Effect of hypothermic pulmonary artery flushing on capillary filtration coefficient.
BACKGROUND
We previously demonstrated that surfactant dilution and inhibition occur immediately after pulmonary artery flushing with hypothermic modified Euro-Collins solution. Consequently, we speculated that increased capillary permeability contributed to these surfactant changes. To test this hypothesis, we evaluated the effects of hypothermic pulmonary artery flushing on the pulmonary capillary filtration coefficient (Kfc), and additionally performed a biochemical analysis of surfactant.


METHODS
We used a murine isolated, perfused lung model to measure the pulmonary capillary filtration coefficient and hemodynamic parameters, to determine the wet to dry weight ratio, and to evaluate surfactant by biochemical analysis of lung lavage fluid. We defined three study groups. In group I (controls), we harvested lungs without hypothermic pulmonary artery flushing, and measured Kfc immediately. In group II (in situ flush), we harvested lungs after hypothermic pulmonary artery flushing with modified Euro-Collins solution, and then measured Kfc. Experiments in groups I and II were designed to evaluate persistent changes in Kfc after pulmonary artery flushing. In group III (ex vivo flush), we flushed lungs ex vivo to evaluate transient changes in Kfc during hypothermic pulmonary artery flushing.


RESULTS
Groups I and II did not differ significantly in capillary filtration coefficient and hemodynamics. Group II showed significant alterations on biochemical surfactant analysis and a significant increase in wet-to-dry weight ratio, when compared with group I. In group III, we observed a significant transient increase in capillary filtration coefficient during pulmonary artery flushing.


CONCLUSIONS
Hypothermic pulmonary artery flushing transiently increases the capillary filtration coefficient, leads to an increase in the wet to dry weight ratio, and induces biochemical surfactant changes. These findings could be explained by the effects of hypothermic modified Euro-Collins solution on pulmonary capillary permeability.
Andrade
R S
RS
Division of Thoracic and Cardiovascular Surgery, University of Minnesota, Minneapolis 55455, USA.
Wangensteen
O D
OD
Jo
J K
JK
Tsai
M Y
MY
Bolman
R M
RM
D000403:Airway Resistance; D000818:Animals; D002196:Capillaries / Q000502:physiology; D002463:Cell Membrane Permeability; D005374:Filtration / Q000379:methods; D006439:Hemodynamics; D006982:Hypertonic Solutions / Q000494:pharmacology; D007035:Hypothermia / Q000503:physiopathology; D016040:Lung Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions; D009929:Organ Size; D010713:Phosphatidylcholines / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011651:Pulmonary Artery / Q000166:cytology; D011663:Pulmonary Surfactants / Q000037:antagonists & inhibitors; D051381:Rats; D011917:Rats, Inbred Lew; D013109:Sphingomyelins / Q000032:analysis
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions; D010713:Phosphatidylcholines; D011506:Proteins; D011663:Pulmonary Surfactants; D013109:Sphingomyelins