Short-term tracheal occlusion corrects pulmonary vascular anomalies in the fetal lamb with diaphragmatic hernia.
BACKGROUND
Sustained fetal tracheal occlusion (TO) results in accelerated lung growth but causes severe type II cell depletion. Temporary TO fails to cause lung growth in a congenital diaphragmatic hernia (CDH) model but preserves type II cells and corrects pulmonary hypertension. Herein, we study the pulmonary vascular changes caused by temporary TO.


METHODS
CDH was created in 12 fetal lambs (65-70 d; term, 145 days). In 6 lambs, the trachea was occluded for 2 weeks (CDH + TO; 108-122 d). Animals were killed at 136 days. The lungs were processed with elastin stains and anti-alpha-smooth muscle actin antibody. Partial or circumferential presence of inner and outer elastic lamina was used to determine muscularization of pulmonary arterioles. The percent of medial wall thickness was plotted against vessel diameter for each group.


RESULTS
Lung weight/body weight was smaller in lambs with CDH (1. 35% +/- 0.56%) and CDH + TO (1.70% +/- 0.34%) than in control lambs (3.55% +/- 0.56%; P <.05, single-factor analysis of variance). The smallest muscularized vessel was 113 +/- 50 microm, and the largest nonmuscularized vessel was 138 +/- 49 microm in lambs with CDH, significantly different from control lambs (185 +/- 69 microm and 350 +/- 116 microm, respectively) and lambs with CDH + TO (185 +/- 97 microm and 245 +/- 100 microm, respectively; P <.05). In lambs with CDH, only 25% of vessels of less than 60 microm were nonmuscularized, compared with 81% in control lambs (P <.05) and 74% in lambs with CDH + TO.Conclusions. Temporary tracheal occlusion, from 108 to 122 days, corrects the abnormal muscularization of pulmonary arterioles seen in CDH. These morphometric findings parallel physiologic results at birth and further suggest that short-term occlusion, which preserves surfactant-producing type II pneumocytes without lung growth, may be sufficient to improve neonatal outcome of diaphragmatic hernia.
Division of Pediatric Surgery and Department of Pathology, Brown University School of Medicine, Providence, RI 02905, USA.
F I
FI
Luks
Y K
YK
Wild
G J
GJ
Piasecki
M E
ME
De Paepe
D000199:Actins / Q000032:analysis; D000818:Animals; D001160:Arterioles / Q000196:embryology / Q000502:physiology; D001835:Body Weight; D004549:Elastin / Q000032:analysis; D005260:Female; D006548:Hernia, Diaphragmatic / Q000196:embryology* / Q000601:surgery; D006976:Hypertension, Pulmonary / Q000517:prevention & control*; D008168:Lung / Q000098:blood supply* / Q000196:embryology*; D009929:Organ Size; D011247:Pregnancy; D011652:Pulmonary Circulation / Q000502:physiology*; D012756:Sheep; D014132:Trachea / Q000196:embryology* / Q000601:surgery*
D000199:Actins; D004549:Elastin