[Measurement of thyrotropin receptor antibodies (TRAK) with a second generation assay in patients with Graves' disease].
OBJECTIVE
The detection of TSH-receptor-antibodies (TRAb) in patients (pts) with Graves' disease (GD) is routinely used in nuclear medicine laboratories. It is performed by commercial, porcine radioreceptorassays (RRA) measuring TSH binding inhibitory activity. A second generation assay using the human, recombinant TSH-receptor was developed during the last years. The manufacturer composed this new assay as a coated tube RRA (CT RRA) and claimed a higher sensitivity for GD.


METHODS
TRAb was measured in 207 pts with various thyroid disorders and 205 healthy controls using the new coated tube RRA (Fa. B.R.A.H.M.S. Diagnostica GmbH, Berlin, Germany) as well as a conventional RRA (Fa. Medipan Diagnostica GmbH, Selchow, Germany): 60 pts suffering from GD showing a relapse after antithyroid drug treatment and before radioiodine therapy, 109 pts with disseminated autonomia (DA) and 38 pts suffering from Hashimoto's thyroiditis. A ROC-analysis was performed to find the optimal decision threshold level for positivity.


RESULTS
We found 42/60 TRAb-positive pts with GD in the established RRA (threshold 6 U/L) and 52/60 in the CT RRA, respectively. The sensitivity increased from 70% (RRA) to 86.7% (CT RRA). The CT RRA found 2 false positives (one Hashimoto's and one healthy control) and the RRA detected 3 Hashimoto's and 2 healthy controls as false positive.


CONCLUSIONS
The increased sensitivity of CT RRA for GD provides an advantage compared to conventional RRA, especially in GD-patients relapsing after antithyroid drug treatment. Functional sensitivity and Interassay-variation of CT RRA are very precisely compared to conventional RRA. Handling of the new assay is also improved.
Klinik und Poliklinik für Nuklearmedizin, Universitätsklinikum Carl Gustav Carus, Technischen Universität Dresden, Deutschland. kzophel@rcs.urz.tu-dresden.de
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K
Zöphel
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Wunderlich
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Koch
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WG
Franke
D005189:False Positive Reactions; D006111:Graves Disease / Q000097:blood / Q000276:immunology*; D006801:Humans; D018828:Immunoglobulins, Thyroid-Stimulating / Q000097:blood*; D012372:ROC Curve; D011869:Radioligand Assay; D011989:Receptors, Thyrotropin / Q000276:immunology; D012008:Recurrence; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013959:Thyroid Diseases / Q000097:blood / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000276:immunology
D018828:Immunoglobulins, Thyroid-Stimulating; D011989:Receptors, Thyrotropin