Beta-1,3-glucooligosaccharide induced activation of four enzymes responsible for N-p-coumaroyloctopamine biosynthesis in potato (Solanum tuberosum cv.) tuber tissue.
Potato tuber disks, when treated with laminarin, a beta-1,3-glucooligosaccharide from Laminaria digitata, accumulate a hydroxycinnamoyl amide compound, N-p-coumaroyloctopamine (p-CO). The biosynthesis of p-CO was investigated by feeding experiments, in order to show that the precursors of N-p-coumaroyl and octopamine moieties of p-CO are L-phenylalanine and L-tyrosine, respectively. The treatment of potato tuber tissue with laminarin resulted in elevated activities of four enzymes which are putatively involved in p-CO biosynthesis: phenylalanine ammonia lyase (PAL; EC 4.3.1.5), 4-hydroxycinnamic acid:CoA ligase (4CL; EC 6.2.1.12), hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase (THT; EC 2.3.1.110) and tyrosine decarboxylase (TyrDC; EC 4.1.1.25). Among these, the response of TyrDC was specific to laminarin treatment, thus indicating that the regulation of TyrDC activity is critical for the accumulation of p-CO in potato tuber tissue.
Matsuda
F
F
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan. matsuda@colorado.kais.kyoto-u.ac.jp
Miyagawa
H
H
Ueno
T
T
D000217:Acyltransferases / Q000378:metabolism; D003066:Coenzyme A Ligases / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism*; D004789:Enzyme Activation; D005936:Glucans; D007700:Kinetics; D009655:Octopamine / Q000031:analogs & derivatives* / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology; D011134:Polysaccharides / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000201:enzymology*; D014445:Tyrosine Decarboxylase / Q000378:metabolism
D003373:Coumaric Acids; D005936:Glucans; C410948:N-4-coumaroyloctopamine; D011134:Polysaccharides; D009655:Octopamine; C008247:laminaran; D000217:Acyltransferases; C108168:hydroxycinnamoyl-CoA-tyramine N-(hydroxycinnamoyl)transferase; D014445:Tyrosine Decarboxylase; D010650:Phenylalanine Ammonia-Lyase; D003066:Coenzyme A Ligases; C019943:4-coumarate-CoA ligase