Anaerovorax odorimutans gen. nov., sp. nov., a putrescine-fermenting, strictly anaerobic bacterium.
The strictly anaerobic, gram-positive, non-spore-forming bacterium strain NorPut1T ferments putrescine to acetate, butyrate, molecular hydrogen and ammonia. It also utilizes 4-aminobutyrate and 4-hydroxybutyrate as growth substrates. Comparative 16S rDNA sequence analysis confirmed a phylogenetic affiliation of this strain to the phylum of gram-positive bacteria with low DNA G+C content. Together with its closest relative, 'Clostridium aminobutyricum' (DSM 2634), and several Eubacterium species, strain NorPut1T represents a well-defined monophyletic group. Moderate overall 16S rRNA sequence similarities (< 91%) were found for the NorPut1T/'Clostridium aminobutyricum' pair and several Eubacterium species. The type species, Eubacterium limosum, is not a member of the group and, together with Eubacterium barkeri and Pseudoramibacter alactolyticus, represents a distant phylogentic cluster. Therefore, a new genus, Anaerovorax, is proposed as harbouring strain NorPut1T (= DSM 5092T), which is described as a new species, i.e. Anaerovorax odorimutans.
C
C
Matthies
S
S
Evers
W
W
Ludwig
B
B
Schink
D000085:Acetates / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000693:Anaerobiosis; D001482:Base Composition; D002087:Butyrates / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis; D005285:Fermentation; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011700:Putrescine / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D000085:Acetates; D002087:Butyrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000641:Ammonia; D006859:Hydrogen; D011700:Putrescine