Crystallization and preliminary X-ray analysis of the EVH1 domain of Vesl-2b.
Proteins of the Homer/Vesl family are enriched at excitatory synapses and selectively bind to a proline-rich consensus sequence in group 1 metabotropic glutamate receptors via a domain that shows a strong similarity to the Ena/VASP homology 1 (EVH1) domains. EVH1 domains play an important role in actin cytoskeleton dynamics. Crystals of the EVH1 domain of murine Vesl-2b were obtained that diffract X-rays to 2.4 A resolution. They belong to space group C2, with unit-cell parameters a = 112.8, b = 69.9, c = 54.9 A, beta = 110.7 degrees, consistent with three molecules per asymmetric unit and a solvent content of 53%.
Department of Structural Biology, German National Center of Biotechnology (GBF), Mascheroder Weg 1, D-38124 Braunschweig, Germany.
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M
Barzik
W D
WD
Schubert
U
U
Carl
J
J
Wehland
D W
DW
Heinz
D002352:Carrier Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D000072233:Homer Scaffolding Proteins; D009479:Neuropeptides / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry
D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; D009479:Neuropeptides; D011994:Recombinant Proteins