Mutational analysis of HIV-1 gag proteins (review).
The mature Gag proteins of human immunodeficiency virus type 1 (HIV-1) are major components of infectious virions, and thought to carry out numerous functions throughout the HIV-1 replication cycle. We have recently generated numerous gag gene mutants of HIV-1 to genetically study the functions of the Gag proteins. Through the biological and biochemical analyses, our HIV-1 gag mutants have been grouped into early (defective for uncoating/reverse transcription), late (defective for virion release/maturation), and early/late (defective for both steps) mutants. Many mutants are found to efficiently inhibit the replication of wild-type virus. Worthy of note, there are some early mutants which show host cell-dependent replication potential.
Department of Virology, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan.
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Miyaura
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Yoshida
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Sakurai
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Fujita
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Koyama
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Adachi
D004252:DNA Mutational Analysis; D003673:Defective Viruses / Q000235:genetics; D015683:Gene Products, gag / Q000096:biosynthesis / Q000235:genetics* / Q000627:therapeutic use; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D014779:Virus Replication / Q000235:genetics
D015683:Gene Products, gag