Identification and quantification of hydroxamic acids in maize seedling root tissue and impact on western corn rootworm (Coleoptera: Chrysomelidae) larval development.
Hydroxamic acid content was analyzed in the root tissue of four maize, Zea mays L., lines using high-performance liquid chromatography (HPLC) and related to western corn rootworm, Diabrotica virgifera virgifera LeConte, larval development and survivorship. Maize lines evaluated included Mp710 (PI 596627), MpSWCB-4, (PI 550498), Sc213 (PI 548792), and Dk580 (DeKalb commercial hybrid). Maize plants from each line were grown in test tubes containing a transparent agarose gel medium in a growth chamber. After 8 d of growth, root tissue of each line was harvested and hydroxamic acid content analyzed using HPLC. Three hydroxamic acids, 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), 6-methoxybenzoxazolinone (MBOA), and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA), were identified in the maize roots tested. DIMBOA concentration was quantified and ranged from 246.37 +/- 70.53 micrograms to 91.84 +/- 49.82 micrograms DIMBOA per gram of root tissue. No significant difference was found among lines in D. v. virgifera larval development and survivorship.
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Department of Entomology, University of Nebraska-Lincoln 68583-0816, USA.
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D000818:Animals; D048588:Benzoxazines; D001583:Benzoxazoles / Q000032:analysis; D001517:Coleoptera / Q000254:growth & development*; D006877:Hydroxamic Acids / Q000032:analysis*; D007814:Larva / Q000254:growth & development; D010078:Oxazines / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry* / Q000502:physiology
D048588:Benzoxazines; D001583:Benzoxazoles; D006877:Hydroxamic Acids; D010078:Oxazines; C104846:2,4-dihydroxy-1,4-benzoxazin-3-one; C031896:6-methoxybenzoxazolinone; C050733:2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one