Identification of a taurine transport inhibitory substance in sesame seeds.
An ethanol extract from sesame seeds inhibited the taurine uptake in human intestinal epithelial Caco-2 cells. The uptake of such alpha-amino acids as leucine and glutamic acid was not inhibited by the extract, indicating that this inhibition is specific to the taurine uptake. The unknown inhibitor in the sesame extract was purifled by reversed-phase HPLC by monitoring the inhibitory effect on taurine uptake. The isolated substance was identified as lysophosphatidylcholine, linoleoyl (Lyso-PC), by NMR and MS analysis. Lyso-PC inhibited the taurine uptake in a dose-dependent manner with an IC50 value of approximately 200 microM. Although Lyso-PC is known to be a surface active and cell lytic compound, neither damage nor loss of integrity of the Caco2 cell monolayer was apparent after treating with 200 microM Lyso-PC. Inhibition was observed by incubating cells with Lyso-PC for only 1 min prior to the uptake experiments. These results suggest the direct effect of Lyso-PC on the cell membrane to be the main mechanism for this inhibition. Lyso-PC may play a role in the regulation of certain intestinal transporters.
Department of Applied Biological Chemistry, The University of Tokyo, Yayoi, Japan.
K
K
Ishizuka
A
A
Kanayama
H
H
Satsu
Y
Y
Miyamoto
K
K
Furihata
M
M
Shimizu
D001693:Biological Transport, Active / Q000187:drug effects; D018938:Caco-2 Cells; D005504:Food Analysis; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D013654:Taurine / Q000378:metabolism*
D008244:Lysophosphatidylcholines; D010936:Plant Extracts; D013654:Taurine