DNA ploidy of human granulosa cells from natural and stimulated in vitro fertilization cycles.
OBJECTIVE
To analyze and compare the DNA ploidy of granulosa cells from natural and gonadotropin-stimulated follicles obtained during IVF.


METHODS
Retrospective analysis of laboratory data.


METHODS
University medical center.


METHODS
Seventy-three aspirates of dominant follicles from natural IVF cycles and 113 aspirates from gonadotropin-stimulated cycles were analyzed.


METHODS
Cytospins were prepared and stained by the Feulgen-thionine method.


METHODS
Image DNA analysis was performed on an automated high-resolution image cytometer. DNA content and the number of nuclei with DNA content >5c were measured.


RESULTS
All samples from natural and gonadotropin-stimulated follicles were found to be diploid. Single cells with DNA content >5c were found in follicular fluid samples of four women with natural IVF cycles and in samples of nine women with gonadotropin-stimulated cycles.


CONCLUSIONS
DNA ploidy of granulosa cells from natural follicles has not been studied before. In natural samples, granulosa cells were only diploid, without euploid polyploidization. We were unable to confirm DNA aneuploidy of granulosa cells in gonadotropin-stimulated follicles of women undergoing IVF.
Gersak
K
K
University Medical Center and Institute of Oncology, Ljubljana, Slovenia. ksenija.gersak@s-net.net
Lavrencak
J
J
Us-Krasovec
M
M
D000328:Adult; D004247:DNA / Q000737:chemistry*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000737:chemistry*; D006801:Humans; D019044:Image Cytometry; D008596:Menotropins / Q000627:therapeutic use; D010062:Ovulation Induction; D011003:Ploidies
D008596:Menotropins; D004247:DNA