The effect of a zinc, cobalt and selenium soluble glass bolus on trace element status and semen quality of ram lambs.
Supplemental zinc and selenium were administered to ram lambs grazed on pastures that were not considered to be deficient in either element. The breeding season and polygamy of the ram mean that his requirements for semen production will be relatively large over a short breeding season and this may induce a localised deficiency of zinc and/or selenium, thus resulting in a decrease in semen quality and production.Thirty-three 8-month-old ram lambs were kept at grass and fed a supplement of barley and peas, with ad libitum access to grass silage when grazing became restricted. On day 0, the rams were allocated to two groups by restricted randomisation of live weight. One group each had a zinc, cobalt and selenium soluble glass bolus (Zincosel(R), Telsol) administered with the other group not receiving a bolus to act as a control. Blood samples were taken by jugular venipuncture at day 0 (prior to bolus administration) and at days 23, 44, 65 and 86. Blood samples were analysed for zinc status (plasma zinc concentration) and selenium status (erythrocyte glutathione peroxidase activity). Semen was collected once a week between days 44 and 86, by diversion during a natural mount. Semen quality was assessed by ejaculate volume, spermatocrit, sperm concentration, abnormal morphology, motility, percentage live (negrosin-eosin stain), membrane integrity (hypo-osmotic swelling test (HOS)) and seminal fluid glutathione peroxidase activity and zinc concentration. The bolused lambs had a significantly increased erythrocyte glutathione peroxidase activity (P<0.01) on all samplings after bolusing and had significant increases in motility, proportion of live sperm and proportion of intact membranes indicated by the HOS. The bolused ram lambs had an increased selenium status and apparent improvement in semen membrane quality.
Centre for Animal Sciences, Leeds Institute of Biotechnology and Agriculture, School of Biology, University of Leeds, UK. nigel.kendall@nottingham.ac.uk
N R
NR
Kendall
S
S
McMullen
A
A
Green
R G
RG
Rodway
D000818:Animals; D003035:Cobalt / Q000008:administration & dosage / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000097:blood; D008297:Male; D012643:Selenium / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D012661:Semen / Q000187:drug effects / Q000502:physiology*; D012756:Sheep / Q000502:physiology*; D013054:Spectrophotometry, Atomic / Q000662:veterinary; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology*; D015032:Zinc / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*
D003035:Cobalt; D005979:Glutathione Peroxidase; D012643:Selenium; D015032:Zinc