[The clinical significance of multidrug resistance associated protein(MRP) gene expression in acute leukemia].
OBJECTIVE
To investigate the relationship between the expression of the multidrug resistance-associated protein(MRP) gene and clinical drug resistance in acute leukemia(AL).


METHODS
Semi-quantitative reverse transcriptase polymerase chain reaction was used to examine the expression of MRP gene in 65 bone marrow aspirates of AL patients and 15 normal volunteer's peripheral blood mononuclear cells. The expression level of MRP were expressed as ratio of MRP/beta 2M(beta 2-microglobulin) and the ratio of MRP/beta 2M > or = 0.3 was defined as MRP positive.


RESULTS
The level of MRP mRNA and the positive percentage of MRP in relapsed-refractory group were significantly higher than that in normal control group. In newly diagnosed group, the first complete remission rate of MRP negative patients(84%) was significantly higher than that of MRP positive(25%) patients. MRP expression level was different in leukemia sub-type. MRP and mdr-1 genes were examined simultaneously in 65 AL patients, the mean expression level and positive percentage of MRP and mdr-1 in clinical resistance group were significantly higher than those in non-resistance group. No significant correlation was found between the MRP and mdr-1 expression(rs = 0.1683).


CONCLUSIONS
High expression of MRP leads to drug resistance and it is an unfavorable factor to prognosis.
Second Affiliated Hospital, Hebei Medical University, Shijiazhuang.
F
F
Wang
Z
Z
Dong
J
J
Luo
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis*; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D018432:Drug Resistance, Multiple / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000096:biosynthesis*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009363:Neoplasm Proteins; D001613:beta 2-Microglobulin