Impact of uremia on female reproductive cyclicity, ovulation, and luteinizing hormone in the rat.
BACKGROUND
Impaired reproductive function accompanies chronic renal insufficiency (uremia) in both the human and experimental animal. Clinical hypogonadism occurs in both genders. The present studies were designed to investigate possible anti-ovulatory effects of uremia in the female rat, a species that produces multiple ova during the normal estrous cycle.


METHODS
Renal insufficiency (uremia) was induced by 5/6 nephrectomy. Two control groups comprised sham-operated animals fed ad libitum (sham) or pair-wise with the uremic animals (pair-fed). Estrous cycles were determined by cytology of vaginal lavage. We examined concomitant changes in the preovulatory luteinizing hormone (LH) surge by radioimmunoassay (RIA), immunoradiometric assay (IRMA), and bioassay. Repetitive LH measurements were made from blood samples taken by intra-atrial catheter throughout the afternoon and evening of proestrus. The following morning (estrus), ovaries were collected, and ova were enumerated per oviduct.


RESULTS
Experimentally uremic animals manifested a threefold elevation of plasma creatinine and urea nitrogen and concomitantly a more than 50% impoverishment of ova production. Analyses of a large group of animals (N = 83) by RIA revealed uremia-associated attenuation of the preovulatory LH surge. Further measurements of the preovulatory LH surge by independent IRMA and LH bioassay (N = 26) corroborated this attenuation. Additional experiments indicated that these hormonal changes, but not changes in ovulation, might further reflect modulation of LH release by the anesthesia used in the preparative nephrectomy and catheterization surgeries. When normalized to body weight, the ovaries of uremic rats were found to weigh more than those of either the sham or pair-fed animals.


CONCLUSIONS
The present experiments take advantage of an experimental uremic model to document a consistent decrease in the number of ova released during estrus in the uremic animal. Possible disruption of hypothalamic-pituitary-ovarian regulation is further highlighted by attenuation in the preovulatory LH surge. These results provide a basis for further studies of neuroendocrine pathophysiology in a rodent model of uremia-associated ovulatory disruption.
Departments of Pediatrics, Biochemistry and Molecular Biophysics, and Anatomy, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond 23298, USA. krieg@hsc.vcu.edu
R J
RJ
Krieg
K
K
Tokieda
J C
JC
Chan
J D
JD
Veldhuis
D000568:Amenorrhea / Q000503:physiopathology; D000758:Anesthesia; D000818:Animals; D001806:Blood Urea Nitrogen; D002478:Cells, Cultured; D003404:Creatinine / Q000097:blood; D004971:Estrus / Q000502:physiology*; D005260:Female; D006801:Humans; D007668:Kidney / Q000166:cytology; D007986:Luteinizing Hormone / Q000097:blood*; D009392:Nephrectomy; D010060:Ovulation / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051437:Renal Insufficiency / Q000503:physiopathology; D014511:Uremia / Q000503:physiopathology*
D007986:Luteinizing Hormone; D003404:Creatinine