A simple and rapid method for isolating myelin basic protein.
The conduction of impulses along axons of nerves is facilitated by the myelin sheath, composed of proteins and lipid. Myelin basic proteins (MBPs) are extrinsic membrane proteins that play an important role in the structural organization of the myelin sheath. In the central nervous system, MBPs account for 30-40% of total protein. The traditional method of MBP isolation involves the use of chloroform-ethanol, which would destroy the native form of MBP. A modified method for maintaining its native form was developed. The white matter of porcine brain was directly extracted by buffers containing different concentrations of sodium chloride owing to MBP solubilized at high concentration of NaCl. The MBP was further purified by cation exchange chromatography and buffers containing glycine and salts. Purified MBP were consistently obtained by this method.
Department of Paediatrics, Faculty of Medicine, University of Hong Kong, China. richwong@hkucc.hku.hk
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D000818:Animals; D008722:Methods; D004676:Myelin Basic Protein / Q000302:isolation & purification*; D013552:Swine
D004676:Myelin Basic Protein