mRNA expression of kidney-specific ClC-K1 chloride channel in single-cell reverse transcription-polymerase chain reaction analysis of outer hair cells of rat cochlea.
Outer hair cells (OHCs) of cochlea have been suggested to have Cl(-) channels sensitive to an ototoxic diuretic, furosemide. We therefore examined whether kidney-specific chloride channels (ClC-K1 and ClC-K2) and ClC-5 are also expressed in OHCs of rat cochlea, assuming that these channels might be the targets of oto-nephrotoxic drugs, by single-cell reverse transcription-polymerase chain reaction (RT-PCR) technique. Single-cell RT-PCR revealed the presence of transcripts of ClC-K1 in OHCs which was verified by DNA sequencing, while ClC-K2 and ClC-5 were not detected. The possible roles of ClC-K1 in OHCs are discussed.
Kawasaki
E
E
Department of Pharmacology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi-shi, 570-8506, Osaka, Japan.
Hattori
N
N
Miyamoto
E
E
Yamashita
T
T
Inagaki
C
C
D000818:Animals; D018118:Chloride Channels / Q000096:biosynthesis* / Q000235:genetics; D003051:Cochlea / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D009865:Oocytes; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014981:Xenopus; D029867:Xenopus Proteins
C097503:CLC-5 chloride channel; D018118:Chloride Channels; C489933:Clcnka protein, mouse; C082118:Clcnka protein, rat; D012333:RNA, Messenger; D029867:Xenopus Proteins; C489934:clcnka protein, Xenopus