Enzymatic degradation of nitriles by a Candida guilliermondii UFMG-Y65.
Candida guilliermondii UFMG-Y65, isolated from a gold mine, was able to utilize different nitriles and the corresponding amides as sole source of nitrogen, at concentrations up to 2 M. Resting cells cultivated on YCB-acetonitrile medium showed nitrile hydrolyzing enzyme activities against acrylonitrile and benzonitrile. These enzymes were inducible and intracellular; the optimum pH was 7.0-8.0, and the optimum temperature 25 degrees C-30 degrees C. Liquid chromatographic analysis indicated that C. guilliermondii UFMG-Y65 metabolized 12 mM benzonitrile to 11 mM benzoic acid and 10 mM acrylonitrile to 7.9 mM acrylic acid. The results suggest that C. guilliermondii UFMG-Y65 may be useful for the bioproduction of amides and acids, and for the bioremediation of environments contaminated with nitriles.
Dias
J C
JC
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Rezende
R P
RP
Rosa
C A
CA
Lachance
M A
MA
Linardi
V R
VR
D000097:Acetonitriles / Q000378:metabolism; D000181:Acrylonitrile / Q000378:metabolism; D000577:Amides / Q000378:metabolism; D001673:Biodegradation, Environmental; D002175:Candida / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D006046:Gold; D006863:Hydrogen-Ion Concentration; D008906:Mining; D009570:Nitriles / Q000378:metabolism*; D013696:Temperature
D000097:Acetonitriles; D000577:Amides; D004785:Environmental Pollutants; D009570:Nitriles; D006046:Gold; C014356:benzonitrile; D000181:Acrylonitrile; C032159:acetonitrile