Imaging Tc-99m-labeled FGF-1 targeting in rats.
Recombinant human acidic fibroblast growth factor (FGF-1) was radiolabeled with (99m)Tc by the HYNIC method. The (99m)Tc-FGF-1 retained its representative molecular mass, heparin affinity, cellular binding to both low (Kd = 9.5 nM) and high (Kd = 125 pM) affinity sites, and mitogenic activity. Gamma camera imaging after intravenous dosing in rats confirmed high liver and kidney binding. Heparin significantly decreased (99m)Tc-FGF-1 liver uptake and increased urinary excretion. These studies illustrate a new method for imaging FGF-1 targeting under various conditions.
Division of Nuclear Medicine, Department of Radiology, University of Alabama, Birmingham, USA. kurtzinn@uab.edu
K R
KR
Zinn
S
S
Kelpke
T R
TR
Chaudhuri
T
T
Sugg
J M
JM
Mountz
J A
JA
Thompson
D000818:Animals; D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2 / Q000493:pharmacokinetics*; D006493:Heparin / Q000494:pharmacology; D007553:Isotope Labeling; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013667:Technetium; D014018:Tissue Distribution
D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D013667:Technetium; D006493:Heparin