[The effect of antibody against vascular endothelial growth factor on tumor growth].
OBJECTIVE
Vascular endothelial growth factor (VEGF) is a very important factor in the process of angiogenesis, which is chosen as a target for experimental study of anticancer.


METHODS
Anti-VEGF antibody was obtained from immunized rabbits and purified with affinity column. Morever, it was identified as neutralized antibody by Mile's assay.


RESULTS
The anti-VEGF antibody was able to inhibit growth of S180 sarcoma in a dose-dependent manner, and the inhibition rate reached 41.0% with dose 200 micrograms per mouse per day. For human gastric cancer (MGC-803)-bearing nude mice, Anti-VEGF antibody inhibited tumor growth by 76.2%. When anti-VEGF antibody was combined with 131I-3H11, a murine monoclonal antibody conjugated with 131I for treatment, only one of five nude mice developed tumor and 84.0% more inhibition of tumor growth was obtained in comparison with treatment by 131I-3H11 only.


CONCLUSIONS
The anti-VEGF antibody may be potentially useful for clinical treatment of cancer.
Beijing Institute for Cancer Research, Beijing Medical University.
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Wang
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Wang
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Liu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D002455:Cell Division; D016228:Endothelial Growth Factors / Q000276:immunology*; D006801:Humans; D000922:Immunotoxins / Q000627:therapeutic use*; D007457:Iodine Radioisotopes / Q000627:therapeutic use; D008222:Lymphokines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012510:Sarcoma 180 / Q000473:pathology / Q000628:therapy*; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D000911:Antibodies, Monoclonal; D016228:Endothelial Growth Factors; D000922:Immunotoxins; D007457:Iodine Radioisotopes; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors