Convergent synthesis of ribonuclease L-active 2',5'-oligoadenylate-peptide nucleic acids.
2-5A was conjugated to N-(2-aminoethyl)-glycyl PNA by periodate oxidization, followed by coupling with amino-derivatized PNA and final cyanoborohydride reduction. An adduct of 2-5A pentamer with tetrameric thymine PNA activated RNase L with the same potency as earlier versions of 2-5A-PNA or 2-5A-DNA.
Wang
Z
Z
Section on Biomedical Chemistry, Laboratory of Medicinal Chemistry, National Institute of Diabetes and Digestive antd Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0805, USA.
Chen
L
L
Bayly
S F
SF
Torrence
P F
PF
D000227:Adenine Nucleotides / Q000737:chemistry*; D004722:Endoribonucleases / Q000378:metabolism*; D009843:Oligoribonucleotides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D020135:Peptide Nucleic Acids; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease