Amylase hyper-producing haploid recombinant strains of Thermomyces lanuginosus obtained by intraspecific protoplast fusion.
Amylase hyper-producing, catabolite-repression-resistant, recombinant strains were produced by intraspecific protoplast fusion of thermophilic fungus Thermomyces lanuginosus strains, using well-characterized, morphological, and 2-deoxy-D-glucose resistant markers. The fusant heterokaryons exhibited enhanced amylase activities as compared to the amylase hyper-producing parental strain (T2). Diploids derived from heterokaryons segregated to stable haploid recombinant strains. In the haploid strain (Tlh 4q), approximately 5-fold higher specific activities of alpha-amylase and glucoamylase in the culture filtrate were observed as compared to the wild-type strain (W0).
Department of Microbiology, Guru Nanak Dev University, Amritsar, India.
K
K
Rubinder
B S
BS
Chadha
S
S
Singh
H S
HS
Saini
D000681:Amylases / Q000096:biosynthesis*; D002459:Cell Fusion; D003847:Deoxyglucose / Q000494:pharmacology; D004351:Drug Resistance; D006238:Haploidy; D006358:Hot Temperature; D003904:Mitosporic Fungi / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D011523:Protoplasts
D003847:Deoxyglucose; D000681:Amylases