Lens culinaris, Phaseolus vulgaris and vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line CACO-2.
Cultured Caco-2 cells were stimulated with Lens culinaris, Phaseolus vulgarisandVicia fabalectins. The production of IL-1, IL-6, IL-8 and MCP-1 was measured by ELISA and RT-PCR. IL-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the other cytokines tested were produced. The IL-8 secreted may induce the extravasation of activated neutrophils and generate tissue damage. A similar mechanism may be implicated in the lesions observed after infection by some enteric pathogens, with lectin-like domains on their membrane. Finally, this model may be suitable one to study the regulation of IL-8 production.
Rodríguez-Juan
C
C
Immunología, Universidad Complutense de Madrid, Madrid, Spain.
Pérez-Blas
M
M
Suárez-García
E
E
López-Suárez
J C
JC
Múzquiz
M
M
Cuadrado
C
C
Martín-Villa
J M
JM
D018938:Caco-2 Cells; D018932:Chemokine CCL2 / Q000378:metabolism; D003106:Colon / Q000187:drug effects* / Q000378:metabolism; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D037121:Plant Lectins; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018932:Chemokine CCL2; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D037102:Lectins; D010835:Phytohemagglutinins; D037121:Plant Lectins; C042242:Vicia lectins; C033619:lentil lectin