Biocatalysis for pharmaceuticals--status and prospects for a key technology.
In reviewing how biocatalysis can be applied to improve chiral synthesis for pharmaceuticals it becomes clear that there will be many opportunities using a simple enzyme system but that many of the more useful applications will require the whole cell because of the requirement for cofactors. An assessment is made of the opportunities to apply metabolic engineering to construct de novo metabolic pathways for the biosynthesis of useful advanced intermediates and a conceptual example is provided for the biosynthesis of cis-aminoindanol. We predict that in the future novel pathways will be assembled for a one-step biosynthesis of many semisynthetic natural products.
Bioprocess R&D, Merck Research Laboratories, Rahway, New Jersey 07065-0900, USA.
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BC
Buckland
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DK
Robinson
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Chartrain
D000818:Animals; D002384:Catalysis; D015195:Drug Design; D004798:Enzymes / Q000378:metabolism; D006801:Humans
D004798:Enzymes