Neuronal propagation of HSV1 from the oral mucosa to the eye.
OBJECTIVE
To identify possible neuronal pathways leading to herpetic ocular disease after primary oral infection in mice.


METHODS
The SC16 strain of herpes simplex virus (HSV)-1 (10(6) plaque-forming units) was injected into the mucocutaneous border of the left upper lip. Animals were killed 2 to 10 days postinoculation (DPI). Spread of the virus in neural structures was studied by immunochemistry.


RESULTS
HSV1 first replicated at the site of inoculation and then at the superior cervical ganglion (at 2 DPI). The trigeminal ganglion and the facial nerve fibers were infected by 4 DPI. Infection of the ciliary body and iris occurred at 6 DPI, together with several brain stem nuclei belonging to the autonomic or sensory pathways. Between 8 and 10 DPI, the neural infection gradually cleared up, except for the ipsilateral sympathetic ganglion, and ipsilateral keratitis appeared in some animals.


CONCLUSIONS
The pattern of viral dissemination in this mouse model suggests that infection of iris and ciliary body results from transfer of virus in the superior cervical ganglion from sympathetic neurons innervating the lip to neighboring neurons innervating the anterior uvea. Later, zosteriform spread of virus from the trigeminal system may have contributed to the clinical and histologic findings.
Labetoulle
M
M
Laboratoire de Génétique des Virus, Centre National de la Recherche Scientifique, Gif-sur Yvette, France. marc.labetoulle@gv.cnrs-gif.fr
Kucera
P
P
Ugolini
G
G
Lafay
F
F
Frau
E
E
Offret
H
H
Flamand
A
A
D000818:Animals; D001933:Brain Stem / Q000821:virology; D002924:Ciliary Body / Q000294:innervation / Q000821:virology; D006224:Cricetinae; D015828:Eye Infections, Viral / Q000473:pathology / Q000821:virology*; D005154:Facial Nerve / Q000821:virology; D005260:Female; D006561:Herpes Simplex / Q000473:pathology / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification / Q000502:physiology*; D007498:Iris / Q000294:innervation / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009061:Mouth Mucosa / Q000294:innervation* / Q000473:pathology / Q000821:virology; D009434:Neural Pathways / Q000821:virology*; D017783:Superior Cervical Ganglion / Q000821:virology*; D013997:Time Factors; D012668:Trigeminal Ganglion / Q000821:virology; D014606:Uveitis, Anterior / Q000473:pathology / Q000821:virology*; D014779:Virus Replication / Q000502:physiology*