Distinct VH repertoires in primary and secondary B cell lymphocyte subsets in the preimmune repertoire of A/J mice: the CRI-A idiotype is preferentially associated with the HSA(low) B cell subset.
The anti-arsonate immune response of A/J mice is characterized by the occurrence of several recurrent idiotypes with a different temporal pattern of expression. The CRI-A idiotype is typically a memory idiotype since it appears late in the primary and dominates the secondary as well as subsequent immune responses. The CRI-C idiotype is present throughout the responses, including the primary one. Naive adult A/J mice treated repeatedly with anti-mu or anti-delta monoclonal antibodies exhibit a completely different balance of HSA(low) and HSA(high) B cell subsets and an opposite idiotype profile after immunization with p-azophenylarsonate coupled to hemocyanin. Anti-mu treatment leads to a striking enhancement of the HSA(low) cell subset associated with an earlier important synthesis of CRI-A(+) antibodies, while anti-delta treatment enhances significantly the HSA(high) compartment with a strong decrease of CRI-A and persistence of CRI-C1 antibodies. Semiquantitative PCR analysis reveals that the presence of CRI-A transcripts is associated with the HSA(low) compartment, while CRI-C transcripts are mainly associated with HSA(high) B cell subsets. This has been demonstrated with spleen cells of adult A/J mice treated with anti-mu or anti-delta antibodies and also with purified B cell subsets of unimmunized adult A/J mice and on neonatal spleen cells. It appears that the memory (CRI-A) idiotype is selected into the HSA(low) B cell subset before antigen arrival.
Masungi Luko
C
C
Laboratoire de Physiologie Animale. Université Libre de Bruxelles, Gosselies, Belgium.
Vansanten
G
G
Ryelandt
M
M
Denis
O
O
Wuilmart
C
C
Andris
F
F
Van Acker
A
A
Brait
M
M
Cloquet
J P
JP
Ismaili
N
N
Nisol
F
F
Latinne
D
D
Brown
A
A
Leo
O
O
Bazin
H
H
Urbain
J
J
D000818:Animals; D000941:Antigens / Q000032:analysis*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001483:Base Sequence; D015321:Gene Rearrangement; D006433:Hemocyanins / Q000276:immunology; D007072:Immunoglobulin D / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000235:genetics; D007075:Immunoglobulin M / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007156:Immunologic Memory; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
D000941:Antigens; D007072:Immunoglobulin D; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin