3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase of Legionella pneumophila transfers two kdo residues to a structurally different lipid A precursor of Escherichia coli.
The 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferase gene of Legionella pneumophila was cloned and sequenced. Despite remarkable structural differences in lipid A, the gene complemented a corresponding Escherichia coli mutant and was shown to encode a bifunctional enzyme which transferred 2 Kdo residues to a lipid A acceptor of E. coli.
Research Center Borstel, Center for Medicine and Biosciences, Division of Medical and Biochemical Microbiology, D-23845 Borstel, Germany. wbrabetz@fzborstel.de
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D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D016952:Legionella pneumophila / Q000201:enzymology* / Q000235:genetics; D008050:Lipid A / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014166:Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D008050:Lipid A; D011994:Recombinant Proteins; D014166:Transferases; C019917:3-deoxy-D-manno-octulosonate transferase