UDS induction by an array of standard carcinogens in human and rodent hepatocytes: effect of cryopreservation.
The UDS induction assay with primary hepatocytes as the target cells is a determinative assay for chemical carcinogens. This assay is, however, limited to the availability of freshly prepared liver cells. A cryopreservation technique for liver cells has recently been described. Frozen cells have been shown to retain a variety of enzyme activities essential for xenobiotic metabolism after being thawed. In the present investigation, 19 direct or indirect-acting carcinogens were tested with respect to their capacity to induce DNA repair in primary as well as cryopreserved human and rat hepatocytes. Cryopreserved cells yielded results that were essentially indistinguishable from fresh cells. Only marginal differences were observed between hepatocytes of rat or human origin. These results demonstrate the suitability of cryopreserved hepatocytes as indicator cells for the study of UDS induction to discover possible carcinogenicity in chemicals.
Department of Toxicology, University Medical School Hamburg, Grindelallee 117, 20146, Hamburg, Germany.
L
L
Schehrer
J D
JD
Regan
J
J
Westendorf
D000328:Adult; D000368:Aged; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D004260:DNA Repair; D005260:Female; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009152:Mutagenicity Tests / Q000379:methods; D051381:Rats; D017208:Rats, Wistar
D002273:Carcinogens; D004247:DNA