Determination of plasma free fatty acids by high-performance liquid chromatography with electrochemical detection.
Determination of free fatty acids (FFAs) in control serum and human plasma was carried out by high-performance liquid chromatography with electrochemical detection. Peak height for palmitic, palmitoleic, stearic, oleic, linoleic, and arachidonic acids at a detection potential of -415 mV vs a saturated calomel electrode showed a linear relation to acid amount in the range 50-1600 pmol. The present method for plasma free fatty acid determination required only 10 microL of plasma sample. The method is simple and the time for blood pretreatment is short. Change in plasma FFA with blood glucose level was monitored before and after meal ingestion by this method, using one male and one female subject. It was possible to rapidly reduce plasma FFA and increase blood glucose subsequent to the meal. The present method is thus shown to have potential for clinical application.
Kotani
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School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Fuse
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T
Kusu
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F
D000328:Adult; D016718:Arachidonic Acid / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D005229:Fatty Acids, Monounsaturated / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D006801:Humans; D019787:Linoleic Acid / Q000097:blood; D008297:Male; D008875:Middle Aged; D009829:Oleic Acids / Q000097:blood; D010169:Palmitic Acids / Q000097:blood; D013229:Stearic Acids / Q000097:blood; D013997:Time Factors
D001786:Blood Glucose; D005229:Fatty Acids, Monounsaturated; D005230:Fatty Acids, Nonesterified; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; C008757:palmitoleic acid; D016718:Arachidonic Acid; D019787:Linoleic Acid