[The effects of intraperitoneal injection of IL-2 recombinant adenovirus and/or IL-3 recombinant adenovirus on the hematological recovery of murine model receiving high dose chemotherapy].
OBJECTIVE
To investigate the effect of Ad-IL-2 and/or Ad-IL-3 on the hematological recovery of murine model receiving high dose chemotherapy.


METHODS
Ad-IL-2 and/or Ad-IL-3 were i.p. injected into experimental mice after high dose cyclophosphamide(200 mg/kg). RT-PCR analysis of peritoneal cells and cytokine levels in murine serum were detected to confirm gene transfering. The number of peripheral WBC, femur MNC and CFU-GM in bone marrow were observed continuously.


RESULTS
After i.p. injection of Ad-IL-2 and/or Ad-IL-3, the transfected IL-2 and IL-3 gene were identified by RT-PCR analysis of peritoneal cells, high level of IL-2 and/or IL-3 were detected in murine serum. After i.p. injection of AD-IL-3, the number of WBC, femur MNC and CFU-GM in bone marrow significantly increased, while i.p. injection of Ad-IL-2 had no effect on improving hematological recovery. And the effect of i.p. injection of both Ad-IL-2 and Ad-IL-3 was not significantly different from that of injection either of them.


CONCLUSIONS
i.p. injection of Ad-IL-3 can increase hematological recovery after high dose chemotherapy.
Department of Hematology, Changhai Hospital, Second Military Medical University.
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M
Yu
X
X
Cao
J
J
Yang
D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D003520:Cyclophosphamide / Q000009:adverse effects*; D015316:Genetic Therapy; D005822:Genetic Vectors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000187:drug effects*; D007376:Interleukin-2 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000628:therapy*; D007958:Leukocyte Count / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection
D007376:Interleukin-2; D007377:Interleukin-3; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003520:Cyclophosphamide