Identification of group X secretory phospholipase A(2) as a natural ligand for mouse phospholipase A(2) receptor.
Phospholipase A(2) receptor (PLA(2)R) mediates various biological responses elicited by group IB secretory phospholipase A(2) (sPLA(2)-IB). The recently cloned group X sPLA(2) (sPLA(2)-X) possesses several structural features characteristic of sPLA(2)-IB. Here, we detected a specific binding site of sPLA(2)-X in mouse osteoblastic MC3T3-E(1) cells. Cross-linking experiments demonstrated its molecular weight (180 kDa) to be similar to that of PLA(2)R. In fact, sPLA(2)-X was found to bind the recombinant PLA(2)R expressed in COS-7 cells, and its specific binding detected in mouse lung membranes was abolished by the deficiency of PLA(2)R. These findings demonstrate sPLA(2)-X to be one of the high-affinity ligands for mouse PLA(2)R.
Shionogi Research Laboratories, Shionogi and Co. Ltd., 12-4 Sagisu, 5-Chome, Fukushima-ku, 553-0002, Osaka, Japan.
Y
Y
Yokota
K
K
Higashino
K
K
Nakano
H
H
Arita
K
K
Hanasaki
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D003432:Cross-Linking Reagents; D054501:Group II Phospholipases A2; D008024:Ligands; D008168:Lung / Q000166:cytology; D051379:Mice; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D054507:Receptors, Phospholipase A2; D011994:Recombinant Proteins / Q000378:metabolism; D013552:Swine; D013816:Thermodynamics; D014162:Transfection
D003432:Cross-Linking Reagents; D008024:Ligands; C516357:Pla2r1 protein, mouse; D011956:Receptors, Cell Surface; D054507:Receptors, Phospholipase A2; D011994:Recombinant Proteins; D010741:Phospholipases A; D054501:Group II Phospholipases A2