Separation of closely related peptide substrates of human proteinases by micellar electrokinetic chromatography with anionic and nonionic surfactants.
In order to use micellar electrokinetic chromatography to determine the proteolytic activity of different proteinases simultaneously present in physiological fluids, the technique must be able to separate mixtures of substrates with closely related structures. In an attempt to determine the best electrophoretic conditions for resolving six p-nitroanilide peptides used as synthetic substrates of the elastolytic enzymes (human neutrophil elastase, cathepsin G, Pseudomonas aeruginosa elastase) most commonly involved in pulmonary diseases, we investigated the efficiency of ionic and nonionic surfactants in achieving the separation of this complex mixture. The results presented here show that, of all the electrophoretic systems tested, 30 mM sodium tetraborate, pH 9.3, containing 25 mM Brij 35 as micellar agent offered the best performance; the separation efficiency of peptides is greater than that obtained with other reagents and all peaks are baseline resolved and unambiguously identifiable. Analysis of the micelle-solute interaction with the surfactants investigated allowed better definition of the mechanism involved in the distribution of these peptides to the micelles and identification of some structural features that determined the magnitude of the micelle peptide complex formation.
Lupi
A
A
Laboratorio di Biochimica e Genetica Clinica di Malattie dellApparato Respiratorio, IRCCS Policlinico San Matteo, Pavia, Italy.
Viglio
S
S
Luisetti
M
M
Zanaboni
G
G
Cetta
G
G
Iadarola
P
P
D056649:Cathepsin G; D002403:Cathepsins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D010450:Endopeptidases / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D019272:Leukocyte Elastase / Q000378:metabolism; D008823:Micelles; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D010196:Pancreatic Elastase / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D012697:Serine Endopeptidases; D013379:Substrate Specificity; D013501:Surface-Active Agents
D007202:Indicators and Reagents; D008823:Micelles; D009842:Oligopeptides; D013501:Surface-Active Agents; D002403:Cathepsins; D010450:Endopeptidases; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase