Cloning and characterization of PKC-homologous genes in the truffle species Tuber borchii and Tuber magnatum.
The protein kinases C (PKCs) define a growing family of ubiquitous signal transducting serine/threonine kinases that control ion conductance channels, release of hormones and cell growth and proliferation. Degenerated oligonucleotides were used as primers for polymerase chain reactions to amplify PKC-related sequences from the white truffle species Tuber magnatum and Tuber borchii. The deduced amino acid sequences of cloned sequences reveal domains homologous to the regulatory and kinase domains of PKC-related proteins, but lack typical Ca(2+)-binding domain and therefore should be classified as nPKCs. Both contain a large extended N-terminus which is found exclusively in fungi PKCs. Phylogenetic analysis of the kinase domain demonstrates high homology with known filamentous fungi isoenzymes.
Department of Cellular Biotechnology and Hematology, Section of Molecular Genetics, University La Sapienza, Polyclinic Umberto I, Viale Regina Elena 324, I-00161, Rome, Italy.
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D000595:Amino Acid Sequence; D001203:Ascomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011493:Protein Kinase C / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D011493:Protein Kinase C