Divergence of glyceraldehyde-3-phosphate dehydrogenase isozymes in Saccharomyces cerevisiae complex.
Although sake yeasts are placed in Saccharomyces cerevisiae, we have been interested in their difference from the other subgroups of the species, and examined their proteins. When SDS-PAGE patterns of their soluble proteins were compared, specific differences between subgroups were found in their 36,000 Da regions. Proteins isolated therefrom were found to be subunits of three isomers of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from their N-terminal amino acid sequences and identified with anti-GAPDH serum. Therefore, comparison of zymogram was carried out by a modified method: denatured monomers were observed and the enzyme activity of their oligomers was not considered. SDS-PAGE patterns of all the sake yeasts differed from those of the other strains of S. cerevisiae. Strains of Saccharomyces bayanus showed uniform patterns which are different from the above two groups. Saccharomyces pastorianus strains resembled S. bayanus and were partly similar to S. cerevisiae in their patterns, in agreement with the hypothesis that S. pastorianus is a hybrid between these two species. Patterns of S. paradoxus appeared to be rather similar to those of sake yeasts. Results on the other species of the genus and on the preliminary experiments on PAGE of native isozymes are also described.
Kadokura
T
T
Department of Fermentation Science, Tokyo University of Agriculture, Setagayaku, Japan. kadokura@nodai.ac.jp
Ito
T
T
Takano
S
S
Nakazato
A
A
Hara
H
H
Watanabe
S
S
Kudo
T
T
Takeda
M
M
Kaneko
T
T
D000434:Alcoholic Beverages / Q000382:microbiology; D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry*; D007527:Isoenzymes / Q000737:chemistry; D008969:Molecular Sequence Data; D012440:Saccharomyces / Q000145:classification / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000201:enzymology*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D007527:Isoenzymes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases