[Expression of mdr-1 gene in cancer tissue and its association with morphological indexes of esophageal carcinoma].
OBJECTIVE
To detect the levels of mdr-1 gene expression in fresh untreated esophageal carcinomas, and to correlate these levels to current prognostic indicators of morphology.


METHODS
mdr-1 gene expression of 46 untreated esophageal carcinoma was investigated with reverse transcription polymerase chain reaction (RT-PCR), and compared with the positive incidences among differentiated grades, TNM stages and macroscopic types of cancer.


RESULTS
All 46 samples were pathologically squamous cell carcinoma. The positive incidence of mdr-1 gene expression was 37% (17/46) in the whole group, and 35% (6/17), 40% (8/20), 33% (3/9) for differentiated grade I, II and III respectively. The expression rate of 33% (6/18), 40% (5/12), and 37% (6/16) was for TNM stage IIa, IIb, and III respectively. Macroscopically, the positive incidence was 37% (3/8) in constrictive, 33% (5/15) in fungating, 40% (6/14) in medullary, and 33% (3/9) in ulcerative type. There were no significant differences in each category system of morphology.


CONCLUSIONS
Because of highly expressive level of mdr-1 gene in untreated esophageal carcinoma, we should choose appropriate chemotherapeutic regimen for esophageal Ca. The expression of mdr-1 gene in untreated esophageal cancer was independent of morphologic prognostic indexes without correlation between mdr-1 gene expression and morphological indexes.
Chen
K
K
Department of Thoracic Surgery, First Affiliated Hospital, Hubei Medical University, Wuhan.
Xing
H
H
Cheng
B
B
D018435:ATP Binding Cassette Transporter, Subfamily B / Q000096:biosynthesis*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D019450:Genes, MDR; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging
D018435:ATP Binding Cassette Transporter, Subfamily B