Hormonal control on enzymes of osmoregulation in a teleost, Anabas testudineus (BLOCH): an in vivo and in vitro study.
Hormonal control of osmoregulation in teleosts is not well understood. Role of cGH, oGH, PRL, T3 and insulin on gill Na+,K+-ATPase, Mg2+ and Ca2+ ATPases was studied in A. testudineus. Short term administration of cGH, PRL or T3 significantly increased Na+,K+-ATPase, Mg2+ and Ca2+ ATPases, while oGH influenced only Mg2+ ATPase, and insulin stimulated Na+,K+-ATPase. Long-term treatment with cGH and PRL also significantly increased Na+,K+-ATPase activity. GH had an additive with T3 on stimulating Na+,K+-ATPase activity. In vitro addition of cGH and oGH also had definite stimulatory effect on gill Na+,K+-ATPase except for 2ng oGH. Bromocryptine treatment caused a significant reduction on Na+,K+-ATPase activity. Both in vivo and in vitro treatments of cGH and PRL independently reversed the action of bromocryptine on Na+,K+-ATPase. Combined treatment of cGH+PRL was more prominent in stimulating Na+,K+-ATPase in bromocryptine treated fish. Present study reveals that GH, PRL and T3 have definite regulatory role on enzymes of osmoregulation in the teleost Anabas testudineus.
Department of Zoology, University of Kerala, Trivandrum, India.
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D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000201:enzymology; D013006:Growth Hormone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D066298:In Vitro Techniques; D011388:Prolactin / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology; D014882:Water-Electrolyte Balance / Q000187:drug effects*
D006728:Hormones; D014284:Triiodothyronine; D001971:Bromocriptine; D011388:Prolactin; D013006:Growth Hormone; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase