Patch-clamp study of liver nuclear ionic channels reconstituted into giant proteoliposomes.
Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope (NE) vesicles into planar lipid bilayer proceeded with low efficiency. NE vesicle reconstitution into proteoliposomes led to NIC observations by the patch-clamp technique. Large conductance, voltage-gated, K(+)-permeant and Cl(-)-permeant NICs were characterized. An 80-105-pS K(+)-permeant NIC with conducting sub-state was also recorded. Our data establish that NICs can be characterized upon reconstitution into giant proteoliposomes and retain biophysical properties consistent with those described for native NICs.
INSER U533, Hôtel-Dieu, Nantes, France. gilles.guihard@nantes.inserm.fr
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Guihard
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Proteau
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Payet
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Escande
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Rousseau
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002712:Chlorides / Q000378:metabolism; D004285:Dogs; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000378:metabolism / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism; D011510:Proteolipids; D051381:Rats
D002712:Chlorides; D007473:Ion Channels; D011510:Proteolipids; C020485:proteoliposomes; D011188:Potassium