title: Chemokines MCP-1 and RANTES in isolated rat pancreatic acinar cells treated with CCK and ethanol in vitro.
abstract: The role of cholecystokinin (CCK) and ethanol on the expression and secretion of monocyte chemoattractant protein-1 (MCP-1) and regulated on activation, normal T-cell expressed and secreted (RANTES) chemokines from isolated rat pancreatic acinar cells was investigated. CCK at concentrations of 1 nM and 100 nM and ethanol at concentrations of 75, 200, 400, and 600 mM were used to stimulate isolated acini. The levels of MCP-1 and RANTES in the incubation medium were determined by enzyme-linked immunoabsorbent assay (ELISA). In the control groups, MCP-1 and RANTES were secreted into the incubation medium, and both increased with time. MCP-1 increased from baseline 17.6 pg/ml to 74.1 pg/ml, whereas RANTES increased from 255.5 to 318.3 pg/ml at 390 min. CCK at 100 nM caused a sustained increase in MCP-1 levels to 89.6 pg/ml at 390 min in the incubation medium, whereas the levels of RANTES gradually decreased after 180 min and reached its lowest level at 390 min. Ethanol at a concentration of 600 mM increased the levels of RANTES in the incubation medium, but inhibited the levels of MCP-1 at all concentrations (75, 200, 400, and 600 mM). In summary, rat pancreatic acinar cells secrete MCP-1 and RANTES, and the stimulation of these chemokines by CCK and ethanol suggests that they may be involved in the pathogenesis of acute pancreatitis.
authors.affiliation: Molecular Medicine Research Groups, Plymouth Postgraduate Medical School, University of Plymouth, England. byang@plymouth.ac.uk
authors.forename: B M
authors.initials: BM
authors.lastname: Yang
authors.forename: A G
authors.initials: AG
authors.lastname: Demaine
authors.forename: A
authors.initials: A
authors.lastname: Kingsnorth
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D018932:Chemokine CCL2 / Q000032:analysis / Q000378:metabolism*; D018946:Chemokine CCL5 / Q000032:analysis / Q000378:metabolism*; D002766:Cholecystokinin / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012844:Sincalide / Q000494:pharmacology
chemical_list: D018932:Chemokine CCL2; D018946:Chemokine CCL5; D000431:Ethanol; D002766:Cholecystokinin; D012844:Sincalide