[The use of biological markers in the diagnosis and follow-up of patients with multiple sclerosis. Test of five fluids].
METHODS
We studied five biological fluids which were easily accessible to immunological examination (cerebrospinal fluid, plasma, tears, saliva and urine) in 25 patients with multiple sclerosis, clinically definite according to the criteria of Cleveland, Ohio (1991) and tabulated according to the Kurztke's expanded disability status scale. The samples were obtained simultaneously during a clinical bout of the disease before any pharmacological or immunosuppressive treatment had been given.


RESULTS
The soluble interleukin-2 levels were significantly raised in at least three of these fluids--always absent from the urine--when compared with normal controls. The sensitivity and specificity of this determination for diagnosis of the condition was greater than that of other immunochemical parameters--oligoclonal distribution of immunoglobulins (specifically of IgG), imbalance of the light Kappa and Lambda chains--and physiological studies (evoked potentials). The dosification and quantification of basic myelin protein of the central nervous system, rich in citruline in the urine, may be a parameter of progressiveness.


CONCLUSIONS
This methodology (five humours test) may be used to establish an earlier, more certain diagnosis of multiple sclerosis and also monitor its biological activity together with nuclear magnetic resonance with intravenous contrast.
Oehninger-Gatti
C
C
Sección de Enfermedades Desmielinizantes, Hospital de Clínicas, Montevideo, Uruguay. coehning@mednet.org.uy
Buzó
R
R
Alcántara
J C
JC
Chouza
C
C
Gómez
A
A
Cibils
D
D
Gordon-Firing
S
S
D000328:Adult; D015415:Biomarkers; D003071:Cognition / Q000502:physiology*; D005074:Evoked Potentials, Visual / Q000502:physiology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007376:Interleukin-2 / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D008297:Male; D009103:Multiple Sclerosis / Q000175:diagnosis* / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013666:Tears / Q000737:chemistry*
D015415:Biomarkers; D007376:Interleukin-2; D015375:Receptors, Interleukin-2