Vascular endothelial growth pattern of endochondral bone graft in the presence of demineralized intramembranous bone matrix--quantitative analysis.
OBJECTIVE
To determine the timely ingrowth of new blood vessels of composite endochondral (EC) bone and demineralized bone matrix (DBM) prepared from intramembranous (IM) origin (EC-DBM(IM)) and to compare it with EC bone graft alone.


METHODS
Thirty-two rabbits with 32 critical-size (10 x 5 mm), full-thickness bony defects in rabbit parietal bone were divided into two groups: composite EC-DBM(IM) group-implanted with composite autogenous EC bone and DBM(IM); EC bone group-implanted with EC bone alone. Two rabbits from each group were sacrificed-1, 2, 3, 4, 5, 6, 7, and 14 days after grafting. Neovascularization was assessed by immunohistochemical staining with antihuman angiogenesis-related endothelial cell antibodies (EN 7/44). Quantitative analysis of neovascularization, represented by percentage area of positive immunohistochemical staining, was performed on 320 sections of the experimental groups by a computer-assisted image analyzer.


RESULTS
Positive immunohistochemical staining was first identified on day 2 post grafting for the composite EC-DBM(IM) group in comparison with day 4 in the EC bone graft group. The composite EC-DBM(IM) bone graft group showed earlier and almost 100% more neovascularization when compared with the EC bone graft group.


CONCLUSIONS
DBM(IM) enhances healing and integration of EC bone graft by enhancing vascularization as well as increasing the amount of new blood vessels formed. In clinical cases in which EC autogenous bone is used to graft a large defect such as in cleft palate and craniofacial surgery, DBM(IM) should allow better integration and healing of the EC bone graft to the host bone.
Faculty of Dentistry, University of Hong Kong.
K M
KM
Chow
A B
AB
Rabie
D000818:Animals; D001857:Bone Matrix; D018786:Bone Substitutes / Q000627:therapeutic use*; D016025:Bone Transplantation / Q000379:methods / Q000473:pathology* / Q000502:physiology; D004396:Coloring Agents; D004730:Endothelium, Vascular / Q000254:growth & development*; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D010294:Parietal Bone / Q000601:surgery; D011817:Rabbits; D013997:Time Factors; D014182:Transplantation, Autologous; D014945:Wound Healing
D018786:Bone Substitutes; D004396:Coloring Agents