Genomic subpopulations within the species Pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin AcH/PA-1 producing and non-producing strains.
A high degree of genetic polymorphism among P. acidilactici strains was highlighted by a multilocus typing approach analysing several housekeeping genes and by sampling the whole genome using random amplified polymorphic DNA (RAPD) fingerprint analysis performed by using a single primer pedA gene targeted in low-stringency amplification conditions. Restriction fragment length polymorphism of the rpoC, ldhD/L and mle genes, and a modified RAPD analysis, permitted the grouping of Pediococcus acidilactici strains in seven genotypes (I-VII). Genotypic results obtained by analysing housekeeping genes involved in the transcription/translation machinery and in primary metabolism were supported by phylogenetic analysis based on the partial 16S rDNA sequencing of a reference strain of each of the seven clusters obtained. Three of the seven genotypes detected showed relationships with pediocin AcH/PA-1 production and carbohydrate fermentation patterns: all pediocin-producing and sucrose-positive strains were grouped in genotype VII, melibiose-, sucrose- and raffinose-positive strains in genotype VI, and arabinose-positive strains in genotype V.
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale1 and sezione Microbiologia Agraria, Alimentare e Ecologica2, Università di Milano, via Celoria 2, 20133 Milano, Italy.
Diego
D
Mora
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale1 and sezione Microbiologia Agraria, Alimentare e Ecologica2, Università di Milano, via Celoria 2, 20133 Milano, Italy.
Maria Grazia
MG
Fortina
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale1 and sezione Microbiologia Agraria, Alimentare e Ecologica2, Università di Milano, via Celoria 2, 20133 Milano, Italy.
Carlo
C
Parini
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale1 and sezione Microbiologia Agraria, Alimentare e Ecologica2, Università di Milano, via Celoria 2, 20133 Milano, Italy.
Daniele
D
Daffonchio
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, sezione Microbiologia Industriale1 and sezione Microbiologia Agraria, Alimentare e Ecologica2, Università di Milano, via Celoria 2, 20133 Milano, Italy.
Pier Luigi
PL
Manachini
D015373:Bacterial Typing Techniques; D001430:Bacteriocins / Q000096:biosynthesis*; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016680:Genome, Bacterial; D019344:Lactic Acid / Q000096:biosynthesis; D000070017:Pediocins; D010376:Pediococcus / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique
D001430:Bacteriocins; D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D000070017:Pediocins; C074288:pediocin PA-1; D019344:Lactic Acid