Possible involvement of cholinergic and adrenergic mechanisms in changing contractility of guinea pig ileum by Ipomoea carnea.
The study aimed to elucidate the mechanism (s) of action of Ipomoea carnea leaf juice (ILJ) in changing contractility of guinea pig ileum. ILJ produced dose-dependent (10-10000 microg/ml) triphasic responses. The initial contractile phase was blocked by atropine (1 microg/ml) but had additive effect with acetylcholine (2 ng/ml) or carbachol (2 ng/ml). Neostigmine (30 ng/ml) or lignocaine (50 microg/ml) failed to alter the response. In cold-induced denervated preparations, this phase was augmented. The relaxatory phase of ILJ was not modified by phenoxybenzamine (35 microg/ml) but was reduced by propranolol (1 microg/ml) and abolished by lignocaine (50 microg/ml). The final contractile phase of ILJ was not affected by atropine (1 microg/ml). These results suggest that the triphasic response of ILJ is possibly mediated through cholinergic, adrenergic and non-cholinergic mechanisms, respectively.
Hore
S K
SK
Department of Pharmacology and Toxicology, College of Veterinary Science and A.H., I.G.A.U., Anjora, -491001 (MP), Durg, India.
Ottalwar
R
R
Koley
K M
KM
Pathak
A K
AK
D018663:Adrenergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects; D018678:Cholinergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D018663:Adrenergic Agents; D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D018678:Cholinergic Agents; D010936:Plant Extracts