title: Effect of renal denervation on renin gene expression, concentration, and secretion in mature ovine fetus.
abstract: To determine the role of the renal nerves on renin secretion and expression in the mature ovine fetus, we performed bilateral renal denervation on eight fetuses of time-dated pregnant ewes (126.8 +/- 0.6 days gestation) and compared renin in them to seven fetuses that underwent sham denervation (126.7 +/- 0.6 days gestation). Fetal arterial and venous catheters were implanted, and after 5-7 days of recovery isoproterenol was infused. Plasma active renin was lower in denervated animals than in intact animals under basal conditions and at each dose of isoproterenol. Plasma prorenin levels were lower in denervated fetuses but unaffected by isoproterenol. Denervation did not change renal renin, prorenin, or renin mRNA, but it did block isoproterenol-induced increases in renin mRNA in renocortical cells in vitro. We conclude that the renal nerves are required for renin secretory mechanisms and responsiveness of renin mRNA to beta-adrenergic stimulation but not for the expression of renin in the fetal kidney. We propose that one or more of the factors that maintain renin expression in the perinatal period may be absent or may be replaced by the renal nerves in the adult.
authors.affiliation: Department of Obstetrics and Gynecology, and the Perinatal Research Laboratories, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. michaeldraper@ouhsc.edu
authors.forename: M L
authors.initials: ML
authors.lastname: Draper
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Valego
authors.forename: W A
authors.initials: WA
authors.lastname: Block
authors.forename: J C
authors.initials: JC
authors.lastname: Rose
mesh_terms: D000818:Animals; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000187:drug effects; D002478:Cells, Cultured; D003714:Denervation; D004305:Dose-Response Relationship, Drug; D004573:Electrolytes / Q000097:blood; D004792:Enzyme Precursors / Q000097:blood; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D006340:Heart Rate, Fetal / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007262:Infusions, Intravenous; D007545:Isoproterenol / Q000008:administration & dosage; D007668:Kidney / Q000737:chemistry / Q000196:embryology* / Q000294:innervation* / Q000378:metabolism; D007672:Kidney Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009638:Norepinephrine / Q000032:analysis; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D012083:Renin / Q000097:blood* / Q000235:genetics*; D012756:Sheep
chemical_list: D004573:Electrolytes; D004792:Enzyme Precursors; D012333:RNA, Messenger; D012083:Renin; D007545:Isoproterenol; D009638:Norepinephrine