Immunocytochemical study of malignant lymphoma in serous effusions.
OBJECTIVE
To cytologically evaluate a large series of serous effusions associated with malignant lymphoma (ML), identify the immunoreaction patterns of the cells from selected positive cases and to investigate the correlation of cytomorphology with tissue section diagnosis.


METHODS
From 1966 to 1990, a review of the files of the Department of Anatomic Pathology, A. C. Camargo Hospital, disclosed 4,297 cases of serous effusions, 256 of which were associated with ML. Cytopathologically positive cases were selected for immunocytochemical study. All paraffin sections stained with hematoxylin and eosin were reviewed to confirm the malignancy of the cases. Immunostaining was performed on both cytocentrifuge slides previously stained with Papanicolaou stain and new sections of the biopsy samples using the immunoperoxidase method and avidin-biotin complex with monoclonal mouse antihuman B-cell marker L-26 and monoclonal (mouse) antihuman T-cell marker UCHL-1.


RESULTS
Immunocytochemical reactions were performed in 54 cases: 22 were pan-B positive and 10 pan-T positive; 24 cases showed no reactivity for either monoclonal antibody. Immunohistochemical reactions were performed in 24 available cases: 15 were pan-B positive and pan-T positive; 3 cases showed no reactivity for either monoclonal antibody. Cytohistoimmunoreactions were similar in 11 pan-B positive cases and 2 pan-T positive cases. Three cases were negative for both markers, 4 cases were pan-T positive in tissue samples and negative in cytocentrifuge smears, 3 cases were pan-B positive in tissue and negative on cytology and 3 cases were negative for both markers in both tissue and cytologic specimens.


CONCLUSIONS
Cytology is an effective method of evaluating serous effusions associated with malignant lymphoma: no false positive diagnosis was observed in this series. Immunophenotyping of lymphoid cells is useful to classify and confirm the cytologic diagnosis.
Department of Pathology, Medicine School, Federal University of São Paulo State, Brazil.
G C
GC
Santos
A
A
Longatto-Filho
L V
LV
de Carvalho
J I
JI
Neves
A C
AC
Alves
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000276:immunology / Q000473:pathology*; D051379:Mice; D012704:Serous Membrane / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology