Lymphatic absorption of fatty acids and cholesterol in the neonatal rat.
High-fat diets are essential in suckling animals to ensure adequate calories for postnatal growth, but their lymphatic transport of dietary lipids has not been characterized. We established a lymph fistula model in suckling rats to quantify intestinal uptake and lymphatic transport of dietary lipids and analyzed lipoprotein fractions. Suckling 19-day-old Sprague-Dawley rats had their mesenteric lymph ducts cannulated and gastroduodenal tubes inserted. After overnight recovery, [(3)H]triolein and [(14)C]cholesterol were infused for 6 h. Of the total dose, only 38% of triolein and 24% of cholesterol were transported in the lymph of suckling rats. Analyses of residual luminal contents and intestinal mucosal homogenate showed neither reduced absorption nor delayed mucosal processing of ingested lipids to be the cause. Thin-layer chromatographic analysis of radioactive mucosal lipids, however, showed a predominance of free fatty acids (60%) and free cholesterol (67%), implying impaired esterification capacity in these animals. We speculate that this reduced esterification allows for portal transport or direct enterocyte metabolism of dietary lipids.
Division of Pediatric Gastroenterology and Nutrition, Children's Hospital Medical Center, Cincinnati, OH 45267, USA.
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D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling / Q000378:metabolism*; D001053:Apolipoproteins / Q000032:analysis / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D002784:Cholesterol / Q000493:pharmacokinetics*; D002855:Chromatography, Thin Layer; D002914:Chylomicrons / Q000378:metabolism; D004041:Dietary Fats / Q000493:pharmacokinetics; D005227:Fatty Acids / Q000493:pharmacokinetics*; D005260:Female; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000737:chemistry / Q000378:metabolism; D008196:Lymph / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014304:Triolein / Q000493:pharmacokinetics; D014316:Tritium / Q000493:pharmacokinetics
D001053:Apolipoproteins; D002250:Carbon Radioisotopes; D002914:Chylomicrons; D004041:Dietary Fats; D005227:Fatty Acids; D014316:Tritium; D014304:Triolein; D002784:Cholesterol