Monoclonal antibodies to Rh D--development and uses.
Monoclonal anti-D has proved impossible to make in rodent systems. Human monoclonal anti-D has been produced using EBV transformed peripheral B cells, coupled with fusions to myeloma cell lines. More recently molecular biology techniques have been used to produce monoclonal anti-D. The range of monoclonal anti-D produced is considered. The selection of monoclonal anti-D for use as blood grouping reagents for typing donors and recipients is reviewed--all types of D positive should be typed as positive when donors are considered. However, DVI patients should be typed as D negative. Considerations for the development of monoclonal anti-D for prophylactic use are reviewed.
Bristol Institute for Transfusion Sciences, UK. marion.scott@nbs.nhs.uk
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes / Q000097:blood; D007074:Immunoglobulin G / Q000627:therapeutic use; D007159:Immunologic Tests; D008297:Male; D012203:Rh Isoimmunization / Q000517:prevention & control; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D012204:Rh-Hr Blood-Group System