The Mycobacterium bovis homologous protein of the Mycobacterium tuberculosis serine/threonine protein kinase Mbk (PknD) is truncated.
We previously identified a 70-kDa serine/threonine protein kinase (MbK or PknD) from Mycobacterium tuberculosis Erdman containing a transmembrane domain and bearing a 270-amino acid N-terminal kinase domain. With the use of a polyclonal serum, Mbk has now been identified by Western blotting in protein extracts from M. tuberculosis and confirmed to be localised in the envelope. An identical mbk gene has been found by sequencing different M. tuberculosis and M. africanum strains. Surprisingly, in two virulent M. bovis strains and four different strains of M. bovis BCG, an additional adenine after position 829 of the open reading frame was found that produces a frame shift resulting in a predicted truncated, presumably free cytoplasmic protein, encoding only the N-terminal 30-kDa Mbk kinase domain. This sequence polymorphism has been confirmed by Western blot analysis of M. bovis BCG protein extracts.
Department of Virology, Institut Pasteur de Bruxelles, 642 rue Engeland, 1180, Brussels, Belgium.
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Content
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular; D007106:Immune Sera; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009163:Mycobacterium bovis / Q000201:enzymology / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D013912:Threonine; D012694:Serine; D011494:Protein Kinases; C112455:PknD protein, Nostoc sp. PCC 7120