title: Dynamics of the development of Chlamydophila psittaci inclusions in epithelial and fibroblast host cells.
abstract: The development of Chlamydophila psittaci (formerly Chlamydia psittaci, avian strains) inclusions in fibroblast L-929 and epithelial BGM cell lines was studied along the bacterial growth cycle using a BGM cell-adapted strain in the presence or absence of cycloheximide and cycloheximide + polyethylene glycol. Evolution of the inclusions was determined in terms of their number and size at 24, 30, 36, 48 and 54 h after infection. Significant differences in the chlamydial growth were found between both host cells, throughout the study. Higher numbers of inclusions (P < 0.05) were observed in L cells while larger inclusions (P < 0.01) were found in BGM cells. In both fibroblast and epithelial cells, inclusions showed a significant (P < 0.001) increase in size at the later times studied. Free extracellular chlamydial particles were noticed at 48 and 54 h post-infection (p.i.) in infected L cells, and at 54 h p.i. in BGM cells. Addition of cycloheximide or cycloheximide + polyethylene glycol had no significant effect on the number of inclusions or their size. The results suggest that host cell characteristics and innate compatibility between Chlamydophila strain and host cell are more important than host cell adaptation for the development of the microorganism.
authors.affiliation: Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Escalante-Ochoa
authors.forename: R
authors.initials: R
authors.lastname: Ducatelle
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
mesh_terms: D000818:Animals; D002460:Cell Line; D002691:Chlamydophila psittaci / Q000254:growth & development*; D002522:Chlorocebus aethiops; D003470:Culture Media; D004847:Epithelial Cells / Q000382:microbiology*; D005347:Fibroblasts / Q000382:microbiology*; D051379:Mice
chemical_list: D003470:Culture Media