Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phythphthora parasitica var. nicotianae.
PEG-mediated transformation of protoplasts in the presence of lipofectin was achieved in Phytophthora parasitica var. nicotianae, an oomycete pathogen of tobacco. Using oomycete promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) was introduced into the microorganism. The data show for the first time that this eukaryotic gene reporter can be used in an oomycete, both as a quantitative reporter of gene induction and as a vital marker allowing the study of development of Phytophthora in vitro and in the host plant.
Université Paul Sabatier-CNRS, Castanet-Tolosan, France. bottin@cict.fr
A
A
Bottin
L
L
Larche
F
F
Villalba
E
E
Gaulin
M T
MT
Esquerré-Tugayé
M
M
Rickauer
D015415:Biomarkers; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D010838:Phytophthora / Q000235:genetics* / Q000254:growth & development; D010947:Plants, Toxic; D014026:Nicotiana / Q000382:microbiology; D014170:Transformation, Genetic
D015415:Biomarkers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins