Random amplified polymorphic DNA (RAPD) analysis of Paracoccidioides brasiliensis isolates.
Five Paracoccidioides brasiliensis isolates of humans origin were analyzed using three arbitrary primers (3301, 3304 and 3307 of 10, 9 and 10 oligonucleotídes respectively) in random amplified polymorphic DNA (RAPD) analysis. The analysis of the complex RAPD profiles obtained were carried out using the Dice similarity coefficient that distinguished the isolated Pb 02 from the others (Pb 18, Pb 192, Pb 265 and Pb SN). The results revealed limited intraspecific genomic variations in these P. brasiliensis isolates and indicate that RAPD can be useful for analysis of P. brasiliensis genome for characterization or differentiation within this genus.
Totti
D O
DO
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Romanha
A J
AJ
Grisard
E C
EC
Simpson
A J
AJ
Koury
M C
MC
D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D016681:Genome, Fungal; D006801:Humans; D010228:Paracoccidioides / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010229:Paracoccidioidomycosis / Q000382:microbiology*; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
D004271:DNA, Fungal