Identification of microsatellite length polymorphism by a polymerase chain reaction-based assay at IL-1beta gene locus in cancer cells.
It has long been considered that genetic instability is an integral component of human neoplasia, however the frequency of microsatellite instability in human melanoma and non-melanoma skin cancer was less evidenced. We report here an alteration at the complex dinucleotide repeat (TC)n sequence in the interleukin 1-beta gene locus by polymerase chain reaction (PCR)-based assay in mouse melanoma as well as in various cancer cells, which may provide a simple, rapid, non-radioactive screening assay for cancer detection.
Chakraborty
A K
AK
Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520, USA. ashok.chakraborty@yale.edu
D000818:Animals; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D007375:Interleukin-1 / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000235:genetics; D008546:Melanoma, Experimental / Q000235:genetics*; D051379:Mice; D008811:Mice, Inbred DBA; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*
D004273:DNA, Neoplasm; D007375:Interleukin-1; D004247:DNA