Long-term role of nitric oxide in the enteric nervous system of the transplanted rat intestine.
We investigated the long-term changes of the nitric oxide (NO)-related neural component after syngeneic total small bowel transplantation in rats. In the present study, the NO-related neural component was examined using the electrophysiological and NADPH-diaphorase histochemical technique. The rats were divided into four groups: an untreated young adult control group, an untreated 2-year-old control group, a group killed 1 month after transplantation, and a group killed 2 years after transplantation. A superfusion apparatus was used to evaluate the response of jejunal strips to electrical transmural stimulation. In the presence of adrenergic and cholinergic blockade, the inhibitory effect of L-N(G)-nitro arginine (L-NNA; a nitric oxide synthesis inhibitor) on nonadrenergic, noncholinergic (NANC) relaxation was expressed as a L-NNA-sensitive component. The L-NNA-sensitive component accounted for 41.6 +/- 4.6% (mean +/- SE), 43.1 +/- 3.5%,54.6 +/- 4.1%, and 55.8 +/- 3.5% in the young control group, 2-year control group, 1-month transplant group, and 2-year transplant group, respectively, being significantly higher in the transplant groups (p < 0.05). The actual strength of the L-NNA-sensitive component was 0.24 +/- 0.03 (mean +/- SE), 0.26 +/- 0.02, 0.44 +/- 0.04, and 0.46 +/- 0.04 mg of tension per mg of wet weight, respectively, also being significantly higher in the transplant groups (p < 0.001). In addition, the percentage of NADPH-diaphorase-positive fibers was 24.1 +/- 1.1% (mean +/- SE), 25.5 +/- 1.4%, 31.0 +/- 1.6%, and 30.9 +/- 2.0%, respectively, being significantly higher in the transplant groups (p < 0.05). These results suggest that NO neurons in the intrinsic jejunal nervous system have an adaptive role in maintaining intestinal graft motility.
Ishii
H
H
Second Department of Surgery, Hyogo College of Medicine, Nishinomiya, Japan.
Nakao
K
K
Nishizaki
K
K
Ichii
S
S
Hatada
T
T
Yamamura
T
T
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D008058:Dihydrolipoamide Dehydrogenase / Q000032:analysis; D004558:Electric Stimulation; D017615:Enteric Nervous System / Q000502:physiology*; D006145:Guanethidine / Q000494:pharmacology; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000294:innervation / Q000502:physiology* / Q000637:transplantation*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000294:innervation / Q000502:physiology* / Q000637:transplantation*; D009569:Nitric Oxide / Q000502:physiology*; D019335:Nitroarginine / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D014185:Transplantation, Isogeneic / Q000502:physiology*
D019335:Nitroarginine; D009569:Nitric Oxide; D001285:Atropine; D008058:Dihydrolipoamide Dehydrogenase; D006145:Guanethidine