The biphasic expression of IMP/Vg1-RBP is conserved between vertebrates and Drosophila.
The human IGF-II mRNA-binding proteins (IMPs) 1-3, and their Xenopus homologue Vg1 RNA-binding protein (Vg1-RBP) are RNA-binding proteins implicated in mRNA localization and translational control in vertebrate development. We have sequenced the Drosophila homologue (dIMP) of these genes, and examined its expression pattern in Drosophila embryos by in situ hybridization. The study shows that dIMP exhibits a biphasic expression pattern. In the early stages of development, a maternal pool of dIMP mRNA is evenly distributed in the embryo and degraded by the end of stage 4. Expression reappears in the developing central nervous system, where dIMP is expressed throughout neurogenesis. In addition, dIMP is present in the pole cells.
Institute of Molecular Biology, University of Copenhagen, Solvgade 83 H, DK-1307 K, Copenhagen, Denmark.
J
J
Nielsen
F
F
Cilius Nielsen
R
R
Kragh Jakobsen
J
J
Christiansen
D000595:Amino Acid Sequence; D000818:Animals; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins; D020224:Expressed Sequence Tags; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D029721:Drosophila Proteins; C498778:Imp protein, Drosophila; D016601:RNA-Binding Proteins