Silanized nucleic acids: a general platform for DNA immobilization.
We have developed a method for simultaneous deposition and covalent cross-linking of oligonucleotide or PCR products on unmodified glass surfaces. By covalently conjugating an active silyl moiety onto oligonucleotides or cDNA in solutions we have generated a new class of modified nucleic acids, namely silanized nucleic acids. Such silanized molecules can be immobilized instantly onto glass surfaces after manual or automated deposition. This method provides a simple and rapid, yet very efficient, solution to the immobilization of prefabricated oligonucleotides and DNA for chip production.
Kumar
A
A
Genomics Technology Unit, Center for Genomics Research, Karolinska Institutet, Theorells vägen 3, 17177, Stockholm, Sweden.
Larsson
O
O
Parodi
D
D
Liang
Z
Z
D003432:Cross-Linking Reagents; D018076:DNA, Complementary / Q000737:chemistry* / Q000302:isolation & purification; D005898:Glass; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D017646:Organosilicon Compounds; D016133:Polymerase Chain Reaction / Q000379:methods*; D012821:Silanes / Q000737:chemistry*
D003432:Cross-Linking Reagents; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D017646:Organosilicon Compounds; D012821:Silanes; C102833:(3-mercaptopropyl)trimethoxysilane