title: Embryonic cord transplants in peripheral nerve restore skeletal muscle function.
abstract: The rapid atrophy of skeletal muscle after denervation severely compromises efforts to restore muscle function. We have transplanted embryonic day 14-15 (E14-E15) ventral spinal cord cells into adult Fischer rat tibial nerve stump to provide neurons for reinnervation. Our aim was to evaluate medial gastrocnemius reinnervation physiologically because this transplant strategy will only be effective if the reinnervated muscle contracts, generates sufficient force to induce joint movement, and is fatigue resistant enough to shorten repeatedly. Twelve weeks posttransplantation, brief duration electrical stimuli applied to the transplants induced medial gastrocnemius contractions that were strong enough to produce ankle movement in 4 of 12 rats (33%). The force of these four "low-threshold" reinnervated muscles and control muscles declined only gradually during five hours of intermittent, supramaximal stimulation and without depression of EMG potential area, which is strong evidence of functional neuromuscular junctions and fatigue resistant muscles. Sectioning of the medial gastrocnemius nerves confirmed that these contractions were innervation dependent. Weakness in low-threshold reinnervated muscles (8% control force) related to incomplete reinnervation, reductions in muscle fiber size, specific tension, and/or the presence of nonfunctional neuromuscular junctions. Muscle reinnervation achieved using this novel transplantation strategy may salvage completely denervated muscle and may provide the potential to evoke limb movement when injury or disease precludes or delays peripheral axon regeneration.
authors.affiliation: The Miami Project to Cure Paralysis and Department of Neurological Surgery, University of Miami School of Medicine, Miami 33136, Florida, USA. cthomas@miamiproject.med.miami.edu
authors.forename: C K
authors.initials: CK
authors.lastname: Thomas
authors.forename: D E
authors.initials: DE
authors.lastname: Erb
authors.forename: R M
authors.initials: RM
authors.lastname: Grumbles
authors.forename: R P
authors.initials: RP
authors.lastname: Bunge
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D005260:Female; D016332:Fetal Tissue Transplantation; D009068:Movement / Q000502:physiology; D009121:Muscle Denervation; D018763:Muscle Fatigue / Q000502:physiology; D018908:Muscle Weakness / Q000503:physiopathology; D018482:Muscle, Skeletal / Q000294:innervation* / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D020127:Recovery of Function / Q000502:physiology; D013116:Spinal Cord / Q000637:transplantation*; D013979:Tibial Nerve / Q000502:physiology / Q000601:surgery*