A discussion of anti-Aspergillus niger glucose oxidase monoclonal antibody reactivity to red blood cells of several species.
We observed that mouse spleen cells from rosettes with autologous red blood cells (RBCs) and that rosette-formation was suppressed by anti-Aspergillus niger glucose oxidase monoclonal antibody (mAb). In the present study, we investigated whether RBCs of species besides mice have the structure recognized by anti-A. niger glucose oxidase mAb by using rosette-formation and complement-mediated hemolysis. Lysates of monkey and human RBCs did not suppress rosette-formation whereas autologous (mouse), rat and sheep RBC lysates partially suppressed rosette-formation. Those lysates exerted their suppressive activity after they had been treated at 56 degrees C for 30 min. A. niger glucose oxidase also suppressed rosette-formation with or without treatment at 56 degrees C for 30 min. Alternatively, anti-A. niger glucose oxidase mAb lysed mouse, rat and sheep RBCs but not human RBCs with complement. These findings suggest that the cell surfaces of mouse, rat and sheep RBCs have a structure which can be recognized by anti-A. niger glucose oxidase mAb while the cell surfaces of monkey and human RBCs do not.
Kojima
T
T
First Department of Surgery, Aichi Medical University, Japan.
Nagata
H
H
Tokunaga
Y
Y
Sano
M
M
Sasanabe
R
R
Suzumura
K
K
Kanemitsu
T
T
Naruse
T
T
D000818:Animals; D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D001234:Aspergillus niger / Q000201:enzymology* / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D005949:Glucose Oxidase / Q000276:immunology*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D012397:Rosette Formation; D012756:Sheep; D013045:Species Specificity
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D005949:Glucose Oxidase