A new lectin in red kidney beans called PvFRIL stimulates proliferation of NIH 3T3 cells expressing the Flt3 receptor.
A new legume lectin has been identified by its ability to specifically stimulate proliferation of NIH 3T3 fibroblasts expressing the Flt3 tyrosine kinase receptor. The lectin was isolated from conditioned medium harvested from human peripheral blood mononuclear cells activated to secrete cytokines by a crude red kidney bean extract containing phytohemagglutinin (PHA). Untransfected 3T3 cells and 3T3 cells transfected with the related Fms tyrosine kinase receptor do not respond to this lectin, which we called PvFRIL (Phaseolus vulgaris Flt3 receptor-interacting lectin). When tested on cord blood mononuclear cells enriched for Flt3-expressing progenitors, purified PvFRIL fractions maintained a small population of cells that continued to express CD34 after 2 weeks in suspension cultures containing IL3. These cultures did not show the effects of IL3's strong induction of proliferation and differentiation (high cell number and exhausted medium); instead, low cell number at the end of the culture period resulted in persistence of cells in the context of cell death. These observations led to the hypothesis that PvFRIL acts in a dominant manner to preserve progenitor viability and prevent proliferation and differentiation.
Moore
J G
JG
ImClone Systems Incorprated, New York, New York 10014, USA. jmoore@phylogix.com
Fuchs
C A
CA
Hata
Y S
YS
Hicklin
D J
DJ
Colucci
G
G
Chrispeels
M J
MJ
Feldman
M
M
D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002470:Cell Survival; D017077:Culture Media, Conditioned; D007887:Fabaceae / Q000737:chemistry*; D005312:Fetal Blood; D006801:Humans; D007377:Interleukin-3 / Q000037:antagonists & inhibitors; D007457:Iodine Radioisotopes; D037102:Lectins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor; D037241:Mannose-Binding Lectins; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000276:immunology; D037121:Plant Lectins; D010946:Plants, Medicinal; D011485:Protein Binding; D021382:Protein Sorting Signals; D012639:Seeds / Q000737:chemistry; D014162:Transfection
D018952:Antigens, CD34; D017077:Culture Media, Conditioned; C117675:FRIL protein, Dolichos lablab; D007377:Interleukin-3; D007457:Iodine Radioisotopes; D037102:Lectins; D037241:Mannose-Binding Lectins; D037121:Plant Lectins; D021382:Protein Sorting Signals; D016173:Macrophage Colony-Stimulating Factor