[The properties of the cellular surface of Yersinia pestis strain EV and its achromogenic variants].
The comparative study of the properties of the surface of vaccine strain Y. pestis EV and its achromogenic variants (AV) differing from the initial strain by decreased immunogenicity and by the morphology of colonies, has been made. The achromogenicity of Y. pestis colonies has been shown to correlate with the loss of the outer membrane protein with a molecular weight 22 kD. The synthesis of this protein is determined by chromosomal genes. AV have been found to have different sensitivity to bacteriophages. The analysis of the electrokinetic potential of Y. pestis EV and its AV has revealed that in the latter have surface charge is considerably greater (1.4- to 1.5-fold). As shown in this study, the hemagglutinating activity of AV with respect to red blood cells of humans with blood group I (O) and guinea pigs is decreased by 1-2 orders and these strains do not agglutinate with sheep red blood cells. The low activity of the initial stage of the phagocytosis of AV by mouse macrophages has been shown. The possible role of the 22 kD proteins as an adhesion factor is discussed.
Research Institute for Plague Control Microbe, Saratov, Russia.
S P
SP
Zadnova
A A
AA
Shcherbakov
I A
IA
Diatlov
L M
LM
Kukleva
O A
OA
Protsenko
N P
NP
Kononov
D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000382:microbiology; D014644:Genetic Variation; D006168:Guinea Pigs; D006801:Humans; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D010587:Phagocytosis; D010931:Plague Vaccine; D012641:Selection, Genetic; D012756:Sheep; D013499:Surface Properties; D014613:Vaccines, Attenuated; D015010:Yersinia pestis / Q000737:chemistry / Q000472:pathogenicity*
D001425:Bacterial Outer Membrane Proteins; D010931:Plague Vaccine; D014613:Vaccines, Attenuated