mRNA expression of proto-oncogenes and platelet-derived growth factor in proliferative vitreoretinal diseases.
OBJECTIVE
To detect the expression of mRNA of protooncogenes ets-1, c-jun, c-fos, and platelet-derived growth factor (PDGF) in proliferative membranes of patients with proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR).


METHODS
cDNA was synthesized from mRNA of proliferative membranes from patients with PDR, PVR, premacular fibrosis (PMF), acute retinal necrosis (ARN), and age-related macular degeneration (ARMD). Ets-1, c-jun, c-fos, PDGF-A, and PDGF-B cDNA were amplified using reverse transcriptase-polymerase chain reaction (PCR).


RESULTS
Proto-oncogene mRNA was highly expressed in membranes from patients with severe PDR, grade D PVR. PDGF mRNA was expressed in almost all samples.


CONCLUSIONS
Our results suggest that not only PDGF mRNA was expressed in the membranes of patients with PDR and PVR, but proto-oncogenes ets-1, c-jun, and c-fos mRNA were also expressed.
Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan.
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Ren
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Fuse
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Abe
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Tamai
D015342:DNA Probes / Q000737:chemistry; D003930:Diabetic Retinopathy / Q000235:genetics* / Q000378:metabolism; D016762:Genes, fos / Q000235:genetics; D016758:Genes, jun / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D008268:Macular Degeneration / Q000235:genetics* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D050783:Proto-Oncogene Proteins c-ets; D011519:Proto-Oncogenes / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; C494041:ETS1 protein, human; D005819:Genetic Markers; C000715470:MAS1 protein, human; D010982:Platelet-Derived Growth Factor; D000090063:Proto-Oncogene Mas; D050784:Proto-Oncogene Protein c-ets-1; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases