Determination of cefaclor by selective sample enrichment/clean-up on silica gel bonded polyelectrolyte in ion-exchange column chromatography.
A silica gel-bound cationic polyelectrolyte, poly[N-chloranil N,N,N',N'- tetramethylethylene diammonium dichloride], modified as ion-exchanger capable of molecular recognition of beta-lactam antibiotic, was used in solid phase extraction through column chromatography for a sample clean-up and enrichment of analyte from a dilute solution. The optimum and selective sorption conditions for a model antibiotic, cefaclor, were established. The high selectivity of polymer at pH 9.5 and flow rate as high as 5 ml/min were observed for the quantitative sorption of cefaclor. The desorption by 0.1 N HCl at flow rate of 0.1 ml/min and subsequent heating at 80 degrees C for 2 h allowed the antibiotic to be detected as corresponding oxazolone form in UV-spectrophotometric and differential pulse adsorptive stripping voltammetric measurements. The potential of the suggested approach was illustrated by estimating cefaclor in urine and blood plasma samples.
Gupta
S
S
Dqeartment of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, India.
Prasad
B B
BB
D002433:Cefaclor / Q000032:analysis* / Q000097:blood / Q000652:urine; D002511:Cephalosporins / Q000032:analysis* / Q000097:blood / Q000652:urine; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004573:Electrolytes; D012680:Sensitivity and Specificity; D058428:Silica Gel; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D002511:Cephalosporins; D004573:Electrolytes; D058428:Silica Gel; D002433:Cefaclor; D012822:Silicon Dioxide