title: Sequence and transfection of BoLA-DRB3 cDNAs.
abstract: Bovine MHC (BoLA-) DRB3 alleles encoded by the DH8A, DH22A and DH24A class II haplotypes were cloned from cDNA and characterized by sequence analysis. Comparison with other full-length DRB3 sequences suggested that DRB3 alleles may have evolved through multiple lineages. All three BoLA-DRB3 alleles were shown to express on the surface of transfected cells, and the transfectants were used to define or confirm the class II specificity of a panel of monoclonal antibodies.
authors.affiliation: Division of Molecular Biology, Roslin Institute, Midlothian, UK.
authors.forename: G C
authors.initials: GC
authors.lastname: Russell
authors.forename: D C
authors.initials: DC
authors.lastname: Fraser
authors.forename: S
authors.initials: S
authors.lastname: Craigmile
authors.forename: R A
authors.initials: RA
authors.lastname: Oliver
authors.forename: B M
authors.initials: BM
authors.lastname: Dutia
authors.forename: E J
authors.initials: EJ
authors.lastname: Glass
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle / Q000235:genetics* / Q000276:immunology*; D018076:DNA, Complementary / Q000235:genetics*; D019143:Evolution, Molecular; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics*; D059826:HLA-DRB3 Chains; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D008969:Molecular Sequence Data; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D006684:HLA-DR Antigens; D059826:HLA-DRB3 Chains; D000949:Histocompatibility Antigens Class II