Identity of mouse IA-2 and PTP35 genes of the tyrosine phosphatase family, and their expression in neuroendocrine tissues.
Recently, IA-2, one of the major diabetic autoantigens, and PTP35 cDNA were independently isolated by subtraction cloning using insulinoma cells and a polymerase chain reaction (PCR)-based search for conserved sequences using NIH3T3 fibroblast cell line, respectively. By Southern blot analysis and nucleotide sequence determination of reverse transcription PCR products, we showed that IA-2 and PTP35 are identical and exist as a single gene in a mouse genome. The expression of IA-2/PTP35 messages was detected by northern blot analysis in MIN6N8 cells, an insulinoma cell line derived from non-obese diabetic mice, but its expression level was not affected by the ambient glucose level, phorbol-12-myristate 13-acetate or tumour necrosis factor-alpha. We also generated polyclonal antibodies to murine IA-2/PTP35 by immunization with recombinant proteins. Subsequent immunohistochemical analysis using these polyclonal antibodies disclosed that IA-2/PTP35 is strongly expressed in mouse neuroendocrine tissues such as pancreatic islets and the hypothalamus-pituitary gland. These results suggest that IA-2/PTP35 functions primarily in neuroendocrine tissues.
Suk
K
K
Clinical Research Center, Samsung Biomedical Research Institute, Seoul, South Korea.
Hwang
D Y
DY
Kim
S
S
Kim
Y H
YH
Kim
K A
KA
Seo
Y S
YS
Suh
Y
Y
Lee
S B
SB
Namkung
Y
Y
Shin
H S
HS
Lee
M S
MS
D016475:3T3 Cells; D000818:Animals; D001323:Autoantibodies / Q000235:genetics*; D001324:Autoantigens / Q000235:genetics; D001483:Base Sequence; D007150:Immunohistochemistry; D007340:Insulinoma / Q000235:genetics; D007515:Islets of Langerhans / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D009490:Neurosecretory Systems / Q000201:enzymology / Q000276:immunology*; D010190:Pancreatic Neoplasms / Q000235:genetics; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D001323:Autoantibodies; D001324:Autoantigens; C096322:ICA512 autoantibody; D011994:Recombinant Proteins; D017027:Protein Tyrosine Phosphatases