Two pheromone precursor genes are transcriptionally expressed in the homothallic ascomycete Sordaria macrospora.
In order to analyze the involvement of pheromones in cell recognition and mating in a homothallic fungus, two putative pheromone precursor genes, named ppg1 and ppg2, were isolated from a genomic library of Sordaria macrospora. The ppg1 gene is predicted to encode a precursor pheromone that is processed by a Kex2-like protease to yield a pheromone that is structurally similar to the alpha-factor of the yeast Saccharomyces cerevisiae. The ppg2 gene encodes a 24-amino-acid polypeptide that contains a putative farnesylated and carboxy methylated C-terminal cysteine residue. The sequences of the predicted pheromones display strong structural similarity to those encoded by putative pheromones of heterothallic filamentous ascomycetes. Both genes are expressed during the life cycle of S. macrospora. This is the first description of pheromone precursor genes encoded by a homothallic fungus. Southern-hybridization experiments indicated that ppg1 and ppg2 homologues are also present in other homothallic ascomycetes.
Pöggeler
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Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Germany. Stefanie.Poeggeler@ruhr-uni-bochum.de
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D005656:Fungal Proteins; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010675:Pheromones / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012098:Reproduction; D020037:Sordariales / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
D005656:Fungal Proteins; D010675:Pheromones; C415591:Ppg1 protein, Sordaria macrospora; C415592:Ppg2 protein, Sordaria macrospora; D011498:Protein Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger