title: Positional cloning in Arabidopsis. Why it feels good to have a genome initiative working for you.
abstract: Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.
authors.affiliation: Department of Plant Biology, Carnegie Institution of Washington, 260 Panama Street, Stanford, California 94305, USA.
authors.forename: W
authors.initials: W
authors.lastname: Lukowitz
authors.forename: C S
authors.initials: CS
authors.lastname: Gillmor
authors.forename: W R
authors.initials: WR
authors.lastname: Scheible
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018745:Genome, Plant; D020161:Physical Chromosome Mapping; D011110:Polymorphism, Genetic