title: Great amount of C.pneumoniae in ruptured plaque vessel segments at autopsy. A comparative study with stable plaques.
abstract: A possible relationship between C.pneumoniae (CP) infection, atherosclerosis and acute myocardial infarction is a debated matter. Now we performed the search of CP in histological segments of fatal ruptured plaques and of stable plaques by histochemistry (Macchiavello stain), immunohistochemistry and in situ hybridization techniques. Electron microscopy and confocal laser microscopy techniques were used in two additional cases. The semi-quantification of CP + cells (0-4+) and quantification of lymphocytes demonstrated greater amount of CP + cells and more inflammation in the adventitia of vulnerable plaque vessel segments than of stable ones, larger amount of CP + cells in adventitia than in the plaque and high frequency of CP + cells in all groups studied. This preliminary study strongly suggests a direct pathogenetic involvement of adventitial CP in the rupture of the atheromatous plaque, development of acute myocardial infarction and also in the development of atherosclerosis.
authors.affiliation: Heart Institute, Medical School, University of São Paulo, São Paulo, SP, Brazil. anplourdes@incor.usp.br
authors.forename: M d
authors.initials: Md
authors.lastname: Higuchi
authors.forename: J B
authors.initials: JB
authors.lastname: Castelli
authors.forename: V D
authors.initials: VD
authors.lastname: Aiello
authors.forename: S
authors.initials: S
authors.lastname: Palomino
authors.forename: M M
authors.initials: MM
authors.lastname: Reis
authors.forename: N V
authors.initials: NV
authors.lastname: Sambiase
authors.forename: S
authors.initials: S
authors.lastname: Fukasawa
authors.forename: H G
authors.initials: HG
authors.lastname: Bezerra
authors.forename: J A
authors.initials: JA
authors.lastname: Ramires
mesh_terms: D002690:Chlamydia Infections / Q000150:complications*; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D003324:Coronary Artery Disease / Q000382:microbiology*; D006801:Humans; D009203:Myocardial Infarction / Q000382:microbiology; D012189:Retrospective Studies; D012421:Rupture