[Development and approbation of polymerase chain reaction for detection of pathogen in Listeria infection].
A system of primers for detection of Listeria by polymerase chain reaction (PCR) has been developed. Specificity and sensitivity of the method was evaluated by analyses of reference clinical and abiotic samples. Clinical trials of PCR were carried by detecting latent (asymptomatic) carriers of Listeria among healthy women of epidemiologically significant professions. PCR is characterized by numerous advantages in comparison with the traditional bacteriological analysis.
Krasovskiĭ
V V
VV
Pokhil
S I
SI
Limanskiĭ
A P
AP
Limanskaia
O Iu
OIu
Timchenko
E N
EN
D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008087:Listeria / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000175:diagnosis* / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial