Induced expression of sarcotoxin IA enhanced host resistance against both bacterial and fungal pathogens in transgenic tobacco.
We demonstrate here that induced expression of sarcotoxin IA, a bactericidal peptide from Sarcophaga peregrina, enhanced the resistance of transgenic tobacco plants to both bacterial and fungal pathogens. The peptide was produced with a modified PR1a promoter, which is further activated by salicylic acid treatment and necrotic lesion formation by pathogen infection. Host resistance to infection of bacteria Erwinia carotovora subsp. carotovora and Pseudomonas syringae pv. tabaci was shown to be dependent on the amounts of sarcotoxin IA expressed. Since we found antifungal activity of the peptide in vitro, transgenic seedlings were also inoculated with fungal pathogens Rhizoctonia solani and Pythium aphanidermatum. Transgenic plants expressing higher levels of sarcotoxin were able to withstand fungal infection and remained healthy even after 4 weeks, while control plants were dead by fungal infection after 2 weeks.
National Institute of Agrobiological Resources, Tsukuba City, Ibaraki, Japan.
I
I
Mitsuhara
H
H
Matsufuru
M
M
Ohshima
H
H
Kaku
Y
Y
Nakajima
N
N
Murai
S
S
Natori
Y
Y
Ohashi
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001419:Bacteria / Q000472:pathogenicity*; D001483:Base Sequence; D017931:DNA Primers; D005658:Fungi / Q000472:pathogenicity*; D019476:Insect Proteins / Q000502:physiology*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D014026:Nicotiana / Q000276:immunology* / Q000382:microbiology
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D017931:DNA Primers; D019476:Insect Proteins; C054733:sarcotoxin IA protein, Sarcophaga peregrina