title: Phylogenetic studies of tospoviruses (family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs.
abstract: Analysis of the intergenic region (IGR) of S and M RNAs of tospoviruses (Family Bunyaviridae) indicated their heterogeneity both in length and sequence. In general, IGRs of M RNA were shorter in length compared to the IGRs of their respective S RNA species. Percent identity among the S RNA IGR sequences of distinct tospovirus species varied from 42 to 57%, whereas it was 79 to 99% among isolates of the same species. Similarly, when IGRs of M RNAs were compared, there was higher sequence identity among isolates of the same tospovirus species (84 to 98%) than among distinct tospovirus species (46 to 59%). Percent nucleotide identities and maximum likelihood trees of IGR sequences of S and M RNAs indicated that their sequence divergence is similar to that of nucleocapsid gene at inter and intra-species levels. This is the first detailed sequence analysis of IGRs of S and M RNAs of known tospoviruses.
authors.affiliation: Department of Entomology, University of Georgia, Tifton, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Pappu
authors.forename: A I
authors.initials: AI
authors.lastname: Bhat
authors.forename: H R
authors.initials: HR
authors.lastname: Pappu
authors.forename: C M
authors.initials: CM
authors.lastname: Deom
authors.forename: A K
authors.initials: AK
authors.lastname: Culbreath
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D016679:Genome, Viral; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D013045:Species Specificity; D017865:Tospovirus / Q000145:classification* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012367:RNA, Viral