Human T-cell lymphotropic virus type-I tax gene induces interleukin-8 secretion by autocrine mechanism and has No effect on interleukin-16 in transfected Jurkat cells.
Human T-cell lymphotropic virus I (HTLV-I) Tax has been shown to transactivate several cellular genes. In this study, we show that interleukin-8 (IL-8) is expressed and secreted in tax-transfected Jurkat cells that were further augmented by mitogen stimulation. Expression of high-affinity IL-8-R (Type A) mRNA in these cells suggests an autocrine role for this chemokine in HTLV-I-infected T-cells. However, interleukin-16 (IL-16) mRNA expression or protein secretion was not significantly modulated either constitutively or even upon mitogen stimulation in these tax-transfected cells.
Zhang
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Laboratory of Cytokine Research, University of West Florida, Pensacola, Florida 32514, USA.
Sharma
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V
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D016356:Gene Products, tax / Q000235:genetics*; D006801:Humans; D019410:Interleukin-16 / Q000235:genetics / Q000378:metabolism*; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D019169:Jurkat Cells; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D017931:DNA Primers; D016356:Gene Products, tax; D019410:Interleukin-16; D016209:Interleukin-8; D012333:RNA, Messenger