The c-Abl tyrosine kinase contributes to the transient activation of MAP kinase in cells plated on fibronectin.
Previous work showed that integrin stimulation triggers activation of the c-Abl tyrosine kinase and its transient localization to focal adhesions. We now report that plating cells on fibronectin triggers association of Grb2 with c-Abl, suggesting possible involvement of c-Abl with integrin activation of the MAP kinase pathway. Expression of a kinase-defective c-Abl specifically inhibited the transient induction of Erk2 activity following cell adhesion. Together with the known ability of activated, oncogenic forms of c-Abl to activate Ras and the MAP kinase pathway, these data suggest that c-Abl contributes to the integrin induction of MAP kinase activity.
Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
M W
MW
Renshaw
J M
JM
Lewis
M A
MA
Schwartz
D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D004789:Enzyme Activation; D005353:Fibronectins / Q000378:metabolism*; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D051380:GRB2 Adaptor Protein; D020935:MAP Kinase Signaling System; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D016315:Proto-Oncogene Proteins c-abl / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D005353:Fibronectins; D051380:GRB2 Adaptor Protein; C495949:Grb2 protein, mouse; D011506:Proteins; D011505:Protein-Tyrosine Kinases; D051417:Focal Adhesion Kinase 1; D051416:Focal Adhesion Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl; C496051:Ptk2 protein, mouse; D019950:Mitogen-Activated Protein Kinase 1; D020928:Mitogen-Activated Protein Kinases