[Antisense N-ras1 gene inhibits the proliferation of VSMC induced by bFGF after arterial injury].
OBJECTIVE
To develop a new way to prevent the process of restenosis after arterial injury, we transfer the recombinant antisense N-ras1 gene to the cultured SMCs and studied the influence of gene therapy with antisense N-ras1 on the SMCs proliferation induced by bFGF.


METHODS
With the recombinant antisense N-ras1 plasmids (fpGv1-MT-N-ras1) transfered to the cultured SMC, we studied the effect on the SMCs proliferation induced by bFGF.


RESULTS
The results of the experiment indicated that the count of cells transfered antisense N-ras1 was 16.8 +/- 1.3 x 10(4), the control cells transfered fpGv1-MT retrovirus vector were 30.1 +/- 1.2 x 10(4) (cell/ml) (P < 0.001). cell [3H] thymedine incorporation was 7,643 +/- 672 cpm in gene therapy cells, and 15,131 +/- 138 cpm in vector control cells (P < 0.001). Gene therapy cells ras mRNA and p21 were significantly decreased by Northern blotting and Western blotting technique.


CONCLUSIONS
These results suggest that antisense N-ras1 gene may inhibit the proliferation of cultured VSMC induced by bFGF.
Department of Cardiology, First Hospital, Beijing Medical University.
Y
Y
Zhou
L
L
Wang
A
A
Zhou
D000818:Animals; D016375:Antisense Elements (Genetics); D002455:Cell Division; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D018014:Gene Transfer Techniques; D011905:Genes, ras; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010957:Plasmids; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D018631:ras Proteins / Q000096:biosynthesis
D016375:Antisense Elements (Genetics); D016222:Fibroblast Growth Factor 2; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins