A new assay for lipiodol in a tumor using a combination of m-chloroperbenzoic acid-mediated oxidation and the iodo-starch reaction.
Lipiodol, an iodine adduct lipid, has been used as a targeting carrier of anticancer drugs in experimental animals and humans. In most studies, the concentrations of the anticancer drugs in tissues and organs have been monitored, but not of the carrier because a simple method for measuring lipiodol in biological organs did not exist. Here we present an analytical method for the quantitative determination of lipiodol in tissue. This method is based on the measurement of iodine released from lipiodol by an oxidative reaction. The released iodine was measured spectrophotometrically by monitoring the iodo-starch reaction. Using this method, we were able to demonstrate the tumor specificity of lipiodol using rabbits bearing VX2 tumors in the liver. The present method is also expected to be applicable to human cancers, such as hepatic and colon cancer.
Kozutsumi
D
D
Pharmaceuticals Development Department, Meiji Milk Products Co., Ltd. Naruda 540, Odawara, 250-0862, Kanagawa, Japan. daisuke_kozutsumi@meiji-milk.com
Ohshiba
Y
Y
Sugimoto
T
T
Tsuboi
H
H
Kawashima
A
A
Kochiya
M
M
Adachi
M
M
Takami
M
M
D000818:Animals; D002723:Chlorobenzoates / Q000737:chemistry*; D006801:Humans; D007455:Iodine / Q000737:chemistry; D007459:Iodized Oil / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D008297:Male; D009369:Neoplasms / Q000737:chemistry*; D011786:Quality Control; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D013213:Starch / Q000737:chemistry
D002723:Chlorobenzoates; D007459:Iodized Oil; D013213:Starch; D007455:Iodine; C000433:3-chloroperbenzoic acid