Separation of European flat oyster, Ostrea edulis, haemocytes by density gradient centrifugation and SDS-PAGE characterisation of separated haemocyte sub-populations.
A two-step gradient centrifugation with Percoll and Ficoll successively as density medium was developed to separate European flat oyster, Ostrea edulis, haemocytes into three sub-populations representing granulocytes, large hyalinocytes and small hyalinocytes, respectively. After a Percoll gradient centrifugation, granulocytes and agranulocytes were separated and a pure fraction of granulocytes was obtained. The agranulocytes were further separated by centrifugation through a Ficoll gradient, and two haemocyte subpopulations representing large hyalinocytes and small hyalinocytes were obtained. No significant impact on the haemocyte viability was detected after separation with this two-step density gradient centrifugation. The three haemocyte sub-populations showed different protein patterns in SDS-PAGE.
Xue
Q
Q
IFREMER-Laboratoire de Génétique et Pathologie, La Tremblade, France.
Renault
T
T
Cochennec
N
N
Gerard
A
A
D000818:Animals; D002470:Cell Survival; D002499:Centrifugation, Density Gradient / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006434:Hemocytes / Q000166:cytology*; D008970:Molecular Weight; D010125:Ostreidae / Q000166:cytology*; D011506:Proteins / Q000032:analysis
D011506:Proteins