Chromosomes 4 and 13 in beta-carboline-induced seizures in mice: benzodiazepine binding.
Methyl beta-carboline-3-carboxylate (beta-CCM) is a ligand for the benzodiazepine (BZD) binding site of the GABA-A receptors with convulsive properties. We provided evidence for the involvement of a fragment of mouse chromosomes 4 and 13 in beta-CCM-induced seizures in a previous paper. Here, we analyzed, through [3H]-flumazenil binding, whether central BZD binding sites could be involved in the physiological processes underlying these differences of genetic sensitivities. In the JE/Le strain, where the effects of the chromosome 4 fragment can be analyzed, we found associations between [3H]-flumazenil binding and the convulsive action of beta-CCM. On the contrary, this no longer holds true in C3XtEso strain, where the effects of the chromosome 13 fragment were observed.
Génétique, Neurogénétique et Comportement, UPR 9074 CNRS, Orléans, France.
Y
Y
Clément
B
B
Martin
D
D
Bondoux
P
P
Venault
J M
JM
Launay
G
G
Chapouthier
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002243:Carbolines / Q000494:pharmacology*; D002874:Chromosome Mapping; D003292:Convulsants / Q000494:pharmacology*; D005442:Flumazenil / Q000493:pharmacokinetics; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D011869:Radioligand Assay; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology*; D012640:Seizures / Q000139:chemically induced / Q000235:genetics* / Q000503:physiopathology; D013045:Species Specificity; D014316:Tritium
D002243:Carbolines; D003292:Convulsants; D011963:Receptors, GABA-A; D014316:Tritium; D005442:Flumazenil; C036150:beta-carboline-3-carboxylic acid methyl ester