In vivo detection of vascular adhesion protein-1 in experimental inflammation.
Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.
Jaakkola
K
K
National Public Health Institute and MediCity Research Laboratory, the Department of Medicine, Nuclear Medicine, and Pharmacology and Clinical Pharmacology, the University of Turku, Turku, Finland.
Nikula
T
T
Holopainen
R
R
Vähäsilta
T
T
Matikainen
M T
MT
Laukkanen
M L
ML
Huupponen
R
R
Halkola
L
L
Nieminen
L
L
Hiltunen
J
J
Parviainen
S
S
Clark
M R
MR
Knuuti
J
J
Savunen
T
T
Kääpä
P
P
Voipio-Pulkki
L M
LM
Jalkanen
S
S
D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D004285:Dogs; D015902:Gamma Cameras; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D007457:Iodine Radioisotopes; D051379:Mice; D011877:Radionuclide Imaging; D012867:Skin / Q000737:chemistry / Q000000981:diagnostic imaging / Q000473:pathology; D013552:Swine; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007457:Iodine Radioisotopes; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing)