IL-1beta induced changes in hypothalamic IL-1R1 and IL-1R2 mRNA expression in the rat.
Interleukin-1 receptor (IL-1R1 and IL-1R2) mRNA expression was detected within the rat hypothalamus, a primary site of IL-1 action, using RT-PCR. Levels of expression were unchanged by cardiac saline-perfusion. However, intracerebroventricular (i.c.v.) administration of IL-1beta caused changes in receptor mRNA expression in non-perfused animals that were profoundly different to those observed in their saline-perfused counterparts. This study demonstrates the importance of perfusing tissue to remove blood cells when determining changes in IL-1 receptor mRNA expression.
School of Biological Sciences, The University of Manchester, UK.
L C
LC
Parker
D A
DA
Rushforth
N J
NJ
Rothwell
G N
GN
Luheshi
D000818:Animals; D002552:Cerebral Ventricles / Q000187:drug effects / Q000502:physiology; D007031:Hypothalamus / Q000187:drug effects / Q000276:immunology*; D007276:Injections, Intraventricular; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects* / Q000276:immunology; D015854:Up-Regulation / Q000187:drug effects*
D007375:Interleukin-1; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1