Protective effect of extracts of Mesona procumbens Hemsl. on DNA damage in human lymphocytes exposed to hydrogen peroxide and UV irradiation.
In the present study, the protective effect of water extracts from Hsian-tsao (WEHT) on DNA damage in human lymphocytes induced by UV-C and/or H(2)O(2) was evaluated using single-cell electrophoresis (comet assay). No toxicity was found in WEHT towards human lymphocytes. WEHT did not cause DNA damage at lower concentrations of 0.05 and 0.1 mg/ml, while it did cause slight DNA damage at a concentration of 0.5-2.5 mg/ml when compared with the control group. When WEHT was mixed with H(2)O(2) for reaction, it exhibited a slight inhibitory effect on DNA damage induced by H(2)O(2). Moreover, when WEHT and lymphocytes were irradiated by UV-C and then incubated for 35 min, the DNA damage decreased with an increase of the concentration of WEHT. Thus, WEHT could reduce UV-C-induced DNA damage, and WEHT had a more protective effect on UV-C than on H(2)O(2)-induced DNA damage. The protective effect of WEHT on DNA damage might be due to the fact that it contains polyphenol compounds and/or other active components.
Yen
G C
GC
Department of Food Science, National Chung Hsing University, Taichung 402, ROC, Taiwan. gcyen@mail.nchu.edu.tw
Hung
Y L
YL
Hsieh
C L
CL
D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D014466:Ultraviolet Rays
D010936:Plant Extracts; D006861:Hydrogen Peroxide