In vivo detection of vascular adhesion protein-1 in experimental inflammation.
Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.
National Public Health Institute and MediCity Research Laboratory, the Department of Medicine, Nuclear Medicine, and Pharmacology and Clinical Pharmacology, the University of Turku, Turku, Finland.
K
K
Jaakkola
T
T
Nikula
R
R
Holopainen
T
T
Vähäsilta
M T
MT
Matikainen
M L
ML
Laukkanen
R
R
Huupponen
L
L
Halkola
L
L
Nieminen
J
J
Hiltunen
S
S
Parviainen
M R
MR
Clark
J
J
Knuuti
T
T
Savunen
P
P
Kääpä
L M
LM
Voipio-Pulkki
S
S
Jalkanen
D006631:Amine Oxidase (Copper-Containing) / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D004285:Dogs; D015902:Gamma Cameras; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D007457:Iodine Radioisotopes; D051379:Mice; D011877:Radionuclide Imaging; D012867:Skin / Q000737:chemistry / Q000000981:diagnostic imaging / Q000473:pathology; D013552:Swine; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007457:Iodine Radioisotopes; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing)