[Cloning and expression of a gene associated with HL60 cell apoptosis induced by inhibition of polyamine biosynthesis].
OBJECTIVE
To clone the gene associated with apoptosis induced by an inhibitor of polyamine biosynthesis, alpha-difluoromethylomithine (DFMO).


METHODS
The differential substraction screening was used for gene cloning. The gene expression and apoptosis of transfected HL60 cells were detected by Northern blot, morphological assay, FCM and ladder map of DNA electrophoresis, respectively.


RESULTS
An apoptosis-associated gene named dF4 was cloned from HL60 cells treated with polyamine biosynthesis inhibitor. The programmed cell death was demonstrated in the HL60 cells transfected by dF4 gene.


CONCLUSIONS
dF4 gene cloned in this study could be a gene regulating apoptosis of HL60 cells.
Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing.
L
L
Feng
M
M
Fan
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D000518:Eflornithine / Q000494:pharmacology*; D015870:Gene Expression; D015723:Gene Library; D018922:HL-60 Cells / Q000473:pathology; D006801:Humans
D000970:Antineoplastic Agents; D000518:Eflornithine