title: Modulation of CD95 in leukemia and lymphoma cells by retinoids.
abstract: Retinoids are highly active for the treatment of acute promyelocytic leukemia, but have effects on many different types of normal and transformed cells. CD95 is a member of the TNF-receptor superfamily and can mediate apoptosis. In this study, we screened a series of 7 myeloid and 12 lymphoid cell lines for the expression of CD95 and for the induction of CD95 by retinoids. A differential response was observed: in 3/7 myeloid cell lines a significant down-regulation of CD95 happened which correlated with the induction of differentiation. A significant induction of CD95 was observed in 4/ 12 lymphoid lines (2 HHV8+ cell lines, one Burkitt line and another lymphoma line). The modulation of CD95 was both time- and dose- dependent (maximum reached after 3-5 days of culture with 10-6 M retinoid). The 3 retinoid compounds studied (all-trans retinoic acid, 9-cis retinoic acid and 13-cis retinoic acid) had comparable activity. Taken together, we describe a new mode of induction of CD95 in lymphoma cells. More work is needed to define the functional consequences of these changes in CD95 surface expression.
authors.affiliation: IV. Medizinische Abteilung, Städt. Krankenhaus Harlaching, Sanatoriumsplatz, D-81545 München, Germany. rmunker@hotmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Munker
authors.forename: P
authors.initials: P
authors.lastname: Koeffler
authors.forename: R
authors.initials: R
authors.lastname: Haas
authors.forename: W
authors.initials: W
authors.lastname: Wilmanns
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D012176:Retinoids / Q000494:pharmacology* / Q000627:therapeutic use; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000096:biosynthesis*
chemical_list: D012176:Retinoids; D019014:fas Receptor