Lymphocyte 5'-ectonucleotidase: an indicator of oxidative stress in humans?
Lymphocytic 5'-ectonucleotidase (NT) activity previously has been shown to be reduced in patients with a high pro-oxidant state. This study shows that NT activity is decreased in vitro by exposure to superoxide anions and that ascorbate protects against this effect. Also, a putative high pro-oxidant state in vivo, as indicated by low tissue ascorbate levels, resulted in a significant decrease in NT which an antioxidant intake normalized. These results taken together suggest that NT is sensitive to superoxide anion and that it may be a good marker of a pro-oxidant state in humans.
Chalmers
A H
AH
Department of Haematology and Genetic Pathology, Flinders University Medical Centre, Bedford Park, South Australia, Australia. chalmers.ah@flinders.edu.au
Blake-Mortimer
J S
JS
Winefield
A H
AH
D015720:5'-Nucleotidase / Q000097:blood*; D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use; D015415:Biomarkers / Q000097:blood; D003866:Depressive Disorder / Q000097:blood*; D019587:Dietary Supplements; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D018384:Oxidative Stress; D012016:Reference Values; D013315:Stress, Psychological / Q000097:blood*; D013481:Superoxides / Q000097:blood / Q000494:pharmacology
D015720:5'-Nucleotidase; D000975:Antioxidants; D015415:Biomarkers; D006442:Glycated Hemoglobin; D013481:Superoxides