Cloning of a new Bacillus thuringiensis cry1I-type crystal protein gene.
A new cry1I-type gene, cry1Id1, was cloned from a B. thuringiensis isolate, and its nucleotide sequence was determined. The deduced amino acid sequence of Cry1Id1 is 89.7%, 87.2%, and 83.4% identical to the Cry1Ia, Cry1Ib, and Cry1Ic proteins, respectively. The upstream sequence of the cry1Id1 structural gene was not functional as promoter in B. subtilis. The Cry1Id1 protein, purified from recombinant E. coli cells, had a toxicity comparable to that of Cry1Ia against Plutella xylostella, but it was significantly less active than Cry1Ia against Bombyx mori. Cry1Id1 was not active against the coleopteran insect, Agelastica coerulea.
Choi
S K
SK
Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience & Biotechnology, Yusong, Taejon.
Shin
B S
BS
Kong
E M
EM
Rho
H M
HM
Park
S H
SH
D000595:Amino Acid Sequence; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D012831:Bombyx; D003001:Cloning, Molecular; D001517:Coleoptera; D004731:Endotoxins / Q000235:genetics* / Q000302:isolation & purification; D006460:Hemolysin Proteins; D007306:Insecticides / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009036:Moths; D017434:Protein Structure, Tertiary
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis