Odorant and pheromone binding by aphrodisin, a hamster aphrodisiac protein.
Aphrodisin is a soluble glycoprotein of hamster vaginal discharges, which stimulates male copulatory behavior. Natural aphrodisin was purified and its post-translational modifications characterized by MALDI-MS peptide mapping. To evaluate its ability to bind small volatile ligands, the aphrodisiac protein was expressed in the yeast Pichia pastoris as two major isoforms differing in their glycosylation degree, but close in conformation to the natural protein. Dimeric recombinant aphrodisins were equally able to efficiently bind odors (2-isobutyl-3-methoxypyrazine and methyl thiobutyrate) and a pheromone (dimethyl disulfide), suggesting that they could act as pheromone carriers instead of, or in addition to, direct vomeronasal neuron receptor activators.
Biochimie et Structure des Protéines, INRA UR 477, Jouy-en-Josas Cedex, France.
L
L
Briand
J
J
Huet
V
V
Perez
G
G
Lenoir
C
C
Nespoulous
Y
Y
Boucher
D
D
Trotier
J C
JC
Pernollet
D000818:Animals; D006224:Cricetinae; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D008647:Mesocricetus; D009812:Odorants; D010675:Pheromones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000235:genetics; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012724:Sex Attractants / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014621:Vagina / Q000378:metabolism
D010675:Pheromones; D011506:Proteins; D011994:Recombinant Proteins; D012724:Sex Attractants; C050082:aphrodisin protein, Mesocricetus auratus