Susceptibility of an avian pneumovirus isolated from Minnesota turkeys to physical and chemical agents.
Survival characteristics of a Minnesota avian pneumovirus (APV) isolated from a turkey nasoturbinate, propagated in tissue culture, and exposed to various physiochemical treatments were determined. These characteristics included survivability under various conditions. Specifically, APV was viable at temperatures of -70 C and -20 C for over 26 wk, 4 C for less than 12 wk, 20 C for less than 4 wk, 37 C for 48 hr, and 50 C for less than 6 hr. In addition, APV survived 12 freeze/thaw cycles with no loss of activity. With a variable pH for 1 hr, the titer was unaffected between the levels of pH 5 and 9. Several disinfectants, including quaternary ammonia, ethanol, iodophor, a phenol derivative, a biguanide, and bleach, were all effective in reducing the viability of the virus. After 7 days of drying at room temperature, the APV remained viable and was recovered on cell culture.
Townsend
E
E
University of Minnesota, College of Veterinary Medicine, St. Paul 55108, USA.
Halvorson
D A
DA
Nagaraja
K V
KV
Shaw
D P
DP
D000818:Animals; D004202:Disinfectants; D006863:Hydrogen-Ion Concentration; D018111:Pneumovirus / Q000187:drug effects / Q000302:isolation & purification*; D013696:Temperature; D014420:Turbinates / Q000821:virology; D014422:Turkeys / Q000821:virology*; D014776:Virus Cultivation / Q000379:methods / Q000662:veterinary
D004202:Disinfectants