Angiogenesis in a patient with ischemic limb induced by intramuscular injection of vascular endothelial growth factor and fibrin platform.
Direct revascularization of critical ischemia of the limb is often unsuccessful due to the anatomic extent and distribution of arterial occlusive disease, and no pharmacologic treatment has proved effective in treating this condition. Patients with ischemic limb may eventually require amputation and may develop serious morbidity and mortality. The goal of limb salvage in these patients has stimulated research into alternative treatment methods, including angiogenesis. Attempts have been made to apply growth factors directly or to encode DNA for such factors, but it is unknown whether these factors remain at the target site long enough to be effective. We report our strategy of using vascular endothelial growth factor in a fibrin network, which enables the sustained release of biologic material at the target site.
Medical College of Wisconsin, Milwaukee, USA.
N
N
Kipshidze
V
V
Chekanov
P
P
Chawla
L R
LR
Shankar
J B
JB
Gosset
K
K
Kumar
D
D
Hammen
J
J
Gordon
M H
MH
Keelan
D000368:Aged; D016228:Endothelial Growth Factors / Q000008:administration & dosage / Q000627:therapeutic use*; D015718:Fibrin Tissue Adhesive; D006801:Humans; D007273:Injections, Intramuscular; D007511:Ischemia / Q000628:therapy*; D007866:Leg / Q000098:blood supply*; D008222:Lymphokines / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D020033:Protein Isoforms; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D015718:Fibrin Tissue Adhesive; D008222:Lymphokines; D020033:Protein Isoforms; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors