EDTA-induced changes in platelet structure and function: adhesion and spreading.
Ethylenediamine tetraacetic acid (EDTA) is an effective anticoagulant, but unfortunately causes structural, biochemical and functional damage to human platelets. Some of the functional injuries, such as adhesion to and spreading on surfaces, are considered irreversible. The present investigation has evaluated that hypothesis. Our findings indicate that platelets from EDTA platelet-rich plasma (PRP) or CCD PRP to which EDTA has been added do not adhere to glass or plastic surfaces. However, when platelets from EDTA PRP or CCD PRP containing added EDTA are washed and resuspended under conditions reported to cause irreversible dissociation of the fibrinogen receptor, GPIIb/IIIa, then washed and resuspended in buffer containing Ca2+ and Mg2+ ions will adhere and spread in the same manner as platelets not exposed to EDTA. The ability to recover adhesive function may explain why EDTA platelets are able to sustain clot retraction as well as CCD platelets.
Department of Laboratory Medicine, University of Minnesota Medical School, Minneapolis 55455, USA.
J G
JG
White
G
G
Escolar
D000925:Anticoagulants / Q000009:adverse effects / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D002118:Calcium / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003018:Clot Retraction; D004492:Edetic Acid / Q000009:adverse effects / Q000494:pharmacology*; D005898:Glass; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000648:ultrastructure; D008274:Magnesium / Q000494:pharmacology; D008863:Microspheres; D010973:Platelet Adhesiveness / Q000187:drug effects*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D011145:Polyvinyls
D000925:Anticoagulants; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011145:Polyvinyls; D019343:Citric Acid; C013215:polyvinyl acetate; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium