Interaction of viper venom serine peptidases with thrombin receptors on human platelets.
The serine peptidases, thrombocytin and PA-BJ, isolated from the venom of Bothrops atrox and Bothrops jararaca, respectively, induce platelet aggregation and granule secretion without clotting fibrinogen. The specific platelet aggregation activity of each enzyme was about 15 times lower than that of thrombin. This activity was blocked by monoclonal antibodies recognizing protease activated receptor 1 (PAR1) and by heparin, but not by hirudin nor thrombomodulin. Both enzymes induced calcium mobilization in platelets and desensitized platelets to the action of thrombin and the SFLLRN peptide. We compared the effect of thrombin, PA-BJ, and thrombocytin on the degradation of the soluble N-terminal domain of the PAR1 receptor. The major cleavage site by thrombin and both viper enzymes was Arg41-Ser42. In addition, a rapid cleavage of the peptide bond at Arg46-Asn47 by the viper enzymes was observed, resulting in the inactivation of the tethered ligand. PA-BJ and thrombocytin both cleaved at 41-42 and 46-47 peptide bonds, and fragment 42-103 disappeared rapidly. Both viper enzymes caused calcium mobilization in fibroblasts transfected with PAR4 and desensitized these cells to the thrombin action. In conclusion, both PAR1 and PAR4 mediate the effect of viper venom serine peptidases on platelets.
Santos
B F
BF
Instituto Butantam, Sao Paulo, Brazil.
Serrano
S M
SM
Kuliopulos
A
A
Niewiarowski
S
S
D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology; D011485:Protein Binding; D018179:Receptors, Thrombin / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology; D014757:Viper Venoms / Q000201:enzymology*
D010446:Peptide Fragments; D018179:Receptors, Thrombin; D014757:Viper Venoms; C082835:thrombin receptor peptide (42-47); D012697:Serine Endopeptidases; D013917:Thrombin