Hydra biological detection of biologically active peptides in rat cerebrospinal fluid.
The Hydra bioassay system utilizes a tentacle ball formation (TBF), a component of the feeding response of hydra, elicited by S-methyl-glutathione. TBF is modulated by many biologically active peptides in a specific way to individual peptides, and is useful in investigating biologically active peptides in a complex biological sample. We applied the hydra bioassay to explore a possible biologically active substance responsible for the decrease in the motor activity of the mice. The suppression of the CSF obtained from rats after exhaustive exercise was marked lower than that of sedentary rats. Addition of transforming growth factor-beta (TGF-beta), which is the only substance known to nullify TBF, to CSF of the sedentary rat reproduced this change in the suppression of the TBF. This system is useful to screen active peptides in small amounts of biological samples containing very low concentrations of peptides.
Manabe
Y
Y
Laboratory of Nutrition Chemistry, Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, 606-8502, Kyoto, Japan.
Yamazaki
H
H
Fukuda
C
C
Inoue
K
K
Fushiki
T
T
Hanai
K
K
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001681:Biological Assay / Q000379:methods*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis* / Q000494:pharmacology; D005221:Fatigue / Q000134:cerebrospinal fluid*; D005247:Feeding Behavior / Q000187:drug effects; D006829:Hydra / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D009479:Neuropeptides / Q000134:cerebrospinal fluid* / Q000494:pharmacology; D005082:Physical Exertion; D051381:Rats; D017207:Rats, Sprague-Dawley; D012146:Rest / Q000502:physiology; D014357:Trypsin
D002556:Cerebrospinal Fluid Proteins; D009479:Neuropeptides; D014357:Trypsin