Positional cloning in Arabidopsis. Why it feels good to have a genome initiative working for you.
Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.
Lukowitz
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Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305, USA.
Gillmor
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Scheible
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D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018745:Genome, Plant; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic