A novel configuration of bioartificial liver support system based on circulating microcarrier culture.
The purpose of this investigation is to initiate a new bioartificial liver support system that utilizes circulating microcarrier cultures in the extracapillary space of a hollow fiber cartridge. The material exchange occurs on the membranes of the hollow fiber. Toxins are metabolized by the circulating cells on the microcarriers driven by a centrifugal pump. We inoculated 2-3 x 10E8 Hep G2 cells on 2.5 grams of Cytodex 3 microcarriers, and allowed them flowing in the extracapillary space of a modified plasma filter. 10% FCS Medium was pumped through the capillaries at different rates. Cells keep morphological integrity and functionality during the circulation. These preliminary results suggest that this configuration of a bioartificial liver support system offers a future investigation.
Institute for Molecular Biology, Nankai University, Tianjin, PR China.
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Shi
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Mitteregger
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Falkenhagen
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YT
Yu
D000418:Albumins / Q000096:biosynthesis; D000641:Ammonia / Q000494:pharmacology; D019149:Bioreactors; D006528:Carcinoma, Hepatocellular; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002470:Cell Survival; D005110:Extracellular Space; D005947:Glucose / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019164:Liver, Artificial; D010477:Perfusion; D014407:Tumor Cells, Cultured
D000418:Albumins; D000641:Ammonia; D007770:L-Lactate Dehydrogenase; D005947:Glucose