Microisolated mouse osteoclasts express VIP-1 and PACAP receptors.
Skeletal tissue contains a network of nerve fibers expressing several neuropeptides, including vasoactive intestinal peptide (VIP) and the related peptide pituitary adenylate cyclase activating peptide (PACAP). These peptides have been demonstrated to regulate osteoclast formation and osteoclast activity. Using atomic force microscopy and by analysing changes of the intracellular calcium concentrations, we have recently demonstrated that multinucleated rat osteoclasts have cell membrane binding sites recognising VIP and PACAP. In the present study, we have further studied the expression of VIP receptor subtypes in mouse bone marrow cultures and isolated osteoclasts. A micromanipulation technique was used to isolate pure populations of osteoclasts formed in PTH-stimulated mouse bone marrow cultures. By reverse transcriptase polymerase chain reaction (RT-PCR), we studied the expression of mRNA for VIP-1, VIP-2, and PACAP receptors. The purity of the microisolated osteoclasts was determined by studying the expression of specific mRNA associated with the phenotypic trait of osteoclasts or osteoblasts/stromal cells. In this study, we show that mouse osteoclasts express VIP-1 and PACAP, but not VIP-2, receptor mRNA.
Ransjö
M
M
Department of Oral Cell Biology, Umeå University, Umeå, Sweden. maria.ransjo@odont.umu.se
Lie
A
A
Mukohyama
H
H
Lundberg
P
P
Lerner
U H
UH
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D008846:Micromanipulation; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D011979:Receptors, Pituitary Hormone / Q000096:biosynthesis* / Q000235:genetics; D018005:Receptors, Vasoactive Intestinal Peptide / Q000096:biosynthesis* / Q000235:genetics; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D012680:Sensitivity and Specificity; D000071681:Tartrate-Resistant Acid Phosphatase
D000943:Antigens, Differentiation; D007527:Isoenzymes; D010281:Parathyroid Hormone; D012333:RNA, Messenger; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D011979:Receptors, Pituitary Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D051239:Receptors, Vasoactive Intestinal Peptide, Type II; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase