[8-chloro-adenosine induced apoptosis in various human tumor cell lines].
OBJECTIVE
To study the effect of 8-Chloro-adenosine (8CA) on tumor cell growth and apoptosis.


METHODS
Tumor and normal cell lines were treated with 8CA at different concentrations and cell growth was analysed by MTT assay. Cell apoptosis was evaluated by light microscopy, flow cytometry and chromosome DNA fragmentation.


RESULTS
Treatment with 8CA at 10 mumol/L remarkably inhibited tumor cell growth. HL-60 promyelocytic leukemia cell line was more sensitive to 8CA than MGc-803 gastric mucoid adenocarcinoma cell line. NIH3T3 cells were relatively resistant to 8CA. The IC50 of HL-60 and MGc-803 cells was 1.2 mumol/L and 1.8 mumol/L, respectively, while that of NIH3T3 cells was 12 mumol/L. Further study showed that 8CA induced HL-60 and MGc-803 cell death characterized by apoptotic phenotypes, DNA content changes and chromosome DNA fragmentation.


CONCLUSIONS
8CA is a potential agent for tumor chemotherapy.
Institutes of Basic Medical Sciences and Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
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Zhang
D
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Zheng
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Liu
D015762:2-Chloroadenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D016475:3T3 Cells / Q000187:drug effects; D002288:Adenocarcinoma, Mucinous / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D051379:Mice; D013274:Stomach Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000970:Antineoplastic Agents; D015762:2-Chloroadenosine; C075440:8-chloroadenosine