Obliterative lesions in a heterotopic bronchial xenograft model--a preliminary study.
BACKGROUND
We further developed our heterotopic pig model of obliterative bronchiolitis to study airway obliteration in xenografts.


METHODS
Four domestic piglets each received 40 bronchial xenografts s.c. from a donor lamb. Piglet X was not immunosuppressed. The other animals received daily oral cyclosporine, 15 mg/kg (XC), or SDZ RAD, 1.5 mg/kg (XR), or both (XCR). Five implants at a time were serially removed from each animal during 17 days for histological assessment.


RESULTS
In contrast to the grafts of the others, the xenografts of XCR recovered after initial ischemic damage. No epithelial damage (P<0.01) or mural necrosis occurred on day 7. Airway obliteration developed in all, but was significantly delayed in XCR.


CONCLUSIONS
Invariably developing airway obliteration in nontreated xenografts was delayed by immunosuppression, making the model useful, especially in testing the efficacy of immunosuppressive drugs in a xenogeneic system.
Salminen
U S
US
Department of Surgery, Helsinki University Central Hospital, Finland.
Maasilta
P K
PK
Taskinen
E I
EI
Harjula
A L
AL
D000818:Animals; D001980:Bronchi / Q000098:blood supply / Q000473:pathology / Q000637:transplantation*; D001989:Bronchiolitis Obliterans / Q000209:etiology*; D002356:Cartilage / Q000473:pathology; D016572:Cyclosporine / Q000494:pharmacology; D004848:Epithelium / Q000473:pathology; D000068338:Everolimus; D012756:Sheep; D020123:Sirolimus / Q000031:analogs & derivatives / Q000494:pharmacology; D012867:Skin; D013552:Swine; D014183:Transplantation, Heterologous; D016042:Transplantation, Heterotopic
D016572:Cyclosporine; D000068338:Everolimus; D020123:Sirolimus