Mapping of domains on the human parainfluenza type 2 virus P and NP proteins that are involved in the interaction with the L protein.
Eleven monoclonal antibodies (MAbs) directed against the large (L) protein of human parainfluenza type 2 virus (hPIV-2) were prepared to examine the interactions of the L protein with other viral proteins. Coimmunoprecipitation assays using these MAbs revealed that the L protein directly interacted with the phospho- (P) and nucleocapsid (NP) proteins in vivo and in vitro. Mutational analysis of the P or NP protein was performed to identify the region(s) on these proteins interacting with L protein, indicating that amino acids 278-353 on the P protein and amino acids 403-494 on the NP protein are essential for the binding to the L protein.
Department of Microbiology, Mie University School of Medicine, 2-174, Edobashi, Tsu-Shi, Mie-Ken, 514-8507, Japan. nishio@doc.medic.mie-u.ac.jp
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Nishio
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Tsurudome
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Ito
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Ito
D000911:Antibodies, Monoclonal; D001665:Binding Sites; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D009698:Nucleoproteins / Q000737:chemistry* / Q000235:genetics; D010223:Parainfluenza Virus 2, Human; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics; D010957:Plasmids; D011485:Protein Binding; D014162:Transfection; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
D000911:Antibodies, Monoclonal; C407031:NP protein, Human parainfluenza virus 2; D009698:Nucleoproteins; C407030:P protein, Human parainfluenza virus 2; D010750:Phosphoproteins; D014764:Viral Proteins; C069129:L protein, Human parainfluenza virus 2