High levels of IL-10 and determination of other cytokines and chemokines in HIV-associated haemophagocytic syndrome.
Haemophagocytic syndrome (HPS) and HIV infection are both associated with cytokine network dysregulation. We therefore analysed plasma levels and mRNA synthesis in peripheral blood mononuclear cells (PBMC) of cytokines, chemokines and chemokine receptors in one HIV-infected patient with HPS. We compared the results with those for eight HIV-infected patients with similar CD4+ T cell counts (207/mm3 versus controls: median 214/mm3) and plasma virus load (4.1 log copies/ml, versus controls: median 4.2 log copies/ml). The HPS patient had a lower viral DNA load in PBMC and higher plasma levels of interferon-gamma, IL-10, and macrophage inflammatory protein (MIP)-1beta. No difference in plasma tumour necrosis factor-alpha (TNF-alpha), IL-6 and MIP-1alpha concentration was observed between the HPS patient and control patients. No difference was observed in TNF-alpha, IL-1beta, IL-10, IL-4, MIP-1alpha, MIP-1beta, RANTES, CXCR-4, and CCR-5 mRNA levels in PBMC, but IL-6 levels were higher in the HPS patient. Our results emphasize the role of IL-10 in the control of immune hyperactivation that is observed in HPS.
Benveniste
O
O
CEA, Service de Neurovirologie, CRSSA, DRM/DSV, Institut Paris-Sud sur les Cytokines, B.P. 6, Fontenay aux Roses Cedex, France. olivier.benveniste@psl.ap-hop-paris.fr
Dereuddre-Bosquet
N
N
Clayette
P
P
Leport
C
C
Vildé
J L
JL
Dormont
D
D
D000328:Adult; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000032:analysis / Q000235:genetics; D018925:Chemokines / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000150:complications*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D015616:Histiocytosis, Non-Langerhans-Cell / Q000097:blood* / Q000209:etiology; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics; D016753:Interleukin-10 / Q000097:blood*; D007378:Interleukins / Q000032:analysis / Q000235:genetics; D019402:Macrophage Inflammatory Proteins / Q000032:analysis / Q000235:genetics; D011533:Proviruses / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000097:blood; D019713:Receptors, CCR5 / Q000032:analysis / Q000235:genetics; D019718:Receptors, CXCR4 / Q000032:analysis / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D018925:Chemokines; D016207:Cytokines; D004279:DNA, Viral; D007378:Interleukins; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma