Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae.
Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.
Kusakabe
T
T
Laboratory of Silkworm Sciences, Faculty of Agriculture, Kyusyu University, Fukuoka, Japan. kusakabe@agr.kyushu-u.ac.jp
Sugimoto
Y
Y
Hirota
Y
Y
Toné
S
S
Kawaguchi
Y
Y
Koga
K
K
Ohyama
T
T
D000818:Animals; D001236:Aspergillus oryzae / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D022004:Chromosome Structures / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008322:Mammals / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D004271:DNA, Fungal