title: Polyphasic identification of wild yeast strains isolated from Greek sourdoughs.
abstract: A total of forty-five wild yeast strains were isolated from five traditional Greek wheat sourdoughs. Strains were identified using the classical identification technique along with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole cell proteins (SDS-PAGE), Fourier transform-infrared spectroscopy (FT-IR) and the randomly amplified polymorphic DNA-polymerase chain reaction analysis (RAPD-PCR). The latter methods confirmed the classical identification. According to the results obtained, fourteen strains were identified as Saccharomyces cerevisiae strains, twenty-five as Pichia membranaefaciens strains and six as Yarrowia lipolytica.
authors.affiliation: Department of Food Science and Technology, Agricultural University of Athens, Greece. pspiros@mailcity.com
authors.forename: S
authors.initials: S
authors.lastname: Paramithiotis
authors.lastname: Müller MRA
authors.forename: M A
authors.initials: MA
authors.lastname: Ehrmann
authors.forename: E
authors.initials: E
authors.lastname: Tsakalidou
authors.forename: H
authors.initials: H
authors.lastname: Seiler
authors.forename: R
authors.initials: R
authors.lastname: Vogel
authors.forename: G
authors.initials: G
authors.lastname: Kalantzopoulos
mesh_terms: D001939:Bread / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006115:Greece; D006863:Hydrogen-Ion Concentration; D010843:Pichia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal