Pyrolysis/gas chromatography/mass spectrometry monitoring of fungal-biotreated distillery wastewater using Trametes sp. I-62 (CECT 20197).
Distillery wastewaters generated by ethanol production from fermentation of sugar-cane molasses, named vinasses, lead to important ecological impact due to their high content of soluble organic matter and their intense dark-brown color. Taking advantage of the well-known ability of white-rot fungi to degrade an extensive variety of organic pollutants, the capacity of Trametes sp. I-62 (CECT 20197) to detoxify this type of effluents was evaluated. In this work, pyrolysis/gas chromatography/mass spectrometry was applied to the chemical characterization of several fractions of Cuban distillery wastewater as well as to monitoring the changes which occurred after fungal treatment with this white-rot basidiomycete. Maximum effluent decolorization values and chemical oxygen demand reduction attained after seven days of fungal treatment were 73.3 and 61.7%, respectively, when 20% (v/v) of distillery vinasses was added to the culture medium. Under these conditions a 35-fold increase in laccase production by Trametes sp. I-62 was measured, but no manganese peroxidase activity could be detected. The pyrolysis/gas chromatography/mass spectrometry results showed a decrease in a number of pyrolysis products after seven days of fungal treatment, mainly furan derivatives. The decrease in the relative areas of these compounds could be related to the vinasse color-removal associated with melanoidin degradation. All these results indicated the potential use ofTrametes sp. I-62 in the detoxification of recalcitrant distillery vinasses.
ICIDCA, Instituto Cubano de los Derivados de la Caña de Azúcar. Via Blanca 804 esq. Carretera Central, Apdo. 4026. C.P. 11000. La Habana, Cuba.
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González
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Terrón
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Yagüe
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Zapico
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GC
Galletti
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González
D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D007220:Industrial Waste / Q000032:analysis*; D042845:Laccase; D010088:Oxidoreductases / Q000032:analysis; D010100:Oxygen / Q000032:analysis; D011124:Polyporaceae / Q000737:chemistry*; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000032:analysis*
D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D010088:Oxidoreductases; D042845:Laccase; D010100:Oxygen