[Rapid progression of passaged mouse hepatocarcinoma associated with the loss of cell polarity].
Two transplantable differentiated mouse hepatocarcinomas were obtained in B6 D2 F1 mice from primary tumors induced by initiation (NDEA)-promotion (phenobarbital) protocol. Both HC were slowly growing with time from passage to passage of 5-7 months (s-HC). A fast-growing variant with time between passages of 2-3 weeks appeared in one mouse on the third passage (f-HC). The three HC strains constantly retained their phenotype. The s-HCs were characterized by prominent cell polarity according to organization of the cytoskeleton and distribution of domain-specific membrane-associated markers. Cell polarity was completely destroyed in the fast-growing variant. Cell adhesion in the latter tumor was very low. An in vitro growing strain of f-HC was easily obtained enabling experimental study of regulation of progression in HCs.
Engel'gardt
N V
NV
N. N. Blokhin Russian Cancer Research Center, Moscow.
Kudriavtseva
E I
EI
Morozova
O V
OV
Rudinskaia
T D
TD
Turusov
V S
VS
D000818:Animals; D002455:Cell Division; D016764:Cell Polarity; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D008297:Male; D051379:Mice; D014407:Tumor Cells, Cultured