Inhibition of tetanus toxin fragment C binding to ganglioside G(T1b) by monoclonal antibodies recognizing different epitopes.
Anti-tetanus toxoid monoclonal antibodies would be useful in exploring the relationship of tetanus toxin structure to its function. Tetanus toxin fragment C has been shown to be responsible for binding to neurons via gangliosides. Eleven new and two previously derived monoclonal antibodies specific for tetanus toxin fragment C were shown to recognize five different fragment C epitopes, two of which were overlapping. Three of these epitopes participate in the binding to ganglioside G(T1b). One epitope was defined by a monoclonal antibody that did not inhibit the interaction between fragment C and ganglioside. This antibody however, was blocked from binding to fragment C by antibodies that were able to inhibit the fragment C-ganglioside interaction.
Fitzsimmons
S P
SP
Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852, USA.
Clark
K C
KC
Wilkerson
R
R
Shapiro
M A
MA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D001667:Binding, Competitive / Q000187:drug effects; D003429:Cross Reactions; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D005260:Female; D005732:Gangliosides / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013744:Tetanus Toxin / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D005732:Gangliosides; D010446:Peptide Fragments; D013744:Tetanus Toxin; C040635:tetanus toxin fragment C; C016481:trisialoganglioside GT1