Galectin-3 decreases in mice exposed to immobilization stress.
Using M3/38 monoclonal antibody we have analyzed effects of immobilization stress on the expression of galectin-3 in liver, spleen and peritoneal macrophages from adult RFM and C3H mice, as well as from aged C3H mice (total of 22 animals). In all analyzed tissues, immobilization stress caused a significant decrease in the expression of galectin-3, ranging from 14% to 47%. The decrease of galectin-3 was observed in both strains of mice, as well as in old animals. Moreover, the same range of decrease (approximately 50%) was observed when cells grown in vitro were exposed to subculturing, or heat-shock. Although the biological significance of this effect is not known, it is very interesting that a single episode of immobilization stress was sufficient to cause a significant decrease in galectin-3, implicating that this beta-galactoside-binding lectin might be involved in the physiological response to psychological stress.
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovacića 1, 10000 Zagreb, Croatia.
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J
Dumić
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K
Barisić
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M
Flögel
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G
Lauc
D000375:Aging / Q000502:physiology*; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism*; D002460:Cell Line; D037502:Galectin 3; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D018869:Heat-Shock Response / Q000502:physiology; D015151:Immunoblotting; D008099:Liver / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D012149:Restraint, Physical; D013154:Spleen / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism*
D000943:Antigens, Differentiation; D037502:Galectin 3