Serum inhibin B levels in community-dwelling elderly men.
OBJECTIVE
Ageing in men is accompanied by a decline of Leydig cell function, with a 50% decrease of the population means for serum free testosterone between age 25 and 75 years. Information on Sertoli cell function and spermatogenesis in the elderly is scarce. Studies on seminal parameters in ageing men have suggested that spermatogenesis may be fairly well maintained in the elderly, but they included mostly selected subjects and only few men over 60 years. More systematic studies are lacking. The aim of the present study was to assess serum inhibin B levels in elderly men as an index of global Sertoli cell function and spermatogenic activity.


METHODS
Specific immunoassays were used to determine serum levels of inhibin B, gonadotrophins, testosterone and oestradiol in blood obtained between 0800 and 1000 h. from 189 ambulatory, community-dwelling elderly men (age: 70-85 years) and, for comparison, from 51 middle-aged (35-54 years) and 50 young (< 35 years) controls.


RESULTS
All age groups combined, serum inhibin B was only weakly negatively correlated to age (Spearman correlation coefficient: - 0.17; P < 0.01) and more strongly to serum FSH (- 0. 52; P < 0.001). In a multiple regression analysis serum FSH, but not age or serum free testosterone, emerged as an independent determinant of serum inhibin B levels. An age-related decline of median inhibin B levels in the study population was essentially limited to the younger age groups, with stable levels between age 35 and 79 years, and only a modest further decrease thereafter. There was a progressive age-related increase of serum FSH across age groups with, consequently, a marked decrease of the serum inhibin B : FSH ratio. The prevalence of men presenting with low serum inhibin B (below 10th percentile for inhibin B levels in men < 35 years), indicative of deficient Sertoli cell function and spermatogenesis, increased most strikingly between men < 35 years and those 35-54 years, which contrasts with the more progressive increase at an older age of the prevalence of low serum (free) testosterone.


CONCLUSIONS
Global testicular Sertoli cell function and spermatogenic activity, as assessed indirectly through serum inhibin B levels, appear to be well maintained in ambulatory elderly men, albeit there are age-related alterations at the level of the Sertoli cells as indicated by a progressive increase of testicular drive by pituitary FSH.
Departments of Endocrinology and Public Health, Unit for Osteoporosis and Metabolic Bone Diseases, and Laboratories for Andrology and Hormonology, University Hospital of Ghent, Belgium.
A M
AM
Mahmoud
S
S
Goemaere
D
D
De Bacquer
F H
FH
Comhaire
J M
JM
Kaufman
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood / Q000502:physiology*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D019281:Dimerization; D004958:Estradiol / Q000097:blood; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007265:Inhibins / Q000378:metabolism; D016014:Linear Models; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000097:blood*; D029584:Prostatic Secretory Proteins; D020033:Protein Isoforms / Q000097:blood; D012708:Sertoli Cells / Q000502:physiology*; D012738:Sex Hormone-Binding Globulin / Q000032:analysis; D013091:Spermatogenesis / Q000502:physiology*; D018709:Statistics, Nonparametric; D013739:Testosterone / Q000097:blood
D015415:Biomarkers; D010455:Peptides; D029584:Prostatic Secretory Proteins; D020033:Protein Isoforms; D012738:Sex Hormone-Binding Globulin; C043076:beta-microseminoprotein; D013739:Testosterone; D004958:Estradiol; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone