Utility of commercial systems for identification of Burkholderia cepacia complex from cystic fibrosis sputum culture.
Performances of several commercial test systems were reviewed to determine their relative levels of accuracy in identifying Burkholderia cepacia complex isolates recovered from cystic fibrosis sputum culture. Positive predictive values ranged from 71 to 98%; negative predictive values ranged from 50 to 82%. All systems misidentified B. cepacia complex. The species most frequently misidentified as B. cepacia was Burkholderia gladioli. These data support the results of previous studies that recommend confirmatory testing, including the use of DNA-based methods, for sputum culture isolates presumptively identified as B. cepacia.
Shelly
D B
DB
Clinical Microbiology Laboratory, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA.
Spilker
T
T
Gracely
E J
EJ
Coenye
T
T
Vandamme
P
P
LiPuma
J J
JJ
D019121:Burkholderia Infections / Q000150:complications / Q000382:microbiology*; D016956:Burkholderia cepacia / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D003470:Culture Media; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D006801:Humans; D007753:Laboratories; D008829:Microbiology; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D013183:Sputum / Q000382:microbiology*
D003470:Culture Media; D011933:Reagent Kits, Diagnostic