The decapentaplegic morphogen gradient regulates the notal wingless expression through induction of pannier and u-shaped in Drosophila.
The morphogen gradient of Wingless, a Wnt family member protein, provides positional information to cells in Drosophila imaginal discs. Elucidating the mechanism that precisely restricts the expression domain of wingless is important in understanding the two-dimensional patterning by secreted proteins in imaginal discs. In the pouch region of the wing disc, wingless is induced at the dorsal/ventral compartment boundary by Notch signaling in a compartment-dependent manner. In the notum region of the wing disc, wingless is also expressed across the dorsal/ventral axis, however, regulation of notal wingless expression is not fully understood. Here, we show that notal wingless expression is established through the function of Pannier, U-shaped and Wingless signaling itself. Initial wingless induction is regulated by two transcription factors, Pannier and U-shaped. At a later stage, wingless expression expands ventrally from the pannier expression domain by a Wingless signaling-dependent mechanism. Interestingly, expression of pannier and u-shaped is regulated by Decapentaplegic signaling that provides the positional information along the anterior/posterior axis, in a concentration-dependent manner. This suggests that the Decapentaplegic morphogen gradient is utilized not only for anterior/posterior patterning but also contributes to dorsal/ventral patterning through the induction of pannier, u-shaped and wingless during Drosophila notum development.
Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology, Nishigonaka Myodaijicho, 444-8585, Okazaki, Japan.
Y
Y
Tomoyasu
N
N
Ueno
M
M
Nakamura
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D004843:Epistasis, Genetic; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009024:Morphogenesis / Q000235:genetics*; D009154:Mutation; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D051880:Receptors, Notch; D015398:Signal Transduction; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014921:Wings, Animal / Q000196:embryology; D051155:Wnt1 Protein
D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D011518:Proto-Oncogene Proteins; D051880:Receptors, Notch; D014157:Transcription Factors; D051155:Wnt1 Protein; C064196:dpp protein, Drosophila; C109259:pnr protein, Drosophila; C109258:ush protein, Drosophila; C074622:wg protein, Drosophila