In vitro evaluation of teratogenic effects by time-varying MR gradient fields on fetal human fibroblasts.
The purpose of this study was to evaluate the influence on fetal cell growth in vitro of rapidly changing magnetic gradient fields such as those produced by the gradient coils of a typical magnetic resonance (MR) imager. The static magnetic field and the radiofrequency pulses were disabled during all measurements. Human fetal fibroblasts were placed within a specially designed MR-compatible incubation system inside the magnet. Trapezoid-shaped waveforms of 500 and 75 Hz base frequency and an amplitude of 2 mT were applied for 2-24 hours. Proliferation of the cells was monitored for 3 weeks after exposure. Cell cycle analysis was performed until 24 hours after exposure to detect alterations in cell division. Tests were performed under two different conditions of growth to detect increased as well as decreased proliferation effects. None of these tests showed differences in proliferation and cell cycle distribution between exposed and nonexposed cells.
Rodegerdts
E A
EA
Department of Radiology, Klinikum der Eberhard Karls Universität, Tübingen, Germany. enno.rodegerts@uni-tuebingen.de
Grönewäller
E F
EF
Kehlbach
R
R
Roth
P
P
Wiskirchen
J
J
Gebert
R
R
Claussen
C D
CD
Duda
S H
SH
D000016:Abnormalities, Radiation-Induced / Q000209:etiology*; D002453:Cell Cycle / Q000528:radiation effects*; D016923:Cell Death / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005069:Evaluation Studies as Topic; D005333:Fetus; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000196:embryology; D008279:Magnetic Resonance Imaging / Q000009:adverse effects*; D008875:Middle Aged; D011336:Probability; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric