A simple high-performance liquid chromatography method for the analysis of glucose, glycerol, and methanol in a bioprocess.
A reliable high-performance liquid chromatography-refractive index detection method for the simultaneous analysis of glucose, methanol, and glycerol in a bioprocess fermentation media using direct injection is presented. The validation studies show a satisfactory selectivity, linearity, accuracy, and recovery of the method. The lowest concentration detectable for glucose and methanol is 3.5 and 6.7 mg/100 mL, respectively. This method could be an attractive choice for the analysis of these compounds not only in fermentation media but also in biomedical and environmental samples.
Parpinello
G P
GP
Dipartimento di Biotecnologie Agrarie ed Ambientali, Università di Ancona, Italy.
Versari
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D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017077:Culture Media, Conditioned / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000032:analysis*; D005990:Glycerol / Q000032:analysis*; D000432:Methanol / Q000032:analysis*; D010843:Pichia / Q000254:growth & development / Q000378:metabolism*; D011786:Quality Control; D012680:Sensitivity and Specificity
D017077:Culture Media, Conditioned; D005947:Glucose; D005990:Glycerol; D000432:Methanol