Discrimination of dimethylamphetamine and methamphetamine use: simultaneous determination of dimethylamphetamine-N-oxide and other metabolites in urine by high-performance liquid chromatography-electrospray ionization mass spectrometry.
A simple and sensitive method by high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been investigated for the simultaneous determination of dimethylamphetamine (DMA), its specific yet labile main metabolite dimethylamphetamine-N-oxide (DMAO), and other metabolites, methamphetamine (MA) and amphetamine (AP), in urine. A combination of Bond Elut SCX columns for the solid-phase extraction of urine and a semi-micro SCX column for LC separations provided satisfactory results. The use of acetonitrile/5mM ammonium acetate buffer adjusted to pH 4 (65:35, v/v) as the mobile phase at a flow rate of 0.2 mL/min was found to be the most effective. The detection limits were 5 ng/mL for DMAO, 10 ng/mL for DMA and MA, and 50 ng/mL for AP in the SIM mode.
Forensic Science Laboratory, Osaka Prefectural Police Headquarters, Japan.
M
M
Katagi
M
M
Tatsuno
A
A
Miki
M
M
Nishikawa
H
H
Tsuchihashi
D000697:Central Nervous System Stimulants / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D006801:Humans; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000652:urine*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods / Q000592:standards; D019966:Substance-Related Disorders / Q000175:diagnosis
D000697:Central Nervous System Stimulants; D008694:Methamphetamine; C003714:dimethylamphetamine