[Molecular characterization of rotavirus in the city of Santafé de Bogotá, Colombia. Determination of the electrophenotypes and typing of a strain by RT-PCR].
131 diarrheic samples obtained from children taken to the emergency room of the Misericordia Hospital in Santafé de Bogotá were cultivated for rotavirus. These virus were found to be etiological agents for the diarrhea in 42 (32%) of the patients. In order to characterize electrophoretically the rotavirus they were cultured in cell line MA-104, total RNA was extracted and subjected to electrophoresis in SDS-PAGE and then the retrotranscription polymerase chain reaction. Eight different electrophoretic patterns were found. All patterns correspond to group A. Eight positive PAGE samples and the control type RRV were cultured in cell line MA-104. Viral growth was found only in the control and four of the samples. Total RNA was extracted from all five infected cultures and gene 9, which codifies for protein VP7 was amplified. All four samples and the control were shown to be G3. The finding of eight different electrophoretic types, all of them belonging to group A, show a diversity in co-circulating rotavirus strains. These findings contribute to a better understanding of viral acute gastroenteritis natural history and should help in developing new epidemiological vigilance strategies, prevention and control of rotavirus in children population of Santafé de Bogotá.
Pontificia Universidad Javeriana.
A
A
Correa
Y
Y
Solarte
J
J
Barrera
D
D
Mogollón
M F
MF
Gutiérrez
D002460:Cell Line; D002675:Child, Preschool; D003105:Colombia / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006801:Humans; D007223:Infant; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D014505:Urban Population; D014776:Virus Cultivation
D012367:RNA, Viral