[An experimental study of the formation of osteoclast-like cell in rat bone marrow culture].
OBJECTIVE
To set up a culture method of osteoclast-like cell (OLC) formation in rat bone marrow and to investigate the effects of 1,25(OH)2D3 and osteoblasts on the formation of OLC.


METHODS
The culture of bone marrow from 4-week old rats were studied for the formation of OLC. The culture was divided into four groups: group A, only bone marrow culture as control; group B, bone marrow and osteoblast (from the crania of newborn rats) coculture; group C, bone marrow culture with 1,25(OH)2D3(final concentration 10(-8) mol/L); group D, bone marrow and osteoblast coculture with 1,25(OH)2D3. After 7 days of culture, the number of OLC which was recognized as tartrate resistant acid phosphatase (TRACP) (+) multinucleated cell, the cellular TRACP and the resorption pits on bone slice were examined.


RESULTS
No OLC was present at the beginning of the bone marrow culture in any groups. OLC still failed to be detected in group A by the end of culture, and a few of OLCs could be found out in group B and C. The number of OLC in group D was more than that in the other groups (P < 0.01). The cellular TRACP in group A was lower than that in the other groups, and the cellular TRACP activity in group B and C was lower than that in group D (P < 0.01). In group D, several smaller resorption pits on The bone slice were observed.


CONCLUSIONS
The formation of OLC requires 1,25(OH)2D3 and coculture with osteoblasts favors the formation of OLC in bone marrow culture.
Institute of Endocrinology, General Hospital of Tianjin Medical University.
J
J
Fan
W
W
Yin
P
P
Cao
D000818:Animals; D001854:Bone Marrow Cells; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D010006:Osteoblasts / Q000166:cytology; D010010:Osteoclasts / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
D002117:Calcitriol