Atrial natriuretic peptide modulates N-methyl-D-aspartate-induced hyperexcitability in ethanol-dependent mice.
The role of central nervous atrial natriuretic peptide was investigated for behavioral hyperexcitability in alcohol-dependent mice. Mice were made tolerant to and dependent on ethanol with an ethanol-liquid diet for 14 days. Five hours after withdrawal from ethanol, withdrawal symptoms were analyzed by scoring handling-induced convulsions. N-methyl-D-aspartate (NMDA) induced behavioral seizures in a dose-dependent manner, an effect which was more intensive during the ethanol withdrawal period than in alcohol-naive animals. Intracerebroventricular (i.c.v.) injections of alpha-atrial natriuretic peptide (atrial natriuretic peptide) dose-dependently inhibited, whereas injection of an antiserum against atrial natriuretic peptide potentiated, the seizure-inducing effect of NMDA in ethanol-dependent mice. The main conclusion is that central nervous atrial natriuretic peptide plays a modulatory role in behavioral hyperexcitability during alcohol withdrawal.
Central Laboratory, Markusovszky Teaching Hospital, Markusovszky St. 3., H-9701, Szombathely, Hungary. glkovacs@mail.matav.hu
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D000818:Animals; D009320:Atrial Natriuretic Factor / Q000276:immunology / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D007106:Immune Sera / Q000009:adverse effects; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D016202:N-Methylaspartate / Q000009:adverse effects*; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control*; D019966:Substance-Related Disorders
D007106:Immune Sera; D000431:Ethanol; D016202:N-Methylaspartate; D009320:Atrial Natriuretic Factor