[Study on nuclear matrix protein in leukemia cells].
OBJECTIVE
To compare the composition of nuclear matrix proteins (NMP) between leukemia cells and normal bone marrow cells.


METHODS
NMP was isolated by high-salt extration and identified by SDS-PAGE and western blotting.


RESULTS
Increase in NMP was demonstrated in acute and chronic myelogenous leukemic cells as well as in the blast phase of chronic leukemia. On SDS-PAGE, NMPs with molecular weight different from what were seen in normal bone marrow cells were present in both acute and chronic myelogenous leukemias.


CONCLUSIONS
Marked changes of NMP, not only in contents but also in compositions, exist in leukemic cells compared with normal bone marrow cells. NMP may serve as a target of chemotherapeutic drugs against leukemia.
First Affiliated Hospital, Sun Yetsen University of Medical Sciences, Guangzhou.
J
J
Li
X
X
Ren
Z
Z
Huang
D034961:Antigens, Nuclear; D006801:Humans; D020014:K562 Cells / Q000378:metabolism; D015465:Leukemia, Myeloid, Accelerated Phase / Q000378:metabolism* / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism*
D034961:Antigens, Nuclear; D009687:Nuclear Proteins