Feasibility of field-based light scattering spectroscopy.
Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 microns in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge 02139, USA. chyang@mit.edu
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Yang
L T
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Perelman
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Wax
R R
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Dasari
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Feld
D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D005240:Feasibility Studies; D005780:Gelatin / Q000737:chemistry / Q000648:ultrastructure; D007368:Interferometry; D008027:Light; D008863:Microspheres; D008962:Models, Theoretical; D019047:Phantoms, Imaging; D011137:Polystyrenes; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods* / Q000592:standards
D011137:Polystyrenes; D005780:Gelatin