Identification of the site of glucocorticoid action on neutral amino acid transport in superficial nephrons of rat kidney.
Glucocorticoid hormones enhance the reabsorptive capacity of filtered amino acids in rat kidney, as it was shown in previous in vivo clearance experiments. In the present study, the site of glucocorticoid action on neutral amino acid transport in superficial nephrons of rat kidney was investigated using in vivo micropuncture technique. Adult female Wistar rats were treated with dexamethasone (DEX), and fractional excretion of L-glutamine (L-Gln) and L-leucine (L-Leu) were determined and related to inulin after microinfusion into different nephron segments. DEX reduced fractional excretion of both neutral amino acids as a sign of enhanced reabsorptive capacity. The site of main DEX action on L-Leu reabsorption has been localized in the proximal straight tubule. However, in the case of L-Gln, the inhibition of gamma-glutamyltranspeptidase (gamma-GT) by administration of acivicin indicated the importance of this brush border enzyme in reduced L-Gln excretion. DEX enhanced gamma-GT activity by tubular acidification. It can be presumed a DEX-inducible transport system for neutral amino acids mainly localized in proximal straight tubules of rat kidney.
Schwertfeger
M
M
Institute of Pharmacology and Toxicology, Friedrich Schiller University of Jena, Federal Republic of Germany. MASCH@mti-n.uni-jena.de
Roskos
M
M
Fleck
C
C
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000378:metabolism* / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D005973:Glutamine / Q000652:urine; D007555:Isoxazoles / Q000494:pharmacology; D007672:Kidney Cortex / Q000187:drug effects; D007930:Leucine / Q000652:urine; D009399:Nephrons / Q000187:drug effects / Q000378:metabolism*; D011677:Punctures; D051381:Rats; D017208:Rats, Wistar; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism
D000596:Amino Acids; D004791:Enzyme Inhibitors; D005938:Glucocorticoids; D007555:Isoxazoles; D005973:Glutamine; D018698:Glutamic Acid; D003907:Dexamethasone; D005723:gamma-Glutamyltransferase; D007930:Leucine; C006303:acivicin