A computational fluid dynamic model of antigen-antibody surface adsorption on a piezoelectric immunosensor.
A novel computational fluid dynamic model describing the antigen-antibody binding on an electrode surface is presented. It was assumed that the adsorption rate of the antibody sample is dependent upon the flow field in the vicinity of the electrode. Numerical solution of the steady flow in a two-dimensional triangular cell using the Navier-Stokes equations was carried out for predicting mass adsorption on the surface of the crystal. The relationships between the mass adsorbed over the area surface of the electrode, the kinetics of the binding process, and the flow field were determined. The effect of the inlet conditions (location, velocity magnitude, and direction) on the time constant of the mass adsorption process was investigated. It was found that the time constant was decreased by moving the inlet near the edge of the crystal or increasing the normal to the boundary component of the velocity. These changes may significantly reduce the time needed to conduct the test.
Department of Biomedical Engineering, Faculty of Engineering, Tel-Aviv University, Israel.
D
D
Barak-Shinar
M
M
Rosenfeld
E
E
Zisman
S
S
Abboud
D000327:Adsorption; D000937:Antigen-Antibody Reactions; D001698:Biomedical Engineering; D015374:Biosensing Techniques; D066298:In Vitro Techniques; D008954:Models, Biological