title: The chemical synthesis of oligoribonucleotides with selectively placed 2'-O-phosphates.
abstract: A phosphoramidite, solid support method for the chemical synthesis of oligoribonucleotides containing 2'-O-phosphate at a selected position is presented. Synthesis of these oligoribonucleotides is based on uridine- and adenosine-(2'-O-phosphate)-3'-phosphoramidites, and a new condition for removal of 2'-O-phosphate protecting groups, which does not cleave internucleotide bonds. The structure of oligoribonucleotides with 2'-O-phosphate has been proven by enzymatic digestions and dephosphorylation by yeast 2'-phosphotransferase.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Kierzek
authors.forename: M A
authors.initials: MA
authors.lastname: Steiger
authors.forename: S L
authors.initials: SL
authors.lastname: Spinelli
authors.forename: D H
authors.initials: DH
authors.lastname: Turner
authors.forename: E M
authors.initials: EM
authors.lastname: Phizicky
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D009843:Oligoribonucleotides; D010770:Phosphotransferases; D000241:Adenosine; D014529:Uridine