title: Auxin biosynthesis in maize kernels.
abstract: Auxin biosynthesis was analyzed in a maize (Zea mays) kernel culture system in which the seeds develop under physiological conditions similar to the in vivo situation. This system was modified for precursor feeding experiments. Tryptophan (Trp) is efficiently incorporated into indole-3-acetic acid (IAA) with retention of the 3, 3' bond. Conversion of Trp to IAA is not competed by indole. Labeling with the general precursors [U-(13)C(6)]glucose and [1, 2-(13)C(2)]acetate followed by retrobiosynthetic analysis strongly suggest that Trp-dependent IAA synthesis is the predominant route for auxin biosynthesis in the maize kernel. The synthesis of IAA from indole glycerol phosphate and IAA formation via condensation of indole with an acetyl-coenzyme A or phosphoenolpyruvate derived metabolite can be excluded.
authors.affiliation: Lehrstuhl für Genetik, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Glawischnig
authors.forename: A
authors.initials: A
authors.lastname: Tomas
authors.forename: W
authors.initials: W
authors.lastname: Eisenreich
authors.forename: P
authors.initials: P
authors.lastname: Spiteller
authors.forename: A
authors.initials: A
authors.lastname: Bacher
authors.forename: A
authors.initials: A
authors.lastname: Gierl
mesh_terms: D000596:Amino Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007210:Indoleacetic Acids / Q000096:biosynthesis* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010937:Plant Growth Regulators / Q000096:biosynthesis / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D014364:Tryptophan