[Study on interphase cytogenetic abnormalities in malignant cells in pleural fluids from lung cancer cases].
OBJECTIVE
To study the interphase cytogenetic abnormalities in malignant cells in pleural fluids from lung cancer cases by fluorescence in situ hybridization(FISH) and compare the result of FISH with that of conventional cytology.


METHODS
Twenty-six pleural fluids from the lung cancer cases were detected by dual-fluorescence in situ hybridization centromere DNA probes of chromosomes 7, 11, 17, and X.


RESULTS
In 19 positive pleural fluids the rates of hyperdiploid in chromosomes 7, X, 17 and 11 were 16(84.2%), 14(73. 7%), 12(63.2%) and 8(42.1%), respectively. In 2 suspicious malignant pleural fluids, both chromosomes 7 and X showed hyperdiploid. Two cases of hyperplasia from 5 negative specimens also showed gain of chromosome X. Another 3 negative pleural fluids had a normal number of chromosome.


CONCLUSIONS
One of the important abnormalities occurred in tumor cells derived from pleural fluid of lung cancer is hyperdiploid, which can be detected by FISH. Therefore, hyperdiploid in pleural fluid cells can be an implication for the malignancy that has not been detected or confirmed by cytological examination.
Department of Cytology, Cancer Institute/Hospital, Chinese Academy of Medical Sciences, Beijing, P. R. China. jiadh@yeah.net
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Jia
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Zhang
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Liu
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Cheng
D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D016066:Pleural Effusion, Malignant / Q000473:pathology*; D011003:Ploidies