Preparative isolation and purification of notopterol and isoimperatorin from Notopterygium forbessi Boiss (Chinese traditional medicinal herb) by high-speed counter-current chromatography.
Preparative high-speed counter-current chromatography was successfully used for isolation and purification of coumarins from Chinese traditional medicinal herb Notopterygium forbessi Boiss (Qianghuo in Chinese) using stepwise elution with a pair of two-phase solvent systems composed of light petroleum-ethyl acetate-methanol-water at volume ratios of 5:5:4.8:5 and 5:5:5:4. Four major components including notopterol and isoimperatorin were isolated, each at over 98% purity.
Yang
F
F
Beijing Institute of New Technology Application, China.
Zhang
T
T
Liu
Q
Q
Xu
G
G
Zhang
Y
Y
Zhang
S
S
Ito
Y
Y
D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D011564:Furocoumarins / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; C055542:isoimperatorin; C082412:notopterol