[Studies of loss of heterozygosity(LOH) in Chinese human gastric cancer tissues].
OBJECTIVE
To identify the LOH frequency in Chinese gastric cancer samples and locate lost region of gastric cancer-related tumor suppressor gene.


METHODS
Thirty microsatellite markers were used to examine 42 cases of tumor-normal paired gastric cancer tissues for LOH on all chromosomes (except Y) using polymerase chain reaction(PCR).


RESULTS
The total frequency of LOH in 42 gastric cancer was 27.7%. High frequencies of LOH (> or = 40%) were observed at loci D3S1067, D3S1577, D6S430, D15S205, D17S945, and D5S407, D2S136, D2S147, D13S175, D14S68, D8S279 loci had LOH frequency > or = 30%. Significantly more LOH cases were observed in the poorly differentiated type and the signet-cell type than in well differentiated type of gastric cancers (P = 0.00699 and 0.0001). The incidence of LOH in signet-cell cancer was even significantly higher than that in poorly differentiated gastric carcinomas(P = 0.0019). LOH was not found in 4 cases with well differentiated gastric cancer.


CONCLUSIONS
The most common LOH occurred at D3S1577, D3S1067, D5S407, D6S430, D8S279, D15S205 and D17S945 might imply the existence of potential tumor suppressor genes of gastric cancer.
Laboratory of Cancer Genetics, Beijing Institute for Cancer Research, Beijing Medical University.
Y
Y
Wang
E
E
Zheng
Y
Y
Ke
D000230:Adenocarcinoma / Q000235:genetics; D000328:Adult; D018279:Carcinoma, Signet Ring Cell / Q000235:genetics; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics*