The expression of presenilin 1 mRNA in skin fibroblasts and brains from sporadic Alzheimer's disease.
We examined the expression of presenilin 1 (PS-1) mRNA in cultured skin fibroblasts taken from living patients with Alzheimer's disease (AD) and human brains taken postmortem from AD patients by RT-PCR analysis. The donors of fibroblasts consisted of 28 cases with AD and 19 neurological patient without dementia (CTL). The brains came from 17 cases with AD and 23 cases with CTL. We found that PS-1 mRNA levels in skin fibroblasts of AD patients were significantly higher than those of CTL patients (p < 0.0001). Moreover, we found that PS-1 mRNA levels in human brains with AD were significantly higher than in those with CTL (p < 0.0001). These findings suggest that high levels of PS-1 mRNA in AD may play an important role in developing AD and that the examination of PS-1 mRNA in skin fibroblasts may be helpful for the diagnosis of AD.
Division of Neurology, Institute of Neurological Sciences, Faculty of Medicine, Tottori University, Yonago, Japan.
K
K
Ikeda
K
K
Urakami
H
H
Arai
K
K
Wada
Y
Y
Wakutani
Y
Y
Ji
Y
Y
Adachi
A
A
Okada
H
H
Kowa
H
H
Sasaki
K
K
Ohno
Y
Y
Ohtsuka
Y
Y
Ishikawa
K
K
Nakashima
D000368:Aged; D000375:Aging / Q000378:metabolism; D000544:Alzheimer Disease / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology*; D005260:Female; D005347:Fibroblasts; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D053764:Presenilin-1; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D012333:RNA, Messenger