Role of ribosome recycling factor (RRF) in translational coupling.
RNA phage GA coat and lysis protein expression are translationally coupled through an overlapping termination and initiation codon UAAUG. Essential for this coupling are the proximity of the termination codon of the upstream coat gene to the initiation codon of the lysis gene (either a <3 nucleotide separation or physical closeness through a possible hairpin structure) but not the Shine-Dalgarno sequence. This suggests that the ribosomes completing the coat gene translation are exclusively responsible for translation of the lysis gene. Inactivation of ribosome recycling factor (RRF), which normally releases ribosomes at the termination codon, did not influence the expression of the reporter gene fused to the lysis gene. This suggests the possibility that RRF may not release ribosomes from the junction UAAUG. However, RRF is essential for correct ribosomal recognition of the AUG codon as the initiation site for the lysis gene.
Inokuchi
Y
Y
Department of Bioscience, Teikyo University, Utsunomiya 320-8551, Yakult Pharmaceutical Inc. Co. Tokyo, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan.
Hirashima
A
A
Sekine
Y
Y
Janosi
L
L
Kaji
A
A
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000235:genetics; D018387:Codon, Initiator / Q000235:genetics; D018388:Codon, Terminator / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000821:virology; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D020539:Sequence Analysis, Protein; D017384:Sequence Deletion; D013379:Substrate Specificity; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D018387:Codon, Initiator; D018388:Codon, Terminator; D011506:Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014764:Viral Proteins; C087267:ribosome releasing factor