Effect of the sialyl Lewis X (SLe(x)) moiety on splenic accumulation of SLe(x)-carboxymethylpullulan conjugate.
Sialyl Lewis X (SLe(x)), an E-selectin ligand, was conjugated with carboxymethylpullulan (CMPul) and the disposition characteristics of this conjugate after intravenous administration were investigated using mice with ear edema. The concentration of 3H-labeled SLe(x)-CMPul in the spleen was significantly high. When CMPul was modified with a saccharide unable to bind to E-selectin, this splenic accumulation was not observed. The uptake of radiolabeled SLe(x)-CMPul by the spleen was completely inhibited by a 100-fold molar of cold SLe(x)-CMPul but not by a sialyl N-acetyllactosamine-CMPul conjugate (SLN-CMPul). Microautoradiography analyses revealed that SLe(x)-CMPul accumulated in the marginal zone of the spleen.
Horie
K
K
Drug Delivery System Institute, Ltd., Noda, Chiba, Japan.
Sakagami
M
M
Kuramochi
K
K
Ito
T
T
Hamana
H
H
D000818:Animals; D016718:Arachidonic Acid; D019040:E-Selectin / Q000378:metabolism*; D004487:Edema / Q000139:chemically induced / Q000378:metabolism; D005936:Glucans / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics; D000080506:Sialyl Lewis X Antigen; D013154:Spleen / Q000378:metabolism*; D014018:Tissue Distribution
D019040:E-Selectin; D005936:Glucans; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C410955:carboxymethylpullulan; D016718:Arachidonic Acid