[Apoptosis and hepatic cell proliferation in autoimmune hepatitis and chronic viral hepatitis C in children: analysis of APO-1/Fas (CD95), bcl-2 and Ki67 expression].
Hepatocyte damage in autoimmune hepatitis (AIH) and chronic viral hepatitis C (CVH) is attributed to an immune response. We analysed liver biopsy specimens from 4 children with AIH type I, 3 children with AIH type II and 2 children with CVH, using ApopDetek in situ hybridisation method and Mabs anti CD95, Ki67, bcl-2 by means of APAAP technique. The histological appearance of apoptotic bodies in both conditions was similar. The proliferation activity of the hepatocytes was elevated in cases of CVH and less extensive in AIH. Immunohistochemical analysis suggested that the liver damage in AIH and CVH could be mediated by CD95 system as a mechanism of T-cell mediated cytotoxicity.
Zakład Patologii IP-CZD, Instytut Pomnik-Centrum Zdrowia Dziecka w Warszawie.
J
J
Cielecka-Kuszyk
W
W
Dura
E
E
Czarnowska
J
J
Pawłowska
M
M
Woźniak
M
M
Woynarowski
D000293:Adolescent; D017209:Apoptosis; D001706:Biopsy; D002455:Cell Division; D002648:Child; D019254:Genes, bcl-2; D019698:Hepatitis C, Chronic / Q000276:immunology* / Q000473:pathology*; D019693:Hepatitis, Autoimmune / Q000276:immunology* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008099:Liver / Q000473:pathology*; D019014:fas Receptor / Q000032:analysis
D019394:Ki-67 Antigen; D019014:fas Receptor