title: The structure of the transcriptional antiterminator NusB from Escherichia coli.
abstract: We have determined the solution structure of NusB, a transcription antitermination protein from Escherichia coli. The structure reveals a novel, all alpha-helical protein fold. NusB mutations that cause a loss of function (NusB5) or alter specificity for RNA targets (NusB101) are localized to surface residues and likely affect RNA-protein or protein-protein interactions. Residues that are highly conserved among homologs stabilize the protein core. The solution structure of E. coli NusB presented here resembles that of Mycobacterium tuberculosis NusB determined by X-ray diffraction, but differs substantially from a solution structure of E. coli NusB reported earlier.
authors.affiliation: Structural Biophysics Laboratory, National Cancer Institute-FCRDC, Frederick, MD 21702, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Altieri
authors.forename: M J
authors.initials: MJ
authors.lastname: Mazzulla
authors.forename: D A
authors.initials: DA
authors.lastname: Horita
authors.forename: R H
authors.initials: RH
authors.lastname: Coats
authors.forename: P T
authors.initials: PT
authors.lastname: Wingfield
authors.forename: A
authors.initials: A
authors.lastname: Das
authors.forename: D L
authors.initials: DL
authors.lastname: Court
authors.forename: R A
authors.initials: RA
authors.lastname: Byrd
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D029968:Escherichia coli Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012313:RNA / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012996:Solutions; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C074952:NusB protein, E coli; D016601:RNA-Binding Proteins; D012996:Solutions; D014157:Transcription Factors; D012313:RNA