Molecular cloning and characterization of human FGF-20 on chromosome 8p21.3-p22.
The fibroblast growth factors (FGFs) play important roles in morphogenesis, angiogenesis, tissue remodeling, and carcinogenesis. Human FGF-20 has been cloned and characterized in this study. FGF-20 encodes a 211-amino-acid polypeptide with the FGF-core domain. A strong hydrophobic region was found in the FGF-core domain of FGF-20; however, no typical N-terminal signal sequence was found in FGF-20, just as in FGF-9 and FGF-16. Total amino acid identities are as follows: FGF-20 vs FGF-9, 71.6%; FGF-20 vs FGF-16, 66.2%; FGF-9 vs FGF-16, 72.4%. Phylogenic analysis indicated that FGF-20, FGF-9, and FGF-16 constitute a subfamily among the FGF family. FGF-20 mRNA of 2.4 kb in size was detected in colon cancer cell line SW480 by Northern blot analysis. Lower levels of FGF-20 mRNA were detected in human fetal tissues and primary cancers by cDNA-PCR. The nucleotide sequence of FGF-20 cDNA is split into three parts in the human genome sequence of the chromosome 8p21.3-p22 region (Accession No. AB020858). These results indicate that the FGF-20 gene, located on human chromosome 8p21.3-p22, consists of three exons. Compared with the nucleotide sequence of FGF-20 cDNA determined in this study, one nucleotide deletion and one nucleotide substitution in the putative coding region were identified in human genome sequence AB020858.
Kirikoshi
H
H
Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Tsukiji 5-chome, Chuo-ku, Tokyo, 104-0045, Japan.
Sagara
N
N
Saitoh
T
T
Tanaka
K
K
Sekihara
H
H
Shiokawa
K
K
Katoh
M
M
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005346:Fibroblast Growth Factors / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology; D014981:Xenopus; D029867:Xenopus Proteins
D018076:DNA, Complementary; D012333:RNA, Messenger; D029867:Xenopus Proteins; C120962:fibroblast growth factor 20, Xenopus; D005346:Fibroblast Growth Factors