[Effect of ganglioside GM1 on the metabolism of Alzheimer amyloid beta-protein precursor].
OBJECTIVE
To study the effect of GM1 ganglioside interfering the metabolism of amyloid beta-protein precursor (APP) on cells for exploring the relationship between GM1 and development of Alzheimer senile plaques and the other amyloidoses.


METHODS
GM1 influencing the metabolism of APP and the release of its metabolite secretory APP on human APP695 cDNA transfected cells were detected by metabolic radiolabeling. The affinity of GM1 and recombinant secretory APP was analyzed by Western blot and dot blot.


RESULTS
GM1 specifically bound APP, and interfered the proteolysis of APP and inhibited the release of secretory APP on cells.


CONCLUSIONS
The abnormal metabolism of GM1 may contribute to the aggregation and deposition of amyloid beta-protein in Alzheimer brains.
Institute of Mental Health, Beijing Medical University.
D
D
Zhang
Y
Y
Ruan
K
K
Beyreuther
D000544:Alzheimer Disease / Q000209:etiology / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D006801:Humans
D016564:Amyloid beta-Protein Precursor; D005677:G(M1) Ganglioside