Production of Poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution.
Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the A. latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity of 2.57 g/liter/h.
Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Yusong-gu, Taejon 305-701, Korea.
W S
WS
Ahn
S J
SJ
Park
S Y
SY
Lee
D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000421:Alcaligenes / Q000235:genetics; D000818:Animals; D002417:Cattle; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D006885:Hydroxybutyrates / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D008894:Milk Proteins / Q000378:metabolism*; D010957:Plasmids; D011091:Polyesters / Q000378:metabolism*; D014169:Transformation, Bacterial; D000067816:Whey Proteins; D000094122:Polyhydroxybutyrates
D000217:Acyltransferases; D003470:Culture Media; D006885:Hydroxybutyrates; D007785:Lactose; D008894:Milk Proteins; D011091:Polyesters; D000067816:Whey Proteins; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C077225:poly(3-hydroxyalkanoic acid) synthase