Detection of factor V Leiden in Thai patients with venous thrombosis.
The molecular defect underlying activated protein C resistance (APC-R) is caused by a G to A point mutation in the codon for arginine 506 in the factor V gene (factor V Leiden) which is a major risk factor for venous thrombosis, especially in Caucasian populations. This study is an analysis of the Thai population to determine the prevalence of the factor V Leiden mutation. Twenty-seven patients with apparent venous thrombosis were divided into two groups according to APC-R test. Thirteen patients were diagnosed as positive for n-APC-SR, ratio < 0.8 and fourteen patients were diagnosed as negative for n-APC-SR, ratio > 0.8. Two of thirteen APC-R positive patients and one of fourteen APC-R negative patients were found to have the heterozygous allele for the factor V Leiden mutation but the homozygous allele was not detected in these groups of patients. Neither the heterozygous nor homozygous Leiden mutation was detected in 200 healthy volunteer blood donors. In conclusion, our findings indicate that factor V Leiden mutation is related to venous thrombosis in Thai people. Moreover, a further study of other mutations at the activated protein C cleavage sites of factor V and factor VIII is recommended.
Prayoonwiwat
W
W
Department of Medicine, Phramongkutklao College of Medicine, Bangkok, Thailand.
Arnutti
P
P
Hiyoshi
M
M
Nathalang
O
O
Suwanasophon
C
C
Kokaseam
R
R
Krutvecho
T
T
Tatsumi
N
N
D020016:Activated Protein C Resistance / Q000235:genetics; D000328:Adult; D000368:Aged; D000483:Alleles; D001778:Blood Coagulation Disorders / Q000235:genetics; D005165:Factor V / Q000032:analysis / Q000235:genetics*; D005828:Genetics, Population; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D015995:Prevalence; D013785:Thailand; D020246:Venous Thrombosis / Q000453:epidemiology / Q000235:genetics*
D005165:Factor V