Corf, the Rev/Rex homologue of HTDV/HERV-K, encodes an arginine-rich nuclear localization signal that exerts a trans-dominant phenotype when mutated.
Regulation of nucleo-cytoplasmic export of viral transcripts by a viral protein (Rev/Rex) is a characteristic feature in the replication cycle of complex retroviruses. We recently reported that the endogenous retrovirus family HTDV/HERV-K encodes a protein, Corf, that is a cellular Counterpart of Rev/Rex function and thus a new component of nucleo-cytoplasmic pathways. In HTDV/HERV-K-expressing cells, Corf is localized within the nucleoli. Here we describe the nuclear localization signal (NLS) of the Corf protein. Mutations in the NLS lead to cytoplasmic accumulation of the mutated protein and abrogate Corf function in a trans-dominant way.
Magin
C
C
Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.
Hesse
J
J
Löwer
J
J
Löwer
R
R
D000595:Amino Acid Sequence; D001120:Arginine / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D001692:Biological Transport; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D018076:DNA, Complementary; D020077:Endogenous Retroviruses; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D015694:Gene Products, rev; D016370:Gene Products, rex; D005799:Genes, Dominant; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D019913:Nuclear Localization Signals / Q000235:genetics / Q000502:physiology*; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D018076:DNA, Complementary; D005231:Fatty Acids, Unsaturated; D015694:Gene Products, rev; D016370:Gene Products, rex; C406149:HERV-K cORF protein, Human endogenous retrovirus K; D019913:Nuclear Localization Signals; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D001120:Arginine; C038753:leptomycin B