Inducible nitric oxide synthase mRNA is upregulated in skin tumors of v-ha-ras transgenic TG-AC mice treated with 12-o-tetradecanoylphorbol-13-acetate.
The correlation between the steady-state level of inducible nitric oxide synthase (iNOS) mRNA and skin tumors induced following treatment with 12-o-tetradecanoylphorbol-13-acetate (TPA) was investigated in transgenic TG-AC mice, which carry the v-Ha-ras oncogene fused to the promoter of the mouse embryonic alpha-like, zeta-globin gene. In animals treated with TPA (2.5 microg x 2/week, for 2 weeks), the increase of iNOS mRNA was locally confined only to the regions of papillomas, but not to the skin tissues surrounding the papillomas. However, the tissues surrounding the papillomas showed only a minor increase in the steady-state level of iNOS mRNA. These data suggest that iNOS gene expressions may underlie tumorigenesis during TPA promotion in TG-AC mice.
Division of' Toxicology, College of Pharmacy, SungKyunKwan University, Suwon, Kyunggi-do, South Korea. bmlee@skku.ac.kr
B M
BM
Lee
H S
HS
Kim
G H
GH
Jeohn
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D051379:Mice; D008822:Mice, Transgenic; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D015854:Up-Regulation
D002273:Carcinogens; D012333:RNA, Messenger; D001564:Benzo(a)pyrene; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D013755:Tetradecanoylphorbol Acetate