Mutational analysis of the putative receptor-binding domain of Moloney murine leukemia virus glycoprotein gp70.
The entry of Moloney murine leukemia virus (MoMuLV) to murine cells is mediated by the binding of its envelope glycoprotein gp70 to its receptor, the cationic amino acid transporter MCAT-1. The binding property of the envelope protein lies mainly in the N-terminal half of the protein. To identify essential residues involved in the binding of gp70 to its receptor, we have mutated amino acids within the putative receptor-binding domain of MoMuLV gp70. Changes in the residues P94 and W100 resulted in lower viral titers in comparison to the wild-type virions. Single, double, or triple point mutations involving the residue W100 make the envelope protein severely defective in binding to its receptor. Binding studies and cell fusion experiments with murine XC cells suggested that the residue W100 might play an important role in the process of infection by making contact between gp70 and its receptor.
Panda
B R
BR
Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, United Kingdom.
Kingsman
S M
SM
Kingsman
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002459:Cell Fusion; D002460:Cell Line; D005434:Flow Cytometry; D015870:Gene Expression; D015726:Giant Cells / Q000166:cytology / Q000378:metabolism / Q000821:virology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009154:Mutation / Q000235:genetics*; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D015682:Retroviridae Proteins, Oncogenic / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015163:Superinfection; D014162:Transfection; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; C070506:ecotropic murine leukemia virus receptor