Kinetics of cutinase catalyzed transesterification in AOT reversed micelles: modeling of a batch stirred tank reactor.
A transesterification process is analyzed in its multiple kinetic components that include the determination of the kinetic constants for both substrates, butyl acetate (BAc) and hexanol (H), involved in the alcoholysis reaction and for the products formed (hexyl acetate (HAc) and butanol (B)), participating into the reverse reaction. The order of magnitude of these constants is discussed in relation with the AOT/isooctane reverse micellar system under study. The values of the equilibrium conversion (X(e)) and constant (K(eq)) were also determined. Diffusional limitations were detected for H concentrations lower than 450 mM and the correspondent effectiveness factors were calculated. Above 450 mM H the reaction is kinetically controlled. The operation of a batch stirred tank reactor (BSTR) was modeled considering the integrated rate equation for reversible kinetics.
Carvalho
C M
CM
Laboratorio de Engenharia Bioquimica, Instituto Superior Técnico, Centro de Engenharia Biológica e Química, Av. Rovisco Pais, 1049-001, Lisbon, Portugal.
Aires-Barros
M R
MR
Cabral
J M
JM
D000085:Acetates / Q000737:chemistry / Q000378:metabolism; D019149:Bioreactors; D000440:Butanols / Q000737:chemistry / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D007700:Kinetics; D008823:Micelles; D008962:Models, Theoretical; D013386:Succinates / Q000737:chemistry / Q000378:metabolism*
D000085:Acetates; D000440:Butanols; D008823:Micelles; D013386:Succinates; D002265:Carboxylic Ester Hydrolases; C019952:cutinase; C024599:thiosuccinic acid