Stigmatella aurantiaca Sg a15 carries genes encoding type I and type II 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases: involvement of a type II synthase in aurachin biosynthesis.
3-Deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthases catalyse the first step of the shikimate pathway. Two unrelated DAHP synthase types have been described in plants and bacteria. Two type II (aroA(A2) and aroA(A5)) and one type I DAHP synthase gene (aroA001) were identified from the myxobacterium Stigmatella aurantiaca Sg a15. Inactivation of aroA(A5) leads to a mutant that is impaired in the biosynthesis of aurachins, which are electron transport inhibitors and contain an anthranilate moiety. Feeding of anthranilic acid to the mutant culture restores production of aurachins. Inactivation of aroA(A2) and aroA001 does not impair production of aurachins or other known secondary metabolites of S. aurantiaca Sg a15.
Silakowski
B
B
Gesellschaft für Biotechnologische Forschung (GBF), Department NBi/MX, Braunschweig, Germany.
Kunze
B
B
Müller
R
R
D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003360:Cosmids; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D015363:Quinolones / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020606:Stigmatella aurantiaca / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development
D015363:Quinolones; D010723:3-Deoxy-7-Phosphoheptulonate Synthase