title: Adhesion of inactivated probiotic strains to intestinal mucus.
abstract: It has been suggested that probiotics should be viable in order to elicit beneficial health effects. Inactivation of probiotics has been suggested to interfere with the binding to the mucosa and thereby with the immune modulating activity of probiotics. The effect of different inactivation methods on the mucus adhesion of nine probiotic strains was studied. Inactivation by heat or gamma-irradiation generally decreased the adhesive abilities. However, heat treatment increased the adhesion of Propionibacterium freudenreichii and gamma-irradiation enhanced the adhesion of Lactobacillus casei Shirota. Inactivation by u.v. was not observed to modulate the adhesion of the tested strains and it was concluded to be the most appropriate method for studying non-viable probiotics and preparing control products.
authors.affiliation: Department of Biochemistry and food Chemistry, University of Turku,and Turku, Finland. arthur.ouwehand@utu.fi
authors.forename: A C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: S
authors.initials: S
authors.lastname: Tölkkö
authors.forename: J
authors.initials: J
authors.lastname: Kulmala
authors.forename: S
authors.initials: S
authors.lastname: Salminen
authors.forename: E
authors.initials: E
authors.lastname: Salminen
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology* / Q000528:radiation effects; D001644:Bifidobacterium / Q000378:metabolism / Q000528:radiation effects; D005243:Feces / Q000737:chemistry; D005720:Gamma Rays; D006361:Heating; D007413:Intestinal Mucosa / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism / Q000528:radiation effects; D009093:Mucus / Q000737:chemistry / Q000378:metabolism*; D019936:Probiotics / Q000378:metabolism* / Q000528:radiation effects; D011424:Propionibacterium / Q000378:metabolism / Q000528:radiation effects; D012440:Saccharomyces / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays