title: Effects of selenium deficiency on the rat myocardial protein pattern-- investigation by two-dimensional gel electrophoresis.
abstract: Dietary selenium deficiency represents an etiological factor in "Keshan disease", a distinct form of an endemic cardiomyopathy. The biochemical effects of selenium depletion in the myocardium are, however, not yet known. Therefore, we investigated the changes in the myocardial protein pattern in rats after long-term selenium deficiency. The myocardial proteins were analyzed in samples from five selenium-depleted rats (Se-deficient group) and five rats supplied with adequate amounts of the element (Se-adequate group). Isoelectric focusing (IEF) with carrier ampholytes on large 2-DE gels was used for the separation of proteins in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for the second dimension. The protein patterns were evaluated by means of a computer-assisted gel analysis system. The biochemical identification of the proteins of interest was achieved by matrix-assisted laser desorption/ionization mass spectrometry (MALDI) or immunoblotting. On average, 588 +/- 68 protein spots were found on the gels. No significant difference in spot numbers existed between the groups. A pattern of 270 spots with identical positions was found on every gel; 247 of these spots were not saturated and used for quantitative comparison. Thirty-five, i.e., 14 %, differed significantly in their relative intensity in the two groups. Twenty-eight protein spots were decreased in the Se-deficient group and seven were increased. Sarcomeric creatine kinase M chain, alpha-myosin heavy chain (alpha-MHC) and myosin light chain 1 and 2 (MLC 1 and 2) were largely decreased in Se-deficiency. Three protein spots were increased by more than twofold or appeared only in the Se-deficient group. A mitochondrial creatine kinase was identified in this group. The results suggest that selenium deficiency affects myocardial energy metabolism and contractile proteins. These changes probably reflect non-specific alterations in heart failure.
authors.affiliation: Innere Medizin/Kardiologie, Humboldt Universität Charité, Deutsches Herzzentrum Berlin. Zagrosek@dhzb.de
authors.forename: V
authors.initials: V
authors.lastname: Regitz-Zagrosek
authors.forename: A
authors.initials: A
authors.lastname: Kyriakopoulos
authors.forename: K P
authors.initials: KP
authors.lastname: Pleissner
authors.forename: P
authors.initials: P
authors.lastname: Jungblut
authors.forename: E
authors.initials: E
authors.lastname: Fleck
authors.forename: D
authors.initials: D
authors.lastname: Behne
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D007091:Image Processing, Computer-Assisted; D009124:Muscle Proteins / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D009218:Myosins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012518:Sarcomeres / Q000378:metabolism; D012643:Selenium / Q000172:deficiency*
chemical_list: D009124:Muscle Proteins; D018994:Myosin Light Chains; D003402:Creatine Kinase; D018995:Myosin Heavy Chains; D009218:Myosins; D012643:Selenium