title: Identification and characterization of a novel gene, hos3+, the function of which is necessary for growth under high osmotic stress in fission yeast.
abstract: hos3 mutants of the fission yeast Schizosaccharomyces pombe showed the phenotype of high osmolarity sensitivity for growth. An S. pombe strain carrying the hos3-M26 allele cannot form colonies on agar plates containing 2 M glucose, but the parental strain can do so very well, as demonstrated previously. The hos3+ gene was cloned and identified as one that encodes a small protein of 94 amino acids, which shows no sequence similarity to any other proteins in the current databases. A hos3delta strain, which we then constructed, had the phenotype of high osmolarity sensitivity, as in the case of the original hos3-M26 mutant. More interestingly, when these hos- cells were grown in the non-permissive growth condition in the presence of 2 M glucose, we found that unusually many septated cells were accumulated after a prolonged incubation. A multicopy suppressor gene for hos- mutations was also isolated and identified as the dsk1+ gene encoding a protein kinase, which was previously suggested to be implicated in a process of the mitotic regulation of S. pombe. The function of the hos3+ gene is discussed from these results.
authors.affiliation: Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Aoyama
authors.forename: R
authors.initials: R
authors.lastname: Kawaura
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Aiba
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
mesh_terms: D003001:Cloning, Molecular; D009154:Mutation; D009994:Osmolar Concentration; D010957:Plasmids; D011994:Recombinant Proteins; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development*
chemical_list: D011994:Recombinant Proteins