Fluorescent probes: looking backward and looking forward.
Probe technology has been advancing very rapidly but to study molecular events in real time, there has to be a discrete choice in the use of "probes" and the "labels" that they carry. In this minireview, we shed light on the use of fluorescent probes, especially the use of green fluorescent protein (GFP) and its variants as tools to cell biologists studying protein secretion and trafficking. The use of these GFP variants has further widened the application of Fluorescence Resonance Energy Transfer (FRET) in analyzing protein-protein interaction.
Latif
R
R
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10128, USA. rauf.latif@mssm.edu
Graves
P
P
D004704:Endocrinology / Q000379:methods*; D004735:Energy Transfer; D005453:Fluorescence; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006801:Humans; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D011506:Proteins / Q000378:metabolism; D013961:Thyroid Gland / Q000378:metabolism*
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D011506:Proteins; D049452:Green Fluorescent Proteins