Dependence of stem cell fate in Arabidopsis on a feedback loop regulated by CLV3 activity.
The fate of stem cells in plant meristems is governed by directional signaling systems that are regulated by negative feedback. In Arabidopsis thaliana, the CLAVATA (CLV) genes encode the essential components of a negative, stem cell-restricting pathway. We used transgenic plants overexpressing CLV3 to show that meristem cell accumulation and fate depends directly on the level of CLV3 activity and that CLV3 signaling occurs exclusively through a CLV1/CLV2 receptor kinase complex. We also demonstrate that the CLV pathway acts by repressing the activity of the transcription factor WUSCHEL, an element of the positive, stem cell-promoting pathway.
Institut für Entwicklungsbiologie, Universität zu Köln, Gyrhofstrabetae 17, D-50923 Köln, Germany.
U
U
Brand
J C
JC
Fletcher
M
M
Hobe
E M
EM
Meyerowitz
R
R
Simon
D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins; D002454:Cell Differentiation; D002455:Cell Division; D015536:Down-Regulation; D005246:Feedback; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000378:metabolism; D018519:Meristem / Q000166:cytology* / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014157:Transcription Factors; D019076:Transgenes
C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; C401621:CLV2 protein, Arabidopsis; D018398:Homeodomain Proteins; D008565:Membrane Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014157:Transcription Factors; C098247:UFO protein, Arabidopsis; C116421:WUSCHEL protein, Arabidopsis; D020794:Receptor Protein-Tyrosine Kinases; C118166:CLV1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases