Amplified fragment length polymorphism (AFLP) analysis of Listeria monocytogenes.
AFLP analysis using four selective primers was performed on a set of 33 Listeria monocytogenes including strains from patients and foods implicated in outbreaks, human sporadic cases or foods. Strains were tested belonging to serovars 1/2a, 1/2b, 1/2c, 3b, and 4b. Using one of the primers, the AFLP technique generated 20 different sized DNA fragments. The 33 cultures segregated into 14 different patterns, each comprising 7-12 different fragments. Although the method was not sufficiently discriminatory for epidemiological typing, AFLP analysis reconfirmed the observation that L. monocytogenes comprises two major genetic groups: group 1 includes strains of serovars 1/2a and 1/2c, while group 2 serovars 1/2b, 3b and 4b.
Ripabelli
G
G
Division of Gastrointestinal Infections, Public Health Laboratory Service Central Public Health Laboratory, London, UK.
McLauchin
J
J
Threlfall
E J
EJ
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D008088:Listeriosis / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length
D004269:DNA, Bacterial; D015247:Deoxyribonuclease HindIII