels of apoptosis in the prefusion neural folds, which are the site of the highest levels of Tcof1 expression. Our results demonstrate that TCS arises from haploinsufficiency of a protein that plays a crucial role in craniofacial development and indicate that correct dosage of treacle is essential for survival of cephalic neural crest cells.
authors.affiliation: School of Biological Sciences and Department of Dental Medicine and Surgery, University of Manchester, UK.
authors.forename: J
authors.initials: J
authors.lastname: Dixon
authors.forename: C
authors.initials: C
authors.lastname: Brakebusch
authors.forename: R
authors.initials: R
authors.lastname: Fässler
authors.forename: M J
authors.initials: MJ
authors.lastname: Dixon
mesh_terms: D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000196:embryology / Q000473:pathology* / Q000648:ultrastructure; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D005145:Face / Q000196:embryology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D047908:Intracellular Signaling Peptides and Proteins; D008342:Mandibulofacial Dysostosis / Q000196:embryology / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008855:Microscopy, Electron, Scanning; D008957:Models, Genetic; D016296:Mutagenesis; D009154:Mutation; D009432:Neural Crest / Q000196:embryology / Q000473:pathology*; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C105442:TCOF1 protein, human; C491352:Tcof1 protein, mouse