[Expression and significance of interleukin 6, interleukin 8 and their receptors in acute promyelocytic leukemia during all-trans retinoic acid induction treatment].
OBJECTIVE
To evaluate the expression and clinical significance of interleukin 6, soluble glycoprotein 130 (sgp 130), interleukin 8 and type A interleukin 8 receptor (IL-8RA) in acute promyelocytic leukemia (APL) patients during all-trans retinoic acid (ATRA) treatment.


METHODS
Serum and bone marrow mononuclear cell (MNC) culture supernatant IL-6, sgp 130, IL-8 concentrations of 18 cases APL patients were measured (ELISA). Bone marrow MNC IL-8RA was measured by flow cytometry after cultured with ATRA (10(-6) mmol/L).


RESULTS
Serum IL-6, sgp130, IL-8 levels were higher than normal (P < 0.05), IL-6, sgp130 levels correlated with white blood cell (WBC) counts (P < 0.05) while IL-8 level correlated with body temperature (P < 0.05) at initial diagnosis after 72-hour incubation with ATRA, concentration of IL-6 of bone marrow MNC culture supenatant did not change, that of sgp130 mildly decreased, and IL-8 significantly decreased while the positive rate of IL-8RA on bone marrow MNC increased. During ATRA treatment, serum IL-6 changes were correlated with WBC changes. Peak level of IL-6 and WBC was lower in patients received intermittent therapy than continuous therapy. Serum IL-6 and IL-8 increased when complicated with infection and increase in IL-8 seemed more sensitive.


CONCLUSIONS
Serum levels of IL-6, sgp130, IL-8 may reflect patient's responsiveness to ATRA treatment, predict hyperleukocytosis and intercurrent infection. ATRA induces APL cell differentiation possibly via gp130 signal transduction.
First Affiliated Hospital, Suzhou Medical College, Jiangsu Institute of Hematology, Suzhou.
H
H
Zeng
X
X
Zhang
Z
Z
Chen
D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000097:blood; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged; D018123:Receptors, Interleukin / Q000097:blood; D023062:Receptors, Interleukin-8A; D015398:Signal Transduction; D014212:Tretinoin / Q000627:therapeutic use*
D015703:Antigens, CD; D006023:Glycoproteins; D015850:Interleukin-6; D016209:Interleukin-8; D018123:Receptors, Interleukin; D023062:Receptors, Interleukin-8A; D014212:Tretinoin