Protection induced in mice vaccinated with recombinant collagen-binding protein (CnBP) and alpha-toxoid against intramammary infection with Staphylococcus aureus.
Mice vaccinated with a combination of two Staphylococcus aureus antigens consisting of a recombinant collagen-binding protein (CnBP) and alpha-toxoid (alpha-toxoid) were significantly protected from intramammary challenge infection with S. aureus. The average number of bacteria recovered from the glands of mice vaccinated with the combination of CnBP/alpha-toxoid was significantly lower compared to the average number of bacteria recovered from the glands of mice vaccinated with only CnBP or alpha-toxoid or controls (P< or =0.01). Histopathological examination of mammary glands of mice vaccinated with CnBP together with alpha-toxoid showed no pathological changes, whereas glands of mice vaccinated with CnBP or alpha-toxoid alone developed severe mastitis and showed both focal and disseminated necrosis.
Mamo
W
W
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala. wubshet.mamo@astrazeneca.com
Fröman
G
G
Müller
H P
HP
D000818:Animals; D004357:Drug Synergism; D005260:Female; D016023:Integrins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008321:Mammary Glands, Animal / Q000382:microbiology / Q000473:pathology; D008413:Mastitis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice; D011247:Pregnancy; D038981:Receptors, Collagen; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology*; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D013208:Staphylococcal Toxoid / Q000737:chemistry / Q000276:immunology*; D013209:Staphylococcal Vaccines / Q000276:immunology*; D013211:Staphylococcus aureus / Q000737:chemistry
D016023:Integrins; D038981:Receptors, Collagen; D011993:Recombinant Fusion Proteins; D013208:Staphylococcal Toxoid; D013209:Staphylococcal Vaccines