Inhibition of trypomastigotes' infectivity by a monoclonal antibody directed to a glycoconjugated fraction of epimastigotes of Trypanosoma cruzi.
In this work it was shown that the infectivity of trypomastigote forms of Trypanosoma cruzi was affected upon the interaction with the Monoclonal Antibody (McAb) 2E9, which was raised against a glycoconjugated fraction of membranes of epimastigotes (Tulahuen strain). Characterization of the epitope recognized by this McAb, as well as its effects on complement mediated lysis and host cell invasion are reported. Immunocytochemical analysis showed that the McAb was reactive with two macromolecules (41-58 kDa) present on Trypanosoma cruzi epimastigotes (Tulahuen and Y strain), while it recognized several trypomastigotes macromolecules, showing a more intense reactivity with a band of 80 kDa. By indirect immunofluorescence, it was found there were subpopulations of blood and tissue culture derived trypomastigotes which attach the antibody to varying degrees. Studies using chemical or enzymatically treated antigens suggested that the McAb 2E9 was directed against carbohydrate epitopes, which were identified as being--galactosyl residues. In addition, preliminary results are shown, suggesting that the epitope recognized by the McAb 2E9 is involved in adhesion/or internalization of trypomastigotes.
INTEBIO, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
M S
MS
Imaz
M
M
Esteva
E
E
Velázquez
A M
AM
Ruiz
E L
EL
Segura
A J
AJ
Marcipar
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D000913:Antibodies, Protozoan / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology; D002241:Carbohydrates / Q000276:immunology; D002460:Cell Line; D014355:Chagas Disease / Q000517:prevention & control; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect; D006001:Glycoconjugates / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D018512:Parasitemia / Q000517:prevention & control; D015800:Protozoan Proteins / Q000276:immunology; D013552:Swine; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D002241:Carbohydrates; D000939:Epitopes; D006001:Glycoconjugates; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins