Simultaneous intravascular two-dimensional plus Doppler ultrasound is useful for evaluation of postischemic vasodilatation: plasm NOx - levels determined by the Griess method may not reflect the extent of postischemic NO-induced vasodilatation.
Our objective was 1) to assess postischemic vasodilatation using simultaneous intravascular two-dimensional and Doppler ultrasound, and 2) to clarify whether plasma nitrite and nitrate (NOx-) levels change during postischemic vasodilatation. The vascular cross-sectional area (CSA) was evaluated in 18 mongrel dogs, and the average instantaneous peak velocity (APV) in the iliac arteries after the 5-min occlusion of blood flow was determined. Plasma NOx- levels were measured at the baseline, during the occlusion of blood flow, and 1.5, 3, and 10 min after recanalization. The %CSA significantly increased from 30 s to 7 min after the recanalization, and maximal vasodilatation was observed at 1.5 min after the recanalization (14.1 +/- 0.9 to 15.8 +/- 1.0 mm2, p< 0.0001 vs. baseline). Plasma NOx- levels were significantly reduced during the occlusion of blood flow and remained reduced at 1.5, 3, and 10 min after recanalization compared with the baseline values. We concluded that simultaneous intravascular two-dimensional and Doppler ultrasound is useful for assessment during postischemic vasodilatation, and that plasma NOx- levels assayed with the Griess reagent do not significantly increase, even when maximal vasodilatation is observed.
Hayakawa
M
M
Internal Medicine II, Nagoya University School of Medicine, Japan.
Ito
T
T
Osanai
H
H
Harada
M
M
Numaguchi
Y
Y
Mokuno
S
S
Murase
K
K
Toki
Y
Y
Okumura
K
K
Hayakawa
T
T
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002309:Cardiology / Q000379:methods; D003331:Coronary Vessels / Q000000981:diagnostic imaging / Q000187:drug effects / Q000503:physiopathology; D004285:Dogs; D004452:Echocardiography; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D007269:Injections, Intra-Arterial; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000503:physiopathology*; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000502:physiology; D009573:Nitrites / Q000097:blood; D013997:Time Factors; D018084:Ultrasonography, Interventional; D014664:Vasodilation; D019323:omega-N-Methylarginine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D009566:Nitrates; D009573:Nitrites; D019323:omega-N-Methylarginine; D009569:Nitric Oxide