cis-9,10-Methylenehexadecanoic acid inhibits contractility and actomyosin ATPase activity of guinea pig myocardium.
Superfusion with a cyclopropane fatty acid, cis-9, 10-methylenehexadecanoic acid (10-300 microM), reduced the contractility of papillary muscle isolated from guinea pigs in a dose-dependent manner. cis-9,10-Methylenehexadecanoic acid also inhibited the Mg(2+)-ATPase activity of guinea pig papillary myocardium by about 40% at 400 microM. Since cis-9, 10-methylenehexadecanoic acid 4 microM inhibited the K(+)-EDTA-ATPase activity inherent in myosin's catalytic activity by about 25%, the fatty acid was thought to interact with the catalytic center of the myosin molecule.
Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan. sakurada@m.u-tokyo.ac.jp
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D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D015151:Immunoblotting; D066298:In Vitro Techniques; D019787:Linoleic Acid / Q000494:pharmacology; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000201:enzymology*; D009218:Myosins / Q000037:antagonists & inhibitors*; D019308:Palmitic Acid / Q000494:pharmacology; D010169:Palmitic Acids / Q000494:pharmacology*; D010210:Papillary Muscles / Q000187:drug effects* / Q000201:enzymology; D010477:Perfusion; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010169:Palmitic Acids; D016718:Arachidonic Acid; D019308:Palmitic Acid; C008438:cis-9,10-methylenehexadecanoic acid; D019787:Linoleic Acid; D011493:Protein Kinase C; D009218:Myosins; D013755:Tetradecanoylphorbol Acetate