A new system for expressing heterologous gene in Escherichia coli regulated by oxygen consistence in the environment.
The expression of Vitreosilla hemoglobin gene (vgb) is regulated by oxygen consistence in E. coli. The gene transcription is activated under the condition of limited oxygen. A new system for expressing heterologous gene in E. coli regulated by dissolved oxygen consistence was constructed. It includes a host bacteria GJ100, which contained T7 RNA polymerase gene controlled by vgb promoter, and an expression vector on which the heterologous gene was under the control of a T7 promoter. The results indicated that E. coli thioredoxin A, IgG binding domain of Staphylococcus protein A(ZZ), snake neurotoxin, salmon calcitonin hexa-polymer, human interleukinII (IL2) and human pro-urokinase genes could be expressed efficiently. Expression level was more than 30% of the total cellular protein.
Tong
Q
Q
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai Center of Life Sciences, China.
Yang
S
S
Gong
Y
Y
D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006454:Hemoglobins / Q000235:genetics*; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013210:Staphylococcus / Q000235:genetics; D020589:Vitreoscilla / Q000235:genetics
D001426:Bacterial Proteins; D006454:Hemoglobins; D010100:Oxygen