[Photodynamic effect of 49 single laser photosensitizers on two human tumor cell lines in vitro].
OBJECTIVE
For comparing the photodynamic effect of 49 single laser photosensitizers on two human cancer cell lines.


METHODS
After irradiation with a pulsed copper vapour laser, the SW1116 cell line of human colorectal cancer and K562 cell line of erythroleukemia were measured with MTT assay. IC50 and the cell survival rate were observed to evaluate the photodynamic effect of various 49 single photosensitizers.


RESULTS
In 49 photosentizers, the structure formula of the leading 9 single photosensitizers belonged to homatoporphyrin group, the average IC50 of which was < or = 1.0 microgram/ml on both cell lines of. The photosensitizers currently used in the clinic, hematoporphyrin-3 (PSD-007), ranked 17th, with IC50 on both cell lines of 1.25 micrograms/ml and 3.75 micrograms/ml, respecively.


CONCLUSIONS
The leading 16 single photosensitizer in ths series studied are better purified and more effective in photodynamic killing of human cancer cell lines in vitro. They can be used in lieu of PSD-007 for cancer treatment.
Cancer Institute, Zhejiang Medical University, Hangzhou.
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Zhang
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D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D006415:Hematoporphyrins; D017319:Photosensitizing Agents; D011838:Radiation-Sensitizing Agents; C052552:photocarcinorin