Topical acetyl salicylate and dipyrone attenuate neurogenic protein extravasation in rat skin in vivo.
The effect of topically applied acetyl salicylic acid (ASA) and dipyrone on capsaicin-evoked protein extravasation was investigated by dermal microdialysis in rat. After a baseline of 75 min, capsaicin (1%) was applied epicutaneously under occlusion for 75 min above the capillaries. Topical capsaicin stimulation induced neurogenic protein extravasation with a mean increase of protein concentration in the perfusate of 165+/-27% (mean+/-SEM; n=15), whereas in sham-stimulated sites protein concentration decreased to 73+/-7% of the prestimulation value (n=6). ASA (2-200 mg/ml) and dipyrone (3-300 mg/ml) dose-dependently reduced the capsaicin induced protein extravasation to 118+/-23% (ASA, 200 mg/ml; n=8) and 72+/-9% (dipyrone, 300 mg/ml; n=8) of the prestimulation value. ASA and dipyrone antagonized the excitatory effects of capsaicin on skin nociceptors and thus suppressed the neurogenic protein extravasation.
Schmelz
M
M
Department of Physiology and Experimental Pathophysiology, Universitätsstrasse 17, 91054 Erlangen-, Nürnberg, Germany. schmelz@physiologie1.uni-erlangen.de
Weber
S
S
Kress
M
M
D000287:Administration, Topical; D000636:Aminosalicylic Acids / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D002211:Capsaicin / Q000008:administration & dosage / Q000037:antagonists & inhibitors; D004177:Dipyrone / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D020078:Neurogenic Inflammation / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism*
D000636:Aminosalicylic Acids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004177:Dipyrone; C028836:N-acetyl-5-aminosalicylic acid; D002211:Capsaicin