Picomolar quantitation of free sulfite in foods by means of [57Co]hydroxocobalamin and radiometric chromatography of [57Co]sulfitocobalamin. Method, applications and significance of coexisting sulfides.
The concentration dependent reaction of sulfite with 57Co-labeled hydroxocobalamin (OH57CoCbl) to produce a sulfitocobalamin (SO(3)57CoCbl) adduct served as a quantification strategy for foodborne sulfite residues freely extracted into pH 5.2, 0.05 M acetate buffer. SO(3)57CoCbl was then resolved using SP-Sephadex C-25 gel chromatography and its radiometric detection allowed calculation of a standard logit plot from which unknown sulfite concentrations could be determined. The sulfite detection range was 6.0 nM-0.3 pM with respective relative standard deviations of 4.4-29.4% for 50-microl samples. Individual incidences of foodborne sulfite intolerances provoked by L-cysteine or sulfite additive use in bakery products, which remained undetected using conventional sulfite analytical methods, underscored the quantitative value of the method. The analytical significance and occurrences of detectable sulfides coexisting with foodborne sulfite residues was also addressed.
Beck
R A
RA
Framingham Institute for Food Science, Technology and Nutritional Biochemistry, Framingham State College, Framingham Center, MA 01701, USA. beckra@worldnet.att.net
Anes
J M
JM
Savini
L M
LM
Mateer
R A
RA
D002850:Chromatography, Gel / Q000379:methods*; D003037:Cobalt Radioisotopes; D005504:Food Analysis; D006879:Hydroxocobalamin / Q000737:chemistry*; D011874:Radiometry; D013440:Sulfides / Q000032:analysis*; D013447:Sulfites / Q000032:analysis*; D014805:Vitamin B 12 / Q000737:chemistry*
D003037:Cobalt Radioisotopes; D013440:Sulfides; D013447:Sulfites; C016763:sulfitocobalamin; D014805:Vitamin B 12; D006879:Hydroxocobalamin