Interaction of wild-type and naturally occurring deleted variants of hepatitis B virus core polypeptides leads to formation of mosaic particles.
The simultaneous presence of hepatitis B virus (HBV) genomes carrying wild-type (wt) and in-frame deleted variants of the HBV core gene has been identified as a typical feature of HBV-infected renal transplant patients with severe liver disease. To investigate possible interactions of wt and deleted core polypeptides a two-vector Escherichia coli expression system ensuring their concomitant synthesis has been developed. Co-expression of wt and a mutant core lacking 17 amino acid residues (77-93) within the immunodominant region led to the formation of mosaic particles, whereas the mutant alone was incapable of self-assembly.
Institute of Virology, Humboldt University School of Medicine, Schumannstr, 20/21, D-10117, Berlin, Germany.
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Preikschat
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Kazaks
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Dishlers
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Pumpens
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Krüger
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Meisel
D015153:Blotting, Western; D019281:Dimerization; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D014644:Genetic Variation / Q000235:genetics*; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D006515:Hepatitis B virus / Q000737:chemistry / Q000235:genetics; D016056:Immunodominant Epitopes / Q000235:genetics; D008854:Microscopy, Electron; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D017384:Sequence Deletion / Q000235:genetics*; D014758:Viral Core Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure*; D019065:Virus Assembly
D006512:Hepatitis B Core Antigens; D016056:Immunodominant Epitopes; D010455:Peptides; D011994:Recombinant Proteins; D014758:Viral Core Proteins