Effect of lead on Sertoli-germ cell coculture of rat.
Mixed cultures of Sertoli and germ cells were prepared from rat testes and their response to lead (Pb) was studied. Cultures consisted of a monolayer of Sertoli cells to which clusters of germ cells were attached. The effect of Pb added as lead acetate was tested at 0.0, 0.4, 4.0 and 40.0 microM for 24 and 48 h intervals. Addition of Pb to the culture medium caused germ cells to progressively detach from the Sertoli cell monolayer into the medium in a concentration and duration dependent manner Viability of the detached cells as judged by trypan blue exclusion test showed a decrease with increase in time and concentration of Pb. Significant leakage of lactate dehydrogenase (LDH) was recorded in the culture media only at the higher concentrations of 4.0 and 40.0 microM. Thus Pb at the doses tested induced cytotoxicity in rat Sertoli-germ cell coculture.
Adhikari
N
N
Embryotoxicology Division, Industrial Toxicology Research Center, Mahatma Gandhi Marg, Lucknow, India.
Sinha
N
N
Saxena
D K
DK
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D018920:Coculture Techniques; D004305:Dose-Response Relationship, Drug; D005854:Germ Cells / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007854:Lead / Q000633:toxicity*; D008297:Male; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects* / Q000473:pathology
D007854:Lead; D007770:L-Lactate Dehydrogenase