Effects of cholinergic depletion on neural activity in different laminae of the rat barrel cortex.
The purpose of these experiments was to determine the effects of cholinergic depletion on spontaneous and evoked activity of neurons in the different layers of the posteromedial barrel subfield (PMBSF) of the rat somatosensory cortex. Acetylcholine neurons in nucleus basalis of Meynert (NBM) were selectively lesioned with an immunotoxin (IT), 192 IgG-saporin. Spontaneous activity was significantly lower in layers II-III, Va, and VI in IT-injected animals compared to control animals. Evoked activity was significantly lower in layers II-III, IV, Vb, and VI of IT-injected animals compared to control animals. The largest difference was observed in layer Vb. Thus, cholinergic depletion causes significant changes in the magnitude of spontaneous and evoked activity but these differences are not completely in register with one another.
Departments of Anatomy and Neurobiology, College of Medicine, The University of Tennessee, Memphis, 855 Monroe Avenue, Memphis, TN 38163, USA. pherron@utmem.edu
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Schweitzer
D000109:Acetylcholine / Q000172:deficiency*; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D020532:Basal Nucleus of Meynert / Q000166:cytology / Q000187:drug effects*; D018678:Cholinergic Agents / Q000008:administration & dosage*; D005071:Evoked Potentials / Q000187:drug effects; D000922:Immunotoxins / Q000008:administration & dosage; D007276:Injections, Intraventricular; D008297:Male; D008845:Microinjections; D009699:N-Glycosyl Hydrolases; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013003:Somatosensory Cortex / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014738:Vibrissae / Q000294:innervation / Q000502:physiology
C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D018678:Cholinergic Agents; D000922:Immunotoxins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins; D000109:Acetylcholine