[Exogenous mtsl/p16 gene suppresses tumorigenicity of human gastric cancer cell line].
OBJECTIVE
To determine the effect on cell growth and tumorigenicity of mstl/p16 gene in human gastric cancer cell line.


METHODS
mtsl/p16 gene vector was constructed and transfected into PAMC82 cells which retains down-regulated mtsl/p16 gene expression. Integration and expression of exogenous mtsl/p16 gene were confirmed by PCR, Northern blot, mRNA in situ hybridization and Western blot techniques. Cell morphology was observed in regular culture medium and tumorigenicity in nude mice.


RESULTS
The growth of the cells transferred with mtsl/p16 gene (named as PAMCp16) was dramatically inhibited in nude mice as compared to that of the parental PAMC82 cells. Histologically, the tumor grown from PAMCp16 cells was much better differentiated than that from the parental ones.


CONCLUSIONS
Tumorigenicity of a human gastric cancer cell line in nude mice can be inhibited by transduced exogenous mtsl/p16 gene. mtsl/p16 gene may have potential value in gene diagnosis and gene therapy.
Beijing Institute for Cancer Research.
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M
Sun
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Y
Lu
D000818:Animals; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D019942:Genes, p16; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D014162:Transfection; D014407:Tumor Cells, Cultured
D019941:Cyclin-Dependent Kinase Inhibitor p16; D012333:RNA, Messenger