New insecticidal bufadienolide, bryophyllin C, from Kalanchoe pinnata.
Two insecticidal bufadienolides (1 and 2) were isolated from a methanol extract of the leaves of Kalanchoe pinnata by bioassay-guided fractionation. Compound 1 was identified as known bryophyllin A (bryotoxin C). The structure of new bufadienolide 2, named bryophyllin C, was determined by spectroscopic methods and the chemical transformation of 1. Compounds 1 and 2 showed strong insecticidal activity against third instar larvae of the silkworm (Bombyx mori), their LD50 values being evaluated as 3 and 5 microg/g of diet, respectively.
Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
U
U
Supratman
T
T
Fujita
K
K
Akiyama
H
H
Hayashi
D000818:Animals; D012831:Bombyx; D002018:Bufanolides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002770:Cholenes / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D007306:Insecticides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D007928:Lethal Dose 50; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*
D002018:Bufanolides; D002770:Cholenes; D007306:Insecticides; C416846:bryophyllin C; C087925:bufadienolide