Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle.
The monoclonal antibody to N(epsilon)-(hexanonyl)lysine (HEL), a novel adduct formed by the reaction of linoleic acid hydroperoxide and lysine, has been prepared and characterized. The obtained antibody specifically recognized the HEL moiety. Using the monoclonal antibody, we evaluated the protective effects of feeding eriocitrin, which is one of flavonoids in lemon fruit, on oxidative modification induced by exercise in rats. The supplementation of eriocitrin significantly suppressed the increase in HEL in the skeletal muscle by exercise. The result suggests that the determination of HEL may be a good method for evaluation of the protective effect of beneficial food factors against oxidative stress.
School of Humanities for Environmental Policy and Technology, Himeji Institute of Technology, Himeji, 670-0092, Japan.
Y
Y
Kato
Y
Y
Miyake
K
K
Yamamoto
Y
Y
Shimomura
H
H
Ochi
Y
Y
Mori
T
T
Osawa
D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002208:Caproates / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D019587:Dietary Supplements; D005260:Female; D044950:Flavanones; D005419:Flavonoids / Q000008:administration & dosage; D006569:Hesperidin / Q000008:administration & dosage* / Q000031:analogs & derivatives*; D008054:Lipid Peroxides / Q000737:chemistry; D008239:Lysine / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D005082:Physical Exertion; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000447:Aldehydes; D000911:Antibodies, Monoclonal; D002208:Caproates; D002234:Carbodiimides; D044950:Flavanones; D005419:Flavonoids; D008054:Lipid Peroxides; C120589:N(epsilon)-hexanoyllysine; C037652:hexanoic acid; D012710:Serum Albumin, Bovine; C114706:eriocitrin; D006569:Hesperidin; D008239:Lysine