title: Sexing sand fly pupae (Diptera: psychodidade: phlebotominae).
abstract: We describe a technique to separate male and female pupae of sand flies. This has reduced the labour to separate flies after emergence and also allows the isolation of unmated adults for behavioural and physiological studies.
authors.affiliation: Laboratório de Leishmanioses, Centro de Pesquisas René Rachou, Fundação do Instituto Oswaldo Cruz, Belo Horizonte, Brasil.
authors.forename: B G
authors.initials: BG
authors.lastname: Brazil
authors.forename: R P
authors.initials: RP
authors.lastname: Brazil
mesh_terms: D000818:Animals; D005260:Female; D008297:Male; D010691:Phlebotomus / Q000166:cytology / Q000502:physiology*; D011679:Pupa / Q000502:physiology; D012727:Sex Characteristics