Steroid-inducible transcription of the 3beta/17beta-hydroxysteroid dehydrogenase gene (3beta/17beta-hsd) in Comamonas testosteroni.
The expression of the Comamonas testosteroni gene, encoding 3beta/17beta-hydroxysteroid dehydrogenase enzyme (3beta/17beta-HSD), was analyzed at the transcriptional level. Northern blot analysis detected a 1 kb transcript in bacterial cells grown in minimum media supplemented with Casamino acids and testosterone. Also this transcript was observed when cells were grown in presence of 1-dehydrotestosterone, androstenedione and 1,4-androstadien-3, 17dione, but not in presence of acetate, citrate, cholic acid, cholesterol, and cortisol. In addition, this effect was dependent on the presence of another carbon source in the growth medium used, revealing catabolite repression.
Developmental Genetics Section, Laboratory of Molecular Biology, National Institutes of Health, Bethesda, MD 20892, USA.
J E
JE
Cabrera
J L
JL
Pruneda Paz
S
S
Genti-Raimondi
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000728:Androgens / Q000494:pharmacology*; D015152:Blotting, Northern; D020604:Comamonas testosteroni / Q000201:enzymology / Q000235:genetics*; D003470:Culture Media; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006207:Half-Life; D007700:Kinetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D000728:Androgens; D003470:Culture Media; D012333:RNA, Messenger; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase