Cotranscription of 5S rRNA-tRNA(Arg)(ACG) from Brassica napus chloroplasts and processing of their intergenic spacer.
S1 mapping showed that at least a significant portion of the 5S rRNA and tRNA(Arg)(ACG) is co-transcribed in canola chloroplast, making trnR the last gene transcribed in an operon of which the final sequence is 5'-16S-tRNA(Ile)-tRNA(Ala)-23S-4.5S-5S-tRNA(Arg)-3'. Various RNA termini representing RNA processing sites at several parts of the 5S rRNA-tRNA(Arg) area were detected. This gene spacer is substantially conserved among various species compared here, and a secondary structure model for this chloroplast region in canola applies to other plant sequences. The conservation of this intergenic sequence suggests a functional role, possibly by providing recognition structures for endogenous RNases involved in its maturing process.
Leal-Klevezas
D S
DS
División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia, AP 3838, 44340, Jalisco, Guadalajara, Mexico. DSLeal@academ01.mty.itesm.mx
Martínez-Soriano
J P
JP
Nazar
R N
RN
D001483:Base Sequence; D001937:Brassica / Q000235:genetics*; D002736:Chloroplasts / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D012323:RNA Processing, Post-Transcriptional; D018749:RNA, Plant / Q000235:genetics / Q000502:physiology; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004275:DNA, Ribosomal; D018749:RNA, Plant; D012341:RNA, Ribosomal, 5S; D012347:RNA, Transfer, Arg