Specific induction of Z-DNA conformation by a nuclear localization signal peptide of lupin glutaminyl tRNA synthetase.
Recently we have sequenced cDNA of plant glutaminyl-tRNA synthetase (GlnRS) from Lupinus luteus. At the N terminal part the protein contains a lysine rich polypeptide (KPKKKKEK), which is identical to a nuclear localization signal (NLS). In this paper we showed that two synthetic peptides (20 and 8 amino acids long), which were derived from lupin GlnRS containing the NLS sequence interact with DNA, but one of them (8aa long) changing its conformation from the B to the Z form. This observation clearly suggests that the presence of the NLS polypeptide in a leader sequence of GlnRS is required not only for protein transport into nucleus but also for regulation of a gene expression. This is the first report suggesting a role of the NLS signal peptide in structural changes of DNA.
Institute of Bioorganic Chemistry Polish Academy of Sciences, Poznañ.
A
A
Krzyzaniak
M
M
Siatecka
A
A
Szyk
P
P
Mucha
P
P
Rekowski
G
G
Kupryszewski
J
J
Barciszewski
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D002942:Circular Dichroism; D018744:DNA, Plant / Q000737:chemistry* / Q000378:metabolism*; D007887:Fabaceae / Q000201:enzymology; D008969:Molecular Sequence Data; D019913:Nuclear Localization Signals; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism; D010946:Plants, Medicinal
D018744:DNA, Plant; D019913:Nuclear Localization Signals; D010446:Peptide Fragments; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase