[Effects of leukotriene B4 receptor antagonist on experimental autoimmune uveoretinitis in rats].
OBJECTIVE
We evaluated the potential of leukotriene B4 receptor antagonist (LTB4 RA) as an anti-inflammatory agent on experimental autoimmune uveoretinitis (EAU).


METHODS
LTB4 RA was administered to Lewis rats twice a day on days 0-10, 0-5, and 6-10 after immunization. Rats treated on days 0-10 after immunization were subdivided into three groups according to the dosage of LTB4 RA. The eyes were examined histopathologically, and the expression of CD 45 RC in CD 4+T cells was analyzed.


RESULTS
The inflammatory changes in the eyes of EAU were decreased in all groups treated with LTB4 RA in a dose-dependent manner. The treatment with LTB4 RA on days 0-10 after immunization achieved much higher uveitis suppression. The infiltration into eye tissues by neutrophils and lymphocytes was decreased by treatment with LTB4 RA. In treated groups, the CD 45 RChigh subset decreased in the induction phase of EAU as compared with the untreated group.


CONCLUSIONS
The suppressive mechanisms of LTB4 RA on EAU may be dependent on suppression of the activation of neutrophils and CD 4+T cells.
Toriyama
S
S
Department of Ophthalmology, Yokohama City University School of Medicine, Japan.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D001327:Autoimmune Diseases / Q000188:drug therapy*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005123:Eye / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D017493:Leukocyte Common Antigens; D020024:Leukotriene Antagonists / Q000008:administration & dosage*; D008297:Male; D020556:Neutrophil Infiltration; D051381:Rats; D011917:Rats, Inbred Lew; D018102:Receptors, Leukotriene B4 / Q000037:antagonists & inhibitors*; D012173:Retinitis / Q000188:drug therapy*; D016176:T-Lymphocyte Subsets; D014605:Uveitis / Q000188:drug therapy*
D000893:Anti-Inflammatory Agents; D020024:Leukotriene Antagonists; D018102:Receptors, Leukotriene B4; D017493:Leukocyte Common Antigens