Complementation analysis in Fanconi anemia: assignment of the reference FA-H patient to group A.
Fanconi anemia (FA) is an autosomal recessive disorder with diverse clinical symptoms and extensive genetic heterogeneity. Of eight FA genes that have been implicated on the basis of complementation studies, four have been identified and two have been mapped to different loci; the status of the genes supposed to be defective in groups B and H is uncertain. Here we present evidence indicating that the patient who has been the sole representative of the eighth complementation group (FA-H) in fact belongs to group FA-A. Previous exclusion from group A was apparently based on phenotypic reversion to wild-type rather than on genuine complementation in fusion hybrids. To avoid the pitfall of reversion, future assignment of patients with FA to new complementation groups should conform with more-stringent criteria. A new group should be based on at least two patients with FA whose cell lines are excluded from all known groups and that fail to complement each other in fusion hybrids, or, if only one such cell line were available, on a new complementing gene that carries pathogenic mutations in this cell line. On the basis of these criteria, the current number of complementation groups in FA is seven.
Department of Clinical Genetics and Human Genetics, Free University Medical Center, NL-1081 BT Amsterdam, The Netherlands. h.joenje. humgen@med.vu.nl
H
H
Joenje
M
M
Levitus
Q
Q
Waisfisz
A
A
D'Andrea
I
I
Garcia-Higuera
T
T
Pearson
C G
CG
van Berkel
M A
MA
Rooimans
N
N
Morgan
C G
CG
Mathew
F
F
Arwert
D000483:Alleles; D002459:Cell Fusion; D002460:Cell Line; D005199:Fanconi Anemia / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology; D005808:Genes, Recessive / Q000235:genetics; D005816:Genetic Complementation Test; D005838:Genotype; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006967:Hypersensitivity / Q000235:genetics; D020128:Inhibitory Concentration 50; D016685:Mitomycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D010641:Phenotype
D016685:Mitomycin