[Treatment of chronic wounds with cultured autologous keratinocytes as suspension in fibrin glue].
Cultivated keratinocytes have been used for treatment of chronic wounds. Our group developed a new application form, using a suspension of subconfluently cultivated keratinocytes in fibrin glue (keratinocyte-fibrin-glue-suspension = KFGS), which has successfully been used in burn patients. Altogether 8 patients (average: 57 yrs.) with complex chronic wounds of different origin were treated with KFGS. All wounds, which had been existing from 4 months to 14 years, showed good reepithelization. Up to now there has been observed stable wound closure for 4 years after grafting. This study demonstrates the wound healing potency of a keratinocyte-fibrin-glue-suspension also for chronic wounds. Fibrin glue seems to be both an ideal application vehicle as a biological matrix for the cultivated keratinocytes. The transplantation of cultivated autologous keratinocytes as suspension in fibrin glue is a promising way in the treatment of chronic wounds.
Abteilung Plastische und Handchirurgie, Chirurgische Universitätsklinik Freiburg. bannasch@ch11.ukl.uni-freiburg.de
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D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002478:Cells, Cultured; D002908:Chronic Disease; D003646:Debridement; D005260:Female; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage*; D005500:Follow-Up Studies; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000637:transplantation*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D013997:Time Factors; D014182:Transplantation, Autologous; D014945:Wound Healing; D014947:Wounds and Injuries / Q000601:surgery*
D015718:Fibrin Tissue Adhesive