The influence of low H2O2 concentrations on DNA content distribution in cell cycle and expression of cyclins in human cells.
The previous study has shown several cell lines pretreated with a low dose of hydrogen peroxide to exhibit an adaptive response to subsequent high doses of adriamycin. We undertook the present investigation to examine whether a relationship exists between the hydrogen peroxide pretreatment and perturbation in the phases of the cell cycle observed as the changes in DNA content distribution. We utilized human embryo cells, human melanoma cells and human melanoma adriamycin resistant subline, experimental developed. The data obtained in the present study suggest that hydrogen peroxide at low concentration can modify the normal rate of cell cycle progression.
Drug Institute, Warsaw, Poland.
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EL
Anuszewska
G
G
Woźniak
J
J
Skierski
D002453:Cell Cycle / Q000187:drug effects; D019926:Cyclin B / Q000096:biosynthesis; D056744:Cyclin B1; D019938:Cyclin D1 / Q000096:biosynthesis; D016213:Cyclins / Q000096:biosynthesis*; D004247:DNA / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C534699:CCNB1 protein, human; D019926:Cyclin B; D056744:Cyclin B1; D016213:Cyclins; D004273:DNA, Neoplasm; D019938:Cyclin D1; D004247:DNA; D006861:Hydrogen Peroxide