[Evolving methods in immunohematology].
The immunogenic nature of erythrocyte polymorphism is in variance with the incompatible transfusion. Indeed, the fixing of an antibody on the corresponding antigen generally condemns the cell concerned with its destruction. Therefore, in order to ensure the immunohemolytic safety of the transfusions, it is necessary to avoid an in vivo encounter between antigens and antibodies, whose feasibility study in vitro is a determining element. Because of the requirement standards of such analyses and the preoccupation with the continuous improvement of transfusion safety, the evolution of the methods used in immunohematology is a constant concern for all those involved in the process. Thus, during the last few years, new technologies have been introduced which aim at improving performance and sometimes implementing alternatives to agglutination. This improvement is not limited to the search for an overall increase in specificity-sensitivity; it also takes into account the capability to detect "the clinically significant" as well as the limitations of human reliability, which justifies the introduction of automation and computerization. The whole of these methodological evolutions associated with that of the performance of reagents, legitimate the need to reconsider the realization of erythrocyte typing and the search for anti-erythrocyte antibodies.
EFS Alpes-Méditerranée, Marseille, France.
J
J
Chiaroni
L
L
Mannessier
D000372:Agglutination Tests / Q000295:instrumentation; D000937:Antigen-Antibody Reactions; D001331:Automation; D001789:Blood Group Antigens / Q000032:analysis* / Q000276:immunology; D001788:Blood Grouping and Crossmatching / Q000379:methods / Q000592:standards / Q000639:trends*; D004910:Erythrocyte Membrane / Q000276:immunology*; D005374:Filtration; D006801:Humans; D007163:Immunosorbent Techniques; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000032:analysis; D015588:Observer Variation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
D001789:Blood Group Antigens; D007202:Indicators and Reagents; D007518:Isoantibodies