Determination of neurotoxin 3-N-oxalyl-2,3-diaminopropionic acid and non-protein amino acids in Lathyrus sativus by precolumn derivatization with 1-fluoro-2,4-dinitrobenzene.
A rapid and simple method is presented for determining neuro-excitatory nonprotein amino acid 3-N-oxalyl-2,3-diaminopropionic acid (beta-ODAP) and non-protein amino acids in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid chromatography method (RP HPLC) for the separation of the derivatives in the pmol range is reported. The RP HPLC method and a colorimetric method were compared for measuring ODAP.
Wang
F
F
National Laboratory of Applied Organic Chemistry, Lanzhou University, PR China.
Chen
X
X
Chen
Q
Q
Qin
X
X
Li
Z
Z
D000596:Amino Acids / Q000032:analysis*; D000599:Amino Acids, Diamino / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004139:Dinitrofluorobenzene / Q000737:chemistry*; D009498:Neurotoxins / Q000032:analysis*; D010945:Plants, Edible / Q000737:chemistry*; D010947:Plants, Toxic / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D000599:Amino Acids, Diamino; D009498:Neurotoxins; C100126:oxalyldiaminopropionic acid; D004139:Dinitrofluorobenzene