A de novo philadelphia chromosome-positive acute mixed-lineage leukemia with both major and minor BCR/ABL mRNA transcripts.
A patient with a Philadelphia chromosome (Ph)-positive acute mixed-lineage leukemia (AMLL) expressing both major and minor BCR/ABL mRNA transcripts is described. Phenotypic analysis of the leukemic blasts revealed positivity for both myeloid and B-cell lineages. Southern blot analysis showed a rearrangement of the immunoglobulin heavy chain (IgH) gene. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed the expression of both major and minor BCR/ABL mRNA transcripts. To our knowledge, this is the first report of AMLL expressing both major and minor BCR/ABL mRNA transcripts and rearrangement of the IgH gene.
Department of Hematology, Jichi Medical School, Minamikawachi-machi, Tochigi-ken, Japan.
T
T
Tarumoto
S
S
Imagawa
K
K
Ohmine
A
A
Mano
T
T
Nagai
M
M
Takatoku
K
K
Muroi
K
K
Hatake
K
K
Ozawa
D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000008:administration & dosage; D015139:Blotting, Southern; D001854:Bone Marrow Cells / Q000473:pathology; D002051:Burkitt Lymphoma / Q000188:drug therapy / Q000235:genetics*; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D015321:Gene Rearrangement; D006651:Histocytochemistry; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000235:genetics*; D008297:Male; D009195:Peroxidase / Q000032:analysis; D010677:Philadelphia Chromosome; D012333:RNA, Messenger / Q000032:analysis*; D012074:Remission Induction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic
D007143:Immunoglobulin Heavy Chains; D012333:RNA, Messenger; D009195:Peroxidase; D016044:Fusion Proteins, bcr-abl