A semi-quantitative PCR method for the detection of low levels of apoptotic DNA fragmentation in a heart failure model.
Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.
McLachlan
C S
CS
Department of Pathology, University of Sydney, Camperdown, NSW, Australia. pmac@zip.com.au
Yin
J L
JL
Driussi
C
C
Jusuf
P R
PR
Hambly
B
B
McGuire
M A
MA
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D006333:Heart Failure / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits