Oleoyl-estrone lowers the body weight of both ob/ob and db/db mice.
Homozygous obese db/db (BKS-Lepr(db) and ob/ob (B6-Lep(ob)) mice were treated for 14 days with a continuous infusion of a fat emulsion (controls) or loaded with oleoyl-estrone at doses of 12.5 and 50 nmol/g x d using surgically inserted osmotic minipumps. Treatment with oleoyl-estrone resulted in a marked decrease in body weight in both strains, compared with the unchecked growth of controls. In db/db mice, plasma urea and insulin, as well as liver lipid decreased with treatment. In ob/ob mice, the effect on insulin was more marked, in parallel with higher plasma lipids pointing to increased fat mobilisation. The results suggest that oleoyl-estrone effects on body fat reserves and insulin resistance are not mediated by leptin, since ob/ob mice lack this hormone and in the db/db it is present but cannot induce effects because of defective leptin receptors; in both cases oleoyl-estrone treatment lowers body weight.
Grasa
M M
MM
Centre de Recerca en Nutrició i Ciència dels Aliments, Universitat de Barcelona, Spain.
Vilà
R
R
Esteve
M
M
Cabot
C
C
Fernandez-López
J A
JA
Remesar
X
X
Alemany
M
M
D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects*; D004970:Estrone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D051379:Mice; D008820:Mice, Obese; D009829:Oleic Acids / Q000494:pharmacology*
D019440:Anti-Obesity Agents; D007328:Insulin; D008055:Lipids; D009829:Oleic Acids; D004970:Estrone; C108709:oleoyl-estrone