Unusual organization of the 5S ribosomal spacer in Dirofilaria repens: absence of a canonical spliced leader 1 sequence.
Comparing the 5S ribosomal RNA spacer of Dirofilaria repens and D. immitis, we found that in D. repens PCR generates two amplification products. In both products, the putative spliced leader 1 sequences (SL1) show some nucleotide substitutions compared to the canonical SL1 sequence of nematodes. SL I is a short sequence transferred to the 5' end of most messenger RNAs through a trans-splicing reaction. All the other filariae studied thus far amplify a single fragment showing a canonical SL1 sequence. D. repens appears thus peculiar.
Favia
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Istituto di Patologia Generale Veterinaria dell'Università degli Studi di Milano, Milan, Italy. favia@axrma.uniromal.it
Bazzocchi
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Cancrini
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Genchi
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Bandi
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D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D004182:Dirofilaria / Q000235:genetics*; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004275:DNA, Ribosomal; D018510:RNA, Helminth; D012341:RNA, Ribosomal, 5S