The effects of sarin-like and soman-like organophosphorus agents on MAPK and JNK in rat brains.
One sarin-like and one soman-like organophosphorus agent [bis(isopropyl methyl)phosphonate, BIMP and bis(pinacolyl methyl)phosphonate, BPMP] were injected intravenously (iv) in rats. An increase in the tyrosine phosphorylation of several proteins in the cytosol fraction of the brain was observed. Activation of c-Jun N-terminal kinase (JNK) and slight activation of mitogen-activated protein kinase (MAPK) in the cytosol were also observed. The activation of these enzymes may be related to the high toxicity of these nerve agents.
Department of Forensic Medicine, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, 113-0033, Tokyo, Japan.
H
H
Niijima
M
M
Nagao
M
M
Nakajima
T
T
Takatori
M
M
Iwasa
Y
Y
Maeno
H
H
Koyama
I
I
Isobe
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002619:Chemical Warfare Agents / Q000009:adverse effects*; D002800:Cholinesterase Inhibitors / Q000009:adverse effects*; D004396:Coloring Agents; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004164:Diphosphonates / Q000009:adverse effects*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D048031:JNK Mitogen-Activated Protein Kinases; D008163:Luminescent Measurements; D048369:MAP Kinase Kinase 1; D008297:Male; D020929:Mitogen-Activated Protein Kinase Kinases / Q000187:drug effects*; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D017346:Protein Serine-Threonine Kinases / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012524:Sarin / Q000009:adverse effects*; D012999:Soman / Q000009:adverse effects* / Q000031:analogs & derivatives*; D013912:Threonine / Q000187:drug effects / Q000378:metabolism; D014443:Tyrosine / Q000187:drug effects / Q000378:metabolism
D002619:Chemical Warfare Agents; D002800:Cholinesterase Inhibitors; D004396:Coloring Agents; D004164:Diphosphonates; C109992:bis(isopropyl methyl)phosphonate; D013912:Threonine; D014443:Tyrosine; C046573:pinacolyl methylphosphonic acid; D012999:Soman; D012524:Sarin; D017346:Protein Serine-Threonine Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048369:MAP Kinase Kinase 1; D020929:Mitogen-Activated Protein Kinase Kinases