Comparative study of seeding methods for three-dimensional polymeric scaffolds.
Development of tissue-engineered devices may be enhanced by combining cells with porous absorbable polymeric scaffolds before implantation. The cells are seeded throughout the scaffolds and allowed to proliferate in vitro for a predetermined amount of time. The distribution of cells throughout the porous material is one critical component determining success or failure of the tissue-engineered device. This can influence both the successful integration of the device with the host tissue as well as the development of a vascularized network throughout the entire scaffold volume. This research sought to compare different seeding and proliferation methods to select an ideal method for a polyglycolide/aortic endothelial cell system. Two seeding environments, static and dynamic, and three proliferation environments, static, dynamic, and bioreactor, were analyzed, for a total of six possible methods. The six seeding and proliferation combinations were analyzed following a 1-week total culture time. It was determined that for this specific system, dynamic seeding followed by a dynamic proliferation phase is the least promising method and dynamic seeding followed by a bioreactor proliferation phase is the most promising.
Burg
K J
KJ
Department of Bioengineering, Clemson University, Clemson, South Carolina 29634-0905, USA.
Holder
W D
WD
Culberson
C R
CR
Beiler
R J
RJ
Greene
K G
KG
Loebsack
A B
AB
Roland
W D
WD
Eiselt
P
P
Mooney
D J
DJ
Halberstadt
C R
CR
D000818:Animals; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D019149:Bioreactors; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid; D051381:Rats
D001672:Biocompatible Materials; D011100:Polyglycolic Acid