Nucleotide sequence of alpha-galactosidase MEL gene from Zygosaccharomyces mrakii.
The region encompassing the alpha-galactosidase MELr gene was amplified from Zygosaccharomyces mrakii IFO 1835(T) by inverse-PCR and then sequenced. The nucleotide sequence of this region revealed a single open reading frame of 1410 bp encoding a 470 amino acid protein with a molecular weight of 51,909. The similarity of the deduced mature protein to other yeast alpha-galactosidases was 63.3% to Zygosaccharomyces cidri, 71.5% to Torulaspora delbrueckii, and 70. 7-73.9% to Saccharomyces species. The nucleotide and amino acid sequences are deposited in the DDBJ/EMBL/GenBank database under Accession Number AB030209.
Oda
Y
Y
Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Memuro, Kasai, Hokkaido, 082-0071, Japan. yujioda@mmr.affrc.go.jp
Fujisawa
T
T
D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D020068:Zygosaccharomyces / Q000201:enzymology / Q000235:genetics*; D000519:alpha-Galactosidase / Q000737:chemistry / Q000235:genetics*
D005656:Fungal Proteins; D000519:alpha-Galactosidase