[Deletion mutants of the natriuretic peptide receptor type B: expression of cDNA, purification and characteristics of proteins].
The C type natriuretic peptide (CNP) is a peptide hormone stimulating vasorelaxation and inhibiting cell proliferation. CNP activates the type B natriuretic peptide receptor (NPR-B), known as the guanylate cyclase B membrane enzyme, which results in the cGMP release. To study functional properties of NPR-B, its gene fragments were expressed in methylotrophic yeasts Pichia pastoris. Conditions were found providing for secretion of functionally active recombinant proteins NPR-Bs and NPR-B1 into the cultural medium in a yield of 25 mg/ml culture. Their specific activity was 0.97 and 0.93 mumol cGMP min-1 mg-1 protein, respectively. It was shown that NPR-B belongs to the family of Ser/Thr protein kinases and can be autophosphorylated at the serine residues.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. solov@ibch.siobc.ras.ru
O V
OV
Solov'eva
D L
DL
Kakuev
N V
NV
Gibanova
V M
VM
Lipkin
D001483:Base Sequence; D006152:Cyclic GMP / Q000378:metabolism; D017931:DNA Primers; D018076:DNA, Complementary; D006162:Guanylate Cyclase; D009154:Mutation; D010843:Pichia / Q000235:genetics; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000099242:Atrial Natriuretic Factor Receptor B
D006152:Cyclic GMP; D017931:DNA Primers; D018076:DNA, Complementary; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B