Antigenic properties and diagnostic potential of baculovirus-expressed infectious bursal disease virus proteins VPX and VP3.
The routine technique for detecting antibodies specific to infectious bursal disease virus (IBDV) is a serological evaluation by enzyme-linked immunosorbent assay (ELISA) with preparations of whole virions as the antigens. To avoid using complete virus in the standard technique, we have developed two new antigens through the expression of the VPX and VP3 genes in insect cells. VPX and especially VP3 were expressed at high levels in insect cells and simple to purify. The immunogenicity of both proteins was similar to that of the native virus. VPX was able to elicit neutralizing antibodies but VP3 was not. Purified VPX and VP3 were tested in an indirect ELISA with more than 300 chicken sera. There was an excellent correlation between the results of the ELISA using VPX and those of the two commercial kits. VP3 did not perform as well as VPX, and the linear correlation was significantly lower. A comparison with the standard reference technique, seroneutralization, showed that the indirect ELISA was more sensitive. Therefore, VPX-based ELISA is a good alternative to conventional ELISAs that use whole virions.
Martínez-Torrecuadrada
J L
JL
INGENASA, 28037 Madrid, Spain.
Lázaro
B
B
Rodriguez
J F
JF
Casal
J I
JI
D000818:Animals; D016367:Baculoviridae; D018175:Birnaviridae Infections / Q000175:diagnosis* / Q000276:immunology; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000276:immunology*
D036022:Capsid Proteins; D011994:Recombinant Proteins; D054334:Viral Regulatory and Accessory Proteins