Transcription factor expression and hormone production in pancreatic AR42J cells.
AR42J is an exocrine pancreatic cell line that has been reported to differentiate towards an endocrine phenotype when stimulated with various growth factors, such as activin A, hepatocyte growth factor (HGF), betacellulin or glucagon-like peptide 1. In our experiments, AR42J-B13 cells differentiated morphologically in response to the growth factor treatment as reported previously. However, they failed to express the insulin gene. We found that the cells did not express several transcription factors known to be found in the beta-cell, including Nkx6.1, isl-1, Pax4 and Pax6. In addition, the mRNA level for pdx-1 and Nkx2.2 were very low in comparison to the insulinoma cell lines INS-1 and RINm5F. However, some transcription factors typically found in beta-cells and neuroendocrine cells were expressed also in the AR42J-B13 cells. These included BETA2/NeuroD, HNF1alpha, C/EBPbeta and IA-1. Unlike the insulinoma cells, AR42J cells expressed the exocrine transcription factor p48. In order to induce endocrine differentiation, we transfected the AR42J-B13 cells with the full length cDNAs of isl-1, Nkx6.1, Nkx2.2 and pdx-1 under the control of the CMV promoter, both separately and in combinations. The expression of Nkx2.2 led consistently to the appearance of pancreatic polypeptide but not insulin, glucagon or somatostatin mRNA. The PP mRNA expression in Nkx2.2 cDNA transfected cells was independent of the growth factor treatment used for differentiating AR42J cells. In conclusion, the AR42J-B13 line possesses some features of a pancreatic neuroendocrine cell. However, we were unable to confirm the capacity of these cells to differentiate into insulin-producing cells. Our results indicate that Nkx2.2 plays a role in the transcriptional regulation of PP expression.
Palgi
J
J
Transplantation Laboratory, Haartman Institute and the Hospital for Children and Adolescents, University of Helsinki, PO Box 21 (Haartmaninkatu 3), FIN-00014, Helsinki, Finland. jaan.palgi@helsinki.fi
Stumpf
E
E
Otonkoski
T
T
D028341:Activins; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D005934:Glucagon / Q000096:biosynthesis / Q000235:genetics; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D000090322:Homeobox Protein Nkx-2.2; D006728:Hormones / Q000096:biosynthesis* / Q000235:genetics; D007265:Inhibins / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis / Q000235:genetics; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection
D017931:DNA Primers; D018076:DNA, Complementary; D000090322:Homeobox Protein Nkx-2.2; D006728:Hormones; D007328:Insulin; C000715628:Nkx2-2 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors; D028341:Activins; D013004:Somatostatin; D007265:Inhibins; D010191:Pancreatic Polypeptide; D017228:Hepatocyte Growth Factor; D005934:Glucagon