Isolation of a frog lectin using polylysine to reduce non-specific interactions with heparin binding ligand.
Polylysine is a polycation used in histology to glue tissue sections to glass slides. It was successfully used to eliminate positively charged proteins from heparin-agarose beds applied for affinity chromatography facilitating the preparative steps in the isolation and purification of heparin binding lectins from the ovary of the frog Leptodactylus occellatus. The addition pretreatment of agarose-heparin beds with polylysine increased more than 3-fold the hemagglutinating capacity of eluted lectin.
Bonaterra
G A
GA
Centro de Microscopía Electrónica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Aoki
A
A
D000818:Animals; D001001:Anura; D001665:Binding Sites / Q000187:drug effects* / Q000502:physiology*; D002846:Chromatography, Affinity; D006493:Heparin / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification*; D011107:Polylysine / Q000737:chemistry*
D037102:Lectins; D011107:Polylysine; D006493:Heparin