Identification of type 4 fimbriae in Actinobacillus pleuropneumoniae.
Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N-terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins.
Zhang
Y
Y
CSIRO Animal Health, Australian Animal Health Laboratory, Private Bag 24, 3220, Geelong, Vic., Australia. yamei.zhang@dah.csiro.au
Tennent
J M
JM
Ingham
A
A
Beddome
G
G
Prideaux
C
C
Michalski
W P
WP
D016977:Actinobacillus pleuropneumoniae / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D010861:Fimbriae, Bacterial / Q000145:classification / Q000235:genetics / Q000648:ultrastructure*; D008854:Microscopy, Electron; D017422:Sequence Analysis, DNA
D001425:Bacterial Outer Membrane Proteins