PAL31, a novel nuclear protein, expressed in the developing brain.
We cloned a cDNA encoding a novel protein (PAL31) predominantly expressed in the fetal rat brain by differential display. PAL31 contains leucine-rich repeat domains, a highly acidic region and a putative nuclear localization signal. PAL31 has 50-70% similarity to SSP29, APRIL, LANP, PHAP I, and PP32. Expression of PAL31 mRNA in the brain was high during the fetal period and decreased after birth. Immunohistochemical studies showed that PAL31 is expressed in the entire embryonic brain, whereas in the adult brain its expression is restricted to the subventricular zone where there are neural progenitor cells. It was also revealed that PAL31 is colocalized with PCNA in the nucleus, indicating that the PAL31 expression is developmentally regulated. Considering the primary structure of PAL31 and its spatiotemporal expression pattern, PAL31 is a novel nuclear protein related to the development of the brain through the proliferation of neuronal cells.
Laboratory of Cellular Biochemistry, University of Tokyo, Tokyo, 113-8657, Japan.
H
H
Mutai
Y
Y
Toyoshima
W
W
Sun
N
N
Hattori
S
S
Tanaka
K
K
Shiota
D020816:Amino Acid Motifs; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology* / Q000378:metabolism*; D002452:Cell Count; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005456:Fluorescent Dyes; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D064231:Nestin; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D016716:PC12 Cells; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C412378:Anp32b protein, rat; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D007381:Intermediate Filament Proteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger