title: Polymorphisms in the HLA-linked olfactory receptor genes in the Hutterites.
abstract: Genes in the MHC have been associated with mate choice and odor preferences in a variety of animals. Although the role of HLA genes in human mate choice has been controversial, studies in the Hutterites have demonstrated fewer than expected numbers of couples who match for an HLA haplotype, suggesting that in this population there is avoidance of mates with HLA haplotypes similar to one's own haplotype. Recently, 18 olfactory receptor (OR) genes have been mapped to the HLA region, telomeric to the HLA-F locus, providing a potential mechanism for HLA-based odor recognition and perhaps mate preferences in humans. We screened a sample of Hutterites with diverse HLA haplotypes for polymorphisms in the HLA-linked olfactory receptor gene, FAT11, by sequencing, denaturing high performance liquid chromatography, and allele-specific oligo dot-blotting. Three single nucleotide polymorphisms were detected in the single translated exon of this gene, all of which resulted in amino acid substitutions (Phe587Leu, Ala642Val, and Thr1157Ala). The FAT11 Phe587- Val642-Ala1157 allele occurred on 17 different HLA haplotypes, the Leu587-Ala642-Ala1157 allele on 15 haplotypes, the Phe587-Ala642-Ala1157 allele on 16 haplotypes, and the Phe587-Ala642-Thr1157 allele on a single haplotype. Thus, four alleles of the FAT11 gene are present in the Hutterites. This level of variation in the FAT11 gene alone may not be sufficient to contribute to the observed patterns of mate choice in the Hutterites and to individual variation in odor preferences.
authors.affiliation: Department of Human Genetics, University of Chicago, IL 60637, USA.
authors.forename: A C
authors.initials: AC
authors.lastname: Eklund
authors.forename: M M
authors.initials: MM
authors.lastname: Belchak
authors.forename: K
authors.initials: K
authors.lastname: Lapidos
authors.forename: R
authors.initials: R
authors.lastname: Raha-Chowdhury
authors.forename: C
authors.initials: C
authors.lastname: Ober
mesh_terms: D000483:Alleles; D002835:Christianity; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005006:Ethnicity / Q000235:genetics; D015234:HLA-A Antigens / Q000276:immunology*; D006801:Humans; D008285:Major Histocompatibility Complex; D008565:Membrane Proteins; D011110:Polymorphism, Genetic; D011956:Receptors, Cell Surface / Q000235:genetics*; D018035:Receptors, Odorant / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D015234:HLA-A Antigens; D008565:Membrane Proteins; C095526:OR2H2 protein, human; D011956:Receptors, Cell Surface; D018035:Receptors, Odorant