Enhancement of human endometrial stromal decidualization by bestatin, an aminopeptidase-N inhibitor.
We have re-evaluated the effects of bestatin, an aminopeptidase-N (CD13) inhibitor, on in vitro decidualization of normal human endometrial stromal cells by using an in vitro decidualization activity assay. Bestatin did not show any effects on the viability of both the decidualizing stromal cells co-stimulated with 8-Br-cAMP and bestatin and the 8-Br-cAMP-induced decidualized stromal cells, or on prolactin release from the decidualized stromal cells. However, bestatin dose-dependently enhanced prolactin release from the decidualizing stromal cells co-stimulated with 8-Br-cAMP and bestatin. These results indicate that bestatin enhances cAMP-mediated decidualization of human endometrial stromal cells and suggests that membrane aminopeptidase-N in the human endometrium is involved in the regulation of endometrial differentiation by inhibiting cAMP-mediated decidualization signals in endometrial stromal cells.
Department of Obstetrics and Gynecology, Osaka City University Medical School, Japan.
T
T
Tanaka
N
N
Umesaki
M
M
Masuda
K
K
Mizuno
S
S
Ogita
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D018826:CD13 Antigens / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D003656:Decidua / Q000187:drug effects / Q000502:physiology*; D004717:Endometrium / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011388:Prolactin / Q000032:analysis / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology*
D004791:Enzyme Inhibitors; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011388:Prolactin; D018826:CD13 Antigens; D007930:Leucine; C012211:ubenimex