DNA topology: feeling the pulse of a topoisomerase.
The action of individual type II DNA topoisomerases has been followed in real time by observing the elastic response of single DNA molecules to sequential strand passage events. Micromanipulation methods provide a complementary approach to biochemical studies for investigating the mechanism of DNA topoisomerases.
Stasiak
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Laboratoire d'Analyse Ultrastructurale, Bätiment de Biologie, Universté de Lausanne, Lausanne-Dorigny, CH-1015, Switzerland. Andrzej.Stasiak@lau.unil.ch.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry / Q000378:metabolism*; D066298:In Vitro Techniques; D008954:Models, Biological; D009690:Nucleic Acid Conformation
D004278:DNA, Superhelical; D000255:Adenosine Triphosphate; D004250:DNA Topoisomerases, Type II