Induction of stress shock proteins DnaK and GroEL by phenoxyherbicide 2,4-D in Burkholderia sp. YK-2 isolated from rice field.
The purpose of this work was to investigate the induction of stress shock proteins in Burkholderia sp. YK-2 in response to the phenoxyherbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The stress shock proteins, which contribute to the resistance of the cytotoxic effect of 2,4-D, were induced at different 2,4-D concentrations in exponentially growing cultures of Burkholderia sp. YK-2. This response involved the induction of a 43-kDa DnaK and 41-kDa GroEL proteins, characterized by SDS-PAGE and Western blot by use of the anti-DnaK and anti-GroEL monoclonal antibodies. The total stress shock proteins were analyzed by 2-D PAGE. Survival of Burkholderia sp. YK-2 with time in the presence of different concentrations of 2,4-D was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain.
Cho
Y S
YS
Department of Life Science, Soonchunhyang University, Asan, Chung-Nam, South Korea.
Park
S H
SH
Kim
C K
CK
Oh
K H
KH
D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D001673:Biodegradation, Environmental; D015153:Blotting, Western; D019117:Burkholderia / Q000235:genetics / Q000378:metabolism*; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D006540:Herbicides / Q000378:metabolism / Q000494:pharmacology*; D012275:Oryza / Q000382:microbiology; D012988:Soil Microbiology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid; C063842:dnaK protein, E coli