Synthesis of interleukin 1beta and interleukin 6 by stimulated rat peritoneal macrophages: modulation by glutamine.
Synthesis and secretion of IL-1beta and IL-6 were compared in LPS-stimulated rat peritoneal macrophages, and the effect of glutamine studied. LPS induced a parallel increase in mRNA and synthesis of IL-1beta and IL-6. IL-1beta accumulated mainly in the cytosol and IL-6 in the culture medium. Glutamine addition increased the synthesis of both cytokines, but the overall production (intra-+extracellular) of IL-1beta increased two-fold, although that of IL-6 increased only 1.3-fold. The influence of glutamine is discussed.
Groupe de Biochimie et Physiopathologie Digestive et Nutritionnelle (GBPDN), Institut Fédératif de Recherches Multidisciplinaires sur les Peptides No 23 (IFRMP), UFR Médecine-Pharmacie de Rouen, France.
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Yassad
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Husson
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Bion
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Lavoinne
D000818:Animals; D005973:Glutamine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D005973:Glutamine