Occurrence and taxonomic significance of oxo-fatty acids in lipopolysaccharides from members of Mesorhizobium.
Lipopolysaccharides (LPSs) isolated from seven strains of Mesorhizobium were studied for the presence of fatty acids with particular attention for 27-oxooctacosanoic acid and 4-oxo fatty acids. The LPSs from all analysed strains contained various amounts of 27-oxo-28:0 and all of them, with the exception of Mesorhizobium tianshanense, contained also 4-oxo fatty acids (4-oxo-20:0, 4-oxo-i-21:0, 4-oxo-22:0). The group of amide-linked fatty acids consisted of a wide range of 3-hydroxylated and 4-oxo fatty acids whereas all the nonpolar as well as the (omega-1) hydroxylated long-chain acids and the 27-oxo-28:0 fatty acids were ester-linked. The characteristic spectrum of 3-hydroxy fatty acids and presence of 27-OH-28:0 as well as 27-oxo-28:0 acid in LPSs of Mesorhizobium showed that these strains were closely related. Therefore the lipid A fatty acid pattern could be a useful chemotaxonomic marker which helps to isolate the Mesorhizobium group from rhizobium bacteria during the classification process.
Department of General Microbiology, Maria Curie-Skłodowska University, Lublin, Poland. achoma@biotop.umcs.lublin.pl
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A
Choma
T
T
Urbanik-Sypniewska
R
R
Russa
J
J
Kutkowska
H
H
Mayer
D000577:Amides / Q000737:chemistry; D015373:Bacterial Typing Techniques; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000302:isolation & purification*; D008050:Lipid A / Q000737:chemistry*; D010944:Plants / Q000382:microbiology; D012230:Rhizobiaceae / Q000737:chemistry / Q000145:classification*
D000577:Amides; D004952:Esters; D005227:Fatty Acids; D008050:Lipid A