Apoptosis induced by niacin-related compounds in K562 cells but not in normal human lymphocytes.
In our previous study, we found that niacin-related compounds induced apoptosis in human acute myelomonocytic leukemia cells, HL-60. We have investigated whether these compounds acted as inducers of apoptosis also in various other cell types. In human chronic myelogenous leukemia cells, K562, which are relatively resistant to various inducers of apoptosis, the apoptosis was induced by picolinic acid and dipicolinic acid in about 50% of the cells 5-10 mM via the caspase pathway, but was not at 1 mM. However, isonicotinamide did not induce apoptosis effectively in K562 cells. On the other hand, in normal human quiescent lymphocytes, the apoptosis was not induced by these compounds at the same concentrations. It is suggested that these compounds may induce apoptosis mainly in tumor cells. The change of intracellular peroxide levels was observed in the early phase of apoptosis induced by niacin-related compounds. We expect to make use of niacin-related compounds in the field of medicine.
Department of Life Science, Faculty of Bioresources, Mie University, Tsu, Japan.
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Ogata
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Takeuchi
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Fujita
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Shibata
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Okumura
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Taguchi
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D009525:Niacin / Q000031:analogs & derivatives* / Q000494:pharmacology; D009536:Niacinamide / Q000494:pharmacology; D010545:Peroxides / Q000378:metabolism; D010848:Picolinic Acids / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
D000970:Antineoplastic Agents; D010545:Peroxides; D010848:Picolinic Acids; D017382:Reactive Oxygen Species; D009536:Niacinamide; D009525:Niacin; C027681:isonicotinamide; C030614:picolinic acid; C004860:dipicolinic acid