Use of electroporation to accelerate the skin permeability enhancing action of oleic acid.
Rat skin permeability after treatment by electroporation (newly developed frog type electrode, 100V, 10 pulses), oleic acid/propylene glycol (PG) and a combination of both were investigated using Fourier transformed infrared attenuated total reflectance (FT-IR/ATR) analysis. Electroporation immediately disordered the stratum corneum lipid structure up to a certain threshold level. This action lasted throughout the experiment. This may be attributed to the formation of long lifetime of metastable lipid structures, which may allow molecules to pass to the inside of the stratum corneum due to the electroporation-induced fluidized lipid membranes. Electroporation also altered the protein structure of the stratum corneum. When electroporation was combined with 0.05 M oleic acid/PG, uptake of oleic acid and PG into the stratum corneum was remarkably accelerated compared to the application of only 0.05 M oleic acid/PG to the skin. This indicates that electroporation enables oleic acid and PG to penetrate the stratum corneum easily by disrupting the structure of the latter. PG transfer into the dermis from the epidermis was accelerated, not because of the direct action of electroporation on the dermis, but because electroporation induced the rapidly disordering action of oleic acid on the stratum corneum. Lipid-soluble indomethacin permeated the skin more rapidly when the skin was treated with electroporation plus oleic acid/PG than with 0.05 M oleic acid/PG in vitro.
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Kobe, Japan. takeuchi@pharm.kobegakuin.ac.jp
Y
Y
Takeuchi
K
K
Miyawaki
S
S
Kamiyabu
S
S
Fukushima
Y
Y
Yamaoka
S
S
Kishimoto
K
K
Taguchi
H
H
Masai
Y
Y
Kamata
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000493:pharmacokinetics; D018274:Electroporation; D066298:In Vitro Techniques; D007213:Indomethacin / Q000493:pharmacokinetics; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D019301:Oleic Acid / Q000494:pharmacology*; D019946:Propylene Glycol / Q000494:pharmacology; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D012869:Skin Absorption; D012997:Solvents / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008055:Lipids; D012997:Solvents; D019301:Oleic Acid; D019946:Propylene Glycol; D007213:Indomethacin