The nine C-terminal amino acids of the major capsid protein of the human papillomavirus type 16 are essential for DNA binding and gene transfer capacity.
Four C-terminal deletion mutants of the human papillomavirus 16 L1 protein were expressed in the baculovirus expression system. They consist of the deletion of amino acids 497-505, 477-505, 403-505 and 302-505 (delta C9, delta C31, delta C103 and delta C204 respectively). Only two of the C-terminally deleted proteins, delta C9 and delta C31, retained the ability to form virus-like particles (VLPs) resembling those obtained with the full length L1 protein. Analysis of deleted L1 proteins and corresponding VLPs indicated that the C-terminus was necessary both for DNA binding and DNA packaging. These results were corroborated by the loss of the gene transfer capacities of C-terminal deleted VLPs.
Laboratoire de Virologie Moléculaire, Faculté des Sciences Pharmaceutiques Phillipe Maupas, Tours, France.
A
A
Touzé
D
D
Mahé
S
S
El Mehdaoui
C
C
Dupuy
A L
AL
Combita-Rojas
L
L
Bousarghin
P Y
PY
Sizaret
P
P
Coursaget
D000595:Amino Acid Sequence; D036022:Capsid Proteins; D004247:DNA / Q000378:metabolism*; D017353:Gene Deletion; D018014:Gene Transfer Techniques; D006801:Humans; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D027383:Papillomaviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000378:metabolism / Q000648:ultrastructure; D019065:Virus Assembly
D036022:Capsid Proteins; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; C063317:L1 protein, Human papillomavirus type 16; D004247:DNA