Sensitive analysis of apoptosis using confocal laser scan microscopy.
Application of the TUNEL method and immunostaining cell-specific markers to a whole isolated glomerulus in combination with confocal laser scan microscopy can be used to analyze cell turnover including apoptosis within glomeruli. Furthermore, the technologies can be used to deepen our understanding of glomerular cell biology and pathophysiology at the cellular and molecular levels.
Department of Cellular Physiology, Institute of Nephrology, Niigata University School of Medicine, Niigata, Japan.oite@med.niigata-u.ac.jp
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D000818:Animals; D017209:Apoptosis; D053938:DNA Fragmentation; D005455:Fluorescent Antibody Technique; D020287:In Situ Nick-End Labeling; D007678:Kidney Glomerulus / Q000166:cytology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D051381:Rats