Adsorption of microorganism components by lixelle beads.
We reported previously that Lixelle, which was used for beta-2 microglobulin (BMG) adsorption columns, could adsorb not only BMG but also inflammatory cytokines. We then were interested in the application of Lixelle to patients with systemic inflammatory response syndrome (SIRS) and tried to find out its ability to adsorb microorganism components in vitro using lipopolysaccharide (LPS) (E. coli: B8), endotoxin (ET) containing water, and peptidoglycan (PG: Micrococcus luteus). The initial concentrations of each solution were LPS (ET: 29,135 EU/L), contaminated water (ET: 3,523 EU/L), and PG (67.1 ng/ml) and 2.5 ml of each of the stock solutions and adjusted diluted solutions contained 0.5 ml of Lixelle beads. After shaking at 37 degrees C for 2 h, ET in the solutions was determined by the ET specific-limulus amebocyte lysate (ES-LAL) method and PG by the silkworm larbae plasma (SLP) method. The results revealed that even when ET concentrations in LPS and contaminated water were high, the samples containing Lixelle beads showed significant decreases. There was some adsorption of PG but no significant differences. Thus, Lixelle beads can adsorb not only BMG but also microorganism components such as ET and PG. These findings, together with the ability to adsorb inflammatory cytokines by Lixelle, show the possibility of application for the treatment of infectious SIRS.
Division of Urology and Artificial Kidney, Osaka City University Hospital, Japan.
K
K
Tsuchida
Y
Y
Takemoto
T
T
Nakatani
T
T
Nakamura
K
K
Sugimura
K
K
Yamamoto
D000327:Adsorption; D004731:Endotoxins / Q000097:blood*; D006464:Hemoperfusion; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000097:blood; D010457:Peptidoglycan / Q000097:blood*; D018709:Statistics, Nonparametric; D018746:Systemic Inflammatory Response Syndrome / Q000378:metabolism*
D004731:Endotoxins; D008070:Lipopolysaccharides; D010457:Peptidoglycan