Apical gene transfer into quiescent human and canine polarized intestinal epithelial cells by lentivirus vectors.
Intestinal epithelial cells secrete a protective luminal mucus barrier inhibiting viral gene transfer. Quiescent, polarized monolayers of primary epithelial cells from dog gallbladder and human colon are efficiently transduced through the apical mucus side by lentivirus vectors, suggesting their application to intestinal gene therapy.
Seppen
J
J
Department of Pediatrics, School of Medicine, University of Washington, Washington, USA.
Barry
S C
SC
Klinkspoor
J H
JH
Katen
L J
LJ
Lee
S P
SP
Garcia
J V
JV
Osborne
W R
WR
D000818:Animals; D018938:Caco-2 Cells; D002460:Cell Line; D016764:Cell Polarity; D004285:Dogs; D004847:Epithelial Cells / Q000821:virology; D005704:Gallbladder / Q000166:cytology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000821:virology*; D016086:Lentivirus / Q000235:genetics*; D008562:Membrane Glycoproteins; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D014161:Transduction, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics
C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins