Cell-cell adhesion via the ECM: integrin genetics in fly and worm.
Integrins are essential for the development of the two genetically tractable invertebrate model organisms, the nematode worm Caenorhabditis elegans and the fruit fly Drosophila melanogaster. Just two integrins are present in C. elegans: one putative RGD binding integrin alphapat-2betapat-3, corresponding to Drosophila alphaPS2betaPS and vertebrate alpha5beta1, alphaVbeta1 and alpha8beta1, and one putative laminin binding integrin alphaina-1betapat-3, corresponding to Drosophila alphaPS1betaPS and vertebrate alpha3beta1, alpha6beta1 and alpha7beta1. In this review, the function of this minimal set of integrins during the development of these two invertebrates is compared. Despite the differences in bodyplan and developmental strategy, integrin adhesion to the extracellular matrix is required for similar processes: the formation of the link that translates muscle contraction into movement of the exoskeleton, cell migration, and morphogenetic interactions between epithelia. Other integrin functions, such as regulation of gene expression, have not yet been experimentally demonstrated in both organisms. Additional proteins have been characterised in each organism that are essential for integrin function, including extracellular matrix ligands and intracellular interacting proteins, but so far different proteins have been found in the two organisms. This in part represents the fact that the characterisation of the full set of interacting proteins is not complete in either system. However, in other cases different proteins appear to be used for similar functions in the two animals. The continued use of genetic approaches to identify proteins required for integrin function in these two model organisms should lead to the identification of the minimal set of conserved components that form integrin adhesive structures.
Wellcome/CRC Institute and Department of Anatomy, University of Cambridge, Tennis Court Rd, CB2 1QR, Cambridge, UK. nb117@mole.bio.cam.ac.uk
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Brown
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins; D002448:Cell Adhesion; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism; D005544:Forecasting; D006801:Humans; D039001:Integrin alpha Chains; D039641:Integrin beta Chains; D016023:Integrins / Q000145:classification / Q000235:genetics* / Q000502:physiology; D007448:Invertebrates / Q000235:genetics; D010641:Phenotype; D014714:Vertebrates / Q000235:genetics
D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D039001:Integrin alpha Chains; D039641:Integrin beta Chains; D016023:Integrins; C082188:if protein, Drosophila; C093515:pat-3 protein, C elegans