Topical acetyl salicylate and dipyrone attenuate neurogenic protein extravasation in rat skin in vivo.
The effect of topically applied acetyl salicylic acid (ASA) and dipyrone on capsaicin-evoked protein extravasation was investigated by dermal microdialysis in rat. After a baseline of 75 min, capsaicin (1%) was applied epicutaneously under occlusion for 75 min above the capillaries. Topical capsaicin stimulation induced neurogenic protein extravasation with a mean increase of protein concentration in the perfusate of 165+/-27% (mean+/-SEM; n=15), whereas in sham-stimulated sites protein concentration decreased to 73+/-7% of the prestimulation value (n=6). ASA (2-200 mg/ml) and dipyrone (3-300 mg/ml) dose-dependently reduced the capsaicin induced protein extravasation to 118+/-23% (ASA, 200 mg/ml; n=8) and 72+/-9% (dipyrone, 300 mg/ml; n=8) of the prestimulation value. ASA and dipyrone antagonized the excitatory effects of capsaicin on skin nociceptors and thus suppressed the neurogenic protein extravasation.
Department of Physiology and Experimental Pathophysiology, Universitätsstrasse 17, 91054 Erlangen-, Nürnberg, Germany. schmelz@physiologie1.uni-erlangen.de
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D000287:Administration, Topical; D000636:Aminosalicylic Acids / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D002211:Capsaicin / Q000008:administration & dosage / Q000037:antagonists & inhibitors; D004177:Dipyrone / Q000008:administration & dosage* / Q000494:pharmacology; D008297:Male; D020078:Neurogenic Inflammation / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism*
D000636:Aminosalicylic Acids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004177:Dipyrone; C028836:N-acetyl-5-aminosalicylic acid; D002211:Capsaicin