A structural model of transcription elongation.
The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by the beta' subunit and enclosed on top by the beta subunit. In the RNAP channel, the RNA/DNA hybrid extends from the enzyme active site, along a region of the beta subunit harboring rifampicin resistance mutations, to the beta' subunit "rudder." The single-stranded RNA is then extruded through another channel formed by the beta-subunit flap domain. The model provides insight into the functional properties of the transcription complex.
Korzheva
N
N
Public Health Research Institute, 455 First Avenue, New York, NY 10016, USA.
Mustaev
A
A
Kozlov
M
M
Malhotra
A
A
Nikiforov
V
V
Goldfarb
A
A
Darst
S A
SA
D001665:Binding Sites; D003432:Cross-Linking Reagents; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013698:Templates, Genetic; D013824:Thermus / Q000201:enzymology; D014158:Transcription, Genetic
D003432:Cross-Linking Reagents; D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D004247:DNA; D012321:DNA-Directed RNA Polymerases