Effect of IOP elevation on matrix metalloproteinase-2 in rabbit anterior chamber.
To investigate changes in the level of matrix metalloproteinase-2 (MMP-2) in the anterior chamber of rabbit with intraocular pressure (IOP) elevation. The IOP was elevated with scleral encircling in 12 rabbits. In the control group (4 rabbits), IOP was not changed after scleral encircling, and in group 1 (4 rabbits) and 2 (4 rabbits), IOP was elevated about 10 and 20 mmHg respectively after scleral encircling. At 2 days after scleral encircling, aqueous sampling was performed and levels of MMP-2 were checked by Western blots and gelatin zymograms. The greater the IOP elevation, the more MMP-2 expression in the anterior chamber by Western blots and gelatin zymograms. The increase in MMP-2 expression in response to IOP elevation may have important implications for the IOP feedback control mechanism.
Department of Ophthalmology, Yonsei University, College of Medicine, Seoul, Korea.
C Y
CY
Kim
S S
SS
Kim
H J
HJ
Koh
Y S
YS
You
G J
GJ
Seong
Y J
YJ
Hong
D000818:Animals; D000867:Anterior Chamber / Q000201:enzymology*; D001082:Aqueous Humor / Q000201:enzymology*; D015415:Biomarkers; D015153:Blotting, Western; D007429:Intraocular Pressure; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009798:Ocular Hypertension / Q000201:enzymology* / Q000209:etiology; D011817:Rabbits; D012590:Sclera / Q000601:surgery; D015503:Surgical Sponges / Q000009:adverse effects
D015415:Biomarkers; D020778:Matrix Metalloproteinase 2