Clinical pregnancy following blastomere biopsy and PGD for a reciprocal translocation carrier: analysis of meiotic outcomes and embryo quality in two IVF cycles.
A couple were referred for preimplantation genetic diagnosis (PGD) following diagnosis of a reciprocal translocation in the female partner: 46,XX,t(14;22)(q11.2;q13.3). PGD was carried out using fluorescence in situ hybridization (FISH) with probes specific for the translocated and centric segments of chromosome 22. An initial cycle was unsuccessful, producing 11 embryos for biopsy, only one of which, when followed up on day 4, yielded more than 10 nuclei (median 7.5, n=10). In addition, five of the embryos showed mosaic or chaotic chromosome constitutions; some of these embryos had fragmented or multilobed abnormal nuclei, hindering interpretation of the FISH signals. The single embryo transferred did not result in a pregnancy. A second cycle, using a revised protocol, produced 10 embryos, three of which were transferred, resulting in an ongoing singleton pregnancy. All the remaining embryos yielded 12 to 23 nuclei by day 4 (median 17, n=7). Apart from some tetraploid nuclei, only one embryo showed mosaicism. The significance of the changes in protocol leading to the successful outcome is discussed, and the pattern of meiotic segregation products is analysed and compared with other previous reports of reciprocal translocations.
Cytogenetics Department, Division of Medical and Molecular Genetics, Guy's Tower, St. Thomas' Street, London SE1 9RT, UK. p.scrivn@umds.ac.uk
P N
PN
Scriven
F
F
O'Mahony
H
H
Bickerstaff
C T
CT
Yeong
P
P
Braude
C
C
Mackie Ogilvie
D000328:Adult; D001706:Biopsy; D001757:Blastomeres / Q000473:pathology*; D002883:Chromosomes, Human, Pair 14; D002892:Chromosomes, Human, Pair 22; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006580:Genetic Carrier Screening / Q000379:methods; D030342:Genetic Diseases, Inborn / Q000175:diagnosis* / Q000517:prevention & control; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008540:Meiosis / Q000502:physiology; D011247:Pregnancy; D019836:Preimplantation Diagnosis; D014178:Translocation, Genetic