[Design of the human tumor-associated VNTR(Muc1) antigen gene, fused with streptavidin, its expression in Escherichia coli. Study of properties of the hybrid protein].
A gene of human tumor-associated antigen VNTR(MUC1) bound to streptavidin, an expression plasmid, and a highly effective hybrid protein-producing strain were constructed. It was shown that the streptavidin leader peptide ensures an effective secretion of the hybrid protein into the periplasmic space of Escherichia coli cells. The hybrid protein was isolated in a homogeneous state and its immunogenic properties were studied.
Gul'ko
L B
LB
State Research Center of Genetics, Russian Federation, Moscow, Russia.
Pavlova
O V
OV
D'iadkov
N A
NA
Okorokova
N A
NA
Ratmanova
K I
KI
Logunova
N N
NN
Bobreneva
R A
RA
Makarov
V A
VA
Iurin
V L
VL
Veĭko
V P
VP
Debabov
V G
VG
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000235:genetics*; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics; D019809:Streptavidin / Q000235:genetics*
D000951:Antigens, Neoplasm; D004274:DNA, Recombinant; D018396:Mucin-1; D011993:Recombinant Fusion Proteins; D019809:Streptavidin