The monoclonal antibody MAC252 does not react with the (-) enantiomer of abscisic acid.
An RIA procedure has been developed for ABA quantification using MAC62, a monoclonal antibody raised against (+)-cis, trans -ABA. This widely used method now relies on MAC252, a recloned version of the exhausted MAC62. Recently, it has been suggested that MAC252 was not able to discriminate between the (+) and (-) enantiomers of ABA. As this can be misleading when interpreting RIA results, it has been carefully examined here whether MAC252 reacts with (-)-ABA. MAC252 exhibited negligible cross-reactivity with (-)-ABA, which was confirmed with commercial mixtures of ABA isomers. It is concluded that the RIA protocol can continue to be used with MAC252 as it was with MAC62.
Barrieu
P
P
INRA, Montepellier, France.
Simonneau
T
T
D000040:Abscisic Acid / Q000737:chemistry / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D011863:Radioimmunoassay; D013237:Stereoisomerism
D000911:Antibodies, Monoclonal; D000040:Abscisic Acid