Decreased CD10-positive mature granulocytes in bone marrow from patients with myelodysplastic syndrome.
OBJECTIVE
We retrospectively examined the maturation of the granulocytic cell lineage in bone marrow specimens from patients with myelodysplastic syndrome (MDS) by flow cytometry using both light scatter and surface marker characteristics, including CD10 and myeloid lineage-associated antigens.


METHODS
The 7 MDS cases we studied included 2 patients with refractory anemia (RA), 3 with RA with ringed sideroblasts, 1 with RA with excess of blasts, and 1 unclassified case. Another 7 patients matched for age and sex who received bone marrow aspirates for lymphoma staging (all negative for lymphoma involvement or any other hematologic abnormalities) were selected as the control group.


RESULTS
The percentage of CD10(+) mature granulocytes was significantly lower in patients with MDS than in control patients. Additionally, all patients with MDS had less than 50% CD10(+) cells in the granulocytic lineage. In contrast, only 1 of the 7 control patients had less than 50% CD10(+) cells (P <.01).


CONCLUSIONS
These results suggest that flow cytometry might be a useful adjunct in the assessment of patients with suspected MDS. Further studies to correlate CD10(+) mature granulocytes from MDS cases with other benign hematologic disorders are indicated to confirm our evaluation.
Department of Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.
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Chang
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Cleveland
D000368:Aged; D000369:Aged, 80 and over; D000943:Antigens, Differentiation / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology* / Q000473:pathology; D001856:Bone Marrow Examination; D019070:Cell Lineage; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology / Q000378:metabolism*; D015798:HLA-DR1 Antigen / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D015260:Neprilysin / Q000378:metabolism*; D011379:Prognosis; D012189:Retrospective Studies
D000943:Antigens, Differentiation; D015798:HLA-DR1 Antigen; D015260:Neprilysin