Performance of female Rhipicephalus sanguineus (Acari: Ixodidae) fed on dogs exposed to multiple infestations or immunization with tick salivary gland or midgut tissues.
This investigation compared the effects of repeated infestations to immunization of dogs with tick salivary gland or midgut extracts on the feeding and fecundity performances of female Rhipicephalus sanguineus (Latrielle). In each immunized group, three tick-naive dogs were immunized three times with tick salivary gland or midgut extracts, and twice challenged at 21-d intervals by allowing 80 female and 40 male adult ticks to feed on each host. The repeated infestation group of three naive dogs was infested five times at 21-d intervals by the same numbers of ticks. The repeated infestation group showed a trend of reduced tick performance after the third infestation, but some of the tick performance parameters had recovered by the fifth infestation. Tick attachment was reduced by immunization with either tick salivary gland or midgut extract. Immunization with tick salivary gland extract had the greatest impact on the feeding period and engorgement weight of the female ticks. Immunization with tick midgut extract resulted in the greatest reduction of tick fecundity parameters, which included preoviposition, oviposition, and egg-incubation periods in addition to reduced egg production and egg viability. These results confirm that dogs can become resistant to R. sanguineus, and demonstrate that immunization with tick salivary gland or midgut extract has different effects on tick feeding and fecundity.
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210, USA.
S
S
Jittapalapong
R W
RW
Stich
J C
JC
Gordon
T E
TE
Wittum
O O
OO
Barriga
D000818:Animals; D004064:Digestive System / Q000276:immunology; D004283:Dog Diseases / Q000276:immunology / Q000469:parasitology* / Q000517:prevention & control; D004285:Dogs; D005247:Feeding Behavior; D005260:Female; D008297:Male; D012469:Salivary Glands / Q000276:immunology; D013983:Tick Control; D013984:Tick Infestations / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks / Q000502:physiology*; D014611:Vaccination