Dual functions of Streptococcus salivarius urease.
A urease-deficient derivative of Streptococcus salivarius 57.I was constructed by allelic exchange at the ureC locus. The wild-type strain was protected against acid killing through hydrolysis of physiologically relevant concentrations of urea, whereas the mutant was not. Also, S. salivarius could use urea as a source of nitrogen for growth exclusively through a urease-dependent pathway.
Chen
Y Y
YY
Center for Oral Biology and Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA.
Weaver
C A
CA
Burne
R A
RA
D017353:Gene Deletion; D007700:Kinetics; D015183:Restriction Mapping; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014510:Urease / Q000235:genetics / Q000378:metabolism*
D014510:Urease