Human epithelial cell death caused by Actinobacillus actinomycetemcomitans infection.
The gingival sulcus is the shallow crevice around the tooth, and its epithelium is a gateway for initial bacterial infection in periodontal disease. Recent studies have shown that Actinobacillus actinomycetemcomitans invades an epithelial cell line, KB cells, in vitro. The aim of the present study was to clarify the changes in KB cells after A. actinomycetemcomitans infection. The cytotoxic effects of A. actinomycetemcomitans on KB cells were determined at 72, 96 and 120 h after infection by an MTT assay. Nuclear morphological changes were observed by staining with Hoechst 33258. Cytoplasmic histone-associated DNA fragmentation in the infected KB cells was determined by ELISA. A. actinomycetemcomitans was cytotoxic on KB cells, and condensation and degradation of the nuclei were observed. DNA fragmentation was increased after the infection. In addition, A. actinomycetemcomitans showed similar cytotoxic effects on human gingival epithelial cells. The present study demonstrated that A. actinomycetemcomitans induces apoptotic cell death of oral epithelial cells in an in-vitro culture system. This induced apoptosis might be involved in the initiation and progression of periodontitis.
Satsuki
S
Kato
Keisuke
K
Nakashima
Maki
M
Inoue
Jun
J
Tomioka
Koji
K
Nonaka
Department of Periodontology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido and *Department of Oral Science, National Institute of Infectious Diseases, Tokyo, Japan.
Tatsuji
T
Nishihara
Yusuke
Y
Kowashi
D016976:Aggregatibacter actinomycetemcomitans / Q000472:pathogenicity*; D017209:Apoptosis; D015638:Cytochalasin D / Q000494:pharmacology; D053938:DNA Fragmentation; D005881:Gingiva / Q000473:pathology; D006801:Humans; D007624:KB Cells; D010510:Periodontal Diseases / Q000209:etiology
D015638:Cytochalasin D