In vivo (31)P-MRS assessment of cytosolic [Mg(2+)] in the human skeletal muscle in different metabolic conditions.
Cytosolic free [Mg(2+)] can be assessed in vivo by (31)P-MRS from the chemical shift of beta-ATP. The reliability of in vivo measurements depends on the availability of appropriate in vitro calibration curves obtained by using solutions that mimic the in vivo cytosolic conditions as far as possible. We build a semi-empiric equation that correlates the chemical shift of beta-ATP to free [Mg(2+)] taking into account the amount of Mg(2+) bound to all other ligands in solution. Our experiments resulted in a reliable ten-parameters equation directly usable to assess the cytosolic free [Mg(2+)] of human skeletal muscle at rest, during work and recovery. Our experiments also resulted in a new equation that allows the assessment of cytosolic pH from the chemical shift of Pi taking into account the measured free [Mg(2+)]. To perform simultaneous calculation of free [Mg(2+)] and pH in the skeletal muscle in different metabolic conditions we developed a specific software package available on Internet (http://www.unibo.it/bioclin) together with another program based on the equation previously obtained to calculate cytosolic free [Mg(2+)] in the human brain. The reliability and effectiveness of our equations and software were tested on the calf muscles of healthy volunteers at rest, during work and recovery.
Biochimica Clinica, Dipartimento di Medicina Clinica e Biotecnologia applicata "D Campanacci," Università di Bologna, Italy. iooti@med.unibo.it
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Iotti
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Frassineti
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Alderighi
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Sabatini
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Vacca
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Barbiroli
D000328:Adult; D002138:Calibration; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D020127:Recovery of Function / Q000502:physiology; D015203:Reproducibility of Results; D012146:Rest / Q000502:physiology; D012984:Software; D014937:Work / Q000502:physiology
D010761:Phosphorus Radioisotopes; D008274:Magnesium