Polarization transfer in lipid membranes.
Polarization transfer is a key experiment for the detection of insensitive nuclei by NMR. Transfer in liquids is often achieved through J-coupling using the INEPT experiment, while in solids the dipolar coupling is used with cross polarization. Liquid crystals, including lipid membranes, are intermediate cases between solids and liquids. In the present article, we compare several transfer methods for lipid membranes spinning at the magic angle. It is shown that the most commonly used cross polarization technique is, in most cases, advantageously replaced by refocused INEPT or even by the NOE enhancement experiment, a method that is not normally used in that context. In principle, these enhancement techniques could be applied to other systems, including biological tissues and, more generally, soft matter systems that are neither solid nor liquid by NMR standards.
Physico-Chimie Moléculaire des Membranes Biologiques, UPR 9052, Institut de Biologie Physico-Chimique, Paris, France.
D E
DE
Warschawski
P F
PF
Devaux
D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial
D008055:Lipids; D008567:Membranes, Artificial; D004134:Dimyristoylphosphatidylcholine