Use of 2-methylpropan-2-ol to inhibit proteolysis and other protein interactions in a tissue homogenate: an illustrative application to the extraction of cathepsins B and L from liver tissue.
School of Molecular and Cellular Biosciences, University of Natal, Pietermaritzburg 3209, South Africa. dennison@unpsun1.cc.unp.ac.za
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Dennison
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Moolman
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Pillay
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Meinesz
D000818:Animals; D001963:Bromelains / Q000378:metabolism; D002401:Cathepsin B / Q000302:isolation & purification* / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D056668:Cathepsin L; D002403:Cathepsins / Q000302:isolation & purification* / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D003546:Cysteine Endopeptidases; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases; D008099:Liver / Q000737:chemistry*; D011817:Rabbits; D012685:Sepharose / Q000494:pharmacology; D012756:Sheep; D014357:Trypsin / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism; D020002:tert-Butyl Alcohol / Q000494:pharmacology
D001963:Bromelains; D012685:Sepharose; D000520:alpha-Glucosidases; D002403:Cathepsins; D010450:Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; D002402:Cathepsin D; D020002:tert-Butyl Alcohol