Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts.
A method for the isolation of Echinococcus granulosus DNA from germinal layers of hydatid cysts is described. The method includes a hexadecyltrimethylammonium bromide/chloroform extraction and an adsorption to diatomaceous earth suspension. DNA suitable for polymerase chain reaction was obtained and used for parasite strain determination by mitochondrial cytochrome c oxidase I gene sequencing. Fertile and nonfertile cyst isolates from sheep, cattle, pigs, and humans were characterized. Hitherto, no direct parasite strain characterization has been made on nonfertile hydatid cysts, whereas here we report that nonfertile hydatid cysts were produced by sheep strain (G1 genotype) in sheep, cattle, and humans and by pig strain (G7 genotype) in pigs.
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, Administración Nacional de Laboratorios e Institutos de Salud, "Dr. Carlos G. Malbrán,", Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.
L
L
Kamenetzky
S G
SG
Canova
E A
EA
Guarnera
M C
MC
Rosenzvit
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification*; D004443:Echinococcosis / Q000469:parasitology* / Q000503:physiopathology; D004446:Echinococcus / Q000145:classification / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012756:Sheep; D013552:Swine
D018509:DNA, Helminth; D003576:Electron Transport Complex IV