Neisseria meningitidis porin PorB interacts with mitochondria and protects cells from apoptosis.
Neisserial porins are strong immune adjuvants and B cell activators. The effect of neisserial porin PorB on activation-induced cell death was investigated, as a potential additional mechanism of the porin's immunopotentiating ability. Neisserial porins interact with target cells to localize intracellularly in the mitochondrial compartment without negatively affecting cellular survival. Pretreatment with Neisseria meningitidis PorB porin decreased or abrogated the mitochondrial damage induced by apoptotic stimuli. In addition, end stage determinants of apoptosis, including DNA breakdown, were diminished by PorB. Immunoprecipitation experiments revealed that PorB interacts with the mitochondrial porin VDAC (voltage-dependent anion channel). The mechanism of the antiapoptotic effect of neisserial porins could be explained by the protein-protein interaction of PorB with VDAC, similar to the interaction of VDAC with antiapoptotic Bcl-2 proteins, resulting in an enhancement of cell survival and continued activation of B cells.
Massari
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Evans Biomedical Research Center, 650 Albany Street, Boston University School of Medicine, Boston Medical Center, Boston, MA 02118, USA.
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Wetzler
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D000818:Animals; D017209:Apoptosis; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D009345:Neisseria meningitidis / Q000378:metabolism*; D018272:Porins; D011485:Protein Binding
D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C057699:porin protein, Neisseria