Subunits of neurosteroid sulfatase from bovine brain.
We have purified the neurosteroid sulfatase (NSS) from Triton X-100 solubilized microsomes of bovine brain about 100-fold. The purified enzyme is composed of two catalytic units (MW: 57 kDa) and two regulatory units (MW: 38 kDa), making it an alpha(2)beta(2) heterotetramer, whose apparent molecular weight was 180 kDa by gel filtration in the presence of Triton X-100.
Park
I H
IH
Brain Disease Research Center, School of Medicine, Ajou University, Suwon, South Korea.
Han
B K
BK
Baek
J H
JH
Ryu
Y W
YW
Park
Y H
YH
Jo
D H
DH
D000818:Animals; D001192:Arylsulfatases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008861:Microsomes / Q000201:enzymology; D043266:Steryl-Sulfatase
D001192:Arylsulfatases; D043266:Steryl-Sulfatase