P-Element repression in Drosophila melanogaster by a naturally occurring defective telomeric P copy.
In Drosophila melanogaster, hybrid dysgenesis occurs in progeny from crosses between females lacking P elements and males carrying P elements scattered throughout the genome. We have genetically isolated a naturally occurring P insertion at cytological location 1A, from a Tunisian population. The Nasr'Allah-P(1A) element [NA-P(1A)] has a deletion of the first 871 bp including the P promoter. It is flanked at the 3' end by telomeric associated sequences and at the 5' end by a HeT-A element sequence. The NA-P(1A) element strongly represses dysgenic sterility and P transposition. However, when testing P-promoter repression, NA-P(1A) was unable to repress a germinally expressed P-lacZ construct bearing no 5'-homology with it. Conversely, a second P-lacZ construct, in which the fusion with lacZ takes place in exon 3 of P, was successfully repressed by NA-P(1A). This suggests that NA-P(1A) repression involves a homology-dependent component.
Département Dynamique du Génome et Evolution, Institut Jacques Monod, 75251 Paris Cedex 05, France.
L
L
Marin
M
M
Lehmann
D
D
Nouaud
H
H
Izaabel
D
D
Anxolabéhère
S
S
Ronsseray
D000483:Alleles; D000818:Animals; D015152:Blotting, Northern; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005091:Exons; D005260:Female; D020868:Gene Silencing; D006059:Gonadal Dysgenesis / Q000235:genetics; D007763:Lac Operon; D008297:Male; D008957:Models, Genetic; D009154:Mutation; D010053:Ovary / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013489:Suppression, Genetic; D016615:Telomere / Q000235:genetics*; D013696:Temperature
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D012313:RNA