Transcriptional analysis of major heat shock genes of Helicobacter pylori.
The transcriptional organization and heat inducibility of the major heat shock genes hrcA, dnaK, dnaJ, groEL, and htpG were analyzed on the transcriptional level in Helicobacter pylori strain 69A. The strongly heat-induced dnaK operon was found to be tricistronic, consisting of the genes hrcA, grpE, and dnaK. The dnaJ gene specified one monocistronic mRNA which was also heat inducible. The genes groES and groEL were transcribed as one strongly heat-inducible bicistronic mRNA which exhibited exactly the same induction kinetic as the dnaK operon. Surprisingly, transcription of the monocistronic htpG gene was switched off after heat shock. The data presented are discussed with regard to the different mechanisms regulating expression of heat shock genes in H. pylori
Homuth
G
G
Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany.
Domm
S
S
Kleiner
D
D
Schumann
W
W
D000251:Adenosine Triphosphatases / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D018833:Chaperonins / Q000235:genetics; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D016480:Helicobacter pylori / Q000235:genetics*; D006358:Hot Temperature; D018832:Molecular Chaperones / Q000235:genetics; D009876:Operon; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; C097970:GroESL protein, Bacteria; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger; C066720:HtpG protein, bacteria; D000251:Adenosine Triphosphatases; D018833:Chaperonins; C063842:dnaK protein, E coli