Inhibition of the central heme oxygenase-carbon monoxide pathway increases 2-deoxy-D-glucose-induced hypothermia in rats.
The present study was designed to test the hypothesis that carbon monoxide (CO) plays a role in 2-deoxy-D-glucose (2-DG)-induced hypothermia. The body temperature (T(b)) of awake, unrestrained rats was measured before and after systemic administration of 2-DG (50 mg/kg) and intracerebroventricular administration of zinc deuteroporphyrin 2,4-bis glycol (ZnDPBG, a heme-oxygenase inhibitor, 200 nmol/4 microl). We observed a significant reduction in body temperature after 2-DG injection. ZnDPBG alone caused no significant change in body temperature. When the two treatments were combined, 2-DG-induced hypothermia was significantly increased. The data indicate that heme oxygenase-carbon monoxide pathway plays a key role in 2-DG-induced hypothermia, inhibiting 2-DG-induced hypothermia.
Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, 14040-904 Ribeirão Preto, São Paulo, Brazil.
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D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D002248:Carbon Monoxide / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D003847:Deoxyglucose / Q000008:administration & dosage*; D003905:Deuteroporphyrins / Q000494:pharmacology; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D007036:Hypothermia, Induced; D007276:Injections, Intraventricular; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D003905:Deuteroporphyrins; D004791:Enzyme Inhibitors; C060750:zinc deuteroporphyrin IX 2,4-bis(glycol); D002248:Carbon Monoxide; D003847:Deoxyglucose; D006419:Heme Oxygenase (Decyclizing)