Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide.
The conformational changes in alpha-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, alpha-helix content decreased to 37%, while without the filter it was 68%. The alpha-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.
Venture Business Laboratory, Kyushu University, Fukuoka, Japan. ishikawa@agr.kyushu-u.ac.jp
H
H
Ishikawa
M
M
Shimoda
A
A
Yonekura
K
K
Mishima
K
K
Matsumoto
Y
Y
Osajima
D002245:Carbon Dioxide; D002942:Circular Dichroism; D066298:In Vitro Techniques; D011099:Polyglutamic Acid / Q000737:chemistry*; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary
D002245:Carbon Dioxide; D011099:Polyglutamic Acid