Cytokines production of U5A2-13-positive T cells by stimulation with glycolipid alpha-galactosylceramide.
We have previously established and reported a novel monoclonal antibody (mAb), U5A2-13, which recognizes a phenotypically similar population of natural killer (NK)-like T cells. Using U5A2-13 mAb, we now describe the functional properties of U5A2-13(+) T cells in both NK1.1-positive or -negative mouse strains. Similar to NK1.1(+) T cells, hepatic U5A2-13(+) T cells of C57BL/6 (NK1.1(+) strain) mice, but not U5A2-13(-) T cells, could be induced to produce large amounts of IL-4 and IFN-gamma by stimulation with glycolipid alpha-galactosylceramide (alpha-GalCer) present on dendritic cells (DC) in a dose-dependent manner. The abundant production of these cytokines from U5A2-13(+) T cells of BALB/c (NK1.1(-) strain) mice is similar to that noted in C57BL/6 mice. Cytokine production by cultures stimulated with DC of beta2-microglobulin-deficient mice was significantly less than that of cultures stimulated with DC of intact mice. Overall, U5A2-13(+) T cells recognize alpha-GalCer presented by CD1d, indicating that U5A2-13(+) T cells can be used to analyze NK-like T cell function in various strains of mice.
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
M
M
Azuma
K
K
Kato
Y
Y
Ikarashi
R
R
Asada-Mikami
H
H
Maruoka
Y
Y
Takaue
A
A
Saito
H
H
Wakasugi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D018949:Antigens, CD1 / Q000502:physiology; D055610:Antigens, CD1d; D000950:Antigens, Ly; D000954:Antigens, Surface; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005699:Galactosylceramides / Q000494:pharmacology*; D015847:Interleukin-4 / Q000502:physiology; D007694:Killer Cells, Natural / Q000378:metabolism*; D037181:Lectins, C-Type; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000941:Antigens; D018949:Antigens, CD1; D055610:Antigens, CD1d; D000950:Antigens, Ly; D000954:Antigens, Surface; D016207:Cytokines; D005699:Galactosylceramides; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D015847:Interleukin-4