[The effect of IL-2 and IL-3 gene therapy in mice on immunological recovery receiving high dose chemotherapy].
OBJECTIVE
To investigate the effect of Ad-IL-2 and Ad-IL-3 on immunological recovery.


METHODS
The Ad-IL-2 and/or Ad-IL-3 were i.p. injected into C57BL/6 mice having received high dose cyclophosphamide (200 mg/kg) 24 hours before. The number and cytotoxicity, Ia antigen expression of peritoneal macrophages, the proliferation and NK cell activity of splenic cells were observed.


RESULTS
After i.p. injection with Ad-IL-3, the number, cytotoxicity and Ia antigen expression of peritoneal macrophagee were increased. After i.p. injection with Ad-IL-2, the proliferation and NK cell activity of splenic cells were significantly augmented. As i.p. injection with Ad-IL-2 and Ad-IL-3 simultaneously, both the peritoneal macrophages and splenic cells were activated.


CONCLUSIONS
Intraperitoneal injection of Ad-IL-2 and Ad-IL-3 can promote immune recovery after high dose chemotherapy.
Department of Hematology, Changhai Hospital.
M
M
Yu
X
X
Cao
G
G
Lou
D000256:Adenoviridae; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage / Q000494:pharmacology; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D015316:Genetic Therapy; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D007939:Leukemia L1210 / Q000276:immunology* / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D018906:Antineoplastic Agents, Alkylating; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D007377:Interleukin-3; D011994:Recombinant Proteins; D003520:Cyclophosphamide