Double minutes and c-MYC amplification in acute myelogenous leukemia: Are they prognostic factors?
A case of acute myelogenous leukemia (AML) with double minutes (dmin) and X chromosome loss is presented. Using comparative genomic hybridization (CGH), a region of high-level DNA amplification was detected at 8q24, the locus of the c-MYC proto-oncogene. Fluorescence in situ hybridization (FISH) with a DNA probe specific for the human c-MYC gene confirmed the extrachromosomal amplification of this proto-oncogene in the dmin of the leukemic cells. During the course of the disease, three relapses occurred; two complete remissions could be achieved by treatment with various chemotherapy regimens. The patient's survival time of 25 months was considerably longer than in most reported cases of AML with extrachromosomal c-MYC amplification. Therefore, the present case challenges the view that the occurrence of dmin in AML is generally an indication of poor prognosis.
Department of Human Biology and Human Genetics, University of Kaiserslautern, Kaiserslautern, Germany.
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Bruckert
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Kappler
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Scherthan
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Link
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Hagmann
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Zankl
D000328:Adult; D002872:Chromosome Deletion; D005111:Extrachromosomal Inheritance; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D011379:Prognosis; D000090063:Proto-Oncogene Mas; D014960:X Chromosome