title: Heart mitochondrial respiratory chain complexes are functionally unaffected in heavy ethanol drinkers without cardiomyopathy.
abstract: BACKGROUND
  Harmful effects of chronic ethanol intake on liver mitochondria have been clearly demonstrated; however, mitochondria from skeletal muscle are preserved, and the effect of ethanol on heart mitochondria remains controversial. We assessed individual enzyme activity of mitochondrial respiratory chain (MRC) complexes and membrane oxidative damage of heart mitochondria in active ethanol drinkers before the development of dilated cardiomyopathy.
  
  
  METHODS
  Heart samples were obtained from otherwise healthy organ donor individuals with a sudden death of traumatic or neurological cause in whom hearts could not be used because of absence of matched receptors or size inadequacy. Detailed history of alcohol intake was achieved from the relatives. Citrate synthase activity was spectrophotometrically assayed, as well as absolute (nmol x min(-1) x mg protein(-1)) and relative (corrected by citrate synthase) activities of complex I, II, III, and IV of the MRC. Oxidative damage of myocardium membranes was assessed measuring the degree of lipid peroxidation by fluorescence using cis-parinaric acid as probe.
  
  
  RESULTS
  We included 10 ethanol drinkers (age 53 +/- 13 years, 100% males, mean lifetime intake of 15.6 +/- 7.9 kg ethanol kg body weight(-1)) and 12 controls (age 60 +/- 10 years, 75% males). Mitochondrial content did not differ between the two groups. Absolute enzyme activities for ethanol drinkers and controls were, respectively, 145 +/- 75 and 130 +/- 50 for complex I (p = NS); 399 +/- 193 and 376 +/- 100 for complex II (p = NS); 719 +/- 288 and 714 +/- 308 for complex III (p = NS); and 475 +/- 139 and 570 +/- 160 for complex IV (p = NS). After correcting such activities by citrate synthase activity, we failed again to demonstrate differences between ethanol drinkers and controls. Lipid peroxidation of myocardium membranes was similar in both groups.
  
  
  CONCLUSIONS
  Chronic ethanol drinkers without cardiomyopathy exhibit normal MRC activity in the heart and do not show increased oxidative damage in myocardial membranes.
authors.affiliation: Department of Internal Medicine, Hospital Clínic, August Pi i Sunyer Biomedical Research Institute, Medical School, University of Barcelona, Catalonia, Spain. omiro@clinic.ub.es
authors.forename: O
authors.initials: O
authors.lastname: Miró
authors.forename: J
authors.initials: J
authors.lastname: Robert
authors.forename: J
authors.initials: J
authors.lastname: Casademont
authors.forename: J R
authors.initials: JR
authors.lastname: Alonso
authors.forename: J M
authors.initials: JM
authors.lastname: Nicolás
authors.forename: J
authors.initials: J
authors.lastname: Fernández-Solá
authors.forename: A
authors.initials: A
authors.lastname: Urbano-Márquez
authors.forename: J B
authors.initials: JB
authors.lastname: Hoek
authors.forename: F
authors.initials: F
authors.lastname: Cardellach
mesh_terms: D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000378:metabolism*; D000704:Analysis of Variance; D009202:Cardiomyopathies; D002492:Central Nervous System Depressants / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology*; D014450:Electron Transport Complex III / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol; D014450:Electron Transport Complex III