CMV viral load measurements in whole blood and plasma--which is best following renal transplantation?
BACKGROUND
Quantitative commercial assays for cytomegalovirus (CMV) detection have recently been developed. Their role in the management of patients after transplantation needs to be evaluated. Widespread use of these assays will allow for comparison of results between centers and meaningful interpretation of the significance of viral load measurements.


METHODS
Sequential samples from 52 patients after renal transplantation were tested in the murex hybrid capture assay (HCA) and the Roche Amplicor CMV DNA assay (QPCR) and correlated with the development of CMV disease. A comparison of viral loads in plasma and whole blood was also made.


RESULTS
Both assays were sensitive and detected all cases of CMV disease. The specificity and positive predictive value increased from 0.34 and 0.36 to 0.85 and 0.96 for the HCA and 0.37, 0.37 to 0.72 and 0.63 for the QPCR following a receiver operator curve analysis. Higher viral loads were measured using the HCA compared to the QPCR. Response to ganciclovir was associated with a greater than 80% reduction in viral load by HCA or greater than 70% using the QPCR.


CONCLUSIONS
Both assays were highly sensitive. By using a receiver operator curve analysis a cutoff viral load can be determined which maximizes the clinical utility of these assays.
Department of Virology, Barts and the London NHS Trust, West Smithfield, UK.
W
W
Barrett-Muir
J
J
Breuer
C
C
Millar
J
J
Thomas
D
D
Jeffries
M
M
Yaqoob
C
C
Aitken
D000328:Adult; D003587:Cytomegalovirus / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014766:Viremia / Q000821:virology*
D004279:DNA, Viral