title: Infection of white rat peritoneal macrophages with Toxoplasma gondii, (Coccidia: Sarcocystidae) after Trypanosoma lewisi (Kinetoplastida: Trypanosomatidae) infection.
abstract: Peritoneal macrophages from Wistar rats, inoculated and non-inoculated with 10(6) T. lewisi trypomastigotes, were cultured and infected with 10(6) T. gondii tachyzoites. Multiplication rates of this parasite were studied after 1, 24 and 48 h of infection but there were not significant differences between the number of parasites found inside of macrophages coming, either from T. lewisi infected or non infected rats. On the other hand, in vivo studies of Toxoplasma multiplication inside peritoneal macrophages, showed that there is an increase of parasite number in cells from T. lewisi infected rats, as compared with those macrophages from non infected rats. This effect was statistically significant and was more evident after four days of infection. Therefore, it has been demonstrated that in vivo, but not in vitro T. lewisi infections, causes an important decrease of the natural resistance to T. gondii of the white rats, which is manifested by the major invasion and multiplication of the parasite inside of peritoneal macrophages.
authors.affiliation: Instituto de Acueductos y Alcantarillados, San José Costa Rica. ciet@cariari.ucr.ac.cr
authors.forename: G
authors.initials: G
authors.lastname: Catarinella
authors.forename: M
authors.initials: M
authors.lastname: Chinchilla
authors.forename: O M
authors.initials: OM
authors.lastname: Guerrero
authors.forename: A
authors.initials: A
authors.lastname: Castro
mesh_terms: D000818:Animals; D007113:Immunity, Innate; D017737:Macrophages, Peritoneal / Q000469:parasitology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014122:Toxoplasma / Q000254:growth & development*; D014123:Toxoplasmosis / Q000276:immunology*; D014350:Trypanosoma lewisi / Q000276:immunology*