Determination of bile acids in pharmaceutical formulations using micellar electrokinetic chromatography.
A micellar electrokinetic chromatography method (MEKC) has been developed and validated for the determination of bile acids (BA) such as ursodeoxycholic acid (UDCA), dehydrocholic acid (DHCA) and deoxycholic acid (DCA) in pharmaceuticals for quality control purpose. The background electrolyte consisted of 20 mM borate-phosphate buffer containing 50 mM sodium dodecylsulfate (SDS), and acetonitrile as additive. UV detection was set at 185 nm. Selectivity, linearity, range, repeatability, intermediate precision and accuracy showed good results. Comparison of the values obtained by MEKC and HPLC methods were in close agreement.
Department of Analytical and Physical Chemistry, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
V G
VG
Rodríguez
S E
SE
Lucangioli
G C
GC
Fernández Otero
C N
CN
Carducci
D001647:Bile Acids and Salts / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D001647:Bile Acids and Salts; D004364:Pharmaceutical Preparations