Crystallization and preliminary X-ray crystallographic analysis of Escherichia coli CyaY, a structural homologue of human frataxin.
CyaY is a 106-residue protein from Escherichia coli. It shows amino-acid sequence similarity to human frataxin and a frataxin homologue in Saccharomyces cerevisiae, Yfh1p. The former is associated with the disease Friedreich ataxia and the latter plays a key role in iron homeostasis in mitochondria. CyaY has been overexpressed in soluble form in E. coli. The recombinant protein with a His(6) tag at its C-terminus has been crystallized at 296 K using polyethylene glycol (PEG) 4000 as a precipitant. Native diffraction data have been collected to 1.8 A using Cu Kalpha X-rays. The crystals belong to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell parameters a = b = 44.66, c = 99.87 A, alpha = beta = 90.0, gamma = 120.0 degrees. The asymmetric unit contains one molecule of recombinant CyaY, with a corresponding V(m) of 2.13 A(3) Da(-1) and solvent content of 42.3%.
Department of Chemistry, College of Natural Sciences, Division of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.
M G
MG
Lee
S J
SJ
Cho
J K
JK
Yang
H K
HK
Song
S W
SW
Suh
D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D006801:Humans; D033862:Iron-Binding Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D000096923:Frataxin
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D033862:Iron-Binding Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D011994:Recombinant Proteins; D000096923:Frataxin; C120906:CyaY protein, E coli