Determinants of Mg2+-dependent activities of recombinant human immunodeficiency virus type 1 integrase.
The relationship between Mg(2+)-dependent activity and the self-assembly state of HIV-1 integrase was investigated using different protein preparations. The first preparations, IN(CHAPS) and IN(dial), were purified in the presence of detergent, but in the case of IN(dial), the detergent was removed during a final dialysis. The third preparation, IN(zn), was purified without any detergent. The three preparations displayed comparable Mn(2+)-dependent activities. In contrast, the Mg(2+)-dependent activity that reflects a more realistic view of the physiological activity strongly depended on the preparation. IN(CHAPS) was not capable of using Mg(2+) as a cofactor, whereas IN(zn) was highly active under the same conditions. In the accompanying paper [Deprez, E., et al. (2000) Biochemistry 39, 9275-9284], we used time-resolved fluorescence anisotropy to demonstrate that IN(CHAPS) was monomeric at the concentration of enzymatic assays. Here, we show that IN(zn) was homogeneously tetrameric under similar conditions. Moreover, IN(dial) that exhibited an intermediary Mg(2+)-dependent activity existed in a monomer-multimer equilibrium. The level of Mg(2+)- but not Mn(2+)-dependent activity of IN(dial) was altered by addition of detergent which plays a detrimental role in the maintenance of the oligomeric organization. Our results indicate that the ability of integrase to use Mg(2+) as a cofactor is related to its self-assembly state in solution, whereas Mn(2+)-dependent activity is not. Finally, the oligomeric IN(zn) was capable of binding efficiently to DNA regardless of the cationic cofactor, whereas the monomeric IN(CHAPS) strictly required Mn(2+). Thus, we propose that a specific conformation of integrase is a prerequisite for its binding to DNA in the presence of Mg(2+).
Laboratoire de Physicochimie et de Pharmacologie des Macromolécules Biologiques (UMR-CNRS 8532), Villejuif Cedex, France.
H
H
Leh
P
P
Brodin
J
J
Bischerour
E
E
Deprez
P
P
Tauc
J C
JC
Brochon
E
E
LeCam
D
D
Coulaud
C
C
Auclair
J F
JF
Mouscadet
D002793:Cholic Acids / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D004789:Enzyme Activation / Q000235:genetics; D019427:HIV Integrase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D006801:Humans; D008274:Magnesium / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility; D013379:Substrate Specificity / Q000235:genetics; D016662:Virus Integration / Q000235:genetics; D015032:Zinc / Q000737:chemistry
D002793:Cholic Acids; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D003902:Detergents; D011993:Recombinant Fusion Proteins; D008345:Manganese; D019427:HIV Integrase; D008274:Magnesium; D015032:Zinc; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate