[Immuno-epidemiological investigation of recurrent spontaneous abortion].
OBJECTIVE
To probe into immune pathogeny of recurrent spontaneous abortion (RSA).


METHODS
Immunological analysis was made in ABO group antibodies by indirect hemagglutination, anti-zona pellucida antibodies by BA-ELISA, anti-phospholipid antibodies by ELISA, blocking effect (MLR-BE) by blocking test one-way mixed lymphocyte reaction, cytotoxic index (CI) by complement-dependent cytotoxic test, anti-idiotypic antibodies to blocking antibodies by MLR-BE inhibiting test, and effects of blocking antibodies on CD antigens by flow cytometry. In contrast to the healthy fertile women, 129 cases of primary abortion and 16 cases of secondary abortion were retrospectively analyzed for immunoetiological compositions.


RESULTS
Among causes of RSA, lack of blocking antibodies accounted for 31.7%, anti-zona pellucida antibodies for 20.4%, anti-phospholipid antibodies for 12.5%, and ABO group antibodies for 11.5%. Among causes of primary abortion, lack of blocking antibodies accounted for 31.4%, anti-zona pellucida antibodies for 20.4%, anti-phospholipid antibodies for 8.5%, and ABO group antibodies for 8.4%. Among causes of secondary abortion, imbalance of idiotypic-antiidiotypic network of blocking antibody accounted for 39.4%, anti-phospholipid antibodies for 31.4%, and ABO group antibodies for 22.4%.


CONCLUSIONS
According to the above evaluation of immuno-epidemiology of RSA, RSA may be etiologically classified into lack of materno-fetal immuno-recognition, materno-fetal inappropriate immuno-recognition, and disorder of materno-fetal immuno-recognition.
Laboratory of Reproductive Immunology, Shanghai Medical University.
D
D
Li
Y
Y
Zhu
M
M
Wang
D000017:ABO Blood-Group System / Q000276:immunology*; D000026:Abortion, Habitual / Q000453:epidemiology / Q000209:etiology / Q000276:immunology*; D000906:Antibodies / Q000097:blood; D000888:Antibodies, Anti-Idiotypic / Q000097:blood; D017152:Antibodies, Antiphospholipid / Q000097:blood*; D002681:China / Q000453:epidemiology; D005260:Female; D006801:Humans; D011247:Pregnancy; D012189:Retrospective Studies
D000017:ABO Blood-Group System; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D017152:Antibodies, Antiphospholipid