[Study on combined chemosensitivity test in acute non-lymphocytic leukemia].
OBJECTIVE
To explore the methods of combined chemosensitivity test in vitro.


METHODS
The chemosensitivity of leukemia cells to 7 kinds of combined chemotherapy regimens, including DA, HA, AA, MA, HAD, HAA and HAM, was estimated in 79 patients with ANLL by MTT assay in vitro. The ratios of drug combinations at which synergistic interactions between drugs are to the highest levels were determined by using Chou's "median effect analysis".


RESULTS
There were 67 S(sensitive)/S, 9 R(resistant)/R, 2 R/S, 1 S/R(in vitro/in vivo) in 79 patients. The general, positive and negative coincident rates of in vitro with in vivo, specificity and sensitivity were 96.2%, 98.5%, 81.8%, 88.9% and 97.1%, respectively.


CONCLUSIONS
This method is better than the single drug test, and it is more useful to find drug resistant patients.
Institute of Hematology, CAMS, Tianjin.
M
M
Luo
S
S
Bian
Y
Y
Xue
D000293:Adolescent; D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000473:pathology; D003561:Cytarabine / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006248:Harringtonines / Q000494:pharmacology; D000077863:Homoharringtonine; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008875:Middle Aged; D008942:Mitoxantrone / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D006248:Harringtonines; D003561:Cytarabine; D000077863:Homoharringtonine; D008942:Mitoxantrone; D003630:Daunorubicin