Genome size variation in Arachis hypogaea and A. monticola re-evaluated.
Genome size variation within species is a frequently reported, but still a controversial problem. In the present study, we re-evaluated recently published Feulgen densitometric data on genome size and its infraspecific variation in Arachis hypogaea, and also conducted measurements in one accession of its wild relative A. monticola. The methods applied were propidium iodide flow cytometry and Feulgen densitometry using Pisum sativum as an internal standard. The 2C DNA contents previously published cannot be confirmed, but values obtained in this study are about half as large. Additionally, we could not reproduce the previously reported 1.15-fold variation within A. hypogaea; our data indicate genome size stability between respective accessions of this species. Based on 8.84 pg (2C) for Pisum sativum the DNA amounts (2C) were: 5.914 pg in A. hypogaea, and 5.979 pg in A. monticola.
Temsch
E M
EM
Institute of Botany of the University of Vienna, Austria. temsch@s1.botanik.univie.ac.at
Greilhuber
J
J
D010367:Arachis / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis; D003720:Densitometry; D005434:Flow Cytometry; D014644:Genetic Variation; D018745:Genome, Plant; D012639:Seeds / Q000235:genetics
D018744:DNA, Plant