Effect of high-fat diet, surrounding temperature, and enterostatin on uncoupling protein gene expression.
Nonshivering thermogenesis induced in brown adipose tissue (BAT) during high-fat feeding is mediated through uncoupling protein 1 (UCP1). UCP2 is a recently identified homologue found in many tissues. To determine the role of UCP1 and UCP2 in thermoregulation and energy balance, we investigated the long-term effect of high-fat feeding on mRNA levels in mice at two different ambient temperatures. We also treated mice with the anorectic peptide enterostatin and compared mRNA levels in BAT, white adipose tissue (WAT), stomach, and duodenum. Here, we report that high-fat feeding at 23 degrees C increased UCP1 and UCP2 levels in BAT four- and threefold, respectively, and increased UCP2 levels fourfold in WAT. However, at 29 degrees C, UCP1 decreased, whereas UCP2 remained unchanged in BAT and increased twofold in WAT. Enterostatin increased UCP1 and decreased UCP2 mRNA in BAT. In stomach and duodenum, high-fat feeding decreased UCP2 mRNA, whereas enterostatin increased it. Our results suggest that the regulation of uncoupling protein mRNA levels by high-fat feeding is dependent on ambient temperature and that enterostatin is able to modulate it.
Section for Molecular Signaling, Department of Cell and Molecular Biology, University of Lund, S-221 00 Lund, Sweden. catarina.rippe@medkem.lu.se
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Rippe
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Berger
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Böiers
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Ricquier
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Erlanson-Albertsson
D000273:Adipose Tissue / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D003089:Colipases / Q000378:metabolism / Q000494:pharmacology*; D004041:Dietary Fats / Q000378:metabolism* / Q000494:pharmacology; D004386:Duodenum / Q000378:metabolism; D004435:Eating / Q000187:drug effects; D004792:Enzyme Precursors; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D005951:Glucose Tolerance Test; D007473:Ion Channels; D008049:Lipase / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D026901:Membrane Transport Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D024101:Mitochondrial Proteins; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000494:pharmacology*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D013696:Temperature; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2
D001786:Blood Glucose; D002352:Carrier Proteins; D003089:Colipases; D004041:Dietary Fats; D004792:Enzyme Precursors; D007473:Ion Channels; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; C000604372:Ucp1 protein, mouse; C000604390:Ucp2 protein, mouse; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; C037107:procolipase; D008049:Lipase