Arylamine N-acetyltransferase activity and 2 aminofluorene-DNA adducts formation in rat glial tumor cells.
Arylamine N-acetylation capacity by the N-acetyltransferase (NAT) may be an important causative factor in the initiation of cancer. Arylamine-DNA adducts formation have been correlated with the carcinogenic effect of heterocyclic aromatic amines. NAT activity in rat glial tumor cells was measured by high performance liquid chromatography (HPLC) using 2-aminofluorene (2-AF) and p-aminobenzoic acid (PABA) as substrares. 2-AF-DNA adducts formation in rat glial tumor cells was investigated by gamma-[32p]-dATP and HPLC using 2-aminofluorene as substrates. The activities (Mean +/- SD) of NAT in rat glial cells was 1.08 +/- 0.18 nmol/min/mg protein for the acetylation of 2-aminofluorene (n = 12), and 0.96 +/- 0.16 nmol/min/mg protein for the acetylation of p-aminobenzoic acid (n = 12). 2-AF-DNA adducts formation in rat glial tumor cells with 30 microM and 60 microM AF were 0.48 +/- 0.16 and 0.70 +/- 0.12 pmol/mg DNA, respectively. The results indicate that NAT was present in rat glial tumor cells, activating AF to become a metabolite able to bind covalently with DNA to form 2-AF-DNA.
Hung
C F
CF
Department of Pediatrics, Jen-Ai Hospital, Tali, Taichung, Taiwan.
Wong
K T
KT
Tsai
C I
CI
Lin
C S
CS
Chung
J G
JG
D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000032:analysis*; D001932:Brain Neoplasms / Q000737:chemistry*; D002471:Cell Transformation, Neoplastic / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D018736:DNA Adducts / Q000032:analysis*; D005449:Fluorenes / Q000032:analysis*; D005910:Glioma / Q000737:chemistry*; D009153:Mutagens / Q000032:analysis*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000737:chemistry*
D018736:DNA Adducts; D005449:Fluorenes; D009153:Mutagens; C012177:2-aminofluorene; D001191:Arylamine N-Acetyltransferase