Construction of mutants of Sphingomonas paucimobilis defective in terminal mannose in the glycosphingolipid.
Mutants of Sphingomonaspaucimobilis defective in a part of the carbohydrate moiety of the glycosphingolipid (GSL) were constructed by transposon (Tn5)-insertional mutagenesis. Defective mutants were selected by ELISA using the antibody recognizing the tetrasaccharide-type GSL (GSL-4A) of S. paucimobilis. Eight defective mutants were selected from about 8,000 kanamycin-resistant strains, and seven of them were found to lack the terminal mannose of GSL-4A. The chemical structure of the mutant GSL was investigated, and proved that the rest of the structure was not changed by the mutation.
Department of Microbiology, School of Pharmaceutical Sciences,The Kitasato Institute, Kitasato University, Tokyo, Japan.
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Kaneko
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Miyadai
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Danbara
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Kawahara
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006028:Glycosphingolipids / Q000737:chemistry* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020578:Sphingomonas / Q000737:chemistry* / Q000235:genetics*
D002241:Carbohydrates; D005227:Fatty Acids; D006028:Glycosphingolipids; D008358:Mannose