[The use of monoclonal antibodies for detecting the common antigenic determinants of Brucella spp. and Yersinia enterocolitica O:9].
Monoclonal antibodies (McAb) 2AH10, specifically reacting with protein preparations having mol. wt. of 18 and 38 kD and not interacting with brucellar lipopolysaccharide (LPS) and protein-polysaccharide antigen, have been obtained. As shown with the use of McAb 2AH10, Brucella spp. and Yersinia enterocolitica O:9 possess common antigenic determinants, localized not only in the area of their LPS, which is generally known, but also in the area of their outer cell-wall proteins with mol. wt. 18 and 38 kD. The sensitivity of the solid-phase enzyme immunoassay and the latex agglutination test with the use of McAb 2AH10 is essentially higher in the detection of B. abortus and B. suis, than B. melitensis and B. rangiferi, as well as Y. enterocolitica O:9. Essential differences observed in the detected concentrations of different Brucella species and Y. enterocolitica O:9 are seemingly linked with different expression of specific antigenic determinants, detected with the use of McAb 2AH10 in the corpuscular antigens under study.
Research Institute for Plague Control of Siberia and the Far East, Irkutsk, Russia.
L M
LM
Mikhaĭlov
A M
AM
Titenko
M P
MP
Rudnik
O D
OD
Zakhlebnaia
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000942:Antigens, Bacterial / Q000032:analysis*; D002002:Brucella / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D005779:Immunodiffusion / Q000379:methods; D007841:Latex Fixation Tests / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012680:Sensitivity and Specificity; D015008:Yersinia enterocolitica / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes