Early indication of effects of puromycin aminonucleoside using a fluorimetric assay of 2-aminoacridone-derivatized carbohydrates in urine.
This paper describes a novel noninvasive method to study the changes in free carbohydrates excreted in urine as a result of toxicity in the rat induced by the administration of puromycin aminonucleoside (PAN). Urine samples were collected for 24 h prior to dosing and at 8, 24, and 32 h postdosing. For each sample, free carbohydrates were extracted from the urine using a graphitized carbon column and then labeled with 2-aminoacridone (2-AMAC) prior to analysis by hydrophilic interaction liquid chromatography (HILC). Dramatic changes were seen in the profile of the carbohydrates at the 8- and 24-h time points. These changes in carbohydrate profiles may be useful as early indicators of toxicity.
Charlwood
J
J
SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow, Essex, CM19 5AW, United Kingdom.
Birrell
H
H
Tolson
D
D
Connelly
J
J
Camilleri
P
P
D000609:Aminoacridines / Q000737:chemistry / Q000652:urine*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity; D002241:Carbohydrates / Q000737:chemistry / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005470:Fluorometry / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D011692:Puromycin Aminonucleoside / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000609:Aminoacridines; D000903:Antibiotics, Antineoplastic; D002241:Carbohydrates; D009844:Oligosaccharides; C072515:2-aminoacridone; D011692:Puromycin Aminonucleoside