Quantitative phase determination for macromolecular crystals using stereoscopic multibeam imaging.
Without invoking anomalous dispersion and heavy-atom derivatives, it is demonstrated that it is possible to directly determine the phases of a large number of reflections collected in a short time from macromolecular crystals using a stereoscopic oscillation-crystal imaging technique, in a multibeam diffraction geometry, where two crystallographic axes in opposite directions are employed as the rotation axes. The intensity profiles (distributions) of the diffraction spots versus the varying tilt Bragg angle of the rotation axis in the two stereoscopically related images yield quantitative phase information. Many multiple diffraction profiles of tetragonal lysozyme and an unknown protein structure are obtained at the rate of 100 profiles per 30 min of X-ray exposure.
Department of Physics, National Tsing Hua University, Hsinchu, Taiwan 300. slchang@phys.nthu.edu.tw
SL
Chang
CH
Chao
YS
Huang
YC
Jean
HS
Sheu
FJ
Liang
HC
Chien
CK
Chen
HS
Yuan