[Study on effects of new retinoids in combination with IFN-gamma on proliferation and differentiation of U937 cell and its mechanism].
OBJECTIVE
To illustrate the effects of new retinoids in combination with IFN-gamma on the proliferation and differentiation of U937 cell and its mechanism.


METHODS
Effects of new retinoids R9158, YS904012 in combination with IFN-gamma on the proliferation and differentiation of U937 cells were studied by detecting NBT, expression of CD14, CD68 and c-myc, and cell cycle shift.


RESULTS
When the U937 cells had been first treated with new retinoid for 24 hours, and then with IFN-gamma, the differentiation activity could be enhanced, the reverse of the use of two agent in chronology displayed much lower synergistical effect. Retinoids could down regulate c-myc expression and shift the cell population from S phase to G0/G1 phase within 24 hours, and the activity of YS904012 was greater than that of other retinoids.


CONCLUSIONS
New retinoids in combination with IFN-gamma synergistically induced U937 differentiation. Retinoids may play an important role in triggering the differentiation.
Ruijin Hospital, Shanghai Second Medical University.
H
H
Chen
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G
Sun
J
J
Li
D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D012176:Retinoids / Q000494:pharmacology*; D020298:U937 Cells
D016271:Proto-Oncogene Proteins c-myc; D012176:Retinoids; D007371:Interferon-gamma