Involvement of cellular caveolae in bacterial entry into mast cells.
Caveolae are subcellular structures implicated in the import and transcytosis of macromolecules and in transmembrane signaling. To date, evidence for the existence of caveolae in hematopoietic cells has been ambiguous. Caveolae were detected in the microvilli and intracellular vesicles of cultured mouse bone marrow-derived mast cells (BMMCs). CD48, a receptor for FimH-expressing (type 1 fimbriated) Escherichia coli, was specifically localized to plasmalemmal caveolae in BMMCs. The involvement of caveolae in bacterial entry into BMMCs was indicated because caveolae-disrupting and -usurping agents specifically blocked E. coli entry, and markers of caveolae were actively recruited to sites of bacterial entry. The formation of bacteria-encapsulating caveolar chambers in BMMCs represents a distinct mechanism of microbial entry into phagocytes.
Departments of Pathology and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
J S
JS
Shin
Z
Z
Gao
S N
SN
Abraham
D018829:Adhesins, Bacterial / Q000378:metabolism*; D018830:Adhesins, Escherichia coli; D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism*; D001422:Bacterial Adhesion; D000071178:CD48 Antigen; D051242:Caveolin 1; D022461:Caveolins; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure; D002772:Cholera Toxin / Q000494:pharmacology; D003505:Cyclodextrins / Q000494:pharmacology; D004705:Endocytosis; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D036781:Fimbriae Proteins; D017261:Glycosylphosphatidylinositols / Q000032:analysis; D008407:Mast Cells / Q000378:metabolism* / Q000382:microbiology* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron
D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; D015703:Antigens, CD; D000071178:CD48 Antigen; D051242:Caveolin 1; D022461:Caveolins; C000604066:Cd48 protein, mouse; D003505:Cyclodextrins; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; C058769:fimH protein, E coli; D036781:Fimbriae Proteins; D002772:Cholera Toxin