Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation.
The filamentous soil bacterium Streptomyces coelicolor undergoes a complex cycle of morphological differentiation involving the formation of an aerial mycelium and the production of pigmented antibiotics. We have developed a procedure for generating insertional mutants of S. coelicolor based on in vitro transposition of a plasmid library of cloned S. coelicolor DNAs. The insertionally mutated library was introduced into S. coelicolor, and transposon insertions were recovered at widely scattered locations around the chromosome. Many of the insertions revealed previously uncharacterized genes, and several caused novel mutant phenotypes, such as altered pigment production, enhanced antibiotic sensitivity, delayed or impaired formation of aerial hyphae, and a block in spore formation. The sporulation mutant harbored an insertion in one of three adjacent genes that are apparently unique to Streptomyces but are each represented by at least 20 paralogs at dispersed locations in the chromosome. Individual members of the three families often are found grouped together in a characteristic arrangement, suggesting that they have a common function.
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
A M
AM
Gehring
J R
JR
Nodwell
S M
SM
Beverley
R
R
Losick
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics*; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D010641:Phenotype; D020161:Physical Chromosome Mapping; D010860:Pigments, Biological / Q000096:biosynthesis; D011995:Recombination, Genetic / Q000235:genetics; D016415:Sequence Alignment; D013171:Spores, Bacterial / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D010860:Pigments, Biological; D009328:Nebramycin; C011666:apramycin