Effects of storage conditions and hemolysis on vitamin E concentrations in porcine serum and liver.
Vitamin E (alpha-tocopherol) is an antioxidant vitamin important in protecting unsaturated fatty acids in lipid membranes from peroxidation. Variation in collection, storage, and shipping conditions of samples can potentially lead to breakdown of vitamin E prior to analysis. Therefore, the purposes of this project were 1) to determine the stability of vitamin E in refrigerated and frozen porcine liver and serum and 2) to evaluate the effects of red blood cell (RBC) hemolysis on porcine serum vitamin E concentrations. Porcine liver and nonhemolyzed serum were collected and stored refrigerated or frozen. Samples were analyzed for vitamin E immediately or on days 2, 3, 7, or 14. In addition, porcine RBCs were added to normal serum at concentrations from 1 x 10(6) to 1 X 10(9) RBC/ml and hemolyzed by freeze-thaw prior to analysis for vitamin E or products of lipid peroxidation.
Hooser
S B
SB
Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN 47907, USA.
McCarthy
J M
JM
Wilson
C R
CR
Harms
J L
JL
Stevenson
G
G
Everson
R J
RJ
D000818:Animals; D004912:Erythrocytes; D006403:Hematologic Tests / Q000662:veterinary; D006461:Hemolysis; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry*; D013048:Specimen Handling; D013552:Swine / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism / Q000493:pharmacokinetics*
D014810:Vitamin E