title: Cell surface-associated enolase in Actinobacillus actinomycetemcomitans.
abstract: Cell surface-associated materials of Actinobacillus actinomycetemcomitans were extracted by a short incubation of the cell suspension in a Tris-buffered saline in the presence and absence of a restriction enzyme, EcoRI. The supernatants (which we termed EcoRI extract and surface extract, respectively) contained a number of extracellularly released proteins. Of these proteins, four major proteins were identified by N-terminal sequencing to be the 34 and 39 kDa outer membrane proteins, the GroEL-like protein, and a 47 kDa protein homologous to Haemophilus influenzae enolase. Enolase activity was found in the extracts and its relative amount of activity in the EcoRI extract from a culture of the mid-exponential growth phase was estimated as 5.7% of total enzyme activity. In contrast, the relative amount of activity of another cytosolic enzyme, lactate dehydrogenase, was extremely low in the extracts and also in the culture supernatant. These results suggest the external localization of enolase in this bacterium.
authors.affiliation: Department of Periodontology and Endontology, Okayama University Dental School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hara
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Ohashi
authors.forename: S
authors.initials: S
authors.lastname: Takashiba
authors.forename: Y
authors.initials: Y
authors.lastname: Murayama
authors.forename: K
authors.initials: K
authors.lastname: Fukui
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry / Q000201:enzymology*; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D018834:Chaperonin 60 / Q000032:analysis; D015246:Deoxyribonuclease EcoRI; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018834:Chaperonin 60; D008565:Membrane Proteins; D015246:Deoxyribonuclease EcoRI; D010751:Phosphopyruvate Hydratase