Molecular cloning and characterization of human FGF-20 on chromosome 8p21.3-p22.
The fibroblast growth factors (FGFs) play important roles in morphogenesis, angiogenesis, tissue remodeling, and carcinogenesis. Human FGF-20 has been cloned and characterized in this study. FGF-20 encodes a 211-amino-acid polypeptide with the FGF-core domain. A strong hydrophobic region was found in the FGF-core domain of FGF-20; however, no typical N-terminal signal sequence was found in FGF-20, just as in FGF-9 and FGF-16. Total amino acid identities are as follows: FGF-20 vs FGF-9, 71.6%; FGF-20 vs FGF-16, 66.2%; FGF-9 vs FGF-16, 72.4%. Phylogenic analysis indicated that FGF-20, FGF-9, and FGF-16 constitute a subfamily among the FGF family. FGF-20 mRNA of 2.4 kb in size was detected in colon cancer cell line SW480 by Northern blot analysis. Lower levels of FGF-20 mRNA were detected in human fetal tissues and primary cancers by cDNA-PCR. The nucleotide sequence of FGF-20 cDNA is split into three parts in the human genome sequence of the chromosome 8p21.3-p22 region (Accession No. AB020858). These results indicate that the FGF-20 gene, located on human chromosome 8p21.3-p22, consists of three exons. Compared with the nucleotide sequence of FGF-20 cDNA determined in this study, one nucleotide deletion and one nucleotide substitution in the putative coding region were identified in human genome sequence AB020858.
Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Tsukiji 5-chome, Chuo-ku, Tokyo, 104-0045, Japan.
H
H
Kirikoshi
N
N
Sagara
T
T
Saitoh
K
K
Tanaka
H
H
Sekihara
K
K
Shiokawa
M
M
Katoh
D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005346:Fibroblast Growth Factors / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology; D014981:Xenopus; D029867:Xenopus Proteins
D018076:DNA, Complementary; D012333:RNA, Messenger; D029867:Xenopus Proteins; C120962:fibroblast growth factor 20, Xenopus; D005346:Fibroblast Growth Factors