Fibrous tumors in children - a morphologic and interphase cytogenetic analysis of problematic cases.
We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromosomes 8, 11 and 17. Trisomy 8 and 11 were detected in a high percentage of nuclei in cases of congenital/infantile fibrosarcomas (ranging from 45 to 80%), and in a low grade fibrosarcoma in an older child (23%). Only gains of chromosome 17 were detected in a case of infantile fibromatosis (22%). In this study we have found that given the unconventional histopathologic features, the detection of more than one non-random chromosomal gains by FISH, may aid in further defining fibrous tumors in children, and may be useful as an ancillary diagnostic test in the future.
Flores-Stadler
E M
EM
Department of Pathology, Children's Memorial Hospital, Chicago, IL 60614, USA.
Chou
P M
PM
Barquin
N
N
Thangavelu
M
M
Gonzalez-Crussi
F
F
D000293:Adolescent; D000782:Aneuploidy; D002675:Child, Preschool; D002880:Chromosomes, Human, Pair 11; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D005260:Female; D005350:Fibroma / Q000235:genetics / Q000473:pathology*; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology*; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007231:Infant, Newborn; D007399:Interphase; D008297:Male; D018224:Myofibromatosis / Q000235:genetics / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000473:pathology*; D014314:Trisomy