title: Cytoskeletal inhibitors suppress the stomatal opening of Vicia faba L. induced by fusicoccin and IAA.
abstract: Stomatal movement is governed by osmotic potential, in which K(+) concentration plays the dominant role. Our previous work has shown that both microtubules and microfilaments are involved in regulating stomatal movement. In the present investigation the relationships between cytoskeletal components and K(+) fluxes in stomatal opening were addressed by using fusicoccin (FC), indoleacetic acid (IAA), and cytoskeletal inhibitors to treat both epidermal strips and protoplasts of guard cells. The results revealed that FC and IAA induced stomatal opening with or without KCl in the dark. Also FC or IAA induced guard cell protoplast swelling in the dark even without added KCl. However, the induction was partially suppressed when strips and protoplasts were pretreated with cytochalasin B (CB), an inhibitor of F-actin polymerization, or oryzalin, an inhibitor of plant microtubule polymerization. Thus our preliminary results indicate for the first time that microtubules and microfilaments can affect stomatal opening independently of K(+) fluxes.
authors.affiliation: Biotechnology Research Center, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China
authors.initials: RF
authors.lastname: Huang
authors.initials: XC
authors.lastname: Wang
authors.initials: CH
authors.lastname: Lou