Analysis of oligomannuronic acids and oligoguluronic acids by high-performance anion-exchange chromatography and electrospray ionization mass spectrometry.
Oligomannuronic acids and oligoguluronic acids were prepared by enzymatic hydrolysis of alginate with alginate lyases. The oligosaccharides generated up to degree of polymerization 16 were characterized by high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) and electrospray ionization mass spectrometry (ESI-MS). Acetate buffer linear gradients were used as mobile phases for separations of oligosaccharides. ESI-MS and HPAEC-PAD are very effective for the analysis and characterization of anionic oligosaccharides.
Food Science and Technology, Fishery College, Ocean University of Qingdao, China.
Y
Y
Liu
X L
XL
Jiang
H
H
Cui
H S
HS
Guan
D000837:Anion Exchange Resins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry; D006603:Hexuronic Acids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
D000837:Anion Exchange Resins; D006603:Hexuronic Acids; C007896:guluronic acid; C008324:mannuronic acid