Expression and localization of utrophin in differentiating PC12 cells.
To ascertain the role of utrophin in cultured neuronal cells, we investigated its expression and distribution along the NGF-induced differentiation of PC12 cells grown on different substrata. Utrophin mRNA was measured by RT-PCR assay and utrophin protein was quantified by immunoblot analysis. The distribution of utrophin and beta-dystroglycan was analyzed by confocal microscopy. We demonstrate that utrophin protein was increased 4-fold during differentiation of cells grown laminin. Concomitant with this up-regulation, utrophin was enriched at the growth cones in differentiating cells, where it co-localizes with beta-dystroglycan. These data suggest the presence of a utrophin-beta-dystroglycan complex in PC12 cells that participates in the formation and/or stabilization of the growth cone-extracellular matrix adhesion.
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Avenida Instituto Politécnico Nacional 2508, México DF.
H
H
Rosas-Vargas
C
C
Montañez
A
A
Rendón
D
D
Mornet
F
F
García
V
V
Ceja
B
B
Cisneros
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics*; D049030:Dystroglycans; D005786:Gene Expression Regulation / Q000187:drug effects; D007797:Laminin; D008562:Membrane Glycoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D018613:Microscopy, Confocal; D020932:Nerve Growth Factor / Q000494:pharmacology; D016716:PC12 Cells; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017474:Receptors, Laminin / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D049411:Utrophin
D003598:Cytoskeletal Proteins; D007797:Laminin; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D017474:Receptors, Laminin; D049411:Utrophin; D049030:Dystroglycans; D020932:Nerve Growth Factor