title: Detection of prognostic significant translocations in childhood acute lymphoblastic leukaemia by one-step multiplex reverse transcription polymerase chain reaction.
abstract: The search for chromosomal translocations in de novo cases of childhood acute lymphoblastic leukaemia (ALL) is crucial for the selection of the appropriate therapeutic protocol. In this work, we describe a new method - one-step multiplex reverse transcription polymerase chain reaction (RT-PCR) - to screen for prognostic significant translocations in childhood ALL. Our approach involves a single PCR reaction for the simultaneous detection of the molecular rearrangements resulting from the t(9;22), t(12;21), t(4;11) and t(1;19), with a turnaround time of less than 24 h. This assay proved to be highly sensitive, specific, reproducible and easy to implement in a routine genetics laboratory.
authors.affiliation: Unidade de Oncologia Molecular, Serviço de Genética, Instituto Português de Oncologia, Porto, Portugal.
authors.forename: N
authors.initials: N
authors.lastname: Cerveira
authors.forename: S
authors.initials: S
authors.lastname: Ferreira
authors.forename: S
authors.initials: S
authors.lastname: Dória
authors.forename: I
authors.initials: I
authors.lastname: Veiga
authors.forename: F
authors.initials: F
authors.lastname: Ferreira
authors.forename: J M
authors.initials: JM
authors.lastname: Mariz
authors.forename: M
authors.initials: M
authors.lastname: Marques
authors.forename: S
authors.initials: S
authors.lastname: Castedo
mesh_terms: D002675:Child, Preschool; D002878:Chromosomes, Human, Pair 1; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D002888:Chromosomes, Human, Pair 19; D002891:Chromosomes, Human, Pair 21; D002892:Chromosomes, Human, Pair 22; D002894:Chromosomes, Human, Pair 4; D002899:Chromosomes, Human, Pair 9; D006801:Humans; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000235:genetics*; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014178:Translocation, Genetic