Construction of mutants of Sphingomonas paucimobilis defective in terminal mannose in the glycosphingolipid.
Mutants of Sphingomonaspaucimobilis defective in a part of the carbohydrate moiety of the glycosphingolipid (GSL) were constructed by transposon (Tn5)-insertional mutagenesis. Defective mutants were selected by ELISA using the antibody recognizing the tetrasaccharide-type GSL (GSL-4A) of S. paucimobilis. Eight defective mutants were selected from about 8,000 kanamycin-resistant strains, and seven of them were found to lack the terminal mannose of GSL-4A. The chemical structure of the mutant GSL was investigated, and proved that the rest of the structure was not changed by the mutation.
Kaneko
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A
Department of Microbiology, School of Pharmaceutical Sciences,The Kitasato Institute, Kitasato University, Tokyo, Japan.
Miyadai
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H
Danbara
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H
Kawahara
K
K
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006028:Glycosphingolipids / Q000737:chemistry* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020578:Sphingomonas / Q000737:chemistry* / Q000235:genetics*
D002241:Carbohydrates; D005227:Fatty Acids; D006028:Glycosphingolipids; D008358:Mannose