Early physiological and cytological events induced by wounding in potato tuber.
The response of potato tuber (Solanum tuberosum L. cv. Kennebec) to mechanical wounding was investigated at different times. Changes in the levels of indole-3-acetic acid (IAA), polyunsaturated fatty acids (PUFAs) and lipid hydroperoxides (LOOHs) were monitored up to 120 min after wounding and related to the cytological events occurring up to 24 h. Twenty minutes after injury, an increase in IAA and LOOH levels and a decrease in the levels of PUFAs was observed. Wounding induced mitoses in differentiated (parenchyma) cells starting at 120 min, and promoted an increase of mitotic activity in the meristematic cells (procambium and bud dome), after 360 min. The inhibition of the increase in LOOHs and IAA by lipoxygenase (LOX) inhibitors, as well as the ability of in vitro peroxidated linoleic acid to enhance IAA production, suggest a close relationship among lipoperoxidation, IAA and mitotic activity in the response of potato tuber cells to injury, resulting in a specific growth response, i.e. bud growth and periderm formation.
Fabbri
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Dipartimento Biologia Vegetale, Università La Sapienza, Rome, Italy. afabbri@axrma.uniroma1.it
Fanelli
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C
Reverberi
M
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Ricelli
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Camera
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E
Urbanelli
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S
Rossini
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A
Picardo
M
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Altamura
M M
MM
D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism*; D007527:Isoenzymes / Q000032:analysis; D019787:Linoleic Acid / Q000737:chemistry; D008054:Lipid Peroxides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism*; D012457:Salicylamides / Q000494:pharmacology; D011198:Solanum tuberosum / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D017962:alpha-Linolenic Acid / Q000737:chemistry
D005231:Fatty Acids, Unsaturated; D007210:Indoleacetic Acids; D007527:Isoenzymes; D008054:Lipid Peroxides; D016859:Lipoxygenase Inhibitors; D010937:Plant Growth Regulators; D012457:Salicylamides; D017962:alpha-Linolenic Acid; C030737:indoleacetic acid; C005703:salicylhydroxamic acid; D019787:Linoleic Acid; D008084:Lipoxygenase