Expression and translocation of Drosophila nuclear encoded cytochrome b(5) proteins to mitochondria.
DNA sequence studies of cytochrome b(5) (Cyt-b) genes from Drosophila melanogaster and Drosophila virilis predict that the Drosophila Cyt-b proteins are extremely hydrophobic and have at least eight potential transmembrane spanning domains. Primary protein sequence analysis also predicts that the Cyt-b proteins have mitochondrial targeting sequences and they contain sites for potential post-translational modification similar to other cytochrome proteins. We report the characterization of the cytochrome b(5) proteins from Drosophila melanogaster and Drosophila virilis. We have used a Drosophila cytochrome b(5) specific antibody to demonstrate that cytochrome b(5) proteins are expressed in muscle-containing tissues in the fly. We also provide evidence that the nuclear encoded cytochrome b(5) protein that contains a mitochondrial targeting sequence is translocated to mitochondria.
Kula
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ME
Department of Biology, Case Western Reserve University, Cleveland, 44106, Ohio, USA.
Rozek
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CE
D000818:Animals; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D015786:Cytochromes b5 / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D013347:Subcellular Fractions
D015786:Cytochromes b5