Characteristics and antifungal activity of a chitin binding protein from Ginkgo biloba.
An antifungal peptide from leaves of Ginkgo biloba, designated GAFP, has been isolated. Its molecular mass of 4244.0 Da was determined by mass spectrometry. The complete amino acid sequence was obtained from automated Edman degradation. GAFP exhibited antifungal activity towards Pellicularia sasakii Ito, Alternaria alternata (Fries) Keissler, Fusarium graminearum Schw. and Fusarium moniliforme. Its activities differed among various fungi. GAFP could also cause increased hyphal membrane permeabilization and a rapid alkalization of the medium when applied at 100 microgram/ml to Pellicularia sasakii Ito hyphae. The amino acid sequence of GAFP shows characteristics of the cysteine/glycine-rich chitin binding domain of many chitin binding proteins. The cysteine residues are well conserved.
Huang
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X
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 320 Yue-yang Road, 200031, Shanghai, PR China.
Xie
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W
Gong
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D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002686:Chitin / Q000378:metabolism*; D003545:Cysteine / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005658:Fungi / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D020441:Ginkgo biloba / Q000737:chemistry*; D005998:Glycine / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D010946:Plants, Medicinal; D011485:Protein Binding; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
D000935:Antifungal Agents; D002352:Carrier Proteins; D010455:Peptides; D010940:Plant Proteins; C412460:chitin-binding protein, Ginkgo biloba; D002686:Chitin; D003545:Cysteine; D005998:Glycine