Influence of antigenic forms and adjuvants on protection against a lethal infection of Aujeszky's disease virus.
The influence of antigenic forms and adjuvant types on protection against a lethal infection of Aujeszky's disease virus (ADV) in mice was investigated. Antiviral IgG2a antibody response against particulate (inactivated ADV) and soluble antigen (ADV solubilized with deoxychorate-Na) in approximate order of extent was ISA70>QS-21>positively charged liposome>negatively charged liposome>weak negatively charged liposome>ISA25>lablabside F saponin>aluminum phosphate gel>non adjuvant. Particulate antigen induced higher IgG2a antibody production than soluble antigen. Particulate antigen combined with ISA70, ISA25 or positively charged liposome gave 100, 50 and 40% protection to mice, respectively. In contrast, soluble antigen plus ISA70 conferred 30% protection on mice. Immunogens using the other adjuvants gave </=20% protection to mice. These results indicate that a combination of particulate antigen and an appropriate adjuvant effectively induces the production of antiviral IgG2a antibody and provides protection against a lethal ADV infection in mice.
Division of Veterinary Microbiology, Kyoto Biken Laboratories, 24-16 Makishima-cho, Kyoto 611-0041, Uji, Japan.
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Katayama
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Oda
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Ohgitani
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage*; D004195:Disease Models, Animal; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D016896:Treatment Outcome
D000276:Adjuvants, Immunologic; D000956:Antigens, Viral