The expression and structure of TGF-beta2 transcripts in rat muscles.
The transforming growth factor-beta2 (TGF-beta2) transcripts expressed in various tissues of rat were characterised by RT-PCR and the nucleotide sequence of the cDNAs determined. A transcript with an 84-nucleotide insert in the latency-associated peptide region, the long form, was found. The long form of TGF-beta2 was detected in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine. The 3' untranslated region of TGF-beta2 contained several putative AU-rich elements and multiple polyadenylation sites, indicating post-transcriptional regulation of TGF-beta2 synthesis. The levels of TGF-beta2 transcripts were estimated using semi-quantitative RT-PCR. They were down-regulated during muscle development and up-regulated after denervation. The long form constituted approximately 6% of the total TGF-beta2 messages in skeletal muscle.
Koishi
K
K
Department of Anatomy and Structural Biology, School of Medical Sciences, University of Otago, P.O. Box 913, Dunedin, New Zealand. kyoko.koishi@stonebow.otago.ac.nz
Dalzell
K G
KG
McLennan
I S
IS
D020413:3' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D017398:Alternative Splicing; D000818:Animals; D001482:Base Composition; D018076:DNA, Complementary / Q000032:analysis; D005314:Embryonic and Fetal Development / Q000235:genetics; D005091:Exons / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009132:Muscles / Q000502:physiology*; D016254:Mutagenesis, Insertional; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics*
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta