title: Simultaneous determination of alpha-tocopherol and beta-carotene in olive oil by reversed-phase high-performance liquid chromatography.
abstract: A reversed-phase high-performance liquid chromatographic method was developed for the determination, in one run, of alpha-tocopherol and beta-carotene in virgin olive oil. The method involved a rapid saponification and a later extraction with a mixture of hexane-ethyl acetate. The chromatographic system consists of an ODS-2 column with a mobile phase of methanol-water-butanol and a diode-array detector. Linearity, precision, recovery and sensitivity were satisfactory. The main advantage of the proposed method is the speed and simultaneous determination of both compounds at the same time.
authors.affiliation: Dpt. Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gimeno
authors.forename: E
authors.initials: E
authors.lastname: Calero
authors.forename: A I
authors.initials: AI
authors.lastname: Castellote
authors.forename: R M
authors.initials: RM
authors.lastname: Lamuela-Raventós
authors.forename: M C
authors.initials: MC
authors.lastname: de la Torre
authors.forename: M C
authors.initials: MC
authors.lastname: López-Sabater
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000069463:Olive Oil; D010938:Plant Oils / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E / Q000032:analysis*; D019207:beta Carotene / Q000032:analysis*
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D019207:beta Carotene; D014810:Vitamin E