High-LET radiation-induced aberrations in prematurely condensed G2 chromosomes of human fibroblasts.
OBJECTIVE
To determine the number of initial chromatid breaks induced by low- or high-LET irradiations, and to compare the kinetics of chromatid break rejoining for radiations of different quality.


METHODS
Exponentially growing human fibroblast cells AG1522 were irradiated with gamma-rays, energetic carbon (290MeV/u), silicon (490MeV/u) and iron (200 and 600 MeV/u). Chromosomes were prematurely condensed using calyculin A. Chromatid breaks and exchanges in G2 cells were scored. PCC were collected after several post-irradiation incubation times, ranging from 5 to 600 min.


RESULTS
The kinetics of chromatid break rejoining following low- or high-LET irradiation consisted of two exponential components representing a rapid and a slow time constant. Chromatid breaks decreased rapidly during the first 10min after exposure, then continued to decrease at a slower rate. The rejoining kinetics were similar for exposure to each type of radiation. Chromatid exchanges were also formed quickly. Compared to low-LET radiation, isochromatid breaks were produced more frequently and the proportion of unrejoined breaks was higher for high-LET radiation.


CONCLUSIONS
Compared with gamma-rays, isochromatid breaks were observed more frequently in high-LET irradiated samples, suggesting that an increase in isochromatid breaks is a signature of high-LET radiation exposure.
Kawata
T
T
NASA Lyndon B. Johnson Space Center, Radiation Biophysics Laboratory, Houston, TX 77058, USA. tkawata@ems.jsc.nasa.gov
Gotoh
E
E
Durante
M
M
Wu
H
H
George
K
K
Furusawa
Y
Y
Cucinotta
F A
FA
D002460:Cell Line; D002842:Chromatids / Q000528:radiation effects; D002869:Chromosome Aberrations; D005347:Fibroblasts / Q000528:radiation effects; D016195:G2 Phase / Q000528:radiation effects*; D006801:Humans; D018499:Linear Energy Transfer; D012062:Relative Biological Effectiveness