The mouse mammary tumor virus transcription enhancers for hematopoietic progenitor and mammary gland cells share functional elements.
Expression of mouse mammary tumor virus (MMTV)-encoded superantigens in B lymphocytes is required for viral transmission and pathogenesis. We have previously established a critical role of an enhancer element within the long terminal repeat (LTR) for MMTV sag gene expression in B-lymphoid progenitor cells. We now demonstrate enhancer activity of this element in a promyelocytic progenitor cell line. We also map the position of the enhancer within the U3 region of the MMTV LTR and show that the progenitor cell enhancer shares functional elements with a previously described mammary gland-specific enhancer.
Forschungsschwerpunkt Angewandte Tumorvirologie F0400, 69120 Heidelberg, Germany. f.reuss@dkfz-heidelberg.de
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D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D005260:Female; D017930:Genes, Reporter; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000821:virology*; D008156:Luciferases / Q000235:genetics; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D020079:Terminal Repeat Sequences; D014158:Transcription, Genetic
D008156:Luciferases