title: Susceptibility of an avian pneumovirus isolated from Minnesota turkeys to physical and chemical agents.
abstract: Survival characteristics of a Minnesota avian pneumovirus (APV) isolated from a turkey nasoturbinate, propagated in tissue culture, and exposed to various physiochemical treatments were determined. These characteristics included survivability under various conditions. Specifically, APV was viable at temperatures of -70 C and -20 C for over 26 wk, 4 C for less than 12 wk, 20 C for less than 4 wk, 37 C for 48 hr, and 50 C for less than 6 hr. In addition, APV survived 12 freeze/thaw cycles with no loss of activity. With a variable pH for 1 hr, the titer was unaffected between the levels of pH 5 and 9. Several disinfectants, including quaternary ammonia, ethanol, iodophor, a phenol derivative, a biguanide, and bleach, were all effective in reducing the viability of the virus. After 7 days of drying at room temperature, the APV remained viable and was recovered on cell culture.
authors.affiliation: University of Minnesota, College of Veterinary Medicine, St. Paul 55108, USA.
authors.forename: E
authors.initials: E
authors.lastname: Townsend
authors.forename: D A
authors.initials: DA
authors.lastname: Halvorson
authors.forename: K V
authors.initials: KV
authors.lastname: Nagaraja
authors.forename: D P
authors.initials: DP
authors.lastname: Shaw
mesh_terms: D000818:Animals; D004202:Disinfectants; D006863:Hydrogen-Ion Concentration; D018111:Pneumovirus / Q000187:drug effects / Q000302:isolation & purification*; D013696:Temperature; D014420:Turbinates / Q000821:virology; D014422:Turkeys / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods / Q000662:veterinary
chemical_list: D004202:Disinfectants