[The structure and mRNA expression of p16 gene in AML cells].
OBJECTIVE
To explore the role of p16 gene in the pathogenesis of AML.


METHODS
PCR-SSCP and Northern blot were used to analyse the structure and mRNA expression of p16 gene in 16 cases of AML.


RESULTS
No deletion or mutation of p16 gene were found in all of the 16 cases. The p16 mRNA expression in 12 AML at diagnosis or relapse was significantly lower than that in 4 long term remission cases and normal controls. p16 mRNA expression was significantly higher in a case of AML with p53 mutation.


CONCLUSIONS
p16 gene may not play an important role in the pathogenesis of AML. The high p16 expression in the patient with p53 mutation might reflect a kind of inbalance of the feedback cycle.
Tongji Hospital, Tongji Medical University, Wuhan.
W
W
Liu
J
J
Zhou
K
K
Xiao
D000293:Adolescent; D000328:Adult; D000368:Aged; D019942:Genes, p16; D016158:Genes, p53; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger