Prevention of systemic lupus erythematosus-like disease in (NZBxNZW)F1 mice by treating with CDR1- and CDR3-based peptides of a pathogenic autoantibody.
Two peptides based on the complementarity-determining regions (CDR) of a pathogenic murine anti-DNA antibody were employed in an attempt to prevent the spontaneous systemic lupus erythematosus (SLE)-like disease of (NZBxNZW)F1 mice. Female mice, at the age of 2 months, were injected with either the CDR1- or the CDR3-based peptides (pCDR1, pCDR3) subcutaneously or intravenously in aqueous solution for a total of 8-10 treatments. A reduction was observed in the total and pathogenic IgG2a and IgG3 anti-DNA antibody titers in the CDR-treated groups. Treatment reduced the number of mice that developed proteinuria and immune complex deposits in their kidneys. The severity of renal pathology was significantly reduced in the pCDR3 (P<0.02) and pCDR1 (P< or = 0.05) treated mice. Thus, both CDR-based peptides administered in aqueous solution were capable of preventing the SLE-like disease in (NZBxNZW)F1 mice, although the beneficial effects of pCDR3 appeared to be more pronounced than those of pCDR1 in the treated mice.
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
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Eilat
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Zinger
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Nyska
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Mozes
D000595:Amino Acid Sequence; D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005260:Female; D007116:Immunization, Passive; D007128:Immunoglobulin Fragments / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D007074:Immunoglobulin G / Q000097:blood; D007167:Immunotherapy; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007668:Kidney / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000517:prevention & control*; D008181:Lupus Nephritis / Q000473:pathology / Q000517:prevention & control; D051379:Mice; D008814:Mice, Inbred NZB; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G