Different reticulocyte volume in diabetes mellitus patients with and without hypercholesterolemia and/or hypertriglyceridemia.
Red cells and reticulocytes from patients with diabetes mellitus (DM) were analysed using laser and computer technology of H*3 hematology analyzer. Four groups of diabetes mellitus patients: diabetes with normolipemia (DM) (n = 12) and DM patients with excess triglyceride (DM-T) (n = 7) or cholesterol (DM-C) (n = 21) or both (DM-TC) (n = 21) were studied. Mean corpuscular volume of mature red cells (Mean +/- SD = 93.6 +/- 5.5 fl) and reticulocyte (119.1 +/- 12.3 fl) of patients with DM-T was not significantly increased from normal (red cell, 90.0 +/- 3.5 fl; reticulocyte, 115.2 +/- 7.3 fl). Plasma triglyceride levels had no significant correlation with red cell MCV, reticulocyte MCV and %Hb A1c. This suggests that high triglyceride levels in DM are not dose-dependent in producing increased MCV of red cells and reticulocytes. Comparing between DM-T and DM-C, red cells and reticulocytes from DM-C patients had significantly decreased MCV (red cell, 85.5 +/- 6.1 fL; reticulocyte, 103.8 +/- 7.4 fL). Plasma cholesterol levels were inversely correlated with MCV of red cells (r = -0.377, p = 0.003) and also MCV of reticulocytes (r = -0.418, p = 0.001). In addition, cholesterol levels showed considerable correlation with Hb A1c (r = 0.572, p = 0.004). The red cell volume change in DM-C patients may be partly due to the shift in balance of cholesterol exchange between red cell membranes and serum lipoproteins.
Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
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Bunyaratvej
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Komindr
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Wisedpanichkij
D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood*; D004909:Erythrocyte Indices; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D015228:Hypertriglyceridemia / Q000097:blood*; D008297:Male; D011336:Probability; D012016:Reference Values; D017701:Reticulocyte Count; D012156:Reticulocytes / Q000502:physiology*; D012680:Sensitivity and Specificity