Observations on the covalent cross-linking of the binding domain (BD) of the high mobility group I/Y (HMG I/Y) proteins to DNA by FR66979.
FR66979, a drug closely related to the mitomycin C class of antitumor antibiotics, is shown to covalently cross-link DNA to the DNA-binding domain of the High Mobility Group I/Y (HMG I/Y) DNA-binding proteins in the minor groove.
Rajski
S R
SR
Department of Chemistry, Colorado State University, Fort Collins 80523, USA.
Williams
R M
RM
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010078:Oxazines / Q000737:chemistry; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D003432:Cross-Linking Reagents; D006609:High Mobility Group Proteins; D010078:Oxazines; D014157:Transcription Factors; C058745:FR 66979; D025741:HMGA1a Protein; D004247:DNA