IgM alloantibody detection by M-MPHA, a new solid-phase assay system.
New IgM alloantibody detection system that uses anti-human IgM-antibody-sensitized magnetic particles mixed passive hemagglutination (IgM-M-MPHA) has been developed. IgM antibodies are well detected by direct agglutination using the saline tube test. Anti-IgM using IgM antibody detection is not used routinely. In our evaluation, IgM-M-MPHA showed good detection of IgM alloantibodies. When IgM-M-MPHA is used together with IgG-M-MPHA (anti-human IgG-antibody-sensitized magnetic particles mixed passive hemagglutination), alloantibodies can be distinguished easily whether it is IgG, IgM or both.
Diagnostic Systems Division, Olympus Japan Co. Ltd., Ryumei-kan Building 4, Kanda-Surugadai 3, Chiyoda-ku, 101-0062, Tokyo, Japan. t-tamai@sales.olympus.co.jp
T
T
Toyohiro
I
I
Yasuyuki
M
M
Toshio
D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies