The effects of sarin-like and soman-like organophosphorus agents on MAPK and JNK in rat brains.
One sarin-like and one soman-like organophosphorus agent [bis(isopropyl methyl)phosphonate, BIMP and bis(pinacolyl methyl)phosphonate, BPMP] were injected intravenously (iv) in rats. An increase in the tyrosine phosphorylation of several proteins in the cytosol fraction of the brain was observed. Activation of c-Jun N-terminal kinase (JNK) and slight activation of mitogen-activated protein kinase (MAPK) in the cytosol were also observed. The activation of these enzymes may be related to the high toxicity of these nerve agents.
Niijima
H
H
Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113-0033, Tokyo, Japan.
Nagao
M
M
Nakajima
M
M
Takatori
T
T
Iwasa
M
M
Maeno
Y
Y
Koyama
H
H
Isobe
I
I
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002619:Chemical Warfare Agents / Q000009:adverse effects*; D002800:Cholinesterase Inhibitors / Q000009:adverse effects*; D004396:Coloring Agents; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004164:Diphosphonates / Q000009:adverse effects*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D048031:JNK Mitogen-Activated Protein Kinases; D008163:Luminescent Measurements; D048369:MAP Kinase Kinase 1; D008297:Male; D020929:Mitogen-Activated Protein Kinase Kinases / Q000187:drug effects*; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012524:Sarin / Q000009:adverse effects*; D012999:Soman / Q000009:adverse effects* / Q000031:analogs & derivatives*; D013912:Threonine / Q000187:drug effects / Q000378:metabolism; D014443:Tyrosine / Q000187:drug effects / Q000378:metabolism
D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D004396:Coloring Agents; D004164:Diphosphonates; C109992:bis(isopropyl methyl)phosphonate; D013912:Threonine; D014443:Tyrosine; C046573:pinacolyl methylphosphonic acid; D012999:Soman; D012524:Sarin; D017346:Protein Serine-Threonine Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048369:MAP Kinase Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases