A matrix attachment region is located upstream from the high-molecular-weight glutenin gene Bx7 in wheat (Triticum aestivum L.).
A 2.2-kb nucleotide sequence rich in AT, located upstream from the Bx7 allele of the high-molecular-weight glutenin Glu-B1 locus in wheat (Triticum aestivum cv. Glenlea) was cloned following amplification by PCR. The 5' region of this sequence contains motifs typically found in matrix attachment regions (MARs) in other plants. We have shown that part of the 2.2-kb DNA binds to wheat nuclear matrix (NM) in vitro, at least as strongly as a known MAR (Adh1) from maize suggesting that there is a MAR upstream of Bx7. This MAR is approximately 800 bases in length running from -750 to -1560 bases, relative to the start codon. Although the MAR is associated with a tissue-specific gene and is beside a strong tissue-specific promoter, the MAR sequence did not lead to tissue-specific expression of the beta-glucuronidase marker gene under the control of the rice actin promoter in various tissues. Presence of the MAR was only slightly beneficial with respect to expression levels, which were not greatly altered in transient expression assays in various wheat tissues although a slight increase in the number of foci was observed in leaves, which have low transformation efficiencies.
Rampitsch
C
C
Agriculture and Agri-Food Canada, Cereal Research Centre, Winnipeg, MB.
Jordan
M C
MC
Cloutier
S
S
D002843:Chromatin / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018745:Genome, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D005983:Glutens / Q000031:analogs & derivatives* / Q000235:genetics; D015530:Nuclear Matrix / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000235:genetics; D014908:Triticum / Q000235:genetics*
D002843:Chromatin; D018744:DNA, Plant; D005983:Glutens; D005966:Glucuronidase; C037302:glutenin