[The molecular-weight characteristics of the bacterial lectins and humus components in soil].
A method has been developed to determine the molecular-weight distribution of biologically active substances: bacterial lectins and soil humus compounds. The method based on the simultaneous centrifugation of samples and molecular weight standards in the density gradient of NaCl solutions or combined gradient of NaCl and CsCl solutions permits analysing biologically active substances: lectins, proteins, polysaccharides, protein-polysaccharide complexes, humus compounds in the interval of molecular weight of 13.7 kappa [symbol: see text] a to 2000 kappa [symbol: see text] a. The use of this method in the soil researches makes it possible to study the dynamics of change of molecular parameters of the soil organic matter depending on agrotechnical methods as well as to determine transformation regularities of microbial polysaccharides.
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
S K
SK
Votselko
G A
GA
Iutinskaia
E A
EA
Kovalenko
N S
NS
Kucheriavaia
D001412:Bacillus subtilis; D001426:Bacterial Proteins / Q000032:analysis*; D002499:Centrifugation, Density Gradient / Q000379:methods / Q000706:statistics & numerical data; D006812:Humic Substances / Q000032:analysis; D037102:Lectins / Q000032:analysis*; D008970:Molecular Weight; D011549:Pseudomonas; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D001426:Bacterial Proteins; D006812:Humic Substances; D037102:Lectins; D012987:Soil