ADAMTS9, a novel member of the ADAM-TS/ metallospondin gene family.
ADAM-TS/metallospondin genes encode a new family of proteins with structural homology to the ADAM metalloprotease-disintegrin family. However, unlike other ADAMs, these proteins contain thrombospondin type 1 (TSP1) repeats at the carboxy-terminal end and are secreted proteins instead of being membrane bound. Members of the ADAM-TS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. We have cloned a new member of the ADAM-TS/metallospondin family designated here as ADAMTS9. This protein has a metalloprotease domain, a disintegrin-like domain, one internal TSP1 motif, and three carboxy-terminal TSP1-like submotifs. In contrast to other ADAM-TS family members, ADAMTS9 is expressed in all fetal tissues examined as well as some adult tissues. Using FISH and radiation hybrid analysis, we have localized ADAMTS9 to chromosome 3p14.2-p14.3, an area known to be lost in hereditary renal tumors.
Clark
M E
ME
Department of Molecular Biology, Neurocrine Biosciences Inc., San Diego, California 92121, USA. mclark@neurocrine.com
Kelner
G S
GS
Turbeville
L A
LA
Boyer
A
A
Arden
K C
KC
Maki
R A
RA
D051722:ADAM Proteins; D000071119:ADAMTS9 Protein; D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D019483:Disintegrins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019699:Thrombospondins / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D019483:Disintegrins; D019699:Thrombospondins; D051722:ADAM Proteins; D000071119:ADAMTS9 Protein; C412869:ADAMTS9 protein, human; D008666:Metalloendopeptidases