title: Interferon-tau modulates phorbol ester-induced production of prostaglandin and expression of cyclooxygenase-2 and phospholipase-A(2) from bovine endometrial cells.
abstract: Antiluteolytic actions of bovine interferon-tau (bIFN-tau) require suppression of prostaglandin F(2 alpha) (PGF(2 alpha)) production. Our objective was to test whether bIFN-tau could block PGF(2 alpha) production and synthesis of phospholipase A(2) (PLA(2)) and cyclooxygenase-2 (COX-2) enzymes induced by a protein kinase C (PKC) stimulator (phorbol 12,13 dibutyrate; PDBu). Bovine endometrial epithelial (BEND) cells were treated with PDBu in the presence or absence of bIFN-tau. Medium samples were analyzed for concentrations of PGF(2 alpha), whole-cell extracts were analyzed for abundance of PLA(2) and COX-2 by immunoblotting, and RNA extracts were examined for steady-state levels of COX-2 mRNA by Northern blotting. The PDBu stimulated production of PGF(2 alpha) between 3 and 12 h, levels of COX-2 mRNA by 3 h and protein expression of COX-2 and PLA(2) by 6 and 12 h, respectively. Added concomitantly with PDBu, bIFN-tau suppressed PGF(2 alpha) production, steady-state levels of COX-2 mRNA, and expression of COX-2 and PLA(2) proteins. Added after a 3-h stimulation with PDBu alone, bIFN-tau suppressed PGF(2 alpha) production after 1 h. Bovine IFN-tau inhibited intracellular mechanisms responsible for PGF(2 alpha) production in BEND cells, and this could be through both cytosolic and nuclear actions.
authors.affiliation: Department of Dairy and Poultry Sciences, University of Florida, Gainesville, Florida 32611, USA.
authors.forename: M
authors.initials: M
authors.lastname: Binelli
authors.forename: A
authors.initials: A
authors.lastname: Guzeloglu
authors.forename: L
authors.initials: L
authors.lastname: Badinga
authors.forename: D R
authors.initials: DR
authors.lastname: Arnold
authors.forename: J
authors.initials: J
authors.lastname: Sirois
authors.forename: T R
authors.initials: TR
authors.lastname: Hansen
authors.forename: W W
authors.initials: WW
authors.lastname: Thatcher
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D051546:Cyclooxygenase 2; D015237:Dinoprost / Q000096:biosynthesis; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D015151:Immunoblotting; D007370:Interferon Type I / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010741:Phospholipases A / Q000235:genetics*; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D011453:Prostaglandins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D007370:Interferon Type I; D007527:Isoenzymes; D011257:Pregnancy Proteins; D011453:Prostaglandins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C020947:interferon tau; D015240:Phorbol 12,13-Dibutyrate; D015237:Dinoprost; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A