Isolation and characterization of transposon-induced chlorate resistant mutants of the cyanobacterium Anabaena species PCC 7120.
Mutants of Anabaena sp. PCC 7120 resistant to chlorate were isolated using transposon mutagenesis. The Anabaena population of 5 x 10(7) cells ml(-1) and log phase Escherichia coli cultures in undisturbed conditions produced maximum exconjugants. Nitrate-promoted growth and cellular constituents observed in the parent were absent in the mutants. Nitrate repressed heterocyst formation and N2-fixation in the parent, but had little or no effect on the mutants.
Rai
A K
AK
Department of Botany, Banaras Hindu University, Varanasi, India.
Tiwari
S P
SP
D017033:Anabaena / Q000187:drug effects / Q000235:genetics*; D002704:Chlorates / Q000494:pharmacology*; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004351:Drug Resistance / Q000235:genetics; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics; D016254:Mutagenesis, Insertional; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000032:analysis
D002704:Chlorates; D004251:DNA Transposable Elements; D009591:Nitrogenase