Accumulation of a natural substrate of laccase in gills of Pleurotus florida during sporulation.
During sporulation, laccase activity of Pleurotus florida decreased to a minimum level in spite of increase in the number of isozymes. An endogenous laccase substrate was detected especially in the gill structure of the sporophore, which competitively inhibited oxidation of guaiacol by the enzyme during in vitro assay. Appearance of the laccase substrate in the gill structure may be linked with the sporulation phenomenon.
Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Calcutta-700 032, India.
T K
TK
Chakraborty
N
N
Das
S
S
Sengupta
M
M
Mukherjee
D006139:Guaiacol / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D042845:Laccase; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020076:Pleurotus / Q000737:chemistry / Q000201:enzymology*; D013170:Spores; D013379:Substrate Specificity
D007527:Isoenzymes; D006139:Guaiacol; D010088:Oxidoreductases; D042845:Laccase