Effect of the natural antioxidant 2"-O-glycosylisovitexin on superoxide and hydroxyl radical generation.
The inhibitory effect of 2"-O-glycosyl isovitexin (2"-O-GIV) isolated from green barley leaves on superoxide and hydroxyl radical generation was investigated using electron spin resonance spectroscopy. 2"-O-GIV exhibited dose-related inhibitory activity both towards superoxide generation and hydroxyl radical generation. 2"-O-GIV inhibited superoxide formation by 97% at a level of 25 mM and inhibited hydroxyl radical formation by 91% at a level of 500 microM. The results suggest that the inhibitory activity of 2"-O-GIV is due to trapping radicals.
First Department of Medicine, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602, Japan.
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Arimoto
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Ichinose
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Yoshikawa
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Shibamoto
D000975:Antioxidants / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005419:Flavonoids / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism*; D007529:Isoflavones; D013481:Superoxides / Q000378:metabolism*
C090563:2''-O-glycosylisovitexin; D000975:Antioxidants; D005419:Flavonoids; D007529:Isoflavones; D013481:Superoxides; D017665:Hydroxyl Radical