Mice lacking in gp91 phox subunit of NAD(P)H oxidase showed glomus cell [Ca(2+)](i) and respiratory responses to hypoxia.
The hypothesis that NAD(P)H oxidase may serve as an oxygen sensor was tested using the mice deficient (knock-out) in gp91phox subunit of NAD(P)H oxidase enzyme complex and compared with wild-type (C57BL/6J) strain measuring the ventilatory and glomus cell intracellular calcium ([Ca(2+)](i)) responses of carotid body to hypoxia. The hypoxic ventilatory responses as well as the [Ca(2+)](i) were preserved in the NAD(P)H oxidase knock-out mice. NAD(P)H oxidase, though a major source of oxygen radical production, is not the oxygen sensor in mice carotid body.
Roy
A
A
Department of Physiology, University of Pennsylvania School of Medicine, B-400 Richards Building, 3700 Hamilton Walk, Philadelphia, PA 19104-6085, USA.
Rozanov
C
C
Mokashi
A
A
Daudu
P
P
Al-mehdi
A B
AB
Shams
H
H
Lahiri
S
S
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002344:Carotid Body / Q000166:cytology / Q000378:metabolism*; D006105:Granulomatous Disease, Chronic / Q000235:genetics / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000235:genetics*; D010101:Oxygen Consumption / Q000502:physiology; D010313:Partial Pressure; D012123:Pulmonary Ventilation / Q000502:physiology; D013990:Tidal Volume / Q000502:physiology
D008562:Membrane Glycoproteins; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D002118:Calcium