title: Colocalization of dihydropyridine and ryanodine receptors in neonate rabbit heart using confocal microscopy.
abstract: Because of undeveloped T tubules and sparse sarcoplasmic reticulum, Ca(2+)-induced Ca(2+) release (CICR) may not be the major mechanism providing contractile Ca(2+) in the neonatal heart. Spatial association of dihydropyridine receptors (DHPRs) and ryanodine receptors (RyRs), a key factor for CICR, was examined in isolated neonatal rabbit ventricular myocytes aged 3-20 days by double-labeling immunofluorescence and confocal microscopy. We found a significant increase (P < 0.0005) in the degree of colocalization of DHPR and RyR during development. The number of voxels containing DHPR that also contained RyR in the 3-day-old group (62 +/- 1.8%) was significantly lower than in the other age groups (76 +/- 1.3 in 6-day old, 75 +/- 1.2 in 10-day old, and 79 +/- 0.9% in 20-day old). The number of voxels containing RyR that also contained DHPR was significantly higher in the 20-day-old group (17 +/- 0.5%) compared with the other age groups (10 +/- 0.7 in 3-day old, 11 +/- 0.6 in 6-day old, and 11 +/- 0.5% in 10-day old). During this period, the pattern of colocalization changed from mostly peripheral to mostly internal couplings. Our results provide a structural basis for the diminished prominence of CICR in neonatal heart.
authors.affiliation: Cardiac Membrane Research Laboratory, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Sedarat
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.forename: E D
authors.initials: ED
authors.lastname: Moore
authors.forename: G F
authors.initials: GF
authors.lastname: Tibbits
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D020746:Calcium Channels, L-Type / Q000032:analysis*; D002469:Cell Separation / Q000379:methods; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006352:Heart Ventricles; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods; D009206:Myocardium / Q000166:cytology*; D011817:Rabbits; D019837:Ryanodine Receptor Calcium Release Channel / Q000032:analysis*; D014909:Wheat Germ Agglutinins
chemical_list: D000911:Antibodies, Monoclonal; D020746:Calcium Channels, L-Type; D019837:Ryanodine Receptor Calcium Release Channel; D014909:Wheat Germ Agglutinins