title: Evolution of circulating wild poliovirus and of vaccine-derived poliovirus in an immunodeficient patient: a unifying model.
abstract: We determined nucleotide sequences of the VP1 and 2AB genes and portions of the 2C and 3D genes of two evolving poliovirus lineages: circulating wild viruses of T geotype and Sabin vaccine-derived isolates from an immunodeficient patient. Different regions of the viral RNA were found to evolve nonsynchronously, and the rate of evolution of the 2AB region in the vaccine-derived population was not constant throughout its history. Synonymous replacements occurred not completely randomly, suggesting the need for conservation of certain rare codons (possibly to control translation elongation) and the existence of unidentified constraints in the viral RNA structure. Nevertheless the major contribution to the evolution of the two lineages came from linear accumulation of synonymous substitutions. Therefore, in agreement with current theories of viral evolution, we suggest that the majority of the mutations in both lineages were fixed as a result of successive sampling, from the heterogeneous populations, of random portions containing predominantly neutral and possibly adverse mutations. As a result of such a mode of evolution, the virus fitness may be maintained at a more or less constant level or may decrease unless more-fit variants are stochastically generated. The proposed unifying model of natural poliovirus evolution has important implications for the epidemiology of poliomyelitis.
authors.affiliation: A. N. Belozersky Institute of Physical-Chemical Biology, Moscow State University, Moscow 119899, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Gavrilin
authors.forename: E A
authors.initials: EA
authors.lastname: Cherkasova
authors.forename: G Y
authors.initials: GY
authors.lastname: Lipskaya
authors.forename: O M
authors.initials: OM
authors.lastname: Kew
authors.forename: V I
authors.initials: VI
authors.lastname: Agol
mesh_terms: D000293:Adolescent; D000595:Amino Acid Sequence; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002648:Child; D003062:Codon; D003546:Cysteine Endopeptidases / Q000235:genetics; D019143:Evolution, Molecular; D016679:Genome, Viral; D006801:Humans; D016867:Immunocompromised Host; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D011051:Poliomyelitis / Q000821:virology*; D017955:Poliovirus / Q000145:classification / Q000235:genetics*; D011055:Poliovirus Vaccine, Oral; D017422:Sequence Analysis, DNA; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000235:genetics; D014764:Viral Proteins
chemical_list: C101758:2B protein, poliovirus; D036022:Capsid Proteins; D003062:Codon; D011055:Poliovirus Vaccine, Oral; C442540:VP1 protein, Poliovirus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus