Lack of nitric oxide- and guanosine 3':5'-cyclic monophosphate-dependent regulation of alpha-thrombin-induced calcium transient in endothelial cells of spontaneously hypertensive rat hearts.
While the expression and/or activity of endothelial nitric oxide synthase (eNOS) has been characterized in spontaneously hypertensive (SHR) and normotensive Wistar Kyoto rat (WKY) hearts, in coronary endothelial cells (ECs) from both strains, the effect of NO on intracellular calcium concentration ([Ca(2+)](i)) is still unknown. Coronary microvascular ECs were isolated from SHR and WKY and characterized. Immunocytochemistry and Western blot analysis showed that eNOS was similarly expressed in ECs from both strains. Measuring [Ca(2+)](i) by imaging analysis of fura-2-loaded cells, we demonstrated that alpha-thrombin (3-180 U l(-1)) induced a superimposable dose-dependent calcium transient in ECs from both strains. In WKY ECs, S-nitroso-N-acetyl-DL-penicillamine (SNAP) dose-dependently (10 - 100 microM) and 0.1 microM atrial natriuretic factor (ANF) reduced the maximum and the decay time of alpha-thrombin-induced calcium transient. The inhibitory effects of SNAP and ANF were prevented by blocking cyclic GMP-dependent protein kinase. Non selective eNOS inhibitors prolonged the decay time of alpha-thrombin-induced calcium transient, while the selective inducible NOS inhibitor 1400 W was ineffective. SNAP (100 microM) and 0.1 microM ANF increased cyclic GMP content up to 22.9 and 42.3 fold respectively. In SHR ECs, alpha-thrombin-induced calcium transient was not modified by SNAP, ANF or eNOS inhibition. SNAP (100 microM) and 0.1 microM ANF increased cyclic GMP content up to 9. 3 and 51 fold respectively. In WKY ECs, SNAP dose-dependently (10 - 100 microM) reduced also bradykinin-induced calcium transient, while in SHR ECs was ineffective. We concluded that in SHR ECs, the cyclic GMP-dependent regulation of calcium transient is lost.
Failli
P
P
Department of Pharmacology, Viale Pieraccini, 6 50139 Florence, Italy. Failli@server1.pharm.unifi.it
Fazzini
A
A
Ruocco
C
C
Mazzetti
L
L
Cecchi
E
E
Giovannelli
L
L
Marra
F
F
Milani
S
S
Giotti
A
A
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001920:Bradykinin / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D029721:Drosophila Proteins; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism*; D019476:Insect Proteins / Q000378:metabolism; D009206:Myocardium / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016601:RNA-Binding Proteins; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013917:Thrombin / Q000378:metabolism
D029721:Drosophila Proteins; D019476:Insect Proteins; D020030:Nitric Oxide Donors; D016601:RNA-Binding Proteins; C110881:S-nitro-N-acetylpenicillamine; C070574:nos protein, Drosophila; D009569:Nitric Oxide; D009320:Atrial Natriuretic Factor; D013917:Thrombin; D010396:Penicillamine; D006152:Cyclic GMP; D001920:Bradykinin; D002118:Calcium