Simultaneous determination of benzodiazepines in whole blood or serum by HPLC/DAD with a semi-micro column.
A simple and sensitive high performance liquid chromatographic (HPLC) method using a semi-micro column, C8 reversed-phase column (3 mm i.d.) and a low flow rate 0.3 ml/min was developed and validated for the determination of five frequently prescribed benzodiazepines: clonazepam, diazepam, flunitrazepam, midazolam and oxazepam. Quantification was performed at 220 nm with methylclonazepam as internal standard. The method involved a simple extraction from alkalinized blood (1 ml) into 1-chlorobutane and provided excellent sensitivity, recovery, accuracy and reproducibility for benzodiazepines in therapeutic or toxic concentrations.
Institut Universitaire de Médecine Légale, Genera, Switzerland.
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El Mahjoub
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Staub
D001569:Benzodiazepines / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D001569:Benzodiazepines