Transfer of specificity for human immunodeficiency virus type 1 into primary human T lymphocytes by introduction of T-cell receptor genes.
The introduction of genes encoding T-cell receptor (TCR) chains specific for human immunodeficiency virus into T cells of infected patients represents a means to quantitatively and qualitatively improve immunity to the virus. Our results demonstrate that the high level of TCR expression required for physiologic functioning can be reproducibly achieved with retroviral vectors encoding full-length unmodified TCR chains under the control of a strong internal constitutive phosphoglycerate kinase promoter.
Cooper
L J
LJ
Program in Immunology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington, USA. lcooper
Kalos
M
M
Lewinsohn
D A
DA
Riddell
S R
SR
Greenberg
P D
PD
D003001:Cloning, Molecular; D000939:Epitopes; D018014:Gene Transfer Techniques; D019672:Genes, T-Cell Receptor; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D011401:Promoter Regions, Genetic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D015728:Receptors, HIV / Q000235:genetics / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
D000939:Epitopes; D011948:Receptors, Antigen, T-Cell; D015728:Receptors, HIV