Changes in the composition of circulating CD8+ T cell subsets during acute epstein-barr and human immunodeficiency virus infections in humans.
In response to viral infection, unprimed naive CD8(+), major histocompatibility complex class I-restricted, virus-specific T cells clonally expand and differentiate into memory- and effector-type cells. Changes in CD8(+) subset distribution were studied in 17 subjects with acute human immunodeficiency virus type 1 infection and in 14 subjects with acute Epstein-Barr virus (EBV) infection, with combined CD45RO, CD27, and CD28 monoclonal antibodies. A vast expansion of memory-type CD45RO(+)CD27(+)CD8(+) T cells, with high expression of the cell-cycle marker Ki-67, was observed in both infections. Strikingly, CD45RO(+)CD27(+)CD28(-) cells increased >10-fold in acute viral infection and had high Ki-67 expression. In acute EBV infection, a substantial portion of the expanded T cells were EBV-peptide specific. These cells resided mainly in the CD45RO(+)CD27(+) subpopulation, with most in the CD27(+)CD28(-) subpopulation. Content of perforin expression, as a measure of cytotoxic capacity, was relatively low in the CD27(+)CD28(+) T cells and highest in the CD27(-)CD28(-) subpopulation.
Roos
M T
MT
Department of Clinical Viro-Immunology, CLB and Laboratory for Experimental and Clinical Immunology of the University of Amsterdam at the CLB, Amsterdam, The Netherlands. m_roos@clb.nl
van Lier
R A
RA
Hamann
D
D
Knol
G J
GJ
Verhoofstad
I
I
van Baarle
D
D
Miedema
F
F
Schellekens
P T
PT
D018106:CD28 Antigens; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D020031:Epstein-Barr Virus Infections / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015795:HLA-B8 Antigen; D006801:Humans; D019394:Ki-67 Antigen / Q000302:isolation & purification; D017493:Leukocyte Common Antigens; D008562:Membrane Glycoproteins / Q000302:isolation & purification; D010446:Peptide Fragments; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7
D018106:CD28 Antigens; D015795:HLA-B8 Antigen; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D052899:Pore Forming Cytotoxic Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D054353:Perforin; D017493:Leukocyte Common Antigens; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1