Increased airway responsiveness after skin sensitisation to 3-carene, studied in isolated guinea pig lungs.
Inhalation of 3-carene has been shown to induce bronchoconstriction in concentrations not far from the threshold limit value. In this study, one group of guinea-pigs were sensitised by dermal exposure to 3-carene according to the modified Cumulative Contact Enhancement Test protocol and another group of animals was used as controls. Lungs from the skin-sensitised and control guinea-pigs were perfused with diluted autologous blood (13 ml blood/87 ml buffer) and exposed to 3-carene at an air concentration of 3000 mg/m(3). In both groups there was a reduction in compliance and conductance but this reduction was significantly (P<0.05) more pronounced (2.5-3 times) in lungs obtained from sensitised animals than from control animals. In a previous study with similar design, but with plain buffer instead of diluted autologous blood as perfusate, we found no statistically significant difference in lung bronchoconstriction. Thus, it is concluded that skin sensitisation can increase lung reactivity to 3-carene and that important mediators of this effect seem to be present in the blood.
Division of Inhalation Toxicology, Institute of Environmental Medicine, Karolinska Institute, PO Box 210, 171 77, Stockholm, Sweden. lena.lastbom@imm.ki.se
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Låstbom
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Boman
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Camner
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Ryrfeldt
D000280:Administration, Inhalation; D000287:Administration, Topical; D000485:Allergens / Q000276:immunology*; D000818:Animals; D000081008:Bicyclic Monoterpenes; D016535:Bronchial Hyperreactivity / Q000097:blood / Q000139:chemically induced / Q000276:immunology*; D001985:Bronchial Provocation Tests; D004342:Drug Hypersensitivity / Q000097:blood / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D007114:Immunization; D008168:Lung / Q000187:drug effects / Q000276:immunology*; D008170:Lung Compliance / Q000187:drug effects / Q000276:immunology; D039821:Monoterpenes; D010477:Perfusion; D012867:Skin / Q000276:immunology*; D013729:Terpenes / Q000276:immunology*
D000485:Allergens; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D013729:Terpenes; C030218:3-carene