Determination of the minor component bromhexine in cotrimoxazole-containing tablets by absorption spectrophotometry and partial least-squares (PLS-1) multivariate calibration.
The mucolitic bromhexine [N-(2-amino-3,5-dibromobenzyl)-N-methylcyclohexylamine] has been determined in cotrimoxazole-containing tablets by partial least-squares (PLS-1) multivariate of spectrophotometric calibration data in the spectral range 310-350 nm. In the studied commercial tablets, cotrimoxazole is present in large excess (ca. 100:1 in weight) with respect to bromhexine, and a high degree of spectral overlapping exists among bromhexine and cotrimoxazole components. However, the obtained recoveries are reasonably good with the presently discussed technique.
Departamento de Química Analítica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
M E
ME
Ribone
A P
AP
Pagani
A C
AC
Olivieri
D001964:Bromhexine / Q000032:analysis*; D002138:Calibration; D016018:Least-Squares Analysis; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013607:Tablets / Q000032:analysis*; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000032:analysis*
D013607:Tablets; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D001964:Bromhexine