[Expression and characterization of HIV-1 Gag p17-p24 protein].
The biological and immunological characteristics of HIV-1 core protein p17-24 expressed by recombinant vaccinia viruses were studied. The results of indirect immunofluorescence assay (I-IFA), Western blot and dot ELISA showed that the two recombinants could express the p24 gag and p17-24 gag fusion proteins in infected cell lines respectively. The electromicroscope observation revealed that the expressed proteins could also assemble into virus-like particles. The recombinant vaccinia viruses can also stimulate mice for the formation of anti HIV-1 Gag p24 antibody. When infected with the recombinant viruses, the chromosome DNA ladder caused by the apoptosis of the BHK cell was observed.
Li
T Y
TY
Changchun University of Agriculture and Animal Sciences.
Jin
N Y
NY
Wang
H W
HW
Guo
Z R
ZR
Fang
H H
HH
An
R G
RG
Yin
Z
Z
D000818:Animals; D015683:Gene Products, gag / Q000096:biosynthesis*; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000096:biosynthesis*; D016655:HIV Core Protein p24 / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1