Human glutathione-S-transferase: cloning and expression in Lactococcus lactis.
The glutathione-S-transferase A1 cDNA was amplified from the total RNAs of human liver by RT-PCR, and inserted into plasmid pMG36e. The hGSTA1 was expressed in Lactococcus lactis MG1363 and verified by SDS-PAGE and Western blot, purified by affinity chromatography and showed enzymatic activity.
Xiang
H
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
Wei
W
W
Zhang
Y
Y
Tan
H
H
D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D013294:Lactococcus lactis / Q000201:enzymology / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D018076:DNA, Complementary; D005982:Glutathione Transferase