Differences in the concentration of various synovial fluid constituents between the distal interphalangeal joint, the metacarpophalangeal joint and the navicular bursa in normal horses.
As a prerequisite for the identification of navicular disease markers, the concentrations of cartilage oligomeric matrix protein (COMP), total glycosaminoglycans (GAG), hyaluronan, metalloproteinases (MMP) 2 and 9 and total protein were measured in synovial fluid samples obtained from the distal interphalangeal joint (DIP), the metacarpophalangeal joint (MCP) and the navicular bursa of 24 horses. Mean GAG, COMP and total protein levels were significantly higher in the DIP joint and in the navicular bursa compared to the MCP joint. Hyaluronan content was lower. MMP -2 activity was present in all fluids measured and had similar levels in different joints. MMP -9 was present in 42 per cent of MCP joint samples and 58 per cent of DIP joint samples and of navicular bursal samples. In relation to the constituents measured, the composition of navicular bursal fluid was similar to the articular synovial fluids, in particular that obtained from the DIP joint. Correlation between the constituents of DIP joint fluid and navicular bursal fluid obtained from the same legs was statistically significant for all the parameters measured.
Farm Animal and Equine Medicine and Surgery, Royal Veterinary College, University of London, UK.
M
M
Viitanen
J
J
Bird
P
P
Maisi
R
R
Smith
R M
RM
Tulamo
S
S
May
D000818:Animals; D002061:Bursa, Synovial / Q000737:chemistry / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000032:analysis; D018093:Gelatinases / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis; D006724:Hoof and Claw; D006736:Horses / Q000502:physiology*; D006820:Hyaluronic Acid / Q000032:analysis; D007091:Image Processing, Computer-Assisted; D007596:Joints / Q000737:chemistry / Q000502:physiology*; D064235:Matrilin Proteins; D020778:Matrix Metalloproteinase 2 / Q000032:analysis; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011859:Radiography; D012716:Sesamoid Bones / Q000000981:diagnostic imaging / Q000502:physiology; D013053:Spectrophotometry; D013582:Synovial Fluid / Q000737:chemistry*
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D006025:Glycosaminoglycans; D064235:Matrilin Proteins; D011506:Proteins; D006820:Hyaluronic Acid; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9