[Intracameral and lenticular penetration of locally applied deuterium-labeled vitamin E in rats].
OBJECTIVE
To confirm the intraocular dynamics of 1% deuterium (D)-labeled alpha-tocopherol acetate (VEA) solution.


METHODS
The concentrations of D3-VEA and D3-alpha-tocopherol (VE) derived from D3-VEA in the aqueous humor and lens were measured after instilling 1% D3-VEA continuously into the cul-de-sac of rat eyes for one and three weeks. D3-VEA and D3-VE concentrations were determined by gas chromatograph/mass spectrometry.


RESULTS
D3-VEA and D3-VE concentrations in the aqueous humor after one and three weeks of continuous administration were 93.1 and 498.9, and 9.4 and 21.5 ng/ml, respectively. The concentrations in the lens were 15.0 and 6.1, and 9.8 and 4.8 ng/g, respectively.


CONCLUSIONS
The penetration of VEA into the aqueous humor and lens by eyedrop application was confirmed.
Department of Ophthalmology, Kanazawa Medical University, Japan.
M
M
Nagata
K
K
Kawazu
Y
Y
Midori
M
M
Kojima
E
E
Shirasawa
K
K
Sasaki
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D003903:Deuterium; D005123:Eye; D005260:Female; D007322:Instillation, Drug; D007908:Lens, Crystalline / Q000378:metabolism*; D051381:Rats; D011914:Rats, Inbred BN; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D014810:Vitamin E; D003903:Deuterium; D024502:alpha-Tocopherol; D024505:Tocopherols