Dimerization of major histocompatibility complex class I on the surface of THP-1 cells stimulates the expression of inducible nitric oxide synthase and subsequent nitric oxide release.
We show that dimerization of major histocompatibility complex (MHC) class I on a human monocytic cell line, THP-1, induces nitric oxide (NO) synthesis. Cells cultured in the presence of a human MHC class I-specific monoclonal antibody produced significant amounts of NO after 72 hr. Reverse transcription-polymerase chain reaction and flow cytometry analysis revealed that the cells synthesized detectable levels of inducible NO synthase mRNA and protein. These effects were not seen after treatment with monovalent Fab fragments or Fc fragments of the same antibody, or after treatment with a control antibody. These data show a link between innate and acquired immune mechanisms mediated by NO and MHC class I.
Department of Biological Sciences, Central Campus, University of Essex, Wivenhoe Park, Colchester, UK.
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Bottley
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Fernández
D019281:Dimerization; D015870:Gene Expression; D015395:Histocompatibility Antigens Class I / Q000502:physiology*; D006801:Humans; D018613:Microscopy, Confocal; D009000:Monocytes / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase