Differences in bone turnover and skeletal response to thyroid hormone treatment between estrogen-depleted and repleted rats.
This study was undertaken to compare the effect of supraphysiological doses of thyroxine (T4) on bone metabolism in SHAM and OVX young adult rats. Female Sprague Dawley rats (220 +/- 2 g, approx. 5 months of age) were divided into four groups of eight animals each. The animals were intraperitoneally injected 6 days per week with vehicle (Vh): 0.001 N NaOH/0.9% NaCl (SHAM+Vh and OVX+Vh) or 250 microg of thyroxine/kg/day (SHAM+T4 and OVX+T4) during a 5-week period. Serum T4 and osteocalcin (BGP), urinary pyridinolines (Pyr), and creatinine (creat) were determined. At the beginning and at end of the experiment, skeletal bone mineral content (BMC), bone mineral density (BMD), and area (A) of the total skeleton, femur, spine, and whole tibia, as well as proximal, middle, and distal areas of the tibia were assessed by dual X-ray absorptiometry (DXA) in an ultra-high-resolution mode. T4 treatment of the SHAM rats did not induce significant changes in BGP level or Pyr/creat excretion compared with the SHAM+Vh control group. However, these two biochemical bone markers significantly increased due to T4 treatment in OVX rats compared with both OVX+Vh and SHAM+T4 groups (P < 0.05 and P < 0.001, respectively). The OVX+T4 group had a significantly lower DeltaBMD than SHAM+T4 rats in all studied regions (P < 0.05) except for the middle tibia region. OVX+T4 groups presented a significantly lower DeltaBMC and DeltaA compared with SHAM+T4 animals (P < 0.001). OVX+T4 rats significantly impaired the DeltaBMD in the femur (P < 0.01), spine (P < 0.05), whole (P < 0.05) and middle (P < 0.05) tibia whereas T4 treatment of SHAM rats only affected, significantly, the whole (P < 0.05) and the proximal tibia region (P < 0.01). T4 treatment affects bone growth in young adult rats. The effect is significantly greater in the estrogen-depleted than in the estrogen-repleted state. The bone site most adversely affected by T4 treatment depends on the estrogen status. The proximal tibia (principally trabecular bone) was the most affected area in estrogen-repleted rats. Conversely, in OVX rats, the middle tibia (principally cortical bone) presented the greatest decrease in bone density.
Sección Osteopatías Médicas, Hospital de Clínicas, Cordoba 2351-piso 8-José de San Martin, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
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Zeni
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Gomez-Acotto
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Di Gregorio
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Mautalen
D015502:Absorptiometry, Photon; D000596:Amino Acids / Q000652:urine; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects*; D003404:Creatinine / Q000652:urine; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging; D015675:Osteocalcin / Q000097:blood; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013131:Spine / Q000000981:diagnostic imaging; D013974:Thyroxine / Q000097:blood / Q000494:pharmacology*; D013977:Tibia / Q000000981:diagnostic imaging
D000596:Amino Acids; D015415:Biomarkers; D015675:Osteocalcin; C015484:pyridinoline; D003404:Creatinine; D013974:Thyroxine