Cloning of cDNA encoding ethylene-responsive element binding protein-5 in the cultured cells of Nicotiana tabacum.
We have isolated a full-length cDNA clone from the cultured cells of Nicotiana tabacum, of which the deduced 225 amino acid sequence showed significant homology to the known ethylene-responsive element binding proteins (EREBPs) from tobacco and Arabidopsis. Although the clone shared substantial homology with an Arabidopsis ethylene-responsive element binding factor-3 (AtERF-3) throughout the full length, such a clone had not been found in N. tabacum cells yet; the clone was found to be 5th homologue of EREBPs on N. tabacum.
Ashida
Y
Y
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan.
Yokobatake
N
N
Kohchi
C
C
Shimoda
K
K
Hirata
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000235:genetics*; D010940:Plant Proteins; D010947:Plants, Toxic; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000166:cytology / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C093680:ethylene-responsive element binding protein