Role of the yiaR and yiaS genes of Escherichia coli in metabolism of endogenously formed L-xylulose.
Genes yiaP and yiaR of the yiaKLMNOPQRS cluster of Escherichia coli are required for the metabolism of the endogenously formed L-xylulose, whereas yiaS is required for this metabolism only in araD mutants. Like AraD, YiaS was shown to have L-ribulose-5-phosphate 4-epimerase activity. Similarity of YiaR to several 3-epimerases suggested that this protein could catalyze the conversion of L-xylulose-5-phosphate into L-ribulose-5-phosphate, thus completing the pathway between L-xylulose and the general metabolism.
Department of Biochemistry, School of Pharmacy, University of Barcelona, 08028 Barcelona, Spain.
E
E
Ibañez
R
R
Gimenez
T
T
Pedraza
L
L
Baldoma
J
J
Aguilar
J
J
Badia
D002238:Carbohydrate Epimerases / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005816:Genetic Complementation Test; D005810:Multigene Family; D016296:Mutagenesis; D009876:Operon; D015183:Restriction Mapping; D014996:Xylulose / Q000096:biosynthesis / Q000378:metabolism*
D014996:Xylulose; D002238:Carbohydrate Epimerases; C022554:L-ribulosephosphate 4-epimerase