Optimised extraction of folic acid from multivitamin-mineral preparations for liquid chromatographic analysis.
Degradation of folic acid may occur during extraction of multivitamin-mineral preparations. The degradation may be caused by presence of ions such as Fe3+ and Cu2+, however, the buffer composition may also be critical. This study presents an optimised extraction procedure tested on 24 different products of multivitamin-mineral tablets. The present method yielded mean recoveries of 97% (n = 20) for folic acid and prevented degradation of folic acid in at least 24 h in extracts from multivitamin-mineral tablets.
Danish Veterinary and Food Administration, Institute of Food Research and Nutrition, Ministry of Food, Agriculture and Fisheries, Søborg. mak@fdir.dk
M A
MA
Kall
P
P
Nørgaard
S J
SJ
Pedersen
T
T
Leth
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005492:Folic Acid / Q000302:isolation & purification*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014815:Vitamins / Q000737:chemistry*
D004364:Pharmaceutical Preparations; D014815:Vitamins; D005492:Folic Acid