Are Kupffer cells involved in the metabolic adaptation of the liver to dietary carbohydrates given after fasting?
In rats, a high carbohydrate fat-free (HCFF) diet, given after fasting, induces both hepatic lipogenic and glycogenic enzymes. In the present study, we evaluated the involvement of Kupffer cells in the metabolic events occurring in the liver during the fasting-refeeding transition. Male Wistar rats were fasted for 48 h and received an intravenous injection of either NaCl 0.9% (Gd-) or 10 mg/kg GdCl(3) (Gd+), an inhibitor of Kupffer cells, then fed for 12 h with a HCFF diet. The comparison of colloidal carbon uptake was similar in rats fasted and in rats fasted and then refed a HCFF diet, thus indicating that refeeding does not affect per se Kupffer cell phagocytic activity. The inhibition of Kupffer cells by GdCl(3) did not affect fatty acid synthase (FAS) induction, as shown by the analysis of both FAS mRNA and activity; refeeding a HCFF diet increased the hepatic triglyceride and glycogen content to the same extent in Gd+ and Gd- rats. Our results do not support the involvement of Kupffer cells in the metabolic events occurring in the liver tissue by feeding a HCFF diet after fasting. However, the discussion supports the involvement of Kupffer cells in the modulation of the hepatic lipid metabolism by other nutrients than carbohydrates.
Unité de Pharmacocinétique, Métabolisme, Nutrition et Toxicologie, Département des Sciences Pharmaceutiques, Université Catholique de Louvain, 73 Avenue Mounier, B-1200 Brussels, Belgium.
A M
AM
Neyrinck
L D
LD
De Wispelaere
V P
VP
Vanhulle
H S
HS
Taper
N M
NM
Delzenne
D000222:Adaptation, Physiological; D000818:Animals; D002244:Carbon; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects; D005215:Fasting; D064429:Fatty Acid Synthases / Q000096:biosynthesis / Q000235:genetics; D005682:Gadolinium / Q000494:pharmacology; D007728:Kupffer Cells / Q000187:drug effects / Q000502:physiology*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010587:Phagocytosis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000032:analysis / Q000097:blood
D004040:Dietary Carbohydrates; D012333:RNA, Messenger; D014280:Triglycerides; D002244:Carbon; D005682:Gadolinium; D064429:Fatty Acid Synthases; C038958:gadolinium chloride