Sequence analysis or rat integrin alphaE1 and alphaE2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph.
The integrin alphaOX-62 subunit is defined by the OX-62 monoclonal antibody that was raised against rat dendritic cells in lymph (veiled cells) and shows properties similar to those of human alphaE that is predominantly expressed on intraepithelial lymphocytes. To clone alphaOX-62, rat probes generated using primers specific for the human alphaE sequence were used to screen rat T cell cDNA libraries. cDNA clones encoding two similar but not identical alpha subunits that are closely related but distinct from human alphaE were isolated. alphaE1 is predicted to be the rat homolog of mouse alphaM290 and alphaE2 corresponds to rat alphaOX-62. Immunofluorescence analysis revealed that mouse alphaE1 and rat alphaE2 are expressed in dendritic epidermal T cells in the skin, intraepithelial lymphocytes in the small intestine and in cells with a dendritic morphology present at sites where gammadelta T cells occur in lymphoid organs. Unexpectedly, in veiled cells alphaE2 is co-expressed with intracellular CD3-delta and a 33-kDa CD3 chain but not the T cell receptor. These findings suggest that veiled cells may be derived from a lymphoid precursor. Furthermore, veiled cells show phenotypic similarities to intraepithelial lymphocytes.
Brenan
M
M
Sir William Dunn School of Pathology, University of Oxford, Headinton. mbrenan@molbiol.ox.ac.uk
Rees
D J
DJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D017252:CD3 Complex / Q000096:biosynthesis / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D039001:Integrin alpha Chains; D007424:Intracellular Fluid / Q000378:metabolism; D008196:Lymph / Q000166:cytology; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D051381:Rats; D017421:Sequence Analysis; D014018:Tissue Distribution
D000596:Amino Acids; D015703:Antigens, CD; D017252:CD3 Complex; D018076:DNA, Complementary; D039001:Integrin alpha Chains; C091367:alpha E integrins