Quantitative determination of the biodegradable polymer Poly(beta-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics.
Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(beta-hydroxybutyrate) (PHB) contents of bacterial cells. A total of 237 replicate spectra from 34 samples were obtained together with gas chromatography-determined reference PHB contents. Using the PLS regression, we were able to relate the infrared spectra to the reference PHB contents, and the correlation coefficient between the measured and predicted values for the optimal model with a standard error of prediction of 1.49% PHB was 0.988. With this technique, there are no solvent requirements, sample preparation is minimal and simple, and analysis time is greatly reduced; our results demonstrate the potential of FTIR spectroscopy as an alternative to the conventional methods used for analysis of PHB in bacterial cells.
Kansiz
M
M
Department of Chemistry, Monash University, Melbourne, Victoria, Australia.
Billman-Jacobe
H
H
McNaughton
D
D
D001673:Biodegradation, Environmental; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006885:Hydroxybutyrates / Q000032:analysis*; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D011091:Polyesters / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared; D000094122:Polyhydroxybutyrates
D004274:DNA, Recombinant; D006885:Hydroxybutyrates; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates