[Segmented condensation of peptides on a solid phase using subtilisin complexed with sodium dodecyl sulfate].
The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the coupling of peptide segments on a solid phase in organic medium. By a two-stage enzymic condensation of peptide fragments on aminosilochrom (A) containing Met-Ala-Gly as a spacer, Dnp(or Boc)-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A and Z-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A were obtained. It was shown that the condensation products can be split off from the support using the Met residue cleavage by BrCN.
Lomonosov State University, Chemical Department, Vorob'evy gory, Moscow, Russia.
S V
SV
Kolobanova
I Iu
IIu
Filippova
E N
EN
Lysogorskaia
I V
IV
Getun
A V
AV
Bacheva
V M
VM
Stepanov
D010455:Peptides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013381:Subtilisins / Q000737:chemistry*
D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D013381:Subtilisins