Inactivation of phytotoxin produced by the rice sheath blight pathogen Rhizoctonia solani.
The rice sheath blight pathogen, Rhizoctonia solani, produces a toxin designated as RS-toxin, a carbohydrate compound containing mainly alpha-glucose and mannose. Different microflora were tested for RS-toxin inactivation. Isolates of Trichoderma viride inactivated this toxin when it was provided as the sole food source, and these isolates reduced the severity of toxin-induced symptoms and electrolyte leakage from rice cells. The best-performing isolate, TvMNT7, produced two extracellular proteins of 110 and 17 kDa. The high molecular mass protein was shown to have alpha-glucosidase activity. The purified 110 kDa protein was able to reduce RS-toxin activity.
Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India.
S
S
Sriram
T
T
Raguchander
S
S
Babu
R
R
Nandakumar
V
V
Shanmugam
P
P
Vidhyasekaran
P
P
Balasubramanian
R
R
Samiyappan
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009183:Mycotoxins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D012232:Rhizoctonia / Q000378:metabolism* / Q000472:pathogenicity; D014242:Trichoderma / Q000302:isolation & purification / Q000378:metabolism; D000520:alpha-Glucosidases / Q000737:chemistry / Q000378:metabolism
D005656:Fungal Proteins; D009183:Mycotoxins; D000520:alpha-Glucosidases