Evaluation of LightCycler PCR for implementation of laboratory diagnosis of herpes simplex virus infections.
Five hundred specimens (288 genital, 192 dermal, and 20 ocular) were extracted by technologists, and the DNA was assayed by LightCycler PCR (DNA polymerase and thymidine kinase [TK] gene targets) and by conventional tube and shell vial cell culture. One hundred fifty-eight confirmed (by cell culture and TK target PCR) positive and LightCycler-positive specimens were detected during the first 30 PCR cycles. LightCycler PCR-positive results for cycles 31 to 45 (39 of 67 [58.2%]) required confirmation by another PCR target (TK). LightCycler PCR is more sensitive (n = 197; 23.1%) than cell cultures (n = 150) for the routine laboratory detection of herpes simplex virus infections.
Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
M J
MJ
Espy
T K
TK
Ross
R
R
Teo
K A
KA
Svien
A D
AD
Wold
J R
JR
Uhl
T F
TF
Smith
D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005069:Evaluation Studies as Topic; D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D018139:Simplexvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D013937:Thymidine Kinase / Q000235:genetics; D014776:Virus Cultivation
D013937:Thymidine Kinase; D004259:DNA-Directed DNA Polymerase