Involvement of tyrosine kinase in citrate-stimulated aldosterone production in bovine glomerulosa cells.
The present study was designed to assess whether citrate stimulates aldosterone production by isolated bovine adrenal glomerulosa cells in vitro. When the cells were incubated with graded concentrations of citrate up to 4.0 mM, basal aldosterone production was significantly elevated, with a gradual reduction of extracellular ionized calcium concentration. Without citrate, however, adding increasing amounts of calcium chloride to a calcium-free medium did not reproduce the citrate's effect on basal aldosterone production. Genistein, an inhibitor of tyrosine kinases, inhibited the citrate (4 mM)-induced aldosterone production in a dose-dependent manner, with 89.8% of inhibition at a concentration of 10 microM. When the cells were exposed to citrate (4 mM) for 5, 10, and 30 min, tyrosine in Mr 105,000 endogenous protein was dominantly phosphorylated. This study demonstrates for the first time that citrate stimulates aldosterone production in bovine adrenal glomerulosa cells in vitro and also suggests a crucial involvement of protein tyrosine kinase in the steroidogenic action of citrate in the cells.
Kigoshi
T
T
Division of Endocrinology, Department of Internal Medicine, Kanazawa Medical University, Ishikawa 920-02, Japan.
Imaizumi
N
N
Yoshida
J
J
Nakagawa
A
A
Nakano
S
S
Nishio
M
M
Uchida
K
K
D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002417:Cattle; D019343:Citric Acid / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D000451:Mineralocorticoid Receptor Antagonists / Q000494:pharmacology; D009994:Osmolar Concentration; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*; D014443:Tyrosine / Q000378:metabolism; D015384:Zona Glomerulosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D004791:Enzyme Inhibitors; D000451:Mineralocorticoid Receptor Antagonists; D019343:Citric Acid; D014443:Tyrosine; D000450:Aldosterone; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D002122:Calcium Chloride