title: Bacteriostatic action of a novel four-component bioactive glass.
abstract: Bacterial adhesion to biomaterials causing biomaterial-centered infection and poor tissue integration is a problem that limits the extensive use of many biomaterial devices in clinical applications. Bioactive materials are those biomaterials that form a compliant, mechanically stable bond with host tissue. Sol-gel-derived glasses in the three-component system SiO(2)-CaO-P(2)O(5) exhibit bioactivity that is dependent on composition and texture (i.e., specific surface area, pore size, structure, and distribution). The in vitro bioactivity and antibacterial action of a novel sol-gel-derived glass, AgBG, in the system SiO(2)-CaO-P(2)O(5)-Ag(2)O are compared with those of its three-component counterpart, BG. The incorporation of 3-wt % Ag(2)O conferred antimicrobial properties to the glass without compromising its bioactivity. AgBG exhibited a marked bacteriostatic effect on E. coli MG1655 with a minimum inhibiting concentration of 0.2 mg (biomaterial)/mL (culture solution), above which bacterial growth was reduced to 0.01% of that of the control culture. In comparison, BG did not possess antimicrobial properties over the concentration range investigated (0.1-40.0 mg/mL).
authors.affiliation: Department of Materials, Imperial College of Science Technology and Medicine, Prince Consort Road, London, SW7 2BP, United Kingdom. m.bellantone@ic.ac.uk
authors.forename: M
authors.initials: M
authors.lastname: Bellantone
authors.forename: N J
authors.initials: NJ
authors.lastname: Coleman
authors.forename: L L
authors.initials: LL
authors.lastname: Hench
mesh_terms: D000900:Anti-Bacterial Agents; D001422:Bacterial Adhesion; D001672:Biocompatible Materials; D002516:Ceramics; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000166:cytology; D005782:Gels; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; C022107:Bioglass; D005782:Gels