Cloning and characterization of the mouse tob2 gene.
Human Tob2 is a member of the Tob/BTG1 anti-proliferative family of proteins. Here, we report the molecular cloning and characterization of the mouse tob2 gene. The tob2 gene contains an open reading frame of 345 amino acids with an 89% identity to its human counterparts. The coding region of mouse tob2 is not interrupted by introns. The tob2 transcript is 4.2kb long, the size being similar to that of the human tob2 transcript, and detected ubiquitously in various tissues of adult mice. In addition, in situ hybridization shows that tob2 is ubiquitously expressed in embryo, the level of expression being especially high in skeletal muscle. Collectively, Tob2 is suggested to play roles both during embryogenesis and in adults.
Department of Oncology, The Institute of Medical Science, The University of Tokyo, Minato-ku, 108-8639, Tokyo, Japan.
R
R
Ajima
N
N
Ikematsu
M
M
Ohsugi
Y
Y
Yoshida
T
T
Yamamoto
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018797:Cell Cycle Proteins / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018797:Cell Cycle Proteins; D012333:RNA, Messenger; C402648:TOB2 protein, human; C485732:Tob2 protein, mouse; D004247:DNA