Farnesol-induced cell death and stimulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity in tobacco cv bright yellow-2 cells.
Growth inhibition of tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells by mevinolin, a specific inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) could be partially overcome by the addition of farnesol. However, farnesol alone inhibited cell division and growth as measured by determination of fresh weight increase. When 7-d-old tobacco cv Bright Yellow-2 cells were diluted 40-fold into fresh culture, the cells exhibited a dose-dependent sensitivity to farnesol, with 25 microM sufficient to cause 100% cell death, as measured by different staining techniques, cytometry, and monitoring of fragmentation of genomic DNA. Cells were less sensitive to the effects of farnesol when diluted only 4-fold. Farnesol was absorbed by the cells, as examined by [1-(3)H]farnesol uptake, with a greater relative enrichment by the more diluted cells. Both mevinolin and farnesol treatments stimulated apparent HMGR activity. The stimulation by farnesol was also reflected in corresponding changes in the steady-state levels of HMGR mRNA and enzyme protein with respect to HMGR gene expression and enzyme protein accumulation.
Centre National de la Recherche Scientifique, Institut de Biologie Moléculaire des Plantes, Département Isoprénoïdes, Institut de Botanique, Université Louis Pasteur, 28 rue Goethe, F-67083 Strasbourg, France.
A
A
Hemmerlin
T J
TJ
Bach
D015152:Blotting, Northern; D015153:Blotting, Western; D016923:Cell Death; D002455:Cell Division; D048429:Cell Size; D053938:DNA Fragmentation; D004591:Electrophoresis, Polyacrylamide Gel; D005204:Farnesol / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology; D008148:Lovastatin / Q000494:pharmacology; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000032:analysis; D014026:Nicotiana / Q000166:cytology / Q000201:enzymology / Q000502:physiology*
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D012333:RNA, Messenger; D005204:Farnesol; D008148:Lovastatin; D006903:Hydroxymethylglutaryl CoA Reductases