title: [Nanobacteria. An experimental neo-lithogenesis model].
abstract: OBJECTIVE
  To study the phenomenon of biomineralization in an experimental model of lithogenesis by percutaneous renal punction without laparotomy and no antibiotics.
  
  
  RESULTS
  The study comprised 4 Brown-Norway rats. Nanobacteria inoculum (X, 2X and 4X) was administered to three rats and the remaining one was used as control. The analytical and radiological findings showing the development of obstructive pyelocaliceal lithiasis in the kidneys of rats no. 2 and 3 are presented.
  
  
  CONCLUSIONS
  Translumbar percutaneous renal puncture has permitted performing laparotoy without antibiotic coverage, which was the main difficulty of the experimental model of lithogenesis. Nanobacteria were cultured successfully, but not without difficulty, and formation of calculi in the rat pyelocaliceal system was achieved. This experimental model will provide further insight into lithogenesis and will allow us to find the answers to some of the many questions concerning this condition that remain.
authors.affiliation: Servicio de Urología, Hospital Ramón y Cajal, Universidad de Alcalá, Madrid, España.
authors.forename: E
authors.initials: E
authors.lastname: García Cuerpo
authors.forename: E
authors.initials: E
authors.lastname: Olavi Kajander
authors.forename: N
authors.initials: N
authors.lastname: Ciftçioglu
authors.forename: F
authors.initials: F
authors.lastname: Lovaco Castellano
authors.forename: C
authors.initials: C
authors.lastname: Correa
authors.forename: J
authors.initials: J
authors.lastname: González
authors.forename: F
authors.initials: F
authors.lastname: Mampaso
authors.forename: F
authors.initials: F
authors.lastname: Liaño
authors.forename: E
authors.initials: E
authors.lastname: García de Gabiola
authors.forename: A
authors.initials: A
authors.lastname: Escudero Barrilero
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D003460:Crystallization; D004195:Disease Models, Animal; D007669:Kidney Calculi / Q000209:etiology* / Q000382:microbiology*; D051381:Rats; D011914:Rats, Inbred BN