The effect of growth temperature on the long-chain alkenes composition in the marine coccolithophorid Emiliania huxleyi.
The hydrocarbon fraction of a pure culture of Emiliania huxleyi, composed of a mixture of C31, C33, C37 and C38 polyunsaturated n-alkenes, appeared strongly dependent on the growth temperature of the alga between 8 degrees C and 25 degrees C. The total hydrocarbon content increased linearly with decreasing temperatures. C37 and C38 alkenes (which accounted for more than 90% of the total hydrocarbons) showed distinct changes in distribution compared to C31 and C33 alkenes, suggesting different biological syntheses and/or functions for these two groups of compounds. C37 and C38 alkenes and C37 methyl ketones (alkenones) all showed a trend to lower proportions of the two diunsaturated isomers and to higher proportions of the corresponding trienes with decreasing temperature. Unlike the alkenone unsaturation ratio (U37k'), ratios based on the C37 and C38 alkadi- and trienes could be linearly related to the growth temperature of E. huxleyi only between 15 degrees C and 25 degrees C. The modifications in the distribution of alkenes induced by varying temperature appeared, however, to be twice as fast as the modifications undergone by the alkenones. Although structurally and biochemically related, the distinct evolutions of alkenes and alkenones in response to changes in growth temperature might indicate that these two classes of compounds play two distinct physiological functions. The non-systematic linearity of relationships to temperature of parameters based on alkenes distribution suggested that these compounds are of limited use as paleotemperature indicator in the marine environment in contrast with the alkenones.
Grossi
V
V
Laboratoire d'Océanographie et de Biogéochimie (UMR CNRS 6535), Centre d'Océanologie de Marseille (OSU), France. grossi@com.univ-mrs.fr
Raphel
D
D
Aubert
C
C
Rontani
J F
JF
D000475:Alkenes / Q000032:analysis*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D056890:Eukaryota / Q000737:chemistry* / Q000254:growth & development; D009682:Magnetic Resonance Spectroscopy; D008386:Marine Biology; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D000475:Alkenes