Persistent increase in the amount of aquaporin-5 in the apical plasma membrane of rat parotid acinar cells induced by a muscarinic agonist SNI-2011.
SNI-2011 induces the long-lasting increase in the amount of aquaporin-5 (AQP5) in apical plasma membranes (APMs) of rat parotid acini in a concentration-dependent manner. This induction was inhibited by p-F-HHSiD, U73122, TMB-8, or dantrolene but not by bisindolmaleimide or H-7, indicating that SNI-2011 acting at M(3) muscarinic receptors induced translocation of AQP5 via [Ca(2+)](i) elevation but not via the activation of protein kinase C. In contrast, acetylcholine induced a transient translocation of AQP5 to APMs. SNI-2011 induces long-lasting oscillations of [Ca(2+)](i) in the presence of extracellular Ca(2+). Thus, SNI-2011 induces a long-lasting translocation of AQP5 to APMs coupled with persistent [Ca(2+)](i) oscillations.
Ishikawa
Y
Y
Department of Pharmacology, School of Dentistry, Tokushima University, 3-18-15 Kuramoto-cho, 770-8504, Tokushima, Japan. isikawa@dent.tokushima-u.ac.jp
Skowronski
M T
MT
Ishida
H
H
D000818:Animals; D051402:Aquaporin 5; D020346:Aquaporins / Q000378:metabolism*; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D018721:Muscarinic Agonists / Q000494:pharmacology*; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D011812:Quinuclidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013876:Thiophenes
C496040:Aqp5 protein, rat; D051402:Aquaporin 5; D020346:Aquaporins; D008565:Membrane Proteins; D018721:Muscarinic Agonists; D010880:Piperidines; D011812:Quinuclidines; D013876:Thiophenes; D001285:Atropine; D015544:Inositol 1,4,5-Trisphosphate; C059240:cevimeline; C046773:hexahydrosiladifenidol; D002118:Calcium