The C5a receptor is expressed by human renal proximal tubular epithelial cells.
The C5a receptor is expressed by a variety of cell types. These studies demonstrate by immunohistochemistry that the receptor is present on the surface of proximal and distal tubular epithelial cells from normal kidney. In addition, the receptor was detected on transitional epithelial cells of the ureter and bladder. Primary proximal tubular cultures and a proximal tubular cell line both also expressed the C5a receptor, as demonstrated by immunofluorescence and by FACS analysis. The presence of mRNA encoding the receptor was confirmed by reverse transcriptase-polymerase chain reaction analysis. As opposed to its effect on glomerular mesangial cells, the receptor did not mediate a proliferative response by the proximal tubular cells. C5a also did not enhance the synthesis/secretion of transforming growth factor-beta 1, monocyte chemoattractant protein-1, platelet-derived growth factor-AB or tumour necrosis factor-alpha by cultured proximal tubular cells. Therefore, although the C5a receptor clearly is expressed by proximal tubular cells, clarification of its functional relevance on this cell type awaits further studies.
The Center for Blood Research, Boston, MA, Immune Cell Interaction Unit, Mucosal Immunity Section, Laboratory of Clinical Investigation, NIAID, Bethesda, MD, USA.
R
R
Zahedi
M
M
Braun
R A
RA
Wetsel
B H
BH
Ault
A
A
Khan
T R
TR
Welch
M
M
Frenzke
A E
AE
Davis
D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D002455:Cell Division; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D005920:Glomerular Mesangium / Q000166:cytology; D006133:Growth Substances / Q000032:analysis; D006801:Humans; D007686:Kidney Tubules, Distal / Q000737:chemistry; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D009130:Muscle, Smooth / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020298:U937 Cells / Q000378:metabolism; D014513:Ureter / Q000737:chemistry; D001743:Urinary Bladder / Q000737:chemistry
D015703:Antigens, CD; D016207:Cytokines; D006133:Growth Substances; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement