Gelatinase A in human dentin as a new biochemical marker for age estimation.
Dentin proteins from 24-individual permanent molars from patients aged 15 to 73 years were sequentially extracted, with guanidinium chloride (G1-extract) and then with EDTA; after demineralization, the material was again extracted with guanidinium chloride (G2-extract). Extracts were analyzed by SDS-PAGE electrophoresis and the gels were processed for zymography to detect gelatinolytic activities. The patterns of gelatinase A distribution differed in the different dentin protein fractions, and the changes varied with age. Significant differences were detected in gelatinase A in G2-extracts between individual younger than 20 years old and the rest of the sample (chi2exp = 19.429; 1 d.f.; p < or = 0.001). The incidence of true and false positives and negatives, and sensitivity and specificity for the presence of gelatinase A in dentin extracts, were calculated. Determination of gelatinase A in human dentin may be a useful marker to estimate age, especially when other morphological methods are of limited usefulness.
Department of Forensic Medicine and Forensic Odontology, University of Granada, Spain.
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Martín-de las Heras
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Valenzuela
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Overall
D000293:Adolescent; D000328:Adult; D000366:Age Determination by Teeth / Q000379:methods*; D000368:Aged; D003804:Dentin / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D008875:Middle Aged; D012680:Sensitivity and Specificity
D020778:Matrix Metalloproteinase 2