title: Hindbrain serotonin and the rapid induction of sodium appetite.
abstract: Both systemically administered furosemide and isoproterenol produce water intake (i.e., thirst). Curiously, however, in light of the endocrine and hemodynamic effects produced by these treatments, they are remarkably ineffective in eliciting intake of hypertonic saline solutions (i.e., operationally defined as sodium appetite). Recent work indicates that bilateral injections of the serotonin receptor antagonist methysergide into the lateral parabrachial nuclei (LPBN) markedly enhance a preexisting sodium appetite. The present studies establish that a de novo sodium appetite can be induced with LPBN-methysergide treatment under experimental conditions in which only water is typically ingested. The effects of bilateral LPBN injections of methysergide were studied on the intake of water and 0. 3 M NaCl following acute (beginning 1 h after treatment) diuretic (furosemide)-induced sodium and water depletion and following subcutaneous isoproterenol treatment. With vehicle injected into the LPBN, furosemide treatment and isoproterenol injection both caused water drinking but essentially no intake of hypertonic saline. In contrast, bilateral treatment of the LPBN with methysergide induced the intake of 0.3 M NaCl after subcutaneous furosemide and isoproterenol. Water intake induced by subcutaneous furosemide or isoproterenol was not changed by LPBN-methysergide injections. The results indicate that blockade of LPBN-serotonin receptors produces a marked intake of hypertonic NaCl (i.e., a de novo sodium appetite) after furosemide treatment as well as subcutaneous isoproterenol.
authors.affiliation: Departments of Psychology and Pharmacology and the Cardiovascular Center, University of Iowa, Iowa City, Iowa 52242-1407, USA.
authors.forename: J V
authors.initials: JV
authors.lastname: Menani
authors.forename: L A
authors.initials: LA
authors.lastname: De Luca
authors.forename: R L
authors.initials: RL
authors.lastname: Thunhorst
authors.forename: A K
authors.initials: AK
authors.lastname: Johnson
mesh_terms: D000284:Administration, Oral; D000318:Adrenergic beta-Agonists / Q000008:administration & dosage; D000818:Animals; D004232:Diuretics / Q000008:administration & dosage; D004326:Drinking / Q000187:drug effects; D005665:Furosemide / Q000008:administration & dosage; D007279:Injections, Subcutaneous; D007545:Isoproterenol / Q000008:administration & dosage; D008297:Male; D008784:Methysergide / Q000008:administration & dosage; D008845:Microinjections; D051381:Rats; D017207:Rats, Sprague-Dawley; D012249:Rhombencephalon / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D012982:Sodium, Dietary / Q000378:metabolism*
chemical_list: D000318:Adrenergic beta-Agonists; D004232:Diuretics; D012462:Saline Solution, Hypertonic; D012982:Sodium, Dietary; D012701:Serotonin; D005665:Furosemide; D012964:Sodium; D007545:Isoproterenol; D008784:Methysergide