[Immunohistochemical study of p53, c-erbB-2 and nm23 proteins in human gastric cancer].
OBJECTIVE
To evaluate p53, c-erbB-2 and nm23 oncoproteins in diagnosis of gastric cancer.


METHODS
The expression of p53, c-erbB-2 and nm23 oncoproteins was detected immunohistochemically in 141 surgically resected specimens and 93 endoscopic biopsy specimens of gastric cancer.


RESULTS
The positive rate of p53, c-erbB-2 and nm23 immunoreactivity was 45.8-56.3%, 18.3-31.2% and 72.9%-81.3% respectively. No immunoreactivity was observed in the tumor-adjacent normal mucosa and non-tumor biopsy specimens. The presence of c-erbB-2 was correlated with the size of tumor, grade of tumor differentiation and Lanren classification. Patients with c-erbB-2 positive tumor had lower 5-year survival rate. The expression of nm23 was negatively correlated with the depth of invasion, and lymphnode metastasis. The positive rates of p53, c-erbB-2 and nm23 had no statistical difference between biopsy and resected specimens.


CONCLUSIONS
Determination of p53, c-erbB-2 and nm23 oncoproteins immunohistochemically might be useful in the diagnosis and differential diagnosis, clinical staging, and predicting prognosis of gastric cancer.
Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University.
Z
Z
Wei
Y
Y
Wu
Z
Z
Zhai
D000230:Adenocarcinoma / Q000175:diagnosis / Q000473:pathology; D000231:Adenocarcinoma, Papillary / Q000175:diagnosis / Q000473:pathology; D018279:Carcinoma, Signet Ring Cell / Q000175:diagnosis / Q000473:pathology; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D020559:Monomeric GTP-Binding Proteins / Q000032:analysis*; D054778:NM23 Nucleoside Diphosphate Kinases; D009367:Neoplasm Staging; D009701:Nucleoside-Diphosphate Kinase; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000473:pathology; D014157:Transcription Factors / Q000032:analysis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D018719:Erb-b2 Receptor Tyrosine Kinases; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C517938:NME1 protein, human