title: Micro-spoon technique in estimation of sperm concentration in rat epididymis.
abstract: Several technical procedures were described for estimation of epididymal sperm concentration until today. In all of the described techniques, epididymal material was drawn into a micro-pipette before counting procedure. We aimed to proceed this stage without using any pipette because of the difficulty in aspirating very viscous epididymal material into a micro-pipette. For measuring epididymal material we used a 2 microl reservoir which we named as a micro-spoon. It was filled with the epididymal material and layered in a tube with Ringer's solution of 2 ml containing sodium hyaluronate. Then, it was mixed in order to obtain 1/1000 diluted suspension. Sperm cells in this suspension were counted on a Makler chamber under a light microscope. The procedure was easy and time saving, compared to the other techniques.
authors.affiliation: Department of Urology, Yüzüncü Yil University School of Medicine, Van Turkey. sabahattinaydin@hotmail.com
authors.forename: O
authors.initials: O
authors.lastname: Odabaş
authors.forename: H
authors.initials: H
authors.lastname: Timurkan
authors.forename: M K
authors.initials: MK
authors.lastname: Atilla
authors.forename: Y
authors.initials: Y
authors.lastname: Yilmaz
authors.forename: E
authors.initials: E
authors.lastname: Sengül
authors.forename: S
authors.initials: S
authors.lastname: Aydin
mesh_terms: D000818:Animals; D004822:Epididymis / Q000166:cytology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D013997:Time Factors