Hypochlorous acid and human blood low density lipoproteins modified by hypochlorous acid increase erythrocyte adhesion to endothelial cells.
The ability of hypochlorous acid (HOCl) (anion form - hypochlorite, OCl-) and HOCl/OCl- -modified human blood low density lipoproteins (HOCl-LDLs) to stimulate erythrocyte adhesion to endothelial cell monolayers was studied. LDLs were modified by incubating at different HOCl/OC- concentrations. This led to a damage of proteins and lipids. We found (1) a more than 20-fold decrease of LDL fluorescence intensity (extinction at 285 nm, emission at 340 nm), (2) accumulation of secondary (TBA-reactive substances) and final (Schiff bases) products of lipid peroxidation, and (3) increase in the electrophoretic mobility of LDLs. Preincubation of endothelial cells (ECs) with HOCI/OCl- (up to 50 microM) enhanced erythrocyte adhesion to the EC monolayer. Preincubation of ECs with HOCl-LDLs (up to 250 microM of HOCI//OCl- during LDL modification) (1) caused an increase in the cholesterol/phospholipid molar ratio in EC and (2) enhanced adhesion of erythrocytes to endothelium. Application of HOCl/OCl- at concentrations above 50 microM or treatment of LDLs with 500 microM HOCl resulted in the cytotoxic effect on ECs and led to a decrease in the molar cholesterol/phospholipid ratio in ECs and adhesion of erythrocytes to endothelium. The results suggest that HOCl/OCl- at physiological concentrations stimulates the adhesion of blood cells to the endothelium and cholesterol accumulation in the vessel wall ECs either directly or due to LDL modification. Both effects could be important in the development of many vascular diseases.
Research Institute of Physico-Chemical Medicine, Moscow, Russia.
E A
EA
Gorbatenkova
G M
GM
Artmann
O M
OM
Panasenko
D001161:Arteriosclerosis / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008077:Lipoproteins, LDL / Q000097:blood* / Q000187:drug effects / Q000494:pharmacology
D008077:Lipoproteins, LDL; D006997:Hypochlorous Acid; D002784:Cholesterol