High-resolution genotyping of Chlamydia trachomatis from recurrent urogenital infections.
A method for Chlamydia trachomatis restriction fragment length polymorphism (RFLP) analysis and complete sequencing of omp1 was developed for use on samples collected at home, and results were compared. Genotyping by sequencing was superior to RFLP analysis. The omp1 gene in 31 clinical strains harbored few mutations compared to the same gene in ATCC reference strains. Follow-up samples obtained during a 24-week period from 31 patients showed recurrence with the same genotype in five cases and a new genotype in one case.
Department of Clinical Microbiology, Aarhus Municipal Hospital, DK-8000 Aarhus, Denmark. Akh.grp02s.lhp@aaa.dk
L N
LN
Pedersen
H O
HO
Kjaer
J K
JK
Møller
T F
TF
Orntoft
L
L
Ostergaard
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D052776:Female Urogenital Diseases / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases; D012150:Polymorphism, Restriction Fragment Length; D018272:Porins; D012008:Recurrence; D012648:Self Care; D017422:Sequence Analysis, DNA; D013048:Specimen Handling; D014556:Urine / Q000382:microbiology
D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis