Membrane permeability modifications are involved in antibiotic resistance in Klebsiella pneumoniae.
Two Klebsiella pneumoniae strains selected according to their high cross-resistance pattern to cephalosporins were characterized by (i) outer membrane protein content such as OmpA or nonspecific porins, (ii) MICs of various cephalosporins and unrelated antibiotics, (iii) beta-lactamase production, and (iv) active efflux of fluoroquinolones. An association of porin deficiency and beta-lactamase production induced a noticeable cephalosporin resistance. In addition to these mechanisms, the presence of an active efflux participating in high-level fluoroquinolone resistance was identified in one strain. The decrease of antibiotic uptake associated with efflux explains the Klebsiella adaptation against the drugs present in the environment.
Chevalier
J
J
Faculté de Médecine, CJF 9606 INSERM, Université de la Méditerranée, 27 Boulevard Jean Moulin, Marseille Cedex 05, 13385, France.
Pagès
J M
JM
Eyraud
A
A
Malléa
M
M
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D004352:Drug Resistance, Microbial / Q000502:physiology; D015378:Imipenem / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009643:Norfloxacin / Q000493:pharmacokinetics; D001618:beta-Lactamases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001425:Bacterial Outer Membrane Proteins; D015378:Imipenem; D001618:beta-Lactamases; D009643:Norfloxacin