title: Structure of an acidic O-specific polysaccharide of the bacterium Providencia alcalifaciens O7.
abstract: An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O7 and purified by gel chromatography followed by anion-exchange chromatography. On the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1H- and 13C-NMR spectroscopy, including two-dimensional 1H,1H homonuclear and H-detected 1H,13C heteronuclear correlation spectroscopy and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: [figure], where Rhap2Ac is 2-O-acetylrhamnopyranose.
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia. knirel@ioc.ac.ru
authors.forename: O V
authors.initials: OV
authors.lastname: Bystrova
authors.forename: G V
authors.initials: GV
authors.lastname: Zatonskii
authors.forename: S A
authors.initials: SA
authors.lastname: Borisova
authors.forename: N A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: E V
authors.initials: EV
authors.lastname: Kholodkova
authors.forename: E S
authors.initials: ES
authors.lastname: Stanislavskii
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry*
chemical_list: D019081:O Antigens