[Detection of CBF beta-MYH11 fusion transcript and inv(16)(p13;q22) in acute myelomonocytic leukemia(M4) by RT-PCR and FISH].
OBJECTIVE
To study the clinical significance of inv(16) and CBF beta-MYH11 fusion gene in the diagnosis and prognosis for M4Eo.


METHODS
CBF beta-MYH11 fusion transcripts and inv (16) were analyzed by reverse-transcriptase polymerase chain reaction(RT-PCR) and fluorescence in situ hybridization(FISH), respectively, in acute myelomonocytic leukemia(M4) with or without eosinophilia.


RESULTS
In fifteen cases tested, one case of M4Eo and one of 9 M4 without eosinophilia were found to have CBF beta-MYH11 fusion transcript. Follow-up of the M4Eo patient showed residual PCR positivity 2 months after complete remission.


CONCLUSIONS
The data suggest that screening by both RT-PCR and FISH should be performed in all AML-M4 regardless of morphologic features to allow accurate diagnosis and prognosis of M4 patients.
Shanghai Institute of Hematology, Shanghai Second Medical University.
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D000293:Adolescent; D000328:Adult; D000368:Aged; D019677:Artificial Gene Fusion; D002648:Child; D002675:Child, Preschool; D007446:Chromosome Inversion; D002885:Chromosomes, Human, Pair 16; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015479:Leukemia, Myelomonocytic, Acute / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
C095514:CBFbeta-MYH11 fusion protein; D015514:Oncogene Proteins, Fusion