Infection of dengue 2 virus strains isolated from patients exhibiting different disease severities to human peripheral blood leukocytes and production of cytokines in the infected culture supernatant.
Three strains of type 2 dengue virus (DV-2), which had been isolated from patients exhibiting different disease severity, were inoculated to primary culture of human peripheral blood leukocytes from 3 healthy donors. The percentage of dengue antigen positive cells was highest for the strain isolated from a case of dengue shock syndrome, followed by the strain isolated from a case of dengue hemorrhagic fever, and lowest for the strain isolated from a mild case of dengue fever (DF). Generally, similar trend was observed for the amount of some cytokines released into infected culture supernatant, such as interleukin 6, and tumor necrosis factor-alpha. However, such a trend was not observed for interleukin 1 beta.
Soemanto
B E
BE
Department of Microbiology, Medical Faculty, University of Indonesia, Jakarta.
Hasebe
F
F
Igarashi
A
A
D000818:Animals; D015415:Biomarkers; D002478:Cells, Cultured; D009033:Culicidae; D016207:Cytokines / Q000378:metabolism*; D003716:Dengue Virus / Q000145:classification / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D005260:Female; D006801:Humans; D016131:Lymphocyte Subsets / Q000821:virology*; D008297:Male; D019595:Severe Dengue / Q000821:virology*; D012720:Severity of Illness Index; D014776:Virus Cultivation
D015415:Biomarkers; D016207:Cytokines