Cryopreservation of mouse spermatozoa.
Recently, it has become possible to freeze a large number of mouse spermatozoa immediately after collection from the epididymides of a small number of males. The cryopreservation of spermatozoa is simpler, less time-consuming, and less costly than that of embryos for maintaining various strains of mice with induced mutations. This chapter attempts to provide a realistic overview of the cryopreservation of mouse spermatozoa and to describe a detailed procedure for mouse sperm freezing.
Nakagata
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Division of Reproductive Engineering, Center for Animal Resources & Development, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan. nakagata@kaiju.medic.kumamoto-u.ac.jp
D000818:Animals; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods; D004624:Embryo Transfer; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice / Q000235:genetics*; D008815:Mice, Inbred Strains; D013094:Spermatozoa / Q000166:cytology*