Genomic organization of the leukotriene B(4) receptor locus of human chromosome 14.
The genomic region containing the genes encoding the first leukotriene B(4) receptor, BLTR, as well as the recently cloned second leukotriene B(4)-activated receptor, BLTR2, was mapped by (a) sequence analysis of a human bacterial artificial chromosome (BAC) library containing a 15-kb segment corresponding to chromosome 14q11. 2-12 where the BLTR/BLTR2 genes were previously shown to be located, together with (b) sequence analysis of 83 expressed sequence tags (ESTs) from this region. The BLTR gene includes four different 5' untranslated regions (UTRs) and a mutual acceptor site for the exon containing the intronless open reading frame. The BLTR2 gene is intronless and overlapped by a 5' UTR splice version of BLTR and, on the reverse strand, of the apoptosis-related CIDE-B gene. This indicates a complex posttranscriptional gene regulation. Further adding to the complexity of the region is evidence of a fourth putative and novel gene, most homologous to the rat adenylyl cyclase IV gene.
Division of Molecular Neurobiology, Wallenberg Neuroscience Center, Lund, SE-22362, Sweden.
N E
NE
Nilsson
Y
Y
Tryselius
C
C
Owman
D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D017398:Alternative Splicing / Q000235:genetics; D051017:Apoptosis Regulatory Proteins; D002876:Chromosomes, Bacterial / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D020224:Expressed Sequence Tags; D015698:Genomic Library; D006801:Humans; D020161:Physical Chromosome Mapping; D011506:Proteins / Q000235:genetics; D018102:Receptors, Leukotriene B4 / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D051017:Apoptosis Regulatory Proteins; C112511:CIDEB protein, human; D018076:DNA, Complementary; D011506:Proteins; D018102:Receptors, Leukotriene B4