[A study on screening and high density cell cultivation of a yeast strain Kluyveromyces with high inulinase yielding and its enzymology properties].
A yeast strain Kluyveromyces with high inulinase yield was screened. The highest inulinase activity of 288.78 u/mL was reached when a high cell density cultivation method was developed for inulinase production. It was 6.8 times higher than the highest level reported in the same species. The activity ratio of its inulinase to invertase was 1/24.72, the Km values were 13.3 mmol/L and 62.6 mmol/L when inulin and sucrose were used as substrate, respectively; The optimum pH value was 4.4, this enzyme also showed a good pH adaptability and stability, i.e. more 90% of the highest level was maintained between pH 3.8 and 5.6; The optimum reaction temperature was 55 degrees C, higher activity was maintained between 50-57.5 degrees C, its half life period was 16 hours at 55 degrees C; It was found for the first time that addition of magnesium ion into the reaction system increased the enzyme activity by 11%.
Wang
J H
JH
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing.
Liu
Y Y
YY
Yao
B
B
Wang
Y R
YR
D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D007339:Insulysin / Q000378:metabolism*; D007716:Kluyveromyces / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
D007339:Insulysin; D008274:Magnesium