[Detection of epidermal growth factor receptor gene amplification in human laryngeal carcinomas by means of fluorescence in situ hybridization].
OBJECTIVE
To detect epidermal growth factor receptor(EGFR) gene amplification in human laryngeal carcinoma Hep-2 cell line and laryngeal carcinoma tissues.


METHODS
The technique of fluorescence in situ hybridization(FISH).


RESULTS
The EGFR gene amplification of a laryngeal carcinoma Hep-2 cell line and 5 laryngeal carcinoma tissues were detected by FISH. In the metaphase chromosome and interphase nuclei of Hep-2 cell line and 2 laryngeal carcinoma tissues, distinct cluster and multiple dot signals were found. In the interphase nuclei of the other 3 laryngeal carcinoma tissues, no increase in the number or extent of the hybrid signals was found.


CONCLUSIONS
Compared with normal diploid cell, the EGFR gene amplification was observed at different levels ranging from 2 to 8 folds in metaphase chromosome and interphase nuclei of Hep-2 cell line and 2 laryngeal carcinoma tissues while no amplification was observed in the other 3 laryngeal carcinoma tissues. The results demonstrate that quantitative detection of amplified gene by FISH in the metaphase chromosome and interphase nuclei is useful for detection of laryngeal carcinoma tissues.
Department of Medical Genetics, China Medical University, Shenyang, Liaoning, P. R. China. klsun@mail.cmu.edu.cn
F
F
Li
N
N
Kang
W
W
Fu
X
X
Sun
H
H
Gao
K
K
Sun
D066246:ErbB Receptors / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007822:Laryngeal Neoplasms / Q000235:genetics*
D066246:ErbB Receptors