In vitro studies on the selective cytotoxic effect of crocetin and quercetin.
Crocetin (5-20 microg/ml), quercetin (10-40 microg/ml), and cisplatin (60-180 microg/ml) used as a positive control drug, were tested against human rhabdomyosarcoma (RD) cells and African green monkey kidney (Vero) cells. The cell viability, morphological changes, and lactate dehydrogenase activity were assessed. RD cell growth was found to be inhibited dose dependently by the three tested compounds. Morphological observation by phase contrast microscopy revealed that both crocetin and quercetin caused intense damage only on the malignant (RD) cells, whereas mild toxic effect was seen with cisplatin also on normal (Vero) cells.
Department of Medical Biochemistry, Dr.A.L.M. PG Institute of Basic Medical Sciences, Taramani Campus, University of Madras, Chennai-600 113, India.
R
R
Jagadeeswaran
C
C
Thirunavukkarasu
P
P
Gunasekaran
N
N
Ramamurty
D
D
Sakthisekaran
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002338:Carotenoids / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D002945:Cisplatin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D012208:Rhabdomyosarcoma / Q000188:drug therapy*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014801:Vitamin A / Q000031:analogs & derivatives
D016588:Anticarcinogenic Agents; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D002945:Cisplatin