Evaluation of the ADVIA Centaur TSH-3 assay.
An analytical evaluation of the thyroid stimulating hormone (TSH-3) assay on the Bayer ADVIA Centaur immunoassay system was performed. General analytical requirements (linearity, resistance to typical interferences, absence of a carry-over effect) were fulfilled and reproducibility was satisfactory. Inter-assay coefficient of variation (CV) of a human serum pool with a concentration of 0.014 mU/l was 22.3%; at concentrations between 0.26 and 83 mU/l CV was below 6%. Method comparison study demonstrated close agreement of TSH results compared to those obtained with the Roche Elecsys 2010 TSH assay (ADVIA Centaur = 1.08 x Elecsys - 0.18 mU/l; r=0.987; n=324). Handling and practicability of the ADVIA Centaur system proved to be convenient with a very high sample throughput. We conclude that the ADVIA Centaur TSH-3 assay meets requirements for clinical use.
Vogeser
M
M
Institute of Clinical Chemistry, Klinikum Grosshadern, Ludwig-Maximilians-Universität Munich, Germany. mvogeser@klch.med.uni-muenchen.de
Weigand
M
M
Fraunberger
P
P
Fischer
H
H
Cremer
P
P
D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015203:Reproducibility of Results; D013972:Thyrotropin / Q000097:blood*
D013972:Thyrotropin