Enzymatic degradation of cell wall polysaccharides from mango (Mangifera indica L.) puree.
Ripe mango puree (Smith cultivar) was treated with fungal polysaccharidases containing pectinolytic, hemicellulolytic, and cellulolytic activities for 2 h at 50 degrees C. A loss of 30% of the cell wall material (CWM) was measured. CWM polysaccharides were hydrolyzed to varying degrees: 88, 65, and 65% of, respectively, galacturonic acid-, arabinose-, and rhamnose-containing polymers were hydrolyzed, whereas 50% of cellulose was degraded. After 30 min of treatment, the ethanol precipitation test on the serum was negative, indicating that pectic substances were rapidly hydrolyzed. Oligogalacturonic acids (degree of polymerization, 1-12) were observed in the serum. A viscosity drop of 90% was measured after 2 h, confirming the dominant role of pectic substances in puree viscosity.
Ollé
D
D
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Départment FLHOR, Montpellier, F-34032, France. olle@cirad.fr
Baron
A
A
Lozano
Y F
YF
Brillouet
J M
JM
D002473:Cell Wall / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*; D014783:Viscosity
D011134:Polysaccharides; D006026:Glycoside Hydrolases