Cholestan-3beta,5alpha,6beta-triol, but not 7-ketocholesterol, suppresses taurocholate-induced mucin secretion by cultured dog gallbladder epithelial cells.
In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects in cultured human pulmonary artery endothelial cells (HPAE). Oxysterols inhibited cell proliferation in a dose-dependent fashion. Oxysterols inhibited cell growth to 50% of control at a higher dose for DGBE cells than for HPAE cells. TriolC was more cytotoxic than 7KC. We also investigated the effect of oxysterols on bile salt-induced mucin secretion by DGBE cells. TriolC suppressed mucin secretion by DGBE cells, whereas 7KC did not. These findings support the hypothesis that biliary oxysterols affect gallbladder mucosal function.
Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA.
T
T
Yoshida
J H
JH
Klinkspoor
R
R
Kuver
S P
SP
Wrenn
E W
EW
Kaler
S P
SP
Lee
D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002777:Cholestanols / Q000494:pharmacology* / Q000633:toxicity; D002857:Chromium / Q000378:metabolism; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005704:Gallbladder / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007653:Ketocholesterols / Q000494:pharmacology* / Q000633:toxicity; D009077:Mucins / Q000378:metabolism*; D011651:Pulmonary Artery; D013656:Taurocholic Acid / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D001647:Bile Acids and Salts; D002777:Cholestanols; D007653:Ketocholesterols; D009077:Mucins; D002857:Chromium; C000474:cholestane-3,5,6-triol; D013656:Taurocholic Acid; C003001:7-ketocholesterol