Validation of a capillary electrophoresis method for determination of 5-aminolevulinic acid and degradation products.
A capillary electrophoresis method was developed for simultaneous quantification of 5-aminolevulinic acid (ALA) and its degradation products 2,5-dicarboxyethyl-3,6-dihydropyrazine and 2,5-dicarboxyethylpyrazine in aqueous solution within a total analysis time of 9 min. The optimized method was validated with respect to specificity, precision, linearity, limits of detection and quantitation, and robustness. The degradation products were quantified with respect to the ALA peak. A related micellar electrokinetic chromatography method, involving the addition of sodium dodecylsulfate to the running buffer solution, was applied for direct injection of an oil-in-water emulsion containing ALA, i.e. without sample pretreatment.
Swiss Federal Institute of Technology Zurich (ETH), Department of Applied BioSciences, Institute of Pharmaceutical Sciences.
A
A
Bunke
H
H
Schmid
G
G
Burmeister
H P
HP
Merkle
B
B
Gander
D000622:Aminolevulinic Acid / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000622:Aminolevulinic Acid