Hepatic alpha(2u)-globulin mRNA levels and diethylstilbestrol-associated testicular atrophy in rats.
Biosynthesis of alpha(2u)-Globulin (alpha(2u)-g) is under multihormonal regulation. In this study, we investigated histopathologic changes in the testis and hepatic alpha(2u)-g messenger ribonucleic acid (mRNA) levels in male rats after administration of the potent estrogen diethylstilbestrol (DES) at 0. 01, 0.1, or 1 mg/kg/day by gavage for 14 days. DES treatment decreased hepatic alpha(2u)-g mRNA levels in a dose-dependent manner accompanied by atrophic histopathologic changes in the testis. In addition, alpha(2u)-g mRNA levels were lowest in animals with the most marked testicular changes. Hepatic alpha(2u)-g mRNA may be a useful biomarker for the evaluation of endocrine disruption in male rats.
Takeyoshi
M
M
Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 3-822, Ishii-machi, Hita-shi, 8770061, Oita, Japan. takeyoshi-masahiro@hita.cerij.or.jp
Anai
S
S
Shinoda
K
K
D000510:Alpha-Globulins / Q000235:genetics*; D000818:Animals; D001284:Atrophy; D015415:Biomarkers; D004054:Diethylstilbestrol / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000187:drug effects* / Q000473:pathology
D000510:Alpha-Globulins; D015415:Biomarkers; D012333:RNA, Messenger; C013803:alpha 2u globulin; D012313:RNA; D004054:Diethylstilbestrol