High resolution allelotype of microdissected primary nasopharyngeal carcinoma.
Nasopharyngeal carcinoma (NPC) is a common cancer in South China but is rare in other parts of the world. To better understand the molecular basis of this cancer, we performed high-resolution allelotyping on 27 microdissected primary tumors using 382 microsatellite markers. We have detected high frequencies of allelic imbalance on 3p (96.3%), 9p (85.2%), 9q (88.9%), 11q (74.1%), 12q (70.4%), 13q (55.6%), 14q (85.2%), and 16q (55.6%). Nonrandom allelic changes of 12q and 16q were revealed for the first time. In addition, loss of heterozygosity on chromosomal arms 1p (37.0%), 5q (44.4%), and 12p (44.4%) were also common in NPC. Multiple minimally deleted regions, 7-40 cM, were identified at 3p14-24.2, 11q21-23, 13q12-14, 13q31-32, 14q24-32, and 16q22-23. Frequent deletions of these minimally deleted regions implied the presence of tumor suppressor genes that may be involved in the development of NPC. Consistent loss of heterozygosity on 3p, 9p, and 14q in almost all tumors suggested that such changes are critical events in NPC tumorigenesis.
Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, SAR. kwlo@cuhk.edu.hk
K W
KW
Lo
P M
PM
Teo
A B
AB
Hui
K F
KF
To
Y S
YS
Tsang
S Y
SY
Chan
K F
KF
Mak
J C
JC
Lee
D P
DP
Huang
D000483:Alleles; D001706:Biopsy; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D002881:Chromosomes, Human, Pair 12; D002882:Chromosomes, Human, Pair 13; D002883:Chromosomes, Human, Pair 14; D002885:Chromosomes, Human, Pair 16; D002893:Chromosomes, Human, Pair 3; D002899:Chromosomes, Human, Pair 9; D004210:Dissection; D005819:Genetic Markers; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery
D005819:Genetic Markers