Analysis of programmed cell death in the nematode Caenorhabditis elegans.
The nematode Caenorhabditis elegans has been shown to be an excellent model organism with which to study the mechanisms of programmed cell death because of its powerful genetics and the ability to study cell death with single-cell resolution. In this chapter, we describe methods that are commonly used to examine various aspects of programmed cell death in C. elegans. These methods, in combination with genetic analyses, have helped identify and characterize many components of the C. elegans cell death pathway, illuminating the mechanisms by which these components affect programmed cell death.
Department of Molecular, Cellular, and Developmental Biology, University of Colorado at Boulder 80309-0437, USA.
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D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000235:genetics / Q000502:physiology; D029742:Caenorhabditis elegans Proteins; D020169:Caspases / Q000378:metabolism; D002467:Cell Nucleus / Q000648:ultrastructure; D002470:Cell Survival; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D018509:DNA, Helminth / Q000032:analysis / Q000378:metabolism; D012734:Disorders of Sex Development; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva; D008857:Microscopy, Interference / Q000379:methods; D010614:Pharynx / Q000166:cytology / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000032:analysis
D029742:Caenorhabditis elegans Proteins; D018509:DNA, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins; D020169:Caspases; D003546:Cysteine Endopeptidases; C089223:ced-3 protein, C elegans