Evaluation of a water-extracted pharmamedia agar medium for the culture of fungi.
The purpose of this investigation was to determine the value of a water-extracted Pharmamedia agar medium for the culture of fungi. The growth of the fungi on this medium was compared to that on Sabouraud brain heart infusion agar. The Pharmamedia extract agar supported the growth of the 42 filamentous and yeast or yeast-like fungi that were examined. The medium was relatively clear to easily permit observation of the diagnostic microscopic and macroscopic architecture of the organisms. The Pharmamedia extract agar also permitted the conversion of the mold-to-yeast phase with Blastomyces dermatitides and Penicillium marneffei. This investigation supports the use of Pharmamedia as a culture medium for the clinical microbiology laboratory. Its relative simplicity of preparation and effective use as a culture medium, as well as a conversion medium, emphasizes its useful application for clinical mycology.
Slifkin
M
M
Microbiology Section, Department of Pathology and Laboratory Medicine, Allegheny General Hospital, 320 E. North Avenue, Pittsburgh, PA 15212, USA.
D000362:Agar; D001431:Bacteriological Techniques; D001758:Blastomyces / Q000254:growth & development; D003470:Culture Media / Q000032:analysis; D005069:Evaluation Studies as Topic; D005658:Fungi / Q000254:growth & development*; D006658:Histoplasma / Q000254:growth & development; D010407:Penicillium / Q000254:growth & development
D003470:Culture Media; D000362:Agar