Cyclic expression of class I alcohol dehydrogenase in male rats treated with ethanol.
Continuous infusion of ethanol-containing diets has been demonstrated to generate well-defined pulses in blood and urine ethanol concentrations that occur with a frequency of approximately 6 days. The present study aimed to determine if hepatic class I alcohol dehydrogenase was the cause of these cycles. Adult male rats were fed an ethanol-containing diet by continuous intragastric infusion. Hepatic ADH activity, class I ADH mRNA level and rate of class I ADH gene transcription fluctuated in a cyclic pattern that positively correlated with UECs, and inhibition of ADH with 4-methylpyrazole abolished the UEC pulses. These data demonstrate for the first time an ethanol-dependent regulation of rat hepatic class I ADH. The cyclic behavior of the ethanol levels correlates with changes in class I ADH expression and implies adaptability of the ethanol eliminating system to high concentrations of alcohol.
Department of Pediatrics, University of Arkansas for Medical Science, Little Rock, Arkansas, USA.
T M
TM
Badger
J O
JO
Hoog
S
S
Svensson
R E
RE
McGehee
C
C
Fang
M J
MJ
Ronis
M
M
Ingelman-Sundberg
D000426:Alcohol Dehydrogenase / Q000096:biosynthesis*; D000818:Animals; D002492:Central Nervous System Depressants / Q000008:administration & dosage* / Q000097:blood / Q000652:urine; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood / Q000652:urine; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008099:Liver / Q000201:enzymology*; D008297:Male; D010507:Periodicity; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D002492:Central Nervous System Depressants; D012333:RNA, Messenger; D000431:Ethanol; D000426:Alcohol Dehydrogenase