Biological and morphological effects on the brain after exposure of rats to a 1439 MHz TDMA field.
We investigated the effects of exposure to a 1439 MHz TDMA (Time Division Multiple Access) field, as used in cellular phones, on the permeability of the blood-brain barrier (BBB), on the morphological changes of the brain, and on body-mass fluctuations. Male Sprague-Dawley (SD) rats were divided into three groups of eight rats each. The rats in the EM(+) group, which had their heads arrayed in a circle near the central antenna of an exposure system, were exposed to a 1439 MHz field for one hour a day. The rats in EM(-) group were also in the exposure system, however, without high-frequency electromagnetic wave (HF-EMW) exposure. The animals in the control group were neither placed in the system nor exposed to HF-EMWs. The exposure period was two or four weeks. The energy dose rate peaked at 2 W/kg in the brain; the average over the whole body was 0.25 W/kg. The changes in the permeability of BBB were investigated by Evans blue injection method and by immunostaining of serum albumin. HF-EMWs had no effect on the permeability of BBB. The morphological changes in the cerebellum were investigated by assessing the degeneration of Purkinje cells and the cell concentration in the granular layer. No significant changes were observed in the groups of rats exposed to HF-EMWs for two or four weeks. Averaged body masses were not affected by HF-EMWs exposure. In conclusion, a 1439 MHz TDMA field did not induce observable changes in the permeability of the BBB, morphological changes in the cerebellums, or body mass changes in rats, as evaluated by the conventional methods.
Tsurita
G
G
Department of Surgical Oncology, Graduate School of Medicine, The University of Tokyo, Japan. turita-1su@h.u-tokyo.ac.jp
Nagawa
H
H
Ueno
S
S
Watanabe
S
S
Taki
M
M
D000818:Animals; D001812:Blood-Brain Barrier; D001835:Body Weight; D001921:Brain / Q000473:pathology* / Q000503:physiopathology*; D002531:Cerebellum / Q000473:pathology; D004574:Electromagnetic Fields / Q000009:adverse effects*; D008297:Male; D008872:Microwaves / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013689:Telephone