title: Synthesis and characterization of a sulfhydryl-reactive rhenium metal-ligand complex.
abstract: We describe the synthesis and spectral characterization of a rhenium metal-ligand complex. This complex reacts with sulfhydryl groups via an iodoacetamide side chain on the phenanthroline ligand and displays a high limiting anisotropy near 0.35 when excited at 442 nm. When covalently linked to human serum albumin, this complex displayed a mean decay time of about 1 micros. This decay time is appropriate for measuring rotational correlation times on the microsecond time scale as may occur for large macromolecular complexes.
authors.affiliation: University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Dattelbaum
authors.forename: O O
authors.initials: OO
authors.lastname: Abugo
authors.forename: J R
authors.initials: JR
authors.lastname: Lakowicz
mesh_terms: D005454:Fluorescence Polarization; D006801:Humans; D008024:Ligands; D012211:Rhenium / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D008024:Ligands; D013438:Sulfhydryl Compounds; D012211:Rhenium