[Standardization and evaluation of the modified vibriocidal assay].
The vibriocidal antibody test is a reliable and well-documented method to determine bacterial antibodies to Vibrio cholerae 01 antigens. It consists of mixing serum dilutions and a steady quantity of bacteria and supplement to cause cell lysis. Titer is determined by visual observation. In this paper, we implemented a change in the presented method where a pH and glucose indicator was added to the culture medium used to stop the reaction, which allowed a quicker reading by any person who are not very familiar with the carrying out of this test since the colour change in the plaque is quite evident.
Cedré Marrero
B
B
García Sánchez
H M
HM
García Imia
L G
LG
Talavera Coronel
A
A
D000818:Animals; D001431:Bacteriological Techniques; D001770:Blood Bactericidal Activity; D006801:Humans; D011817:Rabbits; D014734:Vibrio cholerae / Q000276:immunology*