L929 cell conditioned medium protects RAW264.7 cells from oxidative injury through inducing antioxidant enzymes.
We have previously found that L929 cell conditioned medium (L929-CM) could protect mouse peritoneal macrophages from oxidative injury. To uncover the mechanism further, we investigated the effect of L929-CM on the oxidative injury caused by tbOOH to RAW264.7 cell lines. The results showed that L929-CM could protect RAW264.7 cells from oxidative injury (presented by cell morphology and cell survival rate), and L929-CM could also improve total superoxide dismutase (SOD), selenium-dependent and non-selenium-dependent glutathione peroxidase (SeGPx and non-SeGPx) activities in RAW264.7 cells. RT-PCR analysis showed that, L929-CM could induce plasma glutathione peroxidase (PLGPx) mRNA expression, while there was no inducing effect of L929-CM on phospholipid hydroperoxide glutathione peroxidase (PHGPx) mRNA expression in RAW264.7 cells. 5 microg/ml actinomycin D, 30 microg/ml cycloheximide (de novo protein synthesis inhibitor) and 50 microg/ml acetovanilone (intracellular superoxide anion production inhibitor) had no effects in attenuating the induction of PLGPx expression by L929-CM.
Pang
Z J
ZJ
Research Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou, People's Republic of China. zyjyx@fimmu.edu.cn
Chen
Y
Y
Zhou
M
M
D000818:Animals; D000975:Antioxidants; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned; D004790:Enzyme Induction; D015870:Gene Expression / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D012333:RNA, Messenger; D013482:Superoxide Dismutase / Q000096:biosynthesis* / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
D000975:Antioxidants; D017077:Culture Media, Conditioned; D016877:Oxidants; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase