Homomeric interaction of the mouse Rad52 protein.
The Rad52 protein plays a crucial role in repairing DNA damage and homologous recombination, possibly by virtue of its ability to catalyze annealing of single-stranded DNA. In agreement with recent genetic data, we here present results based on the two-hybrid system, demonstrating that mouse Rad52p is able to form homomeric complexes. A small domain necessary and sufficient for the self-interaction is located in the conserved N-terminus of the protein. These data contribute to the important insights into the architecture of the multi-protein complex involved in recombinational DNA repair.
Department of Animal Breeding and Genetics, Danish Institute of Agricultural Science, Research Centre Foulum, Tjele.
L
L
Krejci
B
B
Thomsen
M
M
Duno
O
O
Westergaard
C
C
Bendixen
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D017384:Sequence Deletion; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins