Application of a single-cell gel electrophoresis (comet) assay to screen the antimutagenic activity in foods.
Three cell lines (HL60, U937 and RAW264.7) were studied for their sensitivity against mutagens by using a single-cell gel electrophoresis (comet) assay. RAW264.7, the most sensitive one, was chosen to screen the antimutagenic activity in swine and bovine offal. Aqueous extracts of the swine stomach (0.2 mg/ml) and heart (10 mg/ml) were found to have antimutagenic activity against MeIQx (+ S9mix)-treated cells.
Miwa
M
M
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, Japan. mimiwa@nfri.affrc.go.jp
Hongo
Y
Y
D000818:Animals; D016587:Antimutagenic Agents / Q000032:analysis*; D002417:Cattle; D002460:Cell Line; D020552:Comet Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D018922:HL-60 Cells; D006801:Humans; D051379:Mice; D009206:Myocardium / Q000737:chemistry; D011810:Quinoxalines / Q000037:antagonists & inhibitors / Q000633:toxicity; D013270:Stomach / Q000737:chemistry; D013552:Swine; D020298:U937 Cells
D016587:Antimutagenic Agents; D011810:Quinoxalines; C045421:3,4,8-trimethylimidazo(4,5-f)quinoxalin-2-amine