Effects of new polymer-coated extracorporeal circuits on biocompatibility during cardiopulmonary bypass.
An inflammatory response due to bioincompatibility of extracorporeal circuits is a major clinical issue during cardiopulmonary bypass (CPB). By using a swine model, we determined whether new polymer-coated circuits, the blood-contacting surfaces of which are coated with poly(2-methoxyethylacrylate) (PMEA), would reduce the inflammatory response during CPB. Plasma bradykinin levels and the percentages of CD35-positive monocytes in PMEA-coated circuits were significantly lower than those in uncoated circuits during CPB. The amount of proteins adsorbed on the PMEA-coated circuits was significantly lower than that on the uncoated circuits (0.30 microg/cm2 versus 3.42 microg/ cm2). Almost no IgG, IgM, or C3c/d was detected in proteins adsorbed on the PMEA-coated circuits although these proteins were clearly detected in proteins adsorbed on the uncoated circuits. We concluded that PMEA coating could reduce complement activation during CPB by suppressing the adsorption of IgG and IgM, which activate C3 via the classical pathway, on the surface of the circuits.
Terumo Corporation R&D Center, Biological Evaluation Center, Kanagawa, Japan.
N
N
Saito
S
S
Motoyama
J
J
Sawamoto
D000179:Acrylates; D000327:Adsorption; D000704:Analysis of Variance; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D001920:Bradykinin / Q000097:blood; D002315:Cardiopulmonary Bypass / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible; D015932:Complement C3c / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000097:blood; D005434:Flow Cytometry; D006493:Heparin; D015151:Immunoblotting; D007249:Inflammation / Q000517:prevention & control*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D011108:Polymers; D011863:Radioimmunoassay; D017463:Receptors, Complement 3b / Q000097:blood; D013552:Swine
D000179:Acrylates; D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D004731:Endotoxins; D018950:Lipopolysaccharide Receptors; D011108:Polymers; D017463:Receptors, Complement 3b; C413485:poly(2-methoxyethylacrylate); D015932:Complement C3c; D006493:Heparin; D001920:Bradykinin