title: Smooth muscle cell migration induced by shear-loaded platelets and endothelial cells. Enhanced platelet-derived growth factor production by shear-loaded platelets.
abstract: BACKGROUND
  Uncontrolled migration and proliferation of smooth muscle cells (SMC) are the main mechanisms of development of atherosclerotic neointima. However, it has not yet been elucidated how mechanical stress is involved in this process. We investigated smooth muscle cell mitogenic activity induced by shear-loaded platelets and endothelial cells.
  
  
  METHODS
  
  
  
  METHODS
  in vitro experimental study. We devised four types of conditioned media; supernatant of mixed culture of platelets and endothelial cell (ST), supernatant of shear-loaded mixed culture of platelets and endothelial cell (SH), ST medium neutralised with anti-PDGF antibody (ST+), and SH medium neutralised with anti-PDGF antibody (SH+). Smooth muscle cells were cultured in each conditioned medium, and their spreading activity was determined under a microscope.
  
  
  RESULTS
  Smooth muscle cells spreading activity in the SH group was significantly greater than that in the ST group. Their spreading activity was suppressed by anti-PDGF antibody under shear conditions (SH+), but it was not by anti-PDGF antibody under static conditions (ST+).
  
  
  CONCLUSIONS
  Our results demonstrate that platelet-derived growth factor is produced by shear-loaded platelets and endothelial cells, and local mechanical forces may play an important role in cardiovascular disease.
authors.affiliation: Department of Surgery, The University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakazawa
authors.forename: H
authors.initials: H
authors.lastname: Yasuhara
authors.forename: K
authors.initials: K
authors.lastname: Shigematsu
authors.forename: H
authors.initials: H
authors.lastname: Shigematsu
mesh_terms: D001792:Blood Platelets / Q000502:physiology*; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D018056:Hemorheology; D006801:Humans; D006965:Hyperplasia / Q000209:etiology; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000378:metabolism*; D011673:Pulsatile Flow; D013314:Stress, Mechanical; D017539:Tunica Intima / Q000473:pathology
chemical_list: D010982:Platelet-Derived Growth Factor