In vitro multiplication of Nothapodites foetida (Wight.) Sleumer through seedling explant cultures.
In vitro multiplication of Nothapodites foetida (Wight.) Sleumer was achieved using axenic seedling explant cultures. Isolated nodes (1.0-1.2 cm) and shoot tips (1.0-1.5 cm) cultured in Murashige and Skoog's agar medium containing varying concentrations of TDZ, BA and combinations of 2iP and GA3. Single shoot (0.8-1.2 cm) was regenerated in each culture after 6 weeks. Axillary shoots were then excised and recultured for 8 weeks in medium containing TDZ (0.05 mgL-1) which formed shoots (about 4 in no.; 2 cm) from the basal node. Axillary branches (2) which formed on 60% of these shoots after 10-12 weeks of culture were separated and recultured in the same medium for 8 weeks. Three shoots (0.8-1.0 cm) per culture were regenerated. Shoots of 0.8-1.8 cm length were subcultured on a low cytokinin (0.01 mgL-1 TDZ) regime to induce shoot elongation (2.0-3.5 cm) in 4 weeks. Shoot cuttings were rooted (60%) in the medium containing IBA (1.5 mgL-1). Rooted plantlets established in pots (90%) after hardening resumed normal growth in 3 months.
Plant Biotechnology Division, Tropical Botanic Garden and Research Institute, Thiruvananthapuram, India.
K
K
Satheeshkumar
S
S
Seeni
D001901:Botany / Q000379:methods; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D063245:Plant Development; D010946:Plants, Medicinal / Q000254:growth & development*; D014197:Trees / Q000254:growth & development*
D003470:Culture Media