Determination of cefaclor by selective sample enrichment/clean-up on silica gel bonded polyelectrolyte in ion-exchange column chromatography.
A silica gel-bound cationic polyelectrolyte, poly[N-chloranil N,N,N',N'- tetramethylethylene diammonium dichloride], modified as ion-exchanger capable of molecular recognition of beta-lactam antibiotic, was used in solid phase extraction through column chromatography for a sample clean-up and enrichment of analyte from a dilute solution. The optimum and selective sorption conditions for a model antibiotic, cefaclor, were established. The high selectivity of polymer at pH 9.5 and flow rate as high as 5 ml/min were observed for the quantitative sorption of cefaclor. The desorption by 0.1 N HCl at flow rate of 0.1 ml/min and subsequent heating at 80 degrees C for 2 h allowed the antibiotic to be detected as corresponding oxazolone form in UV-spectrophotometric and differential pulse adsorptive stripping voltammetric measurements. The potential of the suggested approach was illustrated by estimating cefaclor in urine and blood plasma samples.
Dqeartment of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi, India.
S
S
Gupta
B B
BB
Prasad
D002433:Cefaclor / Q000032:analysis* / Q000097:blood / Q000652:urine; D002511:Cephalosporins / Q000032:analysis* / Q000097:blood / Q000652:urine; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004573:Electrolytes; D012680:Sensitivity and Specificity; D058428:Silica Gel; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D002511:Cephalosporins; D004573:Electrolytes; D058428:Silica Gel; D002433:Cefaclor; D012822:Silicon Dioxide