Determination of divalent carboxylic acids in traditional Chinese herbal and patent medicines using HPLC with double cell quartz crystal detector.
A rapid, simple and sensitive high performance liquid chromatography (HPLC) method combined with a novel double cell quartz crystal (DCQC) detector is described for the determination of divalent carboxylic acids in traditional Chinese herbal and patent medicines. The chromatographic system involves the use of a muBondapak-NH2 column with 20 mM KH2PO4 (pH 3.4, adjusted by H3PO4)/acetonitrile (95:5%) as a mobile phase. The DCQC detector has a low temperature coefficient and high conductance sensitivity independent of the background from 7.2 to 2500 microS. Analysis of sample is completed without the use of an ion suppression device.
Chen
P
P
New Materials Research Institute, Hunan University, Changsha, People's Republic of China. chen-po@163.net
Zhang
Y
Y
Wei
W
W
Yao
S Z
SZ
D002138:Calibration; D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D011791:Quartz; D013057:Spectrum Analysis / Q000379:methods*
D002264:Carboxylic Acids; D004365:Drugs, Chinese Herbal; D004364:Pharmaceutical Preparations; D011791:Quartz