[Chromosome marker--2q- during carcinogenesis and development of human lung cancer].
OBJECTIVE
To study the cytogengtic alterations during the development of human lung cancer and to clarify the genetic basis of lung cancer.


METHODS
Using Fluorescence in situ Hybridization FISH, we have studied the chromosomal alterations of chromosome 2 and 3 in human bronchial epithelial cell line M, and 12 primary non-small cell lung cancer (NSCLC) specimens.


RESULTS
2q- was found in the later passage of human bronchial epithelial cell line M, and 3 out of 7 primary NSCLC specimens tested. 3p- being noted in five cases of 12 primary NSCLC patients examined was the first cytogenetic discovery and the most prominent abnormality in lung cancer. 2q- was rarely reported in human lung cancer before. But loss of heterozygosity by RFLP analysis for 2q had been reported in lung cancer.


CONCLUSIONS
2q- is also a common chromosome abnormality in human lung cancer. There would also be one or more putative tumor suppressor gene(s) on the long arm of chromosome 2. Loss of the gene(s) may contribute to the carcinogenesis of human non-small-cell lung cancer.
Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Y
Y
Lü
X
X
Dong
S
S
Guo
D000230:Adenocarcinoma / Q000235:genetics; D000328:Adult; D000368:Aged; D001980:Bronchi / Q000166:cytology; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002478:Cells, Cultured; D018914:Cells, Immobilized; D002872:Chromosome Deletion; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged
D005819:Genetic Markers