[Possibilities of demonstration of growth factors in chronic wounds. Demonstration of RNA and protein in wound tissue].
Growth factors are important modulators of wound healing. So far, there is little clinical data showing that healing defects in chronic wounds are attribute to deficiency of growth factor expression. Characterization of growth factor expression is necessary to provide new directions toward developing therapies in treating wounds. Present available molecular and immunological techniques allow the analysis of mRNA and protein expression in small amounts of wound material. We analyzed growth factor expression in wound tissue and wound fluid from acute and secondary healing wounds. Wound tissue of secondary healing wounds reveals a higher mRNA growth factor expression compared to acute wound tissue. Secondary healing wounds from our studies demonstrated no deficiency of growth factor expression however, functional analysis may be necessary to determine their activity.
Abteilung für Allgemeine Chirurgie (ZMF), Viktor von Bruns Laboratorien, Tübingen.
S
S
Wagner
E
E
Kimmerle
S
S
Coerper
H D
HD
Becker
D016228:Endothelial Growth Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006133:Growth Substances / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006652:Histological Techniques; D006801:Humans; D007150:Immunohistochemistry; D008222:Lymphokines / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism*
D016228:Endothelial Growth Factors; D006133:Growth Substances; D008222:Lymphokines; D020033:Protein Isoforms; D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors