Enteric virus infections and diarrhea in healthy and human immunodeficiency virus-infected children.
Forty-three stool samples from 27 human immunodeficiency virus (HIV)-seropositive children and 38 samples from 38 HIV-negative children, collected during a 15-month period, were examined for enteric viruses. Diagnostic assays included enzyme immunoassays for rotavirus, adenovirus, and Norwalk virus; polyacrylamide gel electrophoresis for picobirnavirus and atypical rotavirus; and PCR for astrovirus and enterovirus. Specimens from HIV-positive children were more likely than those of HIV-negative children to have enterovirus (56 versus 21%; P < 0.0002) and astrovirus (12 versus 0%; P < 0.02), but not rotavirus (5 versus 8%; P > 0.5). No adenoviruses, picobirnaviruses, or Norwalk viruses were found. The rates of virus-associated diarrhea were similar among HIV-positive and HIV-negative children. Enteroviruses were excreted for up to 6 months in HIV-positive children; however, no evidence for prolonged excretion of poliovirus vaccine was observed. These results suggest that although infection with enterovirus and astrovirus may be frequent in HIV-infected children, enteric viruses are not associated with the diarrhea frequently suffered by these children.
Center for Microbiology and Cell Biology, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela.
M B
MB
Liste
I
I
Natera
J A
JA
Suarez
F H
FH
Pujol
F
F
Liprandi
J E
JE
Ludert
D000258:Adenovirus Infections, Human / Q000150:complications / Q000821:virology; D000260:Adenoviruses, Human / Q000302:isolation & purification; D002675:Child, Preschool; D003967:Diarrhea / Q000150:complications / Q000821:virology*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000150:complications* / Q000821:virology; D005243:Feces / Q000821:virology; D015658:HIV Infections / Q000150:complications* / Q000821:virology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D001257:Mamastrovirus / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012327:RNA Virus Infections / Q000150:complications* / Q000821:virology; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification