Use of low-temperature field emission scanning electron microscopy to examine mites.
Partly because mites are microscopic in size and fragile in nature, acarologists estimate that less than five percent of extant species have been taxonomically described. Recently, data from conventional scanning electron microscopy (SEM) have been used to facilitate the descriptions and complement the information that has been historically obtained with the light microscope. However, the conventional preparation techniques associated with SEM frequently prevent or compromise the results. This study evaluated the use of low-temperature field emission SEM to image mites and their hosts. Results indicated that a modified cryofixation procedure, which was associated with this technique, retained the mites at their living/feeding sites in natural behavioral positions. Furthermore, the turgor of the specimens, even eggs and soft-bodied species, was also maintained. The structure and orientation of delicate structures such as setae, which would be subjected to mechanical damage during conventional chemical fixation, dehydration, and drying, were also preserved after cryofixation. Field emission SEM, which provided useful magnification beyond that attainable with a conventional SEM, also enabled resolution of ultrastructural features, such as tenent hairs on the empodium and pores on the dorsal surface that had not previously been observed. These advantages indicate that the low-temperature field emission SEM can provide important structural data that can be used to study the anatomy, morphology, and bioecology of mites.
Wergin
W P
WP
Nematology Laboratory, USDA ARS, Beltsville Agricultural Research Center, Maryland 20705, USA.
Ochoa
R
R
Erbe
E F
EF
Craemer
C
C
Raina
A K
AK
D000818:Animals; D020285:Cryoelectron Microscopy; D015925:Cryopreservation; D007089:Image Enhancement; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008925:Mites / Q000033:anatomy & histology*