title: Aberrant trafficking of the B cell receptor Ig-alpha beta subunit in a B lymphoma cell line.
abstract: The B cell Ag receptor (BCR) has two important functions: first, it binds and takes up Ag for presentation to T lymphocytes; and second, it transmits signals that regulate B cell development. Normal expression of the BCR requires the association of the Ag binding subunit, membrane IgM (mIgM), with the signaling component, the Ig-alpha beta heterodimer. After assembly in the endoplasmic reticulum, the intact BCR travels through the secretory pathway to the cell surface. In this paper, we report two variants of the B lymphoma cell lines, WEHI 279 and WEHI 231, that have both lost the ability to express mu heavy chain and consequently do not express mIgM. However, these variants do express the Ig-alpha beta heterodimer. In one variant, WEHI 279*, the Ig-alpha beta remained trapped intracellularly in the absence of mIgM. The other variant, 303.1.5.LM, expressed an aberrantly glycosylated Ig-alpha beta on the cell surface that was capable of signaling after cross-linking with anti-Ig-beta Abs. Further characterization uncovered a point mutation in the 303.1.5.LM mb1 gene that would change a proline for a leucine in the extracellular domain of Ig-alpha. The 303.1.5.LM Ig-alpha beta could not associate with a wild-type mIgM after mu heavy chain was reconstituted by DNA transfection. Thus, this mutation could define a region of the Ig-alpha polypeptide that is important for recognition by the endoplasmic reticulum quality control system, for association with glycosylating enzymes, and for the association of Ig-alpha beta subunits with mIgM subunits to create a complete BCR complex.
authors.affiliation: Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Condon
authors.forename: S L
authors.initials: SL
authors.lastname: Hourihane
authors.forename: M
authors.initials: M
authors.lastname: Dang-Lawson
authors.forename: J
authors.initials: J
authors.lastname: Escribano
authors.forename: L
authors.initials: L
authors.lastname: Matsuuchi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport / Q000235:genetics / Q000276:immunology; D051925:CD79 Antigens; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D006224:Cricetinae; D006596:Hexosaminidases / Q000378:metabolism; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000378:metabolism; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis; D016393:Lymphoma, B-Cell / Q000276:immunology* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D051379:Mice; D009154:Mutation; D011947:Receptors, Antigen, B-Cell / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D051925:CD79 Antigens; C497882:Cd79a protein, mouse; C497884:Cd79b protein, mouse; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D008562:Membrane Glycoproteins; D011947:Receptors, Antigen, B-Cell; D006596:Hexosaminidases