[Loss of heterozygosity on chromosome 17p13.3 in ovarian cancer and cervical cancer].
OBJECTIVE
To identify the loss of heterozygosity (LOH) on chromosome 17p13.3 in ovarian cancer and cervical cancer.


METHODS
The frequency of LOH on chromosome 17p13.3 in DNA samples from 24 ovarian cancers, 9 cervical cancers, and 13 non-malignant gynecological diseases were determined respectively, using Southern blot method with probe PYNZ. 22.


RESULTS
LOH on 17p13.3 was found in 12 of 24 (50.0%) ovarian cancers (including a borderline mucinous cystadenoma), 4 of 9 (44.4%) cervical carcinomas, and 1 of 13 (7.7%) non-malignant gynecological diseases, which was cervical intraepithelial neoplasm III (CIN III) (P < 0.01).


CONCLUSIONS
These results show that LOH on 17p13.3 is associated with ovarian cancer and cervical cancer, suggesting that detection of LOH on 17p13.3 may be helpful to understand the molecular pathogenesis of ovarian cancer and cervical cancer.
Department of Gynecology, Shanghai Cancer Hospital, Shanghai Medical University.
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Zhang
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Yang
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Xu
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002886:Chromosomes, Human, Pair 17; D003536:Cystadenocarcinoma / Q000235:genetics; D018282:Cystadenocarcinoma, Mucinous / Q000235:genetics; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000235:genetics*; D002578:Uterine Cervical Dysplasia / Q000235:genetics