Investigation of sex chromosome abnormalities in teratozoospermia of infertile men using fluorescence in situ hybridization.
Fluorescence in situ hybridization is a fast and efficient method of investigating chromosomal abnormalities in human spermatozoa. In this study, we have established the frequency of sex chromosome disomy (XX, XY and YY) in teratozoospermia (98-100% abnormal morphology) of infertile men compared with normospermic men using double-probe FISH procedures. A total of 40,000 sperms were scored in each group of semen for chromosome X and Y with overall hybridization efficiency of 97.00 per cent and 98.02 per cent in infertile men and normal men, respectively. It was found that the frequency of disomy XX, XY and YY was significantly higher in infertile men compared with normal men (P < 0.05). It is concluded that the infertile men showed increased frequencies of sex chromosomal abnormalities.
Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
A
A
Aribarg
J
J
Ngeamvijawat
Y
Y
Chanprasit
D000328:Adult; D002869:Chromosome Aberrations / Q000175:diagnosis* / Q000453:epidemiology; D025063:Chromosome Disorders; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015994:Incidence; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000175:diagnosis* / Q000235:genetics*; D011336:Probability; D012016:Reference Values; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012730:Sex Chromosomes; D018709:Statistics, Nonparametric