Assessment of primary hemostasis by PFA-100 analysis in a tertiary care center.
We evaluated the utility of the PFA-100 platelet function analyzer in identifying disorders in platelet function and/or von Willebrand factor (vWF) in patients with various systemic disorders being followed at a tertiary care center. Closure times were determined with collagen/ ADP (CADP) and collagen/epinephrine (CEPI) cartridges for 305 patients, and abnormal results were further evaluated with platelet aggregometry and vWF analysis. Prolonged CADP and/or CEPI closure times were identified in 114 patients (37.3%), but most were isolated prolonged CEPI closure times predominantly due to aspirin therapy (79 patients). Prolonged CADP closure times were most frequently due to qualitative platelet defects and/or decreased vWF levels. Prolonged CADP closure times were encountered most frequently in patients with sickle cell disease and were associated with a decreased hematocrit. This study demonstrated that the PFA-100 analyzer can accurately assess vWF-dependent platelet function and detect other platelet defects under high shear stress in complex patient populations.
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA. orte001@mc.duke.edu
T L
TL
Ortel
A H
AH
James
E H
EH
Thames
K D
KD
Moore
C S
CS
Greenberg
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000740:Anemia / Q000097:blood; D000755:Anemia, Sickle Cell / Q000097:blood; D001791:Blood Platelet Disorders / Q000097:blood / Q000175:diagnosis*; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D005500:Follow-Up Studies; D006400:Hematocrit; D006785:Hospitals, University; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D009657:North Carolina / Q000453:epidemiology; D010044:Outpatient Clinics, Hospital; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation; D010979:Platelet Function Tests / Q000295:instrumentation*; D011247:Pregnancy; D013314:Stress, Mechanical; D013997:Time Factors; D014842:von Willebrand Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology
D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine