On the influence of pigment-protein interactions on energy transfer processes in photosynthetic membrane structures. 3. The FMO complex of Chlorobium tepidum at high pressure.
The low-temperature absorption spectra of the Chlorobium tepidum FMO bacteriochlorophyll-protein complex at various pressures have been calculated within the framework of mini-exciton theory. The dependences of the Qy transition energies of the monomeric pigments on pressure have been found by means of functional minimization. This functional includes the parameters of both theoretical and experimental absorption spectra at low temperatures and various pressures. The dependences obtained are compared with those derived for the exciton transition energies, which have been obtained by deconvoluting absorption spectra with seven Gaussian components at each pressure. The pressure increase has been shown to result in the increased coupling energy between both the pigment molecules themselves and pigments and amino acid residues. The pigment molecules capable of binding histidines and water molecules have been shown to have the greatest and smallest responses to increased pressure, respectively. The couplings of Bchl molecules with the surrounding amino acid residues have been shown to change both the exciton delocalization index and the exciton distribution between the pigment molecules within the protein subunit; the increased pressure does not change these parameters significantly.
Institute of Soil Science and Photosynthesis, Russian Academy of Sciences, Pushchino, Moscow Region.
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Klevanik
D001426:Bacterial Proteins; D021961:Cell Membrane Structures / Q000378:metabolism*; D019414:Chlorobi / Q000378:metabolism*; D004735:Energy Transfer / Q000502:physiology*; D055537:Light Signal Transduction / Q000502:physiology; D045342:Light-Harvesting Protein Complexes; D008954:Models, Biological; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D011312:Pressure; D017434:Protein Structure, Tertiary / Q000502:physiology; D013054:Spectrophotometry, Atomic
D001426:Bacterial Proteins; C086320:FMO bacteriochlorophyll protein, Bacteria; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins