Identification of mimotopes of platelet autoantigens associated with autoimmune thrombocytopenic purpura.
GPIIb/IIIa, the human platelet glycoprotein complex, is the autoantigen most commonly recognized by autoantibodies in autoimmune thrombocytopenic purpura (AITP). Two murine monoclonal antibodies (mAbs), namely Y2/51 and 5B12, directed against gpIIIa and gpIIb/IIIa, respectively, and rabbit anti-human platelet polyclonal antibodies have been used to select AITP-related epitopes from a phage display peptide library expressing random dodecapeptides in the pIII coat protein of M13 phage. The selected phage clones were tested by ELISA for binding to rabbit anti-human platelet antibodies as well as to sera from AITP patients. Seven clones reacted strongly with rabbit anti-human platelet antibodies, and four clones reacted with sera from AITP patients. Some homology between peptide inserts sequences of selected clones and human platelet gpIIIa and gpIb were found.
Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, D.F., Mexico. gokar@servidor.unam.mx
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G
Gevorkian
K
K
Manoutcharian
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T
Govezensky
J A
JA
Cano
V
V
Dominguez
H
H
Santamaria
C
C
Larralde
D000818:Animals; D001324:Autoantigens / Q000032:analysis*; D017104:Bacteriophage M13 / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D051379:Mice; D018716:Molecular Mimicry; D009842:Oligopeptides / Q000032:analysis / Q000276:immunology; D019151:Peptide Library; D010449:Peptide Mapping; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000276:immunology; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology*; D011817:Rabbits; D017386:Sequence Homology, Amino Acid
D001324:Autoantigens; D000939:Epitopes; D009842:Oligopeptides; D019151:Peptide Library; D019039:Platelet Glycoprotein GPIIb-IIIa Complex