[Effect of antioxidants on radical-initiated peroxidation of retinal homogenate].
OBJECTIVE
To compare the effect of antioxidants on radical-initiated peroxidation of retinal homogenate.


METHODS
Lipid peroxides in bovine retinal homogenate were induced by 5 mM FeNO3 (Fe), 25 mM 2, 2'-azobis(2,4'-dimethylvaleronitrile) (lipid-soluble, AMVN) or 50 mM 2,2'-azobis (2-amidinoprpane) dihydrochloride (water-soluble, AAPH) and the preventive effects of antioxidants were measured. Phosphatidylcholine hydroperoxide (PC-OOH) was analyzed with high performance liquid chromatography (HPLC) as the endpoint biomarker.


CONCLUSIONS
Troglitazone, an oral hypoglycemic agent, inhibited PC-OOH production by Fe and AMVN. Therefore, it may be effective for protecting against oxidative stress on the inner surface plasma membranes and subcellular organelle. Quercetin has radical scavenging effects on both sides of the membrane, because it prevents PC-OOH production by AMVN or AAPH. These results demonstrate the usefulness of an in vitro screening test that can accurately and rapidly determine the capacity of an antioxidant against lipid peroxidation or oxidative stress.
Suzuki
K
K
Department of Ophthalmology, Showa University School of Medicine, Tokyo, Japan.
Ueda
T
T
Ueda
T
T
Yamamoto
Y
Y
Chida
M
M
Matsuishi
M
M
Kan
K
K
Higa
A
A
Armstrong
D
D
Yasuhara
H
H
Koide
R
R
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002417:Cattle; D002839:Chromans / Q000494:pharmacology; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D018384:Oxidative Stress; D011794:Quercetin / Q000494:pharmacology; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D013844:Thiazoles / Q000494:pharmacology; D045162:Thiazolidinediones; D000077288:Troglitazone
D000975:Antioxidants; D002839:Chromans; D013844:Thiazoles; D045162:Thiazolidinediones; D011794:Quercetin; D000077288:Troglitazone