[The examination of EBV-DNase gene fragment in the paraffin embedded NPC, pre-cancerous and high risk population nasopharyngeal tissues].
OBJECTIVE
To examine if EB virus enters nasopharyngeal epithelial cells in the course of the tumorigenesis in nasopharyngeal carcinoma (NPC).


METHODS
Based on the annual investigation of Si-Hui county, a cancer-prevention base of the Sun Yet-sen University of Medical Sciences and Tumour Hospital outpatient clinic, more than 450 paraffin-embedded tissues with NPC, and with pre-cancerous lesions in high risk populations were collected for the detection of EBV-DNase gene fragment by an optimized PCR method.


RESULTS
In 145 of the 149(97.3%) cases with invasive NPC, and 2 of 4 cases with in situ NPC, EBV-DNase gene was detected. However, in Sihui, Canton, a high-risk county for NPC, only 2 of the 155 subjects with nasopharyngeal precancerous state was the gene positive, and none of the 47 cases examined in the out-patient department of the Tumor Hospital with precancerous lesions in the nasopharynx was positive.


CONCLUSIONS
EBV is seldomly detected in the non-malignant and precancerous lesions in the nasopharynx in high-risk populations. It implies that EBV is probably not etiologically involved in the genesis of NPC.
Cancer Institute, Sun Yet-sen University of Medical Sciences, Guangzhou.
B
B
Huang
D
D
Huang
Q
Q
Wu
D003851:Deoxyribonucleases / Q000235:genetics*; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000201:enzymology*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology / Q000821:virology*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000473:pathology / Q000821:virology*; D012307:Risk Factors
D003851:Deoxyribonucleases