[Molecular cloning and sequencing of genes encoding MSP2 isolates strains from two of Plasmodium falciparum from Chinese patients with cerebral malaria].
OBJECTIVE
To provide the scientific evidence for designing safe and effective vaccines of human cerebral malaria.


METHODS
Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients' blood in mengla County, Yunnan Province (CMH/YN) and in Yingjiang County, Yunnan Province (CYJ/YN) were used for polymerase chain reaction (PCR) amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind III respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.


RESULTS
Compared with the published findings, FC27, K1, IC1 and CAMP sequences, DNA sequences of MSP2 from two isolated CMH/YN and CYJ/YN of Plasmodium falciparum strains from Chinese patients with cerebral malaria contained identical genes composed of 800 bp, encoding 264 amino acid, which were highly homologous up to 98.8% with that of FC27, K1 strain other than the IC1, CAMP strain.


CONCLUSIONS
It is the first record of DNA sequencing of MSP2 determined from two isolated CMH/YN and CYJ/YN of Plasmodium falciparum strains from Chinese patients with cerebral malaria, MSP2 mutation may be one factor leading to the localized cerebral damage which causes clinical coma of human cerebral malaria.
Department of Parasitology, Second Military Medical University, Shanghai.
Z
Z
Bian
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G
Song
Z
Z
Zheng
D000595:Amino Acid Sequence; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D016779:Malaria, Cerebral / Q000469:parasitology*; D016778:Malaria, Falciparum / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium