Spectrum of autoantibodies against a dynamin-related protein, dymple.
OBJECTIVE
To characterize the clinical features of patients with autoantibodies against dymple, a dynamin-related protein.


METHODS
Serum samples from 281 patients with rheumatic diseases were examined. The characteristics of antidymple and antibody-reactive determinants were investigated by immunoblotting with the recombinant dymple antigen, including its deletion mutants, and by immunofluorescence studies with affinity-purified serum.


RESULTS
Five serum samples (2%) were found to have antidymple. All of these patients were male, and 4 of them had interstitial pneumonitis. Their sera were considered to mainly recognize the N-terminus of dymple, which contains GTP-binding motifs.


CONCLUSIONS
Dymple, which joins the cytoplasmic autoantigens, could be a marker for a newly recognized subset of connective tissue diseases.
Muro
Y
Y
Department of Dermatology, Nagoya University School of Medicine, Japan.
Kamimoto
T
T
Tomita
Y
Y
Hagiwara
M
M
D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers; D002460:Cell Line; D018076:DNA, Complementary / Q000032:analysis; D034281:Dynamins; D005260:Female; D005455:Fluorescent Antibody Technique; D020558:GTP Phosphohydrolases / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D017563:Lung Diseases, Interstitial / Q000097:blood / Q000276:immunology; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D012216:Rheumatic Diseases / Q000097:blood / Q000201:enzymology / Q000276:immunology*
D001323:Autoantibodies; D015415:Biomarkers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D020558:GTP Phosphohydrolases; D034281:Dynamins