Regulation of the biosynthesis of acyl analogs of platelet-activating factor by purinergic agonist in endothlial cells.
We have previously shown that platelet-activating factor (PAF)-dependent transacetylase (TA) contains three catalytic activities, namely PAF: lysophospholipid TA (TAL), PAF: sphingosine TA (TAs) and PAF acetylhydrolase. It serves as a modifier of PAF actions by producing different lipid signal molecules. The TAL activity is involved in the biosynthesis of acyl analogs of PAF (acyl-PAF, 1-acyl-2-acetyl-sn-glycero-3-phosphocholine, acylacetyl-GPC) in agonist-stimulated endothelial cells. In the present investigation, we have studied the mechanism(s) by which the TA activity is regulated in ATP-treated endothelial cells. We have demonstrated that ATP, and thiol-modifying agents with ATP, specifically regulate only the TAL part of the TA activities.
Basic and Applied Research Unit, Oak Ridge Associated Universities, TN 37831-0117, USA.
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Balestrieri
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D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000123:Acetyltransferases / Q000378:metabolism; D000215:Acylation; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000096:biosynthesis; D013439:Sulfhydryl Reagents / Q000494:pharmacology
C069030:1-acyl-2-acetyl-sn-glycero-3-phosphocholine; D010972:Platelet Activating Factor; D013439:Sulfhydryl Reagents; D000255:Adenosine Triphosphate; D000123:Acetyltransferases; C097632:sphingosine CoA-independent transacetylase; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase