[Application of gas chromatography in the identification of Enterobacter cloacae, Enterobacter aerogenes, and Enterobacter agglomerans].
Enterobacter cloacae, Enterobacter aerogenes and Enterobacter agglomerans were identified using gas chromatography as a substitution of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 120 samples were analyzed from reference strains (ATCC 13047, 13048, 27155) and environmental isolations, eleven fatty acids were included in the profiles from which cis-9, 10-methyleneoctadecanoic acid (peak 24), cis-9-hexadecenoic acid (peak 14), octadecanoic acid (peak 23) and dodecanoic acid (peak 3), were the most important for the differentiation of the three species analyzed.
Robles Valderrama
E
E
Unidad de Investigación Interdisciplinaria en Ciencias d e la Salud y la Educación, UNAM Campus Iztacala, México. erobles@servidor.unam.mx
Ramírez García
P
P
González Arreaga
M E
ME
Sáinz Morales
M G
MG
Martínez Rodríguez
B
B
Durán Díaz
A
A
Chávez Ramírez
D
D
D001431:Bacteriological Techniques; D015415:Biomarkers; D002849:Chromatography, Gas; D004754:Enterobacter / Q000145:classification / Q000302:isolation & purification*; D016972:Enterobacter cloacae / Q000302:isolation & purification; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D019308:Palmitic Acid / Q000032:analysis; D013045:Species Specificity; D013229:Stearic Acids / Q000032:analysis
D015415:Biomarkers; D004952:Esters; D005227:Fatty Acids; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid; C029355:dihydrosterculic acid