Detection of apoptosis in tissue sections.
During the last few years, detection of apoptosis has evolved from a predominantly morphological basis to the use of ever more specific techniques. The methods widely used to visualize DNA fragmentation in tissue sections are now supplemented by a variety of specific antisera against components of the cell death pathways. Essential requirements for apoptosis detection techniques include high sensitivity for apoptotic cells, the ability to differentiate between apoptotic and necrotic cell death and other forms of DNA damage, and the distinction between different stages of the cell death process. In this overview, we will focus on recent technical advances in apoptosis detection covering improvements of in situ DNA fragmentation techniques, as well as pointing out some of the new tools available for the detection of apoptotic cells in tissue.
Stadelmann
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Brain Research Institute, Department of Neuroimmunology, Vienna, Austria.
Lassmann
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D017304:Annexin A5 / Q000032:analysis; D000906:Antibodies / Q000276:immunology; D017209:Apoptosis; D053148:Caspase 3; D020169:Caspases / Q000276:immunology; D002467:Cell Nucleus / Q000648:ultrastructure; D004396:Coloring Agents; D003598:Cytoskeletal Proteins / Q000276:immunology; D004249:DNA Damage; D053938:DNA Fragmentation; D004256:DNA Polymerase I / Q000737:chemistry; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008954:Models, Biological; D009336:Necrosis
D017304:Annexin A5; D000906:Antibodies; D004396:Coloring Agents; D003598:Cytoskeletal Proteins; D004256:DNA Polymerase I; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases