Propagation of spontaneous synchronized activity in cortical slice cultures recorded by planar electrode arrays.
The spatial propagation of synchronized activity in cortical slice cultures was characterized by multi-site extracellular recording. Spontaneous activity was studied in normal culture medium, and in bicuculline- or kainic acid-containing media. A common feature in all these conditions was that activity was generated first in superficial layers (i.e., layer I/II) before spreading over the whole area of the slice. In culture medium or bicuculline-containing medium, the initiation site of the activity was not constant and showed a large variety of patterns of horizontal propagation. Kainic acid induced epileptiform activity, consisting of intense initial bursts followed by repetitive after-discharges. Though the patterns of spatial propagation of the bursts were variable as in the other conditions, the after-discharges followed a constant path. Cross-correlation analysis indicated that the network moved in a graded fashion to a steady state during the sequence of after-discharges.
Jimbo
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Basic Research Laboratories, Kanagawa, Japan. jimbo@will.brl.ntt.co.jp
Robinson
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HP
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology*; D003470:Culture Media; D046508:Culture Techniques; D004566:Electrodes; D007608:Kainic Acid / Q000494:pharmacology; D051381:Rats; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology
D003470:Culture Media; D007608:Kainic Acid; D001640:Bicuculline