Analysis of tnrA alleles which result in a glucose-resistant sporulation phenotype in Bacillus subtilis.
Bacillus subtilis cells cannot sporulate in the presence of catabolites such as glucose. During the analysis of Tn10-generated mutants, we found that deletion of the C-terminal region of the tnrA gene, which encodes a global regulator that positively regulates a number of genes in response to nitrogen limitation, results in a catabolite-resistant sporulation phenotype. Analyses of nrg-lacZ and nasB-lacZ, which are activated by TnrA under nitrogen limitation, showed that C-terminally truncated TnrA activates nitrogen-regulated genes constitutively. The relief of catabolite repression of sporulation may result from the uncontrolled expression of the TnrA-regulated genes.
Laboratory of Microbial and Bioprocess Engineering, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea 305-600.
B S
BS
Shin
S K
SK
Choi
I
I
Smith
S H
SH
Park
D000483:Alleles; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005947:Glucose / Q000494:pharmacology*; D016296:Mutagenesis; D009584:Nitrogen / Q000378:metabolism; D010641:Phenotype; D012097:Repressor Proteins; D013171:Spores, Bacterial; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D019895:Transposases
D001426:Bacterial Proteins; D003470:Culture Media; D012097:Repressor Proteins; C101458:ScgR protein, Bacillus subtilis; D014157:Transcription Factors; D019895:Transposases; D005947:Glucose; D009584:Nitrogen