Theoretical description of the direct exponential amplification and sequencing (DEXAS) method.
We present a theoretical description of the method of DNA sequencing with simultaneous exponential PCR amplification of the template (DEXAS). Based on the theory of probability, the formula determining the optimal ratio of concentrations of deoxy- and dideoxynucleotides in the reaction mixture is derived, as well as the length distribution of sequenced DNA fragments. The prediction of the number of mutations is given and the theoretically determined aspects of DEXAS are compared with the corresponding quantities of classical sequencing methods. Some other experimentally observed effects are also discussed.
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic.
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M
Ingr
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Konvalinka
D004252:DNA Mutational Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA