Modulation of actA gene expression in Listeria monocytogenes by iron.
This study analysed the invasiveness of Listeria monocytogenes into enterocyte-like Caco-2 cells in which iron depletion was achieved by picolinic acid treatment. Both entry and intracellular multiplication varied depending on the endogenous iron content of bacterial and eukaryotic cells. The behaviour within enterocytes was correlated with a 10-fold increased transcription of the actA gene observed in bacterial cells grown under conditions of iron stress.
Maria Pia
MP
Conte
C
C
Longhi
G
G
Petrone
M
M
Polidoro
Institute of Microbiology, University of Rome 'La Sapienza', Rome and *Institute of Microbiology, II University of Naples, Naples, Italy.
P
P
Valenti
L
L
Seganti
D001426:Bacterial Proteins / Q000235:genetics*; D018938:Caco-2 Cells; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007501:Iron / Q000502:physiology*; D008089:Listeria monocytogenes / Q000235:genetics* / Q000472:pathogenicity; D008565:Membrane Proteins / Q000235:genetics*; D014774:Virulence
D001426:Bacterial Proteins; D008565:Membrane Proteins; C072980:actA protein, Listeria monocytogenes; D007501:Iron