title: Mediators of neuronal activation in the rat brainstem following intestinal anaphylaxis.
abstract: Brainstem neurones become activated following intestinal antigen challenge but the signalling mechanisms have not been resolved. Our aim was to determine the extent of brainstem activation after intestinal anaphylaxis induced by chicken egg albumin (EA). An increase in Fos-positive neurones in the nucleus tractus solitarius (nTS) was observed following EA (P<0.05). Fos-expression was decreased following pretreatment with pyrilamine and ondansetron i.p. and to a similar extent when both antagonists were administered together (all P<0.05 vs. control). Indomethacin had no effect on Fos-expression after antigen challenge. 5-HT and histamine but not prostanoids, released following intestinal anaphylaxis, induce nTS activation via histamine H(1)- and 5-HT(3) receptors. Information on the intestinal inflammatory status is relayed centrally and may play a role in reflexes and behavioural responses to activation of the immune system.
authors.affiliation: University Hospital Tübingen, Department of General Surgery, Tübingen, Germany.
authors.forename: M E
authors.initials: ME
authors.lastname: Kreis
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: T T
authors.initials: TT
authors.lastname: Zittel
authors.forename: J
authors.initials: J
authors.lastname: Glatzle
authors.forename: D
authors.initials: D
authors.lastname: Grundy
mesh_terms: D000707:Anaphylaxis / Q000378:metabolism / Q000503:physiopathology*; D000818:Animals; D001933:Brain Stem / Q000378:metabolism / Q000502:physiology* / Q000503:physiopathology; D002645:Chickens; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007150:Immunohistochemistry; D007213:Indomethacin / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000294:innervation* / Q000503:physiopathology*; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010047:Ovalbumin / Q000633:toxicity; D016760:Proto-Oncogene Proteins c-fos / Q000502:physiology; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011969:Receptors, Histamine H1 / Q000502:physiology; D017552:Solitary Nucleus / Q000166:cytology / Q000187:drug effects / Q000502:physiology
chemical_list: D006634:Histamine H1 Antagonists; D016760:Proto-Oncogene Proteins c-fos; D011969:Receptors, Histamine H1; D010047:Ovalbumin; D011738:Pyrilamine; D007213:Indomethacin