[Use of polymerase chain reaction for pediatric diagnosis].
Basic principles of main molecular biology techniques: polymerase chain reaction (PCR) and its variants; asymmetric PCR, ARMS, nested PCR, multiplex PCR and competitive PCR are discussed. Nucleic acids techniques are making increasing progress in molecular diagnostics. Sequence analysis of amplified DNA allows better identification of the pathogen, detection of mutant genes and more accurate prognosis and therapy of certain diseases.
Zakład Genetyki Medycznej AM w Lublinie.
M
M
Kostuch
A
A
Stenzel
J
J
Wojcierowski
D002648:Child; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D010372:Pediatrics / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*