Efficacy of nitazoxanide, tizoxanide and tizoxanide glucuronide against Cryptosporidium parvum development in sporozoite-infected HCT-8 enterocytic cells.
The effects of nitazoxanide and its metabolites, tizoxanide and tizoxanide glucuronide, on the development of the asexual and sexual stages of Cryptosporidium parvum in differentiated human enterocytic HCT-8 cells were evaluated in a quantitative alkaline phosphatase immunoassay. Nitazoxanide, tizoxanide and tizoxanide glucuronide were inhibitory for up to 46 h when added after sporozoite invasion (MIC50 1.2, 22.6 and 2.2 mg/L, respectively). Tizoxanide had only limited activity, but nitazoxanide and tizoxanide glucuronide strongly inhibited asexual and sexual stages, respectively.
Gargala
G
G
Laboratoire d'Immunologie et Immunopathologie, UPRES-EA 2128, CHU, 14033 Caen, France.
Delaunay
A
A
Li
X
X
Brasseur
P
P
Favennec
L
L
Ballet
J J
JJ
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002460:Cell Line; D016785:Cryptosporidium parvum / Q000187:drug effects*; D020895:Enterocytes / Q000469:parasitology; D020719:Glucuronides / Q000494:pharmacology*; D006801:Humans; D009574:Nitro Compounds; D013844:Thiazoles / Q000494:pharmacology*
D000981:Antiprotozoal Agents; D020719:Glucuronides; D009574:Nitro Compounds; D013844:Thiazoles; C104067:tizoxanide; C041747:nitazoxanide