[Effects of protein kinase C on non-heart organ protection of preconditioning].
OBJECTIVE
To investigate the effects of protein kinase C (PKC) on ischemic (hypoxic) preconditioning (PC) in the non-heart organs and tissue.


METHODS
In the models of ischemia/reperfusion of in situ perfused rat small intestine, limb, and in the model of hypoxia/reoxygenation of cultured rat aortic vascular smooth muscle cells (VSMC), the effects of PKC on ischemic (hypoxic) PC were observed.


RESULTS
PKC inhibitor H7 and polymyxin B completely abolished the protective effects of ischemic PC on small intestine and limb subjected to ischemia/reperfusion injury. Hypoxic PC activated PKC in VSMC and potentiated phosphorylation mediated by PKC.


CONCLUSIONS
The activation of PKC is also the important chain of PC on non-heart organs, and its mechanism might involve protein phosphorylation.
Institute of Geriatric Cardiology, PLA General Hospital, Beijing.
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Liu
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Wang
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Wu
D000818:Animals; D002478:Cells, Cultured; D005121:Extremities / Q000098:blood supply; D007421:Intestine, Small / Q000098:blood supply*; D019194:Ischemic Preconditioning; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011493:Protein Kinase C / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*
D011493:Protein Kinase C