Sequence variation of new alleles at the short tandem repeat D19S253 locus.
This paper reports the sequences of two new alleles identified in a population database study on the short tandem repeat D19S253 locus. A Portuguese Caucasian population and a Portuguese African population were studied. Forty-four selected alleles were sequenced and 11 different alleles were found. All the sequenced alleles shown to possess a simple tetranucleotide GATA repeat region structure. The two new alleles, alleles 6 and 16, follow the simple repeat pattern. During paternity investigation casework, 1028 meiosis were analyzed and five isolated genetic incompatibilities detected. In one case, a non-detectable allele with the used set of primers could be the explanation. In the other four cases, single-step mutations could be considered. The mutation rate obtained for this locus was 3.89 x 10(-3).
Forensic Biology Laboratory, Institute of Legal Medicine, Lisbon, Portugal.
R M
RM
Brito
T
T
Ribeiro
L
L
Viriato
C
C
Vieira-Silva
R
R
Espinheira
I
I
Pinto-Ribeiro
H
H
Geada
D000483:Alleles; D044383:Black People / Q000235:genetics; D016172:DNA Fingerprinting; D004252:DNA Mutational Analysis; D016208:Databases, Factual; D005828:Genetics, Population; D006801:Humans; D010334:Paternity; D020080:Tandem Repeat Sequences / Q000235:genetics*; D044465:White People / Q000235:genetics