Effect of metal ions on the activity of green crab (Scylla serrata) alkaline phosphatase.
Green crab (Scylla serrata) alkaline phosphatase (EC 3.1.3.1) is a metalloenzyme, which catalyzes the nonspecific hydrolysis of phosphate monoesters. The present paper deals with the study of the effect of some kinds of metal ions on the enzyme. The positive monovalent alkali metal ions (Li(+), Na(+) and K(+)) have no effect on the enzyme; positive bivalent alkaline-earth metal ions (Mg(2+), Ca(2+) and Ba(2+)) and transition metal ions (Mn(2+), Co(2+), Ni(2+) and Cd(2+)) activate the enzyme; heavy metal ions (Hg(2+), Ag(+), Bi(2+), Cu(2+) and Zn(2+)) inhibit the enzyme. The activation of magnesium ion on the enzyme appears to be a partial noncompetitive type. The kinetic model has been set up and a new plot to determine the activation constant of Mg(2+) was put forward. From the plot, we can easily determine the activation constant (K(a)) value and the activation ratio of Mg(2+) on the enzyme. The inhibition effects of Cu(2+) and Hg(2+) on the enzyme are of noncompetitive type. The inhibition constants have been determined. The inhibition effect of Hg(2+) is stronger than that of Cu(2+).
Chen
Q X
QX
Department of Biology, Xiamen University, 361005, Xiamen, People's Republic of China.
Zheng
W Z
WZ
Lin
J Y
JY
Shi
Y
Y
Xie
W Z
WZ
Zhou
H M
HM
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D003386:Brachyura / Q000201:enzymology*; D002413:Cations, Divalent / Q000494:pharmacology; D002414:Cations, Monovalent / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007700:Kinetics; D008667:Metalloproteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D008670:Metals / Q000494:pharmacology*; D008673:Metals, Alkaline Earth / Q000494:pharmacology; D019216:Metals, Heavy / Q000494:pharmacology
D002413:Cations, Divalent; D002414:Cations, Monovalent; D004791:Enzyme Inhibitors; D008667:Metalloproteins; D008670:Metals; D008673:Metals, Alkaline Earth; D019216:Metals, Heavy; D000469:Alkaline Phosphatase