[Regulative effect of nuclear factor kappa B on the expression of intercellular adhesion molecular-1 in human mesangial cells].
OBJECTIVE
To study the regulative effect of nuclear factor kappa B (NF-kappa B) on the expression of intercellular adhesion molecular-1(ICAM-1) in human mesangial cells (HMC) by interleukin I-1 beta (IL-1 beta).


METHODS
Activation of NF-kappa B was measured by electrophoresis mobility shift assay (EMSA). ICAM-1 expression was detected by Northern Blot and Cell ELISA.


RESULTS
rhIL-1 beta (10 ng/ml) could rapidly stimulate activation and translocation of NF-kappa B, and also could enhance the expression of ICAM-1 in mRNA (0.14 vs 0.35) and protein (0.92 +/- 0.10 vs 1.35 +/- 0.11, P < 0.01) level. After pretreatment with 100 mumol/L L-1-chlor-3-(4-tosylamido)-4-phenyl-2 butanon (TPCK), an inhibitor of NF-kappa B, both ICAM-1 levels of mRNA and protein stimulated by rhIL-1 beta were blocked by about 50% in these cells (0.46 +/- 0.05 vs 1.29 +/- 0.12, P < 0.01) compared with the rhIL-1 beta-stimulated group.


CONCLUSIONS
These results suggest that NF-kappa B is one of the signaling factors for IL-1-stimulated ICAM-1 expression in HMC. It may modulate the immune-inflammatory process in glomerular diseases.
Department of Nephrology, First Hospital, Beijing Medical University.
G
G
Guo
H
H
Wang
D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology*; D016328:NF-kappa B / Q000494:pharmacology*
D007375:Interleukin-1; D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1