Cytokine production by in vitro processed and unprocessed haematopoietic cells.
The studies described here demonstrate that in vitro processing of cells before extraction of RNA has a major effect on the number and type of cytokine transcripts present within MDS and leukemia cells. Transcripts for GM-CSF, a cytokine whose production by leukemia cells is believed to play an important role in the pathogenesis of leukemia, was not detectable in 12/13 unprocessed AML specimens, in 12/12 MDS specimens, or in 7/7 CML specimens but once detected in many specimens after processing. These data strongly suggest that leukemia cell production of GMCSF rarely occurs in vivo.
Bi
S
S
Rush Cancer Institute, Departments of Medicine, Immunology & Microbiology, Rush-Presbyterian-St Luke's Medical Center, Rush Medical College, Chicago, IL 60612, USA. sbi@rush.edu
Gao
X
X
Devemy
E
E
Chopra
H
H
Venugopal
P
P
Raza
A
A
Preisler
H D
HD
D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D018929:Cell Culture Techniques; D002469:Cell Separation; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000276:immunology; D013048:Specimen Handling; D020298:U937 Cells
D016207:Cytokines