Simian virus 40 induces multiple S phases with the majority of viral DNA replication in the G2 and second S phase in CV-1 cells.
The infection of permissive monkey kidney cells (CV-1) with simian virus 40 induces G1 growth-arrested cells into the cell cycle. After completion of the first S phase and movement into G2, mitosis was blocked and the cells entered another DNA synthesis cycle (second S phase). Growth-arrested CV-1 cells replicated significant amounts of viral DNA in the G2 phase with the majority of synthesis occurring during the second S phase. When mimosine-blocked (G1/S) infected cells were released into the cell cycle, a major portion of the viral DNA was detected in G2 with the largest accumulation in the second S phase. The total DNA produced per infected cell was 10-12C with approximately 0.5-2C of viral DNA replicated per cell. Therefore the majority of the DNA per cell was cellular, 4C from the first S phase and approximately 4-6C from the second cellular synthesis phase.
Department of Microbiology, Immunology, and Molecular Genetics, Center for Immunology and Microbial Diseases, Albany Medical College, New York 12208, USA. lehmanj@mail.amc.edu
J M
JM
Lehman
J
J
Laffin
T D
TD
Friedrich
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D018385:Centrosome / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis; D016195:G2 Phase; D008898:Mimosine / Q000494:pharmacology; D016196:S Phase; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
D004279:DNA, Viral; D008898:Mimosine