Major house dust mite allergen, Der p I, activates phospholipase D in human peripheral blood mononuclear cells from allergic patients: involvement of protein kinase C.
The major house-dust-mite allergen, Der p I, stimulates the phospholipase D (PLD) in peripheral blood mononuclear cells (PBMC) from allergic patients with maximal responses after 30 min exposure. At 30 min, Der p I stimulated PLD activity by 1.4-fold in mild, 1.6-fold in moderate and 2-fold in severe allergic patients over control values (p < 0.05). When the cells were pretreated for 24 h with phorbol myristate acetate to down-regulate protein kinase C (PKC), PLD stimulation by Der p I was largely abolished. These results indicate that in PBMC from allergic patients, Der p I can stimulate PLD activity, and that PKC activation is involved in this stimulation.
Department of Pediatrics, College of Medicine, Hanyang University, Seoul, Korea.
J W
JW
Oh
J H
JH
Kim
K S
KS
Lee
J S
JS
Han
D000328:Adult; D000485:Allergens / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D039741:Antigens, Dermatophagoides; D015536:Down-Regulation; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008925:Mites / Q000276:immunology / Q000378:metabolism; D010739:Phospholipase D / Q000276:immunology / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D012882:Skin Tests; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007073:Immunoglobulin E; D011493:Protein Kinase C; D010739:Phospholipase D; D013755:Tetradecanoylphorbol Acetate