Multi-functional biopolymer prepared by covalent attachment of galactomannan to egg-white proteins through naturally occurring Maillard reaction.
Protein-polysaccharide conjugate was prepared as a functional biopolymer using protein and polysaccharide via a Maillard-type reaction. Ovalbumin and lysozyme were conjugated with galactomannan under controlled heating and humidity conditions. The antioxidant effect of ovalbumin and the antimicrobial activity of lysozyme were enhanced by the glycosylation. The emulsifying properties of the egg protein were also significantly improved by the modification. The increase in lipid affinity due to the conjugation resulted in the enhancement of the radical scavenging ability of ovalbumin. The effectiveness of lysozyme and its glycosylated derivative in restricting the activity of a Gram-negative pathogen, Edwardsiella tarda in fish was also investigated.
Nakamura
S
S
Shimane University, Department of Living Science, Matsue, Japan. soichiro@edu.shimane-u.ac.jp
Kato
A
A
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry* / Q000633:toxicity; D002347:Carps; D003460:Crystallization; D020594:Edwardsiella / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D005393:Fish Diseases / Q000382:microbiology; D005690:Galactose / Q000031:analogs & derivatives; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D015416:Maillard Reaction; D008351:Mannans / Q000737:chemistry* / Q000633:toxicity; D009113:Muramidase / Q000494:pharmacology; D009153:Mutagens / Q000633:toxicity; D010047:Ovalbumin / Q000737:chemistry*; D051381:Rats
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001704:Biopolymers; D004655:Emulsions; D008351:Mannans; D009153:Mutagens; C012990:galactomannan; D010047:Ovalbumin; D009113:Muramidase; D005690:Galactose