The monoclonal antibody MAC252 does not react with the (-) enantiomer of abscisic acid.
An RIA procedure has been developed for ABA quantification using MAC62, a monoclonal antibody raised against (+)-cis, trans -ABA. This widely used method now relies on MAC252, a recloned version of the exhausted MAC62. Recently, it has been suggested that MAC252 was not able to discriminate between the (+) and (-) enantiomers of ABA. As this can be misleading when interpreting RIA results, it has been carefully examined here whether MAC252 reacts with (-)-ABA. MAC252 exhibited negligible cross-reactivity with (-)-ABA, which was confirmed with commercial mixtures of ABA isomers. It is concluded that the RIA protocol can continue to be used with MAC252 as it was with MAC62.
INRA, Montepellier, France.
P
P
Barrieu
T
T
Simonneau
D000040:Abscisic Acid / Q000737:chemistry / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D011863:Radioimmunoassay; D013237:Stereoisomerism
D000911:Antibodies, Monoclonal; D000040:Abscisic Acid