The efficiency of different IRESs (internal ribosomes entry site) in monocistronic mRNAS.
The IRES from poliovirus and from encephalomyocarditis virus (EMCV) added between the cap and the AUG initiator codon were strong inhibitors of chloramphenicol acetyltransferase gene expression in three different cell types. The poliovirus IRES also inhibited bGH (bovine growth hormone) cDNA expression in the HC11 mammary cell line when added between the rabbit whey acidic gene promoter and the cDNA whereas the HTLV-1 IRES showed a stimulatory effect in the same situation. RNA stem loops were added before HTLV-1 (SUR) and the BiP (Immunoglobulin heavy-chain Binding Protein) IRESs followed by the firefly luciferase gene under the control of Rous sarcoma virus (RSV) promoter. The RNA loops abolished the expression of the reporter gene almost completely. These data suggest that the different IRESs may favour or inhibit translation of monocistronic mRNA.
Attal
J
J
Unité de Differenciation Cellulaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Théron
M C
MC
Rival
S
S
Puissant
C
C
Houdebine
L M
LM
D020121:5' Untranslated Regions; D000818:Animals; D016466:CHO Cells; D002352:Carrier Proteins / Q000235:genetics; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D018387:Codon, Initiator; D006224:Cricetinae; D004686:Encephalomyocarditis virus / Q000235:genetics; D000091342:Endoplasmic Reticulum Chaperone BiP; D017930:Genes, Reporter; D006367:HeLa Cells; D006360:Heat-Shock Proteins; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D008894:Milk Proteins / Q000235:genetics; D018832:Molecular Chaperones / Q000235:genetics; D017955:Poliovirus / Q000235:genetics; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012315:RNA Caps; D012333:RNA, Messenger; D011817:Rabbits
D020121:5' Untranslated Regions; D002352:Carrier Proteins; D018387:Codon, Initiator; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D008894:Milk Proteins; D018832:Molecular Chaperones; D012315:RNA Caps; D012333:RNA, Messenger; C024471:whey acidic proteins; D015500:Chloramphenicol O-Acetyltransferase