CD44v6 cell surface expression is a common feature of macrophages and macrophage-like cells - implication for a natural macrophage extravasation mechanism mimicked by tumor cells.
Soluble CD44standard (sCD44s) and CD44v6 (sCD44v6) cannot only be detected in sera of patients with pancreatic carcinoma but also of healthy blood donors. To investigate whether sCD44s and sCD44v6 are derived from white blood cells, we stimulated whole blood with phytohemagglutinin and interleukin-2, which induced expression of CD44v6 only on monocytes. For further investigations, we used the promyelocytic leukemia cell line Hl-60. Only Hl-60 cells differentiating along the macrophage pathway showed increased expression of CD44s and CD44v6. Furthermore, only macrophages showed increased secretion of sCD44s and sCD44v6. Our data suggest that CD44s and CD44v6 are common adhesion molecules on macrophages and macrophage-like cells.
Division of Molecular Oncology, University of Ulm, Germany.
H
H
Braumüller
S
S
Gansauge
M
M
Ramadani
F
F
Gansauge
D017398:Alternative Splicing; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology; D002762:Cholecalciferol / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D014644:Genetic Variation; D006023:Glycoproteins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D020033:Protein Isoforms / Q000097:blood / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins
C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D007376:Interleukin-2; D010835:Phytohemagglutinins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011994:Recombinant Proteins; D002762:Cholecalciferol; D007371:Interferon-gamma; D004121:Dimethyl Sulfoxide