[Inhibition of human stomach cancer metastasis in vivo by anti-P-selectin monoclonal antibody].
OBJECTIVE
To study the role of cell adhesion molecule P-selectin monoclonal antibody (Mab) in tumor metastasis of an orthotopic implantation metastatic model.


METHODS
SCID mice were implanted orthotopically using SGC-7901 human stomach carcinoma intact tissue. Three days later, animals were given i.v. injections of PBS or P-selectin Mab (100 micrograms/injection) twice weekly for 3 weeks. 42 days after operation, all animals were sacrificed. Tissues from stomach and other organs were obtained for histopathological evaluation.


RESULTS
Ten of the animals (n = 11) treated with PBS developed metastatic tumors in the regional lymph nodes, liver, and lung. In contrast, 2 of the animals (n = 9) treated with P-selectin Mab developed metastatic tumors in the organs examined. The level of P selectin mRNA expression determined by RT-PCR in gastric carcinoma tissue of SCID mice with tumor metastas is was higher than that without metastasis.


CONCLUSIONS
The expression of P-selectin adhesion moleculeis associated with tumor metastasis, and the metastasis can be arrested by the Mab.
Chen
J
J
Shanghai Sixth Hospital.
Zhang
Y
Y
Chu
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D019007:P-Selectin / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*
D000911:Antibodies, Monoclonal; D019007:P-Selectin