Expression of neuropeptide Y and neuropeptide Y Y1 receptors and neuronal markers following axotomy in the rat spinal cord and gracile nucleus.
Using immunocytochemistry, the effects of denervation on the expressions of the neuropeptide Y Y1 receptor, neuropeptide Y and neuronal markers were investigated in the lumbar spinal cord of the rat. Ten, 17 and 24 days after unilateral sciatic nerve section, the distribution of the neuropeptide Y Y1 receptor was seen in lamina II in the ipsilateral and contralateral side of the lumbar spinal cord and gracile nucleus, whereas neuropeptide Y immunoreactivity located strongly in laminae I-II and moderately in laminae III-IV in the ipsilateral side. Denervation, following section of the sciatic nerve, resulted in no change in the distribution of the neuropeptide Y Y1 receptor in the spinal cord. This suggests that the neuropeptide Y that is expressed in myelinated afferents following nerve section does not affect the expression of this receptor. This is particularly apparent in the gracile nucleus which shows clear neuropeptide Y staining following sciatic nerve section and no expression of the neuropeptide Y Y1 receptor.
Nazli
M
M
Department of Histology-Embryology, Faculty of Veterinary Medicine, University of Kafkas, Kars, Turkey.
Morris
R
R
D000818:Animals; D019771:Axotomy; D003714:Denervation; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000378:metabolism*
D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor