[Antitumor effect of M-CSF and/or IFN-gamma gene cotransfected-macrophages by intratumoral injection].
OBJECTIVE
To investigate the in vivo antitumor effects of M-CSF and/or IFN-gamma gene transfected-macrophages and their related immunological mechanisms.


METHODS
Survival time and antitumor effects in vivo of tumor-bearing mice treated with gene-transfected macrophages were observed. NK cell and CTL activity was detected with 4 hr 51Cr release assay, cytotoxicity of macrophages was detected with indirect MTT methods, activity of IL-2, TNF, IFN-gamma were detected by routine methods.


RESULTS
25% of the tumor-bearing mice after treatment with IFN-gamma gene-transfected, M-CSF and IFN-gamma gene co-transfected macrophages survived more than 3 month. The CTL and macrophage activity of the tumor-bearing mice was enhanced. Levels of TNF, IL-2, IFN-gamma in the supernatants of splenocytes induced in vitro were also increased. Histologic analyses of the tumor tissue after treatment with M-CSF and/or IFN-gamma gene-transfected macrophages showed a large amount of lymphocyte infiltration.


CONCLUSIONS
IFN-gamma and M-CSF gene co-transfection into macrophages are proved to enhance their antitumor potential efficiently through direct augmentation of their tumoricidal cytotoxicity and induction of host antitumor immunity.
Lei
H
H
Department of Immunology, Second Medical Military University, Shanghai.
Cao
X
X
Yu
Y
Y
D000818:Animals; D015316:Genetic Therapy; D007371:Interferon-gamma / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D008264:Macrophages / Q000276:immunology*; D008546:Melanoma, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011897:Random Allocation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection
D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma