The role of 5alpha-reductase activity in sexual behaviors of the green anole lizard.
Both testosterone (T) and its metabolite, 5alpha-dihydrotestosterone (DHT), can facilitate male sexual behavior in the lizard Anolis carolinensis. The present study addresses the role of DHT synthesis in regulating male sexual behavior by inhibiting 5alpha-reductase, the enzyme that converts T into DHT. In two separate experiments (one replacement and one maintenance paradigm), breeding adult males were castrated and implanted with capsules of T, DHT, or a control capsule (blank, BL). The animals were then injected with the 5alpha-reductase inhibitor, FCE, or with steroid suspending vehicle (SSV) as a control. Both experiments produced similar results. Overall, T was most effective in eliciting courtship and copulatory behaviors above control levels. In both experiments, treatment with FCE attenuated the T-induced effects on courtship behavior, whereas the inhibition of 5alpha-reductase activity resulted in modest and inconsistent effects on the latency to intromission and the proportion of copulating males. DHT treatment did not significantly increase courtship or copulatory behaviors above control levels. These results suggest that (a) 5alpha-reductase activity is necessary but that DHT alone is not sufficient for stimulating courtship in male A. carolinensis; and (b) courtship behavior is more sensitive than copulatory behavior to the activity of the androgen metabolizing enzyme.
Department of Psychology and Program in Neuroscience, Michigan State University, Psychology Research Building, East Lansing, MI 48824, USA.
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Rosen
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Wade
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D058891:5-alpha Reductase Inhibitors; D000704:Analysis of Variance; D000736:Androstenes / Q000008:administration & dosage; D000818:Animals; D001378:Azasteroids / Q000008:administration & dosage; D001921:Brain / Q000201:enzymology; D003380:Courtship; D013196:Dihydrotestosterone / Q000008:administration & dosage; D004343:Drug Implants; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005260:Female; D012739:Gonadal Steroid Hormones / Q000008:administration & dosage; D007279:Injections, Subcutaneous; D008116:Lizards / Q000378:metabolism*; D008297:Male; D011930:Reaction Time / Q000187:drug effects; D012726:Sexual Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D013739:Testosterone / Q000008:administration & dosage
C088574:(22RS-N-1,1,1-trifluoro-2-phenylprop-2-yl)-3-oxo-4-aza-5alpha-androst-1-ene-17beta-carboxamide; D058891:5-alpha Reductase Inhibitors; D000736:Androstenes; D001378:Azasteroids; D004343:Drug Implants; D004791:Enzyme Inhibitors; D012739:Gonadal Steroid Hormones; D013196:Dihydrotestosterone; D013739:Testosterone; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase