Detection of significant bacteriuria by automated urinalysis using flow cytometry.
A new flow cytometry-based automated urine analyzer, the UF-50, was evaluated for its ability to screen urine samples for significant bacteriuria. One hundred eighty-six urine specimens from patients attending an outpatient clinic of a university-based hospital were examined. The results obtained with the UF-50 were compared with those obtained by conventional quantitative urine culture. The UF-50 detected significant bacteriuria with a sensitivity of 83.1%, a specificity of 76.4%, a positive predictive value of 62.0%, a negative predictive value of 90.7%, and an accuracy of 78.5%. These results are comparable to those obtained by previously reported screening procedures. Besides detecting significant bacteriuria, the UF-50 can also perform routine urinalysis, including measurement of concentrations of red blood cells, white blood cells, epithelial cells, and casts, within 70 s. This capability renders this new flow cytometry-based urine analyzer superior to previously reported rapid screening methods.
Department of Urology, Kobe University School of Medicine, Kobe, Japan. okada@med.kobe-u.ac.jp
H
H
Okada
Y
Y
Sakai
S
S
Miyazaki
S
S
Arakawa
Y
Y
Hamaguchi
S
S
Kamidono
D001419:Bacteria / Q000302:isolation & purification; D001431:Bacteriological Techniques; D001437:Bacteriuria / Q000175:diagnosis*; D015169:Colony Count, Microbial; D003470:Culture Media; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D016482:Urinalysis / Q000295:instrumentation* / Q000379:methods; D014556:Urine / Q000382:microbiology
D003470:Culture Media