Determination of four nitroimidazole residues in poultry meat by liquid chromatography-mass spectrometry.
A multi-residue liquid chromatography-mass spectrometry (LC-MS) assay method is described for the determination of four nitroimidazoles in poultry muscle. The extraction procedure is based on liquid-liquid extraction with ethyl acetate followed by an evaporation step. A deuterated internal standard is used. The LC separation was made on a C18 bonded silica column with an aqueous formic acid (0.2%) solution-methanol-acetonitrile (81:13:6) mobile phase. Following electrospray ionization, the protonated molecular ion [M+H]+ is obtained for each compound. Monitoring several ions for each nitroimidazole provides the specificity required for confirmatory assay. Validation of the method was performed to estimate linearity, intra-day and inter-day repeatability, accuracy and detection limit. The present method is capable of identifying nitroimidazole residues in muscle at levels below 5 microg/kg.
Hurtaud-Pessel
D
D
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire des Médicaments Vétérinaires, La Haute Marche, Javené, Fougères, France.
Delépine
B
B
Laurentie
M
M
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D009593:Nitroimidazoles / Q000032:analysis*; D011200:Poultry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000890:Anti-Infective Agents; D009593:Nitroimidazoles