title: Low erucic acid canola oil does not induce heart triglyceride accumulation in neonatal pigs fed formula.
abstract: Canola oil is not approved for use in infant formula largely because of concerns over possible accumulation of triglyceride in heart as a result of the small amounts of erucic acid (22:1n-9) in the oil. Therefore, the concentration and composition of heart triglyceride were determined in piglets fed from birth for 10 (n = 4-6) or 18 (n = 6) d with formula containing about 50% energy fat as 100% canola oil (0.5% 22:1n-9) or 100% soybean oil, or 26% canola oil or soy oil (blend) with palm, high-oleic sunflower and coconut oil, providing amounts of 16:0 and 18:1 closer to milk, or a mix of soy, high-oleic sunflower and flaxseed oils with C16 and C18 fatty acids similar to canola oil but without 22:1. Biochemical analysis found no differences in heart triglyceride concentrations among the groups at 10 or 18 d. Assessment of heart triglycerides using Oil Red O staining in select treatments confirmed no differences between 10-d-old piglets fed formula with 100% canola oil (n = 4), 100% soy oil (n = 4), or the soy oil blend (n = 2). Levels of 22:1n-9 in heart triglyceride and phospholipid, however, were higher (P<0.01) in piglets fed 100% canola oil or the canola oil blend, with higher levels found in triglycerides compared with phospholipids. The modest accumulation of 22:1n-9 associated with feeding canola oil was not associated with biochemical evidence of heart triglyceride accumulation at 10 and 18 d.
authors.affiliation: Department of Paediatrics, University of British Columbia, Vancouver, Canada.
authors.forename: T J
authors.initials: TJ
authors.lastname: Green
authors.forename: S M
authors.initials: SM
authors.lastname: Innis
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D004883:Erucic Acids / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D000074262:Rapeseed Oil; D013552:Swine; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D004883:Erucic Acids; D005229:Fatty Acids, Monounsaturated; D000074262:Rapeseed Oil; D014280:Triglycerides; C049811:erucic acid