Centrosome reduction during Rhesus spermiogenesis: gamma-tubulin, centrin, and centriole degeneration.
Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate detectable microtubules. The spermatids discard gamma-tubulin in the residual bodies during the spermiation stage. Mature sperm do not have any detectable gamma-tubulin. About half of the centrin associated with the distal centriole degenerates during spermiogenesis and the remainder is intimately bound to the centriolar microtubules. The mature sperm possess highly degenerated distal centrioles. The centriolar microtubules degenerate in the rostral region and the ventral side of the sperm. The study indicates that the centrosome is reduced during rhesus spermiogenesis, but not completely as in mice.
Department of Cell & Developmental Biology, Oregon Regional Primate Research Center/Oregon Health Science University, Beaverton 97006, USA.
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Manandhar
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Schatten
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002502:Centrioles / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D007150:Immunohistochemistry; D008253:Macaca mulatta / Q000502:physiology*; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000648:ultrastructure; D013087:Spermatids / Q000378:metabolism / Q000648:ultrastructure; D013091:Spermatogenesis / Q000502:physiology*; D013094:Spermatozoa / Q000648:ultrastructure; D013737:Testis / Q000502:physiology; D014404:Tubulin / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D014404:Tubulin; C056608:caltractin