Galectin-3 is upregulated in microglial cells in response to ischemic brain lesions, but not to facial nerve axotomy.
We have recently demonstrated that the beta-galactoside-specific lectin galectin-3 is expressed by microglial cells in vitro, but not by normal resting microglia in vivo. In the present study, we have analyzed the expression of galectin-3 by microglia under traumatic conditions in vivo using two experimental rat models which substantially differ in the severity of lesion related to a breakdown of the blood-brain barrier (BBB) and the occurrence of inflammatory processes. These two features are absent after peripheral nerve lesion and present after cerebral ischemia. Here we show that, following facial nerve axotomy under conditions allowing (nerve anastomosis) or not subsequent regeneration (nerve resection), galectin-3 is not expressed by microglia in the corresponding facial nucleus 1-112 days after lesion. Galectin-3 is also absent in microglia at sites of a defective BBB in the normal brain, such as the circumventricular organs. Following experimental ischemia (i.e., permanent occlusion of the middle cerebral artery), in contrast, galectin-3 becomes strongly expressed by activated microglia as early as 48 hours after trauma, as determined by immunohistochemistry and Western blot analysis. Our findings suggest that the expression of galectin-3 by microglia in vivo correlates with the state of microglial activation.
Institute I of Anatomy, University of Cologne, Cologne, Germany.
M
M
Walther
S
S
Kuklinski
P
P
Pesheva
O
O
Guntinas-Lichius
D N
DN
Angelov
W F
WF
Neiss
H
H
Asou
R
R
Probstmeier
D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism*; D019771:Axotomy; D002545:Brain Ischemia / Q000378:metabolism*; D020220:Facial Nerve Injuries / Q000378:metabolism*; D005260:Female; D037502:Galectin 3; D008264:Macrophages / Q000378:metabolism; D017628:Microglia / Q000378:metabolism*; D051381:Rats; D020318:Rats, Long-Evans; D017208:Rats, Wistar; D015854:Up-Regulation
D000943:Antigens, Differentiation; D037502:Galectin 3