Giant vesicles as models to study the interactions between membranes and proteins.
The interaction between polypeptides and membranes is a fundamental aspect of cell biochemistry. Liposomes have been used in this context as in vitro systems to study such interactions. We present here the case of giant vesicles (GVs), which, due to their size (radius larger than 10 microns), mimic more closely the situation observed in cell membranes and furthermore permit to study protein-membrane interactions by direct optical monitoring. It is shown that GVs formed from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine by electroformation are permeable to certain low molecular weight molecules such as the nucleic acid dye YO-PRO-1 and fluorescein diphosphate whereas conventional liposomes (large or small unilamellar liposomes) are not. In addition, it is shown that non-membrane proteins, such as DNases or RNases, added to the selected GVs from the outside, are able to convert their substrate, which is strictly localized on the internal side of the membrane. This effect is only seen in GVs (also when they are removed from the original electroformation environment) and is absent in conventional liposomes. The fact that these effects are only present in GVs obtained by electroformation and not in conventional small liposomes is taken as an indication that certain physico-chemical properties of the bilayer are affected by the membrane curvature, although the mechanism underlying such differences could not be established as yet.
Fischer
A
A
Institut für Polymere, ETH Zentrum, Zürich, Switzerland.
Oberholzer
T
T
Luisi
P L
PL
D002462:Cell Membrane / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005573:Formycins / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D009930:Organic Chemicals; D010316:Particle Size; D010539:Permeability; D010713:Phosphatidylcholines; D011506:Proteins / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry; D012265:Ribonucleotides / Q000737:chemistry
D005456:Fluorescent Dyes; D005573:Formycins; D008081:Liposomes; D009930:Organic Chemicals; D010713:Phosphatidylcholines; D011506:Proteins; D012265:Ribonucleotides; C405903:TO-PRO-1; C020332:formycin diphosphate; D003851:Deoxyribonucleases; D012260:Ribonucleases; C028694:1-palmitoyl-2-oleoylphosphatidylcholine