[Alteration of fatty acid components of erythrocyte membrane and insulin resistance in diabetic patients].
OBJECTIVE
To study the effect of alteration of erythrocyte membrane fatty acid components and fluidity on insulin resistance (ISR) in NIDDM patients.


METHODS
The fatty acid(FA) components and fluidity of erythrocyte membrane in 52 NIDDM patients with and without hyper-insulinemia were examined by a high-performance liquid chromatographic method and flourescence polarization technique.


RESULTS
The content and composition erythrocyte membrane arachidonate (AA) were significantly lower in NIDDM patients than in controls, and also markedly lower in patients with hyperinsulinemia than those without hyperinsulinemia. The diabetics had increased erythrocyte membrane microviscosities as compared with controls, and membrane microviscosities were higher in diabetics with hyperinsulinemia than those without hyperinsulinemia. AA contents of erythrocyte membrane were negatively correlated with membrane membrane microviscosities and positively with ISI in NIDDM patients.


CONCLUSIONS
In NIDDM patients, hyperinsulinemia and ISI bring about AA metabolic abnormalities and decreased AA contents in cell membrane. Alteration of cell membrane FA components results in the changes of membrane fluidity, and the latter can increase IRS.
Yang
G
G
Department of Internal Medicine, First Affiliated Hospital, Chongqing Medical University.
Li
L
L
Dang
H
H
D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged
D005227:Fatty Acids