[Effects of a few nutritional factors on content of coenzyme Q10 by tobacco plant cells in suspension culture].
With suspension cultured cells of tobacco (Nicoliana Tobacum L.), effects of inorganic phosphate, nitrogen sources and carbon source on cell growth and CoQ10 content were investigated. It showed that, When treated with 30 g/L of sucrose, the total amount of CoQ10 was highest (8212 g/L); and the cell biomass and CoQ10 content were 19.8 micrograms/L, 414.7 micrograms/g(dwt) respectively. When treated 340 mg/L of KH2PO4, the cell biomass, the content and total amount of CoQ10 were 21.04 g/L, 514.5 micrograms/g(dwt) and 10824 micrograms/L respectively. When treated with the ratio of NH4+/NO3- was 1:2, the cell biomass, the content and total amount of CoQ10 were 21.04 g/L, 514.3 micrograms/g(dwt) and 10,820 micrograms/L respectively. The high ration of NH4+/NO3- was suitable for the formation of CoQ10, but not for the cell growth.
Gao
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XY
College of Biotechnology, South China Agr. Univ., Guangzhou.
Ye
G H
GH
Mu
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H
Xu
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FC
D002455:Cell Division; D003067:Coenzymes; D009747:Nutritional Physiological Phenomena; D010710:Phosphates / Q000494:pharmacology; D010947:Plants, Toxic; D013535:Suspensions; D014026:Nicotiana / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis
D003067:Coenzymes; D010710:Phosphates; D013535:Suspensions; D014451:Ubiquinone; C024989:coenzyme Q10