Elution mechanism in electrostatic ion chromatography with histidine as an isoelectric ampholytic mobile phase.
Electrostatic ion chromatography (EIC) using a zwitterionic stationary phase (formed by coating a C18 material with a hydrophobic zwitterionic surfactant) was studied with a mobile phase comprising an aqueous solution of histidine at the pH of its isoelectric point, together with non-suppressed conductometric detection. Anions and cations were found to be eluted as separate peaks, unlike the elution behaviour observed on the same system when pure water was used as mobile phase. An explanation was suggested in terms of protonation equilibria of the overall uncharged histidine to form small amounts of histidine cations and anions in the mobile phase which could act as counterions for analyte anions and cations. This suggestion was supported by measured pH changes occurring in the bands of eluted analyte anions (a decreased pH compared to the mobile phase) and cations (an increased pH compared to the mobile phase). The analytical potential of this type of EIC is discussed.
Macka
M
M
School of Chemistry, University of Tasmania, Hobart, Australia.
Haddad
P R
PR
D002853:Chromatography, Liquid / Q000379:methods*; D006639:Histidine / Q000737:chemistry*; D012680:Sensitivity and Specificity; D055672:Static Electricity
D006639:Histidine