Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae.
Two fragments that could function as replicational cue elements were isolated from a genomic DNA digest of Aspergillus oryzae on the basis of abnormal behavior in polyacrylamide gel electrophoresis. The vector used in this study contained a scaffold-associated region of the Drosophila melanogaster ftz gene to provide nuclear retention. Neither fragment contained a yeast ARS consensus sequence or an eukaryotic topoisomerase II binding sequence. One of the fragments showed sequence homology with the mitochondrial replication origin of Candida utilis and a portion of mitochondrial DNA of Aspergillus nidulans. This plasmid carrying the cue fragment could also replicate in HeLa and NIH3T3 cells.
Laboratory of Silkworm Sciences, Faculty of Agriculture, Kyusyu University, Fukuoka, Japan. kusakabe@agr.kyushu-u.ac.jp
T
T
Kusakabe
Y
Y
Sugimoto
Y
Y
Hirota
S
S
Toné
Y
Y
Kawaguchi
K
K
Koga
T
T
Ohyama
D000818:Animals; D001236:Aspergillus oryzae / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D022004:Chromosome Structures / Q000235:genetics; D004261:DNA Replication / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008322:Mammals / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D004271:DNA, Fungal