Effects of chemical modification of carboxyl groups in the hemolytic lectin CEL-III on its hemolytic and carbohydrate-binding activities.
Effects of chemical modification of carboxyl groups in the hemolytic lectin CEL-III on its activities were investigated. When carboxyl groups were modified with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and glycine methyl ester, hemolytic activity of CEL-III decreased as the EDC concentration increased, accompanied by reduction of oligomerization ability and hemagglutinating activity. However, binding ability of CEL-III for immobilized lactose was retained fairly well after modification, suggesting that one of two carbohydrate-binding sites might be responsible for such inactivation of CEL-III.
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University, Japan.
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H
Kuwahara
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T
Funada
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T
Hatakeyama
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H
Aoyagi
D000818:Animals; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D004910:Erythrocyte Membrane / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits
C402644:CEL-III lectin, Cucumaria echinata; D037102:Lectins