Evaluation of immunomagnetic separation for recovery of Cryptosporidium parvum and Giardia duodenalis from high-iron matrices.
In this study we examined the recovery of Cryptosporidium parvum and Giardia duodenalis from matrices containing various concentrations of dissolved iron. The organisms were recovered by using the immunomagnetic separation-immunofluorescent assay method, and the levels of recovery were compared to the dissolved iron concentrations. The levels of recovery of C. parvum decreased sharply at dissolved iron concentrations greater than 4 mg/liter, while the levels of recovery of G. duodenalis decreased sharply at concentrations greater than 40 mg/liter.
Allegheny County Sanitary Authority, Pittsburgh, Pennsylvania 15233, USA.
G P
GP
Yakub
K L
KL
Stadterman-Knauer
D000818:Animals; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D004492:Edetic Acid; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D005872:Giardia / Q000302:isolation & purification*; D018189:Immunomagnetic Separation / Q000379:methods*; D007501:Iron / Q000737:chemistry*; D008851:Micropore Filters
D004492:Edetic Acid; D007501:Iron