Caveolin-1 is regulated by c-myc and suppresses c-myc-induced apoptosis.
Recent data indicating that overexpression of caveolin-1 as well as c-myc are relatively common features of advanced prostate cancer prompted us to test for potential cooperative interactions between caveolin-1 and c-myc that would be consistent with malignant progression. We used the well-characterized Rat1AmycERT cells to show that the caveolin-1 gene is down-regulated at the level of transcription by c-myc. By maintaining relatively high levels of caveolin-1 with an adenoviral vector or in stably transfected clones we show that caveolin-1 can suppress c-myc-induced apoptosis. Further we established human prostate cancer cell lines with the mycER construct and show that clones with increased caveolin-1 are more resistant to myc-induced apoptosis and have increased capacity for growth in soft agar when c-myc is activated.
Scott Department of Urology, Baylor College of Medicine, Houston, Texas 77030, USA.
T L
TL
Timme
A
A
Goltsov
S
S
Tahir
L
L
Li
J
J
Wang
C
C
Ren
R N
RN
Johnston
T C
TC
Thompson
D000818:Animals; D017209:Apoptosis; D051242:Caveolin 1; D022461:Caveolins; D002460:Cell Line; D015536:Down-Regulation; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000503:physiopathology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D051381:Rats
C495540:CAV1 protein, human; C495537:Cav1 protein, rat; D051242:Caveolin 1; D022461:Caveolins; D008565:Membrane Proteins; D016271:Proto-Oncogene Proteins c-myc