Halocin S8: a 36-amino-acid microhalocin from the haloarchaeal strain S8a.
Halocin S8 is a hydrophobic microhalocin of 36 amino acids (3,580 Da) and is the first microhalocin to be described. This peptide antibiotic is unique since it is processed from inside a much larger, 33,962-Da pro-protein. Halocin S8 is quite robust, as it can be desalted, boiled, subjected to organic solvents, and stored at 4 degrees C for extended periods without losing activity. The complete amino acid sequence of halocin S8 was obtained first by Edman degradation of the purified protein and verified from the halS8 gene: H(2)N-S-D-C-N-I-N-S-N-T-A-A-D-V-I-L-C-F-N-Q-V-G-S-C-A-L-C-S-P-T-L-V-G -G-P-V-P-COOH. The halS8 gene is encoded on an approximately 200-kbp megaplasmid and contains a 933-bp open reading frame, of which 108 bp are occupied by halocin S8. Both the halS8 promoter and the "leaderless" halS8 transcript are typically haloarchaeal. Northern blot analysis revealed three halS8 transcripts: two abundant and one minor. Inspection of the 3' end of the gene showed only a single, weak termination site (5'-TTTAT-3'), suggesting that some processing of the larger transcripts may be involved. Expression of the halS8 gene is growth stage dependent: basal halS8 transcript levels are present in low concentrations during exponential growth but increase ninefold during the transition to stationary phase. Initially, halocin activity parallels halS8 transcript levels very closely. However, when halocin activity plateaus, transcripts remain abundant, suggesting inhibition of translation at this point. Once the culture enters stationary phase, transcripts rapidly return to basal levels.
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Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640, USA.
Shand
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D000595:Amino Acid Sequence; D000596:Amino Acids; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides; D019843:Archaeal Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D019641:DNA, Archaeal; D015870:Gene Expression; D020869:Gene Expression Profiling; D019766:Genes, Archaeal; D005800:Genes, Fungal; D016681:Genome, Fungal; D006216:Halobacteriaceae / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides; D012331:RNA, Fungal; D013728:Terminator Regions, Genetic
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D019843:Archaeal Proteins; D019641:DNA, Archaeal; D010455:Peptides; D012331:RNA, Fungal; C413338:halocin S8