[Induction of apoptosis in human gastric carcinoma cell line MGC-803 by monoclonal antibody PD4].
OBJECTIVE
To examine if monoclonal antibody PD4 (McAb PD4) which identifies a surface antigen of 40 kD (P40) expressed in gastric carcinoma cells could induce tumor cell apoptosis.


METHODS
The antibody was tested for its effect on cell cycle and cell injury in gastric carcinoma cell MGC-803 by flow cytometry, DNA electrophoresis and TdT assay. Fas antigen expression in MGC-803 was also examined.


RESULTS
McAb PD4 could block tumor cell proliferation cycle and trigger cell death by inducing apoptosis, but expression of Fas antigen in MGC-803 cells was negative.


CONCLUSIONS
P40 is a tumor associated antigen which is distinct from Fas antigen. Molecular cloning of P40 will clarify the pathway and mechanism of apoptosis induced by this monoclonal antibody.
Beijing Institute for Cancer Research, School of Oncology, Beijing Medical University.
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H
Xiao
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Shou
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Dong
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D017209:Apoptosis; D006801:Humans; D007399:Interphase; D051379:Mice; D008807:Mice, Inbred BALB C; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000032:analysis
D000911:Antibodies, Monoclonal; D019014:fas Receptor