Anti-proliferative effect of resveratrol, a natural component of grapes and wine, on human colonic cancer cells.
Resveratrol, a natural polyphenolic phytoalexine present in grapes and wines, has been reported to exert a variety of important pharmacological effects. We investigated the effects of resveratrol on the growth and polyamine metabolism of CaCo-2 human colon cancer cells. Treatment of the CaCo-2 cells with 25 microM resveratrol caused a 70% growth inhibition. The cells accumulated at the S/G2 phase transition of the cell cycle. No signs of cytotoxicity or apoptosis were detected. Resveratrol caused a significant decrease of ornithine decarboxylase (ODC) activity, a key enzyme of polyamine biosynthesis, which is enhanced in cancer growth. ODC inhibition resulted in the reduction of the intracellular putrescine content, indicating that polyamines might represent one of several targets involved in the anti-proliferative effects of resveratrol.
ULP/CJF INSERM 95-09, Laboratory of Metabolic and Nutritional Control in Digestive Oncology, IRCAD, 1 Place de l'Hôpital, 67091, Strasbourg, France.
Y
Y
Schneider
F
F
Vincent
B
B
Duranton
L
L
Badolo
F
F
Gossé
C
C
Bergmann
N
N
Seiler
F
F
Raul
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D018938:Caco-2 Cells; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009955:Ornithine Decarboxylase / Q000378:metabolism; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000378:metabolism; D000077185:Resveratrol; D019685:Rosales / Q000737:chemistry*; D013267:Stilbenes / Q000494:pharmacology*; D014920:Wine / Q000032:analysis*
D000972:Antineoplastic Agents, Phytogenic; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D013267:Stilbenes; D009955:Ornithine Decarboxylase; D000077185:Resveratrol