IL-6 protein production by airway epithelial(-like) cells disabled in IL-6 mRNA degradation.
IL-6 mRNA and protein expression in human airway epithelial-like H292 cells depends on rapid, but regulable IL-6 mRNA degradation. We restricted IL-6 mRNA degradation by partially inhibiting protein synthesis and studied the IL-6 response. Despite partial inhibition of protein synthesis, IL-6 protein production was increased and prolonged. Furthermore, the threshold concentration for stimuli of IL-6 protein production decreased and the dose-response curves became steeper. Similar findings were obtained with primary human bronchial epithelial cells. This exaggerated production may apply to other proteins encoded by labile mRNA and is likely to occur during viral infection of airway epithelial cells.
Department of Pulmonology, F4-206, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands. r.lutter@amc.uva.nl
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Lutter
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Loman
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Snoek
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Roger
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Out
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Jansen
D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism
D015850:Interleukin-6; D012333:RNA, Messenger; D003513:Cycloheximide