Search for UV-responsive genes in human cells by differential mRNA display: involvement of human ras-related GTP-binding protein, Rheb, in UV susceptibility.
The search for genes responsible for the sensitivity of human cells to cell-killing effects of UV is an important area of biological research. To identify candidate genes responsible for UV sensitization, levels of mRNA expression were compared between UV-sensitive RSa cells and UV-resistant variant UV(r)-1 cells, using a differential display method and Northern blot analysis. Messenger RNA expression levels of human Ras homologue enriched in brain (Rheb) and/or a Rheb-like gene were up-regulated and slightly decreased in UV-irradiated RSa and UV(r)-1 cells, compared to in mock-irradiated cells, respectively. RSa and UV(r)-1 cells, both of which were treated with antisense oligonucleotides for Rheb RNA, exhibited an increased resistance to UV cell-killing. It remains unclear why UV(r)-1 cells are resistant to UV yet express Rheb mRNA at high levels. However, the results of antisense experiments together with the up-regulation in UV-irradiated RSa cells, suggest that Rheb is involved in the UV sensitization of both cells to UV cell-killing.
Kita
K
K
Department of Biochemistry, Department of Surgery, School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba City, Chiba, 260, Japan. kita@med.m.chiba-u.ac.jp
Wu
Y P
YP
Sugaya
S
S
Moriya
T
T
Nomura
J
J
Takahashi
S
S
Yamamori
H
H
Nakajima
N
N
Suzuki
N
N
D016923:Cell Death / Q000235:genetics / Q000528:radiation effects; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D015723:Gene Library; D011905:Genes, ras; D006801:Humans; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D000076205:Ras Homolog Enriched in Brain Protein; D014466:Ultraviolet Rays
D009479:Neuropeptides; C490211:RHEB protein, human; D012333:RNA, Messenger; D000076205:Ras Homolog Enriched in Brain Protein; D020559:Monomeric GTP-Binding Proteins