Fibrous tumors in children - a morphologic and interphase cytogenetic analysis of problematic cases.
We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromosomes 8, 11 and 17. Trisomy 8 and 11 were detected in a high percentage of nuclei in cases of congenital/infantile fibrosarcomas (ranging from 45 to 80%), and in a low grade fibrosarcoma in an older child (23%). Only gains of chromosome 17 were detected in a case of infantile fibromatosis (22%). In this study we have found that given the unconventional histopathologic features, the detection of more than one non-random chromosomal gains by FISH, may aid in further defining fibrous tumors in children, and may be useful as an ancillary diagnostic test in the future.
Department of Pathology, Children's Memorial Hospital, Chicago, IL 60614, USA.
E M
EM
Flores-Stadler
P M
PM
Chou
N
N
Barquin
M
M
Thangavelu
F
F
Gonzalez-Crussi
D000293:Adolescent; D000782:Aneuploidy; D002675:Child, Preschool; D002880:Chromosomes, Human, Pair 11; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D005260:Female; D005350:Fibroma / Q000235:genetics / Q000473:pathology*; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology*; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D007231:Infant, Newborn; D007399:Interphase; D008297:Male; D018224:Myofibromatosis / Q000235:genetics / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000473:pathology*; D014314:Trisomy