Effect of brimonidine on rabbit trabecular meshwork hyaluronidase activity.
OBJECTIVE
To study the presence of hyaluronidase activity in the rabbit trabecular meshwork and its regulation by brimonidine.


METHODS
A spectrophotometric assay that consists of the assessment of N-acetylhexosamine groups released from hyaluronic acid was used to examine hyaluronidase activity. Cyclic adenosine monophosphate (cAMP) levels were assessed by radioimmunoassay.


RESULTS
Hyaluronidase activity was detected in the rabbit trabecular meshwork. Its optimal activity was in the acid range of pH 3.8. Brimonidine significantly increased trabecular hyaluronidase-specific activity and decreased cAMP accumulation. Yohimbine significantly inhibited the effect of brimonidine on both hyaluronidase activity and cAMP accumulation.


CONCLUSIONS
The finding of endogenous hyaluronidase activity in rabbit trabecular meshwork supports the hypothesis that this tissue can metabolize its own glycosaminoglycan (GAG) products. The present results suggest, however, that the hypotensive effect of brimonidine could be mediated, at least in part, by its ability to increase GAG catabolism, probably through a cAMP-independent mechanism.
Benozzi
J
J
Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Jaliffa
C O
CO
Firpo Lacoste
F
F
Llomovatte
D W
DW
Keller Sarmiento
M I
MI
Rosenstein
R E
RE
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D000068438:Brimonidine Tartrate; D000242:Cyclic AMP / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008297:Male; D011810:Quinoxalines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011817:Rabbits; D011863:Radioimmunoassay; D014129:Trabecular Meshwork / Q000187:drug effects* / Q000201:enzymology; D015016:Yohimbine / Q000494:pharmacology
D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D011810:Quinoxalines; D015016:Yohimbine; D000068438:Brimonidine Tartrate; D000242:Cyclic AMP; D006821:Hyaluronoglucosaminidase