Interleukin-18 modulates immune responses induced by HIV-1 Nef DNA prime/protein boost vaccine.
Many different HIV-1 vaccine strategies have been developed, but as yet none has been completely successful. Promising results from combined DNA prime/protein boost vaccines have been reported. Specific immune responses generated by DNA vaccines can be modulated by the co-delivery of genes coding for cytokines. In this study, we have used the intradermal route by needle injection of a plasmid coding for the HIV-1 Nef accessory protein. We show that DNA prime/protein boost vaccine combinations increase the humoral and cellular immune responses against HIV-1 Nef and that the co-injection of DNA encoding Interleukin-18 (IL-18) modulates the specific immune response towards a Th1 type.
Unité Inserm 167, Pasteur de Lille, Lille, France.
O
O
Billaut-Mulot
T
T
Idziorek
E
E
Ban
L
L
Kremer
L
L
Dupré
M
M
Loyens
G
G
Riveau
C
C
Locht
A
A
Capron
G M
GM
Bahr
D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D004279:DNA, Viral / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D015693:Gene Products, nef / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular / Q000187:drug effects; D007117:Immunization, Secondary; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology; D054311:nef Gene Products, Human Immunodeficiency Virus
D016915:AIDS Vaccines; D004279:DNA, Viral; D015693:Gene Products, nef; D020382:Interleukin-18; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014764:Viral Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus