Cloning and characterization of human histone deacetylase 8.
To date, seven different human histone deacetylases (HDACs) have been identified, which fall into two distinct classes. We have isolated and characterized a cDNA encoding a novel human HDAC, which we name HDAC8. HDAC8 shows a high degree of sequence similarity to HDAC1 and HDAC2 and thus belongs to the class I of HDACs. HDAC8 is expressed in a variety of tissues. Human cells overexpressing HDAC8 localize the protein in sub-nuclear compartments whereas HDAC1 shows an even nuclear distribution. In addition, the HDAC8 gene is localized on the X chromosome at position q13, which is close to the XIST gene and chromosomal breakpoints associated with preleukemia.
Department of Advanced Bio-Technologies, Jansen Research Foundation, Turnhoutseweg 30, 2340 Beerse, Belgium.
I
I
Van den Wyngaert
W
W
de Vries
A
A
Kremer
J
J
Neefs
P
P
Verhasselt
W H
WH
Luyten
S U
SU
Kass
D000595:Amino Acid Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D019457:Chromosome Breakage / Q000235:genetics; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006655:Histone Deacetylases / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D011230:Precancerous Conditions / Q000235:genetics; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D022661:RNA, Untranslated; D012097:Repressor Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection; D014960:X Chromosome / Q000235:genetics
D006657:Histones; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; D012097:Repressor Proteins; D014157:Transcription Factors; C077885:XIST non-coding RNA; C409470:HDAC8 protein, human; D006655:Histone Deacetylases