[Applications of lecozyme with necrolytic purpose for subsequent keratoplasty in the treatment of eye burns: an experimental study].
Necrolytic properties of lecozyme diluted with urea were studied on a model of isolated alkaline burn of the cornea with 10% NaOH applied for 10 sec. Experimental studies were carried out on both eyes of 62 rabbits (124 eyes). Lecozyme applications in a dose of 17.5 FIP dissolved in urea in concentrations of 3.5 to 40% brought about a complete necrolytic effect in all cases. The results of keratoplasty depended on the concentration of urea. High concentrations (10-40%) caused severe complications in the eye (endophthalmitis). Application of 5-10% urea solutions caused no complications, but the graft healing was opaque in all cases. Satisfactory results were attained with 3.5 and 4% urea. Postoperative course and outcomes of keratoplasty after pretreatment with lecozyme were better than in the control animals subjected to surgical necrectomy. We explain better results of surgical treatment of induced burns by a higher quality of necrectomy, which can be attained by the enzyme. No melting or perforation of the cornea due to the enzyme was observed in any case. Good results of experiments necessitate further experimental and clinical studies.
Chalanova
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D000818:Animals; D002057:Burns, Chemical / Q000473:pathology / Q000628:therapy*; D003315:Cornea / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D010450:Endopeptidases / Q000627:therapeutic use*; D005126:Eye Burns / Q000139:chemically induced* / Q000473:pathology / Q000628:therapy; D015948:Keratoplasty, Penetrating; D009336:Necrosis; D009883:Ophthalmic Solutions / Q000008:administration & dosage; D011300:Preoperative Care; D011817:Rabbits; D012972:Sodium Hydroxide / Q000633:toxicity; D014508:Urea / Q000008:administration & dosage
D009883:Ophthalmic Solutions; D012972:Sodium Hydroxide; D014508:Urea; D010450:Endopeptidases