Cometabolic biotransformation of nitrobenzene by 3-nitrophenol degrading Pseudomonas putida 2NP8.
A strain of Pseudomonas putida (2NP8) capable of growing on both 2-nitrophenol and 3-nitrophenol, but not on nitrobenzene (NB), was isolated from municipal activated sludge. 2-Nitrophenol was degraded by this strain with production of nitrite. Degradation of 3-nitrophenol resulted in the formation of ammonia. Cells grown on 2-nitrophenol did not degrade nitrobenzene. A specific nitrobenzene degradation activity was induced by 3-nitrophenol. Ammonia, nitrosobenzene, and hydroxylaminobenzene have been detected as metabolites of nitrobenzene degradation by cells grown in the presence of 3-nitrophenol. These results indicated a NB cometabolism mediated by 3-nitrophenol nitroreductase.
Department of Biology, University of Waterloo, ON, Canada.
J S
JS
Zhao
O P
OP
Ward
D001673:Biodegradation, Environmental; D004783:Environmental Microbiology; D009578:Nitrobenzenes / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism*; D016958:Pseudomonas putida / Q000302:isolation & purification / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology
D009578:Nitrobenzenes; D009596:Nitrophenols; D012722:Sewage; C049017:3-nitrophenol