Digestion of so-called resistant starch sources in the human small intestine.
BACKGROUND
Resistant starch sources, which are only partially digested in the small intestine, can be used to increase colonic availability of short-chain fatty acids.


OBJECTIVE
To study the characteristics of the fermentation of resistant starch, the digestion of resistant starch in the small intestine has to be quantified. We compared the metabolic fates of highly digestible cornstarch (DCS), Hylon VII (type 2 resistant starch), and Novelose 330 (type 3 resistant starch), which are of corn origin and, therefore, naturally enriched in (13)C.


METHODS
After administration of 40 g starch or glucose to 7 healthy volunteers, glucose and exogenous glucose concentrations in serum and (13)CO(2) excretion in breath were analyzed for 6 h. (13)C abundance in carbon dioxide was analyzed by isotope ratio mass spectrometry (IRMS) and (13)C abundance in glucose by gas chromatography-combustion IRMS.


RESULTS
By comparing the area under the curve (2 h) of exogenous glucose concentration in serum ((13)C glycemic index) after intake of starch or glucose, (13)C glycemic indexes for DCS, Hylon VII, and Novelose 330 were calculated to be 82 +/- 23%, 44 +/- 16%, and 43 +/- 15%, respectively. Comparison of 6-h cumulative percentage dose recovery in breath showed that 119 +/- 28% of DCS, 55 +/- 23% of Hylon VII, and 50 +/- 26% of Novelose 330 was digested in the small intestine.


CONCLUSIONS
The exogenous glucose response in serum and the (13)CO(2) excretion in breath can be used to estimate small intestinal digestion of resistant starch, which amounts to approximately 50%.
Department of Pediatrics, Laboratory of Nutrition and Metabolism, University Hospital and University of Groningen, Netherlands. r,j,vonk@med.rug.nl
R J
RJ
Vonk
R E
RE
Hagedoorn
R
R
de Graaff
H
H
Elzinga
S
S
Tabak
Y X
YX
Yang
F
F
Stellaard
D000328:Adult; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism; D001944:Breath Tests; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes; D004063:Digestion; D005260:Female; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism / Q000502:physiology*; D008297:Male; D012016:Reference Values; D013213:Starch / Q000493:pharmacokinetics*
D001786:Blood Glucose; D002247:Carbon Isotopes; D002245:Carbon Dioxide; D013213:Starch