Adaptive immunity against Listeria monocytogenes in the absence of type I tumor necrosis factor receptor p55.
Tumor necrosis factor (TNF) and the type I TNF receptor (TNFRI), p55, are critical for resistance against primary infections with the intracellular bacterial pathogen Listeria monocytogenes. Importantly, however, susceptibility to primary listeriosis in cytokine-deficient mice does not preclude the development or expression of effective adaptive immunity against virulent L. monocytogenes. We used TNFRI(-/-) mice to study adaptive antilisterial immunity in the absence of interactions between TNF and TNFRI. Our experiments indicate that TNFRI(-/-) mice survive and clear high-dose challenges with an attenuated strain of L. monocytogenes that is incapable of cell-to-cell spread. Furthermore, TNFRI(-/-) mice immunized with attenuated L. monocytogenes go on to develop potent adaptive immunity to subsequent high-dose challenges with virulent L. monocytogenes. Interestingly, CD8(+) T-cell depletion in vivo inhibits immunity to L. monocytogenes in the spleen but not in the liver of TNFRI(-/-) mice. The adaptive immune response in these animals is characterized by activation of listeriolysin O-specific CD8(+) T cells, which are capable of transferring antilisterial immunity to naive wild-type C57BL/6 host mice. These experiments demonstrate the development and expression of potent CD8(+) T-cell-mediated antilisterial immunity in the absence of TNFRI.
White
D W
DW
Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USA.
Badovinac
V P
VP
Fan
X
X
Harty
J T
JT
D000220:Adaptation, Biological; D019264:Adoptive Transfer; D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D006360:Heat-Shock Proteins / Q000276:immunology; D006460:Hemolysin Proteins; D007111:Immunity, Cellular; D008088:Listeriosis / Q000276:immunology* / Q000401:mortality; D008099:Liver / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics*; D047888:Receptors, Tumor Necrosis Factor, Type I; D013154:Spleen / Q000276:immunology; D014611:Vaccination
D015703:Antigens, CD; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; C038705:hlyA protein, Listeria monocytogenes