ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan.
A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu(1871)-Leu(1872) bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo.
Department of Molecular Membrane Biology, Cancer Research Institute, Kanazawa University, Ishikawa, Japan. kkuno@kenroku.kanazawa-u.ac.jp
K
K
Kuno
Y
Y
Okada
H
H
Kawashima
H
H
Nakamura
M
M
Miyasaka
H
H
Ohno
K
K
Matsushima
D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; D053674:Aggrecans; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002356:Cartilage / Q000737:chemistry*; D002417:Cattle; D002809:Chondroitin Sulfates / Q000378:metabolism; D019483:Disintegrins / Q000235:genetics / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
C508320:Acan protein, mouse; D053674:Aggrecans; D019483:Disintegrins; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D010446:Peptide Fragments; D011509:Proteoglycans; D011993:Recombinant Fusion Proteins; D002809:Chondroitin Sulfates; D051722:ADAM Proteins; D000071097:ADAMTS1 Protein; C486323:Adamts1 protein, mouse; D008666:Metalloendopeptidases