A new microsystem for automated electrorotation measurements using laser tweezers.
We have developed a new microsystem for fast, automated studies of reactions and kinetics of single cells with biochemical or pharmacological agents. A cell spins in an external rotating electric field and the frequency dependence characterises the passive dielectric properties of membrane and cytoplasm. We use a planar microelectrode chip with microchannel (easily covered with a removable slip) for the application of frequencies exceeding 250 MHz to determine cytoplasmic properties in low and high conductivity electrolyte solutions. The laser tweezers serve as a bearing system, rotation is induced by microelectrodes and rotation speed is recorded automatically. This opens up new possibilities in biotechnology, e.g. for drug screening as demonstrated by measuring the influence of ionomycin on the passive dielectric properties of T-lymphoma cells. Additionally, a possible infrared-induced long-term cell damage could be observed by electrorotation and is discussed.
Lehrstuhl für Membranphysiologie, Institut für Biologie, Humboldt-Universität zu Berlin, Germany.
C
C
Reichle
T
T
Schnelle
T
T
Müller
T
T
Leya
G
G
Fuhr
D002462:Cell Membrane / Q000737:chemistry; D000460:Chlorophyta; D003584:Cytological Techniques; D003593:Cytoplasm / Q000737:chemistry; D004574:Electromagnetic Fields; D004912:Erythrocytes; D006801:Humans; D007259:Infrared Rays; D015759:Ionomycin; D007834:Lasers; D008839:Microelectrodes; D012399:Rotation; D012441:Saccharomyces cerevisiae; D014407:Tumor Cells, Cultured
D015759:Ionomycin