Preparative isolation and purification of notopterol and isoimperatorin from Notopterygium forbessi Boiss (Chinese traditional medicinal herb) by high-speed counter-current chromatography.
Preparative high-speed counter-current chromatography was successfully used for isolation and purification of coumarins from Chinese traditional medicinal herb Notopterygium forbessi Boiss (Qianghuo in Chinese) using stepwise elution with a pair of two-phase solvent systems composed of light petroleum-ethyl acetate-methanol-water at volume ratios of 5:5:4.8:5 and 5:5:5:4. Four major components including notopterol and isoimperatorin were isolated, each at over 98% purity.
Beijing Institute of New Technology Application, China.
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F
Yang
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T
Zhang
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Q
Liu
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G
Xu
Y
Y
Zhang
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S
Zhang
Y
Y
Ito
D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011564:Furocoumarins / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C055542:isoimperatorin; C082412:notopterol