Reaction rate coefficients of hydroxyl radical-induced DNA single- and alpha-type double-strand breaks.
With a model system of pBR322 plasmid DNA solution in vitro, the dose effects of radiation-induced single- and double-strand breaks (SSB and DSB) were measured and DSB was distinguished into alpha- and beta-types. Under the condition of low scavenging capacity existing in the irradiated DNA solution, SSB and alpha DSB were mainly induced by hydroxyl radicals (.OH). Moreover, a certain relationship was obtained between the SSB and alpha DSB yields and the DNA concentration. It was found that when the DNA solution was irradiated in the presence of 2.5 mmol dm-3 mannitol, the reciprocals of G(SSB) and G(alpha DSB), respectively, were linearly related to the reciprocal of the DNA concentration, i.e. the competition reactions of DNA and mannitol for .OH radicals can be described by second-order kinetics. The rate coefficients and the efficiencies of the .OH radical inducing SSB were deduced. Also, the reaction rate coefficients and the efficiencies for the induction of alpha DSB from SSB by the .OH radical transfer mechanism, were first derived from the competition kinetics.
Shao
C
C
Laboratory of Ion Beam Bioengineering, Academia Sinica, Hefei, PR China. CLShao@mail.ipp.ac.cn
Yu
Z
Z
Saito
M
M
D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004586:Electrophoresis; D017665:Hydroxyl Radical; D007700:Kinetics; D015233:Models, Statistical; D010957:Plasmids / Q000528:radiation effects*
D017665:Hydroxyl Radical; D004247:DNA