Dendritic cells permit identification of genes encoding MHC class II-restricted epitopes of transplantation antigens.
Minor or histocompatibility (H) antigens are recognized by CD4+ and CD8+ T lymphocytes as short polymorphic peptides associated with MHC molecules. They are the targets of graft versus host and graft versus leukemia responses following bone marrow transplantation between HLA-identical siblings. Several genes encoding class I-restricted minor H epitopes have been identified, but approaches used for these have proved difficult to adapt for cloning class II-restricted minor H genes. We have combined the unique antigen-presenting properties of dendritic cells and high levels of episomal expression following transfection of COS cells to identify a Y chromosome gene encoding two HY peptide epitopes, HYAb and HYEk.
Scott
D
D
Transplantation Biology Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom.
Addey
C
C
Ellis
P
P
James
E
E
Mitchell
M J
MJ
Saut
N
N
Jurcevic
S
S
Simpson
E
E
D000595:Amino Acid Sequence; D000818:Animals; D053487:DEAD-box RNA Helicases; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015852:Eukaryotic Initiation Factor-2 / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics; D006182:H-Y Antigen / Q000096:biosynthesis / Q000235:genetics; D006649:Histocompatibility Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D056466:Histone Demethylases; D007113:Immunity, Innate / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D015778:Minor Histocompatibility Antigens / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010455:Peptides / Q000235:genetics / Q000276:immunology; D011506:Proteins / Q000235:genetics
D053487:DEAD-box RNA Helicases; D018984:Epitopes, T-Lymphocyte; D015852:Eukaryotic Initiation Factor-2; D006183:H-2 Antigens; D006182:H-Y Antigen; D006649:Histocompatibility Antigens; D056466:Histone Demethylases; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D010455:Peptides; D011506:Proteins; C108513:H-2A antigen; C484453:UTY protein, human; C102450:Uty protein, mouse; C490659:Kdm5d protein, mouse; C492034:DDX3Y protein, human; C115600:Ddx3y protein, mouse