Estimation of fungal biomass and transcript levels in Tilia platyphyllos-Tuber borchii ectomycorrhizae.
Very little information is available to date about the complex truffle life cycle which involves the succession of three developmental phases. In order to gain more knowledge about ectomycorrhizal formation and fruit body development an ectomycorrhizal model system was used to study fungal biomass and plant and fungal transcript levels. They were evaluated in ectomycorrhizal development using the ergosterol assay and the internal transcribed spacer-5.8S ribosomal DNA from Tilia platyphyllos and Tuber borchii as molecular probes respectively. The results obtained from different approaches revealed a decrease in fungal biomass, transcript and protein levels during ectomycorrhizal development.
Zeppa
S
S
Istituto di Chimica 'Giorgio Fornaini' Università degli Studi di Urbino, 61029 Urbino, Italy.
Vallorani
L
L
Potenza
L
L
Bernardini
F
F
Pieretti
B
B
Guescini
M
M
Giomaro
G
G
Stocchi
V
V
D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development*; D018533:Biomass; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D004875:Ergosterol / Q000032:analysis; D005656:Fungal Proteins / Q000032:analysis; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010940:Plant Proteins / Q000032:analysis; D018517:Plant Roots / Q000235:genetics* / Q000382:microbiology*; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000032:analysis; D013559:Symbiosis
D017931:DNA Primers; D004271:DNA, Fungal; D018744:DNA, Plant; D005656:Fungal Proteins; D010940:Plant Proteins; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; D004875:Ergosterol