Vaccination of mice against schistosoma bovis with a recombinant fatty acid binding protein from Fasciola hepatica.
Two strains of mice (NMRI and C57/BL) were each immunized with a 15kDa recombinant Fasciola hepatica fatty acid binding protein (FABP) (Fh15) and challenged percutaneously with Schistosoma bovis cercariae. C57/BL mice immunized with Fh15 had significant reductions in S. bovis worm burden recoveries (72% reductions over controls). When using NMRI mice, Fh15 in Freund's adjuvant failed to induce significant protection against S. bovis. In C57/BL mice, only antibodies to the IgG2a isotype increased after the second immunization and remained high through 8 weeks of S. bovis infection. This is the first time that a heterologous recombinant molecule from F. hepatica has been used in vaccination against S. bovis, obtaining a significant reduction in the number of worms in C57/BL mice.
Abáné
J L
JL
Lab. Parasitología, Facultad de Farmacia, Universidad de Salamanca, Avda Campo Charro s/n, 37007, Salamanca, Spain.
Oleaga
A
A
Ramajo
V
V
Casanueva
P
P
Arellano
J L
JL
Hillyer
G V
GV
Muro
A
A
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D002352:Carrier Proteins / Q000276:immunology*; D005210:Fasciola hepatica / Q000276:immunology*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D015801:Helminth Proteins / Q000276:immunology; D008099:Liver / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018996:Myelin P2 Protein / Q000276:immunology*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D012547:Schistosoma / Q000276:immunology*; D012552:Schistosomiasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*
D000909:Antibodies, Helminth; D002352:Carrier Proteins; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D015801:Helminth Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; C074842:Fh15 protein, Fasciola hepatica