Ordering three microsatellites on porcine chromosome 12 by single sperm typing.
Three microsatellite loci on porcine chromosome 12 were ordered by single sperm typing to expand the limited genetic map of this region. Individual sperm cells from a Chinese indigenous Qingping boar triply heterozygous at SW874, SW1350 and SW1553 were amplified using PEP and heminesting primer design at each locus. Analysis of the sperm typing data by the SPERM.FOR program showed that the most likely order was SW1553-SW1350-SW874.
Zhao
S
S
Laboratory of Molecular Biology and Animal Breeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, PR China.
Li
K
K
Yu
M
M
Peng
Z
Z
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary; D002875:Chromosomes; D006239:Haplotypes; D008297:Male; D018895:Microsatellite Repeats; D013094:Spermatozoa / Q000737:chemistry*; D013552:Swine / Q000235:genetics*