Identification by differential display of a chicken tolloid-related metalloprotease specifically expressed in the caudal notochord.
While the ventralizing factor Sonic hedgehog is expressed in the entire notochord (Development 121 (1995) 2537) the latter displays distinct ventralizing activities along its rostrocaudal axis. Hence, in HH stage-10 chicken embryo, the caudal notochord exhibits floor plate inducing capacities lost by rostral regions (Development 117 (1993) 205). Therefore, we hypothesize that the caudal notochord produces some cofactors which may contribute to its ventralizing properties. In order to identify such molecules we applied the differential display strategy and isolated a secreted Tolloid-related metalloprotease displaying a regionalized expression in the notochord.
Liaubet
L
L
Centre de Biologie du Développement, UMR-5547 CNRS-Université P. Sabatier, 118 Route de Narbonne, 31062, Cedex 04, Toulouse, France.
Bertrand
N
N
Medevielle
F
F
Pituello
F
F
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D019485:Bone Morphogenetic Proteins; D002642:Chick Embryo; D003001:Cloning, Molecular; D017403:In Situ Hybridization; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D045726:Metalloproteases; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D009672:Notochord / Q000378:metabolism*; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution; D055394:Tolloid-Like Metalloproteinases
D019485:Bone Morphogenetic Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D045726:Metalloproteases; D055394:Tolloid-Like Metalloproteinases; D008666:Metalloendopeptidases