Regulation of lymphocyte proliferation by eosinophils via chymotrypsin-like protease activity and adhesion molecule interaction.
We investigated the regulatory mechanisms responsible for release of eosinophil cationic protein (ECP) from eosinophils activated by platelet-activating factor (PAF) and monitored intra-cellular pH (pHi) changes using a pH-sensitive fluorescent probe. We also explored the mechanisms by which eosinophils suppress T-lymphocyte proliferation induced by phytohaemagglutinin (PHA). In these experiments, a separated culture to investigate the ECP-mediated pathway and a coculture to identify the adhesion molecules involved in eosinophil-lymphocyte interactions were employed. Chymostatin (1x10(-6) M) inhibited ECP release by about 50% via stimulation by PAF or recombinant interleukin 5(rIL-5) plus IgG. PAF (1x10(-7) M) raised eosinophil pHi from 6.9 to 7.3 within 20 s and pretreatment of these cells with chymostatin (1x10(-6) M), but not with leupeptin or E64-d, completely prevented this increase. Calcium ionophore A23187 (1x10(-7) M) induced ECP release and raised pHi to within a range similar to that of PAF, however, chymostatin had no effect on either. Chymostatin reversed ECP-mediated suppression of PHA-induced T-lymphocyte proliferation in separated cultures, but not in cocultures. In coculture, eosinophils exhibited the same level of suppression of both CD4(+) and CD8(+) T-cell proliferation in response to PHA. Monoclonal antibodies against CD11a, CD18 and CD54, but not CD11b, restored eosinophil suppression of T-lymphocyte proliferation which was chymostatin-resistant in coculture. Eosinophils were unable to suppress the proliferative response to lymphocytes to anti-CD3 stimulation. In conclusion, chymostatin specifically inhibited both the eosinophil pHi increase and ECP release induced by PAF. Eosinophils regulate PHA-induced T-lymphocyte proliferation via the ECP-mediation associated with chymotrypsin-like protease activity. These cells also control interactions with lymphocyte between adhesion molecules, CD11a, CD18 and CD54.
Matsunaga
Y
Y
Department of Internal Medicine and Health Care, School of Medicine, Fukuoka University, Fukuoka, Japan.
Shono
M
M
Takahashi
M
M
Tsuboi
Y
Y
Ogawa
K
K
Yamada
T
T
D001798:Blood Proteins / Q000378:metabolism; D017252:CD3 Complex / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism* / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D053818:Chymases; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000502:physiology; D011480:Protease Inhibitors / Q000494:pharmacology; D012260:Ribonucleases; D012697:Serine Endopeptidases / Q000378:metabolism*; D013347:Subcellular Fractions
D001798:Blood Proteins; D017252:CD3 Complex; D015815:Cell Adhesion Molecules; D047091:Eosinophil Granule Proteins; D005452:Fluoresceins; D010835:Phytohemagglutinins; D010972:Platelet Activating Factor; D011480:Protease Inhibitors; C057433:2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; D012260:Ribonucleases; D012697:Serine Endopeptidases; D053818:Chymases