Early intervention in post-transplant lymphoproliferative disorders based on Epstein-Barr viral load.
Using a real-time quantitative PCR assay, we identified two patients with EBV-related lymphoproliferative disorders at a very early stage. Both had received an unmanipulated bone marrow transplant with anti-thymocyte globulin for conditioning. To estimate virus-specific immunity, the frequencies of EBV-specific CD8+ T cells were measured using an enzyme-linked immunospot assay. The frequencies of EBV-specific CD8+ T cells of the two were 3.2 and 7.7%, respectively, which had possibly expanded in vivo. After withdrawing the immunosuppressive agents or administering donor lymphocytes transfusion, their symptoms regressed in parallel with the viral load.
Hoshino
Y
Y
Department of Pediatrics, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Kimura
H
H
Kuzushima
K
K
Tsurumi
T
T
Nemoto
K
K
Kikuta
A
A
Nishiyama
Y
Y
Kojima
S
S
Matsuyama
T
T
Morishima
T
T
D000328:Adult; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002648:Child; D004279:DNA, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000139:chemically induced / Q000150:complications / Q000175:diagnosis; D005260:Female; D004854:Herpesvirus 4, Human / Q000254:growth & development*; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000175:diagnosis* / Q000821:virology; D019562:Viral Load / Q000009:adverse effects*
D004279:DNA, Viral