Hormonal control on enzymes of osmoregulation in a teleost, Anabas testudineus (BLOCH): an in vivo and in vitro study.
Hormonal control of osmoregulation in teleosts is not well understood. Role of cGH, oGH, PRL, T3 and insulin on gill Na+,K+-ATPase, Mg2+ and Ca2+ ATPases was studied in A. testudineus. Short term administration of cGH, PRL or T3 significantly increased Na+,K+-ATPase, Mg2+ and Ca2+ ATPases, while oGH influenced only Mg2+ ATPase, and insulin stimulated Na+,K+-ATPase. Long-term treatment with cGH and PRL also significantly increased Na+,K+-ATPase activity. GH had an additive with T3 on stimulating Na+,K+-ATPase activity. In vitro addition of cGH and oGH also had definite stimulatory effect on gill Na+,K+-ATPase except for 2ng oGH. Bromocryptine treatment caused a significant reduction on Na+,K+-ATPase activity. Both in vivo and in vitro treatments of cGH and PRL independently reversed the action of bromocryptine on Na+,K+-ATPase. Combined treatment of cGH+PRL was more prominent in stimulating Na+,K+-ATPase in bromocryptine treated fish. Present study reveals that GH, PRL and T3 have definite regulatory role on enzymes of osmoregulation in the teleost Anabas testudineus.
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Department of Zoology, University of Kerala, Trivandrum, India.
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D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000201:enzymology; D013006:Growth Hormone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D066298:In Vitro Techniques; D011388:Prolactin / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology; D014882:Water-Electrolyte Balance / Q000187:drug effects*
D006728:Hormones; D014284:Triiodothyronine; D001971:Bromocriptine; D011388:Prolactin; D013006:Growth Hormone; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase