Trichomonas vaginalis: analysis of a heat-inducible member of the cytosolic heat-shock-protein 70 multigene family.
A 2253-nucleotide (nt) transcript for a Trichomonas vaginalis heat-shock protein 70, TVCHSP70, has been isolated that encodes for a protein of 659 amino acids with a predicted molecular weight of 71.3 kDa. TVCHSP70 has a short (10-nt) 5' untranslated region (UTR), and the 263-nt 3' UTR is the longest reported for a Trichomonas peptide. Amino-acid sequence analysis and phylogenetic comparison identifies TVCHSP70 as a member of the heat-inducible cytoplasmic HSP70 gene family. Southern-blot data indicate that T. vaginalis contains at least four members of the cytoplasmic HSP70 gene family. Members of the TVCHSP70 family are expressed as 2.3-kb transcripts at low levels during 37 degrees C culture, and their expression is significantly up-regulated at 43 degrees C. Slot-blot analysis of seven T. vaginalis clinical isolates demonstrated a 3- to 44-fold up-regulation of TVCHSP70 under conditions of heat shock (43 degrees C) or oxidative stress (500 microm H2O2) as compared with controls (37 degrees C).
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505, USA.
S R
SR
Davis-Hayman
P H
PH
Shah
R W
RW
Finley
W B
WB
Lushbaugh
J C
JC
Meade
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003600:Cytosol / Q000378:metabolism; D016054:DNA, Protozoan; D017125:Genes, Protozoan; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D005810:Multigene Family; D012333:RNA, Messenger; D016053:RNA, Protozoan / Q000032:analysis; D014246:Trichomonas vaginalis / Q000235:genetics*
D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan