A case of monoclonal gammopathy associated with acute myelomonocytic leukemia with eosinophilia suggested to be the result of lineage infidelity.
Acute myelomonocytic leukemia (AMMoL) accompanied by monoclonal gammopathy is a rare condition, and its pathogenesis and the cytogenetic mechanism of such leukemogenesis have not been determined in detail. A case of AMMoL with eosinophilia accompanied by immunoglobulin G kappa monoclonal gammopathy is described. Immunophenotypic studies of the peripheral blood and bone marrow mononuclear cells revealed no evidence of abnormally proliferating cells of B-lineage. DNA analyses of bone marrow mononuclear cells containing leukemic cells revealed rearrangement of the kappa-light chain (Igkappa) gene and c-myc and c-jun proto-oncogenes. The intensities of the rearranged bands for these genes on Southern blot analysis suggested the existence of a major population of leukemic cells with rearranged Igkappa gene and minor population(s) of leukemic cells with rearranged c-myc and/or c-jun proto-oncogene(s) in the patient's bone marrow and indicated the occurrence of genetic evolutionary changes in leukemic cells in this patient before starting chemotherapy. These results suggest that these leukemic cells are the most likely candidate for immunoglobulin G kappa monoclonal protein production, and structural abnormalities of c-myc and c-jun proto-oncogenes may have contributed to the evolution of leukemic cells in this patient.
Department of Pediatrics, Nihon University, School of Medicine, Tokyo, Japan. tnagata@med.nihon-u.ac.jp
T
T
Nagata
H
H
Mugishima
A
A
Yoden
K
K
Yoshikawa
T
T
Oguni
K
K
Yamashiro
S
S
Yamamori
K
K
Harada
D015139:Blotting, Southern; D001854:Bone Marrow Cells / Q000737:chemistry; D002455:Cell Division; D002675:Child, Preschool; D004247:DNA / Q000032:analysis; D004802:Eosinophilia / Q000150:complications*; D015321:Gene Rearrangement; D016762:Genes, fos; D016758:Genes, jun; D016259:Genes, myc; D006801:Humans; D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D016130:Immunophenotyping; D015479:Leukemia, Myelomonocytic, Acute / Q000150:complications* / Q000188:drug therapy / Q000235:genetics; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000276:immunology; D008297:Male; D010265:Paraproteinemias / Q000150:complications* / Q000235:genetics; D000090063:Proto-Oncogene Mas
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA