Cellular immune response of intracecally inoculated Mongolian gerbils with Entamoeba histolytica trophozoites.
We analyzed the local and systemic cellular immune response in mongolian gerbils inoculated with Entamoeba histolytica. Two groups were intracecally inoculated with E. histolytica trophozoites and two groups were used as controls. A inoculated groups and a control groups were sacrificed on days 10 and 30 post inoculation (p.i), the spleen and mesenteric lymph nodes (MLNs) lymphocytes (Ly) were isolated and incubated in vitro with 2 different amebic antigens. The proliferative Ly response of inoculated groups was greater than the Ly mitogenic response seen in control groups, at day 10 p.i. as well as day 30 p.i. (alpha = 0.05). Ly response of MLNs was greater in comparison to those of the spleen (alpha = 0.05). In other four groups, intradermal reactions with a antigen were used to demonstrated delayed hypersensitivity in gerbils after being inoculated with E. histolytica trophozoites. The percentage of volume increase of the plantar pad swelling were measured. Groups inoculated presented greater increases (alpha = 0.05) than groups controls. The evidence presented herein demonstrates that the presence of E. histolytica trophozoites in cecum induced a local and systemic cellular immune response.
Alba-Hurtado
F
F
Departamento de Ciencias Biológicas, Facultad de Estudios Superiores, Cuautitlán, Universidad Nacional Autónoma de México.
Díaz-Otero
F
F
Valdivia
G
G
Reyes
R
R
Tsutsumi
V
V
Acosta
G
G
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D002432:Cecum / Q000276:immunology*; D004748:Entamoeba histolytica / Q000254:growth & development / Q000276:immunology*; D004749:Entamoebiasis / Q000276:immunology*; D005849:Gerbillinae / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007111:Immunity, Cellular; D007413:Intestinal Mucosa / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D012882:Skin Tests; D013154:Spleen / Q000276:immunology
D000953:Antigens, Protozoan