Estimation of stem cell fractions in peripheral blood stem cell harvest by using an SE-9000 hematology analyzer.
We inquired whether stem cell fractions in peripheral blood stem cell harvest could be precisely detected by using a hematopoietic progenitor cell (HPC) counting system applied to an automated hematology analyzer, SE-9000. Although there was an apparent increase in the HPCs 20 h after storage in nondiluted conditions, samples diluted with RPMI-1640 containing 1.0 mg/ml EDTA-2K showed a relatively stable number of HPCs. There was a significant relationship between HPCs and CD34 cells (n = 75, r = 0.769). This method may represent the least expensive and most time-effective way for stem cell estimation in harvest products.
Blood Transfusion Division, Kobe University Hospital, Kobe, Japan. saigo@med.kobe-u.ac.jp
K
K
Saigo
T
T
Sugimoto
S
S
Takeuchi
Y
Y
Kosaka
R
R
Ryo
S
S
Kumagai
D000925:Anticoagulants / Q000494:pharmacology; D018952:Antigens, CD34 / Q000032:analysis; D001331:Automation; D001772:Blood Cell Count / Q000295:instrumentation*; D001943:Breast Neoplasms / Q000097:blood; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D005260:Female; D019337:Hematologic Neoplasms / Q000097:blood; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007202:Indicators and Reagents; D007937:Leukapheresis; D010051:Ovarian Neoplasms / Q000097:blood; D013501:Surface-Active Agents / Q000494:pharmacology
D000925:Anticoagulants; D018952:Antigens, CD34; D002614:Chelating Agents; D007202:Indicators and Reagents; D013501:Surface-Active Agents; D004492:Edetic Acid