2-Bromoethylamine, a suicide inhibitor of tissue-bound semicarbazide-sensitive amine oxidase.
Various mammalian tissues contain plasma membrane-bound amine oxidase, termed semicarbazide-sensitive amine oxidase (SSAO). In the present study, 2-bromoethylamine has been studied with regard to inhibitory properties towards tissue-bound SSAO in rat lung. Without preincubation, 2-bromoethylamine was a competitive and reversible SSAO inhibitor with a Ki value of 2.5 microM. After preincubation, it time-dependently and non-competitively inhibited SSAO activity, probably by forming the covalently-bound enzyme-inhibitor adduct. The data presented suggest that 2-bromoethylamine may act as a suicide inhibitor of SSAO.
Laboratory of Enzyme Pharmacology, Senshu University at Ishinomaki, Japan.
H
H
Kinemuchi
M
M
Jinbo
A
A
Tabata
Y
Y
Toyoshima
Y
Y
Arai
T
T
Tadano
K
K
Kisara
L
L
Oreland
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors*; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005021:Ethylamines / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004791:Enzyme Inhibitors; D005021:Ethylamines; C004504:2-bromoethylamine; D006631:Amine Oxidase (Copper-Containing)