[Validation of an ELISA for the measurement of human IgG against serogroup B Neisseria meningitidis protein].
An indirect ELISA test was optimised in which B meningoccocus outer membrane proteins included in the Cuban Vaccine known as VA-MENGOC-BC were used as capture antigents. Specific antibodies in serum samples from vaccinated individuals were detected using a human anti-IgG conjugate, i.e. alklayne phosphatase, thus the reaction developed with a specific substrate called p-nitrophenilphophate. Standard serum was obtained from a reference standard, the gage curve range was 625 to 20,000/mL. The control serum was selected from the most interesting areas for the samples, hence, assay characteristics were determined. Intra-assay, inter-assay and total inaccuracies were lower than 10% in the most linear area of the curve. Detectability was 700/mL. Recovery, paralelism and linearity studies showed an under 10% inaccuracy.
Ferriol Marchena
X
X
inmunoquimica@finlay.edu.cu
García Malberti
A M
AM
Ochoa Azze
R
R
Bravo Verde
I
I
Blanco González
R
R
Estrada González
E
E
Nerey Olivares
M
M
Martínez Rodríguez
J C
JC
D001426:Bacterial Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D009345:Neisseria meningitidis / Q000145:classification / Q000276:immunology*; D015203:Reproducibility of Results; D012703:Serotyping
D001426:Bacterial Proteins; D007074:Immunoglobulin G