[The application of fluorescence in situ hybridization performed on the decolorized G-banding chromosomes in detecting the marker chromosomes of gastric cancer].
OBJECTIVE
Using a rapid, accurate method that detects the marker chromosomes of gastric cancer and enhancing the ability of discriminating complicated chromosome rearrangements of gastric cancer.


METHODS
The improved method of fluorescence in situ hybridization(FISH) performed on the decolorized G-banding chromosome was used.


RESULTS
The changes of two marker chromosomes (M1, M2) of the cell line(SGC-7901) of gastric cancer and one marker chromosomes(M3) of one primary gastric cancer were respectively analyzed by this method. The M1, M2 and M3 had complicated structural chromosome aberrations: del(7)(p15)/del(7)(q22), t(1;3)(p11;q11) and del(7)(q32).


CONCLUSIONS
This method showed strong signals, low backgrounds and well-repetitions. It may play an important part in exploring the chromosome rearrangements in the process of pathogenesis and development of gastric cancer.
Department of Pathology, Cancer Center, Sun Yat-sen University of Medical Science, Guangzhou, Guangdong, P. R. China. xiajc51@hotmail.com
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Xia
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Zhang
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Guan
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Wang
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Liu
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Zhang
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Li
D002869:Chromosome Aberrations; D002871:Chromosome Banding; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D013274:Stomach Neoplasms / Q000235:genetics*
D005819:Genetic Markers