[Apoptosis of B lymphocytic leukemia induced by anticancer drugs and their cell cycle specificity].
OBJECTIVE
Evaluating the effect of apoptosis induced by anticancer drugs on human B lymphocytic leukemia cell lines (Nalm-6) and their cell cycle specificity.


METHODS
Nalm-6 cells were treated with various anticancer drugs for 8-24 hours. Apoptotic cells and their cell cycle specificity were measured by using hypodiploid DNA-FCM and TdT assay + DNA staining.


RESULTS
ADR, VP16, CPT, MTX, Ara-C could markedly induce apoptosis of Nalm-6 cells in S phase. CPM, PRD, 6MP were less capable of inducing apoptosis. CPM in high dose resulted in cell necrosis. PRD induced apoptosis in G1 phase, while 6MP induced apoptosis in G1 and S phase. The effect of CPM showed no marked cell cycle specificity.


CONCLUSIONS
Hypodiploid DNA-FCM and TdT assay + DNA staining can be used to detect both tumor cell apoptosis and their cell cycle specificity which is helpful to predict prognosis and to design new chemotherapy regimen.
Sun
X
X
Department of Medical Oncology, Sun Yet-Sen University of Medical Sciences, Guangzhou.
Saeki
K
K
Masahito
T
T
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006801:Humans; D007399:Interphase; D015448:Leukemia, B-Cell / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents