The dynamics of neuronal death: a time-lapse study in the retina.
Using time-lapse video microscopy, we have followed the process of neuronal death in an intact region of the mammalian nervous system. We show here the fast dynamics of nuclear fragmentation, which is over in <1 hr for neurons undergoing apoptosis in the living rat retina. Nuclear fragmentation is accompanied by a progressive raise of intracellular calcium and followed by erratic movement of the apoptotic cells, documenting their loss of adhesion.
Istituto di Neurofisiologia del Consiglio Nazionale delle Ricerche, 56017 Pisa, Italy.
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Cellerino
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Galli-Resta
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Colombaioni
D000578:Amidines; D000814:Aniline Compounds; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D053938:DNA Fragmentation / Q000502:physiology; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D018715:Microscopy, Video; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000166:cytology* / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology; D013997:Time Factors; D014966:Xanthenes
D000578:Amidines; D000814:Aniline Compounds; D005456:Fluorescent Dyes; D014966:Xanthenes; C059715:Fluo-3; C037945:diamidino yellow; D002118:Calcium