[Segmented condensation of peptides on a solid phase using subtilisin complexed with sodium dodecyl sulfate].
The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the coupling of peptide segments on a solid phase in organic medium. By a two-stage enzymic condensation of peptide fragments on aminosilochrom (A) containing Met-Ala-Gly as a spacer, Dnp(or Boc)-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A and Z-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A were obtained. It was shown that the condensation products can be split off from the support using the Met residue cleavage by BrCN.
Kolobanova
S V
SV
Lomonosov State University, Chemical Department, Vorob'evy gory, Moscow, Russia.
Filippova
I Iu
IIu
Lysogorskaia
E N
EN
Getun
I V
IV
Bacheva
A V
AV
Stepanov
V M
VM
D010455:Peptides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry*
D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D013381:Subtilisins