Dose dependency of prednisolone tertiary-butylacetate (PTBA) treatment on the establishment and site predilection of Echinococcus multilocularis in an alternative definitive host model using Mongolian gerbil (Meriones unguiculatus).
Mongolian gerbils (Meriones unguiculatus) were orally inoculated with 10,000 protoscoleces of Echinococcus multilocularis per head after being divided into five groups (A E). Each group was dosed with prednisolone tertiary-butylacetate (PTBA) as follows: A, 0 mg; B, 0.5 mg; C, 2 mg; D, 5 mg; and E, 10 mg/head. All animals were injected subcutaneously with control solvent or PTBA every other day from 6 days pre- to 6 days post-infection. Autopsy was performed at 7 days post-infection. Doses of PTBA and the number of worms recovered showed a positive correlation (r = 0.929, P < 0.0001). In groups A, B and C, the predilection site of the worms in the small intestine could not be determined, while in group D the worms were found more in the anterior part. In group E, the predilection site was the anterior part, followed by the middle and the posterior parts of the small intestine (Fisher's test: P < 0.01). The number of worms recovered from the anterior and the middle part of the small intestine also correlated positively with PTBA dose (anterior part: r = 0.930, P < 0.0001, middle part: r = 0.917, P < 0.0001). All groups of the PTBA-treated animals showed significant loss of weight compared to the nontreated animals (P < 0.01).
Matsuo
K
K
Laboratory of Parasitology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. kmatsuo@vetmed.hokudai.ac.jp
Nonaka
N
N
Oku
Y
Y
Kamiya
M
M
D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004443:Echinococcosis / Q000469:parasitology*; D005260:Female; D005849:Gerbillinae / Q000469:parasitology*; D006790:Host-Parasite Interactions; D007421:Intestine, Small / Q000469:parasitology*; D008297:Male; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D015431:Weight Loss
C009021:prednisolone tebutate; D011239:Prednisolone