[Apoptosis of B lymphocytic leukemia induced by anticancer drugs and their cell cycle specificity].
OBJECTIVE
Evaluating the effect of apoptosis induced by anticancer drugs on human B lymphocytic leukemia cell lines (Nalm-6) and their cell cycle specificity.


METHODS
Nalm-6 cells were treated with various anticancer drugs for 8-24 hours. Apoptotic cells and their cell cycle specificity were measured by using hypodiploid DNA-FCM and TdT assay + DNA staining.


RESULTS
ADR, VP16, CPT, MTX, Ara-C could markedly induce apoptosis of Nalm-6 cells in S phase. CPM, PRD, 6MP were less capable of inducing apoptosis. CPM in high dose resulted in cell necrosis. PRD induced apoptosis in G1 phase, while 6MP induced apoptosis in G1 and S phase. The effect of CPM showed no marked cell cycle specificity.


CONCLUSIONS
Hypodiploid DNA-FCM and TdT assay + DNA staining can be used to detect both tumor cell apoptosis and their cell cycle specificity which is helpful to predict prognosis and to design new chemotherapy regimen.
Department of Medical Oncology, Sun Yet-Sen University of Medical Sciences, Guangzhou.
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X
Sun
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K
Saeki
T
T
Masahito
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D006801:Humans; D007399:Interphase; D015448:Leukemia, B-Cell / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents