New biosensor for superoxide radical used to evidence molecules of biomedical and pharmaceutical interest having radical scavenging properties.
A superoxide dismutase biosensor was used to determine the antioxidant properties of scavenger molecules and the antiradical activity of healthy and diseased human kidney tissues; this biosensor is based on the use of the enzyme superoxide dismutase (SOD), which is physically entrapped in a kappa-carrageenan gel membrane, and of a transducer consisting of an amperometric hydrogen peroxide electrode. Several compounds with scavenging properties were tested, including some commercial drugs.
Campanella
L
L
Department of Chemistry, La Sapienza University of Rome, Italy. campanellal@axrma.uniroma.it
Favero
G
G
Persi
L
L
Tomassetti
M
M
D015374:Biosensing Techniques; D002351:Carrageenan; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide; D007668:Kidney / Q000378:metabolism; D007674:Kidney Diseases / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
D004800:Enzymes, Immobilized; D016166:Free Radical Scavengers; D013481:Superoxides; D002351:Carrageenan; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase