Enhanced binding of aminoglycoside dimers to a "dimerized" A-site 16S rRNA construct.
In this work, we investigated the binding of a series of dimeric aminoglycoside molecules to (i) a 27 nt A-site 16S rRNA construct, and (ii) an artificially grafted 46 nt 'dimerized' A-site 16S rRNA construct. It was observed that the dissociation constants of dimeric aminoglycosides to the dimerized A-site 16S rRNA construct can achieve up to approximately 19-fold enhancement compared to the monomeric aminoglycoside molecules.
Department of Chemistry, Indiana University-Purdue University Fort Wayne, 46805, USA. tokj@ipfw.edu
J B
JB
Tok
G R
GR
Huffman
D000617:Aminoglycosides / Q000138:chemical synthesis / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry*; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D019281:Dimerization; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S