Sulfotransferases in a human colon carcinoma cell line, Caco-2.
We investigated the expression of sulfotransferases (STs) in the human colon carcinoma cell line, Caco-2, which is widely used as a human intestine model for orally administered drugs. By reverse transcription-polymerase chain reaction (RT-PCR), we could detect mRNA expressions of phenol STs (P-ST), hydroxysteroid ST (HS-ST) and estrogen ST (E-ST). Treatment with 1,25-dihydroxyvitamine D3, which induces CYP3A4 expression in Caco-2 cells, did not affect the ST expression of the cells. We detected significant P-ST activity toward 2-naphthol and dopamine in the cell extract. We also observed detectable HS-ST activity toward dehydroepiandrosterone (DHEA), but no E-ST activity toward beta-estradiol was observed in the extract. Analyses by RT-PCR and separation of P-ST activities on ion-exchange column chromatography showed that the thermolabile monoamine phenol sulfotransferase (M-PST) is dominant in Caco-2 cells.
Kyoritsu College of Pharmacy, Tokyo, Japan.
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Satoh
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Matsui
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Tamura
D015239:Arylsulfotransferase / Q000302:isolation & purification; D018938:Caco-2 Cells; D002852:Chromatography, Ion Exchange; D003106:Colon / Q000166:cytology / Q000201:enzymology*; D003110:Colonic Neoplasms; D003637:DEAE-Dextran / Q000737:chemistry; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015238:Sulfotransferases / Q000096:biosynthesis*
D003637:DEAE-Dextran; D015238:Sulfotransferases; D015239:Arylsulfotransferase