[Gene expression in vivo mediated by adenoviral vector after direct gene transfer into anastomotic rat carotid arteries].
OBJECTIVE
To observe the efficiency and time course of gene expression in vivo mediated by adenoviral vector after direct gene transfer into anastomotic rat carotid arteries.


METHODS
After being soaked in high concentration of glucose solution containing Adv5-CMV (control group) or Adv5-CMV/LacZ (treatment group) for 30 minutes, soluble stents were inserted into the lumina of cut rat carotid arteries and end-to-end anastomoses were performed. On the 2nd, 7th, 14th, 28th, 60th and 90th day after surgery, the anastomotic carotid arteries of the two groups were explanted. All samples were analyzed for the presence of beta-galactosidase activity and histochemical staining.


RESULTS
beta-galactosidase activity was not detected in the carotid arteries of the control group. Microscopic examination of sections from vessels of the control group revealed no X-gal staining. The treatment group revealed bluestaining in all anastomotic arteries and in all layers of the arterial wall explanted including the intima, media, and adventitia on the 7th, 14th day postoperatively.


CONCLUSIONS
Adenoviral vector could make foreign effectively transfer into rat carotid arteries in vivo. Mediated by adenoviral vector after direct gene transfer into anastomotic rat carotid arteries, the high level of gene expression only persisted about one month.
Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing.
Z
Z
Huang
J
J
Guo
S
S
Guo
D000260:Adenoviruses, Human / Q000235:genetics*; D000714:Anastomosis, Surgical; D000818:Animals; D002339:Carotid Arteries / Q000601:surgery; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015607:Stents; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase