N-ethylmaleimide-sensitive factor is associated with the nuclear envelope.
N-Ethylmaleimide-sensitive factor (NSF) is an ATPase involved in many membrane fusion events within the exocytic and endocytotic pathways. In the present study we showed that NSF is associated with the nuclear envelope. Golgi-associated NSF was released from membranes upon incubation with Mg(2+)-ATP, reflecting the disassembly of a complex consisting of NSF, soluble NSF attachment proteins (SNAPs), and SNAP receptors (SNAREs). In contrast nuclear envelope-associated NSF in interphase cells was not released by the same treatment. During mitosis, however, it was released from nuclear membranes by Mg(2+)-ATP. These results suggest that the binding mode of nuclear membrane-associated NSF changes during the cell cycle.
School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
J
J
Mashima
M
M
Nagahama
K
K
Hatsuzawa
K
K
Tani
T
T
Horigome
A
A
Yamamoto
M
M
Tagaya
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D002453:Cell Cycle; D002455:Cell Division; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D015151:Immunoblotting; D007399:Interphase; D034882:Lamins; D008361:Mannosidases / Q000032:analysis; D008938:Mitosis / Q000187:drug effects; D052066:N-Ethylmaleimide-Sensitive Proteins; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D034882:Lamins; D009687:Nuclear Proteins; D033921:Vesicular Transport Proteins; D000255:Adenosine Triphosphate; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D052066:N-Ethylmaleimide-Sensitive Proteins; C498737:Nsf protein, rat