Photoinduced graft polymerization of 2-methacryloyloxyethyl phosphorylcholine on polyethylene membrane surface for obtaining blood cell adhesion resistance.
Phospholipid polymer, poly[2-methacryloyloxyethyl phosphorylcholine (MPC)], was grafted with polyethylene (PE) membrane using photoinduced polymerization technique to make the membrane resistant to cell adhesion. The water contact angle on the PE membrane grafted with poly(MPC) decreased with an increase in the photopolymerization time. This decrease corresponded to the increase in the amount of poly(MPC) grafted on the PE surface. The same graft polymerization procedure was applied using other hydrophilic monomers, such as acrylamide (AAm), N-vinylpyrrolidone (VPy) and methacryloyl poly(ethylene glycol) (MPEG). These monomers were also polymerized to form grafted chains on the PE membrane, and the grafting was confirmed with X-ray photoelectron spectroscopy. Analysis of amount and distribution of plasma proteins at the plasma-contacting surface of the original and the modified PE membranes were analyzed using immunogold assay. The grafting of poly(MPC) and poly(VPy) on PE membrane reduced the plasma protein adsorption significantly compared with that on the original PE membrane. However, the PE membranes grafted with poly(AAm) or poly(MPEG) did not show any effects on protein adsorption. Platelet adhesion on the original and modified PE membranes from platelet-rich plasma was also examined. A large number of platelets adhered and activated on the original PE membrane. Grafting with poly(AAm) did not suppress platelet adhesion, but grafting with poly(MPC) or poly(VPy) on the PE membrane was effective in preventing platelet adhesion. It is concluded that the introduction of the phosphorylcholine group on the surface could decrease the cell adhesion to substrate polymer.
Department of Materials Science, Graduate School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, 113-8656, Tokyo, Japan
K
Ishihara
Y
Iwasaki
S
Ebihara
Y
Shindo
N
Nakabayashi