The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA.
F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 microg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this method economically attractive.
Barbosa
G H
GH
Laboratório de Imunopatologia Keizo Asami, Brasil.
Santana
E M
EM
Almeida
A M
AM
Araujo
A M
AM
Fatibello-Filho
O
O
Carvalho
L B
LB
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000295:instrumentation*; D005374:Filtration / Q000295:instrumentation; D006041:Goats; D010930:Plague / Q000276:immunology*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D011817:Rabbits; D015010:Yersinia pestis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011142:Polyvinyl Alcohol