Effects of the Calvin cycle on nicotinamide adenine dinucleotide concentrations and redox balances of Xanthobacter flavus.
The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for excess reducing equivalents. The physiological data support the conclusion previously derived from molecular studies in that expression of the Calvin cycle genes is controlled by the intracellular concentration of NADPH.
van Keulen
G
G
Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9750 AA Haren, The Netherlands.
Dijkhuizen
L
L
Meijer
W G
WG
D005560:Formate Dehydrogenases / Q000378:metabolism; D007700:Kinetics; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D015219:Substrate Cycling; D020584:Xanthobacter / Q000254:growth & development / Q000378:metabolism*
D009243:NAD; D009249:NADP; D005560:Formate Dehydrogenases; D010735:Phosphoglycerate Kinase; D012273:Ribulose-Bisphosphate Carboxylase