Altered caspase expression results in delayed neutrophil apoptosis in acute pancreatitis.
Acute pancreatitis (AP) may lead to the development of multiple organ dysfunction syndrome (MODS), especially in severe cases. Resolution of such inflammatory responses is dependent on neutrophil apoptosis. Delays in this apoptotic response are associated with persistent inflammation and subsequent tissue damage. The aim of this study is to determine the effects of AP on neutrophil apoptosis and to investigate the underlying mechanisms involved. Neutrophils and serum were isolated from control (n=10) and from patients with AP (mild, n=35, and severe, n=5). Neutrophil apoptosis was assessed by propidium iodide DNA staining using flow cytometry. Caspase, glutathione-S-transferase (GST), and Mcl-1 protein expression were assessed by SDS-PAGE western blotting. Serum interleukin (IL)-1beta and granulocyte-macrophage colony-stimulating factor (GM-CSF) levels were measured by ELISA. Neutrophils isolated from patients with AP show a significant delay in spontaneous neutrophil apoptosis. Serum factors contributed to this delay with increases in IL-1beta and GM-CSF. Isolated neutrophils were resistant to Fas antibody-induced apoptosis. Caspases represent a central mechanism for spontaneous and Fas antibody-induced neutrophil apoptosis. Procaspase 3 expression was decreased in mild and severe cases, but this effect was independent of serum factors. Increases in GST expression may also contribute to the antiapoptotic effect. Altered caspase expression may represent an additional factor contributing to delayed neutrophil apoptosis. This may contribute to the development of AP and its related complications.
Department of Surgery, Conway Institute of Biomolecular and Biomedical Research, University College Dublin and Mater Misericordiae Hospital, Ireland.
S
S
O'Neill
A J
AJ
O'Neill
E
E
Conroy
H R
HR
Brady
J M
JM
Fitzpatrick
R W
RW
Watson
D000208:Acute Disease; D000328:Adult; D000368:Aged; D017304:Annexin A5 / Q000097:blood; D017209:Apoptosis / Q000187:drug effects; D020169:Caspases / Q000096:biosynthesis / Q000097:blood / Q000235:genetics / Q000502:physiology*; D004789:Enzyme Activation; D004790:Enzyme Induction; D004792:Enzyme Precursors / Q000096:biosynthesis; D005260:Female; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000097:blood; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007375:Interleukin-1 / Q000097:blood; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008875:Middle Aged; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000097:blood; D009504:Neutrophils / Q000473:pathology*; D010195:Pancreatitis / Q000097:blood / Q000201:enzymology* / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2; D013997:Time Factors
D017304:Annexin A5; D004792:Enzyme Precursors; D007375:Interleukin-1; D008298:Maleates; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005982:Glutathione Transferase; D020169:Caspases; C014476:diethyl maleate