[Effect of recombinant human growth hormone on hypoalbuminemia in peritoneal sepsis: experimental and clinical research].
OBJECTIVE
To investigate the effect of recombinant human growth hormone(rhGH) on hypoalbuminemia in peritoneal sepsis.


METHODS
We observed rhGH on albumin mRNA expression and albumin sythesis in vitro and in vivo with the method of reverse-transcriptional polymerase chain reaction (RT-PCR).


RESULTS
rhGH significantly up-regulated albumin mRNA expression in vitro (143 +/- 6 vs 100.4 +/- 2.2, P < 0.01) and in vivo(152 +/- 8 Vs 100 +/- 3, P < 0.01), and obviously alleviated the inhibition of albumin mRNA expression induced by LPS (P < 0.05). In cecal ligation and puncture-induced septic rats, rhGH significantly raised albumin synthesis(20.4 +/- 1.7 Vs 15.5 +/- 2.9 g/L, P < 0.01) and albumin mRNA expression. In patients with peritoneal sepsis, rhGH in combination with total parenteral nutrition markedly increased serum albumin, prealbumin, and transferrin concentration, but no apparent effect was observed in controls.


CONCLUSIONS
rhGH significantly increases albumin mRNA expression and albumin synthesis in peritoneal sepsis.
Li
W
W
Nanjing General Hospital of PLA.
Li
J
J
Gu
J
J
D000328:Adult; D000818:Animals; D005260:Female; D013006:Growth Hormone / Q000627:therapeutic use*; D006801:Humans; D007019:Hypoproteinemia / Q000209:etiology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010538:Peritonitis / Q000150:complications*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000627:therapeutic use; D012709:Serum Albumin / Q000172:deficiency*
D011994:Recombinant Proteins; D012709:Serum Albumin; D013006:Growth Hormone