Absence of delayed chromosomal instability in a normal human fibroblast cell line after 125I iododeoxyuridine.
OBJECTIVE
To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter 125I.


METHODS
Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I125]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis using both conventional Giemsa and fluorescence in situ hybridization (FISH) was undertaken on non-clonal bulk cultures from 2 to 39 days after treatment.


RESULTS
The data show a declining level of unstable aberrations in the progeny of HF19 fibroblasts exposed to [I125]IUdR, eventually reaching control levels.


CONCLUSIONS
The results provide evidence that [125I]IUdR does not induce ongoing chromosomal instability in long-term culture, and gives further support to the use of Auger-electron emitting radionuclides in the treatment and diagnosis of tumours.
Radiation and Genome Stability Unit, MRC, Harwell, Oxon, UK. c.griffin@har.mrc.ac.uk
C S
CS
Griffin
A
A
Neshasateh-Riz
R J
RJ
Mairs
E G
EG
Wright
T E
TE
Wheldon
D002460:Cell Line; D002869:Chromosome Aberrations; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D007065:Idoxuridine / Q000633:toxicity; D017404:In Situ Hybridization, Fluorescence; D007457:Iodine Radioisotopes / Q000633:toxicity*
D007457:Iodine Radioisotopes; D007065:Idoxuridine