[Study on expression of IRF-1 gene in human leukemia with RT-PCR and the silver sequence techniques].
OBJECTIVE
To explore preliminarily the changes of IRF-1 gene expression in leukemias.


METHODS
The expression of IRF-1 gene in 24 leukemia patients and 3 leukemic cell lines were studied with RT-PCR and the silver sequence techniques.


RESULTS
No expression of IRF-1 gene in 3 patients, and significantly decreased expressions in 5 patients and in HL-60 and HIMeg cell lines were revealed.


CONCLUSIONS
The mechanism of human leukemogenesis may be partly associated with no or low-expression of IRF-1 gene.
Changhai Hospital, Second Military Medical University.
F
F
Xia
Y
Y
Yuan
W
W
Zhang
D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D050856:Interferon Regulatory Factor-1; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D016622:Silver Staining; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D004268:DNA-Binding Proteins; C494257:IRF1 protein, human; D050856:Interferon Regulatory Factor-1; D010750:Phosphoproteins; D014157:Transcription Factors