title: Dexamethasone modifies the effect of Pseudomonas aeruginosa exotoxin A on hearing.
abstract: In the present study, the protective effect of dexamethasone was analysed following exposure of the cochlea to Pseudomonas aeruginosa Exotoxin A (PaExoA). Four groups of albino Sprague-Dawley rats were used. 20 microl saline was instilled through the tympanic membrane into the round window niche (group A, n = 4); 1 microg/20 microl dexamethasone sodium 21-phosphate (dexamethasone) solution was instilled (group B, n = 4); 1 microg/20 microl PaExoA solution was initially instilled followed 1 h later by 20 microl saline (group C, n = 6); and 1 microg/20 microl PaExoA solution was initially instilled followed 1 h later by 1 microg/20 microl dexamethasone solution (group D, n = 6). Frequency-specific (4, 8, 10, 12, 16 and 20 kHz) auditory brainstem responses (ABR) were used to ascertain the threshold prior to exposure and 1, 2, 3, and 5 days and 1 and 2 weeks afterwards. No threshold change was observed in groups A and B, whereas the animals in groups C and D showed some threshold elevation, that in D being smaller than that in C. There was a significant difference at the frequencies 12, 16 and 20 kHz, 2 and 5 days after exposure. The intensity-latency (I-L) curve showed that in group D the cochlear component almost disappeared at high frequency one week after exposure. Our results indicate that dexamethasone can modify the effect of PaExoA caused by non-specific inflammation.
authors.affiliation: Department of Otorhinolaryngology and Head & Neck Surgery, Uppsala University Hospital, Sweden. naonobu.takeuchi@ent.uu.se
authors.forename: N
authors.initials: N
authors.lastname: Takeuchi
authors.forename: M
authors.initials: M
authors.lastname: Anniko
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003051:Cochlea / Q000187:drug effects / Q000382:microbiology; D003907:Dexamethasone / Q000494:pharmacology*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D005098:Exotoxins / Q000009:adverse effects* / Q000378:metabolism*; D006316:Hearing Loss, High-Frequency / Q000517:prevention & control; D006319:Hearing Loss, Sensorineural / Q000517:prevention & control; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014432:Tympanic Membrane / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005098:Exotoxins; D003907:Dexamethasone