Effect of strenuous arm exercise on oxidized-LDL-potentiated platelet activation in individuals with spinal cord injury.
This study investigates how strenuous arm exercise affects oxidized-low density lipoprotein (O(X)-LDL) mediated-platelet activation in patients with SCI. Ten patients with SCI and ten age- and sex-matched healthy subjects exercised strenuously using an arm crank ergometer. The following measurements were taken both when the subjects were at rest, and immediately after exercise: plasma lipid profile, O(X)-LDL mediated platelet aggregability and [Ca(2+)]i, urinary 11-dehydro-thromboxane B2 (11-dehydro-TXB2) and 8-iso-prostaglandin F(2alpha), (8-iso-PG F(2alpha)) contents, and plasma NO metabolite (nitrite plus nitrate) level. Based on these measurements, the major findings of this study can be summarized as follows: 1) the SCI group had higher urinary 8-iso-PGF(2alpha) and 11-dehydro-TXB2 contents, but a lower plasma nitrite plus nitrate level than the control group; 2) at rest, the SCI group had a higher platelet aggregability and [Ca(2+)]i, and O(X)-LDL-potentiated platelet activation than the control group; 3) O(X)-LDL-potentiated platelet aggregation was enhanced by strenuous arm exercise in both groups, but the effect of exercise was more pronounced in the SCI group than in the control group; 4) treating the platelet with L-arginine inhibited O(X)-LDL-potentiated platelet activation in both groups. The study concludes that individuals with SCI had more extensive resting and exercise-enhanced O(X)-LDL-potentiated platelet activation and greater amounts of preformed lipid peroxides than those without SCI. Therefore, supplementation therapy with antioxidants may be needed for patients with SCI, especially in a strenuous arm exercise period.
Wang
J S
JS
Department of Physical Therapy, Chang Gung University, Taoyun, Tawan. s5492@mail.cgu.edu.tw
Yang
C F
CF
Wong
M K
MK
Chow
S E
SE
Chen
J K
JK
D000328:Adult; D001132:Arm / Q000502:physiology*; D001161:Arteriosclerosis / Q000453:epidemiology; D002118:Calcium / Q000097:blood; D002318:Cardiovascular Diseases / Q000453:epidemiology; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D005080:Exercise Test; D028441:F2-Isoprostanes; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000502:physiology*; D008297:Male; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000502:physiology; D009573:Nitrites / Q000097:blood; D018384:Oxidative Stress; D015539:Platelet Activation; D010974:Platelet Aggregation; D010976:Platelet Count; D012307:Risk Factors; D013119:Spinal Cord Injuries / Q000097:blood*; D013929:Thromboxane B2 / Q000031:analogs & derivatives / Q000652:urine
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D028441:F2-Isoprostanes; D008077:Lipoproteins, LDL; D009566:Nitrates; D009573:Nitrites; C099252:oxidized low density lipoprotein; C075750:8-epi-prostaglandin F2alpha; D009569:Nitric Oxide; D013929:Thromboxane B2; C049235:11-dehydro-thromboxane B2; D015237:Dinoprost; D002118:Calcium