The deprotection of Lys(Mtt) revisited.
The selective deprotection of Lys(Mtt)-containing peptidyl resins was successfully monitored by RP-HPLC using very short linear gradients. RP-HPLC analyses of the acidic filtrates also revealed the partial cleavage of the Trt groups and of the peptide-resin bond. The absorbance of the Mtt carbocation at 470 nm is only twice that of the Trt cation. Thus, the UV monitoring at 470 nm seems to be inappropriate, especially at the end of the deprotection, when the Mtt and the Trt levels are comparable.
Bourel
L
L
UMR 8525 CNRS-Université de Lille 2, Institut de Biologie et Institut Pasteur de Lille, France. line.bourel@pasteur-lille.fr
Carion
O
O
Gras-Masse
H
H
Melnyk
O
O
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism; D014320:Triphenylmethyl Compounds / Q000737:chemistry / Q000378:metabolism*; D014466:Ultraviolet Rays
C096179:N(alpha)-9-fluorenylmethoxycarbonyl-N(epsilon)-4-methyltrityllysine; D010455:Peptides; D014320:Triphenylmethyl Compounds; D008239:Lysine