Homogeneous assays based on deoxyribozyme catalysis.
We report herein the first homogeneous assays based on the ribonuclease activity of a deoxyribozyme. The previously reported deoxyribozyme was covalently modified with biotin and used to assay biotin-binding interactions through changes in fluorescence upon substrate turnover. Deoxyribozymes with fluorescence-based reporting have the potential to serve as general analytical tools.
Stojanovic
M N
MN
Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, USA. mns18@columbia.edu
de Prada
P
P
Landry
D W
DW
D000494:Allosteric Regulation; D001483:Base Sequence; D001665:Binding Sites; D001710:Biotin / Q000378:metabolism / Q000494:pharmacology; D002384:Catalysis; D021881:DNA, Catalytic; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D004735:Energy Transfer; D005453:Fluorescence; D007700:Kinetics; D008024:Ligands; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000008:administration & dosage / Q000494:pharmacology; D013379:Substrate Specificity
D021881:DNA, Catalytic; D004277:DNA, Single-Stranded; D008024:Ligands; D009841:Oligonucleotides; D001710:Biotin; D019809:Streptavidin