Photoelectric measurements of s-BLM/nucleoli: a new technique for studying apoptosis.
A new method based on photoelectric measurement for analyzing apoptosis of cell-free MCF-7 nucleoli is reported. Supported bilayer lipid membrane (s-BLM) was used to enclose nucleoli in biological environment. The s-BLM was self-assembled on the wall of a super-thin cell. During the apoptosis induced by Taxol, the photoelectric current of the self-assembled s-BLM/nucleoli was found decreasing with time, suggesting the degradation of nucleus DNA. Electron transfer along the DNA double helix and along nuclear skeleton is assumed in the interpretation. This novel photoelectric analytical method may provide a rapid and sensitive technique to evaluate apoptosis.
Department of Chemistry, Tsinghua University, Beijing, People's Republic of China. fengj@sam.chem.tsinghua.edu.cn
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Feng
C Y
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Zhang
A L
AL
Ottova
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HT
Tien
D017209:Apoptosis / Q000187:drug effects; D002466:Cell Nucleolus / Q000737:chemistry / Q000187:drug effects*; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry*; D017239:Paclitaxel / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D008051:Lipid Bilayers; D017239:Paclitaxel