title: Dopamine D1 and adenosine A1 receptors form functionally interacting heteromeric complexes.
abstract: The possible molecular basis for the previously described antagonistic interactions between adenosine A(1) receptors (A(1)R) and dopamine D(1) receptors (D(1)R) in the brain have been studied in mouse fibroblast Ltk(-) cells cotransfected with human A(1)R and D(1)R cDNAs or with human A(1)R and dopamine D(2) receptor (long-form) (D(2)R) cDNAs and in cortical neurons in culture. A(1)R and D(1)R, but not A(1)R and D(2)R, were found to coimmunoprecipitate in cotransfected fibroblasts. This selective A(1)R/D(1)R heteromerization disappeared after pretreatment with the D(1)R agonist, but not after combined pretreatment with D(1)R and A(1)R agonists. A high degree of A(1)R and D(1)R colocalization, demonstrated in double immunofluorescence experiments with confocal laser microscopy, was found in both cotransfected fibroblast cells and cortical neurons in culture. On the other hand, a low degree of A(1)R and D(2)R colocalization was observed in cotransfected fibroblasts. Pretreatment with the A(1)R agonist caused coclustering (coaggregation) of A(1)R and D(1)R, which was blocked by combined pretreatment with the D(1)R and A(1)R agonists in both fibroblast cells and in cortical neurons in culture. Combined pretreatment with D(1)R and A(1)R agonists, but not with either one alone, substantially reduced the D(1)R agonist-induced accumulation of cAMP. The A(1)R/D(1)R heteromerization may be one molecular basis for the demonstrated antagonistic modulation of A(1)R of D(1)R receptor signaling in the brain. The persistence of A(1)R/D(1)R heteromerization seems to be essential for the blockade of A(1)R agonist-induced A(1)R/D(1)R coclustering and for the desensitization of the D(1)R agonist-induced cAMP accumulation seen on combined pretreatment with D(1)R and A(1)R agonists, which indicates a potential role of A(1)R/D(1)R heteromers also in desensitization mechanisms and receptor trafficking.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Barcelona, 08028 Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Ginés
authors.forename: J
authors.initials: J
authors.lastname: Hillion
authors.forename: M
authors.initials: M
authors.lastname: Torvinen
authors.forename: S
authors.initials: S
authors.lastname: Le Crom
authors.forename: V
authors.initials: V
authors.lastname: Casadó
authors.forename: E I
authors.initials: EI
authors.lastname: Canela
authors.forename: S
authors.initials: S
authors.lastname: Rondin
authors.forename: J Y
authors.initials: JY
authors.lastname: Lew
authors.forename: S
authors.initials: S
authors.lastname: Watson
authors.forename: M
authors.initials: M
authors.lastname: Zoli
authors.forename: L F
authors.initials: LF
authors.lastname: Agnati
authors.forename: P
authors.initials: P
authors.lastname: Verniera
authors.forename: C
authors.initials: C
authors.lastname: Lluis
authors.forename: S
authors.initials: S
authors.lastname: Ferré
authors.forename: K
authors.initials: K
authors.lastname: Fuxe
authors.forename: R
authors.initials: R
authors.lastname: Franco
mesh_terms: D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D000242:Cyclic AMP / Q000378:metabolism; D019281:Dimerization; D018491:Dopamine Agonists / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011233:Precipitin Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D018047:Receptors, Purinergic P1 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D018491:Dopamine Agonists; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D018047:Receptors, Purinergic P1; C047235:N-(1-methyl-2-phenylethyl)adenosine; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D000242:Cyclic AMP; D000241:Adenosine