The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems
Kinetics of the triple bioluminescent enzyme system: alcohol dehydrogenase--NADH:FMN-oxidoreductase--luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD+/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring.
Kudryasheva
NS
Institute of Biophysics, Russian Academy of Sciences Siberian Branch, Krasnoyarsk. kudr@bbl.krasnoyarsk.su
Kudinova
IY
Esimbekova
EN
Kratasyuk
VA
Stom
DI