[Construction and expression of anticolonic cancer scFv fragment].
Anticolonic cancer scFv fragment with a VH-linker-VL structure was constructed and expressed in E. coli. SDS-PAGE analysis showed the fragment cloned in pComb3 was not expressed efficiently in JM83, while cloned in pET-22b(+) highly expressed in BL21(DE3) up to 35.5% of the total bacterial protein obtained, if the culture was incubated under 30 degrees C.
Song
L X
LX
Institute of Biotechnology, Academy of Military Medical Science, Beijing.
Yu
W Y
WY
D000595:Amino Acid Sequence; D000912:Antibodies, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D003110:Colonic Neoplasms / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis*
D000912:Antibodies, Neoplasm; D007128:Immunoglobulin Fragments; D011994:Recombinant Proteins; C055966:immunoglobulin Fv