[Specific humoral response in Balb/c mice inoculated with a genome library of expression of Trypanosoma cruzi].
A genomic expression library of Trypanosoma cruzi (T. cruzi) was made using plasmid pcDNA3 as a vector, with which male mice from the Balb/c isogenic line were intramuscullary inoculated. It was used a positive control group that was administered soluble antigens of T. cruzi. Other 2 groups received genomic and plasmid DNA, respectively. One group was not immunized. Weekly blood samples were obtained from all the animals until the fourth week and 2 weeks after reimmunization to study the response of specific antibodies against the microorganism antigens by an indirect immunoenzymatic assay (ELISA). It was observed a significant increase of specific antibodies in the animals reimmunized with 50 micrograms of the library, as well as in the group immunized with soluble antigens of T. cruzi.
Amador
E A
EA
ciipk@infomed.sld.cu
Acosta Domínguez
A
A
Sarmiento
M E
ME
Hidalgo Grass
C
C
Fachado Carvajales
A
A
Vidal Martínez
T
T
Leyva
J L
JL
Fonte Galindo
L
L
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000235:genetics*; D016054:DNA, Protozoan / Q000008:administration & dosage / Q000235:genetics*; D015698:Genomic Library; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014349:Trypanosoma cruzi / Q000235:genetics*
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007074:Immunoglobulin G