Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci.
Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.
Section of Infection Control, Okayama University Hospital, Okayama 700-8558, Japan. kariyama@med.okayama-u.ac.jp
R
R
Kariyama
R
R
Mitsuhata
J W
JW
Chow
D B
DB
Clewell
H
H
Kumon
D003428:Cross Infection / Q000382:microbiology; D016983:Enterococcus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011159:Population Surveillance; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D020713:Vancomycin Resistance / Q000235:genetics*
D012336:RNA, Ribosomal, 16S