Gene transfer into chicken embryos by retrovirus vectors.
While chickens have many properties that are advantageous for embryological studies, their genetic analysis has been restricted. However, by using retrovirus vector systems in combination with classical techniques of experimental developmental biology, it has recently become possible to analyze the function of genes involved in the development of this organism. Avian retrovirus vectors are unique in that they can be divided into two categories: replication-competent and replication-defective (replication-incompetent). By choosing the vectors correctly, there are many experimental applications of these vectors such as induction of constitutive (or regulated) gene expression in a restricted region of tissues, organs and embryos; cell lineage analysis; and formation of concentration gradients of morphogens in micromass cultures. In this paper, several retrovirus vectors available for the chicken will be introduced and their applications in developmental biology will be reviewed.
Department of Gene Regulation, Institute of Medical Science, University of Tokyo, Japan. iba@ims.u-tokyo.ac.jp
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D000818:Animals; D019070:Cell Lineage; D002642:Chick Embryo; D004279:DNA, Viral / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D012190:Retroviridae / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral