Liposomes as protective agents of stratum corneum against octyl glucoside: a study based on high-resolution, low-temperature scanning electron microscopy.
The ability of phosphatidylcholine (PC) liposomes to protect pig stratum corneum (SC) against the action of the nonionic surfactant octyl glucoside (OG) was investigated "in vitro" using double-layer coating for high-resolution, low-temperature scanning electron microscopy. This technique has been useful in preventing drying artifacts in the study of biological materials. The treatment of SC with OG led to a perturbation mainly in the corneocytes. However, the incubation of the tissue with liposomes prior to the OG treatment resulted in a progressive decrease in these perturbations and, consequently, in the progressive protection of the SC against the action of the surfactant.
Departmento de Tensioactivos, Centro de Investigación y Desarrollo, Consejo Superior de Investigaciones Científicas C/ Jordi Girona, 18-26, 08034 Barcelona, Spain.
O
O
López
M
M
Cócera
P
P
Walther
E
E
Wehrli
L
L
Coderch
J L
JL
Parra
A
A
de la Maza
D000818:Animals; D003902:Detergents / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects*; D005614:Freeze Fracturing; D005960:Glucosides / Q000494:pharmacology*; D008081:Liposomes / Q000494:pharmacology*; D008854:Microscopy, Electron; D010713:Phosphatidylcholines / Q000494:pharmacology*; D013552:Swine
D003902:Detergents; D005960:Glucosides; D008081:Liposomes; D010713:Phosphatidylcholines; C018619:octyl-beta-D-glucoside