T-DNA transfer to maize plants.
Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on shoots of young maize seedlings after cocultivation with Agrobacterium.
IBMP/CNRS, Strasbourg, France. Wen-Hui.Shen@ibmp-ulp.u-strasbg.fr
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D004269:DNA, Bacterial / Q000235:genetics*; D030821:Plants, Genetically Modified; D014162:Transfection / Q000379:methods*; D003313:Zea mays
D004269:DNA, Bacterial; C036483:T-DNA