Colocalization of numerous immunoreactivities in endocrine cells of the chicken proventriculus at hatching.
The colocalization of regulatory peptide immunoreactivities in endocrine cells of the chicken proventriculus at hatching has been investigated using the avidin-biotin technique in serial sections and double immunofluorescence in the same section for light microscopy, and double immunogold staining for electron microscopy. In addition to the eight immunoreactivities previously described in this organ, cells immunoreactive for peptide histidine isoleucine (PHI), peptide gene product 9.5 (PGP), and the amidating enzyme, peptidylglycine alpha-amidating monooxygenase (PAM) were observed. All the cells immunoreactive to glucagon were also immunostained by the PHI antiserum. In addition, all the glucagon-like peptide 1, avian pancreatic polypeptide, and some of the neurotensin-like cells costored also glucagon- and PHI-immunoreactive substances. PGP- and PAM-immunoreactivities were also found in the glucagon-positive cells. A small proportion of the somatostatin-containing cells were positive for PHI but not for other regulatory peptides. These results could suggest either the existence of a very complex regulatory system or that the endocrine system of the newborn chickens is not yet fully developed.
Department of Cytology and Histology, Universidad de Navarra, Pamplona, Spain.
A
A
Martínez
A M
AM
Buchan
J
J
López
P
P
Sesma
D000818:Animals; D001839:Bombesin / Q000276:immunology; D002645:Chickens; D005934:Glucagon / Q000276:immunology; D052216:Glucagon-Like Peptide 1; D006899:Mixed Function Oxygenases / Q000032:analysis* / Q000276:immunology; D009097:Multienzyme Complexes; D010191:Pancreatic Polypeptide / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D010451:Peptide PHI / Q000032:analysis* / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology; D011531:Proventriculus / Q000098:blood supply / Q000737:chemistry* / Q000294:innervation / Q000648:ultrastructure; D012701:Serotonin / Q000276:immunology; D013869:Thiolester Hydrolases / Q000032:analysis* / Q000276:immunology; D043222:Ubiquitin Thiolesterase
D009097:Multienzyme Complexes; D010446:Peptide Fragments; D010451:Peptide PHI; D011498:Protein Precursors; D012701:Serotonin; C009725:pancreatic polypeptide, avian; D010191:Pancreatic Polypeptide; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase; D001839:Bombesin