WBC subset analysis of WBC-reduced platelet components.
BACKGROUND
WBC-reduced platelet components may be prepared by filtration or apheresis processing. Both methods have previously been shown to result in a residual total WBC content <5 x 10(6) per component. However, there may be differences in the efficacy of these techniques for removing certain WBC subsets.


METHODS
Two multiparameter flow cytometric assays were developed and validated to perform WBC analysis on WBC-reduced platelets collected with two apheresis instruments (Amicus and COBE Spectra) and on 6 units of filtered pooled random-donor platelet concentrates.


RESULTS
All components contained <1 x 10(5) WBCs. The COBE Spectra and Amicus apheresis platelet components contained more WBCs than did filtered pooled platelets (p<0.05). Lymphocytes (T and B), monocytes, and granulocytes were identified in all components. Granulocyte content was lowest in the Amicus components and filtered pools. Monocytes were lowest in filtered pools. Amicus platelet components had fewer granulocytes and monocytes than the COBE Spectra platelets. Amicus and COBE Spectra components contained more lymphocytes than the filtered pools.


CONCLUSIONS
Multiparameter flow cytometry can be used to quantify and characterize WBCs in WBC-reduced platelet components. WBC reduction by filtration or apheresis was highly effective. WBCs from each subset were identified in all components. Although filtered pools had the lowest numbers of WBCs, the very low numbers observed in all components suggests that the absolute quantitative differences in WBC subset content are of questionable clinical significance.
Triulzi
D J
DJ
Institute for Transfusion Medicine and the Department of Pathology, University of Pittsburgh Medical Center, and the University of Pittsburgh School of Medicine and Graduate School of Public Health, PA 15213, USA. dtriulzi@itxm.org
Meyer
E M
EM
Donnenberg
A D
AD
D001772:Blood Cell Count; D001792:Blood Platelets / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D010983:Plateletpheresis / Q000379:methods*; D012680:Sensitivity and Specificity