In vitro and ex vivo anti- and prooxidant components of Cichorium intybus.
The water soluble antioxidant properties of Cichorium intybus var. Silvestre, whose production zone is around Chioggia, Italy, were investigated. Vegetable juices were obtained by centrifugation, and (1) filtration at 2 degrees C; (2) filtration at 25 degrees C, and stored for 3 h; (3) boiled for 30 min at 102 degrees C, and then analysed. The antioxidant properties were evaluated in vitro as antioxidant activity (AA) (model system beta-carotene-linoleic acid) and ex vivo as protective activity (PA) against rat liver cell microsome lipid peroxidation measured as 2-thiobarbituric acid-reactive substances (TBA-RS) generated by peroxide degradation. All the vegetable juices showed high but very variable AA (> 83%) and PA (> 64%). After dialysis and analysis of fractions it was shown that the vegetable contained both biological antioxidant and prooxidant compounds. The prooxidants had MW < 3000, conversely the very active antioxidants (PA = 100%) had MW > 15,000. Electrophoretic analysis revealed that the most active fraction was a complex mixture of brown components at MW > 300,000.
Gazzani
G
G
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Pavia, Italy. gazzani@chifar.unipv.it
Daglia
M
M
Papetti
A
A
Gregotti
C
C
D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014675:Vegetables / Q000737:chemistry*
D000975:Antioxidants; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances