Glycosylphosphatidylinositol-specific phospholipase D of human serum--activity modulation by naturally occurring amphiphiles.
The enzymatic properties of glycosylphosphatidylinositol-specific phospholipase D (EC 3.1.4.50) were characterized using a 6,000-fold purified enzyme. This was obtained in 100 microg amounts from human serum with a recovery of 35%. Pure alkaline phosphatase containing one anchor moiety per molecule was used as substrate. The enzyme is stimulated by n-butanol, but in contrast to other phospholipases this activation is not produced by a transphosphatidylation reaction. The previously reported non-linearity of the specific activity with respect to phospholipase concentration in the test was no longer observed upon purification, indicating inhibitor removal. The serum inhibitor(s) co-chromatograph with serum proteins and lipoproteins. The main part of the inhibitory activity was found in the lipid fraction after protein denaturation and can be subfractionated into acid phospholipids, cholesteryl esters and triacylglycerides. Added phosphatidyl-serine, phosphatidylinositol, phosphatidylglycerol, gangliosides, cholesteryl esters, and sphingomyelins turned out to be strong inhibitors, as well as phosphatidic acid. Phosphatidylethanolamine and various monoacylglycerols were found to be activators. The low glycosylphosphatidylinositol-specific phospholipase activity found in native serum did not increase significantly upon 90% removal of phospholipids by n-butanol. High serum concentrations of strongly inhibiting compounds, complex kinetic interactions among aggregates of these substances, and compartmentalization effects are discussed as possible reasons for the observed inactivity.
Institute of Biochemistry, Medical Faculty, Friedrich Schiller University, Jena, Germany.
H
H
Rhode
M
M
Schulze
G A
GA
Cumme
A
A
Göhlert
E
E
Blume
R
R
Bublitz
K
K
Schilling
A
A
Horn
D020001:1-Butanol / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D017830:Octoxynol; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000097:blood*; D013379:Substrate Specificity
D020001:1-Butanol; D017830:Octoxynol; D010739:Phospholipase D; C065017:glycoprotein phospholipase D