Membrane permeability modifications are involved in antibiotic resistance in Klebsiella pneumoniae.
Two Klebsiella pneumoniae strains selected according to their high cross-resistance pattern to cephalosporins were characterized by (i) outer membrane protein content such as OmpA or nonspecific porins, (ii) MICs of various cephalosporins and unrelated antibiotics, (iii) beta-lactamase production, and (iv) active efflux of fluoroquinolones. An association of porin deficiency and beta-lactamase production induced a noticeable cephalosporin resistance. In addition to these mechanisms, the presence of an active efflux participating in high-level fluoroquinolone resistance was identified in one strain. The decrease of antibiotic uptake associated with efflux explains the Klebsiella adaptation against the drugs present in the environment.
Faculté de Médecine, CJF 9606 INSERM, Université de la Méditerranée, 27 Boulevard Jean Moulin, Marseille Cedex 05, 13385, France.
J
J
Chevalier
J M
JM
Pagès
A
A
Eyraud
M
M
Malléa
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D004352:Drug Resistance, Microbial / Q000502:physiology; D015378:Imipenem / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009643:Norfloxacin / Q000493:pharmacokinetics; D001618:beta-Lactamases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001425:Bacterial Outer Membrane Proteins; D015378:Imipenem; D001618:beta-Lactamases; D009643:Norfloxacin