In vitro antibiotic susceptibility of Francisella tularensis isolated from humans and animals.
The in vitro susceptibility of 38 strains of Francisella tularensis (biovar F. tularensis palaearctica) was determined using Etests on cysteine heart agar plates with 2% haemoglobin. All strains were susceptible to the antibiotics traditionally used to treat tularaemia, such as streptomycin (MIC(90) 4.0 mg/L), tetracycline (MIC(90) 0.38 mg/L) and chloramphenicol (MIC(90) 0.38 mg/L), and to aminoglycosides, such as tobramycin (MIC(90) 1.5 mg/L) and gentamicin (MIC(90) 1.0 mg/L). The quinolones examined had low MIC(90)s: ciprofloxacin, 0.016 mg/L; levofloxacin, 0.016 mg/L; grepafloxacin, 0.047 mg/L; and trovafloxacin, 0.032 mg/L. In contrast, all the strains were resistant to beta-lactams and azithromycin. Quinolones thus seem to be promising drugs for the treatment of tularaemia.
Clinical Microbiology Laboratory and Department of Infection Control, Oulu University Hospital, Oulu, Finland. irma.ikaheimo@ppshp.fi
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I
Ikäheimo
H
H
Syrjälä
J
J
Karhukorpi
R
R
Schildt
M
M
Koskela
D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003411:Arvicolinae; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005604:Francisella tularensis / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014406:Tularemia / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial