Plasmid pGS5 from the hyperthermophilic archaeon Archaeoglobus profundus is negatively supercoiled.
We present evidence that, in contrast to plasmids from other hyperthermophilic archaea, which are in the relaxed to positively supercoiled state, plasmid pGS5 (2.8 kb) from Archaeoglobus profundus is negatively supercoiled. This might be due to the presence of a gyrase introducing negative supercoils, since gyrase genes are present in the genome of its close relative A. fulgidus, and suggests that gyrase activity predominates over reverse gyrase whenever the two topoisomerases coexist in cells.
Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France. puri@umh.es
P
P
López-García
P
P
Forterre
J
J
van der Oost
G
G
Erauso
D019609:Archaeoglobus / Q000235:genetics* / Q000254:growth & development; D019641:DNA, Archaeal; D004278:DNA, Superhelical; D010957:Plasmids
D019641:DNA, Archaeal; D004278:DNA, Superhelical