High performance liquid chromatographic determination of ambroxol in the presence of different preservatives in pharmaceutical formulations.
A high-performance chromatographic method is described for simultaneous determination of ambroxol in the presence of different preservatives in syrups. The method separates ambroxol from methyl- ethyl-, propyl- and butyl paraben and from other multi-component mixtures. The retention behaviour of ambroxol and parabens as a function of both pH and mobile phase composition was investigated. The eluents were monitored with a UV detector at 247 nm. Linear relationships between the amount of pharmaceutical compounds and peak heights were confirmed at the concentrations of 0.74-14.08 microg ml(-1). The high recovery (no extraction of the samples is required) and the low %RSD confirm the suitability of the proposed method for the determination of ambroxol in different pharmaceutical preparations.
Laboratory of Pharmaceutical Analysis, School of Pharmacy, Aristotelian University, Thessaloniki, Greece. koundour@pharm.auth.gr
J E
JE
Koundourellis
E T
ET
Malliou
T A
TA
Broussali
D000551:Ambroxol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005100:Expectorants / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D005100:Expectorants; D004364:Pharmaceutical Preparations; D000551:Ambroxol