title: Antigenic and sequence diversity in gonococcal transferrin-binding protein A.
abstract: Neisseria gonorrhoeae is a gram-negative pathogen that is capable of satisfying its iron requirement with human iron-binding proteins such as transferrin and lactoferrin. Transferrin-iron utilization involves specific binding of human transferrin at the cell surface to what is believed to be a complex of two iron-regulated, transferrin-binding proteins, TbpA and TbpB. The genes encoding these proteins have been cloned and sequenced from a number of pathogenic, gram-negative bacteria. In the current study, we sequenced four additional tbpA genes from other N. gonorrhoeae strains to begin to assess the sequence diversity among gonococci. We compared these sequences to those from other pathogenic bacteria to identify conserved regions that might be important for the structure and function of these receptors. We generated polyclonal mouse sera against synthetic peptides deduced from the TbpA sequence from gonococcal strain FA19. Most of these synthetic peptides were predicted to correspond to surface-exposed regions of TbpA. We found that, while most reacted with denatured TbpA in Western blots, only one antipeptide serum reacted with native TbpA in the context of intact gonococci, consistent with surface exposure of the peptide to which this serum was raised. In addition, we evaluated a panel of gonococcal strains for antigenic diversity using these antipeptide sera.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, Virginia 23298, USA. cncornel@hsc.vcu.edu
authors.forename: C N
authors.initials: CN
authors.lastname: Cornelissen
authors.forename: J E
authors.initials: JE
authors.lastname: Anderson
authors.forename: I C
authors.initials: IC
authors.lastname: Boulton
authors.forename: P F
authors.initials: PF
authors.lastname: Sparling
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D000940:Antigenic Variation / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology; D003429:Cross Reactions; D033862:Iron-Binding Proteins; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000235:genetics* / Q000276:immunology; D011990:Receptors, Transferrin / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D033863:Transferrin-Binding Proteins
chemical_list: D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D011990:Receptors, Transferrin; D033863:Transferrin-Binding Proteins