Use of mixed anhydrides for the determination of terfenadine in dosage forms and spiked human plasma.
Terfenadine reacts with mixed anhydrides (malonic and acetic anhydrides) producing a yellow-coloured product with intense fluorescence. Based on this fact, a spectrophotometric method was developed for the determination of terfenadine in dosage forms. The relation between the absorbance at 395 nm and the concentration is rectilinear over the range 0.5-5 microg ml(-1) (molar absorptivity is 1.405 x 10(5) l mol(-1) cm (-1)). The reaction product was also measured spectrofluorimetrically at 435 nm after excitation at 395 nm. The fluorescence intensity was directly proportional to the concentration over the range 0.5-4 ng ml(-1) with minimum detectability (S/N = 2) of 0.07 microg ml(-1) (approximately 1.5 x 10(-10) M). The different parameters affecting the development and stability of the reaction product were carefully studied and incorporated into the procedure. The proposed spectrophotometric method was successfully applied to the determination of terfenadine in tablets and suspensions; the percentage recoveries were 99.83 +/- 0.75 and 99.65 +/- 0.83, respectively. The proposed spectrofluorimetric method was applied to the determination of terfenadine in spiked human plasma. The percentage recovery was 99.35 +/- 2.19. The method is highly sensitive and specific. No interference was noticed from co-formulated drugs, such as pseudoephedrine and ibuprofen.
Al-Majed
A A
AA
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. almajed99@yahoo.com
Al-Zehouri
J
J
Belal
F
F
D000812:Anhydrides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004304:Dosage Forms; D006635:Histamine H2 Antagonists / Q000032:analysis* / Q000097:blood; D006801:Humans; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D016593:Terfenadine / Q000032:analysis* / Q000097:blood
D000812:Anhydrides; D004304:Dosage Forms; D006635:Histamine H2 Antagonists; D004364:Pharmaceutical Preparations; D016593:Terfenadine