Ratiometric oxygen sensing: detection of dual-emission ratio through a single emission filter.
A new method and device for the ratiometric measurement of oxygen concentration are presented. They are based on the use of a dual-emission oxygen-sensitive dye. The method allows the exclusion of the influence of emission overlap. The detection of the dual-emission ratio is performed using a single long-pass emission filter. The device described is simpler than the widely used lifetime instruments and could easily be a stand-alone low-cost instrument.
Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore 21201, USA.
Y
Y
Kostov
P
P
Harms
R S
RS
Pilato
G
G
Rao
D004396:Coloring Agents; D005336:Fiber Optic Technology; D049449:Luminescence; D010100:Oxygen / Q000032:analysis*
D004396:Coloring Agents; D010100:Oxygen