Antibacterial and allelopathic activity of extract from Castanea sativa leaves.
Following the extraction of Castanea sativa with an aqueous solution of sulfuric acid (pH 3.0), the ethyl acetate soluble fraction was tested for its antibacterial and allelopathic activity. The extract was shown to have pronounced antibacterial effects against seven of the eight strains of Gram-positive and Gram-negative bacteria used (MIC in the range of 64-256 microg/ml and MBC in the range of 256-512 microg/ml). The active fraction was analyzed by TLC and HPLC showing the presence of rutin, hesperidin, quercetin, apigenin, morin, naringin, galangin and kaempferol. Standards of the identified flavonoids were tested against the same bacterial strains. The highest activity was shown by quercetin, rutin and apigenin. The allelopathic effect was tested against Raphanus sativus seed germination. The extract, quercetin, rutin and apigenin caused a decrease in the percentage of seed germination and root and epicotyl growth.
Dipartimento di Biologia Vegetale, Università degli Studi di Napoli 'Federico II', via Foria 223, 80139 Naples, Italy. adbasile@unina.it
A
A
Basile
S
S
Sorbo
S
S
Giordano
L
L
Ricciardi
S
S
Ferrara
D
D
Montesano
R
R
Castaldo Cobianchi
M L
ML
Vuotto
L
L
Ferrara
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D029962:Fagaceae; D005419:Flavonoids / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D018517:Plant Roots; D031224:Raphanus
D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010936:Plant Extracts