[Two-center evaluation of eight kits for antithyroid peroxidase autoantibodies determinations].
We compared eight antithyroid peroxidase antibody assay kits in two centres, by use of panel sera from 269 patients: controls (n = 100), patients with autoimmune thyroid diseases (n = 77 Graves' disease, Hashimoto's thyroiditis), with non autoimmune thyroid diseases (n = 69 nodular goiter, differentiated thyroid carcinoma), and with autoimmune disease without thyroid pathology (n = 23 diabetic subjects, rheumatoid polyarthritis). On the controls sera we observed different distributions of values. The cut-off values of each kit was, in most cases, similar to the value noted in the manufacturer's instructions. In the clinical study, we observed few differences. The majority of assays demonstrated high diagnostic performance. Some false positive results and the non assessment of some sera by competitive immunoassay were observed.
Service de médecine nucléaire, hôpital Salengro, CHRU, 59037 Lille cedex.
M
M
d'Herbomez
R
R
Sapin
F
F
Gasser
J L
JL
Schlienger
J L
JL
Wémeau
D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D005260:Female; D006044:Goiter, Nodular / Q000097:blood / Q000276:immunology; D006111:Graves Disease / Q000097:blood / Q000276:immunology; D006801:Humans; D007453:Iodide Peroxidase / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D013959:Thyroid Diseases / Q000097:blood* / Q000276:immunology; D013964:Thyroid Neoplasms / Q000097:blood / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000097:blood / Q000276:immunology
D001323:Autoantibodies; D011933:Reagent Kits, Diagnostic; C056626:thyroid microsomal antibodies; D007453:Iodide Peroxidase