Cutting edge: essential role of phospholipase C-gamma 2 in B cell development and function.
Cross-linking of the B cell Ag receptor (BCR) induces the tyrosine phosphorylation of multiple cellular substrates, including phospholipase C (PLC)-gamma 2, which is involved in the activation of the phosphatidylinositol pathway. To assess the importance of PLC-gamma 2 in murine lymphopoiesis, the PLC-gamma 2 gene was inducibly ablated by using IFN-regulated Cre recombinase. Mice with a neonatally induced loss of PLC-gamma 2 function displayed reduced numbers of mature conventional B cells and peritoneal B1 cells and defective responses in vitro to BCR stimulation and in vivo to immunization with thymus-independent type II Ags. In contrast, T cell development and TCR-mediated proliferation were normal. Taken together, PLC-gamma 2 is a critical component of BCR signaling pathways and is required to promote B cell development.
Department of Molecular Genetics, Institute for Liver Research, Kansai Medical University, Moriguchi, Japan.
A
A
Hashimoto
K
K
Takeda
M
M
Inaba
M
M
Sekimata
T
T
Kaisho
S
S
Ikehara
Y
Y
Homma
S
S
Akira
T
T
Kurosaki
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D000955:Antigens, T-Independent / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology* / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D005362:Ficoll / Q000031:analogs & derivatives* / Q000276:immunology; D017353:Gene Deletion; D006241:Haptens / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis; D007527:Isoenzymes / Q000235:genetics / Q000502:physiology*; D008213:Lymphocyte Activation / Q000235:genetics; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D051966:Phospholipase C gamma; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology; D010738:Type C Phospholipases / Q000235:genetics / Q000502:physiology*
D000954:Antigens, Surface; D000955:Antigens, T-Independent; D006241:Haptens; D007075:Immunoglobulin M; D007527:Isoenzymes; C020529:TNP-ficoll; D014301:Trinitrobenzenes; C054401:trinitrophenyl keyhole limpet hemocyanin; D005362:Ficoll; D006433:Hemocyanins; D010738:Type C Phospholipases; D051966:Phospholipase C gamma; D002118:Calcium