Novel insertion sequence elements associated with genetic heterogeneity and phenotype conversion in Ralstonia solanacearum.
Three insertion sequences (IS) elements were isolated from the phytopathogen Ralstonia solanacearum. Southern hybridization using these IS elements as probes revealed hybridization profiles that varied greatly between different strains of the pathogen. During a spontaneous phenotype conversion event, the promoter of the phcA gene was interrupted by one of these IS elements.
Jeong
E L
EL
Department of Genetics, The University of Adelaide, Adelaide, South Australia 5005, Australia. eljeong@genetics.adelaide.edu.au
Timmis
J N
JN
D001426:Bacterial Proteins / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D016946:Gram-Negative Aerobic Rods and Cocci / Q000235:genetics*; D010641:Phenotype; D010944:Plants / Q000382:microbiology; D010957:Plasmids; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C082933:PhcA protein, Ralstonia solanacearum; D014157:Transcription Factors