Light scattering from cells: the contribution of the nucleus and the effects of proliferative status.
As part of our ongoing efforts to understand the fundamental nature of light scattering from cells and tissues, we present data on elastic light scattering from isolated mammalian tumor cells and nuclei. The contribution of scattering from internal structures and in particular from the nuclei was compared to scattering from whole cells. Roughly 55% of the elastic light scattering at high-angles (> 40 degrees) comes from intracellular structures. An upper limit of 40% on the fractional contribution of nuclei to scattering from cells in tissue was determined. Using cell suspensions isolated from monolayer cultures at different stages of growth, we have also found that scattering at angles greater than about 110 degrees was correlated with the DNA content of the cells. Based on model calculations and the relative size difference of nuclei from cells in different stages of growth, we argue that this difference in scattering results from changes in the internal structures of the nucleus. This interpretation is consistent with our estimate of 0.2 micron as the mean size of the scattering centers in cells. Additionally, we find that while scattering from the nucleus accounts for a majority of internal scattering, a significant portion must result from scattering off of cytoplasmic structures such as mitochondria.
Los Alamos National Laboratory, Bioscience Division, New Mexico 87545, USA.
J R
JR
Mourant
M
M
Canpolat
C
C
Brocker
O
O
Esponda-Ramos
T M
TM
Johnson
A
A
Matanock
K
K
Stetter
J P
JP
Freyer
D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis; D005347:Fibroblasts / Q000648:ultrastructure*; D011905:Genes, ras / Q000235:genetics; D008027:Light; D009374:Neoplasms, Experimental / Q000175:diagnosis; D017354:Point Mutation; D051381:Rats; D011916:Rats, Inbred F344; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm