Characterization of substance P-membrane interaction by transferred nuclear Overhauser effect.
Substance P, one of the mammalian tachykinins, is known to interact strongly with lipid bilayers and this interaction may play a role in the receptor-peptide recognition process. The conformation of substance P bound to vesicles consisting of perdeuterated phosphatidylcholine has been investigated by means of two-dimensional transferred nuclear Overhauser (trNOE) spectroscopy. Nuclear magnetic resonance data analysis resulted in a unique conformational family characterized by a well-defined conformation of the last seven C-terminal amino acids, which consists of a sequence of nonstandard turns following each other in a helix-like manner. The absence of short- or medium-range trNOE in the N-terminal part indicates its structural flexibility.
Augé
S
S
Institut de Pharmacologie et de Biologie Structurale, CNRS, 205 rte de Narbonne, 31077 Toulouse, France.
Bersch
B
B
Tropis
M
M
Milon
A
A
D001483:Base Sequence; D003903:Deuterium; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D017711:Nonlinear Dynamics; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017434:Protein Structure, Tertiary; D013373:Substance P / Q000737:chemistry* / Q000378:metabolism
D008081:Liposomes; D013373:Substance P; D003903:Deuterium