Osteoblastic networks with deficient coupling: differential effects of magnetic and electric field exposure.
A gap junction-deficient cell line was utilized to test whether intercellular coupling plays a significant role in modulating the influence of biophysical stimuli such as extracellular electrical currents. ROS 17/2.8 cells, an osteosarcoma cell line, along with a control transfected cell line and a connexin 43-gap junction-deficient cell line, were exposed to a time-changing magnetic flux (30 Hz, 1.8 milliTesla) sufficient to induce an electric field in the cultures on the order of 2 mV/m. Field exposure inhibited cell growth independent of gap junctional coupling, while alkaline phosphatase activity was found to be dependent on gap junctional coupling. These findings can be interpreted to suggest that magnetic and electric field exposures have differential effects on cell cultures, with magnetic field exposure inhibiting cell growth through a mechanism independent of gap junctional coupling, while the alteration in enzyme activity appears to be stimulated by the induced electric field in a gap junction-dependent manner.
Musculo-Skeletal Research Laboratory, Program in Bioengineering, State University of New York at Stony Brook, Stony Brook, NY 11794-5281, USA.
M A
MA
Vander Molen
H J
HJ
Donahue
C T
CT
Rubin
K J
KJ
McLeod
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002450:Cell Communication / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D018031:Connexin 43 / Q000235:genetics; D004574:Electromagnetic Fields; D017629:Gap Junctions / Q000502:physiology*; D015870:Gene Expression / Q000502:physiology; D010006:Osteoblasts / Q000737:chemistry / Q000166:cytology* / Q000201:enzymology; D012516:Osteosarcoma; D051381:Rats; D014162:Transfection; D014407:Tumor Cells, Cultured
D018031:Connexin 43; D000469:Alkaline Phosphatase