Multiplex PCR for the identification of white Tuber species.
Species-specific primers selected from the internal transcribed spacer region sequence were used to set up a multiplex polymerase chain reaction (PCR) able to simultaneously identify the white truffle species Tuber magnatum, Tuber borchii, Tuber maculatum and Tuber puberulum. Furthermore, a primer specific for the competitive fungus Sphaerosporella brunnea was designed and added to the multiplex PCR set, allowing the detection of the Tuber species and the contaminant fungus in a one-step reaction.
Amicucci
A
A
Istituto di Chimica Biologica Giorgio Fornaini, Univerità degli Studi di Urbino, Italy.
Guidi
C
C
Zambonelli
A
A
Potenza
L
L
Stocchi
V
V
D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016681:Genome, Fungal; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods