Determination of divalent carboxylic acids in traditional Chinese herbal and patent medicines using HPLC with double cell quartz crystal detector.
A rapid, simple and sensitive high performance liquid chromatography (HPLC) method combined with a novel double cell quartz crystal (DCQC) detector is described for the determination of divalent carboxylic acids in traditional Chinese herbal and patent medicines. The chromatographic system involves the use of a muBondapak-NH2 column with 20 mM KH2PO4 (pH 3.4, adjusted by H3PO4)/acetonitrile (95:5%) as a mobile phase. The DCQC detector has a low temperature coefficient and high conductance sensitivity independent of the background from 7.2 to 2500 microS. Analysis of sample is completed without the use of an ion suppression device.
New Materials Research Institute, Hunan University, Changsha, People's Republic of China. chen-po@163.net
P
P
Chen
Y
Y
Zhang
W
W
Wei
S Z
SZ
Yao
D002138:Calibration; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011791:Quartz; D013057:Spectrum Analysis / Q000379:methods*
D002264:Carboxylic Acids; D004365:Drugs, Chinese Herbal; D004364:Pharmaceutical Preparations; D011791:Quartz