Identification of the UV-responsive sequence in the human tissue plasminogen activator gene.
Functional analysis of regulatory elements in the human tissue plasminogen activator (tPA) gene showed that its promoter (-119/+169) is activated by UV irradiation in HeLa cells. We demonstrated here that the AP-2 like CCCCACCC sequence is involved in the UV-mediated activation and Sp1 binds to the sequence.
Fujiwara
J
J
AISIN Cosmos R&D CO., Ltd., Kazusa Incubation Center, Yana Kisarazu, Chiba, Japan.
Fujii
M
M
Shimoda
M
M
Ayusawa
D
D
D001483:Base Sequence; D004249:DNA Damage; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006367:HeLa Cells / Q000528:radiation effects; D006801:Humans; D008545:Melanoma / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011993:Recombinant Fusion Proteins; D020218:Response Elements / Q000235:genetics / Q000528:radiation effects*; D016329:Sp1 Transcription Factor / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D010959:Tissue Plasminogen Activator