Galectin-3 decreases in mice exposed to immobilization stress.
Using M3/38 monoclonal antibody we have analyzed effects of immobilization stress on the expression of galectin-3 in liver, spleen and peritoneal macrophages from adult RFM and C3H mice, as well as from aged C3H mice (total of 22 animals). In all analyzed tissues, immobilization stress caused a significant decrease in the expression of galectin-3, ranging from 14% to 47%. The decrease of galectin-3 was observed in both strains of mice, as well as in old animals. Moreover, the same range of decrease (approximately 50%) was observed when cells grown in vitro were exposed to subculturing, or heat-shock. Although the biological significance of this effect is not known, it is very interesting that a single episode of immobilization stress was sufficient to cause a significant decrease in galectin-3, implicating that this beta-galactoside-binding lectin might be involved in the physiological response to psychological stress.
Dumić
J
J
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovacića 1, 10000 Zagreb, Croatia.
Barisić
K
K
Flögel
M
M
Lauc
G
G
D000375:Aging / Q000502:physiology*; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism*; D002460:Cell Line; D037502:Galectin 3; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D018869:Heat-Shock Response / Q000502:physiology; D015151:Immunoblotting; D008099:Liver / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D012149:Restraint, Physical; D013154:Spleen / Q000378:metabolism; D013312:Stress, Physiological / Q000378:metabolism*
D000943:Antigens, Differentiation; D037502:Galectin 3