Development of phosphine reagents for the high-performance liquid chromatographic-fluorometric determination of lipid hydroperoxides.
Phosphine reagents were designed and synthesized as a new type of fluorescent reagents for the determination of lipid hydroperoxides in foodstuff and biological materials. All phosphine reagents prepared had no fluorescence but their oxides, which were produced by the reaction of the phosphines with hydroperoxides, had strong fluorescence. Among the phosphine reagents prepared, diphenyl-1-pyrenylphosphine had the most suitable properties as a fluorescent reagent and was successfully applied to the determination of hydroperoxides by batch, flow injection and HPLC post-column methods.
Akasaka
K
K
Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Ohrui
H
H
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D008054:Lipid Peroxides / Q000032:analysis*; D010720:Phosphines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D007202:Indicators and Reagents; D008054:Lipid Peroxides; D010720:Phosphines; C044646:phosphine