Chloride fluxes during cAMP stimulation of liquid absorption across the native rat alveolar epithelium.
cAMP activates Cl- channels in the alveolar epithelium of rabbits (Nielsen et al. Am J Physiol. 1998; 275:L1127-L1133), resulting in Cl- secretion; and of rats (Jiang et al. Am J Physiol. 1998; 275:C1610-C1620), resulting in Cl- absorption. The relationship between Cl- fluxes and liquid absorption across the alveolar barrier was examined using isolated perfused rat lungs. Unidirectional 36Cl- fluxes (apparent PACl products) in the apical-to-basal (absorption, PAClab) and reverse (secretion, PAClba) directions were identical under control conditions. Both increased about 2-fold during stimulation of liquid absorption by dibutyrylcAMP + isobutylmethylxanthine (P < .001). Inhibiting Na+ and liquid absorption during cAMP stimulation by adding amiloride to the alveolar instillate decreased PAClab to control level (P < .01), but did not affect PAClba. Neither alveolar liquid absorption nor PAClba was affected by apical N-phenylanthranilic acid or basolateral bumetanide during cAMP stimulation. Mannitol permeability--surface area products did not differ with the experimental condition. These observations indicate that cAMP stimulation results in enhanced Cl- and liquid absorption from rat airspaces and Cl- secretion into them. They suggest that Cl- absorption follows Na+ transport, but fail to demonstrate any significant participation of cAMP-activated Cl- channels in these changes.
INSERM U82, Faculté Xavier Bichat, Paris, France. saumon@bichat.inserm.fr
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D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002034:Bumetanide / Q000494:pharmacology; D018118:Chloride Channels / Q000187:drug effects / Q000502:physiology; D002712:Chlorides / Q000378:metabolism* / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology*; D004232:Diuretics / Q000494:pharmacology; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D005110:Extracellular Space / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008353:Mannitol / Q000494:pharmacology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D018118:Chloride Channels; D002712:Chlorides; D004232:Diuretics; D010726:Phosphodiesterase Inhibitors; D002034:Bumetanide; D008353:Mannitol; D000584:Amiloride; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine