[Effects of erythropoietin or nitric oxide synthesis inhibitor on hyperdynamic circulatory state in cirrhotic rats].
OBJECTIVE
To observe the effects of erythropoietin or nitric oxide synthesis (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on hyperdynamic circulatory state in rats with cirrhosis.


METHODS
Cirrhotic rat model was made. Cirrhotic rats were treated with NOS inhibitor L-NAME (0.5 mg.kg-1.d-1) by gavage or with erythropoietin (100 U/kg) injected subcutaneously for two weeks. The hemodynamic parameters in cirrhotic rats treated with L-NAME or with erythropoietin were determined by using 57Co-labled microsphere technique. Serum nitric oxide (NO) levels were also measured by using a fluorometric assay.


RESULTS
Hyperdynamic circulatory state was observed in all rats with cirrhosis. Serum NO levels in cirrhotic rats were significantly higher than that in normal controls. Hyperdynamic circulation status in cirrhotic rats treated with erythropoietin or with L-NAME was markedly attenuated. As compared with untreated-cirrhotic rats, serum NO concentration in erythropoietin-treated and L-NAME-treated cirrhotic rats were significantly lower.


CONCLUSIONS
L-NAME treatment could reverse the hyperdynamic circulatory state in cirrhotic rats which might be ameliorated by inactivation of overproduced NO by increasing hemoglobin with erythropoietin.
Shanghai Institute of Digestive Disease, Ren Ji Hospital, Shanghai Second Medical University.
Y
Y
Huang
S
S
Xiao
D
D
Zhang
D000818:Animals; D004921:Erythropoietin / Q000494:pharmacology*; D006439:Hemodynamics / Q000187:drug effects*; D008103:Liver Cirrhosis / Q000503:physiopathology*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052250:Nitric Oxide Synthase Type III; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D004921:Erythropoietin; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D019331:NG-Nitroarginine Methyl Ester