An inward rectifier and a voltage-dependent K+ current in single, cultured pericytes from bovine heart.
OBJECTIVE
The purpose of this study was to describe passive electrical properties and major membrane currents in coronary pericytes.


METHODS
78 single, cultured bovine pericytes were studied with the patch-clamp technique in the whole-cell mode.


RESULTS
The membrane potential of the cells was -48.9+/-9.6 mV (mean+/-S.D.) with 5 mM and -23.2+/-2.2 mV with 60 mM extracellular K+. The membrane capacitance was 150.2+/-123.2 pF. The current-voltage relation of the pericytes was dominated by an inward current at hyperpolarized potentials and an outward current at depolarized potentials. Increasing extracellular K+ from 5 to 60 mM led to an increase of the inward current and to a shift of this current to more depolarized potentials. The inward current was very sensitive to extracellular barium (50 microM). The maximum slope conductance of the cells at hyperpolarized potentials was 2.9+/-2.8 nS. Inward rectification of whole-cell currents was steep (slope factor = 6.8 mV). With elevated external K+ the outward current reversed near the potassium equilibrium potential. Onset of the outward current was sigmoid and inactivation of this current was monoexponential, slow (time constant = 12.8 s) and incomplete. Voltage-dependence of outward current steady-state activation was steep (slope factor = 4.6 mV). The outward current was very sensitive to 4-aminopyridine (dissociation constant = 0.1 mM). The maximum slope conductance at depolarized potentials was 16.6+/-15.6 nS.


CONCLUSIONS
We report for the first time, patch-clamp recordings from coronary pericytes. An inward rectifier and a voltage-dependent K+ current were identified and characterized. Regulation of these currents may influence coronary blood flow.
von Beckerath
N
N
Deutsches Herzzentrum and 1.Medizinische Klinik, Technische Universität München, Munich, Germany. beckerath@dhm.mhn.de
Nees
S
S
Neumann
F J
FJ
Krebs
B
B
Juchem
G
G
Schömig
A
A
D000818:Animals; D002196:Capillaries; D002417:Cattle; D002478:Cells, Cultured; D003326:Coronary Circulation; D004730:Endothelium, Vascular / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques; D020286:Pericytes / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology*; D018709:Statistics, Nonparametric; D014699:Venules
D015221:Potassium Channels