[Construction of PCNA antisense RNA expression vector and its antitumorigenic effect on human gastric cancer cell].
OBJECTIVE
To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.


METHODS
An eukaryotic expression vector pDR-PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC-7901 was transfected with pDR-PCNA by lipofectamine.


RESULTS
Growth rate, protein and RNA biosynthesis and DNA contents in S-phase and G2/M-phase of the transfected SGC-7901 were significantly suppressed in comparison with those of the parental cell line.


CONCLUSIONS
PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.
Department of Gastroenterology, 210 Hospital of PLA, Dalian.
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Dai
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Zhang
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Hui
D002455:Cell Division; D004273:DNA, Neoplasm / Q000378:metabolism; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D016372:RNA, Antisense / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen; D016372:RNA, Antisense