Essential role of trehalose in the synthesis and subsequent metabolism of corynomycolic acid in Corynebacterium matruchotii.
A previous paper indicated that corynomycolates synthesized by the fluffy layer fraction prepared from Corynebacterium matruchotii cells appeared exclusively as alpha-trehalose 6-monocorynomycolate (TMM) (T. Shimakata, K. Tsubokura, T. Kusaka, and K. Shizukuishi, 1985, Arch. Biochem. Biophys. 238, 497-508). In the present communication, the role of trehalose in the synthesis and subsequent metabolism of corynomycolic acids was reexamined. Consequently the following facts were clarified: (i) trehalose 6-phosphate (T-6-P), but not trehalose, stimulated corynomycolate synthesis from palmitate in the presence of ATP; the immediate product was TMM, which showed a rapid turnover. Since the turnover was blocked by addition of alpha-trehalose, only TMM accumulated among corynomycolate-containing substances. These results strongly suggested that T-6-P is an essential component as the acceptor in corynomycolate-synthetic system; (ii) TMM was the precursor not only to alpha-trehalose 6,6'-dicorynomycolate (TDM) and free corynomycolic acids but also to cell wall corynomycolate; (iii) addition of alpha-trehalose blocked the transfer of the corynomycolate moiety from TMM to cell wall corynomycolate, TDM, and free corynomycolic acids to a similar extent. These results clearly indicate that trehalose plays an essential role in the metabolism of corynomycolate after Claisen condensation and subsequent reduction in C. matruchotii.
Shimakata
T
T
Department of Biochemistry, Kawasaki Medical School, Kurashiki, Okayama, 701-0192, Japan.
Minatogawa
Y
Y
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001665:Binding Sites; D002473:Cell Wall / Q000378:metabolism; D003311:Cord Factors / Q000378:metabolism; D003352:Corynebacterium / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009171:Mycolic Acids / Q000378:metabolism*; D010084:Oxidation-Reduction; D013403:Sugar Phosphates / Q000494:pharmacology; D014199:Trehalose / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology
D003311:Cord Factors; D009171:Mycolic Acids; D013403:Sugar Phosphates; C021099:trehalose monomycolate; C013898:corynomycolic acid; C082722:trehalose-6-phosphate; D000255:Adenosine Triphosphate; D014199:Trehalose