Replication origin of Streptococcus pyogenes, organization and cloning in heterologous systems.
The origin of DNA replication (oriC) of Streptococcus pyogenes, group A streptococci (GAS), has been cloned in Escherichia coli and reintroduced by transformation into other GAS strains. Transformation frequencies into GAS strains with oriC-carrying plasmids occurred with unusually high frequencies. However, the oriC-containing plasmids in the new recipients were found to be unstable and had a tendency to integrate into the chromosome, even when a recA GAS strain was used as a recipient. The GAS oriC was able to direct the replication of autonomous plasmids in group B streptococcal recipients. The chromosomal organization of the oriC region of GAS relative to other bacterial species appears to be similar to oriC of Bacillus subtilis and other Gram-positive microorganisms.
Institute of Experimental Medicine, Academy of Medical Sciences, St. Petersburg, Russia. lab@suvorov.spb.su
A N
AN
Suvorov
J J
JJ
Ferretti
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D018741:Replication Origin / Q000235:genetics*; D013297:Streptococcus pyogenes / Q000235:genetics*