[A rapid method for testing the activity of the repair enzyme uracil-DNA-glycosylase].
A rapid and effective method of testing of a repair enzyme, uracil-DNA-glycosylase, was proposed. As a substrate, a deoxyuridine-containing 5'-32P-labeled deoxyoligonucleotide covalently attached to a polystyrene support (Tenta Gel S-NH2) was used. The ammonia cleavage of the apyrimidine site formed in the enzymic reaction followed by the transition of the labeled oligonucleotide fragment from the solid phase into solution allowed the detection of the enzymic activity.
Sud'ina
A E
AE
Chemical Faculty, Moscow State University, Vorob'evy gory, Russia.
Volkov
E M
EM
Oretskaia
T S
TS
Degtiarev
S Kh
SKh
Gonchar
D A
DA
Kubareva
E A
EA
D001483:Base Sequence; D045647:DNA Glycosylases; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D009699:N-Glycosyl Hydrolases / Q000235:genetics / Q000378:metabolism*; D051981:Uracil-DNA Glycosidase
D017931:DNA Primers; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase