A Kinetic Study on the Interaction between Tazobactam (A Penicillanic Acid Sulphone Derivative) and Active-Site Serine beta-Lactamases.
The interaction between tazobactam and several chromosome- and plasmid-encoded (TEM, SHV, PSE types) class A and C beta-lactamases was studied by spectrophotometry. Tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner beta-lactam. A detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum beta-lactamases; the presence of a turn-over of acyl-enzyme complex was also evaluated.
Department of Biomedical Sciences and Technologies, University of L'Aquila, Loc. Coppito - 67100, L'Aquila, Italy.
M
Perilli
N
Franceschini
G
Bonfiglio
B
Segatore
S
Stefani
G
Nicoletti
Tavio Perez Md
C
Bianchi
A
Zollo
G
Amicosante