[Study on gene of YRRM in azoospermia].
OBJECTIVE
To detect the abnormal YRRM gene in azoospermia and to explore the pattern of YRRM gene in Chinese men.


METHODS
With the special primer for YRRM, the YRRM gene was analyzed using the PCR method. Extracting the testis total RNA, we performed the RT-PCR. The part of YRRM gene was sequenced.


RESULTS
No amplification band of YRRM gene was noted in 4 of 74 Chinese men with azoospermia (5.4%). The YRRM gene was obviously expressed in the testis of man and showed a 500 bp cDNA fragment. The cDNA and genomic DNA were different because the intron. We found that Chinese people only have YRRM1 pattern of gene, and the sequence of YRRM gene of Chinese men shows that the composition of the nucleotide is same as YRRM1 gene.


CONCLUSIONS
These cases of abnormal YRRM gene may result in male infertility, YRRM gene and azoospermia is the YRRM1 gene is polymouphase of racial.
Laboratory of Molecular Biology, General Hospital of PLA, Beijing.
Y
Y
Zhao
D
D
Tan
Z
Z
Gu
D000328:Adult; D005838:Genotype; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014998:Y Chromosome