Generation of stable recombinant retroviruses containing the beta-globin genes linked to complex regulatory elements by using transient transfection.
A transient packaging method for a retroviral vector containing beta-globin sequences, mediated by Ca3(PO4)2 precipitation, generated stable recombinant beta-globin retroviruses with intact viral genomes. We suggest the use of this method as an alternative means to overcome the production of beta-globin encoding retroviruses with aberrant viral genomes using the routine packaging procedure.
Li
B
B
Department of Biochemistry & Molecular Biology, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.
Liu
D
D
Wang
J
J
Dong
W
W
Liang
C C
CC
D005822:Genetic Vectors; D005914:Globins / Q000235:genetics*; D016865:Reassortant Viruses; D011995:Recombination, Genetic; D014162:Transfection
D005914:Globins