A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division.
We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.
Rogers
G C
GC
Section of Molecular and Cellular Biology, University of California at Davis, Davis, California 95616, USA.
Chui
K K
KK
Lee
E W
EW
Wedaman
K P
KP
Sharp
D J
DJ
Holland
G
G
Morris
R L
RL
Scholey
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D018797:Cell Cycle Proteins / Q000235:genetics; D019281:Dimerization; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D016547:Kinesins / Q000235:genetics; D008677:Metaphase; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000302:isolation & purification; D008954:Models, Biological; D020409:Molecular Motor Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics / Q000302:isolation & purification*; D012617:Sea Urchins; D017386:Sequence Homology, Amino Acid; D008941:Spindle Apparatus / Q000648:ultrastructure*; D029867:Xenopus Proteins
D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; C097723:KIF15 protein, Xenopus; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D009124:Muscle Proteins; D029867:Xenopus Proteins; C074152:kinesin-II; D016547:Kinesins