Cloning of murine glycosyl phosphatidylinositol anchor attachment protein, GPAA1.
Glycosyl phosphatidylinositols (GPIs) are used to anchor many proteins to the cell surface membrane and are utilized in all eukaryotic cells. GPI anchoring units are attached to proteins via a transamidase reaction mediated by a GPI transamidase complex. We isolated one of the components of this complex, mGPAA1 (murine GPI anchor attachment), by the signal sequence trap method. mGPAA1 cDNA is about 2 kb in length and encodes a putative 621 amino acid protein. The mGPAA1 gene has 12 small exons and 11 small introns. mGPAA1 mRNA is ubiquitously expressed in mammalian cells, and in situ hybridization analysis revealed that it is abundant in the choroid plexus, skeletal muscle, osteoblasts of rib, and occipital bone in mouse embryos. Its expression levels and transamidation efficiency decreased with differentiation of embryonic stem cells. The 3T3 cell lines expressing antisense mGPAA1 failed to express GPI-anchored proteins on the cell surface membrane.
Hiroi
Y
Y
Department of Cardiovascular Medicine, University of Tokyo Graduate School of Medicine, Tokyo 113-8655, Japan.
Chen
R
R
Sawa
H
H
Hosoda
T
T
Kudoh
S
S
Kobayashi
Y
Y
Aburatani
H
H
Nagashima
K
K
Nagai
R
R
Yazaki
Y
Y
Medof
M E
ME
Komuro
I
I
D016475:3T3 Cells / Q000378:metabolism / Q000502:physiology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D016375:Antisense Elements (Genetics) / Q000378:metabolism; D001483:Base Sequence / Q000235:genetics; D018958:CD55 Antigens / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism; D016678:Genome; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism; D014018:Tissue Distribution
D016375:Antisense Elements (Genetics); D018958:CD55 Antigens; D018076:DNA, Complementary; C486253:Gpaa1 protein, mouse; D008562:Membrane Glycoproteins; D011257:Pregnancy Proteins; D011506:Proteins; D012333:RNA, Messenger; C101480:miniplacental alkaline phosphatase; D000469:Alkaline Phosphatase