title: Digital light-directed synthesis. A microarray platform that permits rapid reaction optimization on a combinatorial basis.
abstract: Solution reactions using photogenerated reagents (Gao, X.; Yu, P.; LeProust, E.; Sonigo, L.; Pellois, J. P.; Zhang, H. J. Am. Chem. Soc. 1998, 120, 12698) are a potentially powerful means for combinatorial parallel synthesis of addressable molecular microarrays. In this report, we demonstrate that this chemistry permits combinatorial screening of reaction conditions on a microarray platform. Using this method of optimization and our reaction apparatus, efficient photogenerated acids and reaction conditions suitable for removal of the acid labile protection group on 5'-O of nucleotides are identified in a short period of time. The chemistry platform demonstrated opens new avenues for rapid, simultaneous investigation of multiple reactions using different reagents and reaction parameters directly on a solid support (e.g., a glass plate). The combinatorial screening method described may be extended to include general organic reactions employing photogenerated and conventional reagents as well as a microarray reaction device. This should be especially valuable for efficient synthesis of addressable organic compound libraries.
authors.affiliation: Department of Chemistry, University of Houston, Texas 77204-5641, USA.
authors.forename: E
authors.initials: E
authors.lastname: LeProust
authors.forename: J P
authors.initials: JP
authors.lastname: Pellois
authors.forename: P
authors.initials: P
authors.lastname: Yu
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.forename: O
authors.initials: O
authors.lastname: Srivannavit
authors.forename: E
authors.initials: E
authors.lastname: Gulari
authors.forename: X
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002625:Chemistry, Organic / Q000379:methods*; D015195:Drug Design; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010777:Photochemistry; D012996:Solutions; D013329:Structure-Activity Relationship
chemical_list: D009711:Nucleotides; D012996:Solutions