O-(Acridinium)hydroxylamine (AHA): a reagent for the preparation of chemiluminescent acridinium oxime (AO)-steroid conjugates.
O-(Acridinium)hydroxylamine (AHA) reacted with a representative sample of oxo-steroids (6-oxoestradiol, estrone, norethindrone, cortisol, progesterone, digoxin dialdehyde, and digitoxin dialdehyde) to produce chemiluminescent acridinium oxime (AO) conjugates in a single step in 37-68% yield after preparative HPLC.
Adamczyk
M
M
Department of Chemistry, Diagnostics Division, Abbott Laboratories, Department 9NM, Bldg AP-20, 100 Abbott Park Road, Abbott Park, IL 60064-6016, USA. maciej.adamczyk@abbott.com
Grote
J
J
Mattingly
P G
PG
Pan
Y
Y
D000166:Acridines / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents / Q000737:chemistry*; D008163:Luminescent Measurements; D009682:Magnetic Resonance Spectroscopy; D010091:Oximes / Q000138:chemical synthesis* / Q000737:chemistry; D013256:Steroids / Q000138:chemical synthesis* / Q000737:chemistry
D000166:Acridines; D007202:Indicators and Reagents; D010091:Oximes; D013256:Steroids; C408992:acridinium hydroxylamine