Remnant lipoproteins induce proatherothrombogenic molecules in endothelial cells through a redox-sensitive mechanism.
BACKGROUND
Triglyceride-rich lipoproteins (TGLs) are atherogenic. However, their cellular mechanisms remain largely unexplained. This study examined the effects of isolated remnant-like lipoprotein particles (RLPs) on the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and tissue factor (TF), proatherothrombogenic molecules, in cultured human endothelial cells.


RESULTS
RLPs were isolated from plasma of hypertriglyceridemic patients by use of the immunoaffinity gel mixture of anti-apoA-1 and anti-apoB-100 monoclonal antibodies. The incubation of cells with RLPs significantly upregulated mRNA and protein expression of these molecules. Total TGLs (d<1.006) and LDL had fewer or minimal effects on expression of these molecules compared with RLPs. RLPs increased intracellular oxidant levels, as assessed with an oxidant-sensitive probe. Combined incubation with alpha-tocopherol or N-acetylcysteine, both antioxidants, suppressed RLP-induced increase in expression of these molecules. In patients with higher plasma levels of RLPs, plasma levels of soluble forms of ICAM-1 and VCAM-1 were significantly higher than in patients with lower RLP levels. Treatment with alpha-tocopherol for 1 month decreased levels of the soluble adhesion molecules concomitantly with an increase in resistance of RLPs to oxidative modification in patients with high RLP levels.


CONCLUSIONS
RLPs upregulated endothelial expression of ICAM-1, VCAM-1, and TF, proatherothrombogenic molecules, partly through a redox-sensitive mechanism. RLPs may have an important role in atherothrombotic complications in hypertriglyceridemic patients.
Doi
H
H
Department of Cardiovascular Medicine, Kumamoto University School of Medicine, Kumamoto City, Japan.
Kugiyama
K
K
Oka
H
H
Sugiyama
S
S
Ogata
N
N
Koide
S I
SI
Nakamura
S I
SI
Yasue
H
H
D001161:Arteriosclerosis / Q000188:drug therapy / Q000209:etiology*; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000097:blood / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012995:Solubility; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood / Q000235:genetics / Q000378:metabolism; D014810:Vitamin E / Q000627:therapeutic use
D003470:Culture Media; D008054:Lipid Peroxides; D008074:Lipoproteins; D016328:NF-kappa B; D010446:Peptide Fragments; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D014810:Vitamin E; D004247:DNA; D013925:Thromboplastin