Dopaminergic phenotype of hNT cells in vitro.
We investigated the catecholaminergic nature of cultured hNT neurons previously treated either for 4 or 5 weeks with retinoic acid (RA). There were significantly more tyrosine hydroxylase (TH)-positive neurons (60%) in cultures treated for 4 weeks with RA compared to 5 week-treated cultures (</=15%). Furthermore, numerous TH-positive hNT cells were also immunoreactive to dopamine transporter (DAT), dopamine receptor (D2) and aldehyde dehydrogenase (AHD-2), an enzyme exclusively expressed by dopaminergic (DA) ventral mesencephalic (VM) precursors. Thus this cell line has all the necessary cellular machinery to produce functional DA neurons and therefore is a good alternative tissue source to fetal VM.
Department of Neurosurgery, College of Medicine, University of South Florida, Tampa, FL 33612, USA. tzigova@com1.med.usf.edu
T
T
Zigova
L F
LF
Barroso
A E
AE
Willing
S
S
Saporta
M P
MP
McGrogan
T B
TB
Freeman
P R
PR
Sanberg
D000444:Aldehyde Dehydrogenase / Q000032:analysis; D000970:Antineoplastic Agents / Q000494:pharmacology; D002352:Carrier Proteins / Q000032:analysis; D002460:Cell Line; D004298:Dopamine / Q000032:analysis* / Q000235:genetics*; D050483:Dopamine Plasma Membrane Transport Proteins; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008636:Mesencephalon / Q000166:cytology; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D010641:Phenotype; D017448:Receptors, Dopamine D2 / Q000032:analysis; D014212:Tretinoin / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D000970:Antineoplastic Agents; D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D017448:Receptors, Dopamine D2; D014212:Tretinoin; D014446:Tyrosine 3-Monooxygenase; D000444:Aldehyde Dehydrogenase; D004298:Dopamine