ELISA for bovine interleukin-1 receptor antagonist and its application to mastitic sera and whey.
A sandwich ELISA for the bovine IL-1 receptor antagonist (bIL-1ra) was developed using recombinant (r) bIL-1ra produced by Escherichia coli, anti-rbIL-1ra rabbit IgG, its biotinylated one and avidin-peroxidase. This ELISA system enabled detection of rbIL-1ra at a concentration of more than 2 ng/ml. This ELISA was applied to quantitation of bIL-1ra in sera and whey of mastitic and healthy cows. The results indicate that although IL-1ra levels in healthy and mastitic sera and whey were comparable, serum IL-1ra/IL-1beta ratio of euthanized cows was significantly lower than that of the recovered.
Yamanaka
H
H
Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Hisaeda
K
K
Hagiwara
K
K
Kirisawa
R
R
Iwai
H
H
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle / Q000276:immunology* / Q000382:microbiology; D004756:Enterobacteriaceae Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D004927:Escherichia coli Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D005260:Female; D006090:Gram-Negative Bacteria / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D008414:Mastitis, Bovine / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D008894:Milk Proteins / Q000032:analysis; D008970:Molecular Weight; D011233:Precipitin Tests / Q000662:veterinary; D011512:Proteus Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology; D017472:Receptors, Interleukin-1 / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000097:blood; D000067816:Whey Proteins
D008894:Milk Proteins; D017472:Receptors, Interleukin-1; D000067816:Whey Proteins