Cloning and sequence analysis of cDNA coding for a lectin from Helianthus tuberosus callus and its jasmonate-induced expression.
Two lectins (designated as HTA I and HTA II) that seemed to be isolectins were found in Helianthus tuberosus callus. cDNA encoding HTA I was isolated from a ZAP Express expression library by immunoselection by using the anti-HTA antiserum. The sequence of this cDNA consisted of 432 bp nucleotides coding for a polypeptide of 143 amino acid residues (Mr, 15,314). When introduced into E. coli, the cDNA directed the synthesis of active HTA I as indicated by the hemagglutination activity. The deduced amino acid sequence showed homology with some lectins and jasmonate-induced proteins. When callus was cultured in the presence of methyl jasmonate (MeJA), the hemagglutination activity increased in a dose-dependent manner. The levels of expression of the HTA protein and of the corresponding mRNA also increased in the treated callus. In view of these results, HTA I is considered to be a jasmonate-induced protein.
Hokkaido Food Processing Research Center, Ebetsu, Japan. nakagawa@foodhokkaido.gr.jp
R
R
Nakagawa
D
D
Yasokawa
Y
Y
Okumura
K
K
Nagashima
D000085:Acetates / Q000494:pharmacology; D000373:Agglutinins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant; D006368:Helianthus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology; D037121:Plant Lectins; D021122:Protein Subunits; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D000085:Acetates; D000373:Agglutinins; D003517:Cyclopentanes; D017931:DNA Primers; D018076:DNA, Complementary; D018744:DNA, Plant; C117435:Helianthus tuberosus lectin; D037102:Lectins; D054883:Oxylipins; D010937:Plant Growth Regulators; D037121:Plant Lectins; D021122:Protein Subunits; D012333:RNA, Messenger; D018749:RNA, Plant; C043696:jacalin; C072239:methyl jasmonate