[A new trichromic safranin stain for the detection of Cryptosporidium parvum, Cyclospora cayetanensis, species of Microsporidia and Isospora belli in fecal material].
Cryptosporidium parvum, Isospora belli, Cyclospora cayetanensis and Microsporidia are frequent pathogens in the immunodeficient host, which may cause multiple infections. The above mentioned parasites are found in feces by the application of different specific tintorial techniques. The objective of this work was the development of a stain for the simultaneous detection of these parasites, reducing costs as well as the time taken to make the diagnosis. The safranin-trichrome stain is simple, chip and its results are similar to those of specific tints. All microorganisms are easy to detect and besides being perfectly distinguishable from fungi and faecal elements.
Facultad de Ciencias Bioquímicas y Farmacéuticas, Suipacha, Argentina.
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Ponce de León
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Flaherty
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Zdero
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000469:parasitology; D000818:Animals; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology; D004396:Coloring Agents; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D016783:Eucoccidiida / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D007549:Isospora / Q000302:isolation & purification*; D016816:Microsporida / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000175:diagnosis / Q000469:parasitology; D009282:Naphthalenesulfonates; D010270:Parasite Egg Count / Q000379:methods*; D010619:Phenazines; D010772:Phosphotungstic Acid; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; D009282:Naphthalenesulfonates; D010619:Phenazines; D012394:Rosaniline Dyes; D010772:Phosphotungstic Acid; C070620:chromotrope 2R; C035906:Fast Green; C009195:safranine T