A role for TNF in bone resorption of deciduous molars in human beings.
In this study, tumor necrosis-alpha was sampled from the gingival crevice of human deciduous molars; this was compared with values measured from the crevice of those deciduous molars missing a permanent successor, and from the crevice of deciduous ankylosed molars. Tumor necrosis-alpha was harvested from the gingival crevice with magnetic microspheres coated with tumor necrosis-alpha antibodies. The amount of bead-bound tumor necrosis-alpha was quantified with the use of an enzyme-linked immunosorbent assay. One hundred seven sites (from 41 patients) were sampled; for each patient, the normal value was compared with either the molars missing a permanent successor or ankylosed value. The tumor necrosis-alpha levels were 1.6 times higher from the crevice of ankylosed deciduous molars when compared with normal deciduous molars and 2.6 times higher from the crevice of sites with a molar missing a permanent successor. The mean and standard error mean distribution of tumor necrosis-alpha expressed as picograms was: normal molars 91 pg (standard error mean +/- 20), ankylosed molars 150 pg (standard error mean +/- 31), and missing permanent successor 236 pg (standard error mean +/- 67). Analysis of variance showed the difference among the 3 means was close to attaining significant difference (F [2.104] = 2.7905, P =.066). Multiple comparison procedures indicated that the mean for molars missing a permanent successor and the normal groups were significantly different, P =.05. The results of this study suggest tumor necrosis-alpha values are elevated in the gingival crevice of deciduous molars with ankylosis and where the permanent tooth bud is congenitally missing.
School of Dental Medicine, University of Connecticut Health Center, USA. Sureles@aol.com
S D
SD
Ureles
J M
JM
Chrzan
L A
LA
Norton
E F
EF
Rossomando
D000539:Alveolar Process / Q000378:metabolism*; D000848:Anodontia / Q000378:metabolism; D002648:Child; D003795:Dental Sac / Q000002:abnormalities / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005881:Gingiva / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D008963:Molar / Q000378:metabolism; D020254:Tooth Ankylosis / Q000378:metabolism*; D014078:Tooth Eruption / Q000502:physiology*; D020390:Tooth Socket / Q000737:chemistry; D014094:Tooth, Deciduous / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism / Q000502:physiology*
D014409:Tumor Necrosis Factor-alpha