Specific neurons of brain cortex and cerebellum are PIPPin positive.
We recently cloned a cDNA encoding an RNA-binding protein, that we called PIPPin, which is highly enriched in the rat brain and contains two putative double stranded RNA-binding domains (PIP1 and PIP2) and a central cold shock domain (CSD). Here we report that PIPPin is specifically enriched in some pyramidal neurons of the cerebral cortex and in the Purkinje cells of the cerebellum. We also show that PIPPin inhibits translation of H1(o) and H3.3 mRNA in a cell-free system. The results reported suggest that PIPPin down-regulates histone variant expression in the developing rat brain.
Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, Palermo, Italy.
T
T
Nastasi
P
P
Muzi
S
S
Beccari
M
M
Bellafiore
V
V
Dolo
M
M
Bologna
A
A
Cestelli
I
I
Di Liegro
D000818:Animals; D002531:Cerebellum / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D014176:Protein Biosynthesis; D011689:Purkinje Cells / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
C097845:Csdc2 protein, rat; D006657:Histones; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins