The ethanol-soluble part of a hot-water extract from Artemisia iwayomogi inhibits liver fibrosis induced by carbon tetrachloride in rats.
This study was carried out to investigate the protective effects of the hot-water extract from Artemisia iwayomogi (Compositae) on carbon tetrachloride-induced liver fibrosis in rats. Liver injury was induced by oral administration of carbon tetrachloride (1 mL kg(-1)) twice a week during 4 weeks of A. iwayomogi treatment. Extracts from A. iwayomogi were prepared and administered to rats orally (2 g kg(-1) as A. iwayomogi for 4 weeks) as follows: group 1, hot-water extract; group 2, ethanol-soluble part of hot-water extract; group 3, ethanol-insoluble part of hot-water extract; and group 4, methanol extract. In rats treated with the ethanol-soluble part of the hot-water extract, liver hydroxyproline content was reduced to 74% that of carbon tetrachloride control rats (P < 0.05). Protein expression of alpha smooth muscle cell like actin was also decreased in rats treated with the ethanol-soluble part of the hot-water extract, which indicates inhibition of hepatic stellate cell activation. Liver malondialdehyde levels were significantly lowered in rats treated with the ethanol-soluble part of hot-water extract (P < 0.05). Serum cholesterol levels in rats treated with hot-water extract, ethanol-soluble or -insoluble parts of hot-water extract or methanol extract were significantly reduced when compared with those of carbon tetrachloride control rats (P < 0.05). The ethanol-soluble part of the hot-water extract from A. iwayomogi inhibited fibrosis and lipid peroxidation in rats with liver fibrosis induced by carbon tetrachloride. Both hot-water extract (either ethanol-soluble or -insoluble) and methanol extract of A. iwayomogi also lowered serum cholesterol levels in fibrotic rats.
Park
E J
EJ
College of Pharmacy, Medical Resources Research Center, Wonkwang University, Iksan, Cheonbuk, South Korea.
Nan
J X
JX
Kim
J Y
JY
Kang
H C
HC
Choi
J H
JH
Lee
S J
SJ
Lee
B H
BH
Kim
S J
SJ
Lee
J H
JH
Kim
Y C
YC
Sohn
D H
DH
D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D002251:Carbon Tetrachloride; D004195:Disease Models, Animal; D000431:Ethanol / Q000737:chemistry; D006358:Hot Temperature; D006909:Hydroxyproline / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000627:therapeutic use; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000737:chemistry
D010936:Plant Extracts; D014867:Water; D000431:Ethanol; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D006909:Hydroxyproline