[Flow cytometry, basic principles and applications--with special reference to pediatrics].
Flow cytometry in clinical applications and research is the result of knowledge and techniques concerning cyto and fluorochemistry, laser technology, monoclonal antibodies and computer processing. Flow cytometry can assess multiple physical and biological cell properties, in a relatively short period of time. It is specially important, that specimens can concern different types of material: fresh solid tissue, embedded in paraffin, blood, bone marrow, body fluids. Under optimal conditions (standarization of method and quality control) precise and objective analysis of a large number of cells is provided. An experienced team and cooperation with clinicians and pathologists are indispensable for correct clinical interpretation of flow cytometry data. The aim of this study mainly concerns: general principles of flow cytometry operation, clinical applications--especially in paediatrics. It is also an encouragement to use this valuable and modern instrument in clinical and research work.
Rytwiński
K
K
Samodzielna Pracownia Cytometrii, Instytut Matki i Dziecka w Warszawie.
Wnuk
A
A
D000293:Adolescent; D015415:Biomarkers / Q000032:analysis; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000473:pathology; D010372:Pediatrics / Q000379:methods*; D012107:Research Design; D014652:Vascular Diseases / Q000175:diagnosis
D015415:Biomarkers; D004273:DNA, Neoplasm