The gene of Ca2+-binding protein regucalcin is highly conserved in vertebrate species.
Regucalcin is a Ca2+-binding protein, which plays a regulatory role in liver cell functions related to Ca2+. In this study we have cloned cDNA for regucalcin from rabbit, bovine, chicken and toad livers by using rapid amplification of cDNA ends (RACE) method. The nucleotide and amino acid sequences of them are compared with published human, rat and mouse sequences. Comparison analysis revealed that the nucleotide sequences of regucalcin from seven vertebrate species were highly conserved in their coding region. The overall regucalcin proteins in these species consisted of 299 amino acids, and they had 69.9-91.3% identity. Furthermore, phylogenetic analysis showed that regucalcin in seven species appears to form a single cluster. This study demonstrates a great conservation of the regucalcin genes throughout evolution.
Misawa
H
H
Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
Yamaguchi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D002265:Carboxylic Ester Hydrolases; D002417:Cattle; D002645:Chickens; D018076:DNA, Complementary; D019143:Evolution, Molecular; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015238:Sulfotransferases; D014981:Xenopus
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C513323:RGN protein, human; C513324:Rgn protein, mouse; D012313:RNA; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; D002265:Carboxylic Ester Hydrolases; C513325:Rgn protein, rat