Of mice and men: hybridoma and recombinant antibodies.
Thousands of mouse monoclonal antibodies have been produced from hybridomas over the past 25 years. The same technique can now be used to clone human antibodies from transgenic mice. Full-length antibodies and recombinant fragments engineered for various diagnostic and therapeutic applications can be obtained in reasonably large amounts after expression in mammalian cells, milk and plants.
Little
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Affirmed Therapeutics AG, Dr-Albert-Reimann-Strabetae 2, 68526 Ladenburg, Germany.
Kipriyanov
S M
SM
Le Gall
F
F
Moldenhauer
G
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001419:Bacteria; D001709:Biotechnology / Q000379:methods*; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007128:Immunoglobulin Fragments / Q000096:biosynthesis; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000378:metabolism; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000096:biosynthesis; D014170:Transformation, Genetic
D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D011994:Recombinant Proteins