Optimization of a capillary electrophoresis-electrospray mass spectrometry method for the quantitation of the 20 natural amino acids in childrens blood.
This paper describes useful information on the capillary electrophoresis-electrospray ionization mass spectroscopy (CE-ESIMS) interfacing for the analysis of amino acids (AAs) in standard mixtures and in child plasma blood serum. The developed procedure allows quantitation of the 20 natural AAs, in a single run, without any derivatization. Limits of detection as low as 3-20 micromol/l (5-30 pg injected) per analyte were obtained with an efficiency of about 100,000 plates and a peak area relative standard deviation below 4%.
Martin-Girardeau
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A
Laboratoire des Mécanismes Réactionnels, CNRS-UMR 7651, Ecole Polytechnique, Palaiseau, France.
Renou-Gonnord
M F
MF
D000596:Amino Acids / Q000097:blood*; D002648:Child; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids