title: Influence of dinitroglycerol and nitroethyleneglycol lipid derivatives on spectral parameters of human hemoglobin.
abstract: The interaction of organic nitrates (nitroethyleneglycol, dinitroglycerol, and their esters with arachidonic acid) with oxyhemoglobin and methemoglobin has been studied. Addition of nitroethyleneglycol and dinitroglycerol to oxyhemoglobin is accompanied by a modest but significant increase in oxidation rate of the heme protein to the high-spin ferri-form--methemoglobin. Arachidonoylglycerol dinitrate exerts a similar but more pronounced effect on hemoglobin: a molar excess of this dinitrate induces the transformation of a significant portion of oxyhemoglobin to methemoglobin, whereas arachidonoylnitroethyleneglycol is inactive. Arachidonoylglycerol dinitrate also induces changes in the spectral characteristics of methemoglobin; this may be due to disintegration of the methemoglobin with the loss of heme. The data demonstrate that some organic nitrates can interact with hemoglobin; this should be taken into account when using the oxyhemoglobin technique for measuring nitric oxide generation from these compounds.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117871, Russia. vvbez@oxylipin.ibch.ru
authors.forename: V V
authors.initials: VV
authors.lastname: Bezuglov
authors.forename: G M
authors.initials: GM
authors.lastname: Andreyuk
authors.forename: I V
authors.initials: IV
authors.lastname: Serkov
authors.forename: M A
authors.initials: MA
authors.lastname: Kisel
mesh_terms: D005026:Ethylene Glycols / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D005996:Nitroglycerin / Q000031:analogs & derivatives* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005026:Ethylene Glycols; D006454:Hemoglobins; C014509:dinitroglycerol; D005996:Nitroglycerin