title: Identification of immunological relevant phenotypes in ENU mutagenized mice.
abstract: The immunology screen focuses on the identification of novel gene products involved in the mammalian immune response and on the establishment of mouse models for immunological disorders. For this purpose, high throughput and semi-automated techniques were developed and optimized for low cost per sample and reproducibility. All assays are designed to be nonconsumptive and are based on peripheral blood or direct PCR amplification.
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Troger Strasse 4a, 81675 München, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Flaswinkel
authors.forename: F
authors.initials: F
authors.lastname: Alessandrini
authors.forename: B
authors.initials: B
authors.lastname: Rathkolb
authors.forename: T
authors.initials: T
authors.lastname: Decker
authors.forename: E
authors.initials: E
authors.lastname: Kremmer
authors.forename: A
authors.initials: A
authors.lastname: Servatius
authors.forename: T
authors.initials: T
authors.lastname: Jakob
authors.forename: D
authors.initials: D
authors.lastname: Soewarto
authors.forename: S
authors.initials: S
authors.lastname: Marschall
authors.forename: C
authors.initials: C
authors.lastname: Fella
authors.forename: H
authors.initials: H
authors.lastname: Behrendt
authors.forename: J
authors.initials: J
authors.lastname: Ring
authors.forename: E
authors.initials: E
authors.lastname: Wolf
authors.forename: R
authors.initials: R
authors.lastname: Balling
authors.forename: M
authors.initials: M
authors.lastname: Hrabé de Angelis
authors.forename: K
authors.initials: K
authors.lastname: Pfeffer
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005038:Ethylnitrosourea; D005434:Flow Cytometry; D007154:Immune System Diseases / Q000235:genetics*; D007109:Immunity / Q000235:genetics*; D007136:Immunoglobulins / Q000032:analysis; D020407:Internet; D051379:Mice / Q000235:genetics*; D016296:Mutagenesis; D009153:Mutagens; D010641:Phenotype; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D007136:Immunoglobulins; D009153:Mutagens; D005038:Ethylnitrosourea