title: Analysis of human rotavirus G serotype in Bangladesh by enzyme-linked immunosorbent assay and polymerase chain reaction.
abstract: Distribution of human rotavirus G serotype was investigated by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) with faecal specimens obtained from children with diarrhoea in Bangladesh. By ELISA, subgroup and G serotype were determined for 59.5% and 28.6% of group A rotavirus-positive specimens respectively. However, of the 120 specimens, the G serotype of which was not determined by ELISA, serotype of the 112 specimens was typed by PCR. In total, G serotype was assigned for 95.2% of all the specimens, showing the highest rate of G4 (41.7%), followed by G1 (23.2%) and G2 (14.9%). Twenty-four specimens showed mixed types, such as G2 with G1, G8 or G9, or G1 with G4. These results indicate that PCR combined with ELISA is highly effective for G serotyping of rotavirus.
authors.affiliation: Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
authors.forename: M U
authors.initials: MU
authors.lastname: Ahmed
authors.forename: M M
authors.initials: MM
authors.lastname: Alam
authors.forename: N S
authors.initials: NS
authors.lastname: Chowdhury
authors.forename: M M
authors.initials: MM
authors.lastname: Haque
authors.forename: N
authors.initials: N
authors.lastname: Shahid
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: T
authors.initials: T
authors.lastname: Urasawa
authors.forename: S
authors.initials: S
authors.lastname: Urasawa
mesh_terms: D001459:Bangladesh; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification*; D012400:Rotavirus Infections / Q000821:virology*; D012703:Serotyping