[Comparative study of expression levels of the major human heat shock proteins in cancer and normal tissues].
OBJECTIVE
To investigate and compare mRNA expression levels of the major human HSPs (including HSP90 alpha, HSP90 beta, HSP70, HSC70 and HSP27) in 25 cases of laryngeal carcinomas and normal controls.


METHODS
mRNA slot blot hybridization technique was used and cDNA probes of HSP27, HSC70 and HSP90 beta were prepared with PCR method.


RESULTS
HSP90 alpha and HSP70 expression was significantly enhanced approximately 5 fold in laryngeal cancer tissues compared to that in the normal larynx tissues while HSP27, HSC70 and HSP90 beta expression was at similar levels.


CONCLUSIONS
The results suggest that HSP90 alpha and HSP70 may play more important role in the carcinogenic process of laryngeal cancer. To find out mechanisms involved will pave the way for the application of HSPs on the biologic therapy of laryngeal cancer.
Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Y
Y
Shi
M
M
Zhao
X
X
Xu
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015870:Gene Expression; D050883:HSC70 Heat-Shock Proteins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics
D002352:Carrier Proteins; D050883:HSC70 Heat-Shock Proteins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; C494371:HSPA8 protein, human; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D012333:RNA, Messenger