In vitro modulation of fish phagocytic cells by beta-endorphin.
The activation of rainbow trout, Oncorhynchus mykiss, and carp, Cyprinus carpio, phagocytic cells by synthetic chum salmon, O. keta, beta-endorphin was analysed in vitro. Rainbow trout head kidney leukocytes were cultured in RPMI 1640 medium containing 1, 10, 50 or 100 ng ml-1 of chum salmon beta-endorphin and the production of superoxide anion was measured via the reduction of nitroblue tetrazolium (NBT) in vitro. Macrophages incubated with 10 ng ml-1 up to 100 ng ml-1 of beta-endorphin showed an increase in their production of superoxide anion in comparison with control macrophages which were cultured without hormone. beta-endorphin also increased the production of superoxide anion in phagocytic cells prepared from kidney of carp. This stimulation was inhibited by naloxone. Phagocytic cells treated with beta-endorphin also displayed increased phagocytic activity and phagocytic index. These results showed that beta-endorphin in lower vertebrates activates the function of phagocytic cells in vitro.
Faculty of Agriculture, Miyazaki University, Japan.
H
H
Watanuki
Y
Y
Gushiken
A
A
Takahashi
A
A
Yasuda
M
M
Sakai
D000595:Amino Acid Sequence; D000818:Animals; D002347:Carps / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology; D007962:Leukocytes / Q000276:immunology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008969:Molecular Sequence Data; D009270:Naloxone / Q000494:pharmacology; D017685:Oncorhynchus keta / Q000276:immunology*; D017686:Oncorhynchus mykiss / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism; D001615:beta-Endorphin / Q000494:pharmacology*
D013481:Superoxides; D009270:Naloxone; D001615:beta-Endorphin