Methylopila helvetica sp. nov. and Methylobacterium dichloromethanicum sp. nov.--novel aerobic facultatively methylotrophic bacteria utilizing dichloromethane.
Eight strains of Gram-negative, aerobic, asporogenous, neutrophilic, mesophilic, facultatively methylotrophic bacteria are taxonomically described. These icl- serine pathway methylobacteria utilize dichloromethane, methanol and methylamine as well as a variety of polycarbon compounds as the carbon and energy source. The major cellular fatty acids of the non-pigmented strains DM1, DM3, and DM5 to DM9 are C18:1, C16:0, C18:0, Ccy19:0 and that of the pink-pigmented strain DM4 is C18:1. The main quinone of all the strains is Q-10. The non-pigmented strains have similar phenotypic properties and a high level of DNA-DNA relatedness (81-98%) as determined by hybridization. All strains belong to the alpha-subgroup of the alpha-Proteobacteria. 16S rDNA sequence analysis led to the classification of these dichloromethane-utilizers in the genus Methylopila as a new species - Methylopila helvetica sp.nov. with the type strain DM9 (=VKM B-2189). The pink-pigmented strain DM4 belongs to the genus Methylobacterium but differs from the known members of this genus by some phenotypic properties, DNA-DNA relatedness (14-57%) and 16S rDNA sequence. Strain DM4 is named Methylobacterium dichloromethanicum sp. nov. (VKM B-2191 = DSMZ 6343).
Doronina
N V
NV
Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino.
Trotsenko
Y A
YA
Tourova
T P
TP
Kuznetsov
B B
BB
Leisinger
T
T
D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D004275:DNA, Ribosomal / Q000235:genetics; D006088:Gram-Negative Aerobic Bacteria / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D008752:Methylene Chloride / Q000378:metabolism*; D020580:Methylobacterium / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D008752:Methylene Chloride