cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana.
To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography. Most differential gene fragments were derived from P. parasitica.
The Sainsbury Laboratory, John Innes Centre, Norwich, UK.
E A
EA
van der Biezen
H
H
Juwana
J E
JE
Parker
J D
JD
Jones
D017360:Arabidopsis / Q000382:microbiology*; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D009868:Oomycetes / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary