Increased exon 5 expression alters extrasynaptic NMDA receptors in cerebellar neurons.
We investigated the ontogenic changes in expression of alternatively spliced forms of NR1 protein that contain the N1 cassette (exon 5) in comparison with the total population of NR1 within the rat cerebellum. The N1 cassette is strongly developmentally regulated in the cerebellum, with >80% of total NR1 protein in the adult rat containing the N1 cassette. In contrast, early in development, <20% of NR1 protein contained this cassette. Rat cortices from the same ages did not show an increase in the percent of NR1 protein expressing the N1 cassette, indicating that the developmental changes in the cerebellum are tissue-specific. As the N1 cassette is known to determine NMDA receptor properties, including spermine sensitivity and decay kinetics of glutamate-induced currents, changes in the characteristics of glutamate-activated currents in granule cells from cerebellar slices were compared at postnatal day 7 versus 14. Glutamate responses exhibited fast deactivation kinetics and reduced potentiation by spermine at day 14 while maintaining sensitivity to an NR2B-selective antagonist. These data are consistent with the possibility that N1 cassette expression may be a factor in the developmental changes in properties of extrasynaptic NMDA receptors in the cerebellum.
Department of Pharmacology, Georgetown University School of Medicine, Washington, DC 20007, USA.
K
K
Prybylowski
G
G
Rumbaugh
B B
BB
Wolfe
S
S
Vicini
D000375:Aging; D017398:Alternative Splicing; D000818:Animals; D000831:Animals, Newborn; D002462:Cell Membrane / Q000502:physiology; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D002540:Cerebral Cortex / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D066298:In Vitro Techniques; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D013096:Spermine / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology
C409466:NR1 NMDA receptor; D016194:Receptors, N-Methyl-D-Aspartate; D013096:Spermine