Acetone, butanol and ethanol production from domestic organic waste by solventogenic clostridia.
Domestic organic waste (DOW) was washed and dried to 85 % dryness by VAM (The Netherlands). This material contained 25.1 g glucose, 8.4 g xylose and 5.8 g other monosaccharides/100 g dry matter. Using Mansonite steam explosion and enzymatic hydrolysis, a hydrolysate containing 15.4 g glucose, 2.2 g xylose and 0.8 g other monosaccharides per l was made. Clostridium acetobutylicum DSM 1731 produced 1.5 and C. beijerinckii B-592 0.9 g/l ABE and Clostridium LMD 84.48 1.9 g/l IBE, respectively, from this hydrolysate without further supplementation. Incubation with 2 fold concentrated hydrolysate completely impaired ABE production. After removal of unspecific inhibiting components, the yield of ABE production by Clostridium acetobutylicum DSM 1731 increased about 3 fold as compared to the nontreated hydrolysate. From 4 fold concentrated, partially purified, hydrolysate containing 34.2 g glucose/l, ABE production was 9.3 g/l after 120 h as compared to 3.2 g ABE/I from non-concentrated hydrolysate which contained 12.0 g glucose/l after elution over the same column. The concentration of butyric acid in the fermented hydrolysates was 2.2 and 0.4 g/l, respectively. This reasonably low amount of butyric acid showed that the fermentation had proceeded quite well.
Claassen
P A
PA
ATO-DLO, Wageningen, The Netherlands. p.a.m.claassen@ato.dlo.nl
Budde
M A
MA
López-Contreras
A M
AM
D000096:Acetone / Q000378:metabolism*; D000440:Butanols / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D003013:Clostridium / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D009005:Monosaccharides / Q000378:metabolism; D018505:Waste Management / Q000379:methods*; D014866:Waste Products / Q000032:analysis
D000440:Butanols; D009005:Monosaccharides; D014866:Waste Products; D000096:Acetone; D000431:Ethanol; D002482:Cellulose