[Inhibition of apoptosis by bcr-abl fusion gene in K562 cells].
OBJECTIVE
To investigate the effect of bcr-abl fusion gene on CML cell apoptosis.


METHODS
Apoptosis of in vitro cultured K562 cells was observed after exposure to synthetic 18-mer antisense oligodeoxynucleotide complementary to the bcr-abl junction (b3a2).


RESULTS
Apoptosis of K562 cells was significantly increased associated with inhibition of bcr-abl expression.


CONCLUSIONS
bcr-abl fusion gene formation due to chromosome translocation may be the major mechanism of CML via inhibition of apoptosis.
Department of Medicine, 316 Hospital, PLA, Beijing.
C
C
Wang
B
B
Sun
Y
Y
Yuan
D017209:Apoptosis; D016044:Fusion Proteins, bcr-abl / Q000096:biosynthesis*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000378:metabolism / Q000473:pathology; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*
D020319:Oligodeoxyribonucleotides, Antisense; D016044:Fusion Proteins, bcr-abl