[Detection of fetal cells in maternal blood by fluorescence in situ hybridization].
OBJECTIVE
To isolate fetal nucleated cells from maternal blood and determine its fetal origin.


METHODS
Enrichment and isolation of nucleated cells in maternal blood from 20 samples in the first trimester of pregnancy, 20 samples in the mid-trimester of pregnancy, and 15 samples after delivery. Fluorescence in situ hybridization was performed using Y specific probe PY3.4 to identify fetal cells.


RESULTS
Fifteen women in the first trimester and 15 women in the mid-trimester of pregnancy carried male fetuses. The rates of positive cells were 1:6528.0 and 1:2783.8 respectively, and there was a highly significant difference when these rates were compared with the positive cells rate of the 10 female fetuses in the same trimesters. No significant difference in positive cells rate was found between 1 week after delivery and the mid-trimester of pregnancy, nor was it found between 3 months after delivery and the 10 samples of female fetuses in the same time.


CONCLUSIONS
These data suggest that fetal cells can be detected as early as from 50 days of gestation in maternal blood, in the meantime, the rate of fetal cells will increase with gestational age. One week after delivery, fetal cells still exist, and 3 months after delivery, the fetal cells will no longer be detected.
General Hospital, Tianjin Medical University, Tianjin, P. R. China. WPZZLW@PUBLIC.TPT.TJ.CN
S
S
Gu
Y
Y
Zhang
C
C
Ren
X
X
Zhang
K
K
Shi
H
H
Jiang
S
S
Guan
Y
Y
Li
D005260:Female; D005333:Fetus / Q000166:cytology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy; D011296:Prenatal Diagnosis; D013997:Time Factors