Serum-free coculture of stromal and epithelial cells from benign prostatic hyperplasia with keratinocyte growth factor.
We developed a serum-free coculture model of benign prostatic hyperplasia (BPH) to clarify whether stromal cells stimulate growth of epithelial cells from BPH tissues. Epithelial and stromal cells from freshly isolated BPH tissue were cultured separately in defined serum-free WAJC 404/RPMI 1640 medium supplemented with insulin, transferrin, selenium, hydrocortisone, bovine serum albumin, epidermal growth factor, basic fibroblast growth factor and keratinocyte growth factor. (3)H-Tdr incorporation into epithelial cells and stromal cells was used as a measure of proliferation. When epithelial cells were cocultured with stromal cells, (3)H-Tdr incorporation into epithelial cells was increased in comparison to that in epithelial cells cultured alone. Dihydrotestosterone significantly increased this effect. It is likely that the in vitro coculture model reported here will be useful for isolating and understanding stromal cell-derived paracrine growth factor(s).
Hashiba
T
T
Department of Urology, Yokohama City University School of Medicine, Yokohama, Japan.
Noguchi
S
S
Tsuchiya
F
F
Takeda
M
M
Masuda
M
M
Kubota
Y
Y
Hosaka
M
M
D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000473:pathology; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006133:Growth Substances; D006801:Humans; D015603:Keratinocytes; D008297:Male; D011470:Prostatic Hyperplasia / Q000473:pathology*; D017154:Stromal Cells / Q000473:pathology
D017077:Culture Media, Conditioned; C496453:FGF7 protein, human; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors