[Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection].
OBJECTIVE
To demonstrate the relationship between H-ras oncogene and HCC metastasis.


METHODS
Activated H-ras oncogenes were transfected into SMMC7721, a cell line derived from human hepatocellular carcinoma (HCC), by calcium phosphate transfection method. Some metastasis-related parameters were detected in vitro, including adhesion assay, migration assay, expresion of collagenase IV (cIVase) and EGFR.


RESULTS
The abilities of H-ras-transfected cell clones in adhesion to laminin or fibronectin, migration, cIVase secretion increased markedly, and the expression of EGFR elerated moderately. More importantly, these alterations were consistently positive with the expression of p21, the protein product of H-ras oncogene.


CONCLUSIONS
H-ras oncogene could induce the metastatic phenotype of HCC cell in vitro, therefore enhancing its metastatic potential.
Liver Cancer Institute Shanghai Medical University.
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Wang
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Feng
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Lin
D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002448:Cell Adhesion; D002464:Cell Migration Inhibition; D066246:ErbB Receptors / Q000096:biosynthesis; D011905:Genes, ras; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000556:secondary; D009362:Neoplasm Metastasis; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
D066246:ErbB Receptors; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)