Structure of an acidic O-specific polysaccharide of the bacterium Providencia alcalifaciens O7.
An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O7 and purified by gel chromatography followed by anion-exchange chromatography. On the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1H- and 13C-NMR spectroscopy, including two-dimensional 1H,1H homonuclear and H-detected 1H,13C heteronuclear correlation spectroscopy and nuclear Overhauser effect spectroscopy (NOESY), the following structure of the linear tetrasaccharide repeating unit of the polysaccharide was established: [figure], where Rhap2Ac is 2-O-acetylrhamnopyranose.
Bystrova
O V
OV
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia. knirel@ioc.ac.ru
Zatonskii
G V
GV
Borisova
S A
SA
Kocharova
N A
NA
Shashkov
A S
AS
Knirel
Y A
YA
Kholodkova
E V
EV
Stanislavskii
E S
ES
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D011532:Providencia / Q000737:chemistry*
D019081:O Antigens