[Position effect variegation of the mosaic type, arising as a result of transposition AR4-24P[white, rosy] in the Drosophila melanogaster genome].
A line with the mosaic expression of the white+ transgene was obtained by inducing transposition of the AR4-24P[white, rosy] transposon and was used for the second round of induction. As a result, 57 lines with the mosaic eye pigmentation were obtained. In situ hybridization and Southern blotting showed that genomic DNA fragments flanking AR4-24 were, in some cases, transposed together with the transposon. A spontaneous loss of these fragments resulted in reversion to the wild-type phenotype. The mosaic eye pigmentation in a line that carried the AR4-24 transposon flanked with the same fragments in region 24D1-2 was not affected by the Su(var)3-6 gene modifying position effect variegation (PEV). Other PEV modifiers, Su(var)3-9 and Su(var)2-5, had only a slight effect on PEV; Su(var)3-7 restored the wild-type phenotype. The genomic fragments captured by the transposon may contain DNA sequences that autonomously induce mosaic PEV of the white gene.
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia.
M L
ML
Balasov
E S
ES
Beliaeva
S A
SA
Shestopal
I V
IV
Makunin
I F
IF
Zhimulev
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D016678:Genome; D017403:In Situ Hybridization; D009030:Mosaicism; D012168:Retinal Pigments / Q000235:genetics; D019076:Transgenes
D004251:DNA Transposable Elements; D012168:Retinal Pigments; D004247:DNA