Absence of mutations in the NBS1 gene in B-cell malignant lymphoma patients.
BACKGROUND
Nijmegen breakage syndrome (NBS), also known as ataxia-telangiectasia (AT) variant, is an autosomal recessive disorder characterized by microcephaly, growth retardation, severe combined immunodeficiency and a high incidence of lymphoid carcinoma, the majority of which are B-cell lymphomas. To determine whether the NBS1 gene is a tumor suppressor gene in B-cell lymphoma, we screened B-cell malignant lymphoma (ML) for any evidence of NBS1 mutation.


METHODS
Sequence analysis of the NBS1 gene was performed from PCR products amplified from the DNA of 12 extracranial ML or RT-PCR products amplified from cDNA of 8 primary central nervous system lymphoma.


RESULTS
Direct sequence analysis revealed that no NBS1 mutations were present in any of these patients.


CONCLUSIONS
The present results suggested that the contribution of NBS1 mutations to B-cell ML was minimal, despite the fact that the NBS1 gene was causative factor in these cases.
Department of Radiation Biology, Hiroshima University, Japan. hns@mcai.med.hiroshima-u.ac.jp
S
S
Hama
S
S
Matsuura
H
H
Tauchi
J
J
Sawada
C
C
Kato
F
F
Yamasaki
H
H
Yoshioka
K
K
Sugiyama
K
K
Arita
K
K
Kurisu
N
N
Kamada
Y
Y
Heike
K
K
Komatsu
D000293:Adolescent; D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D005808:Genes, Recessive; D016147:Genes, Tumor Suppressor; D006801:Humans; D007223:Infant; D016393:Lymphoma, B-Cell / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011836:Radiation Tolerance / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003062:Codon; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009687:Nuclear Proteins