Elicitation of suspension-cultured tomato cells triggers the formation of phosphatidic acid and diacylglycerol pyrophosphate.
Phosphatidic acid (PA) and its phosphorylated derivative diacylglycerol pyrophosphate (DGPP) are lipid molecules that have been implicated in plant cell signaling. In this study we report the rapid but transient accumulation of PA and DGPP in suspension-cultured tomato (Lycopersicon esculentum) cells treated with the general elicitors, N,N',N",N"'-tetraacetylchitotetraose, xylanase, and the flagellin-derived peptide flg22. To determine whether PA originated from the activation of phospholipase D or from the phosphorylation of diacylglycerol (DAG) by DAG kinase, a strategy involving differential radiolabeling with [(32)P]orthophosphate was used. DAG kinase was found to be the dominant producer of PA that was subsequently metabolized to DGPP. A minor but significant role for phospholipase D could only be detected when xylanase was used as elicitor. Since PA formation was correlated with the high turnover of polyphosphoinositides, we hypothesize that elicitor treatment activates phospholipase C to produce DAG, which in turn acts as substrate for DAG kinase. The potential roles of PA and DGPP in plant defense signaling are discussed.
Swammerdam Institute for Life Sciences, Department of Plant Physiology, University of Amsterdam, Kruislaan 318, NL-1098 SM Amsterdam, Netherlands.
A H
AH
van der Luit
T
T
Piatti
A
A
van Doorn
A
A
Musgrave
G
G
Felix
T
T
Boller
T
T
Munnik
D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D011756:Diphosphates / Q000378:metabolism*; D005408:Flagellin / Q000494:pharmacology; D005990:Glycerol / Q000031:analogs & derivatives* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D009844:Oligosaccharides / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010712:Phosphatidic Acids / Q000096:biosynthesis / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000494:pharmacology
D011756:Diphosphates; D009844:Oligosaccharides; D010446:Peptide Fragments; D010712:Phosphatidic Acids; D010743:Phospholipids; C408642:diacylglycerol pyrophosphate; D005408:Flagellin; C003387:tetra-N-acetylchitotetraose; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; D005990:Glycerol