[Position effect variegation of the mosaic type, arising as a result of transposition AR4-24P[white, rosy] in the Drosophila melanogaster genome].
A line with the mosaic expression of the white+ transgene was obtained by inducing transposition of the AR4-24P[white, rosy] transposon and was used for the second round of induction. As a result, 57 lines with the mosaic eye pigmentation were obtained. In situ hybridization and Southern blotting showed that genomic DNA fragments flanking AR4-24 were, in some cases, transposed together with the transposon. A spontaneous loss of these fragments resulted in reversion to the wild-type phenotype. The mosaic eye pigmentation in a line that carried the AR4-24 transposon flanked with the same fragments in region 24D1-2 was not affected by the Su(var)3-6 gene modifying position effect variegation (PEV). Other PEV modifiers, Su(var)3-9 and Su(var)2-5, had only a slight effect on PEV; Su(var)3-7 restored the wild-type phenotype. The genomic fragments captured by the transposon may contain DNA sequences that autonomously induce mosaic PEV of the white gene.
Balasov
M L
ML
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia.
Beliaeva
E S
ES
Shestopal
S A
SA
Makunin
I V
IV
Zhimulev
I F
IF
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D016678:Genome; D017403:In Situ Hybridization; D009030:Mosaicism; D012168:Retinal Pigments / Q000235:genetics; D019076:Transgenes
D004251:DNA Transposable Elements; D012168:Retinal Pigments; D004247:DNA