Immunodiagnosis of chronic Chagas' disease using the Tc 46 and Tc 58 antigens.
The polypeptides of 46 and 58 kDa were recognized in different T. cruzi strains (Y, WSL and Colombiana) by serum of all chagasic patients studied. These polypeptides were isolated from T. cruzi Y strain and used in ELISA. The sensitivity and specificity were 97.6% [CI 95%: 86-100%] and 100% [CI 95%: 89.3-100%], respectively when Tc 46 was used. When Tc 58 was used the sensitivity and specificity were 100% [CI 95%: 89.6-100%] and 90.2% [CI 95%: 75.9-96.8%], respectively.
Pereira
V R
VR
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, Recife, PE, Brasil.
Nakazawa
M
M
Furtado
V C
VC
Abath
F G
FG
Gomes
Y M
YM
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D014355:Chagas Disease / Q000175:diagnosis*; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008970:Molecular Weight; D012680:Sensitivity and Specificity; D014349:Trypanosoma cruzi
D000953:Antigens, Protozoan