Enantioselective supercritical fluid chromatography using ristocetin A chiral stationary phases.
Racemic mixtures of five acidic drugs have been successfully separated by supercritical fluid chromatography (SFC) using macrocyclic antibiotic chiral stationary phases (CSPs). A ristocetin A CSP has been prepared 'in-house' and effectively applied in packed capillary SFC to separate the enantiomers of dichlorprop (R(s) = 1.4), ketoprofen (R(s) = 0.9) and warfarin (R(s) = 0.9). The commercial ristocetin A CSP (Chirobiotic R) was subsequently studied in packed column SFC with similar results where the enantiomers of warfarin (R(s) = 2.2), coumachlor (R(s) = 2.5) and thalidomide (R(s) = 0.6) were separated. Interestingly, differences were observed between the two differently immobilised CSPs where the enantiomers of dichlorprop and ketoprofen, which were separated on the 'in-house' CSP, could not be separated on the commercial phase.
Svensson
L A
LA
Analytical Development, AstraZeneca R&D Mölndal, Sweden.
Owens
P K
PK
D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives / Q000032:analysis; D000779:Anesthetics, Local / Q000032:analysis; D000900:Anti-Bacterial Agents; D002045:Bupivacaine / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis; D005069:Evaluation Studies as Topic; D007660:Ketoprofen / Q000032:analysis; D008790:Metoprolol / Q000032:analysis; D011433:Propranolol / Q000032:analysis; D011933:Reagent Kits, Diagnostic; D012310:Ristocetin; D013237:Stereoisomerism; D013792:Thalidomide / Q000032:analysis; D014859:Warfarin / Q000032:analysis
D000779:Anesthetics, Local; D000900:Anti-Bacterial Agents; D003374:Coumarins; D011933:Reagent Kits, Diagnostic; D012310:Ristocetin; D015084:2,4-Dichlorophenoxyacetic Acid; D013792:Thalidomide; D014859:Warfarin; D007660:Ketoprofen; D011433:Propranolol; D008790:Metoprolol; C010571:dichlorprop; C412550:coumachlor; D002045:Bupivacaine