Microfilaments in regenerating cells of rabbit cornea: immunological and ultrastructural observations.
The role of myosin-like protein in regenerating and proliferating corneal cells following a standard alkali-injury in the rabbit eye has been studied. Microfilaments were observed by conventional transmission electron microscopy (T.E.M.) in injured epithelium and in fibroblasts. Typical microfilament bundles with electron dense structures and with stress fibers were evident. The presence of myosin-like proteins was demonstrated by means of immunochemical and autoradiographical techniques. Both epithelial cells and fibroblasts bind antimyosin-like antibodies (AMA). The same cells studied with the E.M. showed bundles of microfilaments in the cortical areas of their cytoplasm in correspondence with the same side of fluorescent or labelled AMA. The immunochemical and ultrastructural results suggest that both cells are able to produce in vivo movements involved in morphogenetic phenomena. Therefore, these structures play a role in post-traumatic corneal regeneration.
Laboratories of Neurophysiopathology, II Neurologic Clinic, Pecs, Hungary.
D
D
Cavallotti
M
M
Artico
J
J
Feher
N
N
Pescosolido
S
S
De Santis
C
C
Cavallotti
D008841:Actin Cytoskeleton / Q000502:physiology*; D000818:Animals; D001345:Autoradiography; D003315:Cornea / Q000502:physiology* / Q000648:ultrastructure; D007120:Immunochemistry; D008854:Microscopy, Electron; D011817:Rabbits / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*