title: Identification of a point mutation in an esterase gene in different populations of the southern cattle tick, Boophilus microplus.
abstract: Two esterase cDNA sequences were obtained from susceptible and organophosphorus resistant strains of Boophilus microplus. Both sequences have a high degree of homology to carboxylesterase B. One gene has identical sequences in both strains and the other showed two point mutations. One mutation produces an amino acid substitution when the amino acid sequence is deduced, this mutation was detected in six different populations susceptible and resistant to insecticides, but a pyrethroid resistant strain was the only one that showed only the mutant allele. Identification of this mutation and the strong signal detected in southern blot with this strain, suggest that esterases are contributing to detoxification of pyrethroid compounds, as a resistant mechanism in Mexican strains of the southern cattle tick.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
authors.forename: R
authors.initials: R
authors.lastname: Hernandez
authors.forename: H
authors.initials: H
authors.lastname: He
authors.forename: A C
authors.initials: AC
authors.lastname: Chen
authors.forename: S D
authors.initials: SD
authors.lastname: Waghela
authors.forename: G
authors.initials: G
authors.lastname: Wayne Ivie
authors.forename: J E
authors.initials: JE
authors.lastname: George
authors.forename: G
authors.initials: G
authors.lastname: Gale Wagner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary; D004950:Esterases / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D017386:Sequence Homology, Amino Acid; D013987:Ticks / Q000201:enzymology* / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004950:Esterases