Space flight is associated with rapid decreases of undercarboxylated osteocalcin and increases of markers of bone resorption without changes in their circadian variation: observations in two cosmonauts.
BACKGROUND
Microgravity induces bone loss by mechanism(s) that remain largely unknown.


METHODS
We measured biochemical markers related to bone remodeling in two cosmonauts before, during, and after 21- and 180-day space flights, respectively.


RESULTS
During both flights, type I procollagen propeptide and bone alkaline phosphatase decreased as early as 8 days after launch. Undercarboxylated osteocalcin percentage increased early and remained high during both flights. Vitamin K supplementation restored carboxylation of osteocalcin during the long-term flight. Urinary and serum C-telopeptide of type I collagen (CTX) increased as early as day 8 of the flights; the increase was greater in serum than in urine. Pyridinoline, free deoxypyridinoline, and N-telopeptide increased less than CTX during the short-term space flight. The circadian rhythm of bone resorption assessed by urine CTX and free deoxypyridinoline was not altered by microgravity.


CONCLUSIONS
Vitamin K metabolism or action and bone remodeling may be altered in cosmonauts.
Bone Biology Laboratory, Faculté de Médecine, 42023 Saint-Etienne cedex 2, France.
A
A
Caillot-Augusseau
L
L
Vico
M
M
Heer
D
D
Voroviev
J C
JC
Souberbielle
A
A
Zitterman
C
C
Alexandre
M H
MH
Lafage-Proust
D000328:Adult; D000469:Alkaline Phosphatase / Q000652:urine; D000596:Amino Acids / Q000097:blood / Q000652:urine*; D018480:Astronauts; D015415:Biomarkers / Q000652:urine; D001862:Bone Resorption / Q000652:urine*; D001842:Bone and Bones / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D002940:Circadian Rhythm; D003094:Collagen / Q000097:blood / Q000652:urine*; D024042:Collagen Type I; D006801:Humans; D008297:Male; D015675:Osteocalcin / Q000652:urine*; D010455:Peptides / Q000097:blood / Q000652:urine*; D011347:Procollagen / Q000652:urine; D013026:Space Flight; D013997:Time Factors; D014812:Vitamin K / Q000008:administration & dosage
D000596:Amino Acids; D015415:Biomarkers; D024042:Collagen Type I; D010455:Peptides; D011347:Procollagen; C052929:collagen type I trimeric cross-linked peptide; D015675:Osteocalcin; D014812:Vitamin K; C036020:deoxypyridinoline; D003094:Collagen; D000469:Alkaline Phosphatase