Comparison of urinary desmosine excretion in patients with chronic obstructive pulmonary disease or cystic fibrosis.
Neutrophil elastase is involved in the pathogenesis of several pulmonary diseases; a strategy for monitoring in vivo elastase activity is to measure changes in biochemical markers. The objective of this study was to determine whether differences in the urinary excretion of the elastin crosslinks, desmosine and isodesmosine (which are unique amino acid products of elastase activity), could be discerned between groups of patients with chronic obstructive pulmonary disease (COPD) or cystic fibrosis (CF), and non-diseased, age-matched controls. Twenty-four-hour urine collections were analysed to eliminate variations in excretion throughout the day, and urine was collected on four separate days in 29-31 subjects/group to investigate the variability in desmosines excretion among the groups. Both sets of patient populations had significantly more variable desmosines readings (higher standard deviations) relative to their respective age-matched control group. The means for three adult groups (COPD, controls and a COPD-smoker subset) ranged from 28.4 to 35.5 pmol desmosines/mg creatinine and there were no differences among the groups. Values in children were higher: 55 pmol desmosines/mg creatinine in the non-CF children and 77 pmol desmosines/mg creatinine for the CF group (P<0.01 vs. age-matched controls). The results of this study show that urinary desmosines, as a surrogate marker for enhanced elastase activity, are more highly variant in both patient populations relative to age-matched controls, and an overall increase in the mean value is further observed in patients with cystic fibrosis.
Bode
D C
DC
Sterling Winthrop Pharmaceuticals Research Division, Collegeville, Pennsylvania, USA.
Pagani
E D
ED
Cumiskey
W R
WR
von Roemeling
R
R
Hamel
L
L
Silver
P J
PJ
D015415:Biomarkers / Q000652:urine; D016022:Case-Control Studies; D002648:Child; D003550:Cystic Fibrosis / Q000201:enzymology / Q000652:urine*; D003895:Desmosine / Q000652:urine*; D005260:Female; D006801:Humans; D007524:Isodesmosine / Q000652:urine*; D019272:Leukocyte Elastase / Q000378:metabolism*; D008173:Lung Diseases, Obstructive / Q000201:enzymology / Q000652:urine*; D008297:Male; D008875:Middle Aged
D015415:Biomarkers; D003895:Desmosine; D007524:Isodesmosine; D019272:Leukocyte Elastase