Rapid molecular typing of Listeria monocytogenes by pulsed-field gel electrophoresis.
Pulsed-field gel electrophoresis (PFGE) is a highly discriminating tool for molecular typing, but the conventional PFGE protocol is time consuming. This paper describes a rapid method of PFGE for Listeria monocytogenes that yields results within 2 days.
Department of Systematic Diagnosis, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. katsuda@niah.affrc.go.jp
K
K
Katsuda
M
M
Iguchi
T
T
Tuboi
K
K
Nishimori
K
K
Tanaka
I
I
Uchida
M
M
Eguchi
D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C038485:GGGCCC-specific type II deoxyribonucleases