[The effect of Epstein-Barr virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cells].
OBJECTIVE
To investigate the effect of EB virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cell.


METHODS
Expression vector for BHRF1 was constructed and transfected into nsaopharyngeal carcinoma cell line, CNE2. The alteration of biologic behaviour of the transfected cells was tested after 60Co radiation.


RESULTS
BHRF1 expression could inhibit the expression of proliferative cell nuclear antigen, redistribute cell cycle, decrease cell sensitivity to radiation, and enhance their survival ability.


CONCLUSIONS
It is suggested that BHRF1 expression can increase the apoptotic resistance induced by radiation.
Department of Otolaryngology, Changhai Hospital, Second Military Medical University.
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Huang
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Pan
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Yu
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D015870:Gene Expression; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis*
C083278:BHRF1 protein, Human herpesvirus 4; D003037:Cobalt Radioisotopes; D018809:Proliferating Cell Nuclear Antigen; D014764:Viral Proteins