Characterization of a binding site of UCN-01, a novel anticancer drug on alpha-acid glycoprotein.
The binding site of 7-hydroxystaurosporine (UCN-01) on alpha-acid glycoprotein (AGP) was studied by fluorescence and ultracentrifugation experiments. Three ligands, propranolol, warfarin and progesterone were employed as marker ligands and quinaldine red was employed as a fluorescent probe. The presence of UCN-01, pro- pranolol, warfarin and progesterone resulted in a significant quenching of the fluorescence of quinaldine red, when bound to AGP, depending upon the potency of the binding to AGP. The construction of Klotz plots indicated that the displacement effects of propranolol, warfarin and progesterone on UCN-01-AGP binding were competitive in nature. These data suggest that the binding site of UCN-01 on the AGP partly overlaps the binding site for basic drugs, acidic drugs, as well as steroid hormones.
Pharmaceutical Research Institute, Kyowa Hakko Kogyo Co, Ltd, Sunto-gun, Shizuoka, Japan,
N
N
Kurata
S
S
Matsushita
K
K
Nishi
H H
HH
Watanabe
S
S
Kobayashi
A
A
Suenaga
M
M
Otagiri
D000470:Alkaloids / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism*; D001667:Binding, Competitive; D009961:Orosomucoid / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism; D019311:Staurosporine / Q000031:analogs & derivatives; D014461:Ultracentrifugation; D014859:Warfarin / Q000378:metabolism
D000470:Alkaloids; D000970:Antineoplastic Agents; D009961:Orosomucoid; D011374:Progesterone; D014859:Warfarin; C054852:7-hydroxystaurosporine; D011433:Propranolol; D019311:Staurosporine