Study of cellular responses to polymeric biomaterials using the differential display method.
In this study, we attempted to detect altered gene expressions in the cells that had adhered to various surfaces using the differential display method. Thioglycollate-elicited peritoneal exudate cells (PEC) and mouse fibroblast (L929) cells were cultured on the polymer films. After a predetermined time, the total RNA was isolated from cells and the differential mRNA expressions were evaluated by RT-PCR method. As a result, in the differential display of amplified cDNA from PEC, the different patterns of cDNA fragments among the samples were obtained. This indicates that there were many different mRNA expressions depending on the polymer surfaces. The use of differential method was proven to be useful for studying cell-polymer interaction.
Kato
S
S
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Korimoto, Japan.
Kishida
A
A
Hanyu
N
N
Maruyama
I
I
Akashi
M
M
D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005466:Fluorocarbons / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009757:Nylons / Q000494:pharmacology; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects; D020959:Polyethylene / Q000494:pharmacology; D011137:Polystyrenes / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012828:Silicones / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D001672:Biocompatible Materials; D018076:DNA, Complementary; D005466:Fluorocarbons; D009757:Nylons; D011137:Polystyrenes; D012333:RNA, Messenger; D012828:Silicones; D020959:Polyethylene; C015531:tetrafluoroethylene