Glucose deprivation does not affect GLUT1 targeting in 3T3-L1 adipocytes.
We have previously demonstrated that glucose deprivation alters the glycosylation of the GLUT1 glucose transporter in 3T3-L1 adipocytes. Many aberrantly glycosylated proteins are retained in the endoplasmic reticulum by interaction with chaperones. Herein, we use three independent procedures to show that GLUT1 is targeted to the plasma membrane, despite alterations in glycosylation. While earlier experiments revealed that plasma membrane targeting of aglyco GLUT 1 transporter was significantly reduced, our data show for the first time that altered glycosylation provides sufficient information to drive appropriate trafficking.
McMahon
R J
RJ
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Florida 32610, USA.
Hwang
J B
JB
Frost
S C
SC
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001710:Biotin / Q000378:metabolism; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D005947:Glucose / Q000494:pharmacology*; D051272:Glucose Transporter Type 1; D006031:Glycosylation; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*
D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495678:Slc2a1 protein, mouse; D001710:Biotin; D005947:Glucose