[Dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E and their clinical significance].
OBJECTIVE
To study the dynamics of anti-HEV ORF2, ORF3, IgM and IgG in serial sera of patients with hepatitis E.


METHODS
Anti-HEV was detected in serial sera of 52 patients, by an enzyme-linked immunosorbent assay (EIA) based on two synthetic peptides separated or combined from open reading frame (ORF) 2 and 3 of HEV genome.


RESULTS
Both anti-ORF2 and anti-ORF3 in serum were at the highest levels in early acute phase of the disease, and declined gradually with the course, especially anti-ORF3. The sensitivity of the EIA using both ORF2 and ORF3 peptides was higher than that of either alone. The positive rates of anti-HEV IgM and anti-HEV IgG were separately 71.1% (32/45) and 97.8% (44/45) within 15 days after onset, then decreased more dramatically in IgM than in IgG with the course.


CONCLUSIONS
Both ORF2 and ORF3 proteins should be used for the development of anti-HEV EIA kit. It seems to be more specific in anti-HEV IgM and more sensitive in anti-HEV IgG as a diagnostic marker of hepatitis E.
Department of Infectious Diseases, First Hospital of Zhejiang Medical University, Hangzhou.
B
B
Ruan
H
H
Zhuang
Y
Y
Ma
D000293:Adolescent; D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006508:Hepatitis Antibodies / Q000097:blood* / Q000235:genetics; D016751:Hepatitis E / Q000276:immunology* / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D014764:Viral Proteins / Q000276:immunology*
D006508:Hepatitis Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C400971:ORF2 protein, Hepatitis E virus; C400973:ORF3 protein, Hepatitis E virus; D014764:Viral Proteins