Trichomonas vaginalis: analysis of a heat-inducible member of the cytosolic heat-shock-protein 70 multigene family.
A 2253-nucleotide (nt) transcript for a Trichomonas vaginalis heat-shock protein 70, TVCHSP70, has been isolated that encodes for a protein of 659 amino acids with a predicted molecular weight of 71.3 kDa. TVCHSP70 has a short (10-nt) 5' untranslated region (UTR), and the 263-nt 3' UTR is the longest reported for a Trichomonas peptide. Amino-acid sequence analysis and phylogenetic comparison identifies TVCHSP70 as a member of the heat-inducible cytoplasmic HSP70 gene family. Southern-blot data indicate that T. vaginalis contains at least four members of the cytoplasmic HSP70 gene family. Members of the TVCHSP70 family are expressed as 2.3-kb transcripts at low levels during 37 degrees C culture, and their expression is significantly up-regulated at 43 degrees C. Slot-blot analysis of seven T. vaginalis clinical isolates demonstrated a 3- to 44-fold up-regulation of TVCHSP70 under conditions of heat shock (43 degrees C) or oxidative stress (500 microm H2O2) as compared with controls (37 degrees C).
Davis-Hayman
S R
SR
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505, USA.
Shah
P H
PH
Finley
R W
RW
Lushbaugh
W B
WB
Meade
J C
JC
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003600:Cytosol / Q000378:metabolism; D016054:DNA, Protozoan; D017125:Genes, Protozoan; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger; D016053:RNA, Protozoan / Q000032:analysis; D014246:Trichomonas vaginalis / Q000235:genetics*
D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan