title: Direct analysis of artemisinin in plasma and saliva using coupled-column high-performance liquid chromatography with a restricted-access material pre-column.
abstract: A previously established HPLC system with post-column derivatization for the analysis of artemisinin was coupled to an ADS (alkyl-diol silica) pre-column, allowing direct and repetitive injection of protein-rich fluids such as plasma. The limit of quantitation for 100 microl of plasma was 10 ng/ml (CV=10.5%) while concentrations down to 2 ng/ml could be quantified for 1.00 ml saliva samples (CV=11.1%). The system was linear in the tested range of 10-2000 ng/ml for plasma and 2-240 ng/ml for saliva samples, respectively. This paper introduces coupled column HPLC as a simplified method for the routine analysis of artemisinin in biological fluids.
authors.affiliation: Department of Pharmacy, Uppsala University, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Gordi
authors.forename: E
authors.initials: E
authors.lastname: Nielsen
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
authors.forename: D
authors.initials: D
authors.lastname: Westerlund
authors.forename: M
authors.initials: M
authors.lastname: Ashton
mesh_terms: D000962:Antimalarials / Q000032:analysis / Q000097:blood*; D037621:Artemisinins; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000962:Antimalarials; D037621:Artemisinins; D012717:Sesquiterpenes; C031327:artemisinin