High levels of apoptosis are associated with improved survival in non-small cell lung cancer.
Tumour growth is accompanied by angiogenesis and reduced apoptosis in experimental animals. The aim of this study was to examine the prognostic value of apoptosis and the association between apoptosis and vascularity in non-small cell lung cancer (NSCLC). Following in-situ end-labelling of DNA, apoptotic cells were quantified by three different indices: as a percentage, either counting total cells (AI-tc) or point-counting (AI-pc), or as cells per area (AI-area). Blood vessels were stained with vWF antibody and vascularity was quantified by three methods. Median values for AI-tc, AI-pc and AI-area were 0.38, 0.32 and 10.7, respectively. High values were associated with improved survival, reaching statistical significance for AI-area (p < 0.05). All three apoptotic indices were significantly correlated with each other, but no correlation was found between indices of apoptosis and vascularity. As previously reported, vascularity had no prognostic value. These results indicate that, in NSCLC, vascularity is not informative, but apoptotic index may be a useful prognostic factor.
Oral Diseases Group, Dental School, University of Dundee, U.K.
M
M
Macluskey
R
R
Baillie
L M
LM
Chandrachud
N
N
Pendleton
A M
AM
Schor
D000230:Adenocarcinoma / Q000098:blood supply / Q000401:mortality / Q000473:pathology; D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000098:blood supply / Q000401:mortality* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000098:blood supply / Q000401:mortality / Q000473:pathology; D002452:Cell Count; D006801:Humans; D020287:In Situ Nick-End Labeling; D015993:Life Tables; D008175:Lung Neoplasms / Q000098:blood supply / Q000401:mortality* / Q000473:pathology; D009389:Neovascularization, Pathologic; D011379:Prognosis; D016019:Survival Analysis