Kinetic studies on Ce(IV)-induced hydrolysis of single-stranded and double-stranded oligonucleotides.
The Ce(IV)-induced hydrolyses of DNA are kinetically investigated. The formation constants of the Ce(IV)-DNA complexes are in the following order: the single-stranded DNA > the double-stranded DNA >> the dinucleotide. On the other hand, the catalytic rate constants for the single-stranded DNA and the double-stranded DNA are comparable with each other, but both of them are much smaller than the value for the dinucleotide hydrolysis.
Igawa
T
T
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Sumaoka
J
J
Komiyama
M
M
D002563:Cerium / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D007700:Kinetics
D004277:DNA, Single-Stranded; D015226:Dinucleoside Phosphates; D002563:Cerium; D004247:DNA