Neurotoxic effects of interleukin-6 and sodium nitroprusside on cultured rat hippocampal neurons.
The neurotoxicity of interleukin-6 (IL-6) and sodium nitroprusside (SNP, CAS 13755-38-9) was examined using primary cultures of rat hippocampal neurons. The cell viability was significantly reduced after the cultures were co-incubated with IL-6 4, 40, 400 ng/ml or SNP 1, 10, 100 mumol/l for 24 h. In addition, N omega-nitro-L-arginine (NNA, CAS 2149-70-4) at 0.1 mmol/l, when co-added with IL-6 400 ng/ml in cultures, significantly increased IL-6 reduced viability from 78.3 +/- 6.7% to 113.3 +/- 10.0%. These results indicate that IL-6 exerts neurotoxicity on cultured hippocampal neurons probably via overformation of nitric oxide in cultures.
Department of Pharmacology, Louisiana State University Medical Center, Shreveport, USA.
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D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D019335:Nitroarginine / Q000494:pharmacology; D009599:Nitroprusside / Q000633:toxicity*; D051381:Rats
D004791:Enzyme Inhibitors; D015850:Interleukin-6; D009599:Nitroprusside; D019335:Nitroarginine; C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat