Identification and characterization of differentially expressed genes in denervated muscle.
Denervation results in a series of changes in skeletal muscle. To elucidate the molecular basis underlying these changes, it is important to identify the profile of altered gene expression in skeletal muscle following nerve injury. In the present study, we have examined the differentially expressed genes in denervated gastrocnemius muscle using RNA fingerprinting by arbitrarily primed PCR. Eight differentially expressed mRNA transcripts have been identified. A bilateral regulatory profile can be observed for the up-regulated genes in both denervated and contralateral control muscle following unilateral sciatic nerve injury. The temporal expression profiles of the denervation-regulated genes in muscle during development, together with their dependency on nerve activity, suggest potential functional roles following nerve injury in vivo. In particular, the identification of two apoptosis-related genes in denervated muscle provides molecular evidence that the apoptotic process is likely to be involved in the intricate changes that lead to muscle atrophy. Our findings not only allow the identification of novel genes, but also suggest possible functions for some known genes in muscle following nerve injury. Taken together, these findings provide important insights into our understanding of the molecular events in denervated muscle and suggest that the differentially expressed genes may play potential roles during muscle denervation and regeneration.
Tang
H
H
Shanghai Research Center of Life Science and Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, China.
Cheung
W M
WM
Ip
F C
FC
Ip
N Y
NY
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D015152:Blotting, Northern; D002154:Calpain / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D005974:Glutamate-Ammonia Ligase / Q000235:genetics; D008297:Male; D009121:Muscle Denervation; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000201:enzymology; D009124:Muscle Proteins / Q000235:genetics*; D018482:Muscle, Skeletal / Q000166:cytology / Q000294:innervation* / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000235:genetics; D009409:Nerve Crush; D054409:Nicotinamide Phosphoribosyltransferase; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000235:genetics*; D012584:Sciatic Nerve / Q000187:drug effects / Q000293:injuries* / Q000502:physiology; D047992:TNF Receptor-Associated Factor 2; D013779:Tetrodotoxin / Q000494:pharmacology; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
D002352:Carrier Proteins; D016207:Cytokines; D009124:Muscle Proteins; C101338:PSMD2 protein, human; D012333:RNA, Messenger; D047992:TNF Receptor-Associated Factor 2; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D013779:Tetrodotoxin; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human; D002154:Calpain; D018995:Myosin Heavy Chains; D010751:Phosphopyruvate Hydratase; D005974:Glutamate-Ammonia Ligase