The measurement of AgNORs in human glioma cells.
Sixty gliomas obtained by neurosurgical resections were examined. Paraffin blocks were retrieved from pathological files of the Second Affiliated Hospital in Guangzhou Medical College. The methods of argyrophilic technique for AgNORs staining, and Image Analysis System for measurement of AgNORs were used. Six parameters, which included hcount, count, narea, agnrea, agpern and agperc were used to correlated well with histopathological grades (compared grade 2 & 3, grade 3 & 4, and grade 2 & 4, respectively). We concluded that AgNORs is useful in evaluating proliferative activity and assessing the malignancy of human gliomas. It may also be used as a target for anti-neoplastic drugs in the treatment of gliomas.
Hao
C F
CF
Department of Anatomy, Chinese University of Hong Kong, Shatin, NT, Hong Kong.
Wang
Z H
ZH
Yun
J P
JP
Li
H K
HK
Ng
C C
CC
To
K F
KF
Chew-Cheng
S B
SB
Chew
E C
EC
D000293:Adolescent; D000328:Adult; D000368:Aged; D034961:Antigens, Nuclear; D002648:Child; D002675:Child, Preschool; D005260:Female; D005910:Glioma / Q000737:chemistry* / Q000175:diagnosis / Q000473:pathology / Q000556:secondary; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000378:metabolism*; D011379:Prognosis; D016622:Silver Staining / Q000379:methods
D034961:Antigens, Nuclear; D009687:Nuclear Proteins; C051963:nucleolar organizer region associated proteins