Aromatase activity during embryogenesis in the brain and adrenal-kidney-gonad of the red-eared slider turtle, a species with temperature-dependent sex determination.
Gonadal sex in the red-eared slider turtle is determined by the incubation temperature that the embryo experiences during the mid-trimester of development. High temperatures result in female-biased sex ratios, and low temperatures produce male-biased sex ratios. The physiological equivalent of temperature appears to be a combination of the nature and abundance of steroidogenic enzymes and their products-including estradiol and its precursor, testosterone-and aromatase, the enzyme that converts testosterone to estradiol. Aromatase has been hypothesized to play a major role in the female developmental pathway in this species, and research in other species with temperature-dependent sex determination points to the brain as an organ that transduces the temperature signal into an aromatase response. In this study, we used a tritiated water assay to compare the pattern of estradiol biosynthesis at male- and female-producing temperatures in the brain and adrenal-kidney-gonad (AKG) through development. The pattern for both sexes in the AKG was one of increased activity after the temperature-sensitive period (TSP), but with no significant difference between sexes. In the brain, however, putative females exhibited a significantly higher level of aromatase activity than putative males at the beginning of the TSP, after which activity in both male and female brains decreased, dropping below detection in females before hatch. These results point to the brain as a site of aromatase response to temperature in this species, and they suggest that the product of aromatase activity, estradiol, may induce alterations in the neuroendocrine axis controlling gonadal sex steroid hormone production.
Institute of Reproductive Biology, University of Texas at Austin, Austin, Texas, 78712, USA.
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E
Willingham
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Baldwin
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JK
Skipper
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D
Crews
D000311:Adrenal Glands / Q000196:embryology* / Q000201:enzymology; D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D001921:Brain / Q000196:embryology* / Q000201:enzymology; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006066:Gonads / Q000196:embryology* / Q000201:enzymology; D007668:Kidney / Q000196:embryology* / Q000201:enzymology; D008297:Male; D019849:Sex Determination Processes; D013696:Temperature; D014316:Tritium; D014426:Turtles / Q000196:embryology*
D014316:Tritium; D004958:Estradiol; D001141:Aromatase