[Application of gas chromatography in the identification of Enterobacter cloacae, Enterobacter aerogenes, and Enterobacter agglomerans].
Enterobacter cloacae, Enterobacter aerogenes and Enterobacter agglomerans were identified using gas chromatography as a substitution of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 120 samples were analyzed from reference strains (ATCC 13047, 13048, 27155) and environmental isolations, eleven fatty acids were included in the profiles from which cis-9, 10-methyleneoctadecanoic acid (peak 24), cis-9-hexadecenoic acid (peak 14), octadecanoic acid (peak 23) and dodecanoic acid (peak 3), were the most important for the differentiation of the three species analyzed.
Unidad de Investigación Interdisciplinaria en Ciencias d e la Salud y la Educación, UNAM Campus Iztacala, México. erobles@servidor.unam.mx
E
E
Robles Valderrama
P
P
Ramírez García
M E
ME
González Arreaga
M G
MG
Sáinz Morales
B
B
Martínez Rodríguez
A
A
Durán Díaz
D
D
Chávez Ramírez
D001431:Bacteriological Techniques; D015415:Biomarkers; D002849:Chromatography, Gas; D004754:Enterobacter / Q000145:classification / Q000302:isolation & purification*; D016972:Enterobacter cloacae / Q000302:isolation & purification; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D019308:Palmitic Acid / Q000032:analysis; D013045:Species Specificity; D013229:Stearic Acids / Q000032:analysis
D015415:Biomarkers; D004952:Esters; D005227:Fatty Acids; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid; C029355:dihydrosterculic acid