Effects of unilateral deprivation in postnatal development of the olfactory bulb in an altricial rodent, the gerbil (Meriones unguiculatus).
To establish if olfactory bulb sensitivity to functional deprivation is related to the degree of development at birth, we studied the effects of surgical closure of one naris in the gerbil olfactory bulb development. The naris closure was performed at three different ages: at birth, P7 and P14 and maintained for 30 or 60 days. In coronal sections we measured total bulbar surface area and surface area of the different bulbar layers establishing an estimate multiple regression model for the percentage of surface area decrease in the deprived bulb related to non deprived one. The internal and external plexiform layers are the most sensitive layers to deprivation and age and duration of deprivation were factors in their mathematical models. The glomerular layer showed a surface reduction of about 25% without dependence either on age or duration. The deprived glomerular layer showed a much lower tyrosine hydroxylase-immunoreactivity and immunoreactive cell density than those in the non deprived one. However, differences in calbindin-immunoreactive and NADPH-diaphorase positive cell density between deprived and non deprived glomerular layer were not significant. Our results indicate that olfactory bulb sensitivity to functional deprivation is not related to the degree of precocity and changes in age and duration of deprivation cause different effects on the olfactory bulb layers.
Department of Otorhinolaryngology, Rio Hortega Hospital, University of Valladolid, Institute of Neuroscience of Castilla y León (INCYL), Av. Ramón y Cajal 7, 47005, Valladolid, Spain.
L A
LA
Vallejo
M
M
Garrosa
A
A
Al-Majdalawi
A
A
Mayo
M J
MJ
Gayoso
D000818:Animals; D000831:Animals, Newborn; D064026:Calbindins; D005260:Female; D007839:Functional Laterality / Q000502:physiology*; D005849:Gerbillinae / Q000502:physiology*; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis; D009473:Neuronal Plasticity / Q000502:physiology; D009666:Nose / Q000601:surgery; D009830:Olfactory Bulb / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000737:chemistry / Q000201:enzymology; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D012683:Sensory Deprivation / Q000502:physiology*; D012903:Smell / Q000502:physiology; D013045:Species Specificity; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D014446:Tyrosine 3-Monooxygenase; D009252:NADPH Dehydrogenase