Changes in free and conjugated polyamines during starvation of sugarcane juices as analyzed by high-performance liquid chromatography.
Changes of the polyamines (PAs) titer in high- and mid-molecular-mass carbohydrates (HMMCs and MMMCs, respectively) obtained from sugarcane juices stored for 72 h at pH 5.2 or clarified at pH 8.0 have been studied. Cadaverine (CAD) is the most abundant free (S) PA in the MMMC fraction from juices at pH 5.2, whereas putrescine (PUT) was revealed as the main PA at pH 8.0. A slight increase in the free PUT titer can be noted at pH 5.2 for 72 h of juice starvation. PAs from MMMC were mainly conjugated to acid-insoluble (PH) molecules. Accumulation of PH-PAs with the time of starvation was especially significant for PUT and CAD. However, CAD has also been detected in the acid-soluble (SH) fraction and its concentration increases with the time of starvation at pH 5.2. The accumulation pattern of free and conjugated PAs from HMMCs is similar to that found for MMMCs although some differences can be observed. For instance, the increase in free PUT with the time at pH 8.0 was 2.7-times higher in the HMMC fraction than in the MMMC fraction. Conjugated PAs associated to acid-soluble macromolecules (SH fraction) achieved a level in HMMC fractions higher than that observed in the MMMC fraction. Moreover, the reported increase with time that was observed in PH-CAD from the MMMC fraction was not observed in the HMMC fraction, and, finally, the increase in PH-PUT with the time was lower for the HMMC fraction than for the MMMC fraction.
Rodríguez
C W
CW
Department of Plant Physiology, Faculty of Biology, Havana University, Cuba.
de Armas
R
R
Vicente
C
C
Legaz
M E
ME
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010944:Plants / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*
D011073:Polyamines