Direct analysis of plasma samples for drug discovery compounds using mixed-function column liquid chromatography tandem mass spectrometry.
A sensitive, efficient, high throughput, direct injection bioanalytical method based on a single column and high-performance liquid chromatography (HPLC) with tandem mass spectrometry (MS/MS) was developed for pharmacokinetic analysis of early drug discovery compounds in plasma samples. After mixing with a working solution containing an internal standard each plasma sample was directly injected into a polymer-coated mixed-function column for sample cleanup, enrichment and chromatographic separation. The stationary phase incorporates hydrophilic polyoxyethylene groups and hydrophobic groups to the polymer-coated silica. This allows proteins and macromolecules to pass through the column due to restricted access to the surface of the packing while retaining the drug molecules on the bonded hydrophobic phase. The analytes retained in the column with a largely aqueous liquid mobile phase were then chemically separated by switching to a strong organic mobile phase. The column effluent was diverted from waste to the mass spectrometer for analyte detection. Within 200 plasma sample injections the response ratio (analyte vs. internal standard, %CV = 4.6) and the retention times for analyte and internal standard were found consistent and no column deterioration was observed. The recoveries of test compound in various plasma samples were greater than 90%. The total analysis time was </=5 min per sample.
Drug Metabolism and Pharmacokinetics Department, Schering Plough Research Institute, Kenilworth, NJ 07033, USA.
Y
Y
Hsieh
M S
MS
Bryant
G
G
Gruela
J M
JM
Brisson
W A
WA
Korfmacher
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D006801:Humans; D006900:Hydroxylation; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010599:Pharmacokinetics; D011092:Polyethylene Glycols; D011506:Proteins / Q000737:chemistry
D007202:Indicators and Reagents; D004364:Pharmaceutical Preparations; D011506:Proteins; D011092:Polyethylene Glycols