Effect of the interleukin-1 genotype on monocyte IL-1beta expression in subjects with adult periodontitis.
An association has been reported between polymorphisms in the genes encoding IL-1alpha (-889) and IL-1beta (+3953) (periodontitis susceptibility trait, PST), and an increased severity of periodontitis (18). The IL-1beta polymorphism was reported to correlate with increased IL-1beta expression by monocytes in response to bacterial stimulants. In the present study, we determined if PST positive subjects with periodontitis exhibit elevated production of IL-1beta, compared to PST negative periodontitis patients. Peripheral blood monocytes were obtained from 10 PST+ and 10 PST- age- and disease-balanced subjects with adult forms of periodontitis. Monocytes were cultured with a panel of bacterial stimulants, including Escherichia coli and Porphyromonas gingivalis LPS, and whole formalinized periodontal pathogens P. gingivalis, Bacteroides forsythus and Prevotella intermedia, and health-associated organisms Veillonella parvula and Streptococcus sanguis. Our results demonstrate that monocytes from PST+ and PST- patients showed no significant differences in IL-1beta production in response to any stimulant tested. In addition, the periodontal pathogens P. gingivalis, B. forsythus and P. intermedia failed to stimulate higher IL-1beta responses compared to health-associated species V. parvula and S. sanguis. A marked interindividual variation in production of IL-1beta was seen, with high, low and intermediate responders present in both PST+ and PST- groups. We conclude that genetic loci other than the PST polymorphisms are also important regulators of monocyte IL-1 responses.
Mark
L L
LL
Department of Cytokine Biology, The Forsyth Institute, Boston, MA 02115, USA.
Haffajee
A D
AD
Socransky
S S
SS
Kent
R L
RL
Guerrero
D
D
Kornman
K
K
Newman
M
M
Stashenko
P
P
D000328:Adult; D001439:Bacteroides / Q000472:pathogenicity; D005260:Female; D005786:Gene Expression Regulation; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016130:Immunophenotyping; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D009000:Monocytes / Q000378:metabolism; D010518:Periodontitis / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D011110:Polymorphism, Genetic; D016966:Porphyromonas gingivalis / Q000472:pathogenicity; D018713:Prevotella intermedia / Q000502:physiology; D018709:Statistics, Nonparametric
D007375:Interleukin-1