[Baculovirus: an example of an insect virus of use to humans].
Baculovirus is a viral pathogen of insects in general and lepidoptera in particular. The genome of this large virus consists of a circular, infectious bicatenary DNA molecule. At the end of its replication cycle in insects, baculovirus produces a large quantity of at least two proteins, i.e., polyedrine and polypeptide P10. These proteins are essential for transmission of the virus in nature, but are not necessary in cell cultures. Using molecular recombinant techniques, one or both of the genes coding for these proteins can be replaced by heterologous genes. In this way, baculovirus raised in vitro can be used to produce large quantities of the alien proteins at the end of the multiplication cycle. So far more than 3,000 different proteins have been expressed including several presenting interest as diagnostic tools (Puumala virus, Herpes simplex virus) or therapeutic treatment in man and animals (vaccinations against dengue, flu, malaria and production of anti-Rhesus immunoglobulins). Since it is based on the use of lepidoptera virus, this system would appear to be particularly safe. No vertebrate virus is able to replicate in the cell system used. Use of this genetic engineering tool will undoubtedly expand and holds great promise for the future.
Devauchelle
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Institut National de Recherche Agronomique/CNRS, Saint Christol-lez-Alès, France. devauche@ensam.inra.fr
Cerutti
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M
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics / Q000502:physiology*; D004270:DNA, Circular / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D003715:Dengue / Q000517:prevention & control; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D006476:Orthohantavirus / Q000235:genetics; D006801:Humans; D007251:Influenza, Human / Q000517:prevention & control; D007313:Insecta / Q000821:virology; D007915:Lepidoptera / Q000821:virology; D008288:Malaria / Q000517:prevention & control; D011994:Recombinant Proteins / Q000096:biosynthesis*; D018139:Simplexvirus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines; D014779:Virus Replication / Q000235:genetics
D000888:Antibodies, Anti-Idiotypic; D004270:DNA, Circular; D004274:DNA, Recombinant; D004279:DNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C078072:p10 protein, Baculovirus