[Construction of PCNA antisense RNA expression vector and its antitumorigenic effect on human gastric cancer cell].
OBJECTIVE
To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.


METHODS
An eukaryotic expression vector pDR-PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC-7901 was transfected with pDR-PCNA by lipofectamine.


RESULTS
Growth rate, protein and RNA biosynthesis and DNA contents in S-phase and G2/M-phase of the transfected SGC-7901 were significantly suppressed in comparison with those of the parental cell line.


CONCLUSIONS
PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.
Dai
L
L
Department of Gastroenterology, 210 Hospital of PLA, Dalian.
Zhang
X
X
Hui
H
H
D002455:Cell Division; D004273:DNA, Neoplasm / Q000378:metabolism; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics*; D016372:RNA, Antisense / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen; D016372:RNA, Antisense