Cellular uptake, subcellular localization and photodamaging effect of temoporfin (mTHPC) in nasopharyngeal carcinoma cells: comparison with hematoporphyrin derivative.
Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than hematoporphyrin derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.
Yow
C M
CM
Department of Nursing and Health Sciences, Hong Kong Polytechnic University, Hong Kong. hscyow@polyu.edu.hk
Chen
J Y
JY
Mak
N K
NK
Cheung
N H
NH
Leung
A W
AW
D000970:Antineoplastic Agents / Q000009:adverse effects* / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects; D005434:Flow Cytometry; D006415:Hematoporphyrins / Q000378:metabolism; D006801:Humans; D008297:Male; D008652:Mesoporphyrins / Q000009:adverse effects* / Q000378:metabolism*; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D010778:Photochemotherapy / Q000379:methods; D017319:Photosensitizing Agents / Q000009:adverse effects* / Q000378:metabolism*; D013053:Spectrophotometry; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D006415:Hematoporphyrins; D008652:Mesoporphyrins; D017319:Photosensitizing Agents; C072269:temoporfin