Feasible boundaries of aqueous two-phase systems with NH(3) and CO(2) as recyclable volatile salts.
Aqueous two-phase systems (ATPSs) have great potential for use in the downstream processing of fermentation products. A major drawback of these systems, limiting application in industrial practice up till now, is the consumption of large amounts of auxiliary materials such as polymers and salts. Making use of alternative auxiliaries can diminish this relatively large discharge. A possible approach is to make use of volatile salts induced by combinations of ammonia and carbon dioxide that can be recycled to the extraction system. As part of an ongoing research effort on ATPSs with volatile salts, this work aims at getting more information on the system boundaries or operating conditions of these systems in terms of phase behavior. The results show that the NH(3)/CO(2) ratio is an important parameter that has a large influence on the system boundaries. Both for systems with PEG 2000 and PEG 4000, this ratio has to be larger than about 1.75 to make a liquid-liquid phase separation possible. The most optimal ratio seems to be 2.0 for reasons of solution composition and absence of solid salt.
van Berlo
M
M
Kluyver Laboratory for Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Ottens
M
M
Luyben
K C
KC
van Der Wielen
L A
LA
D000641:Ammonia / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D002219:Carbamates / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000032:analysis / Q000302:isolation & purification; D011108:Polymers; D011312:Pressure; D012492:Salts; D014867:Water / Q000737:chemistry*
D002219:Carbamates; D011108:Polymers; D012492:Salts; D014867:Water; D002245:Carbon Dioxide; D011092:Polyethylene Glycols; D000641:Ammonia; C070766:carbamic acid