[10-Hydroxycamptothecin induces apoptosis in human T24 urinary bladder cancer cells].
OBJECTIVE
To study the emechanisms of 10-Hydroxycamptothecine (HPT) inhibiting human urinary bladder cancer.


METHODS
Using flow cytometry, electron microscopy and fluorescence microscopy, we studied the effects of HPT on human T24 urinary bladder cancer cells.


RESULTS
HPT at 0.005 mg/L inhibited T24 bladder cancer cells by preventing the cells from S phase to G2/M phase and did not induce apoptosis. The apoptotic cells were induced by HPT at the concentration of 0.01-0.10 mg/L.


CONCLUSIONS
HPT can inhibit human T24 urinary bladder cancer cells and induced apoptosis at some concentrations. The induction of apoptosis is a very important mechanism of 10-Hydroxycamptothecine to treat urinary bladder cancer cells.
Fan
J
J
Department of Urology, Shanghai First People's Hospital.
Tang
X
X
Zhang
X
X
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D006801:Humans; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D000972:Antineoplastic Agents, Phytogenic; C028098:10-hydroxycamptothecin; D002166:Camptothecin