Specific types of prosomes distribute differentially between intermediate and actin filaments in epithelial, fibroblastic and muscle cells.
First observed as components of non-translated mRNP complexes, prosomes harbour RNase and several proteinase activities; they are also the central constituent of the "Multicatalytic Proteinase (MCP) complexes" or "26S-proteasomes". In two recent publications (Arcangeletti et al., 1997b; De Conto et al., 1997) we have shown, by applying a new fixation technique, that these particles distribute differentially between the cytoskeletal networks of intermediate filament (IF) and actin types; previously they had been observed exclusively on the intermediate filaments. Here we further investigate the distribution of prosomes of several types, distinct by their subunit composition, between the IF of vimentin type and the actin network, as well as in the 3D space of the cell. It is shown that subtypes of prosomes occupy specific networks of the cytoskeleton, and that this pattern is specific for a given cell type. Confocal microscopy shows that prosome cytodistribution is not homogeneous in the 3D space: in the perinuclear area they colocalize most strongly with the IF, and more peripherally with the microfilament/stress fiber system; connections may exist between the two networks. Furthermore, new data indicate that the prosome-actin interaction may participate in the molecular structure of the stress fibers.
Arcangeletti
C
C
Institut J. Monod, CNRS, Université Paris 7, France.
De Conto
F
F
Sütterlin
R
R
Pinardi
F
F
Missorini
S
S
Géraud
G
G
Aebi
U
U
Chezzi
C
C
Scherrer
K
K
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004847:Epithelial Cells / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D018613:Microscopy, Confocal; D009097:Multienzyme Complexes / Q000378:metabolism*; D009132:Muscles / Q000166:cytology*; D017830:Octoxynol / Q000494:pharmacology; D046988:Proteasome Endopeptidase Complex; D022502:Stress Fibers / Q000378:metabolism; D016707:Tissue Fixation / Q000379:methods
D000199:Actins; D000911:Antibodies, Monoclonal; D003902:Detergents; D009097:Multienzyme Complexes; D015638:Cytochalasin D; D017830:Octoxynol; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex