Determination of clopidol residues in chicken tissues by high-performance liquid chromatography-mass spectrometry.
A high-performance liquid chromatographic-mass spectrometric (HPLC-MS) method has been developed for determination of clopidol residues in chicken tissues. Samples are extracted with acetonitrile. The extracts are cleaned up on an alumina column followed by an anion-exchange column. The clopidol is separated on a column (150 cmx4.6 mm) of Intertsil by using acetonitrile-water (20:80) as mobile phase. The clopidol was qualitatively identified by molecule mass and determined quantitatively by selected ion monitoring mode at 190 m/z. The recoveries with RSDs ranged from 91.6+/-10.1 to 97.3+/-5.7 at 0.010 to 10.0 mg/kg by spiking three matrices (chicken muscle, liver, and kidney). The limit of detection was 0.005 mg/kg, and the limit of quantification was 0.010 mg/kg.
Pang
G F
GF
Qinhuangdao Import & Export Commodity Inspection Bureau, P.C., China.
Cao
Y Z
YZ
Fan
C L
CL
Zhang
J J
JJ
Li
X M
XM
Wang
C
C
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003007:Clopidol / Q000032:analysis*; D003049:Coccidiostats / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D003049:Coccidiostats; D003007:Clopidol