The trans-10,cis-12 isomer of conjugated linoleic acid downregulates stearoyl-CoA desaturase 1 gene expression in 3T3-L1 adipocytes.
Conjugated linoleic acids (CLA) are a group of positional and geometric conjugated dienoic isomers of linoleic acid. The objective of this study was to determine the effects of the cis-9,trans-11 and trans-10,cis-12 isomers of conjugated linoleic acid on lipid composition and gene expression during the differentiation of mouse 3T3-L1 preadipocytes. Treatment of differentiating 3T3-L1 preadipocytes with trans-10,cis-12 conjugated linoleic acid (CLA) resulted in a dose-dependent decrease in the expression of the stearoyl-CoA desaturase 1 gene (SCD1). The expression of other adipocyte genes such as adipose P2 (aP2), fatty acid synthase (FAS), SCD2 and the key adipogenic transcription factors, peroxisome proliferator-activated receptor gamma2 (PPARgamma2) and CCAAT enhancer binding protein alpha (C/EBPalpha), remained elevated. Cells treated with trans-10,cis-12 CLA exhibited smaller lipid droplets, with reduced levels of the major monounsaturated fatty acids, palmitoleate and oleate. By contrast, the cis-9,trans-11 isomer did not alter adipocyte gene expression. Repression of the stearoyl-CoA desaturase gene expression in adipocytes by the trans-10,cis-12 isomer may contribute to the mechanisms by which CLA reduces body fat in mice.
Department of Biochemistry, University of Wisconsin-Madison, 53706, USA.
Y
Y
Choi
Y C
YC
Kim
Y B
YB
Han
Y
Y
Park
M W
MW
Pariza
J M
JM
Ntambi
D016475:3T3 Cells; D017667:Adipocytes / Q000201:enzymology*; D000818:Animals; D015153:Blotting, Western; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics*; D007536:Isomerism; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013230:Stearoyl-CoA Desaturase / Q000235:genetics*; D014280:Triglycerides / Q000378:metabolism
D005229:Fatty Acids, Monounsaturated; D007527:Isoenzymes; D012333:RNA, Messenger; D014280:Triglycerides; D013230:Stearoyl-CoA Desaturase