Optical characterization of liposomes by right angle light scattering and turbidity measurement.
Liposomes have frequently been used as models of biomembranes or vehicles for drug delivery. However, the systematic characterization of lipid vesicles by right angle light scattering and turbidity has not been carried out despite the usefulness of such studies for size estimation. In this study, liposomes of various sizes were prepared by sonication and extrusion. The mean cumulant radii of the vesicles were determined by dynamic light scattering. The lamellarities were estimated based on fluorescence quenching of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L-alpha-phosph ati dylethanolamine by sodium dithionite. Right angle light scattering intensity and optical density at 436 nm per unit lipid concentration were measured as a function of vesicle radius. With a vesicle radius < or =100 nm, the optical parameters could be well explained by the Rayleigh-Gans-Debye theory in which the liposomes were modeled as homogeneous spheres with mean refractive indices determined by the volume fractions of lipids in vesicles.
Graduate School of Biostudies and Graduate School of Pharmaceutical Sciences, Kyoto, Japan. katsumim@pharm.kyoto-u.ac.jp
K
K
Matsuzaki
O
O
Murase
K
K
Sugishita
S
S
Yoneyama
K
K
Akada
M
M
Ueha
A
A
Nakamura
S
S
Kobayashi
D004227:Dithionite; D005456:Fluorescent Dyes; D008081:Liposomes / Q000737:chemistry*; D008962:Models, Theoretical; D009391:Nephelometry and Turbidimetry; D010316:Particle Size; D010714:Phosphatidylethanolamines; D012031:Refractometry; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes; D008081:Liposomes; D010714:Phosphatidylethanolamines; D004227:Dithionite; C042077:N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine