A variation of the amplified-fragment length polymorphism (AFLP) technique using three restriction endonucleases, and assessment of the enzyme combination BglII-MfeI for AFLP analysis of Salmonella enterica subsp. enterica isolates.
We have performed amplified-fragment length polymorphism (AFLP) fingerprinting on a collection of Salmonella enterica subsp. enterica serovar typhimurium strains with a restriction endonuclease combination (BglII and MfeI) that has previously been used successfully for typing Campylobacter jejuni isolates with high resolution. Additionally, a variation of the AFLP assay in which two rare cutting restriction enzymes (XbaI and BsrGI) in combination with the frequent cutter (HinP1I) was examined. The BglII and MfeI enzyme combination offered low resolution for genotyping Salmonella typhimurium isolates and is not recommended for this common serovar. The three-enzyme combination gave a higher discrimination, and is thus a new alternate way of performing AFLP fingerprinting of S. typhimurium.
Department of Bacteriology, National Institute of Public Health, Oslo, Norway. bjorn-arne.lindstedt@folkehelsa.no
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Lindstedt
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Heir
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Vardund
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Kapperud
D001426:Bacterial Proteins; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005838:Genotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics*; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; C059776:endodeoxyribonuclease MfeI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific