Molecular identification of Paracoccidioides brasiliensis by PCR amplification of ribosomal DNA.
We have amplified and sequenced the 5.8S and 28S ribosomal DNA genes and intergenic regions of Paracoccidioides brasiliensis, strain Pb01. Using primers specifically designed for both ribosomal DNA regions, we were able to discriminate between P. brasiliensis and other human pathogenic fungi by PCR. The use of this molecular marker could be important for paracoccidiodomycosis diagnosis and ecological and molecular epidemiological studies of P. brasiliensis in Latin America.
Laboratório de Biologia Molecular, Instituto de Biologia, Universidade de Brasília, 70910-900, Brasília, DF, Brazil.
A B
AB
Motoyama
E J
EJ
Venancio
G O
GO
Brandão
S
S
Petrofeza-Silva
I S
IS
Pereira
C M
CM
Soares
M S
MS
Felipe
D001483:Base Sequence; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics*; D010229:Paracoccidioidomycosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics
D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S