Cytogenetic effect of the anticancer drug epirubicin on Chinese hamster cell line in vitro.
The present study demonstrated the cytogenetic effect of the anticancer drug epirubicin on cultures of Chinese hamster cell line in vitro. The cultures were exposed to the drug for 24 h at three final concentrations; 10, 20 and 40 microg/ml. All treatments were carried out in the absence of any exogenous metabolic activation system. The different types of structural chromosomal aberrations, including gaps, breaks, deletions and fragments were increased in epirubicin-treated cultures. This increase was dose dependent where there was a positive correlation between increased drug concentration and induction of structural chromosomal aberrations. Also, the numerical chromosomal aberrations, including hypodiploidy and hyperdiploidy, were increased significantly in epirubicin-treated cultures. Like structural aberrations, the increase of numerical chromosomal aberrations was also dose-dependent. The frequency of sister chromatid exchanges in cultures treated with epirubicin increased significantly and this increase was dose-dependent. On the other hand, the epirubicin significantly decreased the mitotic index in treated cultures of Chinese hamster cell line.
El-Mahdy Sayed Othman
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Cell Biology Department, National Research Center, Dokki, Giza, Cairo, Egypt.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001711:Biotransformation; D016466:CHO Cells / Q000187:drug effects*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D006224:Cricetinae; D003412:Cricetulus; D015251:Epirubicin / Q000633:toxicity*; D008940:Mitotic Index; D015394:Molecular Structure; D012854:Sister Chromatid Exchange / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D015251:Epirubicin