[Cellular biological mechanism of leukocytosis produced by all-trans retinoic acid: a preliminary study].
OBJECTIVE
To investigate the cellular biological mechanism of leukocytosis produced by all-trans retinoic acid (ATRA).


METHODS
BALB/C mice were fed by ATRA. The effects of ATRA on BALB/C mice hematopoiesis was studied by 3H-TdR, semi-solid progenitor culture, and CFU-S number.


RESULTS
ATRA increased the peripheral blood cells count in vivo. The effect on WBC count was obvious. The peak of the elevation (183% of the control) occurred at the 16th day. Correspondingly, 3H-TdR was increased and CFU-S was enhanced in BM, and the peak of the elevation (190% and 200% of the control, respectively) occurred at the 8th day. The progenitor cell CFU-GM was proliferated and the maximum elevation (215% of control) was observed at the 12th day.


CONCLUSIONS
ATRA could produce leukocytosis in normal BALB/C mice. The effect was possibly related to the proliferation of CFU-GM and CFU-S and to ATRA-induced CSA such as G-CSF in vivo.
Ruijin Hospital, Shanghai.
H
H
Chen
G
G
Sun
J
J
Li
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007964:Leukocytosis / Q000139:chemically induced*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D014212:Tretinoin / Q000009:adverse effects*
D014212:Tretinoin