Effect of media composition on long-term in vitro stability of barium alginate and polyacrylic acid multilayer microcapsules.
For a number of applications stability of microcapsules is a critical factor. Since the maintenance of polyelectrolyte complexes depends considerably on the ion composition we tested the physical properties of barium alginate capsules and searched for conditions to improve stability by a multilayer coating with polyethylenimine (PEI) and polyacrylic acid (PAA). Mechanical stability and diameters were determined in barium alginate capsules and compared with multilayer capsules. Multilayer coating resulted in smaller capsules than barium complexing alone. The difference was more pronounced when CaCl2 was used instead of NaCl during coating. Barium alginate capsules and application of CaCl2 during coating led to continuous pressure profiles, whereas NaCl resulted in bursting at a defined pressure, indicating the additional contribution to mechanical stability by the outer layers. After 7 d culture, mechanical stability of coated capsules decreased in RPMI and NaCl but was most pronounced in sodium citrate. The capsule diameter increased in sodium citrate, less pronounced in NaCl and was significantly different to RPMI and double distilled water. During long-term culture in RPMI, the diameter increased and mechanical stability decreased significantly. Multilayer coating improved mechanical stability which was impeded most in sodium citrate, to a lesser extent by NaCl and RPMI even after long-term exposure.
Institut für Pathologie, Universität Mainz, Germany.
A
A
Gaumann
M
M
Laudes
B
B
Jacob
R
R
Pommersheim
C
C
Laue
W
W
Vogt
J
J
Schrezenmeir
D000180:Acrylic Resins / Q000737:chemistry*; D000464:Alginates / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D002214:Capsules; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003470:Culture Media; D004355:Drug Stability; D010539:Permeability; D011094:Polyethyleneimine; D013314:Stress, Mechanical
D000180:Acrylic Resins; D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D020099:Coated Materials, Biocompatible; D003470:Culture Media; C006903:carbopol 940; D011094:Polyethyleneimine