A beta fibrillogenesis: kinetic parameters for fibril formation from congo red binding.
Using Scatchard analysis, we have formulated as a function of time and pH the relationship between the binding of Congo red to Alzheimer's beta-amyloid and the aggregation number (i.e., monomer concentration within fibrils) as defined by nucleation-dependent self-assembly. This provides a basis on which to determine the kinetic parameters for fibril formation from the observed concentration of bound Congo red.
Inouye
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Department of Biology, Boston College, Chestnut Hill, Massachusetts, 02467-3811, USA.
Kirschner
D A
DA
D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D003224:Congo Red / Q000737:chemistry / Q000378:metabolism; D019281:Dimerization; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D013997:Time Factors
D016229:Amyloid beta-Peptides; D003224:Congo Red