[The comparison of sensitivity between immunostaining and a simplified PCR-cold SSCP method in p53 genomic mutations].
The PCR-SSCP (single strand conformational polymorphism) method has been widely employed to screen mutations in a variety of genes because of its rapidity and simplicity in the operation. Using this method, we have examined mutations of some tumor-related genes including p53 and Ki-ras. In this study, we have evaluated the PCR-Cold (non radioactive) SSCP method for detection of p53 point mutations in comparison with immunohistological detection of p53 and PCR-direct sequencing. The results indicated that the PCR-Cold SSCP method had the same sensitivity with that of PCR-direct sequencing method, and had higher sensitivity than that of immunohistochemical method (IHC).
Ueno
I
I
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto.
Ota
H
H
Hidaka
E
E
Ishikawa
M
M
Hirota
M
M
Nara
K
K
Katsuyama
T
T
D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D019656:Loss of Heterozygosity; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000235:genetics