title: PCR-based detection of the polymorphism at codon 136 in the ovine prion protein gene.
abstract: In many breeds of sheep, a polymorphism at codon 136 of the prion protein gene has been shown to be strongly associated with the risk of developing scrapie. A single-step procedure for detecting this allelic variation is described here. When performed on a series of animals, the test was in complete agreement with their genotypes as had been previously determined by sequencing. The test is potentially easier and quicker to perform than any of the variety of methods that are currently used for this purpose.
authors.affiliation: Division of Nutritional Biochemistry, School of Biological Sciences, University of Nottingham, Loughborough, Leics., UK.
authors.forename: A K
authors.initials: AK
authors.lastname: Lockley
authors.forename: B D
authors.initials: BD
authors.lastname: Hosie
authors.forename: L
authors.initials: L
authors.lastname: Moore
authors.forename: R
authors.initials: R
authors.lastname: Harling
authors.forename: R G
authors.initials: RG
authors.lastname: Bardsley
mesh_terms: D000818:Animals; D003062:Codon; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics*; D012756:Sheep / Q000235:genetics*
chemical_list: D003062:Codon; D017931:DNA Primers; D011328:Prions