Kinetic characterisation of ene-diol-based inhibitors of alpha-amylase.
A kinetic analysis of the inhibition of malt alpha-amylase by compounds based on ascorbic acid has shown the mode of inhibition to be competitive for the parent compound, but more complex for its derivatives. We have further simplified the ascorbic acid ene-diol pharmacophore by demonstrating that dihydroxyfumaric acid is also a good inhibitor of malt alpha-amylase.
Gerrard
J A
JA
Department of Plant and Microbial Sciences, University of Canterbury, Christchurch, New Zealand. a.abell@chem.canterbury.ac.nz
Prince
M J
MJ
Abell
A D
AD
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002523:Edible Grain / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D007700:Kinetics; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D000516:alpha-Amylases; D001205:Ascorbic Acid