Expression of LFA-1 (CD11a/CD18) and ICAM-1 (CD54) in an animal model of renal interstitial fibrosis induced by unilateral ureteral obstruction.
Unilateral ureteral obstruction (UUO) has been used as an experimental model to induce tubulointerstitial damage and interstitial fibrosis. UUO is characterized by cellular proliferation, accumulation of inflammatory cells, and subsequent replacement of renal parenchyma by fibrous tissue. The influx of inflammatory cells into the renal interstitium is mediated by adhesion molecules. In this study, the development of fibrosis in the UUO model of the rat was examined and its relation to the time course of LFA-1 and ICAM-1 expression was assessed by immunohistochemistry. An increase in interstitial connective tissue was detected on day 10 after UUO, with a maximum on day 35. After unilateral ureteral obstruction, LFA-1 was prominently expressed in interstitial infiltrates, and to a lesser degree in glomerular areas. An initial increase in LFA-1-positive cells was noted already on day 10, with a maximum on day 20 and a decline on day 25. During the time course of 35 days after UUO, we observed an increase in ICAM-1 expression in the vascular endothelium, in tubular epithelium and in interstitial areas. This study shows that LFA-1 expression and ICAM-1 expression are concordant and that this process is associated with increasing interstitial fibrosis. ICAM-1 interstitial tissue may facilitate the homing and persistence of an interstitial infiltrate by ICAM-1/LFA-1 interactions, thereby preceding the development of renal interstitial fibrosis.
Institute of Pathology, Friedrich Schiller University Jena, Germany.
J
J
Kriegsman
H
H
Müller
M
M
Sommer
K
K
Stiller
R
R
Bräuer
P K
PK
Petrow
A
A
Gaumann
R D
RD
Fünfstück
G
G
Stein
D000818:Animals; D018821:CD18 Antigens / Q000032:analysis*; D004195:Disease Models, Animal; D005355:Fibrosis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D007668:Kidney / Q000737:chemistry / Q000473:pathology*; D008026:Ligation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis*; D008297:Male; D009395:Nephritis, Interstitial / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014513:Ureter / Q000601:surgery; D014517:Ureteral Obstruction / Q000150:complications*
D018821:CD18 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1