Up-regulation of the TrkB receptor in mice injured by the partial ligation of the sciatic nerve.
Partial nerve injury induced by tying a tight ligature around the sciatic nerve induced a marked hyperalgesia, and this persistent painful state lasted for 14 days in mice. Under these conditions, the nerve injury induced a significant increase in protein level of protein kinase Cgamma isoform in plasma membranes in the spinal cord. We report here for the first time that protein level of TrkB receptor located in plasma membranes was clearly up-regulated in the spinal cord obtained from the nerve-injured mice. These findings suggest that the up-regulation of protein kinase Cgamma associated with activated TrkB receptors following partial sciatic nerve ligation may induce sensitization of synaptic transmission and may in turn cause the persistent pain in mice.
Department of Toxicology, School of Pharmacy, Hoshi University, 2-4-41, Ebara, Shinagawa-ku, 142-8501, Tokyo, Japan.
M
M
Narita
Y
Y
Yajima
T
T
Aoki
S
S
Ozaki
H
H
Mizoguchi
L F
LF
Tseng
T
T
Suzuki
D000818:Animals; D006930:Hyperalgesia / Q000378:metabolism / Q000503:physiopathology; D007527:Isoenzymes / Q000378:metabolism; D008026:Ligation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011493:Protein Kinase C / Q000378:metabolism; D020813:Receptor, trkB / Q000378:metabolism*; D012584:Sciatic Nerve / Q000503:physiopathology* / Q000601:surgery; D013116:Spinal Cord / Q000201:enzymology / Q000378:metabolism; D015854:Up-Regulation
D007527:Isoenzymes; C087857:protein kinase C gamma; D020813:Receptor, trkB; D011493:Protein Kinase C