Steroid sulfatase inhibitor alters blood pressure and steroid profiles in hypertensive rats.
Our hypothesis is that the steroid sulfatase gene (Sts) may indirectly contribute to the modulation of blood pressure (BP) in rats with genetic hypertension. The steroid sulfatase enzyme (STS) catalyzes the conversion of estrone sulfate, dehydroepiandrosterone sulfate, cholesterol sulfate and glucocorticoid sulfates to their active nonconjugated forms. This causes the elevation of biologically active steroids, such as glucocorticoids, mineralcorticoids as well as testosterone, which may lead to increased BP. The main objective was to examine the effects of a steroid sulfatase inhibitor on blood pressure and steroid levels in rats with hypertensive genetic backgrounds. Three treatment groups, 5-15 weeks of age were used: controls, estrone and STS inhibitor (estrone-3-O-sulfamate), (n=8 per group). BP was taken weekly by tail cuff, and serum testosterone (T), estrogens (E), and plasma corticosterone (C) levels were measured by radioimmunoassay. BP was significantly reduced by the STS inhibitor in the strains with genetically elevated BP. Also the inhibitor alone significantly reduced plasma corticosterone in all strains compared to estrone treatment with a concomitant as well as significant rise in estrogens and reduction in testosterone and body weight.
Department of Biology, The University of Akron, OH 44325-3908, USA.
S D
SD
Valigora
P K
PK
Lib
G
G
Dunphy
M
M
Turner
D L
DL
Ely
D000818:Animals; D001192:Arylsulfatases / Q000037:antagonists & inhibitors*; D003345:Corticosterone / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology; D004970:Estrone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005260:Female; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D043266:Steryl-Sulfatase; D013739:Testosterone / Q000494:pharmacology
D004791:Enzyme Inhibitors; D004967:Estrogens; C088768:estrone-3-O-sulfamate; D004970:Estrone; D013739:Testosterone; D001192:Arylsulfatases; D043266:Steryl-Sulfatase; D003345:Corticosterone