[Mechanism of IL-6 secretion in bone marrow cells from multiple myeloma patients].
OBJECTIVE
To explore the mechanism of interleukin-6(IL-6) secretion in bone marrow cells from patients with multiple myeloma(MM).


METHODS
Expression of IL-6 in bone marrow mononuclear cells(BMMNC) from MM patients(n = 16) and controls(n = 19) was detected by using double labelled immunofluorescence.


RESULTS
In MM group, the positive rates of IL-6 expression were 15.4% +/- 6.5% in BMMNC, 7.9% +/- 2.9% in myeloma cells, and 4.5% +/- 1.2% in stromal cells, while in controls the positive rates were 4.6% +/- 2.3% in BMMNC, 2.6% +/- 1.1% in stromal cells and 0.7% +/- 0.1% in plasma cells, respectively. The positive rates in MM group were all significantly higher than those in controls(P < 0.001). The cytoplasmic IgG+ and vWF+ cells percentages in BMMNC were also significantly higher in MM group than those in controls.


CONCLUSIONS
Elevated IL-6 in MM patients was secreted by both myeloma cells and stromal cells, and the mechanism of both autocrine and paracrine may be involved in pathogeneses of multiple myeloma.
Changzheng Hospital, Second Military Medical University, Shanghai.
Z
Z
Liang
J
J
Hou
S
S
Ding
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology* / Q000473:pathology
D015850:Interleukin-6