The role of mitochondrial dysfunction in regulation of store-operated calcium channels in glioma C6 and human fibroblast cells.
The store-operated calcium influx into electrically non-excitable cells is greatly modified under the condition of deenergized mitochondria in situ. The rate of calcium influx into cells with empty intracellular calcium stores is greatly diminished when cells were pretreated with 2 microM carbonyl cyanide m-chlorophenylhydrazone (a mitochondrial uncoupler) or with 4 microM myxothiazol (an inhibitor of the respiratory chain). We demonstrate that this general phenomenon takes place in the case of transformed (glioma C6 and Ehrlich ascites tumor cells) as well as non-transformed (human fibroblasts) cells. We also demonstrate that the deenergization of mitochondria affects the cellular calcium influx rate and not the calcium pump on the plasma membrane.
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur Street, 02 093, Warsaw, Poland.
M
M
Waśniewska
J
J
Duszyński
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D015220:Calcium Channels / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D019069:Cell Respiration / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007700:Kinetics; D008689:Methacrylates; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014475:Uncoupling Agents / Q000494:pharmacology
D015220:Calcium Channels; D008689:Methacrylates; D013844:Thiazoles; D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; C030517:myxothiazol; D000252:Calcium-Transporting ATPases; D002118:Calcium