Mechanistic studies on prolyl-4-hydroxylase: the vitamin C requiring uncoupled oxidation.
A deuterated substrate for the human type I prolyl-4-hydroxylase was synthesized and its V/K deuterium isotope effect was determined to be 3.4 +/- 0.2. This isotope effect was attributed to the uncoupled oxidation. A dehydroproline containing tetrapeptide was also found to stimulate the uncoupled oxidation.
Wu
M
M
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Moon
H S
HS
Pirskanen
A
A
Myllyharju
J
J
Kivirikko
K I
KI
Begley
T P
TP
D000595:Amino Acid Sequence; D001205:Ascorbic Acid / Q000378:metabolism*; D003903:Deuterium; D006801:Humans; D007700:Kinetics; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011347:Procollagen / Q000737:chemistry / Q000378:metabolism; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D011392:Proline; D013379:Substrate Specificity
D009842:Oligopeptides; D011347:Procollagen; D011392:Proline; D003903:Deuterium; D011393:Procollagen-Proline Dioxygenase; D001205:Ascorbic Acid