Development of surgical confocal scanning microscope for intra-operative imaging of brain tumors using near infrared fluorescence: technical note.
We developed a confocal laser scanning microscope for intra-operative imaging of brain tumors using near infrared fluorescence. The quality of near infrared images of Indocyanine Green (ICG) was compared with the surgical confocal scanning (SCS) microscope and a conventional charge-coupled device (CCD) camera; we compared images of a tube filled with ICG, which was located in the mouse brain. Compared to the CCD camera, the SCS microscope could obtain a more precise image of ICG fluorescence through the brain tissue. In addition, the SCS microscope could image ICG fluorescence clearly in a relatively light room because of elimination of stray light, while the CCD camera required high darkness to obtain ICG images. The present SCS microscope can give useful intra-operative imaging of brain tumors, particularly detection of residual tumor tissues that extend into normal brain tissues.
Sakatani
K
K
Department of Neurosurgery, China-Japan Friendship Hospital, Beijing, China. sakatani@public.east.cn.net
Kashiwasake-Jibu
M
M
Terada
H
H
Zuo
H
H
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D002102:Cadaver; D004396:Coloring Agents; D005453:Fluorescence; D007208:Indocyanine Green; D007259:Infrared Rays; D007432:Intraoperative Period; D051379:Mice; D018613:Microscopy, Confocal
D004396:Coloring Agents; D007208:Indocyanine Green