Quality control of benserazide-levodopa and carbidopa-levodopa tablets by capillary zone electrophoresis.
In modern practice, the treatment of Parkinson's disease and syndrome is carried out using pharmaceutical formulations containing a combination of levodopa and a decarboxylation inhibitor (carbidopa or benserazide). Two pharmaceutical formulations were quantified by capillary zone electrophoresis using two procedures which differed only in the kind of background electrolyte used. One procedure used a 25 mM phosphate buffer, pH 2.5, while the second one used a 25 mM borate buffer, pH 8.5. The electrophoretic analysis was carried out using an uncoated fused- silica capillary, a separation voltage of 20 kV with currents typically less than 60 microA, and spectrophotometric detection at 205 nm. Calibration curves were performed for levodopa (concentration range 1-100 microg/mL), for carbidopa and benserazide (1-50 microg/mL), and the plots of the peak area versus concentration were found to be linear with a correlation coefficient better than 0.9990. Satisfactory results were obtained when commercial tablets were analyzed in terms of accuracy (98-102%), repeatability (0.6-2.0%), and intermediate precision (1.1-2.6%).
Istituto di Cromatografia del CNR, Area della Ricerca di Roma, Monterotondo Scalo, Roma, Italy.
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D001545:Benserazide / Q000737:chemistry*; D002230:Carbidopa / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007980:Levodopa / Q000737:chemistry*; D015394:Molecular Structure; D011786:Quality Control
D007980:Levodopa; D001545:Benserazide; D002230:Carbidopa