[Mechanism of IL-6 secretion in bone marrow cells from multiple myeloma patients].
OBJECTIVE
To explore the mechanism of interleukin-6(IL-6) secretion in bone marrow cells from patients with multiple myeloma(MM).


METHODS
Expression of IL-6 in bone marrow mononuclear cells(BMMNC) from MM patients(n = 16) and controls(n = 19) was detected by using double labelled immunofluorescence.


RESULTS
In MM group, the positive rates of IL-6 expression were 15.4% +/- 6.5% in BMMNC, 7.9% +/- 2.9% in myeloma cells, and 4.5% +/- 1.2% in stromal cells, while in controls the positive rates were 4.6% +/- 2.3% in BMMNC, 2.6% +/- 1.1% in stromal cells and 0.7% +/- 0.1% in plasma cells, respectively. The positive rates in MM group were all significantly higher than those in controls(P < 0.001). The cytoplasmic IgG+ and vWF+ cells percentages in BMMNC were also significantly higher in MM group than those in controls.


CONCLUSIONS
Elevated IL-6 in MM patients was secreted by both myeloma cells and stromal cells, and the mechanism of both autocrine and paracrine may be involved in pathogeneses of multiple myeloma.
Liang
Z
Z
Changzheng Hospital, Second Military Medical University, Shanghai.
Hou
J
J
Ding
S
S
D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000276:immunology* / Q000473:pathology
D015850:Interleukin-6