Transformation of amoxapine by Cunninghamella elegans.
We examined Cunninghamella elegans to determine its ability to transform amoxapine, a tricyclic antidepressant belonging to the dibenzoxazepine class of drugs. Approximately 57% of the exogenous amoxapine was metabolized to three metabolites that were isolated by high-performance liquid chromatography and were identified by nuclear magnetic resonance and mass spectrometry as 7-hydroxyamoxapine (48%), N-formyl-7-hydroxyamoxapine (31%), and N-formylamoxapine (21%). 7-Hydroxyamoxapine, a mammalian metabolite with biological activity, now can be produced in milligram quantities for toxicological evaluation.
Moody
J D
JD
Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas 72079, USA.
Zhang
D
D
Heinze
T M
TM
Cerniglia
C E
CE
D000657:Amoxapine / Q000378:metabolism*; D018687:Antidepressive Agents, Second-Generation / Q000378:metabolism*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D020093:Cunninghamella / Q000254:growth & development / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry
D018687:Antidepressive Agents, Second-Generation; D000657:Amoxapine