Urine protein analysis by gel electrophoresis and laser densitometry after chemotherapy in pediatric cancer patients.
A common side effect of chemotherapy is reversible or nonreversible nephrotoxicity. SDS polyacrylamide gel electrophoresis combined with laser densitometry was evaluated as a suitable method to analyze pathologic urine proteins. A total of 52 pediatric patients were followed during and 63 patients were followed after therapy. During therapy renal damage was recorded in 43% of the leukemia patients, in 56% of nephroblastoma patients, and 75% of patients with other tumors. Three or more months after therapy pathologic patterns were seen in 25% of acute lymphoblastic leukemia patients, in 35% of patients with nephroblastoma, and in 62% of other patients. Patients with persistent complete tubular proteinuria and mixed glomerular/tubular proteinuria were found to have a high risk for irreversible renal damage and should be controlled periodically. This method permits a rapid and reliable analysis of urine proteins and is suitable for follow-up tests of renal function during and after chemotherapy.
Department of Pediatrics, Technical University Rheinisch-Westfälische Technische Hochschule, Aachen, Germany. rmertens@post.klinikum.rwth-aachen.de
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R
Mertens
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Granzen
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Vogt
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Melzer
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Mann
D000293:Adolescent; D000970:Antineoplastic Agents / Q000009:adverse effects* / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D003720:Densitometry / Q000379:methods / Q000592:standards; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000592:standards; D006801:Humans; D007678:Kidney Glomerulus / Q000737:chemistry; D007684:Kidney Tubules / Q000737:chemistry; D007834:Lasers; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications / Q000188:drug therapy* / Q000652:urine; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D011507:Proteinuria / Q000139:chemically induced / Q000175:diagnosis* / Q000378:metabolism; D051437:Renal Insufficiency / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism; D009396:Wilms Tumor / Q000150:complications / Q000188:drug therapy* / Q000652:urine
D000970:Antineoplastic Agents; D011506:Proteins