title: [Ribozyme which specifically cleave 12-lipoxygenase mRNA: in vitro activity and its dynamics].
abstract: The ribozyme gene which specifically cleaved 12-lipoxygenase mRNA was designed, synthesized and cloned according to hammer-head ribozyme model. A 25-bp-long segment of 12-lipoxygenase mRNA was chemically synthesized as substrate, which was incubated with transcribed ribozyme RNA to detect its cleavage activity in vitro. The results showed that the ribozyme had a relatively high cleavage activity against 12-lipoxygenase mRNA segment at 37 degrees C. It's kcat value and Km value was 0.083/min and 1300 nmol/L respectively. The ribozyme had a very high activity at 50 degrees C and its kcat value was 0.31/min.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing.
authors.forename: C H
authors.initials: CH
authors.lastname: Liu
authors.forename: L Q
authors.initials: LQ
authors.lastname: Sun
authors.forename: P
authors.initials: P
authors.lastname: Tien
mesh_terms: D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics*; D003001:Cloning, Molecular; D016337:RNA, Catalytic / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D016337:RNA, Catalytic; D012333:RNA, Messenger; D001092:Arachidonate 12-Lipoxygenase