Demonstration of a novel glycolytic pathway in the hyperthermophilic archaeon Thermococcus zilligii by (13)C-labeling experiments and nuclear magnetic resonance analysis.
The operation of a novel glycolytic pathway was demonstrated in nongrowing cells of Thermococcus zilligii by analysis of the isotopic enrichment in the end products derived from fermentation of (13)C-labeled glucose. The new pathway involved the formation of formate, derived from C-1 in glucose, via cleavage of a six-carbon carboxylic acid.
Xavier
K B
KB
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal.
da Costa
M S
MS
Santos
H
H
D002247:Carbon Isotopes; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D010732:Phosphofructokinase-1 / Q000378:metabolism; D019712:Thermococcus / Q000254:growth & development / Q000378:metabolism*
D002247:Carbon Isotopes; D006593:Hexokinase; D010732:Phosphofructokinase-1; D005947:Glucose