Epigallocatechin gallate increase the prostacyclin production of bovine aortic endothelial cells.
We describe the effect of (-) epigallocatechin gallate (EGCg), one of catechins known in tea, on the prostacyclin (PGI) production by bovine aortic endothelial cells. The amounts of 6-keto-PGF(1alpha) and Delta(17)-6-keto-PGF(1alpha), stable metabolites of PGI(2) and PGI(3), released in culture medium were measured using gas chromatography/selected ion monitoring (GC/SIM). The prostacyclin production of endothelial cells was increased by EGCg in a dose- and time-dependent manner. The effect by EGCg was stronger than any other catechins (catechin, epicatechin, epigallocatechin, and epicatechin gallate). When endothelial cells incubated with EGCg and arachidonic acid (AA) or eicosapentaenoic acid (EPA), PGI(2), and PGI(3) production were increased greater than those incubated with AA or EPA alone. Furthermore, gallic acid, that also has a pyrogallol structure, increased PGI(2) production. These observations indicate that catechins increase the prostacyclin production and that the pyrogallol structure is significant to this function.
Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1, Seiryo-machi, Aoba-ku, 980-8574, Sendai, Japan. mizugaki@mail.cc.tohoku.ac.jp
M
M
Mizugaki
F
F
Ishizawa
T
T
Yamazaki
T
T
Hishinuma
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002392:Catechin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis* / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology; D005707:Gallic Acid / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D015394:Molecular Structure; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D013931:Thromboxanes / Q000378:metabolism
D005343:Fibrinolytic Agents; D010975:Platelet Aggregation Inhibitors; D013931:Thromboxanes; D016718:Arachidonic Acid; D005707:Gallic Acid; C035276:eicosapentaenoic acid ethyl ester; D002392:Catechin; D015118:Eicosapentaenoic Acid; C045651:epigallocatechin gallate; D011464:Epoprostenol