Distinct P-element excision products in somatic and germline cells of Drosophila melanogaster.
The footprints remaining following somatic P-element excision from the Drosophila white locus were recovered and characterized. Two different types of footprints were observed. Over 75% of the footprints were short, composed of 4 or 7 nucleotides of the P-element inverted terminal repeat, and were similar to those found in a previously described plasmid excision assay. The remaining footprints were composed of 14-18 nucleotides of both inverted terminal repeats. These large footprints were indistinguishable from those recovered following germline P-element excision. Enhanced expression of the Drosophila homologue of the Ku70 protein did not affect the structure of the somatic footprints. Therefore, this protein is not a limiting factor for double-strand break repair by nonhomologous end-joining in Drosophila somatic cells.
Department of Biochemistry, Faculty of Medicine and Dentistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada. ggloor@julian.uwo.ca
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GB
Gloor
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Moretti
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Mouyal
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KJ
Keeler
D000818:Animals; D034961:Antigens, Nuclear; D002842:Chromatids / Q000378:metabolism; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004265:DNA Helicases; D004260:DNA Repair; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005091:Exons; D005785:Gene Conversion; D005854:Germ Cells; D007438:Introns; D000072200:Ku Autoantigen; D008957:Models, Genetic; D009687:Nuclear Proteins / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA
D034961:Antigens, Nuclear; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen