Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12.
Spontaneous mutations have been isolated in Escherichia coli that result in the constitutive expression of an emrKY promoter. These mutations were found to be single-nucleotide substitutions within the linker region of the sensor protein EvgS, which is part of a two-component regulatory system along with EvgA. In the linker mutants (evgSI and evgS4), emrKY expression became constitutive and MIC against sodium deoxycholate was 20 mg/ml, eight-fold higher than in the wild type. Furthermore, the start site of transcription from the promoter of emrKY was identified; EvgA was shown to bind at the -52 to -84 region by the footprinting experiment.
Department of Agricultural Chemistry, Kinki University, Nara, Japan.
A
A
Kato
H
H
Ohnishi
K
K
Yamamoto
E
E
Furuta
H
H
Tanabe
R
R
Utsumi
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C065601:BvgA protein, Bacteria; D017931:DNA Primers; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C489314:EvgA protein, E coli; D014157:Transcription Factors; C080662:bvgS protein, Bordetella pertussis