Substrate specificity of regiospecific desaturation of aliphatic compounds by a mutant Rhodococcus strain.
Substrate specificity of cis-desaturation of alipahtic compounds by resting cells of a mutant, Rhodococcus sp. strain KSM-MT66, was examined. Among substrates tested, the rhodococcal cells were able to convert n-alkanes (C13-C19), 1-chloroalkanes (C16 and C18), ethyl fatty acids (C14-C17) and alkyl (C1-C4) esters of palmitic acid to their corresponding unsaturated products of cis configuration. The products from n-alkanes and 1-chloroalkanes had a double bond mainly at the 9th carbon from their terminal methyl groups, and the products from acyl fatty acids had a double bond mainly at the 6th carbon from their carbonyl carbons.
Koike
K
K
Tochigi Research Laboratories of Kao Corporation, Ichikai, Haga, Japan. 381128@kastanet.kao.co.jp
Takaiwa
M
M
Kimura
Y
Y
Inoue
S
S
Ito
S
S
D005227:Fatty Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006838:Hydrocarbons / Q000378:metabolism*; D015394:Molecular Structure; D009154:Mutation; D012240:Rhodococcus / Q000378:metabolism*; D013379:Substrate Specificity
D005227:Fatty Acids; D006838:Hydrocarbons