Survival in experimental Candida albicans infections depends on inoculum growth conditions as well as animal host.
Evidence is presented that the growth medium used to prepare a Candida albicans challenge inoculum is a significant factor determining the ability of a fungus strain to gain an initial invasive hold immediately after injection into an animal host, and thus determining gross strain lethality. Three C. albicans strains, one known to be attenuated in virulence, were grown in two broth media and injected intravenously at different doses into female NMRI mice and male albino guinea pigs. For each fungus strain and challenge dose, survival was longer from inocula grown in a diluted, buffered peptone-based broth than from inocula grown in Sabouraud glucose broth. When animals were challenged intravenously with yeast doses adjusted to give the same mean survival time regardless of strain or growth medium, the progression of fungus tissue burdens (c. f.u. g(-1)) in kidneys, lungs, liver, spleen and brain samples was broadly similar for all three C. albicans strains but differed between the two animal hosts. The morphological form of C. albicans recovered from infected tissues differed at the level of both the fungus strain and the host tissue. Use of survival-standardized inocula provides a means of distinguishing differences in progression of experimental disseminated Candida infections that are related to the infecting strain from those related to the animal host.
Department of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, University of Aberdeen, Aberdeen AB25 2ZD, UK1.
Frank C
FC
Odds
Department of Bacteriology and Mycology, Janssen Research Foundation,B-2340 Beerse, Belgium2.
Luc
L
Van Nuffel
Department of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, University of Aberdeen, Aberdeen AB25 2ZD, UK1.
Neil A R
NAR
Gow
D000818:Animals; D001921:Brain / Q000382:microbiology; D002176:Candida albicans / Q000166:cytology / Q000254:growth & development* / Q000472:pathogenicity*; D002177:Candidiasis / Q000209:etiology* / Q000382:microbiology; D015169:Colony Count, Microbial; D003470:Culture Media; D005260:Female; D006168:Guinea Pigs; D007668:Kidney / Q000382:microbiology; D008297:Male; D051379:Mice; D010641:Phenotype; D013045:Species Specificity; D014774:Virulence
D003470:Culture Media