[The activation of phagocytosis by recombinant human tumor necrosis factor-beta].
The functional activity of phagocytic cells of various types was studied in white non-inbred mice by administering recombinant human tumor necrosis beta (rhTNF-beta). It was shown that rhTNF-beta increased phagocytic activity of the peritoneal exudate, spleen and liver macrophages as well as blood polynuclears. Stimulation of neutrophils was demonstrated in earlier times after administration of the preparation as compared to macrophages (3 h and 24 h, respectively). The duration of the macrophage activation effect and its expression depended on the dose of the preparation and were the most notable when rhTNF-beta was administered in doses of 10(3)-10(5) U/20 g. The addition of reopolyglucin, the polysaccharide filler, didn't remove a stimulatory effect of rhTNF-beta on macrophages, but influenced its dynamics. Multiple administration of the preparation didn't cause the phagocytosis stimulation effect.
Masycheva
V I
VI
Research Design and Technology Institute of Biologically Active Substances, SRC VB "Vector", Berdsk, Novosibirsk Region.
Fadina
V A
VA
Danilenko
E D
ED
D000818:Animals; D002478:Cells, Cultured; D004912:Erythrocytes; D006801:Humans; D008233:Lymphotoxin-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D051379:Mice; D018375:Neutrophil Activation / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012756:Sheep; D013997:Time Factors
D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins