Mass spectrometry: a tool for the identification of proteins separated by gels.
Mass spectrometry (MS) has become the technique of choice to identify proteins. This has been largely accomplished by the combination of high-resolution two-dimensional (2-D) gel separation with robotic sample preparation, automated MS measurement, data analysis, and database query. Developments during the last five years in MS associated with protein gel separation are reviewed.
Lahm
H W
HW
F. Hoffmann-LaRoche Ltd., Pharmaceutical Research, Roche Genetics, Basel, Switzerland. hans-w.lahm@roche.com
Langen
H
H
D001331:Automation; D003628:Database Management Systems; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry / Q000379:methods*; D010449:Peptide Mapping; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification
D011506:Proteins