Human monochromosome hybrid cell panel characterized by FISH in the JCRB/HSRRB.
The human monochromosome hybrid cell panel in the Japanese Collection of Research Bioresources (JCRB) consists of 23 mouse cell clones, each containing a different human chromosome (the Y chromosome is not yet included). The panel is currently distributed by the Human Science Research Resources Bank (HSRRB) in Osaka. In order to determine the state of the human chromosomes and to supply the information to investigators, we characterized the cells by fluorescence in-situ hybridization (FISH) with corresponding human chromosome-specific painting probes, and, in part, by reverse FISH with the hybrid total DNA hybridized onto human metaphase spreads. Here, we report the frequency of intact human chromosomes maintained in each hybrid and the retained subregions of corresponding human chromosomes with relative frequencies estimated by fluorescent intensity. We used specific painted patterns to classify each hybrid into tentative types with their frequencies showing the nature of each hybrid and the state of rearrangements. This characterization will provide valuable information to investigators using the panel.
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan. tanabe@nihs.go.jp
H
H
Tanabe
Y
Y
Nakagawa
D
D
Minegishi
K
K
Hashimoto
N
N
Tanaka
M
M
Oshimura
T
T
Sofuni
H
H
Mizusawa
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006224:Cricetinae; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008856:Microscopy, Fluorescence