[Liver metastasis models of human colo-rectal carcinoma established in nude mice by orthotopic transplantation and their biologic characteristics].
OBJECTIVE
To establish a liver metastasis models of human colorectal carcinomas in nude mice.


METHODS
Orthotopic transplantation of histologically intact colo-rectal cancer tissues from patients into colo-rectal mucosa of nude mice. Tumorgenicity, invasion, metastasis and morphological characteristics of the transplanted tumors were studied by light microscopy, electron microscopy and immunuhistochemistry.


RESULTS
Liver metastasis models of human colon carcinoma (HCA-HMN-1) and human rectal carcinoma (HRA-HMN-2) were established after screening from 34 colorectal carcinomas. They had been passaged in vivo for 18 and 21 gererations respectively. There were lymphatic, hematogenous, and implanting metastases. CEA secretion was maintained after transplantation. The primary and liver metastatic tumors were similar to the original human carcinoma in histopathological and ultrastructural features, DNA content and chromosomal karyotype.


CONCLUSIONS
The liver metastasis models provide useful tools for the study of mechanism of metastasis and its treatment of human colo-rectal cancer.
Department of Hepato-biliary Surgery, 202 Hospital of PLA, Shenyang.
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Q
Liu
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Tuo
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B
Wu
D000230:Adenocarcinoma / Q000473:pathology / Q000556:secondary*; D000328:Adult; D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D012004:Rectal Neoplasms / Q000473:pathology*