Differentiation of Candida strains by lectin-mediated agglutination kinetics.
The lectin-mediated agglutination kinetics of Candida albicans, Candida tropicalis, Candida glabrata, Candida krusei, Candida kefyr, and Candida parapsilosis strains isolated from immunocompromised patients was investigated. The rate of the lectin-induced cell agglutination depends on the physiological state of the yeast cell population. Therefore, the Candida strains have to be cultivated and investigated under identical conditions. Lentil lectin (prepared from Lens culinaris), castor lectin, and concanavalin A were used. Different yeast species showed different agglutination behaviour. Furthermore, the lectin-mediated rate of agglutination is a strain-specific property which makes it possible to distinguish between different yeast strains of the same species. It is concluded that the lectin-mediated agglutination kinetics allows reproducible differentiation of yeast strains of the same species.
Nenoff
P
P
Department of Dermatology, University of Leipzig, Germany. nenp@server3.medizin.uni-leipzig.de
Süss
K
K
Flemming
C
C
Haustein
U F
UF
D000371:Agglutination; D000372:Agglutination Tests; D002175:Candida / Q000737:chemistry / Q000145:classification*; D002177:Candidiasis / Q000276:immunology / Q000382:microbiology; D003208:Concanavalin A; D006801:Humans; D016867:Immunocompromised Host; D037102:Lectins; D037121:Plant Lectins; D013045:Species Specificity; D013997:Time Factors
D037102:Lectins; D037121:Plant Lectins; C033619:lentil lectin; D003208:Concanavalin A