[Efficacy of purine nucleotides on purinergic P2 platelet receptors by small angle light scattering].
The effect of purines on the activation and aggregation of thrombocytes in rats and rabbits was studied by the method of small-angle light scattering. The EC50 values of ADP, inducing the activation and aggregation of thrombocytes, reflect the sequence of the agonist action on various receptors: P2X1, 20-40 nM; P2Y1, 90-110 nM; P2YADP, 120-240 nM. It was demonstrated that ADP behaves as partial agonist not only with respect to P2X1 receptors, but with respect to P2Y1 receptors as well. Thrombocytes activated by 20 nM ADP or 100-nM ATP pass into a refracter state in the absence of further stimulation. The reaction halftime is tau 1/2 = 6.0 +/- 0.2 min for the cells activated with ADP and tau 1/2 = 16.5 +/- 0.2 min for ADP.
Sakaev
M R
MR
Department of Pharmacology, St. Petersburg Chemico-Pharmaceutical Academy, Russia.
Mindukshev
I V
IV
Lesiovskaia
E E
EE
Petrishchev
N N
NN
Krivchenko
A I
AI
D000244:Adenosine Diphosphate / Q000494:pharmacology / Q000502:physiology; D000255:Adenosine Triphosphate / Q000494:pharmacology / Q000502:physiology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D008027:Light; D008297:Male; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D011685:Purine Nucleotides / Q000494:pharmacology*; D058910:Purinergic P2 Receptor Agonists; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D058469:Receptors, Purinergic P2X; D058472:Receptors, Purinergic P2Y1; D012542:Scattering, Radiation
D011685:Purine Nucleotides; D058910:Purinergic P2 Receptor Agonists; D058469:Receptors, Purinergic P2X; D058472:Receptors, Purinergic P2Y1; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate