Application of representational difference analysis to cloning a Mycoplasma arthritidis specific DNA fragment.
Representation difference analysis (RDA) was applied to isolate a Mycoplasma arthritidis specific DNA fragment. The DNA fragment obtained was verified to be M. arthritidis specific by polymerase chain reaction (PCR) and dot blot hybridization tests. The size of this fragment was 194 bp and the nucleotide sequence was also determined.
Kuo
C S
CS
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, ROC.
Hong
H S
HS
Chiou
J F
JF
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial