Flagellin from an incompatible strain of Pseudomonas avenae induces a resistance response in cultured rice cells.
The host range of Pseudomonas avenae is wide among monocotyledonous plants, but individual strains can infect only one or a few host species. The resistance response of rice cells to pathogens has been previously shown to be induced by a rice-incompatible strain, N1141, but not by a rice-compatible strain, H8301. To clarify the molecular mechanism of the host specificity in P. avenae, a strain-specific antibody that was raised against N1141 cells and then absorbed with H8301 cells was prepared. When a cell extract of strain N1141 was separated by SDS-polyacrylamide gel electrophoresis and immunostained with the N1141 strain-specific antibody, only a flagellin protein was detected. Purified N1141 flagellin induced the hypersensitive cell death in cultured rice cells within 6 h of treatment, whereas the H8301 flagellin did not. The hypersensitive cell death could be blocked by pretreatment with anti-N1141 flagellin antibody. Furthermore, a flagellin-deficient N1141 strain lost not only the induction ability of hypersensitive cell death but also the expression ability of the EL2 gene, which is thought to be one of the defense-related genes. These results demonstrated that the resistance response in cultured rice cells is induced by the flagellin existing in the incompatible strain of P. avenae but not in the flagellin of the compatible strain.
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama Ikoma, Nara 630-0101, Japan. fsche@bs.aist-nara.ac.jp
F S
FS
Che
Y
Y
Nakajima
N
N
Tanaka
M
M
Iwano
T
T
Yoshida
S
S
Takayama
I
I
Kadota
A
A
Isogai
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005408:Flagellin / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D017343:Genes, Plant / Q000235:genetics; D007113:Immunity, Innate / Q000187:drug effects* / Q000235:genetics; D020287:In Situ Nick-End Labeling; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000166:cytology / Q000235:genetics / Q000382:microbiology*; D010935:Plant Diseases / Q000139:chemically induced / Q000382:microbiology*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D000906:Antibodies; D001426:Bacterial Proteins; D012333:RNA, Messenger; D005408:Flagellin