Negative immunohistochemical detection of CD103 (alphaEbeta7 integrin) in the infiltrates of acute rejection in liver and kidney transplantation.
BACKGROUND
The infiltration of epithelium by CD8+ T lymphocytes in human renal or liver allografts is a critical feature of acute rejection. CD103 expression can be acquired in vitro by CD8+ cytotoxic T lymphocytes in response to allogeneic renal epithelial cells and promotes their adhesion to epithelium and subsequent lysis of epithelial cells. We investigated the expression of CD103 in T-cell infiltrates during acute renal or liver rejection (grade < III).


METHODS
Immunohistochemical detection of CD103 in 11 liver and 10 kidney transplant biopsies with histopathological diagnosis of acute rejection.


RESULTS
None of the infiltrates expressed detectable CD103, although positive controls were stained under our conditions.


CONCLUSIONS
Failure to detect CD103 in renal biopsies can be related to the early posttransplantation interval (<6 months) corresponding to a first rejection episode. In our hands, immunohistological detection of CD103 was not possible in the infiltrates of acute rejection in liver or kidney transplantation.
Service d'Anatomie et Cytologie Pathologiques, Faculté de Médecine, Lille, France.
E
E
Leteurtre
M C
MC
Copin
M
M
Labalette
C
C
Noel
D
D
Roumilhac
F R
FR
Pruvot
M
M
Lecomte-Houcke
B
B
Gosselin
J P
JP
Dessaint
D000208:Acute Disease; D015703:Antigens, CD / Q000032:analysis*; D017252:CD3 Complex / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D006084:Graft Rejection; D006801:Humans; D007150:Immunohistochemistry; D039001:Integrin alpha Chains; D016030:Kidney Transplantation / Q000009:adverse effects*; D016031:Liver Transplantation / Q000009:adverse effects*
D015703:Antigens, CD; D017252:CD3 Complex; D016827:CD8 Antigens; D039001:Integrin alpha Chains; C091367:alpha E integrins