Diversity of N-acetylglucosamine-6-O-sulfotransferases: molecular cloning of a novel enzyme with different distribution and specificities.
N-Acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST) transfers sulfate to the C-6 position of non-reducing N-acetylglucosamine (GlcNAc) residues. We cloned human and mouse cDNAs encoding a novel GlcNAc6ST, designated as GlcNAc6ST-4, which showed sequence identities of 26 to 41% to other GlcNAc6STs. Human organs with strong expression of the enzyme mRNA were the heart, spleen, and ovary, while in the mouse strong expression was detected in the kidney. The enzyme expressed in CHO cells preferentially acted on mannose-linked GlcNAc, while a core 2 mucin-type oligosaccharide and an N-acetyllactosamine oligomer also served as acceptors. The distribution and the specificity of GlcNAc6ST are different from those of GlcNAc6STs identified previously.
Department of Biochemistry, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
K
K
Uchimura
F
F
Fasakhany
K
K
Kadomatsu
T
T
Matsukawa
T
T
Yamakawa
N
N
Kurosawa
T
T
Muramatsu
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D051379:Mice; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism; D009928:Organ Specificity; D010714:Phosphatidylethanolamines; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013431:Sulfates / Q000378:metabolism; D015238:Sulfotransferases / Q000096:biosynthesis / Q000235:genetics*; D000097811:Carbohydrate Sulfotransferases
D007527:Isoenzymes; D009844:Oligosaccharides; D010714:Phosphatidylethanolamines; D012333:RNA, Messenger; D013431:Sulfates; D015238:Sulfotransferases; D000097811:Carbohydrate Sulfotransferases; C100004:transfectACE; C412374:N-acetylglucosamine-6-O--sulfotransferase-4