A role for TNF in bone resorption of deciduous molars in human beings.
In this study, tumor necrosis-alpha was sampled from the gingival crevice of human deciduous molars; this was compared with values measured from the crevice of those deciduous molars missing a permanent successor, and from the crevice of deciduous ankylosed molars. Tumor necrosis-alpha was harvested from the gingival crevice with magnetic microspheres coated with tumor necrosis-alpha antibodies. The amount of bead-bound tumor necrosis-alpha was quantified with the use of an enzyme-linked immunosorbent assay. One hundred seven sites (from 41 patients) were sampled; for each patient, the normal value was compared with either the molars missing a permanent successor or ankylosed value. The tumor necrosis-alpha levels were 1.6 times higher from the crevice of ankylosed deciduous molars when compared with normal deciduous molars and 2.6 times higher from the crevice of sites with a molar missing a permanent successor. The mean and standard error mean distribution of tumor necrosis-alpha expressed as picograms was: normal molars 91 pg (standard error mean +/- 20), ankylosed molars 150 pg (standard error mean +/- 31), and missing permanent successor 236 pg (standard error mean +/- 67). Analysis of variance showed the difference among the 3 means was close to attaining significant difference (F [2.104] = 2.7905, P =.066). Multiple comparison procedures indicated that the mean for molars missing a permanent successor and the normal groups were significantly different, P =.05. The results of this study suggest tumor necrosis-alpha values are elevated in the gingival crevice of deciduous molars with ankylosis and where the permanent tooth bud is congenitally missing.
Ureles
S D
SD
School of Dental Medicine, University of Connecticut Health Center, USA. Sureles@aol.com
Chrzan
J M
JM
Norton
L A
LA
Rossomando
E F
EF
D000539:Alveolar Process / Q000378:metabolism*; D000848:Anodontia / Q000378:metabolism; D002648:Child; D003795:Dental Sac / Q000002:abnormalities / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005881:Gingiva / Q000737:chemistry; D006801:Humans; D008863:Microspheres; D008963:Molar / Q000378:metabolism; D020254:Tooth Ankylosis / Q000378:metabolism*; D014078:Tooth Eruption / Q000502:physiology*; D020390:Tooth Socket / Q000737:chemistry; D014094:Tooth, Deciduous / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism / Q000502:physiology*
D014409:Tumor Necrosis Factor-alpha