Nerve growth factor-potentiating compounds from Picrorhizae Rhizoma.
A crude methanol extract of Picrorhizae Rhizoma, the dried underground parts of Picrorhiza scrophulariiflora PENNELL, has been shown to potentiate the nerve growth factor (NGF)-induced neurite outgrowth from PC12D cells. The methanol extract was partitioned between ethyl acetate and water. The NGF-potentiating activity was observed in the ethyl acetate fraction. The ethyl acetate solubles were fractionated by silica gel chromatography, monitoring the NGF-potentiating activity to give two iridoid glycosides, picrosides I and II. Picrosides did not exhibit neurotrophic activity but caused a marked enhancement of the NGF-mediated neurite outgrowth from PC12D cells. The pharmacological data suggest that picrosides I and II enhance neurite outgrowth from PC12D cells, probably by amplifying a step in the NGF-receptor-mediated intracellular signaling pathway.
Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Sendai, Japan.
P
P
Li
K
K
Matsunaga
Y
Y
Ohizumi
D000818:Animals; D048429:Cell Size / Q000187:drug effects; D002934:Cinnamates / Q000302:isolation & purification / Q000494:pharmacology*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D057888:Iridoid Glucosides; D019684:Magnoliopsida; D020932:Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats
D002934:Cinnamates; D005960:Glucosides; D057888:Iridoid Glucosides; D010936:Plant Extracts; C109270:picroside I; C416837:picroside II; D020932:Nerve Growth Factor