Selecting anti-HBsAg fab phage antibodies from the phage antibody library.
OBJECTIVE
Selecting Fab phage antibodies to HBsAg from the phage antibody library.


METHODS
The phages displaying antibody fragments were subjected to three rounds of panning with HBsAg coated in solid phase.


RESULTS
The eluted phages were enriched nearly 80-fold, and the percentage of recombinant clones increased from 17% to 100% after the third round of panning. The HBsAg specific monoclonal antibodies were determined by ELISA. These antibodies combined specifically with HBsAg, but not with BSA and HAV antigens.


CONCLUSIONS
Our work on constructing human immunoglobulin combinatorial library and selecting anti-HBsAg Fab phage antibodies would be beneficial to the further study on the production of soluble Fab antibodies.
Wang
Z
Z
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Liu
Q
Q
Wan
Z
Z
Zhang
D
D
D001435:Bacteriophages / Q000235:genetics*; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis*; D019151:Peptide Library
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library