Immuno-crossreactivity and toxicity assessment of conjugation products of the cyanobacterial toxin, microcystin-LR.
Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxin and glutathione, cysteine-glycine and cysteine. The antibodies against microcystin-LR crossreacted with the toxin conjugation products with similar affinities (96-112%) to that of microcystin-LR, when assayed at a concentration of 1 microg l(-1). Toxicity assessment of the conjugates, in comparison to microcystin-LR, indicated a reduction according to mouse bioassay. In vitro protein phosphatase inhibition assay indicated that the conjugates possessed approximately 3-9-fold lower toxicity than microcystin-LR.
Department of Biological Sciences, University of Dundee, Dundee DD1 4HN, UK.
J S
JS
Metcalf
K A
KA
Beattie
S
S
Pflugmacher
G A
GA
Codd
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D001427:Bacterial Toxins / Q000276:immunology* / Q000494:pharmacology; D003429:Cross Reactions; D003545:Cysteine / Q000276:immunology; D005978:Glutathione / Q000276:immunology; D005998:Glycine / Q000276:immunology; D008099:Liver / Q000187:drug effects; D008387:Marine Toxins; D051379:Mice; D008807:Mice, Inbred BALB C; D052998:Microcystins; D010456:Peptides, Cyclic / Q000276:immunology* / Q000494:pharmacology
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C057862:cyanoginosin LR; D005978:Glutathione; D003545:Cysteine; D005998:Glycine