Identification of major histocompatibility complex class II-associated peptides derived from freshly prepared rat Langerhans cells using MALDI-PSD and Edman degradation.
The isolation and identification is described of MHC class II-bound peptides derived from Langerhans cells. A combination of preparative micro-HPLC, MALDI-MS, Edman degradation was used for determining the amino acid sequence of MHC-associated peptides. Sample handling was crucial because fractions containing trace amounts of material require immediate storage at -80 degrees C to prevent peptide losses.
Sickmann
A
A
Institut für Physiologische Chemie, Ruhr-Universität Bochum, Germany.
Blüggel
M
M
Kulke
M
M
Kremmin
H
H
Marx
A
A
Reske
K
K
Meyer
H E
HE
D000818:Animals; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007801:Langerhans Cells / Q000276:immunology*; D013058:Mass Spectrometry / Q000379:methods; D051381:Rats; D011917:Rats, Inbred Lew; D012867:Skin / Q000276:immunology*
D000949:Histocompatibility Antigens Class II