title: Diagnosis of rinderpest in Tanzania by a rapid chromatographic strip-test.
abstract: A simple chromatographic strip-test based on Clearview technology, is under development as a pen-side test for the detection of rinderpest antigen in eye swabs taken from cattle in the field. An outbreak of rinderpest occurred in the northern zone of Tanzania from late February to June 1997. The affected cattle exhibited very mild clinical signs, which made clinical diagnosis difficult. One hundred and seven eye swabs were collected from cattle suspected of infection with rinderpest. These were tested in the field using a prototype of the pen-side test and 13 (12.15%) of the samples were found to be positive for the presence of rinderpest antigen. These were confirmed by ICE. The positive cases were predominantly found in the Ngorongoro district. This demonstrates the usefulness of such a simple, rapid pen-side diagnostic assay, particularly when clinically 'mild' strains of rinderpest are present.
authors.affiliation: Animal Diseases Research Institute, Dar Es Salaam, Tanzania. p.wambura@mailbox.uq.edu.au
authors.forename: P N
authors.initials: PN
authors.lastname: Wambura
authors.forename: D W
authors.initials: DW
authors.lastname: Moshy
authors.forename: A N
authors.initials: AN
authors.lastname: Mbise
authors.forename: G O
authors.initials: GO
authors.lastname: Mollel
authors.forename: W P
authors.initials: WP
authors.lastname: Taylor
authors.forename: J
authors.initials: J
authors.lastname: Anderson
authors.forename: A
authors.initials: A
authors.lastname: Bruning
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D002845:Chromatography / Q000662:veterinary; D004196:Disease Outbreaks / Q000662:veterinary; D008863:Microspheres; D018103:Morbillivirus / Q000302:isolation & purification*; D012301:Rinderpest / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D013636:Tanzania / Q000453:epidemiology; D013666:Tears / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral