Differential expression of D(1A) and D(1B) dopamine receptor mRNAs in the developing avian retina.
In the chick retina, the D1 dopaminergic system differentiates very early, as shown by receptor-mediated increases in intracellular cyclic AMP concentration and the presence of [(3)H]SCH23390-specific binding sites. Here, we characterized, by RT-PCR, the expression of defined D1 receptor subtypes D(1A), D(1B), and D(1D) during the development of the chick retina. Total RNA was extracted from retinas of 6-day-old embryos (E6) to 1-day-old hatched chickens and reverse-transcribed. The resulting cDNA was amplified using D(1A)-, D(1B)-, or D(1D)-specific primers, and the PCR-amplified products were analyzed by electrophoresis. The fragment corresponding to D(1A) receptor was detected in developing retina as early as E7, whereas the fragment corresponding to D(1B) was observed starting around E10. No PCR product corresponding to D(1D) was observed in the retina, although it was detected in chick brain. As synaptogenesis in chick retina begins after E11 and [(3)H]SCH 23390 D1 binding sites increase after this stage, the present results show that expression of D(1B) receptor increases during synaptogenesis, whereas D(1A) is the receptor subtype associated with the D1-like actions of dopamine early in retina development.
Soares
H C
HC
Departamento de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brazil.
de Melo Reis
R A
RA
De Mello
F G
FG
Ventura
A L
AL
Kurtenbach
E
E
D000818:Animals; D001552:Benzazepines / Q000493:pharmacokinetics; D001921:Brain / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens; D018507:Gene Expression Regulation, Developmental; D012333:RNA, Messenger / Q000235:genetics; D017447:Receptors, Dopamine D1 / Q000235:genetics* / Q000378:metabolism; D050641:Receptors, Dopamine D5; D012160:Retina / Q000196:embryology* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D001552:Benzazepines; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; C098593:dopamine D1A receptor; D050641:Receptors, Dopamine D5