Cost effectiveness in the discrimination of leishmania species causing anthroponotic leishmaniases in Asia using selective enzymes.
In this study, an attempt was made to evaluate the usefulness of selective enzymes in the identification of Leishmania spp causing anthroponotic leishmaniasis in Asia, especially from a cost effectiveness point of view. For this purpose cellulose acetate electrophoresis was carried out to identify the Leishmania species of the Old World. After analyzing 11 enzymes 6PGDH was found to be the most polymorphic enzyme which could distinguish the WHO reference strains of the Leishmania species endemic in Asian countries like L.(L.) donovani (DD8), L. (L.) infantum (IPT-I), L.(L.) major (5ASKH), and L.(L.) tropica (K-27). Addition of another enzyme G6PDH improved the quality of diagnosis. Cost could be reduced manifold to discriminate the Asian Leishmania parasites by analyzing these two enzymes.
Shamsuzzaman
S M
SM
Department of Parasitology, Kochi Medical School, Nankoku City, Japan . shamsuzz@kochims.ac.jp
Hashiguchi
Y
Y
D000818:Animals; D001208:Asia; D003362:Cost-Benefit Analysis; D006801:Humans; D007124:Immunoenzyme Techniques / Q000191:economics* / Q000379:methods*; D007891:Leishmania / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000175:diagnosis / Q000469:parasitology*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000469:parasitology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000469:parasitology; D015800:Protozoan Proteins / Q000032:analysis
D015800:Protozoan Proteins