Cationic liposome-enhanced adenoviral gene transfer in a murine head and neck cancer model.
The effect of combining adenoviral vector and cationic liposomes on the efficiency of gene transfer to head and neck tumor cells was investigated. Two human and two murine cell lines were used for the screening of gene transfer efficiency using an adenoviral vector. Cationic liposome-enhanced gene transfer was checked using a murine squamous carcinoma cell line, SCCVII/SF. A considerable difference in the efficiency of gene transduction was observed among the cell lines. The combination of DOSPER and adenoviral vector containing human alkaline phosphatase showed a remarkable enhancing effect in gene transfer in vitro and in vivo, compared to the adenovirus alone or control groups. With an improvement in the efficiency of gene transfer, it may be possible not only to enhance the expression of transduced genes, but also to deliver a smaller amount of virus, as a result, reducing toxicity and the immune response against adenovirus.
Sung
M W
MW
Department of Otolaryngology, Seoul National University College of Medicine, Korea. mwsung@snu.ac.kr
Lee
S G
SG
Yoon
S J
SJ
Lee
H J
HJ
Heo
D S
DS
Kim
K H
KH
Koh
T Y
TY
Choi
S H
SH
Park
S W
SW
Koo
J W
JW
Kwon
T Y
TY
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D008081:Liposomes; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
C118665:1,3-dioleoyloxy-2-(6-carboxyspermyl)propylamide; D005229:Fatty Acids, Monounsaturated; D008081:Liposomes