Thermacetogenium phaeum gen. nov., sp. nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium.
A novel anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium, strain PB(T), was isolated from a thermophilic (55 degrees C) anaerobic methanogenic reactor which had been treating kraft-pulp waste water. The bacterium oxidized acetate in co-culture with a thermophilic hydrogenotrophic methanogen. Strain PB(T), a gram-positive, spore-forming, rod-shaped bacterium grew optimally at 58 degrees C and pH 6.8. The bacterium grew acetogenically on several alcohols, methoxylated aromatics, pyruvate, glycine, cysteine, formate and hydrogen/CO2. Strain PB(T) also oxidized acetate with reduction of sulfate or thiosulfate as the electron acceptor. The bacterium contained MK-7 as the major quinone. The G+C content of the DNA was 53.5 mol%. Comparative 16S rDNA analysis indicated that strain PB(T) belongs to the Bacillus-Clostridium subphylum. However, it was distant from any known genera or micro-organism. The closest known relative was Thermoterrabacterium ferrireducens with 87.4% similarity. The name Thermacetogenium phaeum gen. nov., sp. nov. is proposed. The type strain is strain PBT (= DSM 12270T).
Hattori
S
S
Kamagata
Y
Y
Hanada
S
S
Shoun
H
H
D000085:Acetates / Q000378:metabolism*; D000096:Acetone / Q000378:metabolism; D000438:Alcohols / Q000378:metabolism; D000693:Anaerobiosis; D001407:Bacillus; D001482:Base Composition; D016227:Benzoquinones; D019149:Bioreactors / Q000382:microbiology; D002245:Carbon Dioxide / Q000378:metabolism; D003001:Cloning, Molecular; D018920:Coculture Techniques; D003545:Cysteine / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis; D005561:Formates / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D016936:Gram-Positive Endospore-Forming Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D019289:Pyruvic Acid / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013431:Sulfates / Q000378:metabolism; D013696:Temperature
D000085:Acetates; D000438:Alcohols; D016227:Benzoquinones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005561:Formates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; C030544:formic acid; D000096:Acetone; D002245:Carbon Dioxide; C004532:quinone; D006859:Hydrogen; D019289:Pyruvic Acid; D003545:Cysteine; D005998:Glycine