Murine granulocytes control human tumor growth in SCID mice.
Severe combined immunodeficient (SCID) mice generally do not reject allogeneic or xenogeneic organ grafts and represent a unique model for investigating in vivo the behaviour of both normal and neoplastic human cells. However, cells from human primary tumors often do not grow in SCID mice. We have previously shown that the major reaction of SCID mice to the engraftment of human peripheral blood leukocytes is a massive granulocyte recruitment into the site of transplantation. In this study, we have investigated the role of murine granulocytes in the control of human tumor cell growth in SCID mice. We report here that murine granulocytes infiltrate and delimit the human tumor mass and that treatment of SCID mice with anti-murine granulocyte antibody markedly improves the growth of human tumor cell lines of different origin through suppression of the host granulocyte reaction. This finding provides a new tool for improving the human tumor take in SCID mice, thus opening new perspectives for a practical in vivo preclinical test of anti-tumor strategies. Moreover, this study, even with the limits of the known natural reaction against xenotransplants, further supports the importance of granulocytes in the control of tumor take and growth.
Lozupone
F
F
Laboratories of Immunology and Virology, Istituto Superiore di Sanità, Rome, Italy.
Luciani
F
F
Venditti
M
M
Rivoltini
L
L
Pupa
S
S
Parmiani
G
G
Belardelli
F
F
Fais
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D002455:Cell Division / Q000502:physiology; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology*; D020556:Neutrophil Infiltration / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal