title: Identification of an EDG7 variant, HOFNH30, a G-protein-coupled receptor for lysophosphatidic acid.
abstract: We have identified a cDNA, designated HOFNH30, which encodes a 354 amino acid G-protein-coupled receptor (GPCR). This receptor has 96% amino acid identity to the Jurkat-T cell-derived EDG7 and could be a splice variant. RT-PCR analysis demonstrated that HOFNH30 mRNA is expressed in placenta whereas EDG7 mRNA shows highest expression in prostate. The HOFNH30 gene is localized to human chromosome 1p22. 3-1p31.1. When HOFNH30 was expressed in RBL-2H3 cells, LPA and phosphatidic acid (PA) induced a calcium mobilization response with EC(50) values of 13 nM and 3 microM, respectively. LPA also induced phosphorylation of mitogen-activated protein kinase (p42(MAPK) and p44(MAPK)) in HOFNH30-transfected but not vector-transfected RBL-2H3 cells. In the present study, we have identified a novel variant from the EDG receptor family, a GPCR for which LPA is a high-affinity endogenous ligand.
authors.affiliation: Department of Renal Biology, Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, King of Prussia, Pennsylvania 19406, USA.
authors.forename: L R
authors.initials: LR
authors.lastname: Fitzgerald
authors.forename: G M
authors.initials: GM
authors.lastname: Dytko
authors.forename: H M
authors.initials: HM
authors.lastname: Sarau
authors.forename: I J
authors.initials: IJ
authors.lastname: Mannan
authors.forename: C
authors.initials: C
authors.lastname: Ellis
authors.forename: P A
authors.initials: PA
authors.lastname: Lane
authors.forename: K B
authors.initials: KB
authors.lastname: Tan
authors.forename: P R
authors.initials: PR
authors.lastname: Murdock
authors.forename: S
authors.initials: S
authors.lastname: Wilson
authors.forename: D J
authors.initials: DJ
authors.lastname: Bergsma
authors.forename: R S
authors.initials: RS
authors.lastname: Ames
authors.forename: J J
authors.initials: JJ
authors.lastname: Foley
authors.forename: D A
authors.initials: DA
authors.lastname: Campbell
authors.forename: L
authors.initials: L
authors.lastname: McMillan
authors.forename: N
authors.initials: N
authors.lastname: Evans
authors.forename: N A
authors.initials: NA
authors.lastname: Elshourbagy
authors.forename: H
authors.initials: H
authors.lastname: Minehart
authors.forename: P
authors.initials: P
authors.lastname: Tsui
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D004789:Enzyme Activation; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008246:Lysophospholipids / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D008246:Lysophospholipids; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D019204:GTP-Binding Proteins; D002118:Calcium